LOCUS       DAORPM010000001        26941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_271_length_26941_cov_11.343376, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000001 DAORPM010000000
VERSION     DAORPM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 26941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26941
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_271_length_26941_cov_11.343376"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLD53_00005"
     CDS             complement(<1..387)
                     /locus_tag="PLD53_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRC59313.1"
                     /translation="MSYASKYRAIADDLCCRLQAVGHEVFFDREDLRAGASFDDRIRS
                     AIEDCELFIFLISPEAVAAGHYTRTEVKIASRKWPTPGWHVLPVQVAETPLADIPAYL
                     RALTIMQAEGNLAAEVVLEVEDRVRAN"
     gene            685..2424
                     /locus_tag="PLD53_00010"
     CDS             685..2424
                     /locus_tag="PLD53_00010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRC59314.1"
                     /translation="MRLFSFLSLRQWLTLPYIALVFGVAVLIGALSYRTGSQAVDTVA
                     HHLLLETVARIGQAVDRHVVGSAAALEAAFPNGMPAPDTIDADLPALRTRFWIATSLH
                     IDPNNYVYYGNRHGQFFGLWRFNVQDAELRLKLQADRPRQKFRFSGINGVPSEPTTEQ
                     KMYEPRARPWYKSGEDNLSHTWTSIYIDFTTAELVATRARRVLDAQGGLQGVVATDLS
                     LKRLNDFVRKLSLSDNAVAFIIEPDGKLIASSRSANTMPHPDGTNARINASDSDNELQ
                     RAAYAQVLGFAAAGNKIDRALTQRFAGPNDQVVELALDRVRDDAGLDWIIVVAVPRSD
                     FMQGVTANVQRTVVIGVFGALAAVAIGLSILGWVGRDIRRLTQAAQDVGEGRLEVPLD
                     VHRNDEIGVLANTFRHMQHRLRTDLLTGLGNRDVMIRSISSRIEKARRTGDSMPFAVL
                     FVDLNNFKLINDRFGHDAGDRVLMEIGNRLLKASRACDRVARYAGDEFLLMINDIPSR
                     QVAEQIRHNIEELLREPLQTVDVSSLPEAVLMSGSVGMAFYPDDGDNADELIKRSDHD
                     MFERKRTWQSDCI"
     gene            complement(2449..2901)
                     /locus_tag="PLD53_00015"
     CDS             complement(2449..2901)
                     /locus_tag="PLD53_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRC59315.1"
                     /translation="MKIVKLVVALTLALLVPVASAADGLVSVKSPYSAKETMNRLEEL
                     VKKRGLKVFARIDHAAGAASIGKSLRPTELLIFGNPQGGTPFMECAQSAGIDLPLKAL
                     VWEDASSQVWLSYNAPAFLAKRHDVAACPVVENLSKALAGIAEATVAK"
     gene            3079..3600
                     /locus_tag="PLD53_00020"
     CDS             3079..3600
                     /locus_tag="PLD53_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRC59316.1"
                     /translation="MACLDSGSQSDALTPLNQFQDAFMLKTLKDLFDSFTGDSPDSSP
                     RAREHALQLATAVLLVEVMRADPAIEAAERTAVLAALKAKFALADDESDRLLEVAEQT
                     AKDAYDYHRFTNSINASFTHEEKVRVIESMWQVAYADGHLDSHENHLISKVASLLHVT
                     HREYIAAKLRAKC"
     gene            complement(3675..5276)
                     /locus_tag="PLD53_00025"
     CDS             complement(3675..5276)
                     /locus_tag="PLD53_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRC59317.1"
                     /translation="MTEFSGLTLSVDDNRLIAYISASEARAFLEPDSLRALLAEAGFG
                     SWHLSEGALQALIEHFNSSTSELKLPIGERSDASFKLEMAQDAMQVWADFIPSYGGIA
                     LDPNDVYQALEETGVVVGIDSSAVSAACASDSAERILVAAGQPAEDGIDAHFEMLVAD
                     TRDRTPQVSENGLIDFRELGAIPMVAAEQPLMRRIPPTTGTAGRNVRGEVIEPVPGQD
                     ALFDEGLIGAYVDKEDANLLRAVFSGQPVRAGNGVNVEQVLNISHVNMASGNISFDGT
                     VNIEGDVSPGMKVHASGDIVVGEVVDGAELDAGGDIRVAGGIISQARVHAGGSVSARF
                     VESAHVFSGTIIAIDDSALQSELQANNQIVVGVSSPQRGRLAGGSAKAMMLIKTPILG
                     SQTGGVTNLVLGVNPVLEAQYQDLLKVIEKQREEESKLEMLVKHLTKIGDKNGMLERV
                     KASWEQAIKAWAQLLPQRDELERQLALIAGARVEIGLNVVGAVDILFGKKVSRLRKTY
                     ETGSFSIEGDRVLFTDPEGNVSPAA"
     gene            complement(5538..7028)
                     /locus_tag="PLD53_00030"
     CDS             complement(5538..7028)
                     /locus_tag="PLD53_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC59318.1"
                     /translation="MIGGRSFTTRLTLALSLLLLAYGALVGLLGRHVAVEYEHESLQR
                     LSHGLAKHIVEHWPEIAVADPDQADRAARESLLSMLMVVNPGIQVYVLDADGRVQAYI
                     GDPGMVREKQVDLGAVRSFLAGAALPLRGTDPMGTGIPRIFSAAMFPPRAGDVRPPGY
                     LYVVLDGQARVHVAGQLSLRRVWQGAGLVAAAGLLVTLLLGFFAMHRLTLPLHRLANR
                     MRDYSVRTLQATAAGRSQSRTNGDEVSAIGSAFDEMTQRIEAQTAREQQQASAHREVM
                     ASVAHDMRTPLTALHGHLEAMAGQALVEPEQRDRVLAAALAQSDKVRRLSQQLFELAA
                     LQSTDQVLHRERFRLDELVTDAVQKYDLADGPAPVTLRGAPPGPLEFEGDLQLIERAL
                     TNLIDNAMRHAPTTSPIHVSVSRDGAQANILIEDNGPGLPDELFSRLEQGKSLRDPPV
                     KRTSGGIGGLGLAIAQRVAALHGGSLQPLPSPLGGTRLCLALPLAE"
     gene            complement(7025..7756)
                     /locus_tag="PLD53_00035"
     CDS             complement(7025..7756)
                     /locus_tag="PLD53_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC59319.1"
                     /translation="MPLPCGAGFAVNEQLLLVEDDDAISGALRLHLEEAGYRLHRESD
                     GLRAMAAIDRQRWDMVLLDLMLPGADGWEVCRHLRARHADVPVIMLSARSAEAHRVLG
                     LELGADDYLAKPFSMLELVARVRALLRRIEQLRSSPAAASEVGFGPFRLDTLRRELLR
                     GNDTVPLTLREFDLLHFLVRHPGRAFSRNELLQRVWGAAFDGYEHTVNSHINRLRTKI
                     EDNPRDPHRIVTVWGVGYRFDANPS"
     gene            complement(7794..8603)
                     /locus_tag="PLD53_00040"
     CDS             complement(7794..8603)
                     /locus_tag="PLD53_00040"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HRC59320.1"
                     /translation="MNLISSTSLRRAAGIAALVLSATTSAAQLNILVKVENLAPANSI
                     SFAPLHVGFNNGTFDSFNNGQAATAAIISVAEGGSGSAWQPAFAAADPSATRGTIGGL
                     LQPGQTSSFSFLVDTALNRFFTFASMVVPSNDFFIGNDSPTQYQLFDAFGTLVIGSIV
                     QKASDIWDAGSELFDPAAAAFVGNNDLRTDQNGDVSFNFAELAGFDGLTTATGYVFNS
                     ALTADTDVYRITFQAVPEPETIALMVAGLLAVAWISRRRRGQSFESGSELV"
     gene            9038..10198
                     /locus_tag="PLD53_00045"
     CDS             9038..10198
                     /locus_tag="PLD53_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRC59321.1"
                     /translation="MPELVGSIVAILLAGLLMHELRQPNVIAYLIAGIMLGPSLFGAI
                     TDQQVLDRLGQFGVVMLMFFIGMEVSPEKVIARWRVALLGTFMQIVVSVALTTAIGHF
                     FDWPFVLCVLLGFIISLSSTAVVLNLLQARKELETRHGQNALNVLLAQDIAVIPMLIV
                     VSILSGEKVSRMEMTMQVIGGILLIACCAWIVIRPGFRIPLIGDAVKKDHELQVFGAM
                     LLCFGLALLTGLFSLSSALGSFVAGIVVAAAKEAKWVHHSLEPFKTVFVALFFVSVGM
                     LVDIDFVLHHWGKVLSLALIALTVNMAINATVFKILGETWGDSLYTGAILAQIGEFSF
                     VLAAVGASSMIISKENYQLAVAVIVVSLLLSPLLISRMRRYALGGDELHKEP"
     gene            complement(10223..10627)
                     /locus_tag="PLD53_00050"
     CDS             complement(10223..10627)
                     /locus_tag="PLD53_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRC59322.1"
                     /translation="MPAPERLPLPSLRIDKWLWAARFYKTRSMATEAVHSGHVKLNGC
                     GVKPARELRIGDTLGITVGDTLWTITVCGLNDQRRPASEAQQLYAETPESAAHRNAER
                     ESKRLAPTPGSDLRGRPSKKARRQIRRFNEEY"
     gene            complement(10646..13738)
                     /locus_tag="PLD53_00055"
     CDS             complement(10646..13738)
                     /locus_tag="PLD53_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC59323.1"
                     /translation="MNLPELCIRRPVMTTLLMAAFVIFGIIAYRALPVSELPSVDFPT
                     ISVTAALPGASPETMAAAVATPLEGQFSTIAGLDSMNSTSAQGSTSITLQFTLDRNID
                     AAAQDVQSAIAVALRKLPPNMPAPPSFRKVNPADSPIYYIALASPTLPLPLVNEYAET
                     QLAQRISTISGVAQVLIYGSQKFAVRIQANPDQLAARGLGIDELHQAVAQANVNQPVG
                     LIDGERRTFAIKDNGQLDNSVAYRPLIVAWRNGAPVRLEEVATPIDSVENLRLASWNV
                     DKRAIVLAIQRQPGANTIETVDSIKRILPSFQAKLPAAISMTTLYDRSVSIRHAIEDV
                     QFTLILAGFLVILVILLFLRNLYATLIPALALPISVIGTFAVMSMLGFSLDNLSLLAL
                     TLSVGFVVDDAIVMLENIVRHIELGDSPMHAAIKGSREIGFTIISMTVSLVAVFIPVL
                     FMGGIVGRLLHEFAVTICAAILVSGVVSLTLTPMMCSRYLKPSRTEDHGRLFRAFEQF
                     FAALLAGYEKSLRQAIAHPRLVVAGFVATAGLSGLLFSMVPKDFIPSGDSGRIIAFTE
                     GAQDASFASMVEHQRAVAAIVAEDPNIQSFMSSIGGGGIRPTSNTGTIFMILKPSGER
                     TLSPDELIEQLRPRLATVPGIKVYMQNPPVIRIGGLITAAQYQYTLQSTDLHELYQWT
                     ETLLGRIRQLPGFVDVTSNLNNHSPVVSLDIDRDKITTLGLTFSQVEDALQSAFSARQ
                     ISTIYGTANQYQVILEVAPEFQSDPSMLSRLHVRASSGKLIPLDTIAGISRKTQALTV
                     NHQGQLPAVTLSFNLLPGISLGEAVTRIKALEQELRIPASLNTSLQGTAQAFQASLQG
                     LGILLLIAILVVYIVLGILYESFIHPLTILSGLPSAALGALITLMVFRVDLSLYAFVG
                     IIMLIGIVKKNAIMMIDFALDQQRSSQLPAHETIFNACLIRFRPIMMTTMAALVGTLP
                     IAIGFGAGADVRRPLGLAVVGGLLLSQFLTLYLTPVIYVYLDRFTPKGTDLPAMPETR
                     "
     gene            complement(13750..14913)
                     /locus_tag="PLD53_00060"
     CDS             complement(13750..14913)
                     /locus_tag="PLD53_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC59324.1"
                     /translation="MKTVRITFRYAFLSAGLVILTACSEGDTEKKPPPPVPVIAVKAT
                     EQDIPVGLRVVGRAEAFASVVLKSRIDGQVAEVLFTEGQHVKQGDILIRLDPSDFSAR
                     LRQAEASVARDESLAAKTRADTQRYMALKDRNFVSAEKVNEIRTNESAAAASLRASKA
                     AAELARLQLSYATIRAPFDGVVGARLVFPGSSVKINDTTLAVVNRVRPLLVSFSVPEK
                     YLPQLRAALGHGTATLDGLRIEVNVPQNDSQRFVGRVRFLDNTVDSATGTIQMKAELP
                     NDDETLTPGQFLNVTLLLQTLTKVVAVPDEAIQQGASGNVVFVVKDDGSVEVRQVETA
                     ASDGGMTAIGKGLNVGETVVTDGQLRLAPGTRVIVRYADEPGSAEASGKPANH"
     gene            complement(14910..16154)
                     /gene="lysA"
                     /locus_tag="PLD53_00065"
     CDS             complement(14910..16154)
                     /gene="lysA"
                     /locus_tag="PLD53_00065"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRC59325.1"
                     /translation="MDAFSLSNGQLLAESVPLADIAARFGTPCYVYSRAALEAALDEF
                     QQELTGIDSLVCYALKANSSLAVLNIFARRGAGFDIVSIGELQRALAAGADPKKIVFS
                     GVGKSAPEMAYALDTGILCFNVESAPELDRLNEVAGGLKKKAPISLRVNPDVDAKTHP
                     YISTGLKENKFGVAYEDALEVYQHAACLPNLEITGIDCHIGSQLLDPAPFAEALDKLL
                     LLIDQLAARGIHLHHIDLGGGLGIRYKDEEAPTVKDYLRPLLQALKDRSLKILFEPGR
                     RLVGNAGVLLTRVEYLKPGEAKNFAIVDAAMNDLARPALYDAWHDIVAVSPRSVAPRD
                     WDIVGPICESGDFLGRNRSLAIEPGDLLAVMSAGAYGMVMSSNYNTRPRAAEVMVDGT
                     EVHLIRRRETVEELYAHETLLP"
     gene            16385..16702
                     /gene="cyaY"
                     /locus_tag="PLD53_00070"
     CDS             16385..16702
                     /gene="cyaY"
                     /locus_tag="PLD53_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HRC59326.1"
                     /translation="MNDSEFNALADLALTRIENGLESSGADLDFAMVSAGVFEIEFAD
                     GSKIIVNRHGAAQELWVAARSGGFHYRWDGQNWRDTRDGTDLMATLAKLVSAQAGEPV
                     QLN"
     gene            complement(16712..18946)
                     /locus_tag="PLD53_00075"
     CDS             complement(16712..18946)
                     /locus_tag="PLD53_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HRC59327.1"
                     /translation="MVIAMAAVVLILTYPNLPSLEILTDYRPKIPLRVYTAEGQLIGE
                     FGEEHREVVRFQDVPDVMKQAILAAEDERFYQHRGVDVLGIARAALSNLTGGGKRQGA
                     STITQQVAKNFFLSSEKTYTRKLYEALLSFKIENSLSKDQIFELYINQIFLGQRAYGF
                     GAAAQIYFGKPLADLSIAEAAMLAGLPKAPSAFNPVVNPKRARLRQQYVLRRMLELGF
                     IDPKQHEAAMKQALVVKRDTTVYAVHAEFVAEMARQLAAERFPDDVYSRGLSVYTTIL
                     NGDQEAAYRSLRRGVFDYDRRHGYRGAEAYVDMSGIKSEQDEALDEVLQDFHDSEDLH
                     PAVVLQADAKQIRAYRRGGEIVTIGGDGLKFALRMMDDKAPPNKRLRRGAVIRIQKDD
                     NGNWRITQMPEVEAAFLATDPRNGAIRALVGGFDYNRNKFNHATQAWRQPGSSFKPFI
                     YSGALEKGFTPASIIEDAPISIPASVTGSQAWEPKNYDGKYAGPMRMRTALAKSKNMV
                     SIRLLQASGVNYIQDYATRFGFDADKHPPYLTMALGAGAATPWQMVAAYSVFANGGYR
                     IQPFIVRDIRDDKGQVLAEAQPITAGDESLRAIDPRNAYLMDSMLRDVTIYGTGARAS
                     ATLKRRDLAGKTGTTNEYVDAWFCGYQQTVVGCSWIGFDQPRTLGKGETGGSAALPTW
                     IGYMAVALKDVPQTFMSQPDGLVALEIENPGKGPAREFFYKENVPPVIEIEPPQDPDD
                     VPID"
     gene            19196..20275
                     /locus_tag="PLD53_00080"
     CDS             19196..20275
                     /locus_tag="PLD53_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRC59328.1"
                     /translation="MQLDLSIFNPKARSLIGLDISSSAVKMVELASDGKSGYRVERYT
                     IEVLPRDAVADGNIVNLEAAAETVRRAWKKLATSTRHVAIALPASHVITKKIIVAAGQ
                     REEELGVQVESEANQYIPFALEEVNLDFQVVGPAASSPDEIEVLIAASRKEKVEDRVA
                     VAESAGLKPVVMDVESYAVLSAFELIEKQLPDGGNGQIVALVDVGANVMNLTVLRNGQ
                     QLYVREQAFGGNQLTQDIARLFGMNFEEAEAEKRRNNLPDNYEAELLQPFVESMALEV
                     SRALQFFFTSTQFNQINHIVLAGGCAVLPGADEVVASRTQINTIIANPFANMVLSDRV
                     RAKSLLVDASSLMAACGLALRRFDE"
     gene            20272..20838
                     /locus_tag="PLD53_00085"
     CDS             20272..20838
                     /locus_tag="PLD53_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRC59329.1"
                     /translation="MIRINLLPHREEKRKARRDQFYGLLGLVSVLAGLVVFLVYSIIA
                     GYITAQEAKNAFLKQEIAVLDKEIDQIKRLKEQTQALLARKQIIESLQQDRAEAVRLL
                     SELVKQMPEGVYVRSVKQVGVNISLVGYAQSSARVSTLMRNIEASPWLEKPRLIEIKA
                     SVVDKRRLNEFILNASLKRTPVDDGGKK"
     gene            20835..21509
                     /gene="pilO"
                     /locus_tag="PLD53_00090"
     CDS             20835..21509
                     /gene="pilO"
                     /locus_tag="PLD53_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRC59330.1"
                     /translation="MKAPSMAKIDINAFLDDFRNLNPKDVGAWPIAPRIAVLIGIFVA
                     ALVAGWWFVWNDQLETLETRQADELKLKDEFVAKKKQSVNLALYTQQLSEIDRSFGAL
                     LKQLPDKSEVESLLIEINQSGMGRGLQFELFKPGQEVSKDFYAELPINVRITGSYHDF
                     GAFAGDIGRLSRIVTLNNISITTDPKTKDGSLVMDAITKTFRYLDEEELAAKKKAAQA
                     AKGQKK"
     gene            21506..22027
                     /locus_tag="PLD53_00095"
     CDS             21506..22027
                     /locus_tag="PLD53_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HRC59331.1"
                     /translation="MKTIAVALASLALVACSGGDNEDLRQWMNEAAKDAKGSIPPLPQ
                     VKPYEPVPYDVGNLMDPFKPARIGPDQRKGGGGGLQPDMDRAREPLESFPLESLKYVG
                     VMSRKKTSYAIILVDGSLYQVRVGNYMGQNFGVITKILESELTLKELIQDSAGDWVER
                     ESSLLLQGQEGIQ"
     gene            22126..>23294
                     /locus_tag="PLD53_00100"
     CDS             22126..>23294
                     /locus_tag="PLD53_00100"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HRC59332.1"
                     /translation="MTVAQQGGVLNVKLVFKEPLNALPPGFSVAKPARIALDFANTVN
                     GLGKNSQTYNEGDLKSANIVQAEDRTRLVLNLNQAMTYESKIDGNSLLLTLVPSAKAS
                     GVPIVEHFSQTHPEQAANSIRDIAFRRGKEGEARITVDLSDPNAGIDIRQQGQNLVVD
                     FVKVSVPDALRKRLDVTDFATPVVSLNTIQQGANARMTITPRGLWEHNAYQTDNQFVI
                     EVKPVIENPNKLVQGSRGGYQGEKLSLNFQNVEVRRLLQVIGEFTGMNMVVSDSVGGS
                     ITLILKDVPWDQALDIILQQKGLDMRKNGNVILIAPREEIATKEKLEFESKAQIGDLE
                     PLRTESYQLNYQKAEAIQKLLTDPNQRMLSKRGSAVVDARTNILFVQDTPSRLEDV"
     assembly_gap    23295..23394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23395..23906
                     /locus_tag="PLD53_00105"
     CDS             <23395..23906
                     /locus_tag="PLD53_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HRC59333.1"
                     /translation="NAEISALESDGKGKVISSPRVMTANQVEAIIEQGVEIPYQQATS
                     SGATSVTFRKANLSLKVKPQITPDGKITMTLDINKDTPNTRLSTGAGVAIDTKHVKTE
                     VLVENGGTVVIGGIYTQEVRDNTQRIPFFGDLPYIGWLFKNREWIDDKTELLVFITPR
                     IVAESLAVR"
     gene            23991..24527
                     /locus_tag="PLD53_00110"
     CDS             23991..24527
                     /locus_tag="PLD53_00110"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRC59334.1"
                     /translation="MEKQYGTGNVYLVGLMGAGKTTVGRSLAKRLGLRFIDSDHEIEA
                     RTGVSIPTVFEIEGEEGFRKREAQVIADLSHLSDRVVATGGGVVLHPENRTNLRASGF
                     VVYLNVLPHTLWERTSHDRNRPLLQVANPLLKLEELYSQRDPLYREVADLVVDCSRTN
                     AHGVLQLLTREVGERWKH"
     gene            24515..25603
                     /gene="aroB"
                     /locus_tag="PLD53_00115"
     CDS             24515..25603
                     /gene="aroB"
                     /locus_tag="PLD53_00115"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRC59335.1"
                     /translation="METLTVALGSREYPILVGQGILENGGLLLPFLRSPKIAIVTNTT
                     VAPLYLERFSTPLRNAGIDIAEIILPDGEQHKNWQTLNTIFDVLLERRCERSSTLIGL
                     GGGVIGDMAGFAAACYQRGMPFIQVPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     KLVLADTDCLNTLPDRELLAGLAEVIKYGLIRDLPFLAWLETNLERLLEREPGALEYA
                     ICRSCANKAEVVVADEHEHGERALLNLGHTFGHAIETGMGYGKWLHGEAVAAGTMMAA
                     ELSRQLGWIDLDDLKRIENLFERAGLPVFGPAMSADRYLELMQHDKKVQDGKLRLVLF
                     KQIGQAVVSDMASPTEIGKAITARSTDV"
     assembly_gap    25619..25718
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25719..25976
                     /locus_tag="PLD53_00120"
     CDS             <25719..25976
                     /locus_tag="PLD53_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925801.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRC59336.1"
                     /translation="AQLEIKQFLRRHLYQHFRVLRMTGKAQRIIRDLFSAFTSDTRLL
                     PPQYQAAGDPFQIRRVADYIAGMTDRYAIKEHRRLFAVGDT"
     gene            complement(26010..26471)
                     /locus_tag="PLD53_00125"
     CDS             complement(26010..26471)
                     /locus_tag="PLD53_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC59337.1"
                     /translation="MEVREVLKVKNSAIFAAHPESLLSEAVIMMAENDIGSVVVMDDG
                     KLVGMLTFREVLQVLAKRQTERRSGPTPPIAEILVSEAMHIDPATTDPSMDVDELRRL
                     MIDTHSRYVPVMDGDTMTAVISFHDVAKAVLEERNFENKLLKAYIKDWPEE"
     gene            complement(26606..>26941)
                     /locus_tag="PLD53_00130"
     CDS             complement(26606..>26941)
                     /locus_tag="PLD53_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRC59338.1"
                     /translation="HMNVLLAEAKVPYDIVMEMDELNDDFPKTDVAMVIGANDIVNPG
                     AQDDPNSPIAGMPVLEAWKAKTSIVMKRSMASGYAGVDNPLFYKENNRMLFGDAKKML
                     DEVLVVLKG"
BASE COUNT         5949 a         7333 c         7783 g         5676 t
ORIGIN      
        1 gttagcccga acccggtcct cgacttcgag cacgacttca gcagcaaggt tgccctccgc
       61 ctgcatgatg gtcagtgccc gcaggtaggc ggggatatcg gccagcggcg tctccgccac
      121 ctgcaccggc aacacgtgcc agccgggcgt tggccatttg cggctggcga tcttcacctc
      181 ggtgcgcgtg tagtggcctg cagccaccgc ttccggcgag atcaggaaaa tgaacagctc
      241 gcagtcttca atggcgctgc ggatgcgatc gtcgaagctg gcccctgccc gcaagtcctc
      301 gcggtcgaaa aaaacctcgt gccccacggc ctgaagacga cagcacaggt cgtcggcgat
      361 ggcacgatac ttcgaagcat aggacaagaa aatcttcaaa ccggagcggc ctcacagacg
      421 gaagtaaaca gaatttatca ggctcagtat agtcaggtta tgacagaaca acccgcaagg
      481 ccagtgctgt gagaagggct cgaccagcat tctcggcagg ttggcgggtc gctcggcagc
      541 ccgtcgcagc ttatgatggg ggtcaatagt ggctgagggc tggccgcacc gggtgtaatc
      601 gaaacgtaat acgatcttcc tgttttggtg ctgctgtgct agcctccaca gcctgctttc
      661 atttcccgat ctctcccgtt tgcgatgagg ctgttttcct ttctttcgtt gcgccagtgg
      721 ctgacattgc cctacatcgc tctggtattc ggtgtcgccg tgctgatagg tgctctgtct
      781 taccgtaccg gcagccaggc cgtggatacg gtagcccatc atctgctgct ggaaacggtg
      841 gccaggatcg gacaggcggt tgaccggcat gtcgtcggct cagccgcggc gctcgaggcc
      901 gcttttccca acggcatgcc cgcaccagac acgattgacg ccgatctccc ggctttgcgc
      961 acccgtttct ggatagcgac ctcgctgcat atcgacccga acaattacgt ctactacgga
     1021 aaccgccacg gccagttctt tggtttgtgg cgcttcaatg tgcaagatgc cgaattgcgc
     1081 ctcaaactcc aagctgaccg acctcgccaa aaattccgct ttagcggcat taatggcgtg
     1141 ccgtccgaac cgaccacaga gcaaaaaatg tacgagccgc gtgcgcggcc ttggtacaag
     1201 tcgggcgaag ataatctctc gcacacctgg acgtcgatct acattgattt caccactgcc
     1261 gagttggtgg ctactcgagc aaggcgcgtg ctggatgcac aaggtggatt gcagggagtg
     1321 gtggcgacag acttgtcgct gaaacggctc aacgacttcg tccgtaaact cagcctgagc
     1381 gacaacgcgg tagccttcat catcgagccg gacggcaagt tgattgcttc gtcgcgcagc
     1441 gccaacacga tgccccatcc ggatggcact aatgcccgca tcaatgcgtc cgacagcgac
     1501 aacgaacttc aacgcgcggc ctatgcgcag gtgctcggct ttgccgctgc gggcaacaag
     1561 atcgaccgtg cgctgaccca gcgctttgcc gggccgaacg atcaggtggt ggaacttgcc
     1621 ctggaccgtg tgcgagacga tgccgggctg gactggatta ttgtggttgc ggtgccacgc
     1681 agcgatttca tgcagggtgt tacggccaac gtgcagcgca cagtggtgat tggcgtgttc
     1741 ggcgcgcttg cggcggtggc cattggcctg agcatcctgg gctgggtcgg ccgcgatatc
     1801 agacggctca cgcaggccgc gcaagacgtg ggtgagggac ggctggaggt gccgctggat
     1861 gtgcaccgca acgacgaaat aggcgtgctg gccaacacat tccgtcatat gcagcatcgg
     1921 ctgcgaacgg atttgctgac cggtctcggc aatcgggacg tgatgatccg cagcatcagc
     1981 agccgcatcg agaaagcccg tcgaacgggc gattcgatgc cgtttgcagt gctgttcgtc
     2041 gacctgaaca atttcaagct gatcaacgac cgatttggtc acgatgcagg cgaccgcgtg
     2101 ctgatggaaa tcggcaatcg cctgctcaag gcgtcgcgcg cctgtgatcg ggtggctcgc
     2161 tatgcagggg acgaattcct gctgatgatc aatgacattc catcgcggca ggttgcagag
     2221 cagataaggc acaacatcga ggagttgctg cgcgaacccc tgcaaacggt agacgtatct
     2281 tcactccccg aagccgtgct gatgagtggc tcggtaggca tggctttcta tcccgacgat
     2341 ggggacaatg ccgacgagtt gatcaagcgt agcgatcacg acatgttcga acggaaacgc
     2401 acctggcaat cggactgtat ttgaggcgcg tgtccaagcg ccagcaggct acttggcgac
     2461 ggtagcttcc gcgatacctg ccagagcttt gctgagattt tcaacgaccg gacaggctgc
     2521 aacatcgtgc cgcttggcca ggaaagccgg ggcgttgtag gagagccaaa cctgggatga
     2581 ggcatcttcc cagaccagcg ccttgagcgg caggtcgata ccggcggact gcgcgcattc
     2641 catgaacggc gtcccaccct gggggttgcc gaagatcagc aattcagtgg gacggagtga
     2701 tttgcctatc gatgcggcgc cagcggcatg gtcgatacgt gcgaagactt tcaggccacg
     2761 ttttttcacc agctcttcca gtcgattcat cgtctctttg gcgctgtatg ggctcttgac
     2821 ggaaaccagt ccgtcggcgg ccgaggccac cgggacaagc aacgcgagcg tcaatgcaac
     2881 gacaagtttg acaatcttca tggcggtctc cttgtgaggc agggtaaagc ttcagtatgg
     2941 cgcttattgg gtcatcttca agatactgtt cgaatcagga ctcgacaaca gcaatcttta
     3001 cgttcaagca gcgggtcaga accgggcaaa gccatcaggc attgaccggc gcgctacggc
     3061 tgcggatact tggaccccat ggcatgtctc gactcgggca gccaatcaga tgccttaacc
     3121 ccactgaacc aattccaaga tgccttcatg ctgaaaaccc tcaaagacct gtttgactcg
     3181 ttcaccggtg attcccccga ctcctcgccg cgggcgcgcg aacatgccct gcaactcgcc
     3241 accgctgttc tgctggtgga agtcatgcgg gcggacccgg ccatcgaagc ggccgaacgc
     3301 acagccgtac tggcggcgct gaaggcaaaa tttgcgctgg cggacgacga gtcggaccgc
     3361 ctgcttgaag tcgcggagca aaccgccaaa gacgcctatg actaccaccg ctttaccaac
     3421 agcatcaatg ccagcttcac acacgaggag aaagtgcgcg tgatcgagag tatgtggcag
     3481 gtcgcctacg ctgatggcca tctggacagc cacgagaacc acttgatcag caaggtggct
     3541 tctttattgc acgtgacgca ccgtgaatac atcgccgcca agctgcgcgc caagtgctag
     3601 ccgccctgtg acactttccg cgtttgccgt caaatgaagg ccagatacca caggatgctg
     3661 gccgcgattg ccctctatgc ggccggcgag acgttgccct ccgggtcggt aaagagcacg
     3721 cgatcgcctt caatcgaaaa ggatcccgtt tcgtaggtct tgcgcaatcg cgatactttc
     3781 ttgccaaaca gtatatcgac ggcgcccacc acgttcaaac cgatttcaac ccgcgcaccg
     3841 gcgattagcg ccagttgccg ttcgagttcg tcgcgttgcg gcaagagttg ggcccaggct
     3901 ttgatcgcct gctcccagga cgctttgacg cgttccagca tgccgttctt gtcgccgatc
     3961 ttggtcaggt gcttgaccag catctcaagc ttgctttcct cttcccgctg cttctcgatg
     4021 accttcaaca ggtcctgata ttgcgcttca agcaccggat taacgccaag aacgagattg
     4081 gttacgcccc cagtctgcga tccaagaatc ggtgttttta tcaacatcat tgcctttgcc
     4141 gatccaccgg ccagacgtcc ccgctgcggc gaactgacgc caacaacaat ctggttgttg
     4201 gcctgcagct cactttgcag agccgaatca tcgatagcga tgatggtccc ggagaataca
     4261 tgcgcgcttt cgacaaagcg cgcggaaacg gaaccccctg catgcacgcg agcctgggaa
     4321 attattccgc cagcgacgcg gatgtcgcct ccagcatcca gctcggcgcc atcaaccacc
     4381 tcaccaacaa cgatatcgcc ggacgcatgc actttcatac ccggtgacac atcgccctcg
     4441 atgtttacgg ttccgtcaaa gcttatattt ccagaagcca tgttgacatg gctaatgttc
     4501 aaaacctgct cgacgttcac gccattgcca gcgcgcaccg gctgaccgct gaacactgcc
     4561 cgtaacaggt tggcatcttc tttgtcgaca taggcgccga tcagtccttc gtcaaacagc
     4621 gcatcctgac ctggcacggg ttcgatgact tcgccacgaa cattgcgccc cgctgttccg
     4681 gtcgtcggag gaatgcgtcg catcagtggt tgttcggcgg ccaccattgg aatcgctccc
     4741 agttcgcgga agtcaatcaa gccgttctcg ctcacctgcg gcgtacgatc gcgcgtatca
     4801 gccaccaaca tctcgaaatg agcgtctata ccgtcttctg ccggttgccc ggctgccacc
     4861 agtattcgct cggccgaatc tgacgcgcag gccgcactca ccgccgacga atctatacca
     4921 acgacaactc cggtttcctc gagagcctga tatacgtcgt tcggatcgag tgctattcca
     4981 ccataggagg gaatgaaatc cgcccatacc tgcatggcat cctgcgccat ctcaagcttg
     5041 aaactcgcat cgctgcgttc accaatcggc aatttcaatt cgctcgtcga tgaattgaag
     5101 tgctcgatca gtgcctgcaa cgccccttct gaaagatgcc agcttccaaa acccgcttcc
     5161 gccagcagtg ctcggaggga atccggttcg agaaatgcac gtgcttcgga agcgctgata
     5221 tacgcaatga gacggttgtc atccaccgac aaggtcaaac cggagaattc agtcatcgct
     5281 cccccttttg actacgacac atgacacgat tgacaagtgc caagcgcaac ctatccgaaa
     5341 gaacgtattc tgacaatcac aagcttgccc cgctgttgcg cgtgacgtac cgcgaatgcg
     5401 tcgccgccaa gcggcacgtc aagacccggc cggcctgcaa cgctttccac gtttgccgtc
     5461 aaatgaaggc cagataccgc agattgccgg ccgccacagc ggtctttcca gtccgatctg
     5521 cgatttttag gggtggatta ttccgccaga ggcaaggcca ggcaaagccg ggtgccaccg
     5581 agtggcgaag gcagcggctg caggctgccg ccgtgcagcg ccgctacgcg ttgtgcgatg
     5641 gccaggccca gcccaccgat gccgccgctg gtgcgtttga ccggcgggtc gcgcaatgac
     5701 tttccctgtt cgaggcggct gaacaattcg tccggcagcc cgggtccgtt gtcctcgatc
     5761 agaatattgg cctgcgcgcc gtcccggctg acactgacgt gaatcggtga agtggtcggg
     5821 gcgtgccgca tggcgttgtc gatcagattg gtcagcgccc gctcgatgag ctgcaagtcg
     5881 ccttcgaatt ccagaggtcc ggggggcgca ccgcgcagag tgacgggcgc tggcccatcg
     5941 gccagatcgt acttctgcac ggcatccgtc accagctcgt cgagtctgaa acgctcgcgg
     6001 tgcagcacct gatcggtgga ttgcagggca gcgagttcga agagctgctg cgacagtcga
     6061 cgcactttgt cactctgcgc caatgcagca gcaagtacgc gatcgcgctg ctccggttcg
     6121 acgagcgcct gtcctgccat cgcttcgagg tggccatgaa gcgccgtgag cggggtccgc
     6181 atatcatgcg caacgctggc catcacctcg cgatgcgcac tggcctgttg ctgttcgcga
     6241 gcagtctgcg cctcgatacg ctgcgtcatc tcgtcaaacg cagaaccgat cgcgctgacc
     6301 tcgtcaccgt tagttcgaga ttggctgcgt ccagccgcag ttgcctgcag ggtgcgcacg
     6361 ctgtagtcgc gcatgcgatt ggccagacgg tgcaggggca aggtcagccg gtgcatggcg
     6421 aaaaatccga gcaacagcgt caccaacaag ccagctgccg cgacgagtcc tgccccctgc
     6481 cagacccggc gcaggctcaa ctgtccggcg acatgaacgc gggcctgtcc gtcaagcacg
     6541 acgtacaggt atccgggcgg acgcacatcg ccggcacgcg ggggaaacat cgccgcactg
     6601 aagatgcgcg gtatgcctgt gcccatcggg tcggtaccgc gcaatggcaa ggcagcgcca
     6661 gccagaaagc tgcgtaccgc gccgaggtca acctgtttct cacgcaccat gccgggatcg
     6721 ccgatgtagg cctgaacacg accgtcagca tcaagcacat agacctggat tcccggattg
     6781 accaccatca acatggacag cagcgactcc cgtgcggcgc gatccgcttg gtcagggtcg
     6841 gccacggcaa tttccggcca gtgttcaacg atgtgcttgg ccagcccatg cgacagccgc
     6901 tgtagtgatt cgtgctcata ctccacggca acatgccgcc caagaagacc aacgagagca
     6961 ccatatgcaa gcagtagcag cgatagcgcc agcgtcagac gggtggtgaa cgagcggccg
     7021 ccgatcatga cggattcgcg tcgaagcggt agccaacgcc ccacacagtg acgatgcggt
     7081 gcgggtcacg tgggttgtcc tcgatcttgg tgcgcaacct gttgatgtgc gagttcacgg
     7141 tgtgctcgta gccatcgaaa gcggcacccc acacgcgttg caataactcg ttgcggctga
     7201 aagcgcgtcc tgggtggcgc accagaaaat gcagcaggtc gaattcgcgc aacgtcagtg
     7261 gcacggtatc gttaccgcgc aggagctcgc gccgcaacgt gtcaagccgg aagggaccga
     7321 agccgacctc ggatgcagca gccggcgacg aacgcaactg ttcgatacgt cgcaacaggg
     7381 cgcgcacgcg cgcgaccagt tccagcatcg agaacggttt ggccaagtag tcgtcagcgc
     7441 caagttccag gcccagcaca cgatgtgcct cggccgagcg ggcgctgagc ataatcaccg
     7501 gcacatctgc gtggcgcgcg cgcaggtgac ggcagacctc ccagccgtcg gcgccaggca
     7561 gcatcaagtc gagcagaacc atgtcccagc gttggcgatc aatcgccgcc atcgcgcgca
     7621 gtccgtcgga ttcgcgatgc aggcgatagc cggcttcctc cagatgaagt cgcagagcgc
     7681 cggaaatggc atcatcgtcc tcgacgagca gcagttgttc gttcaccgcg aaaccggcac
     7741 cacacggtag tggcaccgga gcactgcagg agtcgaagat ccggcgctcg acttcacacc
     7801 aattcgctac ccgattcgaa cgactgcccg cggcgacggc gtgagatcca agcgacggcc
     7861 aataagccag ccaccatcaa agcaatggtt tccggctcgg gaacagcctg gaacgtaata
     7921 cgatagacgt cagtatcggc ggtcagcgcg ctgttgaaca cgtagcctgt agccgtggtc
     7981 aggccgtcga acccggcaag ttcggcgaaa ttgaagctga cgtcgccgtt ctggtcggtg
     8041 cgcaagtcat tgttgccgac gaaggctgcg gcagccgggt caaacagctc ggatccggca
     8101 tcccagatat cactggcttt ctgcacgatc gatccaatga ccagggtgcc aaaggcgtcg
     8161 aacaactgat actgcgttgg cgaatcgttg ccgatgaaaa aatcgttgct tggcacgacc
     8221 attgaggcga aggtgaagaa acggttaagc gcagtatcta ccagaaatga aaagctgctg
     8281 gtctgcccag gttgcagcag tccgccaatc gtgccccggg tcgccgacgg atcggccgcc
     8341 gcaaacgccg gttgccaagc cgagccactg ccgccttccg ccaccgagat gatggctgct
     8401 gtggccgcct gaccgttgtt gaaggagtcg aaagtgccgt tgttgaaacc gacgtgcagt
     8461 ggggcgaaac tgatgctgtt ggcgggtgcg aggttttcga ccttgacgag gatattgagc
     8521 tgagccgcac tggttgtggc gctaagcacg agcgccgcaa tgccagcggc ccgccgtaga
     8581 gaagtggaag aaataaggtt catggggctc tcctggtgaa aagtatcagg ttgggaatcg
     8641 agcccgaagc aatgagctcg catgaacgat gatagaaagc caccatcccc gagtcctcat
     8701 cgaaacgtca cggcgccgtg atattttcgc ttggcgggcc gacgttcacg caaaccagtg
     8761 caatacaccg aaaggacatt gaatcgccac atacggagta tctgtggccc tttcagcgct
     8821 gtcaaatgga tcagcaaaaa atcatcgcgg tacaatcgac cgggaccaga cttttaatct
     8881 cttgcaacat ggcggtgaaa atgatcccgc tgctgcagtc gcgacgctca cgtcctggcc
     8941 ccggtcggca caatcccctc ccagggctca aagaaaacgg ccttcgtcca gcgagacaag
     9001 aaacaactga ggattacata tgcacatgga ccccttcatg ccagaactgg ttggcagcat
     9061 cgtggccatt ctcctggctg gcctgctgat gcacgaactg cgtcagccca atgtaatcgc
     9121 ttacctgatt gccggcatca tgcttgggcc gtcgctattc ggggcgatta ccgatcagca
     9181 ggtgctggac cggcttggtc aattcggcgt cgtgatgctg atgttcttca tcggcatgga
     9241 ggtctcgccg gaaaaggtga tcgccaggtg gcgggtggcc ctgcttggca cattcatgca
     9301 gatcgtcgtc agcgtcgccc tgaccaccgc catcggtcac ttttttgact ggccgttcgt
     9361 gctgtgcgtt ttgctcggct tcatcatctc gttaagcagc acggcggtcg tgctgaatct
     9421 gctgcaagcc cgcaaggagc ttgaaacaag gcacgggcag aatgccttga acgtacttct
     9481 ggcccaggat attgccgtca ttcccatgct gatcgttgtc agcatcctca gtggcgagaa
     9541 agtgtcgagg atggaaatga ccatgcaggt catcggcgga atattgctga tcgcttgttg
     9601 cgcgtggatt gtgatccggc ctggtttccg cattcccctg atcggcgacg cggtcaagaa
     9661 ggatcacgaa ctgcaggtgt tcggtgcgat gttgctctgc ttcggactgg cattgctgac
     9721 cggactcttt tcgctttctt cggctttggg ttccttcgtc gccggcatcg tggttgcggc
     9781 agccaaggag gcgaagtggg tgcatcatag tcttgagcca ttcaaaacag tgtttgtcgc
     9841 actgttcttt gtttccgtcg gcatgttggt ggatatcgat ttcgttcttc accactgggg
     9901 caaagtcctc tctctggcgc tgattgcgct gacggtaaac atggcgataa atgcaacggt
     9961 attcaagata ctcggcgaga cttggggaga cagtctctac actggcgcaa ttctggccca
    10021 gatcggtgaa ttcagttttg tgctggctgc ggtgggtgcg tcttccatga tcatttcgaa
    10081 ggaaaactat cagcttgcgg tcgcggtaat cgtggtctct ctgctgctgt cgccgctgct
    10141 gatttcacgg atgcgccgtt atgcgcttgg cggagatgaa ttgcacaaag agccgtgagc
    10201 cggcgcatcg acaatcgtca cttcagtact cttcgttgaa tcgcctgatc tgccgtcgtg
    10261 ccttcttgct cgggcgaccg cgcagatcac taccgggggt gggggcgagc cgtttggatt
    10321 ccctctcggc gttgcggtgt gcggcacttt ccggtgtctc agcatagagc tgctgtgctt
    10381 cgctggccgg acggcgctga tcgttcaagc cgcaaaccgt gatcgtccag agtgtatcgc
    10441 caacggtaat accgagcgtg tctccaatgc gcaattcgcg cgccggtttg acgccgcacc
    10501 cgttgagttt gacgtggccg gaatgaacgg cttccgtggc cattgagcgt gtcttgtaga
    10561 agcgcgcggc ccacagccat ttgtcgatac gcaggcttgg gagcggcagt ctttcaggtg
    10621 caggcatagt gttggaagcc cgaattcatc gagtttcagg cattgcgggc aaatctgtgc
    10681 ccttgggcgt gaatcggtca aggtagacat agatcaccgg cgtcaggtaa agggtgagga
    10741 attgcgaaag cagcagaccg ccgacgacgg caaggcccag aggtcgacgc acatcggcac
    10801 cggcgccgaa gccgatggca atgggtagcg tgccaaccag cgcggccatg gtcgtcatca
    10861 tgatcggtcg aaagcggatc aggcaggcat tgaatattgt ctcatgcgct ggaagttgcg
    10921 aactgcgctg ctgatcgagg gcgaagtcga tcatcatgat ggcgtttttt ttgacaatgc
    10981 cgatcagcat gatgatgccg acaaacgcat aaaggctgag atcgacgcgg aaaaccatga
    11041 gcgtaatcag tgcgccaagt gcggcagaag gcagcccgga gagtatggtt agcgggtgga
    11101 tgaagctctc gtaaaggatg ccgaggacga tatagacgac cagaatggcg atcaacagaa
    11161 gaatacccag cccctgcaac gaagcctgaa aagcctgagc ggtgccctgc aggctggtgt
    11221 tgagcgatgc cgggatacgc aattcctgtt cgagcgcctt gatacgcgta accgcctcac
    11281 ccagagagat gccgggcaga aggttgaagg acagggtgac tgcgggcagt tgcccctgat
    11341 gattgaccgt cagggcttgt gtcttgcgac tgataccggc gatggtatcg agtgggatca
    11401 acttgccgct tgaagcgcga acgtgcagac gggagagcat cgatgggtcg ctctggaatt
    11461 cgggcgcgac ctcgagaatg acctgatact gattggcggt gccataaatg gttgaaatct
    11521 gcctcgcact gaaggcgctc tgcaaggcat cctcgacctg actgaaggtg agaccgagtg
    11581 ttgtgatttt gtcgcggtcg atatcaagcg agacgaccgg gctgtggttg ttgaggttgc
    11641 tggtcacgtc gacaaaaccc ggcaactgac gaatccggcc gagcagtgtt tccgtccatt
    11701 ggtagagttc gtgcagatcc gtgctttgca gtgtgtattg gtattgcgcg gctgtgatca
    11761 ggccaccgat gcggatgacc ggtgggttct gcatatagac cttgatgccc ggcacggtcg
    11821 ccagcctggg tcgaagttgc tcaatcagct cgtccggaga gagtgtgcgc tcaccactcg
    11881 gtttgagaat catgaagatg gtgccggtgt tggacgtcgg tcggatgccg ccaccgccaa
    11941 tcgacgacat gaatgactgg atattcggat cctcggcaac gattgcggcg acagcgcgct
    12001 gatgctcgac catcgaggca aaagaagcat cttgcgcgcc ttcggtaaag gcaatgattc
    12061 gcccgctgtc gccgctgggg atgaaatcct tgggtaccat cgagaacagc aggccactca
    12121 aaccagcggt ggccacaaac ccggcaacaa ccagccgagg gtgcgctatc gcctggcgca
    12181 gagatttctc gtagccggcg agcagcgctg cgaagaattg ctcgaaggca cggaaaaggc
    12241 ggccgtgatc ctcggttctc gatggcttta gataacgact gcacatcatg ggcgtgaggg
    12301 taagcgaaac cactccggat acgagaatgg cagcgcaaat ggtgacggca aattcgtgca
    12361 acaaccgacc gacgatgccg cccatgaaca gcaccggaat gaacacggcg accagtgaga
    12421 cggtcatcga aatgatggta aagccaatct cccgcgaacc cttgatggcg gcgtgcatcg
    12481 gagagtcacc cagctcgatg tggcgaacga tgttttcgag catgactatg gcgtcgtcaa
    12541 cgacaaagcc gaccgacagg gtcagcgcca gcaaggagag gttatcgaga ctgaatccga
    12601 gcatggacat cacggcgaac gtgccgatca ccgagatggg caacgccagc gcggggatga
    12661 gcgttgcgta gagattgcgc agaaagagca ggatcacgag gatgaccaga aaacccgcga
    12721 gaatcagggt gaactggaca tcttcgatgg catggcgaat cgagacgctt cgatcataga
    12781 gggtggtcat gctgattgcc gccggcagct ttgcctgaaa actgggcagg atgcgcttga
    12841 tcgaatcgac ggtttcgatg gtattggcgc ctggctgacg ctgaatggcg aggacaatgg
    12901 cgcgcttgtc gacgttccag ctggcaaggc gcaggttttc gacactgtca atcggcgtgg
    12961 caacttcttc cagtcgaacc ggcgcaccgt tgcgccaggc gacaatgagc ggtcggtaag
    13021 cgacggagtt atcgagttgc ccgttgtcct ttatggcaaa cgtgcggcgt tcgccgtcga
    13081 taagcccaac gggttgattg acgttcgcct gtgctacggc ctgatgaagt tcatcgatac
    13141 ccagaccccg tgcggcgagc tggtcggggt ttgcctggat tcttacggca aatttctgcg
    13201 aaccgtaaat cagtacttgc gcaacgccgc tgatcgttga tatgcgctgg gcgagttgcg
    13261 tctcggcata ttcgttgacc agcggcagtg gtagcgtcgg cgaggccagt gcaatgtagt
    13321 aaatgggtga atcggccgga ttgaccttcc ggaacgaggg cggtgccggc atgttcggcg
    13381 gcaatttgcg caacgcgacg gcaatggcgg actggacatc ctgggccgcg gcgtcaatat
    13441 tgcgatcaag cgtgaactgc agggtgattg aggtgctgcc ctgagcgctg gttgaattca
    13501 tcgaatcgag gccggcaatg gtcgagaact ggccttccag cggcgtggca acagcggcgg
    13561 ccatcgtttc cggcgatgca ccggggagtg cggcagtgac ggagatggtc ggaaaatcga
    13621 cactgggcag ctcggaaacc ggcagtgcgc ggtaggcaat gatgccgaaa atgacgaacg
    13681 ctgccatcag cagcgtggtc atgaccgggc gacggataca gagttcggga agattcacgc
    13741 ggcaaccgcc tagtgatttg ccggcttgcc cgaagcttca gccgagccag gttcgtcagc
    13801 atacctgacg atgactctcg ttccaggcgc cagccgtaac tggccatcgg tcacgaccgt
    13861 ctcgccgacg ttcaggcctt taccgattgc cgtcatgccg ccatccgaag ctgcggtttc
    13921 gacctgacga acctcgacgc ttccatcatc cttgacaaca aagacaacgt tgccgcttgc
    13981 gccctgctgg atcgcttcgt cgggtacggc aacgaccttc gttagcgtct ggagcagcag
    14041 tgtgacattg aggaattgcc ccggagtgag cgtttcatcg tcattgggca attcagcctt
    14101 catctggatg gtgccggtag cgctatcgac ggtgttgtca aggaagcgca ctcttcccac
    14161 gaagcgttgc gaatcgtttt gcggcacgtt gacttcaatc ctcaggccgt caagtgttgc
    14221 cgtgccgtga cccagcgccg ctctcagttg aggcaggtat ttctcgggca cggagaagct
    14281 gaccagcaag ggccgtacgc ggttaacgac ggccagggtc gtgtcgttga tcttgaccga
    14341 cgatccagga aagaccagcc gagcgccgac tacaccatcg aacggagcgc gaatggtggc
    14401 gtaggagagc tgcagacgcg ccaattccgc cgcggctttg ctggcgcgca ggctggcagc
    14461 cgcggccgat tcattggtgc ggatttcgtt gaccttctct gcggagacaa aattgcgatc
    14521 cttaagcgcc atgtagcgct gggtatccgc gcgtgtcttg gcggccaggg attcgtcccg
    14581 ggcaacgctt gcctcagcct gcctgagccg ggcggaaaaa tcgctgggat caagccggat
    14641 caagatatca ccctgcttga cgtgttggcc ttcggtgaac aacacctcgg caacctgacc
    14701 atcgatgcgt gatttcagga caacgctcgc aaaggcttcg gcccgaccaa cgactcgtaa
    14761 acctaccgga atatcctgtt cagtcgcctt gacggcgata acgggaacgg gcggcggcgg
    14821 ttttttctcc gtatcgcctt ccgaacatgc agtcagaatc accaggccgg cggaaagaaa
    14881 ggcgtaccgg aaggtgatac ggacggtttt cacggtaaca atgtttcgtg agcgtaaagc
    14941 tcttcaacag tctcgcgcct gcgtatcagg tggacttctg taccatcaac catgacctcg
    15001 gcggcccgcg gccgggtgtt gtagttcgaa ctcatgacca tgccgtaggc gccggcagac
    15061 atgacggcga gcaagtcgcc gggctcgatg gccagcgacc ggttacgacc gagaaaatcg
    15121 cccgattcac aaattgggcc gacgatgtcc cagtcgcggg gagcgacact gcgtggtgat
    15181 accgcaacga tgtcatgcca ggcatcgtag agtgccggtc gggcaaggtc attcatcgcg
    15241 gcgtcgacaa tggcaaaatt cttcgcttcg cctggtttga ggtattcaac ccgcgtgagc
    15301 agaactccgg cgtttcccac cagacgacgt ccgggctcga agagtatctt gaggctccga
    15361 tccttgagtg cttgcagcaa gggccgcaga tagtccttga cggtgggcgc ttcttcgtcc
    15421 ttgtaacgga tgccgaggcc cccgccgagg tcgatatggt gcaggtgaat gcctctggcg
    15481 gcgagctgat caatgagcag taagagcttg tcgagtgctt cggcaaaggg ggccgggtcg
    15541 agcaactgtg acccgatatg gcagtcgata ccggtgattt caaggttcgg caaacaggca
    15601 gcgtgctggt aaacctccag ggcgtcttcg tacgcaacgc caaacttgtt ctccttcagg
    15661 ccggtcgaaa tgtaaggatg cgttttggca tcgacatccg gattgacgcg caggctgatg
    15721 ggcgccttct tctttagacc gccagccact tcattgagtc ggtcaagctc gggcgccgat
    15781 tcgacgttga agcagagaat gccggtatcg agcgcatagg ccatttcggg ggctgatttg
    15841 ccgacgccag agaagacaat ctttttcgga tcggcaccgg ccgcgagagc acgctgcaat
    15901 tcgccaatgg aaacaatgtc gaatccggcg ccacgacgcg cgaagatgtt gagtacggcc
    15961 aggctggaat tggctttgag cgcgtagcag accagcgagt caataccagt caactcctgc
    16021 tggaactcat cgagcgcggc ttcaagtgca gcgcgggagt acacgtagca cggagtgcca
    16081 aagcgcgctg caatgtcggc aagcggaacg gattcggcca gcaattgccc attgctcagg
    16141 gaaaaggcat ccatcaaacg tgttcttttg ccgtgctaag atcggcatgg gtcagcgatg
    16201 gcgtgatctg caacgataac gcggaaacgc gtgctggcgt atcgggagca taggattgcg
    16261 gcggcggtag atacagcgat cctcgcgtac cgcacgcgcc aagagcggac gtaaccagca
    16321 gaacggtgag cgtgagaaat cggcgcatgt ttgtagtacc cgcaaatgga aaatggtagc
    16381 acagatgaat gatagcgaat tcaatgcact tgccgatttg gcgctgacac gaatcgagaa
    16441 cggacttgag tcgagcggcg ctgatctcga ttttgcaatg gtcagtgccg gcgtattcga
    16501 aatcgaattt gccgacggta gcaagatcat cgtcaaccgc catggcgcgg ctcaagaact
    16561 ctgggttgct gcccgctcgg gaggctttca ctatcgatgg gacggccaaa actggcgaga
    16621 tacacgcgac ggtaccgacc tcatggctac gctagcgaaa ctggtatcag cacaggccgg
    16681 cgaacctgtc cagctgaatt agccccttgg atcaatcgat cggcacatcg tctggatcct
    16741 ggggcggttc gatttcgatc actgggggaa cgttttcctt gtagaaaaat tccctggccg
    16801 ggcctttgcc cgggttttct atttcgagcg caaccaatcc atcaggttga gacatgaagg
    16861 tctggggcac atctttgagc gcaacggcca tatagccgat ccaggtcggc agcgctgccg
    16921 agccgccggt ctcacctttt cccagtgtcc tgggctgatc aaaaccgatc catgaacaac
    16981 cgacaacggt ttgttgatag ccgcaaaacc aggcatcgac gtattcattg gtcgtcccag
    17041 tcttgcccgc caaatcacga cgtttgagtg tggccgacgc gcgcgcgccc gtaccataga
    17101 tggtcacgtc tcgtagcatg ctgtccatca gataggcatt gcgtgggtcg atggctctta
    17161 acgattcatc gccggcggtg atcggttgtg cctcggccag cacctgcccc ttatcatcgc
    17221 ggatatcacg cacgataaaa ggttggatcc gataacctcc attggcgaaa acagagtagg
    17281 cggcaaccat ctgccagggg gtggccgcac cagcaccaag ggccatggtc aggtagggag
    17341 ggtgcttgtc ggcatcgaat ccaaatcgcg tggcgtagtc ttgaatgtaa ttgactccgc
    17401 tggcttgcag aaggcgaatc gaaaccatat tcttggattt ggccagtgcc gtacgcatcc
    17461 gcatcggccc ggcgtatttg ccgtcgtagt tcttcggttc ccaggcctgg ctacccgtga
    17521 ccgaggcggg gatgctgatc ggtgcgtctt caatgatcga tgccggagta aagccctttt
    17581 ctagtgcacc cgaataaatg aatggcttga aactcgatcc cggctgccgc caggcctggg
    17641 tagcatggtt gaacttgttg cggttgtagt caaaaccacc caccaaagca cgtattgcac
    17701 catttcgagg gtctgtagcc aggaaggcgg cctcaacctc cggcatctga gtgattcgcc
    17761 aattgccgtt gtcatccttc tggatacgaa ttactgcacc acgtcgaagt cgtttgttcg
    17821 gcggcgcctt gtcgtccatc atgcggaggg caaacttgag gccgtcgccg ccaatggtaa
    17881 cgatctcacc gccgcgtcgg taggctcgga tctgcttggc atcagcttgc agaacaacgg
    17941 ccggatgcaa atcctccgaa tcgtggaagt cctgaagcac ctcgtccagt gcttcatcct
    18001 gttcggactt gatgcccgac atgtcaacat aggcttcggc accacgatag ccatgacgac
    18061 gatcataatc aaacacaccc cgccgcaagc tgcgataggc ggcttcctga tcgccattga
    18121 ggatcgtcgt atagacgctt agaccgcgcg agtagacatc gtcagggaaa cgctcggcag
    18181 ccaactgccg cgccatttca gcgacaaatt cggcgtgaac ggcatataca gtggtatcgc
    18241 gcttgacgac cagcgcctgt ttcatggcgg cttcgtgttg cttgggatcg atgaatccca
    18301 gttcgagcat acgtcgtaat acatattgct gacgcaaacg ggcacgcttg gggttgacca
    18361 ccgggttgaa cgccgatggc gctttaggta gtccggccag catggcggct tcggcgattg
    18421 acagatcagc caaaggcttg ccaaagtaga tttgggccgc ggcgccgaag ccataggcgc
    18481 gctgcccaag aaaaatctga ttgatataca actcgaagat ctgatccttg ctcaagctgt
    18541 tttctatctt gaaggaaagc aacgcttcat acagcttgcg ggtgtaagtt ttctccgatg
    18601 acaggaagaa gttcttggca acctgttgcg taatggtcga agcgccctgc cgtttgcccc
    18661 cgccggtcaa attgctcaat gcggcacgtg caatgcccag aacgtctaca ccgcgatgct
    18721 ggtagaagcg ctcatcctcg gcggccagaa tggcctgctt catcacgtca gggacgtcct
    18781 gaaaccgtac aacctcacgg tgttcttcgc cgaactcgcc aatcagctga ccttcagcgg
    18841 tgtaaacacg tagaggaatt ttcggccggt agtcagtcag gatttcaagc gaaggcaggt
    18901 ttgggtaggt cagaatcaga actacggcgg ccatggcgat gaccatcgct gcaaggccag
    18961 caatgaaaag gacaggatag atgatccagc gggaaacgtt aggcaaggtc ggggccggga
    19021 gtaaagacgt agcgcgacat tataaacgtt gcctcaagta ctgtggcaga gagcaaaaat
    19081 agcgaaaaaa aatggcttta cacgccggat acttgttgct agcatctaaa gtagattatc
    19141 tgtaaacacg ctaactacaa attattcaag ggttttttcg gcggggggtg gcaatttgca
    19201 actcgatcta tcgatcttta atcctaaggc gcgctccctg atcggtcttg atatcagctc
    19261 atcggctgtc aagatggttg aactcgcgag cgatggcaag agcgggtacc gcgttgagcg
    19321 gtatacaatt gaagtgctcc caagagatgc tgtcgctgac ggcaatattg tcaatctcga
    19381 agcagctgca gagactgtgc gtcgggcttg gaagaagctt gcgacctcga ccaggcatgt
    19441 cgcgattgcg ctcccggctt cacatgtgat taccaagaag atcattgttg ccgccggtca
    19501 gcgtgaggaa gagctggggg ttcaggttga atccgaggct aaccagtaca ttccgtttgc
    19561 ccttgaagaa gtaaacctcg actttcaggt tgtcggcccg gcagcgtctt ccccggatga
    19621 aatcgaggtt ctgattgccg cgtcacgcaa ggagaaagtt gaggatcgag ttgccgtagc
    19681 ggagtctgcc ggtctaaaac cggtggtcat ggacgtcgag tcctatgccg tgctttccgc
    19741 ttttgagttg atcgaaaagc aactccctga cggtggtaac gggcaaatcg ttgcgttggt
    19801 cgacgttgga gccaacgtga tgaacttgac cgtactacgc aacggccagc aactctatgt
    19861 tcgcgaacag gcgtttggcg gaaatcagct gacacaggac attgcccgcc tttttggcat
    19921 gaactttgag gaggccgagg ctgagaagcg gcggaacaat ctaccggaca actacgaagc
    19981 cgaactcctt cagccgttcg tggagagcat ggctttggag gtgtcgcgtg cattacagtt
    20041 cttcttcacg tcgacccaat ttaatcagat caatcacatt gtgcttgctg gcggatgtgc
    20101 tgtcctgccg ggggcagacg aagtggtagc gtcccggacg caaatcaaca caattattgc
    20161 caaccccttc gccaatatgg ttttgagcga tcgggtgcgc gccaagagcc tgctggtgga
    20221 cgcatcgtcg ctgatggctg cgtgcggtct cgctctgcgg aggtttgacg aatgatacgt
    20281 atcaatctcc tcccgcatcg ggaagaaaag cgaaaggcac gacgcgatca gttttatggg
    20341 ctactgggct tggtttcagt ccttgccggt ttggtcgtct ttctcgtcta ttcgattatc
    20401 gcaggctaca tcactgcaca agaagccaag aacgcgttcc ttaagcagga gatcgccgtt
    20461 cttgataaag aaatcgatca gatcaaacgc ctgaaagaac aaacacaggc cttgctcgcg
    20521 cgcaagcaaa tcattgagtc cttgcagcaa gaccgagctg aggccgtgcg cctgttaagt
    20581 gaacttgtaa aacaaatgcc tgagggtgtg tacgtgcgct cggtaaaaca ggtgggtgtc
    20641 aatatctcgc ttgtaggata tgcacaatcc agtgcacgag tttccacatt gatgcgaaat
    20701 atcgaggctt ctccatggct tgaaaagccc cggttaatcg agatcaaggc gtcggtcgtt
    20761 gacaagcgac gcctgaacga attcattttg aatgcctccc tgaaacgaac ccctgttgat
    20821 gatggaggta agaaatgaaa gccccctcca tggccaaaat tgacatcaat gcgtttcttg
    20881 atgacttccg gaacctgaac cccaaggatg tgggcgcgtg gcccatcgca ccacgaattg
    20941 cggtattgat tggcattttc gttgcagcac tagtagcagg ctggtggttt gtatggaacg
    21001 atcagttgga gactcttgag acccggcaag cggatgagtt gaagctcaag gatgaatttg
    21061 tcgcgaagaa gaagcagtcg gttaatctgg ccttgtacac ccagcaatta agtgagatcg
    21121 accgttcatt cggtgccctc ctgaaacaac tgcccgacaa atccgaggtt gagtccttgc
    21181 tgatcgaaat taaccaatcc ggaatggggc gcgggctgca gtttgaacta ttcaagccag
    21241 gccaggaagt cagcaaggat ttctatgctg aattgcccat caatgttcga ataaccggga
    21301 gctaccatga ctttggcgcc tttgccggag atatcggcag gctgtcgagg atcgtaaccc
    21361 tgaacaacat ctccattact acagatccta agactaaaga tgggagtttg gtcatggacg
    21421 caattaccaa gacgttccgc taccttgacg aggaggaatt ggccgccaaa aagaaagcgg
    21481 ctcaagccgc caaaggtcaa aagaaatgaa gactatcgct gtcgcactgg ctagccttgc
    21541 cttggtggca tgctccggag gggataacga ggatctgcgt cagtggatga atgaggctgc
    21601 caaagatgca aaagggagca tccctcccct gccacaggtc aagccttatg aaccagtgcc
    21661 ttacgatgtg ggcaacctaa tggacccatt caagccagcc aggatcgggc cggatcagag
    21721 gaagggcgga ggcggtggat tacagccaga catggatcgg gcacgtgagc cattggagtc
    21781 tttcccactg gagtcgctga aatacgttgg tgtgatgagc aggaagaaga catcgtatgc
    21841 catcattttg gttgatggct ccttgtatca ggtgagggtt gggaattata tggggcagaa
    21901 tttcggggtg atcaccaaga ttttggaatc tgaattgact ttgaaggaac tgattcagga
    21961 ttctgcagga gactgggttg aaagagaaag ctcgctattg ctgcagggac aggagggaat
    22021 acagtgaaac aagttaaacg ttacctgctc agctttgtcg gcgggctgtt actggctatt
    22081 gccacagtca atgcacagga agccaagccg aattcaatcg aatccatgac cgtcgcgcaa
    22141 cagggcggtg tgctcaacgt gaagctggtt ttcaaggaac cgcttaacgc tttgccgccc
    22201 ggcttcagcg tggccaagcc ggcacgtatt gcccttgatt ttgcgaacac ggtcaatggt
    22261 ctcggcaaga acagtcaaac ctataacgaa ggtgatttga aaagcgccaa tattgttcag
    22321 gccgaagacc gcacacgctt ggtgttgaat ctcaatcagg cgatgactta cgagtcgaaa
    22381 atcgacggga acagcctgct gctgactttg gttccaagtg caaaagcgag tggagtccct
    22441 atagttgagc acttttccca gactcatccc gaacaggccg caaatagtat tcgtgatatt
    22501 gcatttcgtc gtgggaagga aggcgaggca cgcataacgg tcgatctaag tgacccgaat
    22561 gcgggaatcg atattcgtca gcaggggcaa aatctggtcg ttgatttcgt caaagtcagt
    22621 gttcccgatg cattgcgcaa gcgactggac gtaaccgact tcgcaacgcc tgttgtttca
    22681 ctgaacacga ttcaacaggg tgccaatgcg cgaatgacca ttaccccacg cggtttgtgg
    22741 gaacacaatg cataccagac agataatcag tttgtaattg aagtcaagcc ggtcatcgag
    22801 aatcccaata aactggtgca gggttcacga ggtggctatc agggggaaaa gctgtcgctg
    22861 aacttccaga acgtcgaggt gcggcgcctg ttgcaggtta tcggcgagtt taccggtatg
    22921 aatatggttg tcagtgattc ggttggtggg tcgatcacgt tgattctgaa agatgtgccg
    22981 tgggaccagg cgctggacat catcctccag caaaaaggcc tcgatatgcg gaagaacggc
    23041 aatgtcatcc tgattgcgcc ccgcgaagaa atagccacca aggaaaaact cgaatttgaa
    23101 tcgaaggcac aaatcggcga tctggagccg ttgcgaaccg aaagctacca gctgaattat
    23161 cagaaggcgg aagcaattca gaaattgctt accgatccta atcagcgcat gttgtccaaa
    23221 cgaggaagtg cagtcgttga tgcgcgtacc aatatccttt ttgttcagga cacacccagc
    23281 agactggagg acgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaatg
    23401 ccgagatatc cgcattagaa tctgatggaa agggcaaggt tatatccagt ccgcgtgtga
    23461 tgaccgccaa tcaggtggaa gcaataatcg aacagggcgt tgagatcccc taccagcagg
    23521 cgactagttc tggtgctacc agtgttactt tccgcaaggc gaatctgtct ttgaaggtca
    23581 agccccaaat cacacctgac ggcaagatca caatgacctt ggatatcaac aaggacactc
    23641 caaatacgcg cttgtccacc ggcgccggag tcgccatcga caccaagcat gtaaagaccg
    23701 aagtgctggt tgaaaatggt ggaaccgtgg taatcggagg tatctatact caggaagttc
    23761 gtgacaacac tcagcgcatt ccgttctttg gagatttacc ttacatcggc tggctgttca
    23821 agaatcgtga atggattgat gacaagaccg agttgctggt tttcattacg ccgaggattg
    23881 ttgcagaaag tctcgcggta cgctgaccct ttcaataagg acaagtgagc cggctgatgc
    23941 cggctttttt gtgtcgtttc ttagcgtacc cggtactgta gaatgcaatc gtggaaaaac
    24001 aatacggcac tgggaatgtc tatctggtcg gcctaatggg agccggtaag acgaccgttg
    24061 gccggtcgct ggccaagcgt ctggggctca ggttcatcga ttcagaccat gagattgagg
    24121 cgcgtacggg agtcagcatt ccgacagttt ttgaaatcga aggggaagaa ggtttccgca
    24181 aacgagaagc ccaggtgatc gctgacctgt cgcatttgag tgatcgtgtc gtcgcaacag
    24241 ggggaggtgt agtgttgcat ccggagaacc ggaccaactt gagggcaagt ggtttcgtcg
    24301 tttatctcaa cgttttgcca cacactttgt gggagcgcac ttcccatgat cgcaaccggc
    24361 cattgctgca agtcgctaac cccttgctca agctggagga actctactcg cagcgtgacc
    24421 cgctttatcg ggaagtggcg gatttagtcg tagattgtag ccggaccaat gcccacggtg
    24481 tcttgcagct attgaccaga gaagtcgggg aacgatggaa acactgacgg ttgcgctggg
    24541 ctcacgggaa tacccgattc ttgtcgggca gggcattctt gagaacggcg gacttcttct
    24601 cccctttctt cgttcgccaa aaattgccat tgtcacgaat acaactgtag cgccgcttta
    24661 cctggagcgt ttttcgactc ctctgcgtaa tgccggtatt gacatcgctg aaatcattct
    24721 gcccgatggc gagcagcaca agaactggca aacgttgaat acaattttcg atgtccttct
    24781 tgagcggcgt tgtgagcgat caagcacgtt gattggcctg ggcggtggcg tgatcggcga
    24841 tatggcaggc tttgcggcag cctgctacca gcgcggtatg ccgtttattc aagtgcctac
    24901 aaccttgctt tcgcaggtcg attcttccgt tggcggcaag acggctatca atcatccact
    24961 tggcaagaac atgattggtg ctttttatca accaaagctt gtactggctg atactgattg
    25021 tctgaatact ttgccagatc gcgagcttct cgccggactg gcagaggtaa tcaagtacgg
    25081 gttaatcagg gatttgccgt ttctggcctg gcttgaaact aacctcgaac gtttgttgga
    25141 acgagaaccc ggagcactgg aatatgccat ctgccgctcg tgcgcaaaca aggctgaagt
    25201 tgttgttgcc gatgagcatg aacacggcga gcgcgcattg ctcaacctgg gccacacttt
    25261 cggacacgcg attgaaacgg ggatggggta tggcaaatgg ttgcatggcg aggcggtagc
    25321 agcgggaacc atgatggcag cggaactttc acgtcagctc ggctggattg acctggatga
    25381 cctgaagcgt attgaaaatc tgttcgaacg cgctggattg cctgttttcg gcccagcaat
    25441 gagcgcagat cgatatctcg aactgatgca acacgacaag aaagttcagg acgggaaact
    25501 gcgacttgtg ctcttcaagc agatcggaca agccgtggtc agcgatatgg cgtcgccgac
    25561 agagatcggc aaagccatca ctgcacgctc caccgatgtt tgacctcgcc ccttatgcnn
    25621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gcaactagag atcaagcagt
    25741 tcctgcgcag gcacctctat caacattttc gtgttttgcg catgaccggc aaagcacaac
    25801 gaatcatccg tgatcttttc tcagccttca caagcgacac acgcttacta ccgccgcagt
    25861 atcaggcagc cggagaccca ttccagattc gcagggtagc cgattacatt gccggaatga
    25921 cggaccggta tgcaataaag gaacaccggc gtttgtttgc tgtcggagac acgtgaaaga
    25981 ccagttggac cagtactgct gacgcgcagc tactcctctg gccaatcctt gatataagcc
    26041 ttgagcagct tgttttcaaa gttgcgttcc tcaagcacgg cttttgcgac atcatgaaat
    26101 gaaatgactg cggtcatggt gtcgccatcc attaccggca cgtaacggga atgggtatcg
    26161 atcatcaatc tgcgcaattc gtcgacgtcc atcgaagggt cggtcgtcgc cgggtcgatg
    26221 tgcatggctt cgctgaccag tatctcggca attggcggtg tcgggccgct acggcgttct
    26281 gtctggcgct tggccaaaac ctgaagcacc tcacggaaag tcagcattcc caccagtttc
    26341 ccgtcatcca tcacgacaac cgagccaatg tcgttttcgg ccatcatgat gacagcttcg
    26401 gagaggagcg attccggatg agctgcaaat atcgcagaat tcttgacctt cagtacttcc
    26461 ctgacctcca tgctaccccc tgaagaaaag catttgatcc ctgtatccac cctcttgggc
    26521 aaaactcgac cgatctcaga cgcttgacag cacccgcgca aacgggtgct gtcgcagcct
    26581 aatatgcaac cgacgatata gtcaatcagc ccttcaagac gaccagaacc tcgtccagca
    26641 tcttcttggc gtcaccgaaa agcatacggt tgttttcctt gtagaacaag ggattgtcga
    26701 cgccggcata acccgacgcc atgctgcgct tcatcacgat ggatgtcttt gccttccagg
    26761 cctcaagtac cggcatgccg gcaatcggac tgttcgggtc atcctgcgct cccggattca
    26821 caatgtcgtt ggcaccgatc accatcgcca cgtccgtttt cggaaagtcg tcgttgagtt
    26881 cgtccatctc catcacgatg tcgtacggaa ccttggcctc ggccagcaac acattcatgt
    26941 g
//
LOCUS       DAORPM010000002        26785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_274_length_26785_cov_9.311036, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000002 DAORPM010000000
VERSION     DAORPM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 26785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26785
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_274_length_26785_cov_9.311036"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLD53_00135"
     CDS             complement(<1..496)
                     /locus_tag="PLD53_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HRC59339.1"
                     /translation="MLSRAVVALLWFLHFLPLSILAMIGNGLGSLLFRFAKKRRHIVH
                     TNLALCFPELDDAARKTLALKHFRVLGRSMLERSLFWWARRERLQRLIRVEGEENVHA
                     LRDAGKPVLLLAPHFVSLDAGGAAVAMRFDCVSIYAEQSNPLFDELLLGGRNRFGKQQ
                     LLSRR"
     gene            663..1184
                     /locus_tag="PLD53_00140"
     CDS             663..1184
                     /locus_tag="PLD53_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC59340.1"
                     /translation="MSNSTGITAEITLVRPADVSAIVLLARVIWQHAYAGMITQMQID
                     YMLAQRYNAPHLLTELSQPGLWWDQAFVTGERVGFSSCYLTDTPGEIKLDKVYVHPDW
                     QRAGVGGALIERVVGRGRAACCDTLVLAVNKQNVRAIAAYERKGFSVRESVCVNIGGG
                     FVMDDFIMSRSIA"
     gene            1191..2021
                     /gene="dapF"
                     /locus_tag="PLD53_00145"
     CDS             1191..2021
                     /gene="dapF"
                     /locus_tag="PLD53_00145"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRC59341.1"
                     /translation="MKLKFSKMHGLGNDFVVLDGVRQSIDLSPEQLRLLASRNFGVGC
                     DQILLVEKPSQAGVDFGYRIFNADGGEVEQCGNGARCFVRFVHDQGLTEKHEIRVETT
                     GGILGLRLEADGAVTVDMGVPVFAPEKIPFLSQTDELVQSLTIGNEAIEITAVSMGNP
                     HAVHVVTDIDTAPVASWGPLIESHPHFPQRVNAGFMQIVGRRAIRLRVFERGAGETLA
                     CGSGACAAVVAGIARGLLDSPVRVTTHGGELTIAWGGEGTSVLMTGPAVTVYTGEIEI
                     "
     gene            2018..2671
                     /locus_tag="PLD53_00150"
     CDS             2018..2671
                     /locus_tag="PLD53_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HRC59342.1"
                     /translation="MKSEEIAKYLQDNPQFFEEHADLVARIVIPHPHGGRTISITERQ
                     MLSLRDKNKLLEAKMDELLQFGEENDTISEKMHRLAVAMIAASSFQSVLHTLNFHLRD
                     DFSIPHVALRLWDRSDNTDELPEFANVSEELQVFAETLSQPYCGSTSGFETSSWFGDA
                     AEHIRSQALIAMRSGGGTVGMIALGSEEPQRFYVGMGTLYLERLGEMASAALVRVLR"
     gene            2690..>2886
                     /locus_tag="PLD53_00155"
     CDS             2690..>2886
                     /locus_tag="PLD53_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRC59343.1"
                     /translation="MLTDGLVSAYLDELAQQRRQSPHTVSNYRRDLGVLGRLVGELPG
                     NHRFDTIQTHQIRRFIAQLHAR"
     assembly_gap    2887..2986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2987..3385
                     /locus_tag="PLD53_00160"
     CDS             <2987..3385
                     /locus_tag="PLD53_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRC59344.1"
                     /translation="VRVLGKRNKLRLVPVGSKAREAVGLWARRRGEVASAEQIALFVG
                     QRGGRISPRLIEERLKRRALAKGLLTGVHPHMLRHSFASHVLQSSGDLRAVQEMLGHA
                     SIASTQVYTHLDFQHLARVYDSAHPRAKRK"
     gene            complement(3393..4031)
                     /gene="msrA"
                     /locus_tag="PLD53_00165"
     CDS             complement(3393..4031)
                     /gene="msrA"
                     /locus_tag="PLD53_00165"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRC59345.1"
                     /translation="MMKRIAMGLTMALGVFAVAASFLTGTSATAADTPAVKPGNTATA
                     IFASGCFWCTESDFEKLPGVIGAESGYTAGKTPNPSYEQVSAGGTGHTEAVRVTYDPA
                     KVSYTQLVVHFWHNVDPTAKNRQFCDSGSQYRSGIYWQNAAEREIAESSRDALLASGK
                     FKEIHTEIIAATTFYPAEEYHQGYYKKNPIRYNYYRAGCGRDARLEQLWGKK"
     gene            complement(4028..4540)
                     /gene="msrB"
                     /locus_tag="PLD53_00170"
     CDS             complement(4028..4540)
                     /gene="msrB"
                     /locus_tag="PLD53_00170"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRC59346.1"
                     /translation="MDRRQYLKLLGGLTTCFLPIPAMQMVIAKEGGTMQLVKSKSEWS
                     ALLPPAAYRVLFEEDTERAGTSALNDEKRDGTFICAACFLPLFDSKTKYESGTGWPSF
                     WQPLPEAVATKTDFKMILPRTEYHCVRCGGHQGHVFNDGPKPTGKRYCNNGVALKFVP
                     REDKLPELRT"
     gene            complement(4585..5382)
                     /locus_tag="PLD53_00175"
     CDS             complement(4585..5382)
                     /locus_tag="PLD53_00175"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HRC59347.1"
                     /translation="MSHLTIASKVAVALSLALPMTASLAASCSAKSPAHTVALVELYT
                     SEGCSSCPPADRWLGELPRRFTAEQVLALSLHVDYWDYIGWKDPFAQAQFSDRQRWLS
                     QLASSATIYTPEVFAGMKELRAWSNRQSFENRIQSINRQPARAQITVQMQSSGGDSVD
                     LEARFALDDEARTGRTGQGVVVLYEKHLSTEVRAGENRGSRLNHDNVVRFWSAPVTLD
                     AQTGRAEWKQTVTLPSGWKRENLGMAALVQDAKAGEVLQAVSLPGCA"
     gene            6028..7239
                     /locus_tag="PLD53_00180"
     CDS             6028..7239
                     /locus_tag="PLD53_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRC59348.1"
                     /translation="MVARPAVIPRAKEAPEVALPPAEDKLDSSAGSPQSGTVPPPEAA
                     SDDLSSLDFVKSDFSEFDFSEVSPDFQIEQDVDPVDAEAEEAAILYANGQDEAARSVL
                     ENAVRIHHFGPGERLWMMLFDLYKLTGQKAAFDALGIDYARSFEKSPPAWRDKSTGQA
                     KVSEAVAGSLLFKGDLTGENDGAFEAVRQSLAKNVRLRLDMSRVSQIDAVGCGLLLTL
                     LQQARKSRREVELLGRDALAEMLEQRIVAGSAEDSECWLLLLELCQLQGRNEAFEEVA
                     INYAVTFEVSPPSWESSRVAAPEPAKQMAADVPGDDGVQDAYVLRGDVKSSRFGDLTA
                     HADLHDPVLIDCAPLVRLDFISAGALLNVLSTIRQTGKQIVFRHPNHLIAELFRVIGL
                     RTVAAIIFAKH"
     gene            7249..7785
                     /gene="hslV"
                     /locus_tag="PLD53_00185"
     CDS             7249..7785
                     /gene="hslV"
                     /locus_tag="PLD53_00185"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRC59349.1"
                     /translation="MEQYHGTTILSVRRGKRVAMGGDGQVTLGNIVIKAGARKVRRIH
                     KGDILAGFAGGTADAFTLFERFEAKLDKHQGNLMRSAVELAKDWRSDRALRRLEAMLA
                     VADHENSLVITGNGDVLEPEQGIVAIGSGGAFAQSAARALIENTELDPAEIIRKSLEI
                     AGDLCIYTNHKLTIETLD"
     gene            7874..9205
                     /gene="hslU"
                     /locus_tag="PLD53_00190"
     CDS             7874..9205
                     /gene="hslU"
                     /locus_tag="PLD53_00190"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRC59350.1"
                     /translation="MSQMTPQEIVRELDKHIVGQAKAKKAVAIALRNRWRRAQVNEPL
                     RQEITPKNILMIGPTGVGKTEIARRLARLANAPFIKIEATKFTEVGYVGRDVETIIRD
                     LAEIAIKNGRDMAMSGVRERAADAAEERILDALLPPARGNFFNDTQAAEENATRQKFR
                     KKLREGTLDDKEIEIEVAGPAMQAEIFAPPGMEELTSQIQGMFQNMGGARKKSRKLKI
                     SEARKLLIDDEAGKLVNDEEVKLNAVRDVEQNGIVFLDEIDKITSRSDAHGADVSRQG
                     VQRDLLPLVEGTTVSTKYGMIRTDHVLFIASGAFHLAKPSDLIPELQGRFPIRVELDS
                     LLVADFEQILTQTDACLTMQYQALLATEGVTLEFQPDGIRRLAEVAWSVNERTENIGA
                     RRLYTVMERLLEEVSFSAGSAGEETISINAEYVDGRLNELAKNEDLSRYVL"
     gene            complement(9202..10167)
                     /gene="hemF"
                     /locus_tag="PLD53_00195"
     CDS             complement(9202..10167)
                     /gene="hemF"
                     /locus_tag="PLD53_00195"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRC59351.1"
                     /translation="MDSSALKTYFVGLQSRIVAAAEALEGENGGRFLADTWQKEAGQP
                     LGGHGLTCIIEGGRIFERGGVSFSHVTGSALPASATAKRPELAGRAFEAMGVSLVLHP
                     ENPYCPTAHMNVRCLLAHPGGERNADSPGADAGRTNSNTTTLWWFGGGMDLTPYYPFE
                     DDIRHFHATCKSALTPFGDDVHARYKKWCDEYFFLKHRNEPRGVGGVFFDDLNEGGDA
                     RCFALTRAVGDAFSAAYLPIIEKRQETPYGERERDFQAYRRGRYVEFNLVWDRGTLFG
                     LQSGGRTESILMSLPPVVKWRYDWAPEAGTPEALLYEILPPRDWV"
     gene            complement(10167..>11293)
                     /gene="purD"
                     /locus_tag="PLD53_00200"
     CDS             complement(10167..>11293)
                     /gene="purD"
                     /locus_tag="PLD53_00200"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRC59352.1"
                     /translation="ALADFAEKEGIHLTVVGPEAPLAAGVVNIFRARGLKIFGPTREA
                     AQLESSKDFAKRFMARHNIPTAAFETFSDSVAAHAFVDKHGAPIVIKADGLAAGKGVV
                     VAMTLGEAHSAIDSMLPGNKSNMTGDKSGSRIVIEEFLDGEEASFIVMVDGKNVLPLA
                     SSQDHKRIGDGDTGPNTGGMGAYSPAPVVTPTIHAKAMREIILPTVRGMAADGTPYTG
                     FLYAGLMIGKDGSVKTVEFNCRMGDPEAQPIMMRLKSNFLTLLEHAVVGKLDQIDAEW
                     DRRVALGVVLAAANYPGTPRQGDVISGLPDESDDVHVFHAGTQEKDGRILTSGGRVLC
                     VTALGDNVKQAQKQAYDAIVGIHFDGMQYRRDIGHRAIPR"
     assembly_gap    11294..11393
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11412..12989)
                     /gene="purH"
                     /locus_tag="PLD53_00205"
     CDS             complement(11412..12989)
                     /gene="purH"
                     /locus_tag="PLD53_00205"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRC59353.1"
                     /translation="MKIRQALISLSDKRGALEFAQGLAAQGVKLLSTGGTAKQLRGAG
                     LDVCEIGDYTGFPEMLDGRVKTLHPKVHGGILARRDLPEHLATIARHDIPTIDLVCVN
                     LYPFRETIAKPDVTLEDAIENIDIGGPAMVRSAAKNYTGVAVVTDPEDYTALLAEMLA
                     NDGALSLATRFGLAKKAFTHTARYDATIANWLTALDETNKPKQFPDKLQLAFDCVETL
                     RYGENPHQQAAFYRDPIPTPGSIAAYKQLQGKELSYNNIADADAAWECVKTFTGGANS
                     AACVIIKHANPCGVAIDSTLLSAYEKAFKTDSSSAFGGIIAFNGTVDTDVIKAMNERK
                     HFVEVLIAPAYTPAAKEMLASKQNLRVLELPLSNNCPPFEFKRVGGGLLVQTPDTFNV
                     QASDLKVVTHAAPTSAQIEDLLFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDST
                     RIAASKAQNAGLVLAGSCVASDAFFPFRDGLDVLAEAGAKSVIQPGGSMRDDEVIAAA
                     NEHGLAMVFTGARHFRH"
     gene            complement(13081..13317)
                     /locus_tag="PLD53_00210"
     CDS             complement(13081..13317)
                     /locus_tag="PLD53_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC59354.1"
                     /translation="MKEHNDISSCIYSALETYFKNLDGEMPAPIYDMVLKSVERPMFE
                     VIIKQAGGNQTQAAAMLGINRNTLRKKLVDYKLV"
     gene            complement(13314..14348)
                     /gene="dusB"
                     /locus_tag="PLD53_00215"
     CDS             complement(13314..14348)
                     /gene="dusB"
                     /locus_tag="PLD53_00215"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRC59355.1"
                     /translation="MEFVGFTLRNNLFVAPMAGVTDRPFRQLCKKLGAGLAVSEMVTS
                     NSLLYGSAKTKRRANHDGEVEPISVQIAGAAPAMMADAARHNVDRGAQIIDINMGCPA
                     KKICNVMAGSALLKDESLVGRILESVVNAVPETPVTLKIRTGWDKSSRNALSILKIAE
                     QSGIRALAMHGRTRACGYSGQAEYETIAAVKREACIPIIANGDINSPEKAKYVLDATG
                     ADALMIGRAAQGRPWLFREIEHFLKTGTHLMPPRVNEIHTILLAHLEDLYEFYGIETG
                     VRVARKHISWYTKGLVGSAAFRHSMNQLPDIRQQQQAVNDFFFGLAEHNEHLNYIQTE
                     HKNDWEELAA"
     gene            complement(14456..15637)
                     /locus_tag="PLD53_00220"
     CDS             complement(14456..15637)
                     /locus_tag="PLD53_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRC59356.1"
                     /translation="MTEHPAGATSPALSPPYDADVLIIGAGPVGMALALALAGSRQRV
                     ILIDSRQRGAWIDDPRALALSHGSRQLLERLNAWNAMAGTPIREIHVSQRGGFGRTML
                     SAKDYGTPALGYVMRYRDLAAALAARIKPEQLIDQCSVDTIETGREETTLTFTREGTI
                     HRITAKLIVHAEGTPERGPEITVRDYDQHAIVAEVRPATGHNNRAWERFTPDGPLALL
                     PLGRDYSVVFTVRPAKAARLLKLNDENFLAALQEQFGKRLEFVSTGPRAGFPLALRCR
                     TQLAQRRQVWIGNTAQSLHPVSGQGFNLGLRDAWELAESLFDQSDAGNPTALATYAKG
                     RQFDRCGSIAFTDGIVRLFSNDLAPISFARGIGLLALDLYPPLRHFVAKRMIWGARAW
                     P"
     gene            complement(15630..16940)
                     /locus_tag="PLD53_00225"
     CDS             complement(15630..16940)
                     /locus_tag="PLD53_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRC59357.1"
                     /translation="MKHEPYSTRRSALLEQIGDGVAIIPTAPERVRNRDSHHPYRFDS
                     YFWYLSGFPEPEAIIVLLGGNDSQPVSRSIIFCRERNEEREVWDGFRYGPEAVCDAFG
                     FDEAYAFAEFEHRLPDLIASRSRLWYAFGHEAAVDTDVISALNTVRAQSRAGKRAPGE
                     IRDLREPLDRMRLIKDAHETDIMRRAAAIAAAGHARAMRTCRPGMAEYELEAELGYEF
                     RKRGADGHAYSPIVAGGHNACILHYIENNRLLSEHALVLIDAGCELEGYASDITRTFP
                     VSGRFSGVTRDAYEIVLAAQTAAIAAIKPGAPFIAYHDAALRVLVQGMIDLQLLAGSV
                     DGIIESETYKPFYMHRTGHWLGLDVHDAGEYKSGDDWVVLQAGMALTVEPGLYIRPGA
                     TVPEHLHGIGIRIEDDVFVTTDGCEVYTSAPKSIAEIEEVMRRD"
     gene            complement(16937..17641)
                     /locus_tag="PLD53_00230"
     CDS             complement(16937..17641)
                     /locus_tag="PLD53_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRC59358.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLHAGGKPLIVWHLERLAAS
                     GFRDIVINHSHLGEQIEATLGNGNRFGLNILYSPEPAGALETAGGIAQALPFLGSQPF
                     LVVNGDVWCDWDFSQAHKRMHALTGHPAHLVLVDNPPQHAGGDFSLDGETVRYAGEST
                     GPTLTYAGTGIFSPDFFAAVPAGAVMKMRPLLDAGIAKGLVTGERHPGIWVDVGTPER
                     LAELDRELKAKEFALS"
     gene            complement(17706..18758)
                     /locus_tag="PLD53_00235"
     CDS             complement(17706..18758)
                     /locus_tag="PLD53_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRC59359.1"
                     /translation="MPRSALLRSWLDKLFASQYPDKDFLVEPASADASFRRYFRVSLP
                     DGNTRIVMDAPPEREDCRPFLKVAALFSAAGVHVPTVYAEDLDQGFLLLSDLGSATYL
                     DVLNLDTAPRLYRDANAALVTIQTASRPGVLPDYDRALLERELELFPDWYVARHLGAK
                     LDVDQQTTLRTVFDRILANNLAQPRVYVHRDYHSRNLMVCGMAGSNGTSARASSIHEN
                     PGIIDFQDAVYGPITYDLVSLYRDAYIHWEEAQELDFVIRYWEMARKAGLPVHADFHD
                     FYRDYEWMGAQRQLKVLGIFARLCHRDGKESYLKDMPRVLSYLRQTCERYLELRPLAR
                     LLDQLENRQVVAGYTF"
     gene            18874..22497
                     /gene="pagP"
                     /locus_tag="PLD53_00240"
     CDS             18874..22497
                     /gene="pagP"
                     /locus_tag="PLD53_00240"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HRC59360.1"
                     /translation="MNFPARRKSRALLICYGFACLLFGGHTVAQEQALAPLRVNPALL
                     GLPPIKPAEAPAIAPVAPPATADKGPEVKPVQAVVVEPKAVDRAPEASESARARKDTS
                     LETPSHQPRGEGQQAAPVAPVPQMEKQPRSEPQAPTVAQVQSAKPVARQQAQPASRKP
                     AETDKSWFQRVWSPVANAYNNGALEFYLPLKTYHSRSTYSQEQIDSYQENPLGFGIGR
                     GLYNEKGNWEGVFAMAFQDSHFKPSYTGGYQWKAIWRPAEDVRLGLGYLAGLMSRSDI
                     LSYVPFPIVFPVASVAYKNFSLEGTYIPPGGDNAGNVLFIWAKWEMGKTGEAIGTPAQ
                     PAPPTPTEMANASFGPSTPVTRQRVPYGPVLEPGSTPRLVSSSETVPPVVRATGRRDE
                     EEVPDPLPSLVLQSSRSMVTPPKDSAVSRPVFLSAYRMGGDIDREFNAEGEAELRKIG
                     TVVNADRLTYWPIDDEIEAEGNARLEQGEDLITGPKMRLKLEDQVGFFEQPSYTIKRQ
                     PSSGGKAKAETESTALSAEQQDEDSWLTSGFASPQVLDIKPGQTSFDYSKQSPRRATV
                     ARGEADRIDFEGENQVRMTNATYTTCEPGNDDWYAKAGSLKLDYDREVAEGTDGTVYF
                     LGTPILYSPWLSFSLNNQRKSGFLAPTFGTSSNRGIELAMPYYWNIAPNMDATIAPRV
                     MTKRGVQLNNEFRYLNTAFGGTYRGELHAEVLPNDRERNGENRYGYSVQHNQAMANGF
                     SGVINYGRVSDDDYFTDLSSQISHTSQVNILQQGVLSYGGGGWWNATANFQQYQTLQP
                     DPENPTLYPYQLLPQFTVTARRPDLYMTDTSFFGQYTNFTIKERIQNVDGVPTLYPDG
                     QRTVLYPQVALPYVTPGWYVTPKIGVNVRHYELSGQAAGTPGSINTTLPIFSIDSGMT
                     FERPSNWFGRDYTQTLEPRLFYVNIPYKNQEQIPIFDSGLADFNFAQIFSENQFSGWD
                     RINNANQLTAAMTTRLIEPSSGNEIMRAMFGQRFYFTRNKVELNASTSTPTEDQSWQK
                     SDVLAAFSGQILPKVYADAAWQYNLNDRQVNRFSMGGHYRPEPGKVFNAAYRYNRDPN
                     APIDQVDFSGQWPLTGRWTAVGRFNFSFKDDATNTSTATQNGRLIESIAGLEYNGGCW
                     VVRGVAQQIALTQDKTSTGFFIQLELNDFSRIGSNPLNLLKRNIQGYSLINQPAADPV
                     FGE"
     gene            22510..23820
                     /locus_tag="PLD53_00245"
     CDS             22510..23820
                     /locus_tag="PLD53_00245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC59361.1"
                     /translation="MTQLLRTLVLYFCFVGLAASQSPSRQVREPLPADRIVAVVNDEV
                     ITLHELRTRLDLALNQLKKQGTPLPARDVLERQMLERLVMDKVQLQFARETGLRVDDA
                     QLEQALQRIAAGNKMSLSQFREALQKDGIPFARFREDIREEMTISRVREREVENKITI
                     SEGEIDNYLSANSAKGGAGEEYEVAHILLRVPESASPEQIQKLKAKADEVMGRLKKGE
                     DFAQLAAAYSDAPDGLQGGNLGMRPLDRLPGSFGELVIKLQVGEVAPILRSPNGFHIV
                     KLVSKRGGSDMPAVQQTHARHILIKVNEVVSESDARHTLAGLSERIKNGASFAELARL
                     YSQDGSASKGGDLGWIYPGDTVPEFERAMNKLKPGEVSQAVHSPFGFHLIEVLERRVQ
                     DVSAERQRVIARQVLRERKMDEAYQDWLRQARDRAYVEIRIEER"
     gene            23824..24810
                     /gene="pdxA"
                     /locus_tag="PLD53_00250"
     CDS             23824..24810
                     /gene="pdxA"
                     /locus_tag="PLD53_00250"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRC59362.1"
                     /translation="MLARPLVAVTSGEPAGVGPEICLRLMDGVTGALNADLVVLGDRH
                     LMAARAEAISFTGELRDWSPGLRPLPGHLDILHVPLSVPSRPGHLDPENSSYVLQLLQ
                     RAIEGCRTGEFSAMVTAPVHKGVINDAGIPFTGHTEYLAEQTQTPRVVMMLTGGGLKV
                     ALATTHLPLKEVPSALTQNSIEQTLRILHADLVRKYAIEDPRILVAGLNPHAGEGGYL
                     GREEIEIIAPVLDRLRAEGMRLIGPLPADTMFTPPMLALGDCVLAMYHDQGLTALKYA
                     SFGAGINVTLGLPIIRTSVDHGTALELAGSGRADPGSLFVAVEQAIEMAQRQ"
     gene            24970..25749
                     /gene="rsmA"
                     /locus_tag="PLD53_00255"
     CDS             24970..25749
                     /gene="rsmA"
                     /locus_tag="PLD53_00255"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRC59363.1"
                     /translation="MNQHVPRKRFGQNFLIDRQVIAAIVDAVAPERDDCVVEIGPGLG
                     ALTDPLLKLLDHLQVVEIDRDIIARLKTRYPPGKLTLHEGDALAFDFASLCKGSSAGL
                     RIVGNLPYNISTPLLFHLAGFADQVVDMHFMLQKEVVERMVAEPGTPDYGRLTVMLQY
                     RFVMDWLLDVPPESFDPAPKVDSALVRLIPRLPADLSAKDESRFAAIVSAAFAQRRKM
                     LRNNFKGIFDDTDYGRLGISSTARAEELSVEDYIRIANSMR"
     gene            complement(25864..26061)
                     /locus_tag="PLD53_00260"
     CDS             complement(25864..26061)
                     /locus_tag="PLD53_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRC59364.1"
                     /translation="MKANVGGLDRILRIAVGIVLIALAAMGVVGVWGWIGVVPLLTGL
                     IGWCPAYPLLGISTCPMKKQN"
     gene            complement(26263..>26785)
                     /locus_tag="PLD53_00265"
     CDS             complement(26263..>26785)
                     /locus_tag="PLD53_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC59365.1"
                     /translation="GFPLLLSGTIKVVKSMPAGREMLLYRIEPGGSCVITSSCLLGHM
                     PYSARGIAETALQIAILPKTLFERLIAGDATFRDFVFHLLADRIADLMQLVEEVAFHR
                     LDQRLAKLLLGKSEPIQATHQGLADELGSVREIVSRLLKGFAAQGFVSIGRERISLLD
                     RDGLKQLAGIDRK"
BASE COUNT         5514 a         7313 c         8002 g         5756 t
ORIGIN      
        1 cgcgacgcga tagtagttgc tgcttgccga agcggttgcg accgccgagc agcaactcgt
       61 cgaataacgg attgctctgt tcagcataga tgctgacgca atcgaagcgc atggccaccg
      121 cggcgccgcc ggcatcgagg ctgacgaagt gcggcgccag cagcagcacc ggcttgccgg
      181 cgtcgcgcag ggcatgcacg ttttcttcgc cttcgacgcg aatcagtcgc tgcaggcgtt
      241 cccggcgcgc ccaccagaac aggctgcgtt ccagcatgct gcggccgagt acccgaaaat
      301 gcttgagtgc cagcgttttt cgggccgcgt cgtcaagttc cggaaaacag agtgcgagat
      361 tggtgtgcac gatatgtcgc cgcttcttgg caaaccggaa gagcaggctg cccagcccgt
      421 tgccgatcat tgccagtatc gaaagcggca aaaagtgcaa gaaccagagg agggcgacaa
      481 cggcgcgtga gagcattttt ggctatcgaa ctgggtgggg cttttgaaga ggcgaaagta
      541 tcaccggcat cgcggccgag gtcaaacttc gttctggcga ggaatagctg tcgcccaatt
      601 gcacgcaaac gttgagcaac ggataatcgg atagtccgca atgagccagg acagtatcgc
      661 gtatgagtaa cagcaccgga atcaccgccg aaatcaccct ggttcgaccc gccgacgtaa
      721 gtgctatcgt ccttttggct cgtgtcatct ggcagcatgc ctacgctggc atgattacgc
      781 agatgcagat cgactacatg ctggcgcagc gctacaacgc gccgcacctg ctcactgaat
      841 tgtcgcaacc cgggttgtgg tgggatcagg cctttgttac cggcgagcgg gtcgggtttt
      901 cctcctgcta cctgaccgat acgccgggcg agatcaagct ggacaaggtt tatgtacatc
      961 cggattggca acgcgccggt gttggcggcg cgctgataga gcgggttgtc gggcgtggtc
     1021 gtgcggcctg ctgcgatacg ctggttctgg cggtgaataa gcagaatgtc cgtgccattg
     1081 ccgcttatga gaggaagggc ttctcggtac gtgagtcggt gtgcgtcaat attggcggtg
     1141 gatttgtcat ggacgacttt ataatgagcc gttccattgc gtaggccacc atgaaactca
     1201 agttctcgaa aatgcacggg ttgggcaacg acttcgttgt gctcgacggt gtgcgccagt
     1261 cgatcgattt gtcaccggaa cagttgcgcc tgctcgccag ccgcaacttc ggtgtcggct
     1321 gcgaccagat actgctggtc gagaagccgt cgcaagccgg tgtggatttt ggctaccgca
     1381 ttttcaatgc cgatggcggt gaggttgagc agtgtggcaa tggtgcacgc tgcttcgtgc
     1441 gttttgtgca cgatcagggt ttgaccgaaa agcacgagat tcgtgttgaa acaacgggcg
     1501 gtatccttgg cctccgtttg gaggccgatg gggctgtcac ggtggacatg ggtgtgcccg
     1561 tgtttgcacc ggagaagatc ccgttcctca gccagacgga cgaactcgtt caatcgctca
     1621 cgattggcaa cgaggcaatc gagatcacgg cggtttcaat gggcaatcct cacgctgtgc
     1681 acgtggttac cgatatcgat acggcacccg ttgcttcttg ggggccgttg atcgagtcac
     1741 acccccactt tccgcagcgg gtcaatgccg gcttcatgca gattgtcgga cggcgggcga
     1801 ttcgactgcg tgttttcgaa cgtggggccg gcgaaacgct ggcctgtggt tccggcgctt
     1861 gcgccgccgt ggtcgcggga attgcgcgcg gcctgctcga ctcgccagtg cgtgttacaa
     1921 cgcatggcgg agagcttact attgcctggg gcggcgaggg cacatccgtg ctgatgaccg
     1981 gcccggcggt tactgtctat accggagaaa tcgagatatg aaatcagaag agatcgccaa
     2041 atacctgcag gataatccgc agttcttcga agagcatgcg gatctggttg cgcgcattgt
     2101 tattccgcat ccgcatggcg ggcgaaccat ctcgattacc gagcgccaga tgttgagttt
     2161 gcgcgacaag aacaagctgc ttgaagccaa gatggacgag ttacttcagt tcggtgagga
     2221 aaacgacacg atcagcgaaa agatgcatcg cctcgcggtc gcaatgattg ctgcttccag
     2281 ctttcagtcg gtgctgcaca cgctgaattt tcatttgcgc gacgacttct ccattccgca
     2341 cgtggccctg cgcctgtggg atcgatcgga caacacggat gaactgcctg aatttgccaa
     2401 cgtcagcgaa gaattgcagg tgtttgccga gacactcagt caaccctatt gcggttcgac
     2461 ttccggcttt gaaacctcat cctggttcgg cgatgccgcg gagcacatac gctcgcaggc
     2521 tctgatcgcc atgcgtagcg gaggcggtac cgttggaatg atcgcgctcg gtagcgaaga
     2581 gccgcagcgc ttctatgtcg gaatgggcac cttgtatctt gaacgccttg gcgagatggc
     2641 ctcagcggcc ctcgtgcggg tattgcgctg atctctacag ggcaattgca tgctcaccga
     2701 cgggctggtc agtgcttacc tcgacgaact cgctcagcaa cggcgacagt ccccgcatac
     2761 ggtcagcaac taccggcgtg atctgggtgt tctcggcagg cttgtcggcg agttgcccgg
     2821 aaatcaccga ttcgacacga tccagacgca tcaaattcgc cgtttcatcg cccagttgca
     2881 cgcccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgc gtgttctcgg
     3001 caagcgcaac aagctgcggc tggttccggt cggcagcaag gcccgcgaag ccgttgggct
     3061 ttgggcgcgg cgacgaggtg aagtggcttc cgccgagcaa atagcgctat tcgtggggca
     3121 gcgcggtggc cgaatcagcc ccaggttgat cgaggagcgg ctcaagcggc gtgcgctggc
     3181 aaaaggtttg ctgactggcg tgcatccgca catgctgcgc cattcatttg cctcgcatgt
     3241 tttgcagtcg tccggcgacc tgcgtgcggt gcaggaaatg ctcggccatg ccagcatcgc
     3301 gtcgacgcag gtctatactc accttgactt tcagcatctg gccagggtct atgattcggc
     3361 gcacccccgc gccaaacgga aataggggcg gcttacttct ttccccatag ttgttcgagt
     3421 cgtgcatccc tgccgcagcc ggcacggtaa tagttgtacc gaatcggatt cttcttgtag
     3481 taaccctgat ggtattcctc ggccgggtag aacgttgtgg cggcgatgat ctcggtatgg
     3541 atttccttga atttcccgct agccagcaac gcatcgcggc tgctttcggc gatttcgcgt
     3601 tccgccgcgt tctgccagta gatgccgctg cgatactggc tgccgctgtc acagaactgc
     3661 ctgttcttcg ccgtcgggtc aacgttgtgc cagaaatgga ccaccagttg tgtatagctg
     3721 accttggccg gatcgtaggt caccctgact gcttccgtgt gcccggtgcc gccagcgctg
     3781 acctgttcgt agcttggatt gggcgttttg cccgcggtgt agcccgactc ggcaccaatg
     3841 acccctggca gtttttcgaa atccgactcg gtgcaccaga agcaaccgct ggcaaaaatg
     3901 gccgttgcag tgtttccagg cttgactgcc ggggtgtcgg cagccgtagc gctggttccg
     3961 gtcaggaaac tggcagctac ggcaaaaacg cccagcgcca tcgtcaggcc catggcaatt
     4021 cgcttcatca tgtccgtagc tccggaagct tgtcttcgcg tggtacgaac ttcaacgcaa
     4081 cgccgttgtt gcagtagcgt ttaccggttg gtttcggacc gtcattgaaa acatgcccct
     4141 gatgaccacc acaacgaacg cagtggtact cggtacgtgg cagaatcatc ttgaagtcgg
     4201 tcttggtggc gacggcttca ggcagaggtt gccagaagct gggccagccg gtcccacttt
     4261 cgtatttcgt cttgctgtcg aacaagggca ggaaacatgc ggcgcagatg aaggtcccat
     4321 cgcgtttctc gtcgttgaga gcactcgtgc cagctcgctc ggtgtcttcc tcgaacaaga
     4381 cgcgataggc ggcaggtggc agcagggctg accactcgga ctttgatttt acgagctgca
     4441 tcgtgccgcc ctccttggcg ataaccattt gcatggctgg tatgggtaga aaacaggtgg
     4501 ttaaaccacc gagtaacttg agatattggc gacgatccat ggcttcctcc aaaaactgtc
     4561 gtgagtctag cggcgactat tcacctaagc acatccgggc agtgaaaccg cttgcaatac
     4621 ttcacccgcc tttgcatcct gcaccagagc cgccatacca aggttctcgc gtttccagcc
     4681 agagggtagt gtgacggttt gcttccactc cgcccggcct gtttgcgcgt ccagtgttac
     4741 cggtgcagac cagaagcgca ctacattgtc gtgattcaac cgggaacctc tgttctcacc
     4801 agccctgact tcggtggata ggtgtttctc atatagaaca accactccct gcccagttcg
     4861 tcccgttcgt gcttcgtcat ccagcgcgaa ccgtgcttca aggtcgactg aatcgccgcc
     4921 agacgattgc atttgcacag tgatttgggc acgagccggt tgacggttaa tgctctgaat
     4981 acggttttca aagctttgtc ggttgctcca agcgcgcagt tccttcatcc cggcaaagac
     5041 ctcaggagtg tagatggttg cgcttgaggc caactggctc agccagcgtt gccgatcaga
     5101 aaactgggcc tgagcgaaag ggtctttcca gccaatgtag tcccagtagt caacgtgcaa
     5161 agatagcgcc agaacttgtt cggcagtgaa tcgacgcggc agttccccaa gccagcgatc
     5221 agcgggcgga cagctgctac acccttcgga agtgtaaagc tcgaccagcg ccacagtatg
     5281 tgccgggctt tttgcgctgc agctcgcggc aagcgaagcg gtcatcggca gggcgaggct
     5341 gagcgctacg gcaactttgg aagctatggt caggtgcgac acaggaatct cctgaatgag
     5401 tgtgtgcgga ttagtcgtgc gagttgcgga atacttacag cgaatcgtgt tcgcgcgggt
     5461 gtcgtggaga gacacgcatc gcttccatac agcaggtttc tttacctaaa gtagcgaaat
     5521 ccgccgtcgt cggcgtcatt cccgtgaccc gcgcccagat gcgaccgcaa ccagcctcaa
     5581 gcagcatact tgtggttacc gaaactatgt agtatccggg ccacgactgt gctttaatcg
     5641 gcagacaaag actccaattg aacgctttgc ttacctgatc ctacggtaga actgtagccg
     5701 agcaggtgcg ttgcactgaa cactggagcg catggatatt gtgccaacaa cgctgggagg
     5761 atgccgtgat cgtcgtgccg acatttcctg gatacctcct ttgcctggct tgctcggtgc
     5821 gggggcttcg tgttttttca gacgccgatc agtatcctgc agaaccggct cagttcttgg
     5881 gtttgagccg gtggggccgg gccatcaggc ctcaaattgc agtacgtcgg gtgatgtgtt
     5941 aaaaggctca cttgcttcta ccgattcaga tcgacctcgt tgaccatggt tttctctttc
     6001 ttcagtaaaa aaacgcagga aaaaaagatg gtcgccaggc cggctgtcat tcctcgtgcc
     6061 aaagaagcgc ctgaagttgc tttgccgccc gctgaagaca agcttgattc ttccgctgga
     6121 tcacctcagt ccggcacggt tcccccaccg gaagctgcca gcgatgactt gtcatcgctc
     6181 gatttcgtca agtctgattt cagtgaattt gatttcagcg aagtctctcc ggatttccag
     6241 atcgaacaag atgtcgaccc tgtggatgcc gaggccgagg aagcggcgat cctctatgcc
     6301 aatggccagg acgaagcggc acgttcagtg ctggagaatg ctgttcgcat ccatcacttt
     6361 ggtcccggag agcgtttgtg gatgatgctt ttcgacctct ataagctgac cggtcagaag
     6421 gcggccttcg atgcgctggg aatcgactac gcgcggtcat ttgaaaaatc acctccagca
     6481 tggcgcgaca agtccacggg tcaggcaaag gttagcgagg cggttgccgg gagtctgcta
     6541 ttcaagggcg atttgacggg ggaaaacgac ggcgcctttg aagcagttcg ccagtcgctc
     6601 gcaaagaatg tcaggctccg tctcgatatg tcgagagtga gtcaaattga tgcagtgggc
     6661 tgcggtctcc tgctgacctt gttgcagcag gcaagaaagt cccggcgtga agtggaattg
     6721 ctgggccgcg acgctctggc tgaaatgctt gaacagcgta tcgtggccgg gagtgcagag
     6781 gacagcgagt gctggctgct gcttctggag ctttgtcagt tgcaggggcg gaacgaggct
     6841 ttcgaggagg tggcaatcaa ctatgccgtg actttcgagg tctctccacc ctcctgggaa
     6901 tccagtcggg tagccgcccc cgagccggcg aaacaaatgg cagctgacgt tcctggcgac
     6961 gatggcgtcc aggacgccta tgtgttgcgt ggggatgtca aatcatcgcg ttttggggat
     7021 ctgacggcgc atgccgattt gcatgatcct gtactcattg attgtgcgcc gctcgtgcgc
     7081 ctggatttca tcagtgccgg cgcgcttctt aatgtgctgt cgactatccg gcaaaccggc
     7141 aagcagatcg tgtttcgtca tccgaatcat cttattgccg aattatttcg cgttatcgga
     7201 ttgcggacag tcgccgccat catttttgca aaacactagg gtttttacat ggagcaatac
     7261 cacggcacca caatcctgtc ggtgcgacga ggcaagcggg ttgccatggg cggtgacggg
     7321 caggttacgc tcggcaacat cgtcattaag gcgggcgcac gcaaggtcag gcggattcac
     7381 aagggtgata tccttgccgg ctttgctggc ggcactgccg atgctttcac gctctttgag
     7441 cgtttcgagg ccaagctcga caaacaccag ggcaatctaa tgcgctcagc tgtcgagctt
     7501 gccaaggact ggcgcagtga ccgggctttg cgccggcttg aggcgatgct ggccgtcgcg
     7561 gaccatgaaa attcactggt gatcaccggt aatggcgatg tgctggaacc agagcaaggc
     7621 atagtcgcca taggatccgg cggcgcgttc gctcaatctg cggcacgcgc cctgatcgaa
     7681 aacaccgagc ttgatccggc cgagattatc cgcaagtcgc tcgaaatcgc gggcgacctg
     7741 tgtatctaca ccaatcacaa gttaaccatc gagacgcttg actgagtatc gcccactcgc
     7801 attggtttgc catagcgcat ggccgacgag tgcatgaggg cctcgaagcc atgtctgaca
     7861 ccgcaggatt accatgtcac aaatgacccc gcaggaaatt gttcgagaac ttgataagca
     7921 tatcgttggc caggcaaaag ccaaaaaggc cgtcgcgatt gcactgcgca atcgttggcg
     7981 gcgagcgcag gtgaatgaac ctttgcgcca ggaaatcacg ccgaagaaca ttctgatgat
     8041 cgggccgacc ggcgtcggaa aaacggagat agcgcgccga ctggctcggc tggccaatgc
     8101 gccgttcatc aagatcgagg ccaccaagtt caccgaggtg ggttatgtcg gacgcgacgt
     8161 tgaaacaatc attcgcgacc tcgccgaaat tgccataaag aacggccggg atatggccat
     8221 gagcggtgtt cgtgagcgcg cggcggatgc tgccgaggag cgcattctgg atgccttgct
     8281 tcctccggcg cgcggaaatt ttttcaacga tacacaagcg gcagaagaga acgccacgcg
     8341 tcagaagttt cgcaagaagc tgcgtgaagg gactctggat gacaaggaaa ttgaaatcga
     8401 agtggccggt ccggcgatgc aggccgagat atttgcccca cccggaatgg aagagctgac
     8461 ttcgcagatt cagggcatgt ttcagaacat gggcggggct cgcaagaaat cgagaaaact
     8521 gaagatcagc gaagcgcgca agctcttgat cgacgatgag gcgggcaagc tggtcaacga
     8581 tgaagaggtc aaactcaatg ccgtgcgcga tgtcgagcag aacggtatcg tttttcttga
     8641 cgagatcgac aagattacgt cgcgtagcga cgctcatggc gccgatgttt cgcgacaggg
     8701 ggtgcagcgc gatttgctgc cactggttga agggacgacg gtatcgacga aatacggcat
     8761 gatccgtacg gatcatgtgc tgttcattgc cagcggagca tttcatctgg ccaagccctc
     8821 ggacctgata ccggagttgc aggggcgctt cccgatccgc gttgaactgg attccctgct
     8881 ggttgccgat ttcgagcaaa tcctgacgca gacagatgcc tgcctgacca tgcagtatca
     8941 agccttgctg gcgaccgagg gggtaacgct tgaatttcag ccggatggca tcaggcggct
     9001 ggccgaggtc gcctggtcag tcaacgagcg gacggagaat atcggtgcgc gccgactcta
     9061 taccgtcatg gagcgcttgc tggaagaagt atcgttcagt gccggaagcg cgggtgagga
     9121 aacgatcagc atcaatgctg aatatgttga tgggcggctg aacgaactgg ccaagaatga
     9181 agacctttcg cgatacgtgc tttaaaccca gtcgcgcggc gggagtatct cgtaaagcag
     9241 cgcttccggc gtgcccgctt ccggcgccca gtcgtaacgc catttgacga ccggtggcag
     9301 cgacatcaga atcgattcag tgcgtccgcc ggattgcaga ccgaacagcg tgccgcgatc
     9361 ccataccaga ttgaattcga cgtaccttcc ccgacggtag gcctgaaaat cccgttcacg
     9421 ttcgccgtac ggggtctcct ggcgtttctc gatgatcggc aggtaggctg cagaaaatgc
     9481 gtcgccgact gcgcgtgtca gtgcgaaaca ccgcgcatcc ccaccctcat tcagatcatc
     9541 gaagaagaca ccaccgacgc cgcgcggttc gttgcgatgc ttcaggaaaa agtactcgtc
     9601 gcaccacttc ttgtatcgcg catgaacgtc atcgccaaaa ggcgtcagcg cagacttgca
     9661 ggtggcatga aagtgacgaa tgtcgtcttc aaaggggtag tagggggtta aatccatgcc
     9721 gccgccaaac caccaaaggg tcgtcgtgtt gctgttggtt ctgccggcat ctgcacctgg
     9781 agagtcggca ttgcgctcgc cgccgggatg tgccagcaga cagcgcacgt tcatgtgcgc
     9841 cgtcgggcag taagggtttt ccggatgcag aaccagtgat acgcccattg cctcgaacgc
     9901 acggccagcc agttcaggtc gtttggcagt agccgaagca ggcagtgccg atccggtcac
     9961 gtgcgagaaa gataccccgc cgcgttcgaa tatgcggccg ccctcgatga tgcacgtcag
    10021 cccatggccg ccgagtggct ggccagcttc cttctgccat gtgtcggcta ggaaacgccc
    10081 tccgttctcg ccttccaggg cttcggcggc tgcgacaatg cgtgactgca gaccgacgaa
    10141 gtaggtcttg agggcgctgc tatcgatcat cgcggtattg cacgatgtcc gatatcgcga
    10201 cggtattgca tgccgtcgaa gtgaattcca acgattgcat cgtaagcctg cttctgcgcc
    10261 tgcttgacgt tgtcgcccag ggcggtgacg cacagtaccc taccacccga agtaaggatc
    10321 ctgccatcct tttcctgggt tcccgcatgg aacacgtgta cgtcatcact ctcgtccggc
    10381 aagccgctga tgacgtcgcc ctggcgcggc gtgccggggt agttggctgc cgccagcacg
    10441 acgcctagcg caacgcggcg atcccattcg gcatcgatct ggtcgagttt gccgacaacc
    10501 gcgtgctcaa gcagcgtgag gaagttggac ttgagacgca tcatgatggg ctgtgcttcc
    10561 gggtcgccca tgcggcagtt gaattccacc gttttcaccg aaccgtcctt gccaatcatc
    10621 agaccggcat aaaggaagcc ggtgtagggc gtgccatcgg cggccatacc gcgcacggtc
    10681 ggcaggatga tttcgcgcat ggccttggcg tggatagtcg gagtgaccac gggggcgggc
    10741 gagtaggcgc ccatgccccc ggtgttgggg ccggtatcac cgtcgccgat gcgtttgtga
    10801 tcctggctgg atgcgagcgg caacacgttc ttgccatcaa ccatgacgat gaagctggct
    10861 tcttcgccgt ccaggaattc ttcgatgacg atgcgcgaac cactcttgtc gcctgtcatg
    10921 ttcgatttgt tgccggggag catgctgtcg atggcgctgt gtgcttcgcc aagcgtcatc
    10981 gcaacaacga cgcccttgcc ggcagccagc ccatcagcct tgatgacgat gggcgccccg
    11041 tgcttgtcga caaaggcgtg ggcagcgacg ctatcggaaa atgtctcgaa ggccgctgtc
    11101 gggatgttgt gccgcgccat gaagcgcttg gcaaaatcct ttgagctttc gagttgagcg
    11161 gcttctctgg ttggcccgaa aatcttcagg ccgcgagccc gaaaaatatt gacgaccccg
    11221 gcggcgagcg gtgcttccgg accaacgacg gtgagatgaa tgccttcttt ctcagcaaag
    11281 tcggccagcg ctannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccttgg
    11401 tggtgaaatc atcaatgtct gaagtgacgg gcgccggtga acaccatcgc caatccgtgt
    11461 tcgttcgccg cagcgatgac ttcgtcgtcc cgcatcgaac cgccaggctg aatgaccgat
    11521 ttggcgccgg cttcggcgag tacatcaaga ccgtcgcgga aaggaaagaa ggcgtccgag
    11581 gccacacagg agcctgccag tacgagaccg gcgttttgtg ccttggacgc ggcgatgcgt
    11641 gttgaatcaa cgcggctcat ctggccggca ccgaccccga gcgtcatgcc gccgccgcag
    11701 aagacgatgg cgtttgattt gacgaacttg gcaacacgcc aggcaaaaag caggtcttcg
    11761 atctgcgcac tggttggtgc ggcatgggtt accaccttga ggtcggaggc ctgtacattg
    11821 aaggtgtccg gggtctgcac caacaagcca ccacccacgc gtttgaactc gaacggtggg
    11881 caattattgg acagcggcaa ctccagtacc ctgagattct gcttggatgc gagcatttcc
    11941 ttggcggccg gtgtgtaggc tggcgcgatc agtacctcga caaagtgctt gcgctcgttc
    12001 atggccttga tgacatcggt gtcgacggtg ccgttgaagg cgatgatgcc gccgaaagca
    12061 gagcttgaat ccgtcttgaa ggccttttcg taggccgaca acagcgtgct gtcgatcgcc
    12121 acaccgcaag ggttggcgtg tttgatgatg acgcaagccg cgctgttggc acctccggtg
    12181 aacgtcttta cgcactccca tgcggcatct gcgtcggcga tgttgttgta cgagagttcc
    12241 ttgccctgca attgcttata ggccgcgatg gaaccgggtg tcggtatcgg gtcgcggtag
    12301 aaggctgcct gctgatgcgg gttctcaccg tagcgtagcg tttcgacaca gtcgaaggcg
    12361 agttgaagtt tgtccggaaa ctgcttcggt ttgttggttt cgtcgagagc ggtcagccag
    12421 ttggcgatcg tcgcgtcgta tcgcgccgtg tgcgtgaaag ccttcttggc cagaccgaaa
    12481 cgcgtagcca gggacagtgc gccatcgttg gccagcattt ctgctagcag cgcggtgtag
    12541 tcttcagggt cggtgacaac ggcgactccc gtgtagttct ttgccgccga gcggaccatc
    12601 gccgggccgc cgatatcaat gttttcgata gcgtcttcga gcgtgacgtc gggtttggca
    12661 attgtttcgc ggaaagggta gagattgacg caaaccagat cgatggtcgg aatatcgtgc
    12721 cgcgcgatgg tcgcgaggtg ctcgggaaga tctcggcgag caagaatgcc gccgtgcact
    12781 ttcggatgca gggttttgac gcggccgtcc agcatttccg gaaatccggt gtagtcacca
    12841 atctcgcaga catcgagacc ggcaccgcgc agttgtttgg ccgtgccgcc cgtggacagg
    12901 agtttgacac cctgagcggc aagtccttga gcaaattcaa gggcgccgcg tttgtcggaa
    12961 aggctgatca gggcttgacg gattttcatg aagagttctc gaaataatgg gtttgaacga
    13021 aaagacgctg aagcgccgag atgacgcaat atgaggaaga acgactaaat cgaagtcaag
    13081 tcaaaccagc ttgtagtcga cgagtttctt gcgtagcgtg ttgcgattga ttccgagcat
    13141 cgccgccgct tgtgtctggt tacctccagc ctgcttgatg atgacctcaa acatcgggcg
    13201 ttcgacgctt ttcagtacca tgtcatagat gggcgcgggc atctcgccat ccagattctt
    13261 gaagtaggtt tccagtgcgc tgtatatgca gctcgaaata tcgttgtgtt ctttcatgcg
    13321 gcgagttcct cccagtcatt cttatgctct gtctggatat agttcaggtg ttcattgtgt
    13381 tcggcaagcc cgaagaagaa atcattgaca gcctgttgtt gctgcctgat gtcgggtagt
    13441 tgattcatgc tgtggcggaa agctgcagaa ccgaccagac ccttggtgta ccaggagata
    13501 tgcttgcgtg ctacgcgtac tccggtttcg attccataga attcgtagag atcttcaagg
    13561 tgtgccagca ggatggtgtg gatctcgttt acacgcggtg gcatcaggtg cgtgccggtc
    13621 ttcaggaagt gttcgatttc gcgaaaaagc cagggccgac cctgagcagc gcgcccgatc
    13681 atcagcgcgt cggcgccggt cgcgtcaagg acgtatttcg ccttttccgg agagttgata
    13741 tcgccgttgg caatgatcgg gatgcatgcc tcgcgcttca ccgcggcgat ggtctcgtac
    13801 tcggcttgcc cggaatagcc gcaggcacgc gtccggccgt gcatcgccag ggcgcgaatg
    13861 ccggattgct cggcaatttt cagaattgac agcgcattgc gtgagctttt gtcccagccc
    13921 gtgcggattt tcagggtgac tggcgtttca ggcacggcat tcacgacgga ttcgagaatt
    13981 cggccgacca gtgattcatc tttgagcagg gccgagccgg ccatcacatt gcagatcttc
    14041 ttggccgggc aacccatgtt gatatcgatg atctgcgcgc cgcgatcgac gttatgtcgt
    14101 gcggcatcgg ccatcatcgc gggcgcagcg cctgcaatct ggaccgaaat cggttcgact
    14161 tcgccatcgt ggttggcgcg ccttttcgtc ttggcgctgc cgtagagcag ggaattcgat
    14221 gtgaccatct cggacacggc cagtccggcg cccaacttct tgcacaattg gcggaaaggg
    14281 cggtcggtca cgccagccat cggggcaacg aacagattgt ttcgcagtgt aaagccgacg
    14341 aattccatgg cggtggagtg ttgagggtgt ggctggggaa gtgggaattt taacccgaat
    14401 gctggctgag aaatagttga tttctgaatt gatgccatac cctgacctga gtcgcctacg
    14461 gccacgccct cgcaccccag atcatgcgct tggcgacgaa gtggcgcagc ggcggataga
    14521 ggtcaagtgc cagtagaccg atgccacgag cgaagctgat cggcgccaga tcgttggaaa
    14581 aaaggcgaac gataccatca gtgaaagcga tacttccaca ccgatcaaac tggcggcctt
    14641 tggcgtaggt cgccaacgct gtcggatttc cggcatcgct ttggtcgaac agcgattcgg
    14701 caagttccca ggcatcgcgc agaccaagat tgaagccctg acctgacacg gggtgcaggg
    14761 attgtgcggt attcccgatc caaacctgcc ggcgctgcgc gagctgtgtc cgacaacgca
    14821 gggcgagcgg aaagccggcg cgtgggccgg ttgagacaaa ctcgaggcgt ttgccaaact
    14881 gctcctgtag tgcggcgagg aagttttcgt cattgagctt gagcaatcgg gcggccttgg
    14941 cgggtcgcac ggtgaacact accgagtaat cgcgaccgag cggcaacagg gcgagcggtc
    15001 cgtcgggtgt aaaacgctcc caggcacggt tgttgtgtcc cgtagccgga cgaacttcgg
    15061 cgacgatggc gtgttgatcg taatcccgaa ccgtgatttc agggccacgc tccggtgtgc
    15121 cctcggcgtg tacgatgagc ttggccgtga tacggtgtat ggtgccttcg cgcgtaaatg
    15181 tcagtgtggt ctcttcgcgg cctgtttcaa ttgtgtcgac gctgcattgg tcgatcagtt
    15241 gctccggttt gatgcgggcg gcgagggctg cggcaagatc gcggtagcgc atgacgtaac
    15301 cgagtgccgg tgtgccgtaa tccttggcag aaagcatggt gcgtccgaat ccgccgcgtt
    15361 gcgaaacatg gatctcgcga atcggggtgc cagccattgc gttccatgca ttcagacgct
    15421 cgagcaattg cctgctgcca tgcgagagcg ccagcgcgcg cgggtcatca atccacgcgc
    15481 cgcgttggcg actgtcgatg agaattaccc gctgcctgct gccggccagc gcgagcgcga
    15541 gcgccatgcc gaccgggccg gctccgatga tcagtacgtc cgcgtcatat ggaggggaaa
    15601 gcgccgggga cgtagcgcct gcggggtgct cagtcacggc gcatcacctc ttcaatctcg
    15661 gcaatcgatt ttggcgcgct tgtgtaaact tcgcagccgt cagtggtgac gaatacatca
    15721 tcctcgatgc ggatgccgat gccatgcagg tgctcgggta ccgtggcgcc ggggcggata
    15781 tagaggccgg gttctaccgt cagcgccatg ccggcctgca gcaccaccca atcgtcccca
    15841 ctcttgtatt cgcctgcgtc atgcacgtcg agaccaagcc agtgcccggt gcggtgcatg
    15901 taaaagggct tataggtttc gctttcgatg atgccgtcca cactaccggc aagcaattgg
    15961 agatcaatca ttccttgcac aagtacgcgc agcgcagcgt catggtaagc gatgaaaggt
    16021 gctccaggct tgattgcggc gatcgccgca gtctgtgcag caagaacgat ctcatacgca
    16081 tcacgggtta cgccgctgaa cctgccgcta accgggaagg tccgtgtgat gtcggaagca
    16141 tagccttcca gctcacagcc ggcatcgatg agaaccaggg cgtgctcgga gagcagccga
    16201 ttgttttcaa tatagtgcag gatgcacgcg ttatggccgc cggcaacaat gggtgaatag
    16261 gcgtggccgt cggcgccgcg tttgcggaac tcgtaaccaa gctcggcttc gagctcgtat
    16321 tcggccatgc cggggcggca ggtgcgcatg gcacgggcat gacctgccgc ggcgatcgcg
    16381 gcggcgcggc gcatgatatc cgtttcgtgc gcatccttga tcagtcgcat tcggtcgagt
    16441 ggttcacgca gatcgcgtat ctctccaggt gcacgcttgc cggcgcggct ttgtgcgcgg
    16501 accgtgttca gcgcagaaat aacatcggta tccacagcag cctcatggcc aaatgcgtac
    16561 caaagcctgc tgcgactggc gatcaggtcg ggaagcctgt gttcgaactc ggcgaaagca
    16621 tacgcctcgt caaagccgaa ggcatcgcac acggcctcgg gaccatagcg gaaaccgtcc
    16681 cagacttcgc gctcctcatt cctttcgcga cagaagatga tcgatcgcga gacgggttgc
    16741 gaatcattgc cacccagcag aacgatgatc gcctcgggct cgggaaagcc ggagagatac
    16801 cagaagtagc tgtcgaatcg ataggggtgg tgtgaatcgc gattgcgtac gcgttccggt
    16861 gcggtcggga tgatggccac gccatcgcca atctgttcca gcagtgcgct acgtcgcgtc
    16921 gaatacggtt cgtgtttcat gataaagcga actcctttgc cttcagttcc cgatcgagtt
    16981 cggcaaggcg ttcgggtgtt ccaacgtcga cccagatacc ggggtggcgt tcgccggtga
    17041 cgagaccctt ggcgattccg gcgtcgagca gaggacgcat tttcatgacg gcgccggcgg
    17101 gcacagcggc gaaaaaatcg ggtgaaaaga ttccagtacc cgcgtaggtc agcgtcggcc
    17161 ctgtgctctc gccggcatac ctgactgttt cgccgtcgag gctaaaatcg ccgccggcat
    17221 gctgcggtgg attgtcgacc agaacaagat gggccgggtg gccagtgagc gcgtgcatgc
    17281 gcttgtgcgc ttgcgaaaaa tcccagtcgc accacacatc gccattgacc acgagaaacg
    17341 gttggctgcc aaggaacggc aatgcctggg cgataccgcc tgcggtttcc agagcgccgg
    17401 cgggttctgg cgagtaaagg atgttcaggc cgaagcggtt gccgttgccg agcgtggctt
    17461 cgatctgctc gccgagatgt gaatgattga ttacgatgtc cctgaaaccg ctggccgcca
    17521 gccgttcaag atgccagaca atgagtggct ttccgccggc gtgaagcaag ggcttgggcg
    17581 tggtgtcggt aagtggccgc atacgctcac cgcggcctgc ggcaagaatc atggccttca
    17641 ttgcgtatgt catttcactg cctcggcgct cgcattggcc ccatgtatcg gatacgcatt
    17701 tatcatcaaa aagtatagcc tgcaacgacc tgccgatttt cgagttgatc cagaaggcgg
    17761 gccagcggac gcaattcgag gtagcgctcg caggtctgcc gcaggtagct caatacgcgg
    17821 ggcatgtctt tcagataact ctccttgcca tcgcgatggc ataggcgggc gaagatgccg
    17881 agcaccttga gttggcgctg tgcccccatc cattcgtaat cacggtaaaa atcgtggaag
    17941 tcggcatgta ccggcagccc ggccttgcgc gccatttccc agtagcgaat gacgaaatcg
    18001 agctcctgtg cttcttccca gtggatgtaa gcgtcgcggt agagcgaaac aagatcgtag
    18061 gtgatcggtc cgtagaccgc atcctgaaag tcgatgatgc cgggattttc gtgaatgctg
    18121 ctggcccttg cgcttgtccc attggaaccc gccatcccgc aaaccatcaa gtttcgcgag
    18181 tgataatcac ggtgtacata gacccgaggt tgcgcgagat tgttagcgag aatcctatcg
    18241 aataccgttc gcaacgtggt ctgctgatcc acgtcgagct tggcgccgag atggcgcgca
    18301 acataccagt cggggaagag ttcgagctcg cgctcaagca gcgcccgatc atagtctggc
    18361 agcacgccgg ggcggctggc agtctgaatg gtgaccaatg cagcattggc gtcgcgatag
    18421 agccggggtg ccgtgtccaa attcagcacg tcgagatagg tggcactgcc gaggtcggaa
    18481 aggagcagaa aaccctgatc cagatcctcg gcatagaccg tcggaacatg caccccggct
    18541 gcactgaaaa gtgcggccac tttcaggaac gggcggcaat cctcgcgttc gggtggtgcg
    18601 tccatgacga tgcgggtgtt cccgtcaggc aggctgacac ggaaatagcg ccggaaactg
    18661 gcatcagccg acgccggttc gacgaggaag tccttatcgg ggtactggct tgcgaacagc
    18721 ttgtcaagcc agctacgcaa cagagcgcta cgcggcatgc cgctactcct tgagctatgg
    18781 tgctaaaatg cgtcgatttt atcactcgct ctattggccg ctgcaccgac ggaagcaaat
    18841 agggtttgca atgcttttta ctgatatttc cggatgaatt ttcccgcccg gcgcaagtca
    18901 cgcgcgctgc tcatttgcta cggttttgcg tgcttgcttt tcggcggtca taccgtcgcg
    18961 caggagcagg ctcttgcgcc gctacgcgtc aatccggcct tgctcgggct gcccccgatc
    19021 aagccggcag aagcgcctgc gatcgcaccc gtagcccctc cggcaacggc cgacaagggt
    19081 ccggaagtca aacccgtaca ggctgtcgtg gttgagccga aagcagttga tagagcccct
    19141 gaagccagcg agtccgccag ggccagaaag gacacttcgc tggagactcc gtcgcatcag
    19201 ccgcgggggg aggggcaaca agctgctccg gttgcgccgg tgccgcaaat ggagaaacaa
    19261 ccgcgatccg aaccgcaggc gcctacggtc gcgcaagtac agtcggccaa gccagtcgct
    19321 cggcaacagg ctcagcccgc ttccaggaag ccagccgaaa cagacaaatc atggttccag
    19381 cgagtctgga gtccggtggc caatgcctat aacaatggcg cacttgaatt ctatctgccg
    19441 ttgaaaacct atcattctcg aagcacgtat tcccaagagc aaatcgactc ttaccaggaa
    19501 aaccctctcg gcttcggtat cggccgtggc ttgtataacg aaaaagggaa ctgggaaggc
    19561 gtctttgcca tggcgttcca ggattcacac ttcaagcctt cgtataccgg gggttaccaa
    19621 tggaaagcaa tctggcgacc ggccgaggat gttcgtctgg gattgggcta tcttgcaggt
    19681 ttgatgtcgc gctcggacat cctgagctat gtgccttttc caattgtctt tccagtggcc
    19741 tccgtggctt acaagaactt cagtctggaa ggaacgtaca ttcccccggg tggcgacaat
    19801 gccggcaatg tcctctttat ctgggccaag tgggaaatgg gcaagaccgg tgaagcaatc
    19861 ggtacgccgg cgcagccggc gccgccaact ccgacggaaa tggccaatgc atcattcggt
    19921 ccttccacgc cggttaccag gcagcgtgtg ccgtatggcc ccgtccttga gccgggctcg
    19981 accccacgtc tggtctcctc atctgaaacc gtgccaccag tggtgcgggc cacaggtcgc
    20041 cgcgacgagg aggaagttcc cgaccccctg ccgtccctcg ttttgcaatc ctcaagaagc
    20101 atggtgacgc cgcccaaaga cagcgcggta tcgcgaccgg tgttcctcag cgcataccgt
    20161 atggggggtg acattgatcg ggagttcaat gcggaagggg aggccgaatt gcgcaagatt
    20221 ggcaccgtcg tcaatgcgga tcgattgacc tactggccga ttgacgatga aatcgaggca
    20281 gagggtaatg cgcgacttga acagggcgag gatctgatta ccggcccgaa gatgcgcttg
    20341 aaacttgagg accaggtcgg cttctttgag cagccttcct atacgatcaa acgtcagccg
    20401 tcgtccggag gaaaagccaa ggcagaaacg gaatccacgg cactcagtgc tgagcagcaa
    20461 gatgaagatt catggcttac ttcgggtttt gcctcgccgc aggtgctaga catcaagccg
    20521 gggcaaacga gctttgacta ctctaagcag agccctcgga gggcgaccgt agcgcgggga
    20581 gaagcggatc gtatcgattt cgagggcgag aaccaggtcc ggatgaccaa tgccacctac
    20641 acgacatgcg aaccgggcaa tgatgactgg tatgccaagg caggcagtct caaactcgat
    20701 tatgaccgcg aggtagccga gggtaccgac ggtacggtgt actttctagg tacgccgatt
    20761 ctctattcgc catggctatc tttctcgctc aacaaccaac ggaaatcggg cttcctcgcg
    20821 ccgacattcg gtacgagcag taacaggggc atcgaacttg cgatgcccta ctactggaat
    20881 attgcgccga acatggatgc aacaattgcg ccacgcgtga tgaccaagcg tggcgttcaa
    20941 ttgaataacg aattccgata tctcaacacg gcgtttggcg gcacgtatcg aggagagctt
    21001 cacgccgagg tgttgcccaa cgaccgggag cggaacggcg agaatcgcta cggttactcg
    21061 gtgcagcata atcaggcgat ggcgaatggc ttctccggcg tgatcaacta cggcagggta
    21121 tcggatgacg attacttcac cgatctgtcc agtcagattt cccatacttc gcaagtcaat
    21181 atcctgcagc aaggcgtatt gagctatggc gggggcggat ggtggaatgc aacagccaat
    21241 ttccagcagt atcagacctt gcagcccgat cccgagaatc cgacgctgta cccttaccag
    21301 ttgttgccgc agtttacagt cactgccaga agacctgatc tgtatatgac cgataccagt
    21361 ttctttgggc agtacacaaa ctttacaatc aaagagcgca tacagaatgt cgatggcgtg
    21421 cccacgcttt accctgatgg tcagcggacc gtgctttatc cacaggttgc gctaccttac
    21481 gtgacgccgg gttggtatgt gacaccgaag atcggggtga acgtccggca ctatgagctt
    21541 tccggccagg ccgctggcac gccgggatca atcaatacca cgctgccgat tttcagcatc
    21601 gactcgggga tgaccttcga gcggccaagc aactggttcg gacgcgatta cacacagaca
    21661 ctcgagccac gcctctttta cgtgaatatt ccgtacaaga accaggagca gataccgata
    21721 ttcgattcag gtctggcgga tttcaacttc gcgcagattt tttccgagaa tcagttctcc
    21781 ggatgggacc gtatcaataa tgccaatcag ctaacggcgg cgatgacgac tcgattgatt
    21841 gagccatcat ccggaaacga gatcatgcgt gcgatgtttg ggcagcgttt ctatttcacc
    21901 cggaacaagg tggaactcaa cgcgagtaca agtaccccga cagaagatca gtcgtggcaa
    21961 aaatccgatg tcctcgcagc attcagcggt caaattctgc ccaaggtata tgcggatgca
    22021 gcttggcaat acaatctgaa tgatcgccag gtaaatcgct tttcgatggg ggggcactat
    22081 cgtccggaac cgggcaaggt tttcaatgcg gcctatcgtt acaaccgcga tccgaatgcg
    22141 ccgatcgatc aggtcgattt ttccggacag tggcccctta cgggacgctg gactgctgtt
    22201 ggccgattca atttctcgtt caaggatgat gcaaccaaca cttcgacagc cacacagaac
    22261 ggacgattga tcgaatccat cgccgggctg gagtacaacg gcggttgctg ggttgtgcgt
    22321 ggcgttgcgc agcaaatcgc ccttacccag gacaagacct ctacagggtt tttcatccag
    22381 cttgagctga atgatttttc gcgaattggt tccaatccgc tgaatcttct caaacgcaat
    22441 attcagggct acagcctgat caatcagccg gcagccgacc ctgtctttgg tgaataggag
    22501 gcgcgacaca tgacgcaatt gcttcgtacc ctggtactgt atttctgttt cgtcggactg
    22561 gctgcgtcac aatcgcccag ccgccaagtc agggaaccac taccggctga tcgaatcgtt
    22621 gccgttgtca atgacgaggt aattacgctc catgaattgc gtacgcgcct cgatcttgcg
    22681 cttaaccagc ttaagaagca gggcacccca ttgccagctc gcgatgttct agagcgtcag
    22741 atgctcgagc ggttagtgat ggacaaggtt caattgcaat tcgcacgcga gacaggtttg
    22801 cgcgttgatg atgcacagct tgagcaggcg ctgcagcgta ttgccgccgg caacaagatg
    22861 tcactttccc agtttcgcga ggcactgcaa aaagatggca tcccgttcgc gagatttcgt
    22921 gaggatattc gtgaagagat gaccatatcg cgtgtccgtg agcgagaagt cgagaacaag
    22981 atcacaatct cggaaggcga gattgacaac taccttagtg ccaattccgc caaaggcggt
    23041 gctggcgaag agtatgaagt tgcccatatc ctcctgcgcg tgcccgagtc ggcgagtccg
    23101 gagcagattc agaagctcaa ggccaaggct gatgaggtga tggggcgact gaagaagggc
    23161 gaagattttg cacagcttgc cgcagcttat tctgacgcac cggacggctt gcaaggcggc
    23221 aatctgggca tgcgtcccct ggatcgcttg ccagggagtt ttggagagtt ggtgatcaag
    23281 cttcaagtcg gtgaggtcgc gccgattctg cgaagtccaa acggattcca tatcgtcaaa
    23341 ctggtttcaa aacgaggtgg ttcggacatg ccagcagtgc agcagaccca tgcgcgacat
    23401 attctgatca aggtaaacga agtggtttcg gaatccgatg ccaggcacac gctggccggt
    23461 ttgtccgaac gaatcaagaa tggcgccagt tttgcggagc tggccagact gtattcgcag
    23521 gatgggtcgg catccaaggg tggagacctg ggctggatct accccggcga taccgtgccg
    23581 gaatttgagc gggcaatgaa caagctcaag cccggcgagg ttagccaggc agttcattcg
    23641 ccattcggat ttcacctgat cgaagtgctt gagcgacgcg tgcaggacgt ttcggccgag
    23701 cgccaacggg tgattgcgcg gcaggtgctg cgcgagcgca agatggatga agcgtatcag
    23761 gattggttgc gtcaggcgcg cgatcgcgcc tacgtggaaa tacgcatcga ggagcgttga
    23821 gccatgctgg cccggcccct cgtcgccgtc acttcaggag aacccgcagg agtcgggcct
    23881 gaaatctgtc tgcgattgat ggacggtgtt acgggtgcgc ttaatgcgga cctggtcgtt
    23941 ctaggtgatc gtcatttgat ggccgcgcgt gccgaagcca tcagttttac aggcgaattg
    24001 cgtgattggt cgccaggctt gcggccattg cccggacacc tcgatatcct tcatgttcca
    24061 ctgtctgtcc cgtcgcgtcc cggacatctt gatccagaaa attcgtctta tgtgcttcag
    24121 ttgctccagc gggcgatcga aggctgcaga acgggtgagt tttccgcgat ggttacggcc
    24181 cctgtgcaca aaggcgtgat caacgacgcg ggcattccgt tcaccggaca caccgagtat
    24241 ctggcagagc agacgcaaac tccgcgcgtt gtgatgatgc tcacgggcgg tggtctgaaa
    24301 gtcgcattgg cgacgactca cctgcctttg aaggaggtgc catcggcgtt gacgcagaat
    24361 tccattgagc agacgctgcg tatcctgcac gctgatttgg tcagaaaata cgccatcgaa
    24421 gatccgcgaa ttctggttgc cgggctcaat ccccacgccg gtgaaggtgg gtacctcggg
    24481 cgcgaggaaa tcgagatcat tgccccggtg ctggaccgct tgcgtgccga aggcatgcgg
    24541 ctgattggcc ccttgcctgc cgatacgatg ttcacgccgc cgatgctggc cctgggcgat
    24601 tgtgtattgg ccatgtacca tgatcagggg ttgacggcac tcaagtatgc gagttttgga
    24661 gccggcatca atgtgacgct tggcctgcca atcatccgta cctcggtcga tcacggtact
    24721 gccctcgaac tggccggaag cggtcgggcc gatccgggaa gtctcttcgt tgctgttgag
    24781 caagccattg agatggcgca acggcagtga cgtacctgca atacagcact cacagaattc
    24841 tgggattcgg atttctgctt tgcaggttca tggaatgagc aagcgtgtgg cgcgcaaacg
    24901 ttataattcc agcgtagccc gttggcatgg gctagcctgc tctgaagcat ttgaattcag
    24961 gtacgcggca tgaaccagca tgttccacgc aaacgttttg ggcagaattt cctgattgac
    25021 cgacaggtga tagccgctat tgtcgatgca gtcgcaccgg agcgtgacga ttgcgtcgtt
    25081 gagattggcc ctggacttgg cgccctgacc gacccattgc tgaaacttct ggatcatctg
    25141 caagtcgtcg agattgaccg cgatatcatc gcccgattga agacgcgtta tccgccagga
    25201 aaactgaccc tccacgaggg cgatgccctg gctttcgatt ttgcttcgct gtgcaaggga
    25261 tcatccgcag gtctgcgcat tgtcggcaat ctgccctaca acatctcgac gccattgctg
    25321 ttccatctgg ccggctttgc cgatcaggtc gtcgacatgc acttcatgct ccagaaggag
    25381 gttgtcgagc gtatggtcgc cgagcccggc actcccgact atgggcgttt gacggtaatg
    25441 cttcagtatc gttttgtgat ggactggcta cttgatgtgc cgccggaatc cttcgacccg
    25501 gcgccgaagg tcgactcggc cttggtgcgt ctgattcccc gtctgccggc tgacttgtcg
    25561 gcgaaagacg agtcaaggtt cgcggcgata gtgtctgccg ctttcgctca gcggcgcaag
    25621 atgctgcgca ataatttcaa gggcatattt gatgacacgg attacgggcg gctcggaatt
    25681 tcctcgaccg cccgagcgga ggaactgtcg gttgaggatt acatccgcat agccaacagc
    25741 atgcgatgaa tttgccaaga agcgacgacg gaaactgtcg tcaggattga ctcacggcgt
    25801 gaccaacggc gcaccgtagt ggcgcgccgc attctgtagc aaggcggatt ttgcaaaatt
    25861 cgttcagttt tgcttcttca tcgggcaggt acttatgccg agaagcgggt aagcaggaca
    25921 ccagccgatc agcccggtca gcaagggtac gacaccgatc cagccccaga caccgacgac
    25981 gcccattgct gcaagtgcaa tgaggactat gccgaccgca atgcgcagga tgcgatcaag
    26041 cccaccgacg ttcgctttca tgatgcttct ctccaaaggt taataaggga ggcagtctat
    26101 gcccgagtct ctgcgctgta tgtaacccag gtcacagagc ggcatggctc cgggtgttcc
    26161 gggtgtcgat gcgaggcata ttgcttcgga agacacatca aaagaaactt gaggatcccc
    26221 atgcagcctt gtttgcgtgc ttcgtgcttt acgcgttggg tactatttcc gatcaatgcc
    26281 ggccagttgt ttcaagccgt cccggtcgag caggcttatc cgttcgcgtc cgatagaaac
    26341 gaatccctgc gcggcaaagc cttttagcag tctgctgacg atttccctga cgctgcccag
    26401 ttcgtcggcc agcccttgat gtgtcgcctg aatcggctcg ctcttgccga gcagtagttt
    26461 ggccaatcgc tggtcgagtc ggtgaaaggc aacttcctcg acgagttgca tcaaatcggc
    26521 gatgcggtcg gcgagcaggt gaaagacgaa atcgcggaaa gtggcgtctc cggctatcaa
    26581 gcgttcaaaa agtgttttcg gcaggatggc tatctgcagt gctgtctcgg cgatgccgcg
    26641 tgctgagtag ggcatgtggc cgagaaggca actcgatgtg attacgcagg agccgcccgg
    26701 ttctatgcgg tagagcaaca tttcgcgtcc ggcaggcatg ctcttcacga ccttgatggt
    26761 gccggaaagt agcaagggaa aaccg
//
LOCUS       DAORPM010000003        25366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_310_length_25366_cov_11.315673, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000003 DAORPM010000000
VERSION     DAORPM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 25366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25366
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_310_length_25366_cov_11.315673"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            499..2136
                     /gene="pgi"
                     /locus_tag="PLD53_00270"
     CDS             499..2136
                     /gene="pgi"
                     /locus_tag="PLD53_00270"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRC59366.1"
                     /translation="MTITASPAWAALNAHRQAFGKTHLRELFSGDPKRVESLTLSFDG
                     IHYDFSKQRMSAETLGLLAALATEARVSDWTERMFAGERINISEGRSVLHVALRRSSG
                     PFPSSQFDVMPEVIETRRRMAEFAERLRSGKCVGHKGMPIRDVVNIGIGGSDLGPKMM
                     GQALRSVSHPALGVHYVSNLDGAQMAPLLQTLDPRTTLFIVASKTFTTQETMFNANTA
                     RDWLRANLGDELVSENHFAAVSSKPERAVEFGVSGDLVFPIWDWVGGRYSLWSAVGLA
                     LMIAIGPHAFDEMLRGAERMDEHFRTTPFERNLPVVMALIGIWNTNFLGATTNSVLPY
                     NESLKYFPSFLQQLEMESNGKSVTIGGEALDCATSPIVWGELGNNGQHAFFQLLHQGG
                     RVVPCDFIAAVRSDFPLPKHQEALLSNCFAQSAALAFGKNEGEVRTDLEKESLSPQEV
                     ANLLPHRVFAGNQPSSTLLLTSLNPGTLGALIALYEHKVFVQGAIWGINSFDQWGVEL
                     GKAVANRILPAVYDPDLDKTLDASTRGLLAFSRKHLK"
     gene            2172..3809
                     /locus_tag="PLD53_00275"
     CDS             2172..3809
                     /locus_tag="PLD53_00275"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HRC59367.1"
                     /translation="MKIISVATTPFSGQKPGTSGLRKKVKVFKQPGYLENFVQAIFDN
                     LDGQQGKTLVLGGDGRYFNDVAIQTILRMAAAAGFGRVLVGCNGILSTPAASAVIRKQ
                     GAFGGIILSASHNPGGPDDDFGIKYNLGNGGPANETFTDAVYQRTQEISAYRIVESAD
                     IDLGCLGETRIGDMLVSVIDPVADYAELLESLFDFDRIAGLLARPEFRMRFDAMHAVN
                     GPYAHAILEGRLGAPAGTVVNGTPLPDFGGGHPDPNPVYAADLVSALADAHSGLSFGA
                     ASDGDGDRNMIVGRDFIVSPSDSLAVIAANYALIPAYAKGLAGVARSMPTSCAVDRVA
                     ADLGIPCFETPTGWKYFGTLLDAGKATLCGEESAGTGSNHVREKDGLWAVLYWLNILA
                     VRDEPVEQIVREHWRRFGRNVYSRHDYEGIETAKAETLMDELRKKLPSLAGMKFEGMC
                     VKSADDFCYTDPVDGSRSDKQGVRVLLENGSRVVFRLSGTGTEGATLRVYLERYEADP
                     ARHAIPTQEALAPLVALAEKVASIVQITGRKAPDVIS"
     gene            3870..5141
                     /gene="glgC"
                     /locus_tag="PLD53_00280"
     CDS             3870..5141
                     /gene="glgC"
                     /locus_tag="PLD53_00280"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRC59368.1"
                     /translation="MIERRTFQLPGKAVALVLAGGRGSRLFELTDSRAKPAVYFGGKY
                     RIIDFAMSNCVNSNIRRIGVITQYKSHSLLRHLQRGWGFLRGEVNEFVDLMPAQQRVD
                     EDSWYRGTADAVFQNIDILENYRPAPEYVVILAGDHVYKMNYAAMLVDHVESGAECTL
                     ACIEVPRKDATGFGVIAVDASGRVTDFVEKPADPPPMPGKPGSSLCSMGIYIFNAKFL
                     YSELLRDINDPNSNHDFGRDIIPRAVQNGWAMSHSFDRSCVHAPEEGPGKEDYWRDAG
                     TIDAYWEANVDLTATEPSLNLYDRNWPIWTYQQQIPPAKFVHNQIDRHGLAIESTVSG
                     GCIISGEMNRSLLFSSCRVHSHAKVNWSVLLPDVEIGRGARLNRVIIDHGVRIPPGMV
                     IGEDADDDARRFRRTENGVTLVTRQMIERLA"
     gene            5163..6602
                     /gene="glgA"
                     /locus_tag="PLD53_00285"
     CDS             5163..6602
                     /gene="glgA"
                     /locus_tag="PLD53_00285"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HRC59369.1"
                     /translation="MRILHVAAEIYPLVKTGGLADIVASLPQALIKRGLDVRLLLPGF
                     PAILDGMIGLEPVLRFGPAFGAAVVTIRIGRLPDSGIEAYVIDAPFLYEREGNPYIDS
                     AGHDWSDNHRRFGLLGWVAAHMASGELSPGWRPDIVHAHDWHAGLVPVYIAQNPGLKT
                     ATVFTIHNLAFRGIFPMECYPELGLPMSELTPKSIEFRGKISFMKAGLVHSRKITTVS
                     PRYASEIRTPEFGCGLDEVLRERGSDLSGILNGVDYEIWNPNDSAIGKSYSAANIKGK
                     KDCKLSLQAELGLSNKAEGPLFAVVSRLTSQKGMDLLLAALPDLLKEGIQLAVLGTGD
                     ENLEAGFRRAAVDNPGMVAVHIGYDEVMSHRFMAGADVFLVPSRFEPCGLTQLYALRY
                     GTLPLVRRVGGLADTVFDVTPENLQAEKATGFVFDAATLPELLKCVRKACALYSDATT
                     WLRVQRRAMVQDFSWDDSAANYESLYRSL"
     gene            complement(6714..7103)
                     /locus_tag="PLD53_00290"
     CDS             complement(6714..7103)
                     /locus_tag="PLD53_00290"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRC59370.1"
                     /translation="MAETTTKAAPKKAATKTVAASAKPAAKKPAAKPTAASAKPAVAK
                     PAVVKAATASAKPAATAKPTVAKAATAKPAARSAKPVTISDEVRYRMIGEAAYYRAEK
                     NHFKSDPVRDWIEAESDIARLLAGGNK"
     gene            complement(7258..8802)
                     /gene="malQ"
                     /locus_tag="PLD53_00295"
     CDS             complement(7258..8802)
                     /gene="malQ"
                     /locus_tag="PLD53_00295"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRC59371.1"
                     /translation="MPFQRHSGILLHPTSLPGPHGSGDLGPSAYYFVDWLVAGSQTLW
                     QVLPLGGIGPGNSPYMSPSAFAGNELLIDLAQLRDAGWLSDADLSSDPAFNDGQVDYP
                     LMQQYRMARLRLAAEKFFADSRHNPREQFTAFCSEARNWLDDYALFMALDKEYGGCGR
                     VWQDWPSPLVRRNPEAMNEAAQTLADESNFWKFCQWCFYSQWAELKEYANKRGVEIIG
                     DMPIFVSAHSADVWANQQLFDLDDKGRPRVVAGVPPDYFSATGQHWGNPLYHWPAHEK
                     EGYRWWIERLRATLALCDIVRIDHFRGFESYWEIPASSETAVDGQWQPGPGAALFNAM
                     QNAMDNRQSQLSDVPRIIAEDLGIITPEVTALRESVGLPGMRILQFAFDGHSDNLYLP
                     HSFEANTVVYTGTHDNDTTCGWWSGLAPHEQDYVRRYLAISGEFNEWDLIRVASASVA
                     ALAIIPMQDVLGLDSTCRMNFPGHAEGSWGWRFGWHQVAPWHAERLAELTQLYGRVPK
                     RQTDIE"
     gene            complement(8807..10813)
                     /gene="glgX"
                     /locus_tag="PLD53_00300"
     CDS             complement(8807..10813)
                     /gene="glgX"
                     /locus_tag="PLD53_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRC59372.1"
                     /translation="MGTITPDTDLEEGRPWPLGAHWDGAGVNFSLFSANAERVELCIF
                     EGSTIHTLPLPKCTDQVWHGYLPGASPGLCYAFHVFGPNSPGHRFDPSRLLLDPYARE
                     LSGTFSYDPLPSFANSPKSCVTDEAFDWGDDAPPSTPWADSVLYEIHVKGATRQHPGV
                     PKSLRGTYAGLASPAMLKHFKRLGITAVNLLPVHHFLDERRLLDNGLTNYWGYNTLSF
                     FAPEPRYAARVGGQSVIAEFRSMVRSLHAAGIEVILDVVYNHTAETDEFGPTISFRGI
                     DNSSYYRLTGNDLNYYENFSGCGNTLNLAHPRVLQLVMDSLRYWVSEMHVDGFRFDLA
                     ATLTRDSGFLAAVRQDPVLARIKMIAEPWDLGPDGYHLGRFPPGWSEWNDRFRDVTRA
                     FWLTGESEPAAMAQRLAGSSEVFRYSGRAPQAGVNFITAHDGFTLRDLVSFQEKHNEL
                     NGEDNRDGHGHNISWNCGEEGVSDDPIVLQRRHHLQRALLATLFVAQGVPMLQGGDEL
                     GRTQSGNNNAYCQDNDLTWLDWEHADTELIDFSAKLIALRRRFPQLRRCNWLTGESNN
                     RGQRDIVWWHPGGREMCHDDWHSPPRGALGFVLSPAVPNDADESSQTLLVLINRDSDE
                     QTFHLPSGSWLQLCDSSAESPFARLQHQGAVSLTARSIQLLSQE"
     gene            complement(10801..12993)
                     /gene="glgB"
                     /locus_tag="PLD53_00305"
     CDS             complement(10801..12993)
                     /gene="glgB"
                     /locus_tag="PLD53_00305"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRC59373.1"
                     /translation="MLSKAEVISLCRGEHGNPFAVLGLHAGTKGRLWLRSLQPGADAV
                     FVIDAESGQELIELTQRKIEGLGDASGFFEASILGRSEPFDYRLRIVWPGGVQEIEDP
                     YCFSTVLGELDVWLLAEGSHLRPYERLGAHLREIDGVQGTAFAVWAPDAQRVSVVGDF
                     NSWDGRRHPMRLRRECGVWELFLPNVGSGARYKYEIRSQDGHLLPQKADPYGFAAELR
                     PSTASVVTELPQIASRSTVTAGNKMSAPISIYEVHLGSWRRAEGGGFPDWQRIAETLI
                     PYVVDLGFTHLELLPISEHPYDGSWGYQPLGLYAPTARFGSPQGFCDFVSACHAANLE
                     VIVDWVPAHFPTDAHGLGNFDGRPLYEHADPREGMHQDWGTLIYNFGRREVFNYLVGN
                     ALFWIEHYGVDSLRVDAVASMLYRDYSRSHGEWVPNIYGGRENIEAVHFLRRMNETIG
                     QECPSAATFAEESTAWPAVTRPPSMGGLGFNYKWNMGWMHDVLSYMALEPIYRRYHHH
                     QLTFGLLYAFTENFVLPLSHDEVVHGKGSLINKMSGDYWQKFANLRALYGFMWAHPGK
                     KLLFMGGEFAQWNEWNDAISLDWNLLDFPQHDGVRRLIRDLNQLYRKTPALYELDFEP
                     AGFEWISANDSDNSVIAFIRRGYDRSRAIMCVCNFTPVVRHNYRVGVPGPGLFRERMN
                     TDSQHYGGSDVGNAYGIANAEEIPAHQHAWSVSLTIPPLATVYFEWEQ"
     gene            complement(12995..15454)
                     /locus_tag="PLD53_00310"
     CDS             complement(12995..15454)
                     /locus_tag="PLD53_00310"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRC59374.1"
                     /translation="MPQSKKSKVDGVAFHAEIDDKLLTSVAAEPITANNKELYKALSL
                     VARDELSQRWVNTQVDDRKNKVRRIYYLSMEFLVGRTLNNALSALNLRDNAEAAFSAA
                     GGPSLTDIIECEPDAALGNGGLGRLAACFLDSMATLELPSWGYGMRYEYGMFAQSIIN
                     GGQVEHPDSWLVDGTPWEFPRPGTHFTVNFGGTSEHHGEWAEWNPAEAVEGRAFDYVI
                     PGHGTKRVSTLRLWKAGAPAQIDLNAFNSGDYARAAEFKNRFENISWVLYPNDTTPAG
                     RELRLRQEYFFVSASLQDILFHHQEEYGTVSNLAEQVAIHLNDTHPAISVAELMRLLC
                     DEHGMLWAEAWNQCRRIFSYTNHTLMPEALETWPVSLMTGLLPRHMRIIYRINQDFLD
                     GVAKRFPGDVDLLRRVSLIDDADGNPDNKRVRMAHLSIVGSHRVNGVSQLHSDLMVQT
                     IFADFAKLYPNLFDNKTNGVTPRRWLAQANPGLSALLDERIGDGWRLNLDLLQKLRPL
                     AADKGFRESFSAAKRSNKLRLADYIKRETGVILNPDSMFDVQVKRIHEYKRQLLNVLH
                     VMTRYNAILHGLADDSAPRCVIFAGKAASSYYMAKQVIRLIHDVANVVNNDPRTMDKL
                     KVVFVPNYGVSVAELIMPAADLSEQISTAGTEASGTGNMKFSLNGALTIGTEDGANIE
                     IRDNVGAENIFIFGNTTPQVEAIRKAGYDPKAIYQKNPMLNETLNKLDGGFFSPTDKR
                     RYHDIFNCLVNYGDHYLLLADYADYIATQAKVDTLYKTPDEWHRKAILNVAGMGSFSS
                     DRTIRDYAINTWDMGAFLKKV"
     gene            complement(16296..17318)
                     /locus_tag="PLD53_00315"
     CDS             complement(16296..17318)
                     /locus_tag="PLD53_00315"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRC59375.1"
                     /translation="MRRTTLSRYLIEEQREKGKINADLRLLLEVVSRACKGISIAIGK
                     GASAENGGVLGEAGSDNVQGEAQKKLDVLSNEILLDANEWGGHLAGMASEEMAEPHSI
                     PNRYPQGEYLLLFDPLDGSSNIDINVSVGTIFSVLRCPEGADPTIASSFQQPGTSQVA
                     AGYAVYGPTTLLVLTVGDGVVCFTLDREYGSFVLTHENMRIPAETKEFAINMSNARFW
                     DAPIKRYIDDLLEGSSGPRGKDFNMRWVAAMVADVHRIMTRGGVFLYPIDSRNRDKGG
                     KLRLMYEANPMAFVVEQAGGAATTGSERIMDIVPTGLHQRVSVVLGSKSEVETVERYY
                     REDTGQ"
     gene            complement(17508..18059)
                     /locus_tag="PLD53_00320"
     CDS             complement(17508..18059)
                     /locus_tag="PLD53_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRC59376.1"
                     /translation="MAITTPLCNFGWEAPEFELPDTSGKHWTLDTLRGPNGLLLMFIC
                     NHCPYVKAIIDRICRDALQVQEMGIGVAAIMSNDTDAYPEDSLANMETMARNLDFSFP
                     YLYDETQDVARNYDAVCTPDFFGFNKELQLQYRGRLDSSGRTSAGPDVRRELVEAMRQ
                     VAATGRGPAEQRASMGCSIKWRS"
     gene            18115..18873
                     /locus_tag="PLD53_00325"
     CDS             18115..18873
                     /locus_tag="PLD53_00325"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRC59377.1"
                     /translation="MSTPRFYCLEPLVIGSRYVLPDAAARHASRALRLRVGDDLVLFD
                     GAGGEYVARIAVLDRDQVSVDVMEWCDVECEAPIEVTLVQALQAGEKMDMTVQKAVEL
                     GARRIVPVTSQRSTVRLAGERAVRRVEHWRGVAIAACEQCGRNRVPEVSALQSLEQWL
                     GKSPNSGVLRLMLVPGSEQTLASIAPPAPGGKVDVLIGAEGGLAPDEVERAELAGFVS
                     IRLGPRILRTETAGLAVLAAMQCLWGDFRGMDHV"
     gene            18866..20377
                     /gene="pap"
                     /locus_tag="PLD53_00330"
     CDS             18866..20377
                     /gene="pap"
                     /locus_tag="PLD53_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HRC59378.1"
                     /translation="MFESAELGHTIDKATFRKEVPKLRADLLDAQFDLRENGQFPVVI
                     LVSGVDGSGKGETINVLYEWMDPRYLSTLAFSAPSDEERERPYMWRYWRALPPKGRAG
                     IFAGSWYSDPIRDRILDQLDKGDLDQRMDQINRFEAMLVNEGALVLKFWFHLSKDGQH
                     QRLKALEKDPRTAWRVTKESWTRLKTYDKLQEEASHVLRMTNTPWAPWIVIEGTDDRY
                     RSLTVGKVVLDALQKRLADKRDQNFPVAPPLAPKIDTLDVLTALDLTQQLERKAYKEQ
                     LAKWQGRLSELVRHPGFKMHSLIIAFEGSDAAGKGGSIRRIIAAIDARQYQIIPVAAP
                     TEEERAQPYMWRFWRHIPRTGRVAIFDRTWYGRVLVERVEGFCAEADWLRAYTEINDF
                     EHELQKDGAIVIKFWLQISDKEQLRRFKERENIEFKRFKITEEDWRNREKSEAYHTAV
                     CDMVDRTSTSTAPWTLIEANDKSFARVKILRTICERLEAALNIEKSKEKSKDK"
     gene            20382..21701
                     /gene="argA"
                     /locus_tag="PLD53_00335"
     CDS             20382..21701
                     /gene="argA"
                     /locus_tag="PLD53_00335"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HRC59379.1"
                     /translation="MHDELSSAHFVSLFRSVAPYVNLFRGKTFVIAFGGKAISGPLAR
                     TLAYDVNLLAALGVRLLLVHGARPQIEEELIEKGIRSVTHLGYRVTDAATLECVIDAV
                     GSVYLEIDALLSQGLPDTPMANSRIRVIAGNFITAKPLGVLDGVDMQYAGSVRKIDAE
                     GIRAQLGIGNIVILSCLGASPTGEIFNLAMEEVAESAAKAIAADKLIFLTDSQGVTDS
                     DGKLLDELTADSAERLIAIGDWLSPDIKRYLPCAVRTSRSGVGRVHLINYNEDGTLLR
                     ELFSRDGVGTIITRESLEILRDARPDDISGLVALIEPLEEIGALVRRSRELLEREITR
                     FSVVEHDGLIVGCAALYPYGDDQAELACVAVHPHYRRWGYGNQLIKRIETRARAVGIK
                     RLFVLTTVTSHWFKERGFAEQSVDQLPAEKQLVYNLQRRSKVFVKPV"
     gene            21780..22052
                     /locus_tag="PLD53_00340"
     CDS             21780..22052
                     /locus_tag="PLD53_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HRC59380.1"
                     /translation="MARMVTCIKLGREAEALDFPPYPGELGKRIYEEVSKEAWQQWIR
                     MQTMIINENRLNLADAAHRKYLAEQIEKHFFGSGVDQVQGYVPPNS"
     gene            complement(22124..22825)
                     /gene="phoU"
                     /locus_tag="PLD53_00345"
     CDS             complement(22124..22825)
                     /gene="phoU"
                     /locus_tag="PLD53_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRC59381.1"
                     /translation="MSDHTSKNFDLELENLRTRVLQMGGLAEEQVRKAMEGLNTGDHP
                     LLETVIRNDALINEIEIETETSCYQIIAKRQPTANDLRMIVSVLKAIADLERVGDKAR
                     KIARLGIALTHHPSAGSLNIELNHMADTALKMLRLSLDGFARLDISLVTRAMHLDSLV
                     NAEYLSVTRQLITYMMEDPRTITRSLDILSIAKAIERIGDHASSIAEYVIFMVKGLNV
                     RHATVDEVDAKIANS"
     gene            complement(22901..23572)
                     /gene="rpiA"
                     /locus_tag="PLD53_00350"
     CDS             complement(22901..23572)
                     /gene="rpiA"
                     /locus_tag="PLD53_00350"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRC59382.1"
                     /translation="MNQDELKLAKLAAARAALDYVVEEEIVGVGTGSTARLFIDELSG
                     MKNQIAGAVASSEDTRKRLEGHGIRVFDLNDISELPVYIDGADEITPGMHMLKGGGGA
                     LTREKIVAAVATRFICIVDESKKVDLLGAFPLPVEVIPMARAYVTRELTKLGGSPVLR
                     EGFVTDNGNLILDVEGLMIVDPVELETRINQIVGVVSNGLFARRPADVCLLGTSTGVQ
                     TLTSD"
     gene            23739..24530
                     /locus_tag="PLD53_00355"
     CDS             23739..24530
                     /locus_tag="PLD53_00355"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRC59383.1"
                     /translation="MPTVRSIFLSDIHLGTRACQADRLLEFLRSYVAENVFLIGDIVD
                     FWAMRRSIVWTSEQNTVVQKILKRARRGERVVFIPGNHDEALRDYIGITFGDIEVVRE
                     HVHQLADGRRFLLLHGDEFDQITRHHRWVAVLGDVMYDVLVRLNAMIAFVRRRFGMTC
                     YWSLAGYAKRRVKKALQFIFDFEDSASHAARQRGLSGIICGHIHSAVIREVGGVTYVN
                     CGDWVDSCTAIVEHLDGRLELIAWDSRTIGQVEVDVTEEEMACAS"
     gene            24518..>25366
                     /locus_tag="PLD53_00360"
     CDS             24518..>25366
                     /locus_tag="PLD53_00360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC59384.1"
                     /translation="MRVLMVSDVYFPRINGVSTAIQTYQQALRSHGVEVSLIAPDYGQ
                     PCSEPWITRVPAWRVPGDPEDRLVRWTEMHRVVAEAVRQGCDLIHVQTPFVAHYAARA
                     ASLNHGIPIVATYHTLFEEYLKHYAPLIPAPWLRGLARRFSRRQCNVLDAVIVPSRAI
                     HERLAAYGVKTPLHVLPTGISLRMPDHDNRSAFRTRFGIEASRPVALFVGRVAHEKNI
                     GFLLDVIDLARDSIPGILLVIAGEGPALPQLRQTVAHRGLQQNVQFIGYLDRTTDLPS
                     CYAAADV"
BASE COUNT         5496 a         6722 c         7354 g         5794 t
ORIGIN      
        1 agagcagcgg actgaaaatc cgcgtgtccg tggttcgatt ccgcgactgg ccaccagtac
       61 cgccaagggc ttgaccgtaa ggtcaggcct ttgttgtttg gggaccgtga tagaaacgtg
      121 accgagggaa gtgctcgaag ctcccgtatc tacagtagca aaatgccgtg tggccaccgg
      181 ttccaaagct tcacttgcta cgggtgagtc ttttcgacta cggcacatgc agcacaggca
      241 acctttgcaa caaattgaat ctatttgttt ttccgtcaga tattggcgcg acaaaacatg
      301 attggcgagc tgttgtttcg tcggaagccc cgaatctatt ccaatccgca tccagtttgt
      361 atccaaatta ttatgaattg agtatcgctg gagggtaaat atttcgcata gaatagaccc
      421 gattacgtta tgtgctgcgc ggtttggcgg ccacgacgtg gagtccacca agtaataata
      481 gtaaaggggt gttacacaat gacgatcact gcctcgcccg catgggcggc cctaaatgcc
      541 catcgccaag cgttcgggaa gacacacttg agggaattgt tttccggcga tccaaaacgc
      601 gtggaatcat tgaccctttc gttcgacggt atccactatg atttctccaa gcagcgcatg
      661 tccgccgaga cgctcggttt gcttgccgcg cttgctaccg aggcacgggt ttcagactgg
      721 acagaacgca tgtttgccgg tgagcgcatc aacatcagcg aggggcgttc agtcctgcat
      781 gttgctttga gacgttcttc cgggccgttc ccgagcagcc agtttgatgt gatgccggag
      841 gtgattgaaa cgagacggcg catggccgaa ttcgccgagc gcctgcgttc gggcaaatgc
      901 gttgggcaca agggaatgcc gattcgggat gtcgtcaata tcggtatcgg cggttcggat
      961 ctgggcccca agatgatggg ccaggctctg cgctctgtca gccatcccgc acttggggtt
     1021 cactatgtgt cgaacttgga tggggcgcag atggcgccac tgctccagac gctggatccg
     1081 cgtacgacgc tgtttattgt cgccagcaag accttcacca cacaggaaac catgttcaac
     1141 gcgaatacgg cgcgtgactg gttgcgagcg aatcttggcg acgaattggt ttcagaaaat
     1201 catttcgcag cggtctccag caaacctgag cgagcggttg agttcggtgt cagcggtgat
     1261 ctggttttcc cgatctggga ttgggttggc gggagatatt ccttgtggtc tgccgttgga
     1321 ttggcgttga tgatcgccat cgggccgcat gcgttcgacg aaatgctgcg tggagcagag
     1381 cgcatggacg agcattttcg cactacgcca ttcgagcgga atttgccggt ggtcatggcg
     1441 ctcatcggca tctggaatac caacttcctc ggcgcaacta cgaattcggt gttgccgtac
     1501 aacgagtcgc ttaagtactt cccgtcgttc ctgcaacaac ttgagatgga aagcaatggc
     1561 aagtccgtca ccattggcgg ggaagcgctt gactgtgcga catcacccat tgtctggggt
     1621 gagctgggca ataacgggca acacgccttt ttccagctcc tgcatcaggg tggacgtgtg
     1681 gtgccctgcg acttcatcgc tgccgtacgt tccgattttc cgctaccgaa gcatcaggag
     1741 gctctccttt ccaactgctt cgcgcagagt gccgctttgg cattcggcaa gaatgaaggt
     1801 gaagtacgta ccgacttgga aaaagagtcg cttagtccgc aggaggtcgc caacctgttg
     1861 ccgcaccgtg tttttgctgg caaccagcct tcatcaacct tgctgctgac cagtcttaat
     1921 ccgggtacgc tcggtgcgct tattgcgctt tacgagcaca aggtgttcgt gcaaggcgca
     1981 atctggggga ttaactcgtt tgatcaatgg ggggttgagc ttggcaaggc ggtagcaaac
     2041 cgtatcctgc ccgctgtgta tgatccggat ctggacaaga cgctcgatgc atcaacacgg
     2101 gggttgttgg ctttcagtcg caagcatctc aaataatact caatcaagcg tgattaccct
     2161 tgaggaatga catgaaaatt atctccgttg ctacgacccc tttctccggg cagaagcctg
     2221 gtacttcagg attgcgcaag aaagtcaaag tcttcaagca gcctgggtat ctcgagaatt
     2281 ttgtacaagc catttttgac aatctggacg ggcaacaggg gaaaacgctg gtccttggtg
     2341 gcgatggtcg ctatttcaac gatgtggcta tccagactat cctgcgtatg gcagccgccg
     2401 ccggatttgg ccgggttctt gtcggttgca acggtatttt gtccacgccg gctgccagtg
     2461 cggtaattcg caaacaggga gcttttggcg gcatcatctt gtcggccagt cacaacccgg
     2521 gcgggccgga tgacgacttc ggcataaaat ataacctcgg aaacggtggt ccggcgaatg
     2581 aaaccttcac tgacgccgtt taccagcgga cgcaggaaat cagcgcctat cggatcgttg
     2641 agtctgccga tatcgacttg ggctgtcttg gcgagacgcg cattggcgac atgctggtta
     2701 gcgtcatcga cccggtggcc gattatgccg agcttctcga gtcgttgttc gattttgatc
     2761 ggattgccgg gctattggct cgtcccgagt ttcgcatgcg ctttgacgcg atgcatgcgg
     2821 ttaatggacc ctacgcccac gccattcttg agggacgcct tggggcgccg gcagggaccg
     2881 tggttaacgg aacgccgctg ccggatttcg gcggcggaca ccccgaccca aacccggtct
     2941 atgccgccga tctggtttct gcgctggcgg atgcccactc cggcttgtcc tttggcgcag
     3001 cttcggacgg agatggcgat cgcaacatga tcgtggggcg cgatttcatc gtcagcccga
     3061 gcgatagcct ggccgtcatt gccgccaatt acgcactgat cccggcctat gcaaaaggac
     3121 tggccggcgt ggcacgttcc atgccaacga gttgcgcagt ggatcgcgtc gccgcagatc
     3181 tcggcatacc ctgttttgaa acgcctacag gctggaagta ctttggcact ctgctcgatg
     3241 ccggtaaagc aaccctgtgc ggtgaagaga gtgctggtac cggttccaac catgtgcgcg
     3301 agaaagacgg gttgtgggcg gtactctact ggctgaacat tcttgcggtg cgcgacgaac
     3361 cggttgagca aatcgttcgc gagcattggc gtcgatttgg tcgtaatgtt tattcgcggc
     3421 acgactacga aggcattgaa acggcgaagg cagagacgct gatggatgaa cttcggaaga
     3481 agctgccgtc gttggctgga atgaaattcg aagggatgtg tgtcaagtcc gctgacgact
     3541 tctgttacac agatcctgtt gatggttcgc gcagcgacaa gcaaggcgtc cgggtattgc
     3601 tcgaaaatgg gtcgcgcgtg gtattcaggc tgtctggcac cggcactgag ggggctacct
     3661 tgcgcgtgta tctagagcgc tacgaagcgg atccggcgcg tcacgcgata cctacgcaag
     3721 aggcgttggc gccgctcgtg gccctcgccg agaaggtggc cagcatcgtg cagattaccg
     3781 gaagaaaagc gccggatgtg atcagctgac gggtgtcgtc gcaggcagtt gttattggtg
     3841 agtatggtgg attttaggag cgtgccaaaa tgatagagag gcgaactttt cagttacccg
     3901 gcaaagcagt tgcgctggtg cttgccggtg gtcgtggaag ccgattgttc gagttaaccg
     3961 acagtcgggc aaaacccgct gtttattttg gtggcaaata tcggatcatc gattttgcca
     4021 tgtccaactg cgttaattcc aatattcgcc gtatcggggt catcactcag tacaaatcgc
     4081 acagtttgct acgccacctt cagcgtggct ggggtttttt gcgcggtgag gtcaacgagt
     4141 tcgttgacct gatgccagcc caacagcgcg ttgacgaaga ctcctggtat cgtggaacgg
     4201 ctgatgctgt tttccagaat attgatattc tggagaacta caggccagcg ccagagtatg
     4261 tggtaattct cgcgggcgat cacgtatata aaatgaacta tgcggcgatg ctggtcgacc
     4321 atgtcgagag cggggccgag tgcacgcttg cgtgcatcga agtgccgcgc aaggatgcga
     4381 ccgggtttgg agtgattgca gtggatgcca gcgggcgcgt cacggatttt gttgaaaagc
     4441 cggctgaccc gcctccaatg cctggcaagc cgggtagttc gttatgcagt atgggcattt
     4501 atatcttcaa cgcaaagttt ctttacagcg agttgctgcg cgatatcaac gatccgaact
     4561 ccaaccatga ttttggtaga gatatcattc cacgcgccgt gcaaaacggg tgggcaatgt
     4621 cgcactcctt cgatcgcagc tgtgttcacg cacctgaaga aggacccggc aaggaagatt
     4681 attggcgcga tgcggggacc atcgatgctt attgggaggc caatgtcgat ttgacggcca
     4741 ctgagccctc gttgaacctc tatgatcgga actggccgat ttggacttat cagcagcaga
     4801 ttccccctgc aaagtttgtg cacaaccaga ttgatcgtca tggactggcc atcgagtcga
     4861 cggtttcagg tggttgcatc atctcgggtg aaatgaaccg ctcattgctg ttctcatcgt
     4921 gccgtgttca ttcgcatgcc aaagttaact ggtcagtact cttgccggat gtcgaaatcg
     4981 gccggggggc aagattgaac cgtgtcatta tcgaccatgg agtgcgtatt ccgccgggga
     5041 tggtaattgg cgaagacgct gatgatgatg cgaggcgttt ccgtcgaacc gagaatggag
     5101 taaccctggt cacacgccag atgatcgaac gactggcctg acatatagcc cgctgctgga
     5161 tcatgcgtat actgcatgtc gccgccgaga tatatccctt agtcaaaacg ggtggtttgg
     5221 ccgatatcgt tgcatcgctg ccgcaggcgc ttatcaaacg cgggctggat gtccgccttc
     5281 tgttgcccgg gtttcccgcc atccttgacg gaatgatcgg gctcgagccc gtcctccggt
     5341 ttggtcccgc tttcggcgca gcagtcgtga cgattcgcat cggtcgcttg ccggacagtg
     5401 ggatagaggc ttacgtaatc gatgcgcctt tcctttatga gcgcgaaggc aatccatata
     5461 tagattctgc cgggcatgac tggagcgaca atcatcgccg ttttggcctt ctgggatggg
     5521 ttgccgcaca tatggcctcc ggcgagctct ctcccggctg gcggcctgac atagtgcatg
     5581 cgcatgactg gcatgccggt ttggtacccg tctacattgc ccagaaccct gggctgaaaa
     5641 cagcgactgt ttttaccata cacaatctgg cattccgtgg aatttttccg atggagtgtt
     5701 acccggagct gggtttgccg atgagcgagc taacgccgaa gagcatcgaa tttcgcggca
     5761 agatatcgtt tatgaaggct gggcttgtcc attccaggaa gatcacgacg gtcagtccga
     5821 ggtacgcaag cgagatccgc acacctgaat ttggttgtgg cctcgatgaa gtgttgcgcg
     5881 agcgtggtag tgatttgtcc ggtattctca acggagtgga ttacgagata tggaatccca
     5941 atgactcagc tatcggcaag agttacagtg cggccaacat caaggggaaa aaggactgca
     6001 agctttcgct acaggctgaa ctcggcctat ccaataaagc agaaggacca ctgtttgccg
     6061 tcgtcagcag gctgacttcg caaaaaggca tggatctcct cttggctgct ttgcctgacc
     6121 tgctcaaaga gggtatccag ttggctgttc tcggcacagg agatgagaat ctggaagccg
     6181 gttttcgtcg cgcagctgtg gacaatcccg gtatggttgc cgtgcatatt ggttacgacg
     6241 aagtgatgtc tcatcgtttc atggccggcg ccgatgtttt tcttgtcccg tcgcgttttg
     6301 aaccctgtgg gctgacacag ctctatgcct tgcgatacgg tacgttgcct ctcgtgcgcc
     6361 gagtgggggg gttggcagac accgtgttcg atgtgactcc agaaaacctt caggcagaga
     6421 aggcaaccgg atttgtattt gatgcagcaa cgttacccga actccttaaa tgtgttcgga
     6481 aagcttgcgc actctacagc gatgcgacca cctggcttcg ggttcagcgg cgagcaatgg
     6541 ttcaggactt ttcgtgggat gattccgcag cgaattatga atcactctac cgcagcctgt
     6601 gaaccaacgg ctagcatagg gccccgcgca taaccctcag ccggcatcct ccagcttgga
     6661 tgactctccc ccggcgcaca atcagtgccg gaggaggttc tacggcctaa tgactacttg
     6721 ttgccaccag cgagaagcct agcgatatcg ctttccgcct caatccagtc gcgtacggga
     6781 tcgctcttga agtggttctt ttcggcgcga taataggccg cttcaccgat catgcgataa
     6841 cgtacttcgt cgctgatcgt cacaggcttg gcactacgag cagcgggttt tgccgtcgca
     6901 gccttggcga cggtcggctt ggcggtcgcg gctggctttg cgcttgcagt cgcggcctta
     6961 actactgcag gcttggccac tgcgggctta gcgctagcag ccgtaggctt ggctgcgggt
     7021 ttcttggccg caggcttggc gctggcagcg acggttttgg tcgccgcttt ctttggggct
     7081 gctttggtgg ttgtctcggc catgatttta tccctataaa aaatggagaa actggttctg
     7141 aaataagagc aacgacggga ttgtactgca taagtacaat ttaaaaaact tcatcatcat
     7201 gtattctgct ttgcgaactg cctgcaagta cttgcagcag caattcgcac tgcttggcta
     7261 ctcaatgtca gtttggcgtt tcgggacacg tccgtaaagc tgtgtcagtt ctgcaaggcg
     7321 ttcggcatgc cagggggcaa cctgatgcca gccgaagcgc cagccccagg aaccttcagc
     7381 atgcccagga aagttcatgc gacaagtgga atctaggccg agtacgtcct gcatgggtat
     7441 gatcgcaagt gcggcaacgg acgccgaagc gactctgatc aggtcccatt cgttgaactc
     7501 gccactgatg gccagatagc gccgaacata atcttgttca tgaggtgcga gcccagacca
     7561 ccaaccacaa gtcgtgtcat tatcgtgggt gcccgtatag acgactgtat tggcttcgaa
     7621 gctgtgcggt aaatacagat tgtcggaatg tccgtcaaaa gcaaactgca ggattcgcat
     7681 tcccggcaga ccaaccgact cgcgcaaagc cgtaacttca ggtgtgatga ttcccagatc
     7741 ctcagcgatg attctgggca catcgctgag ttgactttgc cggttgtcca ttgcattttg
     7801 catagcattg aacagagcgg ctcctggtcc gggttgccac tgtccatcaa cggctgtttc
     7861 cgaggatgcg ggtatttccc agtacgactc gaagccgcga aagtggtcga tccgaactat
     7921 atcgcagagt gcgagagttg cacgcaagcg ctcgatccac catcgatagc cttccttctc
     7981 atgtgctggc cagtgataca ggggattgcc ccagtgttgt ccggtagcgc tgaaataatc
     8041 tgggggtacg ccggcgacaa cgcgaggccg acctttgtca tcgagatcaa ataattgctg
     8101 gtttgcccaa acgtcagcgc tgtgcgcaga aacaaatatt ggcatgtcac caatgatctc
     8161 aacaccacgt ttgttggcgt actccttgag ttccgcccat tggctgtaga agcaccattg
     8221 gcaaaacttc caaaaattcg attcgtcagc gagggtctgc gccgcttcat tcattgcctc
     8281 cggattacgg cgaaccagag gcgacggcca gtcctgccag accctcccac aaccgccgta
     8341 ttctttgtcc agggccatga atagtgcgta gtcatcaagc cagttccgag cttcagagca
     8401 gaacgcggtg aattgctctc gagggttgtg tcgggaatcg gcaaaaaact tttccgctgc
     8461 cagacgcagc cgcgccatgc ggtactgctg catcaatggg taatctacct gaccgtcatt
     8521 aaaagccggg tctgaagaca agtccgcatc agacaaccaa cccgcatcgc gcaactgagc
     8581 cagatcgatc aacaactcgt tgccagcaaa tgccgaagga ctcatatagg gcgagttgcc
     8641 tgggccgata ccgccgagag gcagaacttg ccatagggtt tggcttccgg cgaccagcca
     8701 gtcaacaaag taataggccg atggccccag atcgcccgaa ccatgcgggc cgggaagcga
     8761 tgtggggtga agcagaatgc cgctgtgcct ttgaaagggc atcgtcctat tcctgagaaa
     8821 gtagttgaat gctacgtgcg gttaatgaaa cggcaccttg atgctgcaac cgtgcgaaag
     8881 gggattcggc actgctgtcg cagagttgaa gccaggaacc tgatggcagg tgaaatgtct
     8941 gttcatcaga atcccggttg atcaggacga gcagagtttg tgagctttca tccgcatcat
     9001 tcgggactgc gggggacaat acgaagccca atgctccccg tggtggtgaa tgccaatcat
     9061 cgtgacacat ttcgcgtcca ccgggatgcc accagacaat gtcgcgctgc ccccgattgt
     9121 tactttctcc ggtcagccag ttgcaacgac gaagctgcgg gaagcgccgt cgcaaggcga
     9181 tcagtttggc cgaaaaatca attagctcgg tatcggcatg ttcccaatcg agccaggtta
     9241 agtcattgtc ctggcagtag gcgttgttgt tgccggactg tgtacggccg agttcatcac
     9301 caccttgcag catcggaact ccctgtgcga caaaaagggt cgccagcaat gctcgttgca
     9361 ggtgatggcg ccgctgaagg acgatcgggt cgtcgctaac gccttcttcc ccgcaattcc
     9421 aggagatgtt gtgcccatgt ccatcgcgat tgtcttcacc gttgagttcg ttatgttttt
     9481 cctgaaaact gaccaggtcg cgcaaggtaa aaccatcatg cgcagtgatg aagttgacgc
     9541 cagcctgtgg tgcccggcct gagtagcgga aaacctcgct agacccagcc agtcgctgag
     9601 ccatggcggc cggctcggat tcgccagtca gccagaaagc acgagtgaca tcgcggaaac
     9661 ggtcgttcca ttcgctccag cccggtggaa atctgcccag atgatagcca tcggggccga
     9721 gatcccaggg ttctgcaatc atcttgatcc ttgccagtac tggatcctgt ctgaccgcag
     9781 cgaggaagcc gctgtcacgc gtgagtgtcg ctgccagatc aaaacggaaa ccgtcgacat
     9841 gcatttcgct aacccagtag cgcagggagt ccatgaccag ttgcagaacg cgcgggtggg
     9901 cgaggttgag ggtattccca caaccgctga aattctcata gtaattcagg tcattgcctg
     9961 tcaggcggta gtagcttgag ttgtcgatgc cgcgaaaaga gatggtcggt ccgaactcgt
    10021 cggtttcagc ggtatggttg taaacaacat cgagaatgac ttcgatacct gccgcgtgca
    10081 gcgaacgcac catcgagcgg aattccgcga taacggactg gccaccaacc cgagcggcat
    10141 atcgtggttc gggtgcaaaa aacgacaggg tgttatagcc ccagtaattg gtcagtccat
    10201 tgtcgagcag gcgtcgctca tcaaggaagt ggtgcaccgg cagaaggttg acggcagtaa
    10261 taccaagtcg cttgaaatgc ttgagcatgg ctggtgaagc cagtccggca taggttccgc
    10321 gaagtgattt gggaaccccc ggatgctgtc gtgtcgcacc tttgacatgg atttcgtaaa
    10381 ggaccgaatc ggcccaggga gtcgacggag gcgcatcgtc gccccagtcg aaagcctcgt
    10441 cagtaacgca ggacttcggg ctgttcgcaa atgaaggcag cggatcgtag gaaaaagtgc
    10501 cgctcagttc tcgcgcatac gggtcgagta gcaggcgact cgggtcgaag cgatggcctg
    10561 gcgagtttgg gccgaatacg tggaaggcgt agcaaagacc ggggctggcg ccagggaggt
    10621 aaccgtgcca gacttgatcg gtgcatttgg ggagcggcag cgtatgtatc gtactgcctt
    10681 cgaagatgca taactccacc cgctcggcat tggccgagaa cagggaaaaa ttgactcccg
    10741 caccatccca atgagcccca agcggccatg gccgaccttc ttcgaggtcg gtatccgggg
    10801 ttattgttcc cattcaaaat acacggtggc caggggtggt atggtcagtg aaacggacca
    10861 tgcatgctgg tgcgccggaa tctcttcggc atttgcgata ccgtatgcgt ttccgacgtc
    10921 gcttccgccg tagtgctggg aatcagtatt catgcgctcg cggaataaac cggggccggg
    10981 aacgcccacg cggtagttgt ggcgaactac gggcgtaaag ttacagacgc acataatcgc
    11041 gcgcgatcgg tcatatccgc gacggatgaa tgcgatcacc gagttatcgg aatcgttggc
    11101 cgagatccac tcgaatccgg ctggctcgaa atcaagttcg tacagcgctg gtgtcttgcg
    11161 atagagctga ttcaagtctc tgatcagacg acgcacacca tcgtgctgcg ggaagtcgag
    11221 cagattccag tcaagcgaga tcgcatcgtt ccactcgttc cactgggcaa attcaccgcc
    11281 catgaacagc agtttcttgc ccggatgcgc ccacatgaaa ccgtacagtg cccgcagatt
    11341 ggcgaatttc tgccagtagt ctccggacat tttattgatc agcgagccct tgccatggac
    11401 gacttcatcg tgcgacaggg gcaggacgaa attctcggta aacgcgtaga gcagaccgaa
    11461 ggtgagctgg tggtggtgat agcggcgata gatcggttca agcgccatat agctcagaac
    11521 atcgtgcatc cagcccatgt tccacttgta attgaagccc agccccccca ttgacggcgg
    11581 acgagtgact gccggccaag ctgtggattc ttcggcaaag gtcgccgcac ttgggcactc
    11641 ctgcccgatt gtttcgttca tacggcgcag gaaatgaacc gcttcaatgt tctcacggcc
    11701 gccatagata ttgggtaccc actcgccgtg agaacggctg tagtcgcggt aaagcatcga
    11761 tgctaccgcg tcaacgcgca gactatcgac gccgtaatgc tcgatccaga aaagggcatt
    11821 gccgaccaag taattgaata cctcacgccg gccaaaattg tagatcagcg ttccccagtc
    11881 ctggtgcatc ccctcgcgtg gatcggcgtg ttcatacaag ggcctgccgt cgaaatttcc
    11941 gagcccgtga gcgtcggtcg gaaaatgtgc tggcacccag tcaacgataa cttcaaggtt
    12001 ggccgcgtgg cacgccgaca cgaagtcaca gaagccctgg ggagaaccaa agcgtgctgt
    12061 aggcgcatac aatccgagtg gttgataacc ccacgaaccg tcgtaggggt gctccgagat
    12121 cggcagaagt tcaaggtgcg taaaaccgag atcaacaacg taggggatca gcgtttcggc
    12181 aatgcgttgc cagtccggaa agccgccgcc ctcggctctc cgccaggaac cgaggtggac
    12241 ctcatagatc gagatcggag cgctcatttt gttgccggcg gtgactgtgg agcgcgatgc
    12301 aatctgtggc aattcggtga ctaccgaggc tgttgacggt cgcagttcgg ccgcaaagcc
    12361 ataggggtct gccttttgtg gcagcaagtg accatcctgc gaacgtattt cgtatttgta
    12421 ccgggcccct gatccgacgt tcggcaggaa cagttcccat acgccacatt cgcggcgcag
    12481 tcgcattgga tggcgccggc catcccaaga gttgaagtca ccaacgacac tcacgcgctg
    12541 ggcatcggga gcccagacag caaatgccgt tccttgcact ccatcaattt cgcgtagatg
    12601 cgcaccaaga cgctcataag ggcgcaggtg cgaaccttcc gcaagcaacc acacatcaag
    12661 ctcgccaaga accgtggaaa aacagtacgg gtcttcgatc tcttgaacgc cgcccggcca
    12721 aacaatgcga agccgatagt cgaaaggttc gctacggccg agaatggaag cctcaaaaaa
    12781 ccccgatgca tcccccaggc cttcgatttt gcgctgggtc aattcgatca attcctgccc
    12841 actttcggcg tcgatgacga aaaccgcatc cgctccaggt tgcaggctgc gcaaccacag
    12901 gcgacctttc gtaccggcgt gcagtccgag cacggcaaac ggattgccgt gttcaccacg
    12961 gcacaaggaa attacttctg ctttgctcag catatcaaac cttcttgaga aatgcgccca
    13021 tatcccaggt gttgatggcg tagtctcgaa tggtgcgatc cgaggagaag ctgcccatgc
    13081 ctgcgacgtt gagaatggct ttgcgatgcc actcgtccgg cgtcttgtag agggtatcga
    13141 ccttagcttg ggtggcgatg tagtctgcgt agtcagcaag cagcaggtaa tggtctccgt
    13201 aattcaccaa gcaattaaag atgtcgtgat atcgcctctt gtctgtgggt gaaaagaaac
    13261 cgccatcaag cttgttcagg gtttcattga gcatcggatt cttttgataa atggctttcg
    13321 gatcataccc ggccttcctg atggcttcga cttgcggcgt tgtgttgccg aagatgaaga
    13381 tgttctctgc gcccacgttg tcccgaatct cgatattggc gccatcctcg gtgccgatgg
    13441 taagcgcccc gttcaacgag aacttcatat tcccagtgcc tgaagcctcg gtaccggctg
    13501 tggatatttg ctcggacagg tcggccgccg gcatgatcaa ttccgctacc gagaccccat
    13561 aattcgggac aaatacgacc ttcaacttgt ccatggtgcg cgggtcgttg ttgaccacat
    13621 tggcgacatc gtggatcaaa cggatcacct gcttggccat gtagtacgac gatgcggcct
    13681 tgccggcaaa tattacgcaa cgtggtgccg agtcgtcggc gagtccgtgc agaatcgcgt
    13741 tgtatcgggt catgacatgc agaacattga gcagttgccg tttgtattcg tgaatacgct
    13801 tcacctgcac atcgaacatg ctgtccgggt tgagaatgac gcctgtctcg cgcttgatgt
    13861 aatccgcaag tcgcaacttg ttgctgcgct tggcggcaga aaatgattcg cggaagcctt
    13921 tgtcagctgc cagtggccgc agtttctgca gaaggtcgag gttaagccgc caaccgtcgc
    13981 caatacgctc gtcgagcaga gctgacagcc cggggttggc ctgtgccagc cagcgacgcg
    14041 gtgtgacgcc gttggtcttg ttgtcgaaca gatttgggta aagcttggca aagtcggcaa
    14101 agatcgtctg aaccatcagg tccgaatgca actgcgaaac cccattgacc cgatggctgc
    14161 caacgatgct caggtgggcc atgcgcacac gtttgttgtc tggattgcca tcggcgtcat
    14221 cgatcaaaga gacgcgacga agcaggtcga catcgccggg gaagcgcttc gcaaccccgt
    14281 cgagaaagtc ctgattgatt cggtagatga tgcgcatgtg acgtgggagc agaccggtca
    14341 tcaggcttac cggccaagtc tccagtgcct cgggcatcag ggtgtgattg gtgtaggaaa
    14401 agatgcgccg gcattgattc caagcttcgg cccagagcat gccgtgttcg tcacacaaca
    14461 atcgcatgag ctcggcgacg ctgatcgccg gatgcgtgtc gttcaagtga atggcaacct
    14521 gctcggccag attgctgacg gtgccgtact cctcctgatg atgaaacaag atatcctgaa
    14581 gcgacgcgga gacgaagaag tattcctgcc tcaggcgcag ttcgcgacca gcgggggtcg
    14641 tatcgtttgg ataaagaacc caggaaatgt tctcgaacct gttcttgaat tcggcggcgc
    14701 gcgcgtagtc gccggagttg aaggcgttga ggtcgatctg agccggcgcc cctgccttcc
    14761 agagcctgag cgtactcacc cgcttcgtgc cgtggcccgg tatcacgtag tcaaacgctc
    14821 gtccttccac ggcctcggcg ggattccatt cggcccattc accatgatgt tcggaagtgc
    14881 caccaaaatt aacggtgaag tgcgtgccgg gccgcggaaa ctcccatggg gttccgtcga
    14941 ccagccagga atcagggtgt tccacctggc cgccattgat gatcgattgc gcaaacatgc
    15001 catactcata gcgcatgcca taaccccaag atggcagttc cagcgtggcc atcgagtcga
    15061 gaaaacacgc ggccaatcgg ccgaggccgc cattgccaag cgcggcatcg ggttcgcatt
    15121 cgatgatatc tgtgagcgaa ggtcctcccg ctgccgagaa cgcagcctcc gcgttatcgc
    15181 gcagattgag tgccgagagg gcgttgttca atgtgcgtcc gaccagaaat tccatcgaca
    15241 agtagtagat gcggcgtacc ttgttcttgc gatcatctac ctgggtattt acccagcgct
    15301 gagacagctc atcgcgtgca accagcgaga gcgctttata gagttccttg ttgtttgccg
    15361 tgatgggctc ggctgcaact gatgtgagca gcttgtcatc gatttcggcg tgaaaagcta
    15421 cgccgtcaac tttggatttc ttggattgtg gcatcggtca ggtactccaa aaaggttttt
    15481 acgaccatcg gtttaggcaa ggcgggtatg gcgccaagat gaaccggaac gggggataag
    15541 cattacataa gttgcgagaa ccgccaagtg ggttgtgagc cggctacggg tcgcaggcaa
    15601 cggtgaaatg aaaatgtgtc aacaaataac cgcagatcga aatgtggacc gggttcgacc
    15661 caggtgtcgg ctagcaaaag agcgaatgtc ataattgtat cagtgccagt gtcaactgcc
    15721 ccaaaagagg gcaacaggcc cggcatcaac ctagccgatc aaggtggtgt tataagaaac
    15781 tgtttcggag gattaatgtc ggtaccgcaa gcagctgttc agctgtcgag agcctacccg
    15841 gagaagtcta gttcattcat gggccggctg gcaaacatta tgcggcatcc aacatgcgca
    15901 caccaatcag atgttgatag atattctgtg tccgtgccgc aagaaaagtt cactcgcttt
    15961 tcttgaacga gtaagttaat gctcaaatct tcggaaacgt aactgcgctt ttggggtggt
    16021 cttgctgggg gcggccattg cccgtcgttc tgcagacgac cgtattgagc atgttcaccg
    16081 gcctgataca gatactcata ggcttctcgg tatcggcagg ctgcgaagcg tactttcttt
    16141 atcggcgctt tgcggccatc tgagcggtgt tggtctcacc gcccttgtgc tggtttcgca
    16201 acttacacgc acgctggctg tacgacttcg acatacaggc gacgaactga ccttgctgcg
    16261 ggatagcaga gggatttccg tagcaacgat aaatcttact gtccggtgtc ttcccgatag
    16321 tagcgttcaa cggtttcaac ttcactcttc gagccgagaa cgaccgaaac acgctgatgc
    16381 aagccggtag gcacaatatc catgatgcgt tcggaaccgg tggttgccgc gccgcccgcc
    16441 tgctcgacga caaaggccat cgggttggct tcgtacatca gacgtaactt gccgcccttg
    16501 tcacggtttc tgctgtctat cggataaagg aatacgccgc ctcgcgtcat gattcgatgc
    16561 acgtcagcaa ccatcgcggc cacccagcgc atgttgaagt ccttgccgcg aggaccgctc
    16621 gaaccctcca gcaggtcgtc gatatagcgt ttgataggcg catcccagaa acgcgcgttc
    16681 gacatattga tggcgaattc tttggtttcg gcaggaatcc gcatgttctc gtgggtgagt
    16741 acgaaggagc cgtattcacg atcgagcgtg aaacagacga caccatcccc gacagtgagt
    16801 accagcagtg tggtcgggcc gtagacggca tatccggccg caacctggct ggttccaggt
    16861 tgctggaagg acgacgcgat tgtcgggtca gcaccttctg gacagcgcaa cacggagaaa
    16921 attgtaccga cagaaacatt gatgtcgatg ttagaggagc catccagtgg atcgaacagt
    16981 aggagatatt cgccctgcgg ataacggttc ggaatggagt gtggctcggc catttcttcc
    17041 gaggccatgc cggctaggtg ccccccccac tcgttggcat cgaggagaat ttcgttggag
    17101 agcacgtcga gctttttctg cgcttcaccc tgaacgttgt cacttccggc ttcgccgagc
    17161 acgccgccgt tttcagccga cgcgcccttg ccgatggcga ttgaaatgcc tttgcaggca
    17221 cgggaaacca cttcaagcag aaggcgcaaa tcagcgttga tcttgccttt ttcccgttgc
    17281 tcttcaatca ggtaacggct gagtgttgtg cggcgcatgg atgaatctcc gaaagttttg
    17341 gctaaaagca cgtgaagtgg gtgtgggtag ttaaccagta tactagtcaa ccataagact
    17401 gaacgtcgat tctacctcaa gtcgatcggt ttgagaatcg atttagcgaa attcgacctt
    17461 gcagcaggaa attacgaagt cgggatgcct tgttcaggag cggttcttca agatcgccat
    17521 ttgatcgagc agcccatgct tgcccgctgt tcggccgggc cgcgtccagt ggccgcaact
    17581 tgtcgcatgg cttcgaccag ttcgcgccga acatcgggcc ccgcggatgt gcgtccgctt
    17641 gagtcgaggc gaccacggta ctgcaactga agctccttgt tgaagccaaa gaaatccggc
    17701 gtacacacgg catcatagtt acgtgcgacg tcttgtgttt cgtcgtagag ataaggaaaa
    17761 gagaagtcca gattcctggc catcgtttcc atgttggcta aagaatcttc cggataggcg
    17821 tccgtgtcgt tgctcatgat ggctgcgaca ccaataccca tttcctgaac ctgcagcgca
    17881 tcgcgacaga tacggtcgat gatggccttg acatacgggc agtgattgca gatgaacatc
    17941 aacaatagac cgttgggacc acgaagcgtg tctagtgtcc agtgtttgcc tgaagtgtcg
    18001 gggagttcaa attccggggc ttcccagccg aaattgcaga gcggggtagt gatggccatg
    18061 actgtggtaa tccttatgat tacgctgtct tacgcgtgct tctataatat caccatgagt
    18121 accccacgat tctactgcct cgagcccttg gtgatcggtt cgcgctatgt tctgcctgac
    18181 gccgccgcaa ggcacgcgtc tcgggcgctg cgattgcgcg ttggggatga tctcgttttg
    18241 ttcgatggcg ccggcggtga gtatgtggca cgaatcgccg tgctcgatcg agatcaggta
    18301 agcgtggatg taatggaatg gtgtgatgtc gaatgcgagg cgccgataga ggtgacgctt
    18361 gtgcaggctc tgcaggctgg cgagaagatg gacatgactg tgcagaaagc ggtcgaattg
    18421 ggtgcgaggc gaatcgtccc ggtgacgtca cagcgcagta cggtacgtct cgctggcgaa
    18481 agggcagtgc ggcgtgttga gcactggcgt ggcgtggcca tcgcggcctg cgaacagtgc
    18541 ggacgcaacc gggtgccgga ggtgtccgca ctgcagagtt tggaacagtg gctgggcaag
    18601 tctccaaaca gcggtgtttt gcgcttgatg ttggtgcccg gatcggagca aactctggct
    18661 agcattgcac caccggcgcc gggcgggaag gtggatgtgc taatcggcgc tgaaggaggt
    18721 ttggcacctg atgaagtcga gcgggctgaa ctggccggct ttgtctccat ccggctcgga
    18781 ccgcgcatat tgagaaccga aacagcgggg ctggctgtac tggcagcgat gcagtgtctg
    18841 tggggcgatt tcaggggcat ggatcatgtt tgaatctgca gaattgggcc acacgatcga
    18901 caaggcgact ttccgcaagg aggttccgaa gctgcgcgca gatttgcttg acgcgcagtt
    18961 tgacttgcgc gagaacgggc aatttcctgt tgtgatcctg gtcagcggtg tcgatgggtc
    19021 tggcaagggt gagacgatca atgtgcttta cgaatggatg gacccacgct acctgtctac
    19081 cctcgccttt tcggcgccga gcgacgaaga acgcgagcgt ccctatatgt ggcgatactg
    19141 gcgagccctg ccacccaagg ggcgcgcggg catatttgcc gggtcttggt actcggatcc
    19201 gatccgggat cgaatcctgg atcagctcga caaaggtgac ctcgatcagc gaatggatca
    19261 gatcaatcgt ttcgaggcga tgctggtcaa tgaaggtgct ctggtgctca agttctggtt
    19321 tcacctttcc aaggatggtc agcaccagcg gttgaaggcg ctcgagaaag acccgcgaac
    19381 ggcctggcga gtgaccaagg aaagctggac gcgccttaaa acctacgaca agttgcagga
    19441 agaggccagc catgtgctgc gcatgaccaa tacgccatgg gcgccctgga tcgtcattga
    19501 gggcacagat gaccgctatc gttccttgac cgtcggcaag gtggtccttg acgctttgca
    19561 aaaacgtctg gccgacaagc gtgatcagaa cttcccggtg gccccgccac tagccccgaa
    19621 aatcgatacc ctcgacgtgc tgaccgcgct ggacctgacg cagcaattgg agcgcaaggc
    19681 ctacaaagag cagctggcca agtggcaggg aagactatct gaattggtac gtcatcccgg
    19741 attcaaaatg cactcgttga tcatcgcttt cgagggttcg gatgctgctg gcaagggtgg
    19801 cagtatccgg cgcatcatcg cggcaattga tgcgcgccag tatcagatta ttccggtggc
    19861 cgcaccgacc gaggaggaac gcgcacagcc ttacatgtgg cgtttctggc ggcatatacc
    19921 acgcaccggc cgcgtggcga tttttgaccg gacctggtac ggccgggtgc tggttgagcg
    19981 cgtcgaagga ttctgcgcag aagcggactg gttgcgtgcc tacacggaga tcaatgactt
    20041 tgagcatgag ttgcaaaagg atggtgcgat tgtcatcaag ttctggctgc aaatcagcga
    20101 caaggaacag ctcagacgct tcaaggagcg tgagaatatc gagttcaagc gtttcaagat
    20161 taccgaagaa gactggcgca accgtgaaaa aagcgaggcc taccatactg ccgtttgcga
    20221 tatggttgac cggacaagta ccagtacggc gccgtggacg ctgatcgagg cgaacgataa
    20281 aagtttcgcc cgggtcaaga ttctgcgaac catttgtgag cgtctggaag ccgctttgaa
    20341 catcgagaaa tcaaaagaaa aatccaagga taaataacaa catgcatgat gaactgagtt
    20401 ccgcccactt cgtatcgctg ttcaggtcgg ttgctcccta tgtaaatttg tttcgcggca
    20461 aaacctttgt tattgccttt ggtggcaagg cgatttccgg gccgctggct cgaacattgg
    20521 cctatgacgt gaatctgctt gctgccttgg gcgtgcggtt gttgctggtg catggcgccc
    20581 gcccgcagat tgaggaggag ttgatcgaga agggaatacg gtcggtcacc catttaggct
    20641 atcgcgtcac ggatgcggcg acccttgaat gcgtgattga cgcggtgggc agtgtctacc
    20701 tggaaattga cgcgttgcta tctcagggct tgcccgatac gccgatggcc aacagcagga
    20761 ttcgcgtcat tgccggtaat ttcattacag ccaaaccgct cggcgtgctt gacggtgtgg
    20821 acatgcagta tgccggaagc gttcgaaaga tcgatgccga ggggattcgc gcccaactgg
    20881 gtatcggcaa tatcgtgatt ctttcgtgcc tcggcgccag ccccacgggc gaaatattca
    20941 atctcgccat ggaggaagtg gctgagtccg cagcaaaagc catcgccgca gacaagctta
    21001 tcttcttgac cgatagtcag ggcgtgaccg acagtgacgg aaagctgctc gacgagttga
    21061 ccgccgactc ggcggagcgc ctgatcgcga ttggtgactg gctgtctccg gatatcaagc
    21121 gatatctgcc ctgtgcagta cgcaccagtc gctccggcgt cggtcgtgtt catctgatca
    21181 actacaatga ggacggcacg ctgttacgcg aactcttctc gcgcgacggg gtgggtacta
    21241 tcattacccg cgagtctctc gaaattctcc gcgacgcgcg tccggacgac atcagtggtc
    21301 ttgtcgcgct gatcgaaccc ttggaagaga tcggcgcact cgttcgtcgt tcgcgtgagc
    21361 ttctcgaacg tgaaatcacc cgtttctcgg tggtggaaca cgacgggttg attgtgggtt
    21421 gtgccgcgct atatccatac ggcgacgatc aggctgaact ggcctgcgtg gctgtacatc
    21481 cccactatcg tcgctggggt tatggcaatc aactgatcaa acgcatcgaa acgcgcgcca
    21541 gggctgtggg gatcaagcgc ttgttcgtac tgactacggt gacatcgcac tggttcaagg
    21601 aaagaggctt tgccgagcag tcggtcgatc agctgccggc agagaagcag cttgtctata
    21661 atttgcaacg ccgctccaag gtattcgtca agccggtcta ggccattgcc gtcagcgtgc
    21721 gcagtataat cagggcttct tttagcgatg tgcgttcatc gcattacgga gtcaatctca
    21781 tggcaagaat ggttacctgt atcaaactcg gtcgtgaggc cgaagcgctt gattttccgc
    21841 cttatccggg ggaacttggc aagcggattt acgaggaagt gtccaaggaa gcctggcagc
    21901 agtggatacg tatgcagacg atgatcatca acgagaatcg tctgaacttg gctgatgccg
    21961 cacatcgaaa gtacctggcc gagcagattg agaagcactt tttcggaagt ggtgttgatc
    22021 aggtgcaagg ctacgtcccg ccaaattcct gattcagata ccgtaaccag tcaagcgctt
    22081 tctcggacgc tacgactggt tcaggcagtc ccgctagcaa cgctcaactg ttggcgatct
    22141 ttgcatcgac ttcgtcgact gtcgcgtgac gaacgttcag tcccttgacc atgaatatca
    22201 cgtactccgc gatgctgctt gcgtgatctc cgatccgttc gatagccttg gcgatagaca
    22261 aaatatccag tgaacgtgtg atggttcgcg ggtcttccat catgtaagtg atcagctggc
    22321 gagtgacgga caaatattcc gcattgacca gactgtcgag gtgcatggct ctggtaacaa
    22381 gactgatatc aagacgggca aacccgtcca gtgaaaggcg caacattttc aatgccgtat
    22441 cggccatgtg gttcaactcg atattgagcg aaccagcgga ggggtggtgc gtgagcgcga
    22501 tccccagacg agcgatcttt cgtgccttgt cgccaacgcg ttccagatcg gcaatggcct
    22561 tgagcacaga cacgatcatg cgcaggtcat ttgccgtagg ttgccgcttg gcgatgatct
    22621 gataacacga ggtctcggtc tcgatctcga tctcgttgat cagcgcgtcg ttgcgaatga
    22681 ccgtctcaag cagcggatgg tcacccgtgt tcaggccttc catcgccttg cgaacctgtt
    22741 cctcagccaa tcctcccatt tgcaggacgc gagtacgaag gttttcaagt tcaagatcga
    22801 aattcttgga agtgtggtcg ctcattcagc ttgctccaaa actattttgt atttgatgaa
    22861 cacgggttca agtctaacgc atcatgatta catatttgtt tcaatcggag gtcagcgttt
    22921 gcacgccagt actcgtgccg agtaaacaga cgtcagcagg tcggcgggcg aacaggccgt
    22981 tgctgaccac gccaacgatc tggttgattc gcgtttcgag ttcaaccggg tcaacaatca
    23041 ttaatccttc tacatccagg atcaagttgc cgttgtccgt tacgaaaccc tctcgcagta
    23101 cgggcgaacc tccaagtttt gtcaattcgc gggtgacgta cgcacgggcc atcggaatga
    23161 cttcaacagg cagcgggaac gcaccaagaa ggtccacctt ttttgattca tcgacaatgc
    23221 aaataaacct tgtggcgacc gcagcgacta tcttctcgcg ggtcaatgcg ccacctccac
    23281 ctttgagcat gtgcatcccg ggggtgatct catcggcacc atcaatgtag acagggagtt
    23341 ccgatatgtc attgaggtcg aataccctga tgccatgacc ttccagacgc ttccttgtgt
    23401 cctcggaact ggcaacggcg ccagcaatct gattcttcat tcccgaaagc tcgtcaataa
    23461 acaatctggc ggtcgatccc gtgccgacgc caactatttc ctcctcaacc acgtagtcga
    23521 gagcggcccg ggctgcggcg agtttggcga gcttgagttc atcctggttc atgactaggc
    23581 gtgtcctgca acggtttggt tggaatcatt ctatccattt ttcccagccg tacgcagcga
    23641 tgaaaggcct tgttcgcaga aatatcatca agccagttca ctggaaatgt catcaaaaag
    23701 aaatcgtcgt gtaatgacag ctcagcaaca tccgcgacat gcctaccgtt cgatcaatat
    23761 ttctttccga tatccatctt ggcacacggg cctgccaggc cgatcgtttg ctcgagttcc
    23821 tgcgcagcta tgtggccgag aacgtttttc tgatcggtga catcgtcgat ttctgggcca
    23881 tgcgtcggag catcgtatgg acctccgagc agaataccgt ggtgcagaaa atcctcaagc
    23941 gggcgcggcg cggagaacgt gtcgtcttca tcccggggaa tcacgacgaa gcgctgcgtg
    24001 actatatcgg cattaccttt ggcgacatcg aggttgttcg cgagcacgtt catcagcttg
    24061 ccgacgggcg caggtttctg cttctgcatg gcgacgaatt cgatcagatt acccggcacc
    24121 atcgttgggt tgccgtactg ggcgacgtga tgtacgacgt gctggttcgc ctgaacgcca
    24181 tgatagcttt cgttcgccgc cgatttggaa tgacctgcta ctggtctctc gccggctacg
    24241 caaaaagacg ggtcaaaaag gcgttgcagt tcatattcga cttcgaggat agcgccagcc
    24301 atgcggctcg acaacgcggc ttgagcggaa tcatctgcgg gcacattcac agcgcagtca
    24361 ttcgtgaggt gggtggggtt acgtacgtga actgtggcga ctgggtcgac agttgtacgg
    24421 cgattgtcga acacctcgat ggccgtctcg agcttatcgc ctgggacagt agaacaatcg
    24481 gtcaggttga agttgatgtc acggaggagg aaatggcatg cgcgtcctga tggtctcgga
    24541 tgtctatttc ccgcgcatca atggcgtctc gaccgctata cagacctatc agcaggcact
    24601 tcggtcgcat ggcgtcgagg tgagtctgat cgcgcctgat tacggtcagc cttgttcaga
    24661 gccatggatt accagggtgc cggcctggcg ggtgcccggt gatcccgagg accgcctggt
    24721 acgctggaca gaaatgcacc gagtcgtcgc tgaagccgtc aggcagggct gtgacctgat
    24781 tcatgtacag acgccctttg tcgcgcatta tgcagcgcgc gccgcgtcgc tgaatcatgg
    24841 aataccgatt gttgcgactt accacacctt gttcgaagaa tatctaaagc actatgcgcc
    24901 actcattcca gcgccctggt tgcgtgggct ggcgcgccgt ttttcccgcc gccagtgtaa
    24961 cgtgctggat gcggtcattg taccgtccag ggcaattcac gaacggctgg ccgcgtatgg
    25021 cgtgaagact ccgctgcatg ttctgccgac aggcatttcg ctacggatgc cagatcatga
    25081 caaccgcagt gcattccgta ctcgcttcgg tatcgaagca tcgcggccgg tcgcgttgtt
    25141 tgtcgggcgc gtcgcgcatg aaaaaaacat cggcttcctg cttgatgtga tcgatctggc
    25201 tcgtgattcg attcccggca tactcctggt gatcgccggc gaaggcccgg cattgccgca
    25261 gcttcggcaa acggttgccc atcgcgggct gcagcaaaac gttcaattca ttggttatct
    25321 tgatcggaca accgatctgc cttcctgcta cgcagcggcg gatgtt
//
LOCUS       DAORPM010000004        24707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_325_length_24707_cov_12.737506, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000004 DAORPM010000000
VERSION     DAORPM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 24707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24707
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_325_length_24707_cov_12.737506"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..627)
                     /locus_tag="PLD53_00365"
     CDS             complement(10..627)
                     /locus_tag="PLD53_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRC59385.1"
                     /translation="MPLSVSSPTLGQTNRRLLKKLLALVVLAFAFGFALVPLYDAFCL
                     VTGLNGKTGNAAQAGNFGFGGINKSVTAPPSSIDMNRVVTVEFTGTVMPGLPWEMLPM
                     TSKLDLHPGELHLVKYRVHNRSDKTIVGQAIPSVSPGLAAQHFEKIDCFCFAQQTLAP
                     GETKELPLTFIVKPEIDNDIHTITLAYAFFHVDGEKSAVKNQVSR"
     gene            complement(762..1127)
                     /locus_tag="PLD53_00370"
     CDS             complement(762..1127)
                     /locus_tag="PLD53_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HRC59386.1"
                     /translation="MHDSFMIVLSTLFVVVFAIMMHSMFKHRRNCDHSTAKLTGANGT
                     VQWLWALVPFAILAFVDIALINAPKDRNAHALKEGGLTIAQNQTSALSSLVDSTQTLR
                     SSAADSEESVLVTAYRAHR"
     gene            complement(1230..2066)
                     /locus_tag="PLD53_00375"
     CDS             complement(1230..2066)
                     /locus_tag="PLD53_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRC59387.1"
                     /translation="MQAHSSDSVYYVPPPSIWPLVGSVALLTLATGFVLLINKIAAGP
                     YIMTLGVGILLVMLFGWFGAVIKESLAGKYSQQVDLSFRWGMVWFIFSEVMFFAGFFG
                     ALYYARSFAVPWLADTQLLWPGFSAAWPTAGPGLNTVISPVNAWGIPAINTLILLTSG
                     ATVTWAHWGLQKDNQFQLKLGLALTVLLGVIFIGMQIHEYGEAAFTIRDGIYGATFYM
                     LTGFHGFHVIMGTVMLIVILLRSMAGHFSPSHHFAFEAVAWYWHFVDVVWLLLFIFIY
                     WL"
     gene            complement(2089..3675)
                     /gene="ctaD"
                     /locus_tag="PLD53_00380"
     CDS             complement(2089..3675)
                     /gene="ctaD"
                     /locus_tag="PLD53_00380"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRC59388.1"
                     /translation="MNTTAVNVAADHHDHDHAPSGVMRWLTSTNHKDIGTLYLWFSLF
                     MFFFAGALAMLIRAELFEPGLQLLHPNVFNQLTTMHGLIMIFGAIMPALVGFANWQVP
                     LMIGASDMAFPRLNNWSFWLLPAAGTLLVASFFVPGGAIAGGWTMYPPLYIQGGIAYD
                     MTILAVHILGVSSIMGAINIITTILNMRAPGMTLMKMPLFVWTWLITAYLLVAVMPVL
                     ACAITMLLTDRHFGTHFFDAAGGGDPVMFQHIFWFFGHPEVYIMILPSFGIISTIIPT
                     FARKPLFGYVSMVYAVASIAFLSFIVWAHHMFTTGMPVAGQLFFMYATMLIAIPTGIK
                     VFNWVATLWRGSITFETPMLFSLAFVFLFTIGGFSGLVLGLVPVDIQLQDTYYVVAHF
                     HYVLFSGAAMSIMAGVYYWLPKWTGHMYDEKLGKLHFWLTAIAFNITFFPMHFLGLAG
                     MPRRIPDYSLQFTDFNRIASLGGFLLGVSQLLFLYVVIKCVRGGKPAGNKPWDGADSL
                     EWTQTSPPPYHSFVTPPVIK"
     gene            complement(3689..4900)
                     /gene="coxB"
                     /locus_tag="PLD53_00385"
     CDS             complement(3689..4900)
                     /gene="coxB"
                     /locus_tag="PLD53_00385"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRC59389.1"
                     /translation="MWQNIALVATPKKSKGGEILKVKNFQDRRSGPSFFPVYSLWGLL
                     AGLIPTMAQAEYALNLPAPASDLAQSIYDLHTLILWICLAILIVVFIPMALALVRHRK
                     SVGHVAAKFHDNLLVEIIWTIIPILILIYMAWPAAALVVSMKDTSKEDLTIKVTAHQW
                     KWEYEYLGDNIRFISNSSTPKEQIEGLQPKGEHYLLEVDRPMVVPAGKKVRLVMTASD
                     VIHAWWVPAFGVKQDAIPGFIRDTWFKVDKPGTYRGQCAELCGVNHGFMPIVVEVVPP
                     EQFASWKNEQIALVAAAAANANKVYELAELKDIGEKVYAANCAACHQPNGEGLPGAFP
                     GLSGSKMVNEDKAGHIDIVINGKPATAMAAFGKQLSDLDIAAVVTYERNSWGNKTGDV
                     VQPADIAAHRK"
     gene            complement(5061..6200)
                     /locus_tag="PLD53_00390"
     CDS             complement(5061..6200)
                     /locus_tag="PLD53_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRC59390.1"
                     /translation="MENFFASCPRGLEPLLAEDLAVAGVAESRQIPGGVHFVADWPTC
                     YAANLHSRIATRILWRVARGPYAKEDDIYRQALALPWPTWFSASQTIRVDITAVKCPL
                     RSLEFMTLRIKDAICDRFRADVGKRPSVDTREPEVRLHGFLSANECTLYIDTSGAPLY
                     QRGLRQKTVEAPLKENVAAGILRLSGWQPGIPLLDPMCGSGTFLLEAAQIALNIAPGA
                     GRDFGFQRLRNFRLKEWKHLLDSAMDAEKPAANAQIYGSDISPVAVRAALANLDRAGL
                     LTAVTLSSGDILEIDAPAKAGVLVTNPPYGERLSEQDELAAFYPQLGSALKRKFAGWN
                     CYLLSADMRMPKLMRLMSSKKTPLYNGAMECRLFEFKMVAGSNRR"
     gene            complement(6211..6633)
                     /locus_tag="PLD53_00395"
     CDS             complement(6211..6633)
                     /locus_tag="PLD53_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRC59391.1"
                     /translation="MAYLVVKALHIVLVISWFAGLFYLPRIFVNLAMVPQESTAEYDR
                     LLLMAGKLYRFMTPIGFLSIAFGLWLWLGFGVSGGWLHAKTMLVVVLVGYNLYCRKLL
                     KAFQSGKNKFNHVWFRWFNEMPVLLLLAIVFLVTFKPF"
     gene            complement(6633..7514)
                     /locus_tag="PLD53_00400"
     CDS             complement(6633..7514)
                     /locus_tag="PLD53_00400"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC59392.1"
                     /translation="MQKLLIVGCGDIAFRVLPRLLGHYRVFALLRDPVQFASWRANGA
                     RPIRADLDHASSLQRISGLADIVLHFAPPPERGVVDTRTRRLLAALARGKSLPQRLVY
                     ISTSGVYGDCGGARIDETRPPHPTSARAMRRLDAEHQLRRFGRQNGIRVSILRAPGIY
                     AADRLPTERLQKRVHALRAEDDVFTNHIHADDLAMLSCAALRYGRANRCYNATDDSAM
                     KMGEYFDLVADRFGLPRPPRISRSEAQQQMSALQLSFMSESRRIDNQRIKKELRAKLR
                     FPLVVDGVNAAWQERNR"
     gene            7554..8627
                     /locus_tag="PLD53_00405"
     CDS             7554..8627
                     /locus_tag="PLD53_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HRC59393.1"
                     /translation="MGFLVSVQPGLHSQHEFEAEADETILDAALRQGLLMPYGCRDGI
                     CGTCRGRVLSGIVDHGKAPIDTLSETDRRAGFALFCCAKAQSDLAIESREIRSAQEIP
                     VRTLPARVQKLTLAAPDVMIVELKLPASDRLQFFAGQYIEILLKGGRRRAFSLANAPH
                     DDGCLQLHIRHVPGGQFTDHVFNAMKERDILRINGPHGSFYLREDSAKPMLLVAAGTG
                     FAPIKAIVEHAIAEKTTRPMAIYWGGRERADLYLMLLPEQWARQPGIRFVPVLSDTPQ
                     CEKWCGRIGFVHAAAMQDFPDLSGHQVYVCGSPEMVAAARRDFVGKCNLPENEFFADS
                     FEFANDDVSAITDLAKNPSNISG"
     gene            8633..9808
                     /locus_tag="PLD53_00410"
     CDS             8633..9808
                     /locus_tag="PLD53_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRC59394.1"
                     /translation="MPDVYISRQPLVNRQSKIIATRLTLHFDDGASTKDAAKTLDALT
                     EFWPLGEKLVFISCGNAPCDAELLDWQIPENATIEVDSASFVEPEAAILIDALKAANA
                     SLCLVFDTQAKAALSSGVSFRFIAFDAQRFSPAQLKSFAAQTQPYGIGVAINVRDSQG
                     FQDCLDAGVNAAASWFFKHPTKAPAKALSPGQAQIVRVLNLVRKNADIKEIEAALKQD
                     VALSYKLLRYINSAGFGLSCEVQSFRHAVTILGYNKLNKWLSLLLATASKDPMAPALM
                     HTALTRARLMEVLAQGLVDRQEYDNLFITGAFSMLDALLGVDMDKVLESMSLPEPICD
                     ALLGNGGVYGPFLDLAKSSEGEDGKELAEQVGMLGLSADQFNRAQMQALAFADTMEL"
     gene            complement(9887..10630)
                     /locus_tag="PLD53_00415"
     CDS             complement(9887..10630)
                     /locus_tag="PLD53_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC59395.1"
                     /translation="MTPRSSKPIWEAALLEVTGLRVAYGGIQAVRGITFHINQGEMVA
                     LIGANGAGKTSTLKALSRLLDSAGGSVRFCGKELSALPAHQLVSEGIALVPEGRGVFP
                     RMSVSENLLMGAYVRRDKAEIEVDREQVFSLFPRLMERAHQLAGTLSGGEQQMLAIGR
                     ALMCRPKLLLLDEPSMGLAPLMVQKIFEVIRTVADKGMTVLLVEQNAKLALEVSQRGY
                     VMESGEITLTDESATLLANPKVREAYLGE"
     gene            complement(10584..11354)
                     /locus_tag="PLD53_00420"
     CDS             complement(10584..11354)
                     /locus_tag="PLD53_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC59396.1"
                     /translation="MSVTILEARAIDKHFGGVTALRDVSLTIKQGEIYGLIGPNGAGK
                     TTFFNVLTGLYHPDGGDLVFAGEKLQASAPHKVAKRGIARTFQNIRLFANMTAMENVM
                     VGRHVRTHAGVFGAVLHDRRTINEEAAIRRRSEELLHYVNIADRANDLARNLSYGDQR
                     RLEIARALATDPKLLALDEPAAGMNGAETTDLRVLIEGIRKDGITILLIEHDVKLVMG
                     LCDRVAVLDYGQKICEDIPSVVQNDPKVIEAYLGGSIA"
     gene            complement(11351..12394)
                     /locus_tag="PLD53_00425"
     CDS             complement(11351..12394)
                     /locus_tag="PLD53_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC59397.1"
                     /translation="MGIVLAALPFMIGSGLGNSWLRIIDFAMLYIMLALGLNIVVGFA
                     GLLDLGYIAFYAVGAYLYALLASPHFGLHLPVWLIIPAGAALAGFFGALLGAPTLRLR
                     GDYLAIVTLGFGEIVRIFLNNLNAPINITNGPQGVSGIDPLHFGSITLAKPIDFLGFT
                     IPSLHAYYYLFLALALFIVFVTIRLENSRIGRAWVAIREDEIAAKACGINTRNIKLLA
                     FTMGASFGGVAGGLFAGFQGFVSPESFNLMESIMVLCMVVLGGMGNIPGVILGGVLLT
                     ILPEIFRHGAGPVQLALFDAILLDPEALRMLLFGLALIVVMLYRPAGLWPSTTRRREF
                     KGESTTDVLKEGE"
     gene            complement(12463..13386)
                     /locus_tag="PLD53_00430"
     CDS             complement(12463..13386)
                     /locus_tag="PLD53_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC59398.1"
                     /translation="MDIFIQQIINGLILGSIYALVALGYTMVYGIMGLINFAHGEVVM
                     IGAMVALTVIKLLESSGLPVFIVILFGLAAAATVCMAVGFTIERIAYRPLRHAPRLAP
                     LITAIGVSIVLQNLAMLIWGRNYHAFPPILPTSAFNIFGATITSLQVAIVLVAALMMS
                     GLMILINRTRLGRAMRATSENPPIARLMGVNINQIISFTFIIGSALAAVAGLMVSANY
                     SIAHYNMGFMLGLKAFTAAVLGGIGNVAGAMLGGVLLGLIESLGAGYIGDLTGGFLGS
                     HYQDIFAFFVLIGVLVFRPSGLIGERVSERA"
     gene            complement(13710..14888)
                     /locus_tag="PLD53_00435"
                     /pseudo
     CDS             complement(13710..14888)
                     /locus_tag="PLD53_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
     gene            complement(15081..15392)
                     /locus_tag="PLD53_00440"
     CDS             complement(15081..15392)
                     /locus_tag="PLD53_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRC59399.1"
                     /translation="MKTIYLSLLVAAGLLSAGAAQAADEMALAKSKNCLACHAVDKKL
                     VGPSYQDIAKKYTAKDEAMLVEKVLKGGKGNWGQIPMPPNASVTPDEAKKLVTWILSL
                     K"
     gene            complement(15570..17429)
                     /gene="ilvD"
                     /locus_tag="PLD53_00445"
     CDS             complement(15570..17429)
                     /gene="ilvD"
                     /locus_tag="PLD53_00445"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRC59400.1"
                     /translation="MPDYRSRTSTHGRNMAGARALWRATGMKDGDFGKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGIAKEFNTIAIDDGIAMGHDGMLYSLPSRDLIAD
                     SVEYMVNGHCADAMVCISNCDKITPGMLMAAMRVNIPAIFVSGGPMEAGKVKLGGKTI
                     SIDLVDAMVKAVDSTISDADLEEIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GTLVATHADRKGLFVKAGHQIVELCRRYYEQDDASVLPRSIGSFEAFENAMTLDVTMG
                     GSTNTVLHLLAAAQEAGIDFTMQDIDRISRKVPCLCKVAPMTDKYHIEDVHRAGGIYG
                     ILGELKRSGLLHLGVPTVHSRTLDEAIMHWDVMHHSRDLKVYEYFRASPGGVPTQTAF
                     SQNRRYPELDLDRTRGCIRDKSNAYSKEGGLAVLFGNIALDGCIVKTAGVDESIWTFS
                     GKARVFESQDAAVQAILADQIVAGEVVVIRYEGPKGGPGMQEMLYPTSYLKSKGLGKA
                     CALLTDGRFSGGTSGLSIGHVSPEAAEGGAIGLVEEGDTIEIDIPGRRIHLAVTDAVL
                     QQRRETMDARNEQSWQPASERTRIVSKALQAYALMASSASTGAVRDLSLLKRR"
     gene            17553..18365
                     /gene="lgt"
                     /locus_tag="PLD53_00450"
     CDS             17553..18365
                     /gene="lgt"
                     /locus_tag="PLD53_00450"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRC59401.1"
                     /translation="MLIHPQFDPVALSLGPLSIHWYGLMYLAAFFQFWWLGKRRIQTH
                     PQLSKSGWTVPQLDDLLFYGVLGVILGGRLGQVIFYEPGYYLSHPLEILAVWKGGMSF
                     HGGFLGVLVAMGLFARRTQRTWLDITDFIAPLVPLGLAAGRIGNFINGELWGRISDPA
                     LPWAMVFPKVDDLPRHPSQLYQAGLEGVLLFIVLWYFSRGVRPRGAVSGVFLIGYGSL
                     RFVAEFFRMPDDGIFGLSEVISMGQWLSLPMILIGVVMLVRSLRRGVGEADS"
     gene            18384..19772
                     /locus_tag="PLD53_00455"
     CDS             18384..19772
                     /locus_tag="PLD53_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HRC59402.1"
                     /translation="MTEGIVSRGIQKVRKMLSEHSSTGVLSERQLAQIREQLKECAVG
                     LGGEISARQRAARLADTYLSLNDAGRAAFLHIVATEFGPDPKSVEKAHTRYQAAIGTD
                     SQWAAESALRGELRSIPLRILTQFNALPQGVKFLVDMRADLLRYVDADLALRSLDREL
                     EYQFGAWFDVGFLELQRISWNSPAILLEKLIEYEAVHEIRSWSDLKNRLDSDRRCYAF
                     FHPRMPMEPLIFVEVALVDELADNVQALLDERAPVFDAQRAKTAIFYSISTTQPGLRG
                     VSFGNFLLKRVVDDLKRDFSRLATFATLSPIPSLRRWVEKNPGVWQQAFTEDMVQRVA
                     RHVGPKGPVIDSAVGIKALLVDDAWAANTRLARALQPGLVRMAARYLLHAKAGTRPYD
                     PVARFHLGNGARIERINTLADISTNGLQQSYGLMVNYLYDPDAIETNLEAFSREGVVA
                     TSGTVRRSAQTT"
     gene            19881..20291
                     /gene="mce"
                     /locus_tag="PLD53_00460"
     CDS             19881..20291
                     /gene="mce"
                     /locus_tag="PLD53_00460"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRC59403.1"
                     /translation="MNLSHIEHIGIAVKSLDEAIPFWEKQLGLKCYSIEEVKEQKVRT
                     AFFKIGQSKIELLESTEPDGPIGKFIEKKGEGIQHVAFAVSGIQDNLTELEGKGVQLI
                     DKAPRKGAEGLNIAFLHPKSTHGVLLELCEDPKK"
     gene            20360..21967
                     /locus_tag="PLD53_00465"
     CDS             20360..21967
                     /locus_tag="PLD53_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59404.1"
                     /translation="MAESSVPIGQADNKAAFVDANGATAWLAGQPQANAPAMLAGLVA
                     QIQAFNSAFTAPRERFKTLEALRKTIFAVSQECQRRYENKPLPLLPAEQTALDMSRRL
                     WRSCAVGYMHCLRACLEKDGSIASHGAQVAHRVLACLRMEQMNCYFAGADLDGEFWRN
                     LHSVWVSAEQLGKTSDLVEDRLLGETSKSTVRGQYCMVLTLHLARPFTLSRGQLAAAV
                     RWFARWRELAAVLAEPNQNPKSCCIAINLSQDRPIHESAQGASVGRWLSLDGVLQKMR
                     KRLELLAKGDSPEVLKLGSGLSSEACVALLTTLSEHLRYPQKTPIGALLEASPITVAA
                     GLENINRLLGGKGLKISVQHASLGTQLSHHEQVALFGRAVRDAEESGECKSEIWQITQ
                     QAPGVLNLFCPAGNSEARLVLGGLLCVKLPQHEHFTLATISGLNTRADGGLCVAASLF
                     DGEPSPLIAEVREKPAGKLSRHPAFLLTGREEGSTPLVVLPIGLPARALSFRFFEARE
                     QSLIALRLVENLGRGGDIERWSAAIDA"
     gene            22008..22343
                     /gene="cutA"
                     /locus_tag="PLD53_00470"
     CDS             22008..22343
                     /gene="cutA"
                     /locus_tag="PLD53_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRC59405.1"
                     /translation="MNTLLVLTNLPDRATAETLAARLVEDRLAACVNILPPCHSIYRW
                     QGEVETADEVPLLIKTTEARYPALEEAIRARHPYETPEIIALPVLLGLPGYLAWVAAE
                     TQSDDWSAP"
     gene            22340..24097
                     /gene="dsbD"
                     /locus_tag="PLD53_00475"
     CDS             22340..24097
                     /gene="dsbD"
                     /locus_tag="PLD53_00475"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HRC59406.1"
                     /translation="MMRQLLRCLMISLLLCSLANAEDLLHPLAAFKPTARALDGQTIE
                     VRFEIAPAYYLYRDKFRFSAEPPGVQLGTPILPKGKEKNDETFGKVVVYYDEVLIRLP
                     VERNSSGKLPLTLNITSQGCADAGVCYPPQKQALSLELPDPSAVPLDTPSGDESGRIA
                     QLLKNAGFWLIVLSFFGFGLLLSLTPCVFPMIPILSSIIVGAGRDGHGVSHARGFALS
                     LAYVTGMAITYAAAGIAAGLTGTLISTALQNSWVLGGFALIFVVLSFSMFGFYELQFP
                     TFLQSKVSEEASHIKGGSLPGVAAMGVLSAIIVGPCVAAPLAGALLYIGQTGDALLGG
                     LALFFMAFGMGTPLLAVGLSAGTLLPKSGPWMEAVKKAFGVILLASAVWTVSPVIPVV
                     VQMLAWALLLIIPAIYLHALDPLPAHAKGWQRFWKGIGMVMLITGAAMLVGALAGAKD
                     PLQPLSGLRGGAQSSEISRLPFERIHSIAELDARIQSSAKPVMLDFYADWCVSCKELE
                     RFTFSDIRVQQKLAGWTLLQADVTANTEEDKALLARFKLFGPPGIIFFDASGKEDMGV
                     RVIGFQGADEFLATLSRLR"
     gene            complement(24109..24387)
                     /locus_tag="PLD53_00480"
     CDS             complement(24109..24387)
                     /locus_tag="PLD53_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02647 family protein"
                     /protein_id="HRC59407.1"
                     /translation="MPYTSDLVHELNTLIRFDLETGLQGIKVHKTADKQVIAAVRRLH
                     AKGLVTQADGGYLTSLGREAAEFAQTVLGILTAGVAASISAHEPIPEG"
     gene            complement(24439..>24707)
                     /locus_tag="PLD53_00485"
     CDS             complement(24439..>24707)
                     /locus_tag="PLD53_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59408.1"
                     /translation="ASRVRSDDIREESRIALMNAVNPRYVLRNCLAQQAIDGAENGDP
                     EMIHQLLDVLRNPYDERPGCEAFAARRPDWARHKAGCSMLSCSS"
BASE COUNT         5330 a         6878 c         7150 g         5349 t
ORIGIN      
        1 acgccggagt cagcgtgaga cttggttttt caccgccgat ttctcaccgt cgacatgaaa
       61 gaatgcatag gcaagggtga tcgtatggat gtcattatcg atttccggtt tgacgatgaa
      121 agtcaatggt aattccttcg tctcgcccgg agcgagggtt tgttgcgcaa agcagaagca
      181 atcgattttc tcaaaatgtt gtgcggccaa acccggagaa acgctcggta tggcttgccc
      241 aacaatggtt ttgtccgaac ggttgtgtac gcggtacttg accagatgca gttcacccgg
      301 atgcagatcc agctttgaag tcattggcag catttcccat ggcaggccgg gcattacggt
      361 gcctgtaaat tcgaccgtca cgacgcggtt catgtcgatg ctgctgggtg gtgccgtgac
      421 tgacttgttg atacctccaa aaccgaaatt gccggcttgt gccgcattcc ctgtctttcc
      481 attaagtcca gtgaccaggc aaaacgcatc atatagcggc actaacgcga atccgaaagc
      541 gaacgccaga acgacgagtg caagcagctt cttgagcaga cggcggttgg tttgtccaag
      601 tgttggacta ctgacagaca ggggcatttg actgcatcag cttttcaaaa ataacacgag
      661 gaagaaaacg gcgcacgcaa cggcagcgat agtccagccc aaacgtcgat tgtccgcatt
      721 tcgctggttg cccattgccg aatctgaggt cttcgttgtt gtcatctgtg agccctgtaa
      781 gcggtcacta aaacagattc ctcactgtcc gctgctgaac tcctcaacgt ctgcgtgctg
      841 tcgaccagcg aagacaaggc tgacgtctgg ttttgcgcaa tggttagacc gccttccttc
      901 agggcatgtg cattgcggtc tttcggagcg ttgatcagcg cgatgtcaac gaaggcaagg
      961 atggcaaatg gaacgagtgc ccacagccac tgcaccgtcc cgttcgcccc agtgagcttc
     1021 gcagtcgagt ggtcgcagtt tctgcggtgt ttgaacatcg aatgcatcat gatcgcaaat
     1081 accaccacaa agagggtgct aagaacgatc ataaagctgt catgcatccg caatcggttt
     1141 ctccagtgat ccaataaaac cgtcgaacaa gggaagctgc atcggcaatc gggaagaagt
     1201 aaggtattgg ccggagagtc cggaagagcc tataaccaat agataaaaat gaacagcaac
     1261 aaccagacca catcaacgaa gtgccagtac catgctacag cctcgaatgc gaaatggtgg
     1321 cttggtgaaa aatgtccggc catagagcgc aacaagatga cgataagcat gactgttccc
     1381 ataatgacat ggaagccgtg aaaacccgtc agcatgtaaa aggtcgcgcc ataaatacca
     1441 tcacggatgg tgaaagccgc ttcgccatac tcatgaatct gcatgccgat aaagatgaca
     1501 cccaagagaa ctgtcagtgc caggccgagc tttagctgaa attgattgtc cttctgcagg
     1561 ccccaatgtg cccaagttac ggtagctcct gaagtcagaa gaatcagggt gttgattgca
     1621 ggaattcccc aagcgttaac cggcgagata actgtgttca agccaggacc ggccgttggc
     1681 caggccgcac tgaatccagg ccacaacaac tgcgtgtcgg cgagccatgg cacagcgaaa
     1741 cttcgggcgt agtacagggc accgaagaac cctgcaaaga acatgacctc agaaaaaata
     1801 aaccacacca tcccccagcg gaatgagagg tcaacctgtt gggagtactt gccggccaga
     1861 ctttccttga taactgcgcc aaaccagccg aatagcatga ccagcagaat tccaacccct
     1921 agtgtcatga tataggggcc agcagcgatc ttgttgatta ggaggacgaa gccggtagct
     1981 aaggtgagaa gagcaaccga gccgactaaa ggccagatcg aaggtggagg gacgtaatag
     2041 acgctgtcgc tggaatgtgc ttgcatggct tctttccctt cgtatggcct atttgattac
     2101 cgggggagtg acaaacgagt gatacggtgg cggcgatgtc tgagtccatt ccagcgagtc
     2161 agcgccatcc cacggcttgt tcccagcggg tttgccgcct cgtacgcact tgatcaccac
     2221 ataaaggaac aacagttgac tgacgccgag gagaaatccg ccaaggctgg cgatcctgtt
     2281 gaaatcggtg aattgaagcg aatagtcggg aatgcgccgt ggcatcccgg ccaggccaag
     2341 aaagtgcatt ggaaagaagg tgatgttgaa ggcaatggcc gtcagccaga agtgcagttt
     2401 gccgagcttc tcgtcataca tgtgcccagt ccattttggc agccagtagt acacgccagc
     2461 cataatgctc attgcggctc cggagaaaag cacgtagtga aagtgggcaa ccacataata
     2521 ggtgtcctgc aactgaatat ccacaggaac cagccccagc accaacccgc tgaagccgcc
     2581 gatggtaaac aaaaagacaa aggccagcga gaacagcatt ggcgtttcaa aggtgattga
     2641 accccgccac aaggttgcta cccagttgaa gaccttgata ccggtaggaa tcgcaatcag
     2701 catggtcgcg tacatgaaga agagctgtcc ggcaaccggc atgccggtgg tgaacatgtg
     2761 gtgcgcccag acaatgaagg agagaaacgc gatcgaggca accgcataca ccatagagac
     2821 gtagccaaac aatggcttgc gtgcaaaggt cgggatgatc gtcgagataa tgccgaatga
     2881 cggcagaatc atgatgtaca cctcagggtg cccgaagaac cagaaaatgt gctggaacat
     2941 gaccggatcg ccaccgccgg ccgcgtcaaa gaaatgagtg ccgaaatggc ggtcggtgag
     3001 caacatggtg attgcgcagg ccagcaccgg catgacagct accagcagat aggcagtaat
     3061 cagccaagtc cagacaaaca aaggcatttt catcaaggtc atgccgggtg cacgcatgtt
     3121 gagaatggtg gtgatgatat tgatggcccc catgatcgac gaaacgccaa ggatgtgaac
     3181 agcaagtatt gtcatgtcat aggctatacc gccctgaata tagagggggg ggtacattgt
     3241 ccagccaccg gcgatggcac cgccaggcac aaaaaacgaa gcgacgagca gtgtgcctgc
     3301 ggcgggcaat agccagaaac tccaattgtt caaacggggg aaggccatat cggatgcgcc
     3361 gatcatcagc ggcacctgcc agttggcgaa accaaccagt gcgggcatga tggctccgaa
     3421 aatcatgatc aggccgtgca tcgtcgtgag ctggttgaag acattggggt gaagcaattg
     3481 cagtccaggc tcgaacagct cggcacgtat cagcattgcc aaggcgccag cgaagaaaaa
     3541 catgaacaaa ctgaaccaca ggtacagcgt gccaatgtcc ttgtggttgg tactggtcaa
     3601 ccagcgcatg acgccagaag gagcatggtc gtgatcgtga tggtctgcgg cgacgttgac
     3661 tgcggttgta ttcattcctg actccttctt acttgcgatg tgcagcaata tcagccggct
     3721 gcaccacatc gccagtcttg ttgccccagc tgttgcgttc gtatgtgacg actgcagcga
     3781 tatcaagatc ggacagttgc ttgccaaagg ccgccatggc cgttgccggc ttaccgttga
     3841 tgacaatatc tatatggcct gccttgtctt cattaaccat cttcgatcca ctgagtccgg
     3901 ggaaagcgcc tggaagaccc tcgccgttgg gctggtgaca ggccgcgcaa ttggcagcgt
     3961 agaccttctc gccgatatct ttgagttcgg ctaactcgta taccttgttg gcgtttgccg
     4021 ctgccgccgc caccaaggcg atctgctcgt ttttccagct tgcgaattgt tccggaggaa
     4081 cgacttcaac aacgataggc atgaagccat gattgacacc acacagctcg gcgcattggc
     4141 cccggtaggt gcctggtttg tcgaccttga accaggtatc gcgaatgaac cccggtatgg
     4201 cgtcctgctt gacgccaaag gcgggaaccc accaagcgtg gataacatca ctggcggtca
     4261 tcaccaaacg aactttcttg cctgcaggga cgaccatagg ccggtcaacc tcaagcagat
     4321 aatgctcgcc cttgggttgc aatccctcta tctgttcttt cggagttgaa gagttgctaa
     4381 taaatcgaat gttgtcgccc agatactcat actcccactt ccattggtga gcggtaactt
     4441 tgatcgtgag atcttccttg ctcgtgtcct tcatcgatac taccaaggct gccgccggcc
     4501 atgccatata gatgagaatc aggatgggta tgatggtcca gataatttcc acaagaagat
     4561 tgtcgtggaa ttttgctgcc acgtgcccta ccgatttgcg gtgccgcact aacgccaagg
     4621 ccatcggaat gaacacaacg atcaggatcg caaggcatat ccagagaatc agggtgtgca
     4681 ggtcgtatat ggattgagcg aggtccgaag ccggcgcggg cagattcaaa gcatattctg
     4741 cttgggccat tgtggggatc aaccccgcaa gcaggcccca cagcgaataa acagggaaga
     4801 atgaaggtcc ggaacgccgg tcctggaaat ttttcacttt caagatttct cctcccttac
     4861 tcttttttgg ggttgcaaca agtgcaatat tttgccacat ttacccagaa atttcattga
     4921 cgtccatata atttttgaaa atttattcat tctggctcgt cttctattcc ctctgaatag
     4981 cactgttcgt cgtactaccc tcttgggggt agcggcacat caagaagtga aattgtcgcg
     5041 caatcgccgt tttaaggcca tcaacgccga ttgcttcccg cgaccatttt gaattcgaac
     5101 agtcggcatt ccatggcgcc gttgtagagt ggcgttttct tgctcgacat caggcgcatc
     5161 agtttgggca tgcgcatgtc ggctgacagc aggtaacagt tccaaccggc aaatttgcgc
     5221 ttgagcgcgc ttccaagctg cgggtaaaaa gcagctagct catcctgctc ggacaggcgt
     5281 tcgccatagg gtggattggt gaccagtacg cccgccttgg cgggagcatc gatttccaat
     5341 atgtcgcccg aactcagcgt aaccgcggtg agcaggcctg cgcggtcaag gttggccagc
     5401 gcggcacgca cggcgactgg cgatatgtcg cttccgtata tctgtgcatt ggcggcaggc
     5461 ttctcggcat ccatggcgct gtcgagcaga tgtttccact ctttgagtct gaagttcctc
     5521 aaacgctgga atccgaagtc gcgcccggcg cccggcgcaa tgttgagtgc tatctgcgct
     5581 gcttctagga gaaaagtacc gctaccgcac attggatcga gtaggggaat accgggctgc
     5641 caaccggaaa gacgaagaat gccggccgct acattctctt tcagcggcgc ctcgacggtc
     5701 ttctgacgca gtccccgctg gtagagcggt gcgcccgacg tgtcgatgta cagtgtacat
     5761 tcgttggcgc tcaagaagcc gtggagacgc acttccggtt cgcgcgtatc cacgctgggg
     5821 cgctttccga catctgcccg gaaacggtca caaatggcat ccttgatgcg cagtgtcatg
     5881 aattcaaggc tcctgagcgg acatttgacc gccgtgatat cgacgcgaat cgtctggctg
     5941 gcactgaacc atgtcggcca cggcaaggca agcgcctgtc ggtagatgtc gtcttccttt
     6001 gcgtacggcc ctcgggcaac ccgccacaga atgcgggtgg cgatacgtga atgcaggttg
     6061 gccgcatagc aggttggcca atcggcgaca aaatgcactc cgccgggaat ctgccttgat
     6121 tcggcaacac ccgccacggc aaggtcttcc gcaagcaaag gttccaggcc gcgagggcaa
     6181 ctggcaaaga agttttccaa aaaacgtatc ctaaaaaggt ttgaatgtaa ccaggaaaac
     6241 aatggccagc agcagcagca cgggcatctc attgaaccag cgaaaccaga cgtggttgaa
     6301 cttgttcttt ccggactgaa atgctttcaa cagttttcga cagtacaggt tatagccaac
     6361 gagtacgacc accagcattg tcttggcatg cagccagccg ccggaaacgc caaagccaag
     6421 ccacagccaa aggccgaaag caatggacag aaagccgatc ggggtcatga aacggtacag
     6481 ttttccggcc atcaacaata acctgtcgta ctcggcggtg ctctcctgcg ggaccatcgc
     6541 cagattgacg aagatgcgcg gcaagtaaaa caggccggca aaccagctga ttaccagcac
     6601 aatatgcagt gccttaacga caagataagc catcaccgat tcctttcctg ccaggccgca
     6661 ttcaccccat ccacaaccag cgggaagcgc aattttgccc ggagttcctt tttgatccgt
     6721 tggttgtcga tccggcgcga ttcactcatg aacgacaatt gaagcgccga catttgctgc
     6781 tgtgcctcgc tgcgcgaaat gcgtggtggt cggggcaggc cgaagcggtc ggcgacgaga
     6841 tcgaaatatt cgcccatttt catcgcggag tcgtcagtcg cgttatagca gcgattggcg
     6901 cgtccgtaac gtagcgccgc acagctcagc atggccaggt cgtcggcatg gatgtggttg
     6961 gtgaacacgt cgtcttcggc ccgtagcgca tggactcgct tttgcaagcg ctcagttggc
     7021 aagcgatcgg cagcgtagat gccgggcgca cgcaggatgc tgacgcgaat accgttttgg
     7081 cgtccaaagc gacgcaactg gtgttcggca tcaaggcggc gcatggcgcg ggcactggtt
     7141 gggtgggggg gccgagtttc atcaatacgc gctccgccgc agtcgccgta tacaccgctg
     7201 gtgctgatgt acacgagccg ctgtggtaga ctttttcccc gtgccagcgc tgcgagcagg
     7261 cgacgtgtgc gggtatccac tacgccacgt tcgggagggg gcgcaaaatg cagcacgatg
     7321 tcggcgagtc cgctgatgcg ttgcagactt gatgcatgat ccagatcggc acggatggga
     7381 cgggctccgt tggcacgcca ggaagcgaac tgcacggggt cgcgcagcag ggcgaatacc
     7441 cgataatgcc cgagcaagcg gggcaggact cgaaaagcaa tatcaccaca gccgacgatc
     7501 agaagttttt gcacggcgca attttaccgg attcatattt tcagggagat tagatggggt
     7561 tcctggtcag cgttcagccg ggtttgcact ctcaacacga atttgaggcc gaggctgacg
     7621 agaccatcct cgatgcggca ttgcggcagg ggcttctgat gccctatggc tgtcgcgacg
     7681 gtatttgtgg cacctgccgg gggagagtgt tgagcggaat tgtcgatcat ggcaaggcgc
     7741 cgatagacac cctgagtgaa actgaccgca gggccggatt tgccttgttt tgttgcgcca
     7801 aggcgcaaag cgatttggcc atcgagagca gagaaattcg ttccgcgcag gaaattccgg
     7861 tgagaaccct gccggcgcga gtgcaaaagc ttacgcttgc agcacccgac gtgatgatcg
     7921 ttgaactcaa actcccggct agcgaccgac ttcagttttt tgccgggcag tacatcgaga
     7981 tattgctgaa gggaggccgc cggcgagctt tttcgctggc caatgcgccg cacgacgacg
     8041 gttgcctgca actgcatatc cggcatgtgc cgggcggcca gtttaccgat catgtgttca
     8101 acgccatgaa ggagcgcgac attctgcgta tcaacgggcc tcatggcagt ttctacctgc
     8161 gtgaggattc cgcaaagccg atgctgctgg tcgctgcggg cactggtttt gcaccgatca
     8221 aggccatcgt cgaacatgcc attgccgaaa aaacgacgcg accgatggct atctactggg
     8281 gcgggcggga acgcgccgat ctctacctga tgctcttgcc cgaacagtgg gcgcgacagc
     8341 ctggtatccg attcgttccg gtgttgtccg atacgcctca gtgcgagaaa tggtgcgggc
     8401 gaatcggttt tgtccatgcg gcggccatgc aggattttcc tgatctttcc ggccatcagg
     8461 tgtatgtttg tggttcgccg gaaatggtag ctgcagcccg acgcgatttt gtcggaaaat
     8521 gcaatctgcc ggagaacgag ttttttgccg attcattcga atttgccaat gacgacgtgt
     8581 ccgctataac agatctggcc aaaaaccctt ccaatatttc aggttgattg ccatgcccga
     8641 tgtctatatt tcgcgacaac ctctggtcaa ccgccagagc aagatcattg ccacccgcct
     8701 gacgcttcat tttgacgacg gtgcatcgac gaaagacgca gccaagaccc tggatgccct
     8761 gacggaattc tggcccctgg gtgaaaaact tgtcttcatc agctgtggca atgcgccttg
     8821 tgatgcggaa ttgctcgact ggcagatccc tgaaaacgca accatcgaag tcgattcggc
     8881 ttcttttgta gagcctgagg cggctatcct gattgacgcc ctgaaagcgg cgaacgcctc
     8941 gctgtgtctg gtttttgaca cgcaggcgaa agccgcactt tcgtccggtg tctccttccg
     9001 tttcatcgcg ttcgatgctc agcggttctc acctgcccaa ctcaagagct tcgcagcgca
     9061 gacgcagccc tacggcatcg gtgtggcgat caacgtgcgc gacagtcagg ggtttcagga
     9121 ctgtctggac gcaggggtga atgctgccgc cagttggttt ttcaaacacc cgacaaaagc
     9181 cccggcgaag gcgctgagcc cagggcaggc gcaaatcgtc cgcgtgctga atctggtgcg
     9241 caagaatgcc gatatcaagg aaatcgaagc ggcgttgaaa caggacgtgg cgctttccta
     9301 caagcttttg cgttacatca actcagccgg ctttggtctt tcctgcgaag tccagtcatt
     9361 ccgccacgca gtgaccatac ttgggtacaa caagctgaac aaatggctgt cgctcctgct
     9421 ggccacggcg agcaaggacc cgatggcgcc cgcgctgatg cacacagcgt tgacgcgcgc
     9481 acggctgatg gaagtgctgg cgcaaggtct tgtcgaccgc caggagtatg acaacctgtt
     9541 catcaccggt gccttctcca tgctcgacgc tttgctgggg gtggatatgg ataaagtgct
     9601 cgagtccatg agtctgccgg aacccatctg tgacgctctg ctgggtaatg gtggcgtgta
     9661 cggccccttc ctcgacctgg caaaatcaag tgagggcgag gatggaaaag aactcgccga
     9721 acaggtggga atgctcggcc tgtccgccga ccagttcaac cgggcccaga tgcaagccct
     9781 ggcctttgcc gacacgatgg agttgtaggg agccttgaac cgggaaggcc agaaacgcga
     9841 atcctttgac gcgctagttg agtcgggtct tcagcggttt gccgccctac tcgccgagat
     9901 aggcttctcg caccttgggg ttggcgagca gtgtggctga ctcgtcggta agcgtaatct
     9961 cgccactttc catgacgtaa ccgcgctggc tgacttcaag cgccagtttg gcgttctgtt
    10021 ctacaagcag caccgtcatg cccttatcgg caaccgtgcg aatcacctcg aaaatttttt
    10081 gtaccatgag tggtgcgagt cccatcgatg gttcgtccag cagcagcagt tttggccggc
    10141 acatcagggc gcgtccgatt gcgagcatct gctgttcgcc accggacaat gttccggcca
    10201 actggtgtgc gcgctccata aggcgtggga aaagagaaaa cacctgttcg cggtccactt
    10261 cgatttcggc tttatcccgg cgaacgtaag cgcccatcag caagttttcg ctgacgctca
    10321 tgcgcgggaa caccccgcgc ccttcgggta ccagtgcgat gccttcggaa accagttgat
    10381 gcgctggcaa ggcggatagc tccttgccgc aaaaacgaac gctaccaccc gctgaatcga
    10441 gcagacggga cagggccttg agcgtactgg tcttgcctgc accattggcg ccgatgaggg
    10501 cgaccatctc accctggttg atgtggaagg tgatgccgcg caccgcctga atgcctccgt
    10561 aggcgacgcg cagaccggtg acttcaagca atgctgcctc ccagataggc ttcgatgacc
    10621 ttggggtcat tttgaacgac ggacggaatg tcttcgcaga ttttttgccc gtaatcgagt
    10681 acggcgactc ggtcgcacag acccatgacc agcttcacat cgtgctcgat gagcaggatg
    10741 gtaatgccgt ccttgcgaat gccctcgatg agcacgcgca aatcggtggt ttcggcaccg
    10801 ttcattccgg ccgccggttc gtcgagcgcc agcaacttag ggtccgtcgc cagtgcgcgg
    10861 gcgatttcga gccggcgctg gtcaccgtaa gacaggtttc gcgccagatc attggcacgg
    10921 tcggcgatat tcacataatg cagcagttcc tcggaacggc gtctgatggc ggcctcttcg
    10981 ttgattgtgc ggcggtcatg caggacggca ccaaacacgc cggcatgtgt cctgacatga
    11041 cgtccgacca tgacgttctc catcgcagtc atgttggcga acaatcggat attctggaaa
    11101 gtgcgcgcga tgccgcgctt ggccaccttg tgcggagcgc tggcctgaag tttttcgccg
    11161 gcgaacacga gatcaccgcc atcgggatga tataagcccg tgaggacgtt gaaaaaagtg
    11221 gtcttgccgg caccgttcgg tccgatcaga ccatagatct caccctgctt aatggtcagc
    11281 gagacatcgc gcaaggccgt tacgccacca aaatgcttgt cgatggcacg cgcctcgagg
    11341 atggtgacgc tcattctcct tccttcagca catcggttgt cgattcaccc ttgaactcgc
    11401 gccgacgggt ggttgacggc cacaagccgg ccgggcggta aagcatgacg acaatcagcg
    11461 caagaccgaa aagcagcatg cgcagtgctt ccggatcaag caggatggcg tcgaagagcg
    11521 caagttgaac cgggccggcg ccgtggcgga atatttccgg aaggatggtc agcaatacgc
    11581 cgccgaggat gacgccaggg atgttgccca tgccgccgag caccaccatg cacagcacca
    11641 tgatcgactc catcaaatta aaggattcgg ggctgacaaa tccctggaaa ccggcaaaca
    11701 gcccgccggc gaccccccca aagctggcgc ccatggtgaa ggccagcagt ttgatattgc
    11761 gggtgtttat gccgcaggcc ttggctgcga tctcgtcttc acggatggcg acccaggcac
    11821 ggccgatgcg cgaattctcc agccggatgg tgacaaacac gatgaacagg gcaagggcga
    11881 gaaaaagata atagtaggcg tgcagtgagg gaatcgtgaa gccaagaaag tctatgggct
    11941 tggccagcgt gatgctcccg aagtgcagcg gatcgatgcc ggatacgccc tgaggtccgt
    12001 tggtgatgtt gatgggggcg ttgaggttgt tcaggaaaat ccgcacgatc tcgccaaagc
    12061 cgagtgtaac gatagccaga tagtcgccgc gcaagcgcag ggttggcgca cccagcaagg
    12121 cgccgaaaaa cccggcgagt gcggcaccgg ccgggatgat cagccaaacg ggcaaatgca
    12181 gtccgaaatg cgggctggcg agtagcgcgt acagataggc gccgactgcg tagaaggcga
    12241 tgtagccgag atcgagcaga ccggcaaaac cgaccacaat gttcagaccg agcgccagca
    12301 tgatgtagag cattgcgaaa tcaatgatgc gcagccatga gttgccaaga ccgctgccaa
    12361 tcatgaaagg caaggcggcg agcacgatgc ccatgacgac gcttgcaata atggcggcgc
    12421 gtttgctgtg ggcgacgttt tcgagtgcgg gtagcaaggt catcatgcgc gctcggatac
    12481 tctttcgcca atcagtccgg agggacggaa aaccagtacg ccgatcagca cgaagaacgc
    12541 gaagatgtcc tgatagtggc taccgagaaa gccaccggtc aggtcaccga tgtagccggc
    12601 gccaagtgac tcgatgaggc cgagcagaac gcccccgagc atggcgccgg cgacgttgcc
    12661 gatgccgccc agcacagctg cggtaaacgc tttcagcccc agcatgaagc ccatgttgta
    12721 gtgggcaatc gagtagttgg cgctgaccat caacccggct acagccgcaa gcgccgagcc
    12781 gatgatgaag gtaaatgaga tgatctggtt gatgttgacg cccatcaacc gggcaatcgg
    12841 cgggttctca gaggttgcac gcattgcgcg cccgagacgt gtgcggttga tcagaatcat
    12901 caggccggac atcatcagag cggcgaccag cacgatggct acctgcaggc tggtgattgt
    12961 cgcgccgaag atgttaaacg cagacgttgg caggatcggc gggaaagcgt gatagttgcg
    13021 accccagatc agcatggcga gattttgcag gacgatggaa acgccaatgg cggtgatcaa
    13081 cggggcgagc cttggcgcgt gccgcaatgg ccggtaggca atacgttcga tcgtaaaacc
    13141 gacggccatg cataccgtcg ccgctgcggc aagcccgaac aggatgacaa tgaacacggg
    13201 caggccactg ctctcaagca gcttgatcac cgtcagggcg accattgcgc cgatcattac
    13261 gacttcaccg tgggcaaaat tgatcaggcc catgatgccg taaaccatcg tgtagcccag
    13321 cgcaaccaac gcatagatgc tgcccagaat caggccgttg atgatttgct ggatgaatat
    13381 gtccattgtc gggaatgcct gaaaatgtgc ctgggtatga cgcgatgcgg tactaaaccg
    13441 aacaaacaaa cggcaccctc aggtgccgtt tgcattgtta cgcggcttgc ctacttcttc
    13501 tctactgcgg ccttggtcgc gtctgctgct tccttcacgt ccttcgcaac ttcggtagca
    13561 accttggcgc cttccgtggc cgcgtccttc atggcgtcct tggcggctgc atcggtcgca
    13621 acagcagcat ccttcaccgc ggccgcggca tccattaccg cttccttggc catagccatg
    13681 ggcgagccac cgatggtttc acggtactcc cactgtccat ccttgacctg atacatgctg
    13741 atggaaccgc cctttatatc gcccttgtcg tcaaactgca ccttggcgct aacgccctgg
    13801 tagtcggtct tgccgatttc gggaaggaat ttggccgatt caaccgaatt cgcgcgcttc
    13861 atggcgtcgg ccatgaccat cacagcatcg tagacgtagg gtgcatagag ctggatttgg
    13921 ccatacttgg ccgtaaaagc ttcaccgaaa gccttgccgc ccggcatctg gtcgagcgga
    13981 acacctggca aggagcagta ctgaccctca ctggcggcac cggcgagctt gatgaattcc
    14041 ggtgtgcagc caccgtcacc cgtaaggaac ttcgctttta ctcccagcga cttcatctgt
    14101 ttggccattg gaccgccttg tgcgtccatt ccgccataga agatcacatc cggcttcttt
    14161 gacttgatct tggtcaggat ggccgcaaaa tcggtagcct tgtcgttggt gaattcgcgt
    14221 gcagcaatct tgccaccaga agcaataacc gctttttcaa actcgtcggc tagaccctgg
    14281 ccgtaggcgg tacggtcgtc gatgatggcg atcttcttgc cacccatttg atgcgcggca
    14341 aattcgccaa gaaccttacc ttgttgcacg tcattggcca tgacgcggaa agccgtggca
    14401 aatccctgct gtgtgtactt tggattggtc gccgaaccgg aaatttgcgg gatgccggca
    14461 tcgaaatata tcttggaagc cgggatggtc gtccctgaat taaggtggcc gatcactccg
    14521 ttaaccttgg cgtcaaccag cttctgggcg acgatggtgc cctgcttcgg atcggcctgg
    14581 tcgtcctctg cgacaagatc gaacttgatc ttggcgccac cgatttcgag gccttgagca
    14641 ttcagctcgt caattgccat acgggcgccg ttctcgttgt ctttacccag atgggcttgc
    14701 ggacctgtca gcggcgcgac gtggccaagt ttaaccgtca gctctggcgt cggtggcggt
    14761 accggagcgg caaccggagc gggtgcagga gcggcgactg gcttgggttc ttccttctgc
    14821 ccgcagccga tcattgcaat agcggccgcg atggcaagtg tcatgctggg aatgcgcggg
    14881 ttcgacatgt atattcctcc ggaagcggta tgaatgaaaa aagtggacac agggggcgat
    14941 tctgctgacc tatcaccttg atgtcaatgt gtagttgagg cggcttgcct cggggtgacg
    15001 cagcacctta acccccaaac aacaagaccg gtgggaaccg gtcttgttgg tctatgttgg
    15061 agtgtaactc ctgagaaagc ttacttcagg ctcaaaatcc aggtaaccag cttcttcgcc
    15121 tcgtcgggtg tgaccgacgc gtttggcggc atcgggatct gaccccagtt acccttgccg
    15181 cccttcagaa ccttctcgac aagcattgct tcgtccttgg ccgtgtattt cttggcgata
    15241 tcctgatacg acggtccgac tagcttcttg tcaaccgcat ggcatgccaa gcagttcttg
    15301 gacttggcca aggccatttc gtccgctgct tgtgctgcac cggcggacag gaggccggcc
    15361 gcaaccagca gggataggta aattgtcttc attctctctc tccaatgatg aaaggtgatt
    15421 gccacggggt gatcatattc caacttgcta ccaaatctca agggcgacat gagattgcaa
    15481 tggggcaaac agactaacgc gtggacccag gataaagttg accgcgattt gaccacgacc
    15541 cgggtggaca ggcttttcct gatgcgaagt caacgacgct tgagcaggct gaggtcgcgc
    15601 accgcaccgg tcgacgccga agaggccatc agcgcatagg cctgcaaggc cttggaaacg
    15661 atgcgcgttc gctcggatgc cggctgccaa ctttgctcgt tcctggcatc cattgtttca
    15721 cggcgttgct gcaatacggc gtcggtcacc gccagatgaa tgcgccggcc gggaatgtcg
    15781 atttcaatgg tgtcgccttc ttcgacaagc ccgatggctc cgccctcggc ggcctccggt
    15841 gagacgtgac cgatggataa gcctgaggtg ccgcccgaga agcgtccgtc ggtgagtagt
    15901 gcgcaggcct tgcccaggcc cttggacttg agatatgagg tcggataaag catctcctgc
    15961 atgccggggc cgcctttcgg gccttcatat cgaattacca cgacctcgcc ggcaacgatc
    16021 tggtcggcga gaatggcttg caccgcggca tcctggcttt caaaaacccg cgccttgccg
    16081 ctgaatgtcc agatgctttc atcaacaccg gctgttttta cgatacaacc atccagcgcg
    16141 atgttgccga agagcacggc caggccaccc tctttcgagt aggcgttgga cttgtcgcga
    16201 atacagccac gcgtccggtc aagatcgagt tccgggtaac gacgattctg cgagaacgct
    16261 gtctgggtcg gaactccgcc gggtgaagcg cgaaagtatt cgtaaacctt gaggtcgcga
    16321 gaatgatgca tgacgtccca gtgcatgatg gcttcatcca gcgtcctgct gtgtacagtc
    16381 ggcacaccga ggtgcagcag cccggaccgt ttgagttcgc cgaggatgcc gtagatgcct
    16441 ccggcgcgat gcacatcctc gatgtgatac ttgtctgtca ttggagccac cttgcacagg
    16501 caaggtacct tgcgcgagat ccggtcaatg tcctgcatcg tgaaatcgat gccggcttcc
    16561 tgtgccgctg ccagcagatg cagaacggtg ttggttgagc cacccatcgt gacgtcgagc
    16621 gtcatggcgt tctcgaaagc ttcaaaggag ccgattgatc gcggcagcac ggacgcatcg
    16681 tcctgctcgt agtagcggcg gcagagttcg acgatctggt gtccggcctt gacgaaaagc
    16741 cccttgcgat cggcgtgggt ggccaccagc gttccattgc ccggcaatga caagccgagc
    16801 gcttccgtga ggcagttcat tgagttggcg gtaaacatgc cggagcagga accgcaggtc
    16861 gggcacgccg agcgttcgat ctcctcaaga tcggcatccg aaatcgtgct gtcgaccgct
    16921 ttgaccatcg cgtcgaccag atcgatgctg atcgtcttgc cgcccaactt gaccttgccg
    16981 gcttccatcg ggccgcccga aacgaagatg gccgggatgt tcacacgcat ggccgccatc
    17041 agcatgcccg gagtgatctt gtcgcagttc gagatacaga ccatggcgtc ggcgcaatgc
    17101 ccattgacca tgtattcgac agagtcggcg atcaaatcgc gcgaaggcag cgagtacagc
    17161 atgccgtcgt gccccatggc gatgccgtcg tcgatggcga tggtgttgaa ttccttggca
    17221 ataccaccgg ccgcttcaat ctcacgcgcc accagttgtc ccaaatcctt aaggtgtacg
    17281 tgccccggca caaactgggt gaatgaattg accacggcga tgatcggttt gccgaaatcg
    17341 ccgtccttca tgccggtcgc gcgccacagg gcgcgagcgc cggccatgtt gcggccgtgg
    17401 gtcgacgtgc gggaacgata atcaggcatg gggaatctcc agaacaggat ttggctgaat
    17461 ggtgcaagct ataatcggga ttctatccga aagcttcatc agcgttcgtc acgagttgcg
    17521 ttcaggctgc gttgcccgac tgatttccag acatgttgat ccatcctcag tttgaccccg
    17581 tcgccctgtc tttaggtccc ctgtccattc attggtacgg cttgatgtat ctggccgctt
    17641 tcttccagtt ctggtggctc ggcaagcggc gaatccaaac acacccgcaa ttgtcaaaat
    17701 ccggctggac ggtaccgcag cttgatgatc ttctgttcta cggcgtgctc ggcgtcatcc
    17761 ttggtggccg cctggggcag gtaatctttt acgagcccgg ctattacctt tctcatccgt
    17821 tggaaatcct ggcagtctgg aaaggcggca tgtcctttca cggcggcttc ctcggcgtgc
    17881 tggtggctat gggcttgttt gcccgcagga ctcagcgcac ttggcttgac attactgatt
    17941 tcattgcgcc gctggtgcca ttgggtctgg ccgcagggcg tatcggaaac ttcatcaatg
    18001 gtgaattgtg ggggcgcatc agcgatccag ctttgccttg ggcgatggtt ttcccaaaag
    18061 tggacgattt gccgcgtcat ccgtcgcagc tttatcaggc tggtttggaa ggcgtgttgc
    18121 tgttcatcgt tctctggtat ttttcgcgcg gagtgcggcc tcgtggggcc gtctccgggg
    18181 tgttcctcat tggctacggc agcttacgct ttgttgccga gtttttccgt atgccggatg
    18241 atggtatctt tggcctctcg gaggtcatca gcatggggca atggctgtcg ctgccaatga
    18301 ttctgatcgg agtcgttatg cttgtccggt cgctccggcg aggcgtcgga gaagctgaca
    18361 gctaaagatc gtgaggttta gggatgacag aaggaattgt ttcgcgaggt attcagaagg
    18421 ttcgcaaaat gctgagcgaa cactcaagca ccggcgtgct cagtgaaagg cagttggccc
    18481 agatacgcga acagctcaag gaatgcgctg tgggtctggg tggtgaaatt tctgcccggc
    18541 agcgcgcggc ccgtcttgca gacacgtatc tgtcgctgaa tgatgccggg cgcgcggcgt
    18601 ttttgcatat cgtcgcgacg gaattcggcc cggacccgaa atcggtagaa aaggcacaca
    18661 ctcgatatca ggcggcgatc ggtaccgaca gccagtgggc cgcggagtct gcactgcggg
    18721 gcgaattgcg ctccatcccg ttgcgtatcc tgacccagtt caacgctctg ccacaagggg
    18781 tcaagtttct agtcgatatg cgtgccgatt tgttgcgcta cgttgatgca gatctcgcgc
    18841 tgcgctcgct ggaccgcgaa ctcgaatacc agttcggcgc ctggttcgat gtaggtttcc
    18901 tggagcttca gcgtatttcc tggaactcgc cggcgatatt gctcgaaaaa ttgatcgaat
    18961 atgaagcggt gcatgaaatc cgctcatgga gcgatctgaa gaaccgcctc gattccgaca
    19021 gacgttgtta cgccttcttc catccgcgca tgcctatgga gccgctgatt ttcgtcgaag
    19081 tggcgctggt cgatgagttg gccgacaacg tacaagcctt gcttgacgag cgtgcgccgg
    19141 tgttcgatgc gcagcgtgcg aagacggcca tcttctattc catctcgacc actcagccag
    19201 gtttgcgtgg cgtatctttc ggcaatttcc tgctcaagcg cgtcgtcgac gatctgaagc
    19261 gcgacttctc gcgtctggca acctttgcca cgctttcacc gattccttcg ttgcgtcgct
    19321 gggtggaaaa aaatcccggg gtctggcagc aggcattcac agaggatatg gttcagcgtg
    19381 tcgctcgcca tgttgggccc aaggggcctg tcatcgacag tgcggtgggc atcaaagcct
    19441 tgctggtgga tgatgcatgg gccgccaaca cgcgattggc gcgcgccttg cagccaggat
    19501 tggtccggat ggctgctcgt tacctcctgc acgcgaaggc cggtacgcgt ccgtacgatc
    19561 cggtggctcg tttccacctt ggcaacggcg cgcgcatcga acgcatcaac acgttggctg
    19621 acatatcgac caacggtttg caacaatcct acgggttgat ggtcaactat ctctacgatc
    19681 ccgacgccat tgaaaccaat ctggaggctt tttcgcgtga aggcgtggtc gcaacatcgg
    19741 gtaccgtaag gcgttcggcg caaacgacat agattcaggc gcttccggtt tttgcgtgtc
    19801 tgggaggata ctgacactca agaaccggtt tttattggat aattctctcc ctgtcccacc
    19861 atcatctaaa ggaaagcaca atgaacttat cgcacattga acatatcggt atcgctgtca
    19921 aaagtctgga cgaagcgatc ccgttctggg aaaagcagct tgggctgaaa tgctattcca
    19981 tcgaagaggt caaggaacaa aaggtccgga ccgcgttctt caagattggg caatccaaaa
    20041 ttgaattgct ggagtcgacc gaaccggacg gcccgattgg aaaattcatc gagaaaaagg
    20101 gcgaaggcat tcagcacgtg gcgttcgcag ttagcgggat tcaggacaac cttaccgaat
    20161 tggaaggaaa aggcgttcaa ttgattgaca aggcgccacg aaaaggagcc gaaggcctga
    20221 atatcgcctt cctgcatcca aaatcgacac atggcgtatt gcttgagctg tgcgaagatc
    20281 ccaagaagta gttcccatct gcgagtgtaa atggccgccc cgctcgggcg gcttcatcct
    20341 ttgttgaggt gaatcgacga tggctgagtc ttctgtgccg attggtcaag ctgacaacaa
    20401 agccgcattc gtcgacgcca atggcgcaac tgcatggctt gccgggcaac cgcaagcaaa
    20461 cgcgccggcc atgctggccg gactggtcgc gcagattcag gctttcaaca gtgcctttac
    20521 ggcacctcgc gaacgcttca agacgctgga agcactgcgc aagacgatct ttgccgtcag
    20581 ccaggaatgc cagcggcgtt acgagaacaa accgctgccg ctcttgccgg ccgaacagac
    20641 agcgttggac atgtcgcgtc ggctctggcg ctcctgcgcg gtgggttata tgcattgtct
    20701 gcgtgcctgc cttgaaaaag atgggtcaat cgcatcgcat ggtgcccagg tggcgcaccg
    20761 cgtgctggcg tgcttgcgca tggaacaaat gaattgctat ttcgccggtg ccgatcttga
    20821 cggcgagttc tggcgcaatc tgcattcagt ttgggtttcg gcagagcaac tcggaaaaac
    20881 cagtgatctg gtcgaagacc gcctgcttgg cgaaaccagc aagtcaacag ttcgcggtca
    20941 gtactgcatg gttctgacgc tgcatctcgc ccgtcctttt accctttccc gcgggcaact
    21001 cgcggccgca gtccgttggt tcgcgcgctg gcgtgagttg gcggcggtac tcgccgagcc
    21061 aaatcagaac ccgaaatcct gctgcattgc aatcaacctg tcgcaggacc ggccaattca
    21121 tgagagcgct caaggcgcca gcgtcggacg ctggctctcg ctcgatggtg tgttgcagaa
    21181 gatgcgcaag cggctcgaac tgttggccaa gggcgattcg ccggaagttc tcaagctcgg
    21241 cagcgggctt tcgtcggaag cctgtgtcgc acttctgacc acactttccg aacatctcag
    21301 gtatccacaa aagacgccaa tcggggccct cctggaggcg tcgccgataa cagttgccgc
    21361 tgggctggaa aacatcaatc gattgctcgg cggcaaagga ttaaaaatat ctgtccagca
    21421 cgcttcgcta ggtactcagc tcagccatca tgagcaagtc gccttgtttg gccgtgcagt
    21481 gcgcgatgcc gaggaaagtg gcgagtgtaa atccgagatc tggcagatca cgcaacaggc
    21541 gcccggcgtg ctgaatctgt tctgccctgc cggaaacagc gaagcgcgtc tggtccttgg
    21601 agggttgttg tgcgtcaagt tgccgcagca cgaacatttc accttggcaa cgataagcgg
    21661 cctgaatacg cgtgctgatg gtggcctgtg cgtcgccgcc agcttgtttg acggtgaacc
    21721 ctctccactg atcgccgagg tgcgagaaaa acctgctggc aagctttccc gtcatccggc
    21781 tttccttttg acgggcagag aggaaggaag cacgccgttg gttgtcttgc cgatagggct
    21841 gcccgctcgt gctttgtcgt tccgcttctt tgaggcgcgc gagcagtccc tgattgccct
    21901 gcgattggtg gagaatctcg ggcgcggtgg cgacatcgag cgctggtcgg ctgctatcga
    21961 cgcgtagcgc tcgggtcgct acgccggttg agagtaaaat ccccggcatg aatacgctcc
    22021 tcgtcctgac caacctgcct gatcgagcga ccgccgaaac tctggctgcc cggctcgtag
    22081 aagaccggct ggctgcctgt gtcaatattc tccccccttg ccattcgatc tatcgctggc
    22141 agggtgaggt cgagacggcc gatgaagtgc cgctgctcat caaaacgacc gaggcacgtt
    22201 acccggcgct cgaggaagcg atccgcgcgc gacaccccta tgaaaccccg gagatcatcg
    22261 ctttacccgt gttgcttggc ctgccaggct atcttgcttg ggtagcggca gaaacacaga
    22321 gcgacgactg gtccgcacca tgatgcgtca gctattgcgt tgcctgatga tttctctgct
    22381 gctctgcagt ctcgccaacg cagaggacct gctgcatcca ctggctgcat tcaagcccac
    22441 ggcacgcgcg cttgatggtc agacgattga agtgcgcttc gagatcgccc cggcgtatta
    22501 cctttaccgc gacaagttcc gcttcagcgc tgaaccgccc ggcgtgcagc tcggcacgcc
    22561 cattctgccg aaaggcaagg agaagaatga cgagactttc ggcaaggtcg tcgtctacta
    22621 cgatgaagta ttgattcgcc ttccggtcga gcgcaacagc tccggcaaac tgccgctcac
    22681 cctgaatatc acgtcgcaag gttgtgccga tgcgggtgtc tgctacccgc cgcaaaagca
    22741 ggcactgagc cttgaactgc ccgacccgtc ggcagtgccg cttgatacgc cgagcggtga
    22801 tgaatccggc cgaatcgcgc aactgctgaa aaacgccggt ttctggctga tcgttttaag
    22861 tttcttcggc ttcggcttgc tgctctcact gacgccctgc gtctttccga tgattccgat
    22921 tctttccagc atcattgtcg gtgccggacg cgatggacat ggtgtgtcgc acgctcgcgg
    22981 cttcgcgctc tcgctggctt atgttactgg aatggcgata acctatgcag cggccggtat
    23041 tgccgccggc ctgaccggta cactgatttc gacggccctg cagaactcat gggtgctcgg
    23101 cggtttcgcg ttgattttcg tcgtactgtc attttctatg ttcggctttt atgagttgca
    23161 gttccccact ttcctgcaaa gcaaagtgtc ggaagaagcg tcacatatca agggcggatc
    23221 actccccggc gtggcggcca tgggggtgct gtcggcgata atcgtcggcc cctgcgtcgc
    23281 ggctccgctg gccggtgccc tgctttatat cgggcagacc ggcgacgcgc tgctaggcgg
    23341 tttggcgctc tttttcatgg cgttcggcat gggcactccg ctgctcgccg tcggcctctc
    23401 ggcggggacg ttgctgccca aatcggggcc gtggatggag gcggtcaaaa aggcgttcgg
    23461 cgtgatcctg ctggcctccg ccgtatggac ggtttcaccg gtcattccgg tcgtggttca
    23521 gatgctggcg tgggcgctgt tactgatcat tccggcgatc tacctgcacg cgctcgatcc
    23581 gttgccagcg cacgcgaagg gctggcaacg tttctggaaa ggcatcggta tggtcatgct
    23641 gattaccggt gccgcgatgc tggtcggtgc acttgccggc gccaaggatc cattgcagcc
    23701 gctttccggg ttgcgtggcg gggcccagag cagcgaaatc agtcgtctgc cgtttgagcg
    23761 catccactca atcgccgaac tggatgcccg catccaatcc tctgccaagc ccgtcatgct
    23821 cgacttctac gcggactggt gcgtgtcgtg caaggaattg gaacgcttca ctttttccga
    23881 tattcgtgtg caacaaaaac tcgccggctg gaccctgttg caagccgatg tgaccgccaa
    23941 cactgaggag gacaaggcgc ttctcgcgcg cttcaagctt ttcggcccac ccggaatcat
    24001 ttttttcgat gccagcggaa aagaagacat gggggttcgg gtgatcggct ttcagggcgc
    24061 cgatgaattt ctggcaacac tttcacgctt gcgctgaaag atcagtcctc agccttcggg
    24121 tatcggctcg tgggcagaaa tggatgcggc gacacccgcc gtaagaatgc cgagtactgt
    24181 ctgcgcgaac tctgccgctt cccgcccgag gctggtcagg tatccgccgt cagcctgtgt
    24241 gaccaacccc ttggcgtgca ggcgtcgaac cgcagcaatc acctgcttgt cggccgtctt
    24301 gtgcaccttg ataccctgca ggccggtctc aagatcaaaa cggatcaggg tattgagttc
    24361 atgaaccaga tcggacgtat agggcatgac cactccttat cgcgcaaagc gccattctac
    24421 gcgcaacttc ccgcgagttt acgaactgca tgagagcatt gagcaacccg ctttgtggcg
    24481 agcccagtca ggacgcctgg cggcaaatgc ttcacaccca ggccgttcat cgtatggatt
    24541 gcgcaagaca tcgagcaact gatgaatcat ttccggatca ccgttttcgg cgccgtcaat
    24601 cgcttgctgc gccagacagt tgcgcagtac atagcgtgga ttgacagcgt tcatgagcgc
    24661 tatccgagat tcttcgcgaa tatcgtccga gcgcacacgg gaagccc
//
LOCUS       DAORPM010000005        22914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_366_length_22914_cov_12.993701, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000005 DAORPM010000000
VERSION     DAORPM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 22914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22914
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_366_length_22914_cov_12.993701"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLD53_00490"
     CDS             complement(<1..391)
                     /locus_tag="PLD53_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRC59409.1"
                     /translation="MKVGNELTHFSDSGRARIVDVSEKSSTSRVAIASGTLRMQAGTL
                     ERIRNGKIAKGNVLSVADVGAVMAAKRTSELIPMCHSLALTGVAVEFSEILVPDENGC
                     VGIRVVTSATCTGQTGVEMEALCAASVA"
     gene            complement(398..1444)
                     /gene="moaA"
                     /locus_tag="PLD53_00495"
     CDS             complement(398..1444)
                     /gene="moaA"
                     /locus_tag="PLD53_00495"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRC59410.1"
                     /translation="MNNLSTPPLLVADTLQRPLRDLRISVTDRCNLRCTYCMPREVFG
                     KDFVFLPRAELLSFEEIERVAGVFVQLGVQKIRLSGGEPLMRHGVEHLIEALSKLCST
                     EGQPVEIALTTNGLLLSRKVQALKNAGLSRLTVSLDGLSDAVFQRMSDSTASVTTVLD
                     GIEAAQRVGFSPIKINMVVKRGENEHEIIPLVRRFRNTGIVLRFIEYMDVGTTNGWRM
                     NDVVTAQEILALINNHYPIRQLDPNYRGEVAKRWMFADGGGEIGVIASVTQAFCHDCS
                     RIRLSTDGKLYTCLFASDGLDLRDQLRRGESDTPLTRFIIDTWQRRADRYSQIRHETT
                     ALPGRKIEMSYIGG"
     gene            complement(1535..2377)
                     /locus_tag="PLD53_00500"
     CDS             complement(1535..2377)
                     /locus_tag="PLD53_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC59411.1"
                     /translation="MLRQLHSLDRPALNDMGHPPLVFVHGGYTHSGCWDVNFLPYFQE
                     RGYNCYAIDLSGHGKSSGRDALNSFGLDHYADDVGQLVDSLDAAPVLIGHSMGAIVVQ
                     RYLEKSPATAVVMMAPVPPTGLAASGAQLAIRQPDFIFEADRAVRGEYTENTIKVMRE
                     VYFSPDADLEHFTQFQPMVQQESMLVLTEMMSLAMRLPKKRPRIPALVIGGQLDALFA
                     ANRLHFTASGWNAETCVIPRAGHMLMLDPQWVDVAQKIDAWLGASLELKGFGTGCARE
                     ALAA"
     gene            2587..3996
                     /gene="pssA"
                     /locus_tag="PLD53_00505"
     CDS             2587..3996
                     /gene="pssA"
                     /locus_tag="PLD53_00505"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:NF006946.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRC59412.1"
                     /translation="MERTQTRIPKTNGFTSVWQRWRGRTNETPSLDSLPCIPVAAEHV
                     AVLPSPEAYRTTLLTQIAQASQRILMATLYLQDDDAGREVLSALYAAKKARPQLEIAV
                     FVDWHRAQRGLIGKARSAGNAALYKDMARRLGPGVEIYGVPIQRREFMGVMHLKGFVI
                     DDVVLYSGASLNDVYLQRNSRYRLDRYHLIHNRFLADSMANLLTGVLRNGPSVCSLGT
                     GKTPKTAAILNAIVKFRRVLATIRYHFAAGQINSGEVGITPLFGLGVRGNELNATILK
                     LILHSRQRFVLFTPYFNLPGSIRKAVDSKIKAGCQVTIVLGDKTASDFYIPPDEPFKT
                     IGALPYLYEANLRRFCRTHQKAIDAGLLNVHIWRHEDNSFHLKGLLIDEDYALLTGNN
                     LNPRAWRLDLENGLVIHDPQKLLLDQHLAELARIMVHTRRLNHHKSLDAVDTYPLPVQ
                     RLIKRLARVRADRLVNQVL"
     gene            complement(4027..5163)
                     /locus_tag="PLD53_00510"
     CDS             complement(4027..5163)
                     /locus_tag="PLD53_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRC59413.1"
                     /translation="MERDQALKFMHDLLRLMSQKNGSDLFITANFPPAIKVDGKITPV
                     SNQVLTAQHTAELARSIMNDRQAAEFEATKECNFAISPSGIGRFRVNTLIQQGRVAVV
                     CRTINLSIPTLEELGLPTVLKDLAMTKRGLVIFVGGTGTGKTTSLAALVDHRNKNSHG
                     HIITVEDPIEFIHDHKGCIVTQREIGVDTDTWEAALKNTLRQAPDVILMGEIRDRETM
                     DHAVAFAETGHLCLATLHANSSNQAIDRIINFFPEERRHQLLMDLSLNLRALVSQRLL
                     PKKDGKGRVAAIEIMLNSPLISDLIFKGEVHEIKEIMKKSRELGMQTFDQALFDHYEA
                     GRITYEDALRNADSINDLRLQIKLHSKDSKDRDLNSGIGHLEVV"
     gene            complement(5188..6234)
                     /locus_tag="PLD53_00515"
     CDS             complement(5188..6234)
                     /locus_tag="PLD53_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRC59414.1"
                     /translation="MDITELLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINLPAMEH
                     KDVHSMVYDIMNDGQRKHYEEILECDFSFEIPNLARFRVNAFNQNRGAAAVFRTIPSK
                     IMSLEDLNCPKIFKDISEYPRGIVLVTGPTGSGKSTTLAAMVNHINENEHAHILTVED
                     PIEFVHESKKCLINQREVGPHTLSFANALRSALREDPDVILVGEMRDLETIRLALTGA
                     ETGHLVFGTLHTSSAAKTIDRVVDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGMG
                     RTAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGAQLGMQTLDQNLLELVRRNVVSAAE
                     ARGKAANKDAFPGA"
     gene            6303..6992
                     /locus_tag="PLD53_00520"
     CDS             6303..6992
                     /locus_tag="PLD53_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRC59415.1"
                     /translation="MTTISANLQAVLARIFAAARKYGRNPDDISLLAVSKTWPASVVR
                     EAAVAGQQAFGENYVQEGAAKIAELGSLGLVWHFIGPLQSNKAKQVATAFDWVHSVDR
                     LKIAERLSEQRPDSLAPLQVCLQVNVSGEKSKSGTLMDDLSSLAHRVALLPRLKLRGL
                     MAIPAPVEDFAQQRKSFRRLREAFERLKESGLLLDTLSMGMSHDLEAAVAEGASIVRV
                     GTAIFGARSKS"
     gene            6989..7801
                     /gene="proC"
                     /locus_tag="PLD53_00525"
     CDS             6989..7801
                     /gene="proC"
                     /locus_tag="PLD53_00525"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRC59416.1"
                     /translation="MKITFLGGGNMASALIGGLLNTGFPPGDITVIEISADNRARLER
                     DHKVRCYSELQAVAFQCDALLLAVKPQQMKEACTPLREHLQQQLVISIAAGLRLADLS
                     RWLGGYDRLVRAMPNTPALIGSGMTGLFALPTVTEAERLGAERILRAVGSAVWVADEA
                     QMDAVTAVSGGGPAYVFLFIEALQQAAMDLGLTSRQARQLAIETTLGAAKLAAQSDDS
                     PCVLRERVTSKGGTTEAALLAMAERGVKDSIIAGVLAANARGAELGELLGKD"
     gene            7804..8343
                     /locus_tag="PLD53_00530"
     CDS             7804..8343
                     /locus_tag="PLD53_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRC59417.1"
                     /translation="MLTEAGLFLLNALIGFLTFALLLRFYFQAFRVSFRNQIGAFVVQ
                     LTNWLVMPLRKVLPGFFGLDLASLLPAYLLQVLLLAAAISLRGTVGLPPAGALVGIIL
                     WQGVLATLRFSIYLLIGALFLQAILSWVSPYSPLVQPVSQLTRPFLRPIQRIVPPIAA
                     IDLSPLVAILLAQLVLMFL"
     gene            8343..8636
                     /locus_tag="PLD53_00535"
     CDS             8343..8636
                     /locus_tag="PLD53_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRC59418.1"
                     /translation="MCPWLLVSDGKITLKLHIRPGAKQTAVTGLHGEALKIRLAAAPI
                     DGQANAALIAFVADRLGLPKSAVCLRSGQTSRRKVLEVIGAPADAAQRLLPIS"
     gene            complement(8629..9924)
                     /locus_tag="PLD53_00540"
     CDS             complement(8629..9924)
                     /locus_tag="PLD53_00540"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRC59419.1"
                     /translation="MHSEFSRFELPHIQIKNGRVIDPKNGIDEQADVFVADGRIQAIG
                     QPPSGFKPAQVLDASGRIVCPGLVDLSTRLANLESELSAAVAGGVTAVACPPDTKPPL
                     DEPGLVERLVRRSEALGLARVFPIGALTQQLAGEKLSEMVSLTRAGCIAFSQAKQPIF
                     DTQVLLRAMQYAATFGYATRLQPQDHYLARDGVAHDGEVASRLGLAGIPVCAETVAIV
                     TALQLAQVTGVRLHLARLSSAAGIALVRQARNNGLNISCDVSIHHLHLCEADIGYFDS
                     HARFDPPLRSESDRDALRAAAADGLATICSDHTPVDEDGKQLPFAESLSGATGLELLL
                     PLVLKWAVEMKLPLLTAIARVTCDPAAILGIEAGSLSAGCPADICVFDPTETWCVKPM
                     ALRSEGKNSPFMDSEMTGRVHTTLVGGRIVFEIQESRFS"
     gene            complement(9928..10902)
                     /locus_tag="PLD53_00545"
     CDS             complement(9928..10902)
                     /locus_tag="PLD53_00545"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRC59420.1"
                     /translation="MLSSLHKNPQLNTNGELTHLLSIHGLPVEILTQILDTASSFLKV
                     SDRDVKKVPLLRGKSVFNVFFENSTRTRTTFEIAAKRLSADVINLDVTRSSTAKGETL
                     LDTVDNLVAMHADMFVVRHAASGAPYLIVDHLRRVGRTDVHVVNAGDGRHSHPTQGLL
                     DMYTIRHYKKGFTNLTVAIVGDILHSRVARSDIHALKTLGVAQIRAVGPQTLMPADVE
                     KMGVRVCNDLQEGLRDCDVVIMLRLQNERMNGVLLPSAREYFSSYGLTPETLALAKDD
                     AIVMHPGPMNRGVEIDSEVADGPQAVILPQVTFGIAVRMAVMSILAGN"
     gene            complement(10895..11401)
                     /gene="pyrR"
                     /locus_tag="PLD53_00550"
     CDS             complement(10895..11401)
                     /gene="pyrR"
                     /locus_tag="PLD53_00550"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRC59421.1"
                     /translation="MPLTKLPDAEAQCAELAALIRTQLKANTMLVGIHSGGVWVARRL
                     HELLGLAGEIGQIDVSFYRDDFGEKGLHPQVKPTSIPFDVEALHLILVDDVLYTGRTT
                     RAAVNELFDYGRPASIKLAVLADRGGRELPICAEFCTWQVDLEENEELVLEPEATGRL
                     QWKRVEHA"
     gene            complement(11379..11873)
                     /gene="ruvX"
                     /locus_tag="PLD53_00555"
     CDS             complement(11379..11873)
                     /gene="ruvX"
                     /locus_tag="PLD53_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRC59422.1"
                     /translation="MPDATTSTRGTVLAFDFGEKRIGVAVGECLLGQAHPLTTIRSEV
                     NVERFAAIAALIDEWQPVRLIVGLPVALDGTTHAMTARCTRFANQLRGRFGIAVDYAE
                     ERLSSVEAEERLRESGHNAKSAKEHIDALAAQIVLQGFFERSAKQPVDDPTDHKIYAT
                     HQVA"
     gene            complement(11866..12426)
                     /locus_tag="PLD53_00560"
     CDS             complement(11866..12426)
                     /locus_tag="PLD53_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRC59423.1"
                     /translation="MQSFDLTDHFLIAMPAMDDPYFSKSLIYIAEHNDQGALGIIVNR
                     PIDMSLATLFEKIDVPFETLGMANLPIFFGGPVQTDRGFVLHRPVGDWQSTLAVNQDV
                     GLTSSRDVLQAVARDGQPHEIMVTLGYSGWGAGQIEHELAQNAWLTVPADPHILFELP
                     YEDRLPSAMEILGIDFKNLAVKAGHA"
     gene            complement(12683..18403)
                     /locus_tag="PLD53_00565"
     CDS             complement(12683..18403)
                     /locus_tag="PLD53_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRC59424.1"
                     /translation="MNAATEFDVGPLTWVKSEIDLALERADQALQQFSASVVAGSGDL
                     TQLRFSRTHLHQVQGALTIVGLDGVTQFAEAVESLLEGIEQQKRPADDDFVALAQRSL
                     AAISHYLGDLISGQPNQPLRLLPLYREVQEARGLEHVSATDLFFPDLTARLPRRETPA
                     RKIAHSDLQRLARQERARFQHGLLAWLRAPHDSRGISEMLGAIKRIENTQDVPSARAF
                     WWITSGFLSALAEDSLPGETNVKQLCARIDLQIRRLIEGSKNVAERLMRDVLYFVASA
                     DSNNDTVRLVKKTYQLHAIIPTTATSAPAPEEAVRRKLRDVITLTEEAWNKYCTGTMQ
                     SLPLFREHANALSAVVEQLGHTDFRRLAQAIAAAANWLSEGSSRHSESLAMEIATAIL
                     LTQNAQENFEQLGNDFAHQVDVTVARIHGCIAGTPPQPGSEVPLLDEMSRKAQEKLLV
                     GQVAKEIQSNFAQIEQVLDGFFRDTSKSADLVSLEVPFRQVVGALTMMRHDAAAVAVR
                     ECSEEIKRFAEPESPSQEADFERVANQLSMIGFFIDSLQHGAVDFEGFVRQMQSGTAL
                     EAEETGELDEFEGIDESGPFEEIGTFEMAGDAEVPKSDISVEQEVERLKLETHALLGA
                     LKEQAGNENLIEQVRHNLSALKNDADLVADTALGEQAKAMLTAFKAGGVASPQIDQFM
                     ATLKPELPESMKSSAETVQLSQASNEEIDAELLDIFLEEANEVLGTIDENLQALKGQP
                     HNVELLTTIRRSFHTLKGSGRMVGLTDVGEAAWSIEQTLNLWLRQELEVGASIFDLLE
                     QAHSVFATWVRYLETQTGSAPDPTDLITFSDSLRREKEEVPEAPPAPTVDATASSEVE
                     GELETISLDELPSETPLESEESPEALQSAELIEFDAPQPPPEQEGANLASDLEIVEEI
                     DESDFSFDTPVEEAAAQAEEGALPDVEPPAVEPVASESEAQLRISISPTLYEIFSEEA
                     DVHLATLHRELPVLEKDELTPTPQDMYRAAHTLAGISATVGISSVNQLGLALEHALLR
                     RDNSAQPGSLEALGIVRQAVGELDLMLSSLADEREPDVPAGLIEALDSLYPANLVTSQ
                     EPPAEHEAATEQLASAETAPPQETAKAEFEHKPSFLSEIKLLKDEIDEQLLPIFLEEA
                     VDLNQGIATQLRALRTEPNSAEAFRTLKRQLHTLKGSARMAGAMNLGEITHAIETRVD
                     QASSADGVPLEVIDEIESAFDSVLHIIERLQKGEKLDQLPETPAAPHVPVDDAALAKP
                     GAEPPEKVAVTVERRTDRRGDRLRVAEAEVDAEALAQRATLRVRADVIDRLVNEAGEL
                     SIARARIEGEMRSLKTSLLDLTENVIRLRRQLREVEIQAESQMQSHTAQTNEQHAGFD
                     PLELDRFTRFQELTRMMAESVNDVATVQQNLLKNLDDANASIIAQARLNREVQQALMA
                     VRMVPFGSLADRLYRIVRQTSKELNKRANLEIVGSQVELDRSVLDKMGAPLEHLLRNA
                     IAHGLEEREIRVAQGKPGIGEISLALKQEGNEIILSLTDDGAGLDYERIRARAIDAGL
                     LTADEEASPTRLADMIFTSGFSTATDVSQIAGRGVGMDVVKTEVSRLGGRIEIVSASG
                     QGTQFLLYIPLTLAVTKALLVRAGNKNYAIPSVMIEQVLDLKEDALSRIRDAGSAEWM
                     GNQYPFNYLPHMLGEIQTHPDHQRQYWVLLLRSGAQRIAVQVDELLGNHEIVVKNIGP
                     QLARVIGIDGATVLGNGQVVLILNPVALSRRSVVELAAPISTTPELPAEETATATLPK
                     VMIVDDSLTVRKITGRLLAREGYQVLTAKDGVEALEQLLDVVPEVMIVDIEMPRMDGF
                     DLTRNIRADSRLKAIPIIMITSRTAEKHRAYAFEIGVNNYLGKPYQEDELLQLVADFV
                     SQQRHA"
     gene            complement(18437..20584)
                     /locus_tag="PLD53_00570"
     CDS             complement(18437..20584)
                     /locus_tag="PLD53_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRC59425.1"
                     /translation="MAFNLKLSGSNDAAGQTASDTTIMDDAQPIATKKSASGPGFLGQ
                     YSVIKQLQILGGAFLLIVVIIIAFVYRDNRESAFNSTYIATAGEMRMLSQRLAKASSL
                     ALQGNTSAFKQLRDSHSQFVTNLERLTKGGELAGTTVPPSPDDVQPQLQALTKTWSAT
                     DKDAVQLLDMETNLVALSKDVASITNKNPEMLELSEQVAALKLQSGAGVREIAAANQM
                     VMLTQRIAKNASSLLVGDEISPEIAFLLGKDTNTFRELLNALQKGNENLRISGTTDAD
                     TKRKLGDLETSFKEYQDAVGGILGSMQRLTLAKRAGSSIFRNSEGLLESTDNLANAYQ
                     QGGRLAYGVTFAVLMFLALATLAMMAKVYLDDSRRQAETANESRQATESINRQNQDAI
                     LRLMNELGDLADGDLTVTATVSEDITGAIADSINYTIEELRVLVRRINDAAGRVTTAT
                     GIAQQTSVELLEAAQRQSEEIQAAGLSVLAMASSMTKVSGDADQSAQVARQSLAAAGK
                     GTQAVQDSIKGMTEIREQIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAA
                     IQAASAGDAGRGFTVVAEEVQRLAERSGEATKQIAAIVKTIQTDTQDAVSAMEESTHG
                     VVEGARLSDATGQALAEIGSVSQNLAELIENISGATRQQADSATSVAGKMQDILRVTE
                     QTTAGTQQTATAVGELADLATELKGSVAGFKVG"
     gene            complement(20652..21161)
                     /locus_tag="PLD53_00575"
     CDS             complement(20652..21161)
                     /locus_tag="PLD53_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRC59426.1"
                     /translation="MAKKGSLREFQAHLAKRLAGVSDQSAAGLLGVQAGSDFWLISLF
                     DSGEIIPLTPLTGVPLTKPWFAGIANIRGNLYAVADFSAFQGKEATPQNASSRLLLIG
                     TRHGDNAALLIPRVLGLRNLDDLTPVATDPDAPPWSSEAYSDNEGRRWNMLNVRRLLS
                     DDSFMDIGV"
     gene            complement(21177..21539)
                     /locus_tag="PLD53_00580"
     CDS             complement(21177..21539)
                     /locus_tag="PLD53_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC59427.1"
                     /translation="MSIKKILVVDDSPTERHAMLELLSKHGYQVITAESGEEGIAKAK
                     AELPDLVLMDVVMPGLNGYQATRTLTRDEATKHIPVIVCTSKGQETDKIWGLRQGAQD
                     YLVKPVNSEELLAKIAAL"
     gene            complement(21582..21941)
                     /locus_tag="PLD53_00585"
     CDS             complement(21582..21941)
                     /locus_tag="PLD53_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC59428.1"
                     /translation="MIIDDSNTIRRSAEIFLVQAGCQVVLAEDGFDALAKIADHQPEV
                     IFCDIMMPRLDGYQTCALIKKNQRFRNTPLIMLSSKDGLFDRARGRMVGSDQYLTKPF
                     TKDSLLQAVATFARQPD"
     gene            complement(22085..22237)
                     /locus_tag="PLD53_00590"
     CDS             complement(22085..22237)
                     /locus_tag="PLD53_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRC59429.1"
                     /translation="MCLICGYVYDEAAGSPEEGIPAGTRWRDIPINWVCPECGARKDE
                     FEMLEI"
     gene            22398..>22914
                     /locus_tag="PLD53_00595"
     CDS             22398..>22914
                     /locus_tag="PLD53_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HRC59430.1"
                     /translation="MQLPPPIVLTFAASDPTGGAGIQADLMTVTSLACHALSVITALT
                     VQDTLGITSVLPVNADWVEAQARTLLSDMPVACFKIGVLGSVENIRAVARILSDHPDL
                     PVVFDPVLASGRGDQLADESMVLALTELLLPLVSILTPNSLEARWLAGMTNAEDCESD
                     MRLEECAHRLID"
BASE COUNT         4666 a         6539 c         6525 g         5184 t
ORIGIN      
        1 gcgcaacgct tgccgcgcac agcgcttcca tctcgacccc ggtctgcccg gtacacgtgg
       61 cggaagtcac caccctgata ccgacgcatc cgttttcatc cggtaccagt atctcgctga
      121 actcgaccgc gacgccggtc agtgccaacg agtggcacat cggaatcaac tccgaggtac
      181 gtttggccgc catgacagca ccaacgtcgg ctacgctcag cacgttgccc ttggcgatct
      241 tgccgttgcg gatgcgctcc agcgtgcctg cctgcatgcg tagcgtcccg ctggcgatgg
      301 cgacacgcga agtgctggat ttttccgata catcgactat gcgcgcacgt ccggaatcgg
      361 aaaagtgagt cagttcgtta cctaccttca cccgaagtca tcccccaatg tacgacatct
      421 cgatcttgcg tcccgggagt gccgtggtct cgtggcgtat ctgcgaatag cggtcggcac
      481 gacgttgcca cgtatcaatg atgaatcggg tcagcggcgt atcgctctcg ccacgtcgta
      541 gttgatcgcg caaatcaaga ccatcgcttg cgaaaagaca cgtgtagagc ttgccatcag
      601 tggatagcct gattcgactg caatcatgac agaacgcctg cgtgaccgat gcaatcacgc
      661 cgatttcacc gccgccatcc gcaaacatcc agcgttttgc cacttcaccg cggtagtttg
      721 gatcaagctg acgaatcggg taatgattgt tgatcaacgc gagaatctcc tgtgccgtca
      781 ccacatcgtt catgcgccag ccgttggtgg taccgacgtc catgtactcg ataaagcgca
      841 gaacaattcc ggtgttgcgg aagcggcgaa ccagcggaat gatttcatgc tcgttctccc
      901 ctcgcttgac gaccatattg atcttgatcg gcgaaaaacc gacgcgttgc gccgcttcga
      961 taccgtcgag taccgtcgtc accgatgcgg tcgaatcgct catgcgttga aagacagcat
     1021 cactcaagcc gtcgaggctg acggtaaggc gcgacaagcc agcattcttc agcgcctgaa
     1081 cctttctcga gagcaacagt ccgttggtcg tcaaggctat ttcgacgggc tggccctccg
     1141 tagaacacag cttcgacagc gcctcgatca ggtgctcgac accgtgacgc atcaacggct
     1201 cgccgccgga caggcggatc ttctgtacac ccagttgcac aaacacgccg gcgactcgtt
     1261 caatctcctc gaagctgagc aattcggcgc gcggcaagaa gacgaaatcc ttgccaaaga
     1321 cctcacgcgg catgcagtag gtgcagcgca gattacagcg gtcggtgact gaaatacgca
     1381 ggtcacgcag tggccgctgc aacgtgtcgg caaccagcag cggtggcgtg ctgagattat
     1441 tcacatggaa tccagaaccg ggccgaaaat ggtgtcagaa gaagcaggga agaggctggt
     1501 gcgcagacgt gagccggcga gctcccgtcg ggctttacgc ggccaatgcc tctcgggcac
     1561 atcctgtgcc aaatcccttc agctcaaggc tggcaccaag ccaggcatcg atcttctggg
     1621 ccacatccac ccactgcgga tccagcatca gcatgtgccc cgcacgcgga atgacgcagg
     1681 tttccgcgtt ccagcccgac gctgtaaagt gcagccggtt tgccgcaaac agcgcatcca
     1741 gctgaccacc gatgaccagt gcgggtatac gcggtctctt cttgggtaag cgcatggcaa
     1801 gcgacatcat ctcagtcagc accagcatcg attcttgctg gaccatcggc tgaaattggg
     1861 tgaaatgctc caagtctgcg tctggcgaga aataaacttc gcgcatcacc ttgatcgtat
     1921 tctcagtgta ctcgccgcgc actgcacgat cagcttcgaa aataaagtcc ggttgacgaa
     1981 tcgccagttg agctcctgat gcggccaggc cggtcggcgg aacaggtgcc atcatcacta
     2041 ccgcagtcgc cggtgacttt tcaagatatc gctgcaccac gattgcgccc atcgagtgcc
     2101 cgatcaatac cggagcggca tccagactgt cgacgagttg cccaacgtcg tcggcgtagt
     2161 ggtcaagccc gaagctgttc agcgcatcgc gccccgagct cttgccatgc ccggacagat
     2221 ctatcgcata gcagttatag ccccgctcct ggaaataggg tagaaagttt acatcccagc
     2281 atccggagtg cgtatagcct ccgtgcacaa acaccagcgg tggatggccc atgtcgttca
     2341 gcgcgggacg atcaagcgaa tgaagttggc gaagcattct gaaaactccc agagttagac
     2401 caatccgaga gaatagggca aaatacaggg ttcgacaata cggccaagtc aaatgcgtga
     2461 aggtaaagca cgctattcgc ggtataccca tttgctcggc caagaactcc caccgagtca
     2521 gatgggcatc gaatcgatgc gctgcgattg atttgcccac taaacctacc gatataatcc
     2581 ttttccgtgg aacgcaccca gacacgtatc cccaagacca acggcttcac ttcggtgtgg
     2641 caacgttggc gtgggcgtac caacgagacg ccgtcgcttg acagtttgcc ttgtatcccg
     2701 gtcgctgccg agcacgtcgc ggttctgccg agcccggaag cgtaccgcac cacgctgctg
     2761 acgcagatcg cacaagccag ccagcgcatt ctcatggcaa cactgtacct gcaggatgat
     2821 gacgccggcc gggaggtgtt gtcagcgctg tacgcggcga agaaagcgcg gccacagctg
     2881 gagattgcag tctttgttga ctggcatcgc gcacagcgcg gtctcatagg caaggcccgg
     2941 tcggctggca acgccgcact gtacaaggac atggcgcgtc gccttggccc gggcgtcgag
     3001 atctatggcg ttccaatcca gcggcgggag ttcatgggcg tcatgcacct caaggggttc
     3061 gtcattgacg atgttgtttt atacagtggc gccagtctga acgacgttta tctgcagcga
     3121 aacagtcgct atcgtctcga ccgttaccac ttgatccaca atcggttctt ggccgacagt
     3181 atggccaact tgttaaccgg agtgttgcgt aacggcccgt cggtgtgctc tctgggcacc
     3241 ggcaagacgc ccaagaccgc agccatactg aacgcgattg taaaatttcg ccgtgtactc
     3301 gccacaatcc gctatcactt tgcagcggga cagatcaatt cgggagaagt cggcattacg
     3361 ccattattcg gcctcggcgt tcgcggcaac gaactcaatg cgacgatcct gaagttgatc
     3421 ctccactcaa gacagcgctt tgtcttgttt accccttatt tcaatcttcc cggctcgatc
     3481 cgcaaggcgg tcgacagcaa gatcaaggcg ggatgtcagg tcaccattgt gctcggcgat
     3541 aaaacggcca gcgattttta cataccaccc gacgagcctt tcaaaaccat cggcgcacta
     3601 ccctacctct acgaagccaa ccttcgccgt ttttgccgga cccatcagaa ggccattgat
     3661 gcggggctgc tcaacgtgca catctggcgc cacgaagaca acagtttcca tctcaagggt
     3721 ctgctgatcg acgaagacta tgcgctgctg accggcaaca acctcaaccc gcgcgcctgg
     3781 cgactcgatt tggaaaacgg gttggtgatt cacgatccgc aaaaactgct tcttgaccag
     3841 catctggccg agctggcaag aatcatggtc cacacgcgac gactgaatca tcataagtcg
     3901 ctggatgccg tcgataccta cccgcttcct gtccagcgcc tgatcaagcg actggcgcgg
     3961 gtgcgcgcgg atcgtctggt caatcaggtg ctgtagtcct aagcgcttag gccacgcttg
     4021 ggcaggctat acgacttcga gatgccctat ccccgagtta aggtcgcgat cttttgagtc
     4081 tttgctgtgc agtttgattt gcaggcgaag gtcattgatc gaatcggcgt tgcgcagggc
     4141 gtcctcgtag gtaatgcggc ccgcttcata gtggtcgaac agcgcctggt cgaaggtttg
     4201 catgccgagt tctcgcgact tcttcatgat ctccttgatt tcgtgcactt cgcctttaaa
     4261 gatgagatcg gagatcagcg gggaattgag catgatttca atagccgcca cgcggccctt
     4321 gccgtctttt ttgggcagca gacgctgcga gacgagggcg cgcaggttga gtgataggtc
     4381 catcagaagc tggtgacgac gctcttccgg gaagaagttg atgatccggt cgatggcctg
     4441 attggagctg ttcgcgtgta gcgtcgccag acagaggtgt ccggtctcgg caaaggcaac
     4501 cgcgtggtcc attgtctcac ggtcacggat ttcgcccatc agaatgacat cgggcgcctg
     4561 acgcaaagtg ttcttcaacg ccgcctccca ggtgtctgta tcgacgccga tctcgcgctg
     4621 tgtgacgatg cagcctttgt gatcgtggat gaattcgatc gggtcttcga ccgtgatgat
     4681 gtgtccgtga ctattcttgt tgcggtggtc gaccagtgcg gccaaagaag tggttttccc
     4741 cgtacccgta ccgccgacaa aaatgaccag accgcgtttg gtcatcgcca gatctttcag
     4801 caccgtaggc aatccgagtt cttccagcgt cgggattgac aggttgattg ttcggcagac
     4861 aacggccaca cggccctgct gtatcagcgt attgacacga aaacgtccga tgccggaggg
     4921 tgagatggcg aaattgcatt cctttgtggc ttcaaattcg gccgcctggc gatcgttcat
     4981 gatggaacgg gccagctcag ccgtgtgctg cgcagtcaat acctggttgg agaccggggt
     5041 gatcttccca tccaccttga tcgcgggagg gaagttggcc gttatgaaga ggtctgaacc
     5101 attcttctgc gacatcaggc gcaaaagatc gtgcatgaat tttagtgcct gatcgcgttc
     5161 catgatttac ccctctaagc tgacgaatta tgcgcccggg aaagcgtcct tgttggcggc
     5221 cttgccgcgc gcctcggcgg cggaaacaac gttccggcgg acgagttcaa gaagattctg
     5281 atccagcgtt tgcataccaa gctgtgcacc cgtctggatg gccgaataca tctgagccac
     5341 cttgttttca cggatcaggt tacgaatcgc cggcgtgccg atcatgattt cgtgcgcagc
     5401 cgttcgcccc atgccatcct tggtcttgag caaggtctgg gaaatgacgg cacgcagcga
     5461 ctccgagagc atcgagcgaa ccatttcctt ctcggcagcc gggaagacgt caacaacgcg
     5521 gtcaatcgtc ttcgcagcac tcgaagtgtg cagcgttcca aaaacgaggt gaccggtttc
     5581 ggcgcctgtg agcgccagcc ggatggtctc cagatcgcgc atttcgccaa ccagaatgac
     5641 atccgggtcc tcgcgcaacg ccgaacgcag ggcgttggcg aacgaaagtg tatgcggccc
     5701 gacttcgcgt tgattgatca ggcatttctt ggattcgtgc acgaactcga tcggatcctc
     5761 gacggtaagg atgtgggcat gttcgttttc gttgatatgg ttgaccattg ccgccagcgt
     5821 cgtcgatttt cccgaaccgg tcggaccggt taccagaaca atgccgcgag gatactcgga
     5881 aatatccttg aatatcttcg ggcagttcaa atcctcaagc gacatgatct tggaaggaat
     5941 tgtgcggaac accgcagccg ctccccggtt ctggttgaac gcattgacgc ggaagcgtgc
     6001 cagattgggg atttcaaagg aaaaatcgca ctccagaatt tcctcgtaat gcttgcgctg
     6061 gccgtcattc atgatgtcgt acaccatgga atgcacgtcc ttgtgctcca tggcgggcaa
     6121 gttgattcgg cgtacatcac catgcacccg gatcatcggc ggcagacccg acgacaagtg
     6181 caggtcagac gctttgttct tgacactaaa ggccagcagt tcggtgatat ccatcggggt
     6241 tcgtcctcag cgggggtcag ggtgcggtgt tatacttagg aacatccggg tgacgtttga
     6301 ttatgacaac aatttccgct aacctgcaag ccgttctcgc acgcattttc gcggcagctc
     6361 gaaaatacgg acgaaaccct gacgatatta gtctgctcgc cgtcagcaag acgtggcctg
     6421 cgtcggttgt ccgggaggcg gcggtggccg gacaacaagc cttcggcgag aattatgtgc
     6481 aagagggggc agcgaagatc gccgagcttg gctcgcttgg actggtgtgg cacttcatcg
     6541 ggcctctaca aagtaacaag gcgaagcaag ttgccacagc attcgactgg gtgcattcgg
     6601 tcgatcgcct gaagattgca gagcggctct ccgagcagcg cccagactcg ctcgcgccct
     6661 tacaggtttg cctgcaggtg aacgtcagcg gtgaaaaatc gaagagcggc acgctgatgg
     6721 atgacttatc gtcattggcg catcgggtgg cgttgcttcc tcgcttaaag ctgcggggtt
     6781 tgatggccat tcccgcgccg gttgaagatt ttgcacagca acggaagtcg tttcgccggc
     6841 tgcgcgaggc atttgaacgc ctcaaggaga gtggtctgct gctcgacacc ctgtcgatgg
     6901 gcatgtctca cgaccttgag gcggcagtag ctgaaggcgc gtcaatcgta cgtgtaggta
     6961 cagcaatttt tggtgcaaga agcaaatcat gaaaattaca ttcctgggtg gtggaaacat
     7021 ggccagcgcg ctgattggcg gcttgctgaa tacggggttt ccaccgggcg atatcaccgt
     7081 catcgagatc agcgccgata atcgtgcgcg tcttgaaaga gaccacaagg tccgatgtta
     7141 ttccgagctt caggccgtcg cctttcaatg cgacgccttg ttgctggccg tcaagccgca
     7201 acagatgaaa gaagcctgca caccgctgcg agaacatctg cagcagcagt tggtcatcag
     7261 catcgccgcc ggcttgcgtc ttgccgatct gtcgcgctgg ctaggcgggt acgacaggct
     7321 cgtgcgggcc atgcccaaca ccccggcact gatcggttcc ggcatgaccg ggctgttcgc
     7381 cttgccaacc gtcaccgaag ccgagcgact cggtgccgag cgtatcctgc gagctgtcgg
     7441 tagcgcggta tgggtcgctg atgaggcgca gatggacgcc gtaactgccg tttcgggtgg
     7501 tggcccggcg tatgtctttc tgtttattga agctttgcag caggcagcaa tggatctcgg
     7561 cctgacatca aggcaggcgc gccagctggc aattgaaacg acgctgggtg cagccaaact
     7621 ggccgcccaa tccgatgatt ctccctgcgt cctccgcgag agagtcactt ccaaaggcgg
     7681 gacaaccgaa gcggcattgc tcgcaatggc cgagcggggc gtcaaggata gcatcattgc
     7741 tggtgtgctt gcggccaatg cgcggggagc cgaattgggc gaattgctcg gcaaggacta
     7801 gccatgctaa ccgaggccgg actattcctg ctcaatgcgc tgatcggttt tctcactttt
     7861 gcattgctgt tgcgatttta cttccaggca ttccgcgtct cattccgcaa ccagatcggt
     7921 gctttcgtcg tgcagttgac aaactggctg gtaatgccgc tgcgcaaggt tttgccagga
     7981 tttttcggcc tcgatctggc cagcctcttg ccggcgtatc tgttgcaggt gctactgctg
     8041 gcggcagcga tttctttgcg tggcacggtt ggtctgccac ctgctggcgc gctggtcggc
     8101 atcatcttgt ggcaaggcgt tttggcgacg ctgcgcttca gtatctacct tttgatcggt
     8161 gcgctcttcc tgcaggccat tctgtcgtgg gtgagcccct actcaccgtt ggtccagccg
     8221 gttagccagc tgacacgccc tttcctgcgc ccgattcaac gcatcgtccc gccgattgcg
     8281 gcaattgacc tctcgccact ggtcgcgatt ctgcttgctc agctcgtact gatgtttctc
     8341 tgatgtgccc gtggctgctt gttagtgacg ggaaaatcac gctgaagctg cacattcggc
     8401 cgggtgcaaa gcagacggcg gttacaggat tgcacggcga agcgctcaag atacgtctcg
     8461 ctgcagctcc aatcgatggt caggccaacg cggctttgat cgcatttgtt gccgatcgcc
     8521 taggattgcc caagtcggcg gtctgcctga gaagcggtca gacctcgcgc cgcaaggtgc
     8581 ttgaagtgat cggggcaccg gcagatgccg cgcagcgctt actgccgatt agctgaacct
     8641 ggattcctgg atttcgaaaa cgatgcgtcc tcctacgagc gtcgtatgca cccggccggt
     8701 catttcgcta tccatgaacg gggaattctt tccctcgctg cgcaacgcca tgggcttgac
     8761 gcaccacgtc tcagtcgggt caaagacaca gatatccgca gggcacccgg cgctcagtga
     8821 gcccgcttca atgccaagaa tggcggcagg gtcgcaggtg accctggcga ttgccgttag
     8881 caggggaagt ttcatttcca ccgcccattt caagaccagc ggcagcagca gttcgagccc
     8941 ggtagcgccc gagagggatt cagcaaaggg tagttgcttg ccgtcttcgt caacgggagt
     9001 atggtcagag caaatcgtag ccagtccatc cgccgcggct gcccgtagcg catcgcggtc
     9061 gctttccgac cggagcggcg ggtcaaagcg ggcatggctg tcaaagtagc cgatatccgc
     9121 ttcgcagaga tgcaggtgat gaatggaaac gtcacaactg atgttcagac cattgtttct
     9181 tgcctgacga accaaagcga tgcccgcggc cgaggacagc ctggccagat gcaagcgaac
     9241 tccggttacc tgtgccagct gaagtgcggt gacgatagcg actgtctcgg cacaaaccgg
     9301 aataccggca aggccaagcc ttgaggcaac ttcgccgtcg tgtgcaacgc cgtcgcgtgc
     9361 caagtagtga tcctgaggct gaaggcgggt cgcgtaaccg aaggtggcgg catattgcat
     9421 tgctcgtaac aacacctgcg tatcgaagat gggctgcttc gcttgcgaga aggcgatgca
     9481 gccggcgcgc gtcaggctga ccatttcgga gagcttttcg ccagccagtt gctgggtgag
     9541 tgcgccaata ggaaaaaccc gcgccagacc cagtgcttca ctgcgccgga ccaggcgctc
     9601 aaccagtcca ggctcgtcga gcggaggttt ggtgtcgggc ggacaggcca ctgccgtgac
     9661 gccacccgca actgccgctg aaagttcgga ttcgaggttg gccagacgcg tagacaggtc
     9721 aaccagcccg ggacagacta ttcgtccgct ggcatcaagt acctgcgccg gcttaaaacc
     9781 tgatggcggc tggccgatgg cctggatgcg cccgtcggcg acaaatacgt ctgcttgctc
     9841 atcgatgccg ttctttgggt cgatcactcg gccgtttttg atttgaatat gggggagttc
     9901 gaatctgctg aattcgctgt gcatcgttca gttccccgcc agaatgctca tcactgccat
     9961 gcgcaccgca atgccgaagg taacctgcgg caagatgaca gcctgtggcc cgtcggcgac
    10021 ctctgaatcg atttcaacgc cacgattcat cggacccgga tgcatcacga tggcgtcgtc
    10081 tttggccagt gccagcgttt caggcgtcag tccgtaactg ctgaagtatt cgcgtgccga
    10141 gggcagcaat accccattca tgcgttcgtt ttgcaggcgt agcattatta cgacgtcgca
    10201 atccctgagt ccttcctgca ggtcgttgca gacgcgcaca cccatctttt caacgtccgc
    10261 tggcatcaag gtctgtgggc cgaccgcgcg aatctgggcg actcccaggg ttttcagcgc
    10321 gtgaatgtcc gagcgtgcga cacgagaatg aaggatgtcg ccaacgatgg caacggtcag
    10381 attggtgaaa ccctttttgt agtgacggat cgtgtacatg tcgagcagac cctgtgtcgg
    10441 gtgcgaatgt cgtccatcac cggcattgac gacatgcaca tcagttcgac caactcggcg
    10501 caagtgatca acgataaggt aaggcgcacc ggaagcggcg tgacggacaa caaacatgtc
    10561 ggcgtgcatg gcaaccagat tgtcgacggt atcgagcaag gtctcaccct tggccgttga
    10621 tgagcgagtc acatcaaggt tgatgacgtc ggccgagagg cgcttggccg cgatttcgaa
    10681 cgtagtgcgc gtgcgtgtgg aattctcgaa aaacacgttg aaaacgcttt ttccccgaag
    10741 cagcggtacc ttcttcacgt cacggtcgga gactttaagg aagctggacg ccgtatcgag
    10801 gatttgcgtc aatatttcaa ctggcagacc atgaatcgat aacaggtgcg tcagttcccc
    10861 gttggtgttg agttgcggat tcttgtgcag agaactaagc atgttcaacc cgcttccatt
    10921 gcagcctccc cgtcgcttcg ggttcgagca caagctcttc gttctcctcc agatcaactt
    10981 gccaggtgca gaattcggca cagatcggca actcgcgtcc cccacgatcg gcaagaacgg
    11041 caagcttgat gctggcggga cgtccgtagt cgaataactc gtttaccgcc gcacgtgttg
    11101 tgcggccggt gtatagaacg tcgtcaacca gaatcagatg aagtgcctcg acgtcgaagg
    11161 gaatcgacgt gggcttgacc tgcggatgca accctttctc gccaaaatca tcgcggtaga
    11221 acgaaacgtc gatctgcccg atttctccgg ctaatccgag taattcgtgc agacgtcggg
    11281 cgacccagac accaccgctg tgaatcccca ccagcatggt gtttgccttc agttgcgtgc
    11341 ggatgagcgc tgccagttcg gcgcattgcg cttcggcatc aggcaacttg gtgagtggca
    11401 tatattttgt gatccgtagg gtcatcgaca ggttgtttag cgctgcgttc aaaaaacccc
    11461 tgcaaaacga tctgggcggc aagagcatct atatgttctt tggcgctttt cgcattatga
    11521 ccgctttcgc gcagcctttc ttccgcttcg actgaactga gccgttcttc ggcgtaatcg
    11581 acggcaatgc cgaagcggcc gcgtaactga ttggcaaagc gagtgcagcg cgcggtcatg
    11641 gcgtgcgtcg tgccgtcgag tgctaccggg aggccgacaa taagtctgac gggttgccat
    11701 tcgtcgatca gcgcggcgat cgcggcgaag cgttcgacgt taacctcgct ccgaatcgtt
    11761 gtcagtggat gtgcctgacc aagcaagcac tcgccgacgg ctacgccaat ccgtttttcg
    11821 ccgaagtcaa aagcgagcac cgttccgcgg gtactcgtgg tcgcgtcagg catgtccggc
    11881 cttgacggcg agattcttga aatcgatgcc cagtatttcc atcgccgacg gcaatctgtc
    11941 ttcgtagggg agttcgaaca gaatgtgggg gtcggccggc actgtcagcc aggcattctg
    12001 agccagctcg tgttcgatct ggccagctcc ccagcctgaa tagccgagcg ttaccatgat
    12061 ttcgtgcggt tgtccgtcac gtgcaacagc ctgcaggacg tcgcgcgagc tggttagtcc
    12121 aacgtcctga ttgacggcca gcgttgactg ccagtcgccg accggtcgat gcaggacaaa
    12181 accccgatca gtctgaaccg gcccaccgaa gaagatcggc aaattagcca tgcccagagt
    12241 ctcgaaaggc acatcgattt tttcgaacag ggtcgccagg ctcatgtcga tcgggcgatt
    12301 gacgatgatt ccgagtgcgc cctgatcgtt gtgttcggca atgtaaatca gggatttaga
    12361 aaaatacgga tcgtccatcg ccggcatggc gataaggaaa tgatcggtca ggtcaaagct
    12421 ttgcataggt acattttaat cattggcgcc agtcgataca aagggaatca atgcctggtt
    12481 tggatcgagg ctggtccgga cgcgatctgc atgccaccgc tcacctaacc gtgggcagta
    12541 tccggcggct gaaaaacccg atccatgacg ctctgtggca catgggcatt gccggacgat
    12601 ccgcttgccg aagaatcatc gattcgacca cactcaattg gctcgtcagt cccgacaagc
    12661 tctggggagc gcttggcagt ggtcaggcgt ggcgttgctg cgaaacgaaa tccgctacca
    12721 gctgcagcaa ttcgtcttcc tgatacggct tgccgaggta gttattgaca ccgatttcaa
    12781 aggcataggc acggtgcttc tcggctgtac gcgaagtgat catgatgatc ggaattgcct
    12841 taaggcggct gtctgcacgg atgtttcgcg tgaggtcgaa accgtccatg cgcggcattt
    12901 cgatatcaac gatcatcacc tctggaacaa catcgagcaa ttgttcaaga gcctcgacgc
    12961 catccttggc agtgagtact tgataacctt cacgcgccag caaacggccg gtgatcttgc
    13021 gcacagtcaa ggagtcatca acgatcatga ccttgggcag cgtggcggta gccgtctctt
    13081 cggcagggag ttcgggcgtt gtcgaaatcg gggcggccaa ttcaacaacc gatcgacggc
    13141 ttagcgccac cggatttagt atcaacacga cctggccgtt gccaagaacc gtcgccccat
    13201 cgataccgat gacgcgggca agttgcggtc cgatattctt gacaacgatt tcgtggttgc
    13261 cgagaagctc atcgacctgt accgcaatcc gttgcgcacc gctacgcagc agcaagaccc
    13321 agtactggcg ttgatgatcg ggatgggtct gtatttcacc gagcatatgc ggcaggtaat
    13381 tgaatgggta ttggttcccc atccattcgg cgctaccggc atcacgaatt cggctcaggg
    13441 cgtcttcctt gagatcgagc acctgctcga tcatcactga tggaatggca tagttcttgt
    13501 tgccggcgcg taccaacaag gccttggtaa cagccaacgt cagcggaatg taaagcagga
    13561 actgcgttcc ttggccggac gcggatacta tttcgatccg accgcccagg cggctaacct
    13621 cggttttgac cacatccatg ccaacaccgc gacccgcgat ctgcgatacg tcggtagcag
    13681 tcgaaaagcc cgaagtgaag atcatatctg caaggcgcgt cgggctggct tcttcgtcgg
    13741 cagtcagcag cccggcgtca atggcacgcg cacgaattcg ctcatagtcg agtccggcac
    13801 catcatcggt aaggctcaga atgatttcgt taccttcctg tttgagcgcc agtgaaatct
    13861 cgccgatacc cggcttgccc tgcgcaaccc gtatttcgcg ttcttccagg ccgtgagcga
    13921 tagcgttgcg aagcaagtgc tccagcggag cgcccatctt gtccagtacg cttcggtcaa
    13981 gttctacctg gcttcccact atttcaaggt tggcgcgttt gttaagctcc ttggaggttt
    14041 gccggacaat gcggtacagg cgatccgcca gactgccaaa tggcaccatg cgaactgcca
    14101 taagggcttg ttgaacctca cggttcaggc gagcctgggc gatgattgag gcattggcgt
    14161 cgtcaaggtt cttcagcagg ttttgctgga cagtggcaac gtcgttgacc gactccgcca
    14221 tcatccgtgt aagttcctgg aagcgggtaa aacggtccag ttccagcgga tcgaacccgg
    14281 cgtgctgctc attggtttgc gcagtgtgcg attgcatttg cgattcagcc tgaatttcga
    14341 cttcacgcaa ctgacggcgc aaacgaatca cgttttccgt cagatcgagc agagacgttt
    14401 tcaggctgcg catctcgcct tcaatccgcg cgcgggcgat cgacaattca cccgcttcat
    14461 ttaccagtcg gtcgataacg tctgcgcgaa cgcgcaaggt ggcccgttgt gcgagagctt
    14521 cggcatcgac ttcggcctcg gcaacgcgca atcgatcgcc acgacgatcc gtccggcgct
    14581 ctacagtaac agcaactttc tccggcggct ccgcacccgg cttcgcaagc gcagcgtcgt
    14641 cgaccggcac atgcggtgca gcaggcgttt caggcagttg gtcgagtttt tctccttttt
    14701 ggagacgttc gataatgtgt agcaccgaat caaaggcgct ttcgatttcg tcaatcactt
    14761 cgagcgggac gccatcggcg ctgctggcct gatcaacgcg tgtttcaatg gcgtgtgtta
    14821 tttcgcccag attcattgcg ccagccatcc gggcgctgcc cttgagggta tgcaactggc
    14881 gtttgagcgt tcgaaacgct tccgcactgt ttggctcggt acgcaaggca cgcagctgag
    14941 tggcaatacc ctgattcaga tcgaccgctt cttcaaggaa tataggcagc agttgctcgt
    15001 cgatctcgtc cttcaatagc ttgatttcag acaagaaaga aggtttgtgt tcgaattcgg
    15061 ctttggcggt ttcctgcggt ggcgctgttt cggcgctcgc tagctgttcc gttgcggctt
    15121 catgctctgc aggcggttcc tgagatgtaa ccaggtttgc cggataaagt gaatccaggg
    15181 cttcgatcag ccctgcgggc acgtcgggtt cgcgttcatc ggctagggat gacagcatca
    15241 gatcaagctc gccgacggcc tgtctgacta tcccgagggc ttccaggctg cctggttgtg
    15301 ccgaattgtc gcggcgcaac agtgcatgtt ccagcgcaag gcctaattga ttgacggacg
    15361 aaatcccgac tgttgcggag atgccggcga gtgtgtgagc cgcccggtac atatcctgcg
    15421 gggtaggcgt caattcatcc ttttccagga ctggcagttc gcggtgcaac gtagccagat
    15481 gaacgtctgc ttcttcagaa aatatctcat atagcgttgg cgagatggag attcgcaatt
    15541 gtgcttccga ctcgctcgct acaggctcga cagctggagg ttcgacatcc ggcagcgcgc
    15601 cctcctcagc ttgcgccgcc gcttcctcga cgggagtgtc aaagctgaaa tccgattcat
    15661 cgatttcctc aactatctcg agatcgctgg cgagattggc cccctcttgt tcaggcggtg
    15721 gttgcggggc atcgaattca atcaattcgg cgctctgaag agcctctggt gattcttccg
    15781 attccagggg agtttccgac ggcagttcgt caagcgagat cgtttcgagt tcaccctcga
    15841 cttcgctact cgccgttgcg tcaacagtcg gcgccggagg agcctccgga acttcttcct
    15901 tctctcgtcg aagcgaatcg gaaaaagtga tcaggtcggt cggatcggga gcacttcctg
    15961 tttgggtttc cagatagcgg acccatgtcg cgaaaacgct gtgtgcctgc tcaagcagat
    16021 cgaaaatgga agcgccaact tccagctcct gccgtagcca tagattgagg gtctgctcaa
    16081 tggaccaggc ggcctcgcca acatccgtca ggccaaccat gcgtccactg cccttgagcg
    16141 tgtggaacga acgccgaatc gttgtcaata attcgacgtt atgtggttgg cctttcagcg
    16201 cctgcaggtt ctcatcaatg gtgccaagaa cctcgttcgc ttcttccaaa aatatatcaa
    16261 gcagttcggc gtcgatctct tcgttgctgg cttgcgagag ctgaaccgtt tctgccgatg
    16321 atttcatcga ttcagggagt tccggcttga gtgttgccat gaactggtca atctgcggcg
    16381 aagcaactcc ccccgccttg aatgccgtca gcatggcctt ggcctgttcg ccgagtgccg
    16441 tgtcagcgac gagatcggca tcgttcttca gtgccgacag attgtgtctt acctgttcga
    16501 tcagattctc gttgcccgcc tgctccttga gagcgccgag aagcgcgtgt gtctcaagct
    16561 tgagtcgctc gacctcctgc tcaaccgaaa tgtcggattt gggtacttcc gcatccccgg
    16621 ccatttcgaa tgtcccgatc tcttcgaacg gtccggactc atcaattcct tcaaattcgt
    16681 cgagctcgcc ggtctcttca gcctccagcg ccgtgcctga ctgcatctgc ctgacgaagc
    16741 cttcaaagtc gacagcgcca tgttgcaggg aatcaataaa gaagccgatc atggacaact
    16801 ggttggccac gcgctcgaaa tccgcttcct gtgaggggct ctcaggttct gcaaagcgtt
    16861 tgatttcttc cgaacactcg cgcacagcaa cggcagcagc gtcgtggcgc atcatggtca
    16921 aggcgcctac gacctggcgg aacggaacct ccaggctgac gagatccgcg ctcttcgagg
    16981 tatcgcggaa gaatccatcg agcacctgtt cgatttgtgc aaaattgctt tggatctcct
    17041 tggcgacctg cccgaccagc agcttttcct gagccttgcg cgacatctcg tcgagcaacg
    17101 ggacttccga tccgggctgt ggcggagttc cagcaatgca accatgtatc cgggctacgg
    17161 tgacatcgac ctggtgagca aaatcgtttc cgagctgctc aaaattttcc tgcgcattct
    17221 gggtcaggag aatcgcggtt gcgatttcca tcgccagcga ttccgaatgc cgggagctgc
    17281 cttctgacag ccagttggcg gctgcggcaa tggcttgcgc cagccggcgg aagtcagtat
    17341 ggccaagctg ttcgacgact gcagacaagg cgttggcgtg ttccctgaac aagggaagcg
    17401 attgcatggt accggtgcag tacttattcc aggcttcttc ggtcagcgtg atcacatcac
    17461 gcagtttgcg gcgaacggct tcctccggtg ccggggcact cgtggcggtg gtcgggatga
    17521 ttgcatgcaa ctgataggtc ttcttgacca gccgaaccgt atcgttgttg ctgtcagcgc
    17581 tggcgacgaa atagagcacg tcgcgcatca ggcgctcagc gacattcttc gatccttcaa
    17641 tcaagcgacg tatctgcaga tcaatgcgcg cgcacaattg cttgacgttc gtctctcccg
    17701 gcaagctgtc ctcggccaat gctgagagaa agccactcgt aatccaccag aaagcgcgag
    17761 ccgacgggac atcctgcgtg ttttcaatac gctttatcgc acccagcatt tcgctgatgc
    17821 cacggctatc gtgtggcgcg cgcagccatg ccagaagacc gtgttgaaag cgcgcgcgtt
    17881 cttgtcgcgc aaggcgctgc aagtcactgt gcgcgatctt ccgcgccggg gtttcgcggc
    17941 gcggcaggcg ggcggtcaag tccgggaaga agagatcagt ggccgagacg tgctccagcc
    18001 ctcgcgcctc ctgcacctcg cggtagagcg gcaaaaggcg caatggctgg ttcggctggc
    18061 cgctgatcag gtcaccgaga tagtggctga tggcagccag ggagcgctgt gcaagagcga
    18121 cgaaatcgtc gtcggcagga cgtttctgtt gctcaatccc ctcaagcaac gattcgaccg
    18181 cctctgcaaa ctgagtgacc ccgtcaaggc cgacgatggt cagcgcacct tgtacctggt
    18241 gcaagtgtgt gcggctgaat ctaagctgcg tcaggtctcc tgaacctgcg acaacgcttg
    18301 cagagaattg ctgcagcgcc tggtcagcac gttccagtgc aaggtcgatc tcgctcttga
    18361 cccaggtcag cgggccgaca tcgaattccg ttgcggcatt cattggggca gtgtctccga
    18421 tctctgctca ggctaatcag cccaccttga agccggcaac cgaacccttc agttccgttg
    18481 ccaggtctgc cagttcgccg accgcagtgg cagtttgttg cgttccggcc gtcgtctgct
    18541 ccgtaacgcg cagaatgtct tgcatttttc ccgccacgct ggttgccgaa tcggcttgtt
    18601 ggcgcgttgc tcctgaaatg ttctcgatca gttcggcaag attttgtgaa acgctcccga
    18661 tctcggcaag cgcctggccc gttgcatcgg atagtctcgc gccttcaacc acgccgtgcg
    18721 ttgattcttc catggcggac acagcgtcct gcgtgtcggt ctgaatggtt ttcacaatcg
    18781 ccgcaatctg cttcgtcgct tcgccggaac gttcggcgag acgctgcact tcctcggcaa
    18841 ccacggtgaa ccctcgcccg gcatcgccgg cagaagcggc ctgaatggcg gcgttaagcg
    18901 ccagtacgtt ggtttgttcg gtaatgtcgg aaatcagctc aacgatttca ccgatttcct
    18961 gtgaagattc gccaagccgc ttgatccgtt ttgatgtttc ttgaatctgt tcgcgaattt
    19021 cggtcatccc cttgattgaa tcctgcaccg cttgcgtgcc ttttccggca gcggccaacg
    19081 actgacgcgc tacctgggcc gactggtcgg cgtcacccga cactttggtc atcgaactgg
    19141 ccatggccag gacggaaaga ccggccgcct gaatttcttc tgactgacgt tgcgctgcct
    19201 caagtaattc gacggaggtt tgctgtgcga tgccagtggc tgttgtcact cgccccgccg
    19261 catcgttgat gcggcgtacc agtacgcgta gttcctcaat ggtgtagttg atcgagtcgg
    19321 caatggcacc ggtaatgtcc tcggaaacgg tcgcggtaac ggtcaggtcg ccgtcagcca
    19381 aatcccccag ttcgttcatc aggcgcagaa tggcatcctg attctggcga ttgatcgact
    19441 cagtggcctg tcggctctcg ttggctgttt ccgcctggcg ccgagagtcg tcgaggtaaa
    19501 ccttggccat catggccagc gtcgccaatg ccagaaacat cagcacggca aatgttacgc
    19561 cgtatgccag ccgcccgccc tgctggtagg cgtttgccag gttatccgtt gactcgagca
    19621 agccttcgct gttgcggaaa attgaagaac ccgcccgctt cgccagtgtc agtcgttgca
    19681 tgctgccgag aatgccaccg acagcgtcct ggtactcctt gaaactggtt tccaggtcgc
    19741 cgagtttgcg cttggtgtct gcgtctgttg tgccagaaat gcgcaggttt tcgttaccct
    19801 tttgcagggc gttcagcaac tcgcggaagg tgttcgtatc cttgcccagc aggaaggcga
    19861 tttcggggct gatttcgtcg cccaccaaca acgaactggc gttcttcgca atacgttgcg
    19921 tcaacatcac catctggttg gccgcggcaa tctcccgcac acctgcaccg gactgtagct
    19981 tgagtgcagc aacctgctcc gaaagctcga gcatttccgg gttcttgttc gtaatggaag
    20041 ccacgtcctt gcttagtgcg accaggttgg tttccatatc aagcaactgg accgcgtcct
    20101 tgtccgttgc actccaggtt ttggttagcg cctgcagttg gggttgcacg tcatcgggcg
    20161 atggcggcac cgtcgtgccg gccagttcgc cgcctttggt caggcgttcc agattggtga
    20221 caaactggct gtgcgaatca cgcaactgct tgaatgccga cgtatttccc tgcaatgcaa
    20281 ggctggaagc cttggccaga cgctgcgaga gcattcgcat ttcaccggcg gtcgcaatgt
    20341 aggtactgtt gaaagccgat tcgcggttgt cgcgatacac gaaagcgatg atgatcacca
    20401 cgataaggag aaaagccccg ccgagaatct gcagctgctt gatgacggag tactgaccca
    20461 gaaagcccgg accggaggcg cttttctttg tggcgatcgg ttgagcatca tccatgatcg
    20521 tggtgtcgga tgcggtttga cccgcagcat catttgagcc agatagtttc aggttgaacg
    20581 ccatgttgga tctccggtag caacgtattg agcgtacgtg tcttcaggta cggttgaaat
    20641 acatgagggt ctcaaacgcc aatgtccatg aaagagtcat ccgaaaggag ccggcgcaca
    20701 ttcagcatat tccagcggcg cccctcgtta tcggaataag cttcactcga ccagggaggc
    20761 gcgtcgggat cagtagccac cggtgtcaga tcgtcaagat tgcgcaggcc gagaacgcgt
    20821 ggaatcagca gtgcggcgtt gtcaccatgg cgagtgccaa tcagcagcaa tcgcgaactg
    20881 gcgttttgtg gagtggcttc cttgccctga aaagcggaaa aatcggcgac cgcgtaaaga
    20941 ttcccgcgaa tattcgcaat gccggcaaac cagggcttgg tcaaaggaac gccggtcagt
    21001 ggcgtcagcg gaatgatttc accggaatcg aacaggctga tcagccagaa gtcggatcct
    21061 gcctgaacgc ccagcaggcc cgctgcactc tggtcgctga ctccggcaag ccgcttggcc
    21121 aagtgcgcct gaaactcgcg tagactccct ttttttgcca taccgtgtct tgatgcctac
    21181 agggctgcga ttttggccag cagctcttca ctattgaccg gtttgaccag gtagtcctgc
    21241 gcgccctggc gcaagcccca gatcttgtcg gtttcctggc cctttgaggt gcacacaatg
    21301 acagggatat gcttggtcgc ttcgtcgcgt gtcagcgttc gggtcgcctg ataaccattg
    21361 agtccgggca tcacgacatc catcaagacc agatccggca attcggcctt tgcttttgcg
    21421 atgccctctt cgccgctttc ggcagtaatg acttggtagc catgcttgga caaaagctca
    21481 agcatggcat gacgttcggt aggtgaatcg tcaacgacga ggattttctt gattgacatg
    21541 gcggtgactc cctgggtgcc cttgaagtta tgaattcaat ctcagtccgg ctgacgcgca
    21601 aaagttgcga ctgcctgcag cagactgtct ttggtaaacg gtttggttaa gtactggtcg
    21661 gaaccgacca tacgtccgcg cgcgcgatcg aacagaccgt cctttgacga aagcatgatc
    21721 agcggtgtat tgcggaagcg ctggttcttt ttgatcagcg cacaggtctg gtagccatcg
    21781 agccggggca tcatgatgtc gcagaagatg acttcgggct ggtggtcagc gatttttgcg
    21841 agagcgtcaa aaccatcctc ggcgagaaca acctgacaac cggcctgcac gagaaatatt
    21901 tccgcgctac gccggatagt gttgctgtcg tcgatgatca tcactttgat gccactcagg
    21961 ttcgcagcgt cggccaattt tttcctccag gctgcccttc atttctaatc ttgagggctc
    22021 tgatcaaatc ggatggtatg gctaatacat ctcttatatt caggcggtac tatacatcaa
    22081 tgatttagat ttcaagcatt tcgaattcat ccttgcgcgc accgcattcc gggcacaccc
    22141 agttgatcgg gatatcccgc cagcgtgttc ccgccggaat gccttcttcc ggactgcctg
    22201 ccgcttcgtc atatacgtag ccgcagatca ggcacatcca agtgcaatat gcggcatctt
    22261 cagtagtttt catagcgtga ttcgtatttc gggtaaaatc ggccgcgtga attgcctccg
    22321 attctagcca tacgttcttc cctctgccaa ggcttttctt gtcccggcgc cggttcgctt
    22381 tcatggattc attatcgatg caactgccgc ctccgattgt cctgacattt gccgccagcg
    22441 accccacggg aggtgccggc attcaggccg acctgatgac ggttacatcg ctggcttgcc
    22501 atgcgctatc tgtcataacg gcgttgacgg tgcaggacac tctcggcatc acgagcgttc
    22561 tgcccgtaaa cgccgactgg gtcgaagctc aggcgcgcac ccttctttca gacatgccgg
    22621 tcgcttgctt caagataggc gttcttggaa gtgtcgaaaa catcagggcg gttgctcgaa
    22681 tactgtccga tcatcccgat cttccggtgg tgtttgatcc ggtgcttgca tccgggcgag
    22741 gagatcaact ggctgatgag agcatggttt tggcattaac cgaattgctg ctgccgctgg
    22801 tcagcatcct gacaccgaat tcccttgagg cgcgctggtt ggccggcatg acaaatgcgg
    22861 aggactgcga aagcgatatg cggctcgaag aatgcgccca ccgactgatc gacc
//
LOCUS       DAORPM010000006        21563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_408_length_21563_cov_15.388367, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000006 DAORPM010000000
VERSION     DAORPM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 21563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21563
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_408_length_21563_cov_15.388367"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLD53_00600"
     CDS             <1..657
                     /locus_tag="PLD53_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HRC59431.1"
                     /translation="GGPEIEAMRGVWAELQNHALELAGQEERVDHRSLEVQREEALSH
                     GDNMRAEELDREPELKLGPAANAIERREQFAAEAEGRDYAPLTQRGARVHAVRQAKEM
                     FAEMRERLELARDAWAEAREEGHGHVSAGLAALRAVAGRQAKDLDQDEIKQRLARIAG
                     RDLGEDGTGHEAGADAIRDRLKQVLGRGKLDPRLERQRNLEEEREKQRESDRDLGWEL
                     "
     gene            693..920
                     /locus_tag="PLD53_00605"
                     /pseudo
     CDS             693..920
                     /locus_tag="PLD53_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1051..1338)
                     /locus_tag="PLD53_00610"
     CDS             complement(1051..1338)
                     /locus_tag="PLD53_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRC59432.1"
                     /translation="MKVLAFSPAAEADIDGIWDYSADNWGPEQADRYTDEIRDACHAL
                     ASGRKQGRVVDVRPRYLKISTGSHVIYYRDQGDRLEIVRVLHSRMDSNRHL"
     gene            complement(1335..1583)
                     /locus_tag="PLD53_00615"
     CDS             complement(1335..1583)
                     /locus_tag="PLD53_00615"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRC59433.1"
                     /translation="MPRNTSVTIGDHFASFISEQVQRGRYGSASDVVRAGLRLLEEHE
                     GKVKALEAALIEGEESGEPRPFDFDAFKARKRAEYAAR"
     gene            2134..2439
                     /locus_tag="PLD53_00620"
     CDS             2134..2439
                     /locus_tag="PLD53_00620"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HRC59434.1"
                     /translation="MEAFFAATGAQIDVTEEPRAYYNITTDRIHMPPIETFHRVTGYY
                     GTLAHEVATPPLSLPIMIVRISVPLIVIIMGAMPAVGRLPKFTERRVSADESMVQDR"
     gene            complement(2493..2819)
                     /locus_tag="PLD53_00625"
     CDS             complement(2493..2819)
                     /locus_tag="PLD53_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59435.1"
                     /translation="MLFFIKAMRKFLGRAVMPSESQARGPEALCALEENWQTELSAFE
                     ASMDAKHLQAAGDSLFQAINRIYGELAAVVAFQAGGWPRPWKRSYSYPPANVTRPGTI
                     QFCLRC"
     gene            3269..3820
                     /locus_tag="PLD53_00630"
     CDS             3269..3820
                     /locus_tag="PLD53_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59436.1"
                     /translation="MLLPSRTGRAIARLFCDVRGGSDQDCEQYLFETILPVFQLALQN
                     APSIKLGTFCVLLSVAQNAERFGARGDPSGIISEELGLSNLPKHMENLSSGSDARPGL
                     ELVELQRHPLNRRISLPKLTEKGLRLVANIAAALQHKPPSAVRFPKEEKLRDAPSPDD
                     VKAFSDDDFDFDEIEWLRPDDDT"
     gene            4424..5308
                     /locus_tag="PLD53_00635"
     CDS             4424..5308
                     /locus_tag="PLD53_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC59437.1"
                     /translation="MGAMMPETQSTTGTGRWLLTGASGRIGRMLMRHWQEAPPPADLV
                     LQSRREGGGLLWDPLLQPLPATAGRFSCLIAFAGVTPAAGAALDANLALAEATLAAAH
                     AAGIARVLLTSSSAVYGAPQGDAPFHESDALRPLNPYGAAKVAMEAACAPWRARGLEI
                     CILRIGNVTGADVLLLNGARATAAAPLRIDRFADGQGPRRSYVGPADLARVVESLAAA
                     EGPLPPVLNIGAPQPVDMADLADAAGMPWIWTAAPLQAVQNITLDCRALDSLHPANRT
                     ASSPVEMVRQWTRLKDPT"
     gene            5305..5910
                     /locus_tag="PLD53_00640"
     CDS             5305..5910
                     /locus_tag="PLD53_00640"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRC59438.1"
                     /translation="MTPAKRIFDILVAVVLGIVLIIPVLVTALLILIRDGRPVFYISE
                     RMKSPTEGFNLVKFRTMKVVASDSGVSGGDKSARITRTGAFLRRSRLDEVPQLWNVLK
                     GDISFVGPRPPLRQYVERFPEIYREVLKSRPGITGLASVYFHAHEERLLARARTTAET
                     DAIYSRACVPRKARIDLIYQRRRNICVDAGIMLKTVFKRLK"
     gene            5936..6925
                     /locus_tag="PLD53_00645"
     CDS             5936..6925
                     /locus_tag="PLD53_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC59439.1"
                     /translation="MNWLITGGSGFIGSNLCQSLARDGESFRIIDKRPSPFFPGKVIE
                     MDILDQEQLVQSVSGDTIVHLAAEHRDDIRPRDRYDQVNVEGTRNICRAASLRGIDRI
                     VFTSSVAVYGFAAPDTGEDGAIDPFNDYGRTKFEAEEVLRAWQAEAPERRSLVIIRPT
                     VVFGPGNRGNVFNLISQIARGRFVMVGVGENRKSMAYVENVVAFIRHMAKSGPGVHLS
                     NYIDKPDLSMNELVSHVRATVLGKPGVGLRLPSWLGMGLGHVADGVTRITGKRLPLSA
                     IRVRKFTTSTAFATRAHQVPGFVAPVSLADGMARTLDAEFLHPEATRPIFYTE"
     gene            complement(6959..8221)
                     /locus_tag="PLD53_00650"
     CDS             complement(6959..8221)
                     /locus_tag="PLD53_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC59440.1"
                     /translation="MSAAEQDGKKRLSIVFVSQYFFPEQFSNNAIVEELVRRGHDVDV
                     VTGVPNYGRDGFFEGYSNSEAREEDWRGARIHRARSVARGKSKLRLLLNYITFPVTGS
                     WTALRKVKRRPDVSFVSMPSPPFQALPAIVLRWMRGVPVVYWVQDIWPETAIDMLRLK
                     NPIIVKALSAVCGWIFRRADLVLVQSAAFPPMVARFGIVDERIRVLPNTAPDMYRPMA
                     PEEAPDAQLLMPAGGFRVMFAGNIGESQDFDTLLATADLLRDRGALHWVIIGSGRDLE
                     RVRARAAELGITDRFHLLGRHPEETMPGFFAHADALIVSLRDSDIFNRIVPYKVQCYM
                     ACGRPILAAIGGEGARIIETAQAGFAAPPSDPRAMAEAVGRMMDLTAAERQAMGGNAR
                     RYYEAYYSGAQVYGDLESWLYEAAALRR"
     gene            complement(8218..9387)
                     /gene="wecB"
                     /locus_tag="PLD53_00655"
     CDS             complement(8218..9387)
                     /gene="wecB"
                     /locus_tag="PLD53_00655"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRC59441.1"
                     /translation="MAKLKVMTILGTRPEIIRLSRVMARLDAFTDHRIVHTGQNWDYE
                     LNEVFFEDLGVRQPDHFLGTGGGSLGETLGKILIETETVIRAEKPDAVLVLGDTNSAI
                     SAIIARRLKVPVYHMEAGNRSFDRNVPEETNRKLVDHISDFNLVYTEHARRHLLSEGI
                     QHRRIYLTGSPMREVLEHYRDRIEASDVLSRLSLKPQGYFIVSLHREENVDNGERLAS
                     LRDTLNALAADYDMPVIVSTHPRTRKRLEALAAGAAGTNAIDQRVQYMVPFGFHDYNA
                     LQMQAFCAISDSGTIAEESSILQFPAITPRDAIERPEGLDVGCIIVTGLNRDTIREAV
                     AAATQLHAERKAAGIRHPLPADYAITNTSERVLSLILGTARLSNGWDGIRMNDLT"
     gene            complement(9390..10520)
                     /locus_tag="PLD53_00660"
     CDS             complement(9390..10520)
                     /locus_tag="PLD53_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRC59442.1"
                     /translation="MSRRIIVTGAGGLLGWHSASRLHAANCAARFRKEAVPYDIVALN
                     HGTFEDDAQLVAAVSGADAILHFAGVNRGTEAEVRDGNPQIAGSLVDACRQAGARPHV
                     TYANSTHAKLDTVYGKSKRLAGELLAGIGGGFTDLILPHIFGEGARPRYNNVTATFIE
                     AVINGESPQIDPNGRVQLLHAGAAAQIAIDAALARHDGELAPPPRPTGVPWLFETLQR
                     FHADYAANLFPDLADPFLRDLFNSYRAALYPAGFPRSLKVNADPRGTLFEAAKGGGGG
                     QTFLSWTEPGVTRGDHFHLGKVERFLVLEGEAMIRIRKVLGGPVWEYQVSGKTPAPVD
                     MPTLHTHSIQNIGDRPLLTLFWTNDLFDPSAPDTYFDPVLQD"
     gene            10708..11148
                     /locus_tag="PLD53_00665"
     CDS             10708..11148
                     /locus_tag="PLD53_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HRC59443.1"
                     /translation="MPCYRSNLNKHPPHAVAALNEGKCKMSEYRDKTQEEIRFRILRL
                     IEQNPGMSQRELAAEVGISTGGIHYVIKALIEKGLVKLGRFAVAEDKRRYSYVLTPKG
                     LTEKAAITRVFLARKMEEYELLKHELKTLSEVVRRDENLGDVSP"
     gene            11145..12431
                     /locus_tag="PLD53_00670"
     CDS             11145..12431
                     /locus_tag="PLD53_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59444.1"
                     /translation="MMIGTRLFNVGLRSLTLGVRFLFIFFLARYLTPETVGYYGLFTA
                     TVGYCLYFVGLDFYTYLTREILKSPQDRRGGLLKAQAGLSASLFLVLIPVALIALPYA
                     GWPPALLWWFLPILLFEYLNQEIYRLLIALSRQIPASILLFMRQGSWAIATLLVMGMM
                     PEARNLHVLMAFWGISGLMTAMLGLLLIHHLRLGGWRDTIDWRWLWRGIFVSAAFLVA
                     TLALRGMQTLDRYWLESLGGIESVAAYVLFLGVAGTLLTFLDAGVFAFGYPELIHQHN
                     IGDTDATRTTLRKMLIQTLIISAGFGIASWLALPYLLDWIGKPVYEDRREIFPLLVLA
                     MIVNALGMIPHYALYASRRDRPIIHSHLASLAAFVLTTWLLALTLPQGKLELAIPLAL
                     NAGFLLILLWKTLALLFPRGADPASQSSQPTMANFK"
     gene            12428..13585
                     /gene="pseC"
                     /locus_tag="PLD53_00675"
     CDS             12428..13585
                     /gene="pseC"
                     /locus_tag="PLD53_00675"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HRC59445.1"
                     /translation="MIRYGQQDITQADIDAVVGVLKSVNLTQGPSIPAFEQAVTAETG
                     ARHAIAVNSATSALHIACIALDLGPGDVLWTTPNTFVASSNCALYCGGAVDFVDIDPR
                     TWNLCPDRLEEKLIAAEKTGRLPKIVVPVHLTGQPCDMARIHALGQKYGFRIIEDASH
                     AIGGQYRGEPIGNCRYSDITVFSFHPVKIITTAEGGMAMTNDDHLATRLGLLRSHGIT
                     RDAALMDGDSDGPWYYQQVDLGYNYRMTDMQAVLGVSQISRLHPYVRRRHEIAARYND
                     MLADLPVTLPWQHPDSYSAWHLYVIRLQLDRIEASHRQVFEALRSRDIMVNLHYIPVH
                     TQPWYRKMGFKDGDFPQAEQYYREAISIPMHPSLTDAEQDFVIDVLRDNLK"
     gene            13582..14427
                     /locus_tag="PLD53_00680"
     CDS             13582..14427
                     /locus_tag="PLD53_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC59446.1"
                     /translation="MKLALGTVQFGVDYGAFGTGRQVARPQVTEILDLALAAGVDLLD
                     TARAYGEAEAVIGAQPASEQFRIVTKCPPIAPEVDAADALLAQFEASCRALGVKKVAG
                     YLLHFAGDIARPGVVDVLESLVADGRIERFGVSIYDFAEAEEICARYPVTLVQLPANV
                     LVPWFRDRGLPETVEVHVRSAFLQGFLLSDPLGLSDRFVEWRSVLETFRHRAGALGLS
                     PHQAALAPLLHSPYVHRVVVGCDTPAQLTELISAAATGPADLGDYATVTPELTDPRNW
                     QRSRT"
     gene            14424..14993
                     /locus_tag="PLD53_00685"
     CDS             14424..14993
                     /locus_tag="PLD53_00685"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRC59447.1"
                     /translation="MSNVTTLQDDPRQHGDGGRIYLRKLVPDDVGEAYLGWFRDPIVT
                     EYLDSRNLSREDVVSYMTEGHASGLHAMYGIFVAGTDQHIGNVKVGPIAWKHGTGGLV
                     TFIGERDFWGKGIAREAVRIGTRLAFDIHGLRKLHDGVADGNIGSLKAYQAAGWIVEA
                     RMRGQHIVNDNVRDRIVISAFNPRYFPPE"
     gene            15020..17050
                     /locus_tag="PLD53_00690"
     CDS             15020..17050
                     /locus_tag="PLD53_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC59448.1"
                     /translation="MKVTAIVQARMGSSRLPGKVMRKVGDHVLIDLLLTRLARARTVS
                     EIVLATTDQPSDDRLAKHVADLGYKVVRGSENDVLSRFVLAADATDGDILVRITGDCP
                     LVMPELVDTLVQGYQQADLDYLSNVDPPTWPDGLDVEVFSSTALRRAAAETSEQFDRE
                     HVTPFLRRGDFRRANVTNAQDLSKLRLTVDAAADLEVMESLINRVEDPVNLDLDALLA
                     LTNSEPELFDANSMLNRNEYAMTGRGQKLWMRARQIIPGGNMLLSKRAEMFLPEHWPA
                     YFDRTEGCVVWDLDGNSYLDLGLMAIGTNTLGYSHPDVDKAVMSVVAKGNMSTLNCPE
                     EVLLAERLIEISSPWGEMVRFARSGGEANAIAIRIARAASGRDGVAVCGYHGWHDWYL
                     SANLGDSENLAGHLLPGLNPAGVPRSLGASTFPFMYNDLARLEELIATKSIGVIKMEV
                     YRNIEPQDNFLQKVRDLATRNGIVLVFDECTSGFRETFGGLYKKYGVEPDMAVYGKTL
                     GNGYAITAVVGSRDVMEAAQSTFISSTFWTERIGPAAALATLDVMEREKSWEVITETG
                     RVWQRRLQQTADEAALPISISGLPSLSTYSVLGDDSAECKTYITQELLKRGILAGTGL
                     YASIAHQPAHLDKLFSELAVIFAKITDTRAAGKTVAALLDGPVCHTGFRRLN"
     gene            17061..18155
                     /gene="pseG"
                     /locus_tag="PLD53_00695"
     CDS             17061..18155
                     /gene="pseG"
                     /locus_tag="PLD53_00695"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HRC59449.1"
                     /translation="MSSVVIRTDAATMIGTGHVMRCLTLADELRDEGGAEVLFLCRPL
                     EGNLIALIAARGHRVMTLPALGSHVPSSTRPTDWLGTDQATDAADCQRALASQAIFDW
                     LIVDHYALDHEWETQMRPLCDRLLVVDDLADRSHDCDLLLDTGLGRTDADYRALIPDH
                     ALSLLGPRYALLRPEFRAHRAISLSRRDAPVLKQVLVTLGGTDPANMTCQALDALDAT
                     ALPLETEISVVMGASAPWLSDVRRRASTMKQSTRVAVDVKNMAEIMTDSDLAIGAAGG
                     TAWERCCLGLPSIQLVLAENQRGIGQSLQASGAAVSVVSPSEITDFLTELTARNELHS
                     WMKQSAAAAAAIVTGNGVSAVRSSMMAHNA"
     gene            18148..18672
                     /gene="pseH"
                     /locus_tag="PLD53_00700"
     CDS             18148..18672
                     /gene="pseH"
                     /locus_tag="PLD53_00700"
                     /EC_number="2.3.1.202"
                     /inference="COORDINATES: protein motif:HMM:TIGR03585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase"
                     /protein_id="HRC59450.1"
                     /translation="MLEQCSIREVERTDLPTLLSWRNHITVRSVMFNQNEISSDEHHN
                     WFVRVKEDSNRQLLIAEDESGLLGFAQFTGVARGGIAEWGFYARPDAPKGSGTKLCSL
                     ALDHAFESLELHKVYGQAIEKNAPSVAIHRKLGFTEEGVLRDHRHVNGAYVSLVCFGV
                     LNHEWATKRNRREV"
     gene            18669..19727
                     /gene="pseI"
                     /locus_tag="PLD53_00705"
     CDS             18669..19727
                     /gene="pseI"
                     /locus_tag="PLD53_00705"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HRC59451.1"
                     /translation="MNGITIDGRNIGPNQPPYVIAELSANHNGSLETALRIIDEAVIA
                     GADAVKLQTYRAETITLDSDSEEFVIRGGLWDGKRLHDLYTEAHMPWDWHQPLFDHAR
                     KRGITIFSSPFDSTAIDLLEDLNVPAYKIASFEAVDLPLIRYAASTGKPMIISTGMAD
                     AEEIGEAVAAAKDGGCRELAILHCVSGYPAPPGDYNLRTIPDMVERFGLVTGLSDHTL
                     DNTTAIASVVLGASIIEKHFTLNRNGGGPDDSFSLEPPEMKALCQGARTAWEALGRID
                     YGRKSSEQGNVQFRRSLYFVKDLKAGEVITPDSVRSVRPGYGLAPKYLEKILNAKVKQ
                     DVSKHSAVTWEKLKSPEE"
     gene            19745..20920
                     /locus_tag="PLD53_00710"
     CDS             19745..20920
                     /locus_tag="PLD53_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59452.1"
                     /translation="MIRISALLTLAAPTYIALTFTRFYVVNPQTQSIIGILLVATGCG
                     FMMASMSSFHIGRWVLASVAVIASAAAINTLVGNNFSIDSGVQLLANIGYAYAISKGP
                     MSVTAQKVITLLLSAFFMSHAVLGVNPEGIFTISRNFISVLMILSVAFYYIACTKNEK
                     SPEVLIPIFVLAICLWAIGRSGIISAALLLVGTTVLSNKKTTILAVYSLIAISVIAVA
                     GERLLAYVEIFQVGIERFERLSASGPDPRELIFDEYFAITSTHLKYIFFGTPLGLVSE
                     ISAVGGNPHNAYISLHASFGMLGLTYFMLLSAVSLAKLWRAKLRYLTLVFLILLLRSA
                     FDSTAFHGPLDVALFSCIFVALAKKRYDDMSSPPFSRIWTAMKLPNGRRQAKNEPWI"
     gene            21261..>21563
                     /locus_tag="PLD53_00715"
     CDS             21261..>21563
                     /locus_tag="PLD53_00715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HRC59453.1"
                     /translation="MNPRTLIGSDVWIGARAIVMEGVEIGSGAVVAAGSIVTKAVAPY
                     SIVAGVPAKHIKYRFPEEERQALLDGEWWNADEEQLKAKIAVFQKKITSVSDVLMLP"
BASE COUNT         4512 a         6377 c         6284 g         4390 t
ORIGIN      
        1 ggcgggccgg agatcgaagc catgcgcggg gtttgggccg agctgcaaaa ccatgctttg
       61 gaattggcgg gtcaggagga gcgtgtcgat caccggtcat tggaggtgca gcgcgaagag
      121 gcgctgtcgc atggcgacaa catgcgcgcc gaagagctgg accgtgagcc ggaactgaag
      181 cttggccccg ccgcaaatgc catcgagcgc cgcgagcaat tcgccgccga ggccgagggc
      241 cgcgattacg cgccgctgac ccagaggggc gcgcgggtac atgcggttcg ccaagctaag
      301 gagatgtttg cggaaatgcg cgagcggttg gagctggcgc gggatgcctg ggccgaggca
      361 cgggaggaag gccacggcca tgtcagcgca gggttggccg cgttgcgcgc cgttgcaggg
      421 cggcaggcga aagatctgga ccaggacgag atcaaacagc gcctggcacg gattgccggg
      481 cgtgatcttg gggaagatgg caccggccat gaagccgggg cggacgccat ccgcgaccgg
      541 ctcaagcagg tgcttgggcg cggcaagctc gatccgcgtc tggagcggca gcgcaatctg
      601 gaagaagagc gcgaaaagca gcgcgaaagc gacagggatc taggttggga gctatgagga
      661 tcctctgaat cgcgccgggt ttgccggagg ctgtccgcgt aaggatcgag gcggccgggg
      721 caacgcttcg gtttctccca ccctacagcc ctgatttcaa cccaatcgaa aaggccttct
      781 ccaagctcaa ggccctgttg cgcaaagcgt ccgagcgcac cgtttccggc ttgtgggatt
      841 gcattggcaa actcgtacat gtcttccaac cagaagagtg cgccaactat ttcagctcat
      901 gcggatacga tccagaatga tcggaaagcg ctctagcgta tatctgggtc cgttctatgt
      961 gccgcccccg aaacagaggt tggcatggat agcctccttt atcgttacat gtaactaagc
     1021 cgggggtcca tttcaaccca ccaccagccg ctagagatgc ctgtttgaat ccatgcggct
     1081 atgcagaacg cggacgattt cgagtcggtc gccctgatcc cgatagtaga tcacatgcga
     1141 tcctgtggaa attttcaggt atcggggcct cacgtcgacc actcggccct gtttgcgccc
     1201 cgaggccagc gcatggcagg cgtcccggat ttcatcggtg tagcggtctg cctgctctgg
     1261 tccccagttg tcagcgctat aatcccaaat accgtcaatg tcggcttctg ccgcaggcga
     1321 gaaggcaaga accttcatcg cgcggcatac tctgcccgtt tgcgggcctt gaacgcgtca
     1381 aagtcaaaag gacggggttc gccggattcc tcgccctcga tcagagctgc ttccaaagct
     1441 ttcaccttgc cctcgtgttc ttccagaagt cgcaatcctg cccgcaccac gtcgctggcc
     1501 gatccgtagc gtcctctctg cacctgctcg ctaatgaagc tggcaaagtg atccccgatc
     1561 gtaaccgacg tgttcctggg catcgctgcc gccctcccta ttctaagcaa aggtagatta
     1621 taccaaaaag tattacacgg caaccctggt ctgggggtta ggcaaacgtc ttgggcgggg
     1681 tcgagagcgc agctaaggag atgggtttcc attcatcgcc gccgcttcta tgccgatagg
     1741 tcaggattac gcgccccagc tctgccctga cattgatcga agcgacgacc tcaccatgcc
     1801 gagaccaagt gatgcgccgg tctgccccgg tgtcagcatg actttgcgct ttagccatcg
     1861 cacatcaatc gaccgatagc cttctgtcgt gtccttggac ccgaaatgct agcgccgccc
     1921 gcttccagta ccgcccggtg ttgaaaaggg gggtgatggg tgtgaagttg gcaagaaata
     1981 ggaacgtata cttcacactc cagaccggca gcgcccttcg cctacgccaa ggcctatcgt
     2041 gtcttcaatg ccgatcagat cgaaggcctg cccggggagt tctacatcct gcccgatccg
     2101 ccgcgtgatc ttggtaccaa ggctgatccc accttggagg cattcttcgc cgcgacgggc
     2161 gcgcagatcg acgtgaccga ggagccgcgc gcttattaca acataacgac cgaccgcatc
     2221 cacatgccgc cgatcgagac attccaccgg gtgacgggat attacggcac attggcgcat
     2281 gaggtggcga caccaccttt gtcgttgccg ataatgatcg tgcgcatatc agttcctctg
     2341 atcgttatca tcatgggggc aatgcccgct gttggccgtc ttcccaaatt cacggaaagg
     2401 cgagtttcgg cggatgagag tatggtccaa gatcgttagc gcatcgcggt tggggagacg
     2461 ctcccagtca ccgaggcttt cgccaatgtc tttcaacatc tcaaacagaa ttggatcgtg
     2521 ccgggccttg tcacgttcgc gggtgggtac gagtacgaac gcttccaggg acgcggccag
     2581 ccaccggctt ggaacgccac gactgcggca agctcgccat aaatcctgtt gattgcttga
     2641 aacagcgagt ctcccgcagc ctggaggtgc ttggcatcca tggatgcctc gaacgcgctc
     2701 agttcagttt gccagttttc ttctagagcg cagagcgctt caggccctct tgcttgtgat
     2761 tccgagggca tgacagcgcg acctaaaaat ttgcgcatgg ctttgatgaa gaagagcatt
     2821 cgggtcccct gttgtgcgaa caggctatga tgtcctaaac aactatatta tcgctagcgc
     2881 gagtcaataa taaatgtgaa gttgacatga agtcattttc gacctaatga tggacgcgca
     2941 agattagttg ttggagggcg gcgctttctt ttttttcttc tgctggccgt gttcagagga
     3001 cttcgagagc gaagagctca ggcgcttttg cgaggtttgc taccgagtcc gggcctttaa
     3061 gcccaccttg actgtgagcg ggttgcacgc cgcgttgtct gttgctgcat cgtatcctcg
     3121 cgccgtggct tatgagcggt ttgcgagcct aactgatcaa ccgtacaact ctgctgccat
     3181 acaggcagct caactatctg acgggcgtgg gaagctgaca ggtttgcggc ttttgcgtcg
     3241 tgtcccgggg gcggacagga aacagaagat gctgcttccg tcgcgcacag gacgggcgat
     3301 agctcggctg ttttgcgatg tccgtggtgg ttctgatcag gactgcgagc aatatctctt
     3361 tgagacgatt ttgccagtgt ttcagttggc tctacagaac gcgcccagta taaaactcgg
     3421 aactttctgt gtgctccttt ccgttgctca aaatgctgaa aggtttgggg ctcggggtga
     3481 cccgtcaggt ataatctcgg aagaactagg gctttcgaac cttccaaaac atatggagaa
     3541 cttgtcttcg ggttcagacg caaggcccgg gcttgaactg gttgagttac aacgtcatcc
     3601 gctcaatcgg cgaatttcgt tgccaaaact aacagaaaag ggattacggc tcgtggcgaa
     3661 tatcgcggct gccttgcagc ataagccgcc aagtgcagta cgttttccga aagaggaaaa
     3721 gctccgcgat gctccttcac cagacgacgt gaaggcgttt tccgatgacg atttcgactt
     3781 cgacgagatc gagtggcttc gccctgatga tgacacatga aggttcctcg ccgttccggg
     3841 aagtaacgcg actgctcctt gatattgccc gcgccccgcg ccaagtggtc atgaccgcca
     3901 tcacctgcca cggggcggca aggcggtgct atgaacatgg ccgcgaaatg gggtagatgg
     3961 aggacaggcg gcgaatccac cgccgcctgt cctctgcccg atcccgccac cccgttcttc
     4021 tgtaccctct gtgcccatgt cccgtttcct cgcccgccct gtctgcctga tcgccccgaa
     4081 ccaccgtact ccggtcatgg tgctgttgcg gggcgtatct tgtcacagcg ccacagtcgc
     4141 ctgccgtggc atgatctcag gtggcctttt acgccatggc tccggtactc ctcacccgca
     4201 agcctcatca acctaagatg aaaatgccag cacagatatc atcgcacctg cccgaacgtc
     4261 tctctgagag gcttgttggg ctgagccgtg ggcgcaagcg gatgatccag ctggcgatgg
     4321 atgctggcct gatccgcgcg tctgctgtcc gaggcgccga cgggctatgc gacgcggcag
     4381 agcctgcctg cgtgaagatc gggcgtgaag agtggcgtga gcaatgggcg cgatgatgcc
     4441 tgagacccag tccacgacag gaaccggacg ctggctgctg accggcgcct cgggccgcat
     4501 cggccggatg ctgatgcggc actggcagga ggcgccgccg ccggccgatc tggtgctgca
     4561 gagccgtcgc gaaggtggtg gcctgctctg ggacccgctg ctgcagccgc tgcccgccac
     4621 tgctggccgg ttctcctgtc tcatcgcctt cgccggcgtc acccccgccg ccggcgccgc
     4681 gcttgacgcc aatctggccc tggcggaggc gaccctggcc gccgcccatg ccgccgggat
     4741 cgcccgggtg ctgctgacct cctcctcggc ggtctatggc gcgccgcagg gcgatgcgcc
     4801 cttccatgag agcgacgccc tgcgcccgct caacccctat ggcgccgcca aggtggcgat
     4861 ggaggccgcc tgcgccccct ggcgcgcccg cggactggag atctgcatcc tgcgcatcgg
     4921 caatgtcacc ggcgccgatg tgctgctgct caacggcgcc cgcgccacgg ccgcggcgcc
     4981 gctgcgcatc gaccgcttcg ccgatggcca gggcccgcgg cggtcctatg tcggccctgc
     5041 cgatctggcg cgggtggtcg agagcctggc cgcggccgag ggccccctgc ccccggtgct
     5101 gaacatcggc gcgccgcagc ccgtcgacat ggccgatctg gccgatgccg ccggcatgcc
     5161 ctggatctgg accgccgccc cgctgcaggc ggtgcagaac atcacgctcg attgccgcgc
     5221 gctcgacagc ttgcatcccg ccaaccggac cgctagcagc ccggtagaaa tggtccgaca
     5281 atggacccgg ttgaaggacc cgacatgacc ccagccaagc ggatattcga catccttgtg
     5341 gctgtggtgc tggggatcgt gctgatcatt ccggtgctgg tgaccgcact tctgatcctg
     5401 atccgcgatg gccgcccggt gttctacatc tccgagcgca tgaagagccc gaccgagggc
     5461 ttcaacctgg taaagttccg caccatgaag gtggtggcca gcgacagcgg cgtctcgggc
     5521 ggggacaagt ccgcgcgcat caccaggacc ggcgccttcc tgcgccgctc gcggctggac
     5581 gaggtgccgc agctctggaa cgtgctgaag ggcgacatca gctttgtcgg cccgcgcccg
     5641 ccactgcgcc aatatgtcga acgctttccc gagatctacc gcgaggtgct taagtcgcgc
     5701 ccggggatca ccggcctcgc ctcggtctat ttccatgccc atgaggaacg gctgctggcc
     5761 cgcgcccgca ccaccgccga gaccgacgcc atctattcgc gcgcctgcgt gcccagaaag
     5821 gcgcgcatcg acctgatcta ccagcgccgc cgcaacatct gcgtcgatgc cggcatcatg
     5881 ctgaagacgg tgttcaagcg gctgaagtga acccgacaat ccagaaggac attccatgaa
     5941 ctggttgatc accggcggca gcggcttcat tggctcgaac ctgtgccaat ctctggcgcg
     6001 ggatggcgag agcttccgca tcatcgacaa gcgccccagc ccgttctttc ccggcaaggt
     6061 catcgagatg gacattctcg atcaggagca gctggtccaa agcgtgagcg gtgacacgat
     6121 tgtccatctg gcggccgagc accgcgacga catccgtccc cgcgaccgct atgatcaggt
     6181 caacgtcgag ggcacgcgca atatctgccg cgccgccagc ctgcgcggca tcgacaggat
     6241 agtcttcacc agctcggtcg ccgtctacgg ctttgcagca ccagataccg gcgaagacgg
     6301 ggcgatcgac ccgttcaatg actatgggcg caccaagttc gaggccgagg aggtgctgcg
     6361 cgcctggcag gccgaggcgc cggagcgccg cagcctggtg atcatacgcc cgacggtggt
     6421 gttcggcccc ggcaatcgcg gcaatgtctt caacctgatc agccagatcg cccgcgggcg
     6481 cttcgtgatg gtgggcgtcg gcgagaaccg caagtccatg gcctatgtcg agaatgtcgt
     6541 ggccttcatc cgccacatgg cgaagtccgg ccccggcgtc catctgagca actacatcga
     6601 caagcccgat ctgagcatga acgaactggt ctcgcatgtg cgcgcgaccg ttctaggcaa
     6661 gcccggtgtc ggcctgcgcc tgccgtcctg gctgggcatg ggactgggcc atgtcgctga
     6721 cggcgtcacc cgcatcaccg gcaagcgcct gccgctcagc gcgatccgcg tgcgcaagtt
     6781 caccaccagc accgccttcg ccacgcgcgc gcatcaggta ccgggcttcg tcgccccggt
     6841 ctcattggcg gatggcatgg cccggacact ggacgccgag ttcctgcatc ctgaagccac
     6901 ccgcccgatc ttctatacgg agtgatcggg ctgccggctg tcctgccgca cgccgcaact
     6961 agcgccgcag ggccgccgct tcgtagagcc aggattccag gtcaccgtag acctgcgccc
     7021 ctgagtaata cgcctcatag tatctgcgcg cgttaccgcc catcgcttgg cgctcggccg
     7081 cggtgagatc catcatccgt ccgacagcct cggccattgc ccttgggtcg gaaggaggtg
     7141 cggcgaaccc cgcctgcgcg gtctcgatga tgcgcgcgcc ctcgccaccg atggccgcga
     7201 ggatcgggcg accgcaggcc atgtagcact gtaccttgta cggaacgatc ctgttgaaga
     7261 tgtcgctgtc acgcagcgac acgatcaagg catcggcatg ggcaaagaac cccggcatgg
     7321 tttcttccgg gtgccgcccc agcagatgaa agcggtccgt gatccccagt tcggccgcac
     7381 gagcacgcac ccgctccaga tcccgtccac tgccaataat cacccagtgc agcgccccac
     7441 ggtcgcggag cagatccgcg gtggcaagca gggtgtcaaa gtcctgactc tcaccgatat
     7501 tccccgcaaa catcacccgg aaaccaccag caggcatcag aagctgtgca tccggcgcct
     7561 cctctggcgc catcgggcgg tacatatccg gggcggtatt gggcaggaca cggatgcgct
     7621 catccacgat cccaaagcgt gcaaccattg gcgggaaggc tgcgctctgc accagcacca
     7681 gatccgcgcg tcggaagatc cagccacaaa cggcgctgag cgccttcaca atgatcgggt
     7741 tcttcaaccg cagcatgtca atggccgtct cgggccaaat gtcctgcacc caatagacga
     7801 ccggcacgcc gcgcatccat ctcaggacaa tcgccggcag cgcctgaaag ggcggcgacg
     7861 gcatggagac gaaggacaca tccggccgcc gtttcacctt ccgcagggcg gtccatgacc
     7921 cggtcaccgg gaaggtgatg taattcagca ggaggcgcag cttgcttttt ccccgcgcga
     7981 cagaacgtgc gcggtgaatt cttgccccac gccaatcctc ttcgcgggct tcgctgttgg
     8041 agtagccctc gaagaaccca tcccgcccgt agttgggcac acccgtcacc acatcgacat
     8101 cgtggccccg ccggaccagc tcttcgacga tggcattgtt gctgaactgc tcgggaaaga
     8161 aatattgcga gacgaaaacg atcgagagcc gcttcttccc gtcttgctcc gccgcgctca
     8221 tgtcagatcg ttcatccgga tcccatccca cccgttcgac agccgggccg tgccgaggat
     8281 caggctcagc acccgttcgg aggtgttcgt gatcgcgtaa tccgcgggca gcggatgacg
     8341 gatgccggca gccttgcgtt cggcatgcag ctgggtcgcc gcggccacgg cttcgcggat
     8401 ggtgtcgcga ttcaggccgg tgacgatgat gcagccaacg tccaggcctt ccgggcgctc
     8461 gatcgcatcg cgcggcgtga tcgccgggaa ctgcaggatc gagctctcct cggcaatcgt
     8521 gccgctgtcc gagatggcgc agaacgcctg catctgcagg gcattgtagt catggaaacc
     8581 gaacgggacc atgtactgca cccgctgatc gatggcattc gtgccggccg ctccagcagc
     8641 cagagcctcg agccgcttgc gggtacgcgg gtgcgtcgag acgatgaccg gcatgtcgta
     8701 gtcggcggcc agggcattca atgtgtcacg cagcgaggcc aatctctcgc cattgtcgac
     8761 gttttcctcg cggtgcagcg agacgatgaa gtagccctgc ggcttcagcg agagccgcga
     8821 cagcacgtcc gaggcctcga tccggtcacg gtagtgctcc agcacttccc gcatcggcga
     8881 gccggtcagg tagatccgcc ggtgctggat gccctcggac agcagatgcc tgcgggcgtg
     8941 ttcggtatag accaggttga agtccgagat atggtcgacc agcttgcgat tggtttcctc
     9001 ggggacattc cggtcgaagg accggttgcc ggcctccatg tggtagaccg gcaccttcag
     9061 ccgccgcgcg atgatcgcgg aaatcgcgga attggtgtcg cccagcacca gaaccgcatc
     9121 gggcttctcg gcacggatca cggtctcggt ctcgatcagg atcttgccca gggtctcacc
     9181 cagagaccca ccgccggtgc cgaggaaatg gtcgggctga cgcacgccca gatcctcgaa
     9241 gaagacctcg ttcaactcat agtcccagtt ctgaccggta tggacgatgc gatggtcggt
     9301 gaaggcatca agccgtgcca tcacccgcga caggcggatg atctcgggac gagtcccgag
     9361 gatcgtcatc actttcagct tggccatggt cagtcctgca gaacgggatc aaaataggtg
     9421 tcgggagcgg agggatcgaa gaggtcgttg gtccagaaca aggtcaacag cggccgatcc
     9481 ccgatgttct ggatggagtg cgtgtgcaac gtcggcatgt caaccggggc cggggtcttt
     9541 ccgctcacct ggtattccca gaccggccca cccagcacct tgcggatccg gatcatcgcc
     9601 tcgccttcaa gcaccaggaa cctctcgacc ttgccaagat ggaagtgatc gcctcgcgtc
     9661 acacccggct cggtccagct gaggaaggtc tgccctccac cgccgccctt cgccgcctcg
     9721 aacagcgtgc cgcgcgggtc ggcgttcacc ttcaggctgc ggggaaatcc ggcgggatag
     9781 agcgccgcgc ggtagctgtt gaacagatcg cgcaggaaag gatccgcaag atccgggaac
     9841 aggttcgccg cataatccgc atggaagcgc tggagcgtct cgaacaacca gggcacgcct
     9901 gtaggccgcg gcggcggagc cagttcgccg tcatggcgcg ccaaggccgc gtcgatcgcg
     9961 atctgtgccg cagccccggc gtggagcagc tgcacccggc cgttcggatc aatctgcggg
    10021 gattcaccgt tgatcaccgc ctcgatgaag gtggcggtga cattgttgta gcgagggcgc
    10081 gcgccctcgc caaagatgtg cggcaggatc aggtcggtga agccaccccc gatgccggca
    10141 agcagctcgc ccgccagacg cttcgatttg ccatagaccg tgtcgagctt cgcatgggtc
    10201 gagttggcat aggtgacatg cggacgggcg ccggcctgcc ggcaggcatc gaccagactc
    10261 ccggcaatct gcggattgcc gtcacgtacc tcggcctccg tgccgcgatt gaccccggca
    10321 aagtgcagga tggcgtcggc tccggacacg gccgccacga gctgggcgtc atcttcgaag
    10381 gtcccatggt tgagagcgac tatgtcgtaa ggcacggctt ccttgcggaa cctggccgcg
    10441 caattggccg catgcagccg ggacgcgctg tgccagccca gcaacccgcc cgcaccggtg
    10501 acgataatac gacgcgacat caaccgacct tccgagcagg aaccagtcca ccgaaaacct
    10561 cagcgatcaa ggtgcgacac atctgttcta cctctcctgt ccgggcagtt ttccgcgcag
    10621 cggatctatc gcgaaagcgt atcgggggaa accagcaaat acgcgctgag cgcaggtccc
    10681 ccacgtgaca ggacttgaag tccggccatg ccatgctatc gctcaaactt gaacaagcac
    10741 ccaccacatg cggtcgcggc gctcaacgag ggcaagtgca agatgtcgga atatcgcgac
    10801 aagacgcaag aagaaatccg attccggatt ctgcgtctga tcgaacaaaa tccgggcatg
    10861 tcccagcgtg agcttgcagc ggaagtcggg atcagcactg gcggcatcca ctacgttatc
    10921 aaagcgctga ttgaaaaggg actcgtcaaa ctggggcgct ttgccgtagc ggaagataaa
    10981 cgccgctact cttatgtgct tacgcctaag ggcttgacgg aaaaagccgc aatcacacgt
    11041 gtatttctgg cacggaaaat ggaagagtat gagcttctga agcacgagct aaagactttg
    11101 agtgaagtcg tgcgtcgaga tgaaaatctg ggcgacgtct caccatgatg atcggcacac
    11161 gtcttttcaa tgtcggactg cgcagcctta cgctaggcgt tcgcttcctg ttcatattct
    11221 ttcttgcccg atacctcacc ccagagacgg tcgggtatta tgggctattc actgccactg
    11281 tcggttactg cctctatttc gtggggctgg atttctatac ctaccttacg cgagaaattc
    11341 tgaaatcacc tcaggatcgc cgtggcggtc ttctgaaggc acaagctggt ctttctgcaa
    11401 gcttgtttct ggtcctcatt ccagtagctc tcattgcgct accatatgca ggttggccgc
    11461 ccgccctgct ctggtggttc ctaccgatcc tcctcttcga atatctcaat caagagatct
    11521 atcgtttgct gatcgctctt tcacgacaga tccctgccag catcctgctc ttcatgaggc
    11581 agggaagctg ggcaattgcg acgctattgg tcatgggcat gatgccggaa gcgcgcaatc
    11641 tccacgtgtt gatggcattt tggggaataa gcggtctgat gaccgcgatg ctcggcctcc
    11701 tgctcataca ccacctgcga ctggggggat ggcgtgatac catagactgg cgttggctct
    11761 ggcggggcat ttttgtcagc gcggccttcc tggtggcaac actggcgctg cgcggcatgc
    11821 agactctgga tcgctattgg ctcgagagct tgggaggaat cgaaagcgtc gcggcctacg
    11881 tactattcct gggggtggcg ggcacactgc tgacatttct tgacgcaggc gtattcgcat
    11941 ttggctaccc tgagttgatt catcagcaca atatcggtga caccgacgcc acgcgcacca
    12001 cactgcggaa aatgctcatc caaacgctga tcatctcggc gggattcggt atcgcaagct
    12061 ggctggcatt gccttacctt ctggactgga tcggcaagcc agtttacgag gaccggcgtg
    12121 aaatattccc actgctggta cttgcgatga tagtaaacgc tctcggcatg ataccgcatt
    12181 atgcgctcta tgcatctcga cgtgaccggc ccatcatcca cagccacctt gcgtctctcg
    12241 cggcttttgt gctgacgaca tggcttctgg cgttgaccct gccacaaggc aaactagagc
    12301 tcgcaatacc cctagcgctg aatgcaggtt tccttttgat ccttctatgg aaaactctgg
    12361 cgctactctt tccccgcggt gccgatccgg cttcgcaatc ctcacaaccg acgatggcga
    12421 atttcaaatg atccgatacg gccagcaaga tatcacccaa gcagacatcg acgcggttgt
    12481 cggtgtcttg aaatccgtca atttgacgca gggaccctcc attcccgctt ttgaacaggc
    12541 agtcactgcc gagacggggg cgcgacatgc cattgcagtg aacagcgcaa cttccgcgct
    12601 ccacattgca tgcatcgctc tcgatcttgg ccctggcgac gtgctttgga ccacaccgaa
    12661 cacctttgtg gcgtcttcga actgcgcact ctactgcggc ggcgctgtgg atttcgtcga
    12721 catcgatcca cgcacctgga atctctgccc ggatcgtctt gaagagaagc tgatcgcagc
    12781 cgagaagacg ggacgactcc cgaaaatcgt cgtgccggtg catttgaccg gccagccctg
    12841 cgacatggca agaatccatg ctttgggaca aaagtacggg ttccgcatta ttgaagatgc
    12901 atcgcatgca attggaggcc aatatcgtgg cgagccaatt ggaaattgcc gttacagcga
    12961 catcaccgtt ttcagcttcc acccggtcaa gatcatcacg accgccgaag gtgggatggc
    13021 catgacaaat gatgatcact tggccacacg cttgggcctg ctgcgcagtc atgggatcac
    13081 gcgagacgcc gcactcatgg atggcgattc cgatggaccg tggtactacc aacaagtgga
    13141 tctcggctac aattaccgaa tgacggacat gcaggccgta cttggcgtca gccagatcag
    13201 ccggcttcac ccttatgtcc gtagacggca cgagatcgcc gcgcgctaca atgatatgct
    13261 ggccgacctt ccggtcacat tgccttggca acatccggac agctactctg cctggcatct
    13321 ttatgtcatc agattgcaac tcgatcgcat tgaagccagt catcgccagg tattcgaggc
    13381 cttgcggtca cgcgatatca tggtcaattt gcactacatt ccagtccaca ctcagccttg
    13441 gtaccggaaa atgggcttca aggacggcga tttcccgcaa gcggaacagt actatcgcga
    13501 agccatcagc attccgatgc accccagctt gaccgacgcg gaacaagatt tcgtcatcga
    13561 cgtgctgcgc gacaacctga aatgaagctc gcgctgggaa ccgttcagtt cggcgttgac
    13621 tacggtgcat ttggtaccgg caggcaggtc gcgcgcccac aggtcacgga aatactggac
    13681 ttggcgttgg ccgcaggtgt tgatttgctg gatacggccc gagcctatgg cgaggctgag
    13741 gcggtcatcg gagcgcagcc agcatcagag cagttccgca tcgtcacaaa gtgcccaccc
    13801 atcgcccctg aggttgatgc tgccgatgct ctgctcgcgc agtttgaggc ctcttgccgc
    13861 gcgctagggg tcaagaaagt tgcaggctac ctgctgcact ttgcaggaga tatcgcgcga
    13921 cccggggtcg tcgatgtcct tgagagcttg gtcgccgacg gtcggatcga gcggttcggc
    13981 gtatccatct atgacttcgc agaggcggag gaaatctgtg ctcgctaccc agtgacgctg
    14041 gtccaacttc ccgcgaacgt gcttgttcct tggttccgcg acagaggcct cccggagact
    14101 gtcgaagtcc atgtgcgatc ggcctttcta caaggctttc tcttgagtga tccccttggc
    14161 ttgagcgatc gtttcgtgga gtggcgcagt gttctcgaga ccttccgcca tcgcgcgggc
    14221 gctcttggcc tgagccccca tcaggcggcg cttgcgccac tgttgcacag tccgtatgtt
    14281 catcgcgtcg tcgtagggtg cgacacgccc gcacaactga cggaactcat ttcggcagca
    14341 gcaaccgggc cagcggatct cggcgactat gccacagtga cacccgaact gaccgaccca
    14401 cgaaactggc agaggagcag aacgtgagca acgtaactac gctgcaggat gaccctcgac
    14461 agcatggtga cggcgggcgc atctatctgc ggaagctcgt tccggatgat gtcggtgaag
    14521 cctatctcgg ctggttccgt gaccccatcg tcacggagta tttggactct cgcaacctct
    14581 cccgggagga tgtcgtcagc tacatgacgg aaggacacgc gagtggtctg catgccatgt
    14641 atggcatctt tgttgcgggc accgatcaac acatcggcaa tgtgaaggtc gggccaatcg
    14701 cctggaaaca tggcacaggc ggcttggtga ccttcatcgg agagagagat ttctggggaa
    14761 aaggcatagc gcgcgaagcg gtccgcatcg gaacgcgact ggcttttgat atccatggat
    14821 tgcgcaagct gcatgatggc gttgcggatg ggaatatcgg ctcactcaag gcataccagg
    14881 cagcaggctg gatagttgaa gcgcgcatgc ggggccagca tatcgtcaat gataacgtgc
    14941 gggatcgtat cgtgatcagc gcgttcaatc ctcgctattt tccccccgaa taatgatcgc
    15001 gaagttggag tagatcatcg tgaaagtgac ggcaattgta caggcgcgca tggggtcatc
    15061 tcggctgccg ggcaaagtga tgcgcaaggt tggcgaccac gtgctgatcg atcttctgct
    15121 cacaaggcta gcgcgagcaa ggaccgtatc cgagattgtc ctggcgacca cagaccaacc
    15181 cagtgatgac agactggcca agcacgttgc agacctcggc tacaaggtgg tgcgaggaag
    15241 cgagaacgac gttctgtcgc gcttcgtgtt ggcagctgat gccactgatg gtgacatcct
    15301 cgtccgcata accggcgact gcccactggt catgccagag ctggtcgaca cgttggtgca
    15361 aggatatcaa caagccgatc tggactatct gtccaatgta gatcctccga catggcccga
    15421 cggactggat gtagaggtgt tcagcagcac cgctctgcgg cgtgcggctg cagagacgtc
    15481 ggagcaattc gaccgcgagc atgtcacgcc cttcttgcgg cgtggcgact ttcggcgggc
    15541 aaacgtcaca aatgcccaag acctttcgaa gttgcgactg acggtcgatg ctgcggccga
    15601 ccttgaggtc atggagagtt tgatcaaccg tgtcgaagat ccggtcaatc ttgatctgga
    15661 cgcattgctc gcgctcacaa acagtgagcc ggaacttttc gacgccaatt caatgcttaa
    15721 cagaaacgag tatgcgatga ccggacgtgg gcagaagctc tggatgcgcg cgcgccagat
    15781 cattcctggt ggcaacatgc tgctttcgaa gcgggcggag atgttcttgc ccgagcactg
    15841 gcctgcgtat ttcgaccgta cggagggatg cgtcgtctgg gacctcgacg gcaactcata
    15901 tctcgatctc ggtctcatgg cgattgggac caataccttg ggctactcgc atccggacgt
    15961 ggacaaagcg gtcatgtccg tcgttgccaa agggaacatg tccacgctga actgccccga
    16021 ggaagtgctg ctggcggagc gcctgataga gatcagctca ccttggggtg agatggtgcg
    16081 ctttgcgcgg tcaggtggag aggccaacgc gatcgcgatc cggattgccc gcgccgcctc
    16141 cgggcgtgat ggggtcgcag tctgcgggta tcacggatgg catgactggt atctctctgc
    16201 aaatctcggt gacagtgaga atctggccgg gcatttgctt cccggcttga atccagccgg
    16261 cgtgccgcgc tcgctaggcg catccacttt ccccttcatg tacaatgatc tcgcgcgcct
    16321 cgaggaactg atcgcgacga agagcatcgg cgtgatcaag atggaagtgt atcgcaacat
    16381 cgagccgcag gacaactttc tccagaaggt tcgtgatctg gcgactcgaa acggcatcgt
    16441 tcttgtattc gatgaatgca cttcagggtt ccgcgagacc tttggggggc tctacaagaa
    16501 atacggtgtt gagccagaca tggctgttta cggcaagact ctcggaaatg gctatgcaat
    16561 tacagctgtt gtcggaagtc gcgacgtcat ggaggctgcg caaagcacct tcatcagttc
    16621 caccttctgg acggaacgca tcggccccgc cgcggcactt gcaacccttg acgtcatgga
    16681 gcgcgagaag agctgggaag tcatcacgga gactgggcga gtctggcaac gcagattgca
    16741 gcagactgcc gatgaagcgg cacttccaat ctccatcagc ggcctgccat cgctcagcac
    16801 ttattcagtc cttggcgatg acagcgcaga gtgcaagacc tacatcacgc aagagctgct
    16861 gaagagaggc atactggcag gtaccggcct ttatgccagt attgcgcacc agcccgcaca
    16921 cctggacaaa ctgttctccg aactggctgt gattttcgca aaaatcactg acaccagagc
    16981 ggccgggaaa acagtggcag ctctgctcga tgggcccgtc tgccataccg gcttccgccg
    17041 gctgaattga gcagcagaag ttgtcgagtg tagtcatccg cacagatgcg gccacaatga
    17101 tcggcaccgg gcatgtcatg cgctgcttga cgctcgccga tgagttgcga gacgaaggag
    17161 gagccgaagt gctcttcctt tgccgcccgc tggagggaaa tctcatcgcg ctgatcgccg
    17221 cccgcggcca tcgcgtgatg acactgcctg cattggggtc tcacgtgccc tcctcgaccc
    17281 gtcccaccga ttggctgggc accgatcaag cgacagatgc tgccgattgc caacgcgcct
    17341 tggcatccca agcgattttt gactggctga tcgtcgatca ctacgccctt gatcacgagt
    17401 gggaaacgca gatgcgcccg ctctgtgaca ggctgctagt agtcgacgat cttgccgaca
    17461 gatcacatga ctgcgacctt cttctcgaca cgggtcttgg gcgcacggat gccgactatc
    17521 gcgcgttgat ccctgatcat gctctctctc tactcggccc gcgctatgcg ctgctgcgcc
    17581 cagagttccg agcccatcgg gcgatcagcc tttcacgacg cgatgcgccg gtcctgaagc
    17641 aagttctagt gacactgggc ggcaccgacc cggcgaatat gacctgccag gcgcttgatg
    17701 ccttggacgc aactgctcta cctctcgaga cggaaataag cgttgtcatg ggcgcgagtg
    17761 cgccgtggct cagcgacgtc aggcgacgtg ccagcacaat gaaacaatcg acgcgtgtcg
    17821 cggtcgacgt gaaaaacatg gcagaaataa tgactgacag cgatcttgcc ataggcgccg
    17881 cgggcggtac ggcctgggaa cgctgctgtc tgggactgcc atcgatccaa ctggtactcg
    17941 cagagaacca aagaggaata ggtcaatctc tccaagcctc cggcgccgca gtgagcgtcg
    18001 tgtcaccaag tgagatcacc gacttcctaa cagaattgac cgcgcgcaac gaacttcact
    18061 catggatgaa gcagagcgct gccgcggcgg cggccattgt cactggcaac ggcgtatcag
    18121 ccgtcagatc gtcgatgatg gcccacaatg cttgagcaat gcagcatacg agaggtcgag
    18181 agaacagacc tccccactct cttgtcgtgg cgaaaccata tcacggtgag atcagtaatg
    18241 ttcaatcaga acgagatttc ctcagatgag caccataatt ggtttgttcg cgtaaaagag
    18301 gactccaatc ggcagcttct gattgcagaa gatgaaagcg gactactggg gtttgcgcaa
    18361 tttacaggcg tagcgcgcgg tggcattgcc gaatgggggt tctacgcccg ccccgacgca
    18421 ccaaaaggca gcggcacaaa gctttgcagc ttggccctgg atcatgcatt cgaaagcctt
    18481 gaactccata aggtatatgg ccaggcaatc gaaaaaaacg caccttcggt ggctatacat
    18541 cgaaaactgg gcttcactga agaaggtgtg cttcgggatc acagacacgt aaacggcgcg
    18601 tatgtctcac tggtgtgctt cggagtactg aaccatgagt gggcgacaaa gcggaatagg
    18661 agagaagtat gaacggcata acaattgatg ggcgaaatat tggcccaaat caaccaccat
    18721 acgttattgc tgaactttcc gcaaaccata acggcagcct cgaaacagcc ctgcgcatca
    18781 tagatgaggc ggtaatcgca ggcgcagacg ccgtgaaact tcagacctat cgcgcagaaa
    18841 caatcacact cgacagcgat agtgaagagt tcgtcattcg cggcgggctc tgggacggaa
    18901 aacgtcttca tgatctgtat accgaagcac atatgccatg ggattggcat cagccactct
    18961 tcgatcatgc ccgcaaacga ggcatcacca ttttctcatc gccatttgat agtacagcta
    19021 tcgacctcct cgaggacctg aatgtgcctg cctacaagat tgccagcttc gaagctgtag
    19081 acctgccact aattcgatat gcggcgtcga ccggcaaacc aatgatcata tcgacaggta
    19141 tggcggatgc cgaagaaatc ggcgaagccg tggcagctgc caaagacggc gggtgtcgcg
    19201 aactggcaat cctgcattgc gtctctggct atcccgcccc tccgggcgac tacaacctca
    19261 gaaccattcc tgacatggtg gaaaggttcg ggctggtcac gggcctgtcg gaccacactc
    19321 tcgacaatac cacagcgata gcaagcgtcg tactcggtgc ctccatcata gagaaacact
    19381 tcacattgaa tcggaatggt ggcggacctg acgacagctt ctctctggag ccaccagaaa
    19441 tgaaggcact ctgccaaggt gcacgtaccg cctgggaggc actcggacgc atcgactatg
    19501 gacgcaaatc gagcgagcaa ggaaatgtac agtttcgcag atccctttac tttgtgaagg
    19561 atctgaaggc gggagaagtg atcactccag attcagtacg cagtgtcaga ccaggatatg
    19621 gactggcccc caaatatctg gagaaaatac tgaatgccaa agtgaagcaa gacgtatcaa
    19681 agcacagtgc agtaacgtgg gagaaactga aaagcccaga ggaataatat aggcgagaaa
    19741 agcaatgatc agaatctctg ccctcctcac tttggcggcg ccaacttaca tagcactcac
    19801 attcacaaga ttctatgtag ttaatccaca aactcaaagt atcattggca tacttctggt
    19861 cgcaactggg tgcggcttca tgatggcatc aatgtcatcg ttccacatcg gtagatgggt
    19921 gctagcttcc gttgcagtca ttgcaagtgc agcggcaata aacactttag taggaaacaa
    19981 cttctcaatc gatagtggag tgcagctcct agctaacata gggtatgcgt atgcgatatc
    20041 gaaaggaccg atgtccgtaa ctgctcaaaa agtcataaca ctacttctat ctgctttctt
    20101 catgtcgcac gcggtattgg gagtaaatcc cgaaggcatc tttacgatta gcaggaactt
    20161 catatctgta ctcatgatcc tttccgtcgc gttctactat atcgcctgca caaagaatga
    20221 gaagtcccca gaggttctga taccaatttt tgtcctagcg atatgcctat gggcaattgg
    20281 gagatctggg attattagcg cggcgcttct tctagtcggc accaccgtcc tctccaacaa
    20341 aaagacaacg atactcgcgg tatatagcct gattgccatt tctgtaattg ccgttgcggg
    20401 tgaaagacta cttgcatacg tggagatatt tcaggtggga atcgaaagat ttgaaagact
    20461 gtctgcaagt ggccccgacc cgcgcgagct gatatttgat gagtacttcg caattacttc
    20521 gacacatctg aagtacatat tctttggaac gccactaggg ctggtaagcg agatctcggc
    20581 agtgggagga aaccctcaca atgcatatat aagcctgcac gcctcgttcg gaatgttggg
    20641 gcttacttac ttcatgctac tctccgcagt tagcctggca aagctatggc gggccaaatt
    20701 gagatacctc actctcgtat tcctcatctt gctactgcgg tctgcgttcg actcgaccgc
    20761 ctttcacggc cccttggatg tcgcgctgtt ctcatgcata ttcgtcgcgc ttgcgaaaaa
    20821 aaggtatgac gatatgtcat caccgccctt cagcaggata tggaccgcga tgaaactgcc
    20881 aaacggacgt cgacaagcga agaacgagcc gtggatctaa gatggtaaaa atctcgacag
    20941 gcctgcgcgg cctcaaatca aaactaaaga agaccatttc tttgctcctc agcaggattg
    21001 atgtcaattc cgagatccac aggtccacaa acataaatag atttgttgtg tctcgaaaca
    21061 gcaaaatcga ccgctacagt tacgtcggac cccgcacgaa gctggagcgc tctgatattg
    21121 gatcattttg ctccataagt tgggattgcc atatcgggct atcgtcccat gacctatcga
    21181 ccgcttctac gtctccaata ttcacatcag cagtcaatgg ggtcggcgta acctggaccg
    21241 agatatcgac ggaatctcta atgaatcccc gcacgttaat tgggagcgat gtttggattg
    21301 gcgcacgggc gatcgtaatg gagggcgtgg aaatagggag tggtgctgtt gttgcagcag
    21361 gttcaattgt aaccaaggct gttgcaccgt actcaatagt cgcaggggtt cccgccaagc
    21421 acataaagta ccgttttccg gaagaggaaa gacaggctct tcttgacggg gaatggtgga
    21481 acgcagatga agaacaactt aaggcgaaga tcgcggtgtt ccagaaaaaa ataacctccg
    21541 tgagcgatgt gctcatgttg ccg
//
LOCUS       DAORPM010000007        21401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_415_length_21401_cov_10.406493, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000007 DAORPM010000000
VERSION     DAORPM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 21401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21401
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_415_length_21401_cov_10.406493"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PLD53_00720"
     CDS             complement(<1..1146)
                     /locus_tag="PLD53_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HRC59454.1"
                     /translation="MNSTPLFDFDRPIDRRGGDSLKWNKYAGRDVLPLWVADMDFAAP
                     PALLAALERRIAHGCLGYAEPWPSLVDSVLDYLQRKYVWTIDPEWLVWLPGLVTGLNL
                     ACRTVDGSVLTATPVYPPFLSAPRLSGRELMRAPLRLSEGRWGWDFGAMEAALAPDTR
                     LLLLCHPHNPVGRAWGQDELSDLAGFCQRHDLIVCSDEIHCDLILDEGRQHTPLAMID
                     AEIARRCITLMAPSKTYNIPGLGCAFAVIADAALRRRFTGVMRGIVPDVNVLGLTAAE
                     AAYRDCEQWRAALINVLRRNRERVETAVSAIPGLSMAHVEATYLAWIDARSLGVADPA
                     AFFEAAGVGLSNGSDFGLPGWVRLNFGCAGGHLDSALDRIKMACSARL"
     gene            complement(1182..>1694)
                     /locus_tag="PLD53_00725"
     CDS             complement(1182..>1694)
                     /locus_tag="PLD53_00725"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306745.1"
                     /note="threonine deaminase; threonine dehydratase; in
                     Escherichia coli, IlvA is part of the isoleucine
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HRC59455.1"
                     /translation="SLPEKPGAYREFLSLIGARNITEFNYRFNDRKEAHVFVGLQVGS
                     RSESLKLVAQLRKHGYATLDLTDDEMAKSHIRHLVGGHSPQAEHEMVYRFEFPERPGA
                     LMNFLNRMSSGWNISLFHYRNHGADYGRVLVGMQVPPGEMGEFRKFLKYLGYAHWDET
                     ENPAYKLFLA"
     assembly_gap    1695..1794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1795..2468)
                     /locus_tag="PLD53_00730"
     CDS             complement(<1795..2468)
                     /locus_tag="PLD53_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRC59456.1"
                     /translation="MHPDYLEKILNAQVYDVAVESPLDHAPNLSARIGNCLLLKREDM
                     QPVFSFKLRGAYNKIVKLSSEKLRRGVICASAGNHAQGVALSAAKVGCRAVIVMPTTT
                     PLIKVDAVRTLGGEVILFGDSYDDAFAHALLLEKAERLTFVHPFDDPEVIAGQGTIGM
                     EILRQHQKPIHAVFCCVGGGGLISGVAAYIKRLRPETKIIGVEAIDADAMERSLKAGK
                     RVRLDRV"
     gene            2612..6517
                     /locus_tag="PLD53_00735"
     CDS             2612..6517
                     /locus_tag="PLD53_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HRC59457.1"
                     /translation="MIARLREIPYNYTSFSDREIVIRLLGEENWAILDELRSQRVTGR
                     SARMLYEVLGDIWVVQRNPYLEDDLLANRQRRAALIHALRHRLNEIDKRRQGNALVLK
                     LHDSARAAVDAFERHFDDTATLRRRVLKTLLRHTRRDNIAFDGLARVSHVTDATDWRV
                     EYPFVVLYPDSEEELAPLVCGCIELGLTIIPRGGGTGYTGSAVPLTPLSAVINTEKLI
                     DIGPVEEAILPGCEQPCTTIRTGAGVVTARVSDAAAAAGRIFAVDPTSAEASCIGGNV
                     AMNAGGKKAVLWGTALDNLAWWKMVDPDGNVMEVSRIDHNLGKIHEQAVVRFEIRRFR
                     MDGTTPYGEPELLEITGQTFRKAGLGKDVTDKFLAGLPGIQKEGADGLIVAARWVLHK
                     MPPHARTVCLEFFGQVREAVPAIVEIADYFKPGGAGHRNGVLLAGLEHLDERYVRAVG
                     YATKAKRKGEGSGRLKMVLIGDLVGHDEKAVMAAASDIVRLCNLRGAEGFIAVDADTR
                     RRFWLDRSRTAAISRHTNAFKLNEDVVIPLPRMGDYCDGIEHINIELSIRNKLELCDK
                     LADFLSGTLPLNRGDANIASDVLIGDRRQRAMDAVGIVRARWQWLLDHLDQPLEQMEA
                     QFSSFGIFAGTLWCKEENPVLFHRLQDYSIRVSWKAELKPQLEAIFVGNVFRPILERI
                     GAIHAETLRGRLFVALHMHAGDGNVHTNIPVNSDNYAMLQTAYATVERIMALARSLDG
                     VISGEHGIGITKLEFLSEEEIQPFRDYKSRVDPKGHFNKGKLLPGGDLAKAYTPSFSL
                     LGAESLIMEQSEIGNISEMVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILATSLLIE
                     AFLYEEQTRRGISLMHFDEFNDVADHCTICHKCVAPCPVDIDFGDVSVRMRNFLREQG
                     KKKFVPAKAAAMAFLTMKDAATIRLTRKLMIDWGYRAQRMAHQAAKALGLIQDQTRQP
                     PSTLGQPTVRAQIIHFINKPMPGGLPKRTSRALLDIEDERIVPVIRDPHKVNAADYSG
                     DAVFYFPGCGSERLFSQVGLATQAMLYEIGAQTVLPPGYLCCGYPQNAAGEADQGQRI
                     ITDNRVLFHRVANTLNYLDINTVIVSCGTCMDQLQKYQFEKIFPGCRLLDIHEYLLEK
                     GVSLEGVSGTRYLYHEPCHEPMKTMAGIKVVNELMGQNVDLSDRCCGESGTLSVTRPD
                     VSTQIRFRKQQEIEKGVAKLRAEGGMGASGSTTSFTGDIKILTSCPSCLQGLSRYNDD
                     GGTQADYIVVEMARHLLGADWMEKYIQKASNGGIERVLL"
     gene            6713..>7431
                     /locus_tag="PLD53_00740"
     CDS             6713..>7431
                     /locus_tag="PLD53_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRC59458.1"
                     /translation="MRKAVEAGATATPSASRSAKPLPHSLAPDSLGWSLLLAARVLAA
                     VLGGKGLNDALRMLADEPPKARAAAQDVAYGVLRRYGFGEFILGQLMAKPLTHPETQA
                     LLLGALYRLETRADSTPMIVDQAVAAAGELAGGVFKSLVNGVLRNYLRQRKALLAAMA
                     DNDEARHQHPRWWVARLRRAYRDNWQSIVAAGNCPPPMTLRVNQRRTTVPDYRASLEA
                     AGFAARPVGRHGLILERPVAV"
     assembly_gap    7432..7531
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7532..7813
                     /locus_tag="PLD53_00745"
     CDS             <7532..7813
                     /locus_tag="PLD53_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRC59459.1"
                     /translation="VVRRHPDIKTLRRESDIRRFALKQAAILDALWPLLKPGGKLLYA
                     TCSVFPEENAAQIDAFLVRHSGIERLFEEQLLPQDENDGFYYALLRKTV"
     gene            7788..8483
                     /locus_tag="PLD53_00750"
     CDS             7788..8483
                     /locus_tag="PLD53_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HRC59460.1"
                     /translation="MRCCEKLFRAIFCSAALLLTALTTQAAEISIRNPQLEANEDGYA
                     LSADMNINFNTRLEEAVNRGIVLFFAADFELTRSRWYWLDEQVVRRSKIFRLSYHALT
                     RQYRLSTGASALHQSYASLDEALSVMSHVRNWQVLEKDEVKPGQAYLASLRMRLDLSL
                     MPKTFQVSALANRDWNLSSEWLRWDFMPSELKQEPPPAISTPATPSANPHSTPASGTP
                     PASPATPAAGEAK"
     gene            8480..>8829
                     /locus_tag="PLD53_00755"
     CDS             8480..>8829
                     /locus_tag="PLD53_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRC59461.1"
                     /translation="MKNLIIVAASFGGILLFLLASTSANTAMFASNYPWLLGLNAFVA
                     LSLLVLIIWQVRDLWREHQARIFGSRLKLRLMLMFGLMAVLPGVLIYAVSVQFVTKSI
                     DSWFDVRVEKALESG"
     assembly_gap    8830..8929
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8930..10797
                     /locus_tag="PLD53_00760"
     CDS             <8930..10797
                     /locus_tag="PLD53_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC59462.1"
                     /translation="KSIDSWFDVRVEKALESGLDLGRSALDSLLDDLAEKGRVMSLEL
                     GNQSEIQHQLALGRLREQTGVQFAALFSTNGQLLATATSDLGITMPMQPSLEQLRKAR
                     FGRGYREIESAGGTELMLRVLVAVSPMGLGMETRILQLTQPVPSGLARNADRVQEVYR
                     DYQELSLGRQGLTRIYAMTLTLTVLLALFTAIAMAFILARRLSAPLSILAEGTQAVAA
                     GDFTPRQAINSRDELGVLTQSFSQMTRQLDEARRDTERHRSAVESARAYLESILANLS
                     AGVLVFDSNFILRTVNEGAKTILDDQFDDLLAVTVDAWPREQVLGGTIRDAFAEYGRK
                     EWHTQIELDRPGSLPQILLLRGTQLPEGTNGGYVVVFDDVTRLVAAQRSAAWGEVARR
                     LAHEIKNPLTPIQLSAERMQLKLASKLSAGDAEMLDRSTQMIINQVQAMKRMVNEFSA
                     YARMPAPELGRLDLNALISELLGLYESSQNRINIELDAVLPAVWGDASQLRQIIHNLL
                     RNAQDAQEGVEAAQIGVTTRVVEGMAELLVTDCGPGFPPEIMARAFEPYVTSKAGGTG
                     LGLAIVKKIVDEHQGHIRIKNRQPTGAEVSIRLPLAERLTPNPITTTMTETTDES"
     gene            10800..12059
                     /locus_tag="PLD53_00765"
     CDS             10800..12059
                     /locus_tag="PLD53_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC59463.1"
                     /translation="MAQILVVDDEMGIRELLSEILADEGHTVLLAENAAAARNSRSEK
                     RPDLVLLDIWMPDTDGISLLKEWSSAGLLTMPVVMMSGHGTIDSAVEATRVGAIDFLE
                     KPIALQKLLATVKKALKHDVSPQLPPLTLDAFARSPLLKDLKKRLEQTAAKTSLLLLK
                     NASGSIAEICARTLHTPKTPWLDLSASSEPLTQEMLANAAGGILFLADLSVLGKLQQK
                     NLVYALERLEKYNLQLVAASARPLSSLIENGWDPALVTRLSEVWVSLPQLSMHTDDVP
                     EISTLVLSHLIARNEVPSRSFSSGALNALRLHHWQGEWAELLATIKNLALSALDDDIS
                     VDDVDSILLREARDAPEAPPALPLFNQPLREAREAFERLYFEHYLKSEGGNMARVADR
                     TGLERTHLYRKLKQLGLNLGRRGEDTE"
     gene            12094..13524
                     /gene="trkA"
                     /locus_tag="PLD53_00770"
     CDS             12094..13524
                     /gene="trkA"
                     /locus_tag="PLD53_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRC59464.1"
                     /translation="MRIVILGAGQVGASIAQILVSENNDITIVDNDQKRLEYLQVRLD
                     LRTIAGNAVLPSTLRDAGLADADMLIAVTRSDQTNLIACKLAHTMFNVPTRIARLRSS
                     EFMDDAELLTEKNFAVSYALCPEQVITDYLVKLVEFPEALQVLYFANERVALVAVKAT
                     AGGLMVGKPIREMSEHLGGNVDARISAIFRRDHAVTPTGSTVIEEGDEVFVLAADEHI
                     RRVLIEMRQKTTPVQRVIIAGGGNIGLRVAQMLEGKCLVKVIESNTARTQTIANLLGN
                     GLVLLGDATDEELLEQESIGETDLFLALTNDEEDNIMSASLAKAMGCKRVLALINRRA
                     YADLVQSGPIDIAISPALVSIGDLLTHVRRGFVAKVHSLRRGAAEAIELVVHGDEKSS
                     RVVGRRVGDLPVIPGATLVAIVRDFDRIEDIAYAGMLKIKKKGHVVIAHKGEVIKPAD
                     HVIVFCLNAKVVKKVEKLFSVGLHFF"
     gene            13535..14989
                     /locus_tag="PLD53_00775"
     CDS             13535..14989
                     /locus_tag="PLD53_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRC59465.1"
                     /translation="MKVYLPVIRVLSMVIFIFGLTMLVPVGVSWVFQDGAARAFDEAI
                     LLTLGVGLVLWGFTRRNKRDLTIRDGFLMVALVWTVLPVFAALPLIFYLDASFTDAYF
                     EAVSGLTTTGATVFSELDKLPVSINFWRTQLVWLGGMGLIVLAVAILPLLGIGGRQMF
                     KAETPGPMKDSKMTPRIAETAKGLWGVYTGITVCCFLAYHWAGMSWMDAIMHSFSTMG
                     LGGFSSHDASFGYFNSPTIETVSIVFMLIAGMNFATHFVALSGRSIRPYITDPEAVWF
                     LVVALGSVLGIAVFLNENDVYRSFSEAFRYSAFNVVSIATTTGYANTDYYLWPMFAPL
                     WMLFLSSFATSAGSTGGGIKMIRALVLYKQVNRELLRAIHPDAVYLVRIGSEVVPGNI
                     LFAVLAFGFMYMVSIVSMTLLMSLSGLDIITAFSAVVASINNTGPGLNQVGPATTFEV
                     LNDFQTWICTFAMLLGRLEIFTLLVVFTPAFWRR"
     gene            15120..16193
                     /gene="hemE"
                     /locus_tag="PLD53_00780"
     CDS             15120..16193
                     /gene="hemE"
                     /locus_tag="PLD53_00780"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRC59466.1"
                     /translation="MTRPRNDNFLRALLKEPTEYTPIWLMRQAGRYLPEYCEARKRAG
                     SFLQLCKNPSLACEVTLQPLARYDLDAAILFSDILTVPDAMGLGLYFTEGEGPRFERP
                     LRQEWEIRDLTAPDPYEHLRYVTDGVSEIRRALDNSVPLIGFSGSPYTLACYMIEGAS
                     SSDYRHVKTMLYDRPDLMHRILSVTADAVIAYLNAQIESGAQAVMIFDSWGGGLSAAA
                     YPEFSLQYMRRIVSGLIKEKDGERVPSIIFTKGGGLWLESIADSGCDAVGLDWTIDIG
                     EARRRVGHKVALQGNLDPNVLFASPDKIADEAQKVLDSYGSGDTGHVFNLGHGISQFT
                     QPERVTALVEKVHSYSKVLREKT"
     gene            16637..18646
                     /locus_tag="PLD53_00785"
     CDS             16637..18646
                     /locus_tag="PLD53_00785"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRC59467.1"
                     /translation="MQIARVALDVPLHRFFDYIVPETEALSDSDLGRLVRVPFGQKSR
                     IGIIVALPQSSDVPVARLKSIEAVLRDIPALPADWFRLSEFCAAYYQAPLGEVMLSTL
                     PAGLRRVAPPKARTSQRSVKPSPPQEPPELTAEQKTALAAIAEGDQGFKAYLLHGVTG
                     SGKTEIYLRLVERSLAAGRQVLMLVPEINLTPQLESRVAARFPSAGLVSLHSELTEAA
                     RTRNWRAALEGRARIVLGTRLAIFAPLPDLGLIVVDEEHDASFKQQDGMRYSARDVAV
                     FRARERGIPIVLGSATPSLESWANATDHRTPARYTLLTLHQRAVENSRLPLVQRIDTR
                     PEKLQDGLSAALLLGIEKRLTRGEQSLVFLNRRGYAPVLACTNCGWISHCHRCAANLV
                     LHLLDRRLRCHHCGFESPIPKACPTCGNQDIQPFGRGTQRLESVLAERFPQARILRVD
                     RDSARSRKQWEALVQRIYSGDADILVGTQMLAKGHDFPKLTLVGALGADAALFASDWR
                     APERLFAQLMQVAGRAGRAELPGEVLIQTQFPDHPLYAALVQHDYAAFADSQLKEREQ
                     AGFPPYTFQAMLRAESPHMADALAFLATARAGPQTGAHVEVMLYDPVPMRLARRANFE
                     RAQLLIESPSRRALQAFLGAWRLVLETIKTPSRLRWHLEVDPLEF"
     gene            complement(18701..19759)
                     /locus_tag="PLD53_00790"
     CDS             complement(18701..19759)
                     /locus_tag="PLD53_00790"
                     /inference="COORDINATES: protein motif:HMM:NF023695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HRC59468.1"
                     /translation="MELTTCLRAVQRAQFALWHVRSMRKVRAVPMLLCALVGAFVTIE
                     SRAFATATEYGGTCDVSAAVSLGEQQFVVANDEDNVLRVYREGRTAAPVSTLDLSEFL
                     KSDPKHPEADLEAAARIGNRIYWIASHGTNSDGKLRSGRRRFFATQINGAGEAVELSV
                     VGKPYRKLVRDLTRLPALKAYKLGDAAKIPPKEPGGLNIEGLAATPDGRLLIGLRGPI
                     PDGKALLIPLNNPQEVVAGKSARLGAPILLALGGRGVRSIDYVPALGQYIIMAGAAGV
                     SGKFQMYRWSGVADEDPVAVNGENFSGLQPEAMIAYPGPPVRMLVFSDDGTREKGSLM
                     CKDLPVGQRRFRTLWITL"
     gene            complement(19814..20902)
                     /locus_tag="PLD53_00795"
     CDS             complement(19814..20902)
                     /locus_tag="PLD53_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HRC59469.1"
                     /translation="MPKPPTDTSQSAPAPAPGFSLEALHPGNFALVMASGIISIGFST
                     LHHELLADVVCVFAIVAWVTLLTLNVGRFFMFSRAVRIDLLNPRMVFSFFTLVAATDI
                     VGLLLNARGHTGLAMGCWVIAFLAWCLLLYLAFSVLTFLSHENNVNIVHGGWLISIVG
                     TQSLVLLGAAIAPALGEYAGYMMVEVHMLWGLGLLFYGIFVTLFCYRIFFLALKPQDI
                     SPLLWVIMGAAAISANAGTSLLSEDPYLPFLAAQRPFVEGVTMMIWAWGTWWIPMLLM
                     FGFWKHVARGLPLKYEPAMWSFVFPLGMYTVASARLGLAAEFPPLQWISQIMIWVAFA
                     VWLFVLGGLARGLLTRLAGVRARTAAPP"
     gene            complement(21080..>21401)
                     /locus_tag="PLD53_00800"
     CDS             complement(21080..>21401)
                     /locus_tag="PLD53_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59470.1"
                     /translation="AAFLNGQPADPALLPQHYRAVLDGGQAALDSIEAPLPRLIAAGV
                     LLRSGRLAPADIDLAVDTASAQGWRRPLLAWLGVQAQLADKTGDHEMAARARRRGELV
                     SGNR"
BASE COUNT         4378 a         6402 c         6103 g         4218 t
ORIGIN      
        1 caggcgagcg gaacaggcca tcttgattcg atcaagcgcc gaatcaagat ggcctccggc
       61 gcagccaaaa ttgagccgca cccagcccgg caaaccgaaa tcgctgccgt tggaaagccc
      121 gactcccgcc gcttcaaaaa atgccgctgg atcggctacc ccaagactgc gcgcatcgat
      181 ccacgccaga taggtcgctt cgacatgtgc catggatagc ccggggatag cggataccgc
      241 cgtttcgacc cgctcgcggt tgcggcgaag cacattgatc aacgccgcgc gccattgttc
      301 acagtcacga taggccgcct cggcggccgt caggccgaga acattcacgt cgggtacgat
      361 gccgcgcatc actccggtaa aacggcggcg aagagccgca tcggcaatca ctgcaaacgc
      421 gcacccgaga ccaggaatat tgtaggtctt cgacggtgcc atcagcgtga tgcaacggcg
      481 ggcaatctct gcatcgatca tcgccagcgg ggtatgctga cgaccctcat caagaatcag
      541 gtcgcagtgt atttcatcgg aacaaacgat cagatcgtgg cgctggcaaa accccgccag
      601 atccgacaat tcatcctggc cccaggcgcg accgaccgga ttatgcggat gacaaagcag
      661 gagaagccgg gtatcgggcg caagcgccgc ttccatcgca ccaaaatccc aaccccagcg
      721 accctcggac aggcgcaagg gcgcacgcat cagctctctc ccggacaaac ggggtgccga
      781 aagaaaagga ggatagaccg gtgtcgccgt caggacactc ccgtccaccg tgcgacaggc
      841 cagattcagc cccgtaacca gccccggtag ccagaccagc cattccggat cgatggtcca
      901 gacatacttg cgctgcaggt agtcgagtac cgagtcaacc agagacggcc agggttccgc
      961 ataaccgagg cagccgtggg ctattcttct ttcgagcgct gccaggagag ccggaggcgc
     1021 agcaaaatcc atatccgcca cccataacgg cagcacatcc cggccggcat acttgttcca
     1081 cttgagcgaa tcgccgccac ggcggtcgat cggtcgatca aagtcaaaaa gcggcgtcga
     1141 attcatgcag gagctgcctt ttcatacggg gaggatctga atcaggccag aaagagcttg
     1201 tacgccggat tttcggtttc gtcccagtga gcatacccca ggtatttcag gaatttcctg
     1261 aactcgccca tctcgcctgg cggaacctgc atgccgacca gcacgcgtcc gtaatcggcg
     1321 ccgtggttgc gatagtggaa cagactgatg ttccagccgg aactcatccg gttcaggaaa
     1381 ttcatcagcg cgccgggacg ctcggggaat tcaaagcgat agaccatctc atgctcggcc
     1441 tgtggcgagt gaccgcccac cagatgacgg atgtgcgact tggccatttc atcgtcggtc
     1501 agatcgagcg ttgcgtagcc gtgcttgcgc agttgagcga cgagtttcaa agactctgag
     1561 cgcgagccaa cctgtagccc gacaaataca tgcgcttcct tccgatcgtt gaagcggtaa
     1621 ttgaattcgg taatgttgcg cgcaccgatc agcgacagga attccctgta agcgccgggc
     1681 ttctcgggca ggctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgcga
     1801 tcaagacgca cacgcttgcc cgctttcagc gatcgttcca tggcatcggc atcgatggcc
     1861 tcgacgccga taatcttggt ttccggacgc agacgcttga tataggcagc cactccggaa
     1921 atcaggccac caccgccgac acaacagaat acggcatgga ttggcttctg atgctgacgc
     1981 agaatctcca tgccgatggt gccctgtccg gcgatcactt cggggtcatc gaatggatgc
     2041 acgaaggtta gccgttcggc tttttccaac agcaaagcgt gcgcaaaggc gtcatcgtag
     2101 gaatcaccga aaaggatgac ctcgccgccg agtgttctga cagcgtctac tttgatcaat
     2161 ggcgttgtcg tcggcatcac gatcactgcc cggcagccaa cttttgccgc cgacagggcg
     2221 acgccctgcg catggtttcc ggctgaggcg cagataacac cgcgcctgag cttttcggaa
     2281 gagagtttga cgatcttgtt gtaggcgcct cgcaacttga aggaaaacac gggctgcatg
     2341 tcttcgcgct tcagcagcaa gcaattgccg attcgtgcgg agaggttggg tgcatgatcc
     2401 agcggcgact cgacggcgac atcgtagacc tgcgcattga ggattttttc gagatagtca
     2461 gggtgcatgg cagtgaaagc tattgtccgg ggccgctgat tctaacagca gactcctgac
     2521 ggcgtgcggc gcagcccgcc gtgcgctgcc aagcgacatc ccgccgatct gggctactat
     2581 ttcccttttg caagagtccc acggacctgc catgatcgcc cgcctgcgcg aaatccccta
     2641 caactacaca tctttctccg accgggaaat cgtcatccgc ctgctgggtg aggaaaactg
     2701 ggcgatcctc gacgaactgc gcagccagcg ggtcaccgga cgctcggcgc gaatgctgta
     2761 cgaggttctc ggcgatatct gggtcgttca gcgcaacccc tatctcgaag acgacctgct
     2821 cgccaaccgc cagcgccgag ctgcgctgat ccatgccctg cgtcaccgtt tgaacgagat
     2881 cgacaagcgt cggcaaggta acgcgctggt actcaaactg catgactcgg cgcgagccgc
     2941 ggtcgatgcc tttgaacgcc attttgacga caccgccacc ttgcgccgcc gggtgttgaa
     3001 aaccttgctc cggcataccc ggcgcgacaa catcgccttc gatgggctgg cgcgcgtctc
     3061 gcatgtcacc gacgccaccg actggcgcgt cgaatacccg tttgttgttc tctaccccga
     3121 cagcgaagag gaattggcgc cattggtgtg cggctgcatc gaactcggac tgaccatcat
     3181 cccgcgtggc ggcggcactg gctacacggg cagcgccgtt ccgttgacgc ccctgtcggc
     3241 ggtcatcaac actgaaaagc tcatcgatat cgggccagtt gaagaagcaa ttctgcccgg
     3301 ctgcgagcag ccctgcacca ccatccgaac cggtgcgggt gtcgtgaccg cgcgcgtttc
     3361 tgatgctgct gccgccgccg gcaggatatt tgccgtcgac ccgacctcgg ccgaagcctc
     3421 atgcatcggc ggcaacgtcg cgatgaatgc cggtggcaag aaagctgtgt tatggggtac
     3481 ggccctagac aatttggcat ggtggaagat ggtcgatccc gacggcaacg tgatggaagt
     3541 cagccgcatc gatcataacc tcggcaagat tcacgagcag gccgtcgtcc gtttcgaaat
     3601 ccgccgcttt cgaatggatg gaacaacccc gtacggggaa cccgaactgc ttgaaataac
     3661 cggccaaacc tttcgcaagg cgggccttgg caaggacgtc accgacaagt ttcttgcagg
     3721 tttgccgggc atacaaaagg aaggcgccga cgggctgatc gtcgcggcac gctgggtgct
     3781 gcacaaaatg ccgccgcatg cacgcaccgt ctgtcttgag ttcttcggac aggtccgcga
     3841 ggcggttccg gccatcgtcg agatcgccga ttacttcaag ccgggaggtg ccggccatcg
     3901 gaatggtgtg ctgttggctg gactggaaca cctcgacgaa cgctatgtga gagcggtcgg
     3961 ctatgcgacc aaggcaaaac gcaaaggcga aggttccggt cgtctgaaaa tggtattgat
     4021 cggcgacctg gtcggccacg acgaaaaggc ggtcatggcc gctgcctcgg acatcgtgcg
     4081 actctgcaat ctgcgcgggg ccgagggctt catcgccgtc gacgccgata cgcgcaggag
     4141 attctggctg gaccgttcgc gcacagccgc catttcacgc cataccaacg ccttcaagct
     4201 caacgaggac gtggtcattc cattgccgcg catgggcgac tactgcgacg ggatcgagca
     4261 tatcaacatc gaactgtcga tacggaacaa actggaattg tgcgacaagc tggccgattt
     4321 cctctccggc acactgccgc tcaaccgtgg cgacgccaac atcgcgagtg acgtactgat
     4381 tggcgatcgc aggcaaaggg caatggatgc tgtagggata gtccgggcgc gctggcaatg
     4441 gcttctcgat catctcgatc aaccgttgga gcagatggag gcgcagtttt cttcattcgg
     4501 catcttcgcc ggaacactct ggtgcaaaga agagaaccct gtgcttttcc accgccttca
     4561 ggactactcg atacgtgtgt cgtggaaagc ggaactgaaa ccgcagctcg aagccatctt
     4621 cgtcggcaat gtctttcgcc cgatactcga acggatcggg gcgatccacg cggaaaccct
     4681 gcgcggccgc ctgttcgtcg cgctgcacat gcatgccggc gacggcaatg tgcacaccaa
     4741 catcccggtc aattcggaca attatgcgat gttgcaaacg gcctatgcga cggtagagcg
     4801 catcatggca ctggctcgtt cgcttgacgg cgttatttcc ggagaacacg gcatcggcat
     4861 caccaagctg gagttcctga gcgaagaaga gatacagcca ttccgcgatt acaaatccag
     4921 ggttgacccc aaggggcact tcaacaaggg caaactgctg cccggtggcg atctggcgaa
     4981 ggcctacacg ccgtccttct ctctgcttgg tgcggaatcg ctgatcatgg agcaatccga
     5041 gatcggcaat atctcggaga tggtcaagga ctgcctgcgc tgcggcaaat gcaagccggt
     5101 ctgttcaacc cacgtaccgc gcgccaatct cctttactcg ccgcgcaaca agatcctcgc
     5161 cacttcactg ctgatcgaag catttctcta tgaagagcaa actcggcgcg gcatttcgct
     5221 gatgcacttt gatgaattca acgatgtggc ggaccattgc acgatctgtc acaagtgcgt
     5281 agcgccctgc ccggtcgata tcgattttgg cgatgtctcc gtgcgcatgc gtaatttcct
     5341 gcgcgaacag ggcaagaaga aattcgttcc cgccaaggca gcggcgatgg cctttctgac
     5401 catgaaggat gctgcgacca tccggctgac gcgcaagctc atgatcgact ggggctaccg
     5461 tgcgcaacgg atggctcacc aggcagccaa agcccttggt ctgattcagg atcagacccg
     5521 gcagcctccg tcaacacttg gccagccgac ggtacgcgcg cagatcatcc acttcatcaa
     5581 caaaccgatg cccggcggcc tgcccaagcg cacgtcgcgc gccctgctcg acattgagga
     5641 cgaaagaatc gttccggtaa ttcgcgaccc gcacaaggtc aatgcagccg actactccgg
     5701 cgacgccgtt ttctatttcc ctggctgtgg ctcggagcgt ctcttttcgc aggtcggtct
     5761 tgccacacag gcaatgctct acgaaatcgg tgcgcaaacc gtgctgccgc cgggctatct
     5821 ttgctgcggc tatccgcaga atgcagccgg cgaggcggat caagggcaaa gaatcattac
     5881 cgacaaccgc gtgctctttc accgcgtcgc caatacgctg aactatctcg acatcaacac
     5941 ggtgatcgtt tcctgtggca cgtgcatgga tcagttgcag aagtaccagt tcgaaaagat
     6001 atttcccggt tgccgactgc tcgacattca cgaatatctg ttggagaagg gtgtcagtct
     6061 tgagggagta agcggcaccc gctacctgta ccacgagccc tgtcacgagc cgatgaagac
     6121 catggcggga atcaaagtgg tcaacgaatt gatggggcag aacgtcgatc tctcagatcg
     6181 ctgttgcggc gaatccggca cgctgtccgt gacacgcccg gacgtatcca cccagatacg
     6241 cttccgcaag caacaagaaa tcgagaaggg agtggccaag ctgcgcgccg aggggggaat
     6301 gggtgcaagc ggcagtacta catcgtttac gggcgatatc aagatcctca cctcctgccc
     6361 ttcgtgcctc cagggattgt cgcgctacaa cgacgatggc ggaacgcagg cagactacat
     6421 cgtcgtcgaa atggcgcggc accttctcgg cgcggactgg atggagaaat acattcaaaa
     6481 agcaagcaac ggcggcattg agcgcgtcct gctttagtcg atattcaggc atgatttgat
     6541 cgaaaggctc aggcaccgca ttcagcagag ccggttcggc aaaaggcagc cctgggccgc
     6601 gattgacggc atcatgctaa actcgatcaa catacgttta gcgcgattcg ctgcccatag
     6661 tccattgcct tctttgccaa ctttttctcc tcctttacca cgtaccgtac gggtgcgcaa
     6721 agccgtcgag gcgggtgcaa cagccacccc ttccgcttct cgctcagcaa aaccgcttcc
     6781 gcattcgctg gctcccgatt cgctgggctg gagcctgctg ctggccgcgc gcgtcttggc
     6841 cgcagttctc ggcgggaagg gtttgaacga cgccctgcgc atgcttgccg acgagcctcc
     6901 gaaagctcgc gcggcagcgc aggatgttgc ctatggcgtg ttgcgccgtt acggcttcgg
     6961 cgaattcatc cttggacaat tgatggccaa gcctctgacg catccagaaa cgcaagcgct
     7021 gttgctggga gcactttatc gcctggagac gcgcgccgat tcaacgccga tgatcgttga
     7081 tcaggcggtt gccgcggccg gcgaactagc ggggggcgtt ttcaagagtc tggtcaatgg
     7141 cgtgctgcgc aattacctgc ggcagcgcaa agcgcttctc gctgcgatgg cagataacga
     7201 cgaagcgaga catcagcacc cacgctggtg ggtggctcga ttgcgccgtg cctatcgtga
     7261 caattggcag tcgatcgttg ccgccggcaa ctgccctccg ccgatgactc tgcgcgtcaa
     7321 tcagcgtcgg acaaccgtgc cggactaccg cgccagcttg gaagcggcag gtttcgcagc
     7381 acgaccggtc ggtcgccatg gtttgatcct ggaacgaccg gttgcagttg annnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtggtgcgt cgtcaccccg atatcaagac
     7561 actgcgccgc gagagcgata tccggcgttt tgcgcttaag caggcagcca ttctcgatgc
     7621 gctctggcct ttgctcaaac ccggcggtaa gctgctgtat gcgacctgct cggttttccc
     7681 cgaagagaat gctgcgcaga ttgatgcatt tctcgttcgc cattccggca tcgagcgctt
     7741 gtttgaagag caactgctgc ctcaggacga aaacgacggt ttctactatg cgttgttgcg
     7801 aaaaactgtt tagagcaatt ttctgcagcg ccgctctgct gctgaccgcg cttacaacac
     7861 aggctgcgga gatcagcatc cgcaacccgc agctcgaagc aaacgaggac ggttacgcgc
     7921 tttccgccga catgaacatc aacttcaata cgcggcttga ggaagctgtg aacagaggaa
     7981 tcgtactttt ttttgccgcg gatttcgaac tgacccggtc gcgctggtac tggttggacg
     8041 aacaggtcgt ccggcgcagc aagatattcc ggctttccta tcacgcactg acgcgtcaat
     8101 atcgattgtc caccggcgcc agcgccctgc accagagtta cgcttcattg gatgaggcac
     8161 tgagcgtgat gtcgcacgta cgcaattggc aggtgctcga gaaggatgag gtcaagccag
     8221 gccaagccta tctcgccagc ctgcgcatgc gcctggatct gtcgctcatg cccaaaacct
     8281 ttcaggtgag tgcactggcc aacagggatt ggaatctttc atccgagtgg ttgcgctggg
     8341 acttcatgcc ttcggaattg aagcaggagc cgccaccggc gatatcgaca ccggcgacac
     8401 cttcggcgaa ccctcactcg acacctgcgt cgggcacgcc ccccgcttcg cctgcaacgc
     8461 ccgccgcggg tgaagccaaa tgaagaacct gatcatcgta gcggcttcgt tcggcggcat
     8521 cctgctcttt ctgctggcgt caaccagcgc caatacggca atgtttgcca gcaactatcc
     8581 ctggctactt ggactgaacg cgtttgtcgc cctgtcgctc cttgtcctga tcatttggca
     8641 ggtacgcgat ctgtggcgcg aacaccaggc ccggattttc ggttcgcggc tcaaactgcg
     8701 cctgatgctg atgttcggcc tcatggccgt cctgccgggc gtgctgatct acgccgtttc
     8761 ggtgcagttc gtcaccaaga gcatcgattc ctggttcgac gtccgcgttg aaaaggcact
     8821 ggagtccggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc caagagcatc
     8941 gattcctggt tcgacgtccg cgttgaaaag gcactggagt ccgggcttga tctcgggcgc
     9001 agtgcgcttg attccctgct cgacgatctc gccgaaaagg ggcgcgtgat gtcgcttgag
     9061 ctgggcaatc agtccgaaat ccagcaccaa ctggcacttg gtcgcttgcg cgaacagact
     9121 ggcgtgcagt ttgccgccct cttttcaacc aacggacaac ttctggcaac tgcgaccagc
     9181 gatctgggga ttaccatgcc aatgcagccg tcgctcgaac aattgcgaaa ggcccggttc
     9241 ggacgaggct accgagagat cgaaagcgcc ggcggcacgg agctgatgct gcgcgttctg
     9301 gttgccgtat cccccatggg cctgggtatg gaaacgcgca tcctgcagtt gacccagccg
     9361 gttccatccg gtcttgcacg caacgctgat cgcgtgcagg aggtctaccg cgactatcag
     9421 gaactttcac tgggtcgcca ggggttgacg cgcatctatg cgatgacact gaccttgacc
     9481 gttctgctgg ccttgttcac ggccatcgcc atggccttta tcctggctcg ccgcctttcg
     9541 gcgccgcttt caatcctcgc cgagggaacc caagccgtgg cagcgggaga ctttacccca
     9601 cgtcaggcga tcaacagccg cgacgagctc ggtgtgctga cccagtcctt cagtcagatg
     9661 acgcgtcagc ttgacgaagc acggcgtgat accgagcgtc atcgcagtgc cgtcgaatcg
     9721 gcgcgcgcct accttgaatc catattggcc aatttgtcgg caggagtact tgtatttgac
     9781 agcaacttca tcctgcgcac ggtcaacgag ggtgcgaaga caattcttga tgatcagttc
     9841 gatgacctcc tggccgtgac cgtcgacgcc tggccgcgcg agcaggttct cggtggaacg
     9901 atccgcgatg cgtttgccga gtacggacgc aaggaatggc atacccagat cgaacttgat
     9961 cgaccgggca gtctgccgca aatcctgctg ttgcgtggaa cgcaactgcc ggaagggacc
    10021 aacggcggat atgttgtcgt attcgacgat gtcactcgac tggtggccgc tcagcgcagc
    10081 gccgcctggg gagaagtcgc gcgacgcctg gcacacgaga tcaagaaccc cctgacaccg
    10141 atccagcttt cagccgaacg aatgcagctc aagctggcca gcaagttgag cgccggcgat
    10201 gccgaaatgc tcgaccgctc gacgcagatg atcatcaatc aggtgcaggc gatgaaacgc
    10261 atggtcaatg agttttccgc ctatgcccgc atgccggcac ccgagctggg ccgtcttgac
    10321 ctgaatgccc tgatcagcga attgcttggt ctgtacgaga gctcgcaaaa tcggatcaat
    10381 attgagcttg acgctgtttt gcccgccgta tggggcgacg cgagccagtt gcggcagatt
    10441 attcacaatc tcctgcgcaa cgcccaggat gctcaggagg gcgttgaggc agcgcagatc
    10501 ggcgtgacta cgcgcgtcgt cgaaggaatg gcggaattgc tcgtcaccga ttgcggccca
    10561 ggcttcccac ccgaaatcat ggcgcgtgct ttcgagcctt atgtcacaag caaagcggga
    10621 ggtaccgggc tggggctggc catcgtcaag aagattgtcg atgagcatca gggacacatc
    10681 aggataaaga accgtcagcc gacaggcgcc gaggtgagca ttcgtttacc cttggccgag
    10741 cgactgacgc ccaatcccat tacaacaact atgactgaaa caactgacga aagctgatca
    10801 tggcgcagat actggtggtt gacgacgaaa tgggcatccg cgagttgctc tcggaaattc
    10861 ttgccgacga agggcatacg gtcctgctcg ccgaaaatgc cgcagcggca cgtaacagtc
    10921 gtagcgaaaa gcgtccggat cttgttctcc tcgatatctg gatgccggat acggatggca
    10981 tatcgctgct caaggagtgg tcgtcagccg gtttgttgac catgccggtt gtcatgatgt
    11041 ccggccacgg aacgatcgac tccgcagttg aagcaacacg cgtcggcgcg atcgattttc
    11101 tcgaaaagcc gattgcattg cagaagctgc tggccaccgt caagaaagcg ctcaaacacg
    11161 atgtttcgcc gcagttgccg ccccttacgc tcgatgcctt tgcccgctcg ccgctgctca
    11221 aggatttgaa gaaacgactc gaacagaccg cagccaagac ttctctcctg ctcctcaaga
    11281 atgcctccgg cagcattgcc gagatctgcg cacgtaccct gcacacgccc aagacgccct
    11341 ggcttgatct atctgcgtcc agcgaacccc tgacccagga gatgctagca aatgcagcgg
    11401 gcggcattct gtttcttgct gacctgagcg tgctgggaaa gctgcagcaa aagaatctcg
    11461 tttatgcact tgagcgactc gagaaataca atctccagct ggttgccgcc tcggcgcgcc
    11521 cgctatcttc gctgatcgag aacggctggg acccggcgct ggtaacgcgc ctgagcgaag
    11581 tctgggtgtc tttgccgcaa ctttccatgc atacggatga cgttccggaa atttcgacgc
    11641 tggttctgtc gcacctcatt gcacgcaacg aggtgccgtc gagaagtttt tccagcggtg
    11701 ccctcaacgc actgcgcctg catcactggc agggcgaatg ggccgagttg ctggcgacga
    11761 tcaagaatct cgctctctcg gcgctggatg acgatatttc ggtcgatgac gtagatagca
    11821 ttctgcttcg cgaggcgcgc gatgcacccg aagcgccacc ggcgctaccc ctgttcaacc
    11881 agcccttgcg cgaagcgcgt gaagctttcg aacgcctcta cttcgagcat tatcttaaaa
    11941 gcgagggggg aaacatggca cgtgtcgcag acagaaccgg ccttgagcgt acgcatctgt
    12001 atcgcaagtt gaagcaactc ggattgaacc tcggccgacg cggcgaagac accgaatgaa
    12061 gcctcccaac attagttcaa ggcgggcgcg atcatgagaa ttgtcatcct cggcgccggt
    12121 caggtcggcg ccagcattgc ccagattctc gtctccgaga ataacgacat cacaatcgtc
    12181 gataacgatc agaagcggct cgagtatctg caggtgcgtc tcgatttgcg tacgattgcc
    12241 ggaaatgcag ttctgccatc cacactgcgg gatgccggac tggccgacgc cgacatgttg
    12301 attgcggtca cgcgaagcga ccagaccaac ctcatcgcct gcaaactggc gcacacgatg
    12361 ttcaacgtgc cgacgcggat cgcacggctc cgctcgtccg aattcatgga cgatgctgag
    12421 ctgctgaccg agaagaattt cgcggtcagt tatgcgctgt gcccggagca ggtgatcacc
    12481 gactacctgg tcaagctggt tgagtttccc gaagcgctgc aggtgcttta ctttgccaat
    12541 gagcgcgtcg ccctggttgc cgtgaaagca accgccgggg gattgatggt gggcaagccg
    12601 atcagggaaa tgagcgagca cctgggcgga aatgtcgatg ctcgaatctc ggccatcttt
    12661 cgacgcgacc atgccgtcac accaaccggg agtacggtca tcgaagaagg cgacgaagtc
    12721 ttcgtgctgg ctgctgacga gcacattcgg cgggtgctga tcgaaatgcg acaaaaaacg
    12781 actccggtgc agcgcgtcat cattgccggc ggcgggaaca tcggcttgcg tgttgcgcaa
    12841 atgcttgagg gaaagtgtct ggtaaaggtc atcgaaagca atacggcccg cacgcagacg
    12901 atcgccaacc tgctcggcaa cggcctggtg cttctcggcg atgcgactga cgaggagttg
    12961 ctggaacagg agtcgatcgg cgaaaccgat ctttttctcg ccctgaccaa cgacgaagaa
    13021 gacaacatca tgtcggcctc gctggccaag gcaatgggtt gcaagcgggt gctggcgctg
    13081 atcaaccggc gagcctatgc cgacctggtg cagagcggtc cgatcgacat cgcaatctct
    13141 ccggcgctgg tctcgatcgg agacctgctc acccacgttc gccggggctt cgtggccaag
    13201 gtgcacagtc tgcggcgtgg cgcggccgaa gccattgaac tggtcgttca tggcgatgaa
    13261 aagagctcgc gggtcgtggg tcgtcgggtt ggcgatctgc cggtaattcc cggtgcgacg
    13321 ctcgtcgcga tcgtacgtga cttcgacagg attgaagata tcgcttacgc gggcatgctc
    13381 aagataaaga agaaggggca tgtggttatc gcccacaagg gcgaggtcat caagcccgcc
    13441 gatcacgtca tcgtattctg cttgaatgcg aaagtggtca agaaggttga gaagcttttc
    13501 tcggtcggac tgcatttttt ctgaacgcat tccgatgaaa gtttacttac cggtcattcg
    13561 tgttctgtcc atggtgatct tcattttcgg cttgaccatg ctggtgccgg taggtgtttc
    13621 atgggttttt caggacggcg cggcgcgcgc gttcgacgaa gccatcctgc tgaccctggg
    13681 cgtgggactc gttctgtggg gtttcacgcg caggaacaag cgtgacttga cgatccgtga
    13741 cggcttcctg atggtcgcgc tggtgtggac ggtgttgccg gtgttcgctg cgctgccact
    13801 cattttttat cttgacgcca gttttaccga tgcctacttt gaagccgtat cggggttgac
    13861 aacaaccggg gcgacggtct tctcggaatt ggacaagctg ccggtgtcga tcaatttctg
    13921 gcgcacccag ctcgtctggc ttggcggcat gggtttgatc gttctggcgg tagcgatact
    13981 cccgctgctc ggcattggtg gtcgtcagat gttcaaggcc gagacgccgg ggccaatgaa
    14041 ggattcgaaa atgaccccgc gtatcgccga aacggccaag ggtctgtggg gcgtctatac
    14101 cggcataacg gtgtgctgct tcctcgcgta ccactgggcc ggcatgagtt ggatggatgc
    14161 catcatgcat tccttctcga ccatgggcct gggcggattt tcgagccacg atgcgagttt
    14221 cggctacttc aactcgccaa ccatcgaaac ggtcagcatc gtcttcatgc tgattgccgg
    14281 catgaatttc gcgacgcact tcgttgcgct ttccggccgc tccatccgcc cgtacatcac
    14341 cgatcccgaa gccgtatggt ttctcgtcgt ggccctcggc agcgttctcg gaatcgccgt
    14401 gttcctcaat gagaacgacg tctatcgcag tttctctgaa gccttccgct attcggcatt
    14461 caacgtcgtt tcgatcgcca cgaccacagg atatgccaac accgactact acctctggcc
    14521 gatgttcgca ccgctgtgga tgctgtttct ttccagcttt gcaaccagtg cggggtcgac
    14581 cggcggaggc atcaagatga tccgcgccct tgtcctgtac aagcaggtca accgcgagct
    14641 gttgcgcgcc atccaccccg atgctgtcta tctggtgcga atcggctcgg aggtggtacc
    14701 cggcaacatt ctttttgccg tactcgcctt tggcttcatg tacatggtca gcattgtctc
    14761 gatgaccctg ctgatgtccc tgtcggggct ggatatcatc accgcctttt cggcggtcgt
    14821 agccagcatc aacaacaccg ggccgggact taatcaagtg gggccggcaa cgaccttcga
    14881 ggtgctcaac gatttccaga cctggatatg taccttcgcc atgctgctcg ggcgtctgga
    14941 aattttcacg ctgctggtcg tctttacccc ggccttctgg cgccgctagc ctgggtacgg
    15001 cgggcgtcgg tagcgctggc cctgcgaacg cgggataatc gacctttctc gactccgccc
    15061 gtcgctctcc acgctgatac ggattcaggc catttacctc ctcttcaaca aagcctaacg
    15121 tgacccgacc ccgcaacgac aatttcctgc gcgcgctgct caaggaaccc accgagtaca
    15181 ctccgatctg gctcatgcgt caggcggggc gttacctgcc cgaatactgc gaagcgcgca
    15241 aacgcgcggg cagcttcctg caactctgca agaatccgtc cctggcctgc gaagtcacct
    15301 tgcagccgct cgcccgctac gaccttgatg cagcgatcct tttctcggac atcctcaccg
    15361 tgcccgacgc catgggcctc gggctatatt tcaccgaagg cgaaggaccc agattcgaac
    15421 gcccgctgcg ccaggaatgg gaaatacgtg acctgaccgc tcccgatcct tacgagcacc
    15481 tgcgctacgt aacggacggt gtttcagaaa tccgccgtgc gctggataac tcggtacctc
    15541 ttatcggatt ctccggcagc ccttacaccc tggcctgtta catgatcgaa ggcgcgtcga
    15601 gcagcgatta tcgacacgtc aagaccatgc tctatgaccg gcccgatctg atgcatcgca
    15661 tcttgtcggt gacggctgat gccgtgattg cctacctgaa tgcccagatc gaatccggtg
    15721 cacaggcggt gatgatcttc gactcatggg gaggaggtct ctcggctgcg gcgtatcccg
    15781 agttttccct gcagtacatg cggcgcatcg tctccggttt gatcaaggag aaagacggcg
    15841 aacgcgttcc ctcgatcata ttcaccaaag gcggtgggtt gtggctggaa tccatcgccg
    15901 atagcggttg cgatgcagtc ggactcgatt ggaccatcga cattggcgag gcgcgtcgac
    15961 gcgtcggtca caaggtcgcc ctgcaaggca atctcgatcc caacgtgctc tttgcttcac
    16021 ctgacaagat tgccgacgaa gcgcaaaaag tcctcgacag ttacggttcc ggcgataccg
    16081 gccacgtctt taacctcggg cacggcattt cgcaattcac ccagccggaa cgtgtaacag
    16141 cactcgtcga aaaggtacac agctacagca aggttttgag agaaaaaaca tgaacttttt
    16201 tcatcattcg ccttgactta tgcacagaaa ctgattcgct gtcacagaac ctacacaagt
    16261 cctttcaaaa tcagtgactt attcttatct atttgattaa taacagtaaa ttgtgtgcct
    16321 tattttgcgg caaagtaagc aaccgccttg ctctcagggt gtttgaaggg gttttggtca
    16381 actacttcac agacttatcc acagaaaatg tgcacaagtg agaatacatc gattagttaa
    16441 tgagttacgc ggctattgca gaaccggcgc gaactttccc agctaagcga agctgactac
    16501 ggttagcgtt tggtaatttc tggcgtattt aacgaatagg cgtcggaaaa agtacagatt
    16561 cttactgacg ggccgcaaac atccggcggg ccaacagggg acaaatcggt gtttccggac
    16621 tcatggactg gtcagtatgc agatagctcg cgtcgccctt gatgttccgc tgcaccggtt
    16681 ctttgattac atcgttcccg agaccgaggc gttgtctgat tccgacctcg gtcggctggt
    16741 ccgcgtcccc tttggccaga aatcgaggat cggcattatc gtcgcgcttc cgcagtccag
    16801 tgacgtcccg gtcgcccgac tcaaatcgat cgaagccgtg ctgcgcgaca ttcccgcact
    16861 accggcagac tggttccgcc tgagcgagtt ctgtgccgct tactatcaag ccccgctcgg
    16921 cgaggtgatg ctatcaactc tgcccgccgg actgcggcgc gtcgccccgc ccaaggcccg
    16981 aacgagccag cgttcagtaa aaccgtcacc gccgcaggaa ccgcccgaac tgacggctga
    17041 gcagaagaca gcgctggcgg cgatagccga aggcgaccaa ggattcaaag cctatctgct
    17101 gcacggcgtg accggcagcg gcaagaccga gatctacctg cggctggtcg agcggtcgct
    17161 ggcagccggt cggcaggtgc tgatgctcgt tcccgaaatc aacttgacac cacagcttga
    17221 atcccgtgtc gctgcacgct tcccgagtgc cgggctggtc agtctgcaca gcgagttgac
    17281 ggaggcggcg cgcacgcgca actggcgcgc agcgctggaa ggacgcgcgc gcattgtgct
    17341 cggtacgcgc ctggccatct ttgccccgct gccagacctt ggcctgatcg ttgtcgatga
    17401 agagcacgac gcatccttca agcaacagga cggaatgcgt tactcggcac gcgacgttgc
    17461 cgtctttcgt gcccgtgaac gaggcattcc gattgtgctc ggatcggcca ccccatcgct
    17521 ggagagctgg gcgaatgcca ccgaccaccg gacgcccgct cgctacacgc tgcttacact
    17581 gcaccagcgg gccgttgaaa attcacgcct gccgcttgtt caacgtatcg atacccgccc
    17641 ggaaaaactg caggacgggc tttccgcagc gctgctgctg gggattgaaa agcgcctgac
    17701 acgcggcgaa cagagcctgg tttttctcaa tcgtcgcggc tacgcgccgg tcctcgcctg
    17761 caccaactgc ggatggatct cgcactgcca tcgctgcgcc gccaatctcg tcttgcatct
    17821 tctcgacagg cgcctgcgct gccatcattg cggcttcgag agtccgatac cgaaagcctg
    17881 cccgacttgc ggcaatcagg acatccagcc tttcggccgc ggcacccagc ggctggaatc
    17941 ggtgctcgcc gagcgcttcc cacaggcgcg catcctgcgc gtcgaccgcg attcggcccg
    18001 cagccgcaag cagtgggaag cgctggtcca acggatttac agcggcgacg ccgacattct
    18061 ggtcggtacg cagatgctgg ccaaggggca cgatttcccg aaactgacac tggttggagc
    18121 gctcggcgcc gacgcagcgc tgttcgcttc cgactggcgc gcgccagagc ggctgtttgc
    18181 ccagttgatg caggtggccg gtcgcgccgg ccgcgccgaa ttgccgggcg aagtgctgat
    18241 ccagacacag tttcccgacc acccgcttta cgccgcactg gtccagcacg actacgcggc
    18301 gttcgccgac agccaactca aggagcgcga acaggccggg ttcccgccct ataccttcca
    18361 ggccatgctg cgcgccgaat caccccacat ggccgacgcg ctggcatttc ttgcgacggc
    18421 gcgggccggg ccgcagactg gcgcgcatgt cgaagtgatg ctctacgatc cggtgccgat
    18481 gcgcctggcg cgtcgcgcca acttcgagcg tgcccagttg ctcatcgagt cgccgtcacg
    18541 acgcgccctg caggccttcc tcggtgcatg gcgactagtg cttgagacga tcaagactcc
    18601 gtcgcgtctg cgctggcatc tggaagtgga cccgctggag ttctgagcaa agccatgcaa
    18661 agtcaacgtg aacggcgact cgaaacgcag gcgggtagcg tcaaagcgta atccagagcg
    18721 tcctgaaacg ccgttgtccg accggcaaat ccttacacat tagcgaccct ttctcgcgcg
    18781 tgccgtcatc cgagaacacc agcatcctga ccggggggcc ggggtaggca atcatggctt
    18841 ccggttgcag gccgctgaaa ttttctccat tcactgccac cgggtcctcg tctgctacgc
    18901 cagaccaccg gtacatttgg aatttgccgc taacgcccgc cgcgccggcc atgatgatgt
    18961 actggcccag tgcgggcacg taatcaatgc tgcgcacgcc acgcccgccg agagccaaca
    19021 ggatcggcgc acccagccgg gccgacttgc cggcaacgac ttcctgtgga ttattcagcg
    19081 gaatcagcag cgccttgcca tcgggaatgg ggccgcgcag accgatcagc aagcgcccat
    19141 ccggcgtcgc ggcaagtccc tcgatgttca acccgcctgg ctccttgggc ggaatctttg
    19201 ccgcatcgcc cagcttgtag gctttgagcg caggcaaccg ggtcaagtcg cgcaccagct
    19261 tccgataggg tttgccgacg acagacagct caacggcttc accggctcca ttgatctggg
    19321 tcgcaaaaaa ccgccgcctt ccactgcgca acttgccatc gctattggtg ccgtgcgaag
    19381 cgatccagta aatccggttg ccgatgcgag ccgccgcttc aaggtcggcc tcggggtgtt
    19441 tcgggtccga cttcagaaat tccgagagat cgagtgtgct gacgggtgcc gcggtccggc
    19501 cctcccgata aacgcgcagt acattgtcct catcattggc aacgacaaat tgctgctcgc
    19561 ccagcgagac cgccgccgaa acgtcacagg taccgccata ctcggtcgct gtagcgaacg
    19621 cccgggattc aatggtcacg aacgcgccaa ccaatgcgca gagcagcatc ggcaccgccc
    19681 ggacttttct catggatcgc acgtgccaca aggcaaattg cgcccgctga acagcgcgca
    19741 aacaagtggt cagttccatt ggtttgctcc tcttgattct gctggggtca attacagcgg
    19801 cgcgaagccg gattcaaggc ggtgcggcgg tacgtgcccg gacacccgcc agccgggtca
    19861 gcagccctct tgccaatccg cccagaacga acaaccatac cgcaaaggcc acccagatca
    19921 tgatctgcga aatccattgc agcggcggaa actcggcagc cagaccgaga cgggcactgg
    19981 cgacggtgta catgcccagc gggaagacga agctccacat cgccggctcg tatttaagcg
    20041 gaaggccgcg cgcgacatgc ttccaaaagc cgaacatcaa gagcatggga atccaccagg
    20101 tgccccatgc ccatatcatc atcgtgacgc cttcgacgaa aggtctctgc gcggcaagga
    20161 atggcaggta cggatcctcg ctgagcagag aggtgccggc attggcgctg atcgccgcgg
    20221 cgcccatgat gacccacagc agcggcgaga tatcctgcgg cttgagcgcc aggaaaaaga
    20281 tgcgatagca gaagagggtt acgaaaatgc catagaacag gagaccgagc ccccacagca
    20341 tatgcacttc gaccatcatg tagccggcgt actcgcccag tgcgggcgca atcgccgcgc
    20401 ccagcaagac cagcgactgc gttcccacga tggagatcaa ccagccgccg tgcacgatgt
    20461 tgacgttgtt ctcgtggctc aagaaggtca gcacgctgaa ggcaagatag agcagcaagc
    20521 accaggcgag aaaagcgatt acccagcatc ccatcgccag accggtatga ccgcgcgcgt
    20581 tcaacagcag tccgacaata tctgtcgcag ccaccagcgt gaagaatgaa aaaaccattc
    20641 gcggattgag caggtcgatg cgcaccgctc gcgaaaacat gaaaaaccgc cccacgttca
    20701 gcgtcagtaa cgtcacccag gcaacaatcg cgaagacgca aacaacgtcg gccagcaatt
    20761 catggtgcag cgtactgaaa cctatcgaga tgatcccgct ggccatgacc agcgcgaagt
    20821 ttcccggatg caacgcctcc agcgagaatc ccggcgctgg tgctggtgct gactgcgagg
    20881 tgtcggtggg tggctttggc atggactgaa cgggctgaag ggtggctagt cgggacagga
    20941 agaagcatca ctgcccgcgt ggtttgcaga gatagtagcg cgatttgcac cgcgccagtc
    21001 agtctggcct tgccgctttg aaacaacatc agccgatgtc atccgcgttt ccacaagcga
    21061 atgacgtccc ggcacatggc taccggttgc cggaaaccag ctcgccacgg cgccgggcgc
    21121 gtgccgccat ttcgtggtcg ccggtcttgt ccgcaagctg cgcctgaacg cccagccagg
    21181 ccagcaaggg ccgacgccaa ccctgtgccg atgcggtgtc taccgcgaga tcgatatccg
    21241 ccggggccag tcggccgctt cggagcagca ccccggcggc aatcagtcgt ggcagcggcg
    21301 cctcgatcga atcgagcgcc gcttgccccc cgtcgagcac agcccggtaa tgctgcggca
    21361 gcagggcggg gtcggctggc tggccgttga ggaatgcagc g
//
LOCUS       DAORPM010000008        20823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_431_length_20823_cov_11.735073, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000008 DAORPM010000000
VERSION     DAORPM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 20823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20823
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_431_length_20823_cov_11.735073"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..269
                     /locus_tag="PLD53_00805"
     CDS             18..269
                     /locus_tag="PLD53_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRC59471.1"
                     /translation="MQVEHHELHREFPEYLEVMQALRKTDSSFSKIFDEYDSLTSEVE
                     RLEEEDIPVDDFTIEEMKKVRVLLKDKVYQILVAHKDSN"
     gene            308..1480
                     /locus_tag="PLD53_00810"
     CDS             308..1480
                     /locus_tag="PLD53_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC59472.1"
                     /translation="MHRIPYWRLSGFYFFYFAFIGVFLPYFGLYLQTLSFSAWDISVL
                     MSQMQLMRLFGPYLWGTLADRFVSRVPIIRLAGLIALMASASFFHIRSFEAILVAMAV
                     LSFFWSATLPLVETLTFEHLRENPERYSRIRIWGSIGFIVAVMGAGALLDHVKLPSML
                     WMIVFALAGTLLYAIVIPEAEPALHHADHPPVLEILRQRRVVALFSACFSMSAAHGAL
                     YVFYSIFLSDHGYSKSVVGGLWSLGVLAEIAVFFYMSRLLRVFGLRTILLLSFSAAVV
                     RFMMIGWGVEQPFVLVFAQVLHGLTFGSFHAAAITVVNRWFLGRTRSRGQALYSSLTF
                     GAGGLVGGLISGWTWDHLGGELTFALSSIFALIGLILVMVWIRETDVATHQVPYPV"
     gene            1611..>2863
                     /locus_tag="PLD53_00815"
     CDS             1611..>2863
                     /locus_tag="PLD53_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59473.1"
                     /translation="MLKKFRSRLRKLLHVSIRKLISWLPRNQRFAVFRSFADCNPAPS
                     KRLVLKIAETKEELEACFSVLHDAYVDSGFMKPDPSGMRVTIYHALPTTTTLCAKYDG
                     EVVGTISLVRESALGVPLQTIFDLTPLRQKEGFIAEVSALAVHRKFRKTGGSILFPLM
                     KFMYEYCTTFFDTRHLVIAVNPSHIEMYESLLFFKRLTATMVEKYDFVNGAPAIGATV
                     DLKEVLDTFREHYASKPPHRNLYAYFTQVKMPNIQFPSRRFFTTNDPVLTPELLDHFF
                     NVQTKTFENLNERQKALLHSIYDLPAYWKILPALPDSAEVIPIRRHQRFSVRCPARIV
                     VATSDERQEIVMEVVEVSRYGLQARARDPLPLNTWCDLTVQVGRSDVAHFRALALREN
                     ENGFNGFYGFRLGEPDVVWQKFVSAL"
     assembly_gap    2864..2963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3054..5483
                     /gene="nirB"
                     /locus_tag="PLD53_00820"
     CDS             3054..5483
                     /gene="nirB"
                     /locus_tag="PLD53_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HRC59474.1"
                     /translation="MSKPKLVMVGNGMAGVRTLEELLKISPDHYDITVFGAEPHPNYN
                     RILLSPVLAGEMTVQDIILNDHSWYADNNIKLHTGKTVSRIDRLKRKVIAEDGTEESY
                     DRLLLATGSTPFMLPVPGNQLPGVISYRDIKDTDEMIEAAKLYQHAVVIGGGLLGLEA
                     ANGLKLRGMDVTVVHAAGWLLERQLDETAGKMLQKSLEDRGLKFLLDKMTEALIAGES
                     GRVAAVRFKDGMQIPADLVVMAVGIRPNTALAESAGIYCNRGIVVNDTLQTYDPKIYA
                     VGECVAHRGVAYGLVAPLFEQGKVAANHLANFGIGRYTGSVTSTKLKVTGIDLFSAGD
                     FTGSSDTEEIVLNDAMGGVYKKLVIKDNKLVGGVLYGDTADGPWYFQMLRDGQDIHEI
                     RDHLMFGQSHVGDVGHQGHSKAAAMADSAEVCGCNGITKGTIVKAIKEKGLFTLDDIK
                     KHTKASSSCGSCANLCEQILSATIGGAYTPAASNRKPVCGCTDHSHQEVRDTIRQQHL
                     IKIADIMHFMEWKTPDGCDKCRPALNYYLISTWPKEARDDPRSRYINERAHANIQKDG
                     TFSVVPRMWGGLTTSAELRRIADVAEKYQVPTIKVTGGQRIDLLGIKKEDLISVWKDL
                     DMPSGHAYGKSIRTVKTCVGMEHCRFGTQLAMNMGVKLEKMLFDMYAPHKVKLAVSGC
                     PRNCAEAGIKDVGVIGVDSGYELYVGGNGGIKTEVAQFFVKVKNDEEVMEYAGAFLQL
                     YREEGFYLERTCHYLERVGLDHTKSKVVEDDDNRKALYARLLAELKDAKDPWAERVAG
                     VAKQEYEMLSV"
     gene            5533..5841
                     /gene="nirD"
                     /locus_tag="PLD53_00825"
     CDS             5533..5841
                     /gene="nirD"
                     /locus_tag="PLD53_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HRC59475.1"
                     /translation="MADWKMICNIEDIPKLGSRVVKSAEGEIAVFRTSSDEVFALRDK
                     CPHKGGPLSQGIVHGTQVTCPLHSWKLHLDSGEVVAPDVGCVRRYPTRVDGNAVFLEI
                     "
     gene            5863..7080
                     /locus_tag="PLD53_00830"
     CDS             5863..7080
                     /locus_tag="PLD53_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HRC59476.1"
                     /translation="MRADKSFWQAGHRPTLLAAFLYFDLAFMVWVMLGPLGIEIARDL
                     ALTHAQKGLMVATPVLSGAILRIVMGLLVDHLKPKMAGAIGQVIVIASLFVAWQFGVH
                     SYQQALVLGVFLGFAGASFAVALPLASRWYPPEHQGTALGIAGAGNSGTAIAALIAPS
                     LAIAYGWTNVFGLALIPLVLVFVFYVIVAKDAPECPPPKSFAEYMNVLKDKDSWWFMF
                     FYSVTFGGFVGLASSLTIYFNTQYGLDARMAGFFTAACVFAGSLVRPIGGNVADRIGG
                     IKSLSVMYVVAAIFLAIVSVGLPQAWMALAAFVCAMLALGMGNGAVFQLVPQRFRKEI
                     GVMTGLVGMAGGVGGFYLASSLGFAKQATGSYQIGFLIFAALAVLALAGLTGVKTRWR
                     TTWGAAHLTTARI"
     gene            7113..>7928
                     /locus_tag="PLD53_00835"
     CDS             7113..>7928
                     /locus_tag="PLD53_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HRC59477.1"
                     /translation="MPLSVSLGHSSLTGPRKRNEDFCGVVTPEGVELENKGLIAAVAD
                     GVGGHKGGREAAEYTVRGLLSDYYATPDTWSVPHALEKVIVPLNRWVMAEAGRQPELA
                     GMATTLSVLVLRGRRYVCAHVGDSRAYLLRDGAMAQLTVDHVWEHPELKNVLSRAVGL
                     DRQFHLDFVDGELIEGDCFLLCTDGVWGVLGDERIRDTLLAHPDAQQAAASLTSLAVA
                     ADGQDNASAVVIRIERLPQQNLRDSLEGSARLPLPPRFKPGQEFDGLRIEEEVH"
     assembly_gap    7929..7938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7939..8815
                     /locus_tag="PLD53_00840"
     CDS             <7939..8815
                     /locus_tag="PLD53_00840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRC59478.1"
                     /translation="VLHDSRATLLYRVTDQDSGQVRVMKTLRPERADDADEIRALIME
                     EWRARRVLSPFFAQIVPAEGRSCLYYLQTWHEGATLQRMLDTDLHFTVADCVRHGVRL
                     LKGLSSLHRLDIVHRDIKPANIHIGRDGNLRILDLGVAVSLSEDEANAGNPGTPSFMA
                     PELFSGEEAVAAHDLYAAGVTLYHLLTRKYPYGEVEPFQHPKFGEPVVPTRFRPDIPG
                     WLENVLLKSVSKDSRQRFETAEEFLLALERGANRPVSRPRVTPLAMRNPVLTWRIIAG
                     VSLALNLVLLMMLMR"
     gene            8826..9221
                     /locus_tag="PLD53_00845"
     CDS             8826..9221
                     /locus_tag="PLD53_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRC59479.1"
                     /translation="MPKTALILFAHGARDPEWASPMRRVCAAVHAQAPELRVELAFLE
                     FMTPTLRDCAQILLAENYRRLIVLPMFIAQGGHLKHDLPQLIDELREQNPQVSFELAG
                     AVGEVKSVVQAMADHVITLADGKADWALR"
     gene            complement(9214..9969)
                     /locus_tag="PLD53_00850"
     CDS             complement(9214..9969)
                     /locus_tag="PLD53_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRC59480.1"
                     /translation="MAQNSIEVSEEGGVRYLHFGSDWVQGAMRIQRPNALELPYTRDM
                     MAGLLLRAAPWPKNALLIGLGSGSLAKYIYHRLPDTQITVIEIDPQIKIIAHQHFKLP
                     DDPARLRVIIGDGATYVLEDVEEFDYILVDGFDKSGSAGALDTLSFYQACRAHLTAEG
                     LLAVNLLGRSRGFQASAERIAKAFDGRSLVFPSCSSGNTIAFATAGNTVDVTLDDLIK
                     RANHVDKTTGLNLKPTIPRLQIDNKLMQDRLVI"
     gene            complement(10087..10305)
                     /locus_tag="PLD53_00855"
     CDS             complement(10087..10305)
                     /locus_tag="PLD53_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRC59481.1"
                     /translation="MMLTEEESRHTRATLNELQVEHRDLNLVIEHLVSTPLPEQDQLL
                     IHRLKKRKLLLKDKIYHLESLLVPDIEA"
     gene            10635..10955
                     /locus_tag="PLD53_00860"
     CDS             10635..10955
                     /locus_tag="PLD53_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59482.1"
                     /translation="MLYHVTVSFGKDRQYQYRFSQAELAEGTPEEARRWFDKEFADLQ
                     CEASNPMGKVLIIDKILNIARYASERRFIDAKEWATLFARYTALALGRDTIRVDVEAF
                     NIGY"
     gene            11030..11590
                     /gene="greB"
                     /locus_tag="PLD53_00865"
     CDS             11030..11590
                     /gene="greB"
                     /locus_tag="PLD53_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HRC59483.1"
                     /translation="MSKAFVSETDGDDEDEFGSVPELPQGTRNYITPGGYARIKEELD
                     HLLRTERPQLVEVIHWAASNGDRSENGDYIYGKRRLREIDRRIRFLTKRLDLSEVVDP
                     ARRENADQVFFGATVTLSEELGPEQVYQIVGVDETDSQRGRISWVSPLARALLGAQEG
                     ARVRFRCPSGWREVEVLEIDYRSIEP"
     gene            11587..13773
                     /locus_tag="PLD53_00870"
     CDS             11587..13773
                     /locus_tag="PLD53_00870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRC59484.1"
                     /translation="MSVDFPVERIMKRDLLECAPSMSVREASKRMCEAGCGSIVVVDE
                     GRPVGIWTESDALSGAWHSTADLDQPVSTFMSTPVQSIPAQTTLGEATRHFRLAGVRH
                     FLVNDDQGHHKGIISQTDVVRSQGVAFFMRARIVGSLIQEPPNCVEMDTSFGEVRQLM
                     LERNLDAVIVRSGEHYGIITKRDVVGALSQQKIEANAGELASFPLVTIRHDATLLQAR
                     DVFIQNHIRHLGLMDDQQMPIGLLTFRDLFDTVEHEYVNGLLPELELQTERLLQSQRE
                     IARQVSLTDAILNALPINVFVKDEKGRLIIANEMSAKTTGRPLAEIIGRTDDELFPPE
                     VAKRLLADDARVRSANQTLVREELLDDGRTLLARKCLVQVDGAELLIGASMDVTDWKR
                     ADALMVSSHHVLELIAGGSELTVVLETLCKRMETHLPGSSCSILLLDADGQHLRHAAA
                     PSLPETYALAVDRVSIGPSAGSCGAAAFLGEQVIVEDIANSPLWADRLDFAKQYNWRA
                     CWSTPFFSAARKVLGTFAISYPHTKRPDYNDLMVITHATRMASVAVERWQQITELQRL
                     ATTDQLTDLSNRAHFLDNAEVELRRAGRFNRELVVLMIDIDLFKQINDRHGHATGDEA
                     LRVFSRVLGKETRAFDLLGRIGGEEFAVVLPETSIEAGLQIAERLREAVEKSSFVFHD
                     GPSISFTVSIGASRLQAGDNLDSLLARADDALYRAKHAGRNRIERA"
     gene            13954..15873
                     /gene="htpG"
                     /locus_tag="PLD53_00875"
     CDS             13954..15873
                     /gene="htpG"
                     /locus_tag="PLD53_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRC59485.1"
                     /translation="MGANKETLGFQAEVKQLLNLMIHSLYSNKEIFLRELISNASDAC
                     DRLRFEALNNAALYGGESGSDTELSIRLSFDKAARTLTISDNGIGLSRDEAVEHLGTI
                     AKSGTREFFSALTGDQAKDAHLIGQFGVGFYSSYIVADKVTVISRRAGLESNQAVRWE
                     SAGEGEFTVEMVEKAGRGTEVTLHLREGEDEFLSGWKLRQTVRKYSDHITLPILMKKE
                     KWDEEKKEQVETDEDETINQASALWARPKSEISDEQYKEFYKHVAHDFEEPLAYVHAK
                     VEGKQEYTQLLYIPSKAPFDMWDRNARHGIKLYVRRVFIMDDAEQLMPLYMRFVRGIV
                     DSNNLPLNVSREILQESRDIETIRGGCVKKVLSMLESMADSEDAAEKEKFARFWKEFG
                     RVLKEGTGEDFANKERIAKLLRFASTHTDTPDETVSLADYVSRMKEGQEKIYYVTAET
                     FTAAKNSPHLEIFRKKGIEVLLLSERVDEWVTGNLTEFEGKALQSVAKGGLDLGKLED
                     EAEKKEAEQAADEYKELLDKIKTTLGEKVKEVRVTHRLTDSPSCIVADEHDLGGNLAR
                     ILKAAGQKAPDSKPILEINPKHPAVQRLKYEESRFDDWANLLLEQATLAEGGTLDDPA
                     GFVKRINDLMLALSK"
     gene            15986..16468
                     /locus_tag="PLD53_00880"
     CDS             15986..16468
                     /locus_tag="PLD53_00880"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HRC59486.1"
                     /translation="MIADSLEGFPPILDSGVRTLVFGSFPSPASLAAQQYYAHRQNQF
                     WRIMAAITGEPLVAYGYAEKQRCLLRHRIGVWDVYRCCQRQGALDSAIQAAERNEFSR
                     LAIEAPNLQRVCFNGKTSGKFERYFRELGYATCVLPSTSPAYTLPFERKLEIWRSGVC
                     "
     gene            complement(16562..17104)
                     /locus_tag="PLD53_00885"
     CDS             complement(16562..17104)
                     /locus_tag="PLD53_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59487.1"
                     /translation="MKTMFIATMLTAALITVPAIAQQGPAGVPGAPGLAETAKSAPPS
                     SRPRHRQRGPADCRTAKDVELCTARQEAHKTALEACKGKTGVERQQCMHAQAQNLDCT
                     KAGNPQRCESRKHAYATCSAQSGPKFKQCVQQTMSTETCRKSADAARCELHQQARERC
                     KNLSGQEHRQCLRDVLAPKK"
     assembly_gap    17222..17321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17448..19241
                     /locus_tag="PLD53_00890"
     CDS             17448..19241
                     /locus_tag="PLD53_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRC59488.1"
                     /translation="MSDPMANTVPEHRLTVAEILGLMVADELVPKADADALIAECRLK
                     RLVAHPLTIISEQKWKSLQAPHRPLTLDYIAEWFAGKLGLEYFHIDPLKIDFTAVTDV
                     MSSAYATRFGILPVQVTTLEVVFATTEPFLRDWENEIKPIIKKNIRRVISNPVDVARY
                     LIEFYNLARSVKKASQQSGQSAGLSSFEQLVELGRSNRQFDANDQHIVNIVDWLWQYA
                     FDQRASDIHIEPRREIGIVRFRIDGVLHQVYQIPMSVMAAMVSRIKILGRMDLVEKRR
                     PQDGRIKTRTADGQEAEMRLSTLPTAFGEKLVMRIFDPEVLVRNFSDLGFSEDDQARW
                     KAMSQSPNGVILVTGPTGSGKTTTLYSTLKQLATPAVNVCTIEDPIEMVEASFNQLQV
                     QNSIDLDFAQGVRSLMRQDPDIIMVGEIRDLETAEVTIQAALTGHLVLSTLHTNDSPA
                     AVTRMLDLGVPSYLISATVLGIMAQRLVRLLCPLCKKSNPATAEDEAMWDRLVAPWKA
                     NRPSHFHQPGGCLECRMTGYRGRVGIYEILMLSADMKQAITNNADVAKIRDLAYREGM
                     KPLRISGAMKVAAGITTLAEVFKVAPPSERT"
     gene            complement(19312..19839)
                     /locus_tag="PLD53_00895"
     CDS             complement(19312..19839)
                     /locus_tag="PLD53_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59489.1"
                     /translation="MSITTTLTACVRFVKASPSRLGPFSLIDLSLFLALIIAAALGYK
                     YSFLLLPRADLNLAPVAGCDLHTQACPVDMPGGGRIELSITPHPIRVLKPLQVNATMT
                     GIAVNKVEIDFSGVDMDMGYNRQVLISDGNGHYAGETMLPVCITGRMAWRLSLIIETD
                     RQRIAVPFVFETPGR"
     gene            complement(19847..>20823)
                     /locus_tag="PLD53_00900"
     CDS             complement(19847..>20823)
                     /locus_tag="PLD53_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC59490.1"
                     /translation="AARHEAERLAAEQAARQEAERLAAEEAARQEAELLAAEQAARQE
                     AERRAAEQAEQLAAEEAARQEYERLVAEKAERREAKRLAAEEAARQEAERLVAEEAAR
                     QEAERLAAEEAAHQEAERLAAEEAARHEAERLAAEQAARQEAERLAAEKAYRQEAERL
                     AAEEAARQETERLAAEEARSRALAAASGTVAKISKTPEQTVVMVADDSKVVRIKTGRL
                     LTAHRFQVQMAEDGLDAASQIEESVPDVLLTDVDMPGLNGMQLVRKLRGNSRTAQLPI
                     IMVTSDSEELLDEAMSAGVNVVLGKPYSEEQLIAHIEQLMIMNGQSGV"
BASE COUNT         4241 a         5569 c         6109 g         4694 t
ORIGIN      
        1 agtgagaggg cacgatcatg caagttgagc atcacgagct tcaccgcgag ttccccgagt
       61 atctggaagt aatgcaagca ttgagaaaga ctgatagtag tttcagcaaa atattcgacg
      121 agtacgatag cctgacaagt gaagtggagc gcctggagga agaggacatc ccggttgacg
      181 atttcacgat cgaagaaatg aagaaagtac gcgtgttgct caaggacaag gtttatcaga
      241 tcctggttgc acacaaggac agcaactgaa cttaggccct ggccgatgcg tccggtaaaa
      301 tcgccggatg catcgcatcc cctactggcg tctttccggc ttttacttct tctacttcgc
      361 cttcatcggc gtttttctgc cctacttcgg gctgtatctt caaacccttt cgttttcggc
      421 ctgggacatc agcgttctga tgtcgcagat gcagttgatg cgactcttcg gtccgtatct
      481 ctggggcact ctggcggacc gcttcgtgtc gcgggtaccg atcattcgcc tggctggctt
      541 gatcgcgctg atggcgtctg cgtccttctt ccatatccgc agtttcgaag cgattctggt
      601 ggcaatggct gtcctgtcct ttttctggtc ggcaacgctt ccgctggttg aaacgctgac
      661 ttttgaacat ctgcgggaaa atccggaacg ctatagccgg atcagaatat ggggatccat
      721 tggtttcatt gttgccgtca tgggcgctgg tgccttgctg gatcatgtga agcttccaag
      781 catgctctgg atgatcgtgt ttgcgttggc cgggacgctt ctgtacgcca tcgtgatacc
      841 cgaggcagag ccagctctgc atcatgccga tcatcctccg gttttggaaa tactccgtca
      901 gagacgcgtg gtggcgctgt tctccgcctg cttttcaatg tctgctgcgc atggggcgct
      961 gtacgtcttc tactcgatct tcctgtccga tcatggctac agcaagtcgg tagtcggggg
     1021 tctatggtcg ctaggtgtgc tcgccgaaat tgcggttttt ttctatatgt cgcgtttgct
     1081 gcgggttttc ggcctgcgca ccattctgct gttgagtttt tccgcggccg tggtgcgttt
     1141 catgatgatc ggctggggtg tggagcagcc gttcgtgctt gtctttgcac aagtactcca
     1201 cggcctgact ttcggctcgt tccatgcggc ggcaatcacg gtggtcaatc gctggtttct
     1261 tgggcgcacc cgttcgcgcg gccaggcact gtattcaagc ctgaccttcg gagcaggtgg
     1321 cctggtcggt ggtttgatca gcggatggac atgggatcat ctgggtggcg aactgacctt
     1381 tgccttgagt tcgatttttg cgctgatcgg attgatactt gtaatggtct ggattcgtga
     1441 aacggacgtg gcaacgcatc aggtgcccta tccggtttga gggtacagaa ttctgcttgg
     1501 tagccgcgcc ttgccttgca actcccataa gcgcacactc catcatggcc gactctattc
     1561 gaagtaggcc agcagttaaa aaaacaatag tgccgccgga gagggatgca atgcttaaga
     1621 agtttcgttc gcgtttgcga aaactccttc atgtgtcgat aagaaaactg atttcctggt
     1681 tgccgcgcaa tcagcgcttt gctgttttcc gttcttttgc agattgcaat cctgctccca
     1741 gcaaacgact ggtattgaaa atcgccgaaa ccaaagagga gcttgaagcc tgcttctcgg
     1801 ttttgcacga tgcctacgtc gatagtggct tcatgaagcc cgacccgtcg ggcatgcgcg
     1861 tgacgattta ccatgcattg ccgaccacaa ccacgctttg tgccaagtac gacggcgagg
     1921 tggtcgggac gatctcgctg gttcgcgaaa gcgcattggg cgttccgtta caaaccatct
     1981 tcgatctcac acccttgcgg caaaaggaag gattcatcgc cgaggtgtcg gcacttgctg
     2041 ttcaccgcaa gtttcgcaag acaggtggca gcatcctttt cccgctgatg aaattcatgt
     2101 acgagtactg cacgactttt ttcgataccc ggcacttggt catagcggtc aatccaagcc
     2161 atatcgagat gtacgagtcc ttgctgttct tcaagcggct gacggcaacc atggtcgaaa
     2221 agtacgactt cgtgaatggc gccccggcga ttggcgccac cgtggactta aaggaagtac
     2281 tcgacacttt tcgcgagcac tatgcttcga agccgccgca ccgcaatctc tatgcttact
     2341 tcacgcaagt caaaatgccc aacatccagt ttcccagtcg acgtttcttt acgaccaacg
     2401 atccggtgct gacaccagag cttcttgatc acttcttcaa tgtgcagacc aaaaccttcg
     2461 aaaacctgaa cgaaaggcag aaggccttac tccattccat ttacgatctg ccggcgtact
     2521 ggaagattct gcctgctctt cccgacagtg ctgaagttat tccgatacgg cgtcaccagc
     2581 gtttctcggt gagatgtccg gcacgtatcg tcgttgccac atccgatgag cggcaggaaa
     2641 tcgtgatgga ggtggttgaa gtttctcgct acggcctgca ggcacgcgca cgcgatccac
     2701 tgccccttaa tacctggtgt gacctgaccg ttcaggtagg tcgctccgat gtcgcgcatt
     2761 tccgtgcttt ggcattgcgc gaaaacgaga atggcttcaa tggcttttat ggttttcgtt
     2821 tgggcgaacc tgacgtcgtc tggcaaaaat tcgtttccgc actnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnngtaaata cacttataaa tcaataaaca gaatgtctgg
     3001 cacgaggatt gcggtaagtg cttcatcaag aggcatttac tggagtcgag aacatgagca
     3061 agcccaaact ggtgatggtt ggcaatggca tggcaggcgt gcgtacgctt gaagagctgt
     3121 tgaagatttc acccgaccac tacgacatca ccgtcttcgg cgccgagccg catcccaatt
     3181 acaaccgcat actgctttca ccggtgctgg ccggcgagat gacggtacag gacatcattc
     3241 tgaacgatca ctcatggtac gccgataaca atatcaagct gcataccggt aaaaccgttt
     3301 cacgaatcga tcgtttgaag cgcaaggtca ttgccgagga cggcacggaa gagtcttacg
     3361 accgattgtt gttggctacc ggatcaacac ctttcatgtt gcctgttccg ggcaatcagt
     3421 tgccgggtgt catttcgtat cgcgacatca aggatacgga tgaaatgatc gaagcggcga
     3481 aactgtacca acacgcagtg gtcatcggcg gcggcctgct cggtctggag gcggccaacg
     3541 gccttaagtt gcgcggcatg gatgtcactg tggtgcacgc cgccgggtgg ttgcttgagc
     3601 gccagcttga cgaaacggct ggaaagatgt tgcagaagtc gcttgaagac cgtgggctga
     3661 aattccttct ggacaagatg acggaggctc tgatcgccgg cgaaagcggg cgcgtggctg
     3721 cggttcggtt caaggatggc atgcagattc cggcagacct ggtggttatg gctgtcggca
     3781 tacgcccgaa tacggctttg gccgagtcgg ctggcatcta ctgcaatcgc ggcattgtgg
     3841 tcaatgacac tttgcaaacc tatgacccga agatatacgc cgtgggtgag tgtgtggcgc
     3901 accggggtgt ggcttacggt ctggtggcgc cgcttttcga gcagggtaag gtggcggcga
     3961 accatctggc caacttcggc attggccgct ataccggctc ggtcacttcg accaagctga
     4021 aagttacggg cattgacctg ttctccgccg gcgactttac gggaagttcg gataccgagg
     4081 aaattgtcct caacgacgct atgggcggtg tctacaagaa acttgtaatc aaggacaaca
     4141 aactggtcgg tggcgtgctc tacggtgata cggcggatgg tccgtggtat tttcagatgt
     4201 tgcgcgacgg ccaggatatt cacgaaatcc gcgatcatct gatgttcggc cagtcccatg
     4261 tcggcgatgt cggccatcaa ggacacagca aggcagccgc aatggccgac agcgcagagg
     4321 tttgcggctg caacggaatc accaaaggca ctatcgtcaa ggcgatcaaa gagaaagggc
     4381 tgtttacgct cgacgatatc aagaagcaca ccaaagcgtc gtcgtcttgc ggttcctgtg
     4441 ccaacctttg cgagcaaatt ctttcggcga ccatcggcgg tgcctacacg ccagctgcgt
     4501 cgaacaggaa gccagtgtgt ggttgtaccg accactccca tcaggaagtg cgcgacacga
     4561 tccgtcagca gcacctgatc aagatcgccg acatcatgca cttcatggag tggaagacgc
     4621 ccgacggttg cgacaaatgc cggccggcgc tcaattacta cctgatctcg acttggccga
     4681 aggaggcaag ggatgatccg cgttcgcgtt acatcaatga acgcgcgcat gccaacattc
     4741 aaaaggacgg taccttctcg gtggtgccgc gcatgtgggg cgggctgacc acttcggccg
     4801 agctgcggcg tattgccgat gtcgccgaaa aatatcaggt gccaacgatc aaggtgaccg
     4861 gaggccaacg gatcgatctg cttggcatca agaaggagga tctgatcagt gtatggaagg
     4921 atctcgacat gccttcaggg catgcctatg gcaagtcgat ccgtacagtg aaaacctgcg
     4981 tcggtatgga gcattgccgc ttcggtacgc aactggcgat gaacatgggc gtcaagctgg
     5041 aaaagatgct gttcgacatg tatgcgccgc acaaggtcaa gctcgctgtc tctggctgtc
     5101 cgcgcaattg cgccgaggcc ggcatcaagg atgtcggcgt gatcggcgtg gattcaggct
     5161 acgagttgta tgtcggtggc aacgggggca tcaagaccga ggtcgcacag ttcttcgtca
     5221 aggtgaagaa cgacgaggaa gtgatggagt atgccggcgc cttcctgcaa ctctaccggg
     5281 aagaaggttt ttacctggag cggacctgtc actacctcga gcgagtgggt cttgatcaca
     5341 ccaagagcaa ggttgtcgaa gacgacgata accgcaaggc gctctatgcc cgcctgctgg
     5401 ccgagcttaa ggacgccaag gatccatggg cggaacgcgt tgccggggtg gcaaaacagg
     5461 aatatgaaat gctgagcgtt tgaaggcgag acagaaatgc cgagcgtatt gttgaagaca
     5521 acggagcaat gaatggctga ctggaaaatg atttgcaata ttgaggacat cccgaaactc
     5581 gggtcgcgtg tcgtgaaatc ggcagagggc gagatcgccg tatttcgcac gtcgagcgac
     5641 gaagttttcg cactgcgcga caagtgcccg cataaaggtg gaccgctttc tcaagggata
     5701 gtgcatggca ctcaggttac ctgcccgttg cacagctgga agctgcatct cgattccggg
     5761 gaagtggttg cgccggatgt cggctgcgtg cgtcgatacc caacccgcgt cgacggcaat
     5821 gccgtctttc ttgaaatttg aagcaaccga tggaaactga gaatgcgtgc tgataaatcc
     5881 ttctggcagg ccgggcatcg cccgacgcta cttgccgctt ttctttactt cgatctggcc
     5941 ttcatggtct gggtcatgct cggccctctc ggcatcgaga tcgccaggga tctcgcgctg
     6001 acgcatgcgc aaaagggcct gatggtggct acaccggtgt tgtctggcgc catcctgcga
     6061 atcgtcatgg gacttcttgt cgatcatctc aagccgaaga tggcgggcgc cattggccag
     6121 gtgatcgtga tcgcctcgct gtttgtcgcg tggcaattcg gcgttcacag ctatcaacag
     6181 gcgcttgtgc tgggtgtgtt cctcggcttc gccggcgcct catttgcagt ggccctgccg
     6241 ctcgcttcac gctggtatcc tccggaacat caaggcacgg cactgggcat tgccggcgct
     6301 ggcaattctg gaacggcaat tgccgcactg attgcgccaa gccttgccat cgcgtatggc
     6361 tggaccaatg tctttggtct ggctttgatc ccgctggttc tggtttttgt cttttacgtg
     6421 atagtcgcca aggatgctcc cgagtgtccg ccaccgaaat cttttgccga atacatgaac
     6481 gtgctgaaag acaaggattc ctggtggttc atgttcttct attcggttac cttcggcggc
     6541 ttcgtcggtc tggcctcgtc gctgaccatt tatttcaaca cccagtacgg cctggacgcc
     6601 aggatggccg gcttcttcac cgctgcctgt gtttttgccg gttcgctggt acgaccgatt
     6661 ggcggtaacg tcgctgaccg gatcggcggc attaagagtc tctccgtgat gtatgtggtg
     6721 gcggcaattt tcctggccat tgtcagcgtt ggcttgccac aggcctggat ggcgcttgct
     6781 gccttcgtct gcgccatgct ggccctcggt atgggcaatg gcgcggtgtt ccaacttgtg
     6841 ccgcagcgtt ttcgcaagga gattggcgtg atgaccggcc tggtggggat ggccgggggg
     6901 gtagggggct tctatctggc atcgtcactc ggcttcgcca agcaggctac tggcagctat
     6961 cagatcggct tcctgatttt tgctgcattg gcagttctgg ctctggctgg actgaccggg
     7021 gtaaagactc gctggagaac gacatggggc gctgcgcacc tgaccactgc tagaatatag
     7081 aactctttca atgcggggca catgaacagc taatgccgct atccgtcagc ctcggtcatt
     7141 catcgcttac cggcccacgc aaacggaatg aagacttctg cggtgtcgtc acgccggaag
     7201 gcgtcgagct ggagaataag ggactgatcg ccgcagtcgc cgacggtgtc ggtggtcaca
     7261 agggggggcg cgaagcagcc gaatacacgg tgcgcggcct gctctccgat tactacgcga
     7321 cgcctgatac ctggagcgtg ccgcacgcgc tggaaaaggt gatcgttccc ctcaaccgct
     7381 gggtgatggc cgaggccggt cgtcaacctg aactggccgg catggccacc accctgagcg
     7441 tgctggtgct tcgcgggcgg cgctacgtct gtgcgcacgt tggtgattcg cgggcctacc
     7501 tgttgcgcga cggcgcaatg gcgcaactca ccgtcgacca tgtctgggag cacccggaac
     7561 tgaagaatgt gttgtcgcgc gcggtgggtc ttgatcggca gttccatctt gattttgtcg
     7621 acggcgaact gattgagggc gattgcttcc tgctttgtac ggatggagtc tggggcgttc
     7681 tcggagacga gcgtatccgc gacacgctgc tcgcccatcc cgacgcacaa caggcggcgg
     7741 cgtcgctgac cagtttggct gtggcagccg atgggcagga caatgccagt gccgtggtca
     7801 ttcggatcga aagactgcca caacaaaacc tgcgcgattc actggaaggt tcggcacgat
     7861 tgccgcttcc gccacgtttc aaaccgggac aggaatttga cggcctgcgt atcgaggagg
     7921 aggtgcatnn nnnnnnnngg tcttgcacga ttcgcgcgcc accctgcttt atcgtgtgac
     7981 cgaccaagac tccggacaag tacgcgtgat gaagacattg cgcccggaaa gggcagacga
     8041 tgccgatgaa atccgcgcgc tcatcatgga agagtggcgc gcgcgccgcg tcctgtcgcc
     8101 gttcttcgct cagatcgtgc ctgcggaagg gcgcagttgc ctctattacc tgcaaacctg
     8161 gcacgaaggt gcgacgctgc agcgcatgct cgataccgac ctgcacttca ccgtggcgga
     8221 ctgcgtacgg cacggcgtcc gtctgctgaa agggctttcc agcctgcatc gcctggacat
     8281 cgttcaccgc gacatcaagc cggcgaacat ccatatcggc cgtgacggca acctgcgtat
     8341 cctcgacttg ggcgttgctg tcagcctgtc cgaggatgag gcaaatgcgg gcaatccggg
     8401 aacacccagc ttcatggcgc ccgagctgtt ttcaggcgag gaagccgtgg ccgctcacga
     8461 cctctatgca gccggcgtga cgctgtatca cttgctgacg cgcaagtatc cctacggtga
     8521 ggtcgaaccg ttccagcatc caaaattcgg tgaaccggtg gtgcctacgc gcttccgccc
     8581 ggacattccc ggctggctgg aaaacgtttt gctgaaatcg gtgtctaagg attcgaggca
     8641 gcgttttgaa accgccgagg agttccttct ggcgctcgaa cgtggcgcaa accgacccgt
     8701 gagccggcca cgagtcacgc ccttggccat gcgaaatccc gtgttgacct ggcgcatcat
     8761 cgccggtgtg tcccttgctc tcaatctggt attgctgatg atgttgatgc gataatgacc
     8821 gggtcatgcc aaaaactgca ctgatccttt ttgcccacgg cgcaagagac cccgagtggg
     8881 cgtcgccgat gcgccgtgtt tgcgctgcgg tgcacgcgca ggctcccgaa ctgcgagtcg
     8941 agcttgcttt cctggagttc atgacgccga ccttgcgtga ttgtgcccaa attctgcttg
     9001 ccgagaacta tcgccgcctc atcgtgctgc cgatgtttat tgcccagggg gggcacctca
     9061 aacacgattt gcctcagttg atcgatgaat tgcgcgaaca aaacccgcag gtcagctttg
     9121 aactggcggg tgccgttggc gaagtcaaaa gcgtcgtgca ggccatggct gatcacgtga
     9181 taacgctggc tgacggaaag gctgattggg cgctcagatg accagccgat cctgcatcag
     9241 tttgttatcg atttgaagtc ttgggatcgt tggcttcagg ttcaggccgg ttgtcttgtc
     9301 gacgtgattt gcgcgtttga tcaggtcatc gagtgtgaca tcaacggtat tgccggctgt
     9361 ggcaaaggcg atagtattgc cactgctgca cgaaggaaaa accagtgaac gaccatcgaa
     9421 cgctttggcg atacgctctg cgctggcctg gaatccccgg ctgcggccga gaagattgac
     9481 cgccagcagg ccttccgctg tcagatgtgc gcggcaagcc tggtagaagg aaagcgtgtc
     9541 aagcgcaccc gcgctgccac tcttgtcaaa tccgtcgacg aggatgtagt cgaattcctc
     9601 tacgtcttcc agcacgtatg tcgcgccgtc accgatgatg acacgcagtc gcgccgggtc
     9661 gtcgggcagt ttgaagtgct ggtgggcaat aatcttgatc tgtgggtcga tttcgatgac
     9721 cgtgatttga gtatcgggca acctatggta aatgtacttg gccagcgaac cggagccaag
     9781 gccgatcagg agcgcgtttt tcggccacgg cgcggcgcgt agcagcaggc cggccatcat
     9841 gtcgcgcgta tagggtagtt cgagggcatt ggggcgctgg atgcgcatcg cgccttgtac
     9901 ccagtcactg ccgaaatgca ggtagcgaac gccgccttct tcgctgactt cgattgaatt
     9961 ttgagccatg ggtgactcag gggccgttgg cagaatagta acgcttcgtc gccgttggca
    10021 taagcacaag tcgtttcgtg ggctgtatcg ttgtcaggtc aagccggttt gagcgggttg
    10081 aaggtttcaa gcctcgatgt cgggcaccag caagctttca aggtggtaaa tcttgtcctt
    10141 gagcagtaat ttccgtttct tcagccgatg tatcaataat tggtcctgct cgggtagcgg
    10201 cgtgctgacc aggtgttcaa tgaccaggtt gaggtcgcgg tgttccactt gaagctcatt
    10261 caaggtggcg cgggtatggc gtgattcctc ttcagtcagc atcattcgaa tatccgagaa
    10321 ttgacaatcg ctgcatctaa atccaaccat ctttccgtcc gtaaccacgc tcccaccata
    10381 caatgcatac aacaggcatc cagtaggacg ggcgtggcgg gttcgggttg ggggtagtca
    10441 agtggtatgg taggcgtacc ctcgagaaaa aacaacggcg agggagggtg cgttccatat
    10501 gaatgcgatc aagatgcgaa ggttcgggat tcgttctaca ctattccatt gattcagggc
    10561 ttgcggcttt tgggggtttt gcgaatagaa tagacgggaa tcttgatatt cctggcccag
    10621 gatggagaag gcaaatgctt tatcacgtaa ctgtttcatt tggtaaggat aggcaatacc
    10681 aatacaggtt ttcccaggct gagctggctg aaggcacgcc ggaggaggcc cgtcgctggt
    10741 tcgacaagga gttcgccgac ctgcagtgcg aagccagcaa cccgatgggc aaggtattga
    10801 ttattgacaa gatactcaac attgcgcgtt atgccagcga acggcgcttt atcgacgcca
    10861 aggaatgggc tacgctgttt gcgcgttaca cggccctggc attggggcgc gatacgatac
    10921 gggttgatgt tgaggcgttc aacatcggct attgatcgat tgatgcgatt caatacggcg
    10981 ctggctattg ttctggtgcc gtttctaacg gacttgccgg cgatatttca tgagcaaggc
    11041 gtttgtcagt gaaactgacg gcgatgatga agacgaattt gggtccgtgc cggagcttcc
    11101 ccaaggaact cgcaactaca tcacgccggg tgggtatgcg cgaatcaagg aagaacttga
    11161 tcatctgttg cgtaccgaac gtccgcaact cgtagaggtt atacattggg ctgcgtcaaa
    11221 cggtgatcgt tcggagaatg gcgactacat ctacggcaag cggcgcttgc gcgagatcga
    11281 tcgacgtatc cgtttcttga ccaagcggct tgatctatca gaagtcgtcg atccggcccg
    11341 ccgtgagaat gctgatcagg tgttcttcgg cgccacggta accctcagtg aggagctcgg
    11401 acctgagcag gtttatcaaa ttgtcggtgt cgacgagacc gattcccagc gcggacgaat
    11461 cagctgggta tcgccgttgg cgcgtgcatt gctcggtgcg caggaaggcg caagagtgcg
    11521 tttcaggtgt ccttcgggtt ggcgggaggt agaggtgctg gaaattgact atcggagtat
    11581 cgaaccatga gcgttgattt tccggttgaa aggatcatga agcgggatct gctcgaatgt
    11641 gccccgtcaa tgtcggtacg cgaggcgtct aagcgcatgt gcgaggcagg ttgtggctcg
    11701 attgtcgtgg tagacgaagg caggccggtg ggtatctgga ccgaatcgga cgccctgtcg
    11761 ggcgcctggc attccaccgc agaccttgat caacccgtgt cgaccttcat gagcactccg
    11821 gtgcagagca tccccgcaca aacgactctg ggcgaagcca cccgccattt tcgcctggct
    11881 ggagttcggc attttctggt caatgatgac caaggtcacc ataagggaat catttcacaa
    11941 actgacgtgg ttcgtagcca gggcgtcgct ttttttatgc gcgcgcgcat tgtaggctcg
    12001 ctgattcagg aacctcccaa ttgcgtggaa atggacacct ccttcggtga ggttcgtcaa
    12061 ttgatgctgg agcgtaatct ggatgccgtg atcgtccgtt ccggcgagca ctacggaatt
    12121 attaccaagc gcgatgtggt cggtgcactg agtcagcaga agatagaggc gaacgcaggt
    12181 gaattggcca gtttcccgct ggtgacgatt cgccacgatg ccacgctgct tcaggcacgc
    12241 gatgtattca ttcagaatca tatccgtcat ctcggtctga tggatgatca gcaaatgccg
    12301 atcggcctgc tgacctttcg cgaccttttc gataccgttg agcacgaata cgtcaacggt
    12361 cttctccccg agcttgagct gcagaccgag agactcctgc agtctcaacg tgagattgcc
    12421 cgtcaggtga gtttgaccga tgccattctc aacgccctgc cgatcaacgt tttcgtcaag
    12481 gacgaaaagg gccgattgat catcgccaac gaaatgtccg caaagacaac aggacggccc
    12541 ctcgccgaaa ttatcggacg taccgatgac gagttgtttc cccctgaagt cgccaagcgt
    12601 ttgctcgccg acgacgcgag ggtccgctcg gccaatcaga ctctggttcg cgaagagttg
    12661 ctcgacgatg ggcgtacctt gctggcccgc aagtgcctgg ttcaggtcga tggcgctgaa
    12721 ttgctgattg gtgcgtcaat ggatgtgacc gactggaagc gggccgacgc cttgatggtc
    12781 agcagccacc atgtgctgga actgattgcc ggaggtagtg aactgaccgt tgtgcttgag
    12841 accctgtgca agcgcatgga aacgcatttg cccggctcgt cgtgctcaat cctgctgctc
    12901 gatgccgatg gccagcacct gcggcacgcc gccgcgccga gcttgccgga aacctatgcg
    12961 ctagccgtag atcgcgtttc catcgggcct tcggcgggtt cctgcggtgc cgctgctttc
    13021 cttggcgaac aggtgatcgt tgaggatatc gccaacagcc cgctatgggc tgatcggctg
    13081 gatttcgcca agcaatacaa ttggcgggcg tgctggtcga cgccattttt ctccgctgca
    13141 cgcaaggtgc tgggtacgtt cgctatttct tatccccata cgaaacggcc cgattataac
    13201 gatttgatgg tcatcaccca cgccacgcgg atggcttcag tcgccgttga acgctggcag
    13261 cagattactg aactgcagcg gctggcaacg accgatcaac tgaccgatct gagcaatcgc
    13321 gcgcatttct tggataacgc agaagtcgag ttgcggcgtg cgggtcgatt caatcgtgag
    13381 ctggtggtcc tgatgatcga tatcgatctg ttcaagcaga tcaatgatcg tcatggacac
    13441 gccaccggtg acgaggcttt gcgcgttttt tcccgagtac tcggcaagga aacccgcgca
    13501 ttcgatttgc tgggccgaat tggcggtgaa gaattcgctg tggtgttgcc ggaaacctcg
    13561 atcgaagccg gattgcagat tgccgagcga ttgcgggagg cggtagagaa atccagcttt
    13621 gtattccacg atggtccgtc gatcagtttt acggtcagca tcggcgcctc gcggttgcaa
    13681 gccggtgaca atctggacag tctcctggcg cgcgccgacg atgcgcttta tcgggccaag
    13741 catgccggac gcaatcgtat cgaaagagct tgatcaccgc gcttgcgtcc tcgacatctc
    13801 ttttccacca gggcttgaaa ttggccggta tgccccaagc taggcaccca tcgccagata
    13861 cgtcaggttt tttctgattg aatggttgaa tccgcgattc agtcccgttc aatccgagat
    13921 ttcgatattt ctacaatttc caaggagttt tcaatgggcg ccaacaagga gaccctcggc
    13981 tttcaggccg aagtcaaaca gctgttgaat ttgatgatcc actcgctgta cagcaacaag
    14041 gaaatattcc tgcgagagct catctccaac gcctccgatg cctgcgacag actgcgtttc
    14101 gaggcgctga acaatgccgc gctgtatggt ggcgagtcgg gcagcgatac ggaactgagt
    14161 attcgcctgt cctttgacaa ggcggcacgt accttgacca tctccgacaa tggcattggc
    14221 ctttcgcgtg acgaagcggt cgagcatctg ggtactatcg ccaagtccgg cacccgggaa
    14281 ttcttctccg cactgaccgg cgatcaggcc aaggatgcgc atctgatcgg gcagtttggt
    14341 gtcggattct attcatcgta tatcgtcgcc gacaaggtta ccgtgatctc gcggcgcgcc
    14401 ggactggaat ccaatcaggc tgttcgatgg gaatcggcgg gcgagggcga gttcactgtc
    14461 gagatggtcg agaaggccgg tcgaggaacc gaagtgaccc tgcatctgcg ggaaggggag
    14521 gatgaattcc tctccggctg gaaactgcgc caaacggtac gcaagtactc cgaccacatt
    14581 accttgccca tcctgatgaa gaaagaaaag tgggacgaag agaaaaagga gcaggtcgaa
    14641 accgacgagg atgaaaccat caatcaggct tccgctttgt gggcgcggcc caagtcggaa
    14701 atcagcgatg agcagtacaa ggaattctac aagcacgtcg cccatgattt cgaagaaccg
    14761 ctggcctacg tgcatgccaa ggtcgaaggc aagcaggaat acacacagct gctctatatt
    14821 ccgtcgaaag cgcccttcga catgtgggac cgcaatgccc gtcacggcat caagctctac
    14881 gtgcgccgcg ttttcatcat ggacgacgcc gaacagctga tgccgcttta catgcgtttt
    14941 gtgcgcggca tcgtcgattc gaacaatctg ccgctcaacg tatcgcgcga gatactgcag
    15001 gaatcgcgtg acatcgagac gatccgtggc gggtgcgtca agaaagtcct gagcatgctg
    15061 gaaagcatgg ccgacagcga agatgcggcc gaaaaggaaa agttcgcgag attctggaaa
    15121 gaattcggtc gcgtgctcaa ggagggtacc ggcgaagact ttgccaacaa ggaacgcatt
    15181 gccaagctgc tccgctttgc ttcgacgcat accgatacgc cggacgagac agtctcgctg
    15241 gccgattacg tgtcgcgcat gaaagaaggc caggagaaga tctactacgt taccgccgag
    15301 acgtttaccg ccgccaagaa cagcccgcac cttgaaatct tccgcaagaa gggcatcgaa
    15361 gtcctgctgc tttccgagcg cgtagacgag tgggttaccg gcaatcttac tgaatttgaa
    15421 ggcaaggccc tgcaatccgt agccaagggt ggtctcgatc tcggcaagct ggaagacgag
    15481 gccgagaaga aggaagccga gcaggctgcc gatgagtaca aggagctgct cgacaagatc
    15541 aagacgacgc tgggcgagaa ggtcaaggag gtgcgcgtga cgcatcgtct gaccgattct
    15601 ccgtcgtgta tcgtcgcaga cgagcacgat ctgggcggca atctggcgcg cattctcaag
    15661 gctgcaggac agaaagcgcc ggacagcaaa ccgattctcg aaatcaatcc caagcacccg
    15721 gcggttcaac gcctgaagta cgaagaatcg cgtttcgacg attgggcaaa tctgctgctc
    15781 gaacaagcga ccctggctga aggcggtacg ctcgacgatc cggcgggctt cgtcaagcgc
    15841 atcaacgacc tgatgctggc gctgtccaaa tgatggatgg ctaatcgccg cggagttcgt
    15901 cgactgcgcc ttgccgtgcc atgagtacag cttcagcgtg tcttcgcgcc acgggcgatc
    15961 attggggtga tttgaagcgg agatcgtgat tgccgattcg cttgagggtt ttcctccgat
    16021 tctggacagt ggagtcagga cgctcgtttt cggcagtttt ccatcaccag cgtcactggc
    16081 ggcgcagcag tattacgcgc atcggcagaa ccagttctgg cgcatcatgg cagcgatcac
    16141 gggggagccg ctggttgcgt acgggtacgc cgaaaagcag cgttgtctgc tccggcatcg
    16201 catcggcgtg tgggacgtct atcggtgttg tcagcgccag ggcgcgctcg attcggcgat
    16261 acaggccgcc gaaagaaacg aattctcaag gctggcgatt gaagcgccga acttgcagcg
    16321 cgtctgtttc aatggcaaga cttccggcaa gttcgagcgt tactttcgcg aacttggtta
    16381 cgctacgtgc gtgctgccct cgaccagtcc ggcctatacc ctgccgttcg aacgcaagct
    16441 ggaaatctgg cgctcgggag tatgttgaaa gattgaccgg cacggatact cttacgatgg
    16501 tgccggtcac gccgttggcg aatcgaatga ttctcggtgc gaagcacgcc ggagcctttg
    16561 cttacttttt tggggccagg acgtcgcgta ggcattgacg atgctcctgg ccactgaggt
    16621 tcttgcagcg ttcccgcgct tgctgatgca gttcgcaacg cgccgcgtcg gctgattttc
    16681 tgcaagtctc ggttgacatc gtctgctgca cacactgttt aaacttcggg ccgctttgcg
    16741 cactgcacgt ggcataggcg tgcttgcgac tttcgcatcg ttgcgggttg cctgctttcg
    16801 tgcagtcaag gttctgggct tgcgcgtgca tgcattgttg gcgctccacc ccggtcttgc
    16861 ccttgcaggc ctccagagcc gttttgtggg cctcctgtcg cgccgtgcat aactcaacat
    16921 ccttggccgt acggcaatca gcggggcccc gctgtcgatg ccgcggccgt gaggaaggtg
    16981 gtgcagactt ggctgtttcg gccagccccg gtgcgccggg gacaccggct ggtccctgct
    17041 gcgcaattgc gggcacggtg atgagcgcgg cagtcaacat ggtggcaatg aacatggttt
    17101 tcatcgaagg ctcctggcga gatctggaaa atactgtttg ttgatcagaa cagcgtatca
    17161 agtgatgatt gtagtctctc caaaagcaaa cttccgaagc tgtcaggaga aaactggatt
    17221 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngattgtagt ctctccaaaa
    17341 gcaaacttcc gaagctgtca ggagaaaact ggattcaggt tgttgtcatt gagccgccca
    17401 cggcttgtca ggtatagttt gcgcatatct tccatggaag ctttctgatg tccgatccga
    17461 tggccaatac tgttcctgaa caccgcctga cggtagcgga aatccttggc ttgatggtgg
    17521 cggacgaact tgtcccgaaa gccgacgccg atgcgctgat tgcggagtgt cgcctgaagc
    17581 ggctggttgc gcatccactg accatcattt ccgaacagaa gtggaaatcg ttacaggcgc
    17641 ctcatcgccc gttgaccctc gactacattg cggaatggtt tgcgggcaag ctcggactgg
    17701 agtatttcca catcgatccc ctgaagatcg atttcacggc ggtaaccgat gtcatgtcga
    17761 gtgcttatgc gacccgcttc ggcattctcc cggtgcaggt cacaacgctt gaggtcgtgt
    17821 ttgcgacaac agagcccttt ttgcgtgact gggaaaatga gatcaaaccc atcatcaaga
    17881 agaacatacg ccgggtcatt tccaacccgg tcgatgtagc ccgctatctg atcgagttct
    17941 acaatctcgc gcgctcggtc aaaaaggctt cgcagcagag cggtcagagc gcgggcctgt
    18001 catcgttcga gcagttggtc gagctggggc ggagcaatcg ccagttcgat gccaacgacc
    18061 agcatatcgt caacatcgtc gactggctct ggcagtatgc tttcgatcag cgagccagcg
    18121 atattcacat cgagccgcgg cgtgagatcg gtatcgtgcg ttttcgtatc gatggcgtat
    18181 tgcatcaggt ctatcagatt cccatgagcg tgatggcggc gatggtcagt cgcatcaaga
    18241 tcctggggcg catggatctg gtcgagaagc gacggccgca ggacgggcgc atcaagacgc
    18301 gaacggcgga tgggcaagag gcggagatgc gcctttcgac gctaccaacg gcttttggcg
    18361 aaaaactggt gatgcgcatc tttgatccgg aagtgctggt gcgcaacttt tcagatctcg
    18421 gtttttccga ggatgatcag gcacgctgga aggcgatgtc gcaaagcccc aatggcgtta
    18481 ttctggtgac cggccccacc gggtccggaa aaacgacgac cctctactcg acgctcaagc
    18541 aattggcgac gcccgcggtc aacgtgtgca ccattgaaga cccgatcgaa atggtcgagg
    18601 cgtcattcaa ccagttgcag gttcagaact ccattgacct ggattttgca cagggcgtgc
    18661 gatcgctgat gcggcaggat ccggacatca tcatggtggg tgaaatacgc gatctggaaa
    18721 ccgctgaagt gactatccag gctgcgctca caggccacct cgtgctatcc accctgcaca
    18781 ccaacgactc tccggcggcc gtaacgcgca tgcttgatct gggcgttcca tcctacctga
    18841 tcagcgccac tgtgctcggc atcatggccc agcgtcttgt gcgattgctc tgcccgctgt
    18901 gcaagaaatc gaacccggcg actgccgagg atgaagccat gtgggatcgt ctcgttgccc
    18961 cctggaaggc caatcgcccc agtcatttcc atcagccggg aggctgcctt gaatgccgga
    19021 tgaccggcta tcggggacgt gtcggaatct acgagattct catgctgtca gcggacatga
    19081 agcaggcgat cacgaacaat gccgacgtgg ccaagattcg cgatctggcc taccgcgaag
    19141 ggatgaaacc cctgcgcatc tccggggcga tgaaggtggc cgcgggaatc accacgctgg
    19201 ccgaggtgtt caaggtcgcg ccaccgtctg aacgaacctg agtcacggcg ttgttaatgc
    19261 cggattgttt gttgcgctgg ctatcaggta tcggttgaat caaagcttcg ctcaacgccc
    19321 aggtgtttca aacacgaagg gtacggcaat ccgctgtcgg tcggtttcga tgatcagaga
    19381 taggcgccaa gccatgcggc cggtgatgca aacgggaagc atggtttcgc cggcatagtg
    19441 tccgttaccg tcggatatga ggacctggcg gttgtagccc atgtccatat cgacgccgga
    19501 aaagtcgatt tctactttgt ttaccgcgat gccggtcatc gttgcgttga cctgcagggg
    19561 tttcagaact cgaatcggat gcggggtgat actcagttcg attcggccgc cgccaggcat
    19621 atccaccggg cacgcttgtg tgtgcagatc gcaaccggca acaggcgcga ggttcaggtc
    19681 ggccctgggt agcagcagga acgagtactt gtagccaagt gcggccgcga tgatcagagc
    19741 caggaagagg gataagtcga tgagtgagaa tggaccaaga cggctggggc tggctttgac
    19801 aaatcggacg caggcagtga gtgttgtcgt gatgctcatc gcattcttaa acgcctgatt
    19861 gaccattcat gatcatcagc tgttcaatat gggcaatcag ttgctcttcg gagtatggtt
    19921 tgccgagcac cacgtttacc cctgccgaca tggcttcgtc aagcagctct tcactatccg
    19981 aggtcaccat gatgatcggg agctgggcgg tgcgcgaatt tccacgcagc ttgcgcacca
    20041 gttgcattcc attcaggccc ggcatatcga cgtcagtcaa caacacgtcc gggaccgatt
    20101 cttcgatctg acttgcagca tccagcccgt cttcagccat ctgaacctga aaacggtgcg
    20161 ccgtaagaag ccggcctgtc ttgatgcgta caacctttga atcatcggct accatgacca
    20221 cagtttgctc gggagttttc gagatttttg cgacagtccc agaggccgca gcaagcgctc
    20281 gacttcgtgc ttcctcggcg gccagccgtt cagtctcctg acgggcggct tcctcggcgg
    20341 cgagacgctc agcttcctga cgataggctt tctcagcagc caggcgttca gcttcctgac
    20401 gagcggcttg ctcggcggca agacgttcag cttcgtggcg ggcagcttct tcggccgcca
    20461 gacgctcagc ttcctgatgg gcggcttcct cggcggcgag acgctcagct tcctgacgag
    20521 cggcttcctc agcaactagg cgttcagctt cctgacgagc ggcttcctcg gcggccaatc
    20581 gtttggcttc cctgcgctcg gctttctcgg ctaccaggcg ttcgtactcc tggcgcgcag
    20641 cttcctcggc ggctaattgt tcggcttgct cggcggcccg acgctcagcc tcctgacggg
    20701 cggcttgctc ggcagcgaga agttcagctt cctggcgggc agcttcctcg gccgccagac
    20761 gttcagcctc ctgacgagcg gcttgctcgg cggcaagacg ttcagcttcg tggcgggcag
    20821 ctt
//
LOCUS       DAORPM010000009        20509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_447_length_20509_cov_10.366432, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000009 DAORPM010000000
VERSION     DAORPM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 20509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20509
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_20509_cov_10.366432"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLD53_00905"
     CDS             <1..255
                     /locus_tag="PLD53_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRC59491.1"
                     /translation="QQDSFVAGLLDCPHYTRPEEYEGQRVPDVLLSGHHEQIRRWRLK
                     QALGRTWQRRPELLAGRSLPKEETQLLVQFQEQCGQENKT"
     gene            278..646
                     /gene="rplS"
                     /locus_tag="PLD53_00910"
     CDS             278..646
                     /gene="rplS"
                     /locus_tag="PLD53_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRC59492.1"
                     /translation="MNLIQQLEQEEIARMGKTIPAFAPGDTVVVQVKVKEGTRERLQA
                     YEGVVIAKRNRGLNSNFIVRKISSGEGVERTFQTYSPLVAAIEVKRRGDVRRAKLYYL
                     RERSGKSARIKEKLTRKRKV"
     gene            complement(729..1544)
                     /gene="folE2"
                     /locus_tag="PLD53_00915"
     CDS             complement(729..1544)
                     /gene="folE2"
                     /locus_tag="PLD53_00915"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRC59493.1"
                     /translation="MNAPQAPIPNAMVDVQNLADTRQIAINRVGIKSIRHPVKVLDKS
                     GGVQHTIAMFNMYVGLPHNFKGTHMSRFVEILNSHEREISVENFPTMLREMLEKLEAE
                     TGHIEMNFPYFINKAAPVSGVQSLMDYDVTLTGEICHGEIASTIKVVVPVTSLCPCSK
                     KISERGAHNQRSHVTVTARINDHLWIEEIVQLVEAEASCELYGLLKRPDEKHVTERAY
                     DNPKFVEDMVRDVAARLSAEPRIYSYVVESENFESIHNHSAYALIENRNPEPV"
     gene            complement(1541..3451)
                     /gene="dxs"
                     /locus_tag="PLD53_00920"
     CDS             complement(1541..3451)
                     /gene="dxs"
                     /locus_tag="PLD53_00920"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRC59494.1"
                     /translation="MTSYPLLDTISNPAQLRALDRKQLPQLAEELRSFLVESVSRTGG
                     HLSSNLGTVELTVALHYVYDTPHDRLVWDVGHQTYAHKVLTGRRAGMSRLRTHGGVSG
                     FPKRSESDYDTFGVGHSSTSISAALGMALAVKLKGEARQTVAIIGDGAMSAGQAFEAM
                     NNAGVADADMLVILNDNDMSISPPVGALNKYLARLFSGSTFNSVRKTGEKVLRVSPSL
                     LEFANRVEEHVKGMLTPGTLFEELGFNYIGPIDGHDLDSLVPTLQNLQKLKGPQFLHV
                     ITRKGQGYKLAEADPILYHGVAKFHPEVGIENGKAGGKPSYTQIFGEWLCDMAAADPR
                     LIGITPAMGEGSGMIRFAERFPERYFDVGIAEQHAVTFAAGLACEGMRPVLAIYSTFL
                     QRGYDQLIHDVALQKLPVVFALDRGGLVGADGPTHHGTFDLSFLTCIPNMVVMTPADE
                     MECRNMLTTAFHIDGPSAVRYPRGSGPGVATDKALLDLPIGKGEIRRRGKGVALLAFG
                     SMLVPALGAAPEIDATVANMRFVKPIDRDLIIELAREHSLLISVEENALIGGAGAEVA
                     RVLDEIDSPAKLIRLGLPDHFIDHGDQALLLAEAGLDKEGIVRTVRVHQGSAPSSDQK
                     SGAISTAHQELQ"
     gene            complement(3514..4392)
                     /locus_tag="PLD53_00925"
     CDS             complement(3514..4392)
                     /locus_tag="PLD53_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRC59495.1"
                     /translation="MRTVQTRIEATLSRLLPPAEAIPARLHQAMRYASLNGGKRVRPL
                     FAFAAGELTGGSPEKLEIVSSAVELIHAYSLVHDDLPCMDNDVLRRGRPTCHVEYDEP
                     TALLVGDSLQSLAFELLARTDLGSESANSARQLEMVRLLAHASGSSGMAGGQAIDLGA
                     VGNVLDQPELELMHALKTGALIRAAVMLGALCGETLSDEARESLDRFAKRAGLLFQVV
                     DDILDCTVSTATLGKTAGKDAVDEKPTYVSLLGLERAREFADELGKQALDSLAIFGER
                     GQRLIELTAFITHRKF"
     gene            complement(4446..4733)
                     /locus_tag="PLD53_00930"
     CDS             complement(4446..4733)
                     /locus_tag="PLD53_00930"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRC59496.1"
                     /translation="MVRTNPANPHESGASQPATPAASEMKYEAAVAELEQIVQNMEAG
                     QLPLEESIAAYRRGCELLKYCQQQLSDAERKIQVLEDGSLREFDTAGGEAQ"
     gene            4732..4875
                     /locus_tag="PLD53_00935"
     CDS             4732..4875
                     /locus_tag="PLD53_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59497.1"
                     /translation="MGKGLIKLSKIAQEGASGQMETDRILVPQALLSAAYRLQASFAN
                     PSD"
     gene            4951..5301
                     /locus_tag="PLD53_00940"
     CDS             4951..5301
                     /locus_tag="PLD53_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HRC59498.1"
                     /translation="MIVIQHQPKRVEVNVYGEFTLADYKEFEDMVNYKVKFEGPVDLY
                     FDLREMADFTLDVAWEDIVFSRAHAKDFSRIAVLTLSQWVAWSAWLSQIFVRAEMRLF
                     DDEAEARAWLDESI"
     gene            5316..6155
                     /locus_tag="PLD53_00945"
     CDS             5316..6155
                     /locus_tag="PLD53_00945"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRC59499.1"
                     /translation="MNFLTLLSCDELSRHLNRPDWRVFDCRHSLSDVESGEKAYAEGH
                     VPGAFFLHLDRDLSGPMNGRNGRHPLPDPCCLASTLGATGVARDTQVVAYDDAGGMIA
                     GRLWWLLRWLGHDRVALLDGGINQWMKEGRELSTELTQSVPCVFDIARRDWVVSTESV
                     LANINKEGFCLIDARSPDRFRGENETLDPVGGHIPGARNRFFRDNLDDKGCFRPAAEL
                     RRDFLELLAGVEADKAVMQCGSGVTACHNLLAMEIAGLHGARLYAGSWSEWCADPARP
                     VER"
     gene            complement(6229..8547)
                     /locus_tag="PLD53_00950"
     CDS             complement(6229..8547)
                     /locus_tag="PLD53_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HRC59500.1"
                     /translation="MTQSSSSPAANRVISLNSLGRDRSPGRRILAECRDQLVNSLFVW
                     LREVAAPVSEELFLLADSTRERLQQTRYLDLRADVEKDWSHLVETFRRDLSAEADRCQ
                     NQEAEKDGKREASLEIPDFEGLQLVADDDLSEHIVIREFSAQLSETCDEELYALNRRV
                     AVLLGRDELTESDNPLAPPVVCRALSDACATIGSNAEERLLLLRRLERHLHLALPPIY
                     QQINAYLVERGILPDLKRNYRRSSGSAAKSATSEPDSSSPASMTPASPTTSGLNSSAG
                     ILDALQRLAQARVARLPAGLSSDGGNAMPGAMTSGPPGEVLDPESVSRLLFSSLNDLQ
                     HAPSCEPGGLIVNQVRLVRESESAQQVGGLEAVTIDIVAMLFDFIFDDAHIPVAVKAL
                     ISRLQIPVLKVAMLNPGFFADRQHPTRRFLGSVSGVSIRWGSLVDETDPFYCKLAELV
                     ERIQADFEDDVEVFGTALAELEAFVDECQVEEDSTALTAANIVILREQDAEGWERAQR
                     AVQAFRASNSPPALIDAFLTEHWVGVLHKIAVREDPNETDWQTAKEAMKDLSWSIEAK
                     KTPDDRLKLIGMLPKLLAQINKGLDSVDASPSQRSDFFDQLVQCHSAALKGETHATPT
                     PEIPPASGAENASPAFSPSEEGDLLVTRSTDNGVEVEEVILVGASPIWRADDREIFRL
                     VSELKRGDWVEFHELEGPDGDVASTNRERLNWISPQRGILLFSNHRSAKAISIAPEAL
                     ARQIRDGRATIVRDEPIFERALSGALESMNAR"
     regulatory      complement(8668..8758)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8894..10012)
                     /gene="queG"
                     /locus_tag="PLD53_00955"
     CDS             complement(8894..10012)
                     /gene="queG"
                     /locus_tag="PLD53_00955"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRC59501.1"
                     /translation="MQEFNSDTRTLTASAWNGFDFVALVKKIKNWGKELGFSELGIAG
                     ADVSTATPKLLRWLELGRHGEMNYMAKHAALRAQPETLVPGTLSVVTARLPYLPAAAE
                     CEQVLGNRELAYISRYALGRDYHKTIRNRLQKLANRIHAEILQSEQESRFAYRVFSDS
                     APVMEVEFARQSGIAWRGKHSLALTRSGSWHFLGEIYTTLPLPPDELISEHCGTCIRC
                     LEACPTAAIVAPYEVDARRCISYLTIELAGAIPVELRPLIGNRIYGCDDCQLCCPWNE
                     FAQLGDPDFAVRNGLDATSLATLFSWNEQAFMTRLEGSPIRRIGHTRWLRNIAVALGN
                     AANTAEASAALSTRLEHPSALVREHVRWALAQIHSDSS"
     gene            10067..10546
                     /gene="tsaE"
                     /locus_tag="PLD53_00960"
     CDS             10067..10546
                     /gene="tsaE"
                     /locus_tag="PLD53_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRC59502.1"
                     /translation="MYLPDEATTMVLGRELAPLLAPGMVVWLNGDLGSGKTALVRALL
                     RGLGHVGPVKSPTYTLVEVYVVSSLYWYHFDFYRISEPEEFEDAGLGEYFRDDAVCLV
                     EWPDKAAGYVPPADLSIMLRFAAGQPESGRDLEIVAHSEAGQRCLTALINRMPRVGS"
     gene            10501..11808
                     /locus_tag="PLD53_00965"
     CDS             10501..11808
                     /locus_tag="PLD53_00965"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRC59503.1"
                     /translation="MSNRTHQPHAARRELIKFAGASLVLLVSPVGRAAVNRKPSVLAV
                     RVWPADDYTRVAIECDTPLKFTHFSVKNPDRLVVDLEGIEFNSVLQALGNKVAADDPN
                     IKLLRAGRFKPGVVRLVMELKNEVNPQVFELPPAGEYGHRLVLDVYPLESPDPLLSLL
                     QKSEPSPASPGDRLEDKAVQNAENKASRSREGRPVVDRLITITLDPGHGGEDPGAVGR
                     GGSYEKHVTLAVAKRLKAKIDAEPNMRAVLTRNTDYFVPLHVRVQKARRIRSDLFVSI
                     HADAFIRPDAHGSSVFALSESGASSSAARYLAQKENASDLIGGVNLDVKDPMLARTLL
                     DLSQTATISDSLKLGKAVLGEIGGINTLHKASVEQAGFAVLKAPDIPSILIETAFISN
                     PQEERRLNDEAYQDKMAEAIISGIRKYFAKNPPLAKSKLASID"
     gene            complement(11931..12482)
                     /locus_tag="PLD53_00970"
     CDS             complement(11931..12482)
                     /locus_tag="PLD53_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRC59504.1"
                     /translation="MPISNYLDTSPQLAERIFLHESSQVIGDVRLGDDVSVWCNTVIR
                     GDVNHIVIGRCTNIQDFVMCHVSHKNENKPLGSPLIIGDYVTVGHSAVLHGCEIGNDC
                     LIGLGSIVMDDAKISSNVMLGAGSLVSPGMHLESGFLYVGRPAKRVRPLSTSELAYLR
                     YSAEHYLRVKNNYLAGQAANGGD"
     gene            complement(12615..13859)
                     /locus_tag="PLD53_00975"
     CDS             complement(12615..13859)
                     /locus_tag="PLD53_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRC59505.1"
                     /translation="MNNELLLGRQPVLDQHQKLVGYELMLQLEAGDKTPPTYNPSRAA
                     MLICAAYAELGIRSALGRNIAFLRFDPMFFHEDAIEAMPPDAVILELALETAPDAQTL
                     ARCRALRERDYSLALANYAGLDERSMPLLNMLDVIKIDIREHDENKLRELAGPLAKLP
                     LKLLAQGVETQEAMELSKKVGFELFQGYYFARPEIVSGRRLMASQISLIQLINLVSRD
                     LDTAKIEDGFKHEPALAANLLRIVNSVGYGLNRRISSLRHAITLLGRRQLQRWLQLLL
                     MTPAEETPDPSSSPLLQVAALRGRMMELLLRHSYPRNGTLADQAFITGIMSMMPTALG
                     LPMHEIFEQIALETSVMEALTSHAGVLGKTLALLECFDADDSQGCMHMLDQLPEAGLS
                     LGTLNSCLVESLRWVNGSTEEC"
     gene            complement(13895..15181)
                     /locus_tag="PLD53_00980"
     CDS             complement(13895..15181)
                     /locus_tag="PLD53_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC59506.1"
                     /translation="MSETQSPLIFLGRQPILGREQQLIAYELLFRDGVPAANGSADIT
                     DPTQATATVITNAFTELAAHAALGPYTGYINIDSDLLFSELIEVLPPQLVVLEILESI
                     SPTPEVVARCHQLQDKGFMLAVDDIAQIEEAYRPLLELTDIIKVDILKLDAERLPPLV
                     TQLKKFGKKLLAEKVETVKQHSQCQSLGFDLFQGYYFAQPTIIVGKKLNPTQVALLRL
                     LGMIMDDADTSEIENAFKVEPGLTVNMLRLTNSVSCGLSTKITSLRHAITILGRRQLQ
                     RWLQLLIYTNPQGNAQGINPLLQLAATRGRLIELLADRVQPRNREFSDHAFMVGIMSL
                     MPTLLGMTMVDILAQLPVAQRVKQALIEQPEQSGQLGQLLLIAAATEVTDPVELEEAL
                     HRVPAINLDFLGKCLARAFSWANNLGQEQDTTAVEQ"
     gene            complement(15259..16530)
                     /locus_tag="PLD53_00985"
     CDS             complement(15259..16530)
                     /locus_tag="PLD53_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC59507.1"
                     /translation="MPDNDLQEVFLGRQPILDRNQQLFAYELLFRSGSAESGNFASFV
                     DGNQATATVITNAFTEFSMADALGPYQGFIKVDHGLLFSDLIYALPTHSVVLEILETV
                     APTDEMLARCQLLRSEGFVLAIRERPESLDQSRPLLKLAEVIKVDISRVDPTRLKQLS
                     TNLKPLEKTILAEKVESNEQMLLCHGLGFDLFQGYYFAHPTVIKGKSLHTSELSLLRL
                     LGLLSQDADNAEIEIVFKQEPMLTFNLLRLTNSVGAGMATKITSLRHAITLLGRRQLL
                     RWLQLLLYSGISGERQAVNPLLQLAATRGRLMELLVERTPRAKAGGQELIDQAYMVGI
                     LSLMPTLVGHNMSDILKQLPVAQPVLDALGSRSGTLGDLLGLVESLEEDDGGANAAHL
                     LHGITGIDAKFANGCLTRALAWANNLARESS"
     gene            complement(16932..17327)
                     /locus_tag="PLD53_00990"
     CDS             complement(16932..17327)
                     /locus_tag="PLD53_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC59508.1"
                     /translation="MNEVLPPLGIQRVKVVAVSVTNLDRANRFYKEKMGLEPAFEGTE
                     QVGWWLGQVILMLKPDWAAPTDTPNPRVTLATEHAPATQDALRQRGITIANEVQVYGD
                     FYVGSFLDSEGNKLWFCSPATASEPPHGT"
     gene            complement(17718..18737)
                     /locus_tag="PLD53_00995"
     CDS             complement(17718..18737)
                     /locus_tag="PLD53_00995"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRC59509.1"
                     /translation="MSTTRTINAYAVLEAGGRFEPFSYEVGELHPDEVEIDVLHCGIC
                     HSDLSMVDNEWGMSKYPLVAGHEVIGRISARGSHVLNLEEGTVVGLGWHCGYCGHCHS
                     CKTGDQNLCAEAQSTIVAHHGGFADKVRADASSVIAIPEGIDLESAGPLFCGGITVFN
                     PIVQFDVKPTDKVAVIGIGGLGHIALQFLNAWGCEVTAFTSSESKRHEALAIGAHKTL
                     NSRDHGEIESAAGTFDFIISTVNVKLDWNLYVNTLKPKGRLHFVGATLEPLDLGAFSL
                     ITGQRSISGSPVGSPATIESMLEFARLHQVKPLVETYPMSKINDAFDHLKSGNAKYRI
                     VLSNR"
     gene            complement(18825..19232)
                     /locus_tag="PLD53_01000"
     CDS             complement(18825..19232)
                     /locus_tag="PLD53_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRC59510.1"
                     /translation="MTKSTTTYWNALHPENRERWTPIKGMEGKAEELTLSIDQATGEY
                     TRLTRFLPGADTTEFGGKTHPYPEEVFIVSGRLYDEAFDLWLQSGHYASRPPGELHGP
                     FKTDVGCVVLEVSFPNRTGAGDVAQAIVPPHTT"
     gene            complement(19386..19850)
                     /locus_tag="PLD53_01005"
     CDS             complement(19386..19850)
                     /locus_tag="PLD53_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59511.1"
                     /translation="MRSEISRRAREVFHSDALAAPRMTLRGGYAVDSYDFAPPPDPVL
                     DKPTDTYLETYTFWGLAHLDPASWRHYLPHLINYALRHMDDPEMAVEGLLHSLRPPDR
                     EPPRLASLTAEQEAVIVALLEEVAFSEDSANSDFAMQVLEEWWIPIALYRQR"
     gene            complement(19875..20198)
                     /locus_tag="PLD53_01010"
     CDS             complement(19875..20198)
                     /locus_tag="PLD53_01010"
                     /inference="COORDINATES: protein motif:HMM:NF026918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 53 family protein"
                     /protein_id="HRC59512.1"
                     /translation="MNTLARLQTWYLAQCNGEWEHSSGVNIQSCDNPGWWVTINLTGT
                     ALQTQQFAEIAEGVDDLRFALGSHWLSCYIENGTWHGAGDETMLERILEVFLGWAEDN
                     VGSLE"
     gene            complement(20349..>20509)
                     /locus_tag="PLD53_01015"
     CDS             complement(20349..>20509)
                     /locus_tag="PLD53_01015"
                     /inference="COORDINATES: protein motif:HMM:NF027067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC59513.1"
                     /translation="SGLSQTAFAALLGVSVRTLQDWEQGRREPSGAAKTLLRVAERHP
                     EVLQELAA"
BASE COUNT         4236 a         5590 c         6148 g         4535 t
ORIGIN      
        1 caacaggatt cgtttgtcgc gggtttgctg gattgcccgc actatacgcg gcctgaggaa
       61 tacgagggtc agcgggtgcc ggatgtgttg ttgtccgggc accacgagca gatacgtcgc
      121 tggcgcctga aacaggcgct tggccgaacg tggcaacgac gccccgagtt gttggcaggg
      181 cgatcattgc cgaaagagga aacgcaactc ctggtgcagt ttcaggagca atgcggtcag
      241 gaaaataaaa cgtagtttgc agaacggagt ttaaaaaatg aatctgatcc agcaacttga
      301 gcaagaagaa atcgcccgta tgggcaagac cattcccgcg tttgcgccgg gcgacaccgt
      361 cgtcgttcag gtgaaagtga aggaaggtac ccgcgagcgt ctgcaggctt acgaaggcgt
      421 tgttatcgcc aagcgtaacc gcggtctcaa ttcaaacttc atcgtgcgca agatttcgtc
      481 cggtgaaggt gtcgagcgga cgttccagac ctattcgccg ctggttgcgg cgattgaggt
      541 caagcgccgc ggtgatgtgc gtcgtgccaa gctgtactac cttcgcgagc gttcgggcaa
      601 gtcggcgcgc atcaaggaaa agctgacgcg caagcgcaag gtctgattaa caaatacaga
      661 tacccaaaaa ccatgaacgg ggcggagtcg aaagacttcg cccttttgct ttgcggcggt
      721 tgccggactc agacgggttc ggggttgcgg ttttcgatca atgcgtaggc cgaatgattg
      781 tggatcgact cgaagttttc ggattcaacc acgtaagaat agatgcgggg ttcggcgctc
      841 aggcgcgccg caacgtcgcg aaccatatcc tcaacaaact tagggttgtc gtaggcgcgt
      901 tcggtgacgt gcttttcgtc cgggcgcttg agtagtccgt agagctcgca ggaggcttcg
      961 gcttcgacaa gctgaactat ctcttcaatc cataggtgat cgttgatcct ggccgtcacg
     1021 gtaacgtgtg agcgctggtt atgtgcgccg cgttccgata ttttctttga gcaggggcaa
     1081 agactggtta ccggcacgac gaccttgatc gtcgaagcga tttcgccatg gcagatctcg
     1141 ccggtcagcg tgacatcgta atccatcagg ctctgtacgc cagacaccgg cgccgccttg
     1201 ttgatgaaat acggaaaatt catctcgata tgcccggttt ccgcctccag tttttcgagc
     1261 atttcgcgca gcatggtcgg gaaattctcg acggagattt cgcgttcgtg gctattgaga
     1321 atctccacga agcgcgacat gtgcgtgccc ttgaaattgt gcggaagacc aacgtacata
     1381 ttgaacatgg cgatggtatg ctgcacgccg cccgacttgt cgagtacctt gaccgggtga
     1441 cggattgact tgatcccaac ccggttgatg gcaatctgac gggtgtctgc caggttctga
     1501 acatcgacca tggcgttcgg gattggagct tgaggtgcgt tcattgcagt tcctgatgag
     1561 cggtggatat cgcccctgat ttttgatctg aagaaggcgc gctgccttga tgcacgcgca
     1621 ccgtacgaac aatgccctcc ttgtccagtc cggcttccgc caatagcagt gcctgatcgc
     1681 cgtgatcgat gaaatggtcg ggaaggccaa ggcgtatcag tttcgccgga ctgtcaattt
     1741 cgtccagcac tctcgcgacc tctgcacccg cgccaccgat cagggcattc tcttcgacgc
     1801 tgatcagcag ggaatgttcc cgcgccagtt caattatcag gtcgcggtca atgggtttga
     1861 cgaaacgcat gttggcaacg gtggcatcaa tctctggcgc agcgcccagt gcgggcacga
     1921 gcatgctgcc aaaagcgagc agggcaacac ccttgccgcg gcggcgtatt tctcccttgc
     1981 cgatgggcag gtcgagaagc gctttatccg tggcaacgcc cggacccgag ccgcgcgggt
     2041 agcgaacggc acttgggccg tcaatgtgga acgccgtagt caacatgttc ctgcactcca
     2101 tttcgtcggc gggcgtcatc accaccatat tcggaatgca ggtcaggaaa gacaggtcga
     2161 aggtgccgtg gtgtgtgggg ccgtccgccc cgaccagtcc tccgcggtcg agtgcaaaaa
     2221 ccacgggtag cttctgcaag gcgacatcat gaatcagctg gtcataacct ctctgcagga
     2281 aggtcgagta gatcgccagc accgggcgca tcccttcgca agcgagtcca gcggcaaagg
     2341 tgacagcgtg ctgctcggcg attccgacat cgaaatagcg ttcgggaaaa cgttcggcaa
     2401 accgtatcat gccggatccc tcacccatgg ccggagtaat gccaatcaac ctggggtcgg
     2461 ctgcggccat gtcgcacagc cattcgccaa atatctgggt gtaactgggt ttgccgcccg
     2521 ccttgccgtt ctcaataccc acttcaggat ggaatttcgc cacaccgtgg tagaggatcg
     2581 gatcagcctc ggccagcttg tatccttgcc ctttgcgggt aatgacatgc aggaattgtg
     2641 gtcctttgag cttctggagg ttctgcaggg tgggtaccag ggaatccaga tcgtggccgt
     2701 caatcggacc gatatagttg aagccgagtt cttcgaatag cgtgcccggc gtcagcatgc
     2761 ccttgacgtg ttcttcaacc cggttggcga actccagcag cgaaggggaa acgcgcagca
     2821 ctttctcacc cgtttttcga accgagttga aggtgctgcc ggaaaacagg cgggccaagt
     2881 acttgttgag tgcgccgacg ggtggggaaa tcgacatgtc gttgtcgttg aggatgacga
     2941 gcatgtcggc atcggctacg ccggcgttgt tcatggcttc gaaggcctgg ccggccgaca
     3001 tcgcgccgtc accaatgatg gctaccgtct ggcgggcttc gcctttgagc ttgacggcta
     3061 gtgccatgcc gagcgcagca gagatcgagg tggatgagtg tccgacgccg aacgtatcgt
     3121 agtcactttc gctgcgtttt gggaaacccg aaacacctcc gtgtgtccgt agcctggaca
     3181 tgcccgcccg acgcccggta agtactttgt gggcataggt ctggtgaccc acatcccaga
     3241 ccaggcggtc atgcggtgtg tcatagacat agtgcagcgc gacggtcaat tcaacggtgc
     3301 caagattcga cgagaggtgg cccccggtcc tggacacgga ttcaacgagg aagctgcgca
     3361 actcttcggc aagctggggt aactgctttc gatccagtgc gcgtagttgc gccgggttgc
     3421 tgatggtgtc gagcagtgga taggaggtca tttgtttgct gtatccggca tgaaccgcga
     3481 tctctggtca gaatatgagg ccagcctcgt cggctaaaac ttgcggtggg tgatgaacgc
     3541 ggtcagctca atcaggcgtt gaccgcgttc accaaaaatg gcgagtgaat caagagcctg
     3601 ttttccaagc tcatcggcga attcgcgggc gcgttccagt ccaagcaggc tgacataggt
     3661 cggtttttca tctaccgcat ccttgccggc cgttttgcca agggtggccg tgctcaccgt
     3721 acagtcgagg atgtcatcaa cgacctggaa cagcagccct gcgcgcttgg caaagcgatc
     3781 aagcgattcc ctggcttcgt ccgatagcgt ttctccgcac agggcaccaa gcattacagc
     3841 tgcacggatc agtgccccgg tcttgagggc atgcatcagt tcgagctctg gttgatccag
     3901 gacgtttcct accgcgccga gatcgatggc ctgaccgccg gccatcccac tggagccgct
     3961 ggcgtgtgcc agcagtcgaa ccatttcaag ctgacgtgcc gagttggcag attcgctgcc
     4021 taaatccgtg cgggcgagga gctcaaaggc cagcgactgc aaactatcgc caaccagcag
     4081 tgccgttggt tcatcgtatt cgacatggca ggtcggtcgg cctcgacgta gcacatcgtt
     4141 gtccatacac ggcaggtcgt cgtgcaccag tgagtaagcg tggatcagct caaccgcact
     4201 ggagacgatc tccagtttct ccggcgaacc accggtcagt tcaccggcag caaaagcaaa
     4261 cagggggcga acacgttttc ccccgtttag gctcgcatag cgcatggcct gatgcaggcg
     4321 cgcgggaatc gcttccgcag gcggaagcag gcgtgacagc gtggcctcta tccgagtttg
     4381 caccgtgcgc atccagtccg ggaaggggag ctctcgggtc ttgcctcggg gaaagggaat
     4441 tgcgctcact gtgcttcacc gccggcagtg tcaaactccc gtagcgaacc gtcttccaga
     4501 acctgaattt tgcgttcggc atcactgagc tgctgctggc agtacttgag caactcgcag
     4561 ccgcgtcggt aggccgctat tgactcttcg agtggtaact gcccagcttc catattctgg
     4621 acgatctgtt ccagctcggc cacggcggct tcatacttca tttcgctcgc cgcaggcgtc
     4681 gctggctggg aggcaccact ttcatgcgga ttggcagggt tggttcgtac catgggtaaa
     4741 ggtttgatca aactcagcaa aatagctcaa gaaggggctt ccggtcaaat ggaaaccgac
     4801 cgtatcctcg ttccccaggc actactcagt gctgcttacc ggctgcaagc ctcgtttgcc
     4861 aatccgagtg attgatgccg catcgttctc aagcgaatcc tgccaagcag gactgatgct
     4921 acactcctgt gaacacaagg ggagacagcc atgattgtta tccagcacca gcccaaacgc
     4981 gtcgaagtca atgtttacgg ggaatttacg cttgcggact ataaagaatt cgaggacatg
     5041 gtcaactaca aggtcaaatt cgagggaccg gtcgatctat attttgatct gcgtgaaatg
     5101 gctgatttta cgcttgatgt ggcctgggag gatattgtgt tctcacgtgc gcatgccaaa
     5161 gattttagcc gtatcgcggt cctgacgcta agtcagtggg tggcgtggag cgcatggttg
     5221 tcgcagatat ttgtccgtgc cgaaatgcgg ctgtttgacg acgaagccga ggcacgggcc
     5281 tggctggatg agtccatttg aggcattgtg gcggcgtgaa tttcttgact ttactgtcct
     5341 gcgatgaact ttccaggcac ttgaatcgtc cggactggcg ggtgttcgat tgccgtcatt
     5401 cgttgtcgga tgtggaatct ggcgagaaag catacgccga ggggcatgtg cctggagcgt
     5461 tcttcctgca tcttgaccgt gatctgtccg gcccgatgaa tggccggaat ggacgtcatc
     5521 ccttgcccga tccgtgttgt cttgcgagca ccctgggcgc tactggcgtt gcccgggaca
     5581 cgcaggtcgt agcctatgat gacgctggtg gcatgatagc ggggcggttg tggtggttgt
     5641 tgcgctggtt gggacatgat cgtgttgccc tcctcgatgg cggcatcaac cagtggatga
     5701 aggaagggcg ggaactgtca accgaattga cacagtcggt tccatgcgtc tttgacatag
     5761 cccgacgcga ttgggtcgtc agcactgaat cggtgcttgc caatatcaac aaagaaggct
     5821 tttgcctgat tgatgcacgt agtccggatc gtttccgtgg tgagaatgaa acgctcgacc
     5881 ctgtcggagg ccatattcct ggggcacgca accgtttctt tcgtgacaac ctggatgaca
     5941 aaggctgttt ccgccccgcg gctgagttac gccgcgattt ccttgagctg cttgctggag
     6001 tagaggctga caaggccgtt atgcaatgcg gttcaggggt caccgcctgc cacaatctgc
     6061 tggcgatgga aatcgccggg ctgcacgggg cgaggctcta tgccggatcc tggagcgagt
     6121 ggtgtgctga tccagcccgg ccggttgagc gctgacaagc cactgctctg aagtgaacgg
     6181 gaaaacggca tttcacccga gcttgagatc actattcccg gccggaatct agcgggcgtt
     6241 catcgactca agagcgccac ttaaggctcg ttcgaagatc ggttcgtcac ggacgattgt
     6301 cgctctgccg tcgcgaatct ggcgggccag cgcttcggga gcaatcgaaa tggccttggc
     6361 cgagcgatgg ttgctgaaaa gcagaattcc ccgctgcggg ctgatccagt tgaggcgttc
     6421 ccggttggta cttgccacgt cgccgtctgg tccttcgagt tcgtggaact ccacccagtc
     6481 gccacgcttg agttcgctga ccaagcgaaa gatttcacga tcatcggcac gccagattgg
     6541 actcgcgccg accagaatta cctcttcgac ctcgacgcca ttgtcggtgc tgcgcgttac
     6601 cagcaagtcc ccttcttctg atgggctaaa ggcgggcgat gcattttccg ctccggatgc
     6661 cggtggaatc tccggcgttg gagttgcgtg ggtttcgcct ttgagtgccg cggagtggca
     6721 ttgcaccagt tgatcaaaga aatcactgcg ctgggacggg ctggcatcga cactgtccag
     6781 acctttgttg atctgggcca gtagcttggg cagcatgccg atcagtttca ggcgatcatc
     6841 aggcgtcttc ttggcctcga tactccagga cagatccttc atcgcttctt tggccgtttg
     6901 ccagtcagtt tcattcgggt cttctctgac ggcaatctta tgcagcacgc caacccagtg
     6961 ttctgttaga aacgcgtcga tgagcgctgg aggcgaattg ctggcccgga aagcctgtac
     7021 ggcacgttgt gcacgctccc acccttcggc gtcctgttcg cgaagaatca caatattggc
     7081 ggctgtcaga gccgtgctgt cttcttcaac ctggcactca tcgacgaaag cctcaagttc
     7141 agcgagtgcc gtcccgaaca cctcgacatc gtcttcgaaa tcggcttgga ttcgttcgac
     7201 gagttcggcg agcttgcagt agaacggatc ggtttcgtcg accagactgc cccagcgaat
     7261 ggatacgccg gagacgctgc caaggaatcg gcgcgtcgga tgctgacggt cggcgaagaa
     7321 gcccgggttg agcatcgcca ctttgagaac gggaatctgg agccggctga tcaatgcctt
     7381 gacggcaact ggaatatgcg catcgtcgaa aatgaaatcg aaaagcatgg cgacgatgtc
     7441 gattgttact gcttcgagtc ccccgacctg ctgcgcgctt tccgattcgc gtaccagccg
     7501 cacctgattg acaatcagac caccgggctc gcacgatggt gcatgctgca ggtcgttgag
     7561 cgaggaaaag agtagtcgac tgacgctttc tggatcaagc acttctcctg gcggaccgga
     7621 tgtcatcgcg ccgggcatgg cgttcccgcc atcgctggaa agccccgcag gcagccgggc
     7681 gacgcgcgct tgtgcgaggc gttgcaaagc atcgagaatg cccgcacttg agttcaaccc
     7741 agaagttgtt ggactcgctg gcgtcatact cgcgggcgac gagctatctg gttcggaagt
     7801 cgctgacttg gcggcagatc cgctacttct ccggtagttc cgtttgagat ctggcaggat
     7861 gccccgttcg accaggtaag cattgatttg ctgatagatc ggcggcagag caaggtgcag
     7921 atgccgttcc aggcggcgca ataggagcag tctctcctcg gcattcgagc cgattgtagc
     7981 gcaggcgtca gagagcgcac ggcaaacgac gggtggggcg agcgggttgt cgctctcggt
     8041 cagttcgtca cgcccgagca agaccgcaac gcgtcggtta agcgcataca gttcttcgtc
     8101 gcatgtctcg gaaagctgcg cagaaaactc gcgaatgacg atgtgttcgg agaggtcgtc
     8161 gtcggcaacc aactgcagac cttcaaagtc gggaatttcg agcgaggctt cgcgcttgcc
     8221 atctttttcg gcttcctgat tctggcagcg gtcagcctca gcggaaaggt cgcggcgaaa
     8281 ggtttcaacc agatgcgacc agtccttttc aacatcagcc cgcaggtcaa gataacgggt
     8341 ctgttgcagc cgttcccttg ttgaatccgc caggagaaaa agctcttccg agactggcgc
     8401 ggcaacctca cgcagccaaa caaacaggct attgaccaac tgatcccggc attcagcgag
     8461 aatgcgacgg cctgggctgc gatcccgccc cagactgttt agcgagatta ccctgtttgc
     8521 ggccgggctc gaagaggatt gggtcacgat atcgccctca ttgaaaagcc gcctgctgcc
     8581 aggcggacaa aatcaacagg gcggtcggac gcgatgtatt gcaggtttgg ttgtactggc
     8641 aacgctgaat cacgatcaac cccgctttct ggtagtcccg ctgccgttgc cgttatgatg
     8701 cggcggtagg ccggtaactt tgcgacgccg tctttcgacg ggtttgcctt gtttcagagg
     8761 ccggtgtgtt gctccgacca cggtcaatgg tatcgtccga aaatcgagcc agaagagact
     8821 caagttctac gctcatccca tgctcgttgc gatactgctt gtcgactgaa taatatcgga
     8881 aaaccgaagg aaatcaagag ctatcggaat gtatttgcgc cagtgcccaa cggacgtgtt
     8941 cgcgaaccag tgctgaagga tgttccaggc gtgtactgag tgcagcagac gcttctgccg
     9001 tatttgctgc gttgccaagg gcgacggcaa tgttacgcag ccagcgcgta tgaccgatgc
     9061 ggcgaattgg gctcccctcc aggcgggtca tgaatgcctg ttcgttccag gagaagagtg
     9121 tggcgaggga tgtcgcatca agcccgtttc gcacggcaaa atcggggtcg ccgagctgtg
     9181 caaattcgtt ccacggacaa cagagctggc agtcgtcaca gccatagatg cgattgccga
     9241 tcaggggtcg caattcgaca gggattgcgc cagccaattc gatggtcagg tacgagatgc
     9301 agcggcgggc gtcgacttcg tagggcgcga cgatggcggc agtcgggcag gcttcaaggc
     9361 atcggatgca ggtaccgcag tgttcgctga tcagttcgtc cggcggaaga ggtagcgtgg
     9421 tgtagatctc gccgaggaaa tgccaggatc cactccgggt cagcgccagg ctgtgtttcc
     9481 cgcgccaggc aattccagac tgacgcgcga attcgacttc catgaccggg gctgaatcgg
     9541 aaaatacgcg gtaagcaaaa cggctttctt gctccgactg taaaatttcg gcatggatcc
     9601 gattggccag tttctgcagc cggttacgaa ttgtcttgtg atagtcgcgg ccaagcgcat
     9661 agcgcgaaat gtaggccagt tcgcggttgc caagcacctg ctcgcattcg gctgccgccg
     9721 gtaaataggg aaggcgagcc gtcacaacgg atagcgttcc gggcaccagt gtctccggct
     9781 gggcgcgcaa cgcggcgtgt ttggccatat aattcatttc gccgtggcgg ccaagttcaa
     9841 gccagcgtag cagtttcggt gtcgcggttg aaacgtcggc tccagcgatg ccgagctcgg
     9901 aaaagcccag ctctttcccc cagtttttga tttttttgac cagcgcgaca aaatcaaaac
     9961 cgttccaggc gcttgccgtc agagtgcgtg tatcggagtt gaactcttgc atagcccgaa
    10021 tcatagtcct gttgtcggtt ccgatcgtga tctatcgacc gtgaccatgt atttgccgga
    10081 cgaggcgacg accatggtcc tggggcgtga actggcgcca ttgctggcgc ctggcatggt
    10141 cgtctggctg aacggtgatc ttggctccgg caagacggcg cttgttcgcg ccctgttgcg
    10201 agggcttggc cacgtcgggc cagtcaaaag ccctacctac acactggttg aagtttacgt
    10261 agtttcgagc ttatactggt atcactttga tttttatcga atcagcgagc cagaagagtt
    10321 tgaagatgcc ggacttggtg aatactttcg cgatgatgca gtgtgcttgg tcgagtggcc
    10381 cgacaaagcg gcagggtatg tgccgccagc cgatctgtcc attatgcttc gctttgcagc
    10441 gggccaaccg gaatcgggcc gcgatctcga aatcgttgcg cacagcgagg ctgggcagcg
    10501 atgtctaacc gcactcatca accgcatgcc gcgcgtcggg agctgatcaa gttcgcaggt
    10561 gcgtcgctgg tactgttggt cagcccggtc ggtcgtgccg cggttaatcg taaaccaagc
    10621 gttcttgccg ttcgcgtctg gccggctgat gactacacgc gtgtggccat cgaatgcgat
    10681 acgccgctga aattcactca cttcagcgtc aagaatccgg accgactggt cgttgatctc
    10741 gaaggcatcg agttcaacag cgtccttcaa gcgctgggca acaaggttgc ggcggacgat
    10801 ccgaacatca agctgttacg tgccggacgt ttcaagccgg gtgttgtgcg tctggtcatg
    10861 gaactcaaga acgaagtcaa tcctcaggta ttcgagctgc ccccggcggg agaatacggg
    10921 catcgtcttg tgcttgatgt ttacccgctt gaatccccgg atccgttact ctctctgctt
    10981 cagaagagcg aaccatcgcc cgccagcccg ggcgaccggc ttgaggacaa agcggtacag
    11041 aatgccgaaa acaaggcaag ccggtcacgg gagggtaggc ccgtggtgga tcgcctgata
    11101 acaattacgc tcgatcccgg gcacggcggt gaagatccgg gtgccgttgg gcgtggcggc
    11161 agttacgaaa agcatgtcac gctggcagta gccaaacgcc tgaaagcgaa gatcgatgcc
    11221 gagccgaaca tgcgcgcggt attgacgcgc aatacagact attttgtgcc tttgcacgtg
    11281 cgggtacaga aagcgcgccg cattcgctcc gatctgttcg tatcgataca cgccgatgcc
    11341 ttcatccgac cggatgctca cggttcgtct gtcttcgcct tgtccgagag cggtgcatcg
    11401 agttctgcgg cacgctacct ggcgcaaaag gaaaatgcct ccgatctgat tggcggcgtc
    11461 aatctcgatg tcaaggatcc gatgctggca cgcacgctgc tcgatctgtc gcaaacggcc
    11521 acgatcagcg atagtctgaa actcggcaaa gcggtactcg gggaaattgg tggcatcaac
    11581 accttgcaca aagccagcgt cgagcaggcg ggctttgccg tactcaaggc gcctgatatt
    11641 ccgtccatcc ttatcgaaac ggcctttatc tcgaatccgc aggaagaaag gcgtctgaac
    11701 gacgaagcgt accaagacaa aatggccgag gctatcattt cgggcattcg aaaatacttc
    11761 gccaagaacc cgccgctggc gaagtcgaaa ctggcgagca ttgattgacg gcgggtacgc
    11821 gtacatcatc tttctccgct gtgtcccgat agcccgatca ttccttttcc actggttgat
    11881 tcaagtcaga agtgcgattc caaggctaag gtgaaagttc agcgacagaa tcagtcgccg
    11941 ccattggcgg cctggccggc aaggtagttg ttcttgacgc gcaggtagtg ctctgcggag
    12001 tagcggagat aggccagttc ggaagtggat aacgggcgta cccgttttgc tggccgcccc
    12061 acataaagga acccgctttc cagatgcatc ccgggcgaaa ccaggctgcc agcgccgagc
    12121 atgacattgc tggaaatctt ggcgtcgtcc atgacaatcg agccgaggcc gatgagacag
    12181 tcattgccga tttcacaacc gtgcagaacc gccgagtgcc cgacggttac atagtcaccg
    12241 atgatcagcg gtgagccaag gggcttgttc tcgttcttgt gcgatacatg gcacatcaca
    12301 aaatcctgga tgttggtgca gcgcccgatg acaatatgat tgacatcccc acgtatgacg
    12361 gtgttgcacc aaacggatac gtcatcgcca aggcgaacgt ctccaatgac ttgtgacgat
    12421 tcgtggagga agattctttc ggccagttgg ggcgatgtgt ccagatagtt tgagattggc
    12481 atggtgcagg atcaggtgag ggtaaaagaa atatgcacat caactgcaaa aactgcagca
    12541 agttgcgtgg gaacattatc gccccaggaa tcgatctcgc caaggcctga tagcaggttg
    12601 cgcgaattct gaagctagca ctcttcggtg ctgccgttga cccagcgaag cgattcgacc
    12661 agacagctgt taagcgtgcc caaactcagg ccggcttcgg gcaactggtc aagcatgtgc
    12721 atgcagcctt ggctatcgtc tgcgtcgaaa cattcaagaa gggccagggt tttgccgaga
    12781 acccccgcat gcgaggtaag cgcctccatg accgaggttt caagtgcgat ttgctcgaat
    12841 atttcgtgca ttggcaggcc cagcgcggtg ggcatcatcg acatgatgcc ggtaatgaag
    12901 gcttgatcag ccagcgtacc gttacgcgga tagctatgtc taagcaataa ttccatcatg
    12961 cgcccacgca gcgcagcgac ctgcagcagc ggggaactgc tggggtcggg tgtttcctct
    13021 gccggggtca tgagtagcag ttgcagccag cgttgcaatt ggcgcctgcc cagcagggta
    13081 atggcgtggc gcagggaact gatgcgacgg ttcagcccgt agccaaccga gttgacgatg
    13141 cgcaacaggt ttgcggcgag cgccggctcg tgtttgaatc cgtcttcgat cttggcggta
    13201 tccagatcgc ggctgacgag attgatcagt tgaatcaggc tgatctgtga cgccatgaga
    13261 cggcgtccgc tgacgatctc cggacgggcg aaatagtacc cctgaaaaag ctcgaatccg
    13321 acctttttgg agagttccat cgcttcctgc gtctccacgc cctgggcaag cagcttgagc
    13381 gggagtttgg ccagagggcc ggcgagttca cgcagcttgt tttcatcgtg ttcacggatg
    13441 tcgatcttga tcacgtcgag catgttcagc aaaggcatgc tgcgctcatc caaaccagca
    13501 taattggcca gcgccagcga atagtctcgc tcgcgcagcg cgcggcagcg tgcgagtgtt
    13561 tgtgcatccg gtgctgtctc gagagctagt tcgagaatga cggcgtcggg tggcattgcc
    13621 tcgatcgcat cttcatgaaa aaacatcggg tcaaagcgca ggaaggcgat gttgcgcccc
    13681 agtgcactgc gtattcccag ctcggcgtaa gctgcgcaaa taagcattgc cgcccgtgaa
    13741 gggttgtagg tgggtggggt cttgtcgccc gcctccaact gcaacataag ctcgtaaccg
    13801 acaagttttt ggtgctggtc gaggaccggc tgtcggccga gaaggagctc gttattcacg
    13861 gtatcgtctg ggagattgga gccagcgaaa atggctattg ttcgacggct gttgtgtcct
    13921 gctcctggcc gaggttgttg gcccaggaga atgcccgggc gagacacttg ccgagaaagt
    13981 ccaggttgat tgcaggaaca cggtgcagcg cttcttcaag ctcgaccggg tcggtcactt
    14041 cggtggcggc agcgataagc agaagctgtc caagctggcc gctctgttcg ggctgctcga
    14101 tcagggcttg cttgacgcgc tgggcgacgg gtagctgggc gagtatgtcc accatcgtca
    14161 tgccgagcag ggtaggcatc agcgacatga tgccgaccat gaacgcatga tcggaaaact
    14221 cgcggttgcg cggttgcaca cggtcggcca gcaactcgat cagccgtccg cgggttgcgg
    14281 ccagttgcag tagcgggttg atgccctgag cattcccttg ggggttggtg tagatcagga
    14341 gctgcaacca gcgctgcaac tgtcgtcgtc ccagaatcgt aatcgcgtgc cttagcgagg
    14401 tgattttcgt cgacagaccg caactgaccg agttggtcag gcgcagcatg ttgacggtga
    14461 gccccggttc gaccttgaag gcgttttcaa tctcgctggt gtcggcgtcg tccatgatca
    14521 tgcccagcag gcgaagcagc gcaacttgcg tcggattgag ttttttgccg acgatgattg
    14581 tcggttgtgc gaaatagtag ccctgaaaca gatcgaagcc gaggctttgg cactgggaat
    14641 gctgtttgac tgtttccacc ttttcggcga gcagcttctt gccgaatttc ttgagctgcg
    14701 taacgagggg cggaagccgt tcggcatcca gtttcaggat gtcgaccttg atgatgtcgg
    14761 tcaattcgag cagcggacga taggcttcct cgatctgggc aatgtcatcg accgccagca
    14821 tgaatccctt gtcctgcagt tgatggcaac gtgcaaccac ttcgggcgtc ggactgatgc
    14881 tttcgagaat ctcaagaacg acgagttgcg gcggcagcac ttcgatcagc tcgctgaaca
    14941 ggaggtcgct atcaatgttg atgtaaccgg tatagggtcc gagcgccgca tgagccgcca
    15001 gttcggtaaa ggcgttggtg atgacagtcg cggtcgcctg agtcggatcg gtgatatcgg
    15061 cgcttccgtt cgcggctgga acgccatcgc ggaaaagcaa ttcgtaggca atcagttgct
    15121 gttcgcgacc gagaatcggt tggcggccga ggaatatcag cggactttgg gtttcagaca
    15181 tctgtcatca gattgttttt gtgaggtctc tggacgaaaa tttgtcgagt gaaagatgcc
    15241 gaacgtttgc gccgcaactc aggacgattc acgcgcaaga ttattggccc aggcgagtgc
    15301 ccgggtcagg cagccattgg caaatttcgc gtcgattccg gtgatgccgt gcagcaagtg
    15361 ggccgcattt gcaccaccgt catcctcttc cagcgattcg acaaggccga gcagatcgcc
    15421 caaggtcccg cttcgactgc cgagcgcatc caggacaggc tgggcgacag gcaactgctt
    15481 gagaatgtcg gacatgttgt gaccgaccag tgtcggcatc agggagagaa tgccgaccat
    15541 gtaagcctga tcgatgagct cctggccgcc agccttggct cgcggtgtcc tttcgaccag
    15601 caactccatg agccgtccgc gtgtggcggc cagttgcaac agcggattga ccgcctgtct
    15661 ttcgcccgaa atgccgctgt agagcaggag ttgcagccag cgcagcaatt gccgccgccc
    15721 gagcagggtg atggcgtgcc gcagtgacgt gatttttgtg gccatcccgg cgccgaccga
    15781 gttggtgagg cgcagcagat tgaacgtcag catgggttcc tgtttgaaca cgatctcgat
    15841 ttcggcattg tctgcatcct ggctgagcag tccgagcagg cgcagcagcg agagttcaga
    15901 ggtgtgcagg ctcttgccct tgatcacggt ggggtgggca aagtaatagc cctggaagag
    15961 atcgaagccc aggccatggc aaagcagcat ctgctcattg ctttccactt tctcggcgag
    16021 aatcgttttt tccagcggtt tgagatttgt ggacagttgc ttcagccggg tcgggtcaac
    16081 acggctgata tcgaccttga tgacttcggc aagcttcagc aaggggcgcg actggtcgag
    16141 tgattccggc cgttcgcgga tggccaggac gaagccttcg ctacggagca gttggcagcg
    16201 cgccagcatt tcatccgtcg gggctaccgt ttcgaggatt tcgagcacga cggaatgcgt
    16261 cggcaaggcg tagatcaggt cgctgaaaag gagtccatgg tcgaccttga tgaatccctg
    16321 atacgggccg agcgcgtcgg ccatcgagaa ctcggtaaat gcattggtaa tgacggtggc
    16381 cgtggcctga ttgccatcga caaaactggc gaagttcccg ctctcagcac tgccgctacg
    16441 gaagagcagc tcataggcaa acagttgctg gttgcgatcc aggatgggct gccgacctag
    16501 gaagacttcc tgcaggtcat tgtcgggcat tttctgttcc tcgctgaaat tcgggaaagg
    16561 ccttgtcgtt atttgcaaat cttagcacta cccaacgccg ggtgtacagc tacaagcgac
    16621 agattgcggc gcttgatcgg agaaacggac aaatacagga ggcaatgcaa tatgttgtgg
    16681 cgcggacaga gcgtctcatt ggaaatgcat atgattactg gctgaagctc atcgtggttg
    16741 gccaagaggc catgcagggt gaacggcagc cgcgatcatt ccggattgtt atcccgatcg
    16801 atgcaaccga acgggcttat cgaagtgcgc tactcgcgct atcggcgcgg agctgacctt
    16861 cgcgggacac ggtagcgggc gtgcagcgtt ggccgtcagc gggggcaggc caaatcggcg
    16921 agtctcgacc actatgtgcc gtgcggcggc tcgctggcgg ttgcgggaga gcaaaaccac
    16981 agcttgttgc cttcactgtc cagaaaactg ccgacgtaga aatcgccata gacctgcact
    17041 tcgttggcga ttgtgatacc gcgctggcgt aatgcgtcct gcgttgccgg ggcatgctcc
    17101 gtggcgagcg taacgcgtgg gttcggcgtg tctgtcggcg ccgcccagtc cggtttgagc
    17161 atcaagatca cttggccgag ccaccatcca acctgttcgg ttccctcgaa cgctggctcc
    17221 aggcccatct tttccttgta gaatcgattc gcgcggtcaa ggtttgtcac tgaaaccgcg
    17281 acgactttga cgcgctggat gccgagcggt ggcagtactt cgttcatgat ttttcccctc
    17341 acgattggtc atcaatgggc tgctgtcagt ggacaatcca aagccggcta aattcctgct
    17401 tttcagagcg ccatcggaga catcgggaat agttattctt cttacagata aatatgagaa
    17461 atgcgcaatt tgaatcgcgg ccttcgccgg actcaacggg cgagaatgag agcattcggt
    17521 cgcattgttg accgccatct ggcgctgtac gactccggca atctctttgg catcacaggc
    17581 gcgcaattgc tttccaccat catccaagca gattcttgac cgggcggaaa tttcggagag
    17641 cagaaccgga ccttcaacgg caacggtcag cggtgcccgg gggactcaga acacgacttt
    17701 ctcttgcagg ctgattccta tctgtttgag agcacgatcc tgtattttgc attgccggac
    17761 ttcaggtgat caaaggcgtc gttgatcttg ctcatcggat acgtctcaac caagggtttg
    17821 acttgatgca gccgggcaaa ttccagcatc gactcgatag tcgccgggct gccaaccggt
    17881 gatcccgaaa tcgagcgttg tccagtgatc aaagagaaag cgcccagatc gagaggctcc
    17941 agtgtcgccc cgacaaaatg caggcgtccc ttcggtttca gtgtgttgac gtacaagttc
    18001 cagtccagct tcacgttgac cgtggaaata atgaaatcaa aggtgccggc agcggattcg
    18061 atctctccgt gatcgcgcga attcaatgtc ttgtgcgcac cgatcgccag ggcttcgtgt
    18121 cgcttcgact cgcttgaggt aaaagccgtg acctcgcatc cccaggcatt gagaaattgc
    18181 aatgcgatat ggcccagtcc gccgatgccg atgacagcca ccttgtcggt gggtttcacg
    18241 tcaaattgca cgatcgggtt gaacacggtg atgccgccac agaacagcgg accggccgac
    18301 tcaagatcga tcccttcggg aattgcaatt acgctggagg catccgcgcg caccttatcg
    18361 gcaaagccgc cgtgatgcgc aacaatggtc gattgggctt ctgcgcaaag attctgatct
    18421 ccggttttgc aggaatgaca gtggccgcag tagccgcagt gccaacccag cccgacgacc
    18481 gttccttcct ccaggttcag gacatggctc cctctggcgg aaatccttcc gatcacctcg
    18541 tggccggcca ccagagggta ctttgacatt ccccactcgt tgtccaccat actcaaatcg
    18601 ctatggcata tcccgcagtg aagaacgtcg atttccactt cgtcgggatg gagttcgccg
    18661 acttcatagc taaaaggctc aaatcgaccg ccggcttcga gtacggcata ggcattgatg
    18721 gtccttgttg tgctcatggc atttccgtcc ttgagttgga gttggtgggt gttgcttcga
    18781 aaacctcttt gcagaaaggg gccaaccgga gctacacggc actatcacgt tgtgtgcggt
    18841 ggaacgattg cttgggcgac atcgccggca ccggtgcgat tgggaaatga tacttccagg
    18901 acaacacacc cgacatcagt cttgaacggg ccgtgcagtt ctccgggagg ccgactggcg
    18961 taatgcccgg actgcagcca gagatcgaac gcttcgtcgt agaggcgccc gctgacgatg
    19021 aatacctctt cagggtaggg gtgggtcttg ccgccaaatt ctgtcgtatc ggctccaggt
    19081 agaaagcggg tcagcctcgt gtactcacct gttgcctggt cgatgctcag agtcagttct
    19141 tctgctttgc cctccatacc tttgatcggc gtccaacgtt cccgattctc gggatgcaag
    19201 gcattccagt atgtggtggt ggacttggtc atgtttcgct ccggtacagg tatggggggt
    19261 cgcccaacgg tcaatacccg tgcatcgatt atgcgcttcg tatcccttgg ccagcaaggg
    19321 cgtacctccg agtggagctg acgggcaggc gcgatttctt ggctgcagtt cggttgcgca
    19381 tcctgttagc gctgacggta gagcgcaatg ggaatccacc attcttcaag cacctgcatt
    19441 gcaaaatcgc tgttcgcaga atcctcgctg aaagctacct cctccaggag tgcgacgatc
    19501 acggcttcct gttccgctgt gagagacgct agtcgtggag gctcccgatc cggtggtcgc
    19561 agactatgga gcaatccttc aaccgccatt tctggatcgt ccatgtgtcg gagcgcatag
    19621 ttgatcaggt ggggcaggta gtgccgccac gaagcagggt cgagatgggc gagaccccag
    19681 aaagtgtatg tttcgaggta cgtatccgtc ggcttgtcga gaaccgggtc aggtggcgga
    19741 gcgaagtcgt agctgtccac ggcgtagcca ccgcgcagcg tcatccgggg ggcggccaaa
    19801 gcgtcggaat gaaacacttc acgggctcgg cggctgatct cggatcgcat ccaaatctcg
    19861 ttgcatgatc tgtctcactc caatgaaccg acattgtctt ctgcccaccc gagaaatacc
    19921 tcaaggatgc gctccagcat tgtttcgtca ccggcaccat gccaagtgcc attctcgatg
    19981 taacaactca gccaatgtga gccaagagca aaacgcaggt catcaacacc ttccgcgatt
    20041 tcggcgaact gctgcgtctg taaagccgtg cccgtgagat tgattgttac ccaccatccc
    20101 ggattatcgc aagactgaat gttcacgccg gacgaatgct cccattcgcc attgcattgt
    20161 gccaagtacc atgtctgcag acgcgcgagt gtattcatgg ctgccaacaa tagagttgag
    20221 tttcaccccc actgtgcggg gaattcaatg acggccggct tcgggaccgg caccgcccgt
    20281 acaagcgggg caagctcgcg agcatcgcga gcgatggtgc tcgaacgttc aagttcggcc
    20341 gccacacttt acgcggcaag ctcttgcaaa acctcgggat ggcgttcggc gacccggaga
    20401 agggtttttg ccgcgcctga tggttcgcgg cgaccttgtt cccagtcctg gagtgtgcgc
    20461 acagaaacgc cgagcagcgc tgcgaaggcg gtttgagaaa gcccggaca
//
LOCUS       DAORPM010000010        19832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_471_length_19832_cov_16.018506, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000010 DAORPM010000000
VERSION     DAORPM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 19832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19832
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_471_length_19832_cov_16.018506"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLD53_01020"
     CDS             complement(<1..372)
                     /locus_tag="PLD53_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59514.1"
                     /translation="MKFGISTHAPTWGNLKHRLISMVLVLFAMAAVPAMATTGHVSEA
                     DLVLPDLSDLSLVTFFSGTSGWTLLSYGLLVCIGGLIFGAVIYGQIYRLPVHRSMAEV
                     SELIYETCKTYMITQGKFLLIL"
     gene            complement(878..1903)
                     /locus_tag="PLD53_01025"
     CDS             complement(878..1903)
                     /locus_tag="PLD53_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59515.1"
                     /translation="MGILDWFKSRPSFFEPDRLSDDTTTRAIEKAVTLTNPRMKLIHS
                     YRERLAPAVDISVQYLREMVRAVPPALSVSSVNWSTDPVLRAFFVAAQDIPSTMGRSK
                     NLRTLFNKYPEIDDACFILVMKFNEQQVFGTSMQGNVVQRDVAQTTVGFSDHEARICG
                     LDDAEVRRLLGVQAYEYLLAQALSEIGDERSERRELEDNRALIRARLRLLQQQGPGLG
                     SVFGRVAASSDDEFRLETQLTENERQIEAICSQQSVLDAELESLGAVLRHPERYIRIE
                     QKQLRLNSMNVLVDDASNAVASEIVFSLAQLTGVREIQRAFVMARFARSGLPEARMNL
                     DDIARYL"
     gene            complement(2069..3376)
                     /locus_tag="PLD53_01030"
     CDS             complement(2069..3376)
                     /locus_tag="PLD53_01030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRC59516.1"
                     /translation="MAKNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTL
                     VVGNNVYKLNLVPSGIVHPGIKCYIGNGVVLDIHHLVSEINELEAGGIEVRSRLRISP
                     GCPLILGYHSALDRAREAAKSLDSRIGTTGKGIGPTYEDKVARRALRVYDLSFPERFA
                     EKLKENLDYHNFVLTRYLGAEAVDFNTVLDLAMANAEQIKPMVVDVSAELNAANKAGQ
                     NLLFEGAQGALLDIDHGTYPFVTSSNCVAGQASAGAGVGPGMLHYVLGITKAYCTRVG
                     GGPFPSELDIETADTPGYQMSHKGCEFGTVTGRKRRCGWFDVPALKRSSQINGVSGLC
                     ITKLDVLDGLPELKICTGYTLNGKLVDLLPMGSDEVAGCQPVLESMSGWVESTAGATS
                     LDELPAAARAYIARIEALCETRVDIISTGPERNETILRRHPFA"
     gene            complement(3385..4539)
                     /locus_tag="PLD53_01035"
     CDS             complement(3385..4539)
                     /locus_tag="PLD53_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRC59517.1"
                     /translation="MNWLLPEYIADVLPHEAAHIELLRRAVLDLFRVRGYELVMPPLL
                     EHLDSLLTGSGSDLRLRTFKLVDQLSGRTLGLRADMTPQVARIDAHLLNRQGVTRLCY
                     CGSVLHTLPALQASSREPIQLGAELFGYAGIEADLEAIRLMAVVLGTAGIPASRIDIG
                     HVGVFRALAGAAGLADDVEAKILQLLQVKDIPSLHDACEKVAEPYRSAFLRLPELYGD
                     GNILETASRELPALPEIASALEAMRHLQTSLPELPLSFDLADLRGYHYHNGVVFAAYY
                     PGFPSAIALGGRYDGVGKDFGRTRPATGFSMDLREVARLAVSGKAGGAIMAPHSATDS
                     ALASRIEALREQGEVVVELLPGESCCEGPLCDRQLVENDGQWTVRETIGD"
     gene            complement(4536..4721)
                     /locus_tag="PLD53_01040"
     CDS             complement(4536..4721)
                     /locus_tag="PLD53_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HRC59518.1"
                     /translation="MTPSLLVAFALMLVLEGLVPFLAPGIWRETFRRLIQLSDGQIRF
                     VGLTSMLIGIMLLMVFR"
     gene            complement(4718..5608)
                     /gene="hflC"
                     /locus_tag="PLD53_01045"
     CDS             complement(4718..5608)
                     /gene="hflC"
                     /locus_tag="PLD53_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRC59519.1"
                     /translation="MKSGLNFIAAIVAAILVVLGATIFTVDQRKFAIVFQFGEVREVI
                     SEPGLYFKWPMIQNVRYFDKRILTLDNTEPERFITSEKKNVLVDSFVKWKIIDPQLYY
                     VSVAGDEIRAKTRLSQTVNAGLREEFGKRTVHDVVSGERDLIMEQMRDKADQDARKIG
                     VQIIDVRVKRVDFPPEVSESVYRRMEAERKRVANALRSEGFAEAEKLRADADRQREVI
                     VAEAYRDAQKMKGEGDAKATATYAQAFGQNPEFYAFYRSLEAYRNSFNGKNDVIVVEP
                     NSDFFKYMKSIGRGGEKTGK"
     gene            complement(5608..6870)
                     /gene="hflK"
                     /locus_tag="PLD53_01050"
     CDS             complement(5608..6870)
                     /gene="hflK"
                     /locus_tag="PLD53_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRC59520.1"
                     /translation="MSLNDPQWGNRGNDNGGGKRPNQGPPDLEELWRDFNRRLSGVFN
                     KKRGGGNGGDGNRPPVEFNPKFLGGGLGLLVVLVLVVWMASGFYIVDASQRGIVLQFG
                     KYKQSTESGLNWRFPYPIQSHELVNISGVRTLEVGYRNDQKNKVLREALMLTDDENII
                     NIQFAVQYILKDPVEYIFNNRSPDDAVMQVAESAIREIVGKSRMDFVLYEGREQIAVK
                     ASTLMQEILDRYKTGILISKVTMQNAQPPEQVQAAFDDAVKAGQDLERQRNEGQAYAN
                     DVIPKSRGTAARLLQEADGYKQRLIATAEGDASRFKQINTEYAKAPEVTRSRMYIETM
                     QQIYANTSKLLVDAKGQGNLLYLPLDKLMQASGALVASPESVGSSSEMPAQVRSGPGA
                     SNFSPSQMGDRVDVRSRDALRSRDREAR"
     gene            complement(6891..8063)
                     /gene="hflX"
                     /locus_tag="PLD53_01055"
     CDS             complement(6891..8063)
                     /gene="hflX"
                     /locus_tag="PLD53_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRC59521.1"
                     /translation="MIERLATGERAVIVQLDFGCGAMTEELEEVRLLAESAGALVCAE
                     VYGRRHSPDAATYAGKGKVQEVAAEVTAHEADLVIFNHELSPAQERNIERVLQCRVID
                     RTSLILDIFAQRAQSSEGKLQVELAQLDHLATRLIRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLGKRVKVLKERLIRLERQRSVQRRARGRGDVLNVSLVGYTNAGKSTLFNALT
                     HAGAYAANQLFATLDTTSRKLWLGEAGNIVISDTVGFIRNLPHELVAAFHATLEATSQ
                     ADLLLHVVDSASPARDDQVAEVDKVLTEIGANEVRQLVVLNKQDLTGLSPAVERDEYG
                     RITRVRLSAKCGSGLPLLREALTEIALEKLRNPRAPPTDSDTSFNPNITLDSGFSL"
     gene            complement(8178..8417)
                     /gene="hfq"
                     /locus_tag="PLD53_01060"
     CDS             complement(8178..8417)
                     /gene="hfq"
                     /locus_tag="PLD53_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRC59522.1"
                     /translation="MSNKGQLLQDPFLNALRREHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLKNTVTQMVYKHAISTVVPSRPVSIPQDGSSEA"
     gene            complement(8502..9947)
                     /gene="der"
                     /locus_tag="PLD53_01065"
     CDS             complement(8502..9947)
                     /gene="der"
                     /locus_tag="PLD53_01065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRC59523.1"
                     /translation="MKPIIVLVGRPNVGKSTLFNRLTRTRDALVADIPGLTRDRHYGH
                     GKLGGKPCLVVDTGGFEPLAKDGIMHEMARQTEQAIAEADAVIFVVDGRTGITALDKE
                     IANKLRRAVRPVFVAVNKVEGMNQGVVIADFHELGLGQPFAISATHGEGVHGLIELAL
                     EPYPVPVESESENDDLKVAIVGRPNVGKSTMINALLGEERVIAFDQPGTTRDAIYVDF
                     ERAGKHYTLIDTAGLRRKGNVFETAEKFSVIKTLQSIEDSHVVILVLDAQQDISDQDA
                     HIAGFVVESGRALVVAVNKWDGLDSYARDQIKAALEGKLKFLDFADFLYVSALKGQGL
                     ERVFRSVNAAYKAATADLSTPQLTRTLIDAVARQSPPRNGIFRPKLRYAHQGGRNPPV
                     IVIHGNALDKVPESYRRYLEHVFREVFKLQGTPLRIQFNVSKNPFADRKPPEQNRRKP
                     KPKVPISGKPKPKAASRAKAGPQAKAGSTPA"
     gene            complement(9944..11095)
                     /gene="bamB"
                     /locus_tag="PLD53_01070"
     CDS             complement(9944..11095)
                     /gene="bamB"
                     /locus_tag="PLD53_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HRC59524.1"
                     /translation="MKRCFAVVFVATMLAFLLGGCSTLDSLNPFASNSPKMAELKPIQ
                     PTADARVVWRESVGKSDLYAFAPAVVGTSVYAASKGGDLMRIDDGKLAWKINAGLPLS
                     GGVGANENLVVVGSPKGDLLAFSSADGKLLWQAKATSEILAPPALGEGIVVVRSGDNR
                     LAAYDVLDGNRKWIFQRPTPALSLRVTAGPVIAGKYVFAGFPGGKLIAVSTANGAPLW
                     DGTVALPKGSTELDRVADITSAPVIAGRAICSVAFQGRVACFDLGNGNLVWAREMSSA
                     VGLAIDSGNLYVTDDKGSVHALDMASGASIWKQDKLSLRRVTAPLPRRGMVVVADAQG
                     VVHFLSREDGSFVARMATDGTPVIAPLQALGNDFVVQTSGGGVYAIEAR"
     gene            complement(11092..11778)
                     /locus_tag="PLD53_01075"
     CDS             complement(11092..11778)
                     /locus_tag="PLD53_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC59525.1"
                     /translation="MATYDLEEQEQLAEIKVWWKQYGNLVINVFTAVMLAVSAWMGWN
                     WYQRSQSAQASIVFNVLQKAVQDKDSQRTKAASGELLDKFGKSTYASLGALTAAKEMV
                     DAGDVKTAKLQLQWVVEHAEGELRDLARLRLATVLLDEKEYDQALKQLDGNVSPGFAA
                     LFADNRGDVLSAQGKKAEALEAYKVALARLDNSDAKGAKSAQDRQSPSNTVYRELLQQ
                     KLDSLGGGKQ"
     gene            complement(11778..13073)
                     /gene="hisS"
                     /locus_tag="PLD53_01080"
     CDS             complement(11778..13073)
                     /gene="hisS"
                     /locus_tag="PLD53_01080"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRC59526.1"
                     /translation="MSQHIQSVRGMNDILPGEAEFWDVFEETVRSWLKSYGYRPIRLP
                     IVEPTPLFKRAIGEVTDIVEKEMYSFVDSLNGEPLTLRPEGTAGCVRALIQHKLAAQQ
                     PQRLYYQGQMFRHERPQKGRYRQFNQVGVESFGFSGPDIDAEQILMGARLWADLGLDG
                     IKLQLNTLGQPEERANHRAELIAYFEKYADVLDEDARRRLYTNPLRILDSKNPALQEL
                     IVMAPLLTNHLGSDSMAHFEGVQQVLRDAGQPFEINRRLVRGLDYYNLTVFEWVTDRL
                     GAQGTVCAGGRYDGLVEQLGGKSTPACGFAMGVERLVALIRESGGEPALEGIDVYLVH
                     QGEAASRLAPRVAEGLRDQGINVLFHCGDGGFKSQMKKADASGAAFAVIIGDDEANAG
                     QVMLKPLRANGEVQNEQRRVGVDSIADEIMNSFLDWEEA"
     gene            complement(13070..14344)
                     /gene="ispG"
                     /locus_tag="PLD53_01085"
                     /gene_synonym="gcpE"
     CDS             complement(13070..14344)
                     /gene="ispG"
                     /locus_tag="PLD53_01085"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRC59527.1"
                     /translation="MSDASIYGEHSVSKRCAMRRQTRAVRIGHVTVGGESPIVVQSMT
                     NTDTVDVVRTAVQCAELARAGSELVRITVNTSEAAAAVAKIREHLDTMGVDVPLIGDF
                     HYNGHRLLADHPECAQALAKYRINPGNVGHGRKRDEQFAQMIEIACKFDKPVRIGVNW
                     GSLDQTLLARIMDENAKRNEPKDAIEVMREAMVTSALESSAMAESLGLPADRIILSCK
                     VSGVQDLIAIYRELSGRSDYPLHLGLTEAGMGSKGIVASTAAMSVLLQEGIGDTIRVS
                     LTPEPGGKRTQEVVVAQEMLQTMGLRAFTPMVAACPGCGRTTSTFFQELAHSIQEHVR
                     DNMPQWRVEYDGVENMTLAVMGCVVNGPGESKHANIGISLPGTGEAPVAPVFEDGAKT
                     VTLRGDKIAEEFTAIVDRYVARKYQRKFAVKA"
     gene            complement(14346..15293)
                     /locus_tag="PLD53_01090"
     CDS             complement(14346..15293)
                     /locus_tag="PLD53_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRC59528.1"
                     /translation="MTSVNVPSSGSDNEANVEPDMTQTPGSFEATQDSVVLSVGQQLR
                     AAREARGLTADDVAKAIKLSFRQVKALEADDWPNLPGKTIIRGFVRNYARLLNLNSDQ
                     LMTELDRLHMPQAPELDIPTGPTVNLQNDGKTDRRDFFTVFAGLFVLLLAVLSYFLVS
                     QDLWQSALSALKSATQSSEVVVEKVGAPAASGTTASVAVPVSLTPLTPPAATVSSAGA
                     TASPPADPAPVSAVALVPANGLKFSFDQPSWVEVRDRSGEVIFSQLCQAGSQRDIEGQ
                     PPFSLVVGNSSHVTLLYNGKAVDFSKHRSKDDVARLTLE"
     gene            complement(15290..16096)
                     /gene="pilW"
                     /locus_tag="PLD53_01095"
     CDS             complement(15290..16096)
                     /gene="pilW"
                     /locus_tag="PLD53_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HRC59529.1"
                     /translation="MKVLHRIFASCCFRAVALTLVAVSSALAQQSVAPPVEPLSSMTP
                     KDAHTRAKLHTELGSLYFRNGDLIVALEELTIAISIDANYAQAYSTRGLVLYQIKEFA
                     SAEKDFQRALDLAPKDPQISNNYGWFLCNTGKEKESIEYFRRAINDPLYQTPALAHLN
                     AGTCYARINELDLAEDYIRKTLRYMPDNPQALLQLANISYKRGNYDAAKEHLKKVVRL
                     SNPNAEALWLFLRVERRLGNSSDEISLAAQLRRKYPDSPEYQEFLKGNFE"
     gene            complement(16093..17187)
                     /gene="rlmN"
                     /locus_tag="PLD53_01100"
     CDS             complement(16093..17187)
                     /gene="rlmN"
                     /locus_tag="PLD53_01100"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRC59530.1"
                     /translation="MSVNLLDYDAAGLAALFAEYGEKPFRARQVLRWIHRFGRSDFDA
                     MSDIAKSLREKLKAVASVIPPTIVSDKLSDDGTRKFLFDVGGGNAVETVFIPEDDRGT
                     LCISTQAGCALDCSFCSTGKQGFNRNLTVAEIIGQLWQAERALETGLKDERIVSNVVL
                     MGMGEPLANFESTVTALRLMLDDNAYGLSRRRVTVSTSGLVPAMDRLGEECPVALAVS
                     LHAPNDRLRNELVPINQKYPLGELMAACNRYLQYAPRDFVTFEYVMLDGVNDADSQAY
                     ELLDLTRKVSCKINLIPFNPFPGSPYRRSPAYRIRRFAEVLMNAGVVTTTRKTRGDDI
                     DAACGQLAGQVLDRTRRSARRIIDLHEVTR"
     gene            complement(17207..17635)
                     /gene="ndk"
                     /locus_tag="PLD53_01105"
     CDS             complement(17207..17635)
                     /gene="ndk"
                     /locus_tag="PLD53_01105"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRC59531.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGKIYSRFETNGLKIIASKMAWLSP
                     QEAGEFYAVHKERPFFKDLVSFMISGPVMIQVLEGESAIAKNRDLMGATDPKKADPGT
                     IRADFAESIDANAVHGSDGEETAKNEIAFFFPAMSVYSGR"
     gene            complement(17818..19101)
                     /locus_tag="PLD53_01110"
     CDS             complement(17818..19101)
                     /locus_tag="PLD53_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRC59532.1"
                     /translation="MSAVIIFVLLTFLMLSGMPVSISLGLTVLTFLFTFTQTPLDSVA
                     LKLFTGIEKFEIMAIPFFILAGNFLTHGGVAKRMIFFATSMVGHLRGGLGLSAVFACA
                     LFAAVSGSSPATVVAIGSILLPAMVRAGYPKKFGVGVIASSGGLGILIPPSIVMVMYA
                     VSTNSSIGALFMAGVIPGLMLALFLGFTTWYEAKKGNYPREQPRSWAERWDAFRKAIW
                     GLLLIVVVMGGIYSGLFTPTEAAAMSAVYAFFIAVFVYKDIGMKDVPKVLLNSANMSA
                     MLLYIITNAVLFSFILTNEQIPQGIAEWLTHSGVGMVGFLIAVNILLLVMGSFMEPSS
                     IILITAPILFPIAMKLGIDPVHFGVMITVNMEIGMITPPVGLNLYVASGIAKMGLTDC
                     SKAVWPWLLTMLVYLMIITYVPAITTWLPTMLGMM"
     gene            complement(19098..19823)
                     /locus_tag="PLD53_01115"
     CDS             complement(19098..19823)
                     /locus_tag="PLD53_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRC59533.1"
                     /translation="MMKFLDHLEEWLITFLIGGATLIIFTAVMHRYAAGVPIPVVQDW
                     LLSLNLSWAQELCIIMFVWMAKFGAAYGVRTGIHVGVDVLINSLSGRTRAKFIVFGMS
                     AGAFFTGIVGTLGAHFVWENGAHYAIFNALGLATGDLYPGPTTPDLEWQTWIVYSAIP
                     LGSYLMCFRFLQVTVGFIRTGELPHHDHGHVDGVDEDEVPVDINPYNMDDDLHMHDLK
                     YKKRHPEQQVGDGGTAADKGEPK"
BASE COUNT         4478 a         5875 c         5314 g         4165 t
ORIGIN      
        1 caggatcagc aggaacttgc cctgggtgat catgtaggtc ttgcaggttt cgtagatcag
       61 ttcgctgacc tcggccatcg agcgatgtac cggaagacgg taaatctgac cgtaaatcac
      121 cgcaccgaag atgaggcctc cgatgcaaac cagaagtccg taggaaagca aagtccagcc
      181 tgatgtcccg ctgaagaacg tcaccagcga aagatcgctt aggtcgggca aaacaagatc
      241 ggcttcggaa acatggcccg tggtcgccat agccgggacc gcggccatcg cgaatagaac
      301 gagcaccatc gaaatcagac ggtgcttcag gttgccccat gtcggtgcat gggtggaaat
      361 accgaacttc atcaaaaacc cccaatctac aagttgtcac caccaagaat tgatcccgcc
      421 catcggccca aataatttgt gcaagtttct attattcagt tcagcaaagc tgattacaca
      481 gaggacaaag ttacctttgg caatacctca attcgatcag tcacaagcgg cccggcgcca
      541 gcagccctga ttgctgccgc attttatcag tgaactgtta gtggcgctcg tttgcaaccg
      601 aaccccccgt tccccatcac tcggcgaaaa cgcggaccct gctcgtgccg gataatgtca
      661 tcgctggtga cgaatagccg ggttggccat ggcactttcc tctgtgttga cttagcaagg
      721 aaagtgccaa tgtgtagcct actgaaatat aacgatatgg atttccgcca ctgccggaat
      781 gatcgttcga tgtagccaat acgacaatca gcattgcggt tgcctaaagg gttgccgatt
      841 cctgagaaca ttcccttgtg caggacgtca accaacttca aagatagcgc gcaatgtcat
      901 caagattcat cctggcctct ggcaatccgg aacgtgcaaa acgggccatg acgaacgctc
      961 gctgaatttc ccgcacgccc gtcaattggg ccagcgaaaa tacaatttcg gaagcgacag
     1021 cattgctcgc atcatcgacc agaacgttca tcgagtttag ccgaagctgc ttttgctcga
     1081 tgcgtatgta gcgctctgga tgccgaagta ccgcgcctag cgattccagt tcggcatcaa
     1141 gcactgactg ctgactgcaa atcgcctcaa tctggcgctc gttttccgta agctgtgttt
     1201 ccagcctgaa ctcgtcgtct gaactcgctg ccaccctgcc gaaaaccgac ccaaggcccg
     1261 ggccctgttg ctgaagcaag cgcagtctgg cacggatcaa cgcgcggttg tcttcaagct
     1321 cacggcgctc ggagcgctca tctccgattt ctgacaaggc ctgtgccagc aaatactcat
     1381 acgcctgcac ccctaggaga cgccgaacct ctgcatcgtc aagcccacaa atgcgtgctt
     1441 cgtggtcgga aaaaccgacg gtggtttggg caacatcacg ctgtacgaca tttccttgca
     1501 tcgacgtacc gaagacttgc tgctcattga acttcatcac gagaatgaaa caggcgtcgt
     1561 caatctccgg gtacttgttg aacagggtac gcaaattttt tgatcggccc atcgtggaag
     1621 gaatatcctg tgcagcaaca aagaaagctc tcaacaccgg gtcggtggac cagttcacgg
     1681 acgaaaccga tagggccggc ggaactgcgc gcaccatctc gcgcagatat tgaacactga
     1741 tatccaccgc tggtgccaga cgctcccgat acgaatggat gagtttcatt cgtggattgg
     1801 tcagtgttac ggccttctcg attgcccgcg tcgtcgtatc atcggacaga cgatcgggct
     1861 caaaaaacga tggccgactc ttgaaccagt cgagaattcc catggcctac ttctcctcgc
     1921 gatgtctgca catcagactc gagcgcaatg acttgactac gagctgttcg tcaatggata
     1981 ggttcagggg aaatcttgcg tgttaactaa aaagccagct ccagaccggc tttaccgacc
     2041 gtcgatgaga ttgatggcgt ctgtcagatc acgcgaaagg atgtcggcgc agaatcgttt
     2101 cattgcgctc gggtccggtc gatatgatat cgacgcgcgt ttcacacaat gcttcaatac
     2161 gggcgatata agcgcgcgcc gccgctggta actcatccag cgaggttgct ccggcagtgg
     2221 actcgaccca ccccgacatg ctttcgagca cgggctggca accagccact tcgtcagacc
     2281 ccattggcag caggtcgacg agttttccgt tgagcgtgta tccggtgcag attttcagct
     2341 ccggcaaacc atccagcaca tcaagtttgg taatgcacaa accgctcacg ccattgatct
     2401 ggctggaacg tttaagcgcg ggaacatcga accatccgca acgacgcttg cgcccggtaa
     2461 cggtgccaaa ctcacaccct ttatgcgaca tctgatatcc gggagtatcc gccgtctcga
     2521 tatcaagctc gctcggaaag ggccctcctc cgacccgcgt acagtaggcc ttggtgatac
     2581 ccaaaacgta gtggagcatg cccggcccaa cgcccgcccc ggccgatgcc tgtccggcaa
     2641 cgcagttcga cgacgtaaca aagggataag tcccatggtc gatgtcaagc aaggcgcctt
     2701 gtgcgccttc aaacagaaga ttctggccgg ccttgtttgc cgcgttcaat tcggctgaaa
     2761 cgtcaacaac catcggtttg atctgttcgg cattggccat cgccagatcc aggacagtgt
     2821 tgaaatcgac cgcttcggct ccaagatagc gagtcagcac gaaattgtga tagtcgaggt
     2881 tctccttgag tttctctgca aaacgttcag gaaaggacaa atcgtaaaca cgcaaggccc
     2941 ggcgcgccac tttgtcctcg taggtcggac caatgccttt gcccgtggtg ccgatcctgg
     3001 agtccagact cttggccgcc tcgcgcgcac gatcaagcgc cgagtgatat ccgagaataa
     3061 gcgggcaacc cgggctaatt ctgaggcgcg agcggacttc aatccctccc gcctccagtt
     3121 cgttgatctc ggatacgagg tgatggatat ccagaaccac accgttaccg atatagcatt
     3181 tgattcccgg atgaacaatg ccagagggaa ccagattgag cttataaaca ttgtttccta
     3241 caaccagcgt atggccggca ttgtgaccgc cctgaaaacg cactaccccc tgcgcatggt
     3301 cggtcaacca gtcgacgatc ttgcccttgc cctcatcacc ccactgtgtg cccactacta
     3361 caacgttctt agccattttc ccagtcaatc cccgatagtt tcccgaaccg tccattgtcc
     3421 gtcgttttcg accaattgcc gatcgcacag aggcccctcg caacacgact cacccggcaa
     3481 cagttctacg acgacttcac cctgttcgcg caacgcctcg atccgagacg ccaaagcgga
     3541 atcagtcgcg gaatgaggcg ccatgatggc ccctcccgcc ttgccactca cagccagccg
     3601 ggcaacttcg cgtagatcca ttgagaaccc ggttgccggc cgggtacgac caaagtcttt
     3661 acctacgcca tcgtaacgcc cacccaaggc aatagcgctg gggaaaccgg ggtaataggc
     3721 ggcaaacaca accccgttgt gatagtgata gccacgcaag tcagcaagat caaaggacag
     3781 cggcaactcc ggcaacgagg tctgcaaatg tcgcatcgct tccagcgccg aagcaatttc
     3841 gggtagcgcc ggcaattcac gggaggcggt ctccagaata ttgccgtcgc catacagttc
     3901 aggtaggcgc aggaaggcgg agcgataggg ctcggccact ttctcgcaag catcgtgcag
     3961 gcttggaatg tccttgacct gcagaagttg cagaatcttc gcctcgacat cgtcggcgag
     4021 gcccgcagcg ccggcaagtg ctcgaaatac cccgacatgc ccgatatcaa tgcgcgaggc
     4081 aggtatgccc gccgttccga gaaccacagc cataagacgt atcgcttcga gatcggcctc
     4141 aataccggca tagccgaata gttccgcccc aagctggatc ggctcccggc ttgatgcttg
     4201 caaagctggc agcgtatgta acacgctacc gcaatagcag aggcgagtga caccctggcg
     4261 attgagcaag tgcgcgtcga tccgagcaac ctggggcgtc atgtcggcgc gcaggccgag
     4321 cgtccgacct gacaactggt cgaccaattt gaaggtgcgc agcctgaggt cggaccccga
     4381 tccggtcaac agggaatcca ggtgctcgag cagcggaggc atgacaagtt cgtagccgcg
     4441 cacccggaag aggtcgagca cggcccgacg gagcagttct atgtgggccg cttcatgtgg
     4501 aagcacatcg gcaatgtact cgggtagcag ccaattcatc tgaaaaccat caacaacatt
     4561 attccaatca gcatcgaagt caggccaacg aaacgaatct gcccgtctga gagctggatc
     4621 agacggcgaa acgtctcgcg ccagatcccg ggggcgagaa acggcaccaa accctcaagc
     4681 accagcatca gcgcgaaggc gaccagcaaa ctgggggtca tttacctgtc ttttcgccgc
     4741 ctcgcccgat actcttcatg tacttgaaga aatcagaatt cggctccacg acaatgacat
     4801 catttttccc attgaaactg ttgcgatagg cttcaaggct gcgatagaaa gcatagaact
     4861 ccggattctg tccgaaggcc tgcgcatacg tggccgtggc cttggcatcg ccctcacctt
     4921 tcattttctg cgcatcgcga taggcttcgg cgacgatgac ttcacgttgc cggtccgcgt
     4981 cagcgcggag tttctcggct tcggcaaaac cctcggagcg gagcgcgtta gccacgcgct
     5041 tgcgttccgc ttccatacgg cggtagaccg attcactaac ttccggggga aaatcaactc
     5101 gcttcacgcg aacatcgata atctgaacac caatcttgcg cgcatcctga tccgccttgt
     5161 cgcgcatctg ctccatgatc aaatcgcgtt cgcctgaaac cacatcatga acagtacgct
     5221 tgccgaattc ctcccgcaaa ccggcgttta cagtttgcga caaacgtgtc ttggctcgaa
     5281 tctcatcacc agcgaccgac acatagtaaa gttggggatc gattatcttc catttgacaa
     5341 aggaatcgac cagcacgttc ttcttttcgg aggtaatgaa acgttccggt tctgtgttat
     5401 caagcgtaag aatccgttta tcgaagtaac gcacgttctg gatcatcggc cacttgaagt
     5461 acaggccggg ctcggatatg acttcgcgga cctcgccaaa ctggaaaaca atggcgaact
     5521 tccgctgatc gactgtaaag atcgtcgcac ccagaacaac caatatcgcc gcgacgatgg
     5581 cagcaatgaa atttagaccg gatttcatta tcgtgcctcc cgatcccttg agcgcagggc
     5641 atcgcgcgag cggacatcaa ccctgtcccc catctgtgac ggtgaaaagt ttgaagctcc
     5701 gggcccggag cgcacctgcg ccggcatctc cgaactgctg ccgacactct ctggagacgc
     5761 tacaagcgct ccagacgctt gcatcagctt gtcgagtggc aagtagagca gattgccctg
     5821 gcccttggca tcgaccagca gtttgctggt gttggcgtaa atctgctgca tggtttcaat
     5881 atacattcga ctgcgcgtca cttcaggcgc tttggcatac tcggtattga tctgcttgaa
     5941 acggctggca tcgccctcgg ccgtagcgat taggcgctgc ttgtaaccgt ctgcttcctg
     6001 caacaggcga gccgccgtgc ctctggattt cggaatgacg tcattggcat acgcttggcc
     6061 ttcattcctc tgccgctcaa gatcctgacc ggccttcaca gcatcatcaa acgctgcctg
     6121 aacctgttcg ggcggttgag cattctgcat tgtcactttc gaaatcagga ttccggtctt
     6181 gtagcgatcg agaatctcct gcatgagggt ggaagctttt accgcgatct gctcgcgccc
     6241 ctcgtaaagc acgaaatcca tcctgctctt gccgacgatt tcgcgaatcg ccgattcggc
     6301 cacctgcatt acagcatcgt cgggagaacg gttgttgaag atgtattcaa caggatcctt
     6361 gagaatatac tgaacggcaa actggatgtt gatgatgttc tcgtcatccg tcagcatcag
     6421 ggcttccctg agaaccttgt ttttctggtc gttgcgatag cccacttcaa gcgtacgcac
     6481 accggatata ttgaccagtt catgcgattg gatcgggtaa ggaaaacgcc agttcaaacc
     6541 ggactccgta ctttgcttgt acttgccgaa ctgcaggacg ataccgcgct gcgaagcgtc
     6601 cacgatataa aacccactgg ccatccagac cacaagtaca agaacaacga gcagaccgag
     6661 gccaccaccg agaaacttgg gattgaactc gactggcggt cgattgccat cgccaccatt
     6721 gccgccgccg cgcttcttgt tgaacacgcc ggacaggcgg cgattgaaat cacgccacaa
     6781 ttcttcgagg tccggtggcc cctgattcgg tcgtttgccg ccgccgttat cgttaccgcg
     6841 attgccccac tgcggatcat taagcgacat gagtgttcca atgcatgaaa tcatagggag
     6901 aaacctgagt ccaacgtaat atttgggttg aacgaagtat cactgtccgt cggcggagcg
     6961 cgaggattgc gaagcttctc aagtgcaatc tcggtcagcg cttcgcgtaa caggggcaga
     7021 ccactgccac acttcgcact gagccgaacg cgcgtaattc taccatactc gtcccgttcg
     7081 acggctggag acagccccgt gagatcctgc ttgtttagga cgaccagttg ccgaacctca
     7141 tttgcgccga tttcagtcag gaccttgtcg acttcggcaa cctgatcgtc tctagcagga
     7201 cttgccgaat cgacaacatg caacaggaga tcggcttggg atgtcgcctc cagcgtcgca
     7261 tgaaaagccg ccaccaactc gtgcggcaaa ttgcggataa aaccgaccgt atcggaaata
     7321 acaatattcc cggcttcgcc aagccagagt ttacgggatg tcgtatcaag tgtggcaaac
     7381 agctggtttg cagcataggc accggcatgg gtcagcgcgt tgaaaagggt tgatttgccg
     7441 gcgttggtat agccgacaag ggaaacattc agcacgtcgc cgcgtccgcg ggccctgcgt
     7501 tgtacactac gttggcgttc gagtctgatc agccgctcct taagcacctt gacccgcttg
     7561 ccaagcaggc ggcggtcagt ttcgagctgg gtctcgccgg ggccgcgcag tccgattcca
     7621 cccttttgac gctccaaatg ggtccagccg cgaatcagcc tggtcgccag atgatcaagt
     7681 tgcgccagtt caacctgcag cttgccttcg gagctctgtg ctcgttgtgc aaagatatcg
     7741 agaatcaggc tggtccggtc aattacacgg cactgcagta cgcgctcgat attgcgttcc
     7801 tgggcggggg ataactcatg gttgaaaatg acgagatcgg cttcgtgcgc agtgacttcg
     7861 gccgccactt cctgcacctt gcctttgccg gcatacgtcg ccgcatcagg actatgtcgc
     7921 cgtccgtata cctctgcaca aaccaaagcg ccggccgact cagcaagcag acgcacctct
     7981 tccagttctt cagtcatggc accgcaaccg aaatcgagct ggacgatgac agcacgttcg
     8041 cccgtggcaa gacgttcaat catcaaagcg cttacgtatg acgaagtgag tgcaaaagtc
     8101 gggccgaagc tcgctatcgg gactgctcga gtaggtggtc ctatagggca gactgaagga
     8161 actcggtgga aggcagatca agcctcgcta cttccatcct gtgggatgct gaccgggcgt
     8221 gaaggcacca cggtggatat cgcgtgtttg tagaccattt gggtgacggt gttcttgagc
     8281 agaacgacgt actgatcaaa cgactcgacc tgcccttgca acttgatgcc gttgaccaga
     8341 tagatcgaga ctggcacatg ttcacggcgc agcgcattga ggaaggggtc ttgtaacagt
     8401 tgccctttat tactcatggt taggactcca tgtttcgtgg tcggtgtatg gcagctcagc
     8461 ataattgatt tttaacaggt ttgccatacc aacccgaata ttcaggcagg agtcgaacct
     8521 gccttcgctt gcggtcccgc ctttgctctt gaagcggctt taggcttcgg cttccccgat
     8581 atcgggactt ttggtttcgg ttttcgccga ttttgttccg gcggtttgcg atcggcaaag
     8641 gggttcttcg agacattgaa ctggatgcgc aaaggcgttc cctgaagctt gaatacctct
     8701 cggaacacat gctccagata acgcctgtag gattccggca ccttgtcgag agcatttcca
     8761 tgaatcacga tgacaggcgg attacgtccg ccctggtgcg catagcgcag cttggggcgg
     8821 aaaatcccgt tgcgcggcgg cgactgcctg gccaccgcgt caatcagcgt tcgcgtcaat
     8881 tgcggtgtcg acaaatctgc cgtcgccgcc ttgtacgcgg cgttaaccga acggaacacg
     8941 cgctccaacc cctgtccctt gagcgccgag acatagagaa aatcggcgaa atcaagaaat
     9001 ttcaacttgc cttcaagcgc agctttgatc tgatcgcgcg cataggaatc caggccatcc
     9061 catttattga cggcaaccac cagcgcacga ccggactcga caacgaagcc ggcgatatgt
     9121 gcatcctgat cggaaatgtc ctgctgggca tcaagcacaa ggatcaccac atgggaatcc
     9181 tcgatggatt gcagtgtctt gatcacggag aacttctcgg cagtttcgaa aacgttgccc
     9241 ttgcgccgca aaccggccgt atcgatcagt gtgtagtgct ttcctgcgcg ctcgaaatca
     9301 acgtagatgg cgtcacgcgt tgttcccggc tgatcgaaag cgatcacgcg ctcttcgccg
     9361 agcagggcat tgatcatcgt gctcttgcca acgttcggcc ggccgacaat tgccaccttc
     9421 aagtcatcgt tttcggattc gctttctact ggcacgggat agggttcaag tgccagctcg
     9481 atcaagccgt gtacaccttc accgtgcgtt gctgaaatgg cgaacggctg acccaaccca
     9541 agctcgtgaa aatccgcaat gacgacaccc tgattcatgc cttcgacctt gttcacggcg
     9601 acgaagaccg ggcgcaccgc tcggcggagc ttgttggcaa tctctttgtc gagcgcggtg
     9661 attccggtac gcccgtcgac cacgaagatg accgcatccg cttcggctat ggcctgttcg
     9721 gtttgacgcg ccatttcatg catgatgccg tctttcgcca gcggctcaaa cccccccgta
     9781 tcaacaacca gacagggttt tccgcccagc tttccgtgac catagtgacg atcgcgagtc
     9841 agccctggaa tatcggctac cagagcgtca cgcgttcggg tcagccgatt gaaaagcgtc
     9901 gattttccta cattcgggcg tccgacaaga acgatgattg gtttcatcgg gcctcgatgg
     9961 catagactcc accgccgctg gtctgcacca cgaagtcatt gcccagcgcc tgtaaaggcg
    10021 caatgaccgg agtcccgtcg gtcgccatac gggcgacgaa cgagccgtct tcacgactca
    10081 aaaaatgcac cacaccctga gcgtccgcga cgacgaccat gccgcgacgc ggcaaaggcg
    10141 ctgttacgcg acgcagtgaa agcttgtcct gtttccagat gctggcgccg ctcgccatgt
    10201 caagtgcatg cactgaacct ttatcgtcgg taacgtatag gttgccggaa tctattgcca
    10261 atccaacggc actcgacatc tcccgcgccc agacaagatt cccgttgcca agatcaaagc
    10321 aggcaacgcg cccttgaaac gcaaccgagc agatcgcacg cccggcaatg accggagcgc
    10381 tggtgatatc ggcaacgcgg tcaagttcgg tcgaaccttt tggcagagca accgtgccgt
    10441 cccacaatgg cgcgccgttg gccgtgctga cggcaatcag cttgccacct ggaaaaccgg
    10501 cgaagacata cttgcccgca atgaccggac cagcggtaac gcggagggaa agcgcaggcg
    10561 tcgggcgttg aaatatccat ttccgattgc cgtcgagcac gtcgtaggca gccaagcggt
    10621 tgtctccgct acgcacgacg acgatcccct cgcccagtgc cgggggagca agaatctcgc
    10681 ttgtcgcctt ggcttgccat agaagtttgc catcggcact ggagaatgcc agtagatccc
    10741 ccttcggaga gccgaccaca accaaattct cattggcacc cacgccgccg gagagcggca
    10801 atcctgcgtt gattttccag gccagctttc cgtcatcgat gcgcatcaga tcgccaccct
    10861 tgctggcggc atatacggaa gtgcccacta cggccggcgc aaacgcatac aaatcgcttt
    10921 tcccgacact ttcacgccat acaacacgcg cgtccgcggt cggttggatt ggctttaatt
    10981 cagccatttt cgggctattg ctggcaaagg gattcagcga gtccagcgtc gaacaaccgc
    11041 ctagcaagaa cgccagcatt gttgccacaa aaacgacggc aaaacaccgc ttcattgctt
    11101 tcctccacca agcgaatcaa gcttctgctg caggagttcc cgatagaccg tatttgaagg
    11161 gctttgccgg tcctgcgcgg acttcgctcc cttggcgtcc gaattatcaa gcctggccag
    11221 cgctaccttg taggcttcca atgcttccgc cttcttgccc tgcgcactga gcacgtctcc
    11281 acggttatcg gcaaagagcg cggcaaaacc cggactgacg ttcccgtcga gttgtttcaa
    11341 cgcttggtcg tattcctttt catcgagcag tacggtagcc aggcgcaaac gtgccaggtc
    11401 gcgcaactcg ccctcggcat gttcaacaac ccactgcaac tgcaacttgg ccgttttgac
    11461 gtcgccggcg tcaaccatct ctttggcagc ggtcaacgca ccaagcgacg cgtaggtcga
    11521 ttttccgaat ttatccagca actcgccgct cgctgccttg gttcgttgcg aatccttgtc
    11581 ctgaactgcc ttctgcagga cattgaagac tattgatgcc tgtgccgatt gactgcgctg
    11641 ataccagttc cagcccatcc aggcactaac ggccagcatg acggccgtaa agacattgat
    11701 caccagattg ccgtactgct tccaccacac cttgatttcg gcgagttgtt cctgttcttc
    11761 gaggtcgtaa gtagccatta cgcttcttcc caatccaaaa atgaattcat gatttcgtcg
    11821 gcgatactat caacaccaac gcgtctttgt tcgttctgca cttctccgtt ggcacgcaga
    11881 ggcttgagca taacctgtcc ggcattcgcc tcatcgtcac caatgatcac ggcaaaggcc
    11941 gcgcccgagg cgtcagcttt tttcatctgg gatttgaagc ctccatcacc acagtggaac
    12001 aggacattga taccctgatc acgcaagcct tcggccactc ggggtgcaag ccttgaagct
    12061 gcttcgccct gatgcacgag atagacatcg atcccttcga gcgccggctc gccgcctgat
    12121 tcgcgaatca gcgcgaccag tcgctcaacg cccatggcaa aaccgcaggc aggcgtcgac
    12181 ttgccgccga gctgttcgac caagccatcg tagcgaccac ctgcacacac cgtcccttgc
    12241 gcaccgagac ggtcggtcac ccactcaaag acggtcaggt tatagtaatc gagtccgcgc
    12301 accagtcgcc gattgatttc aaaaggctgc ccggcgtcac gcagaacctg ttgcacgcct
    12361 tcgaaatgcg ccattgaatc ggaacccaga tgattggtca gaagcggtgc cataacaatc
    12421 aattcctgca acgccgggtt cttgctgtcg agaatgcgta gcggattggt gtagaggcga
    12481 cgccgggcat cctcatcaag aacatcggcg tacttctcga aataggcgat cagctcggcg
    12541 cgatgattgg cacgctcttc cggctgaccc agtgtgttca gctgcaattt gatgccgtcc
    12601 agcccgaggt cggcccatag acgggcgccc atcagaattt gctcggcatc gatatcggga
    12661 ccggaaaagc cgaacgactc gacaccgacc tgattgaact ggcgatagcg tcccttctgc
    12721 ggcctttcgt gccggaacat ctgcccctgg taatacaagc gttgcggttg ctgtgccgcc
    12781 agcttgtgct gaatcagcgc acgcacacag cccgcggtgc cttctggacg cagcgtaagc
    12841 ggttcgccat tcaagctatc gacgaaagaa tacatttcct tttcgacgat atcggtcact
    12901 tcaccgatgg cacgcttgaa taggggtgtc ggctcgacaa taggcaaacg gatcggccga
    12961 taaccgtagc tcttcaacca cgagcggacg gtttcctcga agacgtccca gaactcggcc
    13021 tcgcccggca gaatatcgtt cataccgcgc acggactgga tatgttgact catgctttta
    13081 cggcgaactt ccgttggtat ttccgcgcca catagcgatc cacaatggcg gtaaattctt
    13141 cggcgatctt gtcgccgcgc agtgtgaccg tcttggcgcc atcttcgaag accggcgcta
    13201 ccggcgcttc gccggttccc ggcagactga tgccgatatt ggcgtgtttg ctttcgcccg
    13261 gaccattgac cacacagccc atgaccgcca gcgtcatgtt ttcaacacca tcgtactcga
    13321 ctcgccattg cggcatgttg tcgcgcacat gctcctgaat cgagtgcgca agttcctgga
    13381 aaaaggtgct ggttgtccga ccgcaacctg ggcaggccgc caccatcggc gtgaaggcac
    13441 gcaagcccat cgtctgcagc atctcctgtg cgacgacaac ttcctgcgta cgcttgccgc
    13501 ccggctccgg ggtcagcgag actcggatgg tgtcgccaat tccttcctgc aacagcacgg
    13561 acatcgcggc tgtcgaagcg acaatacctt tcgagcccat tcctgcctcg gtcaatccga
    13621 gatggagcgg ataatccgag cgtccggaca actcgcgata gatggcaatc agatcctgaa
    13681 ccccggaaac cttgcacgac agaatgatgc ggtcagccgg tagtcccagg ctttcggcca
    13741 tggctgacga ttcgagcgca gaagtcacca tcgcctcgcg catcacctca atggcatctt
    13801 tcggctcgtt gcgtttggca ttctcgtcca tgatgcgcgc caacagcgtc tgatcgaggc
    13861 tcccccagtt caccccgatg cgtactggtt tgtcaaactt gcaggcgatc tcgatcattt
    13921 gggcaaactg ctcgtcgcgc ttcctgccgt gaccgacgtt gcccgggttg atgcggtact
    13981 tggccaacgc ctgcgcacat tcggggtgat cggccaacaa gcgatggccg ttgtaatgaa
    14041 agtcaccgat caatggcaca tcgactccca tggtgtcgag gtgctcccgg atcttcgcca
    14101 ccgctgccgc ggcttccgac gtattcacgg tgatacgcac cagttcggac ccggctcgtg
    14161 cgagttcagc gcattggacg gccgtcctca cgacatcaac cgtgtcggta ttggtcatcg
    14221 attggacgac gataggcgac tcgccaccga cggtcacatg gccgatgcgg acggctcgcg
    14281 tctggcgacg cattgcgcaa cgcttgctca cagagtgctc gccgtatatt gatgcatcac
    14341 tcatgctatt cgagggtcag gcgagccaca tcatccttgc tgcggtgctt ggaaaaatcc
    14401 accgccttgc cgttgtaaag caaggttaca tgggaggaat tgccaacgac cagtgaaaac
    14461 ggtggctggc cttcaatgtc ccgctgacta ccggcctggc acagctgaga gaatatgacc
    14521 tcgccgctgc gatcacggac ttccacccac gaaggctggt caaagctgaa cttcaagccg
    14581 ttggcgggca ccagggctac ggcggaaaca ggcgcaggat cagccggtgg tgacgctgtc
    14641 gcgcccgcag aagaaaccgt tgcagccgga ggagtaagcg gagtgagaga aacgggtact
    14701 gccacggacg ccgttgtccc ggatgcggca ggcgcaccga ctttctcgac gacaacctcg
    14761 ctggattgag tggccgattt gagtgcggag agggctgatt gccacaaatc ctgcgaaaca
    14821 agaaagtacg acaagacagc cagtagcaga acgaaaagac ccgcgaacac cgtgaagaag
    14881 tcgcgtcgat ccgtcttccc gtcgttttgc aggtttacgg tggggccggt tggaatgtcg
    14941 agttccggag cctgtggcat atgaagccgg tcaagctcag tcataagctg atccgaattc
    15001 aggttgagca ggcgggcgta attgcgcacg aaaccccgga tgatcgtttt tccgggcaaa
    15061 ttcggccagt cgtcagcctc aagtgccttc acttgcctaa aactgagctt gatcgctttg
    15121 gcaacatcat ccgcagtcag accccgggct tcacgggcag cacggagttg ctgaccgacg
    15181 ctcagcacca cggagtcctg agtcgcttca aacgaccctg gagtctgggt catatcgggt
    15241 tcgacattgg cctcgttgtc cgatcccgaa gacggaacat ttacgctggt cattcgaagt
    15301 tgcctttcag gaattcctga tattctggtg aatcaggata tttccgccgc aactgagccg
    15361 ccagactgat ctcgtccgag ctattaccca agcggcgttc aaccctcaaa aacagccaaa
    15421 gcgcctccgc gttaggattg ctcaaacgaa ccactttctt cagatgttcc ttggcggcat
    15481 cgtagttgcc acgcttgtag ctgatattgg ccaattgcag cagggcttgc ggattgtccg
    15541 gcatatagcg caaggtcttg cgaatgtaat cctctgccaa atccagctcg ttgatccttg
    15601 catagcatgt accggcattc aggtgagcta acgcaggcgt ctgatacaag gggtcattta
    15661 ttgctcgccg gaagtattca atggactcct tctctttccc ggtattacag agaaaccagc
    15721 catagttatt actgatctga ggatccttgg gagccaggtc caaagcacgc tgaaaatcct
    15781 tttcagcgga cgcaaactcc ttgatttggt acaacacgag ccctcgcgta ctgtaggctt
    15841 gcgcgtagtt tgcatcgatc gaaatggcaa tggtcagctc ctcgagcgcg acaatcaaat
    15901 cgccatttcg aaaatacagc gaacccagtt ccgtatgcag cttggcgcgc gtgtgtgcat
    15961 ccttcggcgt cattgatgaa agcggttcga ctggcggagc aaccgactgt tgagccaatg
    16021 cggacgaaac cgccaccagg gtcagtgcca cagctcgaaa acagcaagaa gcaaaaatcc
    16081 ggtgaagtac tttcatcgtg taacctcgtg aagatcgata atccggcgtg ccgaacgacg
    16141 cgtcctgtcc agcacttggc cggccaactg gccgcaagcc gcatcgatat cgtcaccacg
    16201 cgtcttgcgt gttgtggtga ccacaccggc attcatgagt acctcggcga agcgtcggat
    16261 gcggtaagcc ggcgaacgac gatagggtga gcccggaaac ggattgaacg gtatcaggtt
    16321 gatcttgcag gacaccttgc gcgtcagatc aagcaactcg tacgcctggc tatcagcgtc
    16381 atttacgccg tcgagcatga catactcaaa ggtaacaaaa tctcgtgggg catactgaag
    16441 ataacgattg catgcggcca tcagttcacc caggggatat ttctggttga tcggcaccaa
    16501 ttcgttgcgc agccggtcat tcggagcatg cagcgatacg gcaagagcaa ccggacactc
    16561 ctcgcccaga cgatccatcg ccggtaccag accggaagtg gaaacggtca cccggcgacg
    16621 cgacaacccg taagcattgt cgtccagcat caaacgcaac gcggtcaccg tactttcaaa
    16681 attggcgagg ggttcgccca tacccatcag cacaacattg ctgacgatgc gttcgtcctt
    16741 cagcccggtt tcgagggccc gctccgcctg ccagagctga ccgataatct ctgccaccgt
    16801 caggttgcgg ttgaagccct gcttgccggt cgaacaaaag gaacaatcaa gcgcacaacc
    16861 cgcctgggtc gaaatgcata atgttcctcg gtcatcttcg ggaatgaaaa ctgtttcgac
    16921 ggcattgcca ccaccgacat caaacaggaa ttttcgcgtg ccgtcgtccg agagcttgtc
    16981 tgaaacaatg gttggcggaa tgacggatgc cactgccttg agcttttcac gcaggctctt
    17041 ggcaatatcg gacatggcat cgaaatcact ccgcccaaaa cgatgaatcc agcgcagcac
    17101 ctgccttgca cgaaacggct tctcgccata ttcggcgaag agagcggcca ggccggccgc
    17161 atcgtaatcg agcaaattga cagacatccc gaaaatctac ttcagcttag cggccagagt
    17221 agaccgacat tgccgggaag aagaaggcga tttcgttctt ggcggtttct tcgccatcgg
    17281 aaccatgtac cgcattggcg tcgatcgatt cggcgaaatc ggcacgaatt gttcccggat
    17341 cggccttctt cggatcggta gcgcccatca ggtcacggtt cttggcgatg gcgctctcgc
    17401 cttcgagcac ctggatcatg accggaccgg aaatcatgaa ggaaaccaga tccttgaaga
    17461 acggacgctc cttgtgcacc gcatagaatt cgccggcctc ctgcggggac aaccaggcca
    17521 tttttgaagc gatgatcttc agaccattcg tttcaaagcg ggaatagatc ttcccgatca
    17581 cgtttttggc aactgcatcg ggcttgatga tggacagggt gcgttcgata gccatgaatc
    17641 aattctccat aaagaaagaa gttggaaaag acagcaaaaa acgactgcat gaactggaaa
    17701 ccctcaattg taccaacact ccaccgggat tgcctaaacc gagtgttccg agccacaaat
    17761 gaaaacgcgc gcaggcctta cctgcgcgcg tttgaacttc tgaagaaccc gggatcgcta
    17821 catcattccc agcatcgtcg gcagccaggt cgtaatcgcc gggacgtagg tgattatcat
    17881 gaggtacacc agcatcgtca gcagccaggg ccagacggcc ttggagcagt cggtcaggcc
    17941 catcttggcg attccgctgg ccacgtacag attcaggccg accggcggcg tgatcatgcc
    18001 gatctccatg ttcaccgtga tcatcacccc gaagtggacg ggatcgatgc ccagtttcat
    18061 ggcaatcggg aacaggatcg gcgccgtgat caggatgatc gacgagggct ccatgaagct
    18121 gcccatcacc agcagcagga tgttcaccgc aatcaggaag ccgaccatgc cgaccccgga
    18181 atgcgtcagc cattcggcga tgccctgcgg tatctgctcg ttggtcagga tgaaggagaa
    18241 caacaccgca ttggtgatga tgtagagcag catcgcgctc atgttcgccg agttgagcag
    18301 gaccttcggc acgtccttca tgccgatgtc cttatagacg aatacggcga taaagaaggc
    18361 gtacaccgcg ctcatggccg ccgcctcggt cggggtgaac aggccgctgt agatgccgcc
    18421 catcaccacc acgatcagca gcaggcccca gatcgccttg cggaaagcat cccagcgctc
    18481 cgcccatgaa cgcggctgct cgcgcggata gttgcccttc ttggcctcat accaggtggt
    18541 gaagccgagg aacagcgcca gcatcaaacc ggggatcacg cccgccatga acagcgcgcc
    18601 gatcgaactg ttggtcgaaa cggcgtacat caccatgacg atcgacggcg gaatcaggat
    18661 gccaagcccg cccgacgagg cgatgacgcc gacgccgaac ttcttcgggt agcccgccct
    18721 gaccatggcc ggcagcagga tcgagccgat cgccaccacg gtggcgggcg aagaacccga
    18781 taccgcggca aacagcgcgc aggcaaacac cgcgctgagg cccaggccgc cgcgcaaatg
    18841 gccgaccatc gacgtcgcga agaagatcat gcgtttcgcc acgccgccat gcgtcagaaa
    18901 gttgccggcg aggatgaaga aggggatcgc catgatctcg aacttctcga tgccggtgaa
    18961 cagcttgagc gcaacggagt cgagcggcgt ttgcgtgaag gtgaacagga aggtcagtac
    19021 cgtcagaccg agcgagatgg agaccggcat tcccgacagc atcaggaagg tcagcagcac
    19081 gaagatgatc acggcgctca tttcggttct cccttgtcag ccgccgtccc gccatcgccg
    19141 acttgctgtt caggatgacg cttcttgtac ttgagatcgt gcatgtggag atcgtcgtcc
    19201 atgttgtagg ggttgatgtc caccggcacc tcgtcctcgt caacgccgtc gacgtggcca
    19261 tgatcgtgat gcggcagttc gccggtacgg atgaagccga cggtgacctg gaggaaacgg
    19321 aagcacatca ggtaggaacc cagtgggatg gcgctgtata cgatccatgt ctgccattcc
    19381 aggtcgggcg ttgtcggccc ggggtacaag tcgccggtgg ccaggcccaa ggcgttgaag
    19441 atcgcgtagt gggcaccgtt ctcccagacg aagtgcgccc ccagggtgcc gacgatgccg
    19501 gtaaagaacg ctccggccga catgccgaag acgatgaatt tggctcgcgt ccggcccgac
    19561 aggctgttga tcagcacgtc gacgccaacg tgaatgccgg tgcgcacgcc atacgcggca
    19621 ccgaacttgg ccatccagac gaacatgatg atgcacagct cctgcgccca gctcagattg
    19681 agactcagca accagtcctg aaccaccgga atcggcacgc ccgctgcata gcgatgcatc
    19741 accgcggtga agatgatcaa ggtggcgccg ccaatgagga aggtgatcag ccattcctcc
    19801 aggtgatcca gaaatttcat gattccagcc cc
//
LOCUS       DAORPM010000011        18544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_534_length_18544_cov_12.522256, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000011 DAORPM010000000
VERSION     DAORPM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 18544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18544
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_534_length_18544_cov_12.522256"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /gene="recO"
                     /locus_tag="PLD53_01120"
     CDS             complement(<1..586)
                     /gene="recO"
                     /locus_tag="PLD53_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRC59534.1"
                     /translation="MHRNKVDGQPAFVLHTYAFRETSLIVEVFSRDFGRVALLARGAR
                     RPRSPMRGVLLAFQPLELAWAGRGEVQTLIRAEWQGGQPLLSGKALFCAYYLNELLMQ
                     LLPREDAHLRLFSVYGETLRRFSMTFRESDLRCFERSFLQELGYGLTLEKDVDGVPID
                     IAGHYAYRIENGPVKLQRPGSSALSISGKTLIDLS"
     gene            complement(611..1495)
                     /gene="era"
                     /locus_tag="PLD53_01125"
     CDS             complement(611..1495)
                     /gene="era"
                     /locus_tag="PLD53_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRC59535.1"
                     /translation="MSELKSGTIAIVGRPNVGKSTLLNRLVGEKISIVSRKAQTTRHR
                     IMGILTQPDAQYVFVDTPGFQTRHTNALNRAMNRGVTQALAEIDVVVLVVDADRFDAK
                     DKAVIQLLTPERPVILAVNKIDQIKDKTRLLPILANLATQYSFAAIVPISAATGSQLD
                     DLLAEIRKQLPHKELLYGEDEITDRSERFLAAEFIREKLFRLTGDELPYAATVEVEKF
                     EIDGALRRISAAIVVDRQAHKGIVIGKSGETLKRIASEARQDMERLFDGKVFLEVFVK
                     VKSGWSNDERLLKSLGYE"
     gene            complement(1492..2160)
                     /gene="rnc"
                     /locus_tag="PLD53_01130"
     CDS             complement(1492..2160)
                     /gene="rnc"
                     /locus_tag="PLD53_01130"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRC59536.1"
                     /translation="MTNRLEQALGHLYSDQSLLLTALTHRSHSWPHNERLEFLGDSIL
                     NGVIARQLFDCFPDLPEGDLSRLRANLVRQESLHQLALSLSLGEFLRLGDGELKSGGR
                     QRPSILADALEALFGAIWLDAGFDAASAVIVRLYAEKLATIVPGQPIKDAKTRLQEAL
                     QGRRLPLPKYSLASTEGEAHAQQFSISCQIDALNIRTQGSGGSRRAAEQMAAERALED
                     IQHA"
     gene            complement(2169..2522)
                     /locus_tag="PLD53_01135"
     CDS             complement(2169..2522)
                     /locus_tag="PLD53_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HRC59537.1"
                     /translation="MKNQRGVAISGLLLWGFVIVFVALLGMKVVPEYMDYFQILKSVK
                     SISKDSSGQTVADIRNAYDRYADINNIKTIAGTDLDISKEDNTVVIAFAYETRIPLFY
                     NVSLLIDFRGSSSGN"
     gene            complement(2535..3323)
                     /gene="lepB"
                     /locus_tag="PLD53_01140"
     CDS             complement(2535..3323)
                     /gene="lepB"
                     /locus_tag="PLD53_01140"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRC59538.1"
                     /translation="MNFALILFCLLLVSGAIWVVDIFVLRKFRAADAKDPWWVEYGAS
                     FFPVILIVFVLRSFIVEPFKIPSASMLPTLHVGDFILVNKFTYGIRLPVVNKKIIDIN
                     EPQRGDVMVFRYPEDPSLDYIKRVVGVPGDKVAYQNKRLTINGKPVETTRVDDYLHPE
                     RLYYSKQFVEKSDGVEHHTLNDVDAPDFVSNATQFPYRNDCLYNNAGLICTVPAGHYF
                     MMGDNRDNSRDSRFWGFVPEENIVGKAFFIWLNFGDLKRIGSFK"
     gene            complement(3335..5125)
                     /gene="lepA"
                     /locus_tag="PLD53_01145"
     CDS             complement(3335..5125)
                     /gene="lepA"
                     /locus_tag="PLD53_01145"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRC59539.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRIIHLCGGLSDREMEAQVLDSM
                     DIERERGITIKAQTAALQYKSHSGAIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIELGVEVLPVLNKIDLPSAMPDNARQEIEDVIGIDASHAIL
                     ASAKTGLGVDEILEAIIERIPAPQGDPDAPLKALLIDSWFDNYVGVVMLVRVIDGTLR
                     QKNRIRLMASDSTHLCEQVGVFTPKAVPRETLSAGEVGYIISGIKELQAAKVGDTVTS
                     AENPAKEALPGFKEIKSQVFAGLYPIESNQYDSLRESLEKLKLNDASLHYEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDQDLISTAPTVVYEVLMRDGSIVQVENPAKLPE
                     PSKVEEVREPIITITVFVPQEYLGNVITLCNQKRGNQVDMTYHGRQVQLIYEMPMAEV
                     VMDFFDKLKSVSRGYASLDYDFKEFRAADVVKLDILINGEKVDALSMIVHRANSIYRG
                     RELASKMRELIPRQMYDVAIQAAIGSNIVARENIKAMRKDVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAVLRVGK"
     gene            complement(5219..5461)
                     /locus_tag="PLD53_01150"
     CDS             complement(5219..5461)
                     /locus_tag="PLD53_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRC59540.1"
                     /translation="MTKLTLISRTYCHLCHEMELALLPLAAEFGVDVDVVDVDADPRL
                     EALYDELVPVLLHEGTELCHYRLEDAKLRDYLSKIR"
     gene            complement(5463..6905)
                     /locus_tag="PLD53_01155"
     CDS             complement(5463..6905)
                     /locus_tag="PLD53_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRC59541.1"
                     /translation="MKQLVTAFFIAFISLTAQAQNRALPDFTELVEKQGPAVVNISTT
                     QVVRGFGRGVQPFPFDEDDPTSDFFRRFFPRQPGMPGAPGVPREFESRSLGSGFIISA
                     DGFILTNAHLVESADEIFVRLTDKREFKAKVIGSDKRTDVALIKIVATNLPTVRLGDP
                     EILRVGEWVVAIGSPFGFDNSVTAGIISAKGRSLPQENFVPFLQTDVAINPGNSGGPL
                     FNMKGEVVGINSQIYSRSGGFMGISFAIPIDVAMDIQNQLRVSGRIRRGRIGVVIQEV
                     TKELAESFGLSKPQGAVVNAVERGGPADKAGVEPGDVILKFDGKPVVNSGDLPRIVGS
                     SKPGASAPMQVWRKGSVRDLTVVVGETPEEKAMARNGKNPRSTEQAANRLGLVVSELS
                     PERQRELRISGGLLVEDVRGNVVRADLQPGDIILALIARGESSEIRSVDQFNKLLAQF
                     DKASTITLLVKRGELQTFITIKGLPEKKAG"
     gene            complement(6970..7386)
                     /locus_tag="PLD53_01160"
     CDS             complement(6970..7386)
                     /locus_tag="PLD53_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HRC59542.1"
                     /translation="MIDVPGKIFALDGDYAIVRVEESGCGRCHETGGCGGTNIATLFC
                     HAPRSFRVLNPGNSAIGDSVSIVIDEGAVRRSAALAYGLPLLLLFIGALGGSALAGET
                     GAIVGSVSGLFFSWLAMRFALLRGTPDQRSQPYIRS"
     gene            complement(7454..8053)
                     /gene="rpoE"
                     /locus_tag="PLD53_01165"
     CDS             complement(7454..8053)
                     /gene="rpoE"
                     /locus_tag="PLD53_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HRC59543.1"
                     /translation="MNEREIDQELVKRAQAGDKQSFDRLVEKYQRKLGRLLSRFVRDH
                     AEVEDVAQEAFIKAYRALPSFRGESAFYTWLYRIGINTAKNYLVAKGRRAPTSTDFDA
                     DQAESFDDADQLRDNNTPERLLQSKQLGQTVNAAMEALPEELRNAIVLREIEGLSYEE
                     IAEEMNCPIGTVRSRIFRAREAVAEKLKPLLDTATDHRW"
     gene            8210..9847
                     /gene="nadB"
                     /locus_tag="PLD53_01170"
     CDS             8210..9847
                     /gene="nadB"
                     /locus_tag="PLD53_01170"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRC59544.1"
                     /translation="MVLRFDILVIGSGLAGLSAALRLAQTCKVGLISKRALVDSASSW
                     AQGGIAAVLDSQDSVEAHIRDTMTAGAFLNDQETTRFVIENGRRVIDWLVEQGVPFSR
                     DEAGFHLNREGGHGARRVIHVADATGLSVQSTLIRKIREHPNITVLERHIAIDLITGE
                     KLGHADKRCYGAYALDSDSGEVLTIGALHTLIATGGAGKVYLYTTNPDTSTGDGIAMA
                     WRAGCAVANMEFIQFHPTCLYHPQAKSFLISEAVRGEGGLLLLPDGTRFMPQHDSRAE
                     LAPRDVVARAIDFEMKKLGLDCVYLDISHKPVSFLAEHFPNIMTRCLELGIDISREPI
                     PVVPAAHYTCGGIVTDLRARTSIPGLYAAGEASCTGFHGANRLASNSLLECLVFAEAA
                     VKNILEQPRIDLPDIPEWDASRVVDPDEEIVISHNWDELRRFMWDYVGIVRTTKRLER
                     AQNRIHLLAREIDEYYANFRVSHDLIELRNLVVTADLIVRCALLRRESRGLHFSRDYP
                     ETLQKAKNTRIKRSSRRAPYSEAKQNLDETRSAETTK"
     gene            complement(9903..10328)
                     /locus_tag="PLD53_01175"
     CDS             complement(9903..10328)
                     /locus_tag="PLD53_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59545.1"
                     /translation="MQLPVFIELHRSQRLSLLLLLFHTIAAGCVIALPWPLLLRSLLI
                     VVIGLSLWHALRPPRIVGLHLTGRNALECVLVDGNRVGVTALPDSTVFLRLIVMRLRI
                     GEEKRVCNLALLPDHMSSDQFRMLRLWLRWHAVPVVDTK"
     gene            complement(10346..11584)
                     /gene="fabF"
                     /locus_tag="PLD53_01180"
     CDS             complement(10346..11584)
                     /gene="fabF"
                     /locus_tag="PLD53_01180"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRC59546.1"
                     /translation="MTRRRVVITGLGVVSPVGNTVEQAWKNVLAGRSGIDRITRFDAS
                     SFPVQIAGEVRDFDITQYLPAKDARRMDLFIHYGMAAGIQAIKDAGLEAHPDNAERIG
                     VSIGSGIGGLPMIESTRDEFVASGVRKISPFFVPGSIINMISGNLSIMYGYKGPNIAL
                     VSACSTGTHSIGDAGRLIEYGDADVMIAGGAECTVSPLGLGGFCAARALSTRNDDPTT
                     ASRPWDKDRDGFVLAEGAGVMVLEEYEHAKARGARIYGELAGFGMSADAHHMTAPCED
                     GEGAARCMTNALRNSQLNLQDVQYLNAHGTSTPLGDTAETVAVKRAFGQHAKNLVINS
                     TKSMTGHLLGAAGGIEAVFSVLAIHEQVSPPTINIFDQDELCDLDYCANEARNMKIDV
                     AISNSFGFGGTNATAVFKRV"
     gene            complement(11698..11934)
                     /gene="acpP"
                     /locus_tag="PLD53_01185"
     CDS             complement(11698..11934)
                     /gene="acpP"
                     /locus_tag="PLD53_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRC59547.1"
                     /translation="MENIEQRVRKIVAEQLGVNEADIKNESSFVDDLGADSLDTVELV
                     MALEEEFECEIPDDEAEKITNVQQAIDYILSHKK"
     gene            complement(12053..12793)
                     /gene="fabG"
                     /locus_tag="PLD53_01190"
     CDS             complement(12053..12793)
                     /gene="fabG"
                     /locus_tag="PLD53_01190"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRC59548.1"
                     /translation="MLNGQVALVTGASRGIGQAIALELGKLGATVIGTATSNEGAASI
                     SEYLATASIAGEGAALEVCDQAQIDALVTRIEKTYGATTILVNNAGITRDNLSMRMKD
                     DEWDAVLDTNLKAVFRLSKAVMRGMMKVRQGRIINITSIVGHSGNPGQANYCAAKAGV
                     AGMTRSLARELGSRNITVNCVAPGLIDTDMTKSLDEEHRKALVANIPLGRLGLPEDVA
                     ASVGFLASPGAAYVTGTTIHVNGGMYLD"
     gene            complement(12802..13743)
                     /gene="fabD"
                     /locus_tag="PLD53_01195"
     CDS             complement(12802..13743)
                     /gene="fabD"
                     /locus_tag="PLD53_01195"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRC59549.1"
                     /translation="MAFAFVFPGQGSQSVGMMAAYGDAAVVRATFDEASATLGQDLWE
                     LLSQGPAEALAQTVNTQPVMLTAGIATYRLWLDKGGRLPAVVAGHSLGEYSALVAAGV
                     IDFRDAVPLVRLRAAAMQEAVPVGTGAMAAIMNLDDDRIQEACAEAADEVGNGEVVER
                     VNFNAPGQTVIAGSKAAVERACEGCKARGAKRALLLPVSAPFHSSLIRPAAVKLGARL
                     AELNFAEPKIQVVNNVDVAIESDPARIKDALIRQAFSPVRWVETIQKIASIGITTVAE
                     CGPGKVLAGLTKRCSGGLNGLALADLAAIEAAIPSME"
     gene            complement(13755..14714)
                     /locus_tag="PLD53_01200"
     CDS             complement(13755..14714)
                     /locus_tag="PLD53_01200"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRC59550.1"
                     /translation="MFARITGVGSYLPGQPVSNTDLLNRGVETDDEWIFSRTGIRTRH
                     LADSSCTSSDLALEAGRRALAAAGTDAGQLDLIILATSTPDFIFPSTACLLQAKLGNQ
                     GAMAFDVQAVCSGFAYALTVAEKFIRSGSHKKALVVGAEVFSRILDWEDRTTCVLFGD
                     GAGAVVLEASEAPGILATAAHADGSHHGILSVPGSVCGGKVIGDPFLRMDGQAVFKFA
                     VRVLTDVAKECCEGAGISPSEVDWVIPHQANIRIIESTARKLGLGMEKFIVTVDQHGN
                     TSAASIPLALDVAVRDGRIQRGHKVMLEGVGGGFTWGAVLLEF"
     gene            complement(14716..15729)
                     /gene="plsX"
                     /locus_tag="PLD53_01205"
     CDS             complement(14716..15729)
                     /gene="plsX"
                     /locus_tag="PLD53_01205"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRC59551.1"
                     /translation="MTVTVAIDCMGGDFGPAVTVRAARDFVENHDDVSVILVGIEEKI
                     SPFLARSADSRIAIHHASEIVAMDEAPALALRNKKDSSMRVAINLVKQGQADACVSAG
                     NTGALMAISRFVLKMLPGIDRPAICAILPTAKGHSHVLDLGANVDCSPEHLLQFGIMG
                     ASLVAAMEHKDRPTVGILNIGAEAIKGNDVVKRAAELLRESGLNFVGNVEGDGIYKGE
                     AEVIVCDGFVGNVALKASEGLAQMLATFLKREFNRSLLTKLVALIALPVLNNFKKHVD
                     HRRYNGATLLGLKGIVVKSHGSADVFSFANAIERAAEEARTGVLSRITERVALQQPLQ
                     EIA"
     gene            complement(15799..15978)
                     /gene="rpmF"
                     /locus_tag="PLD53_01210"
     CDS             complement(15799..15978)
                     /gene="rpmF"
                     /locus_tag="PLD53_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRC59552.1"
                     /translation="MAVQQNKKSPSKRGMRRAHDFLTNPPLAVEPTTGEVHLRHHISP
                     SGYYRGKKVTKGTGE"
     gene            complement(16003..16437)
                     /locus_tag="PLD53_01215"
     CDS             complement(16003..16437)
                     /locus_tag="PLD53_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRC59553.1"
                     /translation="MQGKLLIADLARVLDKLAGSAGYLSFRIEGRMGARNRPQLQLQL
                     DGVLSVCCQRCLEGIDYPVEVRSVLEFVDDEELTQEEIEDDSMDFLPAQKELDVVALI
                     EDEIILDLPSAPRHKSCALPDIKAGKVKISPFAVLQGFKGKA"
     gene            16610..17194
                     /locus_tag="PLD53_01220"
     CDS             16610..17194
                     /locus_tag="PLD53_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRC59554.1"
                     /translation="MPSVQIVLSSTSLFRRELLARLGLPFETANPNVDETARHGETPE
                     TTALRLSEDKARAVARQYPEALIIGSDQVAYLDGQVFGKPGTHENAARQLQTMRGRTV
                     NFYTGLCLLNAKSGKVQLRGVPTLVTFRNLTSDEIENYLRKEKPYNCAGSAKSEGLGI
                     AIIAKMEGEDPNALIGLPLIALCDFLRNESVYAI"
     gene            17228..17938
                     /locus_tag="PLD53_01225"
     CDS             17228..17938
                     /locus_tag="PLD53_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRC59555.1"
                     /translation="MEKGRLYLVPVPLGPVAAESVLPAPVLDCARKLKHFVAESAKSA
                     RAMLKSLPSDYPLQLIEIRELNEHTSLNALPDLLAPLLAGMDVGLISEAGCPAVADPG
                     ANLVALAHREGIQVIPMIGPSSILLALMGSGLSGQHFAFHGYLPTNQEQRQKRLRELE
                     KDSRQGGKTQIFIETPYRNCQMLETLISVCAQNTKICVATDLTLGSESILTRTPAEWR
                     RQGVPSINRRPTVFLIQA"
     gene            complement(17967..>18544)
                     /locus_tag="PLD53_01230"
     CDS             complement(17967..>18544)
                     /locus_tag="PLD53_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRC59556.1"
                     /translation="RTKHPQIPLYVVVEDVCASGGYYVASAADKIFVDKASIVGSIGV
                     LMDGFGFTGTMDKLGVERRLLTAGVNKGFLDPFSPQDERQKEHAQILLGEIHRQFIEV
                     VRNGRGNRLKETPDMFSGLMWTGSQSIKMGLADDFGTVDSVARDLIKAETILDYSVKE
                     NIAERFAKRLGAETGQSIVFRLFGSNRTGLH"
BASE COUNT         4270 a         5249 c         4840 g         4185 t
ORIGIN      
        1 gcgacaggtc aatcaaggtc ttgccgctga tcgacaatgc tgaactgccg gggcgctgga
       61 gctttaccgg gccattttcg atccggtaag cataatgccc ggcaatatcg atcggcacac
      121 cgtcgacgtc cttttcaaga gtcagtccgt aacccaattc ctgcaggaat gaccgctcga
      181 agcagcgcag atccgactct ctgaatgtca ttgaaaagcg tcgcaatgtt tcaccataga
      241 ccgagaaaag gcgcaaatgg gcatcttcgc gcggcagaag ctgcatcaac agctcgttga
      301 ggtagtaagc gcaaaacagc gccttgccgg acagcaaggg ttgcccgccc tgccactcgg
      361 cacgtatcag ggtttgcact tcgcctcgac cagcccaggc cagttcgagc ggttggaagg
      421 cgagcaatac gccacgcatg ggggaacgcg ggcggcgggc tcctcgcgcc aacagggcaa
      481 cacggccaaa gtcgcgcgag aacacttcga cgatcaggct ggtttcgcgg aaagcgtagg
      541 tgtgcaacac gaaagcaggc tggccgtcga ccttgttccg gtgcatcacc tcagacgccc
      601 tctccgcgat ctactcgtag ccaaggctct tgagcaaacg ctcatcattg ctccaccccg
      661 acttgacctt gacaaaaact tcaagaaata ccttgccgtc aaacaggcgt tccatgtcct
      721 ggcgcgcttc cgaggcgatt cgcttcagag tctcgccact tttgccgatg acaatcccct
      781 tgtgtgcctg gcgatcaacg actattgcag cactgatacg gcgcaatgct ccgtcaatct
      841 cgaacttttc cacttcgacg gtggcggcat agggcagttc gtcaccggtc aatcggaaga
      901 gtttttcgcg aatgaattcg gcggcgagaa atcgttcgct cctgtcggtg atctcgtctt
      961 caccgtacag cagttccttg tgtggcagtt gtttgcgaat ttcggccagc aggtcatcga
     1021 gctgactgcc agtcgccgcg ctgatcggaa cgatggcggc aaacgaatac tgcgtggcga
     1081 ggttggcgag aatgggcaag agccgagtct tgtccttgat ctggtcgatc ttgttgacgg
     1141 ccagaatgac cggtcgttcc ggtgtcagga gttggatgac cgccttgtct ttcgcgtcaa
     1201 aacgatccgc atcgactaca agaactacaa catcgatttc ggccagcgct tgcgttacgc
     1261 cgcgattcat cgcccggttg agcgcattgg tatgccgcgt ctgaaaccct ggggtatcga
     1321 caaatacata ttgggcatca ggctgcgtca gaatacccat gatgcgatgc cgcgttgtct
     1381 gtgccttgcg cgagactatg ctgatttttt caccgaccag tcggttgagt agagtcgact
     1441 tgccaacgtt cgggcgaccg acgatggcaa tcgtgccgga tttcaattca ctcatgcgtg
     1501 ttgtatatcc tccagcgcgc gctcggccgc catttgctcc gctgcccgac gactgcctcc
     1561 actgccctgg gtgcgaatgt tcaaggcatc gatctggcag gaaatactga attgctgcgc
     1621 gtgtgcttcg ccttcggtgc tggccagtga gtattttggc agaggcaggc gtctgccttg
     1681 caatgcttcc tgaaggcggg tcttggcgtc cttgatcggc tgcccgggaa cgatggttgc
     1741 aagcttttcg gcatataagc gcacgatgac tgcgctggcc gcatcgaatc cggcatccag
     1801 ccagatggca ccaaacaagg cctcaagagc gtcagcaagt attgacgggc gctgtcggcc
     1861 gccgctcttc agctcaccgt ctcccagccg cagaaactct ccaagcgata gcgacagagc
     1921 cagttgatgg aggctttcct gtcggacaag attggcgcgc aatcgtgata ggtcgccctc
     1981 tggcaaatcc gggaaacaat cgaagagttg gcgagcaatg accccattga ggatggaatc
     2041 accaagaaac tcgagccgtt cattgtgcgg ccaactgtgg ctgcgatgcg tcagtgcagt
     2101 cagcagtagg gactgatcgg aatacagatg gcctagtgct tgctcaagtc gattggtcat
     2161 tcggccgcct aattccctga cgatgagccc cggaaatcga tcaacaaact gacgttgtag
     2221 aacagaggaa tgcgtgtttc gtatgcaaac gcaatgacca cggtgttatc ctctttggaa
     2281 atatcgaggt cagttccggc aattgtcttg atattgttta tgtcggcgta cctgtcatag
     2341 gcattacgaa tatcggcaac tgtttgccct gacgaatctt tcgatatgga tttgacactt
     2401 ttcaaaatct gaaagtaatc catgtactcc ggtacaactt tcatgcccag caaggcaaca
     2461 aacacgatga caaagcccca cagcaacaac cccgaaatcg ccacaccacg ctgatttttc
     2521 atagcaaatt ccctctattt aaatgagcca atccgtttca gatctccaaa gttgagccag
     2581 atgaaaaagg ccttgccaac gatgttttct tccggaacaa acccccaaaa acggctatca
     2641 cggctgttgt cgcggttatc acccatcatg aagtagtgcc cggccggcac tgtgcagatc
     2701 aaacctgcgt tattgtagag gcaatcattc cgatatggaa attgagtggc atttgaaaca
     2761 aagtcagggg catcgacatc gttgagcgta tggtgctcga ctccatcgga tttctcgaca
     2821 aactgcttcg aatagtagag ccgttccgga tgaagataat cgtccactct cgtggtttct
     2881 accggcttcc cgttaatcgt gagccgcttg ttctggtagg ccaccttgtc gcctggaacg
     2941 ccgacaacgc gcttgatata gtccagtgac ggatcttccg gatagcggaa gaccatgacg
     3001 tcacctcgct gcggctcgtt gatatcaatg atcttcttgt tgactactgg caatcgaatc
     3061 ccgtaggtaa atttgttgac cagaatgaaa tcgccaacat gaagcgttgg caacatcgat
     3121 gccgacggga tcttgaacgg ttcaacaata aaagaacgca gcacgaaaac gatcaggata
     3181 accgggaaga agctcgcgcc atactccacc caccagggat ccttggcgtc tgccgccctg
     3241 aacttcctca agacaaaaat atccaccacc cagatggcgc cactgacaag cagtaggcaa
     3301 aaaaggatca acgcaaaatt cacacttgta ctccctactt tccgacacgc agcacagcaa
     3361 gaaaggcttc ctgtggaatc tcaaccgacc cgacctgttt catccgcttc ttgccggcct
     3421 tttgcttctc gagcaatttc ttcttgcgcg tgatgtcgcc accgtagcac ttggcaagta
     3481 cgtccttgcg catggctttg atgttttcac gtgcgacgat gttcgaccct atcgccgcct
     3541 gaatggcgac gtcgtacatc tggcgtggaa tcaattcgcg cattttggag gcgagttctc
     3601 gtccccggta gatcgaattt gcccgatgca caatcatcga caaggcatca actttctcgc
     3661 cattgatcag gatgtccagc ttgacgacgt cggccgctct gaattccttg aaatcgtagt
     3721 cgagcgaggc atatccgcgc gagacggatt tcaacttgtc gaagaaatcc atgaccactt
     3781 cagccatcgg catctcatag atcaattgaa cctgacgccc gtggtaggtc atgtccacct
     3841 gattgccacg cttctggttg cacagggtaa tgacgttgcc gaggtactcc tgtggaacaa
     3901 agacggtaat cgtgatgatc ggttcgcgaa cctcttcgac ttttgacggt tccggaagtt
     3961 ttgccgggtt ttccacctga acgatgctgc cgtcgcgcat cagaacttca tagacgaccg
     4021 ttggcgcggt agagatgaga tcctgatcga attcgcgttc caaccgctcc tgcacgattt
     4081 ccatgtgcag caagccaaga aacccgcaac gaaaaccgaa gccaagcgcc tgcgatactt
     4141 caggttcgta gtgcagagag gcgtcgttga gcttgagttt ctccagcgat tcgcgcagcg
     4201 aatcgtactg gttggattcg atcggataaa ggccggcaaa tacctgtgac ttgatttcct
     4261 tgaatccggg cagcgcttcc ttcgcaggat tctcggctga cgttacggta tctcccactt
     4321 tggccgcttg cagctccttg atgccggaaa ttatgtaacc cacttcgccg gcgctcaagg
     4381 tttccctggg cacagcctta ggcgtaaaca cacccacttg ttcgcacaaa tgggtcgaat
     4441 cacttgccat caagcgaatc ctgttcttct ggcgcaaggt tccatcgatg acgcgaacca
     4501 gcatgacaac gccgacgtaa ttgtcaaacc atgaatcaat cagaagcgcc tttagcggcg
     4561 catcggggtc tccttgtggt gccgggatac gctcgatgat cgcttccagg atttcatcca
     4621 cgccaagacc cgtcttggca gaagcaagaa tggcgtgtga agcgtcaatg ccaatgacgt
     4681 cctcgatttc ctgacgtgcg ttgtcgggca tggccgacgg aagatcgatc ttgttcaaga
     4741 cgggcagaac ttcgacgccg agttcgattg cggtgtagca gttcgccacc gtctgcgctt
     4801 cgacgccttg cgacgcatcg acaaccagca aagcgccctc gcacgcagaa agtgaacgcg
     4861 acacttcgta ggaaaagtcg acgtgtcccg gcgtgtcgat gaggttcaga ttgtatattg
     4921 cgccgctgtg cgatttgtac tgcaacgcag cggtctgcgc cttgatggtt atacctcgct
     4981 cacgttcgat gtccattgaa tcgagcactt gcgcttccat ttcgcggtcg gacaatccac
     5041 cgcaaagatg gatgatgcgg tcggcaagcg ttgatttgcc atggtcgata tgggcgatga
     5101 tcgagaagtt gcggatgtgt tgcatttgca tcgtgtttgc actgccgcgg caaggccgcg
     5161 cctgaaaaaa agggtgccgg cgggcaccct ttgtttgatc tgccagaagg cgcggatttt
     5221 agcggatttt gctcaaataa tcacggagtt tggcatcttc gaggcggtaa tgacagagct
     5281 cggttccctc atgcagcagt actggaacca attcatcata tagcgcttca agcctggggt
     5341 cggcatccac atcgacaaca tcaacgtcaa ccccgaattc agccgcaagc ggaagcaagg
     5401 ccaactccat ttcgtggcag agatggcaat acgtccgcga gatcagcgtc agttttgtca
     5461 cgtcatccag ccttcttttc gggcagtccc tttatggtga taaaggtctg caactccccg
     5521 cgtttaacca gcaaggtaat ggtggatgcc ttgtcaaatt gtgctagcag cttgttgaac
     5581 tgatcaacgc tcctgatttc ggaactttcg ccccgtgcaa tgagtgccag gatgatatcg
     5641 ccaggttgca aatcggcccg aacgacgttg ccacgaacat cctcgaccag caagcctcca
     5701 ctgattctca gttcgcgttg tcgttcaggg cttagttccg aaacgaccag gcccagtcga
     5761 ttggccgcct gctcagttga cctggggttt tttccgtttc tggccatcgc cttttcttcc
     5821 ggcgtttcac cgacgacaac cgtcagatcc cgcacggagc ccttgcgcca tacttgcatg
     5881 ggcgcactcg ctcctggttt gctggagccc acgattcgcg gcaaatcgcc cgagttgaca
     5941 accggcttgc cgtcaaactt gaggatgaca tcaccgggtt cgactccggc cttatcagcg
     6001 ggtcctccac gttcgactgc attgacgaca gcaccctgtg gtttgcttaa cccgaaggat
     6061 tcggccagct ctttggttac ttcctgaatg acgacaccga ttcggccacg gcgaattctg
     6121 ccgctgaccc tgagttggtt ctggatgtcc attgccacgt caatcgggat agcaaacgag
     6181 atgcccatga acccgccgga tcgactgtat atctgcgaat tgatcccaac cacttcgccc
     6241 ttcatgttga acagggggcc tccggaattg cccggattga tcgccacatc ggtctgaagg
     6301 aagggaacaa aattctcttg gggcaaagag cgtcctttgg cactgatgat gccggctgtg
     6361 acactgttgt cgaaaccgaa gggagagcct atcgctacaa cccactcgcc caccctgaga
     6421 atctccggat cacccaggcg taccgtgggc agattggtag ccacgatctt gatcagcgca
     6481 acatctgtcc gcttgtcgga gccgatgact ttggccttga attcccgctt gtcggttaat
     6541 cgaacgaaga tttcatctgc cgattcgacc agatgagcat tggtcagaat gaaaccatcg
     6601 gcactgataa tgaaaccgga acccagcgag cgactttcga actcccgggg aacgccaggc
     6661 gcaccaggca ttccgggttg acgcggaaag aaacgccgaa agaaatcaga cgtcgggtca
     6721 tcttcgtcaa acgggaaggg ctgaacacca cgcccaaaac cacgaaccac ctgtgttgta
     6781 ctgatattta cgacggccgg cccctgcttt tcgacaagct ccgtaaaatc aggcaaggct
     6841 ctattctgcg cctgcgcagt caatgaaata aaagcaatga aaaatgccgt cacaagctgc
     6901 ttcatggtga caaatgcctc ctgtgagtcc gttttgcctg gccggaaatt ctggatcgat
     6961 gaactgaaat caagatctga tatagggctg cgaacgctgg tcgggtgtgc ctcgaagtag
     7021 agcaaaacgc atcgccagcc atgagaaaaa caagccactg accgacccga cgatagcgcc
     7081 ggtttccccg gccagcgccg atccgccaag tgcgccgatg aacaatagca gcagcggcaa
     7141 tccgtaagcg agtgcggcac tacgtcgaac agcgccttcg tcaatcacta tgcttacgct
     7201 gtcgccaatg gccgagttgc cagggttcaa tacacggaag cttcgtggcg catggcaaaa
     7261 caacgtggcg atgttggttc cgccacaacc gccggtttca tgacagcgtc cgcatcccga
     7321 ttcttccact cggacaatcg cataatcgcc atcaagcgcg aaaatctttc caggcacatc
     7381 aatcattcgt attccctgga tcgggtccgc ctgacggtat attgaacatg gcgtgatgac
     7441 gcatcggtcc agtctaccac ctgtgatccg tagccgtatc cagcaaaggt ttcagctttt
     7501 ctgccacggc ctcacgtgca cggaaaatac gcgagcgcac cgtgcctatc ggacagttca
     7561 tctcttcagc aatctcctca tagctcaggc cttcgatttc acgcaataca attgcattgc
     7621 gcagctcttc cggaagcgct tccatcgccg cattgaccgt ttgcccgagc tgcttggact
     7681 gcaacaatcg ctcgggggtg ttgttatcgc gcagttgatc tgcgtcatcg aaggattctg
     7741 cctgatcagc atcaaagtca gttgatgtcg gggcgcgacg accttttgcg acaagatagt
     7801 tcttggccgt attgatgccg attcggtata gccaggtata gaacgcgctc tcgccacgaa
     7861 acgatggcag tgcacgatag gccttgatga acgcctcctg tgcgacgtct tcaacttcag
     7921 cgtggtcgcg aacgaagcgc gatagaagtc gtccaagctt gcgctggtac ttttcaacca
     7981 gccgatcgaa actttgcttg tcgccagcct gtgctcgctt gaccaactcc tggtcaattt
     8041 cgcgttcatt catgctatgt gaattccctg tcggacacga tgttgtgttt ttcgcgagag
     8101 tatagcgcaa ccgctaagcc gacgcggcga tagtggccta tctcgcaaca gggtgaaggg
     8161 cttgacgtgt gcgtgttata gtgagctccc attcttcaag tgctttgtcg tggtgcttcg
     8221 ctttgacatt ctcgttattg gtagcggcct ggctggactt tcagcggcgc ttcgtcttgc
     8281 acaaacctgc aaggtcggcc tgatcagcaa acgtgctctt gtggacagcg cctccagttg
     8341 ggcgcaaggg ggtattgctg cagttctgga cagtcaggat tcggtagagg cgcacattcg
     8401 cgacacaatg actgcgggtg cttttctcaa cgatcaggaa accacacgtt tcgttatcga
     8461 aaacggccgc cgagtaattg actggcttgt cgaacaaggc gttccttttt cgcgggacga
     8521 agcgggtttc catttgaacc gcgagggcgg acatggcgca cgtcgtgtca tccacgttgc
     8581 agatgcaaca ggcctgtcgg tacagtcgac cctgatacgc aagattcggg aacatccgaa
     8641 catcactgtt cttgaacgcc acattgccat cgaccttatc accggggaga agctggggca
     8701 tgccgacaag cggtgctatg gcgcctatgc actcgacagt gacagcggtg aagtgttgac
     8761 tatcggcgca ctacatacac tgattgcgac gggtggcgct ggaaaggttt atctctatac
     8821 gacgaacccg gatacctcta ccggagacgg tattgcgatg gcatggcgcg ccggatgtgc
     8881 ggtagctaat atggagttca tccagttcca tccaacctgc ctttaccatc ctcaggccaa
     8941 atctttcctg atttctgaag cagtacgggg tgaaggtgga ctcctactct tgcccgacgg
     9001 cactcgcttc atgccgcaac atgattcgcg tgccgaactg gcgccgcgcg atgtcgttgc
     9061 cagggcaatc gactttgaaa tgaaaaagct tggcctcgat tgcgtctatc tcgatatttc
     9121 tcacaagcct gtcagctttc ttgccgagca tttcccgaac atcatgacgc gctgccttga
     9181 actgggtata gacatttccc gtgagccgat cccggtagtt cccgctgccc attacacctg
     9241 tggcggcatt gtcaccgatc ttcgtgcccg cacaagcatt ccggggctat acgcagctgg
     9301 cgaggcgtcc tgcactggtt ttcacggcgc caaccggctg gctagcaact cgctgctgga
     9361 atgcctggtt tttgcagagg cagctgtcaa gaatattctc gaacaaccca gaatcgacct
     9421 tccggatatc cctgaatggg atgccagtcg cgtcgttgat cccgacgagg agattgtcat
     9481 ctcacacaat tgggatgagc tgcgtcgctt catgtgggat tacgtgggta ttgtccgcac
     9541 taccaagcgt ctggagcgcg ctcagaatcg cattcacctc ctggcacggg aaattgacga
     9601 gtattatgcg aatttccggg tcagccacga tctgattgaa ctgcgcaatc ttgttgtgac
     9661 ggcagacttg atcgttcggt gtgcgttgct acgccgtgaa agtcgcggac ttcacttcag
     9721 ccgcgactac ccggaaacgc tccagaaagc gaagaataca cgaatcaaga gatcgtcccg
     9781 gcgcgcccca tactctgaag cgaaacagaa tctggatgag acgagaagcg cggaaaccac
     9841 caaatgatgg gccgtcaaat gccaagaggc agacgcctcc tactgattac aagagcgcga
     9901 tttcatttgg tatcgacaac gggaactgcg tgccagcgta accacaggcg aagcatcctg
     9961 aattgatcgg aagacatatg gtcgggaagc aacgcaaggt tgcagacccg cttttcttcg
    10021 ccaatgcgca gccgcattac gatcaatcgg agaaacaccg tgctgtccgg cagcgcagtc
    10081 actccgacac gattaccgtc gaccagcacg cattccagcg cgtttcttcc tgtaaggtgc
    10141 aaaccgacga ttctgggcgg acgcagagcg tgccatagcg ataagccaat gaccacgatc
    10201 agcagtgaac gcaaaagtaa cggccacggc aatgcgatta cgcaaccggc agctatcgtg
    10261 tgaaacagaa ggagcagaag tgaaagacgt tgcgagcggt gcagttcaat gaaaacgggg
    10321 agctgcacgg cttgaacgga atcggctaga cccgtttgaa gacagcggtt gcgttcgtac
    10381 ctccaaagcc gaatgagtta gaaatggcaa cgtcgatctt catgttgcgc gcctcatttg
    10441 cgcaataatc aagatcacac aattcatcct gatcaaagat attgatggtc ggcggggata
    10501 cttgctcgtg aattgccagc accgagaata cagcttcaat gccgccggct gcaccaagca
    10561 ggtggccggt catcgacttt gtcgaattga tcaccaggtt ctttgcatgt tggccaaagg
    10621 cgcgctttac cgctaccgtt tcagctgtat cgccaagagg cgttgaggtg ccatgtgcat
    10681 ttaggtactg cacatcctgc agattcagtt gggaatttct cagcgcgttg gtcatgcaac
    10741 gcgcggcgcc ctcgccgtct tcacaaggcg ccgtcatgtg atgggcatcc gcgctcatgc
    10801 caaagccggc caattcgcca tatattctgg cgccacgcgc tttggcgtgt tcgtactcct
    10861 ctagcaccat cacgccggca ccctcggcca acacaaaacc gtcgcgatct ttgtcccacg
    10921 gacggctggc agtagtcggg tcatcgttgc gcgtggatag ggcacgagcg gcgcaaaatc
    10981 caccaagccc gaggggggaa acggtgcact cggcaccacc ggcaatcatg acatcggcgt
    11041 caccgtattc gatgagacgt cccgcatcgc cgatgctgtg tgttcccgtc gaacaagcgc
    11101 tgaccaaggc gatgttcggc cccttgtagc catacatgat cgacagattg cccgagatca
    11161 tgttgatgat tgaaccgggg acaaagaacg gggaaatctt ccgtactccg gaagcaacga
    11221 attcatcgcg agtactctct atcatcggta atccgccgat gccggaaccg atggaaacac
    11281 cgatacgctc ggcattgtcc gggtgagcct caagtccagc atccttgatc gcctgaatac
    11341 cggcagccat accataatgg atgaataaat ccatacggcg tgcatctttg gcgggaaggt
    11401 attgcgtgat atcgaaatcc ctgacttcgc cggcaatctg cacggggaat gaagaagcat
    11461 cgaaacgggt gatccggtct attccggagc gcccagcaag aacgttcttc caggcttgtt
    11521 caactgtatt accaaccgga gaaaccactc caaggccggt gatgacgact ctgcgacgtg
    11581 tcaaattttt ctccggggga aaacaggaag aaacgctaaa tggagataag agggttcggg
    11641 gaaaaatcct gcagggtagt tccaactaca gccccgcagt tgtgtttcgc tgaaggctta
    11701 tttcttgtgg ctcaagatgt aatcaatggc ttgctgaaca ttggtgatct tctcggcttc
    11761 gtcatccgga atctcgcact cgaactcttc ttcgagagcc atcacgagtt ctactgtatc
    11821 caacgagtct gcgccgaggt cgtcaacaaa agaagattca ttcttgatat ccgcttcatt
    11881 gacgccgagt tgttctgcga ctatttttct gacgcgctgt tcgatatttt ccatctaagt
    11941 gctccttcga aagttcattt atgaaacaaa accgggctat tctaccaaaa atgaccaagt
    12001 cttaccaaat gagcaggcaa agctgccctg tgacttgccg cccactcaag tactaatcca
    12061 gatacatgcc accattgaca tgaatcgtcg ttccagtgac gtatgcggcg ccaggtgagg
    12121 ccaaaaaacc gacagaagct gcaacgtcct cgggcaagcc aagacgtccg agcggaatgt
    12181 tggcaacgag cgccttccga tgctcctcat ccaggctctt ggtcatgtcg gtatcgatga
    12241 ggccaggtgc gacgcaatta accgtgatgt tgcgactccc caattctcgc gcaagggaac
    12301 gtgtcatgcc ggcgaccccg gccttggcag cacaatagtt ggcttgcccg ggattgcctg
    12361 aatgaccgac gatcgaagtg atattgatga ttcgcccctg acgtactttc atcatgccgc
    12421 gcatgacggc tttggacaaa cggaaaacgg ccttcaggtt ggtgtcgagt accgcatccc
    12481 actcatcgtc tttcatgcgc atcgagagat tgtcccgtgt aatcccggca ttgttgacca
    12541 gaatcgttgt tgcgccataa gttttttcga tgcgtgtcac cagagcatca atctgtgcct
    12601 ggtcacacac ttccagggca gcgccctccc ccgcgatgct tgccgtagcg aggtattccg
    12661 agatgctggc agctccctca ttgctggtgg ccgtgccaat aacggttgca cccagttttc
    12721 ccagttcaag tgcgatagct tgaccgatac cgcgcgaagc gccagtgacg agcgcgactt
    12781 gtccgttaag catttttcct ttcactccat tgacggaatg gctgcttcga tcgccgccag
    12841 atcagccagc gcaaggccgt tcagaccgcc agagcatcgt ttcgtgaggc ccgccaatac
    12901 cttgccaggg ccgcattcgg ccacggtcgt gatgcctatc gatgcgattt tctgtatcgt
    12961 ttcaacccat cgcacgggag agaaagcctg ccgaatcagc gcgtccttta tccttgcagg
    13021 gtctgattcg atggcgacgt caacattgtt gaccacctga atcttcggtt cagcgaaatt
    13081 cagttctgcc agacgagcgc ccaacttgac cgcagccggc ctaatcagcg aggaatggaa
    13141 gggcgcagaa acgggcaaga gcaaggcgcg cttggctcct cgagccttgc atccttcaca
    13201 ggcccgttca acagcggctt tgctaccggc aatgacggtt tggccgggtg cattgaaatt
    13261 gaccctttca acaacctcgc cgttgcccac ttcgtcagcg gcttcagcgc aggcttcttg
    13321 aatcctgtcg tcgtcaaggt tcatgattgc agccatggct cccgttccga cgggcacagc
    13381 ctcctgcatc gcggctgcac gcagacggac aagcggcacc gcatccctga aatcgatgac
    13441 ccctgcggca accagcgccg agtactcgcc gaggctatgc ccagccacga ccgccggaag
    13501 cctgccgcct ttgtcgagcc ataaacgata ggtcgctatg ccggccgtca acatcacggg
    13561 ctgggtgttg accgtttgcg ccagcgcttc ggccggtccc tgcgataaca actcccagag
    13621 atcctgtccg agggttgccg atgcctcatc aaacgttgcg cggaccaccg cagcatcgcc
    13681 gtaggcggcc atcatgccaa ccgattgcga tccctgacca ggaaaaacga aagcgaatgc
    13741 catagataat cctcttagaa ctcaagcaaa accgcgcccc aggtaaagcc gccgcctacg
    13801 ccttcgagca tgactttatg accgcgctga atgcgcccat ctcgaaccgc cacatccagt
    13861 gccagcggaa tggaagcggc ggaggtattt ccatgttggt caaccgtcac aataaatttt
    13921 tccatgccaa gccccagttt cctggcagtc gattcaatga tccggatatt ggcctggtgt
    13981 gggatcaccc agtcgacttc agatggagaa atgccggcgc cttcgcaaca ttccttcgcc
    14041 acatcggtca gcacacggac ggcaaacttg aatacagcct gcccatccat ccgtagaaag
    14101 gggtcgccaa tcacctttcc accgcacaca ctgccgggta ccgacaggat gccatggtgc
    14161 gaaccatcgg cgtgggccgc agtagccaga ataccggggg cttcagacgc ttccagaaca
    14221 accgctcccg cgccatcccc aaatagcacg catgtggtgc gatcttccca gtcaaggatc
    14281 cgtgaaaaga cttcggcgcc aacaaccaaa gccttcttgt ggctacccga acgaatgaac
    14341 ttctcggcaa cagtcagggc ataggcaaat ccactgcaca ccgcctggac atcgaaagcc
    14401 atggcaccct gatttcccag ttttgcctgc aacaggcagg ccgtgcttgg gaagatgaag
    14461 tcaggagtcg aggtcgcaag gattatcaag tcgagctgcc cggcatccgt tcccgctgcg
    14521 gcaagcgcgc gacggccggc ttcgagcgct agatcacttg atgtgcaact cgagtcagcg
    14581 aggtggcgtg tcctgatgcc cgtacggctg aagatccatt cgtcatctgt ttcgaccccc
    14641 cgattgagaa gatcggtgtt gctgactggc tgtccgggaa gataactgcc aactcctgtg
    14701 atgcgggcaa acatgctatg caatctcctg caatggttgt tgcaatgcga cacgttcggt
    14761 gatgcgtgac agcaccccgg ttcttgcttc ttctgccgca cgctcaattg cgttggcaaa
    14821 ggaaaaaacg tcggcagatc cgtggctctt gacgactatc cccttcaggc caagcagagt
    14881 ggcgccattg tatcgcctgt gatcgacatg cttcttgaag ttattcaaga cgggcaaggc
    14941 aatcaaggcg acaagtttgg tgagcagact gcggttgaac tcccgcttca gaaaagtagc
    15001 cagcatctgt gccagacctt ccgaggcctt gagggcgacg ttgccaacga agccatcgca
    15061 aacaataact tcagcctcac ccttgtagat accatccccc tcaacatttc ccacaaaatt
    15121 caggccggac tccctgagca actcggccgc tcgcttaacg acgtcgtttc ctttgatagc
    15181 ttccgcgcca atattgagaa tgccgaccgt tggccgatcc ttgtgttcca tagcggcaac
    15241 cagcgaagcc cccatgatgc caaattgcaa cagatgctcg gggctgcaat cgacgttggc
    15301 tcccagatca agcacgtgcg aatggccttt ggccgtcgga agaatggcgc agatcgccgg
    15361 gcggtcaatc cccggcagca tcttgaggac aaagcgcgaa atggccatca gggcaccagt
    15421 attgccggca gaaacacagg catccgcttg accttgtttg acgaggttaa tggcgacccg
    15481 cattgacgaa tccttcttgt tgcgcaatgc cagcgccggc gcctcatcca tcgctacaat
    15541 ttcgcttgcg tgatgaattg caatacgact atctgctgat ctcgcaagga acgggctgat
    15601 cttctcttcg atacccacga ggatcacgct tacgtcatcg tgattttcca cgaagtctcg
    15661 cgccgcacgc acggtaaccg cagggccaaa atccccgccc atgcagtcaa tagcgacagt
    15721 aaccgtcatc cggaaggagc tcaaagaaaa ggcaggcaag ttcccacgat ggggaattgc
    15781 ctgcccaatc ctgtaacctt actctcctgt accctttgtg actttcttgc cacggtaata
    15841 gccggacggg ctaatgtgat ggcgaagatg gacttcaccg gtcgtcggtt caacggccag
    15901 cggaggattt gtcagaaaat cgtgggcgcg acgcatgcca cgcttggacg gtgatttctt
    15961 gttctgttga acggccataa attactccat agatcaaacg cactatgctt tgcccttgaa
    16021 accttgcagc acggcaaagg gggaaatctt aacttttccc gctttgatat caggcaaagc
    16081 acaacttttg tgacgcggcg ccgagggcag atcaagaatg atttcgtctt caatcaacgc
    16141 aacaacatcg agttcttttt gcgctggaag gaaatccatt gaatcatcct cgatctcctc
    16201 ctgcgtcaat tcttcgtcat cgacgaattc cagcacgctc cgcacctcga ccggataatc
    16261 aatcccctca aggcaacgct gacagcaaac tgacaacaca ccatccaact gcaattgcaa
    16321 ctgcggcctg ttgcgcgcac ccatccggcc ctcgatccga aaactcaaat aaccagctga
    16381 accggcaagc ttatcgagaa cgcgagccaa gtcggcaatc agcaatttgc cttgcaaggc
    16441 acctgcttcc cgcgcaaagg tcagactgtc aataacaatc cgttccgaca taaggggcgc
    16501 atgatattat ttttggcttt cgaagtcaaa gcagtagcgc cattgttctg atgtttattg
    16561 atctttaact ttgtcccgcc acggtggcgg tatttccgtc tctctccaca tgcccagcgt
    16621 acagattgtt ctttcctcga cctccctatt ccgacgtgaa ctgctcgccc ggcttggttt
    16681 gccgtttgaa acggccaacc cgaatgtcga cgaaactgca cggcacggcg agacaccgga
    16741 aacaactgcg ttgcggctat cggaagacaa ggctcgagca gtcgcaaggc agtatccgga
    16801 ggcgctgata attggtagcg atcaggttgc ctatcttgat ggtcaggttt ttggcaaacc
    16861 aggaacccat gagaatgcag caaggcaatt gcagacgatg cgtggccgca ccgtcaactt
    16921 ctacaccgga ttgtgcctgc tcaacgccaa aagcggcaaa gtgcagttgc gtggtgtgcc
    16981 cacgcttgtc acttttcgga acctgacgag cgatgagatc gaaaactact tgcgcaagga
    17041 gaagccctac aactgcgccg gcagtgcgaa atccgaaggc ttgggaattg ccataatcgc
    17101 aaagatggag ggagaggatc caaacgcgct cattggccta cctctgattg cgctctgtga
    17161 ttttctaaga aatgagagtg tttatgccat ataaacatat cgttatagcg tctatttaaa
    17221 gtattttatg gaaaaaggaa ggctatattt agtcccggtt ccactcggcc cagtagcggc
    17281 cgaatccgtc ttgccggcac ccgtgcttga ctgtgcgaga aagcttaagc attttgtggc
    17341 ggagagcgcc aagtcggctc gagccatgct caagtcgctg ccttccgatt acccgttgca
    17401 gttgatcgag attcgcgaac tcaacgaaca tacctcgctc aatgcgttac ccgatttgct
    17461 ggctcctcta ctcgcgggaa tggacgttgg cctgatttcc gaggctggct gcccggctgt
    17521 ggcggaccct ggagccaatc tggtcgcctt ggcgcacagg gagggaatcc aggtcatccc
    17581 gatgatcggc ccatcgtcaa ttctcctcgc cttgatgggg tcgggtctgt ccggccagca
    17641 tttcgcattt catggttatt tgccgacgaa ccaagagcag cggcaaaaga gactccgcga
    17701 actggaaaag gattcgcgtc aagggggaaa aacgcagatt tttatcgaaa cgccttatcg
    17761 caattgccaa atgcttgaaa ccctgatcag tgtctgcgcc caaaacacga aaatctgcgt
    17821 tgccaccgat ctaacgttgg gtagcgagag catcttgacg cgcacgcccg ctgaatggcg
    17881 ccgacagggg gtgccgagca ttaaccgccg cccgacggta tttctgattc aggcctgaaa
    17941 tacccacacc cggaaaaagg aaagccttag tgaagtcctg tccggttgct gccgaacagg
    18001 cgaaatacga tgctttggcc tgtttccgcg cccaaacgtt tggcaaagcg ctccgcgata
    18061 ttctctttga ctgaatagtc gagtatggtt tcagccttga tcaagtctct ggccactgaa
    18121 tcaaccgtcc cgaaatcatc ggcaagcccc atcttgatgc tctgactgcc ggtccacatc
    18181 aatccggaaa acatatcggg cgtctctttg agtcgatttc cacgaccgtt acggacgacc
    18241 tcaataaact gcctatgtat ctcgcccagc agtatctggg catgctcttt ctgtctttcg
    18301 tcctgtggag agaaggggtc gaggaaccct ttgttcactc cggccgtcag caggcgccgt
    18361 tctacgccaa gcttgtccat tgtcccggta aaaccgaatc cgtccatcaa tacgccgatg
    18421 gaaccaacga tactggcctt gtcgacaaaa atcttgtcgg cggcagaggc aacatagtac
    18481 ccaccggaag cacacacatc ctcgactacg acgtaaagcg gaatttgagg atgcttggtc
    18541 cgca
//
LOCUS       DAORPM010000012        18389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_543_length_18389_cov_10.614814, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000012 DAORPM010000000
VERSION     DAORPM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 18389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18389
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_543_length_18389_cov_10.614814"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..656
                     /locus_tag="PLD53_01235"
     CDS             300..656
                     /locus_tag="PLD53_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HRC59557.1"
                     /translation="MLKVYGIKNCDTMKKAFAWLEANAVAYEFIDYKQPDVAQNHLPD
                     WNRRAGWQRLLNTNGMMWKRLSDEERVDVDEAKALALMADYPTLIKRPVADTGSDLLV
                     GFEPERYEKALKRELG"
     gene            653..1519
                     /locus_tag="PLD53_01240"
     CDS             653..1519
                     /locus_tag="PLD53_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRC59558.1"
                     /translation="MTGDCVQRFMLDDLDIRGAVVRLGAVWRQLLTDRDYPAPVVDLL
                     GRMSATSLLLADNLKQPGRLTIQLRGNGPVSLLVIDCNETLNTRCMAQHDEEIDTTAD
                     IDLLGDGQLLLSLDTPARRKPYQSIVPLAGKNIAEIFEHYLMQSEQLASRFFLAATPT
                     GVAGLFLQKMPTTDERDSDGWTRIEALAATAKPKELLELPIGELLTRLFHEETIRLFA
                     ARAVTNNSPEDWEKVGNMLRSLGREEVYGALSERGEILVRDDLGNREYRFDKRAIDAL
                     FSNTPETPPTVH"
     gene            1630..3105
                     /locus_tag="PLD53_01245"
     CDS             1630..3105
                     /locus_tag="PLD53_01245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRC59559.1"
                     /translation="MALALIALLTVVSQVGVQYLIASQDYDSRVVNIAGRQRMLSQRI
                     TKMTYYIAQAESAQSAAIFRLQLAEMLSLWQRSHQGLLRGDVDLGLPGKNSDEVIALF
                     KQIEPDYQAMVTAARHLVEASERGESISQDVQQIREHEQNFLQGMDAIVFHYDEEAKA
                     KISAARWYFLILMTITLIVLALEAVLIFAPVTRRIRHDMRTLEKKEEDMHSLFSGNPT
                     AMLLVNRKDLAIIKANEKACDLLGAAAEEISRNDLRSYLDATSDTNGVFLDAIGKHEP
                     LSSYEVVLLDAQRTEVSALASIREIDFAGHEVYLLGLTNISELKKAQQTLEYYATYDE
                     MTGLLNRRTGLLVLENALARAKRDRRELVVCFLDLDGLKATNDRFGHAEGDWMLRTTA
                     KALTDSIRSGDVAVRLGGDEFLMIFHDCPVAEATRLLARIEEKLGAVETRELKPFPIS
                     VSYGLAAYDPVLHSTPDDLIAEADALMYIAKQKRKQPAAGA"
     gene            complement(3161..4333)
                     /locus_tag="PLD53_01250"
     CDS             complement(3161..4333)
                     /locus_tag="PLD53_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC59560.1"
                     /translation="MNTRSLLPVIQWIEGGEGRSAVWRSEAGVSPPQRVHVADDTATA
                     DAAFRLACEGTALLWRGDFQNARQLLQAMARRFDRKPRRRRNPGALSHSPSEAFHQQR
                     MAQAQRARTLGMLLIPVEKDHHILQRRAPDVQQACEEAYGAADAPYVVSLRELLGLIG
                     AHQWRKNGVEIPALGGRIHPHYGVFSPVRGEYVALVADAPLPELLIHSPPSNSLAFDI
                     GTGTGVLAAVLASRGVARVIATDQDQRALDCASENLTRLGFGKQVEVQLADLFPPGRA
                     PLIVCNPPWVPARPSSPLEYAIYDPGSRMLRGFLDGLAAHLEPGGEGWLILSDLAEHL
                     QLRSHSELLALFEQGRLRVLGRHDVRPAHPRATDSADPLHAARAAEVTSLWRLGAT"
     gene            complement(4425..5324)
                     /locus_tag="PLD53_01255"
     CDS             complement(4425..5324)
                     /locus_tag="PLD53_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC59561.1"
                     /translation="MNNHTDVAKTTEISGVAFRQRSVCCASPTGLHHMAYNEWGDHDN
                     PKVLVCVHGLTRNARDFDQLARVLAGNYRVICPDIVGRGRSDWLRDPTGYTIPQYLHD
                     MLVLIARLDVETVHWVGTSMGGLIGMALASLENTPITRLLLNDIGPVIPSESIKRIGD
                     YVGRAPSFDTYDAAENYIRIVSAPFGALNDDQWRQLTESSLRQNADGRLEMRYDPGIG
                     ESFRREMSGGDIDLWPVFDRVGCPTLVVRGAESDLLKAETLQAMALRGPHPAVVEVPG
                     VGHAPMFLDEAQISIVRDFLLAA"
     gene            complement(5340..7706)
                     /locus_tag="PLD53_01260"
     CDS             complement(5340..7706)
                     /locus_tag="PLD53_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRC59562.1"
                     /translation="MRSIIVAFACGVGLLQLQGNLPGADVIGALAALALLGLFLVVRR
                     EHWAFRLLALFCCVLLGFVWAGWRAQQRLADQLPIAWEVKDIQVTGVIAALPQRFERG
                     ERFEFDVESVHTKGGVVPGRIMLSWYHAWDELDGSREGIEVSAASEAGEAREARAIHP
                     GERWRFTVRLKRPHGTANPHGFDYEAWLLERNIRATGTIRLRGEARRLDAFVPSPGYA
                     VERLRDIIRSRFLAAMPDAPYLGILIALTVGDQRSIPNEQWTVFNRTGVTHLMSISGL
                     HVTMVAALFAALVNWLWRRSERLTLYLPAQKAVVVAGWLAAFAYSLLAGFEVPAQRTL
                     YMLSVVALALWSGRNLGVSRTLLLALLVVLLVDPWAVLATGFWLSFGAVALLLLVGTA
                     RIGEVRGWNATLARWGVAQWAVTLGSMPLLLLFFHQFSLVSPLANALAIPFVSFIITP
                     LALSFAVLPWMPLLHLDHWLLSHLMVWLEWLADWPMWQQAALPLWVTLVAVAGIVWLL
                     LPRGFPARWLGLCLLMPALFWPPPRPGIGEAWVDVLDVGQGLAVVVRTAGHTLLYDTG
                     PLYSANSNAGQRIVLPYLRASGVGQLDTLIVSHRDKDHAGGVAAVREAVPIARILTSM
                     SMLGGEPCIAGQSWEWDGVHFFILHPVSTDYQINSRKTNNMSCVLRIASAEGSVLLTA
                     DIEADDERILIARSPSLLPSDVLLAPHHGSSNASTPEFIAAVGARDVIFSAGYRNPFK
                     HPRPEVLERYSTNRQWRTDRDGAVRIVLGESTEVSAWRKERQRYWQLQ"
     gene            complement(7778..9412)
                     /locus_tag="PLD53_01265"
     CDS             complement(7778..9412)
                     /locus_tag="PLD53_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRC59563.1"
                     /translation="MSIQKDVATKSERGMLLLALSILVLLTVVAIVSRPLTPIDETRY
                     ISVAWEMWLRGDLLVPFKNGAPYSHKPPLMMWLYQAGWAVFGVNEWWPRLVHPLVSAG
                     NLLLMIGLSKRLWPDRPDIGGPAALILVSSLLWTIFSTSAMFDVILAFFTLLGMYGTL
                     VAADGERRRGMVWLGVAIGLGVLAKGPVILLHVLPVAVLAPWWKPGLRRAHWYGGIFL
                     AVLLGAGIALLWAIPAGMSGGEEYRKAIFWGQTADRMVESFAHRRPMWWYLPLLPVIF
                     FPWFIWPGLWRTLRRFIGRDIDRGGRFCLAWMLPVFVAFSLISGKQPHYLIPIFPAFA
                     LLAARSLVAEERIKGLWMPTLAALSIGVAFLVMAKGGLGPKVGDQKMFTEMWPGLLMI
                     TAVVLLYLFGRHTANPIPALALLGAGVSALGQLALARPLYPAYDVRPIAIAIHDAQAN
                     GHLVAHLGEYHNQYHFAGRLQSPLPLFDDAEPLKAWLIDHPEAYVVLYPKSTRSLAGI
                     EVIATQSYLGGAVALLAAQPALSVLSTQESIDSRLD"
     gene            complement(9519..10223)
                     /gene="lolD"
                     /locus_tag="PLD53_01270"
     CDS             complement(9519..10223)
                     /gene="lolD"
                     /locus_tag="PLD53_01270"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HRC59564.1"
                     /translation="MNEATRDSGKVVLACQGLRKIYRQGTGEGSEVPVLMGVDLEIVA
                     GERVAVVGASGSGKSTLLHLLGGLDSASEGKIQLMGRDLSEISDAERGNMRNRHLGFV
                     YQFHHLLPEFTALENVAMPLYIRRLPKPDAEERAKAVLDEVGLSHRLDHTPSELSGGE
                     RQRAAIARALVTEPACVLADEPTGNLDRQTAESVFELMLALNRSRHTSFIIVTHDPGL
                     AGRTDRILTLRDGSFL"
     gene            complement(10216..11463)
                     /locus_tag="PLD53_01275"
     CDS             complement(10216..11463)
                     /locus_tag="PLD53_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRC59565.1"
                     /translation="MPLPYELLIGLRYTRAKRRNHFISFISMISMLGIALGVAALIVV
                     LSVMNGFQTELRGRILAVVSHVQISGANGEMTEWQRVAEQSGRQPGVLAAAPFVQAQG
                     MLSFGQSVRGAMVRGILPDLEDKVVDFKTHMKEGSLDALVPDSFNIVLGSELARALGV
                     FVGDKVTVIAPQGVVTPAGVIPRLKTFTVAGLFEVGMFEYDSGLALIRMEDAQRLYRM
                     EDRVSGVRLKLDDLFKAPLITRQLANSLDTAAYISDWTRSHANFFRAVQIEKNMMFII
                     LSLIVAVAAFNIVSTLVMAVTDKQADIAILRTLGANPGSIMAVFMVQGALIGFIGLGL
                     GVAGGVTLALNVDVVVPFIERLLGTQFMSKEVYYISNLPSELQWKDVTTITGVSFVLS
                     LVATIYPSWRASRVNPAEALRYE"
     gene            11902..>13742
                     /locus_tag="PLD53_01280"
     CDS             11902..>13742
                     /locus_tag="PLD53_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HRC59566.1"
                     /translation="MAAQAAGLGKIVVLSSLGQPLRAEIDVLATRDEFSGMKAQLAPP
                     DAFKQAGLDYATTLLSIRFKLDKRADGQPVLRLTSDRPINDPFVDMLLELNWPAGRLV
                     REYTFLLDPPEVAAKGFPPLAPAMAKPATASKQAVATTRTKSAIDDELRSKAVARIRN
                     PGSAQKSAAQPSSSGPDTLEVKRGDTLHKIAGESKPDGVSLDQMLVGLFRANQDAFDG
                     GNMNRLRAGKILVVPEKSTVEAVSSGEASKIVRAQASDWNAYRNKLAGVAAQAPVSDE
                     AARQEATGKISAKVEDKAAPSVEPKDQLKVSRTETTAAKPDVGAKRSEEDLIAKEKAL
                     KEANERLIALEKNVSDLQKLVELKNQNLAELQAQASSKAMPLESIKPTEETKPPATTP
                     DPVPVAPPAKIEPVATAIEKPAEKPAETTPPEPAPKPEEPKPEPKPAVKPKPAIAPPP
                     PLEEPGFIDGLLDNPLALAGGGGVLALLAAYFVSRRRRAGKEEVPLDSSSTLSPQSSS
                     LSLTANSVFRNTGGQSVDTSHTPAQTDFSQAGPGSIDTDEVDPVAEADVYMAYGRDVQ
                     AEEILVEAKQKDPKRHAIHLKLLEIYSNRKDLKQFETLATDLYGDTGG"
     assembly_gap    13743..13842
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13843..14835
                     /locus_tag="PLD53_01285"
     CDS             <13843..14835
                     /locus_tag="PLD53_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HRC59567.1"
                     /translation="ELEETHRLPEPVIDSSALDFDLAADTGTSDSATDADATVVGLDF
                     DLPDAAKAEASDDGTVATAPDLDFDLGFEPSPAETAEPKTDSPDSSNAAASAVSSDDV
                     DFDISMTESTFLGRPMPEPSSFDMSSIDLDLKEPEAEASASTPPPSVAEPDSSSDLPD
                     SSFDDVQVSTSVNPDFETEQSVTIVNPQFGTDQDDLMPEQDFSSEQMATAVNPQFDGE
                     QTLVAGRDFSATQEDTVVNPQFDGEQTLVSGRDFSATQSETVVNPQFGDDQDLAPEFE
                     ISPNEEVTTKLDLAKAYEEMGDLEGARELLQEVLKEGDAAQQEKAQAILAKISE"
     gene            15017..15619
                     /locus_tag="PLD53_01290"
     CDS             15017..15619
                     /locus_tag="PLD53_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HRC59568.1"
                     /translation="MPKLTIHPTTCLIVWLLFLVVVQFMSGTTLVVTLLLLPALGTYV
                     LQRGSRLIWRARWLLISMLAIFSWGIPGDPLWNGAFAPTHEGLREGLTHFGRLLLVLV
                     AVAAFLEAMPLPELLAAMHTLLGPLRHLGLDPDRGVVRLMLVLRYVEALPRPRDWRTL
                     IDAPATNLGEAVEVNHRPLRWPDYFVALALALAAAFYVFR"
     gene            15629..16420
                     /gene="truA"
                     /locus_tag="PLD53_01295"
     CDS             15629..16420
                     /gene="truA"
                     /locus_tag="PLD53_01295"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRC59569.1"
                     /translation="MRIALGVEYDGSAFHGWQKQPDGNTVQDALQSALSGIAGVPIQV
                     VAAGRTDTGVHATGQIVHFDTWIERPLNAWVRGTNTYLPAAVAVRWAHPVSDSFHARF
                     SAYGRRYRYIVINRPQRTGVWQRHAGWYHHPLDVGRMRQAARMLVGEFDFSAFRAADC
                     QAKSPVKTMRQADVQRYGDTIVFDFEAGAFLHHMVRNLVGSLVYIGQGKHPPEWMQQL
                     LASGDRRIAAPTFSAAGLYLVGVKYEPHWGLPNSDDFFLPGMAGL"
     gene            16455..17090
                     /locus_tag="PLD53_01300"
     CDS             16455..17090
                     /locus_tag="PLD53_01300"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRC59570.1"
                     /translation="MEKPRIKICGLTRPEDVQAAVDAGADAIGLVFYPPSPRYVALET
                     AAELARTVPPFVTIVGLFVNAAPEVVKETLAAVPIHMLQFHGDEDEVYCRQFARPYMK
                     AARVKPGLDLVQYVVGFPSAQAILLDAFVEAYGGGGQVFDWTLIPPALGKPIVLSGGL
                     DARNVGDAIIRVRPAAVDVSSGVEATKGIKDADKIRAFVTAVRAAGLMPTD"
     gene            17260..>18389
                     /gene="trpB"
                     /locus_tag="PLD53_01305"
     CDS             17260..>18389
                     /gene="trpB"
                     /locus_tag="PLD53_01305"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRC59571.1"
                     /translation="MVAPRYDLPDSLGHFGPYGGVFVAETLIPALEELKEAYANAQRD
                     PLFIAEFDYDLKHYVGRPSPIYHAKRWSELLGGAQIHLKREDLNHTGAHKINNCIGQA
                     ILARRMGKPRVIAETGAGQHGVATATVAARYGMECVVYMGSEDIKRQAANVYRMKLLG
                     ATVVPVDSGSRTLKDALNEAMRDWVTNISNTFYIIGTVAGPHPYPMMVRDFQSIIGRE
                     AIGQMQEQHGRQPDAVIACVGGGSNAMGIFYPYIPVEGVRLIGVEAAGDGIETGHHAA
                     SLTAGRPGVLHGNRTYLLQDENGQIIETHSISAGLDYPGVGPEHAWLKDSGRAEYVTI
                     TDAEALQAFHDLCRQEGIIPALESSHALAYAARLAPSLPKDR"
BASE COUNT         4092 a         5517 c         5169 g         3511 t
ORIGIN      
        1 catggggtgt gaattcggac aggggtatct ttactcgcca gcgatgccgc ccgaagccct
       61 ccccggctgg ctggacggcc gaaatgccgg taagcgtgaa catcccggct agctccggtt
      121 cgttgtcgtt tgcggatttc cgcttggggt gttgccggac cgcggcctgc tcgccgatcc
      181 aacattgacc tttggcgtgt ggcggagcgc ttgagttttc cgccacgatt gcgcatagtt
      241 cgcctttttt caagccagcg ccttcgccaa actcaggcga tgaaacagga aaacgggaca
      301 tgctcaaggt ttacggcatc aagaactgcg atacgatgaa aaaggctttc gcctggctgg
      361 aagccaatgc cgtcgcttat gaattcatcg actacaagca gcccgacgtc gcgcaaaacc
      421 acctgccgga ctggaaccgg cgcgccggct ggcagcgttt gctcaacacg aacggcatga
      481 tgtggaagcg gctgagcgac gaagagcgtg tcgatgttga tgaagccaag gcgctggcgc
      541 tgatggccga ctacccgacg ctgatcaagc gcccggtggc ggataccggt agcgacctgc
      601 tcgtcggctt cgagcctgag cgttacgaaa aagcattgaa gcgggaactt ggatgacagg
      661 agactgcgtt cagcgcttca tgcttgatga cctcgatatc cgcggcgccg tcgtacgcct
      721 tggcgccgtc tggcggcagc ttcttaccga tcgcgattat cctgcgccgg tggttgatct
      781 gctcgggcgg atgagcgcca cctccctgct cttggcagac aacctcaagc agccggggcg
      841 actcaccatc cagttgcgcg gcaatggccc cgtatcgctg ctggtgatcg actgcaatga
      901 aacgctcaac acccgctgca tggcgcagca cgacgaagaa atcgatacga ctgcggatat
      961 cgaccttctg ggtgacggtc agcttttgct ctcgctcgac acgccagcca ggcgcaaacc
     1021 ctatcaaagc attgtgccgc tcgccggcaa aaacatcgcc gaaatcttcg aacactatct
     1081 catgcagtcc gaacagctcg cttcgcgttt tttccttgcc gcaacgccga ccggcgtagc
     1141 cggcctgttc ctgcagaaga tgccaaccac ggacgaacgc gatagcgacg gctggacgcg
     1201 tatcgaagcg cttgcggcca cggccaaacc caaagaacta ctcgagctgc cgatcggtga
     1261 acttctcaca cggctgttcc atgaagaaac catacgtctg ttcgccgcgc gggcggttac
     1321 caacaactct cccgaagact gggaaaaggt gggcaacatg cttcgctcgc tcggccgcga
     1381 ggaggtctat ggcgcgctca gtgaacgcgg cgagattctc gttcgcgacg atcttggcaa
     1441 ccgcgaatac cgcttcgaca aacgcgccat cgacgcgctc ttcagcaata cgccggaaac
     1501 accaccgacg gttcattgaa cgcagtgccc cggcactgaa ttgtcgagta tcatcgcgcc
     1561 atgtcagatt cacggcttat ttcaagcgcc tcctcgatca cggtgggccg gctccgcaaa
     1621 cgctgtatct tggcgctcgc gctgatcgca ttgctgaccg tcgtttcgca ggtgggggtt
     1681 cagtacctga ttgccagtca ggactacgac tcgcgggtgg tgaatatcgc aggccggcaa
     1741 cgcatgctga gtcagcggat caccaagatg acctactaca tcgcccaggc ggaatctgcc
     1801 cagtcagccg caatcttccg tctgcagctg gccgaaatgc tgagcctttg gcagcgctca
     1861 catcaaggac tgctgcgcgg cgatgttgac ctcggtttgc ctggaaagaa cagtgacgaa
     1921 gtcatcgctc tattcaaaca gatcgaaccc gattatcagg ccatggtcac tgcggcaaga
     1981 cacctggtcg aagcgtccga acgcggcgaa tcgatcagtc aggacgtcca gcagatcagg
     2041 gaacacgagc agaatttctt gcaaggcatg gacgctattg tcttccatta cgatgaagag
     2101 gcaaaggcca agattagcgc cgcacgctgg tattttctga tcttgatgac catcacgctg
     2161 atcgtgctgg cactcgaggc ggtcctcatc tttgccccgg tgacgcgacg cattcgccat
     2221 gacatgagga cgcttgagaa gaaggaagaa gacatgcaca gtctcttctc cggcaacccg
     2281 accgcaatgc tgttggtcaa caggaaggat ttagcgatca ttaaggccaa cgagaaagct
     2341 tgcgacctgc tcggagcggc tgcagaggaa atttcgagga acgacctgcg cagctatctc
     2401 gatgccacca gtgataccaa cggcgtattt cttgacgcca tcggcaagca cgagccacta
     2461 agcagctacg aggtcgtcct gctcgatgcg cagcgcactg aagtcagcgc cctggcctcc
     2521 attcgcgaga tcgatttcgc cgggcacgag gtctacctcc tcggcctgac caatatcagt
     2581 gaactcaaga aggcacaaca gaccctcgag tactatgcga cgtacgacga aatgaccgga
     2641 ctgctgaacc ggcgtaccgg tcttctggtg ctcgaaaacg ccctggcgcg cgccaaacgc
     2701 gaccgcagag aacttgtcgt ctgctttttg gaccttgatg gcctgaaagc aaccaatgat
     2761 cgttttggcc acgccgaggg ggactggatg ctgcgcacaa cggccaaggc actcaccgat
     2821 tcaatccgct ctggcgatgt cgccgtgcga ttgggcggag acgagttcct catgatcttc
     2881 cacgactgtc cggtggcaga agctacgcgc ctgctcgcac gcatcgagga aaagctaggc
     2941 gctgtcgaga ccagggaact caagccgttt ccgatcagcg tcagctatgg cctggcagcc
     3001 tacgaccccg tacttcactc aacgcccgat gatctgattg ccgaggccga tgcgctgatg
     3061 tacatcgcca aacagaagcg aaagcaaccg gctgcaggcg cttgaatccg cccaccccgg
     3121 tttgctgcct cttcgttctg cccccctact acctatccga tcacgttgcc ccgagtcgcc
     3181 atagcgaagt aacttcagca gcgcgcgccg cgtgcagcgg atcggccgaa tcggtcgcac
     3241 gcggatgggc cggacgaaca tcatgccggc ccagcacacg tagtcgccct tgctcgaaga
     3301 gggccagtaa ctcgctatgc gagcgcaatt gcaaatgctc ggccagatcg gaaaggatca
     3361 gccagccctc gccacccggt tccaggtgtg cggccagccc gtcgagaaat ccccttaaca
     3421 tacggctgcc cggatcatat atggcgtatt cgagcggaga gctcggccgc gccggcaccc
     3481 agggtggatt gcagacgatc agcggtgcac gtccgggcgg gaagagatcg gccagttgca
     3541 cctcaacttg ctttccaaaa ccaagccgcg tgagattctc acttgcgcag tccagcgcac
     3601 gttgatcctg atcggtggcg atgacgcggg ccacgcctcg gctggccaaa acggcggcaa
     3661 gcaccccggt tccggttccg atatcgaagg ccagactgtt gcttggtggc gaatggatca
     3721 acaattcggg caagggcgcg tcggcgacta gtgccacgta ctcgccacgc accggtgaaa
     3781 acacaccgta atgcgggtga atgcgtccac caagcgctgg tatctcgact ccattcttgc
     3841 gccattggtg ggccccgatc aggccaagaa gttcgcgcag cgacacaaca tagggcgcgt
     3901 cggccgcgcc ataggcttct tcgcaagctt gctgaacgtc gggtgcgcgg cgctggagaa
     3961 tatggtgatc tttctcgaca ggaatcagca gcatgcccag tgttcgggca cgctgcgcct
     4021 gagccatacg ctgctgatgg aaggcttcac tgggcgaatg cgacaaagcg cctggattgc
     4081 gtcgcctgcg cggtttgcga tcgaagcgcc gcgccatggc ttgcagcaac tgccgggcat
     4141 tctgaaaatc gccgcgccaa agcagcgcag tgccctcgca agccaggcga aaggccgcat
     4201 cggcagtcgc cgtatcgtcg gccacatgca cgcgttgggg tggagaaaca cctgcttcgg
     4261 agcgccagac cgctgaacga ccttcgcctc cttcgatcca ctggataacc ggcagcaagc
     4321 ttctagtgtt cacgtggtcc catcgatgaa tctgccggcg cgtgattcag ccgcatcggc
     4381 atcggcatcc agcaagtcac tcattcagag tcgccagatg atcgtcaagc cgccaagaga
     4441 aaatcccgca cgatactgat ctgtgcctca tccagaaaca tcggtgcatg accgacgccc
     4501 ggcacttcga caacggccgg atgcggcccg cgcaatgcca tggcttgcaa ggtctcggcc
     4561 ttgagcaaat cggactcggc accacgcaca accagcgtcg ggcaaccgac acggtcaaaa
     4621 acaggccaca gatcgatgtc tccaccgctc atttcacggc gaaaggattc gccgataccg
     4681 gggtcataac gcatttccag gcgcccgtct gcgttttggc gcaggctgga ttcggtaagt
     4741 tgccgccatt gatcgtcgtt gagcgcgcca aaaggggcac tgacaatccg gatgtaattt
     4801 tcggcggcat catacgtgtc aaaacttggc gctcggccca cgtagtcgcc gatgcgcttg
     4861 atcgactcgg acggaatcac cggcccgata tcgttcagga gcaaccgcgt aatcggtgta
     4921 ttttcgaggc tggccaaagc catgccgatc agcccgccca tcgacgtgcc gacccaatgc
     4981 accgtttcca cgtcaagacg cgcaatcagc accaacatgt catgcaaata ctgcggaatg
     5041 gtgtaacccg tcggatcacg caaccagtcg ctgcgtccgc gtccgacgat gtccgggcag
     5101 atcacacgat aattgccggc caacacgcgt gccagttggt caaaatcgcg tgcattgcgc
     5161 gttagaccat gtacgcaaac gagtactttc ggattgtcat ggtcgcccca ttcgttgtaa
     5221 gccatgtggt gtaaaccggt cggcgaggcg cagcagacgc tgcgctggcg gaacgcaacg
     5281 ccagaaatct cagtcgtttt ggcaacgtca gtatggttgt tcatataccc agtctagcat
     5341 cactgcaatt gccaataccg ttgtcgctcc ttccgccatg ccgaaacctc tgttgattcg
     5401 ccgagaacga ttcgaaccgc cccgtcgcgg tcggttcgcc attgacggtt ggtcgagtag
     5461 cgttcgagca cttccgggcg cgggtgtttg aaaggattac ggtagcccgc ggaaaaaatc
     5521 acgtcgcggg caccaacggc cgcgatgaat tccggcgtcg atgcgttgct gctgccatga
     5581 tgtggtgcca gcagcacgtc acttggcaac agggatggcg agcgggcgat caggatcctc
     5641 tcatcgtcag cctcgatatc ggcagtgagc aggacgctgc cttcagcgct cgcaatgcgc
     5701 agcacgcagc tcatgttgtt ggtctttctt gaattgatct ggtaatcggt cgacaccgga
     5761 tgcaggatga agaagtgcac tccgtcccat tcccaactct gccctgcgat acacggctct
     5821 ccccccagca tggacatcga agtcagaatg cgagcgatcg gcacggcctc gcgcaccgct
     5881 gccacgcccc ccgcgtgatc cttgtcgcga tgcgagacaa tcagtgtgtc aagctgacca
     5941 actccgctcg cgcgcaaata cggcagtacg atgcgttgcc cggcgttcga attggcactg
     6001 taaagcggac cggtatcgta gagcagcgtg tgcccggccg tccgcacaac gacggccagc
     6061 ccctgcccga catcgagcac atcgacccag gcttcgccaa tacccggacg tgggggcggc
     6121 cagaacagcg ccggcatgag caggcacagc ccaagccagc gtgccgggaa gccgcgcggc
     6181 aacagcaacc agacgatacc cgcaacggcg acgagagtaa cccacagcgg cagtgctgcc
     6241 tgctgccaca tcggccagtc cgccagccat tcaagccaga ccatcaagtg cgacaacagc
     6301 caatggtcga gatgcagaag gggcatccac ggcagcacgg cgaagctcag cgccaagggc
     6361 gtgatgatga agcttacgaa cgggattgcc agcgcattgg ccagcggtga gaccagtgaa
     6421 aactggtgaa agaagagcag cagcaagggc atgctaccaa gcgtcaccgc ccattgcgcc
     6481 acaccccaac gtgccagtgt tgcgttccac ccccgaacct cgccgatacg cgcagtaccg
     6541 acgagcaaga gcaaggccac cgcgccaaag gacaaccaga agccggtggc caagaccgcc
     6601 cacggatcga cgagcaatac gaccagcaga gccaggagca acgtgcggct gacaccaaga
     6661 ttgcgccccg accacagagc cagggcgacg acgctcaaca tgtacagcgt gcgctgggcc
     6721 ggcacttcga acccggccag cagcgagtag gcaaaagccg ccagccaacc cgccacaacg
     6781 actgccttct gtgccggcaa atacagcgtc aagcgctcgc tgcgtcgcca caaccaattg
     6841 accagcgccg caaacagtgc ggcgaccatc gtcacatgca gaccggagat actcatcagg
     6901 tgagtgactc cggttcggtt gaagaccgtc cattgctcgt tggggatcga tcgttgatcc
     6961 ccgaccgtca gcgcaatcaa tatgccgaga taaggcgcat caggcattgc ggccagaaat
     7021 cggctgcgga tgatatcgcg caggcgctcc accgcgtaac ccggactcgg aacgaaagcg
     7081 tccagccgcc gggcctcacc acgcaagcgt atggttccgg tcgcccggat gtttcgctca
     7141 agcagccagg cttcgtaatc gaaaccgtgt ggattggcag tgccatgggg ccgcttgagt
     7201 cgcactgtaa agcgccagcg ctcgccggga tgtatcgctc gcgcctcacg tgcttcacct
     7261 gcctcactcg ccgcactcac ctcaattccc tcgcgcgaac cgtcaagctc gtcccaggcg
     7321 tgataccagg acagcatgat ccggccagga acgactcctc cctttgtgtg aaccgactcg
     7381 acatcaaatt cgaaacgctc accgcgctca aaacgctgcg gcaaggcagc gataactccc
     7441 gtaacctgta tgtccttcac ctcccacgct atgggaagct gatcggccag tcgctgctgc
     7501 gcccgccacc cggcccagac gaaacccagc aggacgcagc agaaaagcgc caacaggcgg
     7561 aacgcccaat gctctcgccg gacaaccagg aaaaggccaa gcagcgccag cgctgcaaga
     7621 gcgccgatga cgtccgctcc cggcaaatta ccctgcaact ggagcagacc gacaccacag
     7681 gcaaaggcaa cgatgatcga gcgcatgggc ggattagacc tgaaaacggg tctgcaagcc
     7741 cgttaccggg cgctcgttgc cttccgctga atcctggtca atccagtcgg gaatcaattg
     7801 attcttgagt cgacagtaca gacagtgcgg gttgcgctgc cagcagtgct accgcgcctc
     7861 ccagatagct ctgagtggct atcacctcga ttcctgcaag acttcgggta ctcttgggat
     7921 agagtacgac gtaggcttca ggatgatcga tcaaccaggc tttgaggggt tcggcatcat
     7981 caaacaaagg tagcggactc tgcaaacgtc cggcgaaatg gtactgattg tgatattccc
     8041 caagatgggc cacaaggtgt ccattggcct gagcgtcgtg gatagcaatg gcaatcggtc
     8101 taacatcgta agcgggatag agaggccgag ccaatgccag ctgcccaaga gcagacacgc
     8161 cagcgcccag cagagcaagc gcaggaattg gattggcagt atggcggccg aacagataga
     8221 gcaacacaac cgcagtgatc atcagcaggc caggccacat ctcggtgaac attttctggt
     8281 cacccacctt tggcccaagc ccgcctttcg ccatgaccag aaatgccact ccgatcgaca
     8341 gcgcagcgag ggtcggcatc cataaaccct tgatgcgttc ctcagcgacc aaagaccttg
     8401 cggcaagcaa tgcaaacgcc ggaaaaatcg ggatcaggta gtgcggttgc ttaccgctga
     8461 tcaacgaaaa cgcaacaaaa acagggagca tccaagcgag gcagaatcga ccaccgcgat
     8521 caatatcccg cccgatgaat ctcctcaggg tgcgccacaa gcccggccag ataaaccatg
     8581 ggaaaaagat cacaggcaac agtggcaagt accaccacat ggggcgacga tgcgcaaacg
     8641 actcgaccat gcggtcggcg gtctgccccc agaatatcgc cttgcggtat tcctcgccac
     8701 cactcatacc cgcaggaatc gcccataaca gtgcgattcc tgcaccgagc agtaccgcga
     8761 gaaaaatgcc gccataccaa tgcgcgcggc gcaggcccgg cttccaccag ggcgccaata
     8821 ccgcgaccgg caatacatgc agcagtatca ccggcccctt ggccaacaca cccaaaccga
     8881 tggctactcc cagccagacc atcccgcgtc tgcgctcccc atctgcggca accagtgtgc
     8941 cgtacatacc caatagcgtg aagaacgcca ggatgacgtc gaacatggcc gatgtggaaa
     9001 agatcgtcca cagcagactg ctgaccagaa tcagcgctgc cggcccacca atatcaggcc
     9061 ggtcgggcca aaggcgcttg gacaggccga tcatcaatag caggttgccg gccgacacca
     9121 atgggtgaac caggcgcggc caccattcgt tcacaccaaa gaccgcccag ccggcctgat
     9181 acaaccacat catcaacggc ggcttgtgac tgtaaggcgc accgttcttg aatggcacaa
     9241 gcaaatctcc gcgcagccac atctcccaag ccacgctgat ataacgcgtc tcatcaatcg
     9301 gcgtaagtgg ccgcgaaacg atggccacca cagtcagcag tacgagaatg gacaaagcca
     9361 gcagcagcat tccgcgctct gacttggtcg caacatcttt ctggatactc atggcagctc
     9421 taattcgtct gggcacgccg atttggccat ctcactgact tcctttgaaa tcgactcccg
     9481 ataacgagga gtttaaggtt tgatcgctta accagcgctt aaagaaacga cccgtcgcgc
     9541 agcgtcagaa tccggtccgt cctcccggcc agcccagggt cgtgcgtaac gatgatgaag
     9601 ctcgtgtgcc gcgagcgatt gagcgccagc atcaactcga atacgctttc tgcggtctgc
     9661 ctgtcgaggt tgccggtcgg ctcgtcggcg agtacgcagg ccggctcagt gaccaaagcg
     9721 cgcgcaatcg ccgctcgctg ccgctcgccg ccggaaagtt ccgacggcgt gtgatcgagc
     9781 cggtgcgaca aaccgacttc gtcaagcact gctttcgcgc gttcctcggc gtctggtttt
     9841 ggcaggcgcc ggatatataa gggcatggcg acattttcca gcgccgtgaa ttcgggcagc
     9901 agatggtgga actgatagac gaatccaaga tggcggttgc gcatgtttcc acgctcggcg
     9961 tcgctgatct ctgaaaggtc gcgccccatc aactggattt tcccttcgct agcggaatca
    10021 agtccgccaa gcagatgcag cagcgtactc ttgcccgagc cggaggcgcc gactacggcg
    10081 acccgctctc ccgccacgat ctcgagatcg acacccataa ggaccggcac ctcgctgcct
    10141 tcacccgtgc cctggcggta aatcttcctc agcccctggc aggcgagaac gaccttcccg
    10201 gaatccctcg tggcctcatt catagcgcaa agcctccgcc ggattgacgc gtgaagcgcg
    10261 ccagctggga taaatcgtcg ccaccagcga caacacgaag gacaccccgg tgattgtggt
    10321 cacatccttc cactgcaact cggacggcag gttggaaata tagtagacct ctttggacat
    10381 gaactgcgta ccaagcaggc gttcgatgaa aggcacgacc acatcgacat tgagcgccag
    10441 cgtcacaccg ccggccacgc ccaggccaag tccgataaaa ccgatcagcg caccctgaac
    10501 catgaagacg gccatgatac tgcccgggtt tgcgcccagt gttcgcagga tggcgatatc
    10561 cgcctgcttg tcggtgactg ccatgaccag cgtcgaaact atattgaacg ccgccacggc
    10621 cacgatcaac gaaagaatga tgaacatcat gtttttctcg atctgtacgg cgcgaaagaa
    10681 gttggcatga ctgcgcgtcc agtcgctgat gtaggcggcg gtgtccagac tgttggccag
    10741 ttgccgcgta atgagcggtg ccttgaacag atcgtccagt ttcagacgta cgccggaaac
    10801 acggtcttcc atacggtaga gccgttgcgc gtcctccatg cggatcaacg caagcccgct
    10861 gtcatactcg aacatcccga cctcgaaaag gccggcgacg gtaaacgttt tcagcctggg
    10921 aatcacgccg gccggtgtta caacaccctg cggggcgatg accgtaacct tgtccccgac
    10981 gaaaacgccg agcgcccgcg ccagttcgct acccaagacg atgttgaagg agtcaggcac
    11041 cagtgcgtcg agactgcctt ccttcatgtg cgtcttgaag tcgacaacct tgtcttcgag
    11101 atcgggcaga attccgcgaa ccatcgcacc tcgtaccgat tgcccaaagg aaagcatgcc
    11161 ttgagcctgt acaaatggag ctgccgcgag cactcctggc tgtctgcccg actgttcggc
    11221 aacgcgttgc cattcggtca tttcgccgtt tgccccgctg atctgcacat gtgaaaccac
    11281 ggccagaatc cgcccgcgca gctcggtctg aaagccgttc attacggaca ggacaacaat
    11341 caacgccgcg acacccaagg cgatgcccaa catcgagatc atcgaaatga aggatatgaa
    11401 gtgattgcgt cgcttggcgc gcgtataacg cagaccgatc agcagttcgt aaggtagcgg
    11461 catcggaggc aatgattcat gaatgggccg ttggaatgca gcaccaaaat ttggaagtcg
    11521 ttatggtaca cgtaccgcca gagtcgttcg tggcaattta cactgcacgg gctcgcacac
    11581 ttcagcgtgc ccgtatgttt ccgggcgacc gcaaatggaa tctgaaaact ctcacaggtg
    11641 ccagcagtta gggccctcgg cactatttgt aataaatggc cagaaggcgc tcttttcatc
    11701 aaattcgacg gataacaaat atgtcgcgta tcacaagcaa gtttagcttg catcgctaac
    11761 tccatgatgt tattttaata tttccgttcg catgctcagg gtggcgctgt ggctaaaaaa
    11821 attcatcaga cgctaaaaaa tatcggtttg cgaagctcgg catatgccct tgcttcgtgt
    11881 ctcatactct ccggaatacc tttggccgct caggctgcgg gtcttgggaa aatcgtcgtc
    11941 ctttcgtcac ttgggcaacc attgcgtgcg gagatcgacg tcttggcgac gcgcgatgag
    12001 ttctccggaa tgaaggcgca gttggcgccg ccggatgcct tcaaacaggc aggactggat
    12061 tacgcgacaa ccctgctgag catccggttc aagctggaca agcgagctga tggccagcca
    12121 gtcctcagac tgacgtccga caggccgatc aacgatccgt ttgtcgacat gctgcttgaa
    12181 ctcaactggc ctgccggccg gctggttcgt gaatacacct tcctgcttga cccgcctgag
    12241 gtagctgcaa agggtttccc gccgcttgct ccggcgatgg ccaaaccggc aaccgcaagt
    12301 aaacaagctg tagccacaac acgcacgaaa tcggcaattg acgatgagtt gcgtagtaag
    12361 gcggtcgcca gaatccgtaa tccggggtca gcccagaaat ctgcagctca gccttcttct
    12421 tcagggccag acactctaga agtcaaacgc ggtgatacct tgcacaagat cgccggggaa
    12481 agcaaaccgg atggcgtttc gctcgatcag atgctggtgg gcctctttcg tgccaatcag
    12541 gatgctttcg acggcggcaa catgaaccgg ctgagggccg gaaaaattct tgtcgtaccc
    12601 gaaaaatcca ctgtcgaggc cgtttcttcg ggagaagcca gcaagatagt ccgcgctcag
    12661 gcctcggact ggaatgctta ccgaaacaaa ctggccggtg ttgcagcgca ggcgcccgtc
    12721 agtgatgagg ctgccaggca ggaggcaacc ggcaagataa gcgccaaagt tgaggacaag
    12781 gctgcgccat cagtcgaacc caaggatcag ctaaaggttt ccagaaccga gacaactgct
    12841 gccaaaccgg acgttggcgc caaacgcagc gaagaagacc ttatcgccaa ggaaaaagcg
    12901 ctcaaggaag cgaacgagcg tttgatcgct ctagagaaaa atgtttctga tttgcaaaaa
    12961 ctggttgaac tgaagaatca gaatctggcc gagttgcagg cgcaggcgtc atcaaaggcc
    13021 atgccgctcg aatcgataaa gcccacggaa gaaaccaagc cgccggcgac gacacctgac
    13081 ccggttcccg ttgcaccgcc ggcaaagata gagcccgttg cgaccgcgat tgaaaaacca
    13141 gcggaaaaac cggctgaaac gacgccacct gaaccggcgc ccaagcccga ggaacccaaa
    13201 cctgagccga agcctgcggt gaagccaaaa ccggcgattg ctccacctcc tccgcttgag
    13261 gagcccggtt tcatcgatgg actgctcgac aatccgctgg cactggccgg tggcggaggg
    13321 gttcttgcac ttctggctgc ctacttcgtt tctcgacgtc gtcgtgccgg caaggaggaa
    13381 gtgcctctgg attcgtccag caccctgtcg ccacagagta gcagcctcag cctgacggct
    13441 aattcggtct tccgcaacac gggaggtcag agtgtcgaca cctcgcacac tcctgctcaa
    13501 accgatttca gtcaagctgg tcccggtagc attgacaccg acgaggttga tccggtagca
    13561 gaagccgacg tgtacatggc ttatggccgc gatgtccagg ccgaagaaat acttgtcgag
    13621 gccaagcaaa aggatccgaa acgtcatgcc attcacttga agttgcttga gatctattca
    13681 aaccggaagg atctgaagca attcgaaacg cttgcgacgg atttgtatgg agatacaggt
    13741 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaactgga agaaacacac
    13861 agactgccgg aaccggtaat cgattccagc gccctcgatt ttgatcttgc ggcagacacg
    13921 ggaacatccg attcggccac tgacgcggat gcgacagtcg ttggccttga cttcgatctg
    13981 ccggatgctg ccaaggcaga agcttccgac gatggaacgg tggctacagc tcccgacctg
    14041 gactttgacc ttggtttcga accaagccct gctgaaacag ccgagcccaa gaccgattcc
    14101 ccggatagtt cgaacgctgc cgcctccgct gtcagcagcg atgacgttga tttcgatatc
    14161 agtatgaccg agtcgacctt cctgggccga ccgatgcctg aaccgtcgtc ctttgacatg
    14221 tcatcgatcg acctggatct taaagagccg gaagctgagg cttcggcatc gacaccgccc
    14281 ccttcagtag ctgagcccga ttcttcgtcc gatctgccag actcttcgtt cgatgacgtt
    14341 caagtgtcga cttctgttaa tccggacttc gagacagaac aatcggtgac gatcgtgaat
    14401 ccgcagtttg gtactgatca agatgacctg atgccagaac aggacttctc ttcagaacag
    14461 atggcaactg ccgtgaatcc gcagttcgac ggtgagcaga ctctcgtggc tggccgggac
    14521 ttctcggcga cgcaggaaga cactgtagtg aatccgcagt ttgatggcga acagactctt
    14581 gtgtccggcc gggacttctc ggcaactcag tcagaaaccg tcgtcaatcc acagtttggc
    14641 gatgaccagg atcttgcgcc agagttcgag atcagtccga acgaagaagt aacgacaaaa
    14701 cttgatttgg cgaaggccta cgaggaaatg ggtgatctgg aaggtgcacg ggaactcctg
    14761 caggaggttc tgaaagaagg cgatgccgca cagcaggaaa aagcgcaggc gattctcgcc
    14821 aagatcagcg agtaggacca gaagacggta tattggccgt agctctggtc tgagctacgg
    14881 cctttttcat tggtgctggt taggtcgcgc tgggcaggaa gaagcggaac gttgctgtcc
    14941 aacgtacctg ccactccttg cagccgattt ccgcagaccc gacaggccac ggaattcaag
    15001 catatttgta tcaagcatgc cgaaactgac catccacccg acgacttgtc tcattgtctg
    15061 gcttctgttt ctggttgttg tccagttcat gagcggaaca accctggtcg tgacccttct
    15121 gttgttaccc gctctcggaa cgtatgtcct gcagcgtggc agtcgcctga tttggcgagc
    15181 ccgctggctg ctgatttcga tgcttgccat cttttcgtgg ggaattcccg gcgatccact
    15241 ttggaacggt gcctttgcgc caacgcacga aggcttgcgt gaaggactga ctcactttgg
    15301 ccgcttgtta ctggtgctgg ttgccgtcgc tgctttcctg gaagcgatgc ccctccccga
    15361 gttgctggcc gccatgcata cattgctcgg gcctttgcgc cacttggggc ttgaccctga
    15421 tcgaggcgtc gtacgtctca tgctggttct gcgctatgtt gaggctctgc cccgtccacg
    15481 cgactggcga acattgatcg atgccccggc gacgaatctg ggagaagccg ttgaagtgaa
    15541 tcatcggccg cttcgctggc ctgattattt tgtcgcactt gcgctggctt tagctgcggc
    15601 gttttatgta tttcgatagg cgtacgggat gagaattgcc ctcggcgtcg aatatgacgg
    15661 gagcgctttc catggctggc agaaacagcc ggacggaaat acagtacagg acgccctgca
    15721 gtcggcgctg agcgggattg ccggcgtacc aattcaagtg gtagccgccg gccggacaga
    15781 caccggggta catgcgacgg gccagattgt gcacttcgat acatggattg aacgcccttt
    15841 gaacgcctgg gtgcgtggca ccaacactta tctacccgcg gcagtagcgg tgcgttgggc
    15901 acatcctgtc agcgacagct ttcatgcccg cttttccgct tacggtcgcc gctatcgcta
    15961 tatcgtgatc aatcggccac aacggacagg tgtgtggcaa agacatgcgg gctggtatca
    16021 tcacccgctt gacgtcggaa gaatgcgaca ggcggcccga atgctagtgg gtgagttcga
    16081 cttttcggcg ttccgcgcgg cggattgcca agccaaatca ccggtcaaga caatgcggca
    16141 agcggatgta cagcggtacg gcgacacgat tgtcttcgac ttcgaggcag gagctttcct
    16201 gcaccacatg gtacgcaatc tggtgggcag tctggtctat atcggccagg gcaagcaccc
    16261 accggaatgg atgcagcagt tgctggcctc gggtgaccgt cgtattgcgg cgccgacctt
    16321 ttctgctgcg ggattgtatc ttgtcggtgt caaatacgag ccgcactggg gcttgccgaa
    16381 cagcgacgat ttctttttgc caggcatggc cggcttgtga gatgcgtgct aacaggagta
    16441 ttggggctat ttcgatggaa aaaccacgca tcaagatatg tggtctgacc cgacccgagg
    16501 atgtgcaggc tgcagtggac gctggagccg acgcgattgg tctggtcttc tatccaccga
    16561 gtccgcgtta cgtcgcgctg gaaacggcag ccgagctggc acgaactgtc cctccgttcg
    16621 taaccatcgt cgggctattc gtcaacgccg ccccggaggt ggtaaaagaa acactggcgg
    16681 ctgtgcccat ccacatgttg cagtttcatg gtgacgagga tgaagtctac tgccgacagt
    16741 ttgcccgacc ctatatgaaa gcggcgcgcg tgaagcctgg actggatttg gtacaatacg
    16801 tagttggctt cccttcggca caggcgattc tgcttgatgc ctttgttgaa gcttacggtg
    16861 gcggtgggca ggtttttgac tggacgctga tcccccccgc gttaggcaag ccgattgttc
    16921 tctccggcgg cctggatgcg cgcaatgtcg gtgatgccat cattcgtgtg cggccggcag
    16981 cggttgatgt ctcatcgggc gttgaggcga ccaaagggat caaggatgcc gacaagatcc
    17041 gtgcttttgt taccgctgtt cgtgcagcag gtttgatgcc gaccgattga ttcaggaata
    17101 ccgaacaaaa accacgttcg gactaggaaa accgatgcgc aaaagttcag gtggacgtac
    17161 tcgtacttgc tggcgacgcc atttcgccaa ccatgcttga ggcagccgat cgttttttcg
    17221 tctgcgtgca ttgaattgca attgactcaa ggagatacca tggttgcccc ccgttatgat
    17281 ttgcccgatt cgctgggcca cttcggcccg tacggcggcg tattcgtcgc cgaaacactg
    17341 attcccgcac tcgaagaact gaaagaagcc tatgcgaatg cacagcgcga cccgctgttc
    17401 attgccgaat tcgattacga cctcaagcat tacgtgggtc gaccgagtcc tatctaccat
    17461 gccaaacgct ggtccgaatt gctcggcggt gcgcaaattc atctgaagcg cgaagacctg
    17521 aatcacaccg gcgcgcacaa gatcaacaac tgcattggcc aggccattct cgcccggcgc
    17581 atgggcaagc cgcgggtcat tgccgaaacg ggcgccggcc agcacggcgt cgccaccgcc
    17641 accgtggccg cgcgctacgg catggaatgc gtggtctata tggggtcgga ggacatcaag
    17701 cggcaggccg ccaacgtcta tcgcatgaaa ttgctcggag cgaccgtggt acccgtcgat
    17761 agtggttcca ggacgctcaa ggacgcgctg aatgaagcga tgcgcgactg ggtcaccaac
    17821 atttccaaca ccttctacat catcggcaca gtcgccggcc cgcaccccta cccgatgatg
    17881 gtgcgcgatt tccagtcaat catcggccgg gaagccatcg ggcagatgca ggaacaacat
    17941 ggccgtcagc ccgacgccgt catcgcctgc gtcggcggcg gctcgaacgc catggggata
    18001 ttttatccct acattcccgt cgagggtgtg cgtctgatcg gcgtcgaagc cgccggcgac
    18061 ggcatcgaga ccggacacca cgcggcttca ctgacggccg ggcgccccgg cgttttgcac
    18121 ggcaaccgga cgtatctgct gcaggacgag aatggccaga tcatcgaaac gcattcgatt
    18181 tcggccggcc tcgactaccc cggtgtcggc cccgaacacg cctggctcaa ggattccgga
    18241 cgcgccgaat acgtcaccat caccgatgcc gaagccctgc aagccttcca cgatctatgc
    18301 cgccaggaag gtatcatccc ggcgctcgaa tcgagccatg ccctcgccta cgccgccagg
    18361 ctcgcgccaa gtttgccgaa agaccggat
//
LOCUS       DAORPM010000013        18164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_554_length_18164_cov_11.095974, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000013 DAORPM010000000
VERSION     DAORPM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 18164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18164
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_18164_cov_11.095974"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PLD53_01310"
     CDS             <1..726
                     /locus_tag="PLD53_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC59572.1"
                     /translation="CAIWRFEQGNRSRLTRYATLGLADGAAGLARLQWSERRFVESGG
                     HLLFAQHDYPLRLSDIAFVKVGAVSGADDLYTSNELGNRDFVCSSTVADGLTRRMIWC
                     EPGDRPVDALLPHKERLIARRIRHFDEFNWWQWGRSYYQSEQARVYVNGKTRRSRPFF
                     AHDCLNYDGSVLAVFPHDKTLDVRLLAEALNSVDWSDLGFVCDGRFLFTQRSLEQSPL
                     PASFRSFLPPGGVTWWDKLKKLL"
     gene            768..2084
                     /gene="gshA"
                     /locus_tag="PLD53_01315"
     CDS             768..2084
                     /gene="gshA"
                     /locus_tag="PLD53_01315"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRC59573.1"
                     /translation="MVPHLTTALTGPLLALEANFLDATPDIERWLRGMWQEHTPPFYG
                     SVDLRNSGFKLAPVDMNLFPGGFNNLNSAFLPLCVQAAMTAIDKICPSAKSLLLIPEN
                     HTRNLFYLQNVAQLVAILRLTGLNVRIGSLLPEIVKPTPIELPNGTTLLLEPLVRSQY
                     RLGLEGFDPCVILLNNDLSAGIPAVLQSLEEQFVLPPLHAGWAVRRKSTHFAAYDEVA
                     GEFAQLVGIDPWQINPYFAVCSSVNFHERQGEECLSSSVDAVLGLIRKKYKEYGIEET
                     PYVVVKADAGTYGMGIMTVKDASEITGLNRKQRNKMSVIKEGVNVSQVIIQEGVHTHE
                     RVGKKDQEGVAEPVVYMIDRYVVGGFYRVHSGREKDENLNAPGMRFEPQAFETSCSLP
                     DQCQNPDAPPNRFYAYGVVARLAQLAASLELERTTPAAVEDMAVSL"
     gene            2128..3096
                     /gene="gshB"
                     /locus_tag="PLD53_01320"
     CDS             2128..3096
                     /gene="gshB"
                     /locus_tag="PLD53_01320"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HRC59574.1"
                     /translation="MRFAFIIDPLDSLKAYKDTSVAMMRAAQAAGHAVWVIQQSAIHW
                     SSQHGVCADASHLVMLEDDTEWFVVMDRHVVPLRDFAAVVMRKDPPFDIEYVTTTWLL
                     SRAEIEGARIFNRPQALRDHSEKLSVAEFAQFAVPTLVSREPALIHSFIDTQHDVILK
                     PLDGMGGSQIFRVRNDDPNRNVIVETLVEDPLSHDGTRTIMAQRYIPEITDGDKRILL
                     VGGELVPYCLARIPKAGETRGNLAAGGSGVARELTARDREIAGALAPVLAERGLLLVG
                     IDVIGDWLTEINVTSPTCMVEIFQQTGFDAAQMFIAALERECIVPV"
     gene            3078..4154
                     /locus_tag="PLD53_01325"
     CDS             3078..4154
                     /locus_tag="PLD53_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRC59575.1"
                     /translation="MHCARLAVLLALLLSLAACTKAQLHHQESFVFGTRVEVLIAGLD
                     DAQARPAAAAVLREFDRLHRTYHAWQPSELSALNDAIAAGKALEVTPEMAGFLVDAQR
                     IAAAGDHLFDPGIGNLIELWGFQSDEFKATLPDPVKLAAWRAAHPGMADLRIEGTSVS
                     SRNRAVAIDFGGYLKGVALDRATAILHERGVNNALINIGGNVMALGTKNGERWRVGIQ
                     HPRQPGPLATVELNDGEAIGTSGDYQRYFELDGKRYCHLLDPRSGGPAMQTQALTVLI
                     TPRPAAGTLSDAASKPLFIAGADWPELARKLAIDQVLRVDAEGRIQVSSALHRRLEFV
                     GTEPKRLDIFSLDGSRPHGIRGIP"
     gene            4185..4574
                     /locus_tag="PLD53_01330"
     CDS             4185..4574
                     /locus_tag="PLD53_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HRC59576.1"
                     /translation="MIGILLITHGTFGESLIQNVCHVLNKRPPLIGQLGVAAQDDLLD
                     VLPMAELLLKEVDQGEGVLILTDVFGATPANLGLKLLSPGRVEGIAGVNLPMLLRALT
                     YRDRGMSILLQKAVSGGHDGVINMRQH"
     gene            4597..4866
                     /locus_tag="PLD53_01335"
     CDS             4597..4866
                     /locus_tag="PLD53_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRC59577.1"
                     /translation="MARAEIEISNKLGLHARASAKLTQLAGSFHSEVWLEKGSRRINA
                     KSIMGVMMLAAGKGSTVVVETIGNDEQPALESLLKLIGDKFGEGE"
     gene            4874..6616
                     /gene="ptsP"
                     /locus_tag="PLD53_01340"
     CDS             4874..6616
                     /gene="ptsP"
                     /locus_tag="PLD53_01340"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRC59578.1"
                     /translation="MSFTLHGLAASSGIAIGQAHLISQATLEVSHLVISPRMVEKEVA
                     RFETVLEQVRSEFATMKSEMGKSPTDIGAFIDLHMMILADPELSEVPKQIIRERRCNA
                     EWAIVQQMEFLVAQFEKIGDAYLRERSYDVRQVVERVVRVLIGRPSHSAVKVPKGVRG
                     ENLIVVAHDLSPSDVMAFKDQQFASFVTDVGGITSHTAILARGMGVPAVLGLHNARQL
                     IRDKETIIVDGTRGVIIVDPDPRILEEYELKKSQIEIDRSKLKLLKSTRATTLDRVKI
                     DLLANIEEPSDVAAALDSGAEGVGLFRTEFIFLGRGDMPSEDEQFEAYRTAVKAMQGR
                     PITIRTFDLGNDKNLHSDDIIGGRRRDNPALGLRSIRLSLAEPASFQAQLRAILRVSK
                     LGKVKILIPMMSHAHQIDQTLAALEQAKSSLRGGRIAFDENIEIGGMIEVPAAALAVG
                     IFLRRMHFLSIGTNDLIQYTLAIDRSDEQVSNLYEPLHPAVLMLLAHVISTAEKVNIP
                     VSMCGEMAGDPNLTRLLLGMGLREFSMHPSQIPAVKQKVKQSDISELIPITRRILRLE
                     ESPKIREQLDRLNS"
     gene            6842..7969
                     /locus_tag="PLD53_01345"
     CDS             6842..7969
                     /locus_tag="PLD53_01345"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRC59579.1"
                     /translation="MSQQNSVGIVAPQRAHFNEPIALKSGALLPGFELVFETYGTMNT
                     ARSNAVLVCHALSGGHHVAGVDASDPTNVGWWDNLVGPGKPLDTDKFFVVGVNNLGGC
                     YGSTGPLSVNPITGKRYGADFPMVTVEDWVAAHARLADHLGIDTWAAVIGGSLGGMQA
                     LEWALQYPDRIRHAMVIASAPKLSAQNIAFNEVARQAILSDPDFHGGHYAEHGVVPVH
                     GLRLARMVAHITYLSDSQMGEKFGRQLRHGEHKFSFDVDFEVESYLRYQGDKFASFFD
                     ANTYLNTTKALDYFDPAYAYDGDLAAALARAKAKFFVASFSTDWRFSPARSREIVFAL
                     LHNRRHVAYAEIDSDAGHDSFLLDDAYYHAVLRAYLENISV"
     gene            7966..8586
                     /gene="metW"
                     /locus_tag="PLD53_01350"
     CDS             7966..8586
                     /gene="metW"
                     /locus_tag="PLD53_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRC59580.1"
                     /translation="MSNGNPLTAIERERFDFAVIANWIPPGERVLDLGCGDGKLLRYL
                     RESKGVVGYGVEISHESVLGCIRNGVDVIQIDIEGGLSGFENRSFNHAIISQALQAMH
                     ATERILGEMLRVAEEAVVSFPNFAYRANREAIAQGHMPVSENLPFEWYETPNVRFFTI
                     ADFDALCDKLGIEIRERLAFDDAGQPVTDDPNLNGSLAFYRLGRKA"
     gene            8586..9860
                     /locus_tag="PLD53_01355"
     CDS             8586..9860
                     /locus_tag="PLD53_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HRC59581.1"
                     /translation="MPAWLSALLTRKMLICVFTGFSSGLPLYLLLNLLPAWLRSEGVN
                     LKTIGIFALIQFPYTWKFLWSPFLDRYALPVLGRRRSWSLIMQIGLLLTIGLLGGLSP
                     STDIVPVLWLTTFLALFSATQDIALDAYRRELLTENELGLGNAVHVNAYRVAGLVPGS
                     LSLILADLLPWSQVFWITAAFMLPGMVMILLVQEPTNAYARPKTLREAVVEPFHEFVG
                     RSGLHGALLVLAFIFLYKLGDSLCTALATPFYLDMGFTKTDIGLIAKHAGLWPAVFGG
                     LLGGLWMVRLGINRALWLFGVVQLLSILGFAWLAWLGPQTDIGVDQRLALATVIGFEA
                     LGVGLGTVAFVAFIARSTHPAYTATQYALFTSLAAVPRTFFNASAGWLVESLGWYSFF
                     LLCTALAIPGMLLLPRVAPWKIVPVKGAQVGV"
     gene            9850..10587
                     /locus_tag="PLD53_01360"
     CDS             9850..10587
                     /locus_tag="PLD53_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRC59582.1"
                     /translation="MASEASASSAELLIAAARELSARVEKLTFALPVSHIYNPLAYAW
                     EAHELYLRRYGACRKKVVFIGMNPGPFGMVQCGVPFGEIAAVRDWMGIRTQIDKPAME
                     NPKRRIEGFSCTRSEVSGRRLWGLFQQRFGKAEAFFANHFVANYCPLAFFDSGRNITP
                     DKLPSAEKEPLNAACDRHLRQLVTALEAQWVIGVGGFAEKRATEALTGMDVSIGKVLH
                     PSPASPAANRGWAEAATRQLIAQGVWH"
     gene            complement(10647..10955)
                     /locus_tag="PLD53_01365"
     CDS             complement(10647..10955)
                     /locus_tag="PLD53_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59583.1"
                     /translation="MAKASVVSRMVASQKRQARGFVADVVRMRGLMPLLMKNRNGDKW
                     TANEKSELLDQLRVLSRVSPYLLFLLLPGSALLLPVYAWWLDRRRVRRAARPVSETLP
                     "
     gene            complement(10982..11302)
                     /locus_tag="PLD53_01370"
     CDS             complement(10982..11302)
                     /locus_tag="PLD53_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HRC59584.1"
                     /translation="MNQRLNELLIRRGRLLERIATQRAALGQEMPPLRAALYTTDRIV
                     AGVRSGVVFIKQHPSIVTVAVAVLVVLKPGRIVRWAKRAFFTWQTWRTLRDKLPIFGL
                     KAGL"
     gene            complement(11283..11693)
                     /locus_tag="PLD53_01375"
     CDS             complement(11283..11693)
                     /locus_tag="PLD53_01375"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRC59585.1"
                     /translation="MSDRSGRGNSGLFHALKSIVVTLLASGKTRLELLGNELEAEKLR
                     AIHLILMAQGMVFCFGVCTLLIVALLTALFWDNRLLVLSVSTGLFLVLSGVFFALFKR
                     SLQRPEQVFATSIAELKEDLRQLKEAVGHESAAE"
     gene            complement(11707..12027)
                     /locus_tag="PLD53_01380"
     CDS             complement(11707..12027)
                     /locus_tag="PLD53_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HRC59586.1"
                     /translation="MTEVNEMSNNSKQKFVSDMKSVVADAEEILRATAGVAGEKMADL
                     RERVGERLRDAKLRIADAEALLVDKTKAAARATDDYVNQNPWQAVGVAAGIGFLFGII
                     IGRR"
     gene            complement(12150..12932)
                     /locus_tag="PLD53_01385"
     CDS             complement(12150..12932)
                     /locus_tag="PLD53_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRC59587.1"
                     /translation="MLRIINLNLNGIRSAWRKGVLPWLSAQNADVVCLQELKAQFADL
                     SDEMKAPAGLHAYYHCAEKKGYSGVGLWCRRKPDAVTEGFGHAEFDAEGRYLRADFGP
                     LSVISLYLPSGSSSPERQAAKFSFMDVFHPHLLDLAKSGRDVVVCGDWNIAHQEIDLK
                     NWKSNQKNSGFLPEERAWVSQVLNEGNWCDVYRQLHATSTDECYTWWSNRGQAWANNV
                     GWRLDYQLASPALARTAHAAAVYKAQRFSDHAPLQIDYDYVL"
     gene            12968..13615
                     /gene="pyrE"
                     /locus_tag="PLD53_01390"
     CDS             12968..13615
                     /gene="pyrE"
                     /locus_tag="PLD53_01390"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRC59588.1"
                     /translation="MDFRQEFIEFALEQDVLRFGEFKTKAGRLSPYFFNAGLFNDGAA
                     LDRLSQFYAKAILASGLAVDTLFGPAYKGIPLVAVSALALARAGRNLPFSFNRKEAKD
                     HGEGGSIIGAPLAGRVLIIDDVISAGTSVRESVELIRAAGATPCGVVIALDRMERGRG
                     HLSATQEVERDFAIPVVAVATLDDLMAFLRERSDFKEHQSAVTLYRQEYGIASAH"
     gene            13599..14225
                     /locus_tag="PLD53_01395"
     CDS             13599..14225
                     /locus_tag="PLD53_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59589.1"
                     /translation="MHPLIKLGVLAIALALSGSGMAAGKLYCCDDAKGMQVCGDLLPH
                     ACYGRAYREIGASGRTLRNVAAPLTAEERAQQALEEAKRKEEEAALKEQQRKDQALLN
                     TYGSVKDIEAMRSRAQDDVQRSIAAAQEKIAEIRAQRKQYEDEAEFYKKKQLPAAVQK
                     GLNDTAFEITAQESIIESKQKELKIIREKYDEDKRRYIDLSRRGLTRQ"
     gene            complement(14265..15719)
                     /gene="gatB"
                     /locus_tag="PLD53_01400"
     CDS             complement(14265..15719)
                     /gene="gatB"
                     /locus_tag="PLD53_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRC59590.1"
                     /translation="MSKWEVVIGIETHAQLATNSKIFSGSSTDFGAAPNVQANAVDIA
                     LPGVLPVLNKGAVECAIRFGLAVDGEVAAKSVFARKNYFYPDLPKGYQISQYELPVIV
                     GGRLAIQLGQGDSLSEKVITLTRAHLEEDAGKSLHEDFHGKSGIDLNRAGTPLLEIVS
                     EPDMRSSAEAVAYAKALHALVRWIGICDGNMQEGSFRCDANVSVRPVGQQKLGTRREI
                     KNLNSFRFMQQAIDYEVQWQIAEIEEGRSIRQATVLFDAGTGATSAMRSKEDAHDYRY
                     FPDPDLLPLLIDRDWVERTRASMPELPVAKRDRFVAQYSLSSYDATTLTASREVAGYF
                     EEAVAVGGIGSAKICANWVTVDLAARLNKEGREIGESPVSPHQLGGLVARIADKTVSN
                     NIAKKVFESLWSDEGKTADDIIEQHGLKQITDSSAIEVLINGVLAANASMVAEFKAGK
                     EKAFNALVGQAMKATKGKANPQQVNELLRKKLGE"
     gene            complement(15737..16255)
                     /locus_tag="PLD53_01405"
     CDS             complement(15737..16255)
                     /locus_tag="PLD53_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59591.1"
                     /translation="MRLIFATLLALLLGACATPETASPDSSGQVAAGASQPTEKIRSQ
                     PLKHLLGRTLKPMPDKALDVRTKCRFRDVAGGRGAMDLQVTKAEVKRFVAEVSIPKQG
                     VCHFDMKYFKQTESFPNVVLTDGASGCVVRMWEQEKGVTVAFNACQSKCGGDAFSYLW
                     PILVDTRNGRCT"
     gene            complement(16261..17754)
                     /gene="gatA"
                     /locus_tag="PLD53_01410"
     CDS             complement(16261..17754)
                     /gene="gatA"
                     /locus_tag="PLD53_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRC59592.1"
                     /translation="MIARNVSALSQALAAKEISSVELTQLYLDRIARLNPEINAFVTV
                     DADKSLAQARAADESRAVGKAGKGAGPLTGIPLAQKDIFCTKGWLTTCGSRMLGNFVS
                     PYDATVIENYNAAGAVILGKTNMDEFAMGSSNETSYFGAVKNPWDTARVPGGSSGGSA
                     AAVAALMAPAATGTDTGGSIRQPAALCNLTGLKPTYGVVSRYGMVAFASSLDQAGPMG
                     RSAEDCALLLNAMAGFDERDSTSLSREKEDYGREISAAAGDKPLAGLKIGLPKEFFGT
                     SERSGCSPEVMQLVDAAIAEYRKLGAETVDISLPNMKLSVPAYYVIAPAEASSNLSRF
                     DGVRYGYRAPEYSDLNEMYARSRAQGFGVEVKRRILIGTYVLSHGYYDAYYLQAQRVR
                     RLIANDFVAAFRQCDVIMGPTSPSTAFNLGEKAADPVQMYLSDIYTIAVNLAGLPGMS
                     IPCGFASSLPAGLQLIGDYFSEAKLLNVAHRYQQTTDWHLRHPSGFD"
     gene            complement(17760..18047)
                     /gene="gatC"
                     /locus_tag="PLD53_01415"
     CDS             complement(17760..18047)
                     /gene="gatC"
                     /locus_tag="PLD53_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRC59593.1"
                     /translation="MSLTLEQVRRVAHLARIEVSDAEAETTLGHLNGIFGLIEEMQAV
                     DTRGVEPMAHAQDLAQRLRSDRVTEEDCRDAFQAIAPEVEAGLYLVPKVLE"
BASE COUNT         3792 a         5354 c         5151 g         3867 t
ORIGIN      
        1 tgtgcgatct ggcgctttga acaaggcaat cgctcacgac tcacccgata tgccaccctg
       61 ggcttggcag atggcgcggc gggactggca cgcttgcaat ggagcgagcg gcgtttcgtc
      121 gaaagtggcg gtcacctgtt gtttgcgcaa cacgactatc cgttgcgtct gtccgatatt
      181 gcgtttgtga aagtcggtgc agtatccggc gccgacgatc tctacaccag caatgaactc
      241 ggcaatcgcg acttcgtctg ttcatcaacg gtggccgacg gtttgacacg ccggatgatc
      301 tggtgcgagc caggcgaccg gccggtggac gcactgttgc cgcacaagga acgcctgatt
      361 gcacgtcgaa tccgccactt cgatgaattc aactggtggc agtgggggcg cagctactac
      421 cagtcggaac aagcccgcgt ttacgtcaat ggcaagacgc gccggtcacg acccttcttt
      481 gcccatgact gcttgaacta cgacggctcg gttctggccg tttttccgca cgacaagacg
      541 cttgacgttc ggttgctggc ggaggcgctg aacagcgttg actggagcga tcttggcttt
      601 gtttgcgacg ggcgcttcct ctttacccag cgtagccttg agcagtctcc gctacccgcc
      661 tccttccgct cgttcctgcc gccgggcggt gtaacatggt gggacaaact caaaaagctg
      721 ctatgagaaa tattgatcgt gctccaaggc atgatcggag ataagccatg gttcctcatc
      781 tgacaaccgc tctcaccggc cctcttctgg cactggaggc gaacttcctc gacgccactc
      841 ccgatatcga acgctggctg cgcggcatgt ggcaggagca tacgccgccg ttctatggat
      901 cggtcgatct gcgcaactcc ggtttcaaac ttgcgccagt cgatatgaat ctgtttcccg
      961 gcggtttcaa taacctgaat tcggcctttc tgccgctctg cgtgcaggcc gcgatgacgg
     1021 ccatcgacaa aatctgtccg agtgcgaaga gcctgctgtt gattcccgaa aaccacacgc
     1081 gcaacctttt ctatcttcag aatgtggctc agttggtggc cattctgcgc ctgacgggtc
     1141 tgaacgttcg tatcggatcc ctgctgccgg aaatcgtcaa gccgacgccg atcgagctgc
     1201 ccaacggcac cacgctgctg ctcgaaccgc tggtgcgcag ccaatatcgc ctgggccttg
     1261 agggcttcga tccctgcgtc atcctgctca acaacgattt gtcggccgga attcctgcag
     1321 tacttcagag tcttgaagaa cagttcgtgt tgccgccctt gcatgccggc tgggccgtac
     1381 gccgcaaatc gacgcatttt gctgcctacg acgaagtggc tggagagttt gcccagttgg
     1441 tgggaatcga cccctggcag atcaatccat acttcgcagt ctgcagcagc gtcaattttc
     1501 atgaacgtca aggcgaggaa tgcctgtcgt ccagcgttga cgcggtactc ggcctgatcc
     1561 gcaagaaata caaggaatat ggcatcgaag aaacaccgta tgtggtggtc aaggccgatg
     1621 ccggcaccta tggcatgggt atcatgacgg tcaaggatgc ctccgaaatt accggactga
     1681 accgcaagca gcgcaacaag atgtcggtca tcaaggaggg ggtgaacgtc tcgcaagtga
     1741 tcatccagga gggcgtgcat acccacgaac gggttggcaa gaaagaccag gaaggcgtcg
     1801 ccgaaccggt tgtatatatg atcgaccgct atgttgttgg tggcttctac cgcgttcact
     1861 cgggccgcga gaaggatgaa aacctcaatg cgcctggcat gcgtttcgaa ccgcaggcct
     1921 ttgaaaccag ttgttcgctg cccgaccagt gccagaaccc ggatgcaccg cccaaccgct
     1981 tttacgccta cggcgtggtg gcgcgactgg cgcagcttgc cgcgtcgctt gagctggaac
     2041 gtacgacacc ggcggcagtg gaagacatgg cggtgtcgtt gtagctgtgc ttccaatcta
     2101 cccagccccc gcccaggagg aatattcatg cgtttcgctt tcattatcga cccgcttgat
     2161 tcgctgaagg cctacaagga tacaagcgtc gccatgatgc gcgcggcgca ggcagccggc
     2221 catgccgtgt gggtgatcca gcagtccgcc attcattggt cgtcgcagca tggcgtgtgc
     2281 gccgacgcgt cacacctcgt catgctggaa gacgatacgg agtggttcgt ggtaatggac
     2341 cgccacgtcg tgccactgcg tgatttcgcc gccgtggtca tgcgcaagga tccccccttt
     2401 gacatcgaat atgtcacgac cacctggctg ctttcgcgcg ccgaaatcga aggggcacgc
     2461 atcttcaacc gaccgcaggc gctgcgcgac cattccgaga agctttccgt ggcggagttc
     2521 gcacaatttg ccgtgcccac tctggtatcg cgggaaccgg ctctgattca cagcttcatt
     2581 gacacacagc acgatgtcat tctgaagccg ctagatggca tgggcgggag ccagatcttt
     2641 cgcgtccgca atgatgatcc gaaccgcaac gtcatcgtcg agacattggt cgaggatccg
     2701 ctatcgcatg atggcacgcg aacgatcatg gcgcagcgct atatccccga gatcaccgat
     2761 ggcgacaagc gcattctact cgtcggcggc gaactggtgc cctattgtct ggcgcgcatt
     2821 ccgaaggccg gcgagacgcg cggcaatctg gccgccggtg gcagtggcgt ggcgcgcgag
     2881 ctgacagccc gcgaccgtga aattgccggc gcactggcac cggtacttgc cgagcgcggc
     2941 ctgctgctcg tcggaatcga cgtgatcggg gactggctga cggaaatcaa tgttaccagc
     3001 ccgacctgca tggttgagat tttccagcaa accggttttg atgccgcaca gatgttcatc
     3061 gccgcgctgg agcgtgaatg cattgtgccc gtttagccgt tctgctggcc ctgctcctgt
     3121 ctctggcagc ctgtaccaag gcgcaactgc accatcaaga gtccttcgta ttcggaacac
     3181 gtgtcgaagt tctaatcgcc gggctagacg atgcgcaagc gcgcccggca gcggccgccg
     3241 tgctgcgcga attcgaccgc ttgcaccgta cgtaccacgc ctggcagccg tccgaactga
     3301 gcgctttgaa cgacgccatt gctgccggca aagcgctgga agtgactccc gaaatggccg
     3361 ggtttctggt cgatgcacag cgcattgccg cagccggcga tcacctattc gaccctggca
     3421 tcggtaacct gatcgagctg tggggtttcc agtccgatga gttcaaagcc acccttcccg
     3481 atccggtaaa actcgctgca tggcgcgcgg cgcatcctgg catggccgat ctgcgtattg
     3541 aaggtacgtc tgtaagcagc cgcaatcgtg ccgtcgccat cgacttcggt ggctatctca
     3601 aaggcgttgc gttggaccgt gcaacggcca tcctgcacga gcgaggtgta aacaacgcac
     3661 tgatcaacat cggcggcaac gtaatggcac tcggcacgaa aaacggcgag cgctggcgcg
     3721 tcggcatcca gcatcctcgt cagccgggac cgttggccac ggtcgaactc aacgacggcg
     3781 aggcgattgg cacttccggt gactatcagc gctactttga gctggatggc aaacgctact
     3841 gccacctgct tgacccgcgt agcggcgggc cggccatgca gacccaggcg ctgaccgtgc
     3901 tcatcacgcc acgccctgcc gccggcacgc tctccgatgc ggcgtccaag ccgcttttca
     3961 tcgctggagc cgactggccc gaattggcaa ggaagctggc aatcgatcag gtattgcggg
     4021 ttgatgccga gggccgcatc caggtcagct cagcactgca tcgtcgcctt gaatttgtcg
     4081 ggactgaacc gaaacggctc gatatttttt cgcttgacgg ttccaggcct cacggaatac
     4141 gtggaatccc ttagaatgtc ggctgatttc caccggaacg cacgatgata ggcattcttc
     4201 tgattacgca cggcacgttt ggcgaaagcc tgattcagaa tgtctgccac gttctaaaca
     4261 aacggccgcc actgattggc caactcggcg ttgctgcgca agacgacttg ctcgatgttc
     4321 tgccgatggc cgaactgctg ttgaaggaag tcgatcaagg cgaaggcgtg ttgatcctga
     4381 ccgacgtctt tggcgcaacg ccggctaatc tcggcctgaa gttgctgtcg ccgggtcggg
     4441 tcgaaggcat tgccggcgtc aatctgccaa tgctgttgcg cgctctgact tatcgggatc
     4501 gcgggatgag catcctcctg caaaaggcgg tgagtggtgg tcacgatggc gtcatcaaca
     4561 tgcgccagca ctagcggatt atcgacagga aaattgatgg cacgagctga aatcgaaatt
     4621 tccaacaaac tgggcttgca tgcccgtgcc tcggccaagc tgacccagct ggccggcagt
     4681 tttcattccg aagtctggct ggagaagggg agtcgccgaa ttaacgccaa aagcattatg
     4741 ggtgtgatga tgctggcagc aggcaaaggt tcaaccgtcg ttgtcgagac catcggcaac
     4801 gatgaacaac ccgcactcga gtccctcctt aaactgatcg gagacaaatt tggcgaggga
     4861 gagtgatcaa accatgagtt tcactttgca cggtctcgcc gcatcgagcg gcatcgcaat
     4921 cggtcaggcg cacctgatct cgcaagccac gcttgaggtg tcgcacctgg tcatctcgcc
     4981 acgcatggtc gaaaaagaag tcgcgcgttt tgaaaccgta ctggagcagg tgcgaagcga
     5041 gttcgcgaca atgaagagcg agatggggaa atctccaacc gacattggcg ccttcatcga
     5101 cctgcatatg atgattcttg ccgacccgga attgtccgaa gtgccgaagc agatcatccg
     5161 tgaacgtcgt tgcaacgccg aatgggcaat cgtgcaacag atggagtttc tggtcgccca
     5221 attcgaaaag attggcgacg cctacctgcg cgaacgcagc tacgacgtgc gccaggtcgt
     5281 cgaacgggtc gtcagagtgc tgatcgggcg gccaagtcat tctgcggtca aggtacccaa
     5341 gggggtccgg ggagaaaacc tgattgtcgt cgcccacgac ctttctcctt ccgacgtcat
     5401 ggcgttcaag gatcagcaat tcgcttcctt tgttaccgac gttggcggca tcacttcgca
     5461 cactgccatc ctggcacgcg gtatgggcgt cccggcggtg cttggtctgc acaacgcccg
     5521 ccagctgatt cgcgacaagg aaacaatcat tgtcgacggt acacgcggcg taatcattgt
     5581 cgaccctgat ccacgcattc tggaagaata cgagctaaag aagagtcaga tcgagatcga
     5641 tcgctcaaaa ctcaaactgc tcaagagcac gcgggcaacg accctcgatc gcgtcaagat
     5701 cgatctgctc gccaacatcg aggaaccgag cgatgtcgca gcagcgctcg acagcggcgc
     5761 cgaaggggtc ggtctgttcc gtaccgagtt catttttctc ggacgtggcg acatgccatc
     5821 cgaggatgag cagttcgagg cctaccgtac ggcagtcaaa gcgatgcaag gaagaccgat
     5881 aacgatccgt acctttgacc ttggcaacga caagaacctg cattcggacg acataatcgg
     5941 gggccggagg cgcgacaacc cggcgcttgg cctgcgctca attcgcctct cgctggccga
     6001 acccgcatcg ttccaggcgc agttgcgcgc cattctgcgc gtctccaagc tgggcaaagt
     6061 aaaaattctg atcccgatga tgtcgcacgc gcatcagatc gaccagacgc tggctgcgct
     6121 ggaacaggca aaatccagcc tgcgcggagg aagaatagct ttcgacgaga acatcgagat
     6181 cggtggaatg atcgaggttc cggcagcggc actggcggtc ggaatcttcc tgcgccgcat
     6241 gcatttcctt tcgatcggaa ccaatgactt gatccagtac accctggcca ttgaccgcag
     6301 cgatgagcag gtttcgaacc tgtatgagcc gctgcacccc gccgttctca tgctattggc
     6361 gcacgtcatc agtaccgcag agaaagtcaa cataccggta tcgatgtgcg gcgaaatggc
     6421 tggcgacccc aacctgaccc gtttactgct gggcatgggc ctgcgcgaat tttcgatgca
     6481 tccgtcgcaa attccggcag tcaagcagaa agtcaagcag agcgacatat ccgaactgat
     6541 accgatcaca cgccgcattc tgcgcctgga ggaatccccg aaaattcggg aacaacttga
     6601 tcgactcaat agctgatggc ggacacccaa gctacgtaaa actgtttgct gtttgccgtt
     6661 cgctattgcc tggtcggtgg caccccgacg aatttgactt ttccaaggac tgccggtaag
     6721 tttccgaacc agcgccgatt tgaatcatca gcttgcgggc gcttaataaa tacggctaaa
     6781 gcgggaccag cccagtccgc tttgtcaagc cgactgggtt ttttcttccg ataagttcac
     6841 gatgtcacaa caaaattctg tcgggatcgt tgcgccgcaa cgggcccatt tcaatgagcc
     6901 gattgcactc aagagcggtg cactgcttcc aggcttcgag ttggtatttg aaacctacgg
     6961 tacgatgaat accgcccgtt ccaatgccgt gctggtatgt cacgcccttt ccggcggtca
     7021 tcatgttgcc ggcgttgacg ccagcgaccc cacgaacgtt ggctggtggg ataacctggt
     7081 tgggcccggc aagcctctcg ataccgacaa gttctttgtc gtcggggtca acaacctcgg
     7141 cggctgctac ggttcgacag ggcccttgtc ggtcaacccg attaccggca aacgctatgg
     7201 ggcggatttt ccgatggtga cggtcgagga ctgggtagcg gcgcacgcgc gtctggccga
     7261 ccatctgggc atcgacacct gggctgcggt catcggcggt agcctcggcg gcatgcaggc
     7321 actcgagtgg gccttgcagt accccgatcg catacggcat gccatggtga ttgcttccgc
     7381 gcccaagctg tcggcgcaaa acattgcttt caacgaggtc gctcgtcagg ctattctttc
     7441 cgatcccgac tttcatggcg gccattacgc cgaacacggc gtcgtgccgg tccatggcct
     7501 gcggctggcg cgcatggttg cccacatcac ctatctctcg gatagccaga tgggcgaaaa
     7561 attcggccgt cagttgcgcc atggcgagca caagttcagc tttgacgtcg attttgaagt
     7621 cgagtcctac ctgcgctatc aaggcgacaa gtttgccagc ttcttcgacg ccaacacgta
     7681 tctgaacacc accaaggccc tcgactactt cgatcccgcc tacgcctacg atggcgacct
     7741 agccgcggcc ctggcgcggg ccaaagccaa gttctttgtc gccagtttct caacagactg
     7801 gcgtttttct cccgcgcgtt cgcgtgaaat tgtttttgcc cttctgcata acagacggca
     7861 tgttgcctac gccgaaatcg atagcgacgc ggggcacgat tcctttctgc tggatgacgc
     7921 ctattatcac gccgtattgc gcgcctacct ggagaacata tcagtatgag caatggaaac
     7981 ccgctgacgg ccatcgagcg tgagcgcttc gattttgccg tcatcgccaa ctggattccg
     8041 cccggagagc gcgtacttga cctgggttgt ggtgacggaa agttgctgcg ctatcttcgc
     8101 gaaagcaaag gcgtcgtcgg ttacggcgtc gaaatcagtc acgaaagcgt gctcggctgc
     8161 atccgcaacg gcgtcgacgt gatccagatc gacattgaag gcggcctttc cggcttcgag
     8221 aaccggtcgt tcaatcacgc catcatctcc caagcgctcc aggcaatgca cgctaccgag
     8281 cgaattcttg gcgaaatgct gcgcgtcgct gaggaagccg tcgtgagttt tcccaacttt
     8341 gcctatcgcg ccaaccggga ggcgattgca caaggccata tgcccgtttc agagaacctg
     8401 ccgttcgaat ggtacgagac acccaacgtg cgcttcttca caattgccga cttcgacgca
     8461 ctatgcgaca aactcggcat cgagatccgc gaacggctgg ctttcgacga tgccggacag
     8521 ccggtgactg acgacccgaa tctgaacggc agtctggcct tctatcgcct gggtcgaaag
     8581 gcttgatgcc agcctggctg agtgcgctgc tgacccgcaa gatgctgatc tgcgtcttca
     8641 ccgggttcag ctccggtctg ccgctctatc tgctactgaa cctcttaccg gcctggttgc
     8701 gtagcgaggg cgtcaatctc aaaaccatcg gtattttcgc gctgatccag ttcccctata
     8761 cctggaagtt tctctggtcg cctttcctgg atcgctatgc cctgcccgtc cttggtcggc
     8821 ggcgcagctg gtcgctgatc atgcagatcg gcctgctgct caccattggc ctgctcggcg
     8881 ggctgtcgcc aagcacagac atcgtcccgg ttctctggct taccaccttt ctcgcacttt
     8941 tctccgccac ccaggacatc gccctggacg cctaccgtcg cgaactgctg acggagaacg
     9001 aactgggatt gggcaatgcc gtacatgtga atgcctaccg tgtcgccggg ctggtgcccg
     9061 gttccttgtc attgattctc gccgacctgt tgccgtggtc acaggtattc tggatcaccg
     9121 ccgccttcat gctgcccgga atggtcatga tcctgctggt ccaggaaccg accaacgcct
     9181 atgccaggcc aaagaccctg cgtgaagccg ttgtcgagcc cttccacgaa tttgtgggtc
     9241 gctctggtct gcatggtgcc ttgttagtgc ttgccttcat ttttctctac aaacttggcg
     9301 attcgctgtg caccgcactg gcgacaccct tttacctcga tatgggcttc acaaaaacgg
     9361 atatcggtct gatcgccaaa catgccgggc tatggccggc ggtctttggc ggccttctcg
     9421 gcggcctgtg gatggtgcgt ctgggcatca atcgcgcgct gtggctgttc ggtgttgtgc
     9481 aactgctatc gattcttggt tttgcctggc tggcctggct gggaccgcag acggacattg
     9541 gcgttgacca acgcctggcg ctcgcaaccg tcattggctt cgaggcgctt ggcgttggcc
     9601 tgggcactgt ggctttcgtc gcctttatag cccgttcaac acaccccgcc tataccgcaa
     9661 cccaatatgc gttgttcacc agccttgctg ccgtgccgcg cacctttttc aacgcttcgg
     9721 cgggttggct cgtggaaagc ctgggctggt acagcttctt cctgctgtgc actgcgctgg
     9781 cgattcccgg catgctgctg cttcctcggg tggccccgtg gaagatagtg ccggtcaaag
     9841 gagcgcaagt tggcgtctga ggcaagcgcc tcaagcgccg agctgctgat cgccgccgct
     9901 cgtgagctgt cggccagggt tgaaaaactt acctttgctc taccagtttc acatatctat
     9961 aatccgctgg cttacgcttg ggaggcacac gagctctatt tgcgccgcta tggcgcgtgc
    10021 cgcaaaaaag tggttttcat cggtatgaat cccggaccgt tcggcatggt tcaatgcggt
    10081 gttccctttg gcgagattgc tgccgtacgc gactggatgg gaatcagaac gcaaattgac
    10141 aaaccggcaa tggagaaccc gaaacgccgg attgaaggat tttcctgtac gcgctccgag
    10201 gtcagtggtc gacgtctatg gggactgttc caacagcgct tcggcaaggc agaagctttc
    10261 ttcgccaacc actttgttgc caactattgt cccttggcct ttttcgacag cggccgaaac
    10321 attacgccgg acaaacttcc ttcagccgag aaggagccgc tcaatgcggc ctgtgatcga
    10381 catctcagac aactcgtgac agcgctcgaa gcgcagtggg tgatcggggt cggcggcttt
    10441 gccgagaaac gcgccaccga ggctttgaca ggtatggacg taagcatcgg gaaagtgctg
    10501 catcccagcc cagccagccc ggcagccaac aggggttggg ctgaagcggc gacacggcaa
    10561 ctgatcgccc agggagtctg gcactaaccc agcagcaata tcctggccgc gacgaccccg
    10621 gaaagcgtga gacgataccc gtatccctag ggtagagtct ctgaaaccgg gcgcgccgca
    10681 cgccgtacgc gccgacggtc aagccaccaa gcgtaaactg gcagcaacag tgcggaacca
    10741 ggcaggagca gaaacaatag atacggtgaa acgcgcgata gaactcgcaa ctggtccaag
    10801 agttcggact tttcattggc tgtccatttg tcgccgttgc gatttttcat cagcaagggc
    10861 atcaatccgc gcatgcgcac gacgtcagcg acaaatcctc gcgcctgccg tttctgggag
    10921 gccaccatgc gcgaaactac actggctttt gccaattctg tctcgcccct ttgctcaacg
    10981 ctcacaaacc ggctttcaga ccaaatatcg gcaatttgtc acgcagtgtc cgccaggttt
    11041 gccaagtgaa gaaagcacgt tttgcccagc gcacgatgcg ccccggcttc aagacgacca
    11101 gcactgcaac agcgacggta actatgctgg gatgttgttt gatgaagacg acaccactgc
    11161 gcacgccggc cacaatgcga tccgtcgtat acagtgcggc tcgcaaaggc ggcatctcct
    11221 gacccaacgc agcacgttgg gtggcgatgc gttcgagcaa tcggccacgc cgaataagca
    11281 gttcattcag ccgctgattc atggccaacc gcctctttca actggcgcag atcttccttg
    11341 agctcggcaa tactggttgc aaagacttgc tcgggccgct gcagcgagcg cttgaacaga
    11401 gcgaaaaaaa ccccactaag aactaggaac agcccagttg acacactcaa gacaagcagc
    11461 cggttgtccc agaacaatgc agtaagcaag gccacaatga gcagcgtaca gactccgaaa
    11521 cagaacacca ttccctgcgc catcagaatc agatgaatgg cacgcaactt ctcagcttcg
    11581 agttcgttgc cgagaagttc aagccgcgtc ttcccgctcg caagcagcgt gacgacgatg
    11641 cttttcaggg catggaataa accgctgttc cctctgcctg acctgtcgct catagcgaag
    11701 ttctgcctaa cgacggccga taatgatacc gaacaggaaa ccgatgcccg ctgcgacgcc
    11761 gacagcctgc caagggttct gattgacata gtcgtcggta gctcgcgccg ccgccttggt
    11821 cttatcgacc agcagcgcct cggcgtcagc gatacgcagc ttggcgtcgc gcaaacgctc
    11881 accgacccgc tcacgtagat cggccatttt ttcaccagcg acacctgcag tcgcacgaag
    11941 tatttcctcg gcatcggcga cgacactctt catatccgag acgaatttct gtttgctatt
    12001 gttggacatt tcgttaactt cagtcatggg acacctcatg tgataaggga aaagggaaat
    12061 ggcacgaaac gatgggagtg ctcgtgaacc acaaccctca gggtatctaa tttaaagcca
    12121 cttatcaatg cgctgagtca cagcgtgatt cacagcacat agtcatagtc gatctgtaac
    12181 ggcgcgtggt cggaaaaacg ctgggccttg tatacagcag cagcatgggc ggtgcgagcc
    12241 aaagctggcg aggccagttg atagtcgagc cgccagccaa cgttgttggc ccaggcctgc
    12301 ccgcggttcg accaccacgt gtagcactcg tcagtgcttg ttgcgtgtag ctggcgatac
    12361 acgtcgcacc agttaccctc gttcagcact tggctgaccc aggcccgttc ctccggcaga
    12421 aaaccggaat tcttctggtt gcttttccag ttcttgagat cgatttcctg gtgggcaatg
    12481 ttccagtcgc cgcacaccac gacatcgcgg ccactcttcg ccagatcaag gaggtgcgga
    12541 tgaaacacgt ccatgaaact gaacttcgcc gcttgacgtt ccggcgaact cgaaccggat
    12601 ggcagataaa gcgaaatcac ggagagcggg ccgaaatcgg cgcgcaaata gcgcccctcc
    12661 gcatcgaatt ctgcgtgacc gaaaccttcg gtgacggcat caggcttccg ccgacaccac
    12721 aacccgacgc cgctatagcc cttcttttcg gcgcagtggt agtacgcgtg caaaccggcg
    12781 ggcgccttca tctcgtcaga gagatcggca aactgtgctt tcaattcctg cagacaaacg
    12841 acgtcagcgt tctgagcgga caaccatggc aaaactccct tgcgccacgc ggaacgaatg
    12901 ccgttgaggt ttaggttaat gatgcgtaac atgtcacttc cgcgcccgag gctttggcta
    12961 gtacacaatg gactttcgtc aggaattcat cgagtttgcc ctggaacagg acgttctgcg
    13021 ttttggcgag ttcaagacca aggcggggcg gctgtcgccc tacttcttta atgccggttt
    13081 gttcaatgat ggtgcggcgc ttgaccgact gtcgcaattc tacgctaaag caatcctcgc
    13141 cagcggcctt gcagtcgata ccctgttcgg gcctgcctat aaaggcattc ctcttgttgc
    13201 cgtgagtgct ctggcactgg cacgcgcggg tcgcaacctg cccttttctt tcaatcgcaa
    13261 ggaagccaaa gatcacggcg agggcggtag catcatcggc gccccgctgg ccggacgtgt
    13321 gctgatcatt gacgatgtca tttctgccgg cacctcagtg cgcgaatcgg tcgagttgat
    13381 ccgtgcagcg ggcgcaacac cgtgcggcgt ggtcatcgca ctcgatcgca tggaacgcgg
    13441 cagagggcac ttgtcggcga cgcaggaagt ggagcgggat ttcgcaatcc cggtggtggc
    13501 ggtggccaca ctcgacgatt tgatggcatt tctccgtgag cgctccgact tcaaggagca
    13561 tcagtccgca gtaacacttt atcgccaaga gtatggtatt gcatccgctc attaagctag
    13621 gcgtattggc tattgcgctt gcgctcagtg gttcgggcat ggccgcaggg aaactctatt
    13681 gctgcgatga tgcaaagggc atgcaagtgt gcggcgatct gctgccacac gcatgttacg
    13741 gacgagctta tcgtgagatt ggcgcatccg gtcgtactct acgtaacgtg gctgctccac
    13801 tgaccgccga agagcgcgcc cagcaagccc ttgaagaggc gaaacgcaaa gaagaagaag
    13861 cggcactcaa ggaacaacag cgcaaggatc aggcactgct caacacttac ggtagcgtga
    13921 aagacattga agcaatgcgt tcgcgcgccc aggacgatgt tcagaggtca atcgcagccg
    13981 ctcaggagaa gattgccgaa attcgtgcgc agcgcaagca atacgaggac gaagccgagt
    14041 tctacaagaa gaagcagttg ccggctgcag ttcagaaggg actaaacgac acggctttcg
    14101 agataacggc gcaggaatcg atcatcgagt ccaagcaaaa ggaactcaag atcattcgcg
    14161 agaaatacga tgaggacaaa cgacggtaca tcgatttgag ccgccgtgga ctgacgcgac
    14221 aatagagaca gcgagcctgt tactccggca gaccgtttga aattttactc tccgagtttt
    14281 tttcgcaaca gttcgttaac ctgttgcggg ttggccttgc ccttggtcgc tttcatcgct
    14341 tggccgacca gcgcattgaa ggctttttcc ttaccggcct tgaactcggc gaccatcgat
    14401 gcattggctg ccagtacccc gttgatcagc acctcgattg cactgctatc ggtaatctgc
    14461 ttcaaaccgt gctgttcgat gatgtcatcg gctgttttgc cttcgtcgct ccacaggctt
    14521 tcgaacactt tcttggctat gttgtttgat accgttttgt cggcgatgcg cgccaccaat
    14581 ccgccaagct ggtggggcga gacgggtgat tcgccgattt cgcggccttc cttgttaaga
    14641 cgcgcagcga ggtcgaccgt cacccagttg gcgcaaattt tggcgctgcc gataccgcca
    14701 acggccacgg cttcctcgaa ataaccggcc acttcgcgcg acgcggtgag cgtcgttgca
    14761 tcataggaag acagcgagta ctgggcgaca aagcggtcgc gtttggcgac cggcaattcc
    14821 ggcatgctcg cccgcgttcg ctcgacccaa tcgcgatcaa taagcagcgg cagcagatcg
    14881 ggatcgggaa aatagcgata gtcatgtgcg tcttccttag agcgcatcgc actagtcgcg
    14941 ccagttccgg cgtcaaacag cacggtggcc tgccggatcg aacggccctc ctcgatttcg
    15001 gcgatctgcc actgcacctc gtagtcgatg gcctgctgca tgaagcggaa ggagttgaga
    15061 ttctttatct cgcgccgggt tcctagcttc tgctgcccga ccggtcgaac cgagacgttt
    15121 gcatcgcagc ggaaggaccc ttcctgcata ttcccgtcac agataccgat ccagcgaacc
    15181 aatgcgtgca gcgccttggc gtaggccaca gcttcggctg aggaccgcat gtcgggttcg
    15241 ctgacgattt caagcaaagg cgttccggca cggttgaggt caattcccga tttgccatgg
    15301 aaatcctcgt gcagcgattt gccggcatcc tcttccagat gcgcgcgtgt cagggtgatg
    15361 accttctcag agaggctgtc tccctggccc agttgaatcg ccagtctgcc gccaacgatt
    15421 accggcaatt catactggct gatctgatag cccttgggta gatccgggta gaagtaattc
    15481 ttgcgagcga aaaccgattt cgctgcaacc tccccatcaa cggccagccc gaatcgaatg
    15541 gcgcattcga cagcaccctt gttcagtacc ggcaacactc cggggagcgc aatgtcaaca
    15601 gcattggctt gcacattggg ggccgcacca aaatccgtcg aactgccgga aaaaattttc
    15661 gagttggtcg cgagttgcgc atgtgtctcg atgccaatta caacttccca tttgctcatc
    15721 gtttcgtgct cacctgtcaa gtacaacggc cattccgagt gtcaaccaga atcggccaga
    15781 gataactgaa agcgtctcca ccacactttg actggcaggc gttgaaagcg accgtaacgc
    15841 ccttttcctg ctcccacata cggacgacgc agccgctcgc gccatcagtc aataccacat
    15901 tgggaaagct ctcggtctgc ttgaaatact tcatatcgaa atggcatacg ccctgcttgg
    15961 gaatgctcac ctcggctacg aaacgtttga cttcggcctt cgtcacctgc aaatccattg
    16021 ctccccgccc gccggccaca tcgcggaaac gacacttggt gcgcacgtca agcgccttgt
    16081 cgggcatggg tttgagcgta cggcccaaca agtgcttcag cggctgcgac ctgatttttt
    16141 ctgttggttg agaagcgcca gccgctacct gcccggacga atcaggcgat gccgtctcgg
    16201 gggtagcaca ggctccgaga agcaaagcaa gcagcgtggc gaatatcaac ctcatcaaag
    16261 tcagtcaaaa cctgatggat gccgcaaatg ccagtcggtt gtctgctgat atcggtgcgc
    16321 gacattgagc agctttgctt cgctgaaata gtcgccgatc agttgcagac ccgcaggcag
    16381 gctgctggca aaaccacacg gaatcgacat tcccggcagt ccggcgaggt tgacggcaat
    16441 ggtatagatg tccgagagat acatctggac cggatcagcc gctttttcgc caagattgaa
    16501 cgcagtgctc gggctggtgg gtcccatgat gacatcgcac tgccggaagg cagccacgaa
    16561 atcgttggcg atcaaacgac gtacccgctg cgcctgcagg tagtaggcat cgtaatagcc
    16621 gtgcgagagc acgtaggtac cgatcagaat acggcgcttg acttcgacgc caaagccctg
    16681 ggcccggctc ctggcgtaca tttcgttcaa atcgctgtac tcgggggcgc ggtagccata
    16741 gcgaacgccg tcgaagcgcg agagattcga gctggcctcg gccggcgcga tcacgtaata
    16801 ggcgggcacc gaaagcttca tgttcggcaa gctgatgtcg accgtttctg cacccagctt
    16861 gcggtattcg gcgatcgccg cgtcaaccag ttgcatgact tcaggactgc aacccgatct
    16921 ttcgctggtg ccgaaaaact ccttgggcaa accaattttc agtccggcca gcggcttgtc
    16981 gccagcggcg gcggatatct cgcggccata gtcttctttt tcgcgactga ggctggtgga
    17041 atcgcgttcg tcaaagccag ccatggcatt gagcagcagc gcgcagtctt cggccgagcg
    17101 tcccatcggg ccggcctggt cgagcgaaga agcgaaggcg accatgccgt agcgagaaac
    17161 cacgccgtag gtcggcttca atccggtcag attgcacaag gccgccggct ggcggatgga
    17221 acccccggtg tcggtaccgg tcgccgcagg ggccatcagg gcggccacag ccgctgccga
    17281 accccccgac gaaccaccag ggacgcgtgc cgtatcccag gggtttttca ccgcaccaaa
    17341 gtaggacgtt tcgttcgagg agcccatggc gaactcgtcc atgttcgttt tgccaagaat
    17401 caccgcaccg gcggcgttgt aattttcgat aacggttgca tcgtaagggc taacgaaatt
    17461 gccgagcatc ctcgaaccac atgtggtcag ccagcccttt gtgcaaaaga tgtccttctg
    17521 cgcgagcgga ataccagtca gcggtccggc gcccttgcca gctttcccga cggcacgcga
    17581 ttcgtcggcc gctcttgcct gcgccaggct cttgtcggcg tcaacggtga caaacgcgtt
    17641 gatttcagga ttcaggcggg caatccggtc gagatagagt tgtgtcagct cgacgctgga
    17701 aatctccttg gccgccaatg cctgcgacaa cgccgaaacg ttgcgggcga tcatcgcggt
    17761 cattcgagca ccttcggcac gaggtacagg ccggcctcga cctcgggggc aatggcctga
    17821 aaggcatcgc ggcaatcttc ttcggtgacg cggtcgctac gcaagcgctg agcgagatcc
    17881 tgagcatgcg ccatgggctc aacgccccgg gtgtcgacgg cctgcatctc ttcgatcagg
    17941 ccgaatattc cgttcaggtg accgagcgtg gtttccgctt cggcatcgct gacttcgatc
    18001 cgggcgaggt gcgcgacgcg ccgaacttgt tccagggtga gcgacatggc tatgagatcg
    18061 gtaaggtgtt aaaacaaaga gccatataag gtatcataaa tgctttgctg accgcagccc
    18121 ctgcggagct tttacggata ttcaggcatg ttcagttttc tacg
//
LOCUS       DAORPM010000014        18009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_572_length_18009_cov_20.752479, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000014 DAORPM010000000
VERSION     DAORPM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 18009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18009
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_572_length_18009_cov_20.752479"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4898)
                     /locus_tag="PLD53_01420"
     CDS             complement(<1..4898)
                     /locus_tag="PLD53_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRC59594.1"
                     /translation="MRLKRIAEAVMWLPGFAPEDDPIAVDGFAGQEWKGEIIAFPSKE
                     ARQSSVMTFWPGLNASDFDGLSGEVTKFEANCSAIVLLRKLEDEGRGPTTEERTILNR
                     YTGWGGLPWAFNPDQDDSNRKIRVERLQTLLAEDEYESARASTNSAHYTSVEIIEAMW
                     AAVRRLGFTGGRILEPSAGNGYFLGAMPREVAEKSTVTAVEIDQLSARLLTSLYGDHG
                     VRVANEGFEVARLPADFFDLVISNVPFGNYQTPETRHVPFRDFLIHDYFFARALEVVR
                     PGGLVAFITSAGTLDKSDTAARKYLANRARLLGSIRLPLQAFQKIANTEVTTDIVFLQ
                     KLKEGEAGNGDAWAEWPVEIPSSHIARDAGIPAWQWRNVRVCKWFAERPEWIVGKLTL
                     AENGYGKANACTFEGDIGAALLERVALLPEGVYRAVEQTRDRSEGCAEVRVLAGNFVK
                     PGAYKLVDGKVAVSEGHELRVVEGRLSARAVERIRGMIEIRDAARKLVAAQASTDDDT
                     LLAIYRVVLNAAYDAFSAKCGHIHAKANRLAFRGDPDLPLLLSLERWDEETQTAEKMD
                     IFHRRTVGAARQIEHCETPEEALIASIQQTGRVSVARMEALLGQPGYEFIPQLQEVGA
                     IFLNPDSGSWETADTYLAGNVRQKLMFAENAGRKFASNVEALKAVIPDDLAPHEIGAR
                     IGSVWIPATDYQAFLDEIMECEGNRVEFNATAGAWQIDPNHAARWSVAATQTYGTERV
                     GAMTLFEQALNQQVPTVTDPAPDDRSKRIVNQKETIAAREKLQELKGKFQEWLWLDDE
                     RTQRLVRLYNDQFNCIVQRRYDGKHLVLPGFSQVYRLHDHQRDAIWRVVSCKSNTLLA
                     HVVGAGKTLTMICAGMELRRLGVAGKPCYVVPNHMLEQFAAEFLRAYPGANILMASKD
                     DLQGDKRRTLLSRIATGDWDGVLITHSSFERIKLGDGFIEDFIQKQIDEIEQAIRDAN
                     QNRGNRIVKELERAKKVWLARLTKLAAREKKDDVLTFDELGIDWLFVDEAHLFKNLYR
                     FTKMTRVAGLPNSNSERAFDMFLKTRCVMARHQGRAGVAFATGTPVSNSMAEMWTMQR
                     YLQPDTLSAFRLQQFDTWAGNFGESVTALELAPDGRGYRMHTRFARFVNLPELMSMFG
                     QVADIRTAEMLKLPVPRALKETATAKPTPELKAYVQTLVERSEAIRNGTVTPQQDNML
                     AVTGDGRKAALDMRLVDPFAEDYEGSKTNQCAEKVFGVWKETASTRGTQIVFCDLSTP
                     VEDGRFSVYQDLRRKLIEMGVPVADIAFIHDYDSDSQKEELFKAVREGRVRVVLGSTQ
                     KMGVGTNVQTRLAALHHLDAPWRPSDVEQREGRIIRQGNLNEEVRIHRYVTEGSFDAY
                     IWQTLETKARFIAQVMQGNTGMRSAEDVELAALSYAEVKALASGNPLVLEKAGVDTEL
                     AKLSLLKSQWEQQQWRNKSELAALPGRIECIQQRIAGMEGDVANRQNVTGDSFRMQID
                     GTWYSDRTEVTRQLVLRFSVVKPGDTRVIGLFGGFRMAIRSHVKRDFGKRLVLLGNVE
                     HEAGHTDNPNGFIRVLENLMNRMEERLAEDSDYLARTEKRMSDLKAEIAKQFDKADRL
                     AWLAFRQKEIDAALDLSKGDTSA"
     gene            complement(5137..5487)
                     /locus_tag="PLD53_01425"
     CDS             complement(5137..5487)
                     /locus_tag="PLD53_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59595.1"
                     /translation="MGNRFDGYRRLTFQFNDGWKGEDAHEFIGEFLMLKCRVRPPGDQ
                     EACYDEAGERIFTVRAPRGVSRGDIINALQDVFTTACRCEHDCCGHLQTRAGLPRRIK
                     RREWVVEVRCFHNI"
     gene            complement(5490..5945)
                     /locus_tag="PLD53_01430"
     CDS             complement(5490..5945)
                     /locus_tag="PLD53_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59596.1"
                     /translation="MSQNIISVDSAEHATLLAALRYYQQNGQGEPSSRCDAIHAIATD
                     GDVQISLDTAGIDALCERINLCTPVRCVIGLEGGLVTGVTANVDLEFVVLDYDVEGCD
                     DDEVMTVPSLWGEDKVIDVYKRGFYDADVAPESVERLHAAIEAIMDAEA"
     gene            complement(6108..6302)
                     /locus_tag="PLD53_01435"
     CDS             complement(6108..6302)
                     /locus_tag="PLD53_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59597.1"
                     /translation="MKHIPTPGAYRELFKAGLIELLKGNRLSELQRKDAEHYLRLLED
                     PNYRFGILQSGGGYADFFRS"
     gene            complement(6299..6544)
                     /locus_tag="PLD53_01440"
     CDS             complement(6299..6544)
                     /locus_tag="PLD53_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59598.1"
                     /translation="MKLDTGVVPVDEQTYHAVATTRDPRTDEGMEGTPACDVVNQSRI
                     RAIQLARKWAGEGYWGSVYNQKTGECVIDYAPKGDGR"
     gene            complement(6614..6859)
                     /locus_tag="PLD53_01445"
     CDS             complement(6614..6859)
                     /locus_tag="PLD53_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59599.1"
                     /translation="MRTLLSTRRARFYRLVRQANEQSSQWLEDVLKDKTRNNGHFTAV
                     ARLACLRILVCRDYGINTPSGLPYPQRKKAVRAALGI"
     gene            complement(6864..7139)
                     /locus_tag="PLD53_01450"
     CDS             complement(6864..7139)
                     /locus_tag="PLD53_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59600.1"
                     /translation="MKGSFKPFRQELVQVVDLDERGWFKSHVENQNGRTVFSFSNEDE
                     KTGWPSEDGLWLVEDGFMRHGRDTGGLLEYLKNAGIAKPTATLRVEG"
     gene            complement(7136..7573)
                     /gene="radC"
                     /locus_tag="PLD53_01455"
                     /pseudo
     CDS             complement(7136..7573)
                     /gene="radC"
                     /locus_tag="PLD53_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            complement(7903..8724)
                     /locus_tag="PLD53_01460"
     CDS             complement(7903..8724)
                     /locus_tag="PLD53_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HRC59601.1"
                     /translation="MEKPDWNSLLQQALTLPGTLSKAYSVFHDYSLGNAILAAFQLAA
                     RGLPLAPIASFNRWKNLGRKVRKGEKALSLIMPVTVKRKDKDEHTQEEGTGGAYMIFL
                     MKRNWFSLDQTEGEALQPEVWSPEWDKSRALVVLDIREVPFEMIDGNCQGYAKRGVRE
                     VAVNPVAAMPWKTLFHEIAHHLLGHTATGDETGVMADGPTMPKCIKEAEAEATAYLCC
                     ATLGLPGMEESRGYIQSWLDGKEFPEKSARRVFAAADKILKAGTQAVKEVAMRDE"
     gene            complement(8711..8905)
                     /locus_tag="PLD53_01465"
     CDS             complement(8711..8905)
                     /locus_tag="PLD53_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59602.1"
                     /translation="MYHYEDGTTRWRRFNGRGDVRACRAPRGWQASADLIDVHPVTGK
                     YLGVSDWWIKEVKEAGHGKA"
     gene            complement(9138..9290)
                     /locus_tag="PLD53_01470"
     CDS             complement(9138..9290)
                     /locus_tag="PLD53_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59603.1"
                     /translation="MKYQYLGPQTVAELADGKRVLLFKGSVLLVLILSAQLKALVEKG
                     LLKEIA"
     gene            complement(9393..10469)
                     /locus_tag="PLD53_01475"
     CDS             complement(9393..10469)
                     /locus_tag="PLD53_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRC59604.1"
                     /translation="MEAKEVKAAAHGRWGGILSALAPQLQPALDRVGHHVSCPVHGGK
                     DGYRVFNDVDDSGGGVCNTCGVHPDGFATLMWVSGMDFKEALKSVAEYLRVGIIARPF
                     GGRTISRQTRKGEDDEKARQALNRVWNESIALSHSDAEPARLYLARRGISIRQPETLR
                     LHPSLAYFEDRKEIGTFPAMLAMVQGHGGNAITIHRTYLTADGYKAPVESPRKLMRYP
                     SQRKLAGGVIRLVEAGAVLAVAEGLETALAVMEGTKMPVWCAVNAYLLEHFYPPPGVS
                     QILVFADKDRPSAQHPRGHGQESARRLVQRLWERGIKASAIVPAGEIPAGQRSLDWLD
                     ILNRDGKAGFPSLQSVEHAMRRVA"
     gene            complement(10648..11055)
                     /gene="ssb"
                     /locus_tag="PLD53_01480"
     CDS             complement(10648..11055)
                     /gene="ssb"
                     /locus_tag="PLD53_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRC59605.1"
                     /translation="MLNKVQLIGFLGADPELRRSNDSDAIARANIATSESWKKDGEKH
                     EKTEWHRVVFFGKLAEIVGEYLKKGSLVYVEGRLQTRSWDKDGETRYTTEIVGESMKM
                     LPSGGKAREGSDASRSAPKGNSRAAQPFDDGPF"
     gene            complement(11142..11798)
                     /locus_tag="PLD53_01485"
     CDS             complement(11142..11798)
                     /locus_tag="PLD53_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59606.1"
                     /translation="MDVVINHEQELFVVSAGKGYSTLGFEHVFKQLKQLVARLNLSIQ
                     IREDEKGTIGQYADFQRAINEAAKANLKETWFNLDTPVKVRRILEQYRKSGKAIRLFY
                     GDVERGEDWLEENDVVGKIGRSSGTFKIPILLQNGEAWGVSILDHAIVRLMDVADRKV
                     LWEHPKYQAPQLQIVAKRVGEDPYSVFVDGGPHASFRTLAKAAQWVAFMAGECMEAPQ
                     "
     gene            complement(12039..13166)
                     /locus_tag="PLD53_01490"
     CDS             complement(12039..13166)
                     /locus_tag="PLD53_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HRC59607.1"
                     /translation="MSLQALKNINRTTPTDAEQPKGFPAMLEQFKGEIARALPKHINP
                     DRMARIALTAFRMTPKLGECDPRSVFAAVIQSSQMGLEVGLMGEAHLVPFGNQCQLIP
                     GYTGLMKLARQSGLVQDIYAHEVRVNDNFTLKLGMERSLDHEPLTTLGGFPAADDERG
                     EVAGFYSVAVFKDGSRTFVAMGRKEVEKIRDNSRGYQSAKRYKKESVWDSDFTAMGLK
                     TAIRRLCKFLPKSPELASALALDAAAEQGKAQNLNLTDVIEGNYAPIIDDETGEIADA
                     KPVTSRDARQSRPGGDPVEVADATGKKVKVDGVSAVASDPSDQEPSARLKAIYEQMAK
                     ATSTDVLDEIYVRAEGDLDGPELEALMREYRTCKAAIGRLI"
     gene            complement(13300..13548)
                     /locus_tag="PLD53_01495"
     CDS             complement(13300..13548)
                     /locus_tag="PLD53_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59608.1"
                     /translation="MPSNPIEKVAPYLYEVGDPILSIAARVEAKVDALKQAEAEVVRC
                     RRALKESRRLLLACVKHSYVDSEIETADAMCNRQKFRS"
     gene            complement(13550..14503)
                     /locus_tag="PLD53_01500"
     CDS             complement(13550..14503)
                     /locus_tag="PLD53_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HRC59609.1"
                     /translation="MLIETQVGLLSMAAEEYHRNKDAVGHSSLVRIMRSPAHFQEYVT
                     HPPEPTPAMAFGTAVHSAVLEPAAFASDYAVFDESQLEGSLQSLDDYKAAAAELGIKV
                     GKMKKEEIKDAIKVADVESRFVFREDAIARLYDGKQILTPTLMMAIRSIQASLSRHTG
                     ASRLLANGMAEMSGFWSDPETGLGCKCRPDWLVMADETITGIVDVKTCTDASAEGFAR
                     SIATLGYDLQAAFYQDGIKAITGRTLPFYFIAVEKDAPQAAAVYRASEDVIDVGRAKY
                     RGALQLLQWCRANNRWPAYQPNGEIETIELPRWAANFDLES"
     gene            complement(15237..15500)
                     /locus_tag="PLD53_01505"
     CDS             complement(15237..15500)
                     /locus_tag="PLD53_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59610.1"
                     /translation="MATPEHWPGAQKIPEWHRSVSASSLVSHDDGEEDWSKYDVPAFI
                     RRGIPMPKLEVVQPKKPAKRKKAVKKSQVVAPTETEARFEVVL"
     gene            15887..16525
                     /locus_tag="PLD53_01510"
     CDS             15887..16525
                     /locus_tag="PLD53_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59611.1"
                     /translation="MATRPPAFIPSASHDISRPYFTQKVRLHSLHAQQVFDRGFELCA
                     NAIFSLSVVLRVIGTDEQAREVETIVDDRLDKMFEDMRSEVTRLEKLADANGIEFAGV
                     TYSNPKELEARITSPRAVRYIGLIREFDGLVGKFDTLWLSGSIPDGNYSSSLYEWKRR
                     LLRIAGGFRGLASRASIAARKNATRVAETDSATPAIAVTPTETESPVSVLAV"
     gene            16526..>18009
                     /locus_tag="PLD53_01515"
     CDS             16526..>18009
                     /locus_tag="PLD53_01515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRC59612.1"
                     /translation="MDLNPHQERAVSTVGHCTVLACPGSGKTRVLSERAIRLLASHDK
                     GRLCAVTFTRDAAEELKARILPSCGADVARRLAVGTFHSIALSQLKRLPRGQKPRRLL
                     SDGERMAVLRRCIAQHPTEATFEDVVKNIDSAKARIRTPVFADPGIEAIYIAYQDTLA
                     SEGAMDFADIVLSAVQKISSGELLPLPVRWLLVDEAQDMDEVQVAWVKAHGLAGIEVT
                     LVGDDDQSLYAFRHAMGYAGLIEVTETLASTETTLPLNYRCAPNILGHAAKLIAHNKE
                     RAPKRIEAFRTSTGDIQIVRASDRWDEADIVADRIRMFGIDGEWGILGRTNSQLDPIE
                     ICLSGSGIPYRRTGGKRIWDRKLGSAFLGIQRSVLDGGWTGAANALAFAGVAPAYLNS
                     REVQGEGDCMQYLTRILELRGPEGDHHQRVAGLLAGFSSWTEQARKGRVELVVHGVSA
                     WLLPHCKNDQHVAMLNRLESSVAKLSGSLGQRLRFLTTADNKQKS"
BASE COUNT         3573 a         5587 c         4962 g         3887 t
ORIGIN      
        1 gccgacgtat cgcccttcga tagatccagg gcggcgtcga tttctttctg acggaaggcc
       61 agccatgcca gccgatccgc cttgtcaaac tgctttgcaa tctcagcctt caggtcgctc
      121 atccgcttct ccgtccgggc gaggtagtcg ctatcttctg cgagccgctc ttccattcgg
      181 ttcatcagat tttcgagaac acggatgaac ccgttggggt tgtccgtatg cccggcctca
      241 tgctccacgt tgccgaggag cacaagacgc ttgccaaagt cacgtttcac atgcgagcga
      301 atggccatcc ggaatccgcc aaacagcccg atcacccggg tgtcgcccgg cttcacgacg
      361 ctgaaccgca gcaccagttg cctggtgact tcggtacggt ccgaatacca cgtgccgtca
      421 atctgcatgc ggaagctgtc tcccgtcacg ttctgacggt tggcaacatc gccttccatg
      481 ccggcgatcc gttgctgaat acactcgatg cgaccaggca atgcggccag ctccgacttg
      541 tttctccatt gctgctgctc ccattgagat ttcaggagcg acagcttggc aagctcggta
      601 tccacacccg ccttttccag caccagggga ttccccgacg ccagtgcctt cacctcggcg
      661 tatgacaacg ccgccagctc cacatcctcc gcgctgcgca ttccggtgtt cccctgcatg
      721 acctgggcga tgaaacgcgc cttggtctca agggtctgcc atatgtacgc atcgaaagag
      781 ccttccgtga cgtagcggtg gatacgcact tcctcgttca ggttgccctg gcgaatgatg
      841 cgaccttccc gctgttccac gtcagatgga cgccacgggg cgtccaggtg atgaagcgcc
      901 gcaagacggg tttggacgtt ggtaccgacc cccatctttt gcgtgctgcc cagtaccacg
      961 cgcacacgtc cctctcgcac cgccttgaac agttcctcct tttgggagtc actgtcgtag
     1021 tcgtgaataa aagcaatatc tgcaaccggc actcccatct cgatgagctt ccttctcagg
     1081 tcctggtaga cgctgaatcg cccatcttca acgggggtgc tcaaatcaca aaatacgatc
     1141 tgtgtgcctc tggttgatgc ggtttccttc cagaccccga agaccttctc ggcgcactgg
     1201 ttggtcttgc ttccctcata gtcctcggcg aacggatcca ccagccgcat gtccagcgcg
     1261 gccttgcgac catcgcccgt caccgccagc atgttgtcct gctgaggcgt taccgttcca
     1321 ttcctgatcg cttccgaccg ctccacgagg gtttggacgt aggctttcag ttccggggtc
     1381 ggcttcgccg tggccgtctc cttcaatgcg cgaggtaccg ggagtttcaa catctccgcc
     1441 gtgcgtatgt ccgccacctg cccgaacatg gacatcagtt ctggaagatt gacgaaccgc
     1501 gcgaagcggg tatgcatccg gtagccccgg ccatcgggcg ccagttccag cgcggtcacc
     1561 gactcgccga agttgcctgc ccaggtatcg aactgctgaa gcctgaacgc cgacaaggtg
     1621 tcgggttgca gataccgctg catcgtccac atctccgcca tgctgttaga cacaggcgtt
     1681 ccagtggcaa acgctacacc cgcgcggcct tgatgcctgg ccatgacaca tcgcgttttt
     1741 agaaacatgt cgaaggcccg ctcggagttg ctgttgggca aacccgccac gcgcgtcatc
     1801 ttggtgaagc ggtagagatt cttgaacagg tgcgcctcat caacgaagag ccaatcaata
     1861 cccagctcgt cgaacgtcag cacgtcgtcc ttcttctccc tggccgcaag ctttgtcagc
     1921 ctcgccagcc agaccttctt cgcccgctca agctccttga cgatcctgtt gccacgattc
     1981 tggttcgcgt cacgaatggc ctgctcgatc tcatcgattt gcttctggat gaaatcctcg
     2041 atgaacccat cgccgagctt gatccgctcg aacgacgaat gcgttatgag cacgccatcc
     2101 cagtcgcccg tggcaattct ggacaaaagg gttctgcgtt tatccccttg cagatcgtcc
     2161 ttgctcgcca tgaggatgtt ggccccaggg taggcacgca ggaactcggc cgcgaactgc
     2221 tcaagcatgt gattgggcac gacgtagcag ggttttccgg cgacaccgag ccttctcaac
     2281 tccatgccgg cgcagatcat ggtgagggtt tttccggccc ccacgacatg cgccaggagc
     2341 gtattgctct tgcatgagac gactcgccag atggcatcac gctgatggtc atgcaaccga
     2401 tacacctgcg aaaagccagg gagcaccaga tgctttccgt catagcgacg ctgcacaatg
     2461 caattgaact ggtcgttgta aagccggacc agccgctgtg tgcgctcatc gtcaagccac
     2521 agccactcct ggaattttcc cttgagctcc tgcagcttct cccgtgcggc aatcgtttcc
     2581 ttctgattca cgatccgttt gctccgatca tccggcgctg gatcggtaac cgtcggtacc
     2641 tgctgattga gcgcctgctc aaacagcgtc atcgccccaa cccgctccgt accgtaggtc
     2701 tgcgttgctg ccacagacca ccgggcggcg tggttcgggt cgatctgcca tgcaccggcc
     2761 gtcgcattga actcgacgcg gtttccctcg cattccatga tttcatccag gaatgcctgg
     2821 tagtccgttg ccggtatcca gacagaccca atccgtgcac caatttcatg cggtgcaaga
     2881 tcatccggaa tgacagcctt taacgcttcc acgttggacg caaacttccg tcctgcattt
     2941 tcggcaaaca tcaacttctg gcggacgttg ccggcgaggt aggtgtccgc cgtctcccag
     3001 cttcccgaat ccgggttgag gaaaatggct cccacctctt gcagctgggg aatgaactcg
     3061 taccctggtt gcccaagtaa cgcttccatc ctcgccacac tgaccctccc cgtctgctgg
     3121 atagaggcaa tcagcgcttc ctcgggcgtt tcacaatgct cgatctggcg cgcggcccct
     3181 accgttcgac gatggaagat gtccatcttt tcggcggttt gagtttcctc atcccaccgt
     3241 tccagggaca acagcaacgg gaggtccggg tcgccccgaa acgccagcct gttggctttg
     3301 gcgtggatat ggccgcactt tgccgaaaag gcatcgtagg ccgcattcaa caccacacgg
     3361 tagatcgcca gcagagtatc gtcgtccgtg ctggcctgtg ctgccaccaa cttgcgggcc
     3421 gcatcacgaa tctcgatcat gcccctgatc cgttcaaccg ccctggccga caacctgcct
     3481 tcaaccaccc gcaactcgtg gccttcagat accgccacct tgccatccac cagcttgtaa
     3541 gcacccggtt tgacaaagtt cccggcgagc acgcggactt ctgcgcagcc ctccgaacga
     3601 tccctcgttt gctctaccgc tcgatagacc ccttcaggca acagagcgac gcgctcaagc
     3661 agcgccgcgc cgatgtcacc ctcgaaggtg caggcattcg ccttgccgta gccgttctcg
     3721 gccagggtca gcttgcctac gatccattcc gggcgttccg caaaccactt gcacacgcgg
     3781 acgttacgcc actgccacgc gggaattccc gcgtctctgg caatatggct gctgggtatt
     3841 tccaccggcc actctgccca ggcgtcaccg ttgccggcct cgccctcctt caatttctga
     3901 agaaagacga tatcggttgt gacttccgta ttggcgatct tctggaatgc ctgaagcgga
     3961 agacgaatgg atccgagcaa tcgcgcacgg ttcgccagat acttcctggc ggccgtatcg
     4021 gatttgtcca gcgttccggc actcgtgatg aatgcaacga gacctcccgg cctgaccact
     4081 tcaagcgcac gcgcaaaaaa gtagtcgtgg atcagaaagt cccgaaacgg aacatgacgg
     4141 gtctccggtg tctggtaatt gccgaacgga acgttcgaga tcaccagatc gaagaagtcg
     4201 gccggtaacc tggccacctc gaacccctca ttcgccacgc gaaccccatg gtctccatac
     4261 agcgacgtga gcagtcgcgc agagagttgg tcgatctcga cggccgtgac ggtcgacttt
     4321 tcagctacct cccgaggcat ggccccgaga aagtagccat ttccggccga cggctccagg
     4381 atgcgaccac cggtaaaacc caggcggcgg accgccgccc acatggcctc gatgatttcc
     4441 accgacgtgt agtgggcgct gttggttgat gcgcgagcac tttcgtactc gtcctcggcc
     4501 agcaaggtct gaagtcgttc gacacggatc ttgcggttac tgtcatcctg atccggattg
     4561 aatgcccagg gaaggccgcc ccaaccggtg tagcgattga gaatggtccg ctcctcggtt
     4621 gtcggccccc tgccctcatc ctccagcttt cgcagaagga cgatggcgct gcagttggcc
     4681 tcgaacttgg tgacttctcc ggacaggcca tcgaagtcgg atgcattcaa ccctggccaa
     4741 aaagtcatga cgctcgactg tctggcctcc ttcgacggga aggcaataat ttcacctttc
     4801 cattcctggc cggcgaaccc atcaacagca atgggatcat cctcgggcgc gaagcctggc
     4861 agccacatga ctgcttcggc aattcttttg agtctcatga gtgtctccaa aagataaaac
     4921 ccctggagac acgtctcccc ttgcggggat gggtgcccgc aagggttggt taaagtcgtc
     4981 acggtgttcc gtgacaaccg gctgattgct gtttgtttca agtcgccagc tgcgaccttt
     5041 caccccatcg gattacccga caggaagaat tcggcttgcc ggggcgtact catcgagtgc
     5101 gtccgggtaa gccccgtgcc gaggcacggg ggttggtcag atgttgtgaa agcagcgaac
     5161 ctcgacgacc cactcgcgac gcttgatgcg cctcggcagg ccggcacggg tttgcaggtg
     5221 cccacaacag tcatgctcgc aacgacaggc tgtcgtgaat acatcctgaa gcgcattgat
     5281 gatgtcgccc ctggatacgc cacgcggggc gcgcaccgtg aagatgcgct ccccggcttc
     5341 gtcgtaacaa gcctcctggt caccgggtgg gcgaacacgg cacttgagca tcaggaattc
     5401 cccgatgaac tcatgcgcgt cttccccttt ccagccatcg ttgaattgaa acgtgaggcg
     5461 gcgatagcca tcaaaccggt ttcccatggc taggcctccg cgtccatgat cgcctcgatc
     5521 gcggcgtgaa gtcgctctac cgactcgggc gccacatccg catcgtagaa accacgctta
     5581 tagacgtcga tgaccttgtc ttctccccag agggaaggga ccgtcatgac ttcgtcgtca
     5641 tcacagcctt cgacgtcgta atcgagcacc acgaattcga ggtccacgtt cgccgtgacg
     5701 ccggttacca gacccccttc gagtccgatc acgcagcgca ccggcgtgca cagattgatg
     5761 cgctcgcaca gggcatcgat accggcggta tccagtgata tctggacatc gccatccgtc
     5821 gcaatggcgt gaatcgcatc gcaccgactg gacggttcgc cctggccgtt ctgctggtag
     5881 tagcgcagtg cggccaggag ggtggcatgt tcggcagaat cgaccgagat tatgttttgt
     5941 gacattgtca ggctccaata aaaaggcgga gccatcgccc cttgcggggg tgagtggccc
     6001 gcatgggttt gaaatactgc tgatggttgc ttcgttttca ggccatcgct ctcagcaatg
     6061 ccgatccggc aggcgatgca ttggccctgc aggaacaggc actcagccta gctcctgaaa
     6121 aagtccgcat acccgccacc gctctggaga atcccgaatc tgtaattggg atcctccaat
     6181 agccggaggt aatgttcagc atccttccgc tgaagctcgg acaaccggtt tcctttcaga
     6241 agctcgatca gccctgcctt gaacagttcc cggtatgcgc caggcgtcgg aatgtgtttc
     6301 atcgtccatc tcctttcgga gcgtagtcaa tgacgcactc tcccgtcttc tggttgtaga
     6361 ccgaccccca gtacccttca ccggcccatt tccgggcaag ctggatagcg cggatgcggc
     6421 tttgattgac gacatcgcaa gcgggagttc cctccatgcc ctcgtcggtt cgcggatcgc
     6481 gagttgtcgc gacggcatga taggtttgct cgtccaccgg aacgacgcct gtatcgagtt
     6541 tcattgttga cctccagaat ggtgagaggc cactccccaa ccgggaagtg gccccggttg
     6601 ggaaaggatt gcttcagatg ccgagcgccg cccgcacagc ctttttccgc tgcggatacg
     6661 gcaaccccga aggggtgttg atgccgtagt cccggcagac caggattcgc aggcaggcca
     6721 ggcgggcaac ggcggtgaaa tgcccgttgt tccgcgtctt gtccttgagc acgtcctcca
     6781 gccattgcga tgactgttcg ttggcttgtc ggacgagccg gtagaacctg gcgcggcgag
     6841 tggagagcaa agttctcatg ccatcagccc tccacgcgca gggttgcagt cggtttcgca
     6901 attccggcgt tcttcagata ctccagaagc ccgccggtat cgcgcccgtg ccgcatgaag
     6961 ccgtcctcga caagccacag cccgtcttca ctcggccagc cggttttctc gtcctcattc
     7021 gagaacgaaa acaccgttcg gccgttctga ttttcgacgt gcgacttgaa ccacccgcgc
     7081 tcgtcgagat cgaccacctg aaccagttcc tgccggaagg gcttgaagct gcctttcata
     7141 gcagccccct ttcggcgaac gaaagcgtct tgttcccggc cacgatgaag tggtccagaa
     7201 ccttgacatc caccagtgcc agcgcggcct tgaggctagt cgtcagcact tcgtccgccc
     7261 gcgactggtc ggcttctcca ctcggatggt tatgagcgag tattaccgct gccgcgttgt
     7321 gcaccagcgc tcgcttgacc acttcccgcg ggtagaccga ggtctgggtc agcgtgccgc
     7381 gaaagagttc ttccgcgacg atcaaccggt tcctggcgtc catccacagg cacatgaata
     7441 cttcatgctc gtaaccgccg atccggccac acaggaatgt cgaggccgca ctgggcgagg
     7501 ccagattgat ccccgtactg ctcatgtctt cctgcattgc ccggatgtaa agctccttcg
     7561 cagcggcgat ttgcggctgc gcctcgtagg acgtccgctc ttcccctgca atgaagcttg
     7621 tctgtcgtgc cgacctgatc ccgaaaattt ccgagagggg acgtgttgac agggcgcgac
     7681 ccgccgggcc acagagaacc gccagcaaat cggcattggt catttcaatg gtgcgcattt
     7741 ccatttcatt tgcctttcag gaaatggggc cgtgcgcatc cccagggggc gcatacggcc
     7801 cctgagggag taaatatgga agccgccgaa gccactccca tcttttagaa acgtgctgcc
     7861 tgccatccat gccagacctg aacatgggag gcgacacgcg aattactcat cgcgcatcgc
     7921 cacctctttc acggcctggg tacccgcctt gagaatcttg tccgccgccg caaacacccg
     7981 acgcgccgac ttttccggga attccttccc atcgagccag ctctggatgt agccccggct
     8041 ttcctccatg cctggcaacc cgagggtcgc gcagcacagg taggccgttg cctcggcttc
     8101 agcctccttg atgcatttcg gcatcgtggg gccgtcggcc atcactcccg tctcgtcacc
     8161 cgtggccgtg tggccaagca ggtgatgcgc aatttcgtgg aaaagtgtct tccatggcat
     8221 cgcggcaaca ggattgacag ccacctcgcg gacaccgcgt ttggcatatc cctggcagtt
     8281 gccgtcgatc atctcgaacg gaacttcccg aatatccagc acgacaagcg cccttgattt
     8341 atcccactcc ggcgaccaca cctccggctg gagagcctcg ccttccgtct ggtcgagact
     8401 gaaccagttg cgcttcatca aaaagatcat gtaagcgcca cccgtcccct cttcctgagt
     8461 gtgttcgtcc ttgtcctttc tcttgaccgt gaccggcatg atgagagaaa gggccttctc
     8521 gcctttcctg acctttcggc caagattttt ccagcgattg aagctggcaa tgggggcgag
     8581 aggaagacca cgcgccgcca gttgaaatgc cgcaaggatt gcgttgccga gcgagtagtc
     8641 gtgaaatacc gagtacgcct ttgacagcgt tccgggtagc gtcagtgcct gttgcagcag
     8701 ggagttccag tcaggctttt ccatgaccgg cctccttgac ttccttgatc caccaatccg
     8761 atacaccgag atactttccg gtcaccgggt gaacgtcgat caagtcggca ctggcttgcc
     8821 atccgcgcgg agcgcggcag gcacgaacat cgccgcgtcc attgaaccgc cgccagcgtg
     8881 tcgttccatc ttcatagtga tacatggttg cctccagacg aaaaaaccgc cgaaggcacc
     8941 aagcccgtcg ccggggtcaa tgccccggcg gggttgtgaa tgacgaccag ttgcctgagc
     9001 gtcgttgatt gctgattgtt tcgagcggcc agctaccgcc tatgacaccc ctggattacc
     9061 cgggagcgac aatttcgctt tccgcagcgc acctcgcgat gcgctctgga aagcccgcct
     9121 ttcggcgggc cctggtttca tgcgatttcc ttcagaagcc ccttctccac gagcgccttg
     9181 agttgtgcgc tgagtatcag gaccagaagc accgatccct tgaaaagaag cacccgtttc
     9241 ccgtccgcga gttctgcgac ggtttgtggt cccaggtact gatatttcat gatgccctcc
     9301 tgttacgagg gatctccgac ccaatgcatc cgggcagggt ttcaatatcg accgggctga
     9361 cgtcagcatt cctgtccatg gcgtcacctc cttcacgcaa cgcgacgcat ggcatgttca
     9421 acggattgca aggaaggaaa acctgccttg ccatcacgat tgagaatgtc gagccagtcc
     9481 agtgacctct gaccggcagg aatctcgccc gcaggaacaa tggcactggc cttgataccc
     9541 cgctcccaga gcctttggac cagccgtctg gctgattcct gaccatgccc ccttggatgt
     9601 tgcgccgagg gccggtcctt gtcggcgaag accaggatct gactcacgcc gggtggcggg
     9661 taaaagtgct ccagcagata cgcattgacc gcgcaccaga ccggcatctt cgtcccttcc
     9721 atgaccgcga gagccgtctc cagaccttct gccactgcca gcacggcgcc agcctccacc
     9781 agtcggatta ctccgcccgc cagcttccgc tgacttggat accgcatcag cttcctggga
     9841 gactcgacgg gggccttgta gccgtccgcc gtcaggtagg tacgatgaat ggtgatggcg
     9901 ttgccgccgt gaccctgcac catggccagc atcgccggaa acgtgccgat ctcctttctg
     9961 tcctcgaaat acgcgagcga tgggtggaga cggagtgttt ccggttgtcg gatcgagata
    10021 cctcttcggg ccagatacaa cctggccggt tcagcatcgg aatgagagag ggcgatcgac
    10081 tcgttccaga cccggttaag cgcctggcga gccttttcgt catcttcgcc cttcctggtc
    10141 tggcgagaaa tggttctccc gccaaacggc ctggcgatga tgccgacacg caggtactcg
    10201 gctacggact tcagtgcttc cttgaaatcc atgccggaca cccacatcag cgtggcaaat
    10261 ccatcgggat ggacgccaca ggtattgcag acgccgcctc ccgaatcatc cacgtcattg
    10321 aacacacggt atccgtcctt gccgccgtgc accgggcagg aaacgtgatg gccgactcga
    10381 tcaagtgcag gttgaagctg tggcgcaagg gccgagagaa tccctcccca gcggccatgt
    10441 gcagcagcct tgacttcctt tgcttccatg attgcctcca tgaacagccc cggacggggc
    10501 ctgccgaaat gcccgcgcga ggcacttggg aaggccccgt cgcgggccgg tgaattaccc
    10561 accggcatag aggcaccccg accggggtaa ctccccggtg gggttggtaa tgtcaggcgc
    10621 taaaaacagt gcctgagcgc gaagccatca gaatggcccg tcatcgaatg gctgagctgc
    10681 cctggagttt cctttcggcg ccgatctgga cgcgtcagaa ccttccctgg ccttgccgcc
    10741 gctggggagc atcttcatgc tctcgccgac gatttcagtg gtgtagcgag tctcgccatc
    10801 cttgtcccat gaacgggtct ggagacggcc ctcgacatac accagcgaac ctttcttcag
    10861 atactcgccc acaatctcgg cgagttttcc gaagaacacg acacggtgcc attccgtttt
    10921 ttcgtgcttc tcgccatcct tcttccagct ttcgctggtg gcgatatttg cgcgcgcaat
    10981 ggcatcgctg tcattgctgc gtcgaagctc ggggtcagcc cccagaaaac cgatcaattg
    11041 aaccttgtta agcatgatga actccttgtg atgaaaagga cggtcacacc atgcccctga
    11101 cgggagcatg acgatcccgc cgggttggtt gaaatactgg actactgcgg cgcttccatg
    11161 cactcaccgg ccatgaacgc cacccactgg gctgccttcg ccaaagttct gaagcttgcg
    11221 tgaggaccgc cgtcgacaaa caccgaatag gggtcttcgc caacgcgctt cgcgacaatc
    11281 tgcaactgag gcgcctgata tttcgggtgc tcccagagaa ccttccgatc ggccacatcc
    11341 atcagcctga cgatagcgtg atcgagaatg ctgacacccc atgcctcgcc gttttgaaga
    11401 agaatgggga tcttgaaagt acccgacgag cggccgattt tccccaccac gtcgttttct
    11461 tccagccagt cttcgcccct ctccacatct ccgtagaaga ggcggatcgc cttgccgctc
    11521 ttgcggtact gctccaggat tcggcgaacc ttgacaggag tgtcgagatt gaaccaggtc
    11581 tccttgagat ttgccttggc agcttcgttg atggcacgct gaaaatcagc gtactgaccg
    11641 atggttccct tctcatcctc gcgaatctgg atcgagagat tcagccgggc cacgagttgc
    11701 ttcaattgct tgaagacatg ctcgaagccc agggttgaat accccttgcc agcggaaaca
    11761 acgaacaact cctgctcgtg attgattaca acgtccattg caacctccag acgaaaaacc
    11821 gccggaggcg ctcgttccct gccgggagct tgcgccccac ggcggggttt taatggcaac
    11881 cggtttcccg attgcagcca gatgctgttt gtttcagaag gccagctacc ttcatgtccc
    11941 gttggcatag ccaggggaac aattttgaat aattcgcttt ccgcagcacg gaccgctcca
    12001 tgctctggaa agccccgtgc cgaagcacgg ggagggtttt agatcagtcg gccgatggct
    12061 gccttgcatg tgcggtattc ccgcatcaag gcctccagtt cgggtccatc cagatcacct
    12121 tcggcgcgga catagatctc gtccagcacg tcggtgctcg ttgccttggc catctgttcg
    12181 tagatcgctt tcagcctggc gctgggttcc tggtcggagg gatcggaagc gacggcagac
    12241 acgccatcca ctttcacctt ctttccagtt gcgtcagcca cctcaaccgg atcgccaccc
    12301 ggcctggatt gcctggcatc gcggcttgtc accggcttgg cgtctgcgat ctcgcctgtc
    12361 tcgtcgtcaa tgatcggtgc gtagttgccc tcgatcacat cggtcaaatt caggttttgc
    12421 gccttgccct gctccgcagc tgcgtccagg gcgagtgccg aggccagctc gggtgatttg
    12481 ggcaggaatt tgcacaggcg acgaatcgct gtcttcaggc ccatggccgt aaagtcggaa
    12541 tcccatacag actccttctt gtatcgcttg gcagactgat agccccgcga gttatcgcga
    12601 atcttctcaa cctccttgcg ccccatcgcc acgaacgtcc gacttccatc cttgaacacc
    12661 gcgacggaat agaaaccggc gacctcacca cgctcgtcgt cggcggcagg aaagccgccc
    12721 agcgtcgtca atggctcgtg atcgagactg cgttccatgc ccagcttgag ggtgaaattg
    12781 tcattcaccc tgacttcgtg ggcatagatg tcctgaacca gcccggactg tcgagcgagc
    12841 ttcatcaggc ccgtgtagcc ggggatcagc tggcactggt tgccaaacgg caccagatgc
    12901 gcttccccca tgagaccgac ttccagcccc atctgcgaag actggatgac cgcggcaaat
    12961 acgctgcggg gatcacactc tccaagcttg ggtgtcattc ggaatgcagt aagtgcaatg
    13021 cgcgccatgc ggtccggatt gatgtgcttg ggcagggcac gggcaatttc acccttgaac
    13081 tgctccagca tggccgggaa acctttcggt tgctcagcgt cggtgggcgt agtgcgattg
    13141 atgtttttca aggcttgcag tgacatggta ttgacctcca gaaaaatgga aaaggcggtc
    13201 acaccacgcc ccttgcggga acatggcatt cccgccgggt tgattactgc tgctacttca
    13261 ccgtttcaga ctaatcctct gtgagagggc gttcatagcc taggaacgga atttctgtcg
    13321 gttgcacatc gcatctgctg tttcgatttc cgaatcaaca tagctatgtt tgacgcaggc
    13381 aaggagcaat cgacgacttt ccttgagcgc gcgcctgcaa cggacaactt cggcttccgc
    13441 ctgtttgagg gcatccacct ttgcttccac tcgggcagcg atcgacagaa tcggatcccc
    13501 gacctcgtag agatagggtg cgaccttctc gattggattg ctcggcactt cagctctcca
    13561 gatcaaagtt cgccgcccag cgtggcaatt cgatcgtttc gatctccccg tttggctgat
    13621 acgccggcca gcggttgttc gcacgacacc attgcaggag ctgcagcgca ccgcgatact
    13681 tggcgcgccc gacgtcgatg acatcctcgc tggcccgata gaccgctgcc gcttgcggag
    13741 cgtccttttc gacggcgatg aagtagaacg ggagcgtcct gcccgtgatg gccttgatgc
    13801 cgtcctgata gaacgccgct tgcaggtcat aaccgagcgt ggcgattgaa cgagcaaatc
    13861 cctctgccga ggcatcggtg caggttttta catccacgat gccggtgatg gtttcatcgg
    13921 ccatgacaag ccagtcgggt cggcacttac atccgagccc ggtctccgga tcactccaga
    13981 agccggacat ttcagccatc ccgttcgcca gcaaacgcga agcgccagtg tgacgagaga
    14041 ggcttgcctg aatggaccgg atggccatca tcagggtggg tgtcaggatt tgcttgccgt
    14101 catacaacct ggcaattgca tcctcccgaa aaacaaatct ggactcgacg tcggctacct
    14161 tgatggcgtc cttgatctct tccttcttca tcttcccgac cttgataccc agttccgcag
    14221 ccgccgcctt gtagtcatcg agcgactgca acgaaccttc gagttgcgac tcgtcgaata
    14281 ccgcgtaatc ggaagcgaat gcggccggtt caagaaccgc ggagtgaacg gccgtaccaa
    14341 atgccattgc cggggtcggc tcgggcggat gggtgacgta ttcctgaaaa tgcgccgggg
    14401 accgcatgat ccgcacaagg cttgaatgcc caacagcgtc cttgttgcgg tggtactcct
    14461 cggcagccat cgacagaagg ccgacttgag tttctataag catttctacc tccagaggca
    14521 agattcccgc tggaggcact cattccctgc cgggaacatg cgtccctggc ggggttggaa
    14581 atgacaggca gttgcctgac tgtcgctaat ggctgtttgt ttcgagcggc cagctaccgc
    14641 ctcttgatgc tcctggttac ccagaagcga caatctggct ttccgcagca caccgcagca
    14701 caccgcgcga tgcgctctgg aaagcccgca ccaaagcgcg ggataggtgg atcacgcatc
    14761 acgcaaacca aaatatccgg tttgcgtgat gcctgacgat caaggggtga gacgcatctt
    14821 gacgacaggt gccagtcgtc atcgcagtcc caacgcgcga acgatggggc ggctgatgat
    14881 gaaaccagaa acggcacaca tcgcgagtat cagaaatatc atggctaggc tccttgtcgg
    14941 gttgatgaat gaaccccaca cgagttgcca ggcttgatgc gcgcccgtaa caaccccgca
    15001 ggcctaagga ctctctttcg aaaatcttta ggcctgcggg attcccacag gcgaggttgg
    15061 tgctggttta gcgcagaccc agcgcccgaa tgatcggacg actgatgacg aaaccaccaa
    15121 tgcagcacgc gataagaacg agttccatga tgtctccttg aaaaagatgc ggagacacca
    15181 agccccaggc ggggagagta tccccgcatg ggtttgaatt gcttcagagc gtcgagttag
    15241 agcaccactt caaaccgggc ttcggtttcg gtgggagcaa caacttgcga ctttttgacg
    15301 gccttctttc ttttggccgg ctttttcggt tgcacgactt ccagcttcgg catcggaata
    15361 ccgcgccgaa taaaggcagg tacgtcgtac ttcgaccagt cctcctcgcc atcgtcatga
    15421 ctgacaagcg aggatgcgga cacggatcgg tgccattccg ggatcttttg cgcgccgggc
    15481 caatgttcag gtgtggccac cggcggtgaa aatgccaggt gtccgaacct gccaacagca
    15541 agcacgaaaa gatggactgt cccgataagc gatgcaacca gaagaacttt ttccatgatg
    15601 cctccaagtc aagaatcctg gagacacctg ctcccattgc gggatgggca tcccgcaggg
    15661 gttacagagt accgccacgc actggcatga acggcacgta aatgctgatt gtttccagcg
    15721 gccagctacc gcttcatcga tggcactagg cacaacgaat gtggagccat tttcactgag
    15781 ccgtttctga tttgcaaccg ctagacggtg cacttttgtg cgcttttcgc attcgattct
    15841 ggcgcctggt tttgtagcat gagcctatct caccaggagt tcatagatgg caaccaggcc
    15901 gcccgcgttc attccatctg cgtcacacga catcagtcgg ccctacttca cgcagaaggt
    15961 aagacttcat tctcttcatg cccagcaggt gtttgatcgc ggtttcgagc tttgcgcaaa
    16021 tgcaattttt tctctgtcag tcgtgttgcg tgtcatcggc actgacgagc aggcgcgcga
    16081 ggtagagacg attgttgatg atcgcctgga caaaatgttc gaagacatgc gttccgaggt
    16141 gactcgactt gaaaagctgg ccgatgcaaa cggtatcgag tttgccggcg tcacctactc
    16201 gaacccgaag gagcttgagg ccaggatcac ctctcctcgc gcagtccgct acatcggact
    16261 catccgcgag tttgatggat tggttggcaa attcgacacc ctttggttat cgggttcgat
    16321 cccggacgga aactattcca gcagcctgta tgaatggaag cgccgcctct tgcgcattgc
    16381 cgggggcttt cgcggtctgg ccagtcgagc gagcatcgcc gcccgaaaga acgctacccg
    16441 tgtggccgag actgactcgg caaccccggc cattgccgta accccaacgg aaacggaatc
    16501 gcctgtatcc gttctcgcgg tataggtgga cctgaatccg caccaggaac gtgcggtttc
    16561 cacggtcggt cactgcactg tccttgcatg tccgggctcc ggtaaaaccc gcgtactttc
    16621 cgagcgagcc attcggttgc tcgcatcgca tgacaaggga cgcttgtgcg ccgtcacctt
    16681 cactcgagac gctgccgagg aactcaaggc acggatcctt ccctcctgtg gtgccgatgt
    16741 tgcgcggcgt ctcgcggtgg ggacttttca ctccatcgca ctttcgcagc tcaagcgatt
    16801 gcctcgtggc cagaagccca ggcgcctgct gtcagacgga gaaaggatgg cggtactgcg
    16861 tcgctgcatc gcccagcatc cgaccgaagc cacgttcgag gatgtcgtca aaaacatcga
    16921 tagtgcaaag gcgaggatca gaacgccagt ctttgcggac ccaggcatcg aggcgatcta
    16981 tatcgcctac caggacaccc tcgcatccga gggcgcgatg gacttcgcgg acatcgtgct
    17041 gtcggctgtc cagaaaatca gctccggcga gttgctgcca ctgcccgtcc gctggctgct
    17101 ggtcgacgaa gcgcaggata tggatgaagt gcaggtggca tgggtaaaag cacatggcct
    17161 cgccggcatc gaagtcaccc tggtagggga tgatgatcaa agtctgtacg ccttccggca
    17221 tgccatgggg tatgcaggtc tgatcgaggt caccgagacc ctggcatcga ccgagacaac
    17281 cctgccgctg aattaccgct gcgcaccaaa catcctggga catgccgcaa agctgatcgc
    17341 tcacaacaag gagcgagcgc cgaagcgcat tgaggctttc agaacatcca ccggcgatat
    17401 ccagatagtc agagcatctg accgttggga tgaggccgat attgtggcgg accggatcag
    17461 gatgttcggc atcgacggcg aatggggaat tctcgggcga accaatagtc agctggatcc
    17521 aatcgagatc tgcctcagcg gttcaggtat tccttacagg cgtaccgggg gcaagaggat
    17581 atgggaccgg aaactcggaa gcgcctttct gggtatccag cgtagcgtgc tcgatggcgg
    17641 ctggacaggc gccgcgaatg ctctggcctt tgctggagtg gcccctgcct acttgaattc
    17701 acgcgaagtt caaggcgaag gtgattgcat gcagtacctc accaggatcc tcgaattgcg
    17761 tggccccgaa ggcgatcacc accagcgagt ggcgggattg ctggccggtt tctcgtcatg
    17821 gaccgagcaa gcaagaaaag gccgggttga actggtggtg catggagtat cagcctggct
    17881 tctacctcac tgcaagaacg atcagcacgt tgccatgctg aatcgactgg agtcatccgt
    17941 cgccaaactg tccggctcac tggggcagcg cttgcggttc ctcacgacag ccgacaacaa
    18001 gcagaagtc
//
LOCUS       DAORPM010000015        17643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_601_length_17643_cov_9.787355, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000015 DAORPM010000000
VERSION     DAORPM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 17643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17643
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_601_length_17643_cov_9.787355"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1269)
                     /locus_tag="PLD53_01520"
     CDS             complement(151..1269)
                     /locus_tag="PLD53_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59613.1"
                     /translation="MSEILRQKRSYRRGLILGLTMAESVILIIFAMLLSLATFLNRKD
                     QAIEQMQGQIADTAADQQRCATALAEAREQLRQLIAKATTDQQHYAKLLAETGVLKAK
                     MVAIQTLTQGTNNFDEMFKELVLMQHQAQRAIELENQVMALTEQQQVLEKVVQRVENT
                     VIGGQSLVEKVDHLIEQAAVWDLLAKFFAPINNPEALHQMEQRLAGLTKLETAVSAVA
                     PGVEAVEAVRTITDQLQASRTQMMNLQGQMANLRRRLEDAGKGTEKPACWAMPDGTPE
                     YIFEVTLRSNGLLVKDNALPNRSDEQKQLPISSINFDREVSLNQFRNAVQPLSKWSQQ
                     HECIFFVKIKDQTGSSQKAIYKRKLRTVGEFFYYYEPP"
     gene            complement(1287..2870)
                     /locus_tag="PLD53_01525"
     CDS             complement(1287..2870)
                     /locus_tag="PLD53_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59614.1"
                     /translation="MNMSYVRTSGQIAYVDRLRDFFDKWLFFIFFTVGAMAIVALKAA
                     EYAQWQVTAAPVGLMLFYAFYAYFSPGFRLREDRIADNLYYLGFLYTLSSLAYSLYLF
                     SQDQANTTTIISNFGIALVTTIVGLALRVLFSQLREDPVEYEREARYELSEAVRHLKT
                     ELDESVLDFNSFRRSLQQSLQEGTDEMLVASKNLLNEHSQQFVHVTQRFVTVFDQSIA
                     TFAHESARANIGIAGVATELASLITRINEIEAPKDLVKHKIEPAVATITQLVEQTFAR
                     TQSETIYFDRLHTLLEKVGGSHQQLEQTLAQLVQANREQATSLQQESQQVRAMVASLC
                     NLVAEASAGVRQHADIQAQQVERFSRNNEALIEKHQQMLGSELQLMRDRLHTLMQEGS
                     ARQATMDKELEQLRLVVTGLGQAHAPLEQLDRNISAQMNALARLTINYEEAAIQAEGY
                     STALNHSLQIVRQSAESLAQSSSDHTEIFNASLQNLEQTLTTAINSLQSTLKEQIQQL
                     VTLPAASGSRSSWFGWLRR"
     gene            complement(2877..3686)
                     /locus_tag="PLD53_01530"
     CDS             complement(2877..3686)
                     /locus_tag="PLD53_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59615.1"
                     /translation="MVRNRRLQRQQTKKRWGAFILLLVFCIVVGGIFGYWWLQQHTIP
                     LNADTLCPIDGPHALTVILVDRTDRFNTVQREAVRVRLDEVQAQTRIYSGIALFSIGP
                     TENELLRPEAYLCNPGPVSEASPLYQNIRRIEQRWHAFIQRLGQAFETAVQSGEQPAS
                     PIMESIQSAALSTFARTAPDTPKRLIIISDLLQHSSTLSLYQQLPLFADFKTTNKFRQ
                     LHAPLIGVEVEVFYLRRPTDRAIQGKALIEFWQQYIRENGGLLVRVVSIEG"
     gene            complement(3676..5001)
                     /locus_tag="PLD53_01535"
     CDS             complement(3676..5001)
                     /locus_tag="PLD53_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59616.1"
                     /translation="MKLEDLGQDRGRQNLPFSDAKLLDDVEQGIITQVENVKQDCMEK
                     LLEHLRTYKDRIAALGFEQKMFEFTNTANNALSDFKAKVHQGHDTLFQLRRDVVRIEI
                     ELIAFREKHQLERMAHYPESQLFHWSIITLILVLESILNGSFLARGHELGLIGGVIYA
                     LAISFVNVGSGLIAGYLLLPQLVHYAWWRKIAGGLAVIILAPFSISFNLAVAHYRDAL
                     GGPLSDDAMHTAWTSFLHNPLGIADIQSWLLFILGLAFFIIALLDGWKMDDPYPRYGR
                     LARKQQTLNDDYAAEKSSLLNELELIRDEAVKKLNQIAEDIQHRKSEYETIIGARHKL
                     TTSFQQHLRHLQQCGNELLAIYREANRRSRTTAIPSHFSETWTLQSPDLPAEVTEGRV
                     SETTLENTVADKFGELKHWSNEIRSAYDTAVMEYRKIEQLTPEVLENGA"
     gene            complement(5215..7059)
                     /locus_tag="PLD53_01540"
     CDS             complement(5215..7059)
                     /locus_tag="PLD53_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRC59617.1"
                     /translation="MSTFDSTKRLLPEILADIVKGTVQLPDFQRGWVWDDDHVRSLLI
                     SVARSFPVGAVMLLETGGEANFQVRPIENVSFNGSPPSADLLILDGQQRLTTLTQVLA
                     LKGAVKTHNDKGKPIERYYYFHIPTALEGPERLEDALLALEPDRKQKANFGRDIVRDL
                     STRALECQQLYFPCGEILNSDDWEESLQEHAAEHFGLYMQFRKVVLNAFRSYQLPIIA
                     LKRETSKEAVCLVFEKVNTGGVALSVFELMTATYAADGYNLRDDWFGSSLRHVESRQK
                     RLAHEPLLNSVETTDFLQAVAMLHTLERRRADLGAGKTGKQVTPVSAKRATILLLPLA
                     AYQKWADRVEAGFLLAAKLLRKEYLTDLSDLPYRTQLAPLAAVLTLLGDHWLEPRIYD
                     KLACWYWCGVLGELYGGAVETRVANDVEELLAWIDDDVELPRTITDAAFQPDRLDRLT
                     SRLSAAYKGLNVLVLREGARDFFWKANIRELDAEDVALDIHHIFPRDWCEKNGIPRKR
                     YNTVVNKTPISYKANRMIGGSAPSQYLQKIQKHSQVLIGDADMNAILGSHFIPADPLR
                     ADDFAAFYQVRKQNLLALIEKAMGKQAVAVAMPEEEEKAETEDADEDS"
     gene            complement(7115..9937)
                     /locus_tag="PLD53_01545"
     CDS             complement(7115..9937)
                     /locus_tag="PLD53_01545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRC59618.1"
                     /translation="MPPKKNNKKNSDSAPTIAVDSLKHTAATRANIPTEELRDFVAED
                     EAAPRTMRYPRNPDLDPQLVWKGKDEQDSADLAVPVVPVYIQEKIHPQAIIEDVQASA
                     KRGQAGTLDLFADFNGIEFDQLIEFYQHNQHWQNRMILGDSLLVMTSLAEKEGLKGQV
                     QMIYLDPPYGIKFGSNWQVSTRKRDVKDGKAEDATRQPEQIKAFRDTWKLGIHSYLAY
                     LRDRLQVARELLTESGSIFVQIGDENVHLVRCLLDEVFGIQNHVATISFQKTGSTDQE
                     LVSQTVDYLLFYSKNIEIIKYHQLYQERGKGTVSLDRYDLMLKSDGILRRLKPEEFIS
                     GELQETGVRVQLTSLSSARPVGSGDLRDFKFNGVTYTPGVGTFKTDLNGLRRLSFAGR
                     VKPTGSTLRYLRWLDDFSVLPISDRWESVQLGTGLIYVVQTSTKVIERCLMMTTDPGD
                     LVLDPTCGSGTTAYVAEQWGRRWITCDTSRVALALARTRLMAARYPYYYLADSENGIK
                     KQAEVTGKLPLAEVKPQGDIRKGFVYKTVPHVTLKAIANNSEIDDIHARWQEKLEPLR
                     AELNALLQQQWQEWEVPREAGEKWPAQAGELLAQWWEHRRQRQREIDDSIARSADTET
                     LYDQPYEDKKRVRVTGPFTVESLSPHRVLSTDEERPASETAAKTEAASASFETMILDN
                     LRKAGVQNTIKNERLKFDALEPFAGAWIHAAGEYAEKDGTVKRVAVSIGPEHGTVGPE
                     QVKEAAKEAVQGIGFDLLIVCGFAFDPHVSEEAKRYGKLTVLPTKMNPDLAMGEDLLK
                     KTGSGNLFMIFGEPDIQPVDPKTGLIQPGADGKIQIELMGLDIYDPTTGQIRSNSTAD
                     IACWFIDTNYNGESFFVRHAYFTGGDKPYEKLQRALKAEVNEAAWAELYSTQSRPFDR
                     PETGKIAVKVINHYGDEVLKVFGVGV"
     gene            complement(9984..10382)
                     /locus_tag="PLD53_01550"
     CDS             complement(9984..10382)
                     /locus_tag="PLD53_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC59619.1"
                     /translation="MPYLLDTNTVIALMKDNPQVIAQVRRVGRAELRLCAPVEAELWF
                     GVSKSGKPEENRQRLLTLLSWLPSLPFSSDAAPHFGDIRAVLARQGLPIGPYDLQIAA
                     IARAQGLVVVTHNTREFARVPDLVLEDWLA"
     gene            complement(10382..10624)
                     /locus_tag="PLD53_01555"
     CDS             complement(10382..10624)
                     /locus_tag="PLD53_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59620.1"
                     /translation="MTLAETIYQHSLHLPEPAARQVLTFIQALEQGYRVAPPVSAASN
                     DTEAFIAAVAGTLGDDFPDDITDDDLGVDAPREALD"
     gene            complement(10658..13717)
                     /locus_tag="PLD53_01560"
     CDS             complement(10658..13717)
                     /locus_tag="PLD53_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRC59621.1"
                     /translation="MADLIENPVINSPFGGPQRHFRFDENGITHEILTGRRLSTYFIP
                     IAKPKLKGAQKQAALDLSVQHRAEENKLINDLRARVSHWRQAGRPYTTALTRRLLEYW
                     TRPDRERRLFFCQIEAVETIIYLTEIAPRHDANQGTFLLEQLKQANAQATPDGQPALL
                     RYAAKMATGSGKTVVMGMLLAWQALNKLANRQDQRFSDAFLIVSPGITIRDRLRVLLP
                     SDPNNYYQFLDLLPPSLREELGKAKVAIINYHAFLRRERNSAASLTKKLLTASGEASP
                     FLETEGQMVARVCADLGGKKNLIVLNDEAHHCYHCKPLDVDEKLTGDDLQEAKQREEE
                     AKIWIGGLGAVQKKLGVRAVYDLSATPFFLRGSGYPEGTLFPWVTSDFSLTDAIESGI
                     VKVPRVPVADNTGQGENPTYRNLWAHVGKDMPKGRGSKEEGPVEPVLPKELQGALHSL
                     YSHYEKAYQQWQAQVQEYPALMPPVFIVVCNNTRSSKRVFDYIAGWDKPLADGAVMPV
                     PGALPIFSNVENGHWSSRPNTLLIDSLQLESGEALSDEFKEVAAREIEEFKDELRARY
                     PGRDVEDVTTEDLLREVMNTVGKRNKLGENIKCVVSVSMLTEGWDANTVTHILGVRAF
                     TTQLLCEQVVGRALRRVSYEPEPHTVIVNGQAVTFEAFPVEYAEVYGVPFEFIPTAGV
                     AKPGPQRNTTQVCTVEARQEAMITFPRLSGYRYELAGETLQAQFTPACRLTLTTRQIP
                     TVVENAPIIGESSIHRLDDLKQRRLNEVAFLLAKLVLEKYFRQDATEYVIGAAVQNEV
                     KAWLLPQVLSIAKRWLAECLELKDETFPQLLLLIELAHDAADRIYQAIVAADSGASTL
                     KPILQPYNTVGDTSGVSFHTSRATWQTNHKCPISHVVLDSGWEGKMAQALEYLDEVYS
                     YVKNHNLDFRIPYTFEGQEHHYLPDFIVRIDDGHGQEDLLNLIIEVSGPALPAKAAKT
                     ATIQNLWVPAVNNAGQFGRWAFVEVKDPDSAMQTVRESLNSRVPSTP"
     gene            13805..13969
                     /locus_tag="PLD53_01565"
     CDS             13805..13969
                     /locus_tag="PLD53_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59622.1"
                     /translation="MGFPGPLRHNSTDDLGLFGRDPALHMHQALLSQALAVAALFVGV
                     AGGHELPYSE"
     gene            complement(14051..14686)
                     /locus_tag="PLD53_01570"
     CDS             complement(14051..14686)
                     /locus_tag="PLD53_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59623.1"
                     /translation="MPPDLALLYEMLVTCEYGQDEFDGEREFLERILPGNQDVMAHSL
                     RRFLMDLLSFDAEPLFFDPPPADEVVQLLEQAAENYGDEQAEADLQRAYHLAPDHLMV
                     LVALFRYYVYQRRFLEAMAISQHARDVIRQSLELPHDWRTLSEAHLVSGGEKSMVRVR
                     CYLLCLKGEAYLYARLGQPEEAIALLEKLIKIDAQDRLGATALLAVVSAGQ"
     gene            14999..15778
                     /locus_tag="PLD53_01575"
     CDS             14999..15778
                     /locus_tag="PLD53_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HRC59624.1"
                     /translation="MIDSDTRSPLTAPPLRNPGQSLDEEIHRLIDEEASGYLLVAFCL
                     LFFTLMEWGRWWFQFPPQPALWTLVTVVVVAYVTRKLFVLRRKVRQLRLARDGERAVG
                     QFLETLRARGYRVLHDLIGEGFNVDHALIGPAGIFTVETKTYHKPAKGKPEIVYDGER
                     ITVAGYVPERNVVVQGKAQAHWLQNVLLESTGQRFKVQSVVLYPGWFVKTTIKLNGDL
                     PWILNPKALPAFLEHQPAQLSDADVQLATYHLSRFMRAVAK"
     gene            15801..16085
                     /locus_tag="PLD53_01580"
     CDS             15801..16085
                     /locus_tag="PLD53_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59625.1"
                     /translation="MTDSAYPAPHRQTLDDLDPESATTDRLIAMVRSHRQGEEYPTAE
                     TLLGNLPIVLRALCDHLLSGEATALDVAYRIASLIDAIEGCPAPPHQHLH"
     gene            16155..16742
                     /locus_tag="PLD53_01585"
     CDS             16155..16742
                     /locus_tag="PLD53_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRC59626.1"
                     /translation="MPKVADDAAYLARLQDYFARWRSLPAYEPLQAVLGLASRSAVAK
                     VLHRLQGAGFVERTPDGRWTPTGRFFERPHAECPVPAGVPLATADSGDVRSIDAWLVR
                     HPSRTVLIPITGDSMIEAGIHSGDVAVVERDAPARPGDPVIAVIDNEFTLKTLAVEDG
                     EAVLQPANPAYSTLRPGDRLQVFGVVVGLIRNYRR"
     gene            16748..17215
                     /locus_tag="PLD53_01590"
     CDS             16748..17215
                     /locus_tag="PLD53_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRC59627.1"
                     /translation="MLAVLPEPATARAVPLWLDPDLNREAESRPGGSWRADFPSPAND
                     DVEQSLNLNDHLIRNPEATFLMRVTGEALAEAGLHDGDLLVMDRATPPLAGSVVLVVV
                     EGVAVLTRLSRDAQGRLRVPAPGTDLSQPLDEATIAGVARWVIHRLWPGRLSS"
     gene            complement(17302..>17643)
                     /locus_tag="PLD53_01595"
     CDS             complement(17302..>17643)
                     /locus_tag="PLD53_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC59628.1"
                     /translation="IHDQASRPHPKLMTTDVAAAKQFYGALFGWQTKDDPGGEGYAMV
                     NVGGEAVDGMRSAWGVYVTVDDVDATAQQVEALGGKVLCPLKDIPNIGRCCFFRDPQG
                     ATLYAITYRKP"
BASE COUNT         3676 a         5062 c         4803 g         4102 t
ORIGIN      
        1 ttcttcaata agtgaataat taatagtttg ctttgtaccg ccacactcag gacatacata
       61 cggcctctga gatgccagaa gttcatcaag cttttgttta caatcagaac aacaaacgcc
      121 atcttcaacc atgttgaatc ctaaaaattc ttatggcggt tcatagtaat aaaagaactc
      181 tccaacggtt cgtaattttc gcttgtagat cgctttttga ctcgatccag tttgatcttt
      241 aatcttgaca aagaaaatgc actcatgctg ctgactccat tttgatagcg gttgcacagc
      301 attacggaat tgattgagtg aaacctcacg atcgaaattg atactgctga taggtaactg
      361 cttttgttca tctgatcgat tgggaagtgc gttatccttt accaataacc cgtttgaccg
      421 cagagttacc tcgaagatgt attctggtgt accatctggc attgcccaac aagcaggttt
      481 ttcagtacct ttgccagcgt cttccagtcg ccggcgtaga ttggccatct gtccctgcaa
      541 gttcatcatc tgtgtgcggg atgcctgaag ttgatcagta atcgttcgaa ctgcttccac
      601 tgcttctaca cccggtgcga cggctgaaac agcagtttcc aattttgtta atcccgccag
      661 acgctgctcc atctgatgca acgcttccgg attattaatt ggcgcaaaga actttgctag
      721 caagtcccat acggcagctt gctcaatcaa atgatcgacc ttttctacca gagattggcc
      781 accgattact gtattttcta cacgttgcac aactttttct agcacctgct gttgttcagt
      841 gagcgccata acttgatttt caagctcgat ggcgcgttga gcctgatgct gcattagcac
      901 cagctctttg aacatttcat caaagttatt agtgccctga gtcagcgttt ggattgccac
      961 catctttgcc tttagcactc ctgtctccgc taataatttg gcatagtgct gctgatcagt
     1021 agtggcttta gcgatcagtt gccgtaactg ctctctcgct tccgctaagg cggtggcaca
     1081 acgctgttga tctgccgcgg tatccgcaat ctgcccctgc atctgctcga tagcctgatc
     1141 tttacggttt aggaacgtag ccagcgatag gagcatggca aaaataatta gaatcacgct
     1201 ttccgccata gtcaatccaa gaatcagacc acgccggtag gagcgctttt gtcgcaggat
     1261 ttcgctcata gaatcacagg ggagcgctaa cggcgcagcc aaccaaacca actgctgcga
     1321 gaaccactag cggctggtag cgtgacgagt tgttgaatct gctcttttaa cgtactttgt
     1381 aggctgttta tcgctgtggt gagagtctgt tccaagtttt ggaggctagc gttgaatatc
     1441 tcagtatggt cactgctgct ctgtgccagc gactctgccg attggcgaac aatttgcaga
     1501 gaatgattca gagccgtgct ataaccttca gcttgaatag cagcttcctc atagttaatt
     1561 gtcagccgcg ccaaggcatt catttgggct gagatgtttc ggtcaagttg ttccaatggt
     1621 gcgtgtgcct gcccaagacc agtcacaaca agtcgtagtt gttccagttc cttatccata
     1681 gttgcctgac gagcagaacc ttcttgcatc aaagtgtgca gtcgatcacg catcaattgc
     1741 aattctgatc ctaacatttg ctgatgtttc tctatcagag cttcattatt gcggctgaac
     1801 cgttcaactt gttgcgcttg gatatctgca tgctgccgaa cgccggcgct ggcttcggct
     1861 accaaattgc agagggatgc gaccattgcc cgcacttgct gactttcttg ctgcaggctc
     1921 gtagcctgct cacggttagc ctggaccagt tgagctaggg tttgttcaag ttgttggtga
     1981 cttccgccca ccttttcaag caacgtatga aggcgatcaa aatagatcgt ttcgctttgc
     2041 gtgcgggcaa acgtctgttc aactagttgg gtaatggttg ccaccgctgg ctcgatttta
     2101 tgctttacta aatcctttgg cgcttcaatc tcattgatac gtgtgattaa actagctaat
     2161 tcggtagcca cgccagcgat gcctatattg gcgcgagccg attcatgggc gaaagtcgct
     2221 attgactgat cgaaaacagt gacaaaacgt tgggtaacat ggacgaattg ctgactgtgc
     2281 tcgttgagca gattcttgga agcgaccaac atttcatctg tgccttcctg cagggattgc
     2341 tgcagggatc gccgaaagga attgaaatct agtacgctct catcgagttc ggtctttaga
     2401 tggcgaaccg cttcgctcaa ttcatagcgt gcctcgcgct catactctac gggatcttcg
     2461 cgtagttggc taaacagcac ccgcaacgcc aaaccaacga tggtggtcac cagtgcgatg
     2521 ccaaaattag agatgattgt tgtcgtatta gcttgatctt ggctaaaaag atacaacgaa
     2581 tacgccaagc tagagagggt gtacagaaaa cccaagtaat acaagttgtc ggcaatgcga
     2641 tcctcacgta gccgaaagcc tggcgagaaa taagcgtaaa acgcatagaa caacattaaa
     2701 cctacagggg cagcagtaac ttgccactgg gcgtattctg cggcttttag ggccacaatc
     2761 gccattgccc caaccgtgaa gaaaatgaag aacaaccatt tatcaaaaaa atcacgtagt
     2821 cggtctacat aggcaatttg gccggaagtg cgtacatagc tcatattcat attgtcttat
     2881 ccttcaatgg acactacccg taccaacaga ccaccgtttt cccggatgta ttgttgccaa
     2941 aattcgatca gcgccttgcc ttgaattgca cggtcggtcg gtcggcgaag gtaaaaaacc
     3001 tccacttcga ccccgattag aggcgcatgc aactggcgga atttattagt ggttttgaaa
     3061 tcggcaaaga gaggcaattg ctgatacagt gagagcgtcg agctgtgttg gagcaggtct
     3121 gatatgataa tcagccgctt aggggtgtcg ggtgctgtcc tagcgaaggt agaaagtgct
     3181 gctgactgga tgctttccat aataggtgaa gctggttgct caccagactg cacagccgtc
     3241 tcgaaagctt gccctagacg ctgtatgaat gcatgccatc gttgttctat ccgacgaata
     3301 ttttgataaa gaggtgaggc ttcgctgacc ggacctggat tgcacaggta cgcttcagga
     3361 cgtagtaact cattttcagt cggtccaatc gagaacaggg caatcccgct atatatgcgg
     3421 gtttgcgctt gcacctcatc caaccgcaca cgcacggctt ctcgttgcac ggtattgaat
     3481 cggtcggttc gatccaccaa gatgacagtc aaagcgtgcg gcccatcaat cgggcaaagc
     3541 gtatcggcgt tcaacgggat tgtgtgctgt tgcaaccacc aatagccaaa aataccgccg
     3601 acaacaatgc agaataccaa cagaaggata aatgcacccc atcgtttttt tgtttgttga
     3661 cgctgtaaac gccgattacg caccattttc cagcacctca ggagttagtt gctcgatctt
     3721 gcgatattcc attacagcag tgtcataagc ggaacggatc tcattcgacc agtgttttag
     3781 ttctccaaat ttatcagcca cagtgttttc aagtgtagtt tcggaaaccc gaccttccgt
     3841 aacctctgcg ggtaaatctg gcgactgaag cgtccaagtc tcagaaaaat gagatggtat
     3901 ggctgtagtg cgagaacgtc tgttagcttc tcggtagata gcaagcaatt cgttgccaca
     3961 ttgctgcaaa tggcgcagat gctgctggaa tgaagtcgtt agtttatgac gtgcgccaat
     4021 gatggtctca tattcacttt ttcgatgttg aatgtcttcg gcaatctgat tcagtttctt
     4081 tactgcttca tctcgaatta attccaattc attcaataaa ctgctttttt ccgcagcata
     4141 gtcgtcattg agagtttgct gcttacgagc tagccggccg taacgcggat aggggtcatc
     4201 cattttccag ccatcgagta gggcgataat gaagaatgct aaccccagga taaatagaag
     4261 ccatgactga atatcggcta tacccaaggg gttgtggaga aaactggtcc aagcagtgtg
     4321 catagcatca tcggatagtg gaccgcctag cgcatcccga tagtgagcaa cagccagatt
     4381 gaagctaata gaaaaggggg ccaatataat cactgctaga ccaccggcga tcttgcgcca
     4441 ccaagcataa tgtacaagtt gcggcaataa taaatatcct gctatcaggc cgcttccaac
     4501 attgacaaaa gagattgcta acgcataaat aacgccacca attaagccaa gttcgtggcc
     4561 acgagccaga aagctgccat tgagtatact ttctaatact aaaatcagtg tgatgatact
     4621 ccaatgaaat aattgtgatt caggataatg agccattcgc tccaattggt gtttctcacg
     4681 aaaggctatg agttcgatct cgatgcggac tacgtcgcgg cgtagctgaa ataaagtatc
     4741 gtggccttga tgaacttttg ccttgaaatc gctgagtgcg ttgttagccg tattggtaaa
     4801 ctcaaacatc ttttgctcga agcccagtgc ggcaatacga tccttgtaag ttctcagatg
     4861 ctctagcaac ttttccatgc agtcctgttt cacattctcg acttgagtga taataccttg
     4921 ttctacgtca tcaagaagct tagcgtcaga gaatgggaga ttttggcgcc cgcgatcctg
     4981 tcctaaatct tccagcttca tgcgcttctt agtaactacc gtgtcgagtt gcggaaaaaa
     5041 atctggcgag actcgataat tgtgtcgtcg gttaaaaatc ataagttggc tatgatatcc
     5101 aagcgattga gtgttaattt ctccaaggtt gccaaactca aaaattgagg cacaaaaatg
     5161 gatgtgacca caattatctg gttagtcttg aagtgaagta gtgtatcgct accctcagga
     5221 atcctcatcg gcatcctccg tctccgcttt ttcttcctcc tccggcattg ctaccgccac
     5281 ggcctgcttg cccatcgcct tctcgatcag cgccagcaga ttctgtttgc ggacttggta
     5341 gaacgcggcg aaatcatcgg cgcgtaacgg gtcagcgggg atgaaatggc tgcctaaaat
     5401 ggcgttcatg tcggcatcgc cgatcaacac ttgggaatgc ttctgaattt tttgcaaata
     5461 ctgcgagggc gccgagccgc cgatcattcg gttggccttg taagaaatcg gcgtcttgtt
     5521 gaccacggtg ttgtaacgct tgcggggaat accgtttttc tcgcaccagt cgcggggaaa
     5581 gatatggtgg atgtccagcg ccacgtcttc ggcatccagc tcccgaatgt tggctttcca
     5641 gaagaaatcc ctggcccctt cgcgcaacac caacacattc agccccttgt aggccgcgct
     5701 aagccgggag gtcagtcggt ccaaccggtc gggctggaaa gcggcgtcgg tgatggtgcg
     5761 cggcaattcc acatcgtcgt cgatccaggc cagcagttcc tcgacatcat tggcgacgcg
     5821 ggtttccacc gcgccgccgt acagttcgcc cagcacccca caccagtacc agcacgccag
     5881 cttgtcgtag atgcgcggct ccaaccagtg atcacctaac aaggtcaaga cggcggccag
     5941 cggcgccagt tgggtgcggt aaggtaagtc gctgagatcg gtcaggtatt ccttacgcag
     6001 caacttggcc gccaagagaa aaccagcctc aacccgatcc gcccatttct ggtaagccgc
     6061 cagcggcaac agaagaatcg tggcgcgttt ggcgctcact ggggtaacct gtttgccggt
     6121 tttgcccgcc ccgaggtctg cccggcgacg ctccagcgta tgcagcatgg caacggcttg
     6181 caggaagtcg gtggtttcga cgctgttcag caagggttcg tgggccaggc gcttctggcg
     6241 tgattccaca tgacgcaggc tgctgccaaa ccagtcgtcg cgtaggttat agccatcggc
     6301 ggcataggtg gcggtcatca attcgaaaac ggacagcgca acgccgccgg tgttgacctt
     6361 ttcaaatacc aggcaaaccg cttctttcga ggtttcccgt ttgagggcaa tgatgggaag
     6421 ctggtaactg cggaaggcgt taagaaccac cttacgaaac tgcatgtaca ggccaaaatg
     6481 ctcagcagca tgttcctgca agctttcttc ccagtcatcc gagttcaaga tttcgccgca
     6541 tggaaaataa agttgctggc actccaaagc gcgcgtggag agatcgcgca cgatatcgcg
     6601 accaaagttg gctttctgtt tacggtccgg ctccagcgcc agcaacgcat cttccaaccg
     6661 ttccggacct tccagagcgg tcgggatgtg gaagtagtaa tagcgctcga taggtttgcc
     6721 cttgtcatta tgggttttta ccgccccttt gagcgccaac acctgggtca gggtcgtcaa
     6781 ccgttgttgg ccgtccagaa tcagcaaatc cgcgctgggt ggagaaccgt taaaggagac
     6841 attctctatc gggcgcacct gaaaattggc ttcaccgccc gtttccagca gcatgaccgc
     6901 gcccaccggg aaggatcgcg ccacgctaat cagcaggctg cgtacatggt catcgtccca
     6961 gacccatccg cgttggaagt cagggagttg aaccgtgcct ttgacgatgt cggcaaggat
     7021 ttcgggaagc aggcgcttag tgctatcaaa ggtactcatc agaatctctt gtgattttta
     7081 tcttgcccat cttgtggtta atgtctttga ggcttcatac ccccacccca aacaccttca
     7141 acacctcatc cccgtagtga ttgatcacct ttaccgcgat cttgccggtc tcgggccggt
     7201 caaacgggcg gctttgggtc gagtacagtt ccgcccaggc ggcttcattc acctcagcct
     7261 tgagcgcccg ttgcaacttt tcatagggct tatcgcctcc cgtgaaatag gcatgacgga
     7321 caaagaagct ctcaccgttg taattcgtgt cgatgaacca gcaggcgata tccgccgttg
     7381 agttggagcg aatctgcccg gtcgtcgggt cgtagatgtc cagccccatg agttcgatct
     7441 gaatctttcc atccgcgccc ggttgaatca ggccggtttt ggggtccact ggctggatgt
     7501 ccggctcgcc gaagatcatg aacaggttgc cgctgccggt cttcttgagc aggtcctcgc
     7561 ccatcgccaa gtcagggttc atcttggtag ggagtacggt cagcttgccg tagcgtttcg
     7621 cttcctcgga gacatgcgga tcgaaggcaa aaccgcaaac gatgagcaag tcaaagccaa
     7681 taccttgcac cgcctcttta gcggcttcct tcacctgctc cgggccgacc gtgccgtgtt
     7741 ccgggccgat ggacaccgcc acccgcttca ccgtgccgtc cttctccgcg tactcgcccg
     7801 ccgcgtgaat ccaggccccg gcgaacggtt ccagggcatc gaacttcagc cgctcgttct
     7861 tgatggtgtt ctgcacccca gccttgcgca ggttgtcgag aatcatggtc tcgaacgagg
     7921 cgctggcggc ttccgtcttg gcggcagtct cggacgcggg ccgctcttca tcagtcgata
     7981 gaacccgatg cggcgacagg ctttccacgg taaacgggcc ggtgacccgc acccgcttct
     8041 tgtcttcgta gggctgatcg tagagcgtct cggtgtcggc gctgcgggcg atggaatcgt
     8101 caatctcccg ctggcgctgg cggcgatgct cccaccattg cgccagcaac tccccggcct
     8161 gggcgggcca cttctcccca gcctcgcgcg gaacctccca ttcctgccat tgctgctgga
     8221 gcagggcgtt caactcggct cggagcggct ccagcttctc ctgccagcgg gcgtgaatgt
     8281 cgtcgatttc cgagttgttg gcgatggcct tgagggtgac gtgcgggacg gtcttgtaaa
     8341 caaagccctt gcgaatatcg ccctggggct tcacttcggc cagcggcaac ttgccggtaa
     8401 cttccgcttg cttcttgatg ccgttctcgg aatccgccag gtagtagtag ggatagcggg
     8461 cggccatcag gcgggtgcgg gccagggcga gggccacgcg ggaggtgtcg caggtgatcc
     8521 agcgccggcc ccattgctcg gcgacgtagg cggtggtgcc gctgccgcaa gtggggtcga
     8581 gaactaaatc gccggggtcg gtggtcatca tgaggcagcg ttcgatgacc ttcgtacttg
     8641 tctggacgac gtagatcaac ccggtgccaa gctgaacgga ttcccatcga tcactgatag
     8701 gtagaacaga gaaatcatca agccatcgta aatatcgaag ggtactgcca gtgggtttaa
     8761 cacgtcctgc aaaggatagc cgtctaagtc cattcaaatc tgtcttaaat gtgcctacgc
     8821 caggggtgta agtaacacca ttaaatttga aatctcggag atcacctgat ccaaccggtc
     8881 tcgcgctgct taacgatgtt agctgaacac gaaccccagt ttcttgcagt tctcccgaaa
     8941 taaattcttc aggttttagt cgacgcaaaa taccatcaga tttcaacatt agatcatagc
     9001 gatctagtga aacagtgcct ttacctcgct cttggtatag ctgatgatac ttaataattt
     9061 caatattctt tgaatagaag agcaggtaat caactgtttg tgaaacaagc tcttgatctg
     9121 ttgatcctgt tttctgaaag ctgattgtcg caacgtgatt ttgaatccca aacacctcat
     9181 ccaacaaaca ccgcaccaaa tgcacattct catcgccgat ctgcacgaag attgacccgc
     9241 tctccgtcaa taactccctc gccacctgca accgatcccg cagataggcc agataggaat
     9301 ggatgcccag cttccaggta tcccgaaacg ccttgatctg ctccggctgg cgggtggcgt
     9361 cttcggcctt gccgtccttc acgtcccgct tgcgggtgga tacctgccag ttggagccga
     9421 acttgatgcc atagggcgga tcgaggtaga tcatctgcac ctggcccttg agtccctcct
     9481 tctcggccag gctggtcatc accaggaggg aatcgcccag gatcatgcgg ttctgccagt
     9541 gctgattgtg ctggtagaac tcgatcaact ggtcaaactc gatgccgttg aagtcggcga
     9601 acaggtccag cgtgcccgcc tggccccgct tggcgctggc ctgcacgtcc tcgatgatcg
     9661 cctgcgggtg aatcttctcc tggatgtaga ccggcaccac cggcaccgcc aggtcggcgc
     9721 tgtcctgctc gtccttgccc ttccacacca gttgcggatc gaggtcggga ttgcggggat
     9781 agcgcatcgt cctgggcgca gcttcgtcct cggccacaaa gtcccgcaat tcctcagtgg
     9841 ggatattggc gcgggtggca gccgtgtgct tgaggctgtc cacggcgatg gtcggggcgg
     9901 aatccgaatt tttcttattg ttctttttgg ggggcatgat caagcggcct ttcaaaacct
     9961 ctcagtgggc aacagggtgg atttcaagcc aaccagtctt cgagcaccaa gtcgggcaca
    10021 cgggcgaatt cacgggtgtt gtgcgtgacg acgaccaaac cttgagcgcg ggctatcgcg
    10081 gcgatttgca gatcgtaagg gccgatgggc agtccttgac gcgccaagac cgcccgaatg
    10141 tcaccgaagt ggggggcggc atcgctggaa aagggcaggc tcggcaacca cgacaagagc
    10201 gttaacaagc gttgccggtt ttcttcgggc ttaccgctct tggagacacc aaaccacaat
    10261 tccgcctcga ccggggcgca aagccgcaat tcggctcgac ccacccgtcg cacttgggcg
    10321 atcacttggg ggttgtcctt catcaacgca atgacggtgt tggtatcgag gagataaggc
    10381 atcagtccaa cgcttcccgt ggcgcatcga cgcccaagtc atcatcggta atgtcatcgg
    10441 ggaaatcatc gcctagcgtt ccggcgacgg cggcaataaa tgcctccgtg tcattggagg
    10501 ctgcggacac ggggggagcc acgcggtagc cttgctccaa agcttgaatg aacgtcagca
    10561 cttgccgggc ggcaggttca ggcaaatgga ggctgtgttg ataaatcgtt tcagcgagtg
    10621 tcatcagagg tctcctcatt ctaggagtag cgcggcatta gggagtcgac ggaacgcgag
    10681 agtttagaga ctcccgcacg gtttgcatcg cgctgtccgg gtctttcact tctacgaacg
    10741 cccagcgccc gaactggccg gcgttattca ccgctggaac ccacagattt tgaatcgtgg
    10801 ccgtcttcgc cgccttggcg ggcagcgcgg ggccggacac ctcgatgatc aagttcaaca
    10861 agtcttcctg gccgtggccg tcatcgattc gcacgatgaa atccgggagg tagtggtgtt
    10921 cctggccctc gaaggtatac ggaatccgga agtccaggtt gtggttcttg acgtagctgt
    10981 aaacctcatc caggtactcc agcgcctggg ccatcttccc ctcccaaccg ctgtccagca
    11041 ccacatggga aatcgggcat ttgtggttgg tctgccaggt ggcccggctg gtgtgaaagc
    11101 tcactccgct ggtgtcgccc acggtgttgt acggttgcag gatcggctta agggtgctgg
    11161 ccccgctgtc cgccgccacg atggcctgat agatgcggtc ggcggcatcg tgcgccaact
    11221 cgatcagcaa cagcagttgc gggaaggttt catccttcag ctccagacat tccgccagcc
    11281 agcgtttggc gatgctcaac acttgcggca acagccacgc cttgacctcg ttttgcacgg
    11341 cggcgccgat gacgtattcg gtggcgtcct gccgaaaata cttttccagc accagtttcg
    11401 ccagcaggaa cgccacctcg ttcaagcgcc gctgtttgag atcgtccagc cggtgaatgc
    11461 tggactcgcc gataatcggc gcattctcca ccacggtggg gatttgccgc gtggtcagcg
    11521 ttagccgaca cgcgggggtg aactgagcct gtagcgtctc cccggccagt tcgtagcggt
    11581 agccgctcaa acgcgggaag gtgatcatgg cctcctgccg cgcctctacg gtgcagactt
    11641 gcgtggtgtt tcgttgcggg ccgggcttgg cgacgcccgc cgtggggatg aattcaaacg
    11701 gcacgccgta gacttcggcg tattcgaccg ggaacgcctc aaaggtcacg gcctggccgt
    11761 tgacgataac ggtgtgcggc tccggttcgt agctcactcg gcgcaacgcc cgcccgacca
    11821 cctgctcaca caataactgg gtggtaaacg cccgcacccc caagatatgg gtgacggtat
    11881 tggcgtccca gccctcggtg agcatcgaca ccgacactac gcacttgatg ttttcaccca
    11941 atttattgcg cttgccgacg gtgttcatca cctcgcgcaa caggtcttcc gtggtcacgt
    12001 cttctacatc ccgccccgga taacgcgctc gcagctcgtc cttgaactcc tcaatctcgc
    12061 gggcggcgac ttccttaaac tcgtcgctca aagcctcgcc ggattccagt tgcaggctgt
    12121 caatcaacag cgtgttgggg cgactcgacc agtggccgtt ctcgacgttg ctgaaaatcg
    12181 gcaacgcgcc cggcaccggc atcacagcgc catccgccag cggcttatcc caaccggcga
    12241 tgtagtcaaa cacccgcttg gaggaacgag tgttgttgca gaccacgatg aacaccggcg
    12301 gcatcagcgc cgggtactct tggacttgcg cttgccactg ctgataggct ttttcgtagt
    12361 ggctgtagag actgtgcagc gcgccctgca actctttggg caagaccggt tccaccggcc
    12421 cctcttcctt gctgcctcgg cccttgggca tgtccttgcc gacgtgcgcc cacaggttgc
    12481 gataggtcgg attttcgcct tggccggtgt tgtcggccac cggcacccga ggcaccttca
    12541 caatgccgct ttcaatcgca tccgtcaggg agaaatccga ggtcacccag gggaacagcg
    12601 tcccttccgg gtagccggac ccccgcagga aaaacggggt cgccgatagg tcgtacaccg
    12661 cccgaacgcc cagcttcttc tgcaccgcgc ccaggccgcc gatccagatt ttcgcttcct
    12721 cctcgcgttg tttcgcttcc tgcaagtcgt ctccagtgag cttctcatcc acgtccagcg
    12781 gcttgcagtg gtagcagtga tgggcttcgt cgttcagcac gatcaggttt ttcttgccgc
    12841 ccaggtcggc gcacacccgc gccaccatct gcccctcggt ttccagaaag gggctggcct
    12901 cgccgctggc agtgagcagt ttcttggtca atgacgccgc gctgttccgt tcccgccgca
    12961 ggaaggcgtg gtagttgatg atggcgactt tggccttgcc caactcttcc cgtaggctgg
    13021 gcggtaacaa gtccagaaac tggtagtagt tgttcggatc gctgggcaac agcacccgca
    13081 agcggtcgcg gatggtgatg cccggcgaca cgatcaggaa ggcgtccgag aatcgttgat
    13141 cttgacgatt cgccagcttg ttgagcgcct gccaggccag cagcatcccc attacgacgg
    13201 tcttgccgct gccggttgcc atcttggcgg catagcgcag tagcgccggt tgcccgtctg
    13261 gcgtcgcctg agcattggcc tgtttcagtt gctccaggag aaaagtgccc tgattggcgt
    13321 cgtggcgggg cgcgatctcg gtcaggtaga tgatcgtttc tacggcctcg atctggcaga
    13381 aaaacaaccg ccgctcccgg tcgggacggg tccagtactc cagcaagcgc cgcgtcaggg
    13441 ccgtcgtgta gggtcgcccg gcctgccgcc agtgagaaac ccgcgcccgg agatcattga
    13501 tcagtttgtt ttcttcggcc cggtgttgaa cgctcaaatc cagcgccgcc tgcttttgcg
    13561 cgcctttgag cttgggcttg gcgatgggaa tgaaataggt actcagccgc cgtccagtca
    13621 gaatctcatg ggtaatcccg ttctcatcaa accggaaatg gcgctgcggc ccgccaaacg
    13681 gcgagttaat gacggggttt tcgatgaggt cggccatgcc ttggctcttg gtagtccggc
    13741 gcgatcaggc cggattctga ctgaaacagg ctccagtttc tctcttccgg ccctgcgtcc
    13801 cctcgtgggc ttccccggcc cgctccgcca taattcgacg gatgacctcg gcctgttcgg
    13861 gcgtgaccca gccctgcaca tgcaccaggc cctgctctcg caggcgctgg cggtagcggc
    13921 gctgtttgtc ggcgtggcgg gaggccatga gctaccctat agtgagtagg gtcacatgtt
    13981 accacaaacc agtgactgtc caatgtcggc agtttcgccc ctgcgctgct aaaagcgagg
    14041 gtgcctgggt ttactgtcct gcgctgacga cggccaacag ggcagtagcc cccagacgat
    14101 cctgggcatc gatcttaata agcttttcca gtaaagcgat ggcctcctcc ggctgaccga
    14161 gccgggcata caggtaggct tcgcctttca ggcacagcaa atagcagcgg accctgacca
    14221 tgctcttttc accccctgaa acaaggtggg cttcgctgag agtgcgccaa tcgtgcggca
    14281 actccagtga ttgccgaatg acatcccgcg cgtgttggct gatggccatg gcctccagaa
    14341 agcgccgttg atagacgtaa tagcgaaaga gggcgaccag caccatgaga tgatccggag
    14401 ctaaatgata ggcgcgctgt aaatcagctt ccgcttgttc gtcaccgtaa ttttctgcgg
    14461 cctgctccaa taattgaacg acttcgtcgg ccggcggagg gtcgaaaaac agcggttcgg
    14521 catcgaacga caataaatcc atcaggaacc tcctcagtga atgagccatc acatcctggt
    14581 tgccgggcag gatgcgctcc agaaactccc tctcgccgtc gaactcgtcc tggccgtact
    14641 cgcacgtcac cagcatctcg tacagcaggg ccagatcggg cggcaccggg tgtctccggg
    14701 gtcaagggtc gcgcgagggt ttgctacagt gtagcccctt ctacttcgac cggaccgccg
    14761 ccatggctcg acgtccccgc tcccgcgcct tcccgaaaac cctgaccggc atcctcgcgt
    14821 ctatcgccct ggccgtccgc gccgcccctg ccaggggcag gccccatacg aactttcaaa
    14881 agtctagccg caatccctat ccagtacgcc ctcaccgcat ggttccggcg aaatcgggag
    14941 ccagaaggat gctaccctgt tgatcaggac cgccttccag acactttcca cgcctttcat
    15001 gatcgattcc gacacccgct cacccctgac cgcaccgccg ctgcgcaatc ccggccaatc
    15061 cctcgatgaa gaaattcacc gcctcatcga cgaggaggcg tccggctact tgctcgtcgc
    15121 cttctgcttg ctgttcttca ccctgatgga atgggggcgc tggtggtttc agtttccgcc
    15181 gcaacccgcc ctgtggaccc tggtgacggt cgtggtcgtc gcctatgtca cccgcaagct
    15241 gttcgtactc cggcgcaagg ttcggcaatt acgcctggcg cgcgacggcg aacgcgcggt
    15301 cggtcaattt ctggaaacct tgcgggcgcg cggctaccga gtgctacacg atctgatcgg
    15361 cgagggcttc aatgtcgatc acgcattgat cggtcccgcc gggatcttca cggtcgaaac
    15421 caagacctac cacaagcccg ccaagggtaa gccggagatc gtctatgacg gcgagcgcat
    15481 caccgtggcc ggctacgttc ccgagcgcaa tgtggtggtg caaggcaagg ctcaagcgca
    15541 ttggttgcaa aacgtgctct tggaatccac cggacagcgg ttcaaggttc agtcggttgt
    15601 gctgtacccg ggttggtttg tgaagacgac catcaagctg aacggggatc ttccttggat
    15661 actcaacccc aaggcattgc cggccttcct ggaacaccaa ccggcccagc tatcggatgc
    15721 ggacgtgcaa ctggcgacct atcacctcag ccggtttatg cgcgccgtag cgaaataacc
    15781 tcttctatcg gcatccctcc atgactgatt ccgcctaccc tgccccgcat cgccagaccc
    15841 ttgatgatct cgaccccgag agcgccacca ctgaccggct gattgcgatg gttcgcagtc
    15901 accgccaggg cgaggagtac cctaccgctg aaactctgct cggcaatttg cccatcgtgc
    15961 taagggcgct gtgcgatcac ctgctgtcgg gcgaggcgac cgccctggat gtcgcttacc
    16021 ggattgcatc cctcatcgat gccattgagg gctgcccggc cccaccccac cagcacctcc
    16081 actgaatccc cgcttgacgg gctaatgttt gcgttctatg atgagagtac aaatgtactc
    16141 tatcacgagc cgccatgccc aaagtcgccg atgacgccgc ctatctggcc cgcctccagg
    16201 attatttcgc ccgctggcgc agcttgcccg cgtatgagcc gttgcaggcg gtgttggggc
    16261 tggcctcgcg ttcggcggtc gccaaggtgc tgcaccggtt acagggagcg gggtttgtcg
    16321 aacggacgcc ggacgggcgc tggacgccga ccgggcgctt ctttgaacgc cctcatgcgg
    16381 aatgtccggt tccagcaggc gtaccgctgg cgactgcgga tagtggagat gtccggtcga
    16441 ttgacgcttg gctggtacgc catccctcgc gcaccgtact cattcccatc accggcgact
    16501 cgatgatcga ggccggcatt cattccggtg atgtagccgt cgtggaacgc gacgcaccgg
    16561 cccggcccgg cgatcccgtc atcgcggtca tcgacaacga attcactctc aagacgttgg
    16621 cggtggagga cggcgaagcc gtgttgcaac cggccaatcc tgcctattcg accctgcgcc
    16681 ccggcgaccg tttgcaggtc tttggggtgg tggtgggctt aattcgcaac tatcggaggt
    16741 aatgctcatg ctcgccgtac tccccgaacc cgcaaccgcg cgcgctgttc ccctctggct
    16801 ggacccggac ttgaatcggg aggccgaatc gaggcccggc gggtcctggc gggctgactt
    16861 cccctctcca gcgaatgacg atgttgaaca gtctctcaac ctcaacgatc atctgatccg
    16921 taacccggaa gccacttttt taatgcgcgt gaccggggag gctctggccg aggccgggct
    16981 tcacgatggg gatttactgg tgatggatcg ggcgacgccg ccgctggccg gcagtgtggt
    17041 gctggtagtc gtggaggggg tcgctgtcct cacccgcctg agccgcgatg cccaaggccg
    17101 gttgagggtg ccagcgccag gaactgacct cagccagccg ctggatgaag caacgattgc
    17161 cggggtagcg cgctgggtga ttcaccggct ctggccggga cggctgtctt cctgaccttt
    17221 cacagaggct ttcctaatct ttcctaaccg acgaggcgat gacaatgagc gaaaacttca
    17281 tgaaacacgg tatccgggag ttcagggctt ccgataggtg atggcgtaca gcgtcgcccc
    17341 ctgcgggtct ctgaagaagc agcaccggcc aatgttagga atgtctttca gcgggcacaa
    17401 cactttcccg cctaacgcct ctacctgctg ggccgtcgcg tccacatcgt ccaccgtgac
    17461 gtaaacgccc caagccgacc gcatcccgtc gaccgcttcg ccaccgacgt tgaccatcgc
    17521 gtagccctcc ccgcccggat cgtctttggt ctgccagccg aaaagcgcac cataaaactg
    17581 tttggcggca gccacgtcgg tggtcatcag tttgggatga ggccgtgatg cctgatcatg
    17641 gat
//
LOCUS       DAORPM010000016        17190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_625_length_17190_cov_11.956989, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000016 DAORPM010000000
VERSION     DAORPM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 17190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17190
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_625_length_17190_cov_11.956989"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..82)
                     /gene="rpmB"
                     /locus_tag="PLD53_01600"
     CDS             complement(<1..82)
                     /gene="rpmB"
                     /locus_tag="PLD53_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRC59629.1"
                     /translation="MARVCQVTGKGPMVGNNVSHANNRTKR"
     gene            complement(181..858)
                     /gene="radC"
                     /locus_tag="PLD53_01605"
     CDS             complement(181..858)
                     /gene="radC"
                     /locus_tag="PLD53_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRC59630.1"
                     /translation="MAITDWPENERPRERLLAQGAAALTDAELLAIFLRVGMKGKSAV
                     DLARELIQHFGSLNRLFSATRDDFSAISGMGPAKYAQLQAVLEMSRRALAEEMKRSNA
                     FSTPAAVRDYLRLHLAGLAHEVFLALWLDAQNCLIAAEELFHGTLTQTSVYPREVVKK
                     ALWHNAAAVVLAHNHPSGVAEPSNADQLLTRELKQALALVDVRVLDHFIVAGQNQPLS
                     FAERGLL"
     gene            921..2111
                     /gene="coaBC"
                     /locus_tag="PLD53_01610"
     CDS             921..2111
                     /gene="coaBC"
                     /locus_tag="PLD53_01610"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRC59631.1"
                     /translation="MELKDRRIALGITGGIAAYKAAELVRLLIKEGATVQVVMTEAAT
                     HFVTPVTFQALSGRPVFSDQWDARMANNMAHIDLSREADVLLVAPASADFLAKLANGL
                     ADDLLSTLALARDCPLLAAPAMNRQMWENAATQRNIATLIDDGITLFGPDCGDQACGE
                     NGQGRMIEPAEIVARLIAHFQPKRLNGKKVLLTAGPTFEAIDPVRGITNLSSGKMGFA
                     LARAAQEAGAKVTLVSGPVSQPTPPGVNRIDVISALEMHTAVMQHAAAQDVFIGVAAV
                     ADYRPQAVAEHKIKKDGQSAPPIELVQNPDILADVAALPAPPLCVGFAAESRNLAEYA
                     EKKRMTKKIPLIVGNLIQHGFGGDDNTLTLFDADGATPLPPGSKLELARALIGHIAAL
                     VEGR"
     gene            2111..2317
                     /locus_tag="PLD53_01615"
     CDS             2111..2317
                     /locus_tag="PLD53_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59632.1"
                     /translation="MHSPHSDSANDYAREKTRYIVGRAALRRASRTIQDWQLEEQEKG
                     QLAKRIAIGLLIVALLGVALFILI"
     gene            2329..2778
                     /gene="dut"
                     /locus_tag="PLD53_01620"
     CDS             2329..2778
                     /gene="dut"
                     /locus_tag="PLD53_01620"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRC59633.1"
                     /translation="MHRIDVRLLDSRLKDNPPHYATPGSAGLDLRACIEAPVKIHPGD
                     TMLIPTGIAIHLADSGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGEIMVSTWNRG
                     KESFTLNPLDRLAQLVIVPVMQVAFDVVDEFTISTRGSGGFGSTGKS"
     gene            2817..3230
                     /locus_tag="PLD53_01625"
     CDS             2817..3230
                     /locus_tag="PLD53_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRC59634.1"
                     /translation="MSHQQTIETSTNGRGTTEITAEVARIVRASHIETGLVNVFVLHT
                     SCSVILTENADPTVRRDLETLALRWAPDGDSAYRHDSEGDDDMAAHGRNILAGSSITV
                     PVSNGDLKLGTWQGIYLWEHRTFSHRRRIVVTVIG"
     gene            complement(3266..5050)
                     /gene="pabB"
                     /locus_tag="PLD53_01630"
     CDS             complement(3266..5050)
                     /gene="pabB"
                     /locus_tag="PLD53_01630"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRC59635.1"
                     /translation="MIDGRFALFDSDNGHAILLSEYRRAIIFDGDDERRDDLASVFSA
                     FEAAARQGEWVALAADYALGGCFEPASTLPKTGRPLLRGWVFGQARQLDPAALEAFFS
                     EQLAGVSEQDRVVGVADLVAALDAEEHASKVEQIRRWIADGDCYQINLTFPIDFRLYG
                     HPLALYARLRERQPVRHGAYIDTQEEILLSFSPELFFERIGGRVLTRPMKGTTQRGAS
                     PSDDVVRRNELLASEKERAENIMIVDLLRNDLGKLALPGKVRVESLCEVEAYPTLWQV
                     VSTVSAELPDVCLYDLFRALFPCGSITGAPKIRAMQRISELEDSPRGIYTGSLGWMAP
                     NGDCRFNVAIRTLALAPDGRARLGVGSGVVIDSEPEREYAECLLKSQFLTGFDPGFKL
                     IETMRLEHGAYRLLDFHLERLKASAFALGFACDVSSIAASLSEQASAQTTGIFRVRLT
                     LSHNGHYLISLTSMDDGIQSWKVVVADERVDAENYLLRHKTSARGMYDRALARLAACP
                     DVFDAIFLNTREEVCEGARSNVFVERGGVLLTPPLSCGLLPGVMRRQLLETGRAVECV
                     LDRDDLLSASPIYLANSLRGLVSVSLQT"
     gene            complement(5112..6605)
                     /locus_tag="PLD53_01635"
     CDS             complement(5112..6605)
                     /locus_tag="PLD53_01635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRC59636.1"
                     /translation="MNIVNFCLSSSLFRIVRAVLQLTVAIAVSGFPNSWAAELPDLGE
                     SARADFSPQVERRIGESIMNEIRLREPSYVDDPEINDYLNRLGHRLVEASANPVGDFH
                     FFAIRDNTVNAFAMFGGFIGVNTGTILTAQSESELAGVIAHEIGHVTQNHLARQIAKE
                     KQNTIPSMIAMAVGLLAARANANIGMATISSVQAGVIQSQLGFSRDFEREADRSGYRT
                     LEKAGFDVRGMSDFFERMQKAGRVYENNAPVYLRTHPLTIERISDMQNRAQSSPYRQV
                     VSSLDFHLVRAKLRALMGTPREAVSEFETLLREKKYASQAAAQYGLAFAFMRAKDGPA
                     AQREMDALKALKVASPIIADLAAEIRAAAGDLPAAQDIYRDALLRFPQAKSLVYGYAE
                     SLYAGRQYEQALQFLDSQVQLSFSDYKLYGLQARSYSALGRRLQQHRAQAEFYFLQGQ
                     LGQAVEQLQFAQQATDGNFYEKSAADARLRELRKLLAEEAKLKKDGG"
     gene            complement(6754..6957)
                     /locus_tag="PLD53_01640"
     CDS             complement(6754..6957)
                     /locus_tag="PLD53_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRC59637.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGSEDLFAHFSAITMGGFKTLKE
                     GEKVSFDVTQGPKGKQASNIGRA"
     gene            7190..7498
                     /gene="clpS"
                     /locus_tag="PLD53_01645"
     CDS             7190..7498
                     /gene="clpS"
                     /locus_tag="PLD53_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRC59638.1"
                     /translation="MAKQQQEGSVLEAKRSKTAPPPLYTVLLLNDDFTPMEFVVLVLQ
                     RFFSMNREQAMQIMLKVHIEGRGVCGVYPRDVAATKVEQVAIFARENQHPLACVMEEN
                     "
     gene            7499..9757
                     /gene="clpA"
                     /locus_tag="PLD53_01650"
     CDS             7499..9757
                     /gene="clpA"
                     /locus_tag="PLD53_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HRC59639.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNTSAAETL
                     RACGGNIEQLRKDLTKFIVEHTPTVDGEDDIDTQPTLGFQRVIQRAILHVQSSGKKEV
                     NGANVLVAIFGEKDSHAAYYLQKQGIARLDVVNYISHGISKVPQGTVRSENDVETEGE
                     AQVAAGPLESYTINLNALAQQGKIDPLIGREKELERVIQTLCRRRKNNPLLAGEAGVG
                     KTAIAEGLARRIVENNVPEVLEKANVYALDMGALLAGTKYRGDFEQRLKGVLKQLSEK
                     PQAILFIDEIHTLIGAGSASGGTLDASNLLKPALSNGQLKCIGATTYTEYRGIFEKDH
                     ALSRRFQKIDVNEPSIAETIEILKGLKTRFETHHGIKYSAAALTSAVELSVRFISDRH
                     LPDKAIDVIDEAGAAQRILPKSRQKKMIGKQDIEEIVAKIARIPSTHVSTDDRSSLKN
                     LDRDLKAVVFGQEAAIDALTRAIKMARSGLGNPTKPIGCFLFSGPTGVGKTEVAKQLA
                     YCLGNELLRYDMSEYMERHAVSRLIGAPPGYVGFEQGGQLTEAVTKKPYCVLLLDEIE
                     KAHPDIYNILLQVMDHGTLTDNNGRKTDFRNAVIIMTTNAGQESIQKTSMGFTNVKTA
                     GDEMVEIKRLFTPEFRNRLDATISFKALDHDIILRVVDKFLIQLEEQLHEKKVEAAFT
                     DRLKEHLAVHGFDPLMGARPMARLIQDTIRSALADELLFGRLTSGGQVTVDLDEEDKV
                     RLVFEEEAEAVV"
     gene            9829..10323
                     /gene="rraA"
                     /locus_tag="PLD53_01655"
     CDS             9829..10323
                     /gene="rraA"
                     /locus_tag="PLD53_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HRC59640.1"
                     /translation="MSFKTPDLLDSNEEQTLAGAVRVVAPMFQRYGARTSFSGQIVTL
                     KLFEDNSLVREAFAEAGRGKVLVIDGGGSMRCALVGDQLAILAHKNGWEGAIVFGCIR
                     DSGDINLIDIGVRALNTHPLKSIKKGVGDRNIDITFGGVTFKPGEWIYADEDGVIVSS
                     SALT"
     gene            10573..11871
                     /gene="aceA"
                     /locus_tag="PLD53_01660"
     CDS             10573..11871
                     /gene="aceA"
                     /locus_tag="PLD53_01660"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRC59641.1"
                     /translation="MSTREQQIAELEKDWAENPRWKGIKRGYSAADVVRLRGSFPIEY
                     TIARQGAEKLWNLVNSEPYVNCLGALTGGQAMQQVKAGVKAIYLSGWQVAADNNGYAA
                     MYPDQSLYPVDSVPKVVERINNSFRRADEIQFSKNINAGDKGYVDYFAPIVADAEAGF
                     GGVLNAFELMKAMIRAGAGGVHWEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARFAA
                     DVCGVPTLVIARTDAEAADLLTSDYDENDKPFVTGERTAEGFYKTKKGLEQAISRAVA
                     YAAYADLVWCETGTPDLEFARKFAEGVHAKFPGKMLAYNCSPSFNWKKNLDDATIAKF
                     QKELGAMGYKYQFITLAGIHNMWYHMFDLSQDYVKRGMTAYVEKVQEPEFAARDRGYT
                     FVSHQQEVGTGYFDEVTTVIQGGVSSVTALTGSTEEEQFH"
     gene            complement(11966..12457)
                     /locus_tag="PLD53_01665"
     CDS             complement(11966..12457)
                     /locus_tag="PLD53_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRC59642.1"
                     /translation="MKTLLVVYNSMTGGTEQMVRALVMGAKLEDGVTTRLLHASAALA
                     ADVINADGYVFATPENLAAISGLMKDFFDRCYYPALNRINGRPYAVLVCAGSDGQNAV
                     RQIDRIATGWRLRRTAEALIVCTHAQSPETILSRKTISPPDLDRCRELGAAFATGLAI
                     GVF"
     gene            complement(12650..12737)
                     /locus_tag="PLD53_01670"
     tRNA            complement(12650..12737)
                     /locus_tag="PLD53_01670"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12701..12703),aa:Ser,seq:cga)
     gene            12773..14173
                     /gene="mltF"
                     /locus_tag="PLD53_01675"
     CDS             12773..14173
                     /gene="mltF"
                     /locus_tag="PLD53_01675"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HRC59643.1"
                     /translation="MRLILLLLAVIAGGCGDSTRQPLESTKKLVVLTRVGSTTYSVDE
                     TNGATGFDYDLVRMFAKELGLKIRIVVAASDSDILLRLKNGEAHMAAAWQSPVDDPEI
                     HSSTPYFQDYDVLVSHEAALPLTGIEQLANKTIHVVSGSRQEEALRELSSEFPEMIIV
                     GSHKRRELDLLEGVATQRLEATLVNNAAFNIGNNFYPELQDALKIDAARPIVWLFSPG
                     VDPELIARADAFLERTQKNGEMDRLKDRYFGHVKRLTEADSIRFIERMHSVLKPYRPL
                     FQEAQTRTGIDWRLLAAVAYQESQWDPLATSPTGVRGIMMLTEDTADLLGVSNRLDAA
                     QSISAGAQYIRDLREALPASIAEPDRLWLALAAYNLGMGHLNAARYIAKTLKANPDSW
                     YAMKKVLPLLGLPQYYSRLKSGKGRGGEAVIMVENIRVFTDILDRYEPAYNPLEHVSV
                     IGSRQQKLRTEQPVTP"
     gene            complement(14187..15473)
                     /gene="serS"
                     /locus_tag="PLD53_01680"
     CDS             complement(14187..15473)
                     /gene="serS"
                     /locus_tag="PLD53_01680"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRC59644.1"
                     /translation="MLDIQLLRNDIGVVSERLATRGYTLDIAAFQQLEAERKSLQTRT
                     QDLQASRNSLSKRIGVLKGKGEDASAVLAEVATLKEELDANEIRLDALLKTFDAFVAM
                     IPNLPQESVPVGKSEVDNVEVRRWGTPRNYDFTVRDHVDLGEALGLLDFATAAKIAGS
                     RFSLMTGSLARLHRAIAQFMLDVHSEEHGYTEVYAPYLVNAASLFGTGQLPKFEEDLF
                     KILRPDAEPLYLIPTAEVPVTNIVRDEILAWETLPLKLVCHTPCFRSEAGSYGKDTRG
                     MIRQHQFDKVELVQIVAADKSNEAHEELTRHAEIILEKLDLPYRRVALCTGDMGFSSA
                     KTYDLEVWLPAQNTYREISSCSNFESFQARRMQARCRNEKNKTELVHTLNGSGLAVGR
                     TLVAIMENYQNADGSISVPAVLRSYLGGLEKIAAQK"
     gene            complement(15475..16809)
                     /locus_tag="PLD53_01685"
     CDS             complement(15475..16809)
                     /locus_tag="PLD53_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRC59645.1"
                     /translation="MSDLFAQPHPVPLAEALRPATLDEVIGQQHLLGAGKPLRLAFEA
                     RKLHSLILWGPPGVGKTTLARLMADAFDAEFIALSAVFSGIKDIREAVASAEAAWARH
                     GRPTILFVDEVHRFNKAQQDAFLPYVESGLLTLVGATTENPSFEVNSALLSRASVYVL
                     NPLSEDELGVLLERACRQALQGLSFEDDARSRLIGAADGDARRLINLLEQMRTAAATA
                     NLSRIDSDLIENTLAQNLRRFDKGGEAFYDQISALHKAVRGSNPDAALYWFCRMLDGG
                     ADPRYLARRIVRMAWEDIGLADPRAAQITGSAAETYERLGSPEGELALAEAVLYLALA
                     AKSNAAYVAYNAARGFIAKDASRPVPLHLRNAPTRLMKELDYGKDYRYAHNEAEGFAA
                     GENYFPEGMPPVEWYRPVERGLEIRLGEKLAHLRDLDRDALKFTRTPKDSED"
     gene            complement(16802..>17190)
                     /locus_tag="PLD53_01690"
     CDS             complement(16802..>17190)
                     /locus_tag="PLD53_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HRC59646.1"
                     /translation="KIWIYDPDLRQVTVKKFDAALGSSPVALLVGESDGKGSDKGALE
                     RNFTLREAGEREGLEWLEAIPKSADSGFEKLLLGFSGNDLKSMELFDNFGQVTTLFFS
                     NLDRNPQLSPTLFRFTAPAGVDVLGE"
BASE COUNT         3903 a         5052 c         4754 g         3481 t
ORIGIN      
        1 gacgcttcgt tctattgttg gcatgggaaa cattgttccc gaccatcggg cctttgcccg
       61 tcacttggca gactcgcgcc atgattgttg ctccagttgt gcttgggatg aaggtcgggg
      121 attctagctc aaaacaaggg cttgcctcaa gttaattgtg cgatgcgatt tgtgagcggc
      181 ctatagcaac ccgcgttcgg caaacgacag gggctggttt tgtccggcca cgatgaagtg
      241 atccagaact cgcacgtcaa ccagggccag cgcctgcttg agctcccgcg tcagcaactg
      301 gtcggcgttg gaaggttcgg cgacgccgga gggatgattg tgcgccagaa ccaccgcagc
      361 agcgttgtgc cacagcgctt tcttgaccac ctcgcgcgga tagacactgg tttgcgtcag
      421 cgtgccgtgg aacagctctt cggcggcgat caggcagttt tgagcgtcca gccacagcgc
      481 gagaaagacc tcatgcgcca gcccggccag atgcaggcgc agataatcgc gcactgcagc
      541 cggcgtcgag aaagcattgc tccgcttcat ttcctcggcc agagcccggc gcgacatttc
      601 aagaacagcc tgcaactggg cgtatttggc tggccccatg ccggatatgg cggagaaatc
      661 gtctcgagtc gccgagaaca ggcgattcag gctgccgaaa tgctggatca actcgcgagc
      721 cagatcaacc gcactctttc ccttcatgcc gactcgcaga aagatagcca gcaattcagc
      781 gtcggtcagt gccgcggcgc cttgcgccag caagcgttca cgcgggcgtt cgttttccgg
      841 ccagtcggta attgccattt tgcaatctcg gttagaatgt tccgtctggc attctatcgc
      901 caagctctcg ggtcattcgc atggaattga aagatcgacg tattgctctc ggcatcaccg
      961 gcggaattgc tgcctacaag gcggccgaac tggttcgatt gctgatcaag gagggcgcca
     1021 ctgtgcaggt ggtaatgacc gaggcagcca cccacttcgt aacgcctgtc accttccagg
     1081 cgctctccgg cagacctgta ttcagcgacc aatgggatgc gcgtatggcc aacaacatgg
     1141 cgcacatcga tctgtcgcgc gaagccgacg ttctgctcgt cgcaccggct tcggccgatt
     1201 ttcttgccaa gctggccaac gggttggctg acgatctgct gagcacactt gctctggcac
     1261 gtgactgccc gctgctggcg gcaccggcga tgaaccggca gatgtgggag aacgcggcaa
     1321 cgcaacgcaa tatcgccacg ctgattgacg atggcattac cttatttggc ccggactgcg
     1381 gcgatcaggc ctgtggagag aacggccagg gtcgcatgat cgagccggca gaaatcgtgg
     1441 cccgattgat tgcccacttc cagcccaaac gactcaatgg caagaaggtt ctgctgaccg
     1501 ccgggccgac cttcgaagcc atcgatccgg ttcgcggcat caccaatctg tcgagcggaa
     1561 aaatgggttt tgccttggcc cgcgcggcgc aggaagccgg agccaaggtt accctggtca
     1621 gcggcccggt cagtcagccg acaccacccg gagtgaatcg catcgacgtc atcagcgcac
     1681 tcgaaatgca cactgccgtc atgcaacatg ccgccgcaca ggacgtattc atcggtgtcg
     1741 ctgcagtggc cgactaccgc ccgcaagccg ttgccgagca caaaatcaag aaagacggac
     1801 agagtgcgcc gcccattgaa ctggtccaga atccggatat tctggccgat gtcgctgccc
     1861 tgcccgcacc gccgctatgc gtcggtttcg ctgccgaaag ccggaatctg gcggaatacg
     1921 cggagaaaaa gcgcatgaca aagaagatac cgctgatcgt cggcaacctg attcaacacg
     1981 gcttcggcgg cgacgacaac accctgaccc tgttcgacgc agatggtgca accccgcttc
     2041 cgcctggatc gaaactggag ttggccaggg cgctcatcgg gcacattgcc gcgctggtcg
     2101 agggacgctg atgcattcgc cgcatagtga ctctgctaac gattacgcgc gcgagaaaac
     2161 ccgttacatc gtcggtcgtg cagcactcag acgcgccagc cggacgatcc aggactggca
     2221 gttggaagaa caggagaagg gtcagcttgc aaaacgcatt gccatcggcc tccttatcgt
     2281 tgcgcttctt ggcgttgccc tattcatcct catctgacag gaaacaacat gcatcgcatc
     2341 gacgttcgtc tcctcgactc tcgactcaag gacaacccgc cgcattacgc cactcccggt
     2401 tcagccggac tggatctacg cgcctgtatc gaggccccgg tgaaaatcca ccccggagac
     2461 accatgctga ttccgacggg catcgccatt catctcgccg actccggact ggcggcaatg
     2521 attctgccgc gttccggcct gggtcataaa cacggcattg tgctcggcaa tctggtggga
     2581 ttgatcgact ccgactacca gggcgaaatc atggtttcca cctggaatcg aggcaaagag
     2641 agcttcacac ttaatccgct ggaccgtctt gcgcaactcg ttatcgtccc ggtaatgcag
     2701 gttgcattcg acgtggttga cgagttcacc atcagcacgc gtggctccgg cggcttcggc
     2761 agcaccggaa aatcctgata atccattcag tcagcgatca ggcgaaggaa gaggacgtgt
     2821 cgcaccaaca gaccattgaa acatcaacaa acgggcgggg aacgacggaa atcaccgctg
     2881 aggtcgccag gatcgtccgc gcttcgcaca tcgaaaccgg tctggtcaat gtttttgttc
     2941 tgcatacgag ttgctcggta attcttaccg aaaatgccga tccgacggtg cgcagggacc
     3001 ttgaaacgct ggcgctgcgc tgggcgcccg acggcgactc cgcctatcga cacgacagcg
     3061 aaggtgatga cgacatggcg gcgcatggca gaaacattct ggcgggcagc tcaatcacgg
     3121 tgccggtaag caacggcgac ctcaagctgg gaacctggca aggtatctat ttgtgggaac
     3181 accggacttt ctcccatcgc cgaagaatcg tcgtgaccgt catcggctga cggttcggag
     3241 aagacctgtc accctcgccg acacttcagg tctgcaggct caccgaaacc agcccgcgca
     3301 agctgtttgc caggtagatc ggcgacgcac tgagcaagtc gtcccgatcc aggacgcact
     3361 cgacggcgcg ccccgtttca agaagctgcc ggcgcatgac gcccggaagc agtccacaag
     3421 acaacggcgg cgtcagcaga acgccaccgc gctctacaaa tacgttgctg cgcgcgccct
     3481 cgcacacttc ttcacgcgta ttgagaaaaa tcgcatcgaa cacatccggg catgcggcca
     3541 gccgggccag ggcccgatca tacatgccgc gcgcgctggt cttgtggcgc agaaggtagt
     3601 tctcggcatc cacgcgttcg tccgctacaa ctaccttcca tgattgaatg ccgtcatcca
     3661 tcgaagtaag gctgatcaga tagtggccgt tgtgggaaag agtcaaacga acccggaaaa
     3721 taccggttgt ctgagccgat gcctgctccg acagactcgc cgcaatcgac gagacatcac
     3781 acgcaaagcc cagggcaaac gccgaagcct taagccgctc cagatggaag tccagaagac
     3841 gatacgcccc gtgctccaaa cgcatggttt caatgagctt gaaaccaggg tcaaagcccg
     3901 tgagaaactg cgatttcagg aggcattcgg catattcgcg ttcgggctcc gaatcaatga
     3961 cgacgccgct accgacgccg aggcgagccc gcccatcagg ggcaagcgca agcgtccgaa
     4021 tcgctacatt gaaacgacaa tcgccgttag gcgccatcca gcccagcgac cccgtgtaga
     4081 tgccgcgcgg agagtcctcc aactccgaga tacgctgcat ggcgcgaatt ttcggagcgc
     4141 cggtaatcga accgcaagga aatagcgccc ggaacaagtc atagaggcac acatcgggca
     4201 gctctgccga aactgtcgat acgacctgcc acagagtcgg ataggcttca acttcgcaca
     4261 aggattcaac ccgcaccttg ccgggcaacg ccagcttccc cagatcgtta cgcagcaggt
     4321 cgacgatcat gatgttctcg gcgcgctcct tctccgacgc cagcaattca ttgcgcctca
     4381 caacatcgtc tgacggcgac gcaccacgtt gagtggttcc cttcatcggg cgcgtaagga
     4441 ctcgaccgcc gatccgctcg aaaaacagtt caggagaaaa ggaaagcagt atttcctctt
     4501 gcgtgtcgat gtaagctcca tgacgcaccg gctggcgttc gcgcagtcgg gcgtatagcg
     4561 ccagcggatg gccgtagagc ctgaaatcga tcgggaaggt gagattgatc tggtagcaat
     4621 cgccgtcggc aatccagcgc cttatctgct cgacctttga cgcatgttct tcagcatcga
     4681 gggccgccac aaggtcggcc acccccacaa cccgatcctg ttcggaaacg ccagccagct
     4741 gctcactgaa aaacgcttcg agcgccgccg ggtcaagctg ccgggcctga ccaaacaccc
     4801 aaccccgcaa caacgggcgc ccggttttcg gcaaggtgct ggcgggctcg aaacacccgc
     4861 caagcgcata gtccgcagca agagctaccc attcgccctg ccttgcagcc gcttcaaatg
     4921 cggaaaatac cgacgccaaa tcgtcgcggc gttcatcatc gccatcgaaa atgatcgctc
     4981 gtcgatactc cgacaaaaga atggcatgac cgttatcgct gtcaaacaat gcgaaacggc
     5041 catcgatcat gatcagcgtt cagtcttgaa gaaacggttc tgctgccggc aagctgttgc
     5101 tagatggatg ttcaaccgcc atcctttttc agcttcgcct cttcggccag caacttgcgc
     5161 aattcgcgga gtcgcgcatc agcggcagat ttttcataga aattgccatc ggttgcctgc
     5221 tgtgcgaact gcaactgctc aaccgcctgc ccgagctgcc cctgcaggaa atagaactcg
     5281 gcttgcgccc gatgctgctg caagcgcctg cccagggcag aatatgatct ggcttgcagg
     5341 ccatagagtt tgtagtcaga aaaactgagc tgcacttgtg aatccagaaa ctgcaaggcc
     5401 tgttcgtact gcctccccgc ataaagcgac tcggcatacc catagacgag cgacttggcc
     5461 tgaggaaagc gtaacagggc atcgcgatag atgtcctggg cagcaggcaa gtccccggcg
     5521 gccgccctga tctcggcagc cagatcggca ataattggcg acgctacctt gagcgctttg
     5581 agagcgtcca tttcacgctg cgcagccgga ccgtccttgg ccctcatgaa ggcaaaggcg
     5641 agcccgtact gagcggccgc ctgcgatgca tatttctttt cccgcaacag ggtttcaaac
     5701 tcactcacgg cctcgcgtgg cgtacccatc agcgcgcgca gcttggcgcg cacgagatga
     5761 aaatcgaggc tgctgacaac ctgccgatag ggcgagctct gcgcccggtt ctgcatatcg
     5821 gaaatccgtt cgatcgtcag cgggtgcgta cgcaaataga cgggcgcatt gttctcgtaa
     5881 actcgcccag ccttctgcat gcgttcgaag aaatcgctca tgccacgaac atcgaagccg
     5941 gccttctcga gggttctata gcccgagcga tccgcctccc gctcaaaatc ccggctgaac
     6001 ccaagctgcg attgaatcac gccggcctgc accgaagata ttgtggccat gccgatattt
     6061 gcattggcac gggcagcaag caggccaacg gccatggcga tcatgcttgg aatcgtattc
     6121 tgcttttcct tggcgatctg ccgggccaga tggttctgcg tcacgtgccc gatttcgtga
     6181 gcaatcacgc ccgccagttc cgactcggac tgtgctgtaa gaatggttcc ggtatttacg
     6241 ccgataaagc caccgaacat cgcgaacgcg tttaccgtgt tgtcgcgaat ggcaaaaaag
     6301 tggaaatcgc caaccggatt cgcactggct tcaacaagac ggtggcccag gcgattgagg
     6361 tagtcgttga tttccggatc gtcaacgtag ctgggttcac gcagtcgaat ctcgttcatg
     6421 atgctctcgc cgatccggcg ctcaacctgc ggcgaaaaat cggcacgcgc cgactctccg
     6481 agatccggga gctcagcggc ccatgaattc gggaatcccg agaccgctat tgccaccgtc
     6541 agctgcaaga cggcacggac tatgcggaat agcgagctgg aaaggcagaa gttcactatg
     6601 ttcatgaata caattccaag tacacggtgc aatgataccg gactcgatcg tatcgccgtg
     6661 tttccagatg tttcaggcgt gccctctcaa attcgctcga cgaaaaaaaa cccgccaaag
     6721 gcgggttttc tcggcgcagc tctgagacta agctcaggca cgcccaatat tcgaagcctg
     6781 cttgccctta ggaccctgcg taacgtcgaa ggaaaccttc tcgccctctt tgagggtctt
     6841 gaatccgccc atggtgatcg cggaaaaatg cgcaaaaaga tcttcgctgc catcatccgg
     6901 agtaatgaaa ccgaaaccct tggcgtcatt gaaccactta actgtacctg ttgccatatt
     6961 gctacttcct tgaaaagaga caagatccgg gcatcgcccg acgtgcatgt ttgaaattca
     7021 aattaggact tgaaccaaaa catgaagact ttttacgcta gttatggtgg atgcgtcaag
     7081 catttttgaa cgcgaatgcc ggcacagcgg gatttgtaca acgaaagagg caaacaaagc
     7141 ggttctaaag tggttttgca cattcgaaaa gactggttag aattgaatca tggcaaaaca
     7201 gcagcaggaa ggttccgtac tcgaggctaa acgcagcaag accgcgccgc cgccgcttta
     7261 caccgtactg ttgctcaacg acgatttcac gccaatggag tttgttgttc ttgtgttgca
     7321 gcggtttttt tccatgaaca gagaacaagc aatgcaaatc atgctcaaag tacatatcga
     7381 ggggcgaggc gtctgtggag tttatccgcg tgatgtggcc gccaccaagg tcgagcaggt
     7441 tgcgatcttc gccagggaaa accagcatcc cctggcgtgt gtaatggagg aaaactgaat
     7501 gattgcgcaa gagcttgaag tcagcctgca catggctttt gtcgaggcac gacaaaagcg
     7561 ccatgagttc atcacggtcg agcaccttct gcttgcgctg cttgacaaca cctcagccgc
     7621 cgagaccttg cgcgcctgtg gcggaaacat agaacagctt cgcaaggatt tgaccaagtt
     7681 catcgttgag cacacgccga cggtcgacgg tgaagacgat atcgatacgc aacccacgct
     7741 cggttttcag cgcgttatcc aacgcgccat cctgcatgtc cagtcttccg gtaagaaaga
     7801 ggtcaatggc gccaatgtcc tggtcgcgat ttttggcgaa aaggattcac acgcggcgta
     7861 ttacctgcag aaacagggaa tcgcccgcct tgatgtagtc aattacattt cccacggcat
     7921 cagcaaggta ccgcaaggca cggtgcgcag cgaaaatgat gttgaaaccg aaggtgaagc
     7981 ccaggtcgcc gccgggccgc tggagagcta caccatcaat ctcaacgcac ttgcccagca
     8041 aggcaagatc gatcctctga tcgggcgtga aaaggagctt gagcgggtca tccagacact
     8101 ctgccgtcga cgcaagaaca accccttgct ggcgggcgaa gcgggggtgg gcaaaactgc
     8161 cattgccgag ggccttgccc gtcgaatcgt tgaaaataac gtgccggagg ttctcgagaa
     8221 ggcaaacgtc tacgcactag acatgggcgc gctgctggcg ggcacgaagt atcgcggcga
     8281 cttcgagcag cgcttaaaag gcgttctgaa gcaactgagc gaaaaacccc aggccattct
     8341 gttcatcgac gaaatccata ccctgattgg tgccggatcg gcttccggcg gcacactcga
     8401 tgcatcgaac ctgctcaaac cggcgctttc caatggccag ctcaagtgca tcggtgcgac
     8461 aacctacacc gagtaccgcg gcatcttcga gaaagatcac gcgctgtcgc gtcgcttcca
     8521 gaaaatcgac gtcaatgagc cttccatcgc cgagaccatt gaaattctca agggtctgaa
     8581 aacccgcttc gagacgcacc atggcatcaa gtactcggcc gcagcgctga cctcggccgt
     8641 tgaactctcg gtacgcttca tctccgaccg ccacctcccg gacaaggcca tcgatgtcat
     8701 cgatgaagcc ggtgccgcgc aacgcattct tcccaaatcc cgtcagaaga agatgattgg
     8761 caagcaggac atcgaagaga tcgtggccaa gatcgcacgc attccctcga cgcacgtctc
     8821 gaccgatgac cgcagttcgc tgaagaacct tgaccgcgat ctcaaagcgg tagtctttgg
     8881 acaggaggca gccatcgatg ctctgaccag ggcgatcaag atggcacgtt cgggactggg
     8941 caatccaacc aagccgattg gctgcttcct gttttccggc cccactggcg tcggcaaaac
     9001 cgaagtggcc aaacagctcg cctactgcct cggcaatgaa ttattgcgtt atgacatgtc
     9061 cgaatacatg gagcgtcatg cggtttcaag gctgatcggt gcgccaccgg gctatgtcgg
     9121 tttcgagcag ggcgggcaat tgactgaggc agtcaccaag aagccttatt gcgtactgtt
     9181 gcttgacgag atcgaaaaag cccacccgga tatttacaac atcctgttgc aggtgatgga
     9241 ccatggcacc ctgacggaca acaacggccg caagactgat tttcgcaacg cggtcatcat
     9301 catgaccacc aatgccggtc aggaatccat tcaaaaaacc agtatgggct ttaccaatgt
     9361 caagacggca ggcgacgaga tggtcgaaat aaaacgactg ttcacgcccg agttccgcaa
     9421 ccggctcgac gccaccattt ccttcaaggc actcgaccac gacattattc tgcgcgttgt
     9481 cgacaagttc cttatccaac ttgaggaaca gttgcacgag aagaaagttg aagccgcttt
     9541 caccgatcgg ctaaaggaac acctcgccgt acacgggttt gatccactga tgggcgcacg
     9601 ccccatggcg cgcctgattc aggacaccat ccgctcggcg ctggctgacg aattgctgtt
     9661 cggacgcttg accagcggcg gtcaggtaac ggtcgatctg gacgaagagg acaaggtaag
     9721 actggttttt gaagaagaag ccgaagcggt cgtttgacta gcctggacaa ggcgctctcc
     9781 tcagggcttt cttatcgccc ccaagtattt actcattcag gattccccat gagtttcaaa
     9841 acccccgacc ttctcgattc caacgaagag cagacccttg caggcgccgt gcgtgttgtc
     9901 gctccaatgt tccagcgtta cggcgctcga acttcgttca gtggccagat cgtcacgctc
     9961 aagctgtttg aagataactc gctggtgcgt gaggcattcg ctgaagccgg aaggggcaag
    10021 gtgctggtaa ttgatggcgg cggttcgatg cgttgcgcct tggttggcga tcaattggct
    10081 atcctcgcgc acaagaacgg ttgggaaggc gcgatcgtct tcggctgtat ccgggattct
    10141 ggtgatatca acctgatcga cattggcgtt cgtgccctca atacccaccc tctgaaaagc
    10201 atcaagaagg gcgttggcga ccgcaacatc gacatcacct tcggcggtgt caccttcaag
    10261 ccgggcgaat ggatttacgc tgatgaggac ggcgtgatcg tctcatcgtc ggcgctgact
    10321 taagaccttc ttgcagcaat tcccgaacga tcgcctccaa cttgattcgg caggatcaag
    10381 tcgcacgcct gaccaaaaat tatcgcgagc ggcaaatatc ttatgtctta tataagacta
    10441 ttgatgctga agcaaaagcg ctctataatt gttcgtatag actgctcgga atagtgaatc
    10501 atgcatagac aagacttgca gtctgttttt ctcgaattcc ggtcatgttt ctaatctttt
    10561 gggagatcaa ttatgagcac acgtgaacaa caaatcgccg aacttgaaaa agactgggct
    10621 gaaaacccac gttggaaggg aatcaagcga ggctactcag cggctgatgt tgtccgtttg
    10681 cgtggctcct tcccgatcga atacaccata gcccgtcaag gcgccgagaa gttgtggaac
    10741 ctcgtcaaca gcgaacctta cgtcaattgc ctcggcgcat tgaccggagg ccaggccatg
    10801 caacaggtca aggcaggtgt aaaagccatc tacctgtccg gctggcaggt tgccgcggac
    10861 aacaacggtt atgccgcgat gtatcccgat cagtcgctgt accccgtcga ctccgtcccg
    10921 aaggttgtcg agcgcatcaa taactcattc cgtcgcgccg acgagattca gttctccaag
    10981 aacattaacg ctggagacaa aggctacgtt gattatttcg cgccgatcgt tgccgatgct
    11041 gaagccggat tcggcggcgt gctcaacgct ttcgagctga tgaaagccat gatccgcgct
    11101 ggcgctggcg gcgttcactg ggaagaccag ctggcctcgg tcaagaagtg cggccatatg
    11161 ggcggcaagg ttctggtgcc aacccaggaa gcggtccaga aactgattgc cgcacgtttt
    11221 gctgctgacg tctgtggcgt accgacgctg gttatcgccc gtaccgatgc cgaagctgcc
    11281 gacctgctga cctccgacta tgacgagaac gacaagccct tcgttaccgg cgagcgcaca
    11341 gcggaaggct tttacaagac caaaaaaggc ctggagcagg ctatctcccg cgccgtggcc
    11401 tatgcggcct acgccgacct cgtatggtgc gaaacgggca caccggatct ggagtttgcc
    11461 cggaaattcg ccgagggcgt acatgccaag ttcccgggga agatgcttgc ctacaactgc
    11521 tcgccttcgt tcaactggaa gaagaatctg gacgacgcca ccatcgccaa attccagaaa
    11581 gaactcggcg ccatgggcta caagtaccag ttcatcaccc tcgccggcat ccacaacatg
    11641 tggtaccaca tgttcgatct gtcgcaagac tatgtaaaac gcggcatgac ggcctacgtc
    11701 gaaaaggttc aggaacccga gtttgcggcg cgtgatcgtg gttacacctt cgtatcgcac
    11761 cagcaggaag tcggtaccgg ctatttcgac gaagtcacca ccgttatcca gggcggagtc
    11821 tcatcggtca ccgcactaac cggctcgaca gaagaagagc agtttcactg atctgactcg
    11881 atcaaaaaag ccgccagcag gcggcttttt tttgccccaa cgccacgctt gcttcggttt
    11941 gtggacggcg gaattgtccg tgcgatcaga acacgcctat cgctagtccc gtagcgaacg
    12001 ctgctccaag ttccctgcag cgatcaagat caggcgggct gatggttttg cgcgaaagta
    12061 tggtttccgg agattgcgcg tgtgtgcaaa cgatcagagc ttcggctgta cgtcgcagtc
    12121 gccatccggt agcgatgcgg tcaatctgtc gcacggcgtt ttgcccatcg ctgcctgcac
    12181 agaccaggac ggcataaggc cgaccgttga tccgattgag cgcagggtaa tagcagcgat
    12241 caaagaaatc cttcatcagc cccgaaatag ccgcgagatt ctccggcgtt gcgaacacgt
    12301 aaccgtcggc gttgatgaca tccgccgcca aagcggccga tgcatggagt agtcgcgtcg
    12361 tcactccatc ttcaagcttg gcacccatga ccagcgcacg caccatttgc tcggtgccgc
    12421 cggtcatcga gttatataca acgagtagcg ttttcatctc gggtgcccgc ataaaagggg
    12481 atcacacgtc tcgccggatc cacagaagaa gaacagcccg tcgatagtgc atgaccgagt
    12541 tcttgtgtca ggatagacca ttcaagcgca caatgaacgg tatctgcgat ggcaaataat
    12601 acggcaaagc acttcgtgcg gcgcttggat acgaaggtgg cttggcatct ggcggagagg
    12661 gagggattcg aaccctcggt accttgcagt acgcctgatt tcgagtcagg tacaatcgac
    12721 cactctgcca cctctccgtc aggggcggga gtttagcaca actcgacagc ctatgcgcct
    12781 gatccttctt cttctcgccg tcattgctgg cggctgcggc gatagcactc ggcagcctct
    12841 cgaaagtacc aaaaagcttg tcgtcctcac ccgagtgggt tcaaccacct actcggttga
    12901 tgaaacgaat ggcgccacgg gctttgacta cgacctggtc cgcatgttcg ccaaggaact
    12961 gggtctgaag atccgtattg ttgttgccgc cagcgacagt gacatactgc tacgactcaa
    13021 aaacggcgaa gcgcacatgg cagccgcgtg gcagtcgccg gttgacgatc cggagattca
    13081 cagcagcacg ccttacttcc aggactatga cgtgctggtc tcccacgaag cggcattgcc
    13141 attgaccggc atcgagcaac tggccaacaa gaccatacat gttgtttccg gttcgcggca
    13201 ggaggaggcg ttacgcgaac tgagtagtga atttccagaa atgatcatcg tcggcagtca
    13261 taaacgccgt gagctcgact tgctggaagg cgtcgcaacc caacgcttgg aggcgaccct
    13321 agtcaataac gcagcattca atattgggaa caatttctac ccggaattgc aggacgcgct
    13381 gaagatcgac gcagcccgac cgatagtctg gctgttttca ccgggtgttg atcctgaact
    13441 gattgcccga gccgacgctt ttctggagcg cacgcagaag aatggcgaaa tggatcgctt
    13501 gaaagaccgt tatttcgggc atgtcaagcg gctcaccgag gccgacagca ttcgtttcat
    13561 cgagcgcatg cactctgttc tcaagcccta tcgccccctt tttcaagagg ctcagacgcg
    13621 aacaggaatc gactggcggc tgctggcggc ggtcgcctac caggaatccc agtgggatcc
    13681 gctggcgacc agtccaaccg gcgtgcgcgg cataatgatg ctgaccgagg atactgccga
    13741 cctgctgggc gtcagcaacc ggctcgatgc agcgcaaagc atcagtgccg gagcccagta
    13801 catccgtgac ctgcgcgagg cgctcccggc gagcattgcc gaacccgatc gtttatggct
    13861 ggcgcttgcc gcctacaatc tgggcatggg tcacctgaac gccgcccgtt acattgccaa
    13921 gacgctaaag gccaacccgg attcctggta tgccatgaag aaggtactcc ccttgctcgg
    13981 acttcctcag tactacagcc ggctaaaatc cggcaagggg cgtggcggag aagccgtcat
    14041 catggttgag aacatccgcg tcttcaccga catacttgat cgctacgaac cagcttacaa
    14101 tccattggaa cacgtatctg tcattggaag caggcaacag aaactgcgaa cagaacaacc
    14161 ggtaacgcca taacgatccg agcggcttac ttttgcgccg cgattttttc caggccgcca
    14221 agatacgaac gcaagacagc gggcacggaa atgctgccgt cggcattctg gtagttctcc
    14281 atgatggcga ccaaggtgcg cccaacggca aggcccgaac cattgagcgt gtgcaccagc
    14341 tcggtcttgt ttttttcatt tcggcagcgc gcctgcatgc gccgcgcctg gaaggactcg
    14401 aaattggagc aggaagaaat ttcgcggtag gtattctgtg ccggcagcca gacttccaga
    14461 tcgtaagtct tggccgaaga gaagcccata tcgccggtgc atagggcgac gcgacgataa
    14521 ggcaaatcaa gtttctcaag aatgatctcg gcgtgacgcg tcaactcctc atgcgcctca
    14581 tttgatttgt cagctgcaac aatctgcacc agttcaacct tgtcgaactg gtgctgacga
    14641 atcatgccgc gcgtgtcctt gccgtaggaa cctgcctccg agcgaaaaca cggtgtatgg
    14701 cagaccagtt tgagcggcag agtttcccaa gccagaattt cgtcgcgcac gatgttggtg
    14761 accggaactt cggcggtcgg aatcagatac agcggctcgg catcaggacg caatatcttg
    14821 aacagatcct cctcgaattt cggcaactgg ccggtgccga acaggctggc ggcattgacc
    14881 aggtagggtg catagacttc ggtatagccg tgctcttcgc tatgcacgtc gagcatgaac
    14941 tgggcaatgg cgcgatgcag gcgggcaagc gacccggtca tcagcgagaa acgcgagccg
    15001 gcaatcttgg ccgccgtggc aaaatcgagc agcccaagtg cttcgcccag atcgacgtga
    15061 tctctgaccg tgaaatcgta gttgcgcggt gtcccccaac gcctcacctc gacgttgtcg
    15121 acttcggatt tgccgaccgg aacactctcc tgaggtagat tgggaatcat cgccacaaag
    15181 gcgtcgaagg tcttgagcaa ggcgtcgagc cggatctcgt tggcatcgag ttcttccttc
    15241 agcgtggcga cttcggccaa gacggccgaa gcgtcctccc ccttgccctt gagcacgcca
    15301 atccgcttgg acaggctgtt acggctggcc tgtaagtcct gggtgcgcgt ctgcagcgac
    15361 ttgcgttcgg cttcgagttg ctggaatgcg gcaatgtcaa gggtataacc tcgtgttgcc
    15421 aaccgttcgg aaacaacacc tatatcgtta cgcaacagtt gaatatcaag catttcagtc
    15481 ctctgagtct ttgggagttc ttgtgaattt cagcgcatcg cggtcaagat cgcgcagatg
    15541 agcaagcttt tcgcccaatc tgatctccag cccacgctcg accggccggt accattcgac
    15601 cggtggcata ccttccggaa aatagttttc gccggcggca aagccctcgg cttcattgtg
    15661 cgcgtatcgg taatccttgc cgtagtcgag ttccttcatc agccgcgtcg gcgcattgcg
    15721 caagtgaagc ggcaccggcc gggaagcatc tttggcaatg aatcctcgcg ccgcattata
    15781 cgcgacgtag gccgcattcg atttggctgc cagcgccaga tagagcacgg cttcggccag
    15841 cgccagttcg ccctcgggtg agccgaggcg ttcgtaggtt tcggctgcgc tgccagtaat
    15901 ttgtgccgca cgcgggtcgg cgaggccgat gtcttcccac gccatgcgca cgattcgtcg
    15961 cgccagatag cgcggatctg cgcccccgtc gagcatgcgg cagaaccagt acagcgccgc
    16021 atcgggattg gaaccgcgca ccgccttgtg cagagccgaa atctggtcgt aaaaggcctc
    16081 gccgcctttg tcgaagcggc gcagattctg cgccagtgtg ttttcgatca agtcgctgtc
    16141 gattcgcgac agattcgctg tggccgccgc agtacgcatc tgctccagca gattgatcag
    16201 ccggcgcgca tcaccgtcgg ccgcgccgat caagcgcgat cgcgcatcgt cctcaaaact
    16261 caacccttgc agggcctgcc ggcaggcgcg ctcaagcaac acgcccaatt catcttcgga
    16321 caggggattc agaacataaa ccgaagcgcg cgacaggagg gcagaattga cttcgaacga
    16381 ggggttctcg gtcgtcgcac cgaccagcgt cagcaggccg ctttcaacgt aaggcagaaa
    16441 ggcatcctgt tgcgccttgt tgaagcgatg gacttcatca acaaaaagta tcgtcggtcg
    16501 accgtgccga gcccatgcgg cttcagcact cgctaccgcc tcccgaatat ccttgatccc
    16561 cgaaaatacc gcagacaggg caatgaattc ggcatcaaag gcatcggcca tcaggcgtgc
    16621 caacgtcgtt ttgccgacgc ctggcggtcc ccagagaatc agcgaatgca gtttgcgcgc
    16681 ttcaaacgcc agcctgagag gcttcccggc gccgagcaga tgttgctggc cgatcacctc
    16741 gtccagcgtc gcgggtctca gcgcttccgc gaggggcact ggatgaggct gtgcgaagag
    16801 atcactcacc taggacatcg acgcccgcag gggccgtaaa gcggaaaagc gtgggcgata
    16861 gctgtggatt acggtccaga tttgaaaaga acaaggtcgt gacttgtccg aagttgtcga
    16921 acagctccat cgacttgaga tcgtttccgg agaaccccag caacaatttc tcaaaaccac
    16981 tgtccgctga tttcgggatc gcctccagcc attcgagacc ctcgcgctcg ccggcttcgc
    17041 gcagggtaaa gtttctctca agggcacctt tgtcgctccc cttaccatca ctctcgccaa
    17101 caagcagcgc caccggcgag ctgccaaggg cggcgtcgaa cttcttcacc gtcacctggc
    17161 gcaaatcagg atcgtagatc catattttct
//
LOCUS       DAORPM010000017        16185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_689_length_16185_cov_11.704154, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000017 DAORPM010000000
VERSION     DAORPM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 16185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16185
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_689_length_16185_cov_11.704154"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLD53_01695"
     CDS             <1..788
                     /locus_tag="PLD53_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC59647.1"
                     /translation="HEWIMGELGLPVIDNYWQTETGWPILSAMPGVEKTEIRFGTPSF
                     PVYGYDLRIFREDGTICDPDEKGIVAIVPPLPPGCLSTVWGDDDRFVSTYFTLFKEPQ
                     VYSSFDWGIKDKDGYHYILGRTDDVINVAGHRLGTREIEEAIQAHPAIAEVAVVGVAD
                     KLKGQMPMAFAVVKDAASIATPELRTALEKAVFKVVDDNLGAIARPSRVHFLSVLPKT
                     RSGKLLRRSIQALAEGRDPGDLTTIEDPAALEQLQQALGNKAA"
     gene            989..1381
                     /gene="rpsF"
                     /locus_tag="PLD53_01700"
     CDS             989..1381
                     /gene="rpsF"
                     /locus_tag="PLD53_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRC59648.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKALVATHGGQMHRLEDWGR
                     RQLAYPIQKLHKAHYVLMNIECNGEALAELEHGFKFNDAVLRHLTIKMKKAVTTPSPM
                     MKEEKSKSMMETREDVADDASTETESLA"
     gene            1396..1704
                     /gene="priB"
                     /locus_tag="PLD53_01705"
     CDS             1396..1704
                     /gene="priB"
                     /locus_tag="PLD53_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HRC59649.1"
                     /translation="MNRVELTGILIERKALRFTPAAVPVTECVIRHQSDQFEAGCPRR
                     VECEIRAIGLGEAARWLQAATPGVTVQLTGFLAARSQNSKQLRLHVTMIEFVEGNQNG
                     "
     gene            1697..1972
                     /gene="rpsR"
                     /locus_tag="PLD53_01710"
     CDS             1697..1972
                     /gene="rpsR"
                     /locus_tag="PLD53_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRC59650.1"
                     /translation="MGRFFKKKDDKNVKKRGSGLFKRRKFCRFSLEKMDGIDYKDVDL
                     FKEYIAENAKIMPARLTGTKAGYQRLLSVAIKRARFLALLPYTDNHQ"
     gene            1987..2436
                     /gene="rplI"
                     /locus_tag="PLD53_01715"
     CDS             1987..2436
                     /gene="rplI"
                     /locus_tag="PLD53_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRC59651.1"
                     /translation="MQIILMEKVVNLGNLGDLVNVKHGYARNFLIPQGKAKRATPLAM
                     KEFEGRRAELEKIAAEKLAAAQAFAEKLEGVLVSVARKAGVDGRLFGSVGNHDIADGL
                     KALGFDVEKSDIRMPMGPLKTVGESSLEISLHTDVLASIKVAVVVEQ"
     gene            2577..3980
                     /gene="dnaB"
                     /locus_tag="PLD53_01720"
     CDS             2577..3980
                     /gene="dnaB"
                     /locus_tag="PLD53_01720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRC59652.1"
                     /translation="MAKPSNSAHSLNSPIDPAIAHLRVPPHSIEAEQSVLGGLLLDNA
                     AFDKIADLVGESDFYRDEHKRIYRQIRKLLERNKPVDVVTVAESLDLSGEGGETGGLA
                     YLGELAANTPSAANIRRYAEIVRERAVLRQLVTAGDEIAGSALNPLGRDPKQLLDEAE
                     ARVFAIAEGGFRHQTGFQHINPLLTQVVERIQELHDRDNPSDITGVPTGYHDLDSKTS
                     GLQPGDLLIIAGRPSMGKTSLALNMAEYVAIEVGLPVAVFSMEMGGTQLAMRMLSSVG
                     RLDAHRVRTGRLNDDEWSRLSFALGKMHEAPLYIDETPALNPIDLRARARRLHRQCGK
                     LGMIVIDYLQLMSVSGQGENRATEISEISRSLKSLAKELNVPVIALSQLNRSLEQRPN
                     KRPVMSDLRESGAIEQDADVIMFIYRDEVYNPDTPDKGSAEIIIGKQRNGPIGMTRLT
                     FVGEYTRFENFASPGTY"
     gene            complement(4057..5889)
                     /locus_tag="PLD53_01725"
     CDS             complement(4057..5889)
                     /locus_tag="PLD53_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRC59653.1"
                     /translation="MTGDILLVLGLLLATILLFASDRLRMDIVAILVLLALMMSGLLA
                     PKEALAGFGDPLVVLIAGLFVIGEGLFRTGVAFAIGNWLLGVAGNSETRLLVLLMLVV
                     AGLSAFMSNTGAVAVFIPVALNLSTKANVPANRLLMPMAFSGSLGGMLTLIGTPPNLV
                     VSNLLSREGMTPFNFFSFTPVGLLILVVTIIFIVLIGRFLLPKDGGPKGNAQDRLSLE
                     DLISAYGVKDQLFRLCIESDSPLVGQTLGQVQLRTRFDVIVIGLERQSRGKPVVMPAL
                     IQTEYSAGDIIYLAGTEQRVAELLRTLAGMRRLTFEAQQKQIVAQELGLAEVLLTPRS
                     RLVGQTIKDAHFRERYGLSVLGILRMGQPMRGDFLATRLDFGDSLLVGGGWRHIDLLQ
                     AEQTDFSVLTIPREMDEVAPYRERAPWALAIVAGMLGLMTFDILPSVTAVLLAGLAMV
                     LARCVTMEEAYKSINWQSIILIAGMLPMATALNKTGALELIVTGLVESLGTLGPIALM
                     AGLFVLTSVFSQFISNTATAVLVAPIALGAATSMELSPYPFLMAVALAASTAFSTPMA
                     SPVNTLVLGPGGYRFNDFVKMGVPLQLLAMALTLLVVPLIFPFR"
     gene            complement(6058..7251)
                     /locus_tag="PLD53_01730"
     CDS             complement(6058..7251)
                     /locus_tag="PLD53_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRC59654.1"
                     /translation="MKRRDFLATSAILSLLAASPADAAKKAAAKKPAAKSTSRSAKGK
                     PPAAATSAEHAAPQTETHNVISLPEEPAAQWRTYDILSTIALKQVNGKARLWLPLAQY
                     KDTPWERSLGHNWQGNFENAGIYRDPVAEMEVFYADWTEGIANPQLQLTSQVATQDRH
                     FDITRRGAVAERTEVLRRCLQSTKLVPNDGIVRTTAERAIGRIKDPVARGKAIYDWVV
                     ENATYDPQMKSVGNSNIGSMLESNNLSGRSADIALLFVGLCRSIGIPARPVFGLRTDS
                     SRLFGSLGAGGNLSTDQHCRAEFYTPGYGWIPVEPSDVRKAILDERLSNGDPKLTVLK
                     KLLFGFWEMNWISFNVAQDVSLRGSIGQSLPFLVYPVVETVDGRFDSLDTNRVGFQVS
                     ANRIE"
     gene            complement(7361..8788)
                     /locus_tag="PLD53_01735"
     CDS             complement(7361..8788)
                     /locus_tag="PLD53_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRC59655.1"
                     /translation="MAIRTAARAKTKKASPLTKLFVLDTNVLLHDPTSVYRFEEHDVF
                     LPIMTLEELDNNKKGLSEVSRNARQVTRTLDEIVSSHSQGIEIGIALAGPSHKLASGR
                     LFLQTEAIANDLPIGLPTAKADNQILAVVIHLQRANPEREVILVSKDINMRIKACALG
                     LEAQDYFNDKVLEDTDLLYTGVLELPANFWDKHSQDMESWKRDGLTLYRVNGPLCPQL
                     LVNEFIYQEGEQALYAIVREVSGKMAQFETLVDFTHPKNSIWGITARNREQNFALNLL
                     MDPEVDFVSLLGQAGTGKTLLTLAAALTQVLEDKLYSEIIMTRVTVPVGEDIGFLPGT
                     EEEKMGPWMGALEDNLEVLNKTDEEAGEWGRAATHDLIRNRIKVKSLNFMRGRTFLNK
                     FLIIDEAQNLTPKQMKTLITRAGPGTKVVCMGNIAQIDTPYLTEGSSGLTYVVDRFKG
                     WKHSGHVTLQRGERSRLADYAGEVL"
     gene            complement(8835..9290)
                     /locus_tag="PLD53_01740"
     CDS             complement(8835..9290)
                     /locus_tag="PLD53_01740"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRC59656.1"
                     /translation="MIDTIISDFELPSTGDQPFRLSDCRSGMIVIYFYPKDSTPGCTT
                     EAQQFRDLYPGFVKAGCEVLGVSRDSIRSHQNFKTKQNLPFELLSDADERLCTQFSVI
                     KMKTMYGKQVRGIERSTFVIDGKRTLRREWRGVKAEGHAQEVLDFIRTL"
     gene            9607..11013
                     /gene="leuC"
                     /locus_tag="PLD53_01745"
     CDS             9607..11013
                     /gene="leuC"
                     /locus_tag="PLD53_01745"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRC59657.1"
                     /translation="MLQNLYDKLWQSHVVHEESDGTALIYIDRHLVHEVTSPQAFEGL
                     KLAGRKPWRISSIVATADHNTPTNHWERGIEDPVSRLQVETLDANIREVGALAYFPFK
                     DLRQGVVHVVGPENGVTLPGMTVVCGDSHTSTHGAFGCMALGIGTSEVEHVLATQCLL
                     QKKSKTMLFRVDGQLGKGVTAKDIALAIIGRIGTAGGTGYAVEFGGSTIRGLSMEARM
                     TLCNMTIEAGARMGFIAVDELTIDYLKGRPYAPKAEQWDAAVTYWRTLHSDEGAEFDC
                     VLDMRAEDIQPQVTWGTSPEMVVSIGDSVPDPVSEKDPARREGMERALHYMGLKANTP
                     IKEIAIDKVFIGSCTNSRIEDLRAAASVLKGRKVSDSVKLALAVPGSGLVKQQAELEG
                     LDKIFVAAGFEWREAGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPV
                     MAAAAAIAGHFADVRDLL"
     gene            11025..11153
                     /locus_tag="PLD53_01750"
     CDS             11025..11153
                     /locus_tag="PLD53_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HRC59658.1"
                     /translation="MKILAFIFVAIISAMSVSACNTVQGVGKDIEKGGQAIEKAAK"
     gene            11174..11830
                     /gene="leuD"
                     /locus_tag="PLD53_01755"
     CDS             11174..11830
                     /gene="leuD"
                     /locus_tag="PLD53_01755"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRC59659.1"
                     /translation="MDKFDHLEGLVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFD
                     ELRYTDVGQPGEDCTDRPLNPDFVLNLPRYQGAQILLTRKNFGCGSSREHAPWALLDF
                     GFRVIIAESFADIFFNNCFKNGILPIVLPAAEIEALFAEVMATPGYTLSIDLTRQVVV
                     CPDGRELSFAVDAFRKECLLNGWDDIGLTLHHAEKIRAFEDKRRIEQPWLFEAGAGGV
                     "
     gene            11844..12131
                     /locus_tag="PLD53_01760"
     CDS             11844..12131
                     /locus_tag="PLD53_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59660.1"
                     /translation="MLPKIIVFLSSHFVATFLVMGLAFVGFGLTSLNLLYLIRANVEL
                     VLQHGAMALLDGALRQFFELIASGFLGLAMYLVFKVCEHILVEKLVHKHGE"
     gene            12136..13200
                     /gene="leuB"
                     /locus_tag="PLD53_01765"
     CDS             12136..13200
                     /gene="leuB"
                     /locus_tag="PLD53_01765"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRC59661.1"
                     /translation="MKICVLPGDGIGPEIMAEALNVLEALDLKFEIEEAQLGGCAVDA
                     TGNPYPEATQKLAQAADAVLLGAVGGLKWDTLPREQRPERGLLGIRKQLGLFANLRPA
                     ILYPELANASTLKPEVVAGLDILIIRELTGDIYFGQPRGIEMREVDGKNQRFGFNTMH
                     YTESEIRRIGQVAFEAARKRNRKVCSVDKMNVLETTQLWRDVMNELAPEYPDVELSHL
                     LVDNAAMQLVKAPKQFDVMVTGNMFGDILSDEASMLTGSIGMLPSASLDENNKGLYEP
                     CHGSAPDIAGMGVANPLATILSVAMMLRYTFAMEDVAQRIEGAVKKVLALGYRTGDIY
                     ETGTKKVGTREMGKAVLAAL"
     gene            13245..14375
                     /gene="asd"
                     /locus_tag="PLD53_01770"
     CDS             13245..14375
                     /gene="asd"
                     /locus_tag="PLD53_01770"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRC59662.1"
                     /translation="MKKLGLVGWRGMVGSVLLQRMAEERDFDHIEPVYFSTSAVGGKA
                     PSPGGGDGAVLQDAMSIDALKQTDIIITCQGGEYTKAVFPRLRAAGWNGHWIDAASTL
                     RMEKDAVIILDPVNLDVIHSALAAGGRNWIGGNCTVSLMLMGLGGLFKNDLVEWISAM
                     TYQAASGAGAQNMRELLAQMGSIHDAIKPDLADPASAILDIDRKVAETMRAASFPTSN
                     FRNTALAGSLIPWIDAPVEQGQSKEEWKGGAESNKILGRPAFRTPGSIPIDGLCVRIG
                     AMRCHSQGLTIKLRKDIPLDEVIGIIANGNQWVKVVPNEREASERELTPAAVTGTLTV
                     PVGRLHKLAMGTEYLGAFTVGDQLLWGAAEPLRRMLRILLEN"
     gene            complement(14395..>14820)
                     /locus_tag="PLD53_01775"
     CDS             complement(14395..>14820)
                     /locus_tag="PLD53_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC59663.1"
                     /translation="VSPSQIEIEITENVCIRNPQYAIEQLNKLCQLGVSIAIDDFGTG
                     YSSLSYLHRFPVHTVKIDQSFVKEIQRENGHFPVILAIISIASGLGLKLVAEGVETKV
                     QAHYLEKSGCKTMQGFLYYKPLSHTQFLELLREKRAISA"
     assembly_gap    14821..14920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14921..16158)
                     /locus_tag="PLD53_01780"
     CDS             complement(<14921..16158)
                     /locus_tag="PLD53_01780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRC59664.1"
                     /translation="MDFINAKGIDTKVIVLSGDTGIEAAIGALKRGAYDYLRKPYTRE
                     ELLKTIDNALQQRRLESENRSIASRLESSEKLYRYLVDSSPDIIYTLDQEGRFTFVND
                     RVQQLLGYGPKDLVGRHYSVLVHDEDIERARYVFNERRVGERASRNVELRLKSLTGSD
                     QERTFDNTLMTISFNSMGMYSLGKEVVDRKFFGTYGVARDISDRKRAEELISYQAYHD
                     ILTDLPNRILFKDRLGLAVIQARRNTSELAVMFVDLDRFKLVNDTLGHVKGDELLQQV
                     AERLRNCLRRGDTLARIGGDEFTVVLPELRDRQDAEIIADKFLDSLRQPFALDSKEVH
                     ISASIGIAVYPSDGESIDELLRHADVAMYNVKAQGKNAHSFYESSMLDASYQKVALEQ
                     SLRRALEQGELEMYYQPQVDV"
BASE COUNT         3474 a         4323 c         4714 g         3574 t
ORIGIN      
        1 cgcacgagtg gatcatgggc gaactcggct tgccggtgat cgacaactac tggcaaaccg
       61 agaccggctg gccgatactc tcggcgatgc cgggtgtcga gaaaaccgag atccgtttcg
      121 gtacgcccag cttcccggtt tacggttacg acctcaggat tttccgcgag gacggcacca
      181 tctgcgatcc ggatgaaaag gggattgtcg ccatcgtgcc accgctgcca cccggttgtc
      241 tctcgacggt ctggggcgac gatgaccgct tcgtttcgac ctacttcacg ctgttcaagg
      301 agccgcaggt ttactcctcg ttcgactggg gcatcaagga caaagacggt tatcactaca
      361 ttcttggccg taccgacgat gtcatcaacg tggcgggaca tcgtctcgga acgcgcgaga
      421 tcgaagaggc gatccaggct catcccgcta tcgccgaagt ggctgtagtc ggtgtcgctg
      481 acaagctgaa gggccagatg ccgatggcct tcgcggtggt caaggatgca gcatcgatag
      541 caactccgga gcttcgcacg gcgcttgaga aggcggtatt taaagtagtg gacgacaacc
      601 tcggtgccat tgcccgcccg agtcgtgtgc acttcttgag cgtgctgccc aaaacacggt
      661 cgggcaagct gctgcgtcgt tccattcagg cgttggcgga agggcgtgat ccaggcgatc
      721 tgacgacgat tgaagatccg gcagcgctcg aacagctcca acaggcgctg ggtaacaaag
      781 ctgcttgatg cacagcggat gacgtgaaat ttcgcgttat cccgaccagc cggtctgact
      841 gactccagtc tgatcggctg gtcggggctc tgtattggcc gggatacaaa tcgtatggat
      901 gtttaaaccg tagatcggct ataatgcccg gttttccacc ttgctccacc tttcgcatga
      961 aggggggctt taaccatagg gagtgttcat gcgtcattat gaaatagttt ttatcgtcca
     1021 cccggaccag agtgaacagg ttccggcgat ggtcgagcgt tacaaggctc ttgtagccac
     1081 ccatggaggg caaatgcacc gtctcgaaga ttgggggcgc cgtcagcttg cttacccgat
     1141 tcagaagctg cataaggctc actatgttct gatgaacatt gagtgcaatg gtgaagcgct
     1201 cgctgaactg gagcatggct tcaagttcaa cgatgccgta cttcgtcacc ttaccatcaa
     1261 gatgaagaag gccgtaacca cgccatcacc gatgatgaag gaagagaaat ccaagtcgat
     1321 gatggaaact cgggaagacg ttgcagatga cgccagcacc gaaaccgagt cgcttgcctg
     1381 aatgttgagg ctcagctgaa ccgcgttgaa ctcaccggaa tattgatcga gcgcaaggcg
     1441 ctacgcttta ctccagccgc ggtcccggtc acggaatgcg tgatcagaca ccagtcagat
     1501 cagttcgagg cgggatgtcc aaggcgtgtt gagtgcgaaa tccgggctat tggtctgggt
     1561 gaggcggcca gatggctaca ggcagcgaca ccgggagtaa cggttcaatt gacgggcttt
     1621 ctcgctgcca ggagccaaaa cagcaaacag ctgagattgc acgtaaccat gatcgaattt
     1681 gtagaaggaa atcaaaatgg gtagattctt caagaagaag gatgacaaga acgtcaagaa
     1741 acgtggcagt ggcctgttca agcgccgcaa attctgccgc ttcagtctcg aaaagatgga
     1801 tggaatcgac tacaaggatg tcgatctgtt caaggagtat atcgctgaga atgccaagat
     1861 catgccggct cgtctgaccg gtaccaaggc gggttaccag cggttgctgt cggtggcgat
     1921 caagcgcgca cgctttctgg ccttgttgcc ttataccgac aatcaccaat aatcgaggaa
     1981 aagacgatgc aaataattct gatggagaag gtcgtcaatc tcggaaacct cggcgatctg
     2041 gtcaatgtca agcatggcta tgcccgtaat ttcctgattc cgcaaggcaa agccaagcga
     2101 gccaccccgc tggcgatgaa ggaattcgaa ggcaggcgtg ccgagcttga aaagatcgcg
     2161 gcggaaaaac tcgcggccgc ccaggcattt gccgaaaagc tggaaggggt cttggtctct
     2221 gttgcacgca aggctggcgt tgacggccgc ttgtttggct ccgtgggtaa tcatgacatt
     2281 gcagacggac tcaaggcgct tggtttcgat gttgagaagt ctgatattcg catgccgatg
     2341 ggtccgctca agacggtagg cgagtcctcg ttggaaatct cgttgcacac cgacgtgttg
     2401 gcaagcatca aggttgccgt ggttgtcgag cagtaacgga agcaagcggc ccggttctcg
     2461 tccgctgaaa agccttgccc tggtgcaagg ctttttttat ttcagtgcat cgtggtatgc
     2521 gttgtccgga gtaaactcac cttcctgcct gtctcgcttt ccgtaccgga acttttatgg
     2581 ccaagccgtc caattcagct cattcactga actcaccgat cgacccggcg attgcgcatt
     2641 tgcgcgttcc ccctcactcg attgaggccg agcaatcggt gcttggtgga ttgttgctcg
     2701 ataacgcggc gttcgacaag attgccgacc tggtcggcga gagcgatttc tatcgcgatg
     2761 aacacaagcg catctaccgt caaatccgca agctgctgga acgcaacaaa ccggtcgacg
     2821 tggttacagt agcagagagt cttgatctgt ccggagaggg tggcgagacc ggtggcttgg
     2881 cttatctcgg tgaactcgca gccaatacgc cgtcggcggc gaatatccgg cgctacgcag
     2941 aaattgttcg cgaacgggcg gttctacgcc agttggtcac ggctggcgac gagatcgccg
     3001 gaagcgcgct caacccgctc ggccgcgatc cgaagcaact gcttgacgag gccgaagcca
     3061 gggtgtttgc catcgccgag ggtggcttcc gccatcagac cggtttccag cacatcaacc
     3121 cgctgctgac gcaggtggtc gaacgcattc aggaactcca cgatcgtgac aatccatcgg
     3181 atatcaccgg ggtgccgacc ggctaccacg atcttgattc gaagacctcg ggtctgcagc
     3241 ccggcgatct cttgatcatc gccggtcgtc cgtcaatggg caagacctcg ctcgcgctga
     3301 atatggccga atacgtggcg attgaagtcg gtttgcccgt tgccgtcttc tcaatggaga
     3361 tgggcggtac gcagttggcc atgcgcatgc tttcctccgt cggccggctt gatgcacatc
     3421 gcgtacgcac cggccgcctc aatgacgacg agtggtcgcg cctgtctttt gcgttgggca
     3481 agatgcacga ggcgccgctc tacatcgacg agacgccggc gctcaacccg atcgacctgc
     3541 gtgcgcgtgc acgcaggctg catcggcagt gcggcaaact cggcatgatc gtaatcgact
     3601 acctgcaact gatgtcagtc tccggacagg gtgaaaacag ggcgaccgag atctcggaaa
     3661 tctcgcgctc gctgaagagt ctggccaagg agcttaatgt tccggtgatt gcgttgtcgc
     3721 aactcaaccg ctcgctggag cagcgcccta acaagcgtcc cgtgatgtcc gacctgcgcg
     3781 aatcgggtgc catcgagcag gacgccgacg tgatcatgtt catctaccgc gacgaagtct
     3841 acaatcccga cacgccggac aagggcagcg ccgaaatcat catcggcaag cagcggaatg
     3901 gcccgatcgg catgacgcgc ctgacctttg tcggagagta cacgcgcttc gagaactttg
     3961 ccagtccagg cacgtactga cgcttgattc tgtttctcat ggtcgaagtt cctgtaccca
     4021 cgcttgcaca ttgaagtctg gatactcaat aagggattac cgaaacggaa aaatcagcgg
     4081 tacgaccagc aaggtcagcg ccatggccag gagctgcaga gggacaccca ttttcacgaa
     4141 gtcattgaat cggtagcctc caggtccaag aacgagagtg tttaccggtg aggccatggg
     4201 ggtggagaaa gccgttgacg ccgcgagcgc cacggccatc aggaaggggt agggagagag
     4261 ttccatactc gtcgccgcac ccagcgcaat gggtgccacc aggactgccg tcgcagtgtt
     4321 ggaaatgaac tgactgaaaa ccgacgtcag cacaaacaga ccggccatca acgctatcgg
     4381 ccccagcgtt cccaggctct ctaccaggcc ggtgacaatg agctccagcg cgcccgtctt
     4441 gttgagtgcg gtggccatcg gcagcatgcc cgcgatgaga atgatgcttt gccagttgat
     4501 cgacttgtac gcttcttcca tggtgacgca tcgtgcaagg accatggcca ggcctgccag
     4561 gagtacggcc gtaaccgacg gcaagatgtc gaatgtcatc aggcccagca ttccggcgac
     4621 gatagccagt gcccagggcg cgcgttcccg gtaaggtgcc acctcgtcca tctcacgagg
     4681 aatggtcaga accgagaagt cggtttgctc ggcctggagg agatcgatgt gccgccaacc
     4741 gccgccaacc agaagcgaat cgccaaaatc caggcgtgta gcgagaaaat cacctctcat
     4801 gggttggccc atgcgcagaa tacccagcac cgacaggccg taacgttcgc ggaaatgggc
     4861 atccttgatg gtctggccga caagccggga acgcggggtc agcaagactt ctgccaagcc
     4921 gagttcctga gcaacaatct gtttttgctg cgcctcgaag gtcaggcgcc gcattccggc
     4981 caacgtacgc agtagctcgg ccacccgttg ctctgtcccg gccagataga ttatgtcgcc
     5041 ggcactgtac tcggtctgga taagtgctgg cataaccacc ggcttacctc gcgattgtcg
     5101 ctccaggccg atgacgatga cgtcaaaccg ggttcgcagt tgtacctgtc ccagggtttg
     5161 cccgaccaga ggggaatcgg actcgatgca cagccggaat agctggtctt tgaccccgta
     5221 ggcgctgatc agatcttcta gcgagaggcg gtcctgggca ttgcccttcg gaccgccatc
     5281 ctttggcagc agaaagcgcc cgatcagaac gatgaaaatg atcgtgacga cgagaatgag
     5341 caagccgacc ggtgtgaagc tgaagaagtt aaagggcgtc atgccctccc tggaaagcag
     5401 attgctgacg accagatttg gcggggtgcc gatcaaggtc aacatgccgc cgagagatcc
     5461 agagaatgcc ataggcatta gcaggcgatt ggcgggcacg ttcgccttgg tgctcaggtt
     5521 cagtgcgacg ggaatgaaga cggcaacggc tccggtgttg ctcatgaatg cggagagacc
     5581 ggccacgacc agcatcaaca acaccaacag acgggtttcg ctgttgccgg cgacgccaag
     5641 caaccaattg ccaatggcaa aagcgacgcc ggtacggaac aaaccctcac cgatgacaaa
     5701 caatccggcg atcaatacca ccagtggatc accaaatccg gcgagagctt cctttggcgc
     5761 gagtagtccg ctcatcatca aggccagaag caccagtatg gcgacgatat ccattcgcag
     5821 ccggtcgctg gcgaatagca gtatcgtggc caggagcaag ccaagaacca gcaggatatc
     5881 acccgtcatg aagtctccga aggcttggaa agtctgccgc tttccgaagg cgtgaggact
     5941 gcgccggata gcaatgccgc agtcctggat tgcgccgtag accacggttc cgccaaaagc
     6001 gaacgtgcag tttgtgtccg gcccacacct gctatgctca ctccacttac agggtttcta
     6061 ttcaattcgg ttggcgctga cctgaaagcc aacacgattg gtgtccaggc tgtcgaatcg
     6121 accgtccact gtttccacga cagggtaaac caggaatggc agtgattgcc cgattgatcc
     6181 acgcaagctc acatcctggg cgacattgaa actgatccag ttcatttccc agaaaccgaa
     6241 aagcaacttc ttcaacacgg tcagttttgg gtcgccgtta ctcagccgtt catcaagaat
     6301 ggctttgcgt acgtcggagg gttcgactgg aatccagcca taacccggcg tgtagaattc
     6361 ggctcggcag tgttgatcgg tgctcaggtt gccgccggca ccgaggctgc cgaatagtcg
     6421 cgagctgtct gttcgcaggc cgaataccgg gcgcgccgga atcccgatgg atcggcacag
     6481 accgacgaag agcagggcga tgtcagcact tcggccactc aggttgttgc tctcaagcat
     6541 gcttccgatg ttgctgttcc cgacgctctt catctgtggg tcgtacgtgg cattttccac
     6601 gacccaatca tagatggcct taccccgggc aaccgggtcc ttgatgcggc cgatggcgcg
     6661 ttcggccgta gtccggacaa tgccgtcgtt gggtacgagt tttgtcgact gcaggcagcg
     6721 ccgaaggact tccgtgcgtt ccgccacagc gcctcggcgg gtaatgtcga aatggcgatc
     6781 ctgcgttgca acctggctgg tcaattgcag ttgcggattg gcaatgccct ccgtccagtc
     6841 ggcatagaag acttccattt ctgccaccgg atcacgatag atgccggcat tctcaaaatt
     6901 cccctgccag ttgtgaccca gagatcgctc ccacggggtg tctttgtact gggctagcgg
     6961 gagccagaga cgtgccttgc cgttgacctg tttgagggca atcgtcgaaa ggatgtcata
     7021 ggttcgccat tgtgccgctg gttcctcggg caggctgatg acgttgtgcg tttcagtttg
     7081 cggcgccgcg tgttcggcag aggttgccgc tgccggtggt ttcccttttg ccgaccttga
     7141 cgtggatttt gcagccggct tttttgccgc tgcctttttt gctgcatctg ccggcgaggc
     7201 agccagcagc gagagaatcg ccgaagttgc gaggaagtct cgtcgtttca atcgtttctc
     7261 cggaaggcgt tggatccatg cagtgcacta aacacacaag aacctgttgg caaaaacgac
     7321 cgagccgtgt cccgtgacac ggctcggtat ggcgaaaaaa ttataacact tcgccggcgt
     7381 agtcggcgag gcgggagcgc tctccgcgct gaagggtgac atgcccggaa tgcttccagc
     7441 ctttgaagcg atccacgaca taggtcaggc cagaactgcc ctcggtcagg tagggagtgt
     7501 cgatttgtgc gatgtttccc atgcagacga ctttggttcc cggcccggcg cgcgtgatca
     7561 gggttttcat ctgctttggt gtcaggttct gcgcttcgtc gatgatcagg aacttgttca
     7621 ggaaggtacg tccgcgcatg aagtttagcg acttcacctt gatccggttg cgaatcaggt
     7681 cgtgagtcgc cgcgcgcccc cattcgccgg cctcctcatc cgtcttgttg agtacctcaa
     7741 ggttgtcttc aagtgcgccc atccacggcc ccattttctc ttcttccgtg cccggcagaa
     7801 aaccgatatc ttcgccaacc gggaccgtta cacgggtcat gatgatttcg gagtagagct
     7861 tgtcttccaa gacttgcgtc agtgcggcgg caagggtcaa tagggttttc ccggtaccgg
     7921 cctggccaag cagactgaca aagtcaacct cagggtccat cagcagattg agagcgaagt
     7981 tctgttctcg gttacgggca gtgatccccc agatactgtt tttcgggtgc gtaaaatcga
     8041 ccagcgtctc gaattgcgcc atcttgccgg acacttcacg gacgatggca tacagagcct
     8101 gctcgccttc ctgatagatg aactcattga ccagaagttg cgggcagagc ggtccgttta
     8161 cgcgatagag cgtcaagccg tcgcgtttcc aggattccat gtcttggctg tgcttgtccc
     8221 agaagttggc gggcaattca aggacgccgg tatatagcag gtcggtgtct tccagcacct
     8281 tgtcattgaa ataatcctgg gcctccaaac caagcgcgca agccttgatg cgcatgttga
     8341 tatctttcga taccagaatg acttcgcgct ccgggttcgc acgttgcagg tggataacga
     8401 cagccagaat ctggttgtcg gctttggccg tgggcaaacc gataggcaaa tcgttggcga
     8461 tggcttcagt ctgaaggaag agacgcccgc tagctaactt gtgcgaaggg ccggcgagtg
     8521 cgatgccgat ctcgatcccc tgggagtggc tgctgacgat ttcgtccagc gtacgagtga
     8581 cttgccttgc attgcgcgag acttcggaca ggcccttctt gttgttatca agttcttcca
     8641 acgtcatgat cggcagaaag acatcgtgct cttcaaatcg atagacgctc gtcgggtcat
     8701 gcaacagcac attggtgtca agaacgaaga gcttggtcag cggtgaggct ttctttgttt
     8761 ttgcgcgggc ggcagttcta atagccatgg acgggctttc tccggtgaat acggggtggg
     8821 ggcattccgg ctgatcaaag cgttctaatg aaatccagca cctcctgcgc gtggccttcc
     8881 gccttgacgc cgcgccactc ccgccgaagc gttcgcttgc catcaatgac gaacgtgctg
     8941 cgctcgattc cacgaacctg ttttccgtac atcgttttca tcttgatcac ggagaattgc
     9001 gtgcacaacc gttcgtcggc atcggagagc agctcaaagg gtaggttctg tttggttttg
     9061 aaattctggt gcgaccggat gctgtcccgc gaaacgccaa gcacttcgca tccagctttt
     9121 acaaaaccag ggtagagatc ccgaaactgc tgagcctcag tggtgcaacc cggtgtgctg
     9181 tccttggggt aaaaataaat gacgatcatc ccgcttcggc agtcagaaag cctgaagggt
     9241 tggtcaccag tggacggcaa ctcgaagtcg ctgataattg tgtcgatcat gtttcgcgaa
     9301 cctatttatt gttagcccga ttgttgtaag aattccgcag ttggtcaagc tggacagcga
     9361 gaatgcgcca acgctgactc atgcacctga ctttcgcgag cgaggcgctc cccgaaagcc
     9421 tctgatggtc tctgattgtg gcgcagaatc attgccgggc cgcgtcgtcc atcttttgga
     9481 tcactccttg gcaggctgtg gtttctagcc attcaaggtc ttgcgagctg acagtaaggt
     9541 cgagccatga gataatccag cgttcaatcg ggccgttcgg gacccgactt ccaggataac
     9601 agaccgatgc tgcagaattt atacgacaaa ctttggcaga gtcatgtagt gcatgaggaa
     9661 tccgacggga cggcgctgat ttatatcgat cgccacctgg tgcatgaggt gaccagtccg
     9721 caagctttcg aggggctgaa actcgccggt cgcaagcctt ggcgcatttc ctccattgtt
     9781 gccactgctg accacaatac gccgaccaac cattgggaac gcggtatcga ggatccggtt
     9841 tcgcgtttgc aggtggaaac gcttgacgcc aatattcgcg aagtcggtgc cttggcctac
     9901 tttccattca aggatttgcg tcagggcgtt gttcatgtgg tcggcccgga aaatggcgta
     9961 acgttacccg gcatgaccgt cgtttgcggc gactcccaca ccagtaccca cggcgcattc
    10021 ggatgcatgg cgctgggtat cgggacgtcc gaagtcgaac acgttttggc cacgcagtgc
    10081 ctgttgcaga agaagtcgaa aaccatgttg tttcgtgttg acgggcagct tggcaagggc
    10141 gtgaccgcca aggacatcgc tctggccatc atcggtcgta tcggcacggc gggcggtacg
    10201 ggatatgctg tcgagtttgg tggcagtacg atacgcggcc tgtcgatgga agctcgtatg
    10261 acgctgtgta acatgaccat cgaggctggc gcgcggatgg gctttatcgc cgtcgatgag
    10321 ctgacaatcg attacctgaa aggtcgtcca tatgcgccga aggcagagca gtgggacgct
    10381 gcggttacct attggcgcac gcttcacagc gacgaaggcg ccgaattcga ttgcgttctc
    10441 gacatgcgtg ctgaagacat tcaaccgcag gtcacctggg gaacatcgcc cgaaatggtg
    10501 gtttcaatcg gggacagtgt tcccgatcca gtcagcgaaa aggatccggc gcgccgtgag
    10561 gggatggagc gagccctgca ttacatgggg ctgaaggcga atacgccgat caaagagatt
    10621 gccatcgaca aggtctttat tggctcgtgt accaactcgc gcattgagga tttgcgtgca
    10681 gcagccagtg tcctcaaggg gcgcaaagtg tctgacagcg ttaaattggc gctggctgtt
    10741 cccggttccg gtctggtcaa gcagcaggca gagcttgagg gactggacaa gatttttgtg
    10801 gccgccggct tcgagtggcg agaggcgggg tgttcgatgt gtctggcgat gaatgccgac
    10861 cggctggaac cgggagagcg ttgcgcctcg acttcgaatc gcaatttcga gggcagacag
    10921 ggtgcgggtg gacgaacaca tctcgttagc ccggtaatgg cagccgctgc agcgattgca
    10981 gggcattttg ccgatgttcg tgacttactt tagggagact gatcatgaag attctcgctt
    11041 tcatttttgt tgccatcatt tcggcaatgt cggtttctgc atgcaatacc gttcagggcg
    11101 tgggcaagga tattgaaaag ggcggccagg ccatcgaaaa ggccgccaag taaggtggat
    11161 cacaaggaaa accatggaca agtttgatca cctggaaggg ctggttgcac cgctggatcg
    11221 cgccaacgtt gacaccgatg caatcattcc gaaacagttt ctgaagtcaa tcaagcgctc
    11281 gggctttggc ccgaacctgt ttgacgaact gcgttacacc gatgtcggcc agcctgggga
    11341 agactgcacg gatcgtccgt tgaatccgga ttttgtgctc aacttgccgc gctaccaggg
    11401 tgcgcaaatt ctgctgacgc gcaagaactt cggctgcggc agctcgcgcg aacatgcccc
    11461 gtgggctttg ctcgatttcg gttttcgggt gattatcgcc gagagctttg cagacatttt
    11521 cttcaacaat tgcttcaaga atggcattct gccgatcgtt ctgccggcgg ctgaaattga
    11581 agcacttttt gccgaggtta tggccacgcc gggctacacg ctgtcaattg atctaacgcg
    11641 gcaagttgtc gtttgtccgg atggcagaga gctttcattt gcggtggacg cgtttcgcaa
    11701 agagtgtctg ctcaacggct gggacgacat tggactgacg ctgcatcacg ccgagaaaat
    11761 tcgtgcgttt gaggacaagc gtcgcattga gcaaccctgg ttgttcgaag cgggagcagg
    11821 tggtgtgtaa agcggcaaaa cgattgctgc ctaaaataat cgttttcctc agttctcatt
    11881 tcgtagctac tttcctagtc atggggctgg cttttgttgg cttcgggctc acatcgctca
    11941 acctgctcta tctgattcgt gccaatgtcg aactggtgct tcagcacggt gccatggctc
    12001 tgcttgatgg cgccctgcga cagtttttcg agttaatcgc ctcggggttc ttgggactgg
    12061 ccatgtatct tgtattcaaa gtttgtgagc atattctggt cgaaaaattg gtacacaagc
    12121 atggggaata aagtaatgaa aatttgtgtt ttgcccggtg acggcatagg accggaaata
    12181 atggccgagg ctctgaacgt gctggaagcg ctcgacttga agtttgagat cgaggaggct
    12241 cagcttgggg ggtgcgccgt cgatgcaacg ggcaacccgt atccggaagc gacccagaag
    12301 ctggcgcagg ctgcggatgc cgtcctgctt ggcgctgtcg gcggtctgaa atgggacacg
    12361 ctgccgcgcg agcaacgtcc ggagcgtggc cttctcggca ttcgcaaaca gctcggtctg
    12421 tttgcgaatt tgcgcccggc cattctctac ccggaacttg ccaatgcatc tacactcaaa
    12481 cccgaagtgg ttgctggtct cgacattctg atcatccgtg aactgaccgg agatatttac
    12541 ttcggtcaac cacgcggtat cgagatgcgc gaagtggacg gcaaaaatca gcgcttcggc
    12601 ttcaatacca tgcattacac ggagagcgag attcggcgca ttggacaggt cgcgttcgaa
    12661 gccgcgcgca agcgcaacag gaaggtgtgc tcggtagaca agatgaatgt tctggaaacg
    12721 acacaactct ggcgcgatgt gatgaacgag ctggcgcccg agtatccgga tgtcgagctg
    12781 tcacatctgt tggtggacaa cgcggcgatg caactggtga aggcacccaa gcagttcgac
    12841 gtgatggtca ccggcaatat gtttggcgac attctgtcgg acgaggcatc gatgctcacc
    12901 gggtcaatcg gcatgctccc atcggcctcg ctcgacgaga acaacaaggg tttgtacgag
    12961 ccttgtcacg gctcggcacc ggacattgcc ggcatgggcg tggccaatcc gctggcgacg
    13021 atattgtcgg tggcgatgat gttgcgctat acctttgcca tggaagatgt tgcgcaacga
    13081 atcgaaggcg cggttaaaaa ggtgctggcg ctcgggtatc gtaccggtga catttacgag
    13141 acgggtacga agaaagtagg tacccgtgaa atgggcaagg cagttctggc ggcattgtga
    13201 ttttggtgga tcaatcggtc tgatgtttat ttgaggcaag ggaaatgaag aagcttggcc
    13261 tagttggctg gcggggcatg gtcggttcgg tgctcctgca acgcatggcg gaagagcgtg
    13321 atttcgatca catcgagcca gtatatttct cgacttcggc ggttggcggc aaggcccctt
    13381 cccccggtgg aggtgatggc gccgtgttgc aggacgccat gtcgattgac gcgctcaaac
    13441 agacggatat catcatcacc tgtcagggtg gagagtacac caaggcggtg tttcccaggc
    13501 tgcgtgccgc aggctggaac ggccactgga tcgacgcggc ctccactttg cgcatggaga
    13561 aggatgccgt aatcattctt gacccagtca acctcgacgt cattcactct gccctggctg
    13621 cgggcggaag aaactggatt ggcggaaact gcacggtgtc gctgatgctg atgggtctcg
    13681 gcggactgtt caagaatgac ctggttgaat ggatcagcgc gatgacctac caggcagctt
    13741 ccggtgcggg tgcgcaaaac atgcgcgagc tgcttgcgca aatgggttcg attcatgacg
    13801 ccatcaagcc cgatctggct gatcctgctt cggccattct cgacatcgac cgcaaagttg
    13861 ccgagacgat gcgtgctgcg agtttcccga ccagcaactt tcgcaatacg gcactcgcgg
    13921 gtagcttgat tccgtggatc gacgcacccg tcgaacaggg gcagtcgaag gaggaatgga
    13981 agggcggtgc cgaaagtaac aagattctcg gtcgccccgc gtttcgcacg cccggcagta
    14041 ttccgattga cggtctgtgc gtgcgcatcg gcgccatgcg gtgccattcg caaggtctaa
    14101 ccatcaagct caggaaggat attccgcttg atgaggttat cgggattatc gccaatggca
    14161 atcagtgggt gaaggtggtg cccaacgaac gcgaggccag cgagcgtgag ttgacgccgg
    14221 cagctgttac cggaacgctc accgtgccgg ttggccgttt gcacaagctt gccatgggta
    14281 cggaatatct gggcgctttc actgttggcg accagttgct gtggggggcc gccgagccgc
    14341 tacgccgtat gctgcgtatc ttgcttgaaa actgatcgaa agttgagcag tctgtcaggc
    14401 actgatcgcg cgcttttccc gcagcagttc aaggaactgt gtgtgggaaa gcggcttgta
    14461 gtagagaaat ccctgcattg tcttgcagcc tgacttctca aggtagtgcg cctgaacttt
    14521 tgtttcgacc ccttccgcga caagtttgag tcccaggccg ctggcaatcg aaataattgc
    14581 cagaataacc ggaaagtgtc cgttctcgcg ctggatttcc ttgacaaagg attggtcgat
    14641 cttgaccgtg tgtaccggga agcgatgcaa atacgatagt gacgaatagc ctgtgccgaa
    14701 gtcatcaatg gcaatgctga caccaagctg gcatagtttg ttgagttgct cgatagcgta
    14761 ctgtggattg cgaatgcaaa cgttctcggt aatctcgatc tcgatctgcg atggcgaaac
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acatccacct ggggctggta
    14941 gtacatttcc agttcaccct gctcaagcgc ccggcgcagg ctctgttcca acgcaacttt
    15001 ttgatacgat gcgtcgagca tcgaagactc ataaaatgaa tgggcattct tgccctgtgc
    15061 cttcacgtta tacatggcga catcggcgtg acgcaacaat tcgtcaatcg attcaccgtc
    15121 ggacggatag acggcgatac cgatgcttgc cgagatatga acttccttgc tatccagtgc
    15181 gaagggctgg cgcagactat cgagaaattt gtcggcaatg atctcggcat cctgcctgtc
    15241 ccgtaattcc ggcaagacta cagtgaactc gtcaccgccg atacgcgcca gcgtgtcgcc
    15301 ccgtcgcagg cagtttctca ggcgttccgc aacctgctgc agcaattcgt cgcccttgac
    15361 gtggcccagg gtatcgttga cgagcttgaa gcgatcaagg tcgacaaaca tgaccgccag
    15421 ctcggaggtg ttgcgtctgg cttgaatcac ggccaggccg agtcgatcct tgaagagaat
    15481 gcggttcggc agatcggtca ggatgtcgtg ataagcctga taagagatca gctcttcagc
    15541 ccgtttgcga tcagaaatgt ctcgcgcaac cccataggtt ccaaagaact tgcgatcaac
    15601 cacctctttg cccaatgaat acattcccat cgagttgaac gagatggtca tcaatgtatt
    15661 gtcgaaagtg cgttcctggt cgctaccggt aagacttttc aggcgcaact ctacgttccg
    15721 cgaagcacgc tcgcctacgc gacgttcatt gaatacatat cgagcacgtt cgatatcctc
    15781 atcgtggacc aaaaccgaat agtgcctgcc gaccaaatcc ttcgggccat aaccgagtaa
    15841 ctgctgcacg cgatcattga cgaatgtgaa tcgcccctcc tggtcaagcg tataaatgat
    15901 atccggcgag ctatcgacga ggtagcggta aagcttttcc gagctctcaa gtcgcgaggc
    15961 aatgctgcga ttctcgctct caagtcgccg ttgctgcagc gcgttgtcga tcgttttgag
    16021 caactcttcg cgtgtgtaag gcttgcgcag atagtcatag gcgccacgtt tcaaagcgcc
    16081 aatcgccgct tcgattccgg tatcgccact gagtacaatc accttggtgt cgatcccctt
    16141 ggcattgatg aaatccatga tttcatggcc accgatgtcg ggcag
//
LOCUS       DAORPM010000018        15971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_706_length_15971_cov_9.418698, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000018 DAORPM010000000
VERSION     DAORPM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 15971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15971
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_706_length_15971_cov_9.418698"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLD53_01785"
     CDS             complement(<1..183)
                     /locus_tag="PLD53_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59665.1"
                     /translation="MGIVPNQDAMMKKILLVDDDPELRQLLATYLGRHGYDTLLLPDT
                     RQLDAFLERYQPHLVVL"
     gene            374..895
                     /locus_tag="PLD53_01790"
     CDS             374..895
                     /locus_tag="PLD53_01790"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HRC59666.1"
                     /translation="MNERYSKMIPRRIAGTLAAVAVSATMATAAFAMPWGGDCDGKSG
                     DRTECRSERGMKGMSRLHDELKLDAKQEALWQDAEKASKENMAGMRDRFRQHHEEIQA
                     LIDKPGADLRAVAKRMDEFKAEGQKLREANRDRWLTVYDSLNAEQKEKARVFFKSKLE
                     RMARHGHRGPGRS"
     gene            1068..1757
                     /locus_tag="PLD53_01795"
     CDS             1068..1757
                     /locus_tag="PLD53_01795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC59667.1"
                     /translation="MPRSIRRIVISGVTRGLGRAMLDEFVSLGHTVAGCGRSEAAIDD
                     LRLKFAAPHRFATVDVADDFQVGQWARHVLTTMGPPDILINNAALTNKPAPLWEVSAA
                     EFDALIDVNVKGVANMLRHFVPAMIAGERGVIVNFSSGWGRSTAPDVAPYCASKWAIE
                     GLTGSLAQELPEGMAAVTMNPGIIDTDMLRLSFGETAGGYEAPSAWAERAVPLLLKLG
                     PKNNGKALNIP"
     gene            1926..3068
                     /locus_tag="PLD53_01800"
     CDS             1926..3068
                     /locus_tag="PLD53_01800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRC59668.1"
                     /translation="MLKFRSLLIALLVSFLVPSLALAQQMPTPPALAAKSWLLLELES
                     GQELASQAPDERIEPASLTKLMTAYLTFAALKQGTIKLDQMVTVSENAWKSQGSRMFI
                     QVDTQVEIEDLIKGMIVQSGNDACVALAEAISGSEDNFAQMMNREAQRLGMKASSFRN
                     SAGMPDPQHYTTARDLAILAGALIRDFPDEYAKYYSLKEFRYNKITQPNRNRLLWLDP
                     TVDGVKTGHTDGAGYCLISSAKRGPRRLLSIVLGTKSDGVRTQESLKLLNFGFQFYDA
                     VQLYAKNQAVSDLKVWKGSEKTVKAGFTHDFILAVPKGSGPRVKAELVSQQPLIAPVT
                     QGQVVGTMKVSIDGSPIGDYPVVAIEGVPAAGIFGRAFDTMRLWFN"
     gene            3070..3951
                     /locus_tag="PLD53_01805"
     CDS             3070..3951
                     /locus_tag="PLD53_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HRC59669.1"
                     /translation="MTGKTAYLNGRYLPLAEAKISPLDRGFLFGDGAYEVVPVYSRRP
                     FRLDEHLRRLQTTLDGIRLPNPHNAEEWKAIVLHVIAEADFADQSVYIEVTRGADSKR
                     DQAFPIGVAPTIFVFTSPLITPTLEQRESGIAVISAVDIRWGRCDLKTVALLANVLMR
                     QLAVDAGCAETIMLRDGYLTEGAASNIFVVQDGVILAPPKDHRMLSGITYDVVLELAA
                     QHGMPHQVRPIAEAELRSADELWMTSSTKEVLAITTLDGQPVGKGTGAGKPGPILRRM
                     YDWYCAFHDEVSRRADA"
     gene            3944..4222
                     /locus_tag="PLD53_01810"
     CDS             3944..4222
                     /locus_tag="PLD53_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HRC59670.1"
                     /translation="MPDSAQSPETLLVFPCAFPVKIMGLTNDALAQSVLDVVLRHAPD
                     YDAACMEMRASSGGKYVSLTCTINATSRSQLDALYRELTGHPLVKVVL"
     gene            4219..4848
                     /gene="lipB"
                     /locus_tag="PLD53_01815"
     CDS             4219..4848
                     /gene="lipB"
                     /locus_tag="PLD53_01815"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRC59671.1"
                     /translation="MNTAPAVRWLGRVAYQPTWQAMVDFTAARNAETADELWVCEHPP
                     VYTLGQAGKPEHLLTDIGIPLIKIDRGGQITYHGPGQVVIYLLLDLTRRGLKVRELVR
                     RIEQAVIDLLAGQGVAATRLSGAPGVYIVQGDAVAKIGALGLRVRRGGCYHGVSLNVD
                     MDLSPFAAINPCGLAGMAVTQTRDLGIALSPLQVAESLVNHLNRQLERA"
     assembly_gap    4976..5075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5076..5645
                     /locus_tag="PLD53_01820"
     CDS             <5076..5645
                     /locus_tag="PLD53_01820"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRC59672.1"
                     /translation="ITSVDRDDLRDGGAGHFVNVIAAVREKSPATIIETLVPDFRGRL
                     EIAIDVLGRSLPDVLNHNMETVPRLYRQARPGADYAHSLAFMQQFKARYPQVPTKSGL
                     MVGLGETDDEIISVLRDLRANGVEMLTIGQYLAPSAHHLPVMRYVHPDTFAMYEHEAV
                     AMGFTGAACGPMVRSSYWADQQAHSAGVA"
     gene            complement(5706..6335)
                     /locus_tag="PLD53_01825"
     CDS             complement(5706..6335)
                     /locus_tag="PLD53_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HRC59673.1"
                     /translation="MRSLSTFVFLAVLLSACSSTPKTSYYTLSAAPLPSAAATSSGTS
                     VMVGPVSLPDSLDQPLLVVQSGNNQVSMSEYHRWAGSLKGDIGRVIAVNLAHDLGTAR
                     VWSYAQSIQTNADYQVLIDVQSFDAKLGDAVELDVLWTILPAASKATPGMTTSAKSTP
                     RPSADPGSNQKSGRTLVHEPVSGAGFESLVAAQSRALVRVSADIARAIR"
     gene            complement(6332..7996)
                     /locus_tag="PLD53_01830"
     CDS             complement(6332..7996)
                     /locus_tag="PLD53_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRC59674.1"
                     /translation="MTDSRGDPDLPNLPTAVAKHKRRWAPQFIWIIPILAVLVGLGLA
                     VKVVMERGPTITITFKTGDGLEAGKTHVKYKDVDVGVVKAVALSADHQAVIATVQLDK
                     DAGDFLLDDTRFWIVRPRITASGVTGLGTLLAGPFIAVDVGKATSKRKEFVALDVPPI
                     VTGDTPGREFILRASDLGSHEIGVPVYFRRLTVGEVVAYDLDKNGKGVSIRVFVHAPY
                     DKYVTSNTRFWNASGVDVSLSAAGIQVQTESLVSILIGGIAFEAPQSSDGTVIDIVNE
                     PTTIASSTPAEGPPADRAEADTVFPLFLTREAAMKRADAHVERFVINFKQSVRGLSVG
                     APVEFRGVDIGEVVRIDTEFDPKTFDFVQPVEILLYPDRLRARSQTGGASLPPPKTNA
                     QRIKRAQLFVAKGFRAQLRSGNLLTGQKYIGVDFFHDAPKVTLDTTRFPLVIPAIPGE
                     FDEIEKTIASVIKKMDRLMADTDKLVKRLDAETIPEFNQTLVESRRALKSAENVMASD
                     SPVLIDLRETLRELTRAAASIKALTDMLDQQPQSLIFGKPPEEQKP"
     gene            complement(7989..8669)
                     /locus_tag="PLD53_01835"
     CDS             complement(7989..8669)
                     /locus_tag="PLD53_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HRC59675.1"
                     /translation="MSATVSATAPMTGIDAGVVACHTCGMAHQASPEAHGLNCVRCGA
                     SLHFRKPDSLSRAWALLIAAYVLIIPANVLPVMETGSLFGTEDDTILSGVIYLWTSGS
                     EPLAIILFIASIAIPAAKLVSLTYLLVSVQRHSPFAPRERTRLYRILEAVGRWSMIDV
                     YVASMLTALVQFGSLMSIRAGSGAIAFGAVVVLTMFAAESFDPRLIWDTAGKDSAGKT
                     NNLENEND"
     gene            complement(8666..9274)
                     /locus_tag="PLD53_01840"
     CDS             complement(8666..9274)
                     /locus_tag="PLD53_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HRC59676.1"
                     /translation="MSSEALVVCHECDLLLREVPLEAGGVARCTRCGCNLYRNMPEGL
                     ERSLVFSLTAAALFVIANAFPIVSMNSQGLTSSTTLLGMVQVLYDDEMFSVALLVFIT
                     AFLMPALEIAAFIYLLLPLRLGRTVRGIPLVFRLLHTAQPWGMIEVFMLGILVTISKL
                     AAMASVVPGIALWSFVLLMIAIAAASAGFDSRSFWARVESLR"
     gene            9388..9684
                     /locus_tag="PLD53_01845"
     CDS             9388..9684
                     /locus_tag="PLD53_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59677.1"
                     /translation="MNWRRSVPSGASVAYRAGLVDSGITPHADIARRTGLIDAGVYRT
                     DPGSADAWPSDIACRTRLVDSGIDYPNSGSANARYSTHGARLIHTAISGARNRR"
     gene            complement(9843..10376)
                     /locus_tag="PLD53_01850"
     CDS             complement(9843..10376)
                     /locus_tag="PLD53_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRC59678.1"
                     /translation="MKTRRKFMAAVALAAAATPLGALLNPASAAENKVQLMFVQSAES
                     IKADAKTLRLVNVAPQTIYFSDRPERIAGHISMPDYLKEWTAASGPNNFSKDPPNATL
                     SVYEADKEANTLTVIEISKPVVEGRDLVYNYKLLEGKVPSSGGATALFIDWIGPGGGV
                     GVGFHGVGVGRRGVGWR"
     gene            complement(10446..10868)
                     /locus_tag="PLD53_01855"
     CDS             complement(10446..10868)
                     /locus_tag="PLD53_01855"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRC59679.1"
                     /translation="MKQTEMGKSLSRRKLLGGLALLVCTQPAWAIFGVWRRAARRTVF
                     VAGAATTAAVVTESAMASQQAAAASSAQAAANESAAAANQAAAAANRAEASAAQAANP
                     PKTPQQRLKELQSLYDQGLISASDYETAKKKILSQITQ"
     gene            complement(10897..11511)
                     /locus_tag="PLD53_01860"
     CDS             complement(10897..11511)
                     /locus_tag="PLD53_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HRC59680.1"
                     /translation="MKMSCFAGGLLATALCLANPACAKDTGQAAASAPSKETAVGMAL
                     ERAMMPGEGQKRLEAMIGTFDVAIRTWVDPTKPPIESSATSVSVWVLDKRYIQMMLAG
                     KLDGEAFSGIGYIGFDNVTKLYEATWMDTGSTGMTWYKGGFDASGKSATMKASVPDTL
                     TGKPAPLELRMNISADGGHVTELWGKGLGNKMFKMMELRYTRSK"
     gene            11864..12436
                     /locus_tag="PLD53_01865"
                     /pseudo
     CDS             11864..12436
                     /locus_tag="PLD53_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
     gene            12506..12829
                     /locus_tag="PLD53_01870"
     CDS             12506..12829
                     /locus_tag="PLD53_01870"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HRC59681.1"
                     /translation="MVARHALTQRSSRLVRFYEAGIIGLGNDGMVKMRDGSHLTLVQR
                     QIAEKLIDQENPDRNSLIYALAEAHGGKAVQPAARAAQVKRWKDQFHSGWWVEDANGN
                     WSKKP"
     gene            12922..13377
                     /locus_tag="PLD53_01875"
     CDS             12922..13377
                     /locus_tag="PLD53_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRC59682.1"
                     /translation="MKILRSIVLTILLGAVSGAAMASDVDVKAIWVRGTVAGQKATGA
                     FMEVTSESGATLVGAFSPVAGLTEIHEMRMEGGVMKMRAVTRLELPAGQTVKLAPGGY
                     HVMLMDLKQALKPGEFVPLSLTVEGKGKKTETIEVKGEVRDLTAMPMKH"
     gene            complement(13397..14344)
                     /locus_tag="PLD53_01880"
     CDS             complement(13397..14344)
                     /locus_tag="PLD53_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRC59683.1"
                     /translation="MNSADGRLRQIRVIAQCVTGLTLMVVLVSACMRLEGAGLGCADW
                     PECYAQVLSSEPLALHFGIVRILHRIGASSSLLLACFLVWRCLRPYPIQPAALQALLL
                     LMLMLLLAILGPWSSDPRLPLVGFLNIMGGLLLVNLSWRVVLSTDTTPESENAGRAGI
                     LLRLGVASLSLTVMLGAWMGASYAAVACSTLPDCNGVWWPSAKGWAALNPWIRLTAAP
                     LPGDAGGVALHLLHRYLALAAVLLLGMTCLRSLRNAATRVSALAVLALLFFEFGLGGA
                     SVSSGLNLWLVVSHGVCTAVLLAAVITLQRRQRSVATMQ"
     gene            complement(14344..15393)
                     /locus_tag="PLD53_01885"
     CDS             complement(14344..15393)
                     /locus_tag="PLD53_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRC59684.1"
                     /translation="MDSVARRQIALWLFICSAMVFAILVVGGVTRLTHSGLSIVEWKP
                     IVGVIPPLNQSEWDETFEKYKKTPEYQKVNHQMELDEFKGIFFWEYWHRVLGRLIGAV
                     FLLPFLYFWLRRKIDRPLVPKLLGIFVLGGLQGAMGWYMVKSGLVDDPRVSQFRLTAH
                     LSLAFLIFISMMWVALDLLTARQRSTASEALRKLQRTGIMLAILAFYTVVTGGFVAGI
                     RAGKAYNTFPLMNGHFLPPESFIIEPWYLNFFNNMALVQFDHRLGAWLLAFLVPWFFL
                     KVWAANVSNRARLAAAFLLVSLIIQISLGIATLLLAVPVALGTAHQGGSMVVFGALLW
                     LNHELRVVRGPAAIA"
     gene            15634..15783
                     /locus_tag="PLD53_01890"
     CDS             15634..15783
                     /locus_tag="PLD53_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59685.1"
                     /translation="MAENNDDDVPFMQKLLDNTFLLLFLGVASPGLLYILWGIIDIMN
                     VPLAK"
BASE COUNT         3380 a         4750 c         4632 g         3109 t
ORIGIN      
        1 gagcacgacg aggtgtggtt ggtagcgctc aagaaaggcg tcgagctggc gcgtatccgg
       61 cagcaggagc gtgtcatagc cgtgtcggcc aagataggtg gccagcaact ggcgcagttc
      121 agggtcatca tcgacgagca gaattttttt catcatcgca tcttgattcg gaacgatgcc
      181 cattctatgc cgccgctgca gggttgaaag gcgtctgaaa caatttgcaa tcaaccgccc
      241 cggcaatgaa atcctttgca acaggccaag gcagaaaatg gcatcgtcaa aacaggcgtt
      301 gcatcttcgc atcgcacttg acttacacgt ggttatccac ggcatctcaa cctgaaaaca
      361 gaggagcttc accatgaatg aaagatattc gaaaatgatc ccacgccgaa tcgcgggcac
      421 cctggccgcc gtagccgtgt cggcgacgat ggccaccgcg gccttcgcca tgccctgggg
      481 tggggactgc gacggcaaat ccggtgatcg aaccgagtgt cgttcagaac gaggcatgaa
      541 gggaatgtcc cgactgcacg acgaactcaa gctggacgcc aaacaggaag cgctgtggca
      601 agacgctgaa aaagccagca aggaaaacat ggccggcatg cgcgatcgct tccgccagca
      661 ccacgaagaa attcaggcac tgatcgacaa acccggtgcc gacctgcgcg cggtagccaa
      721 gcgcatggat gagttcaagg ccgaggggca gaaactccgt gaagccaacc gtgatcgctg
      781 gctgacggtg tatgactcac tcaacgccga gcagaaagag aaagctcgtg tgttcttcaa
      841 gagcaagctg gagcgcatgg cgcgccatgg ccaccgcggc cctggtcgga gttaacagcc
      901 gaatcgccga tggcgattgc cgtgaggggt gggaaatgac ccgcaggtca tcacctcccg
      961 cccctctatt cccgcatcga gtcattgagt tggcggcgat atgcatctgc tgatttgtac
     1021 gataatggta aacaattctt ctccgtgcag tccggaaggt tttcaccatg ccccgttcaa
     1081 tccgtcggat cgtcatttcg ggagttaccc gcggcctcgg ccgggccatg ctggatgagt
     1141 tcgtctcgct cggccacact gttgccggtt gtggtcgttc ggaagcggca atcgatgacc
     1201 tccgcttgaa gtttgcagcg ccgcaccgct ttgcgacggt ggacgttgcc gacgattttc
     1261 aggtggggca atgggcgcgt catgtgttga cgacaatggg gccgcccgac atcctgatca
     1321 ataacgccgc gctgacgaac aagccggcgc cgttatggga ggtatccgcg gcggaattcg
     1381 acgcactcat cgacgtgaac gtcaaaggag tggccaatat gttgcgtcat ttcgttccgg
     1441 ccatgatcgc cggggaacgt ggcgtgatcg tcaatttcag ctccggctgg ggtcgcagca
     1501 ccgcgcctga cgtagctcca tactgcgcca gcaagtgggc catcgaaggc ctgacgggtt
     1561 cgctggccca ggagttgccc gaagggatgg cggccgtcac gatgaatccg ggaatcatcg
     1621 ataccgacat gctgcggctg tcctttggtg aaaccgccgg cggatacgaa gcgccctccg
     1681 cctgggccga gcgcgcggtc ccgctcttgt tgaagctggg gccgaagaac aacggcaagg
     1741 cgctgaacat tccctagccg cctgccgtcg gcacatcgat ggcgaagcgc ggtcgttcaa
     1801 gcgtcgccgt gccgcgacgg aggcgatttc cgcgtttgct gttacaaaat cgcacaagga
     1861 atgccgggcg ggagcagggg tgccacttgc tataattgcg tcttttaccc aatacacggt
     1921 tccccatgct caagtttcga tcactgctta ttgcccttct cgtttctttc ctcgtcccgt
     1981 cgctggccct ggcccagcaa atgcccacgc cgccggctct ggccgccaaa tcctggctgc
     2041 tcctcgaact cgaatcgggg caggagctgg catcgcaggc gcccgacgag cgcatcgagc
     2101 cggcttccct gaccaagctg atgacggctt acctcacttt tgcggcactc aagcagggga
     2161 cgatcaagct cgaccagatg gttacggttt ccgagaacgc ctggaagtcg caaggctcgc
     2221 gcatgttcat ccaggtcgat acgcaggtgg aaatcgagga cctgatcaag ggaatgatcg
     2281 tgcagtccgg caacgatgcc tgcgttgcgc tggccgaagc catctccggc agcgaggaca
     2341 acttcgcgca gatgatgaac cgggaggcgc agcgccttgg catgaaggcg tcgagtttcc
     2401 gcaattcggc gggcatgccc gacccccagc actacaccac ggcgcgcgat ctggcgatcc
     2461 tggcgggagc gctgatccgg gacttcccgg acgagtacgc caagtactac tcgctgaagg
     2521 aattccgcta caacaagatt acccagccca atcgcaatcg cctgctgtgg cttgatccga
     2581 cggtcgatgg cgtgaaaacc gggcacacgg acggggcggg gtactgcctg atttcttcgg
     2641 ccaagcgcgg cccgcgccgt ttgctgtcga ttgttctggg tacgaaatca gacggcgttc
     2701 gcactcagga gtcgctcaag ttgctcaact tcggcttcca gttctatgac gccgtacaac
     2761 tctatgcgaa gaatcaggcc gtatccgatc tcaaggtctg gaagggttcg gaaaagaccg
     2821 tcaaggccgg cttcacccac gacttcattc tcgccgtgcc caaaggttcc gggccgcgcg
     2881 tgaaggccga actggtttcg cagcagccgc tgatcgcgcc ggtgacgcaa ggtcaggtgg
     2941 tcgggacgat gaaggtcagt atcgatggct caccgattgg cgattacccc gtggtggcca
     3001 tcgagggagt gccggccgcc ggtatattcg ggcgggcttt cgacaccatg cgtttgtggt
     3061 ttaactgacg tgacggggaa gaccgcctat ctgaacggcc ggtatctgcc gctcgcggag
     3121 gcgaagatct cgccgctcga tcgcggcttc ctgttcggag atggcgcgta cgaggtcgtc
     3181 ccggtctatt cgcgccgccc gtttcgcctt gacgaacacc tgcgccgcct gcaaacgacg
     3241 ctcgacggta ttcgcctgcc caacccgcac aatgccgagg agtggaaggc gatcgttttg
     3301 cacgtcattg cagaggccga tttcgcggat cagtcggtct atatcgaagt gacgcgcggc
     3361 gcggacagca agcgcgatca ggcttttccg attggtgtcg caccgacgat atttgtcttt
     3421 acctcaccgc tgattacgcc cacgcttgag cagcgcgaaa gcggcattgc cgtaatcagc
     3481 gccgtcgaca tccgctgggg gcgttgcgac ctcaagaccg tggccttgct ggccaacgtg
     3541 ctgatgcgtc aactggccgt cgacgccgga tgcgccgaga ccatcatgct gcgcgacggt
     3601 tatctgaccg aaggcgcggc gtccaatatc ttcgtggtgc aggatggcgt catcctggcg
     3661 ccgccgaagg atcaccgcat gctgtccggc atcacctacg acgtggtgct ggaactagct
     3721 gcgcagcacg gtatgccaca tcaggttcgt ccgatcgccg aggccgaatt gcgttctgct
     3781 gacgaattgt ggatgacgtc ttcgaccaag gaagtgctgg cgatcaccac gctcgacggc
     3841 cagcccgtcg gcaagggcac cggagcaggg aaaccgggcc cgatattgcg ccggatgtac
     3901 gactggtact gcgcgtttca cgatgaagtg agcaggcgtg ccgatgcctg attcggcgca
     3961 atcgccggaa accctgctcg tatttccctg cgctttcccg gtcaagatca tgggcctgac
     4021 caatgacgcg ctggcacagt cggtgctcga tgtggttctg cgtcatgccc ctgactacga
     4081 tgcggcttgc atggagatgc gtgcatcgag tggcggcaag tacgtcagcc tcacctgtac
     4141 catcaacgcg acctcacgat cccagctcga cgcgctctat cgtgaactta ccggccaccc
     4201 tctggtcaag gtggtgcttt gaatactgcg cctgctgtcc gctggctcgg gcgggtagcg
     4261 taccaaccga catggcaggc gatggtggat ttcacggccg cccggaacgc cgaaacggcg
     4321 gatgagctgt gggtttgcga gcacccgccg gtctatacgc tggggcaggc cggcaagccg
     4381 gagcacctgc tgaccgacat cggcattccg ctgatcaaga ttgaccgcgg cgggcagatc
     4441 acctaccacg gtcccggaca ggtggtgatc tatctgctgc tcgatctgac gcggcgcggc
     4501 ctcaaggtgc gtgagctggt caggcgtatc gagcaggcgg ttatcgatct gctggccggg
     4561 cagggtgttg cggcaactcg gctgagcggc gctcccggcg tgtatattgt tcaaggtgac
     4621 gcggtggcca agatcggcgc actggggctg cgcgtcagac ggggtggctg ctatcatggt
     4681 gtcagtctca atgtcgatat ggatctgtcg ccttttgccg cgatcaaccc ctgtggtctg
     4741 gccggcatgg cggtaactca gacccgtgat ctgggaatcg cgctgtcgcc gctgcaagtg
     4801 gctgaatcgc tggtcaatca cctgaacaga caactggagc gagcatgagc gagcagcaga
     4861 gtgaccagaa tgatgatcaa agcagtgagc gagtgagccc ggcgtccccc cggatcgctg
     4921 gcgtcaagga gcgtggcgaa ctgaaaacgg cgcgaattcc gatcaaggtt gtcgcnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatcac cagtgtcgac cgcgatgacc
     5101 tgcgtgacgg cggagccggg cacttcgtca acgtgatcgc cgcggtgcgc gaaaaatcac
     5161 cggcgacaat catcgaaacg ctggtgccgg acttccgcgg gcgcttggaa attgcgatcg
     5221 acgtgctcgg ccggtcgctg cccgacgtac tcaaccacaa catggagacc gtgccgcgcc
     5281 tctacaggca ggcgcgcccc ggtgccgatt atgcgcattc actggccttc atgcagcagt
     5341 tcaaggcgcg ctatccgcag gtgcccacca agtctggcct gatggtaggg ctgggcgaga
     5401 ccgacgatga aatcatcagt gtgctgcgcg acctgcgcgc caacggcgtc gaaatgctga
     5461 ccatcggcca atacctcgcg ccctctgccc atcatttgcc ggtgatgcgc tacgtccatc
     5521 cggatacctt tgcgatgtac gaacacgaag ctgtggccat ggggtttacg ggcgccgcgt
     5581 gcggtccgat ggtgcgttca agttactggg cggatcagca ggcacacagc gccggcgtcg
     5641 cctgagcggc ccgccgaggc ggctctcgaa aaacaagggt tgaacaggcc cggctagcga
     5701 tccgactagc gaatcgccct ggcaatgtcg gcgctcaccc gcacgagcgc ccggctttgc
     5761 gcggcgacca gcgactcgaa gccggccccg gaaaccggct catgcaccag agtacgtccg
     5821 ctcttctggt ttgaaccggg gtctgcgctg gggcgagggg tggattttgc actggtggtc
     5881 atgccgggtg ttgccttgct cgcagcaggc agaattgtcc ataacacgtc aagctcgacc
     5941 gcatcaccga gcttggcatc gaacgactgg acatcgatca gcacctgata atccgcattc
     6001 gtctgtatgc tttgtgcgta actccacacg cgtgccgtgc ccagatcgtg cgccagattg
     6061 accgcgatca cgcgcccgat gtcgcctttg agcgagcccg cccaacggtg atactcggac
     6121 atgctcacct ggttgtttcc gctctgcacc accagaagcg gctgatcgag cgaatccggc
     6181 aggcttaccg gtccgaccat cacactggtg ccgctgctag ttgccgctgc cgaggggagt
     6241 ggcgccgcac tcaaggtgta gtaagacgtt ttcggcgtac tgctgcacgc actcagcaga
     6301 accgccagaa atacaaaggt tgaaagtgat ctcatggctt ttgctcctct ggcggcttgc
     6361 cgaatatcag ggattgcggc tgctgatcga gcatatcggt caaggctttg atcgatgccg
     6421 cagctcgcgt cagttcgcgc agggtttcgc gcagatcgat cagtacgggc gaatcgctgg
     6481 ccattacgtt ttcggcgctc ttcaacgcac gcctcgattc aaccagggtc tggttgaatt
     6541 caggtatggt ttcggcatcg aggcgtttaa cgagtttgtc ggtgtcagcc atcaggcgat
     6601 ccattttctt gatcacgctg gcaatcgtct tttcgatttc gtcgaattcg cctggaatcg
     6661 ccgggatgac aagcgggaac cgggtagtat ccaaagtgac tttcggtgcg tcatggaaga
     6721 aatcgacgcc gatatatttc tgtcccgtca ataagttgcc gctccgcaat tgcgcgcgaa
     6781 aacccttcgc aacaaacagt tgggctcgct ttatgcgctg cgcgttcgtt ttgggtggcg
     6841 gcagcgaggc accgccggtc tgtgagcgcg cccgcaggcg atcggggtaa agaagaatct
     6901 cgaccggttg cacgaaatca aaggtttttg gatcaaactc ggtatcgatg cggacgacct
     6961 cgccgatatc gacgccacga aattcaacgg gcgcgccaac cgacaagccg cggacggatt
     7021 gcttgaaatt gatcacaaag cgttcgacgt gtgcgtccgc gcgtttcatc gccgcctcac
     7081 gcgtcaggaa caagggaaac acggtgtcgg cttccgcacg atcagcaggc ggcccttctg
     7141 caggcgttga tgaggcgatc gttgtcggct catttacgat gtcaataacc gtcccatccg
     7201 aagactgggg cgcctcaaaa gcgatgccgc caatcaggat tgacactaaa gattccgttt
     7261 gcacctgaat gccggcagca ctgagcgata catcaacacc gctggcgttc cagaagcggg
     7321 tattgctcgt cacatactta tcataaggcg catgcacaaa cacacggatc gacacccctt
     7381 tgccgttttt atccagatcg taagctacga cctcgccgac cgtcagccga cggaaataaa
     7441 cgggcacgcc gatctcatgc gatcccagat cggacgcgcg caagataaac tcgcgtccgg
     7501 gcgtatcacc ggtcacaatt ggcggtacgt ccaaggccac gaactctttt cgcttgcttg
     7561 tcgccttgcc aacatccact gcgataaagg gaccagccag caaagtcccc aggcctgtca
     7621 ccccacttgc tgtgatgcgt ggccgaacga tccagaaacg cgtgtcgtcg agcaggaaat
     7681 cgccagcatc cttgtccaac tgaaccgtgg caatcaccgc ttgatgatcg gcggacaagg
     7741 ccacggcttt gacgacaccc acatccacat ctttgtactt cacatgggtt tttcctgcct
     7801 cgaggccatc gcccgtcttg aaggtaatag tgatggttgg accccgctcc ataacgacct
     7861 tgaccgccaa gccaagtcct accagaacgg ccagaattgg aatgatccag atgaattgcg
     7921 gtgcccagcg ccgcttgtgt ttggccacgg ccgtgggcaa attcggcaga tcgggatcgc
     7981 cccgcgagtc agtcattctc attctccaga ttgttcgttt ttcctgccga gtcttttcct
     8041 gctgtatccc aaatcagccg aggatcgaaa ctctcggcgg caaacatcgt cagcaccacc
     8101 accgcaccga aggcgatggc gcccgacccg gcgcgaatcg acatcaggct accgaactgt
     8161 accagcgccg tgagcatgga cgcgacatag acatcgatca ttgaccagcg cccgacggct
     8221 tccagaatac ggtacagacg cgttcgctca cgcggcgcaa aaggcgaatg ccgttgaacg
     8281 gaaaccagca ggtacgtcaa ggaaaccagt ttggccgccg ggatcgcaat actggcaatg
     8341 aacaggatga tcgccaacgg ctccgaaccc gaggtccaca agtagatcac gccgctcagg
     8401 atcgtatcgt cttcggtgcc gaagagcgag ccggtttcca tcactggcaa cacgttggcc
     8461 ggaatgatca gcacataggc agcaatcagc agggcccagg cgcgcgacag gctgtccggt
     8521 ttgcgaaaat gcagacttgc gccacaacgc acgcaattca agccatgcgc ttccggcgat
     8581 gcctgatgcg ccatgccgca ggtatggcag gcgacgacgc ccgcgtcaat gccggtcatc
     8641 ggggccgtgg cggatacagt cgcactcatc gcaaggactc cacgcgcgcc cagaaactac
     8701 gcgaatcgaa accagcggaa gccgccgcga tggcaatcat caacagcaca aacgaccaca
     8761 gtgcaatgcc gggaaccacg gaagccattg cggccagctt gctgatcgtc accagaatgc
     8821 ccagcatgaa cacctcgatc atgccccagg gctgggccgt atgcagcagc ctgaatacca
     8881 gcggaatgcc gcgtaccgta cgccccaacc tgagcggcaa gagcaaatag atgaacgccg
     8941 caatttccag cgcaggcatc aggaaggcgg taatgaacac gagcagcgca acactgaaca
     9001 tctcgtcgtc gtatagcacc tgcaccatgc cgagcagggt cgtcgatgaa gtcagtccct
     9061 gcgaattcat gctcacgatc gggaaggcat tggcgatgac gaagagcgcc gccgcggtga
     9121 gcgagaaaac caggctgcgc tccaggcctt cgggcatgtt gcggtacaga ttgcagccgc
     9181 aacgggtgca acgggctaca ccgcctgcct cgagcggcac ttcgcgcagg agcagatcgc
     9241 attcgtggca gacaaccagt gcttctgatg acatcgaaat tacctgttca tgtcgcatgc
     9301 tcgagccgtg ggttcaagca agcaaaacct gtgctgcata tgcaagggca ctccgcttgg
     9361 cgcttgcccc atcgaagcgt tgagcggatg aattggcgtc gatcagtacc gtctggcgcg
     9421 tcggttgcct accgcgccgg gttggttgac tccgggatca cgccccacgc cgacattgcc
     9481 cgccgcaccg ggttgattga cgccggggtc taccgcaccg accccgggag cgccgacgcc
     9541 tggccttccg acattgcctg ccgcaccagg ctggttgatt ccgggatcga ctaccccaac
     9601 tccgggagcg ccaacgcccg ctactccacc cacggcgcca ggctgattca caccgcgatc
     9661 tctggcgctc gaaatcgcag gtaacgcgat aagcgtcgtc cccaaggcca aggccacccc
     9721 ccattttgtc gctgtacatg tcgttgctga tttcatcttg gcctccatcc attccggttg
     9781 ttgggtaaag cagaacaggc gatcaccgca cgcccttcaa acgatcaagt ctgacaaatc
     9841 gtttagcgcc agccgactcc gcgtctgccg acgccgacgc catggaaacc gacgcccaca
     9901 ccgccacccg gtccaatcca gtcgatgaac aaggccgttg cgccaccaga cgaaggcacc
     9961 ttgccttcga gcagtttgta gttatagacc agatcacgcc cttcaacgac aggcttgctg
    10021 atttcgatca ccgtaagcgt gtttgcttcc ttgtctgcct catacaccga caacgtggcg
    10081 ttgggcggat ccttgctgaa attgttgggg ccggatgccg ccgtccactc cttcagatag
    10141 tccggcatcg aaatgtgccc ggcgatgcgc tccggtcggt cggagaaata aatcgtctgc
    10201 ggcgcaacat tgaccaagcg cagcgttttg gcgtccgctt tgatgctttc ggcgctttgt
    10261 acgaacatca gctggacctt gttttccgca gcactcgccg ggttgaggag cgcacccagc
    10321 ggggtagcgg ctgcagccag cgcgacggct gccatgaatt tgcgtcgtgt cttcatctgt
    10381 cttcctttct agccaagaat atgaactgga gacggcaatc agcgcgtcgg tgcagctcgc
    10441 acgccttact gcgttatttg actgaggatc ttctttttgg ccgtttcgta gtcgctcgcg
    10501 ctgatcagtc cctggtcgta cagcgattgc agttccttga gcctctgctg cggcgttttg
    10561 ggtgggttcg ccgcctgtgc cgcactggct tccgctcgat tcgcggctgc cgcagcctgg
    10621 ttggcggcgg cggcagattc gttcgctgca gcctgcgccg atgacgctgc cgcggcttgc
    10681 tgagaggcca tcgcgctctc ggttactacc gcagcggtgg tggctgcccc ggcaacgaac
    10741 actgtccgcc ttgcggcacg ccgccagacg ccgaagatcg cccaagctgg ctgcgtgcac
    10801 accagtagcg ccagcccgcc cagcagtttc cgccgtgaaa ggctcttgcc catttccgtt
    10861 tgcttcattt ctactctcct ttaccggtcg gcctgactac ttgcttcgcg tataccgcag
    10921 ttccatcatc ttgaacatct tgttgcccag gcctttgccc cacagctcgg tcacatgacc
    10981 tccatcagcg gagatattca tccgcaactc gagaggtgcc ggcttgccgg tcaaggtgtc
    11041 gggaaccgac gccttcatcg tcgccgactt gccggaggcg tcgaaccccc ccttgtacca
    11101 ggtcatgccg gtactgccgg tgtccatcca ggttgcctca tataacttgg tgacgttgtc
    11161 gaagccgatg tagccaatgc cgctgaacgc ctcgccgtca agcttgcccg ccagcatcat
    11221 ctggatgtag cgcttgtcca atacccacac gctgacgctg gttgccgacg attcgatcgg
    11281 gggtttggtt gggtcgaccc aggtgcggat ggcgacgtcg aaggtgccga tcatggcctc
    11341 aagccgtttt tggccttcgc ccggcatcat cgccctctca agcgccatgc ccacagccgt
    11401 ctccttggac ggagcgcttg ccgcagcctg gccggtgtct ttggcgcagg cggggttagc
    11461 cagacagagg gcggtagcca gcagcccccc cgcaaaacag gacattttca ttttccaagt
    11521 ctccttatca aacccgttgg ttacccgcaa gaacaagcat catgcactga cgtacttcgc
    11581 atgcagagcg taccatcccg acacccatac gcattgacaa attctgacat gaaattgacc
    11641 cctcgcgaca atcgccccgt gaaaaagaca agtcgcggat aagccattcg agtctaccca
    11701 gcccggtgca acactctcgc caatctcgga aagcggatgc ctttataatc cacatcaaag
    11761 cgctgtaaag ggcgccacgg aaaatgagcg cgtgagtcag actgttccgt gattcgtttt
    11821 gtctttgttg ccttcgcctg cccggaacgg gccccttgaa accatgaaaa aactatttgc
    11881 cctgatcctg agcttcgtcg tgctgctgac cggttgtacg gatccgccca aattcaaatc
    11941 aaccgacatc accggctcgg actggggcaa ggacttcgcg ctcaccgacc acaacggaac
    12001 acctcgccaa ctggcagact tccgcggcaa agtgctagtc gtattcttcg gctataccca
    12061 gtgcccggat gtttgtccaa ccacgctcgc ttcgatgcgg gatgtgctcg gtcttctcgg
    12121 cgaagatgcc aagcgcgtac aggttttgtt cattaccctg gatccggcgc gtgacagctc
    12181 ggagttgctg gcgcattacg tacccgcgtt tcatcccagt tttcttgggt tgcgtggtga
    12241 cgatgatgcc acggctgcag tggccaaaga gttcaaggtg ttttacagca agcagaaggg
    12301 cagcacgccg gataattaca gcgtcgatca ctcgactggc agctatgtct tcgaccccga
    12361 cggcaggttg aggctgctgt tgcgccacgg cgaggcgccc gaaatcgtcg ccggcgacct
    12421 gaagctttta ctggcgaaca gcaaaacacc cgccgtcgtc accggtccgg ctgagatcaa
    12481 tctggaaatc ggtacaccgc cggtgatggt cgcacgtcac gcgttgactc agcgttcatc
    12541 ccggctggtc cggttttatg aggcgggcat tatcggactg ggcaatgacg gaatggtcaa
    12601 aatgcgcgac ggaagtcatt tgacgctggt gcagcgccag attgcggaga agctgatcga
    12661 ccaggaaaac ccggatcgca actcgctcat ttatgcgctg gccgaagctc atggcggcaa
    12721 agcagttcaa cccgctgccc gagcggccca ggtaaaacgc tggaaggatc agttccactc
    12781 agggtggtgg gttgaggatg ccaacgggaa ctggagcaag aagccctgat ctgcgcaagt
    12841 cccccgttga ttggcaacat gcttggcgca acggcgatcc tgacatggcg acacgcatct
    12901 atttctcaaa aggaatatca catgaaaatt cttcgatcga tagtgctgac gatcctgctt
    12961 ggcgccgttt ccggtgcggc aatggcgtcc gatgttgatg tcaaagccat ctgggttcgc
    13021 ggcacggttg ccggccagaa ggctacgggt gctttcatgg aagttacgag cgaatcaggg
    13081 gcaacactcg ttggtgcgtt cagtccggtc gcaggactga cggaaataca cgaaatgaga
    13141 atggagggcg gcgtcatgaa aatgcgtgcc gtcacgcgcc tggagctgcc tgccggccag
    13201 acggtcaaac ttgctcccgg gggctatcac gtcatgttga tggacctgaa acaagcgctc
    13261 aagccgggag aattcgtacc cttgtctctg actgtcgagg gcaagggcaa gaaaacggaa
    13321 accatcgaag tcaagggcga agttcgtgac ttgaccgcga tgcccatgaa gcactgatcg
    13381 ggacgcgaga aagcggctac tgcatcgtgg ccacgctacg ttgccgtcgc tgcagcgtga
    13441 taacggcggc cagcagcact gccgtacata cgccgtgact gaccaccagc cacagattga
    13501 gcccgctgct gaccgacgcc ccacccagac cgaattcaaa aaatagcagg gccagtactg
    13561 ccagtgctga gaccctcgta gccgcatttc tgagcgaccg tagacaggtc atgccaagga
    13621 ggagaaccgc ggcgagtgca agatatcgat gcagcagatg aagtgccacc ccaccagcgt
    13681 cacccggcag aggggcggcg gtcagcctga tccatggatt caatgctgcc cagccttttg
    13741 ctgacggcca ccacacacca ttgcagtcgg gcagagtgga acaggccact gcagcgtaac
    13801 tggcgcccat ccacgctccg agcatgacgg taagggaaag cgacgcgacg cccagacgca
    13861 gcaggatgcc tgcccgcccg gcattctccg attcgggagt ggtgtcggtt gataagacca
    13921 cacgccagga aagattcacc aggaggagtc ctcccatgat gttgagaaag ccgaccagcg
    13981 gcagccgggg gtcggaactc cagggaccca ggatcgccag caacagcatc agcatcagga
    14041 gcagcaaggc ctgcagggca gcgggctgaa tcgggtacgg gcgcaggcag cgccagacaa
    14101 gaaagcaggc cagcaacaga gaagacgagg cgccaatgcg gtgcagtatt ctgacgattc
    14161 cgaagtggag cgccagcggt tcgctcgaca gcacttgcgc gtaacattcc ggccagtcgg
    14221 cacagcccag gccggcaccc tcaaggcgca tacaggcact caccagcact accatcagag
    14281 tcaacccggt cacgcattgc gcgataaccc tgatttgccg caagcgcccg tcagcggaat
    14341 tcatcaggcg atagcggccg gtccgcgcac gacccgaagt tcgtggttaa gccagagcag
    14401 ggcgccaaaa acgaccattg agccgccctg gtgcgcggtg cccagggcga cgggaactgc
    14461 cagcaggagc gtggcaatgc cgagcgatat ctgaatgatc agggacacga gcaaaaatgc
    14521 ggcagccagc ctcgctctgt ttgatacgtt ggcggcccag accttcagaa aaaaccatgg
    14581 caccaggaaa gccagcaacc acgcacccag acgatgatca aactgcacta gagccatgtt
    14641 gttgaagaag ttgaggtacc acggctcgat gatgaaactc tccggcggaa gaaaatgacc
    14701 gttcatcagc ggaaaagtgt tgtaggcctt gccggcgcgg atgcccgcaa caaagccacc
    14761 ggtaacgacc gtatagaagg ccaggatggc cagcatgatt ccggtgcgtt gcagttttcg
    14821 caatgcctcg ctcgccgtac ttcgttgccg cgccgtcagc aggtcaagcg ccacccacat
    14881 catcgagatg aaaatcagga aagccagcga cagatgggca gtcaggcgaa actggcttac
    14941 ccgaggatca tcgaccaatc cgctcttgac catgtaccag cccatcgcgc cttgcaagcc
    15001 gccaagaacg aaaatgccca gcagcttggg aaccaggggc cgatcgattt ttcggcgcaa
    15061 ccagaagtag aggaaaggca aaagaaagac agccccgatc agccttccca gcacgcgatg
    15121 ccagtactcc caaaagaaaa tgcctttgaa ctcgtcaagt tccatctggt gattgacttt
    15181 ctgatactcg ggcgttttct tgtatttctc gaaggtttca tcccattcgc tctgattcag
    15241 cggcggaatg acgccgacga tgggcttcca ctcgacgatc gacagcccgg aatgggtgag
    15301 ccgcgtaacc ccgccaacca caagaatggc aaacaccatg gcgctacaga taaacaacca
    15361 gagggcaatc tgtcgccggg cgacgctgtc catagttcaa ctccagagaa aaatcggtaa
    15421 atgcaggatt ataggatcat tcgccagcgc gttgtcccac acgctgctga ttactaatgg
    15481 tttttccgga tattgacgtg gatcaaatcg ccagatgcca aagcaaagta ctctttcccg
    15541 gatagtctgt ggtctcggag caagtaacag acttgctgtt gacaaaaaac caaccgtgcg
    15601 ccgaaggcgc caatcgttaa agagggtaga aacatggccg aaaacaacga tgacgatgtc
    15661 ccgttcatgc agaagcttct ggataacact ttcctgctcc tgtttctcgg tgtggcctca
    15721 ccagggctcc tctacatctt gtggggcatc atcgacatca tgaacgtccc cttagccaag
    15781 taagtcgttc cgttttctct gggagaatca aatgagtgca attctgccgc cgtcggatcg
    15841 gctctggtgg aaacaaccca tcgacaaggt cgagtgggca tggattggta tcgcgttcgt
    15901 gtggggcatg atcatgttcg tgatgatggt ctattggcat atctacggta agcagaacct
    15961 gtcgaacgag g
//
LOCUS       DAORPM010000019        15110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_779_length_15110_cov_8.116240, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000019 DAORPM010000000
VERSION     DAORPM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 15110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15110
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_779_length_15110_cov_8.116240"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /gene="htpX"
                     /locus_tag="PLD53_01895"
     CDS             complement(<1..819)
                     /gene="htpX"
                     /locus_tag="PLD53_01895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRC59686.1"
                     /translation="MKRILLFLGTNLAIVLVLSVTMRLLGVEPYLNANGLNLGSLLIF
                     AAVMGFGGSFISLAISKWSVKKSMGVQVIETPSNSTEFWLVETIRKYATEAGIEMPEV
                     GIYQAPEVNAFATGMSKNSSLIAVSTGLLQQMSRAEVEAVLGHEVAHAANGDMVTLAL
                     IQGVVNTFVMFLSRVIGHLVDKVVFKTERGHGPAFFITMIIAELVLGILASIIVMWFS
                     RQREFRADRGGARLAGRQNMIAALQRLNALHPQPLPDRMAAFGIAGGGGSGFKRL"
     gene            complement(1012..3735)
                     /locus_tag="PLD53_01900"
     CDS             complement(1012..3735)
                     /locus_tag="PLD53_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC59687.1"
                     /translation="MNAVAHKNPVHRRNDWHHLPVDQVADDLKTVLDDGLSDEAVSSL
                     RAQHGENRITPQPGRGPLLRFALQFSQPLVVVLVIAGVVTAFLGEWVDSGVIFGVTLI
                     NAVIGFIQEGRAEDALAALARSVTSEVTVLRGGAKRRLPSTALVPGDVVLLAAGDKVP
                     ADLRLFRAKELQAIESALTGESTAVSKHHKALSPDTLLAERGNMAYAGTVMVRGQGAG
                     VVVATGDATETGRISRLIAEAPDLTTPLTSKMAVFSNWLLIAIGLLAVLTFAIGLWRG
                     ESAFEMFMAAVALAVGAIPEGLPAAMTITLAIGVSRMAKRRAIIRKLPAVETLGSTTV
                     ICSDKTGTLTENQMTVREIFAGGVSYPVSGSGYAPTGKIADEPDLDSPDSCALRECLL
                     GGGLCNDAGLRKSGRHWEITGDPTEGALLVALRKGGLDEATLQKLFPRLDEIPFDSAR
                     QYMATLHEIEGVRIAYFKGAIEQLLPRSTSMLDAAGVPVPIDRERIEKIAGEMAVEGL
                     RVLAVGRLASLSGGSLDHKQIERDDCELQFVGLFGMIDPPRPKAISSVKACHAAGIVV
                     KMITGDHAVTALSIASQIGIVKAGAEAENTVLTGRDLAALDDDALRGVAQAVHVFARV
                     EPEQKLRLVRALQADGEVVAMTGDGVNDAPALKQANIGIAMGLAGTEVAKDAADMVLT
                     DDDFATIEAAVEEGRGVYDNLVKFITWTLPTNFGEGLVIMAAIVAGATLPITALQILW
                     INMTTAVFLGLTLAFEPIEKGVMRRVPRPPGTPILDHALIARIVLVGVLLLGGSFGLF
                     LLELARGHTLAEARTVAVNVFVVVETFYLFNCRSLSRSALKIGLFSNPAIWYGVATMA
                     VLQLSLTYVPLMNQLFQTAPIDAHAWMEIFAVGLSALLVVGVEKHLLHRRR"
     gene            complement(3874..4170)
                     /locus_tag="PLD53_01905"
     CDS             complement(3874..4170)
                     /locus_tag="PLD53_01905"
                     /inference="COORDINATES: protein motif:HMM:NF023171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HRC59688.1"
                     /translation="MDFLDILGLLLLVATGWIWFDSLKAREAAVSASKAACNSEDKLL
                     LDDTVAIQRIGLGRDDEGVLRIRRLYGFEYSDTGNDRCAGRIVMLGSQVLVINL"
     gene            complement(4225..6111)
                     /gene="recQ"
                     /locus_tag="PLD53_01910"
     CDS             complement(4225..6111)
                     /gene="recQ"
                     /locus_tag="PLD53_01910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRC59689.1"
                     /translation="MTHSADSSAVAILHELFGYKDFRGAQAEIVAHVLSGGDALVLMP
                     TGGGKSLCYQLPAIMRHRAGHGVAVVVSPLIALMHDQVGALEVAGVHAAFINSTLTLE
                     ETQRIEREMLSGRLVLLYVAPERLTTPRMLAQLDSLRERGLLSLFAIDEAHCVSQWGH
                     DFREDYQALNVLHERYPGVPRVALTATADDLTRADVIERLALSEARVFISSFDRPNIR
                     YAVVEKDDARRQLLHFIQEEHQGDAGIVYCQSRRKVDETAVWLDRQGISALPYHAGLA
                     AEVRRRHQDRFLRDEGLVMVATIAFGMGIDKPDVRFVAHLDLPKNIEGYYQETGRAGR
                     DGLPADAWMAYGLADVVNQRRMIDESPAAEAFKRNQRGKLDALLSLAEAHDCRRVRLL
                     AYFGEASVACGAHQNACDNCLNPPATWDATEAARKALSGVYRFLQQRGQRFGAGHLID
                     VLRGKTTEKVAQFGHESLTTFGIGSDVSEAQWRVVLRQLIALGYLQTEGDYNTLELTP
                     AAREVLRGEVQITLRQPGVASKAARVAKRGKGTSAQRTRGKSPPLPLDEAGLARFEAL
                     KAWRGELARERKLPAYVIFHDATLAEMAHTAPGSLEAIENISGVGAKKLEAYGRDILR
                     VLGG"
     gene            complement(6180..7478)
                     /locus_tag="PLD53_01915"
     CDS             complement(6180..7478)
                     /locus_tag="PLD53_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRC59690.1"
                     /translation="MARLLGPLSTLRWRTRAKLWFAALLAGLMVVAFAELADMALALF
                     GRLTTGRPWMPFVLAPTIGMLTVWLTRRLCPGAAGSGIPQVIAATWLAAKGKPVNTLL
                     SLRIAFGKIILGALALVGGFSAGREGPSVQVAASVMHSIHNIFPSSRALRAQDLILAG
                     GAAGVAAAFNTPLAGIVFAIEELGRRLETRTSGVLVSTIIISGLVAVALHGNYNYFGQ
                     LKVINQDRTIVLPIIVTGLVCGSLGGLFSRLMLWPQKRVDLKLWRWRAEHPIYFAGAC
                     GLVIAIIGLVGGGLSYGSGYMITSQVVSGELELPWHAPITRFLATIVSYYSGIPGGIF
                     APALAVGASLGSNLAQLLGIPADTIPIIALCMAGFLAAVTQAPITSAIIVMEMIDGHE
                     MVISLMAVTLIAKAVSARLSPELYQQLALGFRATKDPAGS"
     gene            8017..8493
                     /locus_tag="PLD53_01920"
     CDS             8017..8493
                     /locus_tag="PLD53_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRC59691.1"
                     /translation="MLHAVSAILALFSFWLVLSGFFTAFLISAGAASSLAVFAFSRRM
                     GVVDREGHPVHLSWRALTYWPWLIKEIVKSSWDVSKIILNPRLPISPTLVRIKPTQRT
                     PEGLVTHANSITLTPGTISVEVGPDEFLVHAVTREAAEGVASGDMDRRATRFEGSA"
     gene            8495..8776
                     /locus_tag="PLD53_01925"
     CDS             8495..8776
                     /locus_tag="PLD53_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRC59692.1"
                     /translation="MFAAAALALLVALGLTLTRAVLGPTVFDRAQAANTIGTIAVLLL
                     AVIGFLNGRPEFLDLAIVYGLLNVIGTIAVLKYFTYGDLGEPGEEEEEQ"
     gene            8773..9102
                     /gene="mnhG"
                     /locus_tag="PLD53_01930"
     CDS             8773..9102
                     /gene="mnhG"
                     /locus_tag="PLD53_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRC59693.1"
                     /translation="MSLLLEWVSWACLLAGGFFCVVGALGLLRMPDFYTRMHAASVTD
                     TLGAGLILFGLMLQAGLTLVTAKLLIIVLLIAFTSPTATHALAKAALTRGVQPQLDED
                     GEPPSKP"
     gene            9111..9638
                     /locus_tag="PLD53_01935"
     CDS             9111..9638
                     /locus_tag="PLD53_01935"
                     /inference="COORDINATES: protein motif:HMM:NF009159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRC59694.1"
                     /translation="MLLAMMAVTVVALTRSRNLFAVVVLGGIYSFLMATVLVALDAVD
                     VAMTEAAVGAGISTVLLLGALHLCKSEEAKPLHRPWLPLMLALATGATLVYGTLGLPA
                     FSDPAAPIHAHVVPRYLVDGPRETGVPNVVTAVLASYRGFDTLGETTVVFTAGIGVIA
                     LLRRRIGRKDDEDIK"
     gene            9635..10063
                     /locus_tag="PLD53_01940"
     CDS             9635..10063
                     /locus_tag="PLD53_01940"
                     /inference="COORDINATES: protein motif:HMM:NF009162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HRC59695.1"
                     /translation="MKSYLVLRVIAKMLIPFMLLFALYVQFHGDFGPGGGFQAGVIAA
                     AAVIFYSLIYGLPKARKVVPGWLVETMLAAGVLIYAGVGIAGIFLDGNFLDYFVLSAD
                     SVAGQHRGIFWVEVGVATTVSGVMLKIFYMFAGRGRQESE"
     gene            10060..10428
                     /locus_tag="PLD53_01945"
     CDS             10060..10428
                     /locus_tag="PLD53_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HRC59696.1"
                     /translation="MNTGSHFAYWITVFLLMAGLYILIARGNLIKKLVGLGIFQTSVY
                     LLYISPGKLIGGTAPIFATAFTVYSNPLPHVLILTAIVVGVATLALGLALVVRIREAY
                     GTIEEDEILALDDEREREKE"
     gene            10425..11897
                     /locus_tag="PLD53_01950"
     CDS             10425..11897
                     /locus_tag="PLD53_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HRC59697.1"
                     /translation="MIAAHLPALQVVLPLISAPLMVLVRRGGIAYGLMLTVAWISLLI
                     SLLLAQQVAEFGPISYHIGNWPPPWGIEYRVDALSSFVLVLVSLAGAVVVPYSRRSVM
                     SEVPQALHYLFYSMFALCLTGLLGITITGDAFNIFVFLEISSLSTYVLIALGRDRRAL
                     TAAYQYLIMGTIGATFIVIGVGLLYLMTGSLNLVDIAQRLPAVAEKRPVLAALAFLTV
                     GISLKLALFPLHLWLPNAYTYAPSSISAFLAATATKVSVYVLIRFYYSVFGDSLVFRD
                     LQILLVLSIAAMITASLAAIYQNNLKRLFAYSSVAQIGYITLGLSLDSVGGLSAAIVH
                     LFNHALAKGALFMLIGGIMLRTGDVTLSHAAGLARRMPVTALGIVIAGLTLIGVPGTA
                     GFVSKWALITASLEAGRWWLVPLIVASSLLAVAYVWRFVEAAYLREPPPESQEKNEAP
                     WSMLVPAWLLVALSIYFGVDTRFTLGGATRAAQMLLGVGG"
     gene            11894..13363
                     /locus_tag="PLD53_01955"
     CDS             11894..13363
                     /locus_tag="PLD53_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRC59698.1"
                     /translation="MIGTQTAIGAALIVPLAGALAIALLGRRPNLRETATLSTATLLF
                     FCVISLLAPVSAGERPELTLFEMFPGLSIAFRVEPLGMLFALVASGLWIVNSLYSIGY
                     MRGNDEQKQTRFYVCFAGALAAAMGIAFAANLLTLFLFYETLTLITYPLVTHKGSEDA
                     RRGGRTYLGLLIGTSMLFLLPAIAITWHVAGTLDFVSGGILDGKLSGFALGGLLALFM
                     FGIGKAALMPFHRWLPAAMVAPTPVSALLHAVAVVKAGVFSVLKVVVYVFGTGTLASA
                     TNTDWLVAIAGFTIVASSVVALHADNLKRRLAYSTISQLSYIVLAAAILAPLSIIGAA
                     LHIATHAVGKITLFFAAGAIYTAAHKTEVSQLDGIGRRMPWTMAAFTVGALSLIGIPP
                     AAGFISKWFIMSGAADAGQYGALAVLIISTLLNAAYFLPIVYAAFFRPLSAEDAHHPH
                     GEAPRLMVVALMLTTTATLSLFLFSAPLLELVEQLVVPR"
     gene            13392..13640
                     /locus_tag="PLD53_01960"
     CDS             13392..13640
                     /locus_tag="PLD53_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59699.1"
                     /translation="MSGPKEYWLDSSENVTKLFRGLCILGLALLAIDLVLHKHEAFAF
                     AAWFGFYCLFGFFACVVLVLVAKQLRRILMRDEDYYER"
     gene            13675..>15110
                     /locus_tag="PLD53_01965"
     CDS             13675..>15110
                     /locus_tag="PLD53_01965"
                     /inference="COORDINATES: protein motif:HMM:NF009310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HRC59700.1"
                     /translation="MAVPFLRGPLRTAYMLLLPIIAFAYLLALPYGEFGQVELLGQTL
                     VLMRVDRLSLLFGYIFVLASLLNVIYAIHLKDTMQHVAGLVYAGSSIGAVFAGDLMTL
                     FMFWELTAVSSVLLIWAQRTDTTFRTGLRYVVVQLLSGMFLLAGGLLHWRATGSLAFG
                     HIGLSGLAGTLILIAFGIKCAFPLLHNWLQDAYPDATPTGAVILSAFTTKLGIYALAR
                     AYPGTEMLIWIGTLMAAFPIFYAVIENDLRRVLAYSMNNQLGFMVVGIGIGSELAING
                     AVAHAFADILFKGLLFMSMGAVLLRTGTVKGSELGGLYKSMPWTTGFCIVGAASISAF
                     PLFSGFVTKSMIMSAAADEGLLLTWLVLLFASAGVFHHAGIKIPYFAFFSHDSGIRCE
                     EAPRNMLIAMGVAAALCIGIGVFPDALYSILPFPVTYQPYTSSHVVAQLQLLVFSALA
                     FAVLMRTGIYPPELRSVNLDSDWFYRVFL"
BASE COUNT         2760 a         4945 c         4384 g         3021 t
ORIGIN      
        1 caggcgcttg aatccgctgc cgccgccacc ggcaatgccg aacgcggcca tccggtcagg
       61 caagggttgc ggatgtagcg cattcaggcg ctgcaatgcg gcgatcatgt tttgccgacc
      121 agccaggcgc gcacctccgc gatcggcgcg gaattcgcgc tgccgcgaaa accacatgac
      181 gatgattgac gccagaatgc ccagcaccag ctcggcgatg atcatggtga tgaaaaacgc
      241 ggggccatgg ccgcgctcgg tcttgaacac gaccttgtcc accagatggc caatgacgcg
      301 agacaggaac atgacaaagg tgttgacgac gccctggatc agcgccagcg tcaccatgtc
      361 gccgttggcg gcatgtgcca cctcgtgccc gagcaccgcc tcgacctccg cgcgcgacat
      421 ctgctgaagc aagccggttg agacggcaat cagcgaactg ttcttgctca tgccggtagc
      481 aaaagcgttg acctcgggtg cctggtagat cccgacctcc ggcatctcga taccggcttc
      541 cgtcgcatac ttgcgaatgg tctcgaccag ccagaattcg gtcgaattcg acggtgtttc
      601 gatgacctgt acacccatcg attttttgac cgaccacttg gatatggcca gcgagatgaa
      661 agagccgcca aagcccatga cagccgcaaa gatcaggagt gaaccgaggt tcaatccgtt
      721 ggcgttgagg tagggctcga cacccagcag gcgcatggtg acggacagta ccagcacgat
      781 ggccagattg gtgccgagaa acagcagaat tcgtttcatc gcgattccta agaagtattc
      841 gtttacaagg tgtaccgtgc gcacgatctc ccgcacgacg tggcaccata tggaggccag
      901 atgcacgttc ttcaagtaca ttatccggca cgcgcgcaag ctgagctgga acacgacgtc
      961 gattgttaca ttgcggaaat cgtcgatgtc ggatcgattt caacttcaga attaccggcg
     1021 tcgatgcagg aggtgttttt ccacgccgac gaccagcaat gcagaaagac ccaccgcgaa
     1081 aatctccatc cacgcatggg cgtcaatcgg tgcggtttga aacagttggt tcatcaacgg
     1141 cacataggtc agggacaact gcagaacagc catcgtcgcc acgccgtacc agatcgccgg
     1201 attgctgaag aggccaattt tcagcgccga gcgactcaag gaacggcagt tgaacagata
     1261 gaatgtctcg accacgacga aaacgttgac cgccaccgtg cgggcctcgg ccagggtgtg
     1321 cccgcgcgcc agctccagca ggaagaggcc gaaggaacca ccgagcaaca acacgccgac
     1381 cagcacgata cgggcgatca atgcgtgatc aaggatcggc gtgcccggcg gtcgcggcac
     1441 gcgacgcatg acgcctttct cgatcggctc gaaggcaagg gtcagaccga gaaacaccgc
     1501 cgtcgtcatg ttgatccaca gaatctgcag cgccgtgatc ggtagcgtgg cgccggcaac
     1561 gatggccgcc atgatgacca ggccttcgcc gaaattggtt ggcagcgtcc aggtgatgaa
     1621 cttgacgaga ttgtcgtaga ccccgcgccc ctcctcgaca gccgcctcaa tcgtggcgaa
     1681 atcgtcgtcg gtcagcacca tgtccgccgc atccttggcc acctctgtgc cggccagacc
     1741 catggcgatg ccgatattgg cctgtttgag tgccggcgcg tcattgacgc cgtcgccggt
     1801 catcgccaca acctcgccat cggcctgcag ggcacgcacc agtcgcagct tctgttcggg
     1861 ttcgaccctg gcgaaaacat gcacggcttg cgccacgccg cgcagggcat cgtcgtcgag
     1921 cgcggccaga tcacgcccgg tcagtacggt gttttcagcc tctgcacccg ccttgacgat
     1981 accgatctgg ctggcgatcg aaagcgccgt cacggcatgg tcgccggtaa tcatcttgac
     2041 cacgattccg gctgcatgac aggcttttac tgacgaaatc gcctttggcc gcggcggatc
     2101 gatcatgccg aacagcccga cgaactgaag ttcgcagtca tcccgttcaa tctgtttgtg
     2161 gtccaaggaa ccgcctgaaa gggacgccag gcgcccgacc gcaagcacac gcaagccctc
     2221 gacagccatc tcgccagcaa ttttctcgat ccgctcgcga tcgataggca ctggcacgcc
     2281 tgccgcatcg agcatgctcg tcgagcgcgg cagcaactgc tcgatggcgc ccttgaaata
     2341 cgcaatacgc accccttcaa tttcgtgcag ggttgccatg tactggcgtg ccgaatcgaa
     2401 gggtatttca tcgagtcggg gaaacagttt ctgcagcgta gcttcgtcaa gcccgccctt
     2461 gcgcagcgca acgagcaacg caccttcggt cggatcaccg gtaatttccc aatgccgccc
     2521 agatttgcgc aagccggcat cgttgcacag cccgccgccg agcaggcatt cgcgcagtgc
     2581 acaggaatcc ggcgaatcca ggtcgggctc gtcggcaatc ttgccggtcg gcgcatagcc
     2641 actgccgctc accgggtagc tcacgccacc ggcaaaaatt tcgcgtaccg tcatctggtt
     2701 ctcggtcagc gtgccggtct tgtccgagca gatcaccgtc gtgctgccca gcgtttcgac
     2761 cgccggcaac ttgcggatga tcgcgcggcg tttggccatg cgtgaaacgc cgatcgccag
     2821 cgtgatcgtc atcgccgccg gcaatccctc gggaatcgcc cccaccgcca gcgccacggc
     2881 ggccatgaac atttcgaaag ccgactcgcc gcgccacagt ccgatcgcga aggtcagcac
     2941 cgcgagcagg ccgatggcga tcagcagcca gttgctgaaa accgccatct tgctcgtgag
     3001 cggcgtggtg agatcgggcg cttcggcgat caggcgcgag atgcgtcctg tctcggtcgc
     3061 gtcgccggtt gccaccacga cgccggcgcc ctgaccgcga accatcaccg tgccggcata
     3121 agccatgttg ccgcgctcgg cgagcagggt atccggcgaa agtgccttgt ggtgcttgct
     3181 cactgccgtc gactcaccgg tcagcgccga ctctatcgct tgcagttctt tcgcgcgaaa
     3241 caggcgcaga tcggccggca ccttgtcacc cgccgccagc agcacgacat cgccaggaac
     3301 cagcgccgtc gacggcaaac gtcgtttggc accgccgcgt agcaccgtca cttcgctcgt
     3361 caccgaacgc gccagagccg ccagggcatc ttcagcacgg ccttcctgga tgaagccgat
     3421 caccgcattg atcagcgtga ctccaaaaat cacccccgaa tcaacccatt cgccaagaaa
     3481 agccgttaca acgccggcga tcaccagcac aaccaccagc ggctggctga actgtagcgc
     3541 aaagcgaagc aagggccccc ggccgggttg tggcgtgatg cggttttcgc cgtgctgtgc
     3601 gcgcagggaa ctgactgctt cgtccgacag accatcgtcc agcaccgtct tcagatcgtc
     3661 agccacctga tcgaccggaa gatgatgcca gtcattcctt cgatggaccg ggttcttatg
     3721 agccactgcg ttcatttcgt tgcgccagat tgaacaatat cgaattttgg gccgccgaac
     3781 ggagaatcag ggccatcaac gcttgccgat cgtcgatttc gctcgcacct gatgttccca
     3841 tacttccgca agcgcataaa taccgcagcc tgattacagg ttgatcacca gcacctgact
     3901 tcccagcatg acaatgcgtc ctgcacaccg atcattaccc gtgtcactgt actcaaaacc
     3961 atagaggcga cgaatgcgta agacgccctc gtcatcccga cccagaccaa tgcgctgaat
     4021 ggcgacggtg tcgtcgagca gcagcttgtc ctcggaattg caggccgcct tcgaagccga
     4081 caccgccgcc tcgcgggctt tgaggctatc gaaccatatc cagccagtcg caaccagcag
     4141 cagaagcccg agaatatcca gaaaatccat gcggacagct taaccgaaat tgtcgctcgt
     4201 ccctgcgctc gggcgattgc tcaatcaccc gcccaacacg cgcaggatgt cgcgcccgta
     4261 ggcctcaagc ttcttcgcgc ccacaccgct gatgttttcg atggcctcca gtgaaccagg
     4321 cgccgtgtgc gccatttcag ccagagtcgc atcgtggaag atgacgtagg ccggtagttt
     4381 gcgttcgcgc gctagctcgc cgcgccacgc cttcagcgct tcgaaacgcg ccaggccggc
     4441 ttcgtcgagt ggtaacggcg gcgacttgcc cctggtgcgc tgagcactgg tgccctttcc
     4501 acgcttcgcg acgcgcgccg cctttgacgc aacgccgggc tggcgcagcg ttatctgcac
     4561 ctcgccgcgc aaaacctcgc gcgctgcagg cgtgagctcc aaagtgttgt agtcgccttc
     4621 ggtttgcagg tagcccagcg cgatcagttg acgcaacacg acacgccatt gcgcttcgct
     4681 cacgtcggag ccgatgccaa aagtggtgag actctcatgc ccgaactgcg caaccttctc
     4741 cgtagtcttg ccgcgcaaca catcgatcag atgcccggcg ccgaagcgct ggccgcgctg
     4801 ctggaggaaa cgataaacgc cggaaagtgc tttgcgcgca gcctcggtgg cgtcccaggt
     4861 cgctggcgga ttcagacagt tgtcgcaggc gttctggtgc gcaccgcatg cgacactcgc
     4921 ctcgccgaaa taggcgagca gccggacgcg ccggcaatcg tgcgcttcgg ccagcgacag
     4981 caacgcgtcg agcttgccgc gctggttgcg cttgaacgcc tcggcggccg gactctcgtc
     5041 gatcatgcgc cgctggttga ccacgtccgc caagccgtaa gccatccagg catcggcagg
     5101 caagccatcg cggcccgccc gaccggtctc ctggtagtag ccctcgatgt tcttcggcaa
     5161 atccaggtgc gcgacgaagc gcacgtctgg cttgtcaatc cccatcccga aagcgatggt
     5221 ggcgaccatg acgaggccct catcgcgcaa gaagcggtcc tgatgacgcc ggcgcacctc
     5281 ggcggcgagc ccggcatgat agggcaacgc actgataccc tgacgatcca gccacactgc
     5341 cgtctcatcc accttccggc gtgactggca gtaaacgatg cctgcgtctc cctggtgttc
     5401 ctcctggatg aagtgcaaca actgccggcg tgcatcgtcc ttttcgacca cggcgtaacg
     5461 gatgtttggt cggtcgaaac tgctgatgaa aacgcgggct tcgctcaatg caagccgctc
     5521 aatgacgtcg gcccgcgtca gatcgtcggc cgttgcagtg agcgcgacgc gcggcacacc
     5581 ggggtagcgc tcgtgtagca cgttaagcgc ctgataatcc tcacgaaaat cgtggcccca
     5641 ctggctcacg caatgcgcct catcgatggc aaaaaggctg agcaggccgc gctcgcgcag
     5701 cgaatcgagc tgcgcgagca tgcgcggcgt ggtcaagcgt tcgggtgcca cataaagcag
     5761 caccagacgg ccgctcaaca tttcgcgctc aatccgctgc gtttcctcca gcgtcagagt
     5821 cgaattgatg aacgccgcgt gcacaccggc cacttcgaga gcaccaacct gatcgtgcat
     5881 gagcgcgatc agcgggctca caacgaccgc cacgccgtga ccggcgcggt gccgcatgat
     5941 cgccggcagc tggtaacata ggctcttgcc gccaccggtg ggcatcagca ccagcgcgtc
     6001 accaccgctg agcacatgcg cgacaatttc tgcttgcgcg ccgcgaaagt ccttatagcc
     6061 aaacaattcg tgcaggatcg cgacagcgga cgagtcggcc gaatgggtca caaggcaggc
     6121 cgacgtcaag aaaacatgcg taaacgagcg agtccgactg cagtctggcg gagttgcatt
     6181 caggatcctg cagggtcttt cgtggcccga aacccgaggg ccaactgctg gtatagctcc
     6241 ggactcagcc gagcactcac ggccttggcg atcagagtga ccgccatcag gctgatcacc
     6301 atttcgtgac cgtcaatcat ctccatgacg ataattgccg aagttatggg cgcctgcgtt
     6361 accgcagcca ggaatccagc catgcacagg gcgattatcg gaatcgtatc cgcgggaatt
     6421 cccaacaact gggccagatt ggagcctaga gaggctccaa cggccaaggc tggagcgaaa
     6481 attccgccag gaatgcccga gtagtaactc actatggtgg ccagaaaacg agtgatcggc
     6541 gcgtgccagg gaagctccaa ttcaccggac acaacctgtg aagtgatcat gtagccgctg
     6601 ccatacgaga ggccgccgcc gaccagaccg ataatcgcta tcaccaaacc acaggcgccg
     6661 gcaaaatata tcgggtgctc ggcccgccaa cgccacagct tgagatccac tcgcttttgc
     6721 ggccataaca tcaagcggct gaacagccca cccaatgagc cgcagacaag tccggtaaca
     6781 atgatcggca gaacgatggt tcgatcctgg ttgatcactt tgagctggcc gaaataattg
     6841 taattgccgt gcagggcgac ggccaccaga ccggaaatga tgatggtgct gaccaacacg
     6901 ccgctggtgc gtgtttcaag tctgcgcccc agctcctcaa tggcgaagac aataccagcc
     6961 aaaggtgtat tgaacgctgc agccacgccc gccgcacctc cagccaggat gagatcctga
     7021 gctcgcaacg cgcgggaact cggaaatata ttgtggatgg aatgcatgac cgaggcagcg
     7081 acctgaaccg aaggtccctc acgccctgcc gagaaccctc ccaccagagc cagcgcgcca
     7141 aggattatct tgccgaaggc aatccgcaac gacaacaagg tattgacggg ttttcctttg
     7201 gcagcaagcc aggttgcggc gatcacttgc gggattccgc tccccgcggc tcccggacag
     7261 agccgtcgcg tcagccatac ggtcagcatg ccgatggtcg gcgccagcac gaaaggcatc
     7321 caggggcggc ccgttgtgag cctgccgaaa agtgcaagcg ccatatccgc cagttcggcg
     7381 aacgcaacga ccatcaggcc cgccagcaat gctgcaaacc acagctttgc ccgggtacgc
     7441 caacgcaaag tgctgagtgg tccaaggagt cgagccaatt gctattcttt caacaaagcg
     7501 ttcaatccga acgacattat atcggcagcg gacagaatgc tctcacccat tccttgcgcc
     7561 aaaccgcctg cggagctgga accatgattc ttgacggttc ggcacatttt gcatggctca
     7621 actttctgta agcttacact gctcttgctc attgatcgac ctccaactct tgcggccgtc
     7681 tgcactctaa ttgacgctca tgcggatcat gccgggcaac ggcaagaacg tcatacgcga
     7741 cgccagtgtt ccgcctgtgc cgcaccggct tgtactcttg gctgaagctc aacggaaatc
     7801 agagaatcca tttgcgactg gcaaagggat agcgccgaag ttgctgcaca attgaagagt
     7861 ccgcggtcaa cctttgttac gcttacagtc aactttcatt aagtttatgt tcaatcatta
     7921 tttatcgggc cgccgcgttc gtatataatt tgcaactcgt cggccgccat ggtacgtcgg
     7981 ccctatccga tcctttcgtt gcccagggct ttctggatgc ttcatgcggt aagcgcgatt
     8041 ctggcgctgt tttctttctg gctggtattg tccggcttct ttaccgcgtt tctcatcagc
     8101 gcgggcgccg cctcgtcgct ggcggtcttt gccttttcgc ggcgcatggg cgtcgtcgac
     8161 cgcgagggac accccgtcca tctaagctgg cgcgccctca cctactggcc ctggctgatc
     8221 aaggaaatcg tcaagtcgag ctgggacgtc tcgaagatca ttctcaatcc acgtcttccg
     8281 atcagtccga cgctggtgcg catcaagccg acgcagcgca cgcctgaagg gctcgtcacg
     8341 cacgccaatt ccatcacgct cacgccgggc acgatttccg tcgaggtcgg ccccgacgaa
     8401 ttcctggtcc acgccgtcac ccgcgaggcc gccgagggcg tggcgagcgg cgacatggat
     8461 cgccgcgcca cccgcttcga aggcagcgcc tgacatgttc gccgcggccg cccttgccct
     8521 gctggtggcc ctaggcctca ccctgacgcg tgccgtactc ggaccgaccg tgttcgaccg
     8581 cgcacaggca gcgaacacca tcggcacgat tgccgtactc cttctggcgg tgatcggctt
     8641 cctcaacggc cgccccgaat tcctcgacct ggccattgtt tacgggctgc tcaacgtcat
     8701 cggcaccatc gccgtcctca agtatttcac gtacggcgac ctgggcgaac caggcgaaga
     8761 ggaggaagaa cagtgagcct gttgcttgaa tgggtaagct gggcctgcct gctggctggc
     8821 ggcttctttt gcgtggttgg cgcgcttggc ctcttgcgca tgccggattt ctacacacgc
     8881 atgcacgccg ccagcgtcac cgacacgctg ggcgccgggt tgatcctgtt cggcctcatg
     8941 ttgcaggccg gcttgacgct ggtgacggcc aagctgctga tcatcgttct gctgatcgcc
     9001 ttcaccagcc cgaccgccac ccacgcattg gccaaggcgg cgctgacgcg cggggtgcag
     9061 ccgcaactgg acgaggacgg ggagccgcca tcgaaaccct gatcatcaac gtgctgctgg
     9121 caatgatggc ggtcaccgtg gtcgcgctga cgcgcagccg gaatttgttc gcggtggtgg
     9181 tgctcggggg catctacagc ttccttatgg cgactgtgct ggtggcgctg gacgcggtcg
     9241 atgtggcgat gaccgaggcc gccgtcggcg ccggcatttc gacggttctg ctgctgggcg
     9301 ccctgcatct ttgcaagagc gaggaggcca agccgctgca ccgcccctgg ctgcccctca
     9361 tgctggccct ggcgaccggc gccacactgg tctacggcac gctcggcctg ccggcattct
     9421 cggatccggc ggctcccatc cacgcacacg tcgtaccccg ctacctcgtg gatggtcctc
     9481 gtgaaaccgg cgtgcccaat gtggtgaccg ccgttctggc cagctatcgc ggtttcgaca
     9541 cgctgggcga aacgaccgtg gtgttcaccg ccggcatcgg tgtcatcgcc ctgctgcggc
     9601 ggcgcatcgg ccgcaaggat gacgaggata tcaagtgaag agctatctcg tcctgcgcgt
     9661 cattgctaag atgctgattc ccttcatgct gctgttcgcg ctctacgtcc agttccatgg
     9721 cgacttcggg cccggtggcg gcttccaggc cggcgtcatt gctgctgccg ccgtcatttt
     9781 ctattcgctg atctacggtc tgcccaaggc gcgcaaggtc gtgccgggct ggctggtcga
     9841 aacgatgctc gctgccggcg tgctgatcta cgccggcgtc ggcatcgccg gcatcttcct
     9901 ggatggcaac ttcctcgact acttcgtgct gtccgccgac tcggtggcag gccagcaccg
     9961 tggcatcttc tgggtcgaag tcggcgtggc gaccacggtt tccggcgtga tgctgaaaat
    10021 cttctacatg tttgccgggc gcggcaggca agagtcggaa tgaacaccgg cagccacttc
    10081 gcctactgga tcaccgtctt cctgctgatg gcgggtcttt acatcctcat cgcgcgcggc
    10141 aacctgatca agaaactggt cggcctggga attttccaga cctctgtgta cctgctctac
    10201 atttcgcccg gcaagctcat cggcggcact gccccgatct tcgccacagc cttcacggtg
    10261 tactccaacc cgctgccgca cgtgctaatc ctcaccgcca tcgtcgtcgg cgtggcgaca
    10321 ctggcgctcg gactggcgct ggtggtgcgc atccgcgaag cctatggcac cattgaggaa
    10381 gacgagattc ttgcgctcga cgacgaacgg gagcgcgaaa aggaatgatc gccgcgcatc
    10441 tgcccgccct gcaggtcgtc ctgccgctga tttccgcacc gctgatggtg ctggtacgtc
    10501 ggggtggcat cgcctacggc ctgatgctga ccgtagcctg gatatcgttg ctgatctccc
    10561 tcctgctggc gcaacaggtc gccgaattcg gcccgatctc ctaccatatc ggcaactggc
    10621 caccgccctg gggaattgaa taccgcgtcg acgcgctgtc atccttcgtg ctcgtgctgg
    10681 tttcccttgc cggagcggtg gtggtgccct acagtcgccg cagcgtgatg tccgaggtgc
    10741 cgcaggcgct gcactacctc ttctactcca tgttcgccct ctgcctgacc ggcctgctcg
    10801 gaatcacgat caccggcgac gccttcaaca ttttcgtctt tcttgagatt tcctcgctgt
    10861 cgacctatgt gctgatcgca ctgggacgcg accgccgcgc cctgaccgcg gcctaccagt
    10921 acctgatcat gggcacaatc ggcgccactt tcatcgtcat cggcgttggc ctgctctacc
    10981 tgatgaccgg ctcgctcaac ctcgtcgaca tcgcccagcg actgcccgcg gtcgccgaaa
    11041 aacgaccggt gcttgcggcg ctggccttcc tcaccgtcgg catctcgctc aagctggcgt
    11101 tgttcccgct gcacctgtgg ctgcccaacg cctacaccta cgcgccgtcg tcgatttccg
    11161 cctttctggc ggccacggca accaaggtgt cggtgtacgt gctaatccgc ttctactatt
    11221 cggtattcgg cgacagcctg gtattcaggg atctgcagat tctgctggta ttgtcgatcg
    11281 cggccatgat tacggcctcg ctcgctgcca tctaccagaa caatctgaag cgcctgttcg
    11341 cctactccag cgtcgcacag atcggctata tcaccctggg actgtcgctg gattccgtcg
    11401 gcggcctctc cgccgccatc gttcatctgt tcaatcacgc gctggccaag ggcgcgctgt
    11461 tcatgctgat cggcggcatc atgctacgca ccggtgacgt aacgctgagt cacgccgccg
    11521 gacttgcgcg ccgcatgccg gtgacagctc tcggcatcgt catcgccggc ctgacgttga
    11581 tcggcgtgcc cggcaccgca ggcttcgtca gcaaatgggc gctcatcacg gcatcgctcg
    11641 aagcaggcag gtggtggctg gtaccgctca tcgtcgccag ttcgctgctg gctgtcgcgt
    11701 acgtctggcg cttcgtcgaa gccgcctacc tgcgcgaacc gccgcccgaa agccaggaga
    11761 agaacgaggc gccgtggtcg atgctggtgc cggcctggct gctggtagcc ctgtccatct
    11821 atttcggcgt cgacacccgc tttacccttg gcggtgcaac gcgcgccgcg caaatgctgc
    11881 tcggggtcgg cggatgatcg gtacgcaaac cgccataggc gccgccctca tcgtgccgct
    11941 ggccggagcg ctggccatcg ccctgctcgg ccgccggcca aacctgcgcg agacggcgac
    12001 gctgtccacg gccactctcc ttttcttctg cgtgatttcc ctgctggcgc cggtcagcgc
    12061 cggcgagcgg ccggagctga ctttgttcga gatgtttccc ggactctcga tcgccttccg
    12121 cgtcgaaccg ctgggcatgc tcttcgcgct ggtggcgagc ggcctgtgga tcgtcaactc
    12181 gctgtattcg attggctaca tgcgtggcaa cgacgagcaa aagcagacac gcttctacgt
    12241 ctgcttcgcc ggcgcgctgg ccgccgcaat gggcatcgct ttcgccgcaa atctgctgac
    12301 gctgttcctt ttctacgaga cgctgaccct gattacgtat ccgctggtaa cgcacaaggg
    12361 aagcgaggac gcgaggcgcg gcggacgcac ctatctcggc ctgctgatcg gcacctcgat
    12421 gctgttcctc ctgcccgcca tcgccatcac ctggcatgtc gccggcacgc ttgatttcgt
    12481 ttccggaggc atcctcgacg gcaagttgtc cggctttgcg ctcggcggac tgctggcact
    12541 cttcatgttc ggcatcggca aggcggcgct gatgcccttc caccgctggc tgccggcggc
    12601 gatggtggcg ccgacgcccg tttccgctct cctgcacgcc gtggcggtgg tcaaggccgg
    12661 tgtcttcagc gtgctgaagg tcgtggtcta cgtcttcggc accgggacgc tggccagcgc
    12721 aacgaacacc gactggctgg tggcgatagc cggcttcacc atcgttgctt cctcggtggt
    12781 agcgcttcac gccgacaatc tgaagcgccg gctggcctac tcgacgatca gccagctttc
    12841 ctacatcgtt ctcgccgcag caatcctggc ccccttgtcg atcatcggcg cggcgctgca
    12901 catcgccacg catgcggtgg gcaaaatcac gctgttcttt gccgccggcg cgatctacac
    12961 cgccgcccac aagaccgagg tcagccaact cgacggtatc ggccggcgca tgccatggac
    13021 catggccgcg ttcaccgtcg gagcgctgtc gctgatcggc atcccgcccg ccgcgggctt
    13081 catcagcaag tggttcatca tgtccggcgc ggcagacgcc ggccaatacg gcgcgctggc
    13141 ggtgctgatc atcagcaccc tgctcaacgc ggcctatttc ctgcccattg tttacgccgc
    13201 cttcttccgg ccgctgtcgg ccgaagatgc gcaccacccg catggcgagg cgccgagact
    13261 gatggtcgtc gcgctgatgc tgaccaccac ggccaccctg tcactgttcc ttttctcggc
    13321 cccgctgctg gaactggtcg agcagttggt ggtaccccgc taagcaatgc ccaactttat
    13381 ggatggcgac catgtcagga cctaaagaat attggctgga cagcagcgag aacgtgacca
    13441 agctgtttcg cggcctgtgc attctcggac tcgccctgct ggcgatcgac ctggtgttgc
    13501 acaagcacga ggcattcgct ttcgccgcgt ggttcggctt ctactgcctg ttcggttttt
    13561 tcgcctgcgt ggtgctggta ctggtagcca agcagctgcg ccgcatcctg atgcgcgacg
    13621 aggactacta tgagcggtga ctttcctgtc gcgctgatct ttgttctggg cgccgtggcg
    13681 gtgcccttcc tgcggggtcc gctgcgtacc gcctacatgt tgctgctgcc gataatcgct
    13741 ttcgcgtatc tgcttgcgct gccgtacggc gagttcggcc aggtcgaact gctcggtcag
    13801 acactggtgc tgatgcgcgt cgaccgtctg agtctgctct tcggctacat tttcgtgctc
    13861 gcctcgctgc tcaacgtgat ctacgcgatc catctcaagg acacgatgca gcatgtcgcc
    13921 ggacttgttt acgccggtag ctcgatcggc gcggtgttcg cgggcgatct gatgacgctg
    13981 ttcatgtttt gggaactcac ggccgtttcg tcggtgctgc tgatctgggc gcagcgcacc
    14041 gataccactt tccgcaccgg tttacgctat gtcgtggtgc agctactttc gggaatgttc
    14101 ctgctcgccg gcgggctcct gcactggcgg gcaaccgggt cgctggcgtt cggccacatc
    14161 ggtctttccg gcctcgccgg aacactgatc ctgatcgctt tcggtatcaa gtgtgccttc
    14221 ccgctgctgc ataactggct gcaggacgcc tacccggatg cgacccccac cggcgccgtg
    14281 atcctctcgg cgttcaccac caagctgggt atctacgcgc tggcgcgcgc ctacccaggc
    14341 accgagatgc taatctggat cggcaccctg atggcagcct tcccgatttt ctatgcggtc
    14401 atcgaaaacg acctgcgtcg ggtactggcc tacagcatga acaaccagtt gggcttcatg
    14461 gtggtcggca tcggcatcgg cagcgagctg gcgatcaacg gtgcagtggc gcatgccttc
    14521 gccgacatcc tgttcaaagg cctgttgttc atgtcgatgg gcgcggtgct gttgcgtacc
    14581 ggcacggtca agggcagcga actgggcgga ctatacaaat cgatgccctg gacgaccggc
    14641 ttctgcatcg tcggcgcggc ttcgatctcg gcgttcccgc tgttcagcgg tttcgtcacc
    14701 aaatcgatga tcatgtccgc cgcggccgac gagggtctgc tgctgacttg gctggtgctg
    14761 ctgttcgcct ctgccggcgt attccaccac gcaggaatca agattccgta ttttgccttc
    14821 ttttcgcatg actcgggaat acgctgcgag gaggcgccgc gcaacatgct gatcgcgatg
    14881 ggcgtcgctg ccgcgctgtg catcggcatc ggcgtcttcc cggacgcgct gtactcgatc
    14941 ctgcctttcc cggtcaccta ccagccctat acctcgtcgc acgtcgtcgc ccagctgcag
    15001 ctgctggtgt tctcggcgct cgcctttgcc gtgctcatgc gcaccggtat ctacccaccc
    15061 gaactgcgct cggtcaacct cgacagcgac tggttttacc gcgtctttct
//
LOCUS       DAORPM010000020        15047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_787_length_15047_cov_9.557764, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000020 DAORPM010000000
VERSION     DAORPM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_787_length_15047_cov_9.557764"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..566
                     /locus_tag="PLD53_01970"
     CDS             93..566
                     /locus_tag="PLD53_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRC59701.1"
                     /translation="MASLPVSFALASETVKIGYVNTQRIFRDAPTAVKAAKKLESEFS
                     KRDQDLQRMAKQIQSLQESLEKNALTMVETERRAKEKDLNELTREFQRKQREFREDLN
                     LRQNEENAAIIEKANKAIKQLAESEKYDLIVQDVVWVSPKLDVTDKIIKALSEGK"
     gene            573..1610
                     /gene="lpxD"
                     /locus_tag="PLD53_01975"
     CDS             573..1610
                     /gene="lpxD"
                     /locus_tag="PLD53_01975"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRC59702.1"
                     /translation="MPAQEGLRLDEIVSRLGGSLEGDASVIVSQVGTLASAGPGQIAF
                     LVNPKYRQQLQTTLAAAVIVPPQFAGDTALPRIIHHNAYAYYARVVALLNPSASRPAG
                     VHPSAVVHSLVPSSASIGENVVIGQDVAIGENVTLYPGCIIGDGVSIGDDSLFYPNVV
                     ICHHCVIGQRVLIQAGAVIGSDGFGFASEGEHWVKIPQIGRVVIGDDVEIGANTSIDR
                     GALDDTMIGHGVKLDNQIQIAHNVVIGDHVAMAGCVGIAGSTKIGRRCTIGGAGMITG
                     HLELTDDVHISAGTLVSKSLRKPGQYTSIFPLEVHEEWLRNAAQIKRLAKLAKRVSDL
                     EKKLEEMEKTS"
     gene            1616..2053
                     /gene="fabZ"
                     /locus_tag="PLD53_01980"
     CDS             1616..2053
                     /gene="fabZ"
                     /locus_tag="PLD53_01980"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRC59703.1"
                     /translation="MDIKEILDHLPHRYPFLLVDRVISFEPGKAIHAYKNITINEPYF
                     VGHFPHFPVMPGVLIMEALAQAAGILSFKTMGIKPDDNSVFYFVGIDNARFKKPVTAG
                     DQLHLHVEILRQMRGIWKYKAEARVDGEIVAEAELMCAKRDVA"
     gene            2082..2852
                     /gene="lpxA"
                     /locus_tag="PLD53_01985"
     CDS             2082..2852
                     /gene="lpxA"
                     /locus_tag="PLD53_01985"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRC59704.1"
                     /translation="MIHPSAIIHPGARLGSGVSVGPYSIIGENVEIGDNTWIGPHVVL
                     AGHTRIGCDNRIFQFCSLGEIPQDKKYAGEPTCLEIGDRNTIREFCTFNLGTALDAGV
                     TRLGDDNWIMAYVHIAHDCQVGNRTTFANNAQLAGHVHIDDWAILGGYTGVHQFCRVG
                     AHTMTAVGTVVLQDIPPFVMAAGNTASPFGINVEGLKRRGFSPDALKALKRAYRTLYK
                     SGFMLDEARAKLEEEVRSYPEIQPLLDFLAISRRGIIR"
     gene            2849..4033
                     /gene="lpxB"
                     /locus_tag="PLD53_01990"
     CDS             2849..4033
                     /gene="lpxB"
                     /locus_tag="PLD53_01990"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRC59705.1"
                     /translation="MTSEVVRIAIVAGEASGDLLASHLIQTLKAKLPGAVFYGIGGPK
                     MAGQGFDVWYPSEKLAVLGYAEALKHYFEISSIRSKLKRRLLADPPDIFIGVDAPDFN
                     LALEKCMKQHGIPSVHYVSPSIWAWRGQRIHKIGAAVSRVLALFPFEPVLYEKQGIPV
                     SYVGHPLADMLPLEDGRKSARELLGLSSQRPVFALLPGSRQSELNNLAEIFIRTACEI
                     HRKMPNALFLVPLATRETRLLFETALYRCDAREVPISLLFGHAHEAMMASDIVLVASG
                     TATLEAALLKRPMVIAYKMAPFSYRLMRRMGYLPYIGLPNILAGGFVVPEFIQDDATP
                     ENLAQALLNLYADKGICARIGEVFREIHLQLRQNNAEKAATAILDCLPVAVRNHASAA
                     AD"
     gene            4011..4598
                     /gene="rnhB"
                     /locus_tag="PLD53_01995"
     CDS             4011..4598
                     /gene="rnhB"
                     /locus_tag="PLD53_01995"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRC59706.1"
                     /translation="MRLLQPTEGLVCGVDEAGRGPLAGPVVAAAVILDPACPIDGLDD
                     SKKLTAKRREALAVKIRAKALSWAIAEASVEEIDRINILQASLLAMRRAVEALGIPPS
                     NALIDGNRCPQLACPAQAIIGGDGKVASIAAASILAKTFRDAGMLELHAIYPQYGFDQ
                     HKGYPTPAHLRTLREHGVSAVHRRSYAPVAQLILL"
     gene            4595..5374
                     /locus_tag="PLD53_02000"
     CDS             4595..5374
                     /locus_tag="PLD53_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRC59707.1"
                     /translation="MKHIASRDNPNFKALKKLSQSGRERRKRGRAVLDGMHLIESYIL
                     HFGQPEEVVVSESGSLRSEISRYLTSGRSGNVVSILSDALFDELAMVETPSGILAIVL
                     QPCPAHGPDQGIDTVLLDGIQDPGNLGSILRSAAAAGFRQILLSAECAQAWSPKTLRS
                     AMGAHFQLDIHESCDLVGFLAGYRGQVVLTALNGAVDLYSLALKEPLAWVFGSEGQGV
                     RPEVAATAQLQVRIPMPGATESLNVAAAAAVCLFETVRQRR"
     gene            complement(5377..6315)
                     /locus_tag="PLD53_02005"
     CDS             complement(5377..6315)
                     /locus_tag="PLD53_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRC59708.1"
                     /translation="MPGIFINYRRDDQAGFAGRLADVLENAFGVENVFRDVEDIHPGE
                     DFVVAIQKQLSSVSVMLVMIGPAWLTVNNNGVRRLNETDDFVRREIQAALESGKPVLP
                     VLVGGALMPEKADLPNEISALALRQAFVLSDASWDSDVARLVEFIRPLLPAQRRSQLR
                     RVVAWGLATVIVLSMLLIPNLKETPRNPSPLPTSPSPEQTDSRLTGHWTARVRYGWGA
                     EHDEIFELRLENGEMHGTASFLSLARIIEQGQLRGNQLSFVTHSQEVMNGSALREQTH
                     RYRGQLKSGELHLVLETTGGLSTHSPVEFTARRTLE"
     gene            complement(6319..7896)
                     /locus_tag="PLD53_02010"
     CDS             complement(6319..7896)
                     /locus_tag="PLD53_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59709.1"
                     /translation="MIPSGAVFFILISAVLLAAALAWVVAALYQRGMLALMRGGVPPA
                     TAMTGGNQSTLNLAAHHEPVTAAETNKADLGTNRHASVRLMLVLSGISLLIALTQSYL
                     ALFFIYQEGELSLNRVLLLGAIYAWPMVLAWGLARRWSWLRVLGGVILYMLVMLVLVM
                     ARSTEAQNLASASGWLGSQVAIPMTIIFLIGASGRIRAVAPYLLAPSVLLTGSSVLTL
                     QVMAQSAHNPAPWVIGLVGTLGAYPTLLLFALGPWLILAWPVFSFGKWLANAYRSKRF
                     SDLIYLIATYWFVILFASALPAMESVGWLAFVQVPALLWIPLVLFCLRGHLKPRLSPP
                     TLLVLRVFQRDAQVERLFDQVIERWRLSGNTVLIAGTDLLSRTLDPDDLFVYLNGTLA
                     ERFIANPAQIGERIAGFDWQPDPDGRYRINECYCFDSTWQSALHALVARADVVLMDLR
                     GFRMENRGCRYELGVLAGDSRLRRIVVLYDRDTARTEAESDIDAAAAGRFEWLSADQM
                     DSAMTDRVLSALFSDKV"
     gene            complement(7958..8920)
                     /locus_tag="PLD53_02015"
     CDS             complement(7958..8920)
                     /locus_tag="PLD53_02015"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRC59710.1"
                     /translation="MKIFVSWSGDFSHAVAKALKDWLPNVIQAVEVFLSSEDIAKGSQ
                     WFHELGKVLDESSFGILCLTRQNLGAPWVLYEAGALGKHFGQARVVPLLIDLKMPDLE
                     GPLAQFNAAELEKDEIAKLVSAINAHLQPAPLSAEKLKKAFKTWWPTLEKSLNQARTD
                     ALPAGKKFTYDVFLSTPMAAFKTDAEYRSGRAEFKKVFDALKQQCALSVYWASEKIES
                     IDDFDTLDVSAMDDLVALKQSHRFVLLYPQKLPTSALFEAGYALALDCDSHYFVRDRD
                     DLPFLMRELAGVTQKVRIHTDTDWKDYDDLSEKMVRYKDKWFGK"
     gene            complement(8943..10382)
                     /locus_tag="PLD53_02020"
     CDS             complement(8943..10382)
                     /locus_tag="PLD53_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HRC59711.1"
                     /translation="MTTCFVVQGFGKKTDFTDGRVLDLDASYAIIKEAVEASNLECIR
                     ADEIPHSGTIDVPMYKLLLQADLVIADLSTYNVNAAFELGVRYGLRPSATIIVAEEGF
                     KNPFDVGHIVIRRYKHLGEDIGAKEAKRFRDDLKQAIADILAAGAIDSPVYTFLPELT
                     PPRVERAGDEIPTPVSASIAPAASPAVPPPASPLAAAPAAGATARGGANLRFDETMAG
                     TAVFRVGQGTATGDDETLPQTAKANLEQAQAKLLAGEFADACRLLKEVQKQRPNDSFV
                     IQQLALSTYKSRQPTPEAALTEARDILLVLNPETTNDPETLGLWGAIHKRLWEISHLD
                     ADLGKSIDAYGRGFQLKQDYYNGINLAFLLELRALTALKAGEQEDGLTDSVLAKRIRR
                     EVIKYAAPLITGDYDYGDDKRYWILATLWEAEAGLGNTTAAESWAAQARALKPAGWMT
                     DSTQTQIQNILTMQTEIARLLSHGSTTRP"
     gene            complement(10382..11569)
                     /locus_tag="PLD53_02025"
     CDS             complement(10382..11569)
                     /locus_tag="PLD53_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRC59712.1"
                     /translation="MSSKLIASIDSVGPKKILTCDGGGILGLISVEILARIEADLRAE
                     LKKPDLVLADWFDFVCGTSTGAIIAACIATGMSIDQIREFYVGSGEQMFDKASVFKRL
                     RYSYNDEPLAEKLRNELNRALGYPEGSPPATLGDDGLRTMLMMVLRNHTTDSPWPVSN
                     NPRAKYNQRIKSDGSPRKDCNLDLPLWQLVRASTAAPTFFPPEVVTFAPGTPDQYQFI
                     FVDGGVTTYNNSAFLAFQMATSAPYKVNWATGTDKLLIVSVGTGNAAKARPDLKEEDL
                     WLLDHAKSIPSALMNAASAGWDTACRILGECRHGGPIDREIGDLVATPDAPNWTGAKQ
                     FAYVRYDPDVSCEGLDALGLKDIEPERVQLMDSIKYITEIQRVGVAYAATHLDLGHFK
                     GFV"
     gene            complement(11654..13585)
                     /locus_tag="PLD53_02030"
     CDS             complement(11654..13585)
                     /locus_tag="PLD53_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC59713.1"
                     /translation="MPIDQTTHDQLERSLQEIDWAALRRPGYLPSPAAWEDQVLYFLM
                     LDRFSDGREQGFRDIHGNIIVTGSTPPFSFFDDAYKADRSSWADTGDEWLGGTLKGLE
                     SKIGYLKRLGATAIWISPVFKQVAADTHSYHGYGIQNFLDIDPHFGSRQDLIDLVNTA
                     HANGIRVILDIILNHSGDVFGYQFNPDRYPARDEHGNIGMDPRWDGGTYPVAGWRNAF
                     GQTNIPFGPINPATHGYAWPNDAVWPAELQDAATFTTKGRINSWDNDPEFLEGDFVTL
                     KDIHHGDHERDAQNRRIVDAFHPTPALTALCDVFKFWIGLADVDGFRIDTVKHMEPGA
                     TRYFAAVIKEFAQSLGKENFFLVGEITGGRDNAYNTLELTGLDAALGIDDIPDRMDYL
                     AKGWREPVDYFNLFRNSALVNKSSHVWFGKHVVTLFDDHDQVRKGNNKGRFCGDWVNN
                     GYAHLKAVLGLNLMSMGIPCIYYGTEQAFDGTGDNDRYLRECMFGGAFGSLQSSGRHF
                     FDENHEIFRFIAEITVLRSQHLALRRGRQYLREISDSGEDGDFGLPHMIGGQIRSVVP
                     WSRLFDNQEILLAINTDAYSARAAWVTIDATLHAEPGMLTCLYSTDVARIGDATTIES
                     RNGRAVHIEVPAGGFVMYG"
     gene            14117..14902
                     /locus_tag="PLD53_02035"
     CDS             14117..14902
                     /locus_tag="PLD53_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRC59714.1"
                     /translation="MLIRTFAATLAALVALPASAYTIDGNLTDWGVQTNFTTGNAIKG
                     HTVDTDSTGNLSQYVNPGWGGQAYDAEALYVDYDSSKLYLALITGHNPAMVNSGSSSY
                     APGDFLIDFGRDGTFEYGIKTTGVDIGKLFKIAGESDLKFGLFTGQGTPYTDGRNAVS
                     IKDGHGQKLGTGGTLAISGAFTGYGAYTGDTHYAYEAAIDISLFGGDWSLPFDVQWTM
                     QCGNDLVLADPPQATVPEPMSIALFGIALAGLGLSRRRNQNSC"
BASE COUNT         3167 a         4156 c         4447 g         3277 t
ORIGIN      
        1 aactccaagt gtttcagttc cagatgggac agacctttta actcgggaga agtaacttga
       61 acacgaaaat tgccacgttg ctggtcgcgt tgatggcttc gctgccagtg tctttcgcgt
      121 tggcatcgga aacagtgaag attggttacg tgaatacgca gcgcatattc cgtgacgcgc
      181 ccacggccgt caaggcagca aagaagcttg aatccgaatt ttcaaaacgc gaccaggatc
      241 tgcagcgcat ggccaagcaa atccagtcct tgcaggaatc actggaaaaa aatgcactta
      301 cgatggttga gaccgagcgc cgtgcgaaag aaaaagacct gaacgagcta actcgagagt
      361 tccagcgtaa acagcgggag ttccgcgaag acctgaacct tcgtcagaac gaagagaatg
      421 ccgccatcat cgaaaaggcg aacaaggcga tcaaacagct tgccgagagc gaaaagtatg
      481 atctgatcgt ccaggatgtt gtctgggtta gcccgaaact cgatgtgaca gacaagatca
      541 tcaaggcatt gtccgaaggc aagtaattag ccgtgcctgc gcaagaaggt ctgcggctcg
      601 acgaaatcgt ttcccgcctt ggcggttcac ttgagggtga cgcgtctgtc attgtgtcgc
      661 aggttggcac tctggcctcg gcgggccccg gacagatagc cttccttgtc aatcccaagt
      721 accggcaaca gttgcagact accctggcgg cagcggttat cgtgccgcct cagtttgccg
      781 gggatacggc gcttccgcgg atcattcatc ataacgccta cgcctattac gctcgggttg
      841 tggctttgct gaacccgagt gcttcgcgtc cggctggtgt tcacccatcc gctgtagtgc
      901 atagccttgt tccctcctcg gccagtattg gcgaaaacgt tgtgattggt caggatgttg
      961 caattggcga aaacgttacc ctgtatcctg gctgcatcat cggtgacggg gtttcaattg
     1021 gcgatgattc gctgttctat ccgaacgttg tgatttgtca ccattgtgtg atcggccagc
     1081 gtgtccttat tcaggctgga gcagttatcg gtagtgatgg ctttggtttt gccagcgagg
     1141 gagagcactg ggtcaagatt ccgcaaatcg gtcgtgtagt cattggtgat gacgtcgaaa
     1201 tcggcgccaa tacgtcgatt gatcgggggg cgctggatga cacgatgatt ggtcacggcg
     1261 tcaaactgga caatcaaatc cagattgcgc acaacgtggt catcggtgat catgtggcga
     1321 tggcgggttg tgttggcatt gcgggcagca caaaaatcgg acgtcgctgc accatcggtg
     1381 gtgccgggat gattactggc catcttgaat tgactgatga tgtccacatt tcagccggca
     1441 cattggtaag caagagcctg cgcaaaccgg ggcaatacac cagcattttc ccgcttgaag
     1501 tgcatgagga gtggctgcgc aacgcggctc agatcaagcg gctggcaaaa ttggccaagc
     1561 gggtttcaga tcttgagaaa aaactcgagg aaatggagaa aacatcttga atgcaatgga
     1621 tattaaggaa attcttgacc acctgccgca tcgctatcca ttccttttgg tagatcgcgt
     1681 gatttcgttc gagcccggga aagcgattca tgcttacaag aacatcacaa tcaatgaacc
     1741 ttattttgtt ggccattttc cgcatttccc ggtgatgccg ggggtgctga tcatggaggc
     1801 gctggctcag gctgccggaa tcctttcatt caagaccatg ggtataaagc cagatgacaa
     1861 ctcggttttc tattttgtcg gcattgacaa tgcccgtttc aagaagccgg tgactgccgg
     1921 cgatcaactc cacttgcatg tcgagatact gcgtcagatg cgtggcatct ggaaatacaa
     1981 ggccgaagca cgcgtagacg gggagatcgt ggccgaagca gagctcatgt gtgccaaacg
     2041 cgacgtggca tgattgcggg gacttggctt tgagtacaga catgattcac cccagcgcaa
     2101 tcatccaccc tggcgcgcgg ctcggttccg gcgtctctgt cggcccttat tcgatcatcg
     2161 gtgagaatgt cgagatcggt gataacacct ggattggtcc gcatgtcgtc cttgccgggc
     2221 atacgcggat tggctgtgac aaccggattt tccagttctg ctcacttggc gagataccgc
     2281 aggacaagaa atatgcgggt gagccaacgt gcctggaaat cggcgaccgc aacaccatac
     2341 gcgagttttg cacgttcaat ctcggtacgg cactggatgc gggagttacg cggctgggtg
     2401 acgacaactg gatcatggcc tacgtacata tcgcgcatga ttgtcaggtt ggaaaccgta
     2461 caaccttcgc caacaacgct cagctcgccg ggcacgtgca cattgacgac tgggccatac
     2521 ttggcggcta taccggggtg caccagttct gccgtgtcgg tgcgcatacc atgacggccg
     2581 tgggtacggt ggtactgcag gacatacctc cgttcgtgat ggctgccggc aatacggcaa
     2641 gcccctttgg tatcaacgtc gagggcttga agcgccgcgg tttctctccc gatgccctga
     2701 aggctctgaa acgagcttat cgaaccctct acaagtcggg gttcatgctc gatgaggcgc
     2761 gcgccaagct cgaagaagag gtcaggtcat accctgaaat ccagccgctt ctcgattttc
     2821 ttgcgatttc caggcgcggc attatccgat gacttcggag gtggttcgta tcgccattgt
     2881 ggcgggcgag gcttcgggcg atcttcttgc tagccacctg attcagacgc tcaaggcgaa
     2941 actaccgggc gcagtgtttt atggcattgg cggtcccaag atggctggtc aaggctttga
     3001 tgtctggtat ccatcggaaa aactcgctgt gcttggctac gcggaagcgt tgaagcacta
     3061 ctttgaaatc tcctcgattc gaagcaagct caagcgccgc ttgctcgctg atccgccgga
     3121 catattcatt ggtgttgacg caccggactt caatctcgcg ctggaaaaat gcatgaaaca
     3181 acacggcatt cccagcgtgc actatgttag tccgtcgatt tgggcctggc gtggacaacg
     3241 aattcacaaa ataggagctg ctgtttcgcg cgtattggcg ctgtttccct ttgagcctgt
     3301 gctgtatgag aaacagggga ttcccgtcag ctacgttgga cacccgctgg ctgacatgtt
     3361 gccgcttgag gatgggcgga aaagtgcgcg cgagctgctt ggcttgtcgt cgcaacggcc
     3421 tgtttttgcc ctcttgccgg gtagtcgaca gtccgaattg aacaatctgg cagaaatttt
     3481 cataaggacg gcgtgcgaaa ttcaccggaa aatgccgaac gcgctttttc ttgtcccttt
     3541 ggcgacgcgc gaaacacgtt tgctgttcga aaccgcgctc tatcgctgtg atgcgcgcga
     3601 agttccgatt agcttgctgt tcggtcacgc gcatgaagcc atgatggctt cagacattgt
     3661 gcttgttgcc agcggaaccg ccaccctcga agcggcactg ctcaaacgcc cgatggttat
     3721 cgcctacaaa atggcacctt tctcctatcg tctgatgcgg cgtatggggt atctgccata
     3781 cattggcttg cccaatatcc ttgccggagg atttgtcgtt cccgaattca ttcaggatga
     3841 cgcgacaccc gaaaacctgg ctcaggcact gctcaatctc tacgccgaca aggggatttg
     3901 cgcgcgtatt ggcgaagtgt ttcgagagat acatctgcaa ctcaggcaga ataatgcaga
     3961 aaaagcggca actgcgattc tggactgcct gccggttgca gtgcgcaacc atgcgtctgc
     4021 tgcagccgac tgaaggcctg gtttgcggcg ttgacgaggc cgggcgcggt ccgttggcag
     4081 gcccggtggt ggccgctgcg gtgattctcg acccggcgtg cccgatcgac ggccttgacg
     4141 actccaagaa gctcacagcc aagcgacgcg aagccttggc agtcaagatt cgcgccaagg
     4201 cgctgagctg ggcaatagcg gaggccagtg tcgaagaaat cgaccgcatc aatatccttc
     4261 aggcaagtct gctggccatg cgacgtgcgg tcgaggcgct gggaataccg ccaagcaacg
     4321 cgctgatcga cggcaaccgc tgcccgcaac tggcgtgtcc tgcgcaggcg attatcggcg
     4381 gtgatggcaa ggtggcctcc attgccgcgg cgtccattct ggccaagaca tttcgcgatg
     4441 ccggaatgct tgagctgcat gcaatttacc cacagtacgg cttcgatcag cacaagggct
     4501 atccaacgcc tgcgcacctt aggaccctgc gggagcacgg cgtaagcgcg gtgcatcggc
     4561 gaagctatgc gcctgtggcc cagttgatac tcctgtgaag catattgctt cgcgtgacaa
     4621 cccaaacttc aaggcactga agaagctcag tcagagcggt cgcgaacgtc gaaagcgtgg
     4681 gcgagccgtg cttgacggca tgcatctgat cgaaagctat atccttcatt tcgggcagcc
     4741 cgaggaagtt gttgtcagcg aaagcggcag cttgcgcagc gaaatttcac ggtatttgac
     4801 gagtggccga tccggaaatg tggtcagcat tctgagtgat gccctgtttg atgaactggc
     4861 gatggtggag acgccaagcg gcattctggc catagttttg caaccatgtc ccgcgcacgg
     4921 cccggatcag ggaattgaca ctgtcctgct tgatggcatt caggatcctg gcaaccttgg
     4981 gtcgatcctg cgaagcgcgg cggctgcagg ttttcggcaa atacttcttt ctgccgaatg
     5041 cgctcaggcg tggtctccca agaccctgcg ctctgcgatg ggggcccatt tccagcttga
     5101 tatccacgaa agctgtgatc tggtcggatt tctggccggc tatcgtggcc aggtggtgtt
     5161 gacggcgctg aatggagctg tcgacctgta ttcgctggca ctgaaagagc cgttggcctg
     5221 ggtttttggc agtgaagggc agggtgttcg gccggaagtg gcggcaactg cacaactgca
     5281 ggttcgcatc ccgatgccgg gagcgaccga atcgctcaac gtggccgcag ccgcggcagt
     5341 atgcctgttc gagaccgtcc gtcagcgtcg atagtgctat tcgagagtgc gccgggcagt
     5401 gaattcaacc ggggagtggg tggacaggcc gcccgtggtt tccagcacca gatgcaattc
     5461 tcccgacttg agctggcccc gatagcgatg ggtttgctcg cgcagggctg aaccgttcat
     5521 cacttcctgg ctatgggtga caaagctcag ttggttgccg cgcagttgcc cttgctcaat
     5581 gatccgcgcc aggctcagaa agctggcagt gccgtgcatt tcgccattct ccagacgtag
     5641 ctcgaagatt tcgtcgtgtt cggcgcccca gccgtacctg accctggctg tccagtgtcc
     5701 ggtcagtcgg ctgtcggttt gctccggaga aggactcgtc ggcagtggcg acggattacg
     5761 cggtgtttcc ttcaaattgg gtatgagcag catgctgaga acgatcacag tcgccaagcc
     5821 ccacgccacg acacgccgca gctgggagcg tctctgagcc ggcagaagtg gccgtatgaa
     5881 ctcgaccaga cgggcgacat cggaatccca gctggcatcg gaaaggacaa atgcctgacg
     5941 aagggcaagc gcagaaattt cattcggcaa atccgccttt tccggcatca gggctccgcc
     6001 gacgagtact ggtagtacgg gtttccccga ttccaatgcg gcctgaattt ctctgcggac
     6061 gaaatcatcg gtttcgttga gccggcgaac gccgttgttg tttacggtca gccaggccgg
     6121 accaatcatg accagcatta cgctaacact gctcaactgc ttctggatgg cgacgacgaa
     6181 atcctcgccg gggtgaatat cctcgacgtc tcgaaataca ttttccacgc cgaaagcgtt
     6241 ctccaggacg tcggcaagtc ggccagcaaa accagcctgg tcgtcacggc gatagttgat
     6301 gaatattcct ggcaaagttc agaccttatc ggaaaacaag gctgacaaca cacggtcagt
     6361 catcgcgctg tccatttgat ccgcagacag ccactcgaaa cgtccggcag cggccgcatc
     6421 gatgtccgac tcggcttcag tacgggctgt gtcacgatcg tacagtacaa cgattcgacg
     6481 cagacgactg tccccggcca gaacgcccag ctcgtatctg cagccgcggt tttccatgcg
     6541 gaatccgcgc aagtccatga ggacgacatc cgcgcgcgca accagcgcat gtagagccga
     6601 ttgccaggta ctgtcgaagc aatagcattc attgatccgg tagcgcccgt ccggatcggg
     6661 ctgccagtcg aacccggcga tgcgttcccc tatttgtgcc gggttggcga tgaagcgctc
     6721 ggccagagta ccgttgagat agacaaagag atcgtccgga tccagggttc ggctgagcaa
     6781 gtcggtgccg gcaatcagca ccgtgttgcc ggatagccgc cagcgctcga taacctgatc
     6841 gaagagccgt tcgacctggg cgtcgcgctg aaaaacacgc aataccaaca gggtgggcgg
     6901 gctgagtcgc ggcttcaagt gtccacgcag gcagaacaac accaggggta tccagagcaa
     6961 agccggaacc tggacgaaag ccagccaacc cacgctttcc atggccggca gtgcgctggc
     7021 aaagagaatc acgaaccagt aggtggcaat caggtagatc agatcggaaa agcgcttcga
     7081 ccgataagcg tttgccagcc atttgccgaa ggagaagaca ggccaggcca ggatcagcca
     7141 gggtccgagg gcgaacagca gcagggtcgg gtatgcaccg agagtcccta ccaggccgat
     7201 gacccaaggg gcaggattat gtgcgctctg cgccatgacc tgcaaagtca gcaccgacga
     7261 gccggtgagc agcacgctcg gcgcgagcag atacggcgcc acagcgcgaa ttcggccgct
     7321 ggcgccgatc agaaagatga tcgtcatcgg gatggcgacc tggctgccca gccagccgct
     7381 cgccgaagcc aggttttggg cttcggtaga acgcgccatg accagcacca gcatcaccag
     7441 catgtacagg atgacgccgc caaggacgcg cagccacgac catcgccgag ccagtcccca
     7501 ggccagcacc atcggccagg cgtagatcgc tcccagcagc agcacgcggt tcaggctcaa
     7561 ctcgccttct tgatagatga aaaacagggc cagataggat tgagtcagtg cgatgagcaa
     7621 ggaaatgccg ctcagaacga gcatcagtcg aacagaggca tggcggttcg tgcccaaatc
     7681 ggctttgttc gtctcggcgg ctgtcacggg ctcgtggtgt gctgccaggt tcagtgtgct
     7741 ctggttcccg cccgtcatcg ccgtcgctgg aggaacgcca ccgcgcatca gcgccagcat
     7801 gccgcgctga tacaaggcag cgaccaccca ggccagcgcc gccgcaagca gaacggcgga
     7861 gatcaggatg aagaagactg cgcctgatgg aatcattttc tgaggtgaaa caccgcttca
     7921 ggctgcaaga aggatccggt cacgcagaat tccggcgcta tttcccgaac catttatcct
     7981 tataacggac catcttttcg gaaagatcgt cgtaatcctt ccagtcggta tcggtatgaa
     8041 tgcgcacctt ctgggtcact cccgccagct cgcgcatcag gaacggaagg tcgtcccggt
     8101 cgcgaacgaa atagtggcta tcgcaatcca gagccagggc atagcctgcc tcgaacaagg
     8161 cgctggtagg cagtttctgc ggatagagca gaacgaagcg atggctttgc ttcagggcca
     8221 ccaggtcatc catggcagaa acatccagcg tgtcgaaatc atcgatactc tcgattttct
     8281 cggaagccca atacacggat aacgcacatt gctgcttcag cgcgtcgaat accttcttga
     8341 attcggcacg cccgctgcga tactccgcat ccgttttgaa ggcggccatc ggcgttgaaa
     8401 ggaatacgtc ataggtgaat ttctttccgg ccggcagggc gtcggtccgg gcctgattca
     8461 gcgatttctc gagcgtcggc caccaggttt tgaaggcttt cttcagtttc tcggcggaca
     8521 ggggtgccgg ctggagatgt gcgttgatcg ccgaaacaag cttggcgatc tcgtcctttt
     8581 caagttccgc cgcgttgaat tgcgccaatg gtccttccag atccggcatc ttcaaatcaa
     8641 taagcagagg aacaacgcgg gcctggccga aatgcttgcc gagtgcgccc gcttcgtaga
     8701 gcacccaggg ggcgccgagg ttctgtcgtg tcaggcacag gattccaaac gaggactcgt
     8761 caagtacctt acccagttcg tgaaaccatt ggctgccctt ggcgatatcc tccgaggaca
     8821 ggaatacttc caccgcctga attacgttcg gtaaccagtc cttgagcgct ttggcaaccg
     8881 cgtggctgaa atcgccggac caactgacaa agatcttcat tgttgttttc cttcaggcac
     8941 gatcaagggc gggttgtcga tccatgactc aggagtcggg caatctccgt ttgcatggtc
     9001 aatatgtttt gaatctgagt ctgcgttgaa tcggtcatcc agccggctgg tttcaaggca
     9061 cgcgcctgcg cggcccagct ctcggcggca gtcgtattgc ccaggccagc ctccgcctcc
     9121 caaagggttg ccaggatcca gtagcgcttg tcgtcgccat aatcgtaatc cccggtaatc
     9181 agcggtgccg cgtactttat gacctcgcgc cggatgcgtt ttgccagcac cgaatcggtg
     9241 agaccgtctt cctgctcgcc tgctttcaac gcggtcaaag cacgcaattc cagaagaaaa
     9301 gccagattga tcccgttgta ataatcctgc ttgagctgaa agcctcgacc gtaagcatcg
     9361 atgctcttgc ccaagtcggc atcgagatgg ctgatctccc acaggcgctt gtggattgca
     9421 ccccacagac ccagggtttc cgggtcgttg gtcgtctccg ggttcaagac cagaagaatg
     9481 tctcgggcct cggtcagcgc tgcttcggga gtaggctgtc tggacttata ggtggacagg
     9541 gccagttgtt ggatgacaaa actgtcgttg ggtcgttgtt tctggacctc tttcaacagc
     9601 cggcaggcgt cggcgaattc gccggccagc agctttgcct gagcctgctc aagattggct
     9661 ttggcggttt gcggaagcgt ttcgtcatcc cccgtggcgg tcccctgacc aaccctgaag
     9721 accgccgtgc cggccatggt ttcgtcgaag cgtaagtttg ccccgcctcg tgccgtcgcg
     9781 ccggctgcag gggccgcagc aagcggagat gcgggcggcg gcacggcagg agaggcggcg
     9841 ggtgcgatgg aagccgatac aggagtggga atctcatccc cggcgcgctc tacccgcggt
     9901 ggggtcagct caggcaggaa agtgtagacc gggctgtcga tcgcgccggc cgccagaatg
     9961 tcagcgatgg cctgcttcag atcatcgcgg aaacgcttgg cctctttggc gccgatatct
    10021 tctcccagat gcttgtagcg gcggatcacg atgtggccga catcgaacgg gttcttgaaa
    10081 ccctcctcgg cgacaatgat tgttgcgctc ggacgcagcc cgtagcgcac ccccagttca
    10141 aaggcggcat tgacgttgta ggtggacagg tccgcgatca ccaggtcggc ttgcagcaag
    10201 agcttgtaca tgggcacgtc gatggtgccg gaatggggaa tctcgtcggc gcggatgcac
    10261 tcaagattcg acgcttcaac ggcttccttg atgatcgcgt aggatgcatc cagatccagc
    10321 acgcgcccgt cggtgaaatc ggtcttcttg ccgaagccct ggacaacgaa acaggtggtc
    10381 atcagacaaa tcccttgaaa tgcccgagat cgaggtgggt cgcggcgtac gccacgccca
    10441 cgcgctggat ttcggtgatg tacttgatcg aatccatgag ctgcactcgt tccggctcga
    10501 tatccttcag ccccagggcg tcaagaccct cgcacgacac gtccgggtca tagcgcacgt
    10561 acgcaaactg cttggcgccg gtccagttgg gggcgtccgg cgtggcgacc agatcaccga
    10621 tctcccgatc gatcgggccg ccgtggcggc attcgcccag aatacggcag gccgtatccc
    10681 aaccggccga agcagcgttc atcaaggcag acgggatgct ctttgcgtgg tcgagcaacc
    10741 acaaatcctc ttctttgaga tcgggtcgcg ccttcgccgc gttgccggtg cccaccgaga
    10801 cgatcagcag cttatctgtg cctgtcgccc agttcacctt gtagggcgcc gaagtggcca
    10861 tctggaaagc caggaaggcc gaattgttgt aggtggtgac gccgccatcg acaaaaatga
    10921 actggtattg gtccggcgta cctggcgcga aggttacgac ctcgggcgga aagaaagtgg
    10981 gcgccgcggt gctggcgcgc accagttgcc acaaaggcag atccagattg cagtccttgc
    11041 gcgggctgcc atcggatttg atccgctggt tgtatttggc gcgcgggtta ttgctgaccg
    11101 gccagggtga atcggtcgtg tgattgcgca gcaccatcat cagcatggtg cgcaacccgt
    11161 catcacccag ggtggcaggc ggcgaaccct cgggatagcc gagcgcacga ttgagttcgt
    11221 tgcgcagttt ttcggccagg ggttcgtcgt tgtagctgta gcgcagccgc ttgaaaaccg
    11281 aggccttgtc gaacatctgc tcgccgctgc cgacatagaa ttcgcgaatc tgatcgatcg
    11341 acattccggt cgcgatgcac gccgcgatga tggcgcccgt gctggtgccg cagacgaagt
    11401 cgaaccagtc ggccagcacc aggtcgggct ttttcagttc ggcgcgcaga tcggcttcga
    11461 tcctggcgag aatttcaacg ctgatcaggc caagaatgcc tcctccgtca cacgtcagga
    11521 ttttcttggg gccaacggaa tctatgcttg cgatgagttt gctggacatg cttgcctcct
    11581 tattgatgtg ctgatgctac ctttttagct gataaccacg tcccgttcgc cgtggtcaat
    11641 ggagatgcaa ttctcatcca tacatcacga atccacccgc cggcacttcg atatgcaccg
    11701 cccggccgtt ccgcgactcg atggtcgtgg cgtcgccgat acgcgccacg tcggtagagt
    11761 agaggcaggt cagcatgcca ggttcggcgt gcaaggtggc gtcgatggtg acccaggcgg
    11821 cacgggcgct gtatgcatca gtgttgatgg ccaacaggat ttcctggttg tcgaagaggc
    11881 gcgaccaggg aaccactgag cggatctggc cgccgatcat gtgcggcaga ccaaagtcgc
    11941 catcctcgcc cgaatcggaa atctcccgca ggtattgccg tcctcggcgc agcgccagat
    12001 gctggctgcg taataccgtg atctcggcga tgaaacggaa gatctcatga ttttcgtcga
    12061 aaaaatgccg gccgctgctt tgcagggagc caaatgcacc gccaaacatg cattcgcgca
    12121 ggtagcggtc attgtctccg gtaccgtcga aagcctgctc ggtaccgtag taaatgcagg
    12181 gaatccccat ggacatgagg ttcaggccga gcacagcctt gagatgagca tacccgttgt
    12241 tgacccagtc gccgcagaaa cggcccttgt tgtttccttt gcgcacctga tcatgatcgt
    12301 cgaacagggt aacgacgtgt ttgccgaacc agacgtgaga ggatttattc accagcgccg
    12361 agttgcggaa caggttgaaa taatcgactg gttcgcgcca gcccttggcc agatagtcca
    12421 tgcggtccgg aatgtcgtct atgcccagcg cggcatcgag tccggtaagt tccagtgtgt
    12481 tgtaagcatt gtcgcgccca ccagtgatct caccgaccag gaagaagttt tccttgccga
    12541 gactctgtgc gaattccttg atgacagccg cgaaatagcg cgtcgcgccg ggctccatgt
    12601 gtttgacagt atctatgcgg aagccgtcaa cgtcggccag gccgatccag aatttgaata
    12661 cgtcgcagag ggcggtgagg gcaggcgtgg ggtgaaaggc atcgacaatg cgccggtttt
    12721 gcgcatcgcg ttcgtgatcg ccatggtgga tgtccttgag ggttacgaag tcgccctcga
    12781 gaaattccgg atcgttgtcc cagctattga ttcgcccctt ggtggtgaag gtcgcggcgt
    12841 cctgcagctc tgccggccag actgcatcgt tgggccaggc ataaccgtgg gtcgcaggat
    12901 tgatgggccc gaaagggata ttggtctggc cgaaggcgtt acgccagccg gccaccggat
    12961 aggtgccgcc atcccagcgc ggatccatgc cgatgttgcc gtgctcgtcg cgggccggat
    13021 aacggtccgg attgaactga tagccgaaca catcgccact gtgattgagg atgatgtcga
    13081 gaatgactct aatgccgttt gcgtgagccg tgttgaccag atcgatgagg tcctggcggg
    13141 agccgaaatg ggggtcgatg tccaggaagt tctggatgcc atagccgtgg taggagtggg
    13201 tatccgcggc gacctgcttg aaaaccgggc tgatccagat ggccgttgct cccaggcgtt
    13261 tcaggtaacc gatcttgctt tcgagtccct tcagcgtacc tccaagccac tcgtcgccgg
    13321 tatctgccca gctggaacga tccgctttgt aagcatcatc gaaaaagctg aacggggggg
    13381 tactgccggt gacgatgata ttgccatgga tgtcacggaa gccttgctcc cgtccgtcgg
    13441 aaaagcgatc gagcatcaga aagtagagca cctggtcttc ccaggcggca ggtgacggaa
    13501 gatagccggg ccggcgcaag gcggcccagt cgatttcttg cagactgcgt tcgagctggt
    13561 cgtgagtggt ctggtcgata ggcatggtgg cttacctcaa tgaatgtatg tcatgtgagt
    13621 tgtatgggct ggctggccgt catggggctt tgttgatcgt tttgaattca cccttcactt
    13681 cactctgttt ttcctttcgg cattccggag tgcaaccttg tatgaagcag actagggcag
    13741 tgcttattga gttccgtgaa atccggttgc catgggctca cgtctaccac gtccgtcgga
    13801 atgcgcctga ccagacggcc aaggcgttgc taccccgttg gatgaagcca agtgtcggat
    13861 ttttttacac aaaatcgata ccgatgaatc gttctattcg taatagccta atggaaatcg
    13921 agggtttttc aatcaattcc gtgaattagt tcacgggcgt caatcggcat gcggaactat
    13981 gtcacgcttt ttgccgaatt cgctcgtgtc ggggaaattt acatgtgcag aaaggaacca
    14041 gggtcggact caatgaaacg gcgtatggca tgcatattgc agttacaacg ctacatacaa
    14101 ctggagtctt caaatcatgc ttattcgtac gtttgccgca accctcgctg ccctggtcgc
    14161 cttgcccgcc tcggcctaca ctattgatgg caatctgacg gattggggtg tgcaaaccaa
    14221 cttcaccacc ggcaacgcga tcaagggtca tacggtcgat accgattcga ccgggaatct
    14281 ctcccagtat gtcaatcctg gatggggcgg tcaggcctac gatgccgaag cgctctatgt
    14341 cgattacgac agcagcaagc tctatctggc gctgatcacc ggccacaatc cagcgatggt
    14401 gaatagtggt tcaagttcgt atgcgcctgg ggatttcctg attgattttg gccgtgacgg
    14461 aacgttcgaa tatggcatca agacgacggg tgtcgacatc ggcaagcttt tcaagattgc
    14521 tggcgagagt gatctcaagt ttggtctttt caccggtcag gggacgccct atacagacgg
    14581 tagaaacgcc gtttcgatca aggacggtca tggccagaag cttggtaccg gcggcacgct
    14641 cgccatcagc ggagcattta ccggctatgg cgcctacaca ggcgacacgc actatgccta
    14701 cgaggctgcg attgatatca gcttgttcgg aggtgattgg agtttgccct ttgacgtgca
    14761 gtggacgatg cagtgtggca acgacctggt tttggccgac ccgcctcagg ctactgtgcc
    14821 ggagccgatg agtattgccc tttttggcat cgcgctggcc gggctgggtt tgagtcgccg
    14881 tcgcaaccaa aatagctgct gaatcagcgt aactcggcca caacacgcag aattggattt
    14941 caatatcgcg gccggctcag cgttctgtca cttcttcgcc caatgccttc actgcggttt
    15001 cgaaccaact tgtgaagtcc tgattgagca acagacggtg tgcgtcc
//
LOCUS       DAORPM010000021        14904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_799_length_14904_cov_10.323995, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000021 DAORPM010000000
VERSION     DAORPM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_799_length_14904_cov_10.323995"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLD53_02040"
     CDS             <1..297
                     /locus_tag="PLD53_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59715.1"
                     /translation="DDSITLIEVNKAMERRVNAAMGAGDAWKNPAMPAEIGSEQTATV
                     IEAEPTAQFSSKQEARRYHRYKYYESLAEEQEASRNRGESGLPKAPPNLLQKSF"
     gene            complement(355..1368)
                     /locus_tag="PLD53_02045"
     CDS             complement(355..1368)
                     /locus_tag="PLD53_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59716.1"
                     /translation="MRYLIVALIVTLVGCATPEPTAIQTPEIRVSESTWWAIDEDIDA
                     AALAATGPARNYARGYMGSWVSLVRQRSESDFIPWYTSYWTQQWLAIKVGWYTLSGGE
                     GDDSAKKRLTSYLQAQYLERVLDTVAEEINPEVVRENVLTLYIRLLGEQLQEIRRYYG
                     VPQDQFDRRLEAISSIAQDPPAAHSASLYQLVHAHPITSLPAYAALIARSREAADGAG
                     PRPSGDSLLPVAEQAAETLVAKLALSGGAGVAAAAVGGAAGLAISLGVAGFGAIAHES
                     DRPEMEAQVRENLNSALDEMWHSLMDDPVTGVMAGVYHIYEQIDRSLTAAHARPVESE
                     SER"
     gene            1899..2864
                     /locus_tag="PLD53_02050"
     CDS             1899..2864
                     /locus_tag="PLD53_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRC59717.1"
                     /translation="MKKLTMLTAALAISASFASPAAFAQQKFVTIGTGGVTGVYYAAG
                     GAICRLMNKDKAKHGMRCSVESTGGSVYNVNTIKAGELDFGVAQSDVAYNAMKGENQF
                     KGAPFADLRSVFSIHPEPLVVLARKDANIVSLADFKGKRFNIGNPGSGQRATMEVLLA
                     EMKMKLSDFSLVSEMKADEHGPALCDNKIDGFAYVVGNPSANIQDPTTTCGAQLVNII
                     GPAVDKLVKTYPYYAAVTIPGGMYAANPNPTNTYGVVAAFVSSSKVPNEVVYTMVKSV
                     FENLDDFKKLHPAFAHLDPKEMIKNGLSAPLHDGAVKYYKEKGWM"
     gene            2971..5577
                     /locus_tag="PLD53_02055"
     CDS             2971..5577
                     /locus_tag="PLD53_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRC59718.1"
                     /translation="MAEKETAPVLTDEQLKQLVAEADTGGRKPTGAVAKLIMLTALTW
                     SLFQLWIASPLPYSLGVFVLNDTESRSVHLAFAMFLAFLAYPAFKRSPRDYVPAFDWI
                     LALVGVFCASYLFVFYSELATRPGQPTTLDLATSVMGLILLLEAARRALGLPMVILAT
                     VFIGYIFLGPYMPELIAHKGASLGKGMSHLWLSTEGVFGVALGVSSGFIFLFVLFGAL
                     LETAGAGNYFIKSAISLLGHLRGGPAKAAVVASACTGLISGSSIANVVTTGTFTIPLM
                     KRVGYREDKAAAVEVAASVDGQIMPPVMGAAAFLMVEYVGIPYTQVLKHAILPALISY
                     IALFYIVHLEAMKADIRGIPHKHENSFRDRMLSFLMTVSGIILLAGIVYYGFGWLKDV
                     AGDASLYIVGVVMLATYVGLLKFGTRYPELHMDDPNSPIETLPDPGPTLKSGLHFLLP
                     VVVLIWNLMVEELSPALSAFWATIFLMFILVTQRPILAHFRQHSDIGGALRQGFQDLL
                     DGLVTGARNMIGIGIATAAAGIIVGTVTLTGIGLVMSELVELISGGNLMIMLGLVAVI
                     CLILGMGLPTTANYIVVSTLMAPVVVELGAQSGLLVPLIAVHMFVFYFGLMADVTPPV
                     GLASYAAAGIARCDPIKVGVTAFGYSIRTAILPFMFIFNTQLLLIGIDSVWHLILTVG
                     TAIIANLVFAAATQGWFMTRNRLWEAAVLLLVTFTLFRPGFWMDMIHPPFDKIPATRI
                     MEVVEQAPADDRLRVWIEGEDLNGKEVSKGVLLPLGEKAPALQRLNAIGLSLMPTDDS
                     VDIMGVKFGSRAAKLGIEQGFKITALEVAADRPDKEWLFIPAFVLLALIVFIQRRRVQ
                     LSTEPSKIWRSV"
     gene            5968..10782
                     /locus_tag="PLD53_02060"
     CDS             5968..10782
                     /locus_tag="PLD53_02060"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRC59719.1"
                     /translation="MLDHLEERKQDILAAILKLAAGRLPAPQLDEAQTFIRVYYEQMD
                     PEDLFSRSIEDLYGAAMAHLSYGRSFASGKPKLRAYNPQLEEHGWTSSHTIVEIINDD
                     MPFLVDSVTMEVNRQGYTLQLLIHPVLRTHRDARGKLQAIGATGTDGNAESFMHVEID
                     SETDPVRLKTLVDGLLSVLADARAAVTDWQPMLEQMSTVIKELDSPPVGLSEAEVAEG
                     RIFLNWVKDHHFTFLGFREYELTSENGVDQLRIVPESGLGILREPRQGGLSTSFAESP
                     PGIRKLAREPHLLVLTKANVRATVHRPGYLNYIGIKRFDSAGGVVGERRFIGLYTSSA
                     YYARIREIPLLRNKATAVLEKAGFAPNSHMGKSLLTILEGYPRDELFQIDETTLMETA
                     LGILRLGDRRKIRVFLRREVYGRYYSCLIYLPREKFNTESRIRIQEVLKRALQGSSAE
                     YTVELSEATLARIHFLVRSSPRDESVIDPRDIESEIILALRDWADDLKLALIESRGEE
                     AANHLMTTYCAAFPAAYRGTVSVRIAVRDIQTIEQLSSAQPITMRLYRPVEADSQALR
                     LRVYRLGEPVPLSGSLPMLENMGVSVLDEQSYTLDRGDAGKVHIHDFGMRTVSELPDI
                     STVRPLFEDGFAKVWRREIESDGFNRLVLAAGLSSDEVVVLRAFAKYLRQAGFTFSQA
                     YIEQTLVDNTRLTRLLVALFHVRFDPAARGDRDAAQSTLVEEIKEALDEVASADDDRI
                     IRRFLAVIQATIRTNFYQRDGAGCRKPYVSFKLESAKVPELPEPKPLYEIWFYSPRVE
                     GIHLRGGKVARGGLRWSDRMEDFRTEILGLVKAQMVKNVVIVPVGSKGGFVLKNPPPQ
                     TDREAYLNEGIECYKSFLRGLLDVTDNLVANELVPPKNVVRHDGDDPYLVVAADKGTA
                     TFSDYANAVSEEYGHWLGDAFASGGSVGYDHKKMGITARGAWESVKRHFLEMGIDTQT
                     TAFTVAGVGDMSGDVFGNGMLLSRHIKLLAAFDHRHIFLDPNPDTEVSFNERQRLFVL
                     PRSSWDDYDKSLISHGGGVYPRSAKSIPLTEEVRQILGVSADALTPVELMRAILKAPV
                     DLFYNGGIGTYIKSREQSNAEVGDRANDAVRVNGADLRCKVVAEGGNLGCTQLGRIEF
                     AMNGGRINTDAIDNSAGVDCSDHEVNIKILLGLIEADGELTKKQRNKLLAEMTDEVGE
                     LALADNYYQTQSQSVTGTRAVKMLDGHQRFIRHLEKAGRLDRAVEYLPTDEQIAERRA
                     ARSGLTAPERAVLLAYSKMVLFDQLLESDLVDDPYVAPALTGYFPKVLQARYANAMLR
                     HPLKREIVATVLTNSMINHVGIVFVFRMCDETGAEAHEVVRAYILARDIFDFPALWAE
                     IEALDGKVAAPVQNRMLIDAGRLVLRTVLWFLRTRHGKLPFSEIIELFRPGVAQLGSK
                     LSAFLNVGDLNALNERRAELEAAGVPAGTAEQVAKLDPSYAALDIVDIATDSEQGVDR
                     VAEIYFALVGKLEMRWFGDQINALSANTHWQGLARNALRDDLARQTRLLTASVIRLSP
                     GGTDAAEMLAAWEASNHAPLSRLREMVADLKTGPALDLAMLSVAMRELRSLT"
     gene            10812..11276
                     /locus_tag="PLD53_02065"
     CDS             10812..11276
                     /locus_tag="PLD53_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59720.1"
                     /translation="MQNKRPDRLYSAPTGFIYCNHLLRSAIHGLGGRGARPQILLTTV
                     KDEEHMLGLLMVEALLAASGAWCLSLGAQTPIADIVGAARGTTIDIVAVSSSGAYPAR
                     SESQSALKNYLTVFIKLSKLRLSQGGVALCRGVVNTEILVVEGFPGAPGACR"
     gene            11424..12401
                     /locus_tag="PLD53_02070"
     CDS             11424..12401
                     /locus_tag="PLD53_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRC59721.1"
                     /translation="METIGTWWMWAGFLAIVLVMLAIDLFLVGGGKQHKVSFREAATW
                     SVIWVSVSLLFAAALWWHLDGSAGREVANQKSLEFVTGYLIEKSLAVDNVFVWLMLFS
                     FFGIPLELQKRVLVLGVLGAIVLRTVMIFAGAWLITQFHWMLYIFGAFLLVTGIKMWW
                     FAEEKPDMANNPLIRWLRGHMKISDELHGEKFFVWKDGVRYATPLFLTLILVEISDLI
                     FAVDSIPAIFAITADPFIVLTSNVFAILGLRAMYFLLADMADRFSLLKYGLAAVLVFI
                     GVKMLLIDLYKIPIGFSLAVVATFIGISIWMSLRKEKLARAAAAGRARE"
     gene            12411..13238
                     /locus_tag="PLD53_02075"
     CDS             12411..13238
                     /locus_tag="PLD53_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRC59722.1"
                     /translation="MDIVMHTPGGWRPRLDRWLESAPIHNSLIALILINAVILGLETS
                     PALMAQWGSWLSAADRAILAVFLVEIALRLLAHRLVYFRDPWNVFDFTVVAIALIPAS
                     GPLAVLRALRILRVLRLITLVPSMRRVVGGLLAALPGLGSVVGIICIIFYVAAVIATK
                     LFAADFPALFGDLGSSAFTLFQVMTLEGWAMEVVRPVMAVYPSSWIFFLIFILASTFT
                     LLNLFIAVIVNAIHQEGAEDRRDTTAAEIAGLRNEIAALRSHLGPMRRSSPYATRRR"
     gene            13239..13772
                     /locus_tag="PLD53_02080"
     CDS             13239..13772
                     /locus_tag="PLD53_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRC59723.1"
                     /translation="MMEPTRLADWDHAGLALAHAARSPWLDSLFATVTWLGSLAVLVL
                     LALLLGWRRCGHRNAAFVTLALIGASALGHLVKLIAARPRPDLFPPLIPMPDDASFPS
                     AHAMQVTAFALAWLLRPGASPGRIEIAVLLAAASLVGASRLYLQVHFPSDVVAGVMLA
                     TLWVVLLRRLPAWRENS"
     gene            13772..14173
                     /locus_tag="PLD53_02085"
     CDS             13772..14173
                     /locus_tag="PLD53_02085"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRC59724.1"
                     /translation="MRHKFLGTGDAGYHPLRKIRTAISGLRYAVLYDWSVTYKLIASV
                     VVLAVAFAARAWVDFLLILVVTGFVLVAEIFNSAIEALCDFVETRHNEKIKVIKDIAA
                     AGVGIAIFVWFIVLGVELGRFLDAPLPWMRL"
     gene            14669..>14904
                     /locus_tag="PLD53_02090"
     CDS             14669..>14904
                     /locus_tag="PLD53_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC59725.1"
                     /translation="MHKRRTKIDFAHCMRHIVQTYPDAEVIRVVLDNLNTHKPASFYE
                     AFPPDEARAIAKRLEFHYTPKHGSWLNIAEIELAV"
BASE COUNT         2885 a         4506 c         4404 g         3109 t
ORIGIN      
        1 gacgacagca ttaccttgat tgaagtcaac aaagccatgg aacgccgagt aaatgccgcc
       61 atgggcgcag gagacgcctg gaaaaaccct gcgatgccgg cagaaatcgg tagcgaacaa
      121 acggctacgg tcatcgaagc agaaccgacg gcgcaatttt ccagcaaaca ggaggcccgc
      181 cgttaccatc gctacaagta ttacgaatct ctggccgaag aacaggaggc ttcacggaat
      241 cgcggcgagt cggggcttcc gaaagcaccg ccgaatctgc tccaaaaatc cttctgaata
      301 ccgtgcgaat agacgtcgca tcgatttcct cttggtattt gtcgcccctg atcctcaccg
      361 ttctgattca gattcaaccg gccgcgcatg cgctgcagtg aggcttcgat caatctgttc
      421 atagatgtga tacacgccag ccatcacccc tgtgacgggg tcatccatca ggctgtgcca
      481 catttcatcg agcgccgaat tcaggttttc ccgaacctgc gcttccatct ccggccgatc
      541 gctctcgtgc gcgatagcgc cgaaaccggc cacacccagg gaaattgcca agcctgcggc
      601 accgccgacc gccgccgccg caacgcctgc accgccactg agggcgagtt tggccaccag
      661 tgtttcagcc gcctgttcgg ccaccggcaa caaactgtcg cccgacggcc tcggtccggc
      721 gccatcggcg gcctcgcggc tgcgggcaat cagtgccgcg taggcgggga gactggtaat
      781 cgggtgagca tggacaagct ggtaaagcga agcactgtgt gcggcaggag ggtcctgcgc
      841 aattgacgat atggcttcaa gccgccgatc gaattgatcc tggggcacgc catagtatcg
      901 tctgatttcc tggagttgtt cgccgaggag ccgtatgtaa agcgtcaata cattctccct
      961 gaccacctcg ggattgatct cctcggcgac ggtatccagc acacgctcca gatactgcgc
     1021 ctgcagatag ctcgtcagtc gtttcttggc cgaatcatct ccttccccgc cgctcagcgt
     1081 gtaccagccc accttgatag ccagccattg ctgggtccag tagctcgtgt accacggaat
     1141 gaaatccgat tcggagcgct ggcggacgag ggacacccag cttcccatat agccacgtgc
     1201 ataattcctt gccggtcccg tcgccgccag tgctgcggca tcgatgtcct catctatcgc
     1261 ccaccaggta ctctcggaga cacgtatttc cggagtctgg atggcagtcg gttctggagt
     1321 ggcgcaaccg acaagggtca cgatcagggc gactatcagg taacgcatgt tcaagaacgc
     1381 tgccgagtca attgattccg ctggcggttg ccgacccacc atcgtattgg gcaacactcc
     1441 tcctggcaat tcatatctgg cgtttcgccg ccataccaat cgcccgtcga cgagcatttt
     1501 ccaaagcccc caagttcctt ttttaaagta taggcggtaa agacgtgctc acgaaaatcc
     1561 agcacgaaga ctcaagcctt taggccgggt cttggcgccc ggaagaactc attgaagcat
     1621 cgagaatgcc aaattggtat cagtggtatt cttgaaattg gtattgcaca aaactattat
     1681 ctgtgtagag tgtgaatctt gggtgttgac ttccgtcgcc tgcaggtcgt gttgtttccc
     1741 ctgaagattg acgttggcca acatcacaag cggagctaga actcgctgac gtagaacttc
     1801 ccgggcaatt cgcactccta ccgacgtagt cccgcaatcc gtaggcagtc atcatttgct
     1861 tggttcttat ctgaaaacta taacgaagga gacttacgat gaagaaactc acaatgctca
     1921 ccgccgctct ggcgatttca gccagcttcg ccagcccggc ggcctttgca cagcaaaaat
     1981 tcgtcaccat cggtaccggc ggcgtaaccg gcgtctacta cgctgcgggt ggcgcgatct
     2041 gccgcctgat gaacaaggac aaagccaagc acggtatgcg ctgttcggtc gaaagcaccg
     2101 gcggttcggt atacaacgtc aataccatca aggcgggcga actcgacttc ggcgttgccc
     2161 agtctgacgt cgcatacaac gcgatgaagg gcgaaaacca gttcaagggc gctccgtttg
     2221 ccgatttgcg ttccgtattc tcgattcatc ccgagccgct cgtcgtactg gcccgcaagg
     2281 acgccaatat cgtgtcgctg gccgacttca agggcaagcg cttcaatatc ggcaatccgg
     2341 gctccggcca gcgcgcgacg atggaagtgc tgcttgccga aatgaagatg aagttgagcg
     2401 atttctcgct ggtttccgag atgaaggctg acgaacacgg cccggccctt tgcgacaaca
     2461 agatcgacgg ctttgcctac gtcgtcggca acccgtcggc aaacattcag gacccgacca
     2521 ccacctgtgg tgcccagttg gtcaacatca tcggcccggc cgtagataaa ctggtcaaga
     2581 cgtatccgta ctacgctgcg gtaacgattc cggggggcat gtatgcggcc aatccgaacc
     2641 cgacgaatac ctacggcgta gtggctgcct tcgtctcgtc gtcgaaggtg ccgaacgaag
     2701 tcgtgtatac gatggtgaaa tccgtgtttg aaaatctgga tgacttcaag aagctgcatc
     2761 cggctttcgc tcacctggac ccaaaagaga tgatcaagaa cggcctttcg gcaccgctgc
     2821 atgacggcgc cgtgaagtac tacaaggaaa aaggctggat gtaaaacggt cgtctcatcc
     2881 cggaatcgct agaactccag ccttcccggg gaaacctgca gacggccccg ctcggggccg
     2941 tctgtccttg aagtctgcgg aggtgcgcat atggctgaaa aagaaacagc tccggtcctg
     3001 accgacgagc agctcaaaca actggttgcc gaagccgaca ctggcgggcg caagccgacc
     3061 ggcgccgtcg ccaaactgat catgctgacc gccctgacgt ggtcgctgtt tcaactttgg
     3121 atcgcctccc cgctgcccta ctcgctgggc gtttttgtcc tcaacgacac cgagtcgcgt
     3181 tccgtccacc tggccttcgc catgttcctc gcttttctcg cctacccggc cttcaagcgt
     3241 tcgccgcgcg attatgtccc ggccttcgac tggatactgg ctctggtcgg cgtcttctgc
     3301 gcctcgtatc tcttcgtctt ctactccgag ttggccacgc gccccggaca accgacgaca
     3361 ctggacctcg ccacctcggt tatgggcctc atactgctgc tcgaagcggc acggcgcgcg
     3421 ctcggcctgc cgatggtcat cctcgccacc gtctttatcg gctacatttt ccttgggcct
     3481 tacatgccgg aattgatcgc gcacaagggc gcctcgctcg gcaaaggcat gtcacatctg
     3541 tggctaagca ccgagggcgt gttcggtgtc gcgctcggcg tctcctccgg gttcatcttt
     3601 ctcttcgttc tgttcggcgc gctgctggaa accgccggtg ccgggaacta tttcatcaag
     3661 tccgcaatct cgctgctcgg acacctgcgt ggcggcccgg caaaagcggc agtggtcgcc
     3721 tcggcgtgca ccggcctgat ctccggatcg tcaatcgcca acgttgtcac cactggaacg
     3781 ttcaccattc cactgatgaa gcgggtcggc tatcgtgagg acaaagccgc cgcagtcgag
     3841 gtcgcggctt cggtcgacgg ccagatcatg ccgccggtga tgggagctgc cgccttcctg
     3901 atggtcgagt atgtcggtat cccctatacg caggtgctca agcatgcgat cctgccggcc
     3961 ctgatctcct atattgcgct gttctatatc gtgcacctcg aagcgatgaa ggcggacatc
     4021 cgtggcatcc cgcacaagca cgagaattca ttccgcgacc gcatgctttc cttcctgatg
     4081 accgtctcgg gcatcattct tctcgccggc atcgtctact acggcttcgg ctggctcaag
     4141 gatgttgccg gcgacgcctc gctgtacatc gtcggcgtcg taatgctggc cacctacgtc
     4201 ggtctcttga agttcggtac gcgctatccg gaactgcaca tggacgaccc gaactcgccg
     4261 atcgaaacac tgccggaccc cggcccgact ctgaaatccg gattgcactt cctgctgccc
     4321 gtggtggtgc tgatctggaa cctgatggtc gaggaactct cgcccgcgct gtcggccttc
     4381 tgggcgacaa tcttcctgat gttcatcctg gtcacgcagc gaccgatcct cgcccacttc
     4441 cgccagcaca gcgacattgg cggcgcgctt cgtcagggat tccaggatct gctcgacggg
     4501 ctggttaccg gggcccgcaa catgatcggc attggcatcg ccacggccgc cgccggcatc
     4561 atcgtcggta ccgtcacact gaccggcatc ggcctggtga tgagcgaact ggtcgaattg
     4621 atctcgggcg gaaacctgat gatcatgctc ggactggtcg ccgtgatctg cctgattctc
     4681 ggcatgggcc tgccaacgac ggccaactac attgtggtgt cgaccctgat ggcgccggtg
     4741 gtggtcgaac tcggcgcgca aagcgggctg ctggtgcccc tgattgccgt gcatatgttc
     4801 gtcttctatt tcgggttgat ggccgacgtc acgccacctg tcggcctcgc gtcgtatgcc
     4861 gcagccggga tagcccgatg cgacccgatc aaggtgggcg tcaccgcgtt cggctacagc
     4921 atccgaaccg cgattctgcc tttcatgttc atcttcaaca cgcaattgct gctgatcggc
     4981 atcgacagtg tctggcatct gattctgacg gtgggtacag cgatcattgc caatcttgtc
     5041 tttgccgccg cgacgcaggg ctggttcatg acgcgcaacc ggctgtggga ggcggcagtg
     5101 ctgctactcg taaccttcac gctgttccgt ccgggattct ggatggacat gatccatccg
     5161 cctttcgaca agatacccgc cacccggatc atggaggtcg tcgagcaggc gcccgccgat
     5221 gatcgcctgc gggtctggat cgaaggcgaa gacctgaacg gcaaggaggt ctcaaaggga
     5281 gtgctgctgc cgctcggcga gaaggcgccg gcgcttcaac gccttaatgc catcggtctg
     5341 agtctgatgc caaccgatga cagcgtcgat atcatgggcg tcaagttcgg cagccgtgcc
     5401 gcgaaactcg gtattgaaca ggggttcaag atcacggcgc tggaggtagc ggctgatcgg
     5461 cccgacaagg aatggttgtt cattcccgct ttcgttctgc tggcactaat tgtattcata
     5521 cagcgacgcc gcgttcagct aagcactgag ccgagcaaga tttggcgttc ggtgtaagca
     5581 gatccgcttg cacctagcct gcgttttgtc cgcgctgaat ccggcgttcg attgcggtgc
     5641 acaatttgtc ggcagcagtt ccgatcatct tggcgtcaat cgcggcgaaa ccgagaacca
     5701 ttccggatcg ccgctgagat ggccagctgg aataagcgtg tggcgaacat tggtgacgct
     5761 ggatcaaaat cggcgaatca agccatgggg gcgacagaaa atcgttgccg atcatgcgca
     5821 caacgaatca tggcagcagg catttctcag cagctgcgat cttcgatttg ttctgcccgg
     5881 aaggccggtt ttggcaagcg caattgattg gggccggagt cacttcagcc tgcacgcctg
     5941 tcgcgtaaat cgcaaaaagg aggagctatg ctggatcatc tcgaggaaag aaaacaggat
     6001 attcttgccg cgattctgaa gttggccgcc ggccggctgc cggcgccgca acttgacgaa
     6061 gcgcagacct tcatccgtgt ctactacgag cagatggatc ccgaggacct gttttcgcga
     6121 agcatcgaag acctctatgg cgcggccatg gcacatctct cctacgggcg atcctttgcc
     6181 agcggcaagc cgaaacttag ggcctacaac ccgcaacttg aagaacacgg ctggacgtcg
     6241 tcgcatacga ttgtcgagat catcaatgac gacatgccgt tcctggtcga ttccgtaacc
     6301 atggaggtta accggcaggg gtacaccttg cagctcctca ttcacccggt gttgcgaact
     6361 catcgcgatg cgcgcggcaa gctgcaagcg ataggagcga ccggaaccga tggaaacgcg
     6421 gaatcgttca tgcatgtaga aatcgacagc gagaccgatc cggtgcggtt gaagacgctg
     6481 gtcgatggtt tgctctctgt ccttgccgac gcgcgcgctg cagtgaccga ttggcagccg
     6541 atgctcgaac aaatgagcac cgtcataaag gaactggatt caccccccgt cggcctgagc
     6601 gaagcggaag ttgccgaagg tcgcatcttt ctgaactggg taaaggatca ccacttcacg
     6661 tttcttggct tccgcgagta cgaactgacg tcggagaacg gagtggatca gctcaggatc
     6721 gttcccgaga gtggactggg catcctgcgc gaaccccgcc aagggggttt gtcgaccagc
     6781 ttcgccgaat cgcctcccgg cattcgcaaa cttgcgcggg aaccgcattt gctggtcctg
     6841 accaaagcca atgtgcgggc gacggtacac cgccccggct acctgaatta catcggcatc
     6901 aagcgcttcg actcggcggg cggtgtcgtt ggcgaacgcc ggtttatcgg cctctacacg
     6961 tcctctgcgt attacgcacg gatcagggaa attccactgt tgcgaaacaa ggcgacagcg
     7021 gtgctggaaa aagccggttt cgcgcccaac agtcatatgg gcaaaagcct gttgaccatt
     7081 ctcgaagggt atccacgcga cgaattgttt cagatcgacg aaaccaccct gatggaaacc
     7141 gcgttgggca ttctgcgtct gggagaccgc cgcaagattc gcgtcttcct gcgccgtgag
     7201 gtgtatggcc gctattactc ctgcctgatc taccttcctc gcgagaagtt caataccgag
     7261 tcccgaattc gtatccagga ggtgctgaag cgcgcgcttc agggcagcag cgccgagtac
     7321 acggttgaac tgtcggaagc cacgctggcg cgtatccact ttctggtgcg cagttcgcca
     7381 cgcgatgaat cggtcattga cccgcgcgac atcgagtcag agatcattct cgccttgcgc
     7441 gactgggctg acgacctgaa actggccctg atcgaaagcc gcggcgagga agcggcaaat
     7501 cacttgatga cgacctattg cgctgcgttc ccggcggcct accggggcac cgtcagcgta
     7561 cgcattgcag ttagggacat tcagacgatt gagcagcttt cttctgcaca gccaatcacc
     7621 atgcgacttt atcggcccgt cgaagcggac agccaggccc tgcgcctgag ggtgtatcga
     7681 ctgggcgagc cggtaccgct ctccgggagt ctgccgatgc tcgagaacat gggggtgtcg
     7741 gtgctggacg aacaatccta cacgctcgac cgcggcgacg ccggcaaggt gcatatccat
     7801 gacttcggaa tgcgcaccgt cagcgaattg ccggacatct cgacggtgcg accgctgttc
     7861 gaggatggtt ttgccaaggt atggcggcgt gagatcgaaa gcgatggatt caatcgtctg
     7921 gtgctcgccg cagggctgtc gagcgatgaa gtcgttgtgc ttcgcgcctt tgccaaatac
     7981 cttcggcagg ccgggtttac gttcagtcag gcttacatcg agcagaccct ggtcgacaac
     8041 acccggctta ctcgcctgct ggtggccttg ttccatgtgc gtttcgaccc cgcggcaaga
     8101 ggagaccgtg atgccgccca gtcgaccctg gtcgaggaaa tcaaagaggc gctggatgaa
     8161 gtcgcaagtg ccgacgatga ccgcatcatc cggcgcttcc tcgccgtcat tcaggcgacg
     8221 atacgcacca atttctacca gcgcgacggg gctggatgcc ggaagccgta tgtctccttc
     8281 aagcttgagt cggccaaagt ccccgaactg cccgagccga agcctctgta cgagatctgg
     8341 ttctactcgc cacgcgtcga gggcatccat ttgcgcggcg gcaaggttgc ccgaggcggg
     8401 ctccgctggt ccgatcgaat ggaagatttc cgtaccgaga ttcttggact ggtgaaagcg
     8461 cagatggtca aaaacgtcgt catcgtgccg gtcgggtcaa aggggggatt cgtgctgaag
     8521 aatccaccgc cgcagacgga tcgcgaagcc tatctgaacg aaggcatcga gtgctacaag
     8581 tcctttcttc gtggcctgct cgacgtcacc gacaatctgg tggccaacga attggtgcct
     8641 ccgaaaaacg tagtccgaca tgacggcgat gacccctacc tcgttgtcgc ggctgacaag
     8701 ggcacggcca cgttctcgga ctatgccaac gctgtcagtg aagaatacgg gcattggctc
     8761 ggcgatgcat ttgcgtccgg cggctcggtc ggctacgacc acaaaaagat gggcatcact
     8821 gcgcggggcg catgggagtc tgtcaaacgt cacttcctcg aaatggggat cgacacgcag
     8881 acgacagcgt tcaccgtggc cggcgtaggc gatatgtcgg gcgacgtttt cggcaatggc
     8941 atgctgttgt cacggcatat caaactgctg gcggcattcg accatcgaca catttttctg
     9001 gacccgaatc ccgataccga ggtgagcttc aatgagcgcc agcgcttgtt cgtactgccg
     9061 cgttcatcct gggacgatta cgacaaatcg ctgatatcgc atggcggcgg ggtttacccg
     9121 cgcagcgcaa agtcgattcc cctgaccgag gaagtgcgac agatacttgg cgtgtccgcc
     9181 gatgcgctga cccccgtcga gctgatgcgg gcgattctca aggcgccggt cgatctgttt
     9241 tacaacggcg ggattggcac ttacatcaag tccagggaac aaagcaacgc cgaggtcggc
     9301 gatcgtgcca atgacgccgt gcgggtcaac ggggccgatc tgcgctgcaa ggttgttgcg
     9361 gaaggaggca atctcggctg cacgcaactg ggacgcatcg aatttgccat gaatggcggc
     9421 cgcatcaaca ccgacgcaat cgacaactcg gcgggagtgg actgttcgga ccacgaggtc
     9481 aacatcaaga ttctgctggg cctgatcgaa gcagacgggg agttgaccaa gaagcagcgc
     9541 aacaagcttc tggcggagat gaccgatgaa gtgggtgagc tggcgctggc cgacaactac
     9601 tatcagaccc agtcgcaatc ggtcacaggc acccgcgcgg tcaagatgct cgacggccac
     9661 cagcgcttca tccgccatct cgaaaaggcc ggacgtctcg atcgggcggt tgaatatctg
     9721 cctaccgacg aacagattgc cgagcgtcgc gcggcacggt ccgggctgac cgcgccggag
     9781 cgggcggtac ttctcgctta cagcaagatg gtgctattcg accaactgct tgagtccgat
     9841 ctggtggatg acccctacgt tgctccggct ctcacaggat acttccccaa ggtgctgcag
     9901 gcgcgatacg ccaacgcgat gcttcggcat cccctgaagc gggaaatcgt tgctacggtt
     9961 ctgaccaatt cgatgatcaa tcacgtcggt atcgttttcg tgtttcggat gtgcgacgaa
    10021 accggcgcgg aagcgcacga agtggttcgc gcctatattc tggcgcgcga catattcgac
    10081 ttcccggcgc tttgggctga aatcgaagcg cttgacggca aggtcgctgc tcctgtgcag
    10141 aaccggatgc tgattgatgc tggacgcctc gtattgcgga ccgtactgtg gttcttgcgc
    10201 acgcgtcacg gcaaactgcc attcagcgaa atcatcgagc ttttccggcc cggtgttgcc
    10261 cagctcggca gcaagctttc ggcatttctg aacgtcggag atttgaatgc gcttaacgaa
    10321 cgacgggcgg aactggaagc agccggtgtg cctgccggca ccgcagagca ggttgcgaag
    10381 ctggacccgt catacgccgc acttgatatc gtcgatattg cgacggacag cgagcaaggc
    10441 gtcgaccggg tcgcagagat ctattttgcc ttggtcggga agctggaaat gcgctggttt
    10501 ggcgatcaga tcaatgcgct ttccgccaat acgcactggc agggactggc gcgcaacgcc
    10561 ctgcgcgatg acctggcgcg gcagacgcga ctgctgaccg ccagcgtcat tcgcctgtcg
    10621 ccaggcggga ccgatgcagc ggaaatgctt gccgcctggg aagcttcaaa ccatgcgccc
    10681 ctgagccggc tgcgggaaat ggtggccgat ctgaagacgg gcccggcact ggatctggcg
    10741 atgctttccg tcgccatgcg cgaactgagg agtcttacct aatcaacaaa gtcgccctcg
    10801 caatttgtgc gttgcagaac aagcggcccg acagacttta ttccgctccg acaggcttta
    10861 tttattgcaa tcacctgctg cgctcggcca tccacgggct gggcggccgc ggcgcgcgac
    10921 cgcaaatcct gctgactacg gtgaaggacg aggagcatat gctcggcctg ctgatggtcg
    10981 aggcactgct cgccgccagc ggagcctggt gcctttcgct cggcgcccag acgccgattg
    11041 ccgatatcgt tggcgccgcg cggggcacca cgatcgacat cgtcgccgtc tcgtcttccg
    11101 gcgcctatcc ggctcgatcc gaaagtcagt ccgcgctcaa aaattacttg actgttttta
    11161 tcaagttaag taaattgcgc ttgtcccaag ggggagtagc tctctgccgc ggcgtcgtca
    11221 atacggaaat ccttgtcgtc gaagggtttc ccggcgcccc gggtgcttgt cgctagacag
    11281 gcgcgagcaa gacctttgcc gcgatggcgg aggttccctt gtcaaaaaca cggcccttcg
    11341 tcctcgctgg ataaagggcc gttccgtttg cagccagcgc tgccgacgga acggcaaacc
    11401 ggatctggca aggagagcat gcaatggaaa caatcggtac gtggtggatg tgggcgggat
    11461 tcctcgccat cgtcctggtg atgctggcga tcgacctgtt tctggtcggc ggcggcaagc
    11521 agcacaaggt gtccttcagg gaggccgcga cctggtcggt gatctgggtc tccgtatccc
    11581 tcctcttcgc cgcggcactt tggtggcatc tcgatggcag cgccgggcgc gaagtggcca
    11641 accagaagtc gctggaattc gtgacgggct acctgatcga gaaatcgctg gcggttgaca
    11701 acgtcttcgt ctggctgatg ctgttctcgt tcttcggcat cccgctggaa ttgcagaagc
    11761 gcgtgctggt gctcggcgtg ctcggcgcca tcgtattgcg cacggtgatg atcttcgccg
    11821 gcgcctggct gatcacccag ttccactgga tgctctacat cttcggcgcc ttcctgctgg
    11881 tcaccggcat caagatgtgg tggttcgccg aggaaaagcc cgacatggcg aacaacccgc
    11941 tgatccgctg gctgcgcggc cacatgaaga tcagcgacga actccacggc gagaagttct
    12001 tcgtctggaa ggacggcgtg cgctacgcga cgcccctgtt cctgaccctg atcctggtcg
    12061 agatctcgga cctgatcttc gccgtcgatt cgatcccggc catcttcgcc atcaccgcgg
    12121 atcccttcat tgtactcacc tccaacgtct tcgccatcct cggcctgcgc gcgatgtact
    12181 tcctgctggc cgacatggcc gaccgcttct cgctgctgaa gtacggcctg gcggcggtgc
    12241 tggtgttcat cggcgtcaag atgctgctga tcgacctcta caagatcccg atcggcttct
    12301 cgctggccgt ggtcgccacc ttcatcggca tctcgatctg gatgtcgctg cgcaaggaaa
    12361 agctggcgcg tgccgcggct gcaggccgcg cgcgcgagtg aggagccaac atggatatcg
    12421 taatgcacac tcctggagga tggcgccccc ggctggaccg ctggttggag tccgcgccga
    12481 tccataacag cctgatagcg ttgatcctga tcaatgccgt gatactcggc ctggaaacca
    12541 gcccggcgct gatggcgcag tggggctcct ggctgagtgc tgccgaccgg gcgatccttg
    12601 ccgttttctt ggtcgaaatc gcgctgcgcc tgctggccca tcgcctcgtc tacttccgcg
    12661 atccgtggaa cgttttcgat ttcacggtgg tggccattgc gctgattccc gcctcagggc
    12721 ctttggctgt gttgcgcgcg ctgcgcatcc tgcgtgtact gcgcctgatc accctggtgc
    12781 cgagcatgag gcgggtggtc ggcggcctgc tcgcggccct gcctgggctg ggctcggtgg
    12841 tggggatcat ctgcatcatc ttctatgtcg ccgccgtgat cgcgacgaaa ctgttcgccg
    12901 cagactttcc cgcgttgttc ggcgacctcg gcagcagcgc cttcacgctg ttccaggtga
    12961 tgaccctcga gggctgggcg atggaggtgg tgcgcccggt gatggccgtt tatccttcgt
    13021 cctggatctt cttcctgatc ttcatcctgg cctccacctt caccttgctc aacctgttca
    13081 tcgcggtcat cgtgaatgcg atccaccagg agggcgccga ggaccgccgc gacacgaccg
    13141 cggccgaaat cgcagggcta cggaatgaaa ttgcagcctt gcgcagtcat cttggaccca
    13201 tgcgcaggtc ctcgccctat gcaacacgga ggcgctgaat gatggaacca acgcgactgg
    13261 cggattggga tcacgccggc ctggcgctgg cccatgcggc acgcagcccg tggcttgaca
    13321 gcctgttcgc caccgtcacc tggctcggtt cactggcggt actagtgctg ctggcgctgc
    13381 tgctcgggtg gcggcggtgc ggccacagga acgcggcatt tgtcacgctg gcgctgatcg
    13441 gtgcctccgc gctcgggcac ttggtcaagc tgatcgcggc gcggccgcgg ccggatctgt
    13501 ttccgccgct gatcccgatg ccggacgatg ccagtttccc aagtgcccat gccatgcagg
    13561 tcaccgcctt cgcactggcc tggctgctgc ggccgggagc atcgccgggc cggatcgaaa
    13621 ttgccgtcct gctcgctgca gcgtctctgg tcggcgcctc acggctctat ctgcaagtcc
    13681 actttccgag cgatgtcgtc gccggcgtaa tgcttgcgac cctttgggtc gttctcctgc
    13741 gtcgcctgcc ggcatggcgg gagaatagct aatgcgacac aagttcctcg gcaccggcga
    13801 cgccggttac cacccgctgc gcaagatccg gaccgccatt tcggggctgc gctacgccgt
    13861 gctctacgac tggtcggtca cttacaaact gatcgcgtcg gtggtggtgc tggcggtcgc
    13921 cttcgccgcg cgcgcctggg tggatttcct gctgatcctc gtcgtgaccg gattcgtgct
    13981 ggtcgccgaa atcttcaaca gcgcgatcga agccctctgc gattttgtcg agacgcgcca
    14041 caacgagaag atcaaggtca ttaaggacat cgccgccgcc ggcgttggca tcgcgatctt
    14101 cgtctggttc attgtgctcg gcgtcgagct gggccgcttc ctcgacgcgc ctttaccgtg
    14161 gatgcgcctg tagtctcgtg gttggacatg cctcgtcgat ggcgcgggga acagaccacg
    14221 gtagtcgcga accacgggcc gcatcgctcc ttggtccggc aagcctccgg gcaatagact
    14281 caatataatt cagattaatt gttgggagca ctttgatgaa gatcgccgta ttcagcgcca
    14341 agcgctatga ccgggaattc ctcgatgccg cgaatgccgc caacgccggc gccggccatc
    14401 aagtatcgag tcacgctgac ggatgatgag gttgaaatgc tggaagcctt gctgcgcaag
    14461 ggaaggagcg cggcgcgcaa gcaaacgcga gcacggattt tgttgaaagc ggcagccggc
    14521 ggcaaggacg ccgaaatcat ggaagccctg gcagtttctg ccaccatgat ctacaacacc
    14581 cggcaaagag gtgtcgagga aggcgttgag gcggccttgc acgaccggcc gcgcccgggg
    14641 aagacgccta aattgacgga caagcagtgt gcacaagcgg cgcaccaaga tcgacttcgc
    14701 ccactgtatg cggcatatcg tccagaccta cccggatgcc gaggtgattc gcgtcgtgct
    14761 cgacaacctg aatacgcata aacccgcctc gttctacgaa gccttcccgc ccgacgaggc
    14821 ccgcgccatc gccaagaggc ttgagttcca ttacacccct aagcatggca gttggctcaa
    14881 tatcgcagaa atcgagttgg ctgt
//
LOCUS       DAORPM010000022        14518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_827_length_14518_cov_10.227754, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000022 DAORPM010000000
VERSION     DAORPM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 14518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14518
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_827_length_14518_cov_10.227754"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1137
                     /locus_tag="PLD53_02095"
     CDS             199..1137
                     /locus_tag="PLD53_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRC59726.1"
                     /translation="MDTFLHHVGLAAPLFALVFVGYALMRFSGWPTAMSKNLSEFVFS
                     VALPALLFRLMSDFSKLPPVDARLLIAFFGGCLIVFVIGRLIAWKLFALDGVSQSVFA
                     LGGVFSNNVLLGLPLAKVALGDAAVPSVALVLVFNALILWTLVTVSVEWARHGSFSAH
                     GFAKTLRGVLTNPIVAAILSGALFGLNGWHLPAAIDVPLSMVGQAATPMALIALGMGL
                     AEYGVREGWKISVAICAVKLLIQPLVVWALARLLGLPVMETRVVVLLASIAVGVNVYL
                     MSRQFKVLEGPVASSLVLSTGLAALTTPLALTLTGL"
     gene            complement(1147..1791)
                     /gene="folE"
                     /locus_tag="PLD53_02100"
     CDS             complement(1147..1791)
                     /gene="folE"
                     /locus_tag="PLD53_02100"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRC59727.1"
                     /translation="MTDNSDHEHTPPLSCEIGIPRPNEFDPAAFEQAVSDLLKACGIA
                     PDITHMGRTAQRVRELWQQRLLGGYDLDPASALGTGFDDHRRDMVIVRGIAVHGVCPH
                     HLVPFRGLAHVAYLPGGRLHGFGRIARMVDAIGHRFTYQEWMTRDIAEALMHHGKARG
                     AACVVEAEQLCLLLGEDRRGDERVITQCFTGEFETSSQYRNEFLRAVGSVRRSD"
     gene            2002..3246
                     /locus_tag="PLD53_02105"
     CDS             2002..3246
                     /locus_tag="PLD53_02105"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRC59728.1"
                     /translation="MKRVDDFRLRFGKKELVPIVIGGMGVDISTAELALEAARLGGVG
                     HISDAMVNTVADRRFNAKFVKDKLKQYKFNVANTDKSMVKFDLDQLVEATKMHVGRTM
                     EAKRGDGLIFVNCMEKLTMNAPRETLRVRMHAALDAGIDGITLAAGLHLGSFALIEDH
                     PRFRDAKLGIIVSSLRALQLFLRKTARTNRLPDYVVVEGPLAGGHLGFGMDWADYDLS
                     TIVTEIQRFLLAEKLDIPLIPAGGVFTGSDAVAFLEKGAAAVQVATRFTVARECGLPE
                     DVQQKYFLASEDQIEVNQISPTGYPMRMLSNSPAIGDGIRPNCEAYGYLLDSNGCCSY
                     ITAYNREVEANPGVKRLSVKDKTCLCTQMRNFDLWTCGHNTYRLKDTTRKLDDGSYQL
                     LSAEHIFRDYQFSTDNVIALPE"
     gene            3326..3703
                     /locus_tag="PLD53_02110"
     CDS             3326..3703
                     /locus_tag="PLD53_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRC59729.1"
                     /translation="MGTKLYVGNMSYGIDKNDLEQMFAAHGTVASAQVITDRETQRSK
                     GFGFVEMGSDQEAQAAIAALNGKSIDGRALTVNEARPQEPRSSGGFGGPRRGPGGGGG
                     RGPHGSGRGGSGSGSSGGGGPRY"
     gene            3775..3993
                     /locus_tag="PLD53_02115"
     CDS             3775..3993
                     /locus_tag="PLD53_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59730.1"
                     /translation="MLHSVFVPIRDIVLAKPNYSFEKRQRDLEKKRKQEEKRQRKLAD
                     KKPAGDDGGQSSPEDGAPPDADPAGTGA"
     gene            4138..4983
                     /gene="rlmJ"
                     /locus_tag="PLD53_02120"
     CDS             4138..4983
                     /gene="rlmJ"
                     /locus_tag="PLD53_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRC59731.1"
                     /translation="MLSYRHAFHAGNHADVLKHFLLVQLTRYLGQKDKSFWYIDTHAG
                     AGAYALDSGYAAKLAEYKDGIGRLWARNDLPAALADYVELVRKFNPDRRLRAYPGSPY
                     FALWTMREQDRLRLFELHSKDARLLQERFLDAGKQVIVETTDGFAGLKALLPPPPRRA
                     VVLIDPAYEDKHDYERVFHALKESLTRFPDGTYALWYPQLTRLDAHELPQRLKRLPAK
                     TWLHVALRVKAPAKDGFGMHGSGMFILNQPWILRDTLAEVMPYLVQLLAQDAGAGFAL
                     EQSEA"
     gene            complement(5028..5384)
                     /locus_tag="PLD53_02125"
     CDS             complement(5028..5384)
                     /locus_tag="PLD53_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRC59732.1"
                     /translation="MSLQSLAQALLQFRDERNWAQFHTLRNLIVSLNLEAAELLELTQ
                     WKSDEEMVALSSRPDTQEALRDECADVLLYLLLIAEKAGIDLEEAAHAKLAKNAAKYP
                     VEKSYGSSRKYSRFEE"
     gene            complement(5411..6421)
                     /gene="mltB"
                     /locus_tag="PLD53_02130"
     CDS             complement(5411..6421)
                     /gene="mltB"
                     /locus_tag="PLD53_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HRC59733.1"
                     /translation="MRKTFTTLLLVLLAGLMPLQSHAAKKNAPRTPPFSEAPEVRVFI
                     LDMHERHGFDVDHLTRQFGAIHSNAIVLRAIRPAAVPEMQRSWLRYRQRFVNERRISN
                     GLRFWQENGAELARAEAIYGVPPEIITAIIGVETEYGRNMGKFSVLEALATLAFDYPP
                     RAEFFRNELEQFLLMARENGVSPLEIKGSYAGAIGIPQFMPSSQRHYAVDFDGDDRID
                     LRQSTTDSIGSVARFLNMHGWQAKAPIAVPATVEGDPTALINAGIKPVLPLKDLLEHG
                     VTARGDDERPAALIDLVSPYQATEYWIGFENFYVITRYNRSSFYAMSVFQLAESLRKS
                     KE"
     gene            complement(6418..8421)
                     /locus_tag="PLD53_02135"
     CDS             complement(6418..8421)
                     /locus_tag="PLD53_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HRC59734.1"
                     /translation="MASRPAKPPPSLEHAAIPWLLAVAFATAAPHAAHLPLWITLLVG
                     SAFLWRGWLWQQNGRMPPRWLLALLVFFGSAGIAWQFHTLFGRDAGVALLVFFMALKP
                     MEMRTRRDAMVVIMLGFFLLLTHYFYSQSIPTGIWLLASTTLLTAALIRLHGGPQPVG
                     AILRYAGLLLAQAIPFMLIVFLLFPRISGPLWGLPQDAYSGLTGLSDKMSPGSLNNLI
                     QSGAIAFRVQFSGDPPKQSQLYWRGPVLNDYDGQTWRGQTNTLQRPSQTPVIEARGKT
                     YSYVTTLEPHNQRWLLALDLPVSLPDGSAFSPTFETRIREPLVSRGRYAFTSTVDFVV
                     NRQETPGLLKRALHLPPGFNPLSRELANEWRSEFKSPEQISIAALRFFRQEAFFYTLQ
                     PPLLGEHAVDEFLFTTRRGFCEHFASAYVFLMRAAGVPSRVVTGYQGGEINPVDGYLT
                     VRQSDAHAWAEIWLAEQGWVRVDPTAAIAPSRIEQGINAALPSGDPLPALVRIDTNWL
                     RTLRNRWEAANNAWNQWVLGYNPERQRDVLSRLGLSEPDWRNMTAALATLCGITLLIV
                     TLWTLKRRKVLTPAQCAWQRFCFKLKRLGIVRADWEGPLAFAARVAREHPEFGVLTSK
                     AAGHFADLHYGTGRPEQLQQLKQCTRSLPHLAKRQPSSRRNRN"
     gene            complement(8414..9364)
                     /locus_tag="PLD53_02140"
     CDS             complement(8414..9364)
                     /locus_tag="PLD53_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRC59735.1"
                     /translation="MPLIQQLRQWLFRFGRDEHLPIVLTQRRIFILPTRTGVLFGAVL
                     CIMLIGAINYNLSLGYALVFLLAGLGIVAMVHTFRNLVALRLTPGRVEPVFSGEIARF
                     HMILENEHPQARRALDLAFGHNESVSVDILPGAQAMIAIPCPAPTRGRLDPGRITLST
                     RYPLGLFCAWSYPHPRISCLVYPNPIETPLPPPSAVSHTGQRHGDNGQEDFTGLRVRQ
                     LNDSPRHIAWKAVARDIENRPLLIKQFAGGAAQELWLDLSLAPPNSGPEMKLSILTGW
                     VIAAERTQTRYGLRLPNLELAPDLGNNHRNACLEALALYG"
     gene            complement(9364..10293)
                     /locus_tag="PLD53_02145"
     CDS             complement(9364..10293)
                     /locus_tag="PLD53_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRC59736.1"
                     /translation="MPSIEKKSSIVDVIAAANQIILGKNEQIRLALACLLARGHLLIE
                     DLPGVGKTTLAHTLARLLGLQFSRIQFTSDMLPADVIGISIFDRESSNFKFLPGALFS
                     HVLLADEINRATPKTQSALLEAMEERQVTTEGETRPLPDPFFVIATQNPSSQIGTFPL
                     PESQLDRFLLRIELGYPDRQAERALLEGGGRREHLPELAACFNPDQLIPLQKQAADIH
                     VSPALLDYVQLLIEATRNSPMFVHGLSPRAALALIAVARAWTFIDGRSMVLPEDIQAV
                     LPSVACHRLRLVHSADHARPEDIANLIRAIPLV"
     gene            10414..11340
                     /locus_tag="PLD53_02150"
     CDS             10414..11340
                     /locus_tag="PLD53_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRC59737.1"
                     /translation="MNSTAFITHHDCHLHDMGSYHPESPARLSAISDQMIAQGLDHYF
                     AHYEARLATFEQLLRVHSISHLEHLKRSSPALGVVHLDPDTAMNPHSFQAALRSAGAG
                     VMAVDLIMADEVKNAFCAVRPPGHHAERSTAMGFCFFNNIAVAAAHALKAHGLERVAI
                     VDFDVHHGNGTEASFQNNEQVLMVSTFQHPFYPYSGIDNPAPNMVNVPLPWGADGEQF
                     QQVVNDIWLPRLRAFKPEMIFISAGFDAHYEDDLGGLKLLEKDYTWVTEQVKQIADET
                     AQGRIVSMLEGGYVLSALARSVVAHIKVLTGI"
     gene            11454..12338
                     /gene="dapA"
                     /locus_tag="PLD53_02155"
     CDS             11454..12338
                     /gene="dapA"
                     /locus_tag="PLD53_02155"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRC59738.1"
                     /translation="MNTFTGSMVAIVTPMHEDGSLDLDGMRRLVDFHVQEGTDAIVVV
                     GTTGESPTVDVEEHRELIQLVVEYAAGRIPVIAGTGANSTAEAIEMTHFAKKAGANAA
                     LSVVPYYNRPTQEGLYRHFKAIAEAVDIPLILYNVPGRTVADMSSETSLRLALIPNIV
                     GIKDATGNIDRGSELIARAPKGFAVYSGDDSSACALMLLGAQGNISVVANIAPRLMHE
                     MCAAALKGDLLKARELNFRLLGLNRQLFCEANPIPVKWACQELGLIKEGIRLPLTPLA
                     PEFHERVRTAMRQAGLLA"
     gene            12362..13501
                     /gene="bamC"
                     /locus_tag="PLD53_02160"
     CDS             12362..13501
                     /gene="bamC"
                     /locus_tag="PLD53_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HRC59739.1"
                     /translation="MRFAVFRLSFCALAVVLSSCSGIGLESKKIDYKTSAATKVPTLE
                     IPPDLTSPTRDDRFAVPDTAGKGTATFSAYSGERSPQALAQQQSGVLPQVDKARIERS
                     GNQRWLVVAGTPGKLWDQIKDFWQETGFVIKLELPEAGVMETDWAENRAKIKEDALRN
                     LLGKVIDSLYSTAERDKFRTRLEPGLEPGTTDVFISHRGMYEIYVTEGKDQTKWQPRE
                     PDPELEAEMLRRLMVRLGSDDKRAAAQIAAAKEKPADRARLSRGNDGAGTLEVQESFD
                     RAWRRVGLALDRVGFTVEDRDRSKGLYFVRYVDPEVDGQKKDDGFMSKLAFWKASGPD
                     PKIKYRIYLKEVGSSTTVQVLSFEGGVDQSETSKKILSLLYDQLK"
     gene            13501..14265
                     /locus_tag="PLD53_02165"
     CDS             13501..14265
                     /locus_tag="PLD53_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC59740.1"
                     /translation="MRFASLGSGSRGNSLLVDAGDTKLLLDCGFSVRATTDKLGRLGV
                     SPEEISGILVTHEHGDHIAGVFKFASRFSTPVYLTHGTHIAAPSGKSLSTCRMIDSHA
                     SFTIGNLEIHPFPVPHDAREPVQFAFSDGAHRLGVLTDTGSITQHVIDVLRVCDALVL
                     ECNHDSELLAASSYPMTLQRRISGNFGHLSNRQAVSLLLQIETRQLQHIVAAHLSEQN
                     NRHELVVAALATALNCADDWIGVAGQEEGFGWRQLV"
BASE COUNT         3001 a         3847 c         4472 g         3198 t
ORIGIN      
        1 ggtggtcaat gaagcgggtg cggaagatgg accggaaacg accgggcacg cggggtgcgt
       61 ggcgaaagct tgatgcttca cagcgtattg tcgcgcgaat tctacagtcc aagttcagta
      121 catgctcctt cgaatccatt aaacttcggg cctgctcagc gagccaggcc tttgttcacg
      181 tttgaagact gttcttttat ggatactttc ctgcatcacg tagggcttgc cgctcccctg
      241 tttgctctgg tttttgtcgg ctatgcgctg atgcgttttt ccggctggcc gacggcgatg
      301 tcgaaaaacc tgtcggaatt cgtcttctcg gtagcgctac cggccttgct gtttcgtctg
      361 atgagtgatt tctccaagct gccgcctgtc gacgcgcgct tgttgattgc cttcttcggc
      421 ggctgcctga tcgtatttgt catcggtcgc ttgatcgcgt ggaagttgtt tgcgctcgac
      481 ggggtatcgc agtcggtatt tgcactgggt ggcgtgttct ccaacaatgt ccttcttgga
      541 ttgccgctcg cgaaagtggc ccttggcgat gcggcggtcc cgtcggtcgc actggtgctg
      601 gtgttcaacg cgctgatact ttggaccttg gtgacggttt ctgtcgaatg ggccaggcat
      661 ggcagttttt cggcgcacgg atttgccaag accctgcgcg gcgtactgac caatccgatc
      721 gtcgcagcca tcctgtccgg tgcactgttc ggtttgaacg gttggcactt gcctgcggcc
      781 attgatgttc cgctgagcat ggtcggtcaa gcggcgacgc cgatggcgct gattgctctt
      841 ggcatgggtc tggccgaata cggcgtacgc gagggctgga aaatcagcgt cgcgatttgc
      901 gctgttaagc tgctcattca accgctggta gtctgggcgc tggccaggct gcttggactc
      961 cctgtgatgg agacacgggt ggtggtattg cttgcttcga tcgccgttgg tgtcaatgtc
     1021 tatctcatgt ccagacaatt caaggtgttg gaagggccgg ttgccagcag cttggtatta
     1081 tcgaccggac tggcggcctt gacgacgcca ttggcgttga cgctaaccgg tttgtgaacc
     1141 ctgcgttcaa tcgctccgcc gaacgctgcc aacagcccgc aggaactcgt ttcggtattg
     1201 gctggacgtt tcgaattcgc cggtaaagca ttgggtgatg acgcgttcgt ctccccgccg
     1261 gtcttcgccg agcagaagac acaattgttc ggcttcaacc acgcaggctg ctccgcgtgc
     1321 cttgccgtga tgcatcagcg cttcggcaat gtcgcgggtc atccactcct gataggtaaa
     1381 gcggtgcccg atggcgtcaa ccattcgggc gatgcggcca aagccgtgca ggcgacctcc
     1441 gggaaggtag gcgacatggg cgagtccgcg aaagggcacc agatggtgcg ggcagacgcc
     1501 gtgaacggct atgccgcgga caatcaccat atcgcggcga tggtcgtcga atccagtacc
     1561 cagtgcgctg gccggatcaa ggtcataacc accaagcaga cgctgttgcc agagttcgcg
     1621 cacccgttgg gcggttcttc ccatatgcgt gatatcgggc gcaatgccac aggctttgag
     1681 caggtcgcta acggcctgct caaaggcagc ggggtcgaac tcattcgggc gtggaatgcc
     1741 aatttcgcaa gacaaaggtg gagtgtgctc gtgatcgctg ttatcggtca tgacgtgttc
     1801 ccgtttcgaa gtgctgatgc ggacgatgat acacgcctcg ggtgtgcaaa tttgaacccg
     1861 accgcaggtg tcattgctac ccttttcgta gagcgagata cgtaattcag tgcctggatg
     1921 ggcgataata cggaacctgc gaggatttta ggtctcgaca tcgcagtgtg tgcagcctgg
     1981 tcgggttctg gaaaggtata gatgaagcgt gttgatgatt tccgtttgag gtttggcaag
     2041 aaggagttgg tgccgatcgt gatcggtggt atgggggtgg atatctcgac agccgaactg
     2101 gcgcttgagg cagcccggct gggtggtgtg ggacatattt ccgacgccat ggtcaatacg
     2161 gtggccgacc ggcgtttcaa cgccaagttt gtcaaggaca agctcaaaca gtacaaattc
     2221 aacgtggcca acaccgacaa gtcgatggtc aagttcgatc tcgatcagct tgtggaagcg
     2281 acaaaaatgc atgtcggcag gaccatggaa gccaagcgcg gcgatggtct catcttcgtt
     2341 aactgcatgg agaagttgac catgaacgcg ccgagggaga ccttgcgtgt tcgtatgcat
     2401 gcggcacttg atgccggtat cgacgggatt acactggctg ccggtttaca tctcggatcg
     2461 tttgcgctga tcgaggatca cccgcgcttt cgcgacgcaa aactgggaat catcgtttca
     2521 tcgctacggg cgttgcaact cttcctgcgc aagacggcgc gcaccaaccg cctgcccgac
     2581 tacgtagttg tcgagggccc cctggctggc ggccacctgg gtttcgggat ggattgggcc
     2641 gactacgatc tgtcaaccat cgtgactgaa atccagcgat tcttgctggc tgagaaactt
     2701 gacattccac tgattccggc cggtggcgtt tttaccggaa gcgatgctgt tgcctttctt
     2761 gagaaaggcg ccgcggccgt acaggtggca acacgtttta ccgtggctcg agaatgcggt
     2821 ttgccggagg acgtgcagca gaagtacttc ctcgccagcg aggatcagat cgaggtcaat
     2881 cagatatcgc cgacgggata cccgatgcgc atgttgtcca acagcccggc gattggcgat
     2941 ggcatacgac ccaactgcga ggcttatggc tatttgctgg actccaacgg ttgttgttcc
     3001 tatatcaccg cctacaaccg cgaagtggag gcaaatccgg gggtcaagcg cctttcggtg
     3061 aaagacaaga cctgtctgtg cacgcagatg cgcaacttcg atctctggac ctgtggccat
     3121 aacacgtatc gcctcaagga tacgacgcgc aagctcgacg acggcagcta ccagttgcta
     3181 agtgcagagc atatcttccg tgattatcag ttcagcacgg acaacgtgat cgctttgcct
     3241 gaataaatat agttagactc cggggtccgg ccttgctatt gttccggagc agtctttgca
     3301 acgtctcatc ctcggagcat cttcattggg cacaaagcta tacgtaggca acatgagcta
     3361 cggcatcgac aagaatgatc tggagcagat gtttgccgcg cacggcacgg tcgcttcagc
     3421 gcaagtgatc accgatcgtg aaacgcagcg ctcgaagggt ttcggatttg tcgagatggg
     3481 ttcggatcag gaagctcagg cggcgattgc cgcactcaat ggcaaaagca ttgacggccg
     3541 ggcactgacg gtcaatgaag cgcgaccaca ggaaccacgc agtagtggcg gcttcggagg
     3601 tccacgtcgc ggtcctggcg gtggaggtgg gcgcggaccg cacggatcgg ggcgcggagg
     3661 ttcgggtagt ggcagttctg ggggtggcgg tcctcgctac tgattgattt gcagccacgg
     3721 gggcagtgaa cgccggttga tcttgacggc tgtgcttatc cggggctttt tgtagtgctt
     3781 cattcggtct ttgtgccgat cagggatatt gttttggcaa aaccaaatta ttcgtttgaa
     3841 aagcgccaga gagatctgga gaagaagaga aagcaggaag aaaaacgcca gcgcaaactt
     3901 gcggataaaa agcccgcagg tgacgacggt ggtcagtctt cgcctgaaga tggtgcaccg
     3961 ccggatgccg atccagccgg caccggggcc tgatcgacag gttccaggat attcgctgga
     4021 aatcgggagt tgctgaaaga gcggggcttg tctcgcctgc cggactcgtt ctccgcgtac
     4081 ggttgggacc aacatcctcc gcaggaatag tcgaataatc gcagaccgac ttagtccatg
     4141 ctcagctatc gtcacgcctt ccatgccggc aatcacgccg atgttctcaa gcactttttg
     4201 cttgtccaac tgacccgtta tctcggtcag aaagacaaat cattctggta catcgatacg
     4261 cacgcgggcg ccggtgctta tgctctggat tccggttatg cggccaagct cgccgagtac
     4321 aaggatggga tcggtcgatt gtgggcgcgc aatgacctgc cggcagcact ggccgactat
     4381 gtggaactgg tacgcaagtt caatccggat cgtcgcttga gagcctaccc cggctcgccg
     4441 tattttgccc tgtggacgat gcgtgagcag gatcgcttgc ggctctttga actgcacagc
     4501 aaggacgcgc gcctgttgca ggagaggttt ctggatgctg gcaaacaggt gatcgtcgag
     4561 acgaccgacg gctttgccgg actcaaggcg ctgttaccgc cgccacctcg tcgcgccgta
     4621 gttctgatcg atccagctta tgaagacaag cacgattacg agcgggtgtt tcatgcattg
     4681 aaagaatcat tgacgcgctt tcccgacgga acctatgcgc tgtggtatcc acagctcacc
     4741 cgcctcgatg cacatgaatt gccacagcgc ttgaagcgtc tgccggccaa gacctggctg
     4801 cacgtggcgc ttcgtgtaaa agcgcccgcc aaggacggct tcggcatgca cggcagcggc
     4861 atgtttatac taaaccagcc gtggattttg cgtgacacgc ttgccgaagt gatgccgtat
     4921 ctggttcaat tgcttgcgca ggatgcaggc gcaggctttg cgctggaaca gtctgaagct
     4981 tagccaccag ctccgggttc tggatagatc agccggcgtt caacgcttca ctcttcaaac
     5041 cgtgaatact tgcggctcga tccgtaactc ttttcaaccg ggtactttgc ggcgttcttc
     5101 gcgagcttgg cgtgtgccgc ttcttccaga tcgatgccag ctttctcggc gatcagcagc
     5161 agatagagca gcacatcggc acactcatca cgcagcgctt cctgagtgtc aggccggctt
     5221 gacaaggcga ccatttcctc atcgcttttc cattgcgtga gttcgagcaa ctccgccgct
     5281 tccaggttga gcgagacaat cagatttcgc agcgtgtgaa actgcgccca gttgcgttcg
     5341 tcacgaaatt gcagcaaggc ctgcgccaat gactgaaggc tcatgaggtg ctgcccggtt
     5401 tattcacgct tcactccttc gattttcgca agctttctgc caactggaaa accgacatgg
     5461 catagaagct cgatcggttg tagcgtgtaa tcacgtagaa attctcaaag ccgatccaat
     5521 attcggtggc ctggtatggg ctgacaagat cgatcagtgc agcggggcgc tcatcgtctc
     5581 cgcgggcagt aacgccgtgc tccagcagat ccttcaatgg caaaaccggt ttgattccgg
     5641 cgttgatgag agctgtcggg tcgccttcca cagtagctgg gacggcaata ggtgccttgg
     5701 cttgccagcc gtgcatgttc aaaaaccggg caacactgcc gatggagtcg gtggtgcttt
     5761 ggcgcaggtc gattctgtcg tcgccgtcaa aatccacagc gtagtggcgc tgactgctcg
     5821 gcatgaactg ggggatgccg atggcgccag cgtaggagcc cttgatttca agtgggctga
     5881 caccgttttc tctggccatc agcaggaact gttcgagctc attgcggaag aattcggctc
     5941 gcggcgggta atcgaaggca agcgtagcca aggcttcaag aacgctgaat ttgcccatat
     6001 tccgcccgta ctccgtttca acgccgatga tcgccgtgat gatttcagga gggacgccat
     6061 agattgcttc ggcgcgtgcc agttctgccc cgttttcctg ccagaagcga aggccgttac
     6121 tgatccggcg ctcgttaaca aagcgctgcc gatagcgcag ccatgaacgt tgcatctcgg
     6181 gcacagcggc cggccggatg gcgcgcaaca caattgcatt ggaatggatg gcgccgaact
     6241 ggcgtgtcag gtggtcgacg tcgaacccat ggcgttcatg catgtcgagg atgaaaacac
     6301 ggacttcggg cgcctcgctg aaagggggcg ttcgtggcgc attttttttc gcggcgtgac
     6361 tttgcagtgg cattagtcct gccagcagga ccaagagcag ggtggtgaag gttttcctca
     6421 attcctgttt ctccttgaag acggctgccg cttcgccaga tggggcagac tccgcgtgca
     6481 ttgtttgagt tgttgtagtt gttcaggcct gccagtgccg tagtgcaggt cggcaaaatg
     6541 gccggccgcc ttgctcgtca ggacaccgaa ttccgggtgt tcgcgtgcta ctcgcgcggc
     6601 aaaagcaagt ggaccttccc agtctgcgcg gacgattccc agacgtttga gcttgaagca
     6661 gaagcgctgc caggcgcact gtgcgggcgt caaaaccttg cgccgtttga gcgtccacaa
     6721 ggtgacaatc agtaaggtga ttccacacag cgtggccagc gcggccgtca tgttgcgcca
     6781 gtccggctcg ctgagtccga gcctggatag cacgtctcgc tgtcgttccg gattatatcc
     6841 gagcacccac tggttccagg cattgtttgc cgcttcccag cggttacgca gggtacgcag
     6901 ccagttggtg tcgatgcgca ccagcgcggg caagggatct ccggagggga gcgcggcatt
     6961 gataccctgt tcgatccgtg agggcgcaat ggctgccgtg ggatcgactc gaacccaacc
     7021 ttgttcggcc agccagattt cggcccaggc gtgggcgtca gactgacgca cggtgaggta
     7081 accgtcgacc ggattgattt ctccgccctg atagccggta accacgcgcg acggaacacc
     7141 tgccgcgcgc atgaggaaga cgtaggccga ggcaaagtgt tcgcagaatc cgcgccgtgt
     7201 ggtgaagagg aattcgtcga cggcgtgctc gccaagcaag gggggttgaa gcgtataaaa
     7261 gaaggcttcc tgccggaaaa agcgcagtgc cgctattgaa atctgttccg gtgatttgaa
     7321 ttccgagcgc cactcgttag ctaattcacg ggaaagcgga ttgaatccgg gcggcaggtg
     7381 cagcgcccgt ttcaacaagc ctggagtttc ctgacggttg acaacgaagt cgactgtcga
     7441 cgtgaaggca tagcgccccc ggctgacgag cggttctctg atacgggttt caaacgtggg
     7501 cgaaaaggca cttccatccg gcagcgacac tggcaggtcg agcgccagaa gccagcgctg
     7561 attgtgcggt tcgagcgtcg ttacgtagct gtaggtcttg cccctggctt cgatgacggg
     7621 cgtctggctc ggccgctgca gggtgtttgt ctggccgcgc caggtttgac cgtcgtagtc
     7681 gttgagcacg gggccgcgcc agtagagttg tgactgcttt ggcggatcgc cagaaaactg
     7741 gacccggaaa gcgattgcgc cggactggat cagattgttg agcgaccccg gcgacatctt
     7801 gtcagaaagc ccggtcagcc cggaataagc gtcctgaggc agcccccaga gtgggccgga
     7861 gattcgcgga aaaagcagga agacaatcag catgaaaggg atggcctgcg ccagaagtag
     7921 accggcgtag cgcagaatgg cgcccacggg ttgagggccg ccgtgcaggc ggatcagtgc
     7981 ggcggtgagc agggtagtgg aggcgagcag ccaaatcccg gttggaatgc tctgcgaata
     8041 gaagtaatgg gtgagcagca ggaagaagcc gagcatgatg acgaccatgg cgtcgcgtcg
     8101 cgttctcatt tccatgggtt tcagcgccat gaagaagacg agcagggcga ccccggcgtc
     8161 ccggccaaac agggtgtgaa attgccaggc aattcccgca cttccgaaaa agaccagaag
     8221 cgccagcaac cagcgggggg gcatacggcc gttctgttgc caaagccagc cgcgccagag
     8281 gaatgcgctg ccgaccagca gcgtaatcca gagaggaaga tgggccgcat gtggcgctgc
     8341 tgttgcgaac gcaacagcca gcagccacgg gatcgcggcg tgttcgaggg atgggggagg
     8401 tttggcggga cggctagcca tacaaggcca aggcttcaag gcacgcgttg cggtggttgt
     8461 tgcccagatc cggagcgagt tcgagattgg gcaacctcag cccataacgg gtttgtgttc
     8521 gctcggcggc gatgacccaa ccggtgagta tggacagctt catttccgga ccgctgttag
     8581 gcggggctaa cgacaggtcg agccacagtt cttgcgcggc accgccagcg aactgcttga
     8641 tcagcagcgg gcggttttcg atgtcgcgcg ccacggcttt ccaggcgatg tgtcgcggcg
     8701 agtcgttcaa ctgtcgcacg cgcaggccgg tgaaatcctc ctggccatta tcaccatggc
     8761 gctgtccggt gtgcgagacg gcggaggggg gcggtagcgg ggtctcgatc gggttcgggt
     8821 agaccaggca actgatccgt gggtgcgggt agctccaggc gcaaaacagt ccgagaggat
     8881 agcgcgtcga gagcgtgatc cggccagggt caaggcgtcc gcgagtcggc gcggggcagg
     8941 ggatggcgat catggcttgc gcaccgggca gaatatcgac gctgacgctt tcgttgtggc
     9001 caaaggcgag atccagcgca cgacgagcct gaggatgttc gttttcaaga atcatgtgga
     9061 agcgtgcgat ttctccggaa aagacgggtt ctacccggcc tggggtgagg cgaagcgcaa
     9121 ccagattgcg gaaggtatgg accattgcaa cgatacccag gccggccagc aggaagacca
     9181 gcgcatagcc gaggctcagg ttgtaattga tggcgccgat cagcatgatg caaagcacag
     9241 cgccgaacag cactccggta cgcgtcggca ggatgaagat tcgacgctga gtcagaacga
     9301 tgggaaggtg ctcatcgcga ccaaatcgga acagccattg ccggagctgc tgaataaggg
     9361 gcatcagacc aggggtatgg cgcgaatcag gttggcgata tcctcgggac gggcatgatc
     9421 ggcactatgt accagtcgca gccggtgaca ggcgacgctg ggcagcaccg cctgtatatc
     9481 ctcaggcaga accatgctcc ggccatcgat gaatgtccag gcgcgagcga cggcaatcag
     9541 tgcgagggca gcacgcggac tcagtccatg gacgaacatc gggctgttgc gcgtggcttc
     9601 gatcagcaat tgaacgtagt cgagaagggc tggtgagaca tggatgtccg cagcctgttt
     9661 ttgcaatggg atgagctgat cggggttgaa acaggctgca agctcgggca ggtgctcccg
     9721 tcgtcctccg ccctcaagca acgcccgttc agcctggcgg tcgggataac cgagttcgat
     9781 gcgcaacaga aaacggtcaa gttgtgattc gggcaatgga aaggtgccga tctggctgga
     9841 tggattctgt gtcgcaatga cgaaaaaagg atcgggcaac ggtcgcgttt cgccctcggt
     9901 agtcacctgc ctttcttcca tcgcttcgag cagggcgctc tgcgttttcg gtgtggcacg
     9961 attgatctcg tcggcaagca atacgtgcga gaacaaggcg ccggggagaa atttgaagtt
    10021 gctgctttca cggtcgaaaa tcgagatgcc gataacgtcg gctggcaaca tgtcgctggt
    10081 aaactgaatc cgcgaaaact gcaaaccgag caggcgggcc aaggtatgcg ccagcgttgt
    10141 ttttcccaca cctggcaagt cttcgatcag caagtggccg cgtgcaagca ggcaagccaa
    10201 ggcgagacgt atctgctcgt tcttgccaag gatgatctga ttggcggcag cgatgacgtc
    10261 gacgatgctt gatttctttt caatggatgg catggcggat tgtaatattg tcgtaatggg
    10321 gcgattgggc tgataatggc ggtctggcct aaatgagtat ttctaccgat tctatcgcta
    10381 tatcccgtct tgatggacgg cagggaggca acattgaaca gcactgcatt cattacacac
    10441 catgactgtc atctgcatga tatgggttcc taccatccgg agagtccggc aagactgagc
    10501 gcaatcagtg accagatgat cgcgcagggc cttgatcact attttgctca ctacgaagct
    10561 cgcctggcga ccttcgagca actgttgcga gtgcattcga tctctcacct cgagcatttg
    10621 aagcgtagtt ctccggcact gggtgttgta caccttgacc cggatacggc aatgaatccg
    10681 catagttttc aggcggcatt gaggtcggcg ggtgcaggtg tcatggcagt tgatctgatc
    10741 atggcagacg aagtcaagaa tgccttctgt gctgttcgcc ctcccggaca ccatgccgaa
    10801 cgctcgacgg cgatgggctt ctgttttttc aacaacattg ctgtggcagc ggctcacgca
    10861 ctcaaagcgc acgggctgga acgggtggcg atcgtcgatt tcgacgtgca tcacggcaac
    10921 ggaaccgagg ccagcttcca gaataatgaa caggtattga tggtcagcac attccagcac
    10981 ccgttctatc catacagtgg aattgacaat cctgcgccaa acatggtcaa cgtcccactc
    11041 ccctggggcg ccgacggcga gcaattccag caggtggtca acgatatctg gctgccgcgc
    11101 ctgcgtgcat tcaaaccaga gatgattttc atctctgcgg gtttcgatgc gcactacgaa
    11161 gacgatctgg gtgggttgaa actgttggag aaggattaca cctgggtcac cgaacaggtc
    11221 aagcagattg ccgacgaaac ggctcagggg agaatcgttt caatgcttga aggtggatat
    11281 gttctatcgg cgctggcgcg cagcgtggtt gcgcatatca aggttctaac gggaatctaa
    11341 gcagattttc cggagcgttt gcgtcatccg attggcaacg accgcgtttt ccgtagttgt
    11401 gcagtgcacc ttcgggtaaa atcctatatt gatcttcttt ggtatttcta ttcatgaaca
    11461 cattcactgg gtcgatggta gccatcgtta ccccgatgca cgaggacggg agtcttgatc
    11521 tggatggtat gcgccgcctg gtcgactttc atgtgcagga aggcaccgac gccattgtcg
    11581 tcgtcggaac aacgggcgag tcaccgaccg tcgatgtcga agaacatcgc gagttgatcc
    11641 agcttgttgt cgagtacgca gccggcagaa ttccggtaat cgccggtacg ggtgccaact
    11701 caacggcaga agctatcgag atgacgcatt ttgccaagaa agcgggtgcc aatgccgcgc
    11761 tgtctgtcgt tccttactac aacaggccga cgcaggaagg cctctaccgc cacttcaagg
    11821 caattgctga agctgtggac attcccctca ttctttacaa cgtccccggt cgtaccgtgg
    11881 ccgacatgag cagtgagacg tcattgcgcc tggcgctgat cccgaacatc gtcggaatca
    11941 aggatgccac cggcaatatt gaccgcggga gcgagctgat cgcccgtgcg ccgaaaggct
    12001 ttgcggtcta cagcggtgat gattccagtg cctgcgcgtt gatgttgctg ggtgctcagg
    12061 gcaatatttc ggtagtggcc aatatcgcgc cacgcctgat gcacgaaatg tgcgcagccg
    12121 cactgaaagg cgacctgctc aaggcgcgtg aattgaattt ccgcttgctt ggactgaatc
    12181 gccagctttt ctgcgaggcc aatccgatcc ccgtcaagtg ggcgtgtcag gaactcgggc
    12241 tgatcaagga gggcattcgc cttcctttga ctcccctggc gccggaattc catgaacgcg
    12301 ttcgcacggc catgcggcag gccggtttgt tggcttgatc ccagattccc aggaacaccc
    12361 gatgagattt gctgtttttc gtctgagttt ttgtgccttg gcggtcgtgc tgtcaagctg
    12421 tagcggtatc gggctcgaat ccaaaaagat tgactacaag acatctgcgg cgaccaaggt
    12481 gccgacactc gagattccgc ccgatctgac ttcgccaact cgcgatgatc gtttcgcagt
    12541 gccggacacg gctggcaagg gaacggcaac tttctcggca tactcggggg agcgctcgcc
    12601 acaggcgcta gcgcaacaac aaagtggtgt tctgccgcag gtggacaagg cgcgaatcga
    12661 gcgctcggga aatcagcgct ggctggtcgt tgccggtact cctggaaagc tgtgggacca
    12721 gatcaaggat ttctggcagg agaccggatt cgtgatcaag cttgaattgc ctgaagccgg
    12781 agtaatggaa accgattggg ctgaaaaccg cgccaagatc aaggaagatg ccttgcgtaa
    12841 cctgctgggc aaggtgatcg attccttata ctcgaccgcc gagcgtgaca aattccgcac
    12901 gcgcctggag ccaggtttgg agccgggtac aacggatgtc ttcatcagtc atcgcgggat
    12961 gtatgagatc tacgttaccg agggaaagga tcagaccaag tggcagccac gtgagccaga
    13021 tcctgaactt gaggcagaga tgctgcgtcg gttgatggtg cggcttggga gtgacgataa
    13081 gcgtgctgcg gcacaaattg cggcggccaa ggaaaaaccg gcagatcgcg ccagactctc
    13141 gcgaggcaat gacggtgccg gcacgcttga agtgcaggag tcttttgatc gcgcatggcg
    13201 tcgtgttgga ctggcgcttg atcgtgtcgg cttcaccgtc gaggatcggg accgttcaaa
    13261 gggattgtat ttcgttcgct atgtcgatcc ggaagtcgat gggcagaaaa aggacgatgg
    13321 attcatgtcc aagttggctt tctggaaagc ttccggacct gacccaaaaa ttaagtaccg
    13381 tatctatttg aaagaagttg gttcctcgac gacggttcag gtgctttcgt tcgagggtgg
    13441 tgttgaccaa tctgaaacgt caaagaagat tctgagcctg ctctacgatc aactgaagtg
    13501 atgcgatttg cttcgctggg tagcggcagc cgggggaatt ccctgcttgt cgatgcgggt
    13561 gacaccaagc ttctgctcga ctgcggtttc tccgtgcgtg cgacaactga caagttgggg
    13621 cgactcggtg tttctcctga agaaatctcc ggcatactgg ttacccatga acacggcgac
    13681 cacatcgccg gggtgttcaa gttcgccagc cgtttcagca ctccagtcta tctgacgcac
    13741 ggcacgcaca ttgccgcacc tagcggcaag tcgctgtcga cgtgtcgcat gatcgacagc
    13801 catgcttcct ttacgatcgg taatctggag attcatccat ttccggttcc gcacgatgcg
    13861 cgtgaacccg ttcagtttgc cttctcggac ggggcgcatc gcttgggagt gctgaccgat
    13921 accgggtcga ttacgcagca tgtcattgat gtgctgcgtg tctgcgatgc gttggttctc
    13981 gagtgcaatc acgattcgga gctgctggcg gcttcaagct atccgatgac gttgcagcgc
    14041 cgaatttcgg ggaacttcgg ccatttgagt aacaggcagg cagtttcctt gttgttgcag
    14101 atcgaaacac ggcaactgca gcatatcgtt gcggcacatc tcagcgagca gaataaccgt
    14161 cacgaactgg ttgtagctgc tctggcgaca gcgttgaatt gtgctgatga ttggattggc
    14221 gtggccggtc aggaggaggg ctttggctgg cgccagttgg tttagaagcg gtgtgaacgt
    14281 ttgaagccgg cgtggccggc tgtgtgagaa gtctgtcacc attgccacgc gcagtcccca
    14341 tccccaaaaa acaaatgccc gtcagatcta agcctgacgg gcatttcgca atgacttcgc
    14401 agcagaccat tacgcagcct gcgctgcggg tctgttcaaa gccttgaaga ctacttcttc
    14461 attgcatcct tagcggcgcc catggcggca tcctttgcag caggagtagc atccttcg
//
LOCUS       DAORPM010000023        14170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_855_length_14170_cov_12.337868, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000023 DAORPM010000000
VERSION     DAORPM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_855_length_14170_cov_12.337868"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLD53_02170"
     CDS             <1..384
                     /locus_tag="PLD53_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HRC59741.1"
                     /translation="LLWEHTCFEAFVASVSDTAYREFNFSPSGQWASYAFSDYRLADE
                     TAAMIDSPQITSRLFAGRMELEAVIFPDALLAGSRKLLLGLSAVVEAADTVDGSHSYW
                     ALRHPSPRPDFHHRNSFAFELVKPR"
     gene            404..1615
                     /locus_tag="PLD53_02175"
     CDS             404..1615
                     /locus_tag="PLD53_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRC59742.1"
                     /translation="MSILLFGLDRLLTDAALRKPLAGRQVALLAHPASVAADLTHALD
                     ALTAFPELHLSSAFGPQHGLRGDKQDNMVESSDFVDPRHGIPVFSLYGEVRRPTPAMM
                     DTFDVLLIDLQDLGCRIYTFITTLRYMLEAAAEHGKTVWVLDRPNPAGRPVEGLVLRT
                     GWESFVGAGALPMRHGLTLGELACWFIATLRLDVDCEVISMHGWFPDSAPGYGWPVGK
                     RSWINPSPNAPNLSMTRCYPGTVMLEGTTLSEGRGTTHPLELFGAPDIDARALIAEMF
                     ALAPGWLAGCRLRECGFEPTFHKHAGKLCNGVQIHVDDEHYQHTEFRPWRLQCLAFKA
                     LRRLQPDYPLWRDFPYEYESERLAIDLINGSPLLREWVDDPAATPADLDALAWVDEMA
                     WMTAREDFLLY"
     gene            complement(1666..2847)
                     /locus_tag="PLD53_02180"
     CDS             complement(1666..2847)
                     /locus_tag="PLD53_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HRC59743.1"
                     /translation="MSRDVVVLSAVRSAIGTFGGGLSWIEPCELAGLVMKESIARSGV
                     DPKEINYVTVGTVLPTDSRYAYVSRVASIQAGLPMESIAMQVSRLCSSGLQGIVTSAQ
                     NIMLGDSDYGIGGGVEVMSRATYMLAAMRTGARMGDTKAVDSMVATLNDPFGVGHMGV
                     TAENVATKWGITREEQDAFAVESQQRAGRAIAEGRFKSQIVPIVQQTRKGDVVFDTDE
                     HCKPKTTLESLAKMKPAFSKTGTVTAGNASGINDGAAFFVLAAADVAAKAGHKPIARL
                     VSYAITGVPNEIMGIGPISATKLVLKKAGLTLDQMDVIEANEAFAAQALAVSRDLGLD
                     PAKTNVNGGAIALGHPIGCSGAFIATKALYELQRTNGKYALVTMCIGGGQGIAAIFER
                     L"
     gene            complement(2879..3817)
                     /locus_tag="PLD53_02185"
     CDS             complement(2879..3817)
                     /locus_tag="PLD53_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HRC59744.1"
                     /translation="MNLFSLVAVLLLEQIRPLPYRRVVHEPLARFARFLEGYFNAGER
                     SYGLIAWVISVGGLVLVAGGVYAALNALNPLLAWLWNVLVLYLTMGFRQFSHYYTDIQ
                     LALRMEDVPHARQLLSEWRGRPADDLSSSTIARLAIEEALSASHRHVFGVLVCFFLLP
                     GPCGAVLYRAAAVFADTWGRRNDDAAERFGKFSQQAFAVIDWLPLRITAAGFAIVGNF
                     EDAVYCWRNQADKWPDDQLGSGIGIVLASGAGALGVRLGMPVVEAGEISDRAEIGTGD
                     EADVDFMQSAVGLVWRALMLWLLLLLLLGFASMVGP"
     gene            complement(3981..4502)
                     /locus_tag="PLD53_02190"
     CDS             complement(3981..4502)
                     /locus_tag="PLD53_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRC59745.1"
                     /translation="MQEEDIDDIELTPASVLFPIVLREGGASVLLTQRTEHLKDHPGQ
                     ISFPGGRYEPDDTSPAHTALREAEEEIGLSSAHVEIVGYLPEYLTITGYRVTPVVAIV
                     TPPFDLQPDPYEVAQIFEVPFSFLMNAANHQRHSLPYRGKIRHYVAMPYQGYFIWGAT
                     AGIILTLSKALSA"
     gene            complement(4600..4863)
                     /locus_tag="PLD53_02195"
     CDS             complement(4600..4863)
                     /locus_tag="PLD53_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59746.1"
                     /translation="MPHRDQEILMLRRELEILMGERQTLLRVVGATAALIACLDSKRL
                     PLGAVESADLVATSINALSEETLQDALNAVSAEIEEEETGDRN"
     gene            complement(4873..5481)
                     /locus_tag="PLD53_02200"
     CDS             complement(4873..5481)
                     /locus_tag="PLD53_02200"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HRC59747.1"
                     /translation="MVLATQGVRAADVGLAGLFPGKALLTINGGRPRVVPLGVTTDEG
                     VKVLSIEGETATLEIDGKKRILRVGQNVASQSSTDGPAQAVLTADSGGHFLVTGNING
                     TTVRFIVDTGASMVSLGASDARRIGIDPSKGLPGIANTANGQTMVSRVKLDTVRVGEI
                     VLNNVDAMVHQNDLPIALLGMSFLNRMEMQRNGETMTLKKRY"
     gene            5570..6295
                     /locus_tag="PLD53_02205"
     CDS             5570..6295
                     /locus_tag="PLD53_02205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRC59748.1"
                     /translation="MKPIAIFRHTKTEGPGYFATFLDQRSIPWQLIAIDQDEAVPECA
                     EAFSGLCLMGGPMSVNDQLPWIGQVCALIRDANSRDIPVIGHCLGSQLMSKALGGVVT
                     PSPIKEIGWGHAYTENNDSARLWLRDCLGETGLATVFQWHGETFSIPPGAQRILTNEF
                     CTNQMFTLGPHLAMQCHVEMTSEMINEWCKSWATEVHGLDPRPVTVQTPEQMLSETPD
                     RLPVMRLLADQLYSIWMMGLKRN"
     gene            complement(6364..7074)
                     /gene="rquA"
                     /locus_tag="PLD53_02210"
     CDS             complement(6364..7074)
                     /gene="rquA"
                     /locus_tag="PLD53_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HRC59749.1"
                     /translation="MTKNHASLAIDPYYDGVPAYMTEVYDWAYVDPKWVRVLDHNLVV
                     RVQLFLNDQRLIRAYLNEIKEGMRVWQLAHVYGDLVSRAAHKVGPRGTFHLTDVTPIQ
                     IEHSQRKLDGIEWAKVIRCDAASFSGEESVDYDLISSFFLLHEVPDEKKHEIVNNMLA
                     NLPKGGKAVFVDYHNPARWQPVRYILKLVNRYLEPFADALWKNEISSYAAQADRFTWR
                     KRTFFGGVYQCVLVEHKQ"
     gene            complement(7363..10371)
                     /locus_tag="PLD53_02215"
     CDS             complement(7363..10371)
                     /locus_tag="PLD53_02215"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC59750.1"
                     /translation="MSKTAEDLEESKRINPGVLSWVEELRRVSMAVSKKTIKSSTSRN
                     QLFYLLHWTKDASGFGVTVLKGRDPESAEEWWTIDRALSKPLAFVAEEDVGILRLLWA
                     DRAHDCGLRAFSLGSQHGGDILQRMARTGRLFPENEFSPLLLAEARPATVGWRLDING
                     RQRPCFKPTPAASLVVHLQPPWYVDLDEFVLGPLDVPGNSSVIARLLALPPLSDVEAA
                     LVAEALTELAPELPIPSEYASARLRCIETSPIPVLRLQTLHTHGNRNWRGYVVSYNAG
                     SFDVVLPVFRYHDAEIKPGDVREFSTLPEGETVRILRQFEREKELMGSLTETGLQKIP
                     AYALHTFGSPPDSAYGLENESCWPRFMREALPELRDAGWQIEFDEDFRHHALQVEAWE
                     ADLIESENGWFDLDMGIIVEGERLPLAPLLAALFRSDPRWLDSALLDQIADDEMIELK
                     TPVKLRICAPAGRLKPLAATLIDLFDGFTGGNVLRLSRFDAPRLAELNDTSRWQFRGQ
                     RDVLALADQLSVAQGISHIDSPAGLGLELRSYQKEGLAWLQFLRQQKLSGILADDMGL
                     GKTAQALAHLLTEKESGRLDRPALIVLPTSLIFNWKNEAARFAPSLSILSLHGIERKS
                     RFVEIPQHDLVMTTYPLLWRDSHELTQYRYHLLILDEAQTVKNSRSQGAEVVRKIDAR
                     HRLCLTGTPLENHLGELWSQFDFLLPGFLGNSNTFAKYWRMPIEKQGDAQRRNLLAQR
                     IRPFILRRKKEEVARELPPKTVIVRKIELAGSQRDLYETVRAAMDAMVREEVASKGFG
                     RSQIVILDALLKLRQICCDPRLVKAASAQRVRERAKLDLLMTMLPELVDEGRRILLFS
                     QFTSMLSLIETELKFAGLDYVILKGDTSDRESPVRRFQAGEVPIFLISLKAGGVGLNL
                     TAADTVIHYDPWWNPAVENQATDRAHRLGQDKPVFVYKLIIAGSIEEKILALQERKAD
                     LAAGILSEDRTIDLKFGEEDIAALFAPLPA"
     gene            complement(10542..11036)
                     /locus_tag="PLD53_02220"
     CDS             complement(10542..11036)
                     /locus_tag="PLD53_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRC59751.1"
                     /translation="MPSFDFSSEVDMVALKNAIDSAGKKLIGRHDFKGTSAKLELNDK
                     DKAITVYADSDFQLNQVKDILFPEMEKKEPDCTKRLDHQPVQKVSGDKVKQELKIKIG
                     IEIELAKKIVKLLKDSKLKVQAAIQGDAVRVSGAKRDVLQEAISLVKKAIVDFPIKYG
                     NFRD"
     gene            complement(11049..11378)
                     /locus_tag="PLD53_02225"
     CDS             complement(11049..11378)
                     /locus_tag="PLD53_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HRC59752.1"
                     /translation="MGRSLNMSQFDNVSVVKRANVYFDGKCVSHSLLLADGTKKTVGV
                     ILPSSLVFDTGSPEVMEGIGGSCRVRMKGESDWSSYSTGQSFEVPGNSSFEISCDEPY
                     HYVCHFS"
     gene            complement(11427..12656)
                     /locus_tag="PLD53_02230"
     CDS             complement(11427..12656)
                     /locus_tag="PLD53_02230"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRC59753.1"
                     /translation="MSDIKKAVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEE
                     LEAARPKAIKFGIKPENIYIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIA
                     KRMIDIVNLTGSDAVVHGATGKGNDQVRFELGAYALKPDIKIIAPWREWDLLSREKLL
                     AYAEKHDIPVEMKHKQGGSPYSMDANLLHISYEGRHLEEPSAEAEESMWRWTVSPEAA
                     PDAAEYLDLEFEKGDLVAISGKRMPADQLLALLNQIGGKHGIGRIDLVENRYVGMKSR
                     GCYETPGGTILLRAHRAIESITLDREVAHLKDDLMPRYASLIYNGYWWSPERNALQAL
                     IDHTQQKVNGWVRLKLYKGNVIVAGRDSKTDSLFDSTIATFEDDAGAYDQKDAGGFIK
                     LNALRMRIAAKLDKRKS"
     gene            complement(12671..13606)
                     /gene="argF"
                     /locus_tag="PLD53_02235"
     CDS             complement(12671..13606)
                     /gene="argF"
                     /locus_tag="PLD53_02235"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRC59754.1"
                     /translation="MRIVKPGVKHFLQFKDFTRDEFDYLFERTRWIKEQFKSYQQHRP
                     LIDRTLVMIFEKASTRTRLSFEAGMQQLGGAAIYLNTRDSQLGRGEPVEDAAQVISRM
                     SDLIMIRTFEQETVERLSAYSRVPVINGLTNEYHPCQIMADIYTYIEHRGPIQGKTVA
                     WIGDSNNVCNTWLQAAEVLDFNVRVSTPPGYGVEPERAGLYGTDHFEQIDDPMAAATN
                     ADIVTTDVWTSMGFESENEERKRDFADWQVDADMMRVARHDALFLHCLPAHRGEEVTA
                     EVLDGPQSVVWDEAENRLHVQKALMEYLVLGHMNS"
     gene            complement(13619..>14170)
                     /locus_tag="PLD53_02240"
     CDS             complement(13619..>14170)
                     /locus_tag="PLD53_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC59755.1"
                     /translation="GWLFICDEVQCGMGRTGKWFGFQHAGIRPDVVTLAKGLGSGIPI
                     GACLTAGEAAGLFCPGNHGSTFGGNQLATTAATTTIDVIENDQLIANAVLVGQLIKAG
                     LTQALKEVDGVVDIRGQGLMIGIELDRPCAELVTRGLDAGLLINVTAEKVVRLLPPLN
                     FSAEEGEELVTRLSKLIRDFLAA"
BASE COUNT         3214 a         4190 c         3732 g         3034 t
ORIGIN      
        1 ctgctttggg aacacacctg cttcgaagca ttcgtcgcca gtgtcagcga tacggcgtac
       61 cgcgagttca acttctcgcc ctcaggacaa tgggcatcgt acgcattttc ggattatcgt
      121 cttgccgacg aaacggcagc catgatcgat tcgccgcaaa tcacttcccg cttgttcgcc
      181 ggccggatgg aactggaagc cgtgattttt cctgatgcgc tgcttgcagg gagcaggaag
      241 cttctgcttg ggctttcggc ggtcgtcgaa gcggccgata ccgtcgatgg cagccacagt
      301 tactgggcgt tgcgccatcc ctcgccgcgc cctgattttc atcaccgtaa ttccttcgca
      361 tttgaactgg tgaagcctcg ataatctgct tgaaagcgca acgatgtcca tccttctatt
      421 tggtctcgac cgcctgctca ccgacgccgc gttgcgcaag ccgctggctg ggcgtcaggt
      481 cgcactgctt gcccacccgg cgtcggtcgc ggccgacttg acgcacgccc ttgacgcgtt
      541 gaccgcattc cccgagctgc acctgagctc ggccttcggc ccgcagcacg gcttgcgtgg
      601 cgacaagcag gacaacatgg tcgaatcgtc cgattttgtc gacccgcggc atggcatccc
      661 ggtattcagc ctgtatggcg aagtgcgccg cccgacgccg gcgatgatgg ataccttcga
      721 tgttctgctg atcgatctgc aggatctcgg ctgtcgcatt tacaccttca tcaccactct
      781 gcgctatatg cttgaagcgg ctgccgaaca tgggaagacg gtgtgggtac ttgaccgccc
      841 caacccggcg ggccggcccg tggaaggcct ggtgttgcgg accggctggg aaagctttgt
      901 cggcgctggc gcactgccca tgcgccacgg actgacgtta ggcgagctgg cgtgctggtt
      961 tatcgccact ctcaggctcg acgtcgattg cgaagtgata agcatgcacg gatggttccc
     1021 cgattcggcc cccggttacg gatggccagt tggcaagcgc agctggatca accccagccc
     1081 gaatgcgccc aatctttcaa tgacgcgctg ctaccctggc accgtgatgc tcgaaggcac
     1141 cactctctcg gaagggcgtg gcacgaccca cccgcttgag ctgttcggcg caccggacat
     1201 tgacgcccgg gcactgatcg ccgaaatgtt cgctctcgcg ccgggctggc ttgccggctg
     1261 tcgcctgcgc gaatgcgggt tcgagccgac cttccacaaa catgccggaa agttgtgcaa
     1321 cggcgtgcag attcatgtcg atgacgaaca ctaccagcac accgagttcc gtccctggcg
     1381 actgcaatgt ctggccttca aggccttgcg ccgcttgcag cccgactacc cgctgtggcg
     1441 cgactttccc tacgaatacg aaagcgagcg tctggccatc gacctgatca acggcagccc
     1501 actgttgcgc gaatgggtcg atgatcctgc agcaaccccg gccgacctcg atgcgctggc
     1561 gtgggtcgac gaaatggcct ggatgaccgc ccgcgaagac ttcctgctct actaataaaa
     1621 aaaccgacag gccagccgtc ggttttttta ttgcgaagcg cgtgatcaga gacgttcgaa
     1681 aattgccgcg atcccctgcc cgccaccgat acacatcgtc accagagcat acttgccgtt
     1741 ggtgcgttgc agctcgtaca aggccttggt ggcaatgaaa gcgccggaac aaccaatcgg
     1801 gtggcccaat gcgatagctc caccattcac gttggttttg gccggatcaa gtccaagatc
     1861 gcggctaacc gcaagcgctt gagcggcgaa ggcttcgttg gcttcgatca cgtccatctg
     1921 gtccagcgtc aatccggcct tcttgagtac caacttggtc gccgaaatcg ggccgatgcc
     1981 cataatctcg ttcggcacac cggtgatggc gtacgaaacg agacgtgcga tcggcttgtg
     2041 gccggccttg gccgcaacgt cagcggcagc caacacgaag aaggcagcgc cgtcgttgat
     2101 gcctgaagca ttgccggcgg taaccgtgcc cgttttcgag aacgccggtt tcattttcgc
     2161 cagagactcc aaagtcgtct tgggtttgca gtgctcgtcg gtatcgaaca cgacatcgcc
     2221 cttacgcgtc tgctgaacga tcggaacgat ctgcgacttg aaacgtcctt cggcaatggc
     2281 acgcccggcg cgttgctgcg attcgactgc aaacgcatcc tgttcttcgc gggtaattcc
     2341 ccatttggtg gcaacatttt ccgccgtcac gcccatatgg cctacaccaa acggatcatt
     2401 cagcgtggca accatcgaat cgacagcctt ggtgtccccc atgcgggcgc cagtgcgcat
     2461 cgcagcgagc atgtaggtgg cgcgcgacat gacttcgacg ccaccgccaa ttccgtaatc
     2521 actgtcaccc agcatgatgt tctgtgccga agtgacgata ccctgcaaac cggaagaaca
     2581 gaggcggctg acctgcatgg cgatcgactc catcggcaaa ccagcctgaa ttgaggcaac
     2641 gcgcgagacg taggcgtagc gtgaatcggt cggaaggacg gtgcctaccg ttacatagtt
     2701 gatttccttc ggatcaacac cggaacgtgc aatcgactcc ttcatgacga ggccggccag
     2761 ctcgcatggc tcaatccagg atagtccacc gccgaaggta ccgatcgccg aacgaactgc
     2821 gcttaaaacg acaacatctc tgctcatgtt tacaatctcc taagtgtata tgtatcgatt
     2881 acggacccac catactagca aaacccaaca acagcaacag caacaaccag agcatcaagg
     2941 cacgccagac caagccgaca gcactttgca tgaaatcgac atccgcttca tcgccagtgc
     3001 cgatctcggc gcgatcagaa atctcgccgg cctcgaccac cggcatgccc agtcgaacac
     3061 ccagcgcacc agcaccgctt gccagaacga taccgatacc actacccagc tggtcatccg
     3121 gccatttgtc ggcttgattt cgccaacagt aaactgcatc ttcaaaattg ccgacaatgg
     3181 caaacccggc ggcagtaatc cgcaagggca gccagtctat aacagcaaag gcctgttgtg
     3241 aaaattttcc gaaacgctcg gcggcgtcgt cgtttcttct tccccaggta tcggcaaaga
     3301 cagccgccgc ccgataaagg acggcgccgc atggccccgg aagcaggaag aagcagacaa
     3361 gcacaccaaa cacgtgacga tgcgatgcgc tgagtgcttc ctcaatcgcc agccgagcaa
     3421 tggtggaaga cgaaagatcg tcggccggcc gcccccgcca ctcagacaaa agctgacgcg
     3481 cgtggggcac atcttccatg cgcagagcga gttggatgtc ggtgtaatag tgactgaact
     3541 gccggaagcc catcgttaga tacagaacaa gcacattcca gagccaggcc agcagcgggt
     3601 tgagtgcgtt tagcgctgca tatacgccgc cggcaaccaa aaccagcccg cctacggaaa
     3661 tcacccaggc aatcaggccg tagcttctct cacctgcgtt gaagtagcct tcaagaaatc
     3721 gagcgaacct ggcaagcggc tcatgaacaa cacgacgata aggcaatggc cgaatctgct
     3781 caagcaacag aacggcaacc agagaaaaaa gattcatgcg aaatatgtga ttgcgcggcg
     3841 aagagcggtc tgtgcccgtc gggcatcgga atcggggaag ataccatatt taaacagtgc
     3901 ctgcagaggc cttcagcacg tccaaccgag tctccggctt cagcgaatca ggcaaaaggt
     3961 ggctctgacg ttcgactgat tcaggccgac aatgccttgg agagcgtcaa gatgataccg
     4021 gccgtggcac cccagatgaa gtacccctgg tagggcatgg caacgtaatg ccgaattttt
     4081 ccacgatagg gcaaggagtg gcgctgatgg tttgccgcgt tcatcaggaa agagaacggt
     4141 acctcgaaaa tctgcgcgac ttcgtagggg tcgggctgca aatcaaatgg cggcgtcacg
     4201 atcgccacta cgggcgttac gcgataccct gtgatggtga ggtactcggg cagatagccg
     4261 acaatttcaa cgtgcgcaga tgacaaaccg atttcttctt cagcttcacg caatgcggtg
     4321 tgcgctggcg aagtatcgtc aggttcgtaa cggccgcctg gaaaactgat ctgacccgga
     4381 tgatccttta agtgttccgt acgctgggtg agcaacacag acgctcctcc ctcccgcaag
     4441 acaatgggga agagcaccga agccggtgtc agttcaatgt catcaatatc ctcctcctgc
     4501 acgaatgcgg tccgcgtcgg gaccgggtac aacaccgacc gcagatgttc aaggtcgatt
     4561 gcgtccggac tcgcgctggt agatgattga acgatagcat cagtttcggt cgccagtttc
     4621 ctcctcctcg atttcggcac tcacggcatt gagggcgtcc tgcaatgttt cctcggacag
     4681 cgcgttaatc gacgtcgcca ccagatcggc agactccacg gcacccaatg gcaagcgctt
     4741 gctgtcaagg catgcgatca gggcggcagt ggcacccaca acccgtagaa gggtctgcct
     4801 ctcacccatc aatatctcaa gctcgcggcg caacatgagg atttcctggt cgcgatgggg
     4861 catgcaattc tcctagtaac gcttcttaag tgtcatcgtc tcgccattcc gctgcatttc
     4921 cattcgattc aaaaaactca tgccgagcaa tgcgatgggc agatcgttct gatgcaccat
     4981 ggcgtcgaca ttattgagga caatttcgcc gacgcgcact gtatccagct tgacacgcga
     5041 caccatcgtc tgaccattgg ctgtattggc tattccgggc agccccttgc tggggtcaat
     5101 gccgatccgg cgtgcatcgc tcgctcccag ggaaaccata gaggcgccgg tgtccacgat
     5161 aaaccgaacg gtcgtcccat tgatgtttcc ggtcaccaga aaatgcccac ctgaatcggc
     5221 agttagcaca gcctgggccg gcccatcggt ggacgactgc gacgcaacgt tctgtccaac
     5281 acgcaaaatc cgtttctttc catcaatttc aagagtcgct gtttcgcctt cgatggaaag
     5341 cactttcact ccttcgtcgg ttgtaacgcc gaggggaacg acacgcggcc tgccgccatt
     5401 gatggtcaac agagccttgc ccggaaacag gccagcgagg ccgacatccg cagcacgtac
     5461 gccttgagtg gccaagacca ctccggcaaa cagtaggcaa cgtgctaccc agcgcgtaaa
     5521 attcgcggcg tgcacggaag cgcaacccga tctcggaaag ctcacgaaca tgaaacccat
     5581 agccattttt cgccacacta aaaccgaggg accaggctat tttgccacat tcctcgatca
     5641 gcgctcgatt ccatggcagc tgattgccat tgatcaggac gaggcggtac ccgaatgcgc
     5701 agaagcattc tccggactgt gcctgatggg tgggccaatg agcgtcaacg accagttgcc
     5761 gtggatcggt caagtgtgcg cactgattcg tgatgccaac tcaagagaca tcccggtcat
     5821 tggccattgc ctaggtagcc agttgatgag caaggctctg ggtggtgttg tcacgccaag
     5881 cccgatcaag gagatcggct ggggccacgc ctataccgaa aacaatgata gtgcacggct
     5941 atggctacgt gattgtcttg gcgaaaccgg cttggcaacg gtatttcagt ggcatggtga
     6001 gactttcagc attccacccg gtgcgcaacg catcctcacc aacgaattct gcaccaacca
     6061 gatgttcaca ctcggcccgc acctggcgat gcagtgccat gtcgaaatga cctctgaaat
     6121 gatcaatgaa tggtgcaagt cctgggccac cgaagtgcat ggcctggatc cccggccagt
     6181 tacagtgcag acaccggaac aaatgctgtc tgagaccccg gatcgcctgc cagtcatgcg
     6241 gctactggcc gaccagttgt actcgatctg gatgatgggc ttgaagcgga attgacttcg
     6301 atcgatccgc tgctttcagg aacaaacgta gtgtactggc ggtggccgtg tcaccgttgt
     6361 tacctactgt ttgtgctcaa ccaagacgca ctgatagaca ccaccgaaaa aggtgcgctt
     6421 acgccacgta aaacgatcgg cctgagcagc ataactactg atctcgtttt tccatagcgc
     6481 gtcagcaaat ggttccagat agcggttgac gagttttagt atgtagcgaa ctggctgcca
     6541 tctcgcaggg ttgtggtagt caacgaacac tgccttgccg cctttgggca gattcgccag
     6601 catgttattg acgatttcgt gctttttctc gtcaggaact tcgtgcaaaa ggaaaaagct
     6661 actgatcagg tcgtaatcca ccgattcttc gcctgaaaaa ctggctgcat cgcatcgaat
     6721 gacttttgcc cattcgatgc cgtccagttt gcgctggcta tgctcaatct gtatcggagt
     6781 tacgtcggtc agatggaagg tgccgcgcgg ccccactttg tgtgccgcac gagataccag
     6841 atcgccatac acgtgcgcca gttgccagac acgcatgcct tccttgattt cgttcaaata
     6901 ggcacggata agccgctggt cattcaggaa caactgcaca cgcacaacca ggttgtgatc
     6961 cagaacacga acccacttgg gatcgacata tgcccaatcg tagacctcgg tcatgtacgc
     7021 gggaacgcca tcgtaatagg ggtcaatggc gagtgacgca tgattcttgg tcatttattg
     7081 gtccgatcta gtcaaagtcc catgcatttg gcgtaaacac ggtcaccaac aaagccagcg
     7141 tagaaagcca aacagaggaa ggggcacaaa gactttcatt atcgctgagc tgcgtattcc
     7201 agtcaaaccg ctatggctga acgcgagtca attctttcat ctctaatacg ccggtggatt
     7261 ctggaatttc tgctctcttt cgggctttca gcgggatttc ctccagcagt actcgctgca
     7321 aaccttgcgg tcgctattga tgactattgt gcattccatg tatcaagcag gaagtggcgc
     7381 gaatagggct gcgatgtctt cttcaccgaa tttcaggtcg atcgtacgat cttctgacaa
     7441 gatcccggca gcgaggtctg ccttgcgctc ctgcaatgcg agaatctttt cctcaatact
     7501 gccagcgatg atcagcttgt agacgaacac cggcttgtcc tgcccgagcc gatgagcacg
     7561 atcagtcgcc tgattctcaa cggcggggtt ccaccacgga tcgtagtgaa tcacggtatc
     7621 ggccgcggta agattgaggc cgacgccacc ggctttcagg ctgatcagga aaattggaac
     7681 ttcgcccgcc tggaatcgac gcaccggcga ttcccggtcg gaggtgtctc ccttcaggat
     7741 gacatagtca aggccagcaa acttcagctc agtctcaatc aatgacaaca tgctggtaaa
     7801 ttgcgaaaaa agcagaatgc gacggccctc atcaaccagc tcgggcaaca tggtcatcag
     7861 cagatccagc ttggcccgct ccctgacccg ttgcgcagaa gcagccttga ccaaccgggg
     7921 atcacaacag atctgcctca gtttgagtag cgcatccagg ataacgatct ggctgcgccc
     7981 gaatcctttg ctggcgacct cctccctcac catggcgtcc attgctgcgc ggacggtctc
     8041 gtaaaggtca cgctggctcc ccgcgagttc aattttgcgc acgatgacgg ttttgggcgg
     8101 caattcgcgc gccacttctt cttttttgcg gcgcaggatg aaggggcgta tccgctgggc
     8161 cagcaggttc cggcgctgcg catcgccttg cttttcgatc ggcatgcgcc agtacttggc
     8221 aaaggtattg ctgttgccca gaaaaccggg caacaggaaa tcgaactgac tccacaattc
     8281 gcccagatgg ttttccaacg gcgtgccggt caggcaaagg cggtgccgtg cgtcgatctt
     8341 gcgcacgacc tcagctcctt gacttcgaga gttcttgact gtctgtgctt cgtcaagaat
     8401 gaggagatgg tagcggtact gggtcagctc gtgtgaatcg cgccacagca aaggatatgt
     8461 cgtcatcacc agatcatgct gcggaatttc aacaaaccgg ctcttgcgtt cgatgccatg
     8521 cagggaaagg atcgacaggc ttggcgcaaa gcgagccgcc tcgttcttcc agttgaaaat
     8581 gagagacgtg ggcaagacta tcagtgccgg acgatccaga cgcccggatt ccttttccgt
     8641 cagcagatgt gcgagggcct gtgctgtttt gccaaggccc atatcgtccg ccagaattcc
     8701 ggacaacttc tgctggcgca gaaactgcag ccaggccaaa ccctccttct ggtaggagcg
     8761 caactcaagc cccaaaccgg cgggtgagtc gatgtgactg ataccctggg ccaccgacaa
     8821 ctggtcagcg agcgccagca catcgcgctg accgcggaac tgccagcggc tggtgtcgtt
     8881 cagttcagca aggcgcggcg catcaaaacg tgagaggcgc agaacgttgc caccggtgaa
     8941 gccgtcgaaa agatcaatca aggtcgccgc gagcggcttc agacgtccag ctggggcgca
     9001 aattcgcagt ttgaccggtg tcttgagttc gatcatttca tcgtcggcaa tctgatcaag
     9061 cagtgctgaa tccagccagc gtggatcact ccggaacaag gccgcgagca gaggcgccag
     9121 tggcaaacgt tcgccttcga cgatgatgcc catgtcgaga tcaaaccagc cgttttcaga
     9181 ctcaatcaga tcggcttccc acgcctcgac ttgaagcgcg tgatgccgaa agtcttcatc
     9241 aaactcgatc tgccagccgg catcgcgaag ctctggcaac gcttcacgca tgaagcgcgg
     9301 ccagcacgat tcattttcca gaccgtatgc gctatcgggt ggactgccaa aagtatgtag
     9361 tgcataagcc gggattttct gcagccctgt ttcagtcaag ctgcccatca attccttttc
     9421 tcgctcgaac tgacgaagta tgcgtacggt ctccccttca ggcagcgtgg aaaactctcg
     9481 aacgtcgccc ggcttgatct ccgcatcgtg ataacgaaac accggtaaca ccacgtcgaa
     9541 agagcccgcg ttatagctga cgacgtagcc gcgccagttc ctgttgccat gcgtatggag
     9601 tgtctgcaaa cgcaataccg gaatgggact ggtttcaata cagcgtagac gggcactggc
     9661 atactcggaa ggaatcggca attcgggcgc aagctcagtc agcgcttcgg caaccagtgc
     9721 tgcctcaaca tcggacaatg gtggtagcgc taacaggcgc gcaatgactg aagagttgcc
     9781 aggcacatcc aagggaccca agacaaactc atctaggtcg acataccagg ggggctgcag
     9841 gtgcaccact agactggctg caggtgtcgg tttgaaacag ggcctttgcc gtccgttgat
     9901 atcaagccgc caacccacag tcgccgggcg cgcctcggcc aacagcagcg gcgaaaactc
     9961 attctccgga aaaaggcggc cggtccgtgc catccgctgc aggatatctc ccccgtgctg
    10021 cgaacccaga ctgaaggccc gcagaccgca atcatgcgcc cggtcggccc aaagcagacg
    10081 cagaataccg acatcctctt cggcaacaaa agccagtggt ttgctcagtg cacgatcgat
    10141 ggtccaccat tcttcggcac tctcaggatc acggcccttg agaacggtga ccccgaaacc
    10201 gctggcgtcc tttgtccagt gcaagagata gaacaactga tttcgacttg tggatgactt
    10261 gatggtcttc ttgctgaccg ccatcgacac acgacgaagt tcttccaccc aggaaagaac
    10321 gccgggattt atgcgtttgc tttcttccaa atcttcagca gtctttgaca acaggggtat
    10381 cagagctcaa ttgtggaaac aacgccatgt ctcggcaaac acacatacca agaactcgca
    10441 ggaaatatct cacgtagccg gccgggaacc agtcaagcga ccaccacaca tgctttccgc
    10501 ataaacagcc agccgtccaa cgcggctctc aataacagat atcaatcgcg aaaattgccg
    10561 tacttgatcg ggaaatcgac aatggccttc tttaccagac tgatggcttc ctgcaggaca
    10621 tcgcgcttgg caccactgac acgcacagca tctccctgaa tggcagcctg caccttgagt
    10681 tttgaatcct tcaacaactt gacaatcttt tttgccaact cgatctctat gccgatcttg
    10741 atcttcagct cctgcttgac cttgtcgccg gatactttct gcaccggctg atgatccagg
    10801 cgtttggtac agtccggttc cttcttttcc atctctggga aaagaatgtc cttgacctga
    10861 tttagttgaa agtccgagtc tgcgtacact gtgattgcct tgtccttatc gttcaactca
    10921 agcttggcac ttgtgccctt gaagtcgtgg cgcccgataa gtttctttcc agctgaatcg
    10981 atggcatttt tcagcgccac catgtccact tcagaagaaa aatcgaaaga tggcatcaaa
    11041 gcctccgctt aactgaaatg acaaacgtaa tgataaggct cgtcgcaaga aatctcgaac
    11101 gacgagtttc ccggcacctc gaatgattgc cctgtgctgt agctgctcca atcactctcc
    11161 cccttcatgc gtacccgaca ggagccgccg atgccttcca tgacttcggg cgatccggtg
    11221 tcgaagacca acgatgaggg cagaatgacc cccactgttt tctttgtgcc gtccgctaac
    11281 agcagggaat ggcttacaca tttaccatcg aagtaaacat tggctcgctt gacgacactc
    11341 acattatcaa actgcgacat gttcagagac ctccccatag ttgaacgacg agcccggtct
    11401 tgcggccgag ccaatatcgc aaagaatcaa ctcttgcgtt tgtcaagctt ggccgcaatg
    11461 cgcattcgca gcgcattgag cttgatgaag ccaccggcat ccttctggtc gtatgcgccg
    11521 gcatcatcct caaaagtagc aatggtcgaa tcgaacagtg aatcggtctt tgaatctcgg
    11581 cctgcaacga tgacgttacc cttgtagagt ttgaggcgaa cccaaccgtt gactttctgt
    11641 tgagtatggt caatcagcgc ctgcaaggca ttgcgttcag ggctccacca atagccgttg
    11701 taaatcaggc tggcgtaacg cggcatgagg tcgtccttga gatgggcaac ctcgcgatcc
    11761 agtgtgatcg actcgatagc acggtgagca cgaagaagaa tcgtgccacc gggtgtttca
    11821 tagcaaccgc gcgatttcat gccgacataa cgattctcga caagatcgat acgaccgatg
    11881 ccatgttttc caccgatctg attgagcagg gccagcaatt ggtcagccgg catgcgcttg
    11941 ccgctgatgg caacaaggtc gcccttctcg aattcaagat cgaggtattc tgctgcgtcg
    12001 ggcgccgctt ccggcgacac cgtccagcgc cacatcgact cctcagcctc ggcagaaggc
    12061 tcctccaggt gacgaccttc atagctgata tgcagaagat tggcatccat agaatagggc
    12121 gagccaccct gcttgtgctt catctcgacc ggtatgtcat gcttctcggc ataggccaga
    12181 agcttctcgc gcgaaagaag atcccattcc cgccatggcg caatgatctt tatgtcgggc
    12241 ttgagtgcat aggcaccgag ttcaaaacgt acctgatcat tgcccttgcc ggtcgccccg
    12301 tgcacgaccg catccgatcc cgtcaggtta acaatgtcaa tcatccgctt tgcgatcaac
    12361 ggtcgcgcaa ttgacgtacc cagcagatac tcgccttcgt agaccgtatt ggcccggaac
    12421 atcgggaaaa cgaagtcgcg tacgaattct tcacgcagat cgtcgatgta gatgttctcg
    12481 ggtttgatgc cgaacttgat cgcctttggg cgtgccgcct ccagttcctc accctgcccc
    12541 agatcggccg tgaaggtcac gacttcgcac tgataggtat cctgcaacca cttgagaatc
    12601 accgacgtat ccagacctcc ggaatatgcc agaactgcct tcttgatgtc actcattcaa
    12661 aactccagac ttacgaattc atatgaccga gtaccagata ctccatcaac gctttttgta
    12721 catgtagacg attctccgcc tcatcccaga cgacgctctg cgggccgtcg agaacttcgg
    12781 cggtaacctc ttcaccacga tgcgccggca ggcaatgcag gaacagcgca tcgtgtcggg
    12841 cgacacgcat catatccgcg tccacttgcc agtcggcaaa atcacgcttg cgctcttcgt
    12901 tctcggattc aaaacccatt gaagtccaca catcggtcgt cacgatatcg gcgttcgttg
    12961 ccgccgccat tggatcgtcg atctgctcga agtggtcagt accgtatagc cccgcacgct
    13021 ccggttccac cccatatccc ggcggggtcg atacgcggac attgaaatcc agtacttcgg
    13081 cggcctgcaa ccatgtattg caaacattgt ttgaatcacc gatccaggcc accgttttcc
    13141 cttgaatcgg gcctcggtgc tcaatatagg tatagatatc cgccatgatc tggcacggat
    13201 ggtattcgtt ggttaacccg ttgatcaccg gcacgcggga atacgccgaa aggcgttcga
    13261 cggtttcctg ctcgaaggta cggatcatga tcaggtcgct catgcgcgag atgacctgcg
    13321 ccgcgtcttc gaccggctcg ccgcgaccga gttgagaatc gcgcgtattc aaatagatcg
    13381 ccgcaccacc aagctgctgc attccagcct cgaatgaaag gcgagtgcgg gtactggcct
    13441 tctcgaaaat catcaccaat gtgcggtcaa tcaacggccg atgctgttga taggacttga
    13501 actgttcctt gatccagcgc gtgcgctcga acaggtaatc gaactcatcc cgtgtaaagt
    13561 ccttgaactg caggaagtgt ttgacgcctg gcttgacaat tctcatggcg gtccctgttc
    13621 aggcagcaag aaaatcacga atcaatttgg aaagccgcgt tacgagctct tccccttctt
    13681 ccgcactgaa gtttagcggg ggcagcaaac gcacgacctt ttcggcggtt acattgatca
    13741 atagcccggc gtccaggccg cgtgtcacca gctcagcgca gggacgatcc aattcgatgc
    13801 cgatcatcaa accctgacca cggatatcga caaccccatc aacttctttc agtgcctgtg
    13861 tcagtcctgc cttgatcaat tgcccgacca atacggcatt ggcaatcaat tgatcgtttt
    13921 cgatcacgtc gatcgtggtc gtcgctgccg tagtcgccaa ctggttgccg ccaaaggtcg
    13981 acccgtgatt gcctggacag aagagtcctg cggcttcacc agcagtaagg caagcaccga
    14041 tcggaatacc gctcccgagc cccttggcca aggtcaccac atctgggcga atgccggcat
    14101 gctggaagcc gaaccactta ccggtacggc ccatgccaca ctgcacttcg tcgcagataa
    14161 acagccaacc
//
LOCUS       DAORPM010000024        14157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_858_length_14157_cov_10.072472, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000024 DAORPM010000000
VERSION     DAORPM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_14157_cov_10.072472"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLD53_02245"
     CDS             <1..559
                     /locus_tag="PLD53_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59756.1"
                     /translation="HTVSKGESEIAFILDDGSSRCLIDVEGAEILTRHKSTWTEGRFR
                     KTEWTLNIDDEIYALGEFITLGGSRLELDANHDVNALLAEWKRDQPGLLKRFDLDGDG
                     SISMKEWMLARQAARREVAKLHREVRNDADLHTLRCPTNGQLYLLSNIGPERISRRYL
                     WWSIFHLSVFFVSLGALPWIWHNAV"
     gene            619..1488
                     /locus_tag="PLD53_02250"
     CDS             619..1488
                     /locus_tag="PLD53_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRC59757.1"
                     /translation="MQIRFLLPLLSALVLSACASKPPTPPPLTPKPVKIGLALGGGAA
                     RGFAHIGVIKVLEAQGIVPDIIVGTSAGAVVGALYASGHNGFDLQKLAHKMDESKISD
                     WSLPDRGVLKGEALQQFVNDAVAQRPLEKLKKPFAAVATDLNSGESILFRSGNTGMAV
                     RASATVPGVFKPVSINGHEYVDGGLSSLIPVRGARSMGADVVIAVDISARPGNQTVRG
                     TLDILLQTFTIMGQNLARYELKEADIVIRPQVGNVGSTDFPARHDAILEGEKAAQSAL
                     PQIREAIRKASGI"
     gene            complement(1511..3427)
                     /locus_tag="PLD53_02255"
     CDS             complement(1511..3427)
                     /locus_tag="PLD53_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC59758.1"
                     /translation="MPQLILSDASLAYGHVPLLDHADFQLDPGERVALIGRNGTGKSS
                     LLRALAGQGTLDDGTVWQQPGIRRGYVPQEPPFDPDRSVFEAVVDGMGEVSTLLAEYH
                     AVAHAMAEDSADHEALLERMHQLQGELESLNAWSFEMQAERVIQRFSLDPDARVGTLS
                     GGQKKRLALAQALAISPELLLLDEPTNHLDIGAIEWLEEFLVSSGVSMVFITHDRRFL
                     DRVSTRIVELDRGRLLSCPGNFSAYLARKESILHDEAIQNAKFDKFLAQEEVWIRKGV
                     EARRTRNEGRVLRLEQLRRERVARRERQGKVELALDAGDKSGKLVAELEHVSKHFGEG
                     AQRLTVVRDFSCRLQRGDKIGLIGPNGAGKTTLLRLILGELAPDSGRVRLGTKIEVAY
                     FDQFRTQLDEEAALVDVISPGSDYVEIGNEKKHVIGYLGDFLFAPQRARSPVKSLSGG
                     ERNRLLLARLFARPANVLVLDEPTNDLDIETLELLEQLLQDYTGTLFLVSHDRAFIDN
                     VVTQSIAFEGEGVWHEYAGGYQDWADYQARRRAQEAAQPVAKREASGAVQSAKSKADG
                     GRKMSYKETRELAELPGRITSLEQEQARINQRLADPTIYQAEPQEAQRISLRLTEIDD
                     ELMALLERWEALEQ"
     gene            3539..3613
                     /locus_tag="PLD53_02260"
     tRNA            3539..3613
                     /locus_tag="PLD53_02260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3572..3574,aa:Arg,seq:cct)
     gene            complement(3735..6311)
                     /locus_tag="PLD53_02265"
     CDS             complement(3735..6311)
                     /locus_tag="PLD53_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRC59759.1"
                     /translation="MKIALSVLNALSLPAFVLDTEHKVVVWNAPCELLTGIPARDVVG
                     TRDHWRGFYDDPRPCMADLVLDSMLDEASRYYSRHEQTEFASDGHKAEGWFDNINGNR
                     RYLAFEARPVFDDGRIIGAIEVLQDITHHQEAEDQLKLSASVFENSLEGIIITGADNR
                     AISVNKALESLTGYRRDEIIGRNPKMFASNRLPDSFYRKMWKTLARLGYWQGEIWNKK
                     KTGEEYLVRVHLSAVKTGANVTNYIGLLSDITESNRVMEKMEHMAHHDFLTGLPNRSL
                     LEDRLRQALARAVRNRSKFAVMFIDLDKFKAVNDTLGHHIGDALLKEVTRRIQGCLRA
                     SDTISRQGGDEFVLLLEDIGDEADVTHTARKLLAAIGSSCRLGGHVVTVTPSIGISLF
                     PTDGLSVTDLLSHSDAAMYHAKNQGRNNFQFFTERINAKALETLMVENGLRKAIPDGL
                     LLHYQPQLCLVSKSVHGAEALVRWSHPDLGLISPARFIPIAEESGLICEIGEWVLREA
                     CRVMRSTGINMSVNLSPMQLTQSDIVAKVIEALDGMAGYRLTLEITESAFINDFEKTK
                     TTLLALKKIGVNLALDDFGTGYSSLSYLYQLPFDFLKIDQSFISEPKNSPIVLAILEM
                     CNKLNIMTVAEGVESPEQVAFLEANGCDVIQGYYFSRPLPLQTLTGFAQNPPYQVSSK
                     PRVKKLAEEDSNPMLAWSFTYETGHAGIDSQHREILRILDRLDFSARSVDDGTNSRNV
                     MKALIDFVIDHFSYEEKLMHDHGYPWANEHHAEHLDLVETLASYDLQLKANAQVSPSQ
                     LTTSLHTWLMHHILASDKKLGEYLSKHSEPGSESDASDPMQEFTTSWCGFASDGPLMP
                     SD"
     gene            complement(6514..8211)
                     /locus_tag="PLD53_02270"
     CDS             complement(6514..8211)
                     /locus_tag="PLD53_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRC59760.1"
                     /translation="MNGVSWKLRGTMLLLVACAGLAAWLAVNVFGGGMQGWSIAYGLL
                     VVVAVVLSLLIESGLVRRLNSLRGVIGQMYMDGDLTRRAQVEGGDEVSQTAAEFNKLM
                     ESIQTIIGKVFFNSVEVAHASQKLIAEANRVASGSNKQRDAALATSGAMTQLTENMNQ
                     VSQNATETAGISETSSNLSAEGMTIVHNASAEMERISSSVTQSAQVVYALGERSKAIS
                     GIVQTIREIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTAKATGEISQM
                     IGAIQGETQSAITSIEAGTGQAKNGADLAQQAAASLESINSGARVTMEKVDAIAAAVA
                     QQSRAGNTIADHVTNIMQMAENNSAAAEKALGEANQLDYLATNLKEIGNVFKLGPAGD
                     KAVETHAKMPDLVQRAAQAVSQIFEKAVDSGRIKLEDLFDDKYQPISNTKPPKYKTRY
                     DDFTDQNVSPTQEQLLEQNKWVVYAIACDQKGYVPTHNRKFSQPLTGNEKTDFVNNRT
                     KRMFDDPVGRRCGAHDQPFLLQTYRRDTGEIMHDISAPVYVKGRHWGGFRIGYKTEV"
     gene            8604..9017
                     /locus_tag="PLD53_02275"
     CDS             8604..9017
                     /locus_tag="PLD53_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HRC59761.1"
                     /translation="MDMQLAEWSDKLEFGLPRIDSQHKQLFDLAATFAGNGDQIRVMK
                     SLVMLSNYAKVHLRDEEEMLASLKYPELEAHKRLHAEFKDMLIKLLDNARQLSLDEIA
                     KEVQYLIYGWFYNHIMQADVEYVPWVRTHSLKALG"
     gene            9269..12745
                     /gene="mfd"
                     /locus_tag="PLD53_02280"
     CDS             9269..12745
                     /gene="mfd"
                     /locus_tag="PLD53_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRC59762.1"
                     /translation="MPSFKPGERTQLPPFSGSADALHIAQLADQAGQSRLLAVLTASA
                     GDAQRLLEEIPWFAPELRVRLLPDWETLPYDSFSPHHDLVSERLATLYGVMRNECDVL
                     LVPASTAVYRLMPPSYLAAYTFFLKKGKRLDSEQFRSQLTLAGYAHVAQVVAPGEYSV
                     RGGLVDLFPMGSALPFRLDLFDDEIENIKTFDIDTQRTLYPVQEIRLLPAREFPLDDK
                     GRTRFRQRYREIFEGDPARSGIYKDVSSGIAPAGIEYWLPLFFEDTATLFDYLPGDTL
                     LCLHNDVPEAIRGFWRDTQSRHAMLSGERSRPLLPPGDLFLSEEAFFVAARPHARVIL
                     ATGADGPATALPPVAVARRADDPLSALKAFITGFPGRVLLLAESAGRRETLSALFADY
                     GLKPDPCSDLGEFLVGESRLALGVSPLHDGFLLTTNTTSTSQPDQWAFITETELYAGS
                     PSRRSRHAAQRKATLDNWLRDLTELKVGDPVVHEQHGIARYRGLVHLDLGEGDMEFLE
                     LHYADEAKLFVPVSQLHVISRYSASDPDAAPLHTLGTPQWEKAKRRAALQARDTAAEL
                     MALYALRAARQGHAFEFTAHDYDAFADGFGFEETPDQAAAINAVIDDMKSGRPMDRLV
                     CGDVGFGKTEVALRAAFCAVAGGKQVAVLCPTTLLCEQHFQTFSDRFAQVGNNWPIRI
                     AELSRFKTGKESAQALKELAEGKVDIVIGTHKLLQKDVKFSRLGLVVIDEEHRFGVRQ
                     KEALKALRAEVDVLTLTATPIPRSLGMSLEGLRDFSVIATAPQKRLAIKTFVSNFSDG
                     IIREALLREFKRGGQVYFLHNEVNTIENMREKLAKLVPEARIVVGHGQMSERELERVM
                     RDFTQQRVNLLLCTTIIETGIDNPHANTIIINRAEKFGLAQLHQLRGRVGRSHHQAYA
                     YLLTHAGLDESAGKLAGLTKQARQRLEAIQLMDELGAGFYLAMHDLEIRGAGEVLGES
                     QSGEMQEIGFNLFSEMLKRAVASLKQGQEPDLSQPLGIATEINLHTPALLPNDYAPDV
                     NERLILYKRLANGESLDDITDLHEELVDRFGELPPQAQSLLDSHRLRLLCKPLGIIKL
                     DATSEQIVVQFDPEIAKYSPIEPIRIIELIQNDKNYRLAGQDKLALKRHCPTLADRVS
                     AVKDLLRQLTKK"
     gene            12764..13837
                     /locus_tag="PLD53_02285"
     CDS             12764..13837
                     /locus_tag="PLD53_02285"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRC59763.1"
                     /translation="MNTIDIENINVSAFDPMPSPEDILADLPLSTEAARTVMRGRETL
                     RNILERKDHRLFVVVGPCSIHDPLAGIDYARRLRKLSDEVSETLFLVMRVYFEKPRTT
                     TGWKGYINDPDMNDSFTVDQGMKRARQFLLDVAEAGLATGTEALDPISPQYLGDLMAW
                     TAIGARTTESQTHREIASGLSTPVGFKNATSGDVDIAINAIISSSRPHSFLGINGQGR
                     TSIVRTRGNRYCHVVLRGGDRRPNYDTVSVQMTEQALIAAKLPPNIVVDCSHANSYKK
                     PELQPLVMTDVVNQVRLGNKSLVGMMIESNLVGGNQPIPADRSQLKYGCSVTDPCIDW
                     DTTEKTIREAALQLRDILPKRNS"
     gene            13937..>14157
                     /locus_tag="PLD53_02290"
     CDS             13937..>14157
                     /locus_tag="PLD53_02290"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRC59764.1"
                     /translation="MTLCLGGEALELSNLSPIKENRLPSTSPLIRPFAGLRPRSELAA
                     AVAAPPYDVLSSEEARQRAAGKPYSFLHIS"
BASE COUNT         2919 a         4381 c         3932 g         2925 t
ORIGIN      
        1 gcataccgtc agcaagggtg aaagcgaaat tgcattcatt ctcgacgatg gcagcagccg
       61 ctgtttgatc gatgtggagg gcgccgagat cctgacccgg cacaagtcca cttggacgga
      121 aggacgcttc cggaagaccg aatggacgtt gaacatcgac gacgagatat acgcgctggg
      181 tgaattcata accctgggag gcagcaggct tgaacttgat gcgaatcacg acgtcaatgc
      241 cctgcttgcc gaatggaaac gggaccagcc tggcttgctc aaacgcttcg atcttgacgg
      301 cgatggcagc atcagcatga aagaatggat gctggcgcgc caggcggctc ggcgcgaagt
      361 tgccaaattg caccgtgaag tgcgcaacga cgccgacttg cacaccctgc gctgcccgac
      421 caacgggcaa ctgtatctgc tctcgaacat agggcccgaa aggatttcac gacgctatct
      481 ctggtggtcg atcttccacc tctccgtatt ctttgtttcg cttggtgccc tgccctggat
      541 ctggcataac gccgtctgaa atacggtata ggtcgacaag ttcgaagtta gaatgcgacc
      601 tcaccaatcg agtacaccat gcaaatcagg ttcctgctac ccctgctctc cgctctcgtt
      661 ctttccgcct gcgcgagcaa gccaccaaca ccgcctccac tcacgcccaa acccgtaaag
      721 atcggtcttg cactgggtgg cggtgccgcg cgcggcttcg cccacattgg cgtcatcaag
      781 gtactggaag cccagggcat tgtccccgac atcatcgttg gcaccagtgc cggtgccgtc
      841 gtcggtgcgc tttacgcctc gggacataac ggtttcgatc tgcagaaact ggcccacaag
      901 atggatgaat cgaaaatcag cgattggtcg ctgcccgatc gaggtgtcct caaaggcgaa
      961 gccttgcagc aattcgtgaa tgatgccgtc gcacagcgtc cgctcgaaaa attgaaaaag
     1021 cccttcgccg ccgtggcgac cgatctgaac agcggcgaaa gcattctctt ccgtagcggc
     1081 aataccggca tggccgtgcg cgcctcggcg accgttcccg gcgtcttcaa acccgtaagc
     1141 atcaatggac acgaatacgt cgatggcggg ctgtcgagcc tgatcccggt gcgcggcgcg
     1201 cgttcgatgg gcgccgacgt ggttatcgcc gtcgacatct cggcgcgacc cggcaaccag
     1261 actgtgcgcg gcacgctcga tatcctcctg caaaccttca ccatcatggg gcagaacctc
     1321 gcccgatacg aactgaagga ggccgatatc gtcattcgac cgcaggtcgg caacgtcgga
     1381 tcaaccgatt tcccggcacg ccacgatgcg attctggaag gcgagaaagc ggctcagtcg
     1441 gcactgccgc aaatccgcga ggcgatcaga aaggcatctg gtatctgaac cgtcggcaaa
     1501 caaaaaagct tcactgttcc aatgcttccc aacgttcaag taacgccatc aactcgtcat
     1561 caatttcggt cagcctcaat gaaattcgct gcgcttcctg cggctcggcc tgatagatcg
     1621 tcggatctgc caatcgctgg ttgatcctcg cctgctcctg ctcaagcgaa gtaatgcgcc
     1681 cgggaagttc ggccagctct cgcgtttcct tgtagctcat tttgcggccg ccatccgcct
     1741 tgctcttggc tgactgtacc gcgcccgacg cttcgcgctt tgccaccggc tgtgcggctt
     1801 cttgagcgcg ccgcctggcc tggtagtcgg cccagtcctg atagccgccg gcatattcgt
     1861 gccagacgcc ctcgccttcg aacgcgatgc tttgcgtcac cacgttatcg ataaaagccc
     1921 ggtcgtgact gacgaggaag agcgttcccg tatagtcctg caacaactgc tcgagaagtt
     1981 ccagtgtttc gatgtcgagg tcgttggttg gttcgtcgag taccaacacg ttggccgggc
     2041 gggcaaacag tcgcgccagc agcagccggt tgcgttcgcc accggaaagc gatttcaccg
     2101 gcgaacgggc gcgctgcgga gcaaaaagaa agtcgccaag atagccgatc acatgctttt
     2161 tctcgttgcc gatctcgacg taatccgatc ccggcgagat cacgtccacc agcgccgcct
     2221 cttcgtcgag ctgcgtcctg aactggtcga agtatgccac ttcaatcttt gtacccaggc
     2281 gcacgcggcc cgaatccggt gccagctcgc cgagaatcag gcgcagcagg gtcgtctttc
     2341 ccgcaccatt ggggccgatc aggccgatct tgtcgccgcg ctgcaaacgg caggaaaaat
     2401 cgcgcaccac ggtcagtcgt tgtgcaccct cgccaaaatg cttgctcacg tgctccagtt
     2461 cagcaaccaa cttaccgctc ttgtcgcctg catccagagc cagttcaacc ttgccctgcc
     2521 gttcgcgccg ggctacccgc tcacgtcgaa gctgttcgag acgcagcacg cggccttcgt
     2581 tgcgcgtgcg tcgcgcttcg acgcccttgc gaatccacac ttcttcctgc gccagaaatt
     2641 tgtcgaactt ggcgttctgg atcgcctcgt cgtgcagaat cgattccttc ctggccagat
     2701 aggcgctgaa gtttccgggg caggagagca aacggccacg atccagttcg acgatgcgtg
     2761 tcgatacgcg atccagaaaa cgccggtcat gggtgatgaa caccatcgac acccccgatg
     2821 aaaccaaaaa ctcctcaagc cattcgatgg cgccgatgtc cagatggttg gtcggttcgt
     2881 cgagcagtaa cagttcgggc gaaatggcca gagcctgggc caaggccaga cgcttcttct
     2941 gcccgcccga gagggtgcca acgcgcgcat cgggatcgag cgaaaaacgc tggatcaccc
     3001 gctccgcctg catttcgaaa gaccacgcgt tgagcgactc aagctcgccc tgcaattggt
     3061 gcattcgctc aagtaatgct tcatgatcgg ccgaatcttc ggccatggcg tgcgcaacgg
     3121 cgtggtactc ggcaagcagt gtggaaactt ctcccatccc atccaccact gcctcgaaca
     3181 ccgagcgatc cggatcgaat ggcggctcct gcggcacata gcccctgcgg attccaggtt
     3241 gctgccacac ggtcccgtcg tcaagcgttc cctgcccggc cagtgcacgc aacagcgacg
     3301 atttgccggt gccgtttcgc ccgatcagcg ccacgcgttc accaggatca agctggaaat
     3361 cagcatgatc aagaagggga acatggccat aggccagcga agcatcggac agaatcagct
     3421 gcggcatgag atcagtttac tttccagggg aggataacga ttgcctgagt acggcatttt
     3481 catgaattga acggaatttg tcagcgtagc gcacgggcgc gataaaatac gctccgatct
     3541 cctcgtagca taacggatag tgcacgcgcc tcctaagcgt gagatatggg ttcgattccc
     3601 gtcgaggagg ccaagtatgc gttttaagta gtccggcaac atccaatcag cccgcctaag
     3661 tgcgggtttt attcttttat gcggacgccg tcgaactcct catgtccacc atgcggccga
     3721 ccattgcgcg cctttcagtc actcggcatc agcgggccat ctgaagcgaa accgcaccat
     3781 gacgtggtga attcctgcat cgggtcggaa gcatcggact cggatcctgg ctcgctgtgt
     3841 ttgctcaggt attcaccaag cttcttatcg ctggccagga tgtgatgcat caaccaggtg
     3901 tgcagggatg tggtcaattg actcggactg acctgagcat tggccttgag ctgcaggtca
     3961 tagctggcaa gcgtttccac gaggtccagg tgctcggcat gatgctcgtt ggcccaggga
     4021 tagccatggt cgtgcatcag tttttcctcg tacgaaaaat ggtcgatgac gaaatcgatc
     4081 agcgctttca tcacatttct cgaattggtt ccgtcatcga ctgaacgcgc actgaagtcg
     4141 agccggtcaa ggatgcgcaa tatttccctg tgctgtgagt cgataccggc atggccggtt
     4201 tcgtaggtaa acgaccacgc cagcatcggg ttgctgtctt cttctgcgag tttcttgact
     4261 ctcggcttgg atgagacctg gtagggcggg ttctgcgcga aaccggtcaa ggtttgcaag
     4321 ggtagcgggc gagagaagta gtagccctgg atcacatcgc agccattggc ttcgagaaaa
     4381 gcgacctgtt ccggggattc gacgccttcg gcaacggtca tgatgttgag cttgttgcac
     4441 atctcgagga ttgccagcac gatgggactg ttcttgggtt cgctaatgaa ggactggtcg
     4501 attttcagga aatcgaatgg gagctgatac aggtatgaca atgacgagta acccgtgccg
     4561 aaatcgtcca gcgccaaatt gacgccgatt ttcttcaagg ccagcagggt agtcttggtc
     4621 ttttcaaagt cgttgatgaa ggcgctttcg gtaatctcca gagtcagccg gtagccggcc
     4681 ataccgtcca gcgcttcgat caccttggcg acaatgtcgc tttgagtgag ttgcatggga
     4741 gacaggttga ccgacatgtt gatgccggtg cttctcatga cgcgacaggc ctcgcgcaac
     4801 acccattcgc cgatttcgca gatcagcccg gactcttcgg caatgggtat gaaacgcgcc
     4861 gggctgatga ggcccaggtc cgggtgcgac cagcgcacca acgcctcggc gccgtgcacg
     4921 ctcttgctga cgaggcagag ctgtggctgg tagtgcaata acagtccgtc aggaatcgcc
     4981 ttgcgcagcc cgttctcgac catcagtgtt tccagagcct tggcattgat gcgctcggtg
     5041 aagaactgga agttgtttcg tccctggttc ttggcgtgat acatggcggc gtcggaatga
     5101 ctcaacaggt cggtgacgct gaggccatcc gttggaaaca ggctgatgcc gatcgaaggc
     5161 gtgacggtga cgacatgtcc gccgagacgg cagctactcc cgatcgccgc caatagcttc
     5221 ctggcggtat gggtgacatc tgcctcgtcg ccgatatcct caagaagcag cacgaattca
     5281 tcgcctccct gccgggaaat ggtgtcgctc gcacgcagac acccctggat acgacgggtc
     5341 acttccttga gcagcgcgtc gccgatgtgg tggccaagcg tatcgttcac cgccttgaat
     5401 ttgtcgaggt caatgaacat cacggcgaac ttggaccggt tgcgcaccgc acgcgccagc
     5461 gcctgccgca agcggtcttc cagcaggctg cggttgggca aaccggtcag gaagtcatga
     5521 tgcgccatgt gctccatctt ttccatcacc cgattcgact cggtgatgtc ggatagcaaa
     5581 ccgatatagt tggtcacgtt ggcaccggtt ttcacagcgc tgagatggac gcggacaaga
     5641 tactcctcgc ccgttttttt cttgttccat atctcgccct gccagtagcc aaggcgggcc
     5701 agtgttttcc acatctttcg atagaagctg tcgggaagcc gattggaggc gaacatcttc
     5761 gggttcctgc cgatgatttc atcccggcga tatccggtca ggctttccag ggccttgttg
     5821 accgatatgg cgcggttgtc ggcgcccgtg atgatgatgc cttcaaggct gttctcgaag
     5881 acgctggcgg acaacttcag ttgatcctcg gcctcctggt gatgggtgat gtcctggagc
     5941 acttcgatgg ccccgatgat tctcccgtca tcgaacaccg gccgggcctc gaatgccagg
     6001 taacgccgat tgccgttgat gttgtcgaac cagccttcag ccttgtggcc gtcgctggcg
     6061 aattcggttt gctcgtgccg actgtaatag cgcgaagctt cgtccagcat gctatccaga
     6121 accagatcgg ccatgcacgg ccgcgggtcg tcataaaacc cgcgccagtg atctcgcgta
     6181 ccgacaacat cgcgagcggg gatgccggtc aacaactcgc agggtgcatt ccacaccacg
     6241 accttatgct cggtatcgag cacaaaggcc ggaagactca gagcattcaa tacgctaagc
     6301 gcaattttca cctgaaagac ccctgaatgt gcaaagcgct accaatatgc cgctaccagc
     6361 gaaactcggt gcccaatctc ccacacaatc tgctcgctcg gttcagcgac gacaacctat
     6421 tcggttctcg cccatcccca agaggccatc acttgccttt cgttcctgat gcaccagcgc
     6481 cgcgccaaat catctggcgt gctcagggtg atatcagacc tccgtcttgt aaccaatgcg
     6541 gaaaccgccc cagtgccgcc ccttcacata caccggagcc gagatgtcat gcatgatctc
     6601 tccggtatcg cgacggtatg tctgcaacag gaaaggctgg tcgtgagcgc cgcaacggcg
     6661 gccgaccgga tcatcgaaca tgcgtttggt ccgattgttc acgaaatccg ttttttcgtt
     6721 gccggtcagc ggctgtgaga atttccgatt gtgcgtcgga acataaccct tctggtcgca
     6781 ggcaatggca tagaccaccc atttgttctg ctcgagcaac tgctcctgcg tcggcgacac
     6841 attctggtcg gtgaagtcgt cataacgggt cttgtatttc ggtggcttgg tattggaaat
     6901 cggctggtat ttgtcgtcaa acaaatcctc gagtttgatc cgccccgaat cgacggcctt
     6961 ctcgaatatc tggctgacgg cctgcgccgc gcgctggacc aaatcaggca ttttggcatg
     7021 tgtttcaacc gctttatcac ccgccgggcc aagcttgaaa acgttgccga tctctttcag
     7081 gttcgttgcc aggtaatcga gctggttcgc ttcgccgaga gccttctctg ccgccgcgct
     7141 gttgttctcc gccatttgca tgatgttggt cacatggtcg gcaatggtgt ttcccgccct
     7201 gctttgttgg gcaaccgcag cggcgatggc gtcaactttt tccatcgtca cgcgcgcacc
     7261 gctgttgatg ctctcaaggg acgccgccgc ttgctgggca agatcggcac cattttttgc
     7321 ctgtcccgta ccggcctcga tactggtgat cgcgctctgc gtctcgccct ggatcgcccc
     7381 gatcatctgg ctgatttcgc cggtggcttt ggccgtccgt tcggcgagtt tgcggacttc
     7441 gtcagcgacg acggcaaagc ctcgcccctg ctcacccgca cgggccgctt cgatggctgc
     7501 attgagcgcc agcaggttgg tctgatcggc aatctcgcgg atggtctgaa caatgccgct
     7561 gatcgccttc gaacgctcgc caagtgcata caccacctgc gccgactggg tgacggacga
     7621 cgaaatgcgc tccatttcag cagacgcgtt atgtacgatc gtcatgccct ctgccgaaag
     7681 attgctggag gtttcggaga tgccggcggt ctcggttgca ttctggctga cctggttcat
     7741 gttttcggtc aactgggtca tcgcgccgga ggtcgccagg gcggcatcgc gctgcttgtt
     7801 ggacccggat gcgacgcgat tggcttcggc gatcaacttc tgagaagcat gcgcgacttc
     7861 gaccgagttg aaaaacacct tgccgatgat cgtctggatg ctctccatca gcttgttgaa
     7921 ttcagcagca gtctgggaga cttcgtcgcc accttcaacc tgagcgcgtc gggtcaggtc
     7981 gccatccata tacatttgcc cgattactcc gcgcaacgaa ttcaagcgtc ggaccaggcc
     8041 gctttcgatg agcaaactga gaacgacggc gaccacgacc agcaggccat aggcaatgct
     8101 ccagccctgc attccgccac cgaacacgtt caccgccagc caggctgcca acccggcaca
     8161 ggccaccagc aacagcatcg tgccacgaag cttccaagat acgccattca tttgtcacct
     8221 cccgaattgg tatatttttc aatacttcaa ggcaagtcta aagccgatca agcctgcacc
     8281 tgcgaactaa ttctgaaaga ctctcgcaaa ctatccggca cggtgatgca accccgtcac
     8341 aattccgtcc tgtttgcaag caattccatg attaccgtgc gtaatcagca caatgtacgt
     8401 cccgcaatgc acgtccatct gccggctgag aagctatccc aattatcgac agaatacagt
     8461 cgaaccccgg agagtatcgc taaacttggc tggtgtggtc aatctgaaat ttcaaaaaat
     8521 gcccgtttgc gggaaccgcg agtgcttcca tccgatcaaa tgccgttaaa atcagcaccg
     8581 cacattggat agaggagagt gggatggaca tgcaactggc tgaatggtcg gataaattgg
     8641 agttcggcct gcccaggata gacagccagc acaaacagct tttcgacctc gcggccacct
     8701 ttgccggcaa tggggaccag attcgggtga tgaagtctct ggtcatgctg agcaactatg
     8761 cgaaggtcca tttgcgcgac gaggaagaga tgctggcgtc actcaagtac ccggagctgg
     8821 aagcgcacaa gcggctgcat gcggaattca aggacatgtt gatcaaattg ctcgataacg
     8881 ccaggcaact gtctctcgac gagattgcca aagaagttca atacctcatc tacggatggt
     8941 tctacaacca catcatgcaa gcggatgtcg agtacgtccc ttgggtccgc acgcattcac
     9001 tgaaggcgct cggctgaatg atgcctgcag ggccttttcc ggtttcaggg gaaaagggat
     9061 agcgcacagt tcttgagcaa gggcattccg gctggctgca acaggtgtcg gcgaggatta
     9121 cttggactcc ggaccgaccc tccacgcccc atggccgcta taatgctgtc cttttccgca
     9181 tcacctcacc tcttcctact gcttgtcacc acccgtgcct caattctcgc ccgtatccgg
     9241 ctctccggca gcccggttaa cggtgccctt gccttcattc aaaccgggcg agcgcacgca
     9301 gttgccaccc ttttcgggct cggcggatgc gttgcacatc gcgcaactgg cggaccaggc
     9361 tggacaaagc aggctgttgg ccgtactgac cgccagcgct ggagatgccc agcgcctgct
     9421 tgaggaaatc ccatggttcg cgcccgagct gcgcgtgcgc ttgcttcccg attgggaaac
     9481 actgccctac gacagcttct cgccgcatca tgacctcgta tcggaacgac tggccacgct
     9541 ctacggcgtc atgcggaacg aatgcgatgt cctgttggtt ccggcgtcga cggcggtcta
     9601 ccggctgatg ccgccgtcgt acctggcggc gtacactttt tttctgaaga aaggcaagcg
     9661 actcgactcc gagcagtttc gctcccagct cacgctggcc ggttacgcgc acgtcgctca
     9721 ggtcgttgct cctggtgagt actcggtacg cggcgggcta gtcgaccttt tcccgatggg
     9781 ttcggcgcta cccttccggc ttgacctgtt cgacgatgag atcgagaaca tcaagacttt
     9841 cgatatcgac acccagcgca ccctataccc ggtgcaggaa atccgcctgc ttccggcacg
     9901 cgaattcccg ctcgacgaca agggtcgtac ccgcttccgc cagcgttacc gcgaaatctt
     9961 cgaaggagac ccggcacgat ccggaatcta caaggatgta tcgagcggca tcgcaccggc
    10021 cgggatcgaa tactggctgc cgctgttctt tgaggacacg gcaacactgt tcgactacct
    10081 gcccggcgac acgctgctct gcctgcacaa tgacgttcct gaagcgatcc gcggcttctg
    10141 gcgcgatacc cagtcgcgcc atgccatgtt gtcgggagaa cgtagccggc cgctgttgcc
    10201 gccgggtgac ctgtttctca gcgaagaagc ctttttcgtt gccgccaggc cgcatgcgcg
    10261 agttattttg gccaccggcg ccgacggccc ggccaccgcc ttgccaccag tcgccgtcgc
    10321 ccgccgtgcc gacgatccgt tgagcgctct gaaggctttt atcaccggct ttcccggccg
    10381 cgtgctgctg ctggcagaat cggccggacg ccgcgaaacc ctttccgcac tcttcgccga
    10441 ctatggcctg aaacccgatc cctgctccga tcttggcgaa ttcctcgtcg gcgagagccg
    10501 actggcgctg ggcgtctcgc cgttgcacga cggctttctc ctgacgacga atacaacgag
    10561 tacctcgcag cccgatcaat gggccttcat taccgagacc gaactctacg ccgggagccc
    10621 ctcgcggcgg agtcgtcacg cggcacagcg caaggccacg ctggacaact ggctgcgcga
    10681 cctcaccgaa ctgaaggtcg gtgatccggt agtgcacgaa caacacggca tcgcgcgtta
    10741 tcgaggtctg gtgcaccttg acctgggcga gggcgacatg gagtttctcg aactccacta
    10801 cgccgacgaa gcaaagctgt tcgtaccggt ctcgcaattg catgtcattt cacgttactc
    10861 cgcgtcggac ccggatgccg ctcccttgca cacgctcggc acaccgcagt gggagaaggc
    10921 caaacgtcgc gccgccctgc aggcgcggga cacggcagcg gaactgatgg cgctctacgc
    10981 cctgcgcgct gcgcgccagg gccatgcgtt cgaattcacg gcgcacgact acgacgcctt
    11041 tgccgacggc ttcggctttg aggaaacgcc cgatcaggca gccgccatca acgccgtaat
    11101 cgacgacatg aaatccggtc gtccgatgga tcggctggtt tgcggcgacg tcggcttcgg
    11161 caagaccgaa gtcgctttgc gcgccgcgtt ttgcgccgtg gctggcggca agcaggtcgc
    11221 tgtgctgtgc ccgaccacgc tgctctgcga acagcacttc cagaccttca gcgaccgctt
    11281 cgctcaagtg ggcaacaact ggccgattcg catcgccgaa ctctcgcgct tcaagacggg
    11341 aaaggaatcg gcgcaggcgc tcaaggaact cgccgaaggc aaggtggaca tcgtcatcgg
    11401 aacgcacaag ctgttgcaaa aggacgtcaa gttcagtcgt ctggggcttg tggtcattga
    11461 cgaagaacat cgcttcgggg tgcgccagaa agaggcgctc aaagccttgc gtgcggaagt
    11521 cgacgtactg acgctgaccg ccacgcccat cccgcgttcg ctgggcatgt cgctcgaggg
    11581 cctgcgcgac ttctcggtca tcgctacggc gccacagaag cggctggcga tcaagacctt
    11641 tgtttcaaac ttctccgacg gcatcatccg cgaagcgctg ctgcgcgaat tcaagcgcgg
    11701 cggtcaagtg tacttcctgc acaacgaagt caacaccatc gagaacatgc gcgagaagct
    11761 tgccaagctg gtgcccgagg cacgcatcgt cgtcggccac ggtcagatga gcgaacgcga
    11821 actcgaacgg gtgatgcgcg acttcaccca gcagcgcgtc aaccttctgc tatgcacgac
    11881 gattatcgaa accggtatcg acaacccgca tgccaacacc atcatcatca atcgcgccga
    11941 gaaattcggc ctcgcccagc ttcaccagtt gcgcggtcga gtcggtcgtt cacaccacca
    12001 ggcctacgcc tacctgctca cccatgccgg cctcgacgag agcgccggca aactcgccgg
    12061 cctgaccaaa caggcccgac agcggctgga agccatccag ttgatggacg agcttggcgc
    12121 cggtttctac ctggccatgc acgaccttga aatccggggg gccggcgaag tgcttggcga
    12181 gagccagtcg ggcgagatgc aggaaatcgg tttcaatctg ttcagcgaaa tgctcaagcg
    12241 cgccgtcgcc tcgctcaagc agggtcagga accggacctt tcgcagccgc tcggaatcgc
    12301 caccgaaatc aatctgcaca cgccggcgct gctgcccaac gactatgcgc ccgacgtcaa
    12361 tgaacggctg atactctaca agcgactggc caacggcgaa tcacttgatg acatcaccga
    12421 cctgcatgaa gaactggtcg accgcttcgg agaactcccg ccgcaggcgc aatcgctgct
    12481 cgacagccac cgcctgcgcc tgctctgcaa gccgctcggc atcatcaagc tggacgccac
    12541 gtccgagcag attgttgtgc agttcgaccc ggaaattgcc aaatactcgc cgatcgagcc
    12601 gatccgcatc atcgagctga tccagaatga caaaaactac cggctcgccg gacaggataa
    12661 actggcgctg aaacgccatt gcccgaccct ggccgatcgc gtttccgcag tgaaggatct
    12721 gcttcgccaa ttgaccaaga agtaagtacg gaaagcccag caaatgaaca ccatcgacat
    12781 cgaaaacatc aatgtaagcg ccttcgaccc gatgccctcg cccgaggaca tcctggccga
    12841 cctgccgctg tcgacagagg cggccaggac cgtgatgcga ggccgggaaa ccctgcgcaa
    12901 catcctcgag cgcaaggatc atcgcctgtt tgttgtggtc ggcccctgtt cgatccacga
    12961 tccgctggcc ggcatcgact acgcgcgacg gctgaggaag ctctcggacg aagtcagcga
    13021 aacgctattc ctggtcatgc gcgtctattt cgaaaagccg cgcacgacaa ccggctggaa
    13081 gggatacatc aacgatcccg acatgaatga ttccttcacc gtcgaccagg gaatgaaaag
    13141 agcccgccag ttcctgctcg atgtcgccga agccggcctg gcgacgggca ccgaggcgct
    13201 agacccgatt tcgccgcaat atctcggcga tctgatggcc tggaccgcca tcggcgcgcg
    13261 caccaccgaa tcgcaaacgc accgcgagat tgcctcgggg ctatccacac cggtcggctt
    13321 caagaacgct accagcggcg atgtcgacat cgccatcaac gccatcatct cttcctccag
    13381 accgcacagt ttccttggca tcaacggcca ggggcgaacg tcgatcgtcc gcacgcgcgg
    13441 caaccgttat tgccatgtcg tactgcgcgg cggcgaccgc cggcccaact acgacacggt
    13501 gagcgtacag atgaccgaac aggcgctgat cgcggccaaa ctgcccccca atatcgtcgt
    13561 cgactgttcg cacgccaaca gctacaagaa accggagctg cagccgctgg tcatgaccga
    13621 cgtggtcaat caggttcgcc tgggcaacaa atcgctggtc ggcatgatga tcgaatcgaa
    13681 tctggtcggc ggcaatcagc cgattcccgc cgaccggtcc cagctcaagt acggctgctc
    13741 ggtgaccgat ccctgcatcg actgggacac taccgagaaa accatccgcg aagcggccct
    13801 tcaactgcgt gacatactgc ctaaacgaaa ttcctgaacg aagaatcttc accaccaaga
    13861 caccaagagc acaaaagaac accaaggaat tctatggatt gctctatgct tcatttgctc
    13921 ttgttggtga aacttggtga ctttgtgcct tggtggtgaa gcacttgaac ttagcaacct
    13981 gagccccatc aaggagaacc gattgccttc cacctccccc ctgatccgcc ccttcgccgg
    14041 cctgcgcccg cgcagcgaac tcgccgcggc tgtcgccgcg ccaccgtatg acgtcctctc
    14101 cagcgaggaa gcacgtcagc gcgccgctgg caagccctat tccttcctgc atatttc
//
LOCUS       DAORPM010000025        14049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_863_length_14049_cov_11.001786, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000025 DAORPM010000000
VERSION     DAORPM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14049
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_14049_cov_11.001786"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLD53_02295"
     CDS             <1..647
                     /locus_tag="PLD53_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRC59765.1"
                     /translation="PFERFWMGTDRLTPTGERPPLLLIHGYGCSRAAWWWLRRRLEQA
                     GWAVATISLEPIYTSIENYVAPVSRRVDEVLAATGAHQLVLVGHSMGGLVARAYLRTH
                     GVAKVIRLITLGTPHLGSELAKYGIGENARQMTPGSSWLVALASEEPRVDTLNVYSPH
                     DSYVLPQSNLDFSGSQRRTIDGLGHLSMLYSPRVAMALLDGLEEQKMGSADGGQ"
     gene            667..1380
                     /locus_tag="PLD53_02300"
     CDS             667..1380
                     /locus_tag="PLD53_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59766.1"
                     /translation="MNTLTTSLVLFSLLMVPSAAVVAQGVYVTPGDHGPVFSDKPQSG
                     AKEVTLPPLNIVTPPKAPKRAAPAKAEAKSNGTKTEKPDGAKPEAATVGYRSFSIVSP
                     EDNGSVVANTAIFEVRVVVDPPLRLGDGDAFVVSINGRPVNQRFTATEFMIPPEFWGD
                     TLPPLNQSLQLDASIVDGVGQVLKNAPPIRFFMRHATVTNHRKRPVHLPLVPPQSPDP
                     KPRPALEPTEKARMKAPVK"
     gene            complement(1389..1949)
                     /locus_tag="PLD53_02305"
     CDS             complement(1389..1949)
                     /locus_tag="PLD53_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59767.1"
                     /translation="MDHLIKDSATLELFAWLNPDDELPGWDVLTGSSDGVTLPEDQFT
                     TVQNYFAEYHLEYYRQRMYGDFPSRLHALLFFATRVDADNYRAKHPGRVFGKYLARAV
                     STGPYTVSFHDSSWLDYLRLPHSLNLTTLHEVASYYWRGALVEEIGLSFMNERWREPP
                     VIEALFQGTLEPLHMQQHTPWLPGFS"
     gene            complement(2014..>3180)
                     /locus_tag="PLD53_02310"
     CDS             complement(2014..>3180)
                     /locus_tag="PLD53_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRC59768.1"
                     /translation="AAVLFEEVTSPRGVDSGQGWRRWLDELFLNPRWGLLGSLLVFAL
                     VLLIVFEMSTFLDSLTSARLANWVQQWQPESTAGVVGRAVAEGLIGLVGIVVPYMLPL
                     VLLLVMLEESGVMHRVAFVVDRGFHHIGLHGGVAVPFLIGLGCNVPAISAAATTGSRR
                     ERIVSAILITFVPCSARSAIILAIGGKYLGGLGVFAIFMLTLFVIAALGKLLARRYSD
                     SAPGMVQEIPPFARPVWSNVWRKTWERTEDIVTIVTPLLVVGSVVLALLSHFGADALI
                     NTALIPVTSWWLGLPVVLGVPILFGVLRKELSLLMIYQALGTLEIMPLLNHVQIFTFL
                     IFLTFYVPCLSTFAVMLKTLGRKEAWLSVAISLGCALLMAGAVRLFLELASLVI"
     assembly_gap    3181..3280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3281..3951)
                     /locus_tag="PLD53_02315"
     CDS             complement(<3281..3951)
                     /locus_tag="PLD53_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRC59769.1"
                     /translation="MLQSGTLHTPAVTAIPLRGPLLRGDRRPRVALIGRYGVGKSTIF
                     DAASSPVVRHERLAGVGGAYQECVVDVGLDQISLVDLPSIDSLHHLSEHDQVVLMYLL
                     WGDHWPHVAPHEVVDEHSDFPAPDVLVQVVDATALEKDLELSLELSLFGRPLVIALNR
                     VDEARKKGLYINVQALSERLGAPVIPTIAHMGKGILKLFETVLDVTREKICPLPQPPT
                     KHIAD"
     gene            4279..4968
                     /locus_tag="PLD53_02320"
     CDS             4279..4968
                     /locus_tag="PLD53_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRC59770.1"
                     /translation="MIAHAALLLSVASPLPARVDMPATTINVVMIDSGRGDSRKPVQA
                     PAETPPMMPAKASIPDKSPKPVIRRRAVKQIVVPEKPAALVAEPQAPSITPDPRPYTT
                     PPLNQTAYGGGGGGAAASPAASRAGVDADDMRQYRLSLATAARRFKRYPALARERGWE
                     GATEVALKANALVSLPEVVLVNSSGRSILDEQALEMIRQAVRITMLPEGLKGRDFRIL
                     LPVKFSLDSDQ"
     gene            complement(4962..5504)
                     /locus_tag="PLD53_02325"
     CDS             complement(4962..5504)
                     /locus_tag="PLD53_02325"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRC59771.1"
                     /translation="MNYCNQCGATVELRVPPGDTIPRHICQHCGAIHYLNPKLVIGCV
                     SEWEGDILLCRRAIEPRYGLWTLPAGFMENGESTTQAALRETLEEACARIEVDALFSL
                     VNVPYINQVHLFYRGRLLDTAFASGAESLETTLVPESEIPWEKLAFRSVSLSLKAYFS
                     DRRAGHFEFHEFDLQPLAGY"
     gene            5703..>6151
                     /locus_tag="PLD53_02330"
     CDS             5703..>6151
                     /locus_tag="PLD53_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367039.1"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HRC59772.1"
                     /translation="MFLIVGYVIILASALGTYSVHGSLAALWVPLEYIAIVGLTVGGF
                     VAGNDLKIIKTTVGALPGLFKGSKYNKALYVDLLAMLFEVLAKVRKEGLMSIENDVEN
                     PHESPIFGKYPVLAADHHVMEFITDYLRMMVGGNLNAIEIENLMDVE"
     assembly_gap    6152..6251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6252..6437
                     /locus_tag="PLD53_02335"
     CDS             <6252..6437
                     /locus_tag="PLD53_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055558.1"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HRC59773.1"
                     /translation="LLEQKAQEGSKIYQVVKVVLLASMSGYAPQVAVEFGRKVLFSKD
                     RPTFGELEEELKARKGK"
     gene            6467..7423
                     /gene="motB"
                     /locus_tag="PLD53_02340"
     CDS             6467..7423
                     /gene="motB"
                     /locus_tag="PLD53_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRC59774.1"
                     /translation="MSEDVRPIVIKRIKKVAGGHHGGAWKIAYADFVTAMMAFFLLMW
                     LLGSTANGDLKGIADYFTNPVKVSMQGGSGAGDATSILKGGGKDLTSSAGQVKRGELE
                     STKKTPNLREAKAEQLRKEFEKREKAKLSELKQDIEKLIEGNSMLRQFKNQLLIDVTP
                     EGLRIQIIDEKNRPMFDTSSDELKPYSKVILREIGKALNAVPNKVSFSGHTDASVFGG
                     GGKGFSNWELSANRANAARREMIYGGMDANKVLRVLGLSSSVLFDKNDPLSSANRRIN
                     IVVLNKRTEEAILQEDGNAPIETDSASDTEASALPDRPDAKN"
     gene            7505..8380
                     /locus_tag="PLD53_02345"
     CDS             7505..8380
                     /locus_tag="PLD53_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRC59775.1"
                     /translation="MDERKSASGATTLLREPGVREFAFSAADFERVRKLIYEHAGISL
                     SATKQDMVYSRLARRLRETRLGSFGEYLAMLERGDKTEWEKFVNSLTTNLTSFFREPH
                     HFPILADHLKKLQGNSPIKIWCSAASTGEEPYSIAMTVVETFGGFSVPVSIVASDLDT
                     NVLAIAAKGAYPSDRVDKLSPSRLSRFFLKGSGAQEGSYTVRPELRRLVNFQRLNLLE
                     PNWSVRGPFDALFCRNVMIYFDKPTQYKILSRFAPLMSGKGLLFAGHSESFLHAADLF
                     RSQGRTVYELAPKPG"
     gene            8382..9032
                     /locus_tag="PLD53_02350"
     CDS             8382..9032
                     /locus_tag="PLD53_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HRC59776.1"
                     /translation="MAKDPSILGKVPRPMAEGHKVIFVGASTGGTEAVKSFLLGIPSD
                     CPPILIVQHMPESFTGSFAARLDSLCRPRVIESQGAESLESGTVYVAPGHSHLRIRRG
                     ASGYVTELLKTPPVNRHRPSVDVLFDSAAEVVGHNAIGVILTGMGKDGACGLLRMRKA
                     GARTFGQDETSCVVYGMPREAAVIGAVEEVSGPNDIARRVLAVLGRADRPEGFPGR"
     gene            9341..10312
                     /locus_tag="PLD53_02355"
     CDS             9341..10312
                     /locus_tag="PLD53_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HRC59777.1"
                     /translation="MSELLKNIDARTKLAGTNKLEILLFSLGTNSKTGRSETFGINVF
                     KVREVMRTPVITSAPEMPSSVEGMVSLRGALVPVVDLARYAGVATDTPRSIMIVTEYA
                     GHTQGFLVEGVDTILRLDWSQMRVPPEMLVAQMGGLVTAVTELPDGRLVMMMDVEKVL
                     SETTNYDEEIVFRNIKPLDDSSLTVFFADDSVVARRQITRTLEAMKVKYVGAINGLEA
                     WNELEKIATYAESSGQKVTDLVSLVLTDVEMPEMDGYILTKKIKTDPRFIGVPVIMHS
                     SLSGMSNQQLGKSVGVDEYVPKFEAQKLSETLTRRLLGAEPVVQPNT"
     gene            10345..11283
                     /locus_tag="PLD53_02360"
     CDS             10345..11283
                     /locus_tag="PLD53_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HRC59778.1"
                     /translation="MDLVEKKSLLDGVDARTRLAGSNKMEILLFSLGTRETFGINVFK
                     VREVGRTPHITKTPNMPKGVEGLVSLRGNVIPVLSLVSFLDHKDVPVERGATMMVAEY
                     SKRTLGFLVHEVDRIIRVDWEKVKAPESILSSNQGLITAITELENGTLVSLLDVEQIL
                     ANAFGEALIIDIPPAKVDEEVSVFFVDDSIVARRKISEVFDKLGVKHKHATNGAEGLS
                     RLQGLAAHAQQAGTPLRNDVRLILVDAEMPEMDGYVLTKNIKSDSRFAGIPVVMHSSL
                     SSEANRAMGQAVGVDAYVAKFDAEVLADTLRPMLER"
     gene            11305..11694
                     /gene="cheY"
                     /locus_tag="PLD53_02365"
     CDS             11305..11694
                     /gene="cheY"
                     /locus_tag="PLD53_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HRC59779.1"
                     /translation="MADPKLRILIVDDFSTMRRIVRNLLKELGFTNVDEAEDGAIALQ
                     KLQAGGFEFVVTDWNMPNMDGLQLLQAIRSAPDLKHLPVMMITAEAKKENIIAAAQAG
                     ASGYIVKPFTAATLAEKLQKIFDKMSA"
     gene            11696..12607
                     /gene="cheZ"
                     /locus_tag="PLD53_02370"
     CDS             11696..12607
                     /gene="cheZ"
                     /locus_tag="PLD53_02370"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HRC59780.1"
                     /translation="MSDANSSGDSNELEALFDSIAGGGASASISAAPAPAPKTPAPSA
                     AAPKSSLMQQAREVDGATDDSKDLQALFDSIAAKKSAADNSAAGIEEGAGEESGDWPV
                     QDKVFNQVGQMARQLHDTLGSLGYEKLLEKTASALPDAKDRLAYIANLTEQAACKVLN
                     ATDIANPLQEELEAGAALLSAKWDTLFANNMGVEDFKLLAAETRAFLKTAVPQRTGAT
                     KEQLLEIMMAQDFQDLTGQVIKKVVAVAQDLESQLMQVLVVTMPGEKRTESVNTLLNG
                     PVINAEGRTDVVGSQQQVDSLLDSLGF"
     gene            12618..>14049
                     /locus_tag="PLD53_02375"
     CDS             12618..>14049
                     /locus_tag="PLD53_02375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRC59781.1"
                     /translation="MSDFSGMEDLLQDFLQEAGDLLSDVDNKLVDLERSPDDRRLLND
                     IFRGFHTIKGGAGFLNATELVTLCHLTENLFDQLRNAEMHLTPELMDTIMAATQGVRN
                     MFGELGQAVQPRPAEADVIAALRAALEGEATAEPVAETVVAEAPAAKPEEPAPVANTG
                     KEPDWQVLHGALTGQPVPPAQTAAASPPVTKPEASSPAASAVQIAPHYPPEGRRETDK
                     PGAAVSGATSGRRSEEKAVARESTIRVDTSRLDQVLNLSGEIGLTKNRLTSLRADILA
                     GRNDSETLHALDQAVSQLDLLVSDLQNSVMKTRMQPIGRLFQKYPRIARDLARQLGKE
                     VELALVGEETEVDKTMIEDLADPLIHLVRNAVDHGVESAEERLAAGKPAKSVVRLEAR
                     QEGDHIVLMIADDGRGMNPERIRAKAIEKGIVKEEEANTLDDKQSLNLIFLPGFSTMT
                     QASAVSGRGVGMDVVKTNIQKLNGSVE"
BASE COUNT         3112 a         3612 c         4168 g         2957 t
ORIGIN      
        1 caccctttga acgcttctgg atgggcaccg atcgcctgac gccgacgggc gaacgtccgc
       61 ccttgctgct gattcacggt tacggttgtt ctcgggcggc gtggtggtgg ctgcgtcgtc
      121 gcctcgagca agccggctgg gcggtggcaa cgatcagcct ggaaccgatc tatacgagca
      181 tcgagaatta tgtagctccg gtttcccggc gcgttgacga ggtgctcgct gcgaccggag
      241 cgcatcaact ggttctggtc gggcacagta tgggcggcct ggtggctcgc gcctatctgc
      301 gaacacacgg agtggcgaag gtgatccggc tgatcacgct ggggacgccg catttgggca
      361 gcgaactggc gaaatacggg atcggtgaga atgctcgtca gatgacgccg ggcagcagtt
      421 ggctggtggc gcttgccagc gaggagccac gggtcgatac tttgaacgtt tacagtccgc
      481 atgacagcta cgtgttaccg caatcgaacc ttgatttttc ggggtcgcaa cgccggacga
      541 tcgacgggct gggccacctc tccatgctgt attcgccgag agtggcaatg gctttgctgg
      601 acggtctgga ggaacagaaa atgggcagtg ccgatggtgg gcaatgagca atttcgggag
      661 tcgatcatga atacacttac aacatcattg gttctgtttt ctcttctgat ggttccgagc
      721 gcagcggtag tggcgcaggg cgtctatgtg acccctggtg atcatggccc ggtcttttct
      781 gacaagccac aaagcggcgc aaaagaagtc actttgccgc cgctcaacat tgtcacgccg
      841 ccaaaggcgc ccaagcgcgc cgctcctgcc aaggccgaag caaaatcgaa cggcaccaag
      901 acggaaaaac cggatggcgc gaagccggaa gcggcgacag tcgggtaccg cagcttttcc
      961 attgtgtctc ccgaggacaa cggcagtgtc gtcgccaaca cggccatttt cgaagtgcgg
     1021 gtggtagttg atccgccatt gcgactgggt gacggagatg ctttcgtcgt cagcatcaac
     1081 ggacgtcccg tcaatcagcg atttacggcc accgaattca tgattccgcc cgagttctgg
     1141 ggagatacct tgccgccact gaaccagagc ttgcagcttg acgccagcat tgttgacggt
     1201 gtcggacagg tcctgaaaaa tgcgccgcca atccggttct tcatgcgcca tgccacggtg
     1261 acgaaccacc gtaaacggcc agttcacttg ccacttgtcc cgccgcagtc acccgatccg
     1321 aagcctcgac cggcgcttga accaactgag aaggcgagaa tgaaagcgcc ggtaaaatag
     1381 cttgggggct aggaaaatcc aggcagccag ggcgtatgct gttgcatatg aagtggttcg
     1441 agcgttccct gaaatagcgc ttcgatgacc ggcggttcgc gccagcgttc gttcatgaaa
     1501 ctcaggccga tctcctcgac caaagcaccc ctccagtagt aactggcaac ttcgtgtagc
     1561 gtcgtcaggt tcaggctgtg gggcaggcgc aggtaatcca gccagctcga atcgtgaaag
     1621 gagacggtat agggccctgt gctgacggcg cgggccaggt acttgccgaa cacacggccc
     1681 ggatgcttgg cgcggtagtt gtcggcatca acgcgcgtcg caaagaagag cagtgcgtgc
     1741 agacggctgg gaaagtcgcc gtacatccgc tgccggtagt attcgagatg gtattcggca
     1801 aagtagttct gaacggtggt gaattggtcc tcaggcaggg tcacgccatc agagctgccg
     1861 gtgagaacat cccagccagg caactcgtcg tccggattca gccaggcgaa cagttcaagt
     1921 gttgcggaat ctttgatcaa gtggtccatg gtgatattcg tagtgttcgt ccgctccatt
     1981 ctcacccctc atcggagttt gtccaagggc tgactaaatg acaagactcg ccagttcgag
     2041 gaacaggcga accgcaccgg ccatcagtag cgcacatccg agcgaaattg ccaccgacaa
     2101 ccaggcttcc tttcgtccca gggtctttag cattacggcg aacgtcgata ggcagggcac
     2161 atagaaagtc aggaaaatca gaaacgtgaa tatctgcacg tgattgagca gcggcatgat
     2221 ctccagcgtt ccgagtgcct gatagatcat caacagcgac aattccttgc gcagaacacc
     2281 gaacaggatc ggcacgccga gcacgaccgg cagcccgagc caccacgatg tcaccgggat
     2341 cagcgccgtg ttgatcagtg catcggcgcc aaagtgactg agcagcgcca gaacgacact
     2401 gccgacgacc agcagtggcg tgacaatggt gacaatatcc tcggttcgct cccaggtttt
     2461 gcgccagaca ttgctccaga ccggccgcgc gaagggcggg atttcctgaa ccatacccgg
     2521 cgcgctgtcg gaatagcggc gtgccagcag ctttccgagg gcggcaatca cgaacagggt
     2581 cagcatgaat atcgcgaata cgccgagtcc gcccagatac ttgccgccga tggccaggat
     2641 gatcgccgaa cgtgccgagc aggggacgaa ggtgatcaga atggccgaca cgatgcgttc
     2701 gcgccgcgac cccgtcgttg cggcggcaga aatcgccggc acgttgcagc ccagtccgat
     2761 caggaaggga acggcgacac caccatgcag tccgatgtgg tggaatcctc gatcgacgac
     2821 aaacgcgaca cgatgcatga cgccggattc ttcgagcatg accagcaaca gaaccagcgg
     2881 caacatgtag gggacgacga tgccgaccag accgatcagt ccttcggcca cggcacggcc
     2941 gacgacgcct gccgtcgact cgggttgcca ttgctgtacc cagtttgcca ggcgcgccga
     3001 agtcaatgag tcgagaaacg tgctcatctc gaacacaatc agcaggacga gagcgaagac
     3061 cagcaggctg ccgagcagtc cccaacgcgg gttcaggaac aattcgtcga gccagcgacg
     3121 ccagccctga ccggagtcaa cgccgcgcgg actggtgact tcctcgaaca gtacggccgc
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgtcggcga tatgtttggt
     3301 gggcggctgt ggcaaggggc agatcttttc ccgcgttacg tccagaaccg tctcgaagag
     3361 tttcagtatt cctttgccca tgtgcgcgat ggtcgggatc acgggtgcac ccagccgctc
     3421 gctgagtgcc tgaacgttga tgtacaagcc cttctttcgg gcttcatcga cacggttcag
     3481 agcgatgaca agagggcggc cgaaaaggct caactcaagg ctgagttcga gatccttctc
     3541 cagtgccgta gcgtcgacca cttgaacaag gacatccggt gcggggaaat ccgaatgttc
     3601 gtcgaccact tcgtgcggcg caacgtgtgg ccagtggtct ccccacagca gatacatcag
     3661 aacgacctga tcgtgctcgc tcaaatgatg cagggagtca atggaaggca ggtcgacaag
     3721 cgagatctga tcgagtccga catcgaccac gcattcctgg tatgcgccgc caacgccagc
     3781 cagacgctcg tgacgcacga ccgggctgga tgccgcatcg aagatggtgc tcttgccgac
     3841 tccatagcgg ccgatcagcg caacacgggg acgcctgtcg ccacgcagga gcggaccgcg
     3901 aagcgggatc gccgtcactg caggggtgtg taaggtgccg gactggagca ttgatcaact
     3961 tgaaaactgc attttaaacg acctgcacac cggctgcttg gtatccggat acgatgggag
     4021 tccagctgtc ttgtcaatac gaaggtcgcg gagtgtcttg aagcggaatg tgcggcgaaa
     4081 tgttagactg acggactgta taccaaagcc agtccaccac ccatcgtcgt ccgcacgaac
     4141 cgattcggac tttactgaac ggctacgcag tcgtaggtgg gacagtggtc agccattcca
     4201 acaccctcag cgatttttct gtttctatcg atgttgccct cctcatgccg tacattggtg
     4261 cgggccctga ctgtatccgt gattgcacat gccgcgcttt tgctcagtgt ggcaagccct
     4321 ttgccggcgc gagtggatat gccggcaacg acgatcaacg tcgtgatgat tgacagcggc
     4381 aggggtgatt ccaggaaacc ggtccaagcc ccggccgaaa cgcctccgat gatgccggcc
     4441 aaggcatcga tacccgacaa gtcacccaag cccgtaattc gtcggcgcgc agtcaaacag
     4501 atcgttgttc cagagaaacc tgctgcgctc gttgccgagc cgcaagcccc gtcgatcacg
     4561 ccggatcccc gaccctacac gacgcctcca ctgaaccaga ctgcgtacgg cggcggaggc
     4621 gggggcgcgg cggcttcacc ggcggccagt cgtgccggag tcgatgcaga tgatatgcgc
     4681 cagtatcgtc tctcactggc aacggctgca cgacgcttca agcgctatcc agcgttggcc
     4741 cgggaacggg gttgggaggg agcgaccgag gttgctttaa aggccaatgc attggtgtcg
     4801 ctgccggagg tcgtgctggt taactcaagc ggtcgaagca ttcttgacga gcaggcgctg
     4861 gaaatgatcc ggcaggcggt acgcataacg atgctgcccg aaggattgaa ggggcgagat
     4921 tttcgtattt tgctgccggt gaagttcagt ctggatagcg atcagtaacc ggccagcggt
     4981 tgcagatcga attcgtgaaa ttcgaaatga ccggcacggc gatcagaaaa gtacgccttg
     5041 aggctcagtg aaacgctgcg aaaggcgagt ttttcccacg gtatttcaga ttcggggacg
     5101 agtgttgtct caagactttc cgcgcccgag gcaaaggcgg tgtcaagcag tcgcccgcgg
     5161 tagaacaggt gcacctgatt gatgtacggt acgttgacca gtgagaagag cgcgtcgact
     5221 tcaatgcgcg cgcacgcttc ctcgagtgtt tcgcgcaatg cggcttgtgt tgttgattcg
     5281 ccgttttcca tgaaaccggc aggcagggtc cagagtccat agcgtggttc aatggcacgc
     5341 cgacagagca ggatatcgcc ttcccattcg gaaacacatc cgataacgag cttcgggttc
     5401 agatagtgga tcgctccgca atgctgacaa atatggcgcg gaatcgtatc gccaggcggg
     5461 acacgcaatt caaccgttgc tccgcactgg ttgcagtagt tcatgacagg gagccatcat
     5521 tcatgtctgc gattctatac gatatgcgaa tcgtgcacga tgtgtgccgg gaaaccgcgc
     5581 gtgccaggat gggtttgacg ccggttttta tttgccctgt cgtgccatcc cgctacaata
     5641 tgccgcataa gcgggattca tggccaggtg cggatatgac gtggcatgac agggagaggt
     5701 aaatgtttct aatcgtcggc tacgtcatca ttctggcttc ggcacttgga acctattcgg
     5761 tccatggaag tttggcggct ttgtgggttc cgctcgaata cattgcgatc gttggcttga
     5821 cggtcggtgg tttcgtcgcc ggaaacgacc tcaagatcat caagacgaca gttggcgcac
     5881 tgcccggtct tttcaagggc tcgaaataca acaaggcact ctacgtcgat cttcttgcca
     5941 tgctgttcga ggtgttggcc aaagtgcgca aagagggact gatgtcgatc gagaacgatg
     6001 tcgaaaatcc gcatgaaagc ccgattttcg gtaagtaccc cgttctggcg gcggatcatc
     6061 atgtaatgga attcatcacc gactatttgc ggatgatggt cggcggcaat ctcaacgcca
     6121 tcgaaatcga gaatctgatg gatgtcgaaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nttgctggag cagaaggcac aggaaggttc gaaaatctat caggtggtca
     6301 aggttgttct gctggcctca atgtcgggct acgcgccaca ggtggcggtc gagttcgggc
     6361 gcaaggttct tttctcgaaa gaccgcccga cattcggcga gcttgaagaa gaactcaagg
     6421 cgcgtaaggg caagtagtcg cattcatgac cgtgcgagga ttctgaatga gcgaagacgt
     6481 acgcccgata gttatcaaac gcatcaagaa ggtggcgggc ggccatcacg gcggggcctg
     6541 gaagatcgcc tacgccgatt tcgtgacggc catgatggcg ttctttctgc tcatgtggct
     6601 gctcggttcg acggcaaatg gggatctcaa gggaattgcc gactatttca ccaacccggt
     6661 caaggtcagc atgcagggcg ggtcgggtgc gggcgacgcg acaagtatac tgaagggtgg
     6721 tggcaaggac ctgaccagca gtgccggtca ggtcaagaga ggcgaacttg aatctacgaa
     6781 gaaaactccg aatctgagag aggcaaaggc tgaacaactg cgcaaggagt tcgaaaagcg
     6841 cgagaaagca aagcttagcg agcttaagca agacatcgag aaactgattg aaggcaattc
     6901 gatgctcagg cagttcaaaa accagttgct gatcgacgtg actccggaag gattgcgcat
     6961 ccagattatc gatgaaaaaa atcggccgat gttcgacacc agcagtgatg agttgaagcc
     7021 ctattcgaag gtgattctgc gggaaatagg caaggccttg aacgccgttc ccaacaaggt
     7081 cagcttttcc gggcataccg atgcgtcggt atttggcggt ggaggcaagg ggttttccaa
     7141 ctgggaatta tcagcgaatc gggcaaatgc ggcgcggcgc gagatgattt acggcgggat
     7201 ggacgccaac aaggtattgc gcgtgcttgg cttgtcctca tccgttttgt ttgataagaa
     7261 cgatcctttg agctcagcca accgccgaat caatatcgtg gttctgaaca agcgcaccga
     7321 agaggccata ttgcaggaag acggaaacgc gccgatcgag acggatagcg caagcgacac
     7381 cgaagcatct gcgttgccgg atagacccga tgccaagaat tgagtcgtag cttgtcagtg
     7441 acggggccgg gcgttgctgt gctttgatac cccggtttgg aagtgaagaa tcgagggttt
     7501 gatgatggac gaacgaaagt cagcatcagg cgcgacaaca ctgctgcgtg agcccggtgt
     7561 gcgcgagttt gctttttctg ctgctgattt cgagcgcgtt cgcaagctga tctacgagca
     7621 tgccgggatt tcgctttcgg ccaccaagca agacatggtc tatagccgtc tggcccgccg
     7681 tttgcgcgaa acccgcctgg gttcttttgg tgagtacctt gccatgctcg agcgcgggga
     7741 taaaacagaa tgggaaaaat tcgttaactc cttgacgacg aatctcacgt cgttttttcg
     7801 tgagccgcat cattttccga ttcttgccga ccatctgaag aagttgcagg gaaactcgcc
     7861 gataaagatc tggtgttctg cggcgtcgac gggcgaggag ccatacagca tcgcgatgac
     7921 tgtggttgaa accttcggcg gtttcagtgt gccggtgtcc attgtagctt ccgatctgga
     7981 taccaatgtt ctggcgattg ccgcgaaagg tgcgtatcca tcggatcgtg tcgacaaact
     8041 gtctccatcg cgattgagcc gcttttttct caaaggatcg ggcgctcagg aggggtcgta
     8101 tacggtgcga ccggaattgc gtcgattggt caactttcag cgcctgaatt tgctggagcc
     8161 gaattggtcg gtgcgtggcc cgttcgatgc tctcttttgt cgcaacgtca tgatctattt
     8221 cgataagcca acccaataca agatattgtc acgattcgct cccctaatga gcggcaaggg
     8281 gcttctattt gccgggcatt ccgaaagctt tctgcatgca gccgatttgt tccgctcgca
     8341 gggaagaacg gtctatgaac ttgcgccaaa gcccggctag tatggccaaa gaccccagca
     8401 tcctgggcaa ggtgccacgg ccaatggcgg aaggccataa agttatcttc gtcggggcat
     8461 caaccggtgg aacggaagcg gtcaagagct tcttgctcgg gataccgtcg gattgcccgc
     8521 cgatcctcat tgtgcagcac atgccggaat cgttcacggg ttcgtttgcc gcccgtctcg
     8581 acagcctgtg tcgcccgcga gtcatcgagt cgcaaggcgc tgagtcgctt gaatcaggca
     8641 ccgtttatgt tgcgccgggc cattcgcact tgagaatcag acgtggtgcc tccggctatg
     8701 tcactgagtt gttaaagacg ccgcccgtca atcgtcatcg tccctcggtg gatgtgttgt
     8761 tcgactctgc cgccgaggta gtcgggcaca acgctattgg cgtaattctc actggcatgg
     8821 gcaaagacgg cgcgtgcggt ttgctgcgca tgcgaaaggc cggtgcgcgt acttttgggc
     8881 aggatgaaac gagctgtgtc gtctatggga tgccacgcga agcagccgtg attggcgctg
     8941 tcgaagaagt gtccgggccg aacgacatcg cccggcgcgt gctcgcagtt cttggcaggg
     9001 cagatagacc cgaaggtttt cctggcaggt agattttgcg tctctgaatg caggatcaac
     9061 aagccattcc gctgtcggca ggtatgttct gcgtgggccc ggagccgctt gggccatcga
     9121 atagtatggc gtgcagtcat aaaggaaaga acttctatgg cacagcactt gtttccttgg
     9181 tgaaatgatg ctgaaatggg ccttcagtaa caacaagtgc taggtttgac gcggtattgc
     9241 gcccttttat ttataccgat gattatggtg aaatgacatt tgttcgatta agatgtgctt
     9301 gcatagctat aggggcattt acccaagagg gagttgaggc atgtcagagc tgctaaaaaa
     9361 catagatgct cgtaccaaac tcgctggcac caacaagctc gagattttgc tgttttcgct
     9421 cggcacgaat tcgaaaaccg gacggagtga aacgttcggt atcaatgtct ttaaagtgcg
     9481 tgaagtgatg cgtaccccgg tgattacttc agcaccggag atgccgtcgt cggtcgaggg
     9541 aatggtcagc cttcgaggcg cactggttcc ggttgtcgac ttggcccgct atgccggtgt
     9601 tgctaccgat acacctcgct cgatcatgat cgttacggag tacgctggac acacgcaagg
     9661 ttttctggtc gagggggtag acaccattct tcgcctggac tggagccaga tgagggttcc
     9721 tccggagatg ctggtagctc agatgggtgg cttggttaca gcggttaccg agttgcctga
     9781 tggccgcctc gtgatgatga tggatgtgga aaaggtactg tcggagacaa caaattacga
     9841 tgaggaaatc gtattccgca atatcaaacc gctcgatgat tcatcgctta cggttttttt
     9901 tgcggacgac tcggtcgttg ctcgccgaca gattactcgc acgctcgagg cgatgaaagt
     9961 caagtacgtc ggcgcaatca atggcctcga agcctggaat gaactcgaga agattgccac
    10021 ctatgcagaa tcttccggcc agaaagtgac cgatctcgtt agccttgttt tgactgatgt
    10081 cgagatgccg gagatggacg ggtatatcct gaccaagaag ataaagaccg atccgcgttt
    10141 tattggagtg ccggtgatca tgcactcgtc tctgtccggg atgtcgaatc agcaactcgg
    10201 gaagtcggtt ggcgttgatg agtatgtgcc caagtttgaa gcacagaaac tctctgaaac
    10261 gcttacgagg cgcttgttgg gagctgaacc tgtcgtgcaa cccaatacct gaagcagtga
    10321 gctcgttgga gtaagacgat gacgatggac ttggtggaaa agaaaagcct gcttgatggc
    10381 gttgatgcgc gaacccggtt ggcaggctcc aacaagatgg agattttgct gttttcgctg
    10441 ggaacacgcg agaccttcgg gatcaatgtg ttcaaggtgc gcgaagtggg gcgcacgccg
    10501 catataacca aaacgccaaa catgcccaag ggtgttgaag gtttggtttc cctgcgtgga
    10561 aacgttattc ccgtgctttc cctggtcagt tttctggacc ataaggatgt tcccgtcgag
    10621 cgtggcgcga ccatgatggt tgccgagtat tcaaagcgca cgctcggctt cctggttcac
    10681 gaggtcgatc gcatcatccg tgtggactgg gaaaaagtaa aagcaccgga aagcatcctc
    10741 tcctcgaatc aggggctcat cacggcgatc accgagcttg agaacggcac tctggtttca
    10801 cttcttgacg tcgagcagat cctcgccaat gcgtttggcg aggcgctcat catcgatata
    10861 ccaccggcga aggtggacga agaagtcagc gtattctttg tagatgactc catcgttgca
    10921 cgccgcaaga tttccgaggt cttcgacaag cttggtgtga agcacaagca cgccaccaat
    10981 ggcgcggaag gattgagtcg cttgcaggga ttggcggctc acgcgcagca agccgggacg
    11041 ccattgcgca atgacgttcg gctgatcctc gtggacgcag aaatgcccga gatggacggc
    11101 tatgtcctca caaagaatat taagagtgat tcacgttttg ccggcatccc ggtagtcatg
    11161 cactcatcgc tttcttcaga ggccaataga gcgatggggc aggccgtagg cgtggacgct
    11221 tacgtggcaa agtttgatgc agaagttctg gccgataccc tgcgccccat gcttgaacga
    11281 taagaagtta ttcaggagta aggaatggca gatccaaaat taagaattct aatcgtcgac
    11341 gatttttcca cgatgcggcg cattgtcagg aacctgctca aggagctggg ctttaccaat
    11401 gttgacgagg ccgaggatgg cgcgattgcg ctccagaagc tccaggccgg tggtttcgag
    11461 tttgtcgtga cggactggaa tatgccgaat atggatggcc tgcaactgct gcaggcgata
    11521 cgttccgccc cggacctgaa gcatttgccc gtaatgatga ttacggcaga ggccaagaag
    11581 gaaaatatta ttgctgctgc tcaggcgggt gccagtggct atatcgtaaa gccttttact
    11641 gcggcgacgc tggctgaaaa gctgcagaaa atcttcgaca agatgagcgc ataagatgag
    11701 cgacgcaaat tcatccgggg attccaacga gcttgaagcc ttgtttgaca gtatcgccgg
    11761 cggcggtgcg agtgcgtcaa tttcggcagc acccgctccc gctcccaaaa ctccggcgcc
    11821 ttctgcagcg gcacctaagt cttcgttgat gcagcaagcg cgtgaggttg acggtgccac
    11881 cgacgactcg aaggatttgc aggccttgtt tgattcgatc gctgccaaga agtctgccgc
    11941 cgacaacagc gcagccggca ttgaagaagg tgcaggagag gaatcgggcg attggccggt
    12001 tcaggataag gtgttcaacc aagttggcca aatggccagg cagttgcatg acacgttggg
    12061 tagtctgggt tacgagaagc ttctcgagaa gaccgcatca gcgttgccgg atgccaagga
    12121 ccgactggct tacatcgcca accttacgga acaggccgcg tgcaaggttc tgaatgccac
    12181 cgatattgcc aatccgctgc aggaagaatt ggaggctgga gccgccttgc tcagtgcgaa
    12241 atgggacacg ctttttgcca acaacatggg tgttgaggat ttcaagcttc ttgctgctga
    12301 aacacgagct tttctcaaga ccgctgtgcc gcagaggact ggagcgacca aagagcaact
    12361 gctagaaatc atgatggcgc aagactttca ggatctgact ggccaggtta tcaagaaagt
    12421 tgttgcggtt gcgcaggatc tggagtcgca gttgatgcag gtgctcgttg taacgatgcc
    12481 tggagaaaag cgcaccgagt ctgtgaatac tttgcttaac ggcccggtaa tcaatgccga
    12541 agggcgtact gatgtcgtcg gaagtcagca gcaggtcgat agcctgcttg acagcctagg
    12601 attctagggg gttcagcatg agtgattttt ccggaatgga agacctgctg caggattttt
    12661 tgcaggaggc cggtgacctt ctttcagatg ttgacaacaa actggttgat cttgaaagga
    12721 gtccggatga tcggcgattg ctgaatgata tctttcgcgg atttcacacg atcaagggtg
    12781 gagcgggttt ccttaacgct acggaactgg tgacgctttg tcacttgacc gagaatctct
    12841 ttgatcagtt gcgcaatgcc gagatgcacc tgacgccgga gttgatggat accatcatgg
    12901 cggccactca gggtgttcgc aacatgtttg gcgagctcgg tcaagcggtg cagccccggc
    12961 cggcggaagc ggatgtgatt gctgccttac gcgctgcgct tgaaggggag gctaccgccg
    13021 agccggtagc tgaaactgtg gtagcggaag ctcccgccgc caagcctgaa gagccggcgc
    13081 ccgtggccaa taccggcaag gaacctgatt ggcaggtgtt acacggcgcg ctgactggtc
    13141 agccggttcc tcccgctcag actgcagccg cttctcctcc ggtgacgaaa cctgaagcga
    13201 gcagtccagc agcgtccgcg gttcagattg caccccacta tccaccggaa gggcggcgag
    13261 agactgacaa gcccggcgct gccgtctcgg gcgcgacttc gggtcggcgc agcgaagaga
    13321 aagcggtcgc gcgcgaatcg acaattcgag tagatacttc tcgactcgat caggtactta
    13381 atctttccgg cgagattggt ctgaccaaga atcgtctgac cagtctgcgc gcagacattc
    13441 ttgctggccg gaatgattca gagaccttgc atgcgctcga tcaggcggta agccagctcg
    13501 atctgctcgt ctccgatcta caaaattccg tgatgaaaac acggatgcag cccatcggtc
    13561 gtctgttcca gaagtatcca cggattgctc gcgacctggc acgacaattg ggcaaggaag
    13621 ttgagctggc gcttgtcggc gaggaaaccg aggtcgataa gacgatgatc gaggatttgg
    13681 ccgatccatt gattcacctc gttcgcaacg cggttgatca tggcgttgaa tcggcagaag
    13741 agcgtctggc tgccggcaag ccggccaaga gcgtcgtacg gctcgaggca cgccaggaag
    13801 gcgatcatat cgtcctgatg attgcagatg atgggcgcgg aatgaatccc gagcgcattc
    13861 gggccaaagc gattgaaaag gggatcgtca aagaggaaga agccaatacg cttgacgaca
    13921 agcagagcct caatttgatt ttccttcccg gcttttcgac gatgacgcag gcgtcggcgg
    13981 tctcggggcg cggcgtcggc atggatgtgg tcaaaaccaa tatccagaaa ctgaacggtt
    14041 cggtcgaga
//
LOCUS       DAORPM010000026        13891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_883_length_13891_cov_13.936253, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000026 DAORPM010000000
VERSION     DAORPM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 13891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13891
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_883_length_13891_cov_13.936253"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..348
                     /gene="traA"
                     /locus_tag="PLD53_02380"
     CDS             61..348
                     /gene="traA"
                     /locus_tag="PLD53_02380"
                     /inference="COORDINATES: protein motif:HMM:NF041281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HRC59782.1"
                     /translation="MNRSHRYAVVAVALSLLAGGALAGTTGTEFQALYTWVNDVVTGY
                     FGRAVAVAAVGLGAILSIARVNPVPILSGAGFAIFLQYTPTIITGILTATV"
     gene            364..660
                     /gene="traL"
                     /locus_tag="PLD53_02385"
     CDS             364..660
                     /gene="traL"
                     /locus_tag="PLD53_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HRC59783.1"
                     /translation="MGNDENGYIPKSLDAQERFLWWEMDQAVIAIMLISMGVISGSML
                     MGIALGALAAWQYGRMKAGKHPKFAAHALYWWFPSGLFLKLRATPPSDQRYFLG"
     gene            674..1261
                     /gene="traE"
                     /locus_tag="PLD53_02390"
     CDS             674..1261
                     /gene="traE"
                     /locus_tag="PLD53_02390"
                     /inference="COORDINATES: protein motif:HMM:NF017152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HRC59784.1"
                     /translation="MLFRNYLSEQANATQEIRFMRILLAGLLMVILVVAGVVLKLVGN
                     EKTVLVPPEIKRSFWVSGNAVSREYLEDMAYWYAGLALNITPAVSTYQNSLFLKYAAP
                     SEFGRLQMEMGARADFLKKNNTATQFSVRNITLDEKTLRVALSGTLFTWASDKKAGER
                     QATYLVGFKHMNGRLYVSEFKETSEQNPFDSAAGR"
     gene            1224..1907
                     /locus_tag="PLD53_02395"
     CDS             1224..1907
                     /locus_tag="PLD53_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HRC59785.1"
                     /translation="MNKILLTALLAASLSASAAQVLVGKPDDTLSARVSRSEPTLIRI
                     EGHRVRRIFGAEGEFAVTADKEAGTAYLKPTTDKPTLSIFVSDENGRTWKLLLSVTDG
                     PSDSITIKGRSVAGNALTPGKDVARNQIIKRVLLSLQSDADAEMDARATNEIVPLWTE
                     ALFVLTRVIDGPLKGEKYLLTNTSANAMVLDERELYRRQVIAISIAKPQLSPGETTEV
                     FVISENPGE"
     gene            1900..3177
                     /locus_tag="PLD53_02400"
     CDS             1900..3177
                     /locus_tag="PLD53_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/VirB10 family protein"
                     /protein_id="HRC59786.1"
                     /translation="MSDPAAASGGLTSYLKGLSPKARQYLVLGGLGAGFIGLVFGSIA
                     IWDNQPAPMPQSTRLDKASRNIVTPGAQVDPRDVWMAQSSQQMKEMDNVIQGLKQKMG
                     EVEQKQSAPQPSTRQASVLPPLPPMLPGPGPSSTPQMSAQGPTPLPSGTPVTPLPPLP
                     PPVIPREPGIASFEVSDAIPVAPQGPADAKQGKTYIPSGSFMRVALMGGLDAPTGGQA
                     QNNPWPILLRVQDNAFLPNRYRARVKECFMLGSGYGDISSERAYLRLESLSCVLNNGE
                     VVDATIKGYVVGEDGKAGLRGRLVSKQGQVLANALMTGIIAGIGQGFQQSATSYSTSA
                     LGSVGTIESGKQIQAGIGTGVGKALDRLSQYYITLAEKMFPIIEVDAGRVVDVVLTKG
                     VSLGMAASADEDTYTELWKRGRQIMKKSLNPSE"
     gene            3184..3954
                     /locus_tag="PLD53_02405"
     CDS             3184..3954
                     /locus_tag="PLD53_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HRC59787.1"
                     /translation="MRKSMRVPLVMVLVLVSAASVVSAASPAGETEMEARLKELYPST
                     RISAVRPSEVSGLYEVTMGRNVAFTDPTGRYFVFGHLFDMKEQKDLTAQRLSDINRID
                     FAQLPLQDAIKTVRGDGSRKLAVFSDPDCPYCKGLEGELAKLDNVTVYTFLYPLDGLH
                     PEAKGKSERIWCASNRAKAWSEFMTTGKLPNGPKCATPIERINQLAGSLGINGTPTLI
                     LQDGSLIPGAASAAEIERRLAGKAAPIKAAVTPRLKGD"
     gene            3957..4547
                     /gene="traV"
                     /locus_tag="PLD53_02410"
     CDS             3957..4547
                     /gene="traV"
                     /locus_tag="PLD53_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HRC59788.1"
                     /translation="MRSVCLIASVLALALGGCAGTLTGMDGESKFSCKAPDGVTCSSL
                     SGVYANAVANNLPGLRKDGKGDLAVRPPAKESPEGTITGQVASSGDPLRTQAKVLRIW
                     IAPWEDTEGDLHDQSYIYVVANAGRWAIEHSQRHIVDRYRPTFIKAGPGGQATQKPLT
                     PTQKPSRPLGGPSLPGNQAIGPGGTGGTGTPAEDRE"
     gene            4550..7117
                     /gene="traC"
                     /locus_tag="PLD53_02415"
     CDS             4550..7117
                     /gene="traC"
                     /locus_tag="PLD53_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HRC59789.1"
                     /translation="MLQALKSVFYGERFEPADAVQRSLLDRIATLPRLTGILPYLGWM
                     ADEGMFVLDQGTFGARPTHSMGFCIETVPQTGSNEEMERVLASLFMSCPPGTGIQISL
                     YASPHILPSLREQANMLPVDEEPAGSEWESRRNRNIYRVLGRRRIDHYLKGAAKSLFS
                     HHVYLLRDYRCVIAISTPLDPESPADVEEAIRIKESTHATLRSAHMPGWDWGPESLLN
                     FVADFFDHERVLGQPEERHIEYDSSRTLRSQVSNPEIASAVADSFIKYRKAGGAETAM
                     QCFSVRQYPKYFRMGNMGSMIGDYYQMALSIPCPFLITMGAITLDYESARSKALMKAA
                     RATQAAGSYMAHFQPDLQDRKRDWDMVLRAFDNGRNVVQMYHQIVLLSRIEDVSRTEH
                     AVRAVWRARGFDLAKDVYLQHQAFVASMPCTLTPALQKDLQLFGRAGTKTADNAVMTS
                     PLMAEWKGTATPVMTLFGRRGQIMSFDLFDNTGGNYNFAVAALSGSGKSVFVNEMAFR
                     YLGTGTKVWIIDVGRSYRNLSELLDGEFIEFSDERTNTICLNPFSMVVDINNDMEMLL
                     PLVAQMASPRGPLDNYSYSALAAAIKRVWDQKEKSATITDVYDLLRTGRLSDEGEVER
                     DLSRLATALEPYTKYGIYASYFEGEANIQFRKNLVVLELEELKAKKDLQAVVMQLMMY
                     RITQEMYLDRSKRKLVIIDEAWDLMGSGSSANFIEAGYRRARKYGGAFGTITQSVDDY
                     YKNEATRAAITNADWLFLLRQKPESIDRLGKEGKLHVDEWMKRQLSSVSTEHGHYSEI
                     FVHGPMGSGVGRLILDPFSMLLYSTQAEDYQAIKTLTDQGIPVTEAIETIIERRGGA"
     gene            7114..7488
                     /locus_tag="PLD53_02420"
     CDS             7114..7488
                     /locus_tag="PLD53_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59790.1"
                     /translation="MNLNLGRLLVTGLLSVLVSCAIGLMWVRSHPAPRMVRVDVGSLY
                     EEQKKTLSARLKPGMTEEEQKAIFKAATDYGKRVDEALSSAARECQCAVLNTAAIVRL
                     PDSGESGIPDATDRVRQILGQN"
     gene            7520..>8199
                     /gene="traF"
                     /locus_tag="PLD53_02425"
     CDS             7520..>8199
                     /gene="traF"
                     /locus_tag="PLD53_02425"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HRC59791.1"
                     /translation="MMTGCLLVSSASATVISGGIDYPSIWACEEAKFHWYCDAEPEVT
                     PDPTKPGAKAKTSEEAALEELERLRKALEAKRALALMQPSPENLKAYITAQEALMDRA
                     SVFSDVWRRVIWANPDLNYQLRNPVNNAAIQVRDVERTQKERASMKEIAREWGVFFVF
                     RSDCPYCHRMAPALKLLADTYGITVFPISLDGGVLPDFPSPARDNGMARVLDVKQVPY
                     VALGNIKD"
     assembly_gap    8200..8299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8300..9598
                     /locus_tag="PLD53_02430"
     CDS             <8300..9598
                     /locus_tag="PLD53_02430"
                     /inference="COORDINATES: protein motif:HMM:NF017892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HRC59792.1"
                     /translation="GVAAYQGQTMNLYSGGSLYMRTPVKNYQLATVAPPAFKAGCGGI
                     DLFAGSFSFINKEQLTALMRNIANNAVGYAFMMAVKSISPDLADLIQYLQDQISKVNN
                     LNINSCQAAEGVVAAIGSTLTDKTQEKEGKVAGASLNLYSDVFESWDAWKDRSKARQT
                     RQAAISADSRNRETFDPGNVVWRALLRVNGVDDETRQLMMSMTGTIIIVPPGDDESGS
                     STDQKAKWLYKPGAKITFRQFVGDGSAATATLSGLVCDDYAECRNPAYVEDGITASSF
                     AKFVRDKIVAMKQKVESRSAGGQGTLDVALIGASSLPVWKMLSLAANMPGGDFVVENH
                     TQLVAVDIAHSYFTNLAKTLREAIQNEMGKGNADVAEAAKSILMRVTEIEQEGREMLV
                     AEYQKGVQVAQMQRSIQWLDQSLKAGLPTNVFQSMMAFNR"
     gene            9625..12792
                     /locus_tag="PLD53_02435"
     CDS             9625..12792
                     /locus_tag="PLD53_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HRC59793.1"
                     /translation="MNFEVYAYWNIEELTATFNAVAALMGSGDYLGLLRTLALVGILS
                     LALVVLAGKGRMEDFWKWVIMLALFNGMLLVPKTNVIVVDRTGTTPAQVVANVPIGLA
                     AFAHSTSKIGDWMTGAFETVFALPGDLQFRTSGTLFGHRVLQERLAIKSGQPILTSNF
                     YEFYRECITPELASGYIRMNDLMKTNDIWGTLNGLTNPSRLVTLRDTADPTIMQTLGC
                     TAAYTTLTTQLNAEAANQLGLLGNRMYPGMPSALANTAISGSLATSTNYLLGLSATAT
                     DTIKQSIVSNAVIDAQYTIPAQIGDAASAATNLAQAQAVRQTGDAYKAMAKLAEGTMP
                     KARNAIELVQYAVFPIILLLTLVAGHHGGSVLKMYAGSLLWIQLWPPLYAIMNFLMNV
                     QAQKALTSATNGGGMSLESYSYLNNSVVSDQAIAGMLVLSIPAIAAALIKWGDAGLRS
                     VGASFAPRDAEKAAAALAQGNMNMGNASLGNVQHGNYSALQHSTQPSLAAGAGKMASA
                     DSFGSMERTGDAVTVKSGNMTMTGFDRNRDGRFTIDEVTNVSAAGSSLMGAIAQSGLG
                     RSASDVQYSGEGTQASTGKASTIGDSKTAKLTQDQSAVFQRQLSKALNSEIGGDTSAG
                     VTSGAGNAVSTGRDFSAKKGLTNHEGASMDSVLTAGGSGQSKPGEGQASKDHGRAGQL
                     AMALAEKASGVSARVAGGVKTGQRYDESADQTLKTATQDELRQAAEVAKRGLLRVAGS
                     TSDQGVKQAAERAAATLDKAKSFDSKELASLTEQQEAGSRRQAGNENRSNVSVDESLI
                     MAKSGYEMLFGKGSEVTPERLAAFQREWNSNQGFREDVALETRDRLAKSRLAAEGVEP
                     PISQDTVRAEGQTALAALDSKGNDAVASQGRANSRHVERQQYQNPRSMPDLSPAAKTY
                     AESFNAASEQVKRKESQVSLESGITVMANQLYQERQRGTGTVLLNTYGSGAGSASVQD
                     YAGKLREASQKDPELAQGLATIGSNNRAGVQPSHADMAWLEGKAKQSVRQAEGSASEG
                     AGWVADQAGKALDLFSPSSSPSPRK"
     gene            complement(12814..13230)
                     /locus_tag="PLD53_02440"
     CDS             complement(12814..13230)
                     /locus_tag="PLD53_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59794.1"
                     /translation="MERMVLVSVLLVVPGMTLIYLAARLMAVAVKLFRTGHVVESLLL
                     LVAALVAATLVAMFGNEKSILVAALLSWVFLAVAFIRFVLRNGENDPRQQSYFSDPPH
                     DQDPNSLNFSGVGPGFQGFGMYAGGVRIAQDPLDDD"
     gene            13644..>13891
                     /locus_tag="PLD53_02445"
     CDS             13644..>13891
                     /locus_tag="PLD53_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRC59795.1"
                     /translation="MNKSELVEIVAKEADLSKAAAGSALDAVIAAIVKAVSKGDTATL
                     IGFGTFKPSKRAARIGRNPQTGKELKIAATTVPRFTAGA"
BASE COUNT         2965 a         4073 c         4167 g         2586 t
ORIGIN      
        1 ttggcatcgg gctgggctgg ctcatcaaat ctgtcatcga ccataccaaa ggagttaaaa
       61 gtgaatcgta gtcatcgtta tgccgtagtc gccgtcgccc tttccctgct ggccggaggt
      121 gcccttgcag gcaccacggg aactgaattc caggcgctct atacctgggt caacgacgtg
      181 gtcaccggtt atttcgggcg ggcggtagcg gtggcggccg tcggtcttgg cgcgatcctt
      241 tcgattgccc gcgtcaatcc ggttccaatc ctgtcgggcg ctggctttgc gatattcctg
      301 caatacacgc cgacgatcat caccggcatc ctgacggcaa cggtctgagc ctgggcatcc
      361 cgcgtgggta acgacgaaaa cggctacatc ccgaagtccc tggatgcgca ggagcgcttc
      421 ctgtggtggg aaatggatca ggcggtgatc gccatcatgc tgatcagcat gggggtgatc
      481 tccggatcca tgctcatggg catcgccctg ggcgcactgg cggcctggca gtacggtcgc
      541 atgaaggcag gcaagcaccc gaagttcgcc gctcacgccc tctactggtg gtttcccagc
      601 gggctgttcc tgaagttgcg agccactcca ccgtctgacc agcgctactt cctgggctag
      661 gcgacgaatc acgatgctat ttcgaaatta cctgtccgag caggcgaatg ccacacagga
      721 aatccgcttc atgcggattc tcctggcggg actgctcatg gtgattctgg tcgtcgccgg
      781 cgtcgtgctg aaactggtcg gcaatgaaaa aaccgtcctc gttccgcccg agatcaagcg
      841 cagcttctgg gtctccggca acgccgtctc ccgggagtac ctcgaagaca tggcctactg
      901 gtatgccgga ctcgccctca acattacccc ggccgtttca acttaccaga acagcctgtt
      961 cctgaagtat gcggcgccgt ccgagtttgg acgactgcaa atggaaatgg gtgctcgcgc
     1021 ggatttcctt aagaagaaca acaccgccac gcaattctcg gttcgcaaca tcacgctgga
     1081 cgagaagacg ctgcgggtcg ccctgtcggg gacgctgttt acctgggcgt cggacaaaaa
     1141 ggccggggag cgccaggcga cctatctcgt cggattcaag cacatgaatg gaaggctcta
     1201 tgtatccgag ttcaaggaaa ccagtgaaca aaatcctttt gacagcgctg ctggccgcta
     1261 gcctgtccgc cagtgcggct caggtgctcg tcgggaaacc ggacgacacc ttgagcgcca
     1321 gagtatctcg atccgaaccg accctgattc ggatcgaggg acatcgcgtt cgccggatct
     1381 tcggggcgga aggtgagttc gcggtcacgg ccgacaagga agccggtacc gcctatctca
     1441 agccgaccac ggacaagccg accctcagca tcttcgtttc cgatgagaac ggccgaacct
     1501 ggaagctgct gttgtcggtg acggacggcc cgtcggattc catcaccatc aagggacggt
     1561 cagtcgccgg caacgctctc acgccgggca aggatgtcgc cagaaatcag ataatcaagc
     1621 gcgtcctcct ttcccttcaa tcggacgccg acgccgaaat ggatgcgagg gcgaccaacg
     1681 agatcgtgcc cctctggacc gaagccctgt tcgtcctgac cagggtcatc gacggtcccc
     1741 tcaaaggcga gaagtacctt ctcaccaaca cgtccgccaa cgcgatggtg ctcgacgagc
     1801 gggaactcta ccggcgccag gtcatcgcca tttcgatcgc caaaccccag ctgtcgcccg
     1861 gtgaaaccac tgaggttttc gtgatctcgg agaaccccgg tgagtgatcc ggctgccgcc
     1921 agcggcggtc tcacgtccta tctgaaaggc ttgtcgccca aggcccgaca gtatctggtg
     1981 ctcggcggcc tgggggcagg attcatcggc ctggtctttg gcagcatcgc gatatgggac
     2041 aaccagcccg ccccgatgcc tcaatcgacc aggctcgaca aggccagcag aaacattgtc
     2101 acgccgggcg cgcaggtgga tccacgcgat gtctggatgg cgcaatcgtc gcagcagatg
     2161 aaggagatgg acaacgtcat tcagggcttg aaacagaaga tgggtgaggt tgagcaaaag
     2221 cagtctgcac cgcaaccctc gacccgtcag gccagcgtgc tgccgccctt gccgccgatg
     2281 ctcccgggtc ccggcccttc cagcacacct cagatgtcag cccagggccc gacaccattg
     2341 ccgtccggga caccggtaac acctctcccg ccgttgccgc ctcccgtcat tccacgagag
     2401 cctggaattg catcgttcga agtcagtgac gcgattccgg tagcaccgca agggccggcc
     2461 gacgcaaaac agggcaagac ctacatcccc tcgggctcct tcatgcgggt ggcgctgatg
     2521 ggtggactgg atgcgcccac gggaggacag gcgcaaaaca atccgtggcc gatcctgctg
     2581 cgggtgcagg acaacgcctt tcttcccaat cgctaccggg ccagggtcaa ggagtgtttc
     2641 atgctcggtt cgggatacgg cgacatcagt tccgaacggg cgtatctgcg ccttgaatcg
     2701 ctctcctgtg tgctcaataa cggcgaagtg gtggacgcca cgatcaaggg ctacgtcgtc
     2761 ggcgaggacg ggaaagccgg attgcggggg aggctggtca gcaagcaagg acaggtgctg
     2821 gccaatgcct tgatgaccgg aatcatcgcc ggcatcggcc agggattcca gcaaagcgca
     2881 accagctaca gcaccagcgc cctgggctcg gtggggacga tcgaatccgg aaagcagatc
     2941 caggcaggca tcggcaccgg cgtcggcaag gcactggatc ggctctccca gtactacatc
     3001 acgctcgccg agaagatgtt tccgatcatc gaagtggatg ccggccgggt cgtcgacgtg
     3061 gtcctgacca agggcgtgtc gctgggcatg gccgcgagcg ccgatgaaga cacctacacg
     3121 gaactctgga agcgagggcg ccagatcatg aaaaagtccc tgaatccttc ggagtaacaa
     3181 caaatgcgta aatcgatgcg tgtccccctg gtcatggtcc tggttcttgt ttcggcggcg
     3241 agcgtggtca gtgctgccag tcctgccggc gagactgaaa tggaagcgcg cctgaaagag
     3301 ctttacccct cgacccggat cagcgccgtt cgtccctcgg aggtctcggg cctctatgaa
     3361 gtcaccatgg gaagaaacgt ggccttcacg gacccgacgg ggcgctactt tgtcttcggc
     3421 cacctgttcg acatgaagga gcaaaaggat ctgaccgcac agcgactgag cgacatcaat
     3481 cgcatcgatt ttgcccagtt gccattgcag gacgcgatca agaccgtacg cggggatggc
     3541 agccgcaagc tggcggtgtt ctctgatccg gactgtccct attgcaaggg gcttgaaggc
     3601 gaactcgcca aactcgacaa cgtcacggtc tacacctttt tgtatccgct tgacggtctg
     3661 catccagaag ccaaggggaa gtctgaacgc atctggtgcg catccaaccg cgcgaaggcg
     3721 tggtcggagt tcatgaccac gggcaagctg cccaatggcc ccaagtgcgc cacgccgatc
     3781 gagcgcatca accagctggc gggcagtctc ggcatcaacg gcacgcccac cctcatcctg
     3841 caggacggga gtctgatacc gggagcggcc tccgccgctg aaatcgagcg tcgactggcg
     3901 ggcaaggcgg caccgataaa agccgctgtc acgcccagac tcaaggggga ttgatcatgc
     3961 gttcggtctg cctgatcgca tccgttctcg ccctggcgct gggcggctgt gccggaaccc
     4021 tgacgggaat ggatggcgag agcaagtttt catgcaaggc gcccgatggc gtgacctgtt
     4081 cctccctgtc cggggtttat gccaatgcgg ttgccaacaa tcttccgggg ctgcgcaagg
     4141 acgggaaggg cgacctggcg gttcggccgc cggcgaaaga aagtcctgaa ggcacaatta
     4201 ccggtcaggt ggcgtcatcc ggcgacccgc ttcggacgca agccaaggtt ttgcgaatct
     4261 ggatcgcccc ctgggaagac accgaaggcg acctgcacga tcagtcctac atttacgtcg
     4321 tcgccaacgc aggccggtgg gcgatcgagc acagtcagcg acacatcgtt gaccgctatc
     4381 ggcccacgtt catcaaggcc ggtcccggcg gacaagccac gcaaaagccg ctcaccccaa
     4441 cgcaaaagcc gtcccggccg ttgggcggac cctcccttcc gggaaatcag gccatcggtc
     4501 cgggcggcac gggcggtacg ggcacaccgg cagaggaccg cgagtgagcg tgctgcaagc
     4561 cctcaaatcg gtcttctatg gcgagcgatt cgagcccgcc gatgcggttc agcgcagcct
     4621 gctggatcgc atcgccacgc tgccaagact caccggcatc ctgccttacc tggggtggat
     4681 ggcagacgag gggatgtttg tgctcgacca gggcacattc ggcgccaggc ccacacattc
     4741 catgggcttt tgcatcgaga cagtcccgca aaccggatcc aatgaagaaa tggaacgggt
     4801 gctggccagt ctgttcatgt cctgtccacc gggtacgggc atccagattt ccctgtacgc
     4861 cagtccgcat attctgccgt cgctgcgaga gcaggccaac atgctcccgg tggatgagga
     4921 gcctgctgga tcggaatggg agtcccgtcg caaccggaac atctatcgag tgctcggccg
     4981 gcgccggatc gatcactatt tgaagggcgc ggccaaatcg ctcttctcgc atcacgtcta
     5041 tctgcttcgc gactaccgct gcgtcattgc catcagcacg ccgctggatc ccgagagtcc
     5101 cgccgatgtg gaggaggcga tccggatcaa ggagagtacc cacgccacct tgcgctcggc
     5161 ccacatgccg ggctgggact gggggccgga aagcctgctg aactttgtcg cggacttctt
     5221 tgaccacgag cgggtgcttg gccagcctga agagagacac atcgagtacg acagttcgcg
     5281 cacccttcgc agtcaagtgt cgaatccgga aattgcttcc gccgtcgccg attccttcat
     5341 caagtatcga aaggcgggcg gtgccgagac cgcgatgcaa tgcttctccg tgcggcagta
     5401 cccgaagtat ttccggatgg gcaacatggg ctcgatgatt ggcgactact accagatggc
     5461 cctctcgata ccgtgtccgt ttctcatcac catgggggcc atcaccctgg attacgagtc
     5521 ggccagaagc aaggcactga tgaaggcggc gcgagcgacc caggccgccg gctcctacat
     5581 ggcccatttc cagccagacc ttcaggatcg caagcgcgac tgggacatgg tgctgcgcgc
     5641 cttcgacaac ggccgcaacg tggtccagat gtatcaccag atcgtcctgc tctcccgaat
     5701 cgaggacgta tcccggaccg aacatgccgt ccgtgccgtc tggcgcgcgc gcggtttcga
     5761 tctggccaag gatgtctatc tgcagcatca ggcatttgtg gcgtcgatgc cgtgcaccct
     5821 gaccccggca ttgcagaagg acctccagtt gttcggccgt gccggcacca agaccgccga
     5881 caacgcggtg atgacgtccc ccctgatggc ggaatggaag ggcaccgcga ccccggtcat
     5941 gaccctgttc ggtcgtcgcg ggcaaatcat gagcttcgat ctcttcgaca acacgggcgg
     6001 caactacaac tttgccgtcg cggcgctgtc cggttccggc aagtcggtct tcgtgaacga
     6061 aatggccttc cgctatctgg gtaccgggac caaggtctgg atcattgacg tcggtcgctc
     6121 ctaccgcaac ctgtccgagc ttctggacgg ggaattcatc gagttctccg atgagcgaac
     6181 caacacgatc tgtctcaatc cgttttccat ggtcgtcgat atcaacaacg acatggaaat
     6241 gctgctgccc ctggtggccc agatggccag tccccgcggc ccgctggata actacagcta
     6301 ctccgccctc gccgccgcga tcaagcgcgt gtgggatcag aaggaaaaat ccgcgacgat
     6361 cacggatgtc tatgacctgc tgcgcaccgg gcggctgagt gacgaaggcg aggtcgagcg
     6421 agacctgtcc cgactggcga ccgccctgga gccctataca aagtacggca tctacgcgag
     6481 ctacttcgag ggggaggcca acatccagtt tcgcaagaac ctggtcgtgc ttgaactgga
     6541 ggaactcaag gcgaagaagg atctccaggc ggtcgtcatg cagttgatga tgtaccggat
     6601 cacccaggag atgtatctcg atcgatccaa gcgcaagctg gtcatcatcg atgaggcctg
     6661 ggatctgatg ggcagcggat cgagtgccaa cttcatcgag gccggctacc ggcgagcacg
     6721 caaatatggc ggcgcgtttg gcaccatcac gcaatccgtg gatgactatt acaagaacga
     6781 ggcgacgcgg gcggccatca ccaatgccga ctggttgttc cttctcaggc aaaagccgga
     6841 aagcatcgat cggctcggga aggagggcaa gctccatgtg gacgagtgga tgaaacggca
     6901 attgtcttcg gtcagcacgg agcatggtca ctattcggag atcttcgtgc atggcccgat
     6961 gggctccgga gttggacgcc tgattctcga tcccttctcc atgctgctgt actcgacgca
     7021 agccgaggac tatcaggcca tcaagacgct caccgatcag ggcatccccg tcaccgaggc
     7081 cattgagacc atcatcgagc gccggggggg cgcatgaatc tcaaccttgg tcgattgctc
     7141 gtcacgggtc tcctgtccgt cctggtgtcg tgtgccatcg gactgatgtg ggttcgttcg
     7201 catcccgctc cacgcatggt gcgggtcgat gtcggcagtc tctacgagga acagaagaag
     7261 acgctcagtg cgcggctcaa gccgggcatg accgaggaag aacaaaaggc gattttcaag
     7321 gcggcgacag actacggaaa gcgtgttgat gaggccttga gcagcgcggc acgggaatgc
     7381 caatgtgctg tcctgaacac ggccgccatt gtccgcctgc cggattccgg tgaatcggga
     7441 attcccgatg ccacggaccg ggttcgacag attctgggcc agaactaggt ccgtgcagat
     7501 gcgcaaggaa tggctggcca tgatgactgg atgccttctg gtgagttctg cgtcggccac
     7561 cgtgatcagc ggtggcatcg actatccgtc gatctgggcc tgtgaggagg ccaagttcca
     7621 ctggtattgc gatgctgagc ctgaggtaac acccgacccg acaaaacccg gtgcaaaggc
     7681 caaaaccagc gaagaagccg cgcttgagga gcttgagcgc ctgcgcaagg cgctggaagc
     7741 caaacgggca ctggccctga tgcagccttc tccggaaaac ctcaaggcct acattacggc
     7801 gcaggaagcc ctgatggatc gggcctcggt gttctcggat gtctggcggc gcgtcatctg
     7861 ggcgaacccg gacctcaatt accagctgcg caatcccgtc aataacgcgg ccatccaggt
     7921 cagagacgtg gagcggacgc aaaaggaacg cgcctccatg aaggagatcg ccagggaatg
     7981 gggcgtgttc ttcgtattcc ggtcggattg tccgtactgc caccgcatgg cgccggccct
     8041 caagttgctg gcggacacct acggcatcac cgtatttcca atcagtctgg atggaggcgt
     8101 cttgcctgac ttcccgagcc cggcgcggga taacggcatg gccagggtgc tcgatgtcaa
     8161 acaggttccc tacgttgcgc tcggcaacat caaggatcan nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnng gtgtggccgc ctaccagggg cagaccatga atctgtactc
     8341 gggcggcagt ctgtacatgc gcacccccgt caagaattac cagcttgcca ccgtagcccc
     8401 tccggccttc aaggctgggt gcggcgggat cgatctgttt gccggttcct tctccttcat
     8461 caacaaggag caactgacgg ccctgatgcg caatatcgcc aacaatgccg tcggctatgc
     8521 cttcatgatg gccgtcaagt cgatctcgcc cgatctggcc gatctgatcc agtacctcca
     8581 ggatcagatc tccaaggtga acaacctcaa catcaattcc tgccaggccg ccgaaggggt
     8641 ggtcgccgcg atcggcagca cgctgaccga caagacgcag gagaaagagg gcaaggtcgc
     8701 cggcgcgtcg ttgaatctgt attccgatgt gttcgagtcc tgggatgcct ggaaggaccg
     8761 ctcaaaggcc agacagacgc gccaggccgc catcagtgcc gactctcgca acagggaaac
     8821 cttcgatccc gggaatgtcg tgtggcgggc gctgcttcgg gttaatggtg tcgatgacga
     8881 aacccggcaa ctgatgatga gcatgacggg aacgatcatc attgttccgc ccggtgacga
     8941 cgaatcgggt tcgagcacgg accagaaagc caagtggttg tacaagcccg gcgcaaagat
     9001 caccttcagg caatttgtgg gcgatgggtc cgcggcgacg gcgacgctat ccggactggt
     9061 ctgcgatgac tacgcggaat gcaggaatcc tgcgtacgtc gaggacggca tcacggcaag
     9121 ttcatttgcg aagttcgttc gcgacaagat cgtcgcgatg aaacagaagg tggagtcgcg
     9181 cagcgcgggc gggcagggca ccctggatgt cgccctgatc ggggcgtcca gcctgccggt
     9241 atggaagatg ctgtcactcg ccgccaacat gcccggcggt gacttcgttg tggaaaacca
     9301 tacccagctc gtggccgtcg atattgccca ctcctatttc accaacctgg ccaagacgct
     9361 gagggaagcg atccagaacg agatgggcaa ggggaacgcg gacgtggcgg aagccgcgaa
     9421 gtcgattctg atgcgggtca ccgagatcga gcaggaaggt cgcgaaatgc tggtcgccga
     9481 atatcagaag ggggtgcagg tggcccagat gcaacgcagc atccagtggc tcgatcagtc
     9541 gctgaaagcc gggctgccga ccaacgtctt tcagtcgatg atggcgttca atcgataggc
     9601 cgatagaacg acgcgctcac accgatgaat ttcgaggtct atgcctactg gaatatcgag
     9661 gaactgacgg ccacgttcaa cgccgtcgcc gccctcatgg ggagcggcga ctacctgggc
     9721 cttctgcgca cgctggcact ggtcggcatt ctctcgctgg cgctggtcgt cctcgccggc
     9781 aaggggcgca tggaggactt ctggaagtgg gtcatcatgc tggccctctt caacggcatg
     9841 ctgctggtcc cgaaaaccaa cgtcatcgtc gtcgatcgca cgggaaccac gcccgctcag
     9901 gtggtggcca atgttccgat tggtctggct gccttcgcgc attcgaccag caagattggc
     9961 gactggatga ccggagcctt cgagaccgtc tttgcgcttc ccggtgactt gcagtttcgt
    10021 accagcggaa cactgttcgg gcatcgggtt cttcaggagc gactggccat caagagcggc
    10081 cagccgattc tgaccagcaa tttctacgag ttttaccgcg agtgcatcac gcccgagctc
    10141 gcctcgggct atatccgcat gaacgacctc atgaagacga acgacatctg gggcacgctc
    10201 aacggattga ccaatccctc ccggctggtg accctccggg acacggcgga tccgaccatc
    10261 atgcaaacgc tggggtgcac tgccgcctat acgacgctca ccacgcagtt gaacgccgag
    10321 gccgccaatc aactgggcct gctgggcaat cggatgtatc cgggaatgcc ctccgccctg
    10381 gccaatacgg cgatctcagg gtcgctcgcg acgagcacca attatcttct cgggctatcg
    10441 gcgacggcga ccgataccat caagcagtcc atcgtctcaa acgccgtgat tgacgcccag
    10501 tacaccatcc cggcacagat cggagacgcg gcaagtgctg cgaccaatct ggcacaggca
    10561 caggccgtgc gccagaccgg tgacgcctac aaggcgatgg caaagctggc cgagggcacc
    10621 atgcccaaag cccgaaacgc catcgagctg gttcaatacg ccgtcttccc gatcattctg
    10681 ctactcacgc tcgttgccgg tcaccatggc ggatcggtgc tcaagatgta cgccggcagc
    10741 ctgctctgga tccagttgtg gccaccgctc tacgcgatca tgaatttcct catgaatgta
    10801 caggcgcaaa aggcactgac cagcgcgacc aatggcgggg ggatgtctct ggagtcctac
    10861 agctatctca acaactccgt ggtcagcgat caggccattg ccggaatgct ggtgttgtcg
    10921 attccggcga ttgccgcggc gctcatcaag tggggcgatg ccgggctgag atcggtcggc
    10981 gcgtccttcg cgccacgtga tgcagaaaag gccgccgccg ccctggcgca gggcaacatg
    11041 aacatgggga atgcctcgct tggcaatgtt cagcacggga attactctgc cttgcagcat
    11101 tccacgcagc cctcactcgc ggcaggcgcg ggcaaaatgg catcggcgga cagctttggc
    11161 tcgatggagc gcacggggga tgccgtgacc gtaaaaagcg gcaacatgac gatgaccggg
    11221 ttcgacagga atcgtgatgg ccgatttacg atcgacgagg taacgaatgt ctccgcagca
    11281 ggatcgtcgc tgatgggcgc catcgcacaa tccgggttag gacgctcagc gtcggatgtc
    11341 caatactccg gtgagggtac gcaagcctct acgggcaaag ccagcacaat aggggattcg
    11401 aaaactgcga aactcaccca ggatcaaagt gccgtgtttc aacggcagtt atcgaaggca
    11461 ttgaattcgg agattggcgg agacaccagt gctggcgtga cttcaggtgc cggcaacgcg
    11521 gtttccacgg ggcgagactt ttcagccaaa aaaggtctga ccaatcacga aggcgcgtcc
    11581 atggattccg tcctgaccgc aggtggtagt ggtcaaagca agcctggaga gggacaggcc
    11641 tcaaaggatc atggaagagc tgggcagctg gcaatggcac ttgcggaaaa ggcctccgga
    11701 gtatcggccc gtgtggcagg aggcgtcaag accgggcagc gctatgacga gtctgccgat
    11761 caaacgctca agactgcaac acaggacgag ttaaggcagg ccgccgaggt agccaagcgt
    11821 ggtctgcttc gtgttgctgg atcaacctct gatcaaggcg taaaacaagc agcggaacgt
    11881 gcagccgcta ccctcgacaa agccaagtca ttcgacagta aggagctcgc ctcactgacg
    11941 gagcagcaag aggctggcag tcgtcgccag gcgggaaatg agaaccgctc caatgtttcc
    12001 gtggatgaat ccctgatcat ggcgaagtct ggctatgaaa tgctctttgg gaagggctct
    12061 gaagtcacac cggagcgcct ggctgcgttt cagcgcgagt ggaattcgaa tcagggcttt
    12121 cgcgaagatg ttgccttgga gaccagggac cgtcttgcca agtctcgtct ggctgcggaa
    12181 ggggttgagc cacccatatc ccaggacact gttcgagccg aaggccaaac ggcgctcgct
    12241 gcactggata gcaaaggaaa cgatgcggtt gcatcacagg ggcgggcaaa cagcaggcac
    12301 gtagaacgac agcaatatca gaatccacga tccatgccgg atctgtcacc agcagcgaag
    12361 acatacgccg aatcgtttaa cgctgcgtct gaacaagtca agcggaagga atcgcaggtg
    12421 tcgcttgaaa gcgggattac tgtcatggcg aatcagttgt atcaggagcg ccaacgaggg
    12481 acgggaaccg tgctcttgaa tacctacgga tctggtgccg ggtcagccag tgttcaggat
    12541 tacgcgggca agttgagaga ggcttcgcaa aaggatcccg aactagccca aggcttagcg
    12601 accataggca gcaataatcg ggccggcgtc caaccctcgc acgctgacat ggcgtggctg
    12661 gaaggaaagg ctaaacagtc tgtacgccag gcggaaggaa gtgcttctga gggagcgggt
    12721 tgggttgccg atcaagcagg aaaagcactg gatctgtttt cgccgagtag ctcaccttcc
    12781 ccaagaaaat gattgcaaag aggcaggagg gaatcagtcg tcgtcgaggg ggtcctgagc
    12841 gatacggacg ccgcctgcgt acatcccaaa gccctggaag ccggggccca cgccagagaa
    12901 gttcagtgag ttcggatcct gatcatgtgg tggatcggag aaatacgatt gctgcctggg
    12961 atcattctcg ccattacgca gtacaaagcg aataaatgcg actgccagaa aaacccagga
    13021 gagcagggcg gcgaccagga ttgatttttc gttgccgaac atcgctacca aagtggcggc
    13081 cacaagcgct gcaacgagga gcagaagcga ctcgacgaca tgccccgttc gaaacagctt
    13141 cacggcgaca gccatcaatc gggcagccag atagatcagg gtcatccctg gtacgaccag
    13201 cagcacagat acaagaacca ttctttccat tgggtactcc aatacgccat tcatctaact
    13261 tttactatac gcgcaatgcg cgtatagtaa aagcgttatc aatcctgatt gccacgatgg
    13321 ctgctactgg ttatcccaca cccactgaga ttcgagcggc ccgaatacta cttggagtat
    13381 ctcaagcgca agcggcttta ctggttcatt caacgtgccg agcatggcag caatgggagg
    13441 ctggggatag aaagatgcat ccggctttct gggagctttt ccgaatcaag gctgtactca
    13501 atgcgcgcat atcaaagcag tagtcctgct gatgctgaac aaggaattcc atggatttgc
    13561 agccggcatt catgttccat gcatcgatgg aggattgttc ggcggtacta tccttttttc
    13621 caaaacctga tcacggagat cccatgaata aatctgagct tgtcgaaatc gtcgccaagg
    13681 aagcggacct gtccaaggcg gctgccggga gtgcgctgga tgcagtcatt gctgccatcg
    13741 tgaaagctgt atccaagggc gataccgcca ccttgattgg attcggcaca ttcaagccct
    13801 cgaagcgtgc tgcccgcatc ggtcgcaatc ctcagaccgg caaggaactc aagattgcag
    13861 ctaccacggt gcctcgtttc acagcgggtg c
//
LOCUS       DAORPM010000027        13864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_887_length_13864_cov_10.788689, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000027 DAORPM010000000
VERSION     DAORPM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_13864_cov_10.788689"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLD53_02450"
     CDS             <1..417
                     /locus_tag="PLD53_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRC59796.1"
                     /translation="WRGGRGILENIIPSSTGAAKAVGVVIPELNKKLTGMAFRVPTSD
                     VSVVDLTVELNKEATYKEICAAMKAASEGAMKGVLGYTDEKVVATDFRGESCTSTFDA
                     DAGLALDGTFVKVVAWYDNEWGYSCKVLDMVRVMAK"
     gene            488..1657
                     /locus_tag="PLD53_02455"
     CDS             488..1657
                     /locus_tag="PLD53_02455"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRC59797.1"
                     /translation="MTFKRLTDLDVAGKRVFIRADLNVPQDDNLAITDDTRIRAALPG
                     IQYALSKGAAVMVTSHLGRPTEGEFKPDDSLAPVAKRMSELLGKEVRLVQNWVDGGFE
                     VKPGEVVMLENSRCNKGEKKSNDELSQKMAALCDVWVMDAFGTAHRAEATTYGIGKYA
                     KIACAGPCVASELEALTKALASPARPMVAIVGGSKVSTKLTVLESLSAKVDQLVVGGG
                     IANTFLLASGKNIGKSLAERDLVDQAKAVMAKVKVPLPTDVVCAKEFSATAPATIKSV
                     DDVADDDMILDIGPDSAKALADIIAKAGTVVWNGPVGVFEMDSFANGTKALAAAVASS
                     KAFTLAGGGDTVSAINVFKIEDKVGYISTAGGAFLEFLEGKRLPAVDILESRFKD"
     gene            complement(1890..2321)
                     /gene="tnpA"
                     /locus_tag="PLD53_02460"
                     /pseudo
     CDS             complement(1890..2321)
                     /gene="tnpA"
                     /locus_tag="PLD53_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043768368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            2736..4289
                     /locus_tag="PLD53_02465"
     CDS             2736..4289
                     /locus_tag="PLD53_02465"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRC59798.1"
                     /translation="MAIKPELIEILRTRRGKITEHVSPEKLDALHAKGLLSARERINW
                     LFDEGTFQEVGMHAKHHAVHFGMAGRELPADGVITGTGYLGSVQVASYSQDFNVLAGT
                     LGKMQARKITRIMRYALKTGVPVVAFKDSGGARIQEGVDALSGYGDVFYSNVMLSGVV
                     PQIAVVCGPCAGGAAYSPALMDFVIMTRKNSHMFLTGPEVIKAVTGRATTMEEVGSAT
                     MHSSVSGNVHFVAEDDQSAIALVKRILSFLPANNTEDPPHNLDTMIEDTPDRGLEELV
                     PDSPSEPLDMESIIRRLVDNGELLEVHADFARNVIVGFARFSGVIVGVVANQPKVMAG
                     ALDLNAADKVARFVRTCNVFNVPLVTLVDVPGFLPGVEQERGGIIRHGAKMLHAYASC
                     TTPKITVLLRKSYGGSYLAMCSQELGADMVFAWPTAEIAVMGSEAAVKILYRKELDAA
                     EDRAARAAELAQEYRDEFASPYEAAANFYITDVIEPCETRAAISIALRKTLNKRELRP
                     SKKHGNMPL"
     gene            4310..4627
                     /locus_tag="PLD53_02470"
     CDS             4310..4627
                     /locus_tag="PLD53_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59799.1"
                     /translation="MFSLAAIKAIQIYGIAIAISMLVAVLIKLLVVLTSRAEPVVKAV
                     ATVQKSADLGAVAGVPSEVVAAISAAVAVVTGPHHVLRIAESKRSWANEGRIAQHSHQ
                     PRH"
     gene            4646..5047
                     /locus_tag="PLD53_02475"
     CDS             4646..5047
                     /locus_tag="PLD53_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59800.1"
                     /translation="MPRKFKVTVNNVDYDVDVLEIGAGSPQAMPSYTPIAAQIAPISS
                     VPAAAMVAAAPSAKTGPAGAGDQCAQMGGVVVNIPVKQGHSVNEGDVVVELEAMKMKV
                     PVIADRSGQVARILVAVGDGVENGQALLTIA"
     gene            5168..6373
                     /locus_tag="PLD53_02480"
     CDS             5168..6373
                     /locus_tag="PLD53_02480"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRC59801.1"
                     /translation="METINFIDLFQGIATLAASEPKIMFGRIFLMLLGFFLMYLGSRN
                     VLEPLLMIPMGLGMSTINAGVMFLEGGKMGTLFVDPLMSDPTDLVNIMQINWLQPIYT
                     LTFSNGLIACLVFMGIGVLLDVGYVMARPFQSMIIALFAELGTIAVFPIAVGLGLTEQ
                     EAASVATIGGADGPMVLFTSLVLAKHLFVPITVVGYLYLGLTYGGYPYLIKWLIPKKL
                     RGINMAADVGPTITRQQKLVFAVVACTLLCLLFPVAAPLFFSLFLGVAVRESGLQNFA
                     DVIGETFLYGATFFLGLTLGVLCEANTLLDPTVLKLLLLGILALLISAIGGIVGGYVL
                     YFATGKKFNPMIGIAGVSCVPTTAKVVQKIATGVNPSAIILPQALGANISGVITTAII
                     TGVYIALLR"
     gene            6680..7462
                     /locus_tag="PLD53_02485"
     CDS             6680..7462
                     /locus_tag="PLD53_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59802.1"
                     /translation="MTVHLYFSLIPEALIASNLPPEKFGQYYSTGSGYKSKGQSLFFE
                     VAPDFRNDYFDIETAIERCVPAADGTPKHSVYISVYRVLEHLPISALGKLYLTTAYGH
                     TLGLDRGVLTPERDSVLHLYQDLSPTNSLVVSNLDPVTFYESVTTSPTKFIRFPALCF
                     VELELDELATDPENGQANNLPYSFMHHLREALMVLEPRSGDKPSTKDSKMVHRVHSLE
                     FPYRMIKKGFYIGNGADLVFYPMFNHRELRDNYNQWWRSANI"
     gene            7988..9427
                     /gene="pyk"
                     /locus_tag="PLD53_02490"
     CDS             7988..9427
                     /gene="pyk"
                     /locus_tag="PLD53_02490"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRC59803.1"
                     /translation="MSRHTKIVATLGPASRDPLVIERLIRSGVDVVRLNFSHGNKDDH
                     IALAASVRRIAAQVDRPVGILADLQGPKIRIGKFIVGKITLEAGDHFILDAQCEQGDQ
                     QRVGLDYKDLPKDVSSGNVLLLDDGRIVLDVERVAGKEIHTTVRHGGELSNNKGINRQ
                     GGGLSAPALTAKDMDDIKTAALIGADFLAVSFPKGAPDMYMARQLMLACGGHALTIAK
                     IERVEAVAALEEILDAADGLMVARGDLAVEVGDAAVPALQKRMIRLARTKNKLAITAT
                     QMMESMILSPTPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPVQTVESMARICLEAE
                     KSILITLDRDFLDRIFTRIDQSIAMAAVFAAYHLNVKAVAALTTSGSTALWMSRLNCG
                     IPIFALTPEPTARSKMTLYREVFPLLMKETHQDPDILRYEAEQRLIEAGVVTKGDLIV
                     LSYGDQMGLTGGTNTMTIVRIGDRAAPKT"
     gene            9683..11011
                     /locus_tag="PLD53_02495"
     CDS             9683..11011
                     /locus_tag="PLD53_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HRC59804.1"
                     /translation="MHARFNLTDIEQHPLREYLNNEFHARTPVPLNNPSLISHLAFQH
                     DGLSGQDERENVLRICQTSPCKFIENSATHLLLDAGNFQMRWELHTEYSSYTFFRPLQ
                     AGEPRHPDATAFDSVFPDWLSAIPGKLIVASHIELRSTEDVAPESVFSSLSPKSGRQM
                     VVSRIANGAAWVFTDFMFDNGFSRFLLLDESLTGRQPGRAVQRLFEIETYRMMALLGL
                     PVATEVGNWLNGAESRLAELMDHIVSASTPEDERSVLTDLSKMAAEVEHSVARTTVRF
                     GASHAFNSLVWQRIEELREIRVPGYQTLHEFMQRRFHPAMSTCAAMAKRQEDLSCRVA
                     RNSQLLRTRVDIELKRQNQEQLTQMNTRAKVQLRLQETVEGLSIVAITYYGSQLVQYL
                     TKGGKYLFPVISPEVATAISIPLIAILALFGIKRMRKTLALEAEDTNETD"
     gene            11123..11899
                     /locus_tag="PLD53_02500"
     CDS             11123..11899
                     /locus_tag="PLD53_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRC59805.1"
                     /translation="MSDPESPPGQGFALPSVRRVSLARPRNWLKSGWHDMRSNPVTSL
                     SYGLLFGIAGAVVLIFSWSKPYLFTAAVSGFILIAPLLAGGLYEISRRQAEGQPATLL
                     ESLDGWRRNGQSMAMFGLLLALVALVWERTSAVIFALLVPGMAPDLSSFIFDVLLNPE
                     FAGITLAWFMVGTCLAILVFAVSAVSIPMLIDRDVDFVTAMITSLRATVLNPDVMFLW
                     AVLVVTLTLAGFATLLLGLVLTMPLLGHASWHAYQDLVEK"
     gene            11968..>13864
                     /locus_tag="PLD53_02505"
     CDS             11968..>13864
                     /locus_tag="PLD53_02505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRC59806.1"
                     /translation="MDSATTNPLLDFSGLPRFDAVTPADVQPAIGELLDQARTVIGQL
                     TTDEVKASWDGFVIPLDDCIEQLTRAWGIVCHLHAVNDIPEWREAYNRMLPDVSRFNA
                     ELGQNLRLFDKYRALRDSSEYANLSTAQRRIIENEIRDFRLAGAELPEDKKSRFKAIS
                     EELSSLSAKFSENLLDATNAFAEIVTDEAGLTGLPDDVIEAAREAAEKDGKAARDGKY
                     SPAQTWKFTLHMPSYLPVMQYADSRRLRAVMYRANATRAAEFGPTEFDNTPLIRRILE
                     LRREEAQLLGYNNFAEVSLVRKMAESVPQVLSFLRDLAVKARPFAQKDIAELREFARR
                     ELALETLEAWDVSYASEKLLQARYAFSEQEVKQYFTEPKVLNGLFQVVENLFGVCIKP
                     DEAPVWHEDVRFFRIEVRDGEHAGELIGQFYLDLYARETKRGGAWMDEAITRRRISTG
                     ETMGDIQKPVAYLNCNFSRPVSGRGGQLRPATFTHDEVTTLFHETGHGLHHLLTQVEE
                     LGVSGIHGVEWDAVELPSQFMENYCWEWSVLQQMTAHIATGAPLPRALFDKMLAARNF
                     QSGLQIIRQIEFSLFDMRLHSDFDPSAGDAGNSSVLEVLAEVRREVAVIVPPEWNRFP
                     NSFSHIFA"
BASE COUNT         2972 a         4080 c         3730 g         3082 t
ORIGIN      
        1 tggcgcggcg gccgtggcat cctcgagaac atcatcccgt cctcgactgg tgctgccaag
       61 gctgtcggtg ttgttatccc cgagctgaac aagaaactca cgggtatggc attccgcgtt
      121 ccgacttccg acgtatcggt ggtcgatctg accgtcgagc tgaacaagga agccacctac
      181 aaggaaatct gcgccgccat gaaggcagct tccgaagggg cgatgaaggg cgtgctcggc
      241 tataccgatg aaaaagtcgt cgccaccgac ttccgcggcg aaagctgcac gtcgaccttc
      301 gatgccgatg ccggcctcgc gcttgatggc acctttgtca aggttgtggc ctggtatgac
      361 aacgaatggg gctactcctg caaggtcctc gacatggtcc gtgtaatggc caagtaatac
      421 ccgtgctcag gggcacttcc gggtgcccct ttcttttttt aatccaagtt ttgaatcgag
      481 aatcgccatg accttcaaac gtctcaccga ccttgacgtc gccggcaagc gagttttcat
      541 tcgcgccgac ctgaacgtac cccaagacga caacctcgcc attacagacg acacccgtat
      601 ccgcgctgcc ctccctggca tccagtacgc gctttccaaa ggtgccgccg taatggttac
      661 ttcccacctt ggccgcccga ccgagggcga gttcaaacct gatgactctc tggcgccagt
      721 cgcaaaacgc atgtcggaac tgctcggtaa agaggttcgc ctcgtccaga actgggtcga
      781 tggtggcttt gaagtcaagc cgggcgaagt cgtcatgctg gaaaacagcc gctgcaacaa
      841 aggcgaaaag aagagcaacg atgaactttc gcagaaaatg gcagcgctat gcgatgtctg
      901 ggtaatggat gccttcggca cagcgcaccg cgctgaagct accacctatg gcataggcaa
      961 gtacgctaaa atcgcttgcg ccggaccctg tgtcgcctcc gaactcgaag cactgaccaa
     1021 agcccttgcc agccccgctc gtccgatggt ggccatcgtc ggcggctcca aggtttcgac
     1081 caaactgacc gtactcgaaa gcctttcggc caaggttgac caactggtgg tgggcggcgg
     1141 tattgccaac accttcctgc ttgcctccgg caagaacatc ggcaaatcgc ttgccgagcg
     1201 cgatctcgtc gatcaggcga aggctgtcat ggccaaggtc aaggtgccct tgccgaccga
     1261 cgtcgtctgc gccaaggagt tctccgcaac ggccccagcg acgatcaaga gcgttgatga
     1321 cgtcgccgac gacgacatga tccttgacat cggcccggac tcggccaagg cattggccga
     1381 catcatcgcc aaagcgggca cggtcgtctg gaacggcccg gtgggagttt ttgaaatgga
     1441 ttcgttcgcc aacggaacca aggcgctggc agccgctgtc gccagctcga aggcattcac
     1501 gctggccggc ggtggcgata cggtttcggc aatcaacgtc ttcaagattg aagacaaggt
     1561 cggatacatc tcaaccgcag gcggcgcctt cctcgagttc cttgagggca agcgtctgcc
     1621 ggctgtcgac atccttgagt cacgcttcaa ggactgaacg gcgaacccgt ctggcacaga
     1681 cgaaggctgc cattgagcag ccttcgctgt tttcaccagt cgcaaagtta atcatcctct
     1741 atgtacatga catgtgtgcc gggtggtttt gaagtaaccg ggtaaatatc ccccagcaga
     1801 gccggcggca ttaatttgtg agccgctcta agcggcaggt cggggtcgct aggccccaca
     1861 gctcaggtgc cagcgtatcc ggcggcagct catctccaaa ggccgagctg atccagtcgc
     1921 tcgtcctctt tctcctggtt cttgatgtac tcccgtatca ctgcctcgtc gcggccgact
     1981 gtttatacga ggtatccgcg agcccagaag tgttgcccga cgaaattgcg cttgcgctct
     2041 ccatataccc tggacaggtg aatgacgctc ctgtccttga tgaaaccaat cacccgcaat
     2101 accgcatact tcggcgggat cgcgatcatc gtgcgcacgt gatcggacat caggtgtcct
     2161 tccacgatct ggctttcctt ctgcacagca agcttccggt actcttctcc cagatgctgc
     2221 cgtaacccca cctataacgt cctgcgtcga cgcttcgcgg tgagtattgc gtgatatttg
     2281 caatcccacg ccgtgcgact ccaactttcc aacttgtcca tcgtatttct tccttttgtt
     2341 tgcttggcgg ctcacgattg gaagcttcta cgatggacaa gcccgagaat gccaaactcc
     2401 tagtgcctcc ccggcagagc cgggggagcc tccctttgtt actcgcagca caggcgcctg
     2461 attcgagtcg atgctattac gcacggtgca ggtgctggac gccaatccaa aaaaaccatc
     2521 gtctcgcaag ccgccgttaa gcaattggca agcattcttt aagttattgt atttatttaa
     2581 taaattgtac atttgtaagc gcggcgtaag cttgaattcc taccgtggcc tccgcagtca
     2641 tcccgaatga ctcgactaac cgtccctgtg cgaggaggcc cgcaagtctg acggtcgggc
     2701 aacgacctat taaagatcac ttggcaaagg cacccatggc aatcaaaccc gaactgatag
     2761 aaatcctgcg aacaaggcgg ggaaagataa ctgagcacgt tagccccgag aaacttgatg
     2821 ccttgcatgc caagggtttg ctttcggctc gcgagcggat caactggctt ttcgacgaag
     2881 gaacctttca ggaagtcggc atgcacgcaa agcaccacgc cgtgcatttc ggaatggcgg
     2941 gacgcgagtt gcccgccgac ggcgtgatca ctggcacggg ttatctggga agcgtacagg
     3001 tcgccagcta cagccaggat ttcaatgtgc tggccggtac gcttggcaag atgcaggcgc
     3061 gcaagatcac ccgcatcatg cgctacgctc taaagaccgg cgttccggtt gtcgccttca
     3121 aggactccgg cggagcgcga attcaggaag gtgtcgatgc tctctccggt tacggcgacg
     3181 tcttttactc gaacgtaatg ctctcgggcg ttgtgcccca aatcgcagtc gtgtgcgggc
     3241 cctgcgcggg gggtgcggcc tactcgccgg ccttgatgga tttcgtgatc atgacgcgca
     3301 agaattcgca catgttcctg acgggacctg aggtgatcaa agcagtcaca ggtcgcgcaa
     3361 caaccatgga ggaagttggc agcgccacga tgcactcgtc cgtcagtgga aacgttcact
     3421 ttgtcgctga agacgatcag agtgcgattg cgttggttaa acgcattctt tcgttccttc
     3481 ctgccaacaa taccgaggat cccccacaca atctcgatac catgatcgag gacaccccgg
     3541 accgtgggct ggaggaactc gttccggact ccccgtcaga accgctagac atggagtcga
     3601 tcataaggcg cctagtggat aacggtgaac tgctcgaggt acacgccgat tttgcccgca
     3661 atgtcatcgt gggcttcgcc aggttttcgg gcgtgattgt cggagtcgtt gccaaccagc
     3721 cgaaggtaat ggctggagcc cttgacctca acgccgccga caaagtggcg cgcttcgttc
     3781 gtacctgcaa cgtattcaac gtgccgttgg ttaccctggt tgatgtccct ggattcctgc
     3841 cgggggtaga acaagaacgt ggcggaatca tccggcatgg cgccaagatg cttcatgcct
     3901 atgcttcctg cacaacgcca aagatcactg tattgctacg caaatcctat ggtggctcct
     3961 atcttgcgat gtgcagtcag gaattggggg ccgatatggt gtttgcctgg ccaaccgctg
     4021 aaatcgccgt catgggttcc gaagctgcgg tcaaaatcct gtaccgcaag gaacttgacg
     4081 cggccgagga ccgcgctgcc cgcgcggccg aactggcaca ggagtaccgc gatgaattcg
     4141 cgtctccgta cgaggcggct gccaattttt acattaccga tgtcatcgag ccctgcgaga
     4201 cccgggccgc catttcgatc gcgctacgca agacgctgaa caagcgcgaa cttcgcccca
     4261 gcaagaagca cggcaacatg ccgctgtaaa gcccggagga ccaacatcca tgtttagcct
     4321 ggccgcaatc aaagcgattc agatttacgg gattgccatc gccatctcga tgctcgttgc
     4381 cgtactgatc aagttgctgg ttgttctgac aagccgtgcc gagcccgttg tcaaggcggt
     4441 cgcaaccgtg cagaaatccg cagacttagg cgcagtagcc ggagttccgt ctgaggtggt
     4501 cgcggcaatt tcggcggcag ttgccgtcgt caccggaccg catcacgtat tgcgtattgc
     4561 cgaaagcaaa cgttcttggg caaacgaagg tcgtatcgcc cagcattctc atcaaccaag
     4621 acactaaatt gcaaggcgaa atatcatgcc aagaaaattc aaagtcaccg tcaacaacgt
     4681 cgattacgac gttgatgttc tcgaaatcgg tgcgggatcc ccccaggcca tgccttctta
     4741 cacaccgatt gcggcacaga tagctccaat cagctcggtt ccggccgccg cgatggtcgc
     4801 cgctgcacct tcggcgaaga ctgggccagc gggcgccgga gatcaatgcg cccagatggg
     4861 tggtgttgtt gttaacatcc cggtaaaaca gggacatagc gtcaatgaag gcgacgtcgt
     4921 cgtcgaactt gaagccatga aaatgaaggt tccggtgatc gcagatcgct ctggtcaagt
     4981 cgcgcgcata cttgtcgcgg taggcgacgg cgtcgaaaac ggtcaggcat tgctgaccat
     5041 cgcctgatcg cgtctacgta ggcagtctgt cggccccgct ctcaaacccg gtaccccctg
     5101 tgccagccgt tttcgcatcg caatagccac tggcggttcg acccgcctgc taagaggtct
     5161 gaatcccatg gaaaccatta actttatcga tctctttcag ggtatcgcca ccttggcggc
     5221 ttccgaaccg aagatcatgt ttggtcgaat ctttctgatg ctgctcggct tcttcctgat
     5281 gtatctcggt tctcgaaacg tacttgaacc tttgttgatg atcccgatgg ggctgggcat
     5341 gtcgacgatt aatgctggag tgatgtttct cgaaggcgga aagatgggaa cgctgttcgt
     5401 cgacccgctg atgtccgacc cgacggatct ggtcaacatc atgcaaatca actggctgca
     5461 gccgatctac acgctgacct tcagcaacgg cctgatcgcg tgccttgtgt ttatgggtat
     5521 cggcgtacta ctggatgtcg gttacgtcat ggcgcgccct ttccagagca tgatcattgc
     5581 cttgtttgct gaattgggaa ccattgccgt tttcccgatt gccgtcggct tgggccttac
     5641 tgagcaggaa gccgcttctg tcgccacgat cggcggtgca gacggcccaa tggtgttgtt
     5701 cacttcgctg gttttggcca agcacctgtt cgttccgatc accgtggtgg gctatttgta
     5761 cctcggcctc acctacggcg ggtatcccta tctgattaag tggcttatcc cgaaaaagct
     5821 gcgcggcatc aacatggctg ccgatgtcgg cccaacgatc acccggcaac agaagcttgt
     5881 ctttgccgta gtcgcctgca cgctgttgtg cctgttgttt ccggttgcag cgccgctgtt
     5941 cttttccctt tttctcggcg tcgccgtgcg cgaaagtgga ttgcaaaact tcgccgatgt
     6001 catcggcgag accttcctct acggcgccac attctttctc ggactcacgt tgggcgtgct
     6061 ctgcgaggcc aacaccctgc tcgatccaac ggtactcaaa ttgctgctgc tcggcatcct
     6121 ggcgcttctg atttcagcga tcggcggaat agtcggaggg tatgtccttt atttcgcgac
     6181 cggaaagaaa ttcaacccga tgatcggtat tgccggggtc agctgtgtgc ctacgaccgc
     6241 caaagtggtg cagaaaatcg cgactggcgt caacccatcg gcaatcatcc tgccacaagc
     6301 cctaggcgcc aacatcagcg gggttattac cacggcgatc attaccggcg tatatatcgc
     6361 gctgctgcgt tgaccacgcg ttctcttgcg cgattcttcc tgatcgtgcg agaaatcgct
     6421 tcacggtgat cagtgcccag atcgccatcg actcgaattc ttcgcgcgtc gctcgaactt
     6481 gaccgaactg ccctatccgc catcctgccg atcgcacctt ccggcaagac catatttcgc
     6541 ttgtttaccg aatgacattg acgcggcgcc gccatataat taccccagtg gttaactagc
     6601 acaatcttga tagcgcaata ttcgtttagt ccgattcatt tagttgtgat ccattcaata
     6661 gtgggaaggg tatgcagcca tgactgttca tctttatttt tctctcattc ccgaggcgct
     6721 gattgcctcc aatcttccgc cggagaaatt cgggcagtat tactcgacag gtagcggcta
     6781 caagtcaaag gggcaaagcc tgttctttga agtcgcccca gacttccgga atgattattt
     6841 cgatatcgag acagccattg aacgctgcgt tcccgccgcc gacggcacgc caaagcactc
     6901 tgtttacatc tctgtctatc gggtactgga acatctcccg atcagtgcgc taggcaaact
     6961 ttatctgacc accgcctacg gccacacctt ggggcttgat cgtggcgtat tgactccgga
     7021 aagagactcg gttctgcatc tttaccagga tctttcgcca accaacagtc tggttgtcag
     7081 caatctggac ccagtcacat tctatgagag cgtcaccacc agcccgacca agtttatccg
     7141 tttccccgca ctctgctttg tcgaactcga actcgacgag ttggcgactg acccggaaaa
     7201 cggccaagcg aacaatctcc cctactcgtt catgcatcac ctgcgcgagg ccctgatggt
     7261 tttggaacca agatccgggg acaagccgag taccaaggac agcaagatgg tacatcgcgt
     7321 acactcgctg gaattcccgt accgcatgat caaaaagggt ttctatatcg ggaatggcgc
     7381 cgatttggtg ttttacccga tgttcaacca tcgtgagttg cgggacaact acaatcagtg
     7441 gtggcgttcc gccaacattt gatagctcta cagtcgtatt tcagactgtc agctacagcg
     7501 cgagccatca acaatcaagc gaccctgtcg ttgacggcgg ttgaatgaaa ttcccggagc
     7561 tttcacatgt ccgggtattc gtgctacctc cgttggcggt ggtgcttggt tcacccgccc
     7621 cttcttctac tcctcgtgta gcaatcccgg ttttagggct gaaggactgt tggaagcgtc
     7681 attcccggtc gccaggatgc tcatttcagg atagtgccgg ggacgcgctc atcagccctg
     7741 cagcacactc agagcagcgc gtaatcttgc tgcaacaccc ctgctctaaa ctcatgccct
     7801 gctagtttgg cgaggtcgtc tcgccaaact agcatttccc cgataggtga ggggtgatgc
     7861 aaccccgcga caggttttcg gcgcaatgat gaaacagccg ctagaatgcg ggtttacgca
     7921 cagcccgcaa gcgcgaattg gacgaaagaa gagcggtttg ctcgggcctg ataacctaag
     7981 gaagtgaatg tcccgccata ccaagatcgt agccaccctt ggccccgcat ctcgcgaccc
     8041 actcgtcatt gaaagactga ttcgttccgg cgtagacgtg gtgcggctca acttctcgca
     8101 tggcaacaag gacgatcaca tcgccctcgc cgccagcgtg cggagaattg ccgctcaagt
     8161 ggaccgcccg gtcggaatcc tcgcggactt gcagggtcca aaaatccgca tcggaaaatt
     8221 catcgtcggc aagatcactc tggaagccgg cgatcatttc attcttgatg cccaatgcga
     8281 acaaggtgat cagcaacgag taggtcttga ttacaaggac ctgcccaagg acgtcagttc
     8341 cggcaatgtg ctgttgcttg acgacgggcg cattgtgctt gacgttgagc gtgtcgccgg
     8401 caaggagatt cacaccaccg tacgccacgg cggcgaactc tcgaacaaca agggcatcaa
     8461 ccgccagggg ggtggcttgt cggccccggc acttactgcc aaggacatgg acgacatcaa
     8521 gacagccgcg cttatcggtg ccgactttct cgccgtttcc ttccccaagg gcgctcccga
     8581 catgtacatg gcgcgtcaat tgatgctggc ttgcggcggc catgcactca ccattgccaa
     8641 aatcgagcgc gtcgaggcgg ttgcagcgct ggaagaaata ctcgacgctg ccgacggcct
     8701 catggtagca cgcggcgatc tcgctgttga agtcggtgat gccgccgttc ccgcattgca
     8761 gaaacgaatg attcgtttgg cgcgcactaa aaacaagttg gcgattacgg cgacacagat
     8821 gatggaatcg atgatccttt cgccgactcc gacccgtgcc gaagtttcag acgtggcgaa
     8881 cgctgtgctc gacggcacgg acgcagtcat gctttcagcc gaaacggcgg caggcaaata
     8941 cccggtgcaa acggtcgagt ccatggctcg catctgcctg gaagcagaaa aatcgattct
     9001 gattacgctc gatcgcgact ttctcgaccg gatttttacg cgaatcgacc agtcgatcgc
     9061 catggctgcg gtttttgctg cctatcacct caatgtcaaa gccgttgccg cactaacgac
     9121 ttccggctct acagcgctct ggatgagtcg gctgaattgt ggaataccga tcttcgcatt
     9181 gacgccggaa cctacggcgc gatcgaaaat gacgctatat cgtgaggtat tcccgctgct
     9241 gatgaaagaa acgcatcaag atcccgatat cctgcgctac gaagccgaac aacggttgat
     9301 cgaggctggc gtggtcacca agggagattt gatcgtgctt tcctacggcg accagatggg
     9361 tctgaccgga ggaacgaata cgatgaccat agtacgcatc ggtgatcgcg cggctcccaa
     9421 gacctgaacc ggcacacgag ttgccaggta ccgtcgtcat ccgttgctac gtccggcggg
     9481 ttgaattttg tgcatcactg gtagtcgaat catccagcag cacggtaagc ccaccaactt
     9541 tcagctcgca cagacatccc gacacaagca cattatcctg attctggaat tgtctcggca
     9601 agttccggca actcgtctgt gtcgccacgc gatttcttac atgaatcagg aagatcgaaa
     9661 taatcagaat tctggagaat gaatgcacgc ccgattcaac cttactgaca ttgagcaaca
     9721 cccgttacgc gagtatctga acaacgagtt ccatgcgcgt acgcctgtcc cgttgaacaa
     9781 tccctcgctg atttcgcatc ttgcgttcca acatgatggc ctgtccggtc aggatgagcg
     9841 cgaaaacgtt ctgcgaatct gccaaaccag cccgtgcaaa ttcatcgaaa attcggcgac
     9901 gcatctgctg ctggacgccg gaaactttca gatgcgctgg gaattacata ccgaatattc
     9961 gagctacaca ttctttcgtc cgctgcaagc cggcgagccg cgtcacccgg acgcgaccgc
    10021 ctttgacagc gtctttccgg actggctatc ggccattccg ggcaagttga tcgtggcttc
    10081 ccatatcgaa ttgcgctcaa ccgaggacgt tgcacccgag tcggtattct ctagcctttc
    10141 accaaagtct ggccgacaga tggtcgtctc ccgaattgcc aatggagcag cctgggtatt
    10201 taccgatttc atgtttgaca acggtttctc gcgcttcctg ctacttgatg aaagcttgac
    10261 ggggcgccag cccggccgcg cagttcagcg actgttcgag attgagacct atcgcatgat
    10321 ggctctgctt ggtctgccgg ttgcgacgga agtcggcaac tggctaaacg gcgccgaaag
    10381 ccgtttggct gaattaatgg atcacatcgt aagcgccagt acaccggaag acgaacgctc
    10441 cgttctgacc gacctctcga agatggcggc ggaagtcgaa cattcggtgg cacgcaccac
    10501 cgtccgcttt ggcgcctccc atgcgttcaa tagtctggta tggcaacgca tcgaggaatt
    10561 gcgcgagatt cgcgttccgg gctatcagac gctgcacgaa ttcatgcagc gccgattcca
    10621 tccagcgatg agcacctgtg ccgcaatggc caaacgccag gaagaccttt cgtgccgagt
    10681 ggctcgcaac agccagttgc tgcgcacgcg ggtcgatatc gagctcaaac ggcagaacca
    10741 ggaacagctg acccagatga acacccgcgc aaaagttcag ttacgcctgc aggaaaccgt
    10801 cgaaggtctg tcgattgtcg ccatcaccta ttacggctcg cagcttgtgc aatacctcac
    10861 caagggagga aaataccttt tccctgtcat ctcgccggaa gttgccaccg ccatttcgat
    10921 tccgctgatc gcgatcctcg ccttgttcgg catcaagcga atgcgcaaga cattggcact
    10981 ggaagcagaa gacaccaacg aaaccgattg atgcagaatc agtcccaccc gccatattca
    11041 tagcagcctg aatattgtcg accaagagcc ttggcgcccg gccggagtcg gagagcatga
    11101 tttacacctt ggaggagagc gcatgagtga cccagaatcc ccacctggcc aaggatttgc
    11161 cttgccttcc gtacgccgtg tcagcctggc tcgcccgagg aactggctca aatcgggttg
    11221 gcacgatatg cgctcgaatc cggtgaccag cctcagttat ggcctgctgt tcggcatcgc
    11281 cggcgccgtt gtattgatct tctcctggag caagccgtat ctgtttactg cggctgtttc
    11341 cggatttatc ctgattgccc cgttgctcgc tggcggccta tatgaaatca gccgccgaca
    11401 ggctgaaggt caacccgcga ccctgctgga atcccttgac ggctggcgcc gaaacggtca
    11461 atcgatggcc atgttcggcc tcctgctggc cctggtagcc ctcgtgtggg agcgcacctc
    11521 ggcggtgata ttcgcacttc tcgtccctgg aatggcgccg gatctttcgt cattcatttt
    11581 cgacgtgctg ctgaatcctg aattcgccgg aattaccctg gcatggttca tggtcggaac
    11641 ttgtctggcg atcctcgtat tcgccgtcag cgccgtatcg atacccatgc tgatcgatcg
    11701 tgatgtcgac ttcgttaccg ccatgatcac gagcctgcgc gccaccgttc tcaatccaga
    11761 cgtaatgttt ctctgggctg tgctggtcgt gacactaacg ctggccggct tcgccacact
    11821 gctcctcgga ctggtgttaa cgatgcctct acttggccat gcctcctggc acgcctacca
    11881 ggatcttgtc gagaagtgac aagtgactgt tgcaacaatt gatggcggct agggccgccc
    11941 aaacctgttt tggaaaaccg atatcccatg gactcagcaa cgaccaatcc cctgctcgat
    12001 ttttctggct tgccacgctt cgatgccgtc actcctgccg atgtccaacc ggccatcggt
    12061 gagctacttg atcaggcccg caccgttatc ggacagctca caaccgacga ggtgaaggca
    12121 agctgggacg gctttgtcat tccgctcgat gattgcattg agcagctcac gcgcgcctgg
    12181 ggcatcgtct gtcaccttca tgccgtcaac gacatacccg aatggcgcga agcctacaat
    12241 cgcatgctgc cggacgtctc ccgcttcaat gccgaactcg gtcagaacct tcggcttttt
    12301 gacaagtacc gggcactgcg tgatagcagc gagtacgcca acctctccac ggctcagcgc
    12361 aggatcatcg aaaacgagat ccgcgatttc cgccttgccg gcgccgaatt gcccgaggac
    12421 aaaaagtcgc gattcaaggc catttccgaa gaattgtcca gcctctcagc caagttttcc
    12481 gaaaacctgc tcgatgcgac caatgcgttt gccgaaatcg tgaccgatga agccggcctg
    12541 accgggcttc cggacgatgt catagaagcg gcccgggaag ccgcagaaaa agacggcaag
    12601 gctgcacggg atggcaaata ctcgccggct caaacctgga aattcacgtt gcacatgcct
    12661 tcctacctgc cggtgatgca atacgccgac agccgaagac tgcgcgccgt aatgtatcgc
    12721 gccaatgcta cccgtgccgc cgagtttggc ccgaccgaat tcgacaacac gccgctcatc
    12781 cgtcgcattc ttgaacttcg ccgggaagaa gcgcaactac ttggctacaa caacttcgcc
    12841 gaagtctcgc tcgttcgaaa aatggctgag tcggtaccgc aagttctttc gttcctgcgc
    12901 gacctggcag tcaaggccag acctttcgcg caaaaagaca ttgccgaatt gcgtgaattc
    12961 gcccgtcgcg aactggcgct tgaaacgctc gaggcctggg atgtcagtta cgcttccgaa
    13021 aaactattgc aggcgcgcta cgctttctcc gaacaggaag tgaagcaata ctttaccgag
    13081 cccaaggtgc tgaacggcct cttccaggtt gtcgaaaacc tgtttggcgt ctgcatcaag
    13141 cctgacgagg ctcccgtatg gcatgaggat gtccgcttct ttcgcatcga ggttcgggac
    13201 ggagaacatg ccggcgaact catcggccag ttctaccttg atctgtatgc ccgcgaaaca
    13261 aagcgtggcg gtgcctggat ggacgaagcg atcacccgac gtcggatctc caccggtgaa
    13321 actatgggcg acatccagaa accggttgcg tacctcaact gcaatttctc gcgccctgtt
    13381 tccggaagag gtggacaact gcggcccgcg acattcacgc acgacgaagt caccacgttg
    13441 tttcacgaga cgggccatgg gctgcatcac ctgctgacgc aggtcgaaga actcggtgtc
    13501 tcgggaatcc acggcgtcga gtgggacgct gtcgaactgc cctcgcaatt catggagaac
    13561 tactgctggg aatggagcgt gctacagcaa atgacggcgc acattgccac cggtgcccct
    13621 ttgccgcgcg cgctgttcga caagatgctg gcggccagga acttccagag cggcctgcaa
    13681 atcatccgcc agatcgaatt ctcgctattc gacatgcgtt tgcacagtga ctttgatcca
    13741 tcggcggggg atgccggcaa ttccagcgtt ctggaagttc ttgccgaagt acggcgcgag
    13801 gttgccgtta ttgtcccgcc agaatggaac cgttttccga acagcttctc gcatatcttc
    13861 gctg
//
LOCUS       DAORPM010000028        13850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_889_length_13850_cov_11.230083, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000028 DAORPM010000000
VERSION     DAORPM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 13850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13850
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_889_length_13850_cov_11.230083"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..685
                     /locus_tag="PLD53_02510"
     CDS             47..685
                     /locus_tag="PLD53_02510"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRC59807.1"
                     /translation="MTTVFISYSTKDHYFAELAGIKLAEAGINLWRDQGQLRPGSDWR
                     NGIERGISESIAVLVALSENSAQSSYVTFEWAYGLGKGKTVIPVKLEACAVHPRLEPI
                     QSLDFSIPGSLPWNLLIERIREIEADATSEEDLETSANTPEPPQQDATVKAILAYLNQ
                     RGYQMVSYERLRRRIDPALTDQKLDEVVNANPTVFRFARLKEGKRGMAKLIP"
     gene            746..1723
                     /locus_tag="PLD53_02515"
     CDS             746..1723
                     /locus_tag="PLD53_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRC59808.1"
                     /translation="MLTIFTTLADWIAFTLFGLAPHSKLGDAVHFFIEDTTKIFFLLV
                     VMIYFIALIRASLNVERIRDYLAKKNRLVGYFMGSIFGAVTPFCSCSSIPVFLGFTSG
                     GIPVGITMSFLLTSPLINEVAVLLLLSLVGWKITIVYVLVGLAVGITGGIFLDLIHAE
                     RLLQPFALKAYQSAKPAVGEGTSRSALSFGDRHEFAKQELLDILSRVWKWIFIGVGLG
                     AALHGFVPVAWIEANLGRGQWWSVPLAVFLGIPLYANATGIIPVMESLIAKGLPIGTT
                     LAFCMSTVAASFPEFILLKQVMTWRLLATIFVLLLASFTIVGWTLNAIY"
     gene            1739..1972
                     /locus_tag="PLD53_02520"
     CDS             1739..1972
                     /locus_tag="PLD53_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRC59809.1"
                     /translation="MKEIKVLGSGCAKCTKTAELIQTVATEFDASVNVVKETNPEVMM
                     NYGVMSTPAVVVDNVVVHSGSIPGRKMVEGWLK"
     gene            2107..2550
                     /locus_tag="PLD53_02525"
     CDS             2107..2550
                     /locus_tag="PLD53_02525"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRC59810.1"
                     /translation="MQTLPHHYRVAANAAPEGDVSLSSEKLETLPSAPPQEFGGPGDR
                     WSPETLLVAAIADCFVLTFRAIAKVSKLSWLSLRCDVEGTLERSEGKTKFTQFKANAT
                     LDVPPDTNEQRAQRILGKAETSCLITNSLSATTHLNAVVVAKSLA"
     gene            2695..3096
                     /locus_tag="PLD53_02530"
     CDS             2695..3096
                     /locus_tag="PLD53_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HRC59811.1"
                     /translation="MGFFSNILAKLGIGSDSAPAPTAAPATPTAPATPAATSAAPATP
                     AVALVDVVAQLEQRASANPQKLNWRTSIVDLLKLLDIDSSFAARKELATELGCPADLM
                     GDSAKMNIWLHKTVLARIAANGGNVPKDLLD"
     gene            3136..3756
                     /locus_tag="PLD53_02535"
     CDS             3136..3756
                     /locus_tag="PLD53_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRC59812.1"
                     /translation="MQITDILAQMGGLKSMAQELGVDEAQAASSAAALAPAILGGFKK
                     QAQSQPTGLEGFGGLLGKLGGGGLLDNVLAPQPTDVSQGNNVLGQIFGSKDVSRAVAQ
                     NVAEQSGLDPSLLKKMLPMLAMLVTGYLAKQQGTGAEAQPSPSAALGGGLGGGLGDLL
                     GGLLGGQNFGHAPQGAASSGLASMLDMNGDGNALDDILRIAGKAMR"
     gene            3809..4060
                     /locus_tag="PLD53_02540"
     CDS             3809..4060
                     /locus_tag="PLD53_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRC59813.1"
                     /translation="MNVIVSLIIGGIVGWLASIVMKTNAQMGLIANVLVGVVGSSLGY
                     WIAGMLGIAPTGILRFGVAVGGAALLIFILGKLGIFRRK"
     gene            4241..4774
                     /locus_tag="PLD53_02545"
     CDS             4241..4774
                     /locus_tag="PLD53_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRC59814.1"
                     /translation="MKKLSIALVVFMMGCTSIPEDVTPVDNFKLEKYLGKWYEIARLD
                     HSFERGLSQVTAEYSLRDDGGVRVLNRGYSAKDDSRKEAEGKAYFVKKPDQGYLKVSF
                     FGPFYGSYIVFELDENYQYSLVSGPDKSYLWILARRPEIGEDLKNTLTAKAAAAGFDT
                     SKLIFVDHDKSLASQPH"
     gene            4883..5290
                     /locus_tag="PLD53_02550"
     CDS             4883..5290
                     /locus_tag="PLD53_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC59815.1"
                     /translation="MIKVRDQKRAEAFYNGLLGMPIAARLPSFSMTFFTLGNHHDFAI
                     KAVGDSAPDAPEDSPGLFHVAFKIGSHIDELREAKLYLEATGLKVDAYDHEVAKSIYF
                     NDPDGNMIELYIDASDVWKQRPEAVAQGAPFDL"
     gene            5389..5901
                     /locus_tag="PLD53_02555"
     CDS             5389..5901
                     /locus_tag="PLD53_02555"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRC59816.1"
                     /translation="MNADDLRSVQAPLKERYRDTPDAALVTLHAEGRIGEGVSCKIET
                     GKALVVAGLHPATGGSGQAACSGDMLLEALVACAGVTLNAVATALGIELRDASLLAEG
                     DLDFRGTLGLSKDVPVGFQNIRLKFKLDTDASEEELATLQRLTERYCVVYQTLAHPPA
                     LSVTRKLATG"
     gene            5983..6417
                     /locus_tag="PLD53_02560"
     CDS             5983..6417
                     /locus_tag="PLD53_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59817.1"
                     /translation="MLGCPGALAQTVQQHVHQMSHSVMPFDMARTVHVFKMTETGGVQ
                     RVVAKNPDAADQIALIRQHLKHEAENFQRGNYSDPAMLHGSDMPGIKELKSGASQIKV
                     TYAAIPDGAEIVFHTSNIHLLTAIHRWFGAQLSEHGPDARAE"
     gene            6465..7001
                     /locus_tag="PLD53_02565"
     CDS             6465..7001
                     /locus_tag="PLD53_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRC59818.1"
                     /translation="MHDEILNFWFVETSPRMWWKADSAFNAAIARRFGAVHSAAALGE
                     LFEWRASPRGRLAEVIVLDQFSRNLFRKTAKAFASDPMALVLAQEAVAGSHDQGLPGQ
                     QRAFLYMPFMHSESRVIHAHAEQLFASLGLDDSYKFELRHKAIIDRFGRYPHRNMVLG
                     RESTPEELEFLQQPGSRF"
     gene            complement(7070..7378)
                     /locus_tag="PLD53_02570"
     CDS             complement(7070..7378)
                     /locus_tag="PLD53_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59819.1"
                     /translation="MSADFDSEQRILSPARRARRSLVALVIIMLLVVGSSVGSDWAQD
                     NVFLTSGPDGKSSLYYHPPFSTPGTLVALLGPTPNNLTEAGMKGVAATFESATGKQVR
                     "
     gene            8078..8587
                     /locus_tag="PLD53_02575"
     CDS             8078..8587
                     /locus_tag="PLD53_02575"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRC59820.1"
                     /translation="MSGTQHVRMLTRYKAWANEAIFSMVSKLPDEEVIRQRVTPFGNM
                     VHTLNHVYVIDAVFQAHLRGREHGYTARNTPGHPPLDELRNAVRTLDAWYVEFADALT
                     PDEVDRTIHFEFIGGGRGAMTCQEIILHVVNHASYHRGFVGDMIHQAGVTPDATDLSV
                     FIRDVVQTY"
     gene            8653..9279
                     /locus_tag="PLD53_02580"
     CDS             8653..9279
                     /locus_tag="PLD53_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRC59821.1"
                     /translation="MNKPVFYTNPQSRGRIVHWMLEEIGLPYETVWLEYGTTMKSSEY
                     LAVNPMGKVPALTHEGAIVTETAAICAYLADRFPEKNLIPPLNHADRATYFRWLFFAA
                     GPLELAVTAKTLSWQVPEGKNAFVGFGSYSETIDTLEKALTPGPYICGGQFTAADVYL
                     GAQLGWGLMFGTIEKRPLFEAYVERINARPAALQANRINEDYLKSRKS"
     gene            9393..9794
                     /locus_tag="PLD53_02585"
     CDS             9393..9794
                     /locus_tag="PLD53_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRC59822.1"
                     /translation="MKDTKALTLDDVKRIAAAAEAAAIANGWSVSIAICDAGGHALWL
                     QRMDGAPLMSAAVAPEKARTCVLTGKPSKAFEDMVNNGRYAALAMPVVPLEGGEPIVV
                     DGMIIGAVGVSGVKAGEDASVAQAGVAALNG"
     gene            10063..10446
                     /gene="apaG"
                     /locus_tag="PLD53_02590"
     CDS             10063..10446
                     /gene="apaG"
                     /locus_tag="PLD53_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRC59823.1"
                     /translation="MAESKKYQIAVSAVPQFIADQSDPTKENYVFAYTITIENVGTVP
                     AQLISRHWIITDANARVQEVRGLGVVGHQPLLQPGEKFEYTSGCQLDTPVGTMRGSYQ
                     MTAEDGTQFEAVIPEFTLAIPRVLH"
     gene            complement(10443..11681)
                     /gene="argJ"
                     /locus_tag="PLD53_02595"
     CDS             complement(10443..11681)
                     /gene="argJ"
                     /locus_tag="PLD53_02595"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRC59824.1"
                     /translation="MPVNYTTPSPETLFPVAGIRLGLAEAGIRKLDRRDLTLVALDTG
                     CTVAGVFTQNRFCAAPVLLCRSHLESGSEIRALVINTGIANAGTGESGLQAAQETCDS
                     VGRLMGVDASQVLPFSTGVILEALPVDRLIAGLPRCFGDLKADNWHAAAHGIMTTDTV
                     AKAASRQVEINGRTVTVTGISKGAGMIRPNMATMLGFVATDAGVAESLLQQLVREAAD
                     ASFNCITVDGDTSTNDSFILMATGKSGAEFADDTAPGYAELREAVTGVALELAQAIIR
                     DGEGATKFITIAVDGGKDREECKRVGYAIGHSPLVKTAFFASDPNLGRILAAIGYADI
                     AGLDVDGVRVWLGSEDGEVLVAEKGARADSYREEDGARIMKAAEITVRVDLGRGEARG
                     KVYSCDLSYEYVKINADYRS"
     gene            complement(11753..>13850)
                     /gene="secA"
                     /locus_tag="PLD53_02600"
     CDS             complement(11753..>13850)
                     /gene="secA"
                     /locus_tag="PLD53_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRC59825.1"
                     /translation="YAIVDEVDSILIDEARTPLIISGQAEDHTDLYVRMNQVAPLLVR
                     CQEENGPGDYWVDEKGHQVLMTEEGHEHAEEVLARVGLLPEGGSLYDSTNILLIHHLY
                     AALRAHNLFLKDQQYVVQNGEVIIVDEFTGRLMSGRRWSDGLHQAVEAKEGVKIQNEN
                     QTLASITFQNYFRMYGKLAGMTGTADTEAYEFQQIYGLETVVIPTNVPMRRVDNNDQI
                     FRTSKEKHEAIVADIRACQKRGQPVLVGTTSIENSELLSTLLDSEKLTHQVLNAKQHA
                     REAEIVMQAGSPGMITIATNMAGRGTDIVLGGSIEKQLAAIRDDEAMDAAAKEERIAA
                     LRSDWQKVHDQVVASGGLHIIGSERHESRRIDNQLRGRSGRQGDPGSSRFYLALDDAL
                     LRIFAGDRLKSIMERLKMPEGEAIEHPLVSRSLESAQRKVESRNFDIRKQLLEYDDVA
                     NDQRNVIYAQRNELLETDDISETITAMRQGVLYDTFRRYVPAESVEEQWDLPALEKEL
                     ASELLLQVPIVEWFRNEPTLGDEAMLERIIEQADAAYAAKVDLVGAESFHQFERNVML
                     QSLDTHWREHLAALDHLRQGIHLRGYAQKDPKQEYKREAFALFQMLLDNVRVEVNRLL
                     MTVQVRAEDVEESAPHAEVQNVKYQHADYDEALGSNDEEETRVQPIHAGPKVGRNDPC
                     PCGSGKKYKHCHGKLN"
BASE COUNT         2933 a         3950 c         3984 g         2983 t
ORIGIN      
        1 gcgtcgctag acctcacgtc cacccatttt ttcatcggga tgcgcaatga ccaccgtctt
       61 catcagctac tcgaccaagg accactactt tgccgaactc gctggtatca agcttgcgga
      121 ggcgggaatc aatctgtggc gggaccaggg gcagcttcgg ccgggcagcg actggcgcaa
      181 cggaatcgaa cgcggcatat cggaatcgat tgccgtgctt gtggcgctca gtgagaattc
      241 ggcgcagtct tcctacgtca cgttcgagtg ggcttatggg cttggcaagg gcaagaccgt
      301 aatccctgtc aagctggagg catgcgccgt tcatcctcgc ctggaaccga ttcagtcgct
      361 cgacttctcg atcccgggtt ccctgccctg gaatctcttg atcgagcgca ttcgcgaaat
      421 cgaggccgat gcgacgtcgg aagaagacct ggagacgtct gccaatacgc ccgagcctcc
      481 gcagcaggac gcgaccgtca aggcgatcct cgcgtacctc aatcaacgtg gatatcaaat
      541 ggtcagctat gagcggctgc gtcggcgaat cgatccggcg ctgaccgacc agaagctcga
      601 tgaagtggtg aatgcaaacc cgactgtctt ccgctttgcc cgtcttaagg agggcaagcg
      661 cggcatggcg aagctcatcc cataggacgg gtggtacctg cttgaagcgg gcgaacgccc
      721 tttgtcgaca acgtttcggc cccgtatgct gaccattttt acgaccttgg ctgattggat
      781 tgcttttacg ctgtttggcc ttgcgccgca ctcaaagctg ggcgatgcag ttcacttctt
      841 cattgaggat acgaccaaga tattttttct cctggttgta atgatctatt tcattgcgtt
      901 gatccgcgcg tctctaaacg tcgaaagaat ccgggattat ctggccaaaa aaaacaggct
      961 tgtcggatac ttcatgggat cgatattcgg agcagtaacg ccgttttgct cttgttcaag
     1021 cattcctgtc ttccttggct tcacatcggg tggcattccc gttggaataa caatgtcatt
     1081 tctattgacg tcgccgctga taaatgaagt ggccgtattg cttttgttga gcttggtggg
     1141 gtggaagata accattgtct atgtattggt tggattggca gtcggaatca cgggcggaat
     1201 atttcttgac cttatccatg ccgaaagact cctgcaaccc tttgcgctca aggcgtatca
     1261 atcggcgaag cccgcagttg gcgaaggcac cagcagatcg gcgctttctt tcggtgatcg
     1321 gcatgaattc gccaaacaag agttgctgga tattctgagt cgtgtctgga aatggatctt
     1381 catcggtgtc ggccttggcg cggcattgca cggctttgtg ccggttgcct ggatagaggc
     1441 gaaccttggg cgcggccaat ggtggtctgt ccccctggca gtttttctgg gtattccgct
     1501 ttatgcaaac gctaccggta tcatccccgt catggaaagc ctgattgcaa aaggcttgcc
     1561 gattgggacc acgttggcat tttgcatgag tacagtggcc gcaagttttc cggagttcat
     1621 attgttgaag caggtgatga cttggcgctt gctggcgact atttttgtgc ttctgctggc
     1681 atcctttacc attgtggggt ggactctaaa tgcgatctat tgaagccagg agatagagat
     1741 gaaggaaatt aaggtattag gcagtggttg tgccaagtgc accaagaccg ccgagctcat
     1801 ccagacggtt gccaccgaat tcgacgcctc cgtcaatgtg gtcaaagaaa ccaatccaga
     1861 agtaatgatg aactatggcg ttatgagtac tccggccgtc gttgtcgata atgtcgtagt
     1921 gcactcagga tcgattcctg ggcgaaagat ggtagaaggc tggttgaaat aggcgccaag
     1981 ttcgcccgtc gatttattcc cgccggcctg ccagccgctg tgcgtttgtc atccttgggg
     2041 gcatatcccg gcgcatccgc attcaccgtg atggcctgtt cccgcacagc ttgctggaga
     2101 ttcaccatgc aaacccttcc tcaccactac cgtgttgcgg cgaatgcagc gcccgagggc
     2161 gacgtcagct tatcgtcaga gaaactggaa acgctgccat ctgcgccacc tcaagagttc
     2221 ggtggccccg gtgaccgctg gtccccggaa acacttttgg tagcggccat cgcagattgc
     2281 ttcgttctca cttttcgggc catcgccaag gtctcgaagt tatcgtggct gtcgctgcgc
     2341 tgtgacgtcg aaggcacgct cgaacgcagc gagggaaaaa cgaagttcac gcagttcaag
     2401 gcaaatgcca cacttgacgt gcctccggat acaaacgagc aaagagcaca gcgtattctt
     2461 ggaaaagccg agacgagctg cctgatcacc aattcgctct ccgccacaac gcacctgaac
     2521 gctgtggtgg tggcgaagtc cttggcatga ctcaacccga cttggctctc gcctgaactc
     2581 gaccagccgg tcagccaagg cttctgcaat ctctgctaag ctccagatgg tcaacccggc
     2641 aacctgcaat cgggcgaggc catggcattc cacaaatcac caaaggagaa aagaatgggt
     2701 tttttcagca atatcctcgc aaagcttggc atcggtagcg acagcgctcc ggccccaacc
     2761 gccgcacctg cgacaccaac tgcaccggcg accccggccg caacatcagc tgcgcccgca
     2821 acacccgcgg tcgcgctcgt cgatgtcgtt gcgcaactgg agcagcgcgc gtcggccaac
     2881 ccacagaagc ttaactggcg gacatccatt gtcgacctgc tgaaactgct cgacatcgac
     2941 agcagcttcg cggcacgcaa ggagctggcc acggaattgg gctgcccggc tgacttgatg
     3001 ggggattccg cgaagatgaa catctggctg cacaagaccg tgctggcgcg catcgccgcc
     3061 aacggcggca atgtgcccaa ggacctcctg gactgaacca gcagcgccac cgccaagcca
     3121 agggagcaag agaacatgca gatcacggac atccttgccc agatgggcgg cctcaaatcg
     3181 atggctcagg aactgggcgt cgacgaagcc caggcggcga gcagcgccgc agcgcttgcc
     3241 ccggccatcc tcggcgggtt caagaagcag gctcagtcgc agcccacagg cctcgaaggc
     3301 ttcggcggtc tgctcgggaa acttggcggt ggcggactgc tcgacaacgt tttggcgccg
     3361 cagccgacgg acgtcagtca ggggaataac gtgctcggac agatcttcgg ctccaaggat
     3421 gtgagccgag cggttgcgca gaatgtggct gagcagtcgg ggcttgaccc gtccctgctc
     3481 aagaagatgc tgccgatgct cgcgatgctg gttactggct atctggcgaa gcagcagggt
     3541 accggagccg aggcgcagcc ctccccgagt gctgccttgg ggggtggcct ggggggcggt
     3601 ctcggcgatc tgcttggcgg cctgctgggc ggccagaatt ttggccacgc gccgcagggt
     3661 gcagcctcgt cgggactcgc ctcgatgctc gacatgaacg gtgacggcaa cgcgctggat
     3721 gacatcctgc gaatagccgg caaggcaatg cgttgatcgc cgtacccctc aacacgctga
     3781 acgtttcaaa cacaccgaga gtgcaaagat gaatgtgatc gtttccttga ttattggcgg
     3841 aatcgtcggc tggctggcga gcatcgtcat gaagaccaac gcccagatgg gcttgatcgc
     3901 caacgtgctg gtgggcgtgg tgggatcatc gctcggctac tggatcgcgg gaatgctggg
     3961 catcgcgccc acaggcatcc tgcgctttgg ggttgcggtc gggggtgcgg cgctgctcat
     4021 cttcatcctg ggcaagctcg gtatcttccg caggaaatag cgtcggccgc gccgcaaggc
     4081 acgttcgcga ataatcggga gcagactgtg ctggcctgga cctcaatggt ttgatgcctt
     4141 ggcagcaagg ccgacgggtg agccggcgct aaagtcaggg tgctgcgggc gagccatccc
     4201 agggggttgc ctcggaccac gctttgaaga gacttttcag atgaagaaac tgtcaattgc
     4261 attggttgta ttcatgatgg gatgtacaag catccctgaa gatgtgacac cggttgataa
     4321 tttcaaactg gagaagtatc tggggaaatg gtacgaaatt gcgcggctgg atcattcgtt
     4381 tgaacgaggt ttgagccagg taacagcaga atacagcttg agggatgacg gcggtgtcag
     4441 agtattaaac cggggttact ctgccaaaga tgattcacgg aaagaagctg aaggaaaagc
     4501 atatttcgtt aaaaaaccgg atcagggata cctgaaggtc tctttctttg gcccttttta
     4561 tggctcctat atcgttttcg aacttgatga gaattatcaa tactcactcg tatccggccc
     4621 tgataaatcc tacttatgga ttctggccag aagaccagaa atcggtgagg acctgaagaa
     4681 taccctcaca gcgaaagcgg cagcagcggg ttttgacacg agcaaattga tttttgtcga
     4741 tcacgacaaa tcgctcgcaa gtcagcctca ctaaaatggc tggtctgcat acgagcgcgc
     4801 gatctgacga tgtagtgcaa ccaaaccatt cccgtttcac acgaaaggcg caaacatgaa
     4861 gatccagtca cttgggcacg tagtgatcaa ggtgcgcgat caaaaacgcg ccgaagcctt
     4921 ctataacggg cttctcggaa tgccaatcgc agctcgattg ccttcgtttt caatgacctt
     4981 ctttactttg ggcaatcatc acgacttcgc cataaaggcg gtcggcgata gtgcgccgga
     5041 cgcgcccgag gattcaccag ggctcttcca cgtcgccttc aagatcggaa gccacatcga
     5101 cgaacttcgc gaggcaaagc tgtaccttga agcgacaggc ctcaaggtcg acgcgtacga
     5161 tcacgaggtg gccaaatcca tttacttcaa tgaccccgat ggcaatatga tagagctgta
     5221 tatcgacgcg tcggacgtct ggaagcagcg gccggaagct gtggcacagg gggctccatt
     5281 tgacctctag ggtgtgtgcc caatcagaca tatccgatgc ctcctgaatc gagggagccc
     5341 atttcattct ttggtcgtca caaatcaatc gtcaggaggt ctatccatat gaatgccgat
     5401 gacttgcggt ctgtccaggc tccgctgaag gaacgttatc gcgatacccc ggacgccgcg
     5461 ctggtcactc tgcacgcgga aggccgcatc ggcgaaggtg tgagctgcaa gatcgaaacc
     5521 ggcaaggcac ttgtcgtcgc tgggttgcat ccagccactg gtggaagcgg gcaggccgcg
     5581 tgctcgggcg acatgctgct cgaagcgctg gtcgcttgcg cgggggtaac gctgaacgcg
     5641 gtggctacgg cgcttggcat cgaactgcgg gatgcctcgc tactggcaga aggcgatctc
     5701 gacttccgcg gaaccttggg gctgtccaag gatgtccccg ttggtttcca gaacatacgc
     5761 ttgaagttca agctcgatac cgacgcctcg gaagaggaac tcgccaccct ccagcgcctg
     5821 acggagcggt actgtgttgt gtaccagacg ctggcgcatc caccagcgct gtccgtcacc
     5881 cgcaagctcg ccacaggctg aacgcctctg tcatcgagaa aagccattat gactcctgcc
     5941 ccgtttcacg ttcgtttggc aatcctgagc ataccccttg ccttgctcgg ctgccccggt
     6001 gcacttgctc aaaccgtaca gcaacacgta catcagatgt cacacagcgt gatgccattc
     6061 gatatggccc ggactgtgca cgtcttcaag atgacggaga ctggtggcgt gcagcgagtc
     6121 gtggcgaaaa atccagacgc tgcggatcag attgccttga tccgtcagca ccttaagcat
     6181 gaggcggaga attttcagcg cggtaactat tcggatccgg caatgcttca cggctcggac
     6241 atgcccggca ttaaagaact caaaagcggc gcctcgcaaa tcaaggttac ctacgcggcc
     6301 atccctgatg gcgcggagat tgtcttccat acctcgaaca ttcatttgct gacggcaatt
     6361 catcggtggt ttggcgctca gttgtccgaa catggccccg acgcaagagc ggaatagaat
     6421 cagcgcttat acggttccgt gggctgcatt ggggacactc gatcgtgcac gacgaaatcc
     6481 tgaatttctg gttcgttgag acgtcaccgc gcatgtggtg gaaggcagac tcggcgttca
     6541 acgccgctat cgccaggcgt tttggcgccg ttcacagcgc tgccgcactg ggcgaactgt
     6601 ttgagtggcg tgcgtcgccc cgcgggcgcc tcgccgaagt catcgtcttg gaccagttct
     6661 cgcgcaatct cttccgcaag acggcgaaag ctttcgcgag cgatccgatg gccttggtcc
     6721 tggcgcaaga ggctgtggcc ggatctcacg atcaaggact ccccggtcaa caacgagcgt
     6781 ttctgtacat gcctttcatg cacagtgagt cccgcgtcat tcacgcacac gccgaacagc
     6841 tctttgcctc cctcgggctg gacgacagct acaagttcga actcaggcac aaggcaatca
     6901 tcgaccgctt cggacgctac ccccatcgaa atatggttct cgggcgcgag tcgacaccgg
     6961 aagagttgga gttcctacag cagcccggct cacggttcta gctttcggcc acccggcgaa
     7021 cggccatgaa agtggcaagg cttgatttag ccgttggcac tgagccagat tagcgaactt
     7081 gcttgccggt agcggactca aaggttgccg caactccctt catgcctgcc tcggtcaaat
     7141 tgttcggcgt gggacccaac agggcaacca gggtaccagg cgtgctgaat ggcggatggt
     7201 aatagagact tgattttcca tccgggccgc tcgtaaggaa tacgttgtct tgcgcccaat
     7261 cgcttcccac gcttgaaccc accacgagaa gcatgatgat gaccaaggcc acgaggctac
     7321 gccgcgcgcg ccgggcgggc gacagtatgc gttgttcaga gtcgaaatca gcactcatga
     7381 ttgcgttcct gtagtgtctg tgttttggcc tggctgaccg atatggtcat tgatacatcg
     7441 ggcttcagag acgccgtagt gagtctggag ctcaaagcgc cgagcgcgaa gccgacgcaa
     7501 atagacacga tcagccggat cgtgatgcaa cgagttgtca aggggatctc cgctaaattt
     7561 ttgcgcccgg cgcttgctac aaacgcctgg acatgaacac cactctgacg attttacgaa
     7621 ccgattgtga caatctgtgt attggaccga cgtcgtcggg aaaactgctc tgtttcattg
     7681 tgttgcacaa gcacgatgtg caaatagccc ttcttgctct tttatcgcga ggtggtgcca
     7741 tgaatagcgc ggacgcgtcc gatacctggg agcgtcggaa cccctacgag aagtgccggc
     7801 cgttggagcc gtttggtcac cgcgaagttc ctctcatggc tgaaaattat tgccgcacga
     7861 aggtggctgg atgtgagatc cggtgctgga gcactgtacg cgacgattgt ggacaattgc
     7921 tcgctatcac cgaacgggtg tcgaaccctc gaacggattt ctgcaggcag ccacggcgaa
     7981 tctcggcggc caagggccgg cccccgccca cactatggga gaatgtgcgc acgcttgaat
     8041 cgattgcgac aattcctgca aaccactagg gaggctcatg tccggcacac agcatgtccg
     8101 aatgctcacg cgctacaagg catgggcgaa cgaagcaatc ttctcgatgg tcagcaaact
     8161 ccccgacgag gaagtgatcc ggcagcgtgt gacaccattc ggaaatatgg tgcacacgtt
     8221 gaaccacgtg tacgttatcg acgccgtgtt ccaggcgcac ctgcggggtc gggagcacgg
     8281 ctacaccgcg cgtaacacgc caggccatcc accgctcgac gaactccgaa acgcagtgcg
     8341 gacgcttgat gcgtggtatg tcgagttcgc cgatgcattg acgcccgatg aagtcgaccg
     8401 gacgattcac tttgaattca tcggcggcgg acgaggcgcg atgacgtgcc aggagattat
     8461 tctgcatgtc gtgaatcatg caagctacca ccgcggcttt gttggcgata tgattcatca
     8521 ggctggcgtc acgcctgatg caactgacct ttccgtgttc attcgcgatg ttgtgcaaac
     8581 gtactgacgg aaaccgcgac ggaatgcatt cgaacgttgg gctgttttgc tatcccgagg
     8641 aaagacacct tcatgaataa accggttttc tataccaacc cacaatcgcg cggacgcatc
     8701 gtgcactgga tgctcgaaga gataggcttg ccgtacgaaa ccgtctggct ggaatacggc
     8761 acgacgatga agagcagcga atatctcgca gtgaatccga tgggcaaggt tcccgccctc
     8821 acacacgaag gtgcgatagt cacggaaacg gccgcaattt gcgcctatct tgccgaccgt
     8881 tttccggaga agaatctgat acctccgttg aaccatgctg atcgcgccac gtatttccgc
     8941 tggctttttt tcgcggccgg tccgcttgag ctggccgtta ccgcgaagac cttgagctgg
     9001 caagtacccg agggaaagaa tgcttttgta ggttttggca gctacagcga aacgatcgat
     9061 acccttgaga aggcactcac gcccggacct tacatctgtg gcgggcaatt taccgcggct
     9121 gatgtctacc ttggtgcgca actcggctgg ggcctgatgt tcggcaccat cgaaaagcgc
     9181 cctttgtttg aagcctatgt tgagcgtatc aatgcgcgtc cggccgcgct acaggcaaac
     9241 cgcattaacg aagattatct gaaaagccgc aagtcctgac cgacatcggg cgagaaatcg
     9301 ttgacttgca cgggactcca gcacaatagg cgtccacggt agcttgatca tccgagcaac
     9361 agcgatcagg taagcgaaac aggaggaaag caatgaagga caccaaggca ctaacccttg
     9421 acgatgtgaa acgtatcgcc gccgctgccg aggcggcagc gattgccaac ggctggtcgg
     9481 taagtatcgc gatttgtgac gccggcggcc atgcattgtg gctgcagcgc atggatggcg
     9541 cgccgctgat gagcgccgcc gtggctccgg agaaggcgcg cacctgcgtg ctcaccggca
     9601 agcccagcaa ggccttcgaa gacatggtaa acaacggtcg ttacgccgcg ctggccatgc
     9661 cggtcgttcc cctcgagggc ggcgaaccca tcgtggtcga tggaatgatc attggcgcag
     9721 tcggcgtgtc cggtgtcaag gccggcgaag atgcgtcggt cgcccaggcc ggtgtagcgg
     9781 cattgaacgg atagttgtcc gatactgcaa ctgcttctga agttgatttc gtcacgggcc
     9841 ggccccgcgt atcggcaccc cggtttcagc gccgcacgag aattctgctt actggccatc
     9901 cagcaggaca agccgtctgc tcaagcgcgc tttgctcgct tcgggtagaa cgagtatgcc
     9961 cggcttgcac tgttagcaac aaatagaact gtccggcttg cacttgcctc ttgcgccact
    10021 ttggtgtgca atgctccatt cactcatttt cgtctgaaca ccatggccga aagcaagaaa
    10081 taccagatcg ccgtaagcgc cgttccgcaa tttattgccg atcaatccga ccccaccaaa
    10141 gaaaactacg ttttcgctta caccataacc atcgagaatg ttggcaccgt tccggcacaa
    10201 ttgatttctc gtcactggat catcaccgac gctaacgcac gggttcagga agtgcgcggc
    10261 cttggcgtag tcgggcatca gccgctgctt cagccgggag agaagtttga atacaccagt
    10321 gggtgccagc tcgatacgcc ggtcggcacc atgcgcggca gctatcaaat gacagcggaa
    10381 gacggtacgc aatttgaggc ggtgataccc gagtttacgc tggctatccc gcgtgtactg
    10441 cactagctcc ggtagtcggc gttgatcttc acgtactcgt acgacaagtc gcaactgtag
    10501 accttgccgc gagcttcgcc gcggccaagg tccacgcgaa cggtgatctc cgccgccttc
    10561 atgatgcgtg caccgtcctc ctcgcggtac gagtcggcgc gtgcgccttt ctcggcgacc
    10621 agaacctctc catcctcact acccagccag acacgaacac cgtcgacgtc aagcccggca
    10681 atgtcggcat agccaatggc ggcgagtatg cggccgagat tggggtcgga agcaaagaac
    10741 gcggttttga ccagcggcga atgaccgatc gcgtagccga cacgcttgca ttcctcgcga
    10801 tccttgccac cgtcgacggc gatggtgatg aatttggtgg cgccctcgcc atcgcgaatg
    10861 atcgcctgtg cgagttcgag cgcgaccccg gtcacagcct cgcgcagttc ggcatatccg
    10921 ggcgccgtgt catcggcaaa ctcggcaccg gattttccgg tagccatcag gatgaaggaa
    10981 tcgttggtcg atgtatcgcc atcgacggta atgcagttga aggacgcgtc agccgcttcg
    11041 cgaaccagtt gctgtagcag cgattcggcg acgccggcat ctgtcgccac gaaaccgagc
    11101 atggtcgcca tgttgggcct gatcatgccg gctcccttgc tgatgccggt gacggtgacg
    11161 gttctgccat tgatttcgac ctgccgggag gccgccttgg ccaccgtatc ggtggtcatg
    11221 atgccgtgtg cagcggcgtg ccagttatcg gccttgagat ccccgaagca acgtggcagc
    11281 ccggcgatca gccggtcgac gggcagtgct tcgagaatca cgccagtcga gaagggcaac
    11341 acctgactgg cgtcaacgcc cataagccgt ccgactgaat cgcaagtttc ctgcgcggcc
    11401 tgcaggccgg actcgcctgt ccccgcattg gcaataccgg tattgatgac cagcgcacga
    11461 atctcgctgc cggattcgag gtgactgcgg caaagcagaa ccggcgctgc gcaaaagcgg
    11521 ttttgcgtga acactccggc caccgtacac cccgtatcca gcgcaacaag cgtcaaatca
    11581 cgccgatcga gcttgcggat acctgcttcg gcgagtccaa gacgaatgcc agcaacggga
    11641 aaaagggttt cgggtgaggg ggtggtgtag ttgacgggca taaacatcct tgaacgctaa
    11701 gtgaacgtta ggctgagccg gaacaagacg ccaggccagc cggcaagctc cttcagttca
    11761 atttgccgtg acagtgcttg tattttttgc ccgagccaca gggacagggg tcatttcgcc
    11821 cgactttcgg tccggcgtga atcggttgaa ccctggtttc ttcctcgtcg ttcgaaccga
    11881 gcgcctcatc gtagtcggca tgctggtact tcacgttctg cacctcagcg tgcggtgccg
    11941 actcttcgac gtcttccgcg cgaacctgga cagtcatcaa cagccgattg acttcgaccc
    12001 gcacgttgtc aagcaacatc tgaaagagag cgaaggcttc acgcttgtat tcctgctttg
    12061 gatccttttg cgcgtaacca cgcaggtgga tgccctgccg caggtgatcg agcgcggcga
    12121 ggtgttcgcg ccagtgggtg tccagactct gcaacatcac attgcgttcg aactgatgga
    12181 aactctcagc gccgaccaga tcgacttttg ccgcgtaagc cgcgtcggcc tgctcaatga
    12241 tgcgctcaag catcgcctca tcacccagag tcggctcatt tcggaaccac tcgacgatcg
    12301 gcacctgcaa cagcagctcg gaagccagtt ctttttccaa tgccggcagg tcccattgtt
    12361 cttcaacgct ttccgcggga acataacgcc tgaacgtgtc gtacaggaca ccctggcgca
    12421 tggcggtgat ggtctcggat atatcatcgg tttcgagcag ctcgttgcgc tgcgcataga
    12481 tgacattgcg ctgatcgttg gccacatcat catattcaag caattgcttg cggatatcga
    12541 agttgcggct ttcaaccttg cgctgggccg attcgagcga gcgcgacacc agcggatgct
    12601 cgatcgcctc gccttccggc atcttcaggc gctccatgat cgacttcagt cggtcgccgg
    12661 caaaaatccg tagcaatgcg tcgtccagtg cgagatagaa acgcgatgag cccggatcgc
    12721 cctgacgacc ggaacggccg cgcaattggt tatcgatccg gcgcgattca tggcgctcgg
    12781 aaccgatgat gtgcagcccg ccggaagcga ccacctgatc gtgcactttc tgccagtcgc
    12841 tgcgtagcgc ggcaatcctt tcctctttgg cggcggcatc catcgcctcg tcatcacgta
    12901 tcgccgcgag ctgcttctcg atactcccac ccagcacgat gtcggtgccg cgaccggcca
    12961 tgttggtcgc aatggtgatc attcccggac tgcccgcttg catgacaatc tcggcttcgc
    13021 gggcgtgctg cttggcattg agcacttgat gagtcagttt ttcgctatcg agcagcgtcg
    13081 aaaggagttc ggaattttcg atcgacgttg tacccaccag caccggctga ccacgcttct
    13141 ggcaagcgcg aatatcggca acgatagcct cgtgcttttc cttggaggta cggaagatct
    13201 ggtcgttgtt gtcaacacgc ctcatcggca cgttggtcgg gataactact gtttcgagac
    13261 cgtatatttg ctggaactcg taagcctcgg tatctgcggt accggtcatt ccggcgagct
    13321 tgccatacat gcggaagtag ttctgaaagg tgatcgaggc cagcgtctgg ttctcgttct
    13381 gtattttgac cccttccttc gcctcaaccg cctggtgcaa gccgtcagac caacggcgtc
    13441 cggacatcag gcgcccggtg aattcgtcaa cgatgatgac ttcaccgttc tgcaccacgt
    13501 attgctgatc cttaaggaac aggttatgtg cgcgcaaggc ggcgtaaaga tggtgtatca
    13561 gcaggatgtt ggtcgaatcg tacagactgc cgccttcggg cagcaaaccg acgcgggcga
    13621 gaacttcttc ggcgtgctcg tgcccttcct cggtcatcag gacctgatgg cccttttcgt
    13681 cgacccagta gtcaccggga ccgttctctt cctgacagcg caccagcagg ggtgctacct
    13741 gattcatacg cacgtagaga tccgtgtgat cctctgcctg accggaaatg atcaacggag
    13801 tgcgcgcctc gtcgatcagg atcgagtcga cctcgtcgac gatggcatag
//
LOCUS       DAORPM010000029        13565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_925_length_13565_cov_11.053220, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000029 DAORPM010000000
VERSION     DAORPM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 13565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13565
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_925_length_13565_cov_11.053220"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1875
                     /locus_tag="PLD53_02605"
     CDS             <1..1875
                     /locus_tag="PLD53_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59826.1"
                     /translation="TAAAPDYFLFPRVVVAVDSPPSVPSGLLLKDRLYIGYQEKSDVL
                     EVISYNESAGRFEFQLVKNYRAGAKPQVFYANRTLCYACHQNGAPIFSRALWDETNAN
                     PKVAALLSASGKDFYGIPPERGVDIPYAIDIAIKRANGFALTQRLWQEGCGGADRPAL
                     RCRAGLFTAALRHALSDGRKGTVAPAQSDSNSTLFRAEADRRWPGGLAIGASDIPNRN
                     PLQDLGSGPDDIARRIGFSHVPALFEPLTRRSSQEIWRADTADAIDRITGGLAEFVSA
                     SDRQRLVDALAARPAQSIQYHAPCRMTPGASRWSVGCQPGDGNSGLKLTATLDKTRAH
                     GRVETRNQSPGGRLERLTLPASGAFNSIALMSSAPRAGERDTFTPDNKPLPRGADGNP
                     LVRIAFQVSPGKPDDGEVLVEMREEFPAVEQAVTALAEGPDGPALFGPRPFPREQLFA
                     ALLARLGAPVVTPCCQTADKLPPPQLEVTAIAPSSPALIPVAPVLQGFYPYCATCHQS
                     AETFPPNFLTGTATQVEAQLRQCAPRLYVRLSMADQTPEHRNKTPMPPESLLPAFGTD
                     IAGWRASPARAALLAQVGNWLRAETGKTPDLTLLLAGGYEALRPCLPTQRPATNPSPR
                     "
     gene            1881..3407
                     /locus_tag="PLD53_02610"
     CDS             1881..3407
                     /locus_tag="PLD53_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRC59827.1"
                     /translation="MQDKNMPSDPAENPWKRRFVILVIVTVGIPAIVGLWLLQRFGGD
                     MPVDYKSPTEHFKYGSTGGEHEMGFPYWIWKALPEVCPQYLPGKGYQSLGMVYEKQPD
                     GSDKDLPIGTSKRRFQGIDRVFVNCAACHVSTVRTAADTAPTIVLGMPAATFNMKGFE
                     EFFFRCAADPKFSKEFILPEIERQGANLDLLDRYLVYPVAIAIMRDRVLALAGRFDWV
                     FKQKEWGPGRVDTFNSAKVIFNFPMAHLDPKEFDAPADFPSIWRQRQRKEPKEMQLHW
                     DGNNTTVEERNKSAAFGTGTTPPTIDIARIRRVEDWIMDVTPPPFSDFFPIDDKLAAK
                     GAPIYKEYCAACHGASGSDFSGEHVGQVVPLAKIGTDRRRLDSYTYTLAVNQATLYAG
                     YPWRFTHFQKTHGYANMPLDGLWLRAPYLHNGSVPSVRDLLEPAAKRPKSFFRGNDVY
                     DPARLGFVSNVPEANGKTFFRLDTAQPGNGNGGHEGKAYGTELPLAEKTALVEFLKTF
                     "
     gene            3421..4896
                     /locus_tag="PLD53_02615"
     CDS             3421..4896
                     /locus_tag="PLD53_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRC59828.1"
                     /translation="MITTKCKTWLKIGGLALVVVGLLGAMIGYDRGFREHPQPDWVTA
                     TPEMRFKYGSIGAENDAGIPYWIFYVLPRVFPEKLTQDGKVIPGGYAALGVPWEQGRE
                     LPTGFTKKKIGFDRVANNCAVCHTTSYRESPDANPVFVVGGSSHTTNVESFFRYLVDC
                     AKDPRFNADILMAEINRVTNLDFIDKLLYRFFIIPITKKRLIEREEQFAWIYREDFPE
                     WGRGRDDAMNLTKYFMIKAPMDDTFGPTDMPSVWNLKKYVWDKGHRMNYAGDSHDPYS
                     VIMDSALGLLGAAPADKADFVGQVQWLKDYLSELPPPKYPFPIDNAKAAAGKPLFDAH
                     CASCHASDKTGTPLLLAEVGTDRGRLDSWNKGAAIKANQVVKDMGLERRGLVEEDLKG
                     YVAAFLDGIWLKAPYLHNGSVPTLRDLLEPVAKRPKVFWRGYDVYDQTKVGFVTDTPE
                     AQRVGTRLDTASKGGSNQGHEFGTGLSATDKAALVEYLKTL"
     gene            complement(4945..6804)
                     /locus_tag="PLD53_02620"
     CDS             complement(4945..6804)
                     /locus_tag="PLD53_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRC59829.1"
                     /translation="MHSRQAVPALTVGAIGVVFGDIGTSPLYAMKEIFNGHHPIPVTP
                     ENILGILSMVFWSIMVMVSIKYVAIIMRADNRGEGGSLALLALITDRAKSSGLTWIVT
                     LLGIFAAALFYGDSMITPAISVLSAVEGLEVIAPSLKPYVIPVTLGILTGLFVIQKRG
                     TGAVGMLFGPIMILWFALLGVLGAMEVAHNPGVLAALNPLYAINFINTHSGLAFLALG
                     SVVLAVTGGEALYTDMGHFGPFPIRLAWFAFALPTLVLNYFGQGALLLSEPQAIASPF
                     YHLAPDWALVPLVTMATVATVIASQAVISGAFSVARQAIQMGFLPRMLIVHTSGKAKG
                     QIYVPFTNWTLYLAVVALVIGFGNSSNLAAAYGIAVTGTMLIDTVLIAFVMVLMWRWH
                     WSIVALVAGSLLIFDLAFFSSNILKIPAGGWFPILIGLASFTVLTTWRRGRKLVGEEM
                     AKQSIPIHAFLDSIFDVHRVHGTAVFMTSTKEGVPAALLHNLKHNQVLHERVVLVTVQ
                     TTEAPNVLEHDRIYLHQMQQGFIRLIIRYGFMEDPDIPGALMSCKRFGESFDLMETTF
                     YLSRETIVPTLTRNMTCLRARLFALMSKNATSATEFFKIPTGRVVELGTQLEI"
     gene            complement(6957..7373)
                     /gene="arfB"
                     /locus_tag="PLD53_02625"
     CDS             complement(6957..7373)
                     /gene="arfB"
                     /locus_tag="PLD53_02625"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRC59830.1"
                     /translation="MSVQSQQILLREEEVEFIAIRAQGAGGQNVNKVSNAIHLRFDIG
                     ASSLPEEIKNQLLQMRDQRISADGVVVIKAQAHRSLERNRIDSLTRLRELIARAAFVP
                     VVRRPTRPTRSSQKKRIENKVRRGLVKVLRGRVETD"
     gene            complement(7438..8394)
                     /locus_tag="PLD53_02630"
     CDS             complement(7438..8394)
                     /locus_tag="PLD53_02630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRC59831.1"
                     /translation="MRAVAVLLGDGADVGILSRDAQGETVLRVFSGVTIMNSAVFLWP
                     EFLQDVRSDGKLVTRCIRGRNCGALMLQSSELGSEQTILAYDVNQLDAAEHAEEIASL
                     ARMYGNQIKLLDYSELDSLTRLLNRKTFDETFDRLLTVSSTDMPDDDFDDRRSSPDMG
                     SQAWLCVVDVDHFKRVNDSFGHLFGDEVLLRMGKLMRKTFRGADRLFRFGGEEFVVIL
                     NAETEALASTGFNRFRESVESHEFPQVGKVTCSIGFTAVSNIDVPTDVVGRADEALYY
                     AKEHGRNQVCCYESLVAAGAIAKAKAVEAAVDEDFDIDALFG"
     gene            8591..9046
                     /locus_tag="PLD53_02635"
     CDS             8591..9046
                     /locus_tag="PLD53_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRC59832.1"
                     /translation="MRLVLDTNIVMDMLHFADRHTHALRIALNHGHMTCFTDSECLAE
                     LERVSRYPEFGLDLPARNALIEGYRGIATLCDASGVEDYLLPRCRDRDDQKFLVLAAR
                     SKADLLITRDKMLLKLVRNRRNPPPCPIVTAETACTLLRLAPENPAGFV"
     gene            complement(9005..9778)
                     /gene="yaaA"
                     /locus_tag="PLD53_02640"
     CDS             complement(9005..9778)
                     /gene="yaaA"
                     /locus_tag="PLD53_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HRC59833.1"
                     /translation="MILVLSPAKALDFTTPATIADHTQPDFLDQSQLLIKRLRTLSPL
                     EIEKLMGVSSALAVLNASRYSAWDQPFTPENAKHAVLAFNGDVYEGLDAKTLSPADLH
                     FAQGHLRILSGLYGVLRPLDLMQAYRLEMGTRLSSRRGKDLYAFWGNRIVDSLNSALR
                     DVEAPALVNLASAEYFRAVKLRRLAVPVIQPVFEDWSAGRYRVVSFFAKRARGLMARF
                     AVTGRLLQPKDLQAFADEGYSYTAMASDETRWVFRRKPQ"
     gene            complement(9818..10261)
                     /gene="queD"
                     /locus_tag="PLD53_02645"
     CDS             complement(9818..10261)
                     /gene="queD"
                     /locus_tag="PLD53_02645"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRC59834.1"
                     /translation="MLITRRLEFDAGHRIPDHKSQCRHLHGHRYALEITLSGDIIVQA
                     GDPANGMVMDFSEVKSLAKEHLIDHWDHSFLVYAGDKQLVEFLNSIPDHKTVILDCIP
                     TSENLAVQAFSILDSVFQDTYGNHLRLERVRLYETPNCWADAGRQ"
     gene            10370..11203
                     /locus_tag="PLD53_02650"
     CDS             10370..11203
                     /locus_tag="PLD53_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC59835.1"
                     /translation="MLNVRNHSRDSAGMTYVYPVLSRRACGLSLGINLNVNNACNWAC
                     VYCQVPNLTRGGPPPVNLDLLEDELRRFLNGITTGDSLRRLAPPEAQQLVDVAFSGNG
                     EPTSAPEFAKAVERVGRVLQECGLLESVKLRLITNGSLMHRPVVRQGIAAIGALNGEI
                     WFKLDRATAIGIERVNGIHTTPEKVRRSLLQCAELAPTWVQTCYFAIDGDEASEAERS
                     GFREFMKSVAGKIKGVHLYGLARPSMQPDAGRLSNLSKEDFQRFAGSISALGIEVVAN
                     L"
     gene            complement(11215..11517)
                     /locus_tag="PLD53_02655"
     CDS             complement(11215..11517)
                     /locus_tag="PLD53_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRC59836.1"
                     /translation="MNTSKSTDKSEPREIRRKLGLNQQQFWSKIGVTQSGGSRYESGR
                     NMPRPVRELLRLVHVEQIDIQRIKREDMDVIEYLKSEESELYKTLKKSAKAKKKTG"
     gene            11767..12993
                     /locus_tag="PLD53_02660"
     CDS             11767..12993
                     /locus_tag="PLD53_02660"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRC59837.1"
                     /translation="MALIVQKFGGTSVASTDRIKSVARRVARWKAQGNDVIVVPSAMS
                     GETNRLIGLAREISAEPDPRELDVIASTGEQVTIALLAMAMHEEGLKAKSYTGAQVRV
                     LTDSTFTKARILQIDDEKIRKDLAEGNVVVVAGFQGADENGNITTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARKLDTITFEEMLEMASLGSKVLQIRSVEF
                     AGKYKVKLRVLSSFDDEGEGTLITVEEEKKMEQPIISGIAFNRDEAKLTVLGVPDRPG
                     IAYQILGPIAEANIDVDMIIQNVGHDGTTDFSFTVNRGDYTKALGVLETVKTKLDARG
                     VVGDNKTCKVSAVGVGMRSHPGVASQMFSALASESINIQMISTSEIKISVVIEEKYLE
                     LAVRVLHKAFGLDKAD"
     gene            13048..13140
                     /locus_tag="PLD53_02665"
     tRNA            13048..13140
                     /locus_tag="PLD53_02665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13082..13084,aa:Ser,seq:gct)
BASE COUNT         3042 a         4023 c         3782 g         2718 t
ORIGIN      
        1 accgcggcag cgcccgatta cttcctgttt ccgcgcgtgg tggttgccgt ggattcaccg
       61 ccgtccgtgc cctcaggact gttgctgaaa gaccgtctgt acattggcta ccaggaaaaa
      121 tcagacgtgc tggaagtcat cagctacaac gaatcggccg gtcgatttga gttccagctg
      181 gtcaagaatt accgggctgg cgccaagcct caggtcttct acgccaatcg tacgctctgt
      241 tatgcctgcc accagaatgg cgcacccata ttttcccgcg ccttgtggga cgaaacaaac
      301 gccaacccca aagtcgccgc attgttgtcc gccagtggca aagatttcta cggaataccc
      361 ccggaacggg gcgttgatat cccctacgcc atcgacatcg cgatcaagcg tgccaacggc
      421 tttgcgctga ctcagcgtct ttggcaagag ggttgcggcg gggcggaccg gccggccttg
      481 cgctgtcgtg ccggcctgtt caccgctgcg cttcgccatg ccctctccga tggtcgcaag
      541 ggaacagttg cccccgccca aagcgacagc aactccactt tgtttcgtgc cgaagccgat
      601 cgacgctggc ccggcggact ggccatcggc gcgtcggaca tccccaatcg caatcccctg
      661 caggatttag gttccgggcc cgacgacatc gcccggcgca taggcttttc gcacgtcccg
      721 gcgctcttcg aaccgctaac ccggcgctcg tcccaggaaa tctggcgagc cgacacggct
      781 gacgccatcg accgaataac cggcgggctg gccgaattcg tttctgcctc agaccggcag
      841 cgcctcgttg acgcccttgc ggctcggccg gcacaaagca tccagtacca tgccccctgc
      901 cgtatgacgc caggcgcttc gcgctggtca gtaggctgcc agcctggcga cggcaatagt
      961 ggattgaaac tgacggcgac cttggacaag acacgcgctc acggtcgagt cgaaacccgt
     1021 aatcagtcgc cgggtggccg actggaacgc ctgaccctgc ctgcgagcgg tgctttcaat
     1081 tcaattgcac tgatgagttc ggcgccccgt gcaggcgaac gcgatacctt cactccggat
     1141 aacaaacccc tgcctcgggg ggccgatggc aacccgctgg tacgcatcgc cttccaggtc
     1201 tcaccgggga aaccggacga tggggaagtg cttgtcgaaa tgcgcgaaga gttccccgct
     1261 gtagagcagg ccgtgaccgc cctcgccgaa ggcccggatg gcccggcgct tttcggtcct
     1321 cgccccttcc cgcgggaaca gcttttcgct gcactgctcg ccaggcttgg tgcgcccgtc
     1381 gtaacaccct gctgccagac ggccgacaaa cttccaccac cgcagctcga agttaccgcc
     1441 atcgcgccaa gcagcccggc gcttattccg gttgcccctg tcctgcaggg cttctatccc
     1501 tattgcgcca cctgccacca gagcgccgag actttcccgc ccaatttcct gacgggtacc
     1561 gccacccagg tggaagctca gctacgacag tgtgcaccac gactttatgt gcgactgtcc
     1621 atggccgatc agacgcccga acaccggaac aagaccccca tgccgccgga gagcctgttg
     1681 ccagcctttg gcaccgatat cgccggctgg cgtgccagcc cggcccgcgc cgcactgctc
     1741 gctcaggttg gtaactggtt gcgcgccgaa accggcaaga caccggacct gacacttctt
     1801 ctggccggtg gctacgaagc cctgcgtccc tgcctgccca cccagcgccc tgcgacaaac
     1861 ccatcaccaa ggtgaacgcc atgcaagaca aaaacatgcc ttccgatccc gcggagaacc
     1921 cctggaaacg acgtttcgtg attctcgtca tcgtcaccgt tgggattccg gcaatcgtcg
     1981 gactgtggct gctccagcgc ttcggcggcg atatgccggt cgactacaag agcccaaccg
     2041 aacatttcaa gtacggctcc accggcggcg agcatgaaat gggcttcccc tactggatat
     2101 ggaaggcgct gcccgaggtt tgccctcagt atctgccggg caaagggtat cagtcgctgg
     2161 gcatggtcta tgaaaaacag cctgacggta gcgacaagga tctgcctatt ggcacctcga
     2221 aacgccgctt ccagggtatc gaccgggttt tcgtcaattg cgccgcctgc catgtcagca
     2281 ccgtgcgcac cgccgccgac acggcgccga ccatcgtcct cggcatgcct gccgcgacct
     2341 tcaacatgaa ggggttcgag gaattcttct tccgctgtgc tgccgatccg aaattctcca
     2401 aggaattcat ccttcccgag atcgagcgcc agggtgccaa ccttgatctg ctcgaccgtt
     2461 acctggtcta tccggtggcc atcgccatca tgcgcgaccg ggtgctggcc ctggccgggc
     2521 gcttcgactg ggtcttcaag cagaaggagt gggggccggg ccgggtcgac accttcaatt
     2581 cggccaaagt catcttcaac ttcccgatgg cccatctcga tccaaaggaa ttcgacgcac
     2641 ctgccgactt cccctcgatc tggcgtcagc gccagcgcaa ggagccgaag gaaatgcagt
     2701 tacactggga cggtaacaac accaccgtcg aggagcgtaa caagagcgcc gccttcggta
     2761 ccggcacaac accgccgacc atcgacatcg cccgcatccg ccgtgtcgaa gactggatca
     2821 tggatgttac gccaccgccc ttcagcgact tcttcccgat cgacgacaag ctcgccgcca
     2881 aaggagcgcc catctacaag gaatattgcg ccgcctgcca tggcgcgtcg ggaagcgatt
     2941 tttctggcga gcatgtcggc caggtggtgc cgttggccaa aatcggcacg gatcgccgcc
     3001 gtctggattc ctacacctac acgctggcgg tgaaccaggc caccctctat gccgggtacc
     3061 cttggcgttt cacgcatttc cagaagaccc acggctacgc caacatgccg ctcgacggcc
     3121 tgtggttgcg cgcaccctac ctgcacaacg gctcggtccc cagcgtacgc gatctgctcg
     3181 aaccggccgc aaaacggccc aagtcgttct tccgtggaaa cgacgtctat gatccggcaa
     3241 gactcggctt cgtctccaac gtaccggaag ccaacggcaa gactttcttc cgcctcgaca
     3301 ccgcgcaacc cggcaacggc aatggcggcc acgagggcaa ggcctacggc accgagctgc
     3361 ccctcgccga gaagacggcg ctggtcgagt tcctgaaaac cttctgaagg cgatgacatc
     3421 atgattacca cgaaatgcaa gacctggctc aagatcggag gcttggcgct ggtggttgtc
     3481 ggcctgcttg gcgcgatgat cggctacgac cgaggcttcc gcgagcatcc gcaaccggat
     3541 tgggtcacgg ccacgccgga aatgcgcttc aagtacggtt ccatcggcgc cgaaaacgac
     3601 gcgggaattc cctactggat cttctatgtg ttgccgcgcg tctttccgga aaagctgact
     3661 caggacggga aagtaatccc cggaggctat gcggcgctcg gtgtgccctg ggagcagggc
     3721 cgtgaactgc cgacggggtt caccaaaaag aaaatcggct tcgaccgcgt ggccaacaac
     3781 tgcgccgtct gccacacgac cagctaccgc gagtcacccg atgccaaccc ggtgttcgtg
     3841 gtcggcggct catcgcatac aaccaacgtt gaaagcttct tccgctacct cgtcgactgc
     3901 gccaaggatc cgcgtttcaa cgccgacatt ctgatggccg agatcaatcg ggtcaccaat
     3961 ctcgatttca tcgacaagct gctgtatcgc ttcttcatca ttcccatcac caagaaacgc
     4021 ctgatcgaac gtgaagagca gttcgcctgg atctaccgcg aagatttccc ggaatgggga
     4081 cgcggccgcg atgacgccat gaacctgaca aagtacttca tgatcaaggc accgatggac
     4141 gacaccttcg gccctaccga catgccctcg gtctggaatc tgaaaaaata cgtatgggac
     4201 aagggccacc gcatgaatta cgccggcgac agccacgatc cctactcggt gatcatggat
     4261 tccgcactcg gcctgctcgg cgcggctccg gccgacaagg cggattttgt cggacaggtg
     4321 caatggctga aggactatct ctccgagcta ccgccgccaa aatatccctt ccctatcgat
     4381 aacgccaagg cggcggctgg caagcctctg ttcgacgctc actgcgccag ctgccacgcc
     4441 agcgataaga ccggcacgcc cctacttctg gccgaggtcg gcaccgaccg aggtcgcctc
     4501 gactcgtgga acaagggcgc cgccatcaag gccaatcagg tagtcaagga catggggctg
     4561 gaacgccgcg gtctggtcga agaggacctg aaaggctatg tcgccgcttt tcttgacgga
     4621 atctggctca aggcacccta cctgcacaac ggctcggtgc ccactttgcg cgatctgctg
     4681 gaaccggtcg ccaaacgccc caaggtattc tggcgcgggt acgatgtcta cgatcagacc
     4741 aaggttggtt tcgtcactga cacgcccgaa gcccaacgcg ttggaacacg cctggatacc
     4801 gcaagcaagg gtggcagcaa ccaggggcac gagtttggaa ccggtttgtc ggccacggac
     4861 aaagcggctc tggtggaata tctgaaaacg ctgtaggaga tccttgcgcg catgaagatg
     4921 cgcgcaagag tgcacgcaag atcgctagat ttcaagctgg gtacccaact cgacgacccg
     4981 acccgttgga atcttgaaaa actcggtcgc actggtcgcg tttttcgaca ttagcgcgaa
     5041 caagcgggcg cgcaggcacg tcatgttgcg agtcaatgtc ggaacgatgg tttcacgcga
     5101 cagatagaac gtcgtttcca tcaagtcgaa actctcgccg aagcgtttgc agctcatcag
     5161 agcgccggga atatcggggt cttccatgaa gccataacgg atgatcaaac ggatgaagcc
     5221 ttgctgcatc tggtgtagat agatgcgatc gtgttcgaga acattgggtg cttcggtcgt
     5281 ttgaacggta accaggacca cccgctcgtg cagaacctga ttgtgcttga ggttgtgcaa
     5341 cagtgccgca ggcacgcctt ccttggtact ggtcatgaaa actgccgtac catggacacg
     5401 atgcacatcg aaaatcgaat caaggaaggc atgtattgga atgctctgct tggccatttc
     5461 ctcgccgacc agcttgcggc cacgccgcca ggtggtcaat acggtgaatg acgccagtcc
     5521 gatcaggatc ggaaaccagc caccagccgg gatctttagg atattggacg agaagaaggc
     5581 aagatcgaag atgagcagtg agccagcaac aagcgcaacg atactccagt gccagcgcca
     5641 catcagcacc atcacaaacg cgatcagcac ggtgtcgatc agcatcgtcc cggtcaccgc
     5701 aatgccgtag gcggcggcca gattggatga attcccgaac ccgatgacca gggcgacgac
     5761 agccagatag agcgtccagt tagtgaatgg cacataaatc tggcccttgg ccttgccgga
     5821 tgtatgaacg atcagcatgc gcggcaagaa tcccatctga atggcctggc gcgccactga
     5881 aaacgcgcct gagatcacgg cctgggatgc gatgaccgtg gcaaccgtag ccatggtgac
     5941 caggggaacc agagcccagt ccggcgccag atggtagaac gggctcgcaa tggcttgcgg
     6001 ttcgctgagc agcagtgcgc cctggccgaa gtaattgagc accagtgtag gcagggcaaa
     6061 agcgaaccac gccaggcgaa tggggaacgg tccgaaatga cccatgtcgg tataaagcgc
     6121 ttcgccaccc gtgacggcca gcaccactga acccaaggcc agaaaggcga gcccggaatg
     6181 ggtatttatg aagttgatgg cgtaaagggg gttcagcgca gcaagcacgc cggggttatg
     6241 agccacctcc atggccccaa gaacgccgag gagtgcaaac cagagaatca tgatcggccc
     6301 aaacagcatg ccgacggctc cggtgccccg tttctgaatc acgaaaagcc cggtcaggat
     6361 gcccagtgtt acgggaatga cgtaaggctt cagagacggc gcgatgactt ccagcccttc
     6421 aacggccgag aggacggaaa tggccggtgt gatcatgctg tcgccgtaaa acagggcagc
     6481 cgcaaagatt ccgagcaggg taacgatcca cgtcaatccg gaactcttgg cgcgatcggt
     6541 gatcaaggca agcaatgcca gcgaaccgcc ctctccgcga ttgtccgcac gcatgattat
     6601 tgcgacgtac ttgatcgaca ccatcaccat gatggaccag aacaccatcg agagaatgcc
     6661 gagaatgttt tcgggcgtca ccggaatcgg atgatggccg ttgaaaatct ccttcatcgc
     6721 ataaagggga ctggtgccga tgtcaccaaa aacgacgccg atagcgccga cagtcagagc
     6781 aggcactgct tggcgagaat gcataactcc ctcgattgcg ccttgggcgc ttatgattgt
     6841 gattgatcga ttcgcctgtt gatgttcagg ttttcgacct gatcactaca ggcaacttac
     6901 aattggcaga attcgtgatt gtatccagag catggacaga ttgcccagga tatcggctag
     6961 tcggtttcaa cccggccgcg caataccttg accagcccgc gcctgacttt gttttctata
     7021 cgttttttct gtgaactacg cgtcggtcgg gtcggacgcc gcacaacggg cacaaaggcc
     7081 gcacgggcaa tcagctcgcg caatcgagtc aaactgtcga tgcgatttcg ttccagactg
     7141 cgatgcgcct gcgccttgat gaccacgacg ccatctgcgc tgatacgctg gtcgcgcatc
     7201 tgcaagagtt gattcttgat ttcttcaggc agcgaggagg ctccgatgtc aaagcgcagg
     7261 tggatggcgt tcgacacttt gttgacgttc tgaccgccgg caccttgtgc ccgaatggca
     7321 atgaactcga cctcttcctc gcgcagcagg atttgttggg actgaacaga catatgttgg
     7381 cgacttctct ccgctcccta ctgcaccctc aaattgaaca agcgcaatcc cgacaggtca
     7441 cccgaaaaga gcgtcaatat cgaagtcttc atcgaccgcc gcttcaaccg cctttgcttt
     7501 ggcaattgcg cctgcggcaa cgaggctttc ataacaacag acctgattcc ggccatgttc
     7561 cttcgcgtag tacaacgcct cgtcggcacg accaacaaca tcggtgggca cgtcgatgtt
     7621 cgaaacggcg gtaaacccga ttgaacaggt gacctttccg acctggggga attcatggct
     7681 ttcgaccgat tcacggaagc ggttgaatcc ggtcgatgcc agggcttcgg tttccgcgtt
     7741 cagaattacg acgaattctt caccaccaaa ccgaaacagc cggtcagcac ctcggaaggt
     7801 cttccgcatg agcttgccca ttcgcaacag aacctcgtca ccaaagaggt ggccaaacga
     7861 atcgttcacc cgcttgaagt gatcgacatc cacgacacac agccacgcct gcgaacccat
     7921 atcaggactc gaacgtcgat cgtcgaagtc atcatcgggc atatcggtgg aagagacggt
     7981 caacagccgg tcgaacgtct cgtcgaaggt cttgcgattg agcagtcggg tcagcgaatc
     8041 cagttcgctg taatcgagaa gcttgatctg attgccatac attcgcgcca ggctggcgat
     8101 ttcttcggca tgctcagcgg catcaagctg atttacgtca tacgccagga tcgtctgctc
     8161 ggagccgagc tcggaggatt gcagcatcag ggcaccgcag ttccggccgc gaatgcaacg
     8221 ggtcaccagc ttcccgtcgc tcctgacatc ctgaaggaac tcgggccata gaaaaacggc
     8281 cgaattcatt atggtcaccc ctgaaaacac tctcaggaca gtttcgccct gcgcatcgcg
     8341 cgaaagaatt ccgacatccg ccccatcgcc aagcaggact gccaccgcac gcatcaaggc
     8401 tcggggcaat ctggcccgat ccctgcaata cgtaatttct tcaataaagc gtaacagggg
     8461 caacacgatt ttgaattttc acagtaggcc cgatgggcga gtagtacaga gcaacggaaa
     8521 caatcagaac gattctatgt gcgtatgatg cgctgcacaa ctccaaccag catattttcg
     8581 gatattccca atgcgccttg tgctagatac caacatcgta atggacatgt tgcacttcgc
     8641 cgaccgccac acccacgccc tccgaatagc gctcaatcat gggcacatga cgtgtttcac
     8701 cgatagcgaa tgcctggcag aactggaaag agtaagccgc tacccggagt tcgggctcga
     8761 tttgcccgca cgcaatgccc tgatagaagg ctaccgagga atcgccaccc tgtgcgacgc
     8821 cagcggagtc gaggactacc tgctgccacg ctgtcgcgac cgggacgatc agaaattcct
     8881 ggttcttgcc gcccgcagca aggccgacct gctcataacc cgtgacaaaa tgctgctcaa
     8941 actggttcgc aacaggcgaa atccgccgcc ctgccccatc gtcacggccg aaaccgcctg
     9001 caccttactg cggcttgcgc cggaaaaccc agcgggtttc gtctgaagcc atcgccgtgt
     9061 aggaatagcc ttcgtcagcg aacgcttgca gatctttcgg ttgcaataat cgtccagtga
     9121 cggcaaaacg cgccatcagc ccgcgggcac gcttggcgaa aaagctgaca accctgtacc
     9181 ttcccgcact ccaatcttcg aatacgggct ggattactgg cacggcaagt ctccgcaatt
     9241 tgaccgctct gaagtactcg gccgatgcca gattgaccag cgccggcgcc tcgacatccc
     9301 gcagcgcaga attcagcgaa tccacgatgc gattgcccca aaaggcgtac aggtccttgc
     9361 cgcgtctact cgacagccgt gttcccattt ccagacgata ggcttgcatc agatcgaggg
     9421 gacgcagcac accgtacagc ccggagagaa ttcgcaaatg gccctgcgca aaatgaaggt
     9481 ccgcgggaga aagcgtcttg gcatccaggc cctcatagac atcgccgtta aaggctagta
     9541 ctgcgtgttt cgcattttcg ggcgtaaacg gctgatccca agcggagtag cgagacgcgt
     9601 tcaacacggc aagcgcgctg ctcacaccca tcagcttctc gatctcaagc ggcgacaagg
     9661 tacgcaggcg cttgatcagc aactgcgact gatcgagaaa atcgggttga gtatgatcgg
     9721 caattgtcgc cggcgtggtg aaatccagtg ctttggcagg agaaagtacg aggatcatgg
     9781 cctgttacaa gaatttgatt cagcgatccg gacgaatcta ttggcgacct gcatccgccc
     9841 aacagttggg cgtttcgtag aggcgaaccc gctccagtcg caagtggtta ccatatgtgt
     9901 cttgaaatac gctgtcgagt atcgaaaacg cttgtacggc aagattctcc gaggtcggaa
     9961 tgcagtccag gatcacggtc ttgtggtccg gaatcgagtt caagaattcc acaagctgtt
    10021 tgtcaccggc gtaaaccaga aaactatgat cccaatggtc gatgaggtgc tctttcgcca
    10081 gtgatttgac ttcggagaaa tccatcacca taccgttggc gggatcgccc gcctgaacga
    10141 tgatgtcgcc actgagggta atttcgagcg catagcgatg accatgcaga tggcgacact
    10201 ggctcttgtg gtcaggaata cgatgaccgg catcgaactc aaggcggcgg gtgatgagca
    10261 ttgaaaaact cggggaaatg tagtcgatga ttatatcatt gccggattgg gcaccttgct
    10321 gtgcctggag tacgcaaatt ggcgcgagaa gccgtggagt ttgccagtca tgctgaatgt
    10381 cagaaatcat agtcgtgaca gcgcggggat gacctatgtc taccccgttc tatcgagacg
    10441 ggcgtgcggc ctgtcgctag gcatcaacct taacgtcaac aacgcctgca actgggcttg
    10501 tgtctattgc caggtgccga atctgacgcg cgggggcccg cccccagtga atcttgattt
    10561 gcttgaagat gagttacggc gctttttgaa cgggatcacg acgggtgact ctctacgccg
    10621 actcgcccct ccggaagcac agcaactggt tgatgtcgcc ttttccggga atggcgaacc
    10681 aaccagcgcg ccggagtttg ccaaggctgt tgagcgtgtc gggcgcgtgt tgcaggagtg
    10741 cgggttgctg gaaagcgtca agcttcgttt gattaccaac ggcagcttga tgcaccggcc
    10801 ggtcgtgcgt caggggatcg ccgctattgg tgcgctcaat ggagagatct ggttcaagct
    10861 cgatcgggcc actgcgattg gaatcgagcg tgtcaatggc atccatacca cgccggaaaa
    10921 ggtcaggcgg tcgctcttgc aatgcgcgga actggcgccg acatgggtgc aaacctgcta
    10981 ttttgcgatc gacggcgacg aagcaagcga ggcggaacgg tcggggtttc gcgagtttat
    11041 gaaatcggtc gcgggaaaga taaagggcgt gcacctttat ggtttggcac gcccttcaat
    11101 gcagcctgac gcaggaagac tatccaacct gtccaaggaa gacttccagc gctttgccgg
    11161 aagcatctcc gcgctaggca tcgaggttgt tgccaacctc tgacgccctg aacatcaacc
    11221 cgtcttcttc ttggccttgg cggatttctt cagtgtcttg tagagttcgg attcttcact
    11281 cttcaaatac tcgataacat ccatgtcttc gcgcttgata cgctggatat ctatctgctc
    11341 gacgtgaaca aggcggagca actcacgtac cgggcgcggc atattgcgac cgctctcgta
    11401 acgcgagcca ccgctttgcg taacgccaat ttttgaccag aactgctgtt ggttcaaacc
    11461 aagcttgcga cgaatttcgc ggggctcgga cttgtccgtg gattttgaag tgttcatagc
    11521 gatcctttcg tttggcaagc gtgccatgga gaaatcgagg catttttcgg ccccggtcga
    11581 tactactgag cgtatgactg cctatggtga tcacagtata gaacagctac atatcgattt
    11641 gcaagtagca tttttaaaga ccatgtgact acaagggttt gagtcggcaa gaaaggatcg
    11701 cgcttacgtt tcaactcgac attgggttag aatcgcgagc tttcatgacc catagttgga
    11761 ttttgcatgg cactgatagt tcagaaattc ggaggtacgt cggttgcctc gacggatcgt
    11821 atcaaaagcg tggccaggcg agtagcgcgc tggaaagccc aagggaatga cgttattgtg
    11881 gttccctcag ccatgtcagg cgaaaccaac cgcctgatcg ggctggccag ggagatttct
    11941 gccgaacccg atccacggga actcgacgtt attgcctcaa ccggtgagca agtcaccatt
    12001 gctctgctgg ccatggcaat gcacgaagaa ggtctgaagg cgaaaagcta caccggcgcg
    12061 caagtacgcg tgctgactga cagcaccttc accaaagcac gtattctgca aatcgacgac
    12121 gagaagatcc gcaaggatct ggccgaaggc aatgtcgttg ttgttgccgg gtttcagggc
    12181 gccgacgaga atggcaacat cacgacactt ggccgaggtg gttcggacac gtcggcggtt
    12241 gccctcgctg ctgcgttgaa agccgatgaa tgccaaatct ataccgacgt cgatggcgtc
    12301 tataccaccg atccgcgggt cgtgccggag gcgcgcaaac ttgacaccat cacatttgaa
    12361 gaaatgctgg aaatggccag cctgggctca aaagtcttgc agatccgctc cgtggagttc
    12421 gccggcaaat acaaggtcaa attgcgggtg ctttcaagct tcgacgacga aggcgaaggc
    12481 accctgatca ctgttgagga agaaaagaaa atggaacaac cgatcatctc cggaatcgcc
    12541 ttcaatcgtg acgaagcgaa actcaccgtg ttgggagtac ccgatcgccc cggtatcgcc
    12601 taccagatcc tcggcccgat agccgaggcc aatatcgacg ttgacatgat cattcagaac
    12661 gtcggccacg acggaacgac ggacttctcg ttcactgtca atcgcggcga ctacaccaag
    12721 gcactggggg tgctggaaac cgtcaagacc aaactggatg cgcgcggcgt cgtcggtgac
    12781 aacaaaacct gcaaggtgtc ggcggtcggt gtcggcatgc gttcgcaccc cggcgtagcc
    12841 agtcagatgt tcagtgcgct ggcatcagaa agcatcaaca tccagatgat ctcgacatct
    12901 gaaatcaaga tttccgtcgt tatcgaggaa aaatatcttg agctggccgt gcgtgtcctg
    12961 cacaaggctt tcggccttga caaggcagac taatcgcgct tttgagtttg accgggcgta
    13021 gtggaaaggc tatcatcgcg cccttccgga gacgtggccg agaggtcgaa ggcactcccc
    13081 tgctaaggga gcattcgggc aaaacctgaa tcgagggttc gaatccctcc gtctccgcca
    13141 agacagtcaa ttatgtcctt gtatataaag gatttatgta tttttacaaa ttgttgacta
    13201 tcaattcaac tatcaattga tgatacggtt ggcaagttgt cgccttccgg atggcaccta
    13261 tttcatgtaa tgctacaccg tccgttttgc tgtgaaagca gccagtcggc aaagtcgcgc
    13321 gaccttccgc taatcggcca atgctgccgt tggggtaggt cgccgccaac ggcagcaacc
    13381 gggtggtcaa cggccgtagc ccggcaagtt ggactttacg tctgcttcgc cgtatgggct
    13441 acagctggac ccgaaccgca agagacattc gctgcgtcgt tagccctgcg gcaactttct
    13501 gagcggagct gccgttcaac gtggtgactg accgcgaact cctcgtccaa agcggccagt
    13561 aagtc
//
LOCUS       DAORPM010000030        13364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_952_length_13364_cov_13.123525, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000030 DAORPM010000000
VERSION     DAORPM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 13364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13364
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_13364_cov_13.123525"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLD53_02670"
     CDS             <1..350
                     /locus_tag="PLD53_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HRC59838.1"
                     /translation="AIKLGGMARELGQHCELRSIDEARIVLCLSPIHRHLQMKPAQDK
                     LQQALCEYFGRPLQLSIELHEVSGDTPAATAQRQKHERQERAVAAVEQDDFVREVIDL
                     FDATLIESSIKPV"
     gene            371..694
                     /locus_tag="PLD53_02675"
     CDS             371..694
                     /locus_tag="PLD53_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRC59839.1"
                     /translation="MMKGGIAGLMKQAQQMQENMKKAQEQLAQVEVEGQSGAGMVKVL
                     MTCAHDVRRVSIDASVMDDKEMLEDLVTAALNDAMRRVEEVSKNSMSGFTAGLNLPAG
                     MKLPF"
     gene            694..1293
                     /gene="recR"
                     /locus_tag="PLD53_02680"
     CDS             694..1293
                     /gene="recR"
                     /locus_tag="PLD53_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRC59840.1"
                     /translation="MSTPSSLESLIKALRCLPGIGPKSAQRMAYHLLQHDRSGASSLA
                     DALQHALGALRHCQRCNTFTEADICERCLSGKRDASLLCVVETPVDMNMMEQTHAYAG
                     LYYVLIGRVSPLDGIGPRELQLDQLLARACDGVVQEVILATNFTNEGEVTAHYLAEML
                     RSRAIRVSRIARGVPVGGELEYVDAGTLAQAVRERRSLT"
     gene            1404..2000
                     /gene="petA"
                     /locus_tag="PLD53_02685"
     CDS             1404..2000
                     /gene="petA"
                     /locus_tag="PLD53_02685"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRC59841.1"
                     /translation="MSTDSKIDGDRRRLIVATAAVGGIGAITAMVPFLSSMLPSERAK
                     AAGAPVEVDIGSIGPGQMITVEWRGKPVWVLNRTEEMLSTLPKLDSAVADPKSEVDLQ
                     PESCKNETRSIKPNMWVAVGICTHLGCSPSSKFQKGPADGMAADWLGGFLCPCHGSTF
                     DFAGRVFKKMPAPTNLEVPPYTYLTDTRILIGENKKGA"
     gene            2004..3308
                     /locus_tag="PLD53_02690"
     CDS             2004..3308
                     /locus_tag="PLD53_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HRC59842.1"
                     /translation="MASNTSYEKYKSDGSLQGNLLEWVDARFPATSMWRGHLSEYYAP
                     KNFNFWYFFGSLAMMVLVIQIVTGIFLVMFYKPDASLNASGIPIAFASVEYIMRDVNW
                     GWLIRYMHSTGASAFFVVVYLHMFRGMLYGSYRKPRELIWVFGTMIFLCLMAEAFMGY
                     LLPWGQMSYWGAQVIVNLFSAIPLIGEPLSIWIRGDFVIGDATLNRFFSFHVIAVPLV
                     LLGLIAAHILALHEVGSNNPDGIEIKKKLNDKGIPLDGIPFHPYYTVKDIVGVVVFLM
                     VFAIIVFFMPEGGGYFLEYNNFFPADPLKTPPHIAPVWYFTPAYSILRSMVWPLFGLD
                     AKFWGVVAMGASVVIIAFLPWLDKSPAKSIRYRGPIYKGFLAAFVIAFLILGYLGTLP
                     PSPVGQIVSQICSAIYFGFFLLMPWYSKIDKCQPEPDRVKYK"
     gene            3305..4033
                     /locus_tag="PLD53_02695"
     CDS             3305..4033
                     /locus_tag="PLD53_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HRC59843.1"
                     /translation="MKANNMKRFLLALLFVPVTVFASGAALHLDRAPNVQDDPAALQN
                     GAKLFVNYCLNCHGLSFVRYNRLTELGLTDQQVRDNLMFTADKIGEPMRIAARPEEQK
                     KWFGVMPPDLSLVARARASGDGSGADWLYTYLRSFYLDDKRQTGWNNVIFPNVGMPHV
                     LWELQGLQALNHETHKLELVVPGQLSPDAYDKQVADLVGFLVWAGEPGAGFRRKLGYG
                     VLAFLIVLFGVSYSLKKAYWKDVK"
     gene            4134..4730
                     /locus_tag="PLD53_02700"
     CDS             4134..4730
                     /locus_tag="PLD53_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRC59844.1"
                     /translation="MMNLYSGTTCPFSQRCRIVLYEKGMDFQVIDVDLFAKQEDIAMI
                     NPYGRVPVLVERDLILYEPNIINEYIDERFPHPQLMPADPIMRARARQLLITMEREVF
                     LHIEALEKNHKTADKARQIIRDRLTEMAPVFVKQKHMLGEDFSMLDVAIAPLLWRLDH
                     YAIELPKSAAPLMKYAERIFSRQGFIDALTPSEKAMRR"
     gene            4816..5241
                     /locus_tag="PLD53_02705"
     CDS             4816..5241
                     /locus_tag="PLD53_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HRC59845.1"
                     /translation="MELPSTKPYLIRAIHEWCCDNNFTPYISVIAGKQARIPREFVRD
                     GKIVLNIGYEATGHLNIANDGITFQARFGGVARDIAVPMGNVAAVYAKENGTGMAFEP
                     SSEDLSDEGKVPVPDVTQVSEPEPPAAPPTGRPSLTRIK"
     gene            5384..8107
                     /locus_tag="PLD53_02710"
     CDS             5384..8107
                     /locus_tag="PLD53_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRC59846.1"
                     /translation="MAKEIKSTCCYCGVGCGLLIAVENNRILGVKGDPEHPANFGRLC
                     TKGAALHLTARSDYRLLHPALRLDRNAPRQRVGWNEALDHASTRFSEIIRRFGPDSVA
                     FYISGQLLTEDYYVFNKLAKGLIGTNNVDTNSRLCMSSAVAGYKQTLGADAPPCSYED
                     IALTDCLLIAGANPAVAHPIVFRRIEDARVANPQLKIIVIDPRRSETARIADLHLALR
                     PGTDIALFNGLLHVLLTEELVDRDYIAAHTEGFEALEQTVMAYTPASASGICGVPESD
                     IVRAARWFGASRASLSLYCQGLNQSSHGTHNNAALIHLHLATGHIGKPGAGPFSLTGQ
                     PNAMGGREVGGLANLLSAHRDMANPAHRDEVAGLWGIPSVPATPGKAAVDLFAALKSG
                     EIKAVWIACTNPAQSMPDQGAVRESLQAAEFVVLQEAYGNTDTAAYADLMLPATSWGE
                     KEGTVTNSERRITHVSPAVGGPGEARHDWEIVVEFARRLGQKLDNSLTEHLFPYECIE
                     DIFNEHRETTRGRDLDITGLSYAILDTAGPQQWPYPEAASEGRQRLYEDGVFATSNGR
                     ARFVPVKHQATAEKASDAYPLSLISGRLRDQWHGMSRTGTVAHLFNLDEAPEISLHPT
                     DMHARGLEAGDLVRVSNPRGDVVIRVMPGETLLPGCAWMPMHWGSQFMNSAGVNALTI
                     SATDPYSQQPELKHTTVAVEKVDLPWQLVILRKAGEGRLAASQLLEKARSLLPQFASA
                     SVGLYGRAEPLVVFRAEMSEALPEDRLREIDGLFGLDDAESAIVYADRQRQISKRAAM
                     LDGKLIGVRLAGETLAQSWLKDAMAENTLDAALIRCALAPIGKPPSQLLGKSRVVCNC
                     ADITEAQLRAELNRSTTLLQLGEKLKCGTYCGSCVPELRQMIAGQIREAAV"
     gene            8127..9110
                     /gene="ybiB"
                     /locus_tag="PLD53_02715"
     CDS             8127..9110
                     /gene="ybiB"
                     /locus_tag="PLD53_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HRC59847.1"
                     /translation="MNYAHFIREIGRGSEGARDMSFEESQQLYGAMLDGGVPDLELGA
                     IAIALRVKGESVSEMLGFLAAANERLYTLSRPQGRIRPVVIPTYNGARKGVNLTPLLA
                     LMLRRFGIPVVLHGQIEGYGRVTTAQILREFGLLPSTSQLQAQQSLDEDGLVYVPLAV
                     ISPGLNNQLALRSRLGLRNSAHSLVKMLDPFKGDGLLLAAATHPDYLNTMREMLGATG
                     AHALLLRGTEGEPVANPKRRPRIEHLYDGARDILFEAEHDSLKTLPHLPESCDAKTTA
                     DWMRRVLAGECALPLPLVNQLACCLYASGQSDDFNQAKAIVALEGSGLSLA"
     gene            9235..9990
                     /locus_tag="PLD53_02720"
     CDS             9235..9990
                     /locus_tag="PLD53_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRC59848.1"
                     /translation="MLSLLSDPEAWIAFFTLTALELVLGIDNIVFISILVDKLPPQSR
                     EKARKTGLFFAMFMRIALLLLLAWIVGATEPLFSVLGQPVSPRDIILIAGGLFLLWKS
                     TQEIHQLLEGEEGEAAKSVKATFTAIILQIIVIDLVFSLDSIITAVGMANDVSVMIAA
                     VVASVGLMMLFAKSIGTFVSDHPTIKMLALSFLLLVGMTLVADGLGHHVPKAYIYFAM
                     AFSLGVEMLNLRLRKKQSKPVHLHDPYGQKMPD"
     gene            10024..11343
                     /locus_tag="PLD53_02725"
     CDS             10024..11343
                     /locus_tag="PLD53_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRC59849.1"
                     /translation="MDLVLLAFLILLNGVLAMSEIAVITSRRARLQKLADDGSPGALS
                     ALALSHEPSGFLSTVQVGITTVGILNGAIGESALADPLTVWLSGFTLLEPYAKGVART
                     LVVVGLTYFSVVVGELVPKRLGLLAPESIATLIAKPMNVLARMTRPLVWLLSSSSTVI
                     LRVIGARRSEDPPVTNDEINVLMGQGALAGIFHESEQAIVSNVLRLDEQRISAIMTHR
                     NDIFILDLDEPEDVIRARLSENPYTRIVVCRDGLEHIVGILRTAELLKSALSGEPLDV
                     ESHLRPPLYVPEGTTTTHLLESFRKARQQCALIVDEYGELQGFVTLTDVLTSIVGELP
                     SSDIQEEQDIVERADGSYLVDGSVVIERLKSVINIEEGLPGEDENAFNTLGGLVMYVL
                     GRIPMVTDHFEVAGCCFEVVDMDKNRVDKVLLTKLQAPVEAADDAAA"
     gene            complement(11436..>12081)
                     /locus_tag="PLD53_02730"
     CDS             complement(11436..>12081)
                     /locus_tag="PLD53_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRC59850.1"
                     /translation="EVDEIIQCFEADWNRQPFDPGENSHLVWCNINGRNRVAHFIDSA
                     QETLFLENERYQDEVIIEHLVRATRRGVKIRVLARPAHTLKKGKLVEGIEGLRILEDL
                     GAKVHKLKGLKLHAKMMLADGKRCIIGSINFAPGSFDTRRELAITVHDEPVVKRVEKI
                     AEQDWTHSHALDLSDEGLLAEFEKDQAEVAEMLAIDTGKVDHGHHHEHKHKHKA"
     assembly_gap    12082..12181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12182..12604)
                     /locus_tag="PLD53_02735"
     CDS             complement(<12182..12604)
                     /locus_tag="PLD53_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRC59851.1"
                     /translation="MPDDTVQPFVDAIAAAKQSLRIKIFLFSDPTLLAAVIDAQQRGV
                     KVRVMLNPARRSGEVENEEPRAALLAAGVDVIDSNPEFDITHEKSLVIDDETAFVQSL
                     NWETKNLTVTRDYAVVTSHRHEVDEIIQCFEADWNRQPF"
     gene            complement(12784..>13364)
                     /locus_tag="PLD53_02740"
     CDS             complement(12784..>13364)
                     /locus_tag="PLD53_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HRC59852.1"
                     /translation="RLFKENGRSHFMTRRFDRIVGSAAGEKLHMQSLCALAHYDFNMA
                     GAYSYEQALLVMRQLQLPMQAIEQLFRRMVFNIVARNQDDHVKNIAFLMNKSGEWRLS
                     PAFDMTYSFNPAGAWTARHQMTMNGKRDDFTMEDFNACAKTASMKRGRAAKIVAEVQA
                     TVSQWRSFAEEAGVPDVLREKIQRTLNLKPWP"
BASE COUNT         2665 a         3695 c         3861 g         3043 t
ORIGIN      
        1 tcgcaatcaa acttggtggt atggcacgtg aacttgggca gcattgcgaa ttacgcagca
       61 ttgatgaggc ccggatcgtt ctttgcctct cgccgataca tcgccatttg caaatgaagc
      121 cagcgcagga caagcttcag caggcacttt gcgaatattt cggccgcccc ttgcagttgt
      181 cgatcgaact gcatgaggtg tccggcgata cgccggctgc gaccgcccag cgccagaagc
      241 atgagcggca ggagcgcgcc gtggcggcag tcgaacagga cgatttcgta cgcgaagtca
      301 ttgatttatt tgacgcaaca ctgattgaat cttccattaa acccgtttaa gaccagccga
      361 ggtaagcatg atgatgaaag gtggaattgc cggcctgatg aaacaggctc agcaaatgca
      421 ggaaaacatg aagaaggcgc aggaacagct tgcgcaagta gaagtcgagg ggcaatccgg
      481 tgcgggtatg gtcaaggtgc tgatgacctg tgcccacgac gttcgccgcg tgtcgatcga
      541 tgcgtctgtt atggacgaca aggaaatgct tgaagatctg gtgactgctg ccctgaacga
      601 tgccatgcgg cgtgttgaag aggtttccaa gaacagcatg tccggtttca ccgccggact
      661 caacttgccc gccgggatga agctgccttt ctgatgtcga cgccgtcgag ccttgaatcc
      721 ttgatcaagg cgttgcgctg cctgccggga atcggcccga aatcggcgca gcggatggcc
      781 tatcacctgc tgcagcacga ccggtcaggc gcaagtagtc ttgcagatgc cttgcagcac
      841 gcacttggcg cgctgcgcca ttgccagcgt tgcaatacct ttaccgaagc cgatatctgc
      901 gagcgctgcc tgtccggcaa gcgcgatgcc agcttgctgt gtgttgtcga aacaccggtc
      961 gacatgaaca tgatggagca gacgcatgct tacgccgggc tgtattacgt cctgatcggt
     1021 cgtgtctcgc cgcttgatgg catcggtccg cgtgagttac agctcgatca actgctggca
     1081 cgtgcctgcg atggcgtggt gcaggaagtc attctggcca cgaatttcac caatgaaggt
     1141 gaagtaacgg ctcattatct ggcggaaatg ctcagaagcc gcgcaattcg cgtctctcgc
     1201 attgcgcgcg gcgttcccgt tggcggtgag ctggaatacg tcgatgccgg aaccctggct
     1261 caggccgttc gcgagcgcag gtcgcttaca tgataggtat tcagcatgca cctcttcatt
     1321 accgggcaag ttcggcgtat aatccacagc tttagtttgc gctccgtttt ccaagctgga
     1381 gtttcgttct aagggtcagc acgatgagta cagatagcaa aattgatggc gacaggcggc
     1441 gcttgatcgt cgctaccgct gccgtcgggg ggattggggc gataaccgcc atggtgccct
     1501 tcctttccag catgctcccg tccgaacgcg ccaaggcggc gggtgctccg gttgaagtgg
     1561 atatcggcag catcgggccg ggccagatga ttactgtcga atggcgcggc aagccggtat
     1621 gggtcctgaa ccgcaccgag gagatgttga gcacattgcc caagcttgac agtgccgttg
     1681 ccgacccgaa atccgaggtc gatctgcagc cggaatcctg caagaacgaa acgcgttcga
     1741 tcaagccgaa tatgtgggtt gcggttggca tttgcaccca ccttggttgc tctccctctt
     1801 caaaattcca gaaaggccct gccgatggca tggcagccga ttggctgggt ggttttctgt
     1861 gcccctgtca tggttcgacc ttcgactttg ccggccgtgt tttcaagaaa atgccggctc
     1921 ccaccaacct tgaagtgcct ccgtacacct atctcaccga tacgcgtatt ctgatcggcg
     1981 aaaacaagaa gggggcgtaa gtcatggctt caaacaccag ttacgaaaag tacaagtccg
     2041 acggttcgct gcaggggaac ctgctcgaat gggtggacgc ccgcttcccc gcgacatcca
     2101 tgtggcgcgg ccatctgtcc gaatactacg cacccaagaa cttcaatttc tggtacttct
     2161 tcggttcgct ggcgatgatg gtgctggtga ttcagatcgt taccggaatt ttcctggtga
     2221 tgttctacaa accggacgcc tcactcaacg ccagcggtat tccaatcgct tttgccagtg
     2281 tcgaatacat catgcgcgat gtgaattggg gctggctgat ccgctacatg cactcaaccg
     2341 gcgcttcggc cttctttgtc gtcgtttatc tgcacatgtt tcgcggcatg ctctatggat
     2401 cctatcgcaa gccgcgtgag ctgatctggg tgttcggcac gatgatcttc ctgtgtctca
     2461 tggctgaggc tttcatgggc tacctcctgc cctggggcca gatgtcgtac tggggtgctc
     2521 aggtgatcgt gaacctgttt tccgccatcc cgctgattgg ggagccgcta tccatttgga
     2581 ttcgcggcga cttcgtgatc ggcgatgcga ccctgaaccg cttcttctcc ttccacgtca
     2641 ttgccgtgcc cctcgtcttg ctcggactca tcgccgcaca catactggcg ctgcacgaag
     2701 tcggttccaa taatccggac gggatcgaga tcaagaagaa actgaacgac aaaggcattc
     2761 cgcttgacgg catcccgttc cacccgtact acacggtaaa ggacatcgtt ggcgttgtcg
     2821 tattcctgat ggttttcgcg atcatcgtct tcttcatgcc ggaaggcggc ggctatttcc
     2881 ttgagtacaa caatttcttc ccggctgacc cgctgaaaac tccgccgcac attgcaccgg
     2941 tgtggtactt cacgccggcc tattcaatcc tgcgctcaat ggtatggcca ctgttcggac
     3001 tggacgcgaa gttctgggga gtcgtcgcga tgggtgcgtc ggtcgtgata attgccttcc
     3061 tgccctggct cgacaagagc ccggcaaagt cgatacgcta ccgtggtccg atctacaagg
     3121 gtttcctggc ggcctttgtc atcgccttcc tgattcttgg ctatctcggc acactgccgc
     3181 catcgcctgt tgggcagatc gtttcgcaga tctgcagcgc catatatttc ggcttttttc
     3241 tgctcatgcc ctggtattcg aagatcgaca agtgccaacc cgaacctgat agggtgaaat
     3301 acaaatgaaa gcgaacaaca tgaagagatt cctgctggcg ctcctgtttg taccggttac
     3361 ggtatttgct tctggtgcgg cactgcatct cgaccgggcg cccaatgttc aggatgatcc
     3421 ggcggccttg caaaacggtg ccaagttgtt cgtcaactac tgtctgaact gccacggtct
     3481 gagttttgtc cgatacaacc gtttgacgga actgggattg accgatcagc aggtgcgcga
     3541 caatctgatg tttaccgccg acaagatcgg cgagccgatg cgtattgccg cacgcccgga
     3601 agagcaaaag aagtggtttg gcgtgatgcc gccggacctg tcgctcgttg cccgggcacg
     3661 cgcgtcgggt gatggaagcg gcgctgactg gctctatact tatctgcgtt ccttctatct
     3721 cgatgacaag cgccagaccg gctggaacaa cgtgatcttt cccaatgttg gcatgcctca
     3781 tgtcctgtgg gaattgcagg gcctgcaagc gctgaatcac gaaacgcaca aactggaact
     3841 ggtcgtcccg gggcagttgt cgccggacgc gtacgataaa caggttgccg atctggtcgg
     3901 ctttctcgtc tgggccggag aaccgggtgc cggttttcgc aggaagcttg gctatggtgt
     3961 tctggccttt ctgattgttc ttttcggtgt atcctattcg ttgaagaaag cttactggaa
     4021 agacgtcaag tagaattggg cagccgtcta ttcaggtttc ggcatcgtcg gcgtaagggt
     4081 gtgatagcgc ccattgcagc cacgatgccg gttcgttttt tgaggtatga atcatgatga
     4141 atctctattc gggcacgact tgcccgttca gccagcgttg ccgcatagtc ctgtacgaaa
     4201 aaggcatgga tttccaggtg attgatgtcg atctcttcgc caaacaggaa gatatcgcca
     4261 tgatcaaccc ctatgggcgt gtgccggtac tcgttgaacg cgatttgatc ctgtacgagc
     4321 cgaacatcat caatgagtat attgatgaac gttttccaca tcctcagctg atgccggccg
     4381 acccgatcat gcgcgcgcgc gcgcggcagc tgctgattac gatggaacgc gaggtgttcc
     4441 tgcatatcga agcgctggag aagaaccaca agacggccga caaggcacgt caaatcatcc
     4501 gcgaccgcct gaccgagatg gcgccggtct tcgtcaagca gaaacacatg cttggtgaag
     4561 acttttcgat gctggatgtg gcgattgcgc ctctcctctg gcgtcttgat cactatgcga
     4621 tcgaattgcc gaaatcggct gcacctttga tgaaatacgc tgagcggata ttcagccgtc
     4681 agggattcat cgacgcactg acgccgtccg aaaaggccat gcgccgctga tccggacctt
     4741 agttttccct ggaaattcgg ttcaggcgtg tgacctagtt cgtccctgtt caatcgactt
     4801 gattcgggaa tagacatgga actgccatcg acaaagcctt atctgattcg cgccattcat
     4861 gaatggtgct gcgacaataa tttcacacct tacatttccg tcattgccgg caagcaggca
     4921 cgcatcccgc gcgagttcgt gcgcgacggc aagattgttc tgaacattgg ttacgaagcg
     4981 acaggacatc tgaacatcgc caatgacgga attaccttcc aggcgcgctt cggcggcgtg
     5041 gcacgtgaca ttgcggtccc tatgggcaat gtggccgccg tttatgccaa ggaaaatggg
     5101 accggcatgg cgttcgaacc gagtagcgag gacttgtccg atgaaggcaa ggtcccggtg
     5161 cccgacgtta cgcaggtcag cgagccggaa ccgcccgcag cgccaccgac ggggcgtccc
     5221 agtttgacgc gtatcaaata ggcacgccca tatcgaggcg tcacccgtct gagccgcacc
     5281 agaatgaagc atggttccgg gttttgtacg gccgatatca tgcaagttgt tgattttctt
     5341 gcttggcata gctgttgctc catgacgcgg aggagtggta gccatggcaa aagaaatcaa
     5401 atcgacctgt tgttactgcg gcgtgggctg cggtctgctg atcgcggttg aaaacaacag
     5461 aatccttggt gtaaaaggcg accccgaaca tccggccaat ttcggccgtt tgtgcaccaa
     5521 gggtgcagcc ctgcacctga cggcccgatc ggactaccgt cttcttcatc cggcactgcg
     5581 ccttgatcgg aatgcgcctc gacaacgagt aggttggaat gaggctcttg atcatgcctc
     5641 cactcgtttt tccgagatca ttcgtcgttt cggtccggac agtgttgcct tctatatttc
     5701 cggacagttg ctgaccgagg actattacgt cttcaacaag ttggccaaag gtttgatcgg
     5761 caccaacaat gtcgatacca actcgcgact gtgcatgtct tccgccgttg ccggctacaa
     5821 acagacgctg ggtgccgacg cgccgccttg cagctacgag gacattgctc tgacggactg
     5881 cttgctaatc gctggcgcga acccggccgt ggcgcatccg attgtcttcc gccgtatcga
     5941 ggatgccagg gtcgccaacc cccagctcaa gatcatcgtc attgacccac ggcgtagcga
     6001 aacagccagg attgccgatc tgcatctggc actgaggccc ggcaccgaca ttgccctctt
     6061 caacggcctg ttgcatgtgt tgctgacaga agaactggtc gatcgtgact atatcgctgc
     6121 gcacaccgaa ggcttcgagg cgctggagca aacggtgatg gcttacacgc cggcaagcgc
     6181 ttccgggatc tgcggcgtgc cagaaagtga cattgtccgg gccgcgcgct ggttcggagc
     6241 gtcccgtgct tctctctcgc tctactgtca ggggctcaac cagtcgtcgc atggcacgca
     6301 caataatgca gcgcttattc acctgcatct agctaccggc catatcggca agcccggcgc
     6361 cggtccgttc tcattgaccg gtcagccgaa tgcgatgggc ggtcgcgaag tgggtggctt
     6421 ggccaacctg ctttcggcgc accgggacat ggccaatccc gcgcatcgtg atgaagttgc
     6481 cgggctttgg ggtattccgt ctgtaccggc aacgccgggc aaggcggccg tcgatctctt
     6541 cgcggccttg aagagtggcg aaatcaaagc cgtctggatt gcgtgcacca accccgctca
     6601 gtccatgccg gatcagggtg cggtgcgaga atcactacag gcggcagaat tcgtcgtatt
     6661 gcaggaggct tacggcaata ccgatacggc tgcctacgcc gatctcatgt tacccgccac
     6721 ctcctggggg gagaaagagg gcacggttac caattcagag cgacgaatca cgcacgtcag
     6781 tcccgcggtc ggcggtccgg gcgaggcccg tcacgactgg gagatcgttg tcgagttcgc
     6841 acgccgcctc gggcaaaaac tggacaactc gctgaccgaa catcttttcc cgtacgaatg
     6901 cattgaagac atctttaacg aacatcgtga aaccacacgt gggcgcgacc ttgatatcac
     6961 cggcctgagt tatgccattc tcgataccgc aggaccgcaa caatggcctt atcccgaagc
     7021 agcgagcgag ggcaggcagc gcctctatga agatggtgtc tttgcgacga gcaatggccg
     7081 agcgcgcttt gtccccgtta aacatcaggc tactgccgag aaagccagtg acgcgtatcc
     7141 cttgagtctg atctcggggc gtctgcgcga tcagtggcac ggaatgagcc gcaccgggac
     7201 ggtcgcacat ttgttcaacc tcgatgaagc gcccgagatt tcattgcatc ccaccgatat
     7261 gcatgcgcgc ggtctcgaag ccggtgatct ggtacgcgtc agcaacccac gcggcgatgt
     7321 cgttatacgc gtcatgccgg gagagacatt gctgccgggt tgcgcatgga tgcccatgca
     7381 ctgggggagc cagttcatga acagtgccgg tgttaacgcc ttgaccattt cggcaaccga
     7441 cccgtattcg caacagcctg agctcaagca cacgacggtg gctgtcgaaa aggtcgattt
     7501 gccgtggcaa ctggtgattc tgcgcaaggc gggagagggg cgactcgctg cgtcgcagct
     7561 actggagaaa gcacgctcgc tattgccgca gtttgccagt gcttcagtgg ggctctatgg
     7621 gcgcgccgaa ccgctggttg tctttcgtgc ggaaatgagt gaagctctgc cggaggatcg
     7681 cttgcgcgag atcgacggat tgttcggcct tgatgacgcg gaatcggcca ttgtttatgc
     7741 cgaccgccaa cgccagatca gcaagcgagc cgctatgttg gatggtaagc tgatcggcgt
     7801 acgacttgca ggtgaaacgc ttgcgcagtc atggctcaag gatgcgatgg cggaaaacac
     7861 gcttgatgcg gcattgattc gctgtgctct tgctccgatc ggcaaaccac cgagccagtt
     7921 gttgggtaaa agtcgcgttg tctgcaattg tgccgatatt accgaggccc agctcagggc
     7981 cgagttgaat cggagtacaa cgcttttgca acttggagaa aaattgaaat gcggaactta
     8041 ctgcggctcg tgcgtgcccg aactcaggca gatgatcgct ggccagattc gggaggctgc
     8101 tgtttgaaag cggatacggg agtggcatga attacgcaca tttcattcgg gagattggtc
     8161 gtggcagtga aggtgcgcgc gacatgtcgt ttgaagagtc gcaacaactc tacggcgcca
     8221 tgcttgacgg cggcgtgcct gaccttgagc ttggtgccat agccatcgcc ttgcgcgtga
     8281 aaggcgaatc cgtaagcgag atgcttggtt tcctggccgc tgccaatgaa cggctataca
     8341 ccctttcccg cccacaggga cgtatccgtc cagtcgtcat ccccacttac aacggagcac
     8401 gcaaaggcgt aaatctcacg ccattgctgg cattgatgtt gcggcgtttc ggcattccag
     8461 tggtgttgca cggtcagatc gaaggctacg gtcgtgtgac aacggcgcag attcttcgag
     8521 aattcggtct gctgcccagt accagccaat tgcaggcgca gcagtcgctg gatgaagacg
     8581 gtctggttta tgtgccgctc gccgtaattt cgcccggttt gaacaatcag cttgctctgc
     8641 gcagccgtct tgggttgcgc aacagcgcac atagtctggt caagatgctc gatccgttca
     8701 agggggacgg cttgctgctt gccgcagcca cccaccccga ttacctcaac acaatgcggg
     8761 aaatgctcgg tgcgacaggc gcacacgccc tgttgttgcg tggcacggag ggtgagcctg
     8821 ttgccaaccc caagcgacgt ccacgtatcg aacatttata cgatggcgca agagacatcc
     8881 tcttcgaggc cgagcacgac agtctcaaga cgttgccgca cttgcccgaa agctgtgacg
     8941 ctaaaacgac tgcagattgg atgcggcgtg ttctcgcggg tgaatgtgct ctgcctctcc
     9001 ctctcgtcaa tcaacttgcc tgctgcctgt atgccagcgg tcagtccgat gacttcaacc
     9061 aggccaaagc catcgtcgcg cttgagggat ccggcctgag cctggcttga tcgggggcat
     9121 cgccatgatc gctggcgatt gggttggtga aaacggggtg ctggtataat ccgccgcata
     9181 ggggggctgt tctgccaccc cgtcgcggtt aatttgtcgg tctaatgaat ggaaatgctg
     9241 tcactgttgt ctgatccgga agcgtggatt gctttcttca cgctaacggc gctggagctg
     9301 gtactgggga tcgacaacat cgtcttcatc tccatcctcg tcgacaagtt gccgccgcaa
     9361 agtcgggaga aggcgcgcaa gaccggcttg ttctttgcga tgttcatgcg tatcgccctc
     9421 ttgcttctcc tggcctggat cgtcggcgca accgagcccc tgttcagcgt actgggccag
     9481 ccggtatcgc cgcgcgatat catcctgatt gcgggggggc tctttctgct ctggaagagc
     9541 actcaggaaa tccatcagtt gcttgagggc gaagagggcg aggcagcaaa gtcggtcaag
     9601 gcgacattca cggccatcat tctgcagatc attgtcatag accttgtgtt ttcgctggat
     9661 tccatcatta ccgctgtcgg catggcgaac gatgtttcag tgatgatcgc ggctgtcgta
     9721 gcgtcggtcg gattgatgat gctctttgcc aaaagcatcg ggacgttcgt ttcggatcat
     9781 ccgacgatca agatgctggc cctgtcgttt cttctgttgg tgggcatgac actggtcgcc
     9841 gatggtttgg gtcatcatgt gccgaaggcc tatatctact tcgccatggc gttctcgctg
     9901 ggagtggaaa tgctcaatct gcgcttacgg aagaagcagt ccaaacccgt ccacttgcat
     9961 gacccgtacg gtcagaagat gccggattga atgatggatc gttccggttt tatgaacgca
    10021 taaatggact tagtactact cgctttcctt attcttctca acggcgttct cgcgatgtca
    10081 gagatcgccg ttattacctc gcgcagggcc cggttgcaga agcttgcgga cgacgggagt
    10141 cccggtgcgt tgtcggcgct ggcactgagc cacgagccgt ccgggtttct ttccactgtt
    10201 caggtcggta tcactacagt aggcattctg aatggcgcca ttggtgagag tgcgctggct
    10261 gacccgctga ccgtttggct gtcgggattt accttgcttg agccgtatgc aaagggcgtg
    10321 gcccgtaccc tggttgttgt tggcttgacc tatttctcgg ttgtcgtcgg agagttggtt
    10381 cccaagcgtc tgggtttgct ggcaccggag tcgatcgcca cgttgatcgc caagccgatg
    10441 aacgtattgg cgcgcatgac ccgcccactg gtctggctgc tctcttcgtc atccactgtg
    10501 attctgcgtg tgatcggtgc gcgccgcagt gaagacccgc cggtaaccaa cgacgaaatc
    10561 aatgttctga tggggcaggg tgccttggca ggcatttttc atgaaagcga gcaggccatt
    10621 gtttccaatg tgcttcgcct cgacgaacag cgcatcagcg cgatcatgac gcaccgcaac
    10681 gacatattca tacttgatct tgacgagccg gaagatgtta ttcgcgcgcg actatccgaa
    10741 aacccctaca cgcgtatcgt cgtctgtcgg gacggactgg agcacattgt gggcatcttg
    10801 cgtactgccg aactcctcaa gtccgcactg tccggtgagc cgcttgatgt cgagagccat
    10861 ctgcgtccgc cgctctatgt gcccgaagga acgacaacga cgcatctgtt ggaaagcttt
    10921 cgcaaggcgc gccagcaatg tgcgctgatc gtcgatgagt atggcgagtt gcaagggttc
    10981 gtcactctca ccgatgtgct gacctcgatt gttggcgaac ttccctcgtc ggacattcag
    11041 gaagagcagg acatcgtcga gcgcgccgac gggtcgtatc tggtcgatgg aagcgtggtg
    11101 atcgaacgcc tcaaatcggt gataaacatc gaagaaggtc tgccgggcga agacgaaaat
    11161 gcgttcaata ccctgggcgg tctggtgatg tacgttctcg gtcgaattcc catggtgacc
    11221 gatcattttg aggtggcggg ttgctgcttc gaagtggtcg atatggacaa gaaccgcgtc
    11281 gacaaagtgt tgctgaccaa actgcaagcg ccggtcgagg ctgcagacga cgctgccgcc
    11341 tgaccggcag ggcacaccgg acttcctgcc gaccaactgg cagggccacc gagggcagat
    11401 cagaaaggga gaccggcgga aacctgttct ccacactaag ctttgtgctt gtgtttgtgc
    11461 tcgtggtgat gcccatgatc taccttgccg gtatcaatgg ccagcatctc ggcgacctct
    11521 gcctggtctt tctcgaattc ggcgagcaga ccttcgtcgg aaagatccag ggcgtgggaa
    11581 tgcgtccagt cttgttcagc aattttctcg acgcgcttga cgaccggctc atcgtggact
    11641 gtgatggcca gttcgcgccg agtgtcaaaa ctgccggggg caaaattgat cgaaccgatt
    11701 atgcagcgct tgccgtcggc cagcatcatc ttggcgtgaa gttttagccc cttcaacttg
    11761 tgcaccttgg cgccgaggtc ttccaggatg cgcaggccct cgatgccctc gaccagcttc
    11821 cccttcttga gtgtatgggc ggggcgtgcc agcaccctga tcttcacacc gcgtcgcgtg
    11881 gcgcgcacca gatgctcgat gatgacttcg tcctggtagc gctcgttttc gaggaacagg
    11941 gtttcctgcg ccgagtcgat gaagtgcgcc acccggttgc gcccgttgat gttgcaccag
    12001 accaggtgcg agttctcccc tggatcgaag ggctggcgat tccagtcggc ttcgaagcac
    12061 tggatgatct cgtcgacctc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 ngaagggctg gcgattccag tcggcttcga agcactggat gatctcgtcg acctcgtgcc
    12241 ggtgcgaggt caccacggcg taatcgcgag tcaccgtcag atttttggtt tcccagttca
    12301 gcgactggac gaaagctgtt tcgtcgtcga tcaccaaaga cttctcgtgc gtgatatcga
    12361 attccggatt gctgtcgatc acatcgacgc cggcggccag tagcgccgca cggggctcct
    12421 cgttttcgac ctcgccgctg cgccgcgccg ggttgagcat cacccgcacc ttgacgccgc
    12481 gttgctgcgc gtcgatcacc gccgccagca aggtcgggtc cgagaacagg aagatcttga
    12541 tgcgcagcga ctgtttcgcc gcagcaatgg catcgacgaa gggctggacg gtgtcatccg
    12601 gcaaaactat gagagagcga tgcatgagag gtggactccg agggttgtct gctggcaggt
    12661 cgcgaacagt tgtggcgctg aactccggga aaagcacgag agcgtgtttg actgacaggg
    12721 aaatatatcg ctggggaagt tccggtgtct accaatatcg ccaaggcagg tactcgcccg
    12781 gattcatggc cagggtttca gattcaacgt gcgctgaatt ttctcgcgca agacatcagg
    12841 cacgcctgcc tcttcagcaa acgatctcca ttgcgatacc gtcgcctgca cctcggcaac
    12901 gattttcgca gcgcgcccgc gtttcatcga agccgtcttt gcgcaggcgt tgaagtcctc
    12961 catcgtgaaa tcgtcgcgtt ttccattcat cgtcatctga tggcgcgccg tccacgcacc
    13021 cgcaggattg aagctgtagg tcatatcaaa ggccggcgac aaccgccact caccggattt
    13081 gttcatcagg aaggcgatgt tcttcacgtg gtcgtcctga ttgcgggcca cgatgttgaa
    13141 taccatgcgc cgaaacagtt gctcgatggc ctgcatcggc aactgcaatt gccgcatcac
    13201 cagcaacgcc tgttcgtagc tgtaggcgcc tgccatgttg aaatcgtagt gcgccagcgc
    13261 gcagagggat tgcatgtgaa gtttttcacc ggcagcactg ccaacaatgc gatcaaagcg
    13321 gcgcgtcatg aaatgcgagc ggccgttctc cttgaatagc cggc
//
LOCUS       DAORPM010000031        13122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_975_length_13122_cov_11.302594, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000031 DAORPM010000000
VERSION     DAORPM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_13122_cov_11.302594"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PLD53_02745"
     CDS             complement(<1..171)
                     /locus_tag="PLD53_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRC59853.1"
                     /translation="MTHYIFIIIGAVLVNNVVLVKVLGLCPFMGVSKKLETAFGMGAA
                     TTFVLTMGTGASY"
     gene            complement(292..1350)
                     /locus_tag="PLD53_02750"
     CDS             complement(292..1350)
                     /locus_tag="PLD53_02750"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRC59854.1"
                     /translation="MIYSLIRNVFFSLDPEMAHELGMSGVDFLNRAGVSCLLANPPPS
                     NPVEAMGLKFPNPVGLAAGLDKNGAHVDALAALGFGFIEIGTVTPRPQPGNPKPRMFR
                     IVQKKAIINRLGFNNYGVDKLLSNISTAQFPKRGGILGINIGKNFDTPIENAVDDYLI
                     CLERVYSAASYVTVNISSPNTKNLRELQKDEALDALLGRLKAEQSRLAEKHGKYVPLA
                     LKIAPDLEDAQIQSIADLLRQHRMDGVIATNTTLSRDGVEGWLHADETGGLSGTPLFT
                     RSTAVLKKLAVALAGELPIIGVGGIMSGEDAVAKLEAGASLVQIYSGFIYRGPALVGE
                     AAAAIAKAGTSNRPRIEL"
     gene            1489..2538
                     /locus_tag="PLD53_02755"
     CDS             1489..2538
                     /locus_tag="PLD53_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRC59855.1"
                     /translation="MISANAIQITPEILSLIAGVDEFKGAWRALGTLAPDRLSALRRV
                     ATIESIGSSTRIEGSRLSDREVENLLSNLQIKPFSTRDEQEVAGYAEVMEFVFSSWQD
                     IALTENHIKQLHRDLLTYSEKDAWHRGSYKTSTNNVVAFDEDGTQLGVVFATATPFDT
                     PPLMTELVTWFNAERQAARLHPLLLIGIWVVVFLEIHPFQDGNGRLSRVLTTLLLLQA
                     GYAYVPYSSLESVIEQSKEAYYLALRQTQGTIRTAKPNWQPWLVYFLRALAEQVRRLN
                     RKVEREKLVLAALPELALKIVEFAREHGRITMGDAIRLTGGSRNTLKQHFRALVEQGR
                     LGQHGAGRGVWYELR"
     gene            2719..3657
                     /locus_tag="PLD53_02760"
     CDS             2719..3657
                     /locus_tag="PLD53_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59856.1"
                     /translation="MGEIISCPKCTNQAPVYSAIYFIGKLLDKYFEAQREIGRLQGRS
                     GSNADCPSQVSTEQPSLDEIDLSNTDQLASELQHGPIYDWFNRKQIKVVANVRGVDTT
                     GFFDEVAVSIGHNVPVLKEVLERIRWAQQKEYSSVTVHLEKKSEEDARAITAFCQQLY
                     DFSFVAKCFHNRKESNIRLIVQTAPAIRRFFGGEWLEWYALMSGLQYIKERQKRFSCA
                     RNLAITLQNDETYELDVFMLVDGKLPVCIECKTGEFRQNIDRYLTLRKRLGIGRGNFI
                     MCIAGLSEEHARGLTATYDLTFTNEQELPTQLARIF"
     gene            complement(3813..5042)
                     /locus_tag="PLD53_02765"
     CDS             complement(3813..5042)
                     /locus_tag="PLD53_02765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRC59857.1"
                     /translation="MNRLVTALPGFVLENRLEFLFATWRKKFGEHPIAVELWNGKRFA
                     LGEPPRLLLRINSRRALERLMRPSLASLGTAYVEGELDFEGHMRDAFHTIAGFLSSLY
                     QPNQRRKLRKTLHTRQLDSASIEHHYDVSNDFYRLWLDENMVYSCAYFRSADDSLEQA
                     QVQKINHILNKIQLRPGERLLDIGCGWGALVLQAARKYGARCVGITLSQQQFEEASRR
                     VQVAGLQDRCEIRLQDYRDVTEEFDKITSVGMFEHVGLNHLRDYFRKIYALLPDGGIA
                     LNHGITSTDPDSEESPYGGGDFIHRYVFPNGELPHIGLLLKEMSAAGLETADVENLRR
                     HYARTLEHWTDRFENNAAQLRRIAGDKRYRVWRAYLAGCAYGFANQWISLYQIVACKA
                     DKQSAYALPMTRDYMYS"
     gene            complement(5058..5399)
                     /locus_tag="PLD53_02770"
     CDS             complement(5058..5399)
                     /locus_tag="PLD53_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59858.1"
                     /translation="MNTDFDSDQRILSPARRARRSILALTTIMLLVMGSSAVKRWVQD
                     NVFLTSGPDGESSLYYHPPFSKPGTLVAVFGATPDNLTRASSIQTTQAEIKVIETTFE
                     PATRLTAYHLR"
     gene            complement(5600..5734)
                     /locus_tag="PLD53_02775"
     CDS             complement(5600..5734)
                     /locus_tag="PLD53_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59859.1"
                     /translation="MPAGKPGALENLFVIAAITHGMNKLVCYGSNIRVVVSLSDVVLI
                     "
     gene            complement(5938..6390)
                     /locus_tag="PLD53_02780"
     CDS             complement(5938..6390)
                     /locus_tag="PLD53_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRC59860.1"
                     /translation="MTDLARYNPFRGLTRFNPFGRDMDDLLTGFFPTPMSLNPMADLH
                     MPIDITEDDKVYKVRAEMPGFNKEDISVSVNGDQVTISAETKKEKEEKKNGEKIVHRE
                     CYCGRQFRSFTLPQAADETNTKARYEDGVLNLELPKKAGDNQKKITVN"
     gene            6754..8472
                     /locus_tag="PLD53_02785"
     CDS             6754..8472
                     /locus_tag="PLD53_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC59861.1"
                     /translation="MMNENDRFARQDKFRQPVSVAARSTVIEEHLPEDLTGLSSDEIG
                     EMVLELRAHQIELEMQNENLHRTQAALDASRALYYDLYNLAPVGYCTLSEAGVILEAN
                     HRIAALLGTMRGTLIKQPIFRFIHREDQDVFYFHRKRLLESSQPQQCELRMLTVGGTL
                     LWMHLNSNATRDDAGTVMLRITLNDITERKRFEDELVAARKLAEQAVLVKSRFLAAAS
                     HDLRQPLQAINLFNDALIGTGLSDEQKQISGHLSQSISSLNELLNVLLDLSKLDAGII
                     KANPEVIEVEKFFLRCDAEFSPITKRKRLSFRLCFPLKPMAIIADRELLRSLMGNLIF
                     NAIKYTQQGGVLVAIRRRGTKALIQVWDTGIGIPAEHIHSIFEEYFQIENPERVRRRG
                     LGLGLAIVRRQAKLLGTEVACRSRPGKGSVFEFSLPLAVEAQEEVPPQNAIDRAIVDA
                     YKGRRIVVVEDDAMVANAIKLSLESVGMCVAVFQNAEEALSDAETLKADFYVSDYRLP
                     GLDGERFLENILKRSTKPFRAVLLTGDTTPGWIEKARSLRWPVLFKPIDLPQLLTTIQ
                     SQAAST"
     gene            8474..9091
                     /locus_tag="PLD53_02790"
     CDS             8474..9091
                     /locus_tag="PLD53_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC59862.1"
                     /translation="MAAKDTVFVIDDDSAIRNLLARMLEGRGINVECFESGEDFLQVC
                     RWLPRSCAIVDMNLPGLDGTQIQAELARRDILLPIVFLTGHGDIPMSVRAIKAGASDF
                     LSKPVSSAALLASVEAALNESERLNSRIGDTVDAAVRLASLTEREQDVLRLAVEGLAN
                     KVIARELGISHRTVEIHKSRIMQKTGATSLLELARLYDSSIETSN"
     gene            complement(9132..10691)
                     /gene="ahpF"
                     /locus_tag="PLD53_02795"
     CDS             complement(9132..10691)
                     /gene="ahpF"
                     /locus_tag="PLD53_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HRC59863.1"
                     /translation="MLDANLKAQLKAYLERLQRPIELVATLDDSDKAQEMRSLLDDIA
                     LLSDKVGVRNDGVDTRKPSFAVARPGEPARIGFAGLPMGHEFTSLVLALLQTGGHPPK
                     VEAELIEQIRAIPGDFHFETFISLSCHNCPDVVQALNLMAVLNPGISHTMIDGALFQD
                     EVNDRRIMAVPTVFLNGEPFGQGRMTLEEIIAKIDIGAVAREAEKLAAKEAFDVLIVG
                     GGPAGAAAAIYAARKGVRTGVVAERFGGQVLDTLAIENFISVKETDGPKLSAGLEQHV
                     REYDVDIMNLQRAEALVPGSNGLTEVRLASGATLKSKSVIISTGARWREMNVPGEKEY
                     RGRGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGVVAHVTLLEFDKALRADAV
                     LQKKLFSLPNVTVITSALTTEATGNGEKVNGLIYKDRTNDEVHTIPLEGIFVQIGLLP
                     NTDWLKGTIGLSAHGEIEVDARGQTSLPGVFAAGDCTTVPFKQIIIAMGEGAKAALSA
                     FDHLIRSSAPG"
     gene            complement(10764..11330)
                     /gene="ahpC"
                     /locus_tag="PLD53_02800"
     CDS             complement(10764..11330)
                     /gene="ahpC"
                     /locus_tag="PLD53_02800"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HRC59864.1"
                     /translation="MKSLINTEILPFKATAYHNGKFGPVSDADLRGKWSVVFFYPADF
                     TFVCPTELGDLADEYADFQKMGVEVYSVSTDTHFTHKAWHDASETIKKIHYPMIGDPT
                     GAITRNFGVMIEEEGLALRGTFVINPEGVIKVCEIHDLGIGRDAKELKRKIQAAQYVA
                     THPGEVCPAKWTPGEATLAPSLDLVGKI"
     gene            11546..>13122
                     /locus_tag="PLD53_02805"
     CDS             11546..>13122
                     /locus_tag="PLD53_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HRC59865.1"
                     /translation="MQFGAKPLFENVSVKFGEGFRYGLIGANGSGKSTFMKILDGELE
                     ATAGNVSKDAHERMAYLRQDQFAFEDQRVIDVVMMGHQEMWACMQEKDAIYVNPDASE
                     EDFMHAAELESRFAEYGGYTAEARAGELLLGVGIPSEQHFGPMSEVAPGWKLRVLLTQ
                     ALFSDPDILLLDEPTNNLDIATIRWLENVLNDRNSTMIIISHDRHFLNQVCTHMADLD
                     YGKITVYPGNYDDFMEASSQARQQQQNANARAKERISELQDFVRRFSANKSKAKQATS
                     RVKLIEKLRPEDVKPSSRQYPWIRFDYDEKEKLHRQAAEVENLTFAYAGSEHKIFDDF
                     TFTLNAGDRVAVIGENGVGKSTFLKLMMGELQPQSGSIKWTEKARRGYYAQDHADDFK
                     GDTSLVEWIAGYARANAAEGEDLETLIRGTLGRLLFSGDEVRKAVKVISGGEQGRMIF
                     GKLMLMKNNVLVLDEPTNHLDMESIESLNTALEKFKGTLLFASHDREFVSSLATRVLE
                     IRLDGTVVDYLGNYEEYL"
BASE COUNT         2884 a         3653 c         3753 g         2832 t
ORIGIN      
        1 atagctggcg ccggttccca tggtgagcac aaaggtggtc gccgcgccca tgccaaaggc
       61 ggtttccagt ttcttggaca cgcccatgaa ggggcagaga ccgagaacct tcaccagtac
      121 gacgttgttg accagaacag cgccgatgat gatgaaaata tagtgggtca tgatccgtca
      181 ggcactgcgg gaaaaggcgg caagggcaac tgcgaaagcg gctgcaaggg ctgaaaagcc
      241 ttggaattat cctctattgg cagagggcgt tcaaccctgc tggctgtgga gctatagctc
      301 aattcgcggt ctgttcgagg tgcccgcctt ggcgatggcg gcagccgcct ccccgaccag
      361 ggcagggccg cgatagatga agcctgaata gatctgaacc aggctcgcac cggcttccag
      421 tttggcgacg gcatcctcgc cgctcatgat gccgccgaca ccgatgattg gcagctcgcc
      481 ggccagtgcg acggcgagtt tcttcagcac ggccgtcgag cgcgtgaaca gcggagtgcc
      541 cgacagtccc ccggtttcgt cggcgtgcaa ccagccctca accccgtcgc gcgacaaggt
      601 ggtgttggtg gcgatcacgc cgtccatgcg atgctggcgc agcaggtcgg caatcgactg
      661 aatctgcgcg tcttcaaggt ccggagcgat tttcagcgcc agcggaacat acttgccgtg
      721 cttctccgcc agtcgagact gctcggcctt gaggcgaccg agcagcgcat cgagtgcctc
      781 gtccttctgc aactcacgca gattcttggt gttcgggctg gagatattga ccgtgacata
      841 gctggccgca ctgtacaccc gctccagaca gatcaggtaa tcgtctacgg cgttttcgat
      901 gggcgtgtcg aagttcttgc cgatgttgat tccgaggatg ccgccgcgct tcgggaactg
      961 cgcggtcgaa atgttggaaa gcagcttatc gacaccgtaa ttgttgaagc ccaggcgatt
     1021 gatgatggcc ttcttctgga cgatacggaa catgcgcggc ttgggattgc ccggctgcgg
     1081 gcgcggtgtc acggtgccaa tttcaatgaa accaaagcca agagcggcca gtgcatcgac
     1141 atgtgcgccg ttcttgtcca atcctgcggc cagaccaacc ggattgggga acttcaagcc
     1201 catggcctcg accgggttgg atggcggtgg atttgccagc aggcaggaga cacccgcccg
     1261 gttgagaaaa tcgactccgg acatcccgag ctcgtgagcc atttcggggt cgagcgaaaa
     1321 gaaaacgttg cgtatcaggg aataaatcat cgacggattt tacccgaagc cgccgcttcg
     1381 caggattgga atggcggtga tcttcacagg tcgcgtccgg catgcgccgg ctgtattcga
     1441 tagatcactc tgttgaaatg ggtgtaaatg ggtgtagcat tggaacccat gatctccgcg
     1501 aatgcaattc agataacacc cgaaatcctc agtctcatcg ccggtgtcga tgagttcaag
     1561 ggggcctggc gtgcgctcgg tacgttggcg cccgaccggc tatccgcatt gcgcagggtc
     1621 gctacgatcg aaagtatcgg ttcgtctacc cgcattgaag gtagcaggct gtccgatcgc
     1681 gaggtcgaga acttgctgtc caatctccag atcaagcctt tttcaaccag agacgaacaa
     1741 gaggtggccg gttacgccga ggtcatggag tttgtgtttt catcctggca ggacatcgcg
     1801 ctgaccgaga accacatcaa gcaactgcac cgtgaccttc tgacctacag cgagaaggat
     1861 gcctggcatc ggggcagcta caagacctcc accaataatg tagtggcctt tgacgaagac
     1921 gggacgcagc tcggtgtcgt ctttgccacc gcgacaccat tcgatacgcc accattgatg
     1981 accgaactgg tcacctggtt caacgcggaa cgtcaagcgg cgcggcttca ccccttgctg
     2041 cttatcggca tctgggtcgt ggtttttctg gagattcacc ccttccagga tggcaacggc
     2101 cggcttagcc gcgtcctcac caccttgctg ttgttgcagg ccggctacgc ctatgttccc
     2161 tacagctcgc tggaaagcgt gatcgagcag agcaaggagg cgtactacct cgccctgcgg
     2221 cagacccagg gcacgattcg caccgcgaaa ccaaattggc aaccgtggct ggtctacttt
     2281 ctgcgagcgc tcgccgaaca ggtccggcgc ctcaatcgta aagtcgagcg cgaaaagctg
     2341 gtgctggctg cattgcccga gctcgccctg aaaattgtgg aatttgcgcg ggaacatggc
     2401 cgtatcacca tgggcgacgc gatccggctg accggcggca gtcgcaacac gctcaagcaa
     2461 cattttcggg cgctcgtgga gcaaggacgc cttggacagc acggcgcggg taggggagtc
     2521 tggtatgagt tgcgataggg gaagattcat ggggcagaac ctcgggaatt gcgtcctttg
     2581 tactgtttag ccttggcttt agctatgatc acggtatgct tggctcgtcg tcgaaactaa
     2641 acaacactca cgctgcttac tatggctatt tttcgctgca ataaatgtgg gtacctgcaa
     2701 gaacagccgg agggccgttt gggtgaaatc atctcctgtc ctaagtgcac aaaccaggca
     2761 cccgtctaca gcgccatcta tttcattggc aagctactgg acaaatactt cgaagcccag
     2821 cgcgaaattg gtcgcctgca agggcgttcg ggcagcaatg ccgactgccc ttcgcaggtt
     2881 tcaacggaac aaccttcgct cgatgaaatt gatctttcca acaccgatca actggcctcc
     2941 gaactgcagc acggtccaat ctacgactgg tttaaccgaa agcagatcaa agttgtggct
     3001 aatgtgcgtg gtgttgacac caccggattc ttcgatgaag tcgcggttag cattggtcac
     3061 aatgtgccgg tcctgaaaga ggttctagaa cgcatacgat gggcacagca gaaggaatac
     3121 tcaagcgtaa ccgtccacct tgaaaagaaa tcggaggaag atgcgcgtgc cataacagct
     3181 ttttgccaac agttgtatga cttttctttt gtcgccaaat gcttccacaa tcgtaaagaa
     3241 agtaacatcc gcttgattgt gcaaaccgcg ccagcgatcc gccgtttttt cggcggtgag
     3301 tggctggaat ggtatgccct gatgagcggc ctgcagtaca tcaaagaacg tcagaagcgc
     3361 ttttcctgcg cgcgtaacct tgccatcaca ctgcaaaacg acgaaaccta cgagctcgat
     3421 gtgttcatgc tggtcgatgg caagcttccc gtttgtatcg aatgcaagac gggtgaattc
     3481 cgccagaaca tcgatagata cctgacactg cgaaagcgcc ttgggatcgg tagagggaat
     3541 ttcatcatgt gtattgccgg tctcagtgaa gagcatgcca gaggtttgac cgccacgtat
     3601 gacctgacat tcaccaacga acaagaattg cccacccagc tcgcccggat tttctgatca
     3661 acccttttct tgccactctg caacaccgta gcttgcgatc cagacttgat ctgaattcgg
     3721 aatcattggt acgtcagacg aataccagtt ctccttgatg ggcagttgca attcgtccgg
     3781 ggacgaaaac acctgacata tcagtcagca attcaagagt acatatagtc gcgcgtcatc
     3841 ggcagggcgt aagcgctttg tttgtcggcc ttgcaggcga cgatctgata aagcgatatc
     3901 cactgattcg cgaatccata ggcgcacccg gccaaatacg cgcgccaaac ccggtagcgc
     3961 ttgtctccgg cgatccgacg aagttgtgcg gcattgtttt cgaaacggtc ggtccagtgt
     4021 tcgagcgttc tcgcgtaatg gcgacgcaag ttttccacat cggcggtttc cagcccggcg
     4081 gcgctcattt ctttcagcaa caggccgata tgcggcaatt cgccgtttgg aaagacgtag
     4141 cggtgaatga aatcgccacc gccgtaaggc gattcttcgg aatcagggtc ggtagaagtt
     4201 atgccgtggt tcaatgcaat tccgccatcg ggcagcagtg catagatctt tcggaaatag
     4261 tctcgaaggt ggttcaagcc gacgtgttcg aacattccga cgctggtgat tttgtcgaat
     4321 tcttcggtga catcccgata gtcttgcagg cggatctcgc agcgatcctg aagaccggca
     4381 acctgcaccc gacgtgacgc ttcctcgaat tgctgttggg acaaggtaat gcccacgcag
     4441 cgcgccccat actttcgggc tgcctgcaaa accagcgcgc cccagccaca accgatatcc
     4501 agcagacgct ctcccggacg caactgaatc ttgttgagga tatgattgat cttctggact
     4561 tgcgcttgct ccaggctgtc gtcggccgat cgaaaatatg cgcacgagta aaccatgttc
     4621 tcatccagcc acaaacgata aaagtcgttt gaaacatcgt agtggtgttc aatggatgct
     4681 gaatcgagct ggcgggtgtg tagcgtcttg cgaagctttc ggcgctgatt cggttggtag
     4741 agcgatgaaa ggaaaccggc gatggtgtga aaggcgtccc gcatatgccc ttcaaaatcc
     4801 aactcccctt cgacgtaggc tgtgcccagc gacgccagcg agggacgcat caagcgctct
     4861 agcgcccttc tcgagttgat acgcaataga agtcggggag gttctccgag agcaaagcgc
     4921 tttccgttcc atagctcaac cgcaatcggg tgttcgccga acttcttgcg ccaagtcgca
     4981 aagagaaatt ccagccgatt ctccagcaca aatcctggca gcgcggtaac aagtcgattc
     5041 attgttactc ctcccgatca gcgcaggtga tatgccgtta gcctggtggc aggctcgaag
     5101 gtggtttcaa ttaccttaat ttctgcctgg gtcgtctgta tggaggatgc ccgagtcaaa
     5161 ttgtcgggcg ttgcaccaaa gacggcaacc agggttccag gcttgctgaa cggcggatgg
     5221 taatagagac tagattcgcc atccggaccg ctcgtgagga atacattgtc ttgcacccag
     5281 cgctttaccg cgcttgaacc catcaccaga agcatgatgg tggtcaaggc caggatgctc
     5341 cgccgcgcac gtcgggcggg ggagagtatg cgttgatcag agtcaaaatc agtgttcatg
     5401 attgtgctcc tgaagtgagt gtatttcgct taggggaccg gttttgccgt tgatcgtcga
     5461 tgtgccaagt ttcagagact tcggggcaag cctggagctc aaggcgccga gcgcgaagcc
     5521 gacgcaaacg gacacgatca gccggatcgt gattgacaag acgcgatgca gacgctcagt
     5581 gctcccgaaa ttcatccatc tagatcaaaa ccacatcgga cagggaaacg acaaccctga
     5641 tgttcgagcc atagcagacc agcttgttca ttccgtgtgt gatggccgca atcacgaaca
     5701 ggttttccag cgcaccaggc ttcccggctg gcattacaag cttctcgaac gttcgatact
     5761 tcaaccgaac ataccgcccg actagcgctt tcagcttttt tctcatgaca atcgacaccg
     5821 atgcgaagct tgagccagtg ccttgcgcgc gcgctgacga cacggtccga agtgatggca
     5881 ggtagcgcct ggctatgtcc ttgaagaatc agacgctacc tgcccatcgg ccgtcgatca
     5941 gttgacggtg atcttcttct ggttgtcgcc cgctttcttg ggcagctcca gattcaatac
     6001 gccgtcctcg tatcttgcct ttgtattggt ctcgtcggcg gcttgcggca acgtgaacga
     6061 gcggaactgc ctgccgcagt agcattcccg gtgcacgatc ttttcaccgt tctttttttc
     6121 ttctttttcc ttctttgttt cggcgctgat ggttacctgg tcgccgttca ccgagacact
     6181 gatgtcttcc ttgttgaagc cgggcatttc ggcgcgtacc ttgtagacct tgtcgtcctc
     6241 ggtgatatcg atcggcatat ggaggtcggc catcggattg agcgacatcg gggtagggaa
     6301 gaaccccgtc aacagatcat ccatgtcacg tccaaatggg ttgaaacgtg tcagtccgcg
     6361 gaacgggttg taacgagcaa ggtcagtcat gattcttctc cttcagtggg tcaagaaatt
     6421 tcttcgttcc tcttgctgcg tcaaatgcac aaataattca atgcgttact tcgacgagaa
     6481 ctttcagtac gccaccggcg actcgcccgt tcgttgccaa gcgaagctag agcaagccga
     6541 ggcgatatac gactacaagc ttatgcacca acgaagaaaa ttaaatacgt gcgatcactt
     6601 actggtttcc cggtatgccc gcgtctctac tcgggaacga cctgttattc gccgtttcat
     6661 gctgctagac tgaacattgc tagcagccct ccgggaccgg aaggtgccag ccaggttcat
     6721 acgttcgtga gttggaacat caagaggtcg ccgatgatga acgaaaatga tcgattcgca
     6781 cgacaggata aattccgtca accggtgtcg gtagccgcga gatccacagt aatagaggaa
     6841 cacctgccgg aagatctgac gggactgtcg tcggatgaga tcggggagat ggtgcttgag
     6901 ctgagggcgc atcagatcga gctggagatg cagaacgaga atctgcaccg gactcaggcc
     6961 gccttggatg cgtcgcgagc cctttactat gacctctaca atctggctcc ggtaggttat
     7021 tgcaccctta gcgaggccgg cgtgattctg gaggccaatc accgcatcgc cgcgttgctg
     7081 ggaacgatgc gaggcacact gatcaagcag cccatcttcc gcttcatcca tcgcgaggat
     7141 caggacgtct tttactttca ccgaaaacgg ctgcttgaaa gcagtcagcc ccagcagtgt
     7201 gagctgcgaa tgttgaccgt gggcggcaca cttctctgga tgcatctgaa tagcaatgcg
     7261 acgcgcgatg atgctggcac ggtcatgctg cgcattaccc tgaacgacat cacggaacgc
     7321 aagcgatttg aggatgaact ggttgccgcc aggaagttgg cggaacaggc cgtgctcgtc
     7381 aaatcacgct tccttgccgc cgcaagtcat gacctgcgac agcctttgca ggcgatcaac
     7441 ctgttcaatg acgcattgat cgggaccggc ctcagtgacg agcagaaaca aatcagcgga
     7501 cacctgtcgc aatcgatttc ttcgctgaac gaactcttga acgttctgct ggacttgtcg
     7561 aaacttgatg ccggcattat caaagcgaac ccggaggtca tcgaggtcga gaagttcttc
     7621 ctgagatgcg atgccgagtt ctcgccgatc accaagcgga aacgtttgag tttcaggttg
     7681 tgcttcccgc tcaagcccat ggcgatcatt gcggatcggg agcttttgag gagtctgatg
     7741 ggcaatctca tcttcaatgc catcaaatac acccaacagg gcggggttct cgtagcaatt
     7801 cgccggcgtg ggaccaaggc gctcattcag gtatgggata ccggaatcgg catcccggca
     7861 gaacatatcc acagcatctt tgaggaatac tttcagatcg agaatccgga aagggtcaga
     7921 agaagaggcc tcgggctggg ccttgccatt gtcagacgcc aggcgaaatt gttgggaacc
     7981 gaggtggcat gccgctcgcg tccgggcaag gggagtgttt tcgaattcag cctgccactg
     8041 gccgtcgagg cacaggagga ggtaccgcct cagaacgcga tagatcgagc catagtcgat
     8101 gcatacaaag gtcgtcgcat tgtcgtggtc gaggatgatg ccatggtcgc caatgcaatc
     8161 aagctttcgc tcgaatcagt cggcatgtgc gtcgctgtct ttcaaaatgc cgaagaggcg
     8221 ttgtctgatg cggaaaccct gaaagcggat ttctacgtaa gcgactatcg acttcccgga
     8281 ttggatggcg agcggtttct ggaaaatatt ctgaagcgtt ccacgaaacc attcagggcc
     8341 gtgctgctga ccggggatac aaccccgggc tggatcgaaa aggcgagaag cttgcgctgg
     8401 ccggtactgt tcaaacccat cgatctgccg cagcttttga cgacaatcca gtcccaggca
     8461 gcgagtacat aaaatggccg caaaagacac cgtatttgta atcgatgatg attcagccat
     8521 tcggaatctt ctggcacgca tgcttgaagg tcgcggcatc aacgtcgaat gtttcgagag
     8581 cggagaggac ttcctgcaag tatgccgctg gttgccgcgc agttgtgcca tcgtcgatat
     8641 gaacctgccc ggtctggacg gcacgcagat acaggccgaa ttggcgaggc gcgatatttt
     8701 gctgccgatc gtctttctca ccggtcacgg cgatattcca atgagcgttc gcgcgatcaa
     8761 ggccggtgct tccgactttc tgagcaagcc ggtcagcagc gcggcactcc tggccagcgt
     8821 ggaggcggca ttgaacgaaa gtgaaaggct gaattcgcgt atcggcgaca cggtcgatgc
     8881 cgctgtccgt ctggccagtt tgaccgaacg cgagcaggac gtgctgcgtc tggcggtcga
     8941 aggcctggca aacaaagtca tcgcgcgaga acttggcatc agccaccgca ccgtggagat
     9001 ccacaagtcg cgaatcatgc agaaaaccgg cgcaacatca ttgcttgagc tggcacgcct
     9061 ctacgactca tcgattgaga cctccaattg aggcgaaaac cgttatcgtt tctgttgtgg
     9121 tttcctgaaa ctcatcccgg tgccgaactg cggatcaggt gatcgaaagc gctgagagcg
     9181 gccttggcac cttcacccat ggcaatgatg atctgcttga acggcacagt cgtgcagtcg
     9241 ccggcggcaa agacgccggg cagcgaagtc tggccacgcg catcgacctc gatctcgccg
     9301 tgtgctgaca atccaattgt tcccttgagc cagtcggtat tgggcagcag accaatctgc
     9361 acgaaaatgc cttcgagcgg aatggtgtgg acttcgtcgt ttgtccgatc cttatagatc
     9421 aggccattga ccttttcgcc attgcccgtc gcttcggtag tcagcgcgga ggtgatgacg
     9481 gtcacgttgg gcaagctgaa gagcttcttc tgcaacacgg cgtcggcgcg tagtgccttg
     9541 tcgaattcga gcagggtgac gtgggcgacg acaccggcaa gatcaatcgc cgcctcgacg
     9601 cccgagttgc cgccgccgat caccgccaca cgcttgccct tgaacagcgg accatcgcaa
     9661 tgcgggcaat aggcgacgcc gcgaccgcga tactccttct cgcccggaac gttcatttca
     9721 cgccagcgcg cgccggtgga aatgatcacg gatttgctct tcagtgtcgc cccgctggcc
     9781 agccgaactt cggtgaggcc gttcgagccc ggaaccagag cctcggcacg ttgcagattc
     9841 atgatgtcga cgtcgtactc gcgcacgtgc tgttccagcc cggccgagag cttcgggccg
     9901 tcggtttcct tgaccgagat gaaattctcg atggccagcg tatcgagcac ctgaccgccg
     9961 aagcgttcgg caacaacacc ggtgcgtacg cccttgcgcg ccgcgtagat agcggcagct
    10021 gcgccggccg gcccgccgcc gacaatcagc acgtcgaagg cctccttggc ggcaagtttt
    10081 tcggcttcgc gcgcaacggc gccgatgtcg atcttggcga tgatctcctc aagcgtcatg
    10141 cgtccttgcc caaagggttc accgttcagg aaaaccgtcg gtacggccat gatcctgcgg
    10201 tcgtttacct cgtcctgaaa caaggcgccg tcgatcatcg tatgactgat gccgggattg
    10261 agcacggcca tcagattgag cgcctgaacc acgtccgggc agttgtggca ggacaacgaa
    10321 atgaaggtct cgaagtggaa gtcgcccgga atggcgcgaa tctgctcgat caactcggcc
    10381 tcgaccttgg gtgggtgacc accggtctgc aacagggcca gcacgagcga agtgaactca
    10441 tgccccatcg gaaggccggc gaagccgatg cgcgccggtt cgccgggacg agctacggca
    10501 aacgagggtt tgcgcgtgtc gacgccgtcg ttgcgtacac cgaccttgtc ggaaagcagc
    10561 gcgatgtcgt ccagcaagga ccgcatttcc tgcgccttgt cgctgtcgtc gagcgtagcg
    10621 accagttcga tgggtcgctg aagtcgctcc agataagcct tgagctgggc tttgaggttg
    10681 gcgtcgagca tttcttgttc tcccaattgg tattgcggat tgagccacga agggtgagcc
    10741 cttcgtggcc tgactgccct gatctatatc tttccgacca gatcgagcga tggcgccagc
    10801 gtcgcctcac ccggcgtcca cttggccggg cagacttcgc ccggatgtgt ggcgacatac
    10861 tgtgcggcct gtatcttgcg cttcagttcc ttggcatcac ggccgatgcc cagatcgtga
    10921 atctcgcaaa ccttgatcac gccttcgggg ttgatgacga aggtgccgcg cagggccaga
    10981 ccttcttcct cgatcatgac gccgaagttg cgggtgattg cgccggtcgg atcgccgatc
    11041 atcgggtaat ggatcttctt gatcgtctcc gaagcgtcgt gccaggcctt gtgcgtgaag
    11101 tgcgtgtcgg tcgacaccga atacacctcg acgcccatct tctggaaatc ggcgtattcg
    11161 tcggcaagat cgcccagctc ggtcggacaa acgaaagtga aatcagccgg ataaaagaac
    11221 acgactgacc acttgccgcg caggtcggcg tcggatactg gaccgaactt gccattgtgg
    11281 tatgcggtgg ccttgaaggg caggatttcg gtattgatca atgatttcat tttgaggact
    11341 ccttatgtgc gacgggttga aaatcagact acggcctcat ggtagtgttg cgaccaaaat
    11401 taatctaatt gattgtttta atttattgga taggcaaaag ctattagtcg atccttttcg
    11461 cccacttgcg ccctcgggta caatgtcggc ctttcaaatc aacgtttttc ggggttcctg
    11521 agtgctcgta gtcgccaaca tcaccatgca gttcggggcc aagccccttt ttgaaaacgt
    11581 ttcggtcaaa tttggagagg gcttccgcta cggcctgatc ggggccaatg gttcgggcaa
    11641 gtcgaccttc atgaagatac tcgacggcga gctggaagcc actgccggca atgtctcgaa
    11701 agacgcacac gagcgtatgg cctacctgcg gcaggatcag tttgccttcg aagaccagcg
    11761 ggtgatcgac gtggtgatga tgggccacca ggaaatgtgg gcctgcatgc aggagaagga
    11821 tgcgatctac gttaacccgg atgcgagcga agaggacttc atgcatgccg ccgaactcga
    11881 aagccgcttt gccgaatacg gcggctacac ggccgaagcg cgtgccgggg agctgctgct
    11941 cggtgtcggc attccgagcg aacagcactt cggcccgatg agcgaagttg cgccgggctg
    12001 gaagctgcgc gtcctgttga cgcaggcact gttctccgac cccgacatcc tgctgctcga
    12061 cgaaccgacc aacaacctcg acatcgccac catccgctgg ctggaaaacg tgctcaacga
    12121 ccgcaacagc acgatgatca tcatttccca cgatcgccac ttcctgaatc aggtgtgcac
    12181 gcacatggcc gacctggact acggcaagat caccgtgtat ccgggcaact acgacgactt
    12241 catggaagcc tcgtcgcagg cgcgccagca gcagcaaaac gccaatgcac gcgccaagga
    12301 gcgcatctcc gaactacagg atttcgtgcg gcgcttctcg gccaacaagt ccaaggccaa
    12361 gcaggccacg tcgcgcgtca aactgatcga aaagcttcgc ccggaggacg tcaaaccctc
    12421 ctcgcgccag tacccgtgga ttcgtttcga ttacgacgag aaagaaaaac tgcatcgaca
    12481 ggccgcagaa gtcgaaaacc tcacctttgc ttacgcaggc agtgagcaca agatcttcga
    12541 cgactttact ttcacgctca atgccggcga ccgcgtcgcg gtgatcggcg aaaatggcgt
    12601 tggcaaatcg acttttctaa agctgatgat gggcgaactg cagccgcagt ccggcagcat
    12661 caaatggacg gaaaaagcac gtcgcggtta ctacgcgcag gaccacgcgg acgacttcaa
    12721 aggcgacacc agcctcgtcg aatggatcgc cggttatgcc cgcgccaatg cggccgaggg
    12781 tgaggatctg gaaacgctga ttcgcggaac gctcgggcgt ctgctgttct cgggggacga
    12841 agtgcgcaag gcggtgaaag tcatctccgg cggcgaacag gggcgcatga tcttcggcaa
    12901 actgatgctg atgaaaaaca acgtgctggt gttggacgag ccgaccaacc acctggacat
    12961 ggaatcgatc gaatcgctca atacggcgct ggaaaagttc aagggcacgc ttctgtttgc
    13021 gtcgcacgat cgcgagttcg tatcatcgct ggccacgcgc gtactcgaaa tccggctgga
    13081 tggcacggtg gtcgactacc tcggcaacta cgaggagtac ct
//
LOCUS       DAORPM010000032        13091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_979_length_13091_cov_14.250767, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000032 DAORPM010000000
VERSION     DAORPM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_979_length_13091_cov_14.250767"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2612
                     /gene="metH"
                     /locus_tag="PLD53_02810"
     CDS             <1..2612
                     /gene="metH"
                     /locus_tag="PLD53_02810"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRC59866.1"
                     /translation="SKAFARMILEGRLDDALAVARQQVENGAQVIDVNMDEAMLDSKA
                     AMDRFLKLIASEPDISRVPIMIDSSKWDIIETGLKCIQGKGIVNSISMKEGEAAFVQQ
                     AKLARRYGAAVIVMAFDEAGQADTYARKTEICKRAYELLLSIGFPPEDIIFDPNIFAI
                     ATGIEEHNNYAVDFIEACRWIRANLPFAQISGGVSNVSFSFRGNDPVREAIHTVFLYH
                     AVKAGLSMGIVNAGMLGVYDDLEPALRNKVEDVVLNRSPEAGEALVEFAQSVKEGKAK
                     DSGPDLSWRELSVEARLTHALVKGITDFVVADTEECRAALEAAGQPPLSVIEGPLMNG
                     MNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPYVEAEKLRTGAASKGKIVIATVKG
                     DVHDIGKNIVGVVLGCNGYDIVDLGVMVSCDKILHAAKEHGAQAIGLSGLITPSLEEM
                     SHVAAEMQRLGFCDGDHGEAIPLLIGGATTSRAHTAIKIAPHYNGPVVYVPDASRAVG
                     VVTKLLSIDNATGFKAEVAADYEKVRTQHANKKGVQLVSLEVARDNGFAWNSDPAYRP
                     VAPNKPGLHVLRNVDLATLVEYIDWGPFFQTWDLAGGYPKILDDAVVGETARSVFADG
                     KAMLEKIIADQHANPWITANAVFGIYPANAVGDDIEIYRDESRKEKLMTWHSLRQQHE
                     RPAGKPHQCLSDFIATKGTADWIGAFAVTAGLGIEKKLAEFEAAHDDYHAIMLKSLAD
                     RLAEACAEWLHAQVRRHYWGYAANESLDNDALIREQYKGIRPAPGYPACPDHTAKGPL
                     FDVLDAPGNAGMGITESFAMTPAAAVSGFYLAHPQAKYFAISKIGRDQLEDWAQRTGM
                     STVEAERWLAPLL"
     gene            2627..3664
                     /gene="murB"
                     /locus_tag="PLD53_02815"
     CDS             2627..3664
                     /gene="murB"
                     /locus_tag="PLD53_02815"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRC59867.1"
                     /translation="MLPERLPSFVEREAHLASRNTLALPGRAALYAEITSVGQLALLS
                     GGVASRCFILGGGSNLVLTGDFDGLVLHMAIGGRELVAEDDDAWYVRAGAGENWSDFV
                     GWTLAHGWPGLENLSLIPGTVGAAPIQNIGAYGLELAERFHSLVAFDMNSGQTVRFDA
                     KACRFGYRDSVFKQEGWHLEGRFVITEVSFRLPKIWQARTLYADVANELAARMISDPD
                     AQQVAAAVVAVRQRKLPDPALVSNAGSFFHNPVVDVTTADRLLVAHPGLPIYPQTDGR
                     CKLAAGWLIERAGWKGRYLGPVGMYEKQALVLVNRGGATGADVSMLMQAVQRDVREKF
                     GVELTPEPIIL"
     gene            complement(3702..4550)
                     /locus_tag="PLD53_02820"
     CDS             complement(3702..4550)
                     /locus_tag="PLD53_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59868.1"
                     /translation="MPLDSVDPLDADELMNFAERIEQLSPADAEWVGSLFQECMRARM
                     REAELLSGLTEAGATESTEFDAQLAQVALDAAEWLKTLWNVGYMGAGSFPSQPRSAFP
                     LIELEDVIKSALFARIREGKRPLPFPPPTRHGLPWHDLVESAEITYDVAAEIVRDDQG
                     QSIGAIVEACPDWQLIEEITKDREYIIQHRGLGPLFRLRIEHPETSPTSTLRREPPRW
                     TRQIRLQERGGFRSYTLEWPQEEGGMQSISLRAATWERAESEAGYWIVTKHPEMYGQV
                     KFEKAE"
     gene            4728..5465
                     /locus_tag="PLD53_02825"
     CDS             4728..5465
                     /locus_tag="PLD53_02825"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HRC59869.1"
                     /translation="MIGKTVLVTGAAKRVGCSIARELHASGANIMVHFRSMADAAEAL
                     TAEFNSARPRSALCQQADLLNIDALSALVTTTVAHFGRLDALVNNASSFFATPFGEIN
                     EAVWDDLVGSNFKAPLFLTQAAAPHLKTVHGAVVNITDIHAERPMAGFSLYCAAKAGL
                     LGLTRALAIELAPEVRVNAVAPGPILWPDDSTFSSETRDRIVAHTLLKRAGSPQDVAR
                     TVRFLIEDAPYVTGQVISIDGGRSAHL"
     gene            5508..6395
                     /gene="ttcA"
                     /locus_tag="PLD53_02830"
     CDS             5508..6395
                     /gene="ttcA"
                     /locus_tag="PLD53_02830"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HRC59870.1"
                     /translation="MENSNTLQRLRKRLESDVGKAIADYNMIENGDTVLVCLSGGKDS
                     YAMLSTLMALRKRAPVDFRLIAMNLDQKQPGFPADVLPDYLSGLGIEYRIVEQDTYSI
                     VKEKIPEGKTTCSLCSRLRRGVIYRTARELGANKIALGHHRDDIVHTLFLNLLFGGKL
                     KAMPPKLVTDDGTLVVIRPLAYCNERDIARFARGMAYPIIPCNLCGSQNNLQRQKIRE
                     MMADWDRRYPGRTESVFTALQNVVPSHLADNSLFDFKGLQVGAELADMDGGDTVFDSP
                     QFAQDVSPTRAMKLQVVSA"
     gene            6562..7464
                     /locus_tag="PLD53_02835"
     CDS             6562..7464
                     /locus_tag="PLD53_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC59871.1"
                     /translation="MNIADQHLSVLFAGVAGSVRLHEKLGGDEAARAVDRCLTRMERA
                     VEAFGGRVIKRVGDEFMVVFDLPDEAFQAAIEMRQRVADLPPVSGVKLAIRIGFSHGL
                     VSEADGNIVGETVVEAAGLAGLAMPGQVLASHQAHAALSPALKKMTRDLGSASTKGQS
                     PKGIAFEVVTQGTAAPEIKKPGDPEERQGDKPRDGKLHLRYAGKHITLNDSKPVITMG
                     RDPKCDVVVHDRRASRNHARIELRGERIVVIDTSTNGTYVSLHGKPELFLRKEECGLY
                     SKGLICFAGSATSTGADFAEFDLS"
     gene            complement(7534..7815)
                     /locus_tag="PLD53_02840"
     CDS             complement(7534..7815)
                     /locus_tag="PLD53_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HRC59872.1"
                     /translation="MSNAFAADQVKKTPSPAQQAQQDKMKACNADAGNKALKGDERKK
                     FMSECLSSKPASATQQDKMKACNADAGNKALKGDERKKFMSECLKADKQ"
     gene            complement(7906..8850)
                     /gene="hprK"
                     /locus_tag="PLD53_02845"
     CDS             complement(7906..8850)
                     /gene="hprK"
                     /locus_tag="PLD53_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRC59873.1"
                     /translation="MIPTRTLSITQLYTDHLEKLKLTWIAAVGVERQVVLKDQGIYGP
                     DVVGHLNLIYSHRVQVIGKAEQRWAERVGEERWQRQIDDLMAATPPALIVADGLKIPS
                     GAKEMCEATQTPLFVTPKECSVVIDLLHIYLARRFADTVSAHGVFMDVYGMGILITGD
                     SGVGKSELALELVTRGHGLVADDVVELARTAPTTIEGRCPSMLRDYLEVRGLGLLNIR
                     TIFGETAARRKMKLKLVVHLQEFAAGEDSPRLPLDSQTQEILGIPVRKVVFHVAPGRN
                     LAVLLEAAVRNCILQMRGIDSMSEFINRQQQAVLDDDF"
     gene            complement(8863..9309)
                     /gene="ptsN"
                     /locus_tag="PLD53_02850"
     CDS             complement(8863..9309)
                     /gene="ptsN"
                     /locus_tag="PLD53_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HRC59874.1"
                     /translation="MSQITQLLAPKNIVLDLDASSKKRVFEHVGLLFENNQGIASSAV
                     FDSLFSREKLGSTGLGQGIAIPHGRIKGLKEASGAFLRLAAPVPFDSPDGKPVSLLFI
                     LLVPEQATEQHLQILSELAEKFSNPTCREALSKAESTDEIRLLFAN"
     gene            complement(9437..9760)
                     /gene="raiA"
                     /locus_tag="PLD53_02855"
     CDS             complement(9437..9760)
                     /gene="raiA"
                     /locus_tag="PLD53_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRC59875.1"
                     /translation="MNLQISGHHLEITSAIHDYVTGKLERVTRHFDNVIDVNVILSVE
                     KLKQKAEVTVHLSGKDVFVESIDEDLYAAVDSLVDKLDRQIQKHKQKVKNHHRGQRIS
                     HIVQD"
     gene            complement(9896..11323)
                     /locus_tag="PLD53_02860"
     CDS             complement(9896..11323)
                     /locus_tag="PLD53_02860"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRC59876.1"
                     /translation="MKPSLQLKLSQHLALTPQLQQSIRLLQLSTLELEQELEKYLQEN
                     PLLERMEEEYAPPLPGTNDTDSDTAEASVNETDTATSSADDESWLGDEEDSNYQSASS
                     SGSFDDDDGDYQDVQVATTSLREHLTAQLGLMSIPDRDRTLVQCLIEALDDDGYLTQS
                     LEDLAETLPAELEIEPEELQIALNHLQCFEPTGVGARNVAECLALQLETQPADDTQAL
                     ALNIVRNNLDLLANRDFVKLKKLIECDDEQLRLAQSLIRSLNPRPGSKYAALDARYIT
                     PDVVVRKLRGQWTVNVNSDAFPRLRINSLYAQILSKQRGSGLAGQLQEARWLIKNVQQ
                     RFDTILRVAQSIVDRQRQFFDHGEVAMRPLVLREIADLLGLHESTISRVTTQKYMATP
                     RGIFELKYFFGSHVATETGGACSATAIRALLKQLIGSEEQKRPLSDSQLSAILGQQGI
                     VVARRTIAKYREALSIPPVKLRKTL"
     gene            complement(11401..12156)
                     /gene="lptB"
                     /locus_tag="PLD53_02865"
     CDS             complement(11401..12156)
                     /gene="lptB"
                     /locus_tag="PLD53_02865"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRC59877.1"
                     /translation="MISETNVNPSTKSLLSTHNLKKRFKKRTVVEDVSFEVRSGEVVG
                     LLGPNGAGKTTCFYMVVGLIPCDGGDVHLDDRKITHLPIHKRAQLGVSYLPQEASVFR
                     KLTVVENIQAVLELQNLPPDEIEFRAESLLEELHISHIRSSSAASLSGGERRRVEIAR
                     ALSTEPRFILLDEPFAGVDPIAVLDIQKIIHFLKERDIGVLITDHNVRETLGICDHAY
                     ILNDGSVLAAGRPDEIIYNENVRKVYLGENFRL"
     gene            complement(12157..12603)
                     /gene="recX"
                     /locus_tag="PLD53_02870"
     CDS             complement(12157..12603)
                     /gene="recX"
                     /locus_tag="PLD53_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HRC59878.1"
                     /translation="MSSTLRERALRLLARREHARAELARKLSPHADSAEELEALLDDL
                     VARRLLSDERYVEMRMNSRAARFGNARLAYELRTQGISEELMDGALAASGPELTRARL
                     VWLGKFGNKTGPSDAAGRARQTRFLMSRGFPGDIIRRVLRGDFEDD"
     gene            complement(12610..>13091)
                     /locus_tag="PLD53_02875"
     CDS             complement(12610..>13091)
                     /locus_tag="PLD53_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HRC59879.1"
                     /translation="NINRTNTLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLD
                     IRRIGGIKKGDEVIGNETRVKVVKNKVAPPFREAIFDILYGEGTSREGEIVELGVIHK
                     LVEKSGSWYAYGGDKIGQGKDNAREYLKANPQIAEEIEAKIRAAVNVPSPKPFNAAE"
BASE COUNT         2674 a         3552 c         3894 g         2971 t
ORIGIN      
        1 gctcgaaagc cttcgcccgc atgatcctcg aaggtcgcct tgacgatgcg ctggcagttg
       61 cccgccagca ggtcgagaac ggcgcacagg tcatcgacgt caatatggac gaggcgatgc
      121 tggactcgaa ggcggcgatg gaccgctttc tcaaactgat cgcttccgaa cccgacattt
      181 ctcgcgtgcc gatcatgatc gactcgtcga aatgggacat catcgaaacc ggcctcaagt
      241 gcatccaggg caagggcatc gtcaattcga tctcgatgaa ggagggtgaa gccgcgttcg
      301 tgcagcaggc caagctggcg cgtcgttacg gtgccgcggt tatcgtcatg gctttcgacg
      361 aagccggcca ggccgatact tacgcgcgca agacggagat atgcaaacgc gcctacgaat
      421 tgctgctgag catcggcttc ccgcccgagg acatcatctt cgacccgaac atcttcgcca
      481 ttgccacggg tatcgaggaa cacaacaact acgctgtcga tttcatcgag gcctgccgct
      541 ggatccgcgc caatttgccc ttcgcccaga tctccggcgg cgtctccaat gtttccttca
      601 gttttcgcgg caacgatccg gttcgcgagg cgatccacac cgtttttctc tatcacgcgg
      661 tgaaggccgg gttgtcgatg ggcatcgtca atgccggcat gcttggcgtt tatgacgacc
      721 tggaaccggc gctacgcaac aaggtcgaag atgtagtgct caaccgcagc ccggaggctg
      781 gcgaggcgct ggtcgaattc gcgcaaagcg tgaaggaagg caaggccaaa gacagtggcc
      841 cggatctttc ctggcgcgaa ctgtcggtgg aggcgcgtct cacgcatgcg ctggtcaagg
      901 gcatcaccga ttttgtcgtt gccgacaccg aagagtgccg cgccgcgctc gaagccgccg
      961 gccagccccc gctgtcggtc attgaaggtc cgctgatgaa cgggatgaac gttgtcggcg
     1021 acttgttcgg cgcgggcaag atgttcctgc ctcaagtcgt caagtcggca cgtgtcatga
     1081 aacaggcggt agcgcacctg attccctatg tcgaagccga aaaactgcgc accggcgcgg
     1141 cgagcaaggg aaagatcgtc attgccacgg tgaagggcga cgtccatgac atcggcaaga
     1201 acatcgtcgg tgtcgtgctc ggctgcaacg gctacgacat cgtcgatctc ggcgtcatgg
     1261 tttcctgcga caagatactg cacgcggcaa aggaacacgg tgcgcaggcc atcggcctgt
     1321 ccggcctgat cacgccctcg cttgaggaaa tgagccatgt cgccgctgaa atgcagcgcc
     1381 ttggattttg tgacggagac catggcgagg ccatcccgct gctgatcggt ggtgcaacaa
     1441 cgagccgcgc acacacggct atcaagatcg cgccacatta caacgggccg gtggtttacg
     1501 tgccggatgc gtcgcgcgcg gtcggtgtcg tcaccaaact gctttcgatc gacaatgcca
     1561 ccggtttcaa ggccgaagtc gctgccgact acgagaaagt gcgtacccag cacgccaaca
     1621 agaagggagt tcagcttgtt tcgctggaag tcgcacgcga caacggcttc gcttggaata
     1681 gcgatcccgc ctatcgaccc gttgcgccga acaaacccgg cttgcatgtg ctgcgcaatg
     1741 tcgatctggc aacactggtt gaatacatcg attggggtcc gttcttccag acctgggatc
     1801 tggccggagg ctacccgaag attctcgacg acgccgtcgt tggcgagacg gcacgttccg
     1861 tattcgccga cgggaaggcg atgctcgaaa agatcattgc cgatcagcac gccaacccgt
     1921 ggatcaccgc caacgcggtt ttcggcattt atcccgccaa tgcagttggc gacgacatcg
     1981 aaatctaccg cgacgaatcg cgcaaggaaa agctgatgac ctggcatagc ctgcgccagc
     2041 agcacgagcg gccagccggc aagccgcatc aatgcctcag cgatttcatt gcaacaaaag
     2101 gcacggccga ctggatcggt gcctttgccg tcaccgctgg ccttggcatc gagaagaagc
     2161 tggccgaatt cgaagcggcg catgacgact accacgcgat catgctcaaa tcgcttgccg
     2221 accgtctggc cgaagcctgc gccgagtggc tgcatgcgca ggttcgacgc cactactggg
     2281 gctatgccgc gaatgagtcg ctcgacaacg acgcgctgat tcgcgagcaa tacaagggca
     2341 tccgtccggc gccggggtac ccggcctgcc cggaccacac ggccaaggga ccactcttcg
     2401 atgtgctcga tgctccgggg aatgccggca tgggaattac cgaatccttc gccatgaccc
     2461 cggccgccgc tgtatcaggc ttctacctgg cacacccgca agcgaagtat tttgcgatca
     2521 gcaagatcgg ccgcgatcag ctggaggatt gggcgcagcg cactggcatg agcactgtcg
     2581 aagccgaacg ctggctggca ccgctgctct gagttcagcc gaagcgatgc ttcccgaaag
     2641 gcttcctagt ttcgtagagc gcgaggctca tctcgcttcg cgcaacacgc tggcgttgcc
     2701 tggccgtgct gccctctatg ccgagatcac ttctgtcggt cagctcgcct tactctccgg
     2761 gggcgtcgct tcacgctgtt tcattctcgg cggcggcagc aacctcgtgc taaccggtga
     2821 tttcgatggg ctcgttttgc atatggcgat tggcgggcgg gaactggtcg ccgaggatga
     2881 cgatgcctgg tatgtgcgcg ccggcgcggg tgagaactgg agcgactttg ttggctggac
     2941 gctggcacac ggctggccgg gtctggagaa cctgtcgctg atccccggca cggtcggcgc
     3001 tgcgccgatc cagaatattg gcgcatacgg gcttgaactg gctgaacgct ttcattcgct
     3061 tgtcgctttt gacatgaata gtggccagac cgtgcgcttt gacgctaaag cttgccgttt
     3121 cggctatcgc gacagtgtgt tcaagcagga aggttggcat cttgagggcc gcttcgtcat
     3181 caccgaagtc agcttccgcc tgccgaaaat ctggcaagcg cgcactctct atgctgatgt
     3241 tgccaatgaa cttgcagcac gaatgatcag cgaccctgat gcacagcagg tagccgcggc
     3301 cgttgttgcc gtccgccagc gcaaactgcc cgacccggcg ctggtttcca atgccggcag
     3361 tttctttcat aacccggtgg tcgatgtgac gactgccgac cggctgttgg tcgcccatcc
     3421 cggtctgccg atctatccgc aaaccgatgg tcgctgcaag ctggctgccg gctggttgat
     3481 cgagcgggcc ggctggaaag gcaggtatct cggcccggtc ggcatgtacg aaaagcaggc
     3541 gctggtgttg gtcaatcgtg gtggcgccac gggtgccgat gtgtcaatgc tcatgcaggc
     3601 ggtgcagcgt gatgtgcgcg agaagttcgg cgttgaattg accccggagc cgatcattct
     3661 ctgagctggc gtcagcggaa ttcctgctgt ttcaggtggg gctattctgc cttctcgaac
     3721 tttacctgcc cgtacatttc ggggtgcttg gtgacgatcc agtatccggc ttcggactcg
     3781 gcgcgttccc aggttgccgc acgcagcgag atgctctgca tgccgccttc ttcctgcggc
     3841 cattcaagcg tgtaactgcg aaagccgccc cgttcctgca agcggatctg gcgtgtccag
     3901 cgcggcggct ctcggcgcag tgtcgaagtc ggactggttt ccggatgttc gattcgtagt
     3961 ctgaaaagcg gccccaagcc gcgatgctga atgatgtact cacgatcctt tgtgatctct
     4021 tcaatgagtt gccagtcggg gcaggcttcg acgatagcac cgatggattg tccctggtcg
     4081 tcgcgaacaa tctccgctgc aacgtcataa gtgatttccg cactctcgac aagatcatgc
     4141 cagggtaggc cgtgtcgggt cggtggcggg aagggaagcg gccgtttccc ttccctgatt
     4201 cgggcaaaaa gcgccgattt gataacatct tccagttcga tcagggggaa ggccgagcgc
     4261 ggttgcgaag gaaaactgcc ggcgcccatg tagcccacat tccacagtgt cttcaaccac
     4321 tccgctgcgt caagtgccac ttgcgccagc tgggcatcaa attcggtgct ttccgttgca
     4381 ccggcttcag tcagcccgga cagtaattcg gcctcgcgca ttcgtgcgcg catgcactcc
     4441 tgaaacaaac tgcctaccca ttcagcgtca gcgggagaaa gctgctcaat gcgttcggcg
     4501 aagttcatca attcatcggc gtcaagcggg tcgacagaat cgagtggcat ttcatcattc
     4561 atcatggggt tcgtgtcccg ggttgttgag gcaagtttaa cccctcgcgt cagcttcgga
     4621 tagcggccct gggagcgtcg gagagcgacc ttgtcccggc ggctggcgtg aaaatccgtt
     4681 agaatcctcc tcctggtgag cagtggtctg gaactctgga gtcagtagtg atcggcaaaa
     4741 cggtattggt gaccggtgca gctaagcggg ttgggtgctc aatcgcccga gaattgcatg
     4801 cttcgggtgc caacatcatg gtgcatttcc gtagtatggc ggatgcggcc gaagcgctga
     4861 cagccgaatt caattctgcg cgaccaaggt ctgctctttg tcagcaggcc gacctattga
     4921 atatcgacgc actatccgcc cttgtgacaa ccacggttgc acattttggg cgcctggacg
     4981 cgctggtcaa caacgcgtca agtttttttg caacaccgtt tggcgagatc aatgaggcgg
     5041 tctgggacga tcttgtcggc agcaatttca aggcaccact atttctgacc caagcggcag
     5101 cgccacatct gaaaacggta catggtgctg tagtcaacat caccgatatt catgccgaaa
     5161 ggccgatggc cggattttcg ttgtactgtg cagccaaggc ggggctgctc gggcttacgc
     5221 gtgcgctggc cattgaactg gcgccggaag tgcgtgtcaa tgcagtagcg ccggggccga
     5281 tactgtggcc tgacgacagt acatttagca gcgaaacgcg tgaccggatc gtcgcacata
     5341 cgttattgaa acgcgccgga agcccacagg atgttgctcg caccgtgcgt ttcctgattg
     5401 aggacgcacc ctatgtgact gggcaggtta tcagcatcga cggtggacgt agcgcacatc
     5461 tgtaacggtt ccaacacaat cgtatcgaat cagttcgtag aggtccggtg gaaaattcaa
     5521 acacgcttca gcgcttgagg aaacgtcttg agagcgacgt cggcaaggcc attgccgatt
     5581 acaacatgat cgagaatggc gacactgttc ttgtctgcct gtccggcggc aaggattcgt
     5641 atgccatgct ttcgacgctg atggccttac gcaagcgcgc gccggtggat tttcggttga
     5701 ttgcgatgaa tctggatcag aagcagccgg gttttcctgc cgatgtgctt ccggattatc
     5761 tgtccggcct ggggatcgag tatcgaatcg tcgagcagga tacctattca atcgtcaagg
     5821 aaaagatccc tgaaggaaag acgacgtgct ccttgtgctc ccgcctgcgg cgtggggtga
     5881 tttaccgtac cgcgagggaa ctgggtgcca acaagatcgc cctcggccat catcgagacg
     5941 acatcgtaca caccctgttc ctcaacctgc tgtttggtgg aaaactgaag gccatgccgc
     6001 ctaaactggt caccgatgac ggtacgcttg tcgttatccg cccgctggct tactgcaacg
     6061 agcgcgacat cgcgcgcttt gcgcgcggca tggcgtatcc gatcattccg tgcaatttgt
     6121 gcggttcgca gaacaacctt cagcgacaga aaattcgcga gatgatggct gactgggacc
     6181 gccgttatcc agggcgaacc gagtcggtat tcacggcgtt gcagaatgtt gtgccatcgc
     6241 atctggcgga caattcgctg tttgatttca aaggactcca agtcggcgcc gagctggctg
     6301 atatggacgg tggtgacacg gtgtttgaca gcccgcagtt tgctcaggac gtatcgccaa
     6361 ctcgggcaat gaaactgcag gtggtcagcg catgaagcgc aaagtgtggt cttttgatat
     6421 catccggaag acgtggccgc atcgtgattc agcggattcg gcgctcgcag aatgggaact
     6481 ggaaaggacg gtgaggattt ggaagaggtt tcttatggag gaactgcgac tgaggttttc
     6541 ggtaccacaa tgcctagtga tatgaatatt gcagatcagc acctttccgt cttgtttgcc
     6601 ggtgtggcag gaagtgtacg gctgcatgaa aagcttgggg gtgatgaggc ggcgcgggct
     6661 gttgatcgtt gtctgacgcg catggagcga gcggtcgagg cttttggtgg gcgggtcatc
     6721 aagcgcgtcg gtgatgaatt tatggtcgtg ttcgaccttc ctgacgaggc atttcaggcc
     6781 gccatcgaga tgcggcaacg cgtggctgac ctgccgcccg tttcgggagt gaagctggca
     6841 attcgaatcg gattttcaca tggattggtc agcgaagctg atggcaatat tgtcggcgaa
     6901 accgtggtgg aggctgctgg ccttgccggg ctggccatgc ctgggcaggt gttggccagt
     6961 catcaggcac atgctgcatt gtcacccgca ctgaaaaaaa tgacgcgcga tcttggctcg
     7021 gcgtccacca aaggccagtc accgaaaggg attgctttcg aagtcgtgac tcagggtacg
     7081 gcagcaccgg agataaagaa accgggcgat cctgaggagc gccaaggtga taagccacgg
     7141 gatggcaagc ttcacctgcg ctatgcggga aagcacatca ctttgaatga cagcaagccg
     7201 gtaattacca tggggcgcga tccgaagtgc gatgtcgtag tccatgatcg tcgagcgtca
     7261 cgcaatcatg cgaggatcga attgcggggc gagcggatcg ttgtcatcga caccagcacc
     7321 aatggcacct atgtttccct gcatggcaaa ccggagttgt tcctgcgcaa ggaggaatgc
     7381 ggtctctaca gcaagggcct tatctgcttt gcgggctcgg cgacgagtac gggtgcggat
     7441 ttcgcagaat tcgacctgag ctgatctagg caggctggat acaaaaaaac gacggtgtaa
     7501 atcaccgtcg tttttttgtc agtcaatcgg cgtctactgc ttgtcggcct tgaggcactc
     7561 gctcatgaat ttcttgcgtt cgtcaccctt gagagccttg tttccggcat cggcgttgca
     7621 tgctttcatt ttgtcctgct gcgtggcgga cgctggcttt gagctaaggc actcactcat
     7681 gaacttcttg cgctcatcgc ccttgagagc cttgtttccg gcatcggcat tgcacgcttt
     7741 cattttgtcc tgctgcgctt gctgcgcggg cgagggtgtt ttcttgactt gatcagccgc
     7801 aaaggcattg ctcatggaaa aggtgatggc aacgagagca atcatgattt tcataggtaa
     7861 ctccttgaca atgtttggaa acgtccgctg attataaacg gcaaatcaaa aatcgtcgtc
     7921 aaggacagcc tgttgctgac gattgatgaa ttcgctcatg ctgtcaatgc cgcgcatttg
     7981 cagaatgcag ttgcgtaccg cagcttcgag caaaaccgca aggttgcgtc cgggcgcgac
     8041 gtgaaaaacg actttgcgta ccggaatgcc gagtatttcc tgtgtctggg aatcgagcgg
     8101 caagcgcggc gaatcttcgc ctgcagcaaa ttcctgcagg tgtacaacca gtttaagctt
     8161 catctttcgt cgcgccgcag tttcgccaaa gatggtgcga atgttaagca ggccgagtcc
     8221 acgtacttca aggtagtcgc gtaacatgct ggggcagcgg ccctcgatgg tcgtaggcgc
     8281 cgttcgtgcc agttcgacta catcgtcggc taccagaccg tgcccacgcg taacgagctc
     8341 gagggcgagt tcgctcttgc cgactccgga atcgccggtg atcagaatgc ccatgccgta
     8401 cacgtccatg aacacgccgt gtgccgacac ggtgtcggca aaccggcgtg caaggtagat
     8461 gtgcagcaga tcgatgacca ccgaacactc ctttggagtg acaaatagtg gggtctgggt
     8521 cgcctcgcac atttccttgg ctccggacgg gatcttcaga ccgtccgcga cgatcagcgc
     8581 gggcggcgtt gctgccatca gatcgtcgat ttggcgttgc cagcgctctt ccccgacacg
     8641 ctctgcccaa cgctgttcgg ccttgccgat aacctgcaca cgatggctgt atatcaggtt
     8701 gagatggcca acgacgtcag ggccgtaaat tccttgatcc ttgagtacga cctgcctttc
     8761 gacacccact gcagcgatcc aggtgagctt gagcttttca agatgatccg tgtatagctg
     8821 agtaatgctg agcgtgcggg tcggaatcat gctgataggc gcttagttgg cgaaaagcag
     8881 gcggatctcg tcggtacttt ccgctttgga gagtgcttcg cgacaggtgg gattcgagaa
     8941 tttttcagcg agttcagaaa ggatttgcag gtgctgctcc gttgcctgtt caggaacaag
     9001 caatatgaaa agaagtgaca ctggctttcc atccggcgag tcgaacggaa caggcgcggc
     9061 aaggcgcaga aatgcgccgc tcgcttcctt taaacccttg atgcgcccgt gcggaatcgc
     9121 gataccctgg ccaaggccgg tcgaaccgag cttttcgcga gaaaaaaggc tgtcgaaaac
     9181 cgcgctgctg gcgatcccct gattgttctc gaaaagcagg ccaacgtgct caaagacacg
     9241 ctttttgctg cttgcatcaa gatcaaggac aatgttcttg ggggctaata attgagtgat
     9301 ctggctcata caggaaggtt gacaagatgg ctgaaaatcg cggcgggcag ttggcgacaa
     9361 tgatgagtga agaaacgcat ggttttgtgt ggcgtgttga cccgacaaga aggccgaacc
     9421 cgccgcacag agtaagtcag tcctggacga tatgacttat cctttgacca cggtggtgat
     9481 tcttgacctt ctgcttgtgt ttttgtatct ggcggtcgag cttatcgacc agactatcaa
     9541 ccgcagcgta gaggtcttcg tcaatggact cgacgaagac atccttgccg gacaggtgca
     9601 cggtgacttc tgccttctgc ttcagttttt cgaccgatag aatcacgtta acatcaatga
     9661 cgttatcgaa gtgacgcgtg acgcgctcaa gtttcccggt aacgtaatcg tggattgccg
     9721 aagtgatttc gaggtgatgt ccactgatct gaagattcat ggtgcggctc cttgtcagag
     9781 ttatagtcgg gtcagcgact gtcgttccct tgccggcatt ctgaggtcgg gtcggggatg
     9841 atggttggtg cggggctggc tttcatcttt tgaggtgacg attctgttgt gcctgctata
     9901 gagtcttgcg tagtttaacc ggcgggatac tgagtgcttc acggtatttt gctatcgttc
     9961 gtcgagcgac aacaatgccc tgctgcccca ggatggcgga caattgacta tcggagagag
    10021 gtctcttctg ctcttcggat ccaattagtt gcttgagcaa tgcacggatc gccgttgccg
    10081 agcaggcgcc gccggtttca gtggcgacat gactgccaaa aaaatacttc aattcgaata
    10141 tgccgcgtgg cgttgccatg tatttttgtg tcgttaccct ggatatggtc gattcatgca
    10201 agcccagaag gtcggcaatt tcacgtagaa ccagcggcct catggcgact tcaccatgat
    10261 cgaaaaactg ccgctgacga tcaactatag actgcgctac tctgaggatc gtgtcaaagc
    10321 gttgttgtac gttttttatc agccagcgcg cttcctgcaa ctgtccagcg agtccggagc
    10381 cgcgctgctt ggagagaatc tgggcgtaca agctgttgat gcgcaggcga ggaaaggcgt
    10441 cggagttgac gttgactgtc cactggccac gcaattttcg cactacgacg tcgggagtga
    10501 tgtatcgtgc atcaagtgct gcgtattttg agccaggtcg agggttgagg ctgcgtatga
    10561 gcgactgggc cagacgcaat tgttcgtcgt cgcattcgat gagctttttg agtttgacaa
    10621 aatcgcgatt ggcaagcaga tcgaggttgt tgcgtacgat attcaatgcg agggcctgcg
    10681 tgtcatccgc cggttgcgtt tccagttgaa gtgcaaggca ctctgcgacg ttgcgagcgc
    10741 caacaccggt tggctcgaag cactgcaagt ggttgagggc aatctgcaat tcctcaggtt
    10801 cgatctccag ttcagcaggc aatgtttctg ccagatcctc aagcgactgg gtcaggtagc
    10861 catcgtcgtc aagtgcttcg atcaggcatt gcaccaaggt acggtcacgg tcgggaatac
    10921 tcatcaaacc gagctgcgct gtcaggtgct cgcgcagcga ggtggtcgcg acctgaacgt
    10981 cctgatagtc gccgtcatca tcgtcaaacg atccggaaga ggaagcgctt tggtagttgc
    11041 tatcttcttc atcaccgagc cagctttcat cgtcggctga ggaggtcgcc gtatccgttt
    11101 cgttcacgga tgcttcagca gtgtcggaat cggtgtcatt ggtgccgggc aagggcggcg
    11161 cgtattcctc ttccatgcgt tcgagaagag gattttcctg caggtatttt tcgagttcct
    11221 gctcgagttc gagcgtcgac agttgcagca gacgtatcga ctgctgcaac tgaggggtca
    11281 gtgcaagatg ctgcgaaagc ttgagctgga gggatggttt catttacgga gcctgagcgg
    11341 tcgccgtttc gcgggagggc aactcgtttc gttccgggat tttaggtgca agcggcaggg
    11401 tcaaaggcgg aaattctcgc ccagatatac cttgcgaacg ttttcattat aaatgatttc
    11461 atcaggccgg ccagcggcga gaacgctgcc gtcgttgagg atataggcat ggtcacaaat
    11521 gcccagggtt tcgcggacgt tatggtcggt aatcaagacg ccgatgtcgc gctctttgag
    11581 aaagtgaatg attttctgga tatccagcac cgcgatgggg tccacgccgg caaagggctc
    11641 gtcgagaagg atgaagcgcg gctcggtaga cagcgcacga gcaatttcga ctcgtcgacg
    11701 ttcaccgccg gaaagcgaag cggccgaact gctgcgaata tggcttatgt gcagttcttc
    11761 gagaaggctt tcggcgcgga attcaatttc gtcaggcggc agattctgta gttccagaac
    11821 tgcctggatg ttttcgacaa cggtcagttt gcggaataca gaggcttcct ggggaaggta
    11881 agagacgccc aactgcgctc ttttatggat gggcaagtgc gtgatttttc tatcatcaag
    11941 atgaacatcg cccccatcac agggaatcag accgacgacc atgtagaagc aggttgtctt
    12001 tccagcgcca ttcgggccga gcaggccgac cacttcgccg cttcttacct cgaaggacac
    12061 gtcttccacc accgtacgct tcttgaagcg ttttttcagg ttgtgcgtgg aaaggagact
    12121 cttcgtgctc gggttgacat tggtttcgga aatcattcaa tcgtcttcaa agtcgccgcg
    12181 taacactcgg cggattatat ctccgggaaa tccccggctc atcaaaaaac gtgtttgtcg
    12241 ggcgcgcccg gctgcatcgc ttgggccggt tttgttgcca aatttgccta gccagaccag
    12301 acgcgcacgc gtcagttcag ggccgctggc ggccaatgcg ccatccatca gttcctcgga
    12361 aatgccctga gtgcgcagct cgtatgcgag ccgagcattg ccaaatcgcg ccgcacggga
    12421 gttcattcgc atttcgacgt accgctcatc ggatagcagc cgtcgtgcga ccaagtcatc
    12481 cagaagcgcc tcaagctctt cggccgaatc ggcgtgcggc gagagcttcc gtgcaagctc
    12541 ggctcgtgcg tgttcgcggc gcgccagcaa ccgcaaagca cgctcgcgca gtgtgctgct
    12601 catgcccgct cattcagcgg cattgaacgg cttgggcgag ggcacattaa ctgcagcgcg
    12661 gattttggcc tcaatctcct cggcgatttg cgggtttgcc ttgaggtatt cacgagcatt
    12721 gtccttgccc tggccgatct tgtcgccgcc gtaggcgtac caggaaccgg atttttcaac
    12781 cagtttgtga attaccccga gctcgacgat ttcgccttcg cgcgacgttc cttcgccgta
    12841 aaggatgtca aaaatggctt cgcggaaagg cggcgcaacc ttgttcttga caaccttgac
    12901 gcgggtttca ttgccgatca cttcgtcacc cttcttgatg ccgccaatac ggcggatatc
    12961 caggcgcacc gaagcgtaaa acttgagcgc attgcctccg gtcgtggttt ccgggctgcc
    13021 gaacatgacg ccgattttca tgcggatctg gttgatgaag atcaccaggg tattggtccg
    13081 gttgatgttg g
//
LOCUS       DAORPM010000033        12826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1010_length_12826_cov_10.706131, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000033 DAORPM010000000
VERSION     DAORPM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12826
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_12826_cov_10.706131"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLD53_02880"
     CDS             <1..521
                     /locus_tag="PLD53_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRC59880.1"
                     /translation="QEVPSDVVPFEQRPWMKAAEIADAMIEALKSGKYRTLRCNFANG
                     DMVGHTGNFRAATMSIEAVDLELARILPVIDAMGGVALITADHGNADEMYELDKKTKQ
                     PAQNKDGSFKAKTAHTLNPVPLILYDNVSGGKLGLKQTPTAGLSNLAATTANLLGLEK
                     HAKWDESLLEIK"
     gene            complement(660..1091)
                     /locus_tag="PLD53_02885"
     CDS             complement(660..1091)
                     /locus_tag="PLD53_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC59881.1"
                     /translation="MKTLKQLIESKSKKLATVSPKDTVLHALEVMAQYDVGALIVLDG
                     QRLAGMFSERDYARKVILQGKVSVYTKVGDIMTDKVISVTMNHTIEQCLAIMSEKHFR
                     HLPVLDDEGLVVGIISIGDMVKEMISQQQFIINQLESYISS"
     gene            complement(1150..1785)
                     /locus_tag="PLD53_02890"
     CDS             complement(1150..1785)
                     /locus_tag="PLD53_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRC59882.1"
                     /translation="MIRNTALAFFVATSFSVFAAEEATTKLDLAKGKQIAETVCVGCH
                     GADGNSPTSANPSLAGQGSEYLFKQLTQFKSDDGKPAVRNNPIMSGMTAALSVEDMKS
                     LAFYFSQQKLKPATATDEKLVSAGKSLWRKGDFERGIPACAGCHGPTGAGVPAQYPRI
                     AGQYAEYTEAQLKNFRSEERSNDPEMMMRNIAEKLSDKQIKAVADYAAGLR"
     gene            1896..2636
                     /gene="yihA"
                     /locus_tag="PLD53_02895"
     CDS             1896..2636
                     /gene="yihA"
                     /locus_tag="PLD53_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRC59883.1"
                     /translation="MPLFQKAVFHTTVANLPDLPSDSQREVAFAGRSNAGKSSAINTL
                     ANHTRLAFVSKTPGRTQHLNYFSLDEGKYLVDLPGYGFAKAPEEIRSQWESLLAPYLH
                     YRVPLVGLVLIMDSRHPLTELDLQMIEWFALTAKPIHVLLSKADKLTRQEQALALREV
                     RTVLSQIGSNYSLQLFSSLSRMGVDEAESVLGGWLGMEPKHYALPPVGLRTEKVARPK
                     NRKAIGWSARTAKAAVKSVQNKKPPAKG"
     gene            2797..3807
                     /gene="hemB"
                     /locus_tag="PLD53_02900"
     CDS             2797..3807
                     /gene="hemB"
                     /locus_tag="PLD53_02900"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRC59884.1"
                     /translation="MNADLSFPGTRMRRMRRDDFSRRLMRESALTVDDFIYPAFVLEG
                     HRRSEKVTSMPGVERLSLDRLLGTAERACELGIPALALFPVIDPGGKTPGAEESFNPE
                     GLIPRAVAALKKELPELGIITDVALDPYTSHGQDGLIDADDPRGYVLNDETLEVLAKQ
                     ALTHAQAGADVVAPSDMMDGRIGRIRAELDAAKLIHTRILAYSAKYASSFYGPFRDAV
                     GSAGNLSGGNKYTYQMDPANSDEALREVALDLGEGADMVMVKPGMPYLDIVRRVKDEF
                     GAPTYVYQVSGEYAMLKAAALNGWLDEKACVLESLLGFKRAGADGILTYFALDAAAWL
                     ND"
     gene            complement(3814..4695)
                     /locus_tag="PLD53_02905"
     CDS             complement(3814..4695)
                     /locus_tag="PLD53_02905"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HRC59885.1"
                     /translation="MNIPQPGILLPVPNQARYLSFSLGDPSLIRQGLHMLRELVDGEY
                     TVVGLGQTLVSALDATIPGLKSFPMITAPGLAIENAPEVLWIWLRGEERGEILLRSQR
                     LARALAPAFQLKEIWDAFKYAEGRDLSGYEDGTENPTGDEATSAAIVGTGNQTLAGSS
                     FVATQRWLHNFDSLTSMSENEQDNCIGRRLADNEEMDDAPESAHVKRTAQESFTPEAF
                     VLRRSMPWAEGNNGGLMFVAFGATFYAFEAQLQRMVGADDGIVDAIFKFTQPVSGAYF
                     WCPALKNGKLDLTPLAR"
     gene            complement(4715..>5210)
                     /locus_tag="PLD53_02910"
     CDS             complement(4715..>5210)
                     /locus_tag="PLD53_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HRC59886.1"
                     /translation="WEHQALTRARFCAGDAELGAQFEAIRIEILRQPRDLAKLMEDVL
                     AMRKRMLDNHATTSDEYFDIKQDPGGLIDVEFIVQYLVLGHAHAHERLCGNLGNIALL
                     GIAAELGLIPPSLADPVRDTYREFRRIQHALRLNGISGARVERAEHEHRIEAVSALWT
                     FVFD"
     assembly_gap    5211..5310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5311..7245)
                     /gene="glnE"
                     /locus_tag="PLD53_02915"
     CDS             complement(<5311..7245)
                     /gene="glnE"
                     /locus_tag="PLD53_02915"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HRC59887.1"
                     /translation="MMTPAASDSLPPEWHPALAHSRYLRQLLDARPDVVVWLNDFANH
                     ALNGELMRGFLDAEAPSDEGSLKSVLRRLRQRIMAALVVRDIGGKAPLADVVETMTQL
                     ADVTTNFALDFVHRQLAAQFGEPLDSQGNPQRLLIVGMGKLGGRELNVSSDVDYIFVY
                     PEEGETAGSSDGGRRIEAYEFFQRVGKRLIATLGEITGDGQVFRVDMRLRPNGDSGPL
                     VCSLDSLESYFITQGREWERYAWIKARVMNVGNNLQPEWSTALEKVARAFVFRKYLDF
                     GAINAMRDLHAQIRREVVRKDMANHIKLGPGGIREIEFIAQVFQLIRGGRDPALQIRP
                     TLKVLMLLVERRLLPPDGVDELVAAYVFLRCLEHRLQYLNDAQTHMLPASDEERHLIA
                     ASLGFSDWPALLGVLDAHRERVTRHFGEIFSNPDESSHALAGLWFEQTDGDDALEILS
                     ELGFRQSQGLLEQLRSFRRSAKYLQLPTQNRERLDALGPRLIEAAAGTDSPDATLQRG
                     LDFLETISRRGAYLALLQQYPQTLSKVAEIIGSSSWAADYLTRHPILLDEVLDTRLYE
                     IATDWAAFSTELNQRLAEYVGDTEREMDILREVHHAQVFRLLAQDIAGLQTVERLADH
                     LTELADIVVRTTLELCWGKLK"
     gene            7426..>9814
                     /locus_tag="PLD53_02920"
     CDS             7426..>9814
                     /locus_tag="PLD53_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HRC59888.1"
                     /translation="MSLGEGLRSALYHRFHVLVPFVSHRFVRGMWRAVVWSFWLIYFG
                     FIVLVLSLRYGVLPHIENYRVDIERFASQELGQTVSIGRIEASWEGINPDLTLLDVRI
                     ADSDGRPALAFSRVESILSWWSVPSAQLKLRLLRIDEPTLNLRRSADGRVFVAGIPIN
                     RERNDNDVSGWILEQRRIRIHGATLVWEDELRGAPTLVLEDVNFALDNDGKRHRFGLT
                     ALPPDALASKVDVRGDFRVKSIDEIKTWAGQVYAEIDYADLAVWQQWVDYPVALPHGQ
                     GGIRAWLGFAEGGLREIVADVSLQDVSVRLAQDLPVLALDHVSGHFGAKIPETGFEVT
                     GRQVGLSTRKATILNSEPRETIRIEPMDFHLNWQPDPDDNIVIGSAGASRLDLAALAG
                     LAEHFPFDAHTRQLLSDFAPRGLVSGLSAQWKSDAGHLQTYSLKAGFDGLALKAHGDI
                     PGASGLSGVLEANEKVGNATLRSKKMTIDLPGVFPESPIALDMLNAHAKWKINKGILD
                     AELTRADFAGPDAAGTAQGKYQTAGEGPGIVDLDAALVRADARAVWRFIPRTVGESTR
                     FWLRDSLLSGGASEAKLTLKGNLVDFPFLDKSKGQFLVTVKARDVVLDFATGWPLIEG
                     ISGDLRFEGNGMSIQANRGSIMGAQISNTQVRIPDFDAPISTLIIKGNVDGATSDFLK
                     FIEKSPVADRIDHFTDGMRATGKGHLDLDLFIPLDETKLGDSKIDGKYRFTNNELTVD
                     AALPPFKQLNGSLHFSGEDLQLDEINSTFLGGPLKVKGGSQKDGNVLITANGLVDI"
     assembly_gap    9815..9914
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9915..11588
                     /locus_tag="PLD53_02925"
     CDS             <9915..11588
                     /locus_tag="PLD53_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRC59889.1"
                     /translation="GSQKDGNVLITANGLVDIAQLRKQVDSPLLGKLAGKTPYRGEVR
                     INKRNADLLIESTLVGLSSTFPEPFSKGAGESLPLRFEKKLLPGASGSKGTNAESSAR
                     DQLDASLGSVLSMRLIRKKQPGGFAVERGAIAIGRPLQLPDAGVSVGLTAKRLDIDNW
                     RRLLQPAGAATAIEAPAKPSSPPFVDTISVKTEDLILFGRHYNDADLSISPSAAQWKI
                     RLASRQANGDLQWDVAGRGKLTARFKDIAFDTTTETEALKSGEVISELPALDIVANEF
                     SLGIRHFGRLELLASNDGSIWRLNRIQMSNPYGTLAGKGQWKLSDGKDVTQLQFKIDS
                     SDVGKLLERLGYPGTVRAGTAKLGGRLEWIGPPTLLDYATLSGDMDVAASNGQFMKLD
                     PGAAGKLLGLISLQGLSRRISLDFNDVFSQGFAFDSITGKVAVATGLMRTERLQIDGP
                     SARVTMRGEVDLKQETQRLNVNVQPELGGTAALGVALINPIAGAATWLAHKALQNPLN
                     QMFGFDYLITGKWDDPIVEKISGVAPAKDAPRLPPPTNPTGATSESSSK"
     gene            11569..12405
                     /locus_tag="PLD53_02930"
     CDS             11569..12405
                     /locus_tag="PLD53_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRC59890.1"
                     /translation="MNPLQNDSSGALKNGRVAAVQMVSTSRIDENLHAARTLIDESVA
                     QGADLVVLPEYFPIMGMRDGDKVKVREAEGRGPIQDFLAETALRHGVWLIGGSIPLVA
                     DAADKVFNSSVAYNPQGELVARYDKIHLFGFQKGKERYNESASIEAGKQPVAFDTPFG
                     RVGLSICYDMRFPELYRALGVVDLLVIPAAFTETTGRAHWEILLRARAIENQCYVLAS
                     AQGGTHENGRETHGNSMLIDPWGDILDRKPKGQGIVIGEIDHARIAEVRANLPALKHR
                     VM"
     gene            12544..>12826
                     /locus_tag="PLD53_02935"
     CDS             12544..>12826
                     /locus_tag="PLD53_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HRC59891.1"
                     /translation="MTENAQSLLVQAQKTLLTPYGLDVADLSKVFGTIMSHDVDYADL
                     YFQYSRSEAWSLEEGIVKSGSFNIDEGVGVRAISGEKTAFAYSDDISHLA"
BASE COUNT         2610 a         3478 c         3822 g         2716 t
ORIGIN      
        1 atcaggaggt gccaagcgac gtggtgccct tcgaacagcg cccgtggatg aaggcggccg
       61 aaattgccga cgcgatgatt gaggcgctaa agagcgggaa atacagaacc ttgcgctgca
      121 acttcgccaa cggcgacatg gtgggccata cggggaattt ccgggcagct accatgtcga
      181 tcgaggccgt cgatctggag ctcgcacgca ttctgccggt gattgacgcg atgggaggcg
      241 tggcactgat cactgccgat catggcaatg ccgacgagat gtacgaactt gacaagaaga
      301 ccaagcagcc ggcacagaac aaggatggat ccttcaaggc caagaccgca cacaccctga
      361 acccggttcc gctgattctt tacgacaatg tgtcaggcgg caagctcggc ctgaaacaga
      421 ctccgaccgc aggcttgtcc aacctggccg ccacgacagc caatcttcta ggcctggaaa
      481 agcatgccaa atgggacgag agcctgctgg agatcaaata agtacgaaag aggaagtcgg
      541 cataggggag cgacggagac tatccaggca atcccttttc cgacccacgc tttcgcgtcg
      601 ggcaaaacaa gaaagagcag gggccaccga ggcacctgct cttttttatc gattgctgat
      661 cagcttgaaa tgtagctttc cagctggttg ataatgaact gttgctgact gatcatttcc
      721 ttgaccatgt cgccaatcga aatgatgccc acgaccagac cttcatcgtc aaggacaggc
      781 aggtggcgga agtgtttttc cgacatgatt gccaagcatt gctcgatcgt gtgattcatt
      841 gtcaccgaga tcaccttgtc ggtcatgata tcgccgactt tggtgtacac agacaccttg
      901 ccctgcagaa tgactttgcg cgcatagtcg cgctccgaga acatgcccgc cagacgctgt
      961 ccatcgagga cgatcagcgc gccgacgtca tattgagcca ttacttcgag tgcatgcagc
     1021 accgtgtctt tgggagagac tgtggcgagt ttcttgctct tgctctcaat gagttgtttg
     1081 agtgttttca tagagcgtcc ttgttggtga ggttaagaca aggtggtttg aaggtttggt
     1141 cagggctgcc tagcgcagac cggcagcgta gtctgctacc gccttgattt gcttgtctga
     1201 gagtttttca gcaatgttgc gcatcatcat ctcagggtcg tttgatcgtt cttcgctacg
     1261 gaagttcttc aactgggcct cggtatattc tgcatattgt cccgcaatgc gcgggtactg
     1321 cgccggtacg ccggcaccgg tcggtccatg gcaacctgca caggcaggga tacccctctc
     1381 gaagtcgccc ttgcgccaga gactcttgcc agccgagacc aatttttcat ctgtcgccgt
     1441 cgccggtttc agcttctgct gcgagaagta aaacgccagg cttttcatgt cctcaacgct
     1501 cagcgcggcg gtcatgccgg acatgatggg gttgttgcga acagcgggct tgccgtcatc
     1561 ggacttgaac tgggtcagtt gcttgaacag gtactccgag ccctggccag ccaagctggg
     1621 gttggcagac gtcgggctgt tgccatcagc gccatggcag ccgacacata ccgtttcagc
     1681 gatctgtttg cccttcgcca gatcgagttt ggttgtcgct tcttctgctg caaaaacgga
     1741 aaagctggtg gcaacgaaaa acgccagtgc cgtgttgcga atcatgtaca aactcctgtc
     1801 gatgcgtccg agttcagaga ggcaaactcc caaacctgat attctatagc actttcgggc
     1861 ttagtcacgc caaccccaac gtctccggtt tgccaatgcc acttttccag aaggcggttt
     1921 ttcacactac cgtcgccaat ctacccgatt taccctctga ctcacaacgg gaggtcgctt
     1981 ttgccgggcg ctcgaacgcc ggaaagtcgt cggcaatcaa cacgctggcc aatcacacgc
     2041 gacttgcatt cgtttccaaa acgccggggc ggacgcagca cctcaactat ttctcgctcg
     2101 acgagggcaa gtacctcgtt gatttaccag gttacggttt tgccaaagca cctgaggaaa
     2161 tccgctcgca gtgggagagc ttgctcgcac cctacctgca ttatcgcgtg ccgctggtcg
     2221 ggctggtgct gataatggac agtcggcatc cgttgaccga gcttgacctg cagatgatcg
     2281 aatggtttgc gctgacggcg aagccgatac atgtcctgct ctccaaggca gacaaactga
     2341 ctcgacagga acaggcgctg gcgctgcgcg aagtgcggac cgttctgagt cagattggca
     2401 gtaattattc gctccagctc ttttccagcc tgagcaggat gggtgttgac gaggctgagt
     2461 cggttctcgg aggatggctg ggtatggagc ccaagcatta tgctctcccc cctgtcgggt
     2521 tgcgcaccga aaaggtcgcg cgtccgaaga accgcaaggc gatcggctgg agtgcaagaa
     2581 ctgcgaaagc cgcggtcaag tcagtacaaa ataaaaagcc cccggcaaag ggatagccgg
     2641 gggcaaaatg ccttacccag aagcaaggcg cccgctcagg gagttgaagc gggagatggg
     2701 atgagccatc tgtcgactga gagtgagtgc tgcgacgctt agttcccggt caggtgccca
     2761 caaatacgat ttattttgtt ttgatacgag atagccatga atgctgactt gagtttcccc
     2821 ggtacgcgca tgcgccggat gcgccgtgac gatttttccc gccgtctgat gcgcgagtcg
     2881 gcgctgactg tcgacgattt catttaccct gcgtttgttc tcgaaggtca tcggcgctcc
     2941 gagaaggtaa catcgatgcc cggcgtcgaa cgtctgagcc ttgatcgctt gctgggaacg
     3001 gcagagcggg cgtgcgagct cggcattccg gcgctggcgc tcttccccgt gattgatccg
     3061 ggcggtaaaa ccccgggagc ggaagaatcc tttaatccag aaggtcttat tccgcgcgcg
     3121 gtggccgcgc tgaagaagga attgcccgag ctgggcatca ttaccgatgt ggcgcttgat
     3181 ccctatacca gccatggcca ggatgggctg atcgatgccg acgacccgcg tggctatgtg
     3241 ctcaacgacg agacgcttga agttctggcg aagcaggcgc tcacacacgc ccaagccgga
     3301 gccgatgtgg tggcgccgtc cgacatgatg gatggacgca ttggtcgcat ccgtgccgaa
     3361 ctggatgccg cgaagctgat tcacacgcgc attctggcct actcggcgaa gtacgcttca
     3421 agcttctacg gccctttccg tgatgccgtc ggatcggcag gcaatctaag cggggggaac
     3481 aagtacacct accagatgga tccggccaac tccgatgagg ccttgcgcga ggtggcgctc
     3541 gatcttggcg agggtgccga catggtgatg gtcaagcccg gtatgcctta cctcgatatt
     3601 gtccgtcgcg tgaaagacga attcggtgcg ccgacctatg tttatcaggt gagcggggaa
     3661 tacgccatgc tcaaggctgc tgcactgaat ggctggcttg acgagaaagc ttgcgtgctg
     3721 gagagcctgc tgggcttcaa gcgggccggg gcggatggaa tactcactta tttcgcgcta
     3781 gacgccgcag cctggctaaa cgactgatcg gattcagcgc gccagcggcg tcagatcaag
     3841 cttgccgttt ttgagcgccg ggcaccagaa atacgcgccg gagaccggtt gggtgaattt
     3901 gaaaatggcg tcgacaattc cgtcatcggc gccgaccatg cgctggagtt gcgcctcgaa
     3961 ggcgtagaag gtggccccga aagccacaaa catcaggccg ccgttgtttc cttccgccca
     4021 cggcatcgaa cgccgcagta cgaaggcttc aggagtaaag ctttcctggg cggtacgctt
     4081 gacgtgtgca gattcgggcg catcgtccat ttcctcattg tcagccagcc gcctgccgat
     4141 gcaattatcc tgctcgttct ccgacattga agtcaaggaa tcaaagttgt gcagccaccg
     4201 ctgcgtagcg acaaagctcg atccggcgag cgtctgattg ccggtgccga cgattgcagc
     4261 gctggtcgcc tcgtcgccgg tcgggttttc ggtaccgtcc tcgtagccgg aaaggtcgcg
     4321 gccttcagcg tacttgaacg cgtcccagat ttccttgagc tggaaggcgg gcgccagcgc
     4381 cctggcaagt cgttgcgaac ggagcagaat ttcgccgcgc tcttcgcccc gtaaccagat
     4441 ccaaaggacc tccggggcgt tctcaatggc cagacccggc gccgttatca tcggaaagct
     4501 tttcaggcca ggaatcgttg cgtcgagcgc cgaaaccagg gtctgcccga gtccgaccac
     4561 cgtatactca ccatcgacaa gttcgcggag catgtgcagg ccttgcctga tcagcgacgg
     4621 gtcgccgagt gagaaggaaa ggtaacgggc ctgattcggc acaggaagga gaattcctgg
     4681 ttgaggtatg ttcatgcgct agctcacagg ttgtttagtc aaagacgaat gtccacagtg
     4741 cgctgactgc ttcgatacgg tgctcgtgct cggcgcgttc gacgcgagcg ccgctgatgc
     4801 cgttgaggcg gagggcgtgt tgtatgcggc ggaactcacg gtaggtatcg cgcaccggat
     4861 ccgccagcga cggcggaatg aggccgagct cggcggcaat tcccagcagg gcaatgttgc
     4921 cgaggttgcc gcacagacgt tcatgagcat gtgcatgacc gagcacgagg tactgaacaa
     4981 tgaattccac gtcgatcagg ccgcccgggt cctgcttgat gtcgaagtat tcatcgctgg
     5041 tggtcgcatg gttgtcgagc atgcgcttgc gcatcgccag tacgtcttcc atgagcttgg
     5101 cgaggtcgcg cggctggcgc aggatttcga tgcggatcgc ttcgaattga gcccccagtt
     5161 cggcgtcgcc agcacagaag cgtgcacggg tgagtgcctg atgctcccat nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cttgagcttt ccccagcaca gctcgagtgt
     5341 ggttctcacc acgatgtcgg ccagttcggt gagatggtca gcaaggcgct ccacggtttg
     5401 cagccccgct atgtcctgcg ccagcaagcg aaagacctgg gcgtgatgga cttcgcgcag
     5461 gatatccatt tcacgttcgg tatcgccgac gtactcggcg agacgttggt tcagttctgt
     5521 gctgaaagcc gcccagtccg tcgcgatttc gtaaaggcgc gtgtcgagca cttcgtccag
     5581 caggatcggg tgtcgggtaa ggtaatcggc tgcccaggag gaactgccga tgatctcggc
     5641 cactttgctc agcgtttgcg ggtactgttg cagcaaggca agataggctc ctcgtcgact
     5701 gatcgtttcc aggaaatcga gtccgcgctg caaggttgca tcgggcgagt cggtgcccgc
     5761 tgccgcttcg atcaggcgcg ggccaagcgc gtcaaggcgc tcacggtttt gtgtcggtaa
     5821 ttgcaggtat ttggcgctgc ggcgaaagct cctgagttgt tcaagcaggc cttgcgattg
     5881 ccggaaacca agttcggaaa gaatctccag cgcgtcgtcg ccatcggtct gttcgaacca
     5941 cagaccagcc agcgcatggg agctttcatc cgggttggag aatatttcgc cgaaatgcct
     6001 ggtaacgcgc tcgcggtgcg cgtcaagaac gccgagcagg gccggccagt cggaaaatcc
     6061 cagcgaagcc gcaatcaggt ggcgttcttc gtcgcttgct gggagcatgt gagtctgagc
     6121 gtcgttcaaa tattgcaggc gatgctcgag gcaacgcagg aatacgtagg cggcgaccag
     6181 ttcatcaaca ccgtcaggcg gcagcagacg gcgctcgacg agcagcatca gcacttttag
     6241 cgttgggcgt atctgcagag ccgggtcgcg accgccgcgt atcagttgga aaacctgcgc
     6301 gataaactcg atttcccgga tgccgcctgg accgagcttg atatggtttg ccatgtcctt
     6361 gcgcaccacc tcgcgacgaa tctgcgcgtg caggtcgcgc atggcattta tggcgccgaa
     6421 gtcgagatat tttcggaaga cgaaagcgcg cgcgaccttt tccagtgcgg tgctccattc
     6481 cggctgcagg ttgttgccga cgttcatgac gcgcgccttg atccaggcgt agcgttccca
     6541 ctcacgacct tgggtaatga aatagctctc gagcgagtcg agcgaacaga ccagcggccc
     6601 cgagtcgcca ttcgggcgca ggcgcatgtc gacacggaaa acctgcccat cgccggtaat
     6661 ttctccgagc gtggcgatca gacgcttgcc gacgcgttga aaaaattcgt aggcttcgat
     6721 ccggcggccg ccatcggaag atccggcggt ttcaccttcc tccgggtaaa cgaaaatgta
     6781 atcaacgtcg gacgacacgt tgagttcgcg tccgccaagc ttgcccatgc cgacgatcaa
     6841 caggcgctgc ggattcccct ggctgtccag cggctcgccg aactgggcgg cgagctggcg
     6901 atgcacgaaa tccagcgcaa aattggtcgt cacatcggcg agctgcgtca tcgtctcgac
     6961 gacatcggcg agcggcgctt tcccgcctat gtcgcgcacg acgagggccg ccatgatgcg
     7021 ctggcgcaag cgtcgcaaga cgcttttgag gctgccctcg tcgcttggcg cttcggcatc
     7081 cagaaaaccc cgcatcagtt cgccattcag cgcgtggttt gcgaaatcgt tcagccagac
     7141 aacgacgtcc ggccgggcgt cgagaagctg gcgcaaatag cggctgtgcg ccagtgccgg
     7201 atgccattca ggcgggagcg aatcactggc ggcaggcgtc atcatttcgc gcattttttg
     7261 caggaattgc ggtttgtctc cggcgattgc cccgtctgga tagtctgttt ggcgtggctt
     7321 ggccggtcaa tgccgttaca atcgagactt caatactttc atgcatcacg ccattttagt
     7381 gacgcttccc gacaattggc aaagcattcc gcaggtgacc ggttcatgag tctcggtgaa
     7441 gggttgcgtt ccgcgctgta ccaccgcttt catgtgctgg tacctttcgt gtcacaccgg
     7501 tttgtccgtg gtatgtggcg tgccgtggta tggagtttct ggctgatcta cttcggcttt
     7561 atcgttctgg tcttgtcatt gcgctatggc gtcctgcccc atattgaaaa ctatcgcgtc
     7621 gacattgagc gcttcgccag ccaggagctt gggcaaaccg tcagtatcgg ccggatcgaa
     7681 gcgagttggg agggtatcaa cccggatctg accttgctgg atgtgcgcat cgccgattcc
     7741 gacgggcgac cggcgctcgc gttctctcgc gttgagtcga tcttgtcctg gtggtcggtc
     7801 ccgagtgcgc agctcaagtt gcgactcttg cgcattgatg aaccgacctt gaacctgcgt
     7861 cgcagcgccg atggccgggt attcgtcgcc ggaataccca tcaaccgtga acggaatgac
     7921 aacgatgtct ctggctggat tcttgaacag aggcgaattc gcatccacgg tgcgactctg
     7981 gtctgggagg acgagctgcg cggtgcgccc accctggtcc tggaggatgt taacttcgct
     8041 ctcgacaacg acggcaagcg ccatcgtttc ggcctgactg cgctacctcc ggatgcattg
     8101 gcatccaagg tcgatgttcg cggcgatttc cgggtaaagt ctatcgacga gatcaagacg
     8161 tgggcgggtc aggtctatgc cgagatcgac tacgcagacc tggccgtctg gcagcagtgg
     8221 gttgattatc cggtggcgct accgcatggg caggggggca ttcgtgcgtg gttgggcttc
     8281 gccgaaggcg gcttgcgcga gattgtggca gatgtttcgc tgcaggacgt aagcgtgcgt
     8341 ctggcccagg atcttcctgt cctggcgctc gatcatgtgt ccgggcattt cggcgccaaa
     8401 attccagaga ccggctttga ggtcaccggg cgacaggtcg gtttgtcgac acggaaggcg
     8461 acgatactga atagcgagcc ccgcgagaca atacgtatcg agccgatgga ttttcacttg
     8521 aactggcagc ccgacccgga cgacaacatc gtcatcggta gcgccggcgc gagccggctt
     8581 gacctggctg cgctggccgg tcttgccgag cattttcctt ttgatgcgca tacccgccag
     8641 ctattgagtg attttgcccc gcgtggcctt gtcagtggat tgagtgcgca atggaaaagc
     8701 gacgcgggtc atctgcaaac ctattcgttg aaagccgggt tcgacgggtt ggcgctgaag
     8761 gcacacggtg atatcccggg tgcttccggc ttgtcggggg tacttgaggc caatgaaaaa
     8821 gtgggcaatg caacgttgcg ctcgaagaaa atgacgattg atcttccggg tgtttttccg
     8881 gaatcgccca ttgcgcttga tatgctcaat gcgcatgcca agtggaaaat caataagggt
     8941 atccttgatg cggaactgac acgcgccgat ttcgccgggc cggacgccgc tggaacggcg
     9001 cagggaaaat accaaaccgc aggagaggga ccgggtattg tcgatctcga cgccgcactg
     9061 gtccgtgccg acgcgcgcgc cgtctggcga ttcatccccc gtacagtggg cgagagcaca
     9121 cgcttctggt tgcgtgattc cttgttgtcc ggtggcgcca gcgaggcgaa actgaccctc
     9181 aagggcaacc tggttgactt tcctttcctg gacaaaagca aaggccagtt tctggtaacg
     9241 gtcaaagcgc gggacgtagt acttgatttc gccacgggat ggccgctcat cgaaggaatt
     9301 agcggagacc ttcgttttga gggcaacggc atgtccattc aggcgaatcg tggatcgatc
     9361 atgggcgcac aaatctccaa tacgcaagtg cggatccccg attttgatgc gccgatttcc
     9421 accttgatca tcaagggaaa tgttgacggt gccacttccg actttctcaa attcatcgag
     9481 aagagcccgg tcgccgacag gatcgaccat tttaccgatg gcatgcgggc caccggaaag
     9541 gggcatctgg atctggatct gttcattcct ctcgacgaaa cgaaactcgg tgattcgaag
     9601 atcgatggga agtaccgatt cacaaacaat gaattgacgg tcgatgccgc gctgccccca
     9661 ttcaagcaac tgaatggcag tttgcacttt tccggtgaag atctgcagct ggatgagatc
     9721 aattcgacct tccttggcgg accgctcaag gtcaagggag ggtcgcagaa ggacggcaat
     9781 gtactgatca cggccaacgg tctggtcgac atcgnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnngggtcg cagaaggacg gcaatgtact gatcacggcc aacggtctgg
     9961 tcgacatcgc tcagctacgc aaacaggtgg actcaccact actgggtaag ctggccggga
    10021 aaacacctta tcgaggtgag gtgcgcatca acaagcgcaa cgccgatctg ctcatcgagt
    10081 cgacactggt cggtctgtct tcgacttttc ccgagccatt cagcaagggt gctggcgagt
    10141 ctctgccact gcgtttcgag aagaaactcc tgccgggggc ttccggctcc aaggggacga
    10201 atgccgaatc gtcggcgcgt gatcagcttg acgcctcact cggcagcgtt ctttcaatgc
    10261 gcctgattcg caaaaaacag ccgggaggct tcgccgtcga acgtggagcg atcgccatcg
    10321 gtcgccccct gcaattgcct gatgccggtg tttcggtggg actgacagcg aaacgtttgg
    10381 atattgataa ctggcgtcgg ctgcttcagc cagcgggagc agctacggcg attgaggctc
    10441 ccgccaagcc ctcttcgcca cccttcgtgg atacgatcag cgtcaagacg gaagacctca
    10501 ttctttttgg gcggcactac aacgatgccg atttgagtat ttcgccatcc gctgcacaat
    10561 ggaaaattcg tctggcctcg cgtcaagcca acggcgactt gcaatgggat gtggctggaa
    10621 gaggcaagct gacagcacgt ttcaaggaca tcgcgttcga cacgacaaca gagacagaag
    10681 ccctgaagag tggcgaagtg atcagtgaac tgccagcgct tgatatcgtg gccaatgagt
    10741 tctccctggg aatacgccat tttggcaggc tcgaattgct ggccagcaac gacggcagta
    10801 tctggcgact gaaccggatc cagatgagca atccgtatgg cactcttgcg ggcaagggtc
    10861 agtggaagct aagtgatggc aaggatgtga cccagcttca gttcaagatc gacagcagcg
    10921 atgtcggcaa gctgctggaa cgactcggct atccaggtac ggtgcgcgcc ggaacggcga
    10981 aacttggtgg gagactggaa tggatcggtc cgccgacttt gctcgactac gctaccctga
    11041 gtggcgatat ggatgttgcg gcgagcaacg gacagttcat gaagcttgat ccgggggcgg
    11101 ccggaaagct gctcggtttg atcagcctgc aaggactgtc acgccgcatt tcgcttgatt
    11161 tcaatgatgt tttcagccaa ggatttgcct ttgacagtat caccgggaag gtcgcggttg
    11221 cgactggact gatgcgcacg gagcgactgc agatcgacgg gccgtcggcg cgcgtgacga
    11281 tgcgcggcga ggtcgatctc aagcaggaaa cgcaacggct taacgtcaat gttcaacccg
    11341 agctcggcgg caccgccgcg ctgggtgttg cactcatcaa cccgattgcc ggcgcggcga
    11401 cctggttggc gcacaaggcg ctgcagaatc cactcaatca gatgtttggt ttcgattatc
    11461 tgattaccgg caagtgggat gacccgatag ttgaaaagat ttcgggcgtc gcacccgcga
    11521 aggacgcgcc tcgcttgccg ccaccgacca atccgacagg agccaccagt gaatcctctt
    11581 caaaatgatt cttccggggc attgaagaac ggacgggtgg ccgctgtgca aatggtttcg
    11641 acatcgcgca tcgacgagaa tcttcatgct gccaggacat tgattgacga gtcggtagca
    11701 cagggcgccg atctggttgt gctgcccgag tacttcccga tcatgggaat gagagacggc
    11761 gacaaggtca aggtgcggga agccgagggc cgcggtccga tacaggattt tctggccgaa
    11821 actgcgctca ggcacggtgt ctggctgatc ggtggttcga tccccctggt agccgacgca
    11881 gcggacaagg tgttcaattc cagtgtggcc tacaatcccc agggcgagct cgtggcgcgc
    11941 tacgacaaga ttcacttgtt cggctttcag aagggcaaag agcgctacaa cgaaagtgca
    12001 tcaatcgagg cgggcaagca gccggtggct ttcgataccc ctttcgggcg ggtgggcctg
    12061 tcgatctgct atgacatgag gtttcccgag ctgtatcgtg ccctgggtgt cgtggacttg
    12121 ctggtcatcc cggcggcgtt caccgaaacg acggggcgtg cgcattggga gattctgttg
    12181 cgcgcccgtg ccattgaaaa ccagtgctac gtgttggctt cggcacaagg cggcacgcat
    12241 gagaatgggc gggaaacaca cggcaacagc atgctgattg atccctgggg cgatattctc
    12301 gaccgcaaac cgaaagggca gggtatcgtg attggagaga tcgaccatgc gcgtatcgcg
    12361 gaagtccgtg ccaacctgcc ggcattgaaa catcgggtga tgtgatgttg gtcagtattc
    12421 accaccaagt caccaagaca cgaagagtca ccaagtaaag cacggatttc ttcgtgtgac
    12481 ttcgtgatcc tgctgtcttg gtggtaagtc ttttgaattt gtcttgtcta gatcggaaaa
    12541 ccaatgacag aaaacgcgca atcactgctc gtgcaggcac agaaaaccct gctgactcct
    12601 tatggtctgg atgtggctga tctgagcaag gtgtttggaa cgatcatgtc gcatgacgtc
    12661 gattacgccg atctgtactt ccaatacagc cgctctgaag cgtggagtct ggaagaaggg
    12721 atcgtcaaat cgggaagctt caatatcgat gaaggggttg gtgtgcgcgc catctcgggc
    12781 gagaagacgg cgtttgccta ctccgacgat atcagccacc ttgcgc
//
LOCUS       DAORPM010000034        12760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1018_length_12760_cov_10.125384, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000034 DAORPM010000000
VERSION     DAORPM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12760
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1018_length_12760_cov_10.125384"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLD53_02940"
     CDS             complement(<1..743)
                     /locus_tag="PLD53_02940"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRC59892.1"
                     /translation="MVYPPSASMETRIGNRSPIVPASVSRDIRSKLPLERLPMMPQVL
                     VRLLDLCHRDNVNFSDIAVVIHRDAAMTAKVIAVASSASQYGRSRPTNLDQCLTMLGM
                     STIKTIVINESVLQVFRRFTMDRDFDLRQFWGHSLRCALLARELATIMDYANKEEAYL
                     GGLMHDVGQLAILAADANAYFPLLHTYGDNDELCQREQETFDLTHAEIGAWLVEKWEF
                     DSFLSDAVLYHHDPAERIVAAHKLVRIIYL"
     gene            complement(1077..1934)
                     /gene="ispE"
                     /locus_tag="PLD53_02945"
     CDS             complement(1077..1934)
                     /gene="ispE"
                     /locus_tag="PLD53_02945"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRC59893.1"
                     /translation="MSNRAQWTWESIFPAPAKLNLFLHVTGRRDDGYHLLQTLFRFVD
                     YGDSLRFAPRNDGNVVLTTPLPGVPPEGDLTVRAARQLLAETGCRKGVDISIEKRLPM
                     GGGLGGGSSDAATVLLALNHLWQLGLSRQRLQRIGLTLGADVPAFVFGRNAFAEGVGE
                     MLQPMDVPPAWYVVLEPPVQVQTAVIFSAPELKRSTTPIRMVDWKPGVGVNDLEPVVT
                     FHFPAVAEYLRWLSAYGRARMTGSGACVFAEFGQQEAASSAIAGLPSGMRGWVAAGLD
                     CHPLHDLAA"
     gene            complement(1931..2521)
                     /gene="lolB"
                     /locus_tag="PLD53_02950"
     CDS             complement(1931..2521)
                     /gene="lolB"
                     /locus_tag="PLD53_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HRC59894.1"
                     /translation="MSTYRTAGLLLCAMFLVSCAELPSSPDRASPKRSELLAFSLEGR
                     FSLRHEDTNYSGSLRWRHDGANNELLLVSPFGQGIAEIVTSDSGARLTTSDGKVYSEA
                     NAETLTRQVLGYTLPLARLTDWVRAQGVADGVAERDDQGRPLRMRHEDWKVDYGYASD
                     DPDALPDRIFAERTGGFELRLRIDEWGSLQPTDRKP"
     gene            complement(2574..4316)
                     /locus_tag="PLD53_02955"
     CDS             complement(2574..4316)
                     /locus_tag="PLD53_02955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC59895.1"
                     /translation="MKHFRITLCLAVLPVFLSLPALAADKAAPATKKAPAVREAKRPV
                     PSAPRTSDAESEGRAVYQVLLAEIALQRGDLDLASKAYADLALRTRDPKVLERTIEVA
                     GHARRFDLALEAARLWLDVEPSSKRAQQMMVSVMILSNQLAGLAPSLVRLLEADKASL
                     GENLLGLNRMFARNPDRKAVFRLIDEVCTPFFGIAEAHYAVALSAGSADEFERALVEV
                     RRALELRPDWEMAALLEAQLQTRESPAAGIAFMQGFVDSNPKASDMRLSLARALVGEK
                     RYAEAKHHFDQLLLEYPDKPEIVYPAAILALQQNEIALAESRFKHLVTLDIPDKSLAY
                     YYLGQIAEEGKQTAEALGYYAMVGAGEHNVPAHVRSARLLAQQGKLELARAQLRSAKG
                     ATPEERIQLQIAEAGLLREAKQTQAAFDLLDEALTAQPEQPDLLYETALLAEKLDLME
                     VMESRLRKLIELRPDSAQAYNALGYSYAERNIRLIEALALISKALRIAPNDPFILDSM
                     GWVLYRQGDLPGALTYLEQAYALRDDPEVAAHMGEVLWTLGRVEDAQRTLHEAQKKHP
                     TNEALTAAVKKFAP"
     gene            4412..5239
                     /gene="mutM"
                     /locus_tag="PLD53_02960"
     CDS             4412..5239
                     /gene="mutM"
                     /locus_tag="PLD53_02960"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRC59896.1"
                     /translation="MPELPEVEVSRQGLLPYLPGQRILDAIFRTQKLRHAIPAGLASR
                     LTGLRVDGIHRRGKYLLFDCESKLDGGWLILHLGMSGSLRLVTPVMPAEKHDHVDLVF
                     ADTVLRFRDPRRFGALIWHEGSHVDRHPLIAVLGVEPLSTAFDGDWLYAQTRRRRAPI
                     KPLLMDSHLVVGIGNIYASESLYRAGISPLRAANRIRQERYRILVASIRSTLETAIGA
                     GGSSVRDYVHSDGGMGCFQLSCAVYNRQGDPCPTCGRPIRLVRQAGRSTFYCQYCQR"
     gene            5360..>7140
                     /locus_tag="PLD53_02965"
     CDS             5360..>7140
                     /locus_tag="PLD53_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HRC59897.1"
                     /translation="MTLAQQFAAYSEWRTQLSQGIEAFRRCLSENELSDAQTELRIGQ
                     LLETLREDRLHVAFVAEFSRGKSELINAIFFANYGNRMLPSTAGRTTMCPTELMFDAG
                     KPPSIELLPIQTREGNASISEYKRFPDEWRIVPLDIESGESVQEALRSVSEIERVSPQ
                     AAEKFGFTSGDNQSGVVRLAEDGMVEIPRWRHAIINFPHPLLQQGLVILDTPGLNAIG
                     TEPELTLSLLPNAHAVIFILAADTGVTQSDLAVWNEHIGAPSGRKKGRIVVLNKIDSM
                     WDELKTEEEIDAEITKQTNSCAWTLDLPSNQIFPISAQKALVAKINNDTELLERSRLP
                     ELESALSKELIPAKQEIIHENTDAEFADIYMRMRGLLESRLSGLREQLGELNELRGKN
                     KGVVEYMMGKVRVEKEEFESGLQRYYAVRSVFSQLTNKLFGHLGLDALLDLTTSTREK
                     MIEAPFSKTLSGAMKNFFNVSRNNLVKSDGEIVEILTMMGAIYKKFAVEHGLKLGTPT
                     SFSLIRYQKDIDRLEYWCDTHLNTMFQLMTHEKNKVTQKFFEEVATQVKRAFERANRD
                     AESWLKAIMAPMETQVREHQIQLKRRL"
     assembly_gap    7141..7240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7241..7365)
                     /locus_tag="PLD53_02970"
     CDS             complement(<7241..7365)
                     /locus_tag="PLD53_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRC59898.1"
                     /translation="MALIITDECINCDVCEPECPNEAISQGAEIYEIDPGKCTECV"
     gene            complement(7386..7883)
                     /gene="coaD"
                     /locus_tag="PLD53_02975"
     CDS             complement(7386..7883)
                     /gene="coaD"
                     /locus_tag="PLD53_02975"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRC59899.1"
                     /translation="MALNKRAAIYAGTFDPITRGHEDLVRRAACLFDHVIVGIAESRS
                     KRPFFSLAERVEIAKELLAQYPNVEVCGFDGLLMDFLHAKGAKVILRGLRAASDFEYE
                     FQMAGMNRNLFAEVETIFLTPGEKYMFISATMVREIAILGGDVSKFVHPAICERLVKR
                     VAEKF"
     gene            complement(7873..8436)
                     /gene="rsmD"
                     /locus_tag="PLD53_02980"
     CDS             complement(7873..8436)
                     /gene="rsmD"
                     /locus_tag="PLD53_02980"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRC59900.1"
                     /translation="MNTIRIIGGKWRRRILRFPLSAGLRPTPDRVRETLFNWLGQDLT
                     GLICLDLFAGSGALGFEAASRGASKVVMVENAPRTLEALEANARLLAAVAGQLEIVYK
                     DAVRFTSSLRNTGSRFDVLFLDPPFEQGWIDRLTPLLPEILTEDGVLYVEAEHVVHAC
                     GDWRTVRSGRAGQVFYHLMRRGESNGA"
     gene            complement(8456..9757)
                     /locus_tag="PLD53_02985"
     CDS             complement(8456..9757)
                     /locus_tag="PLD53_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRC59901.1"
                     /translation="MKLFQSLCALCLCLSAQIAHAGPKIEHWLAPSGARVFFVENHSL
                     PILDVRVDFAAGAAYDPPGKAGLAALTQALLDMGVQGMDETQISNRMADLGALLSGDV
                     ELDRASVSLRTLSTDDKRIAALDIFRAVLASPQFPPEVFEREQARTVVALKEALTRPD
                     AIAGKAFWAAMYPAYAYGRQASPETVSSLERSDLVSFYSHNYTAQQAAVSIVGDLTRA
                     QAEALAQQLTSSLPSGAGVAALGVPQLPAASEQRIAHPAAQAHLLIGLPALKRGDPDY
                     FPLIVGNYSLGGGGFVSRLMKEVREKRGLAYSVQSYFQPLLQIGPFQIGLQTKKSQAN
                     DALKVTRDVLAGFLAEGPSATELQAAKQNLVGSFPLRLDSNRKILEHVAVIGFYGLPL
                     DYLDHYAENVEKVTVAEVKAAFARHVKQENLVTVVVAGPQD"
     gene            complement(9769..11139)
                     /locus_tag="PLD53_02990"
     CDS             complement(9769..11139)
                     /locus_tag="PLD53_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRC59902.1"
                     /translation="MLRRYARLIPLLLALILVVPADALANPFELQLANGLRVIVKEDH
                     RAPTAAHMVWYHAGSMDETNGTTGVAHLLEHMMFKGTPTTGAGEFSRLVAAEGGRDNA
                     FTSKDYTAYFQQVPKQKLAQMMQLEADRMRHLNLDPKEFAQESRVVMEERRLRTDDQP
                     QSLLFEQMNAVALQAHPYRVPVIGWMNDLENMTVGDARDWYERWYVPNNAIVVIVGDV
                     DHMEVFRLAEKYYGSLPPRALPARKPQDEPEQTGIRRLTVKAPAELPVVLMAYKVPVI
                     RDVENDIDPYALEMLAAILDGHDAARFSKSLIREQRLATSAGAGYDSTSRGPGMFYLM
                     GSPSEGRTRTELETGLRAEIARIAKDGVAAAELARAKAQLVAGVIYKLDSMFAQAMEI
                     GQLESVGISYRLKKRMIEKLQAVSAEQVQAVAQEYFRDERLTVAELDPQPLSQTPRVH
                     SVAPRH"
     gene            11244..12218
                     /gene="ftsY"
                     /locus_tag="PLD53_02995"
     CDS             11244..12218
                     /gene="ftsY"
                     /locus_tag="PLD53_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRC59903.1"
                     /translation="MFGFLKKSADPQNDAQVSTTPEPTWRERLKTGLARTRAQFGGKL
                     KSIFSRGKIDDELLEELETLLLTCDVGMEATQHLLNELRIRAKKLKVDTPEGIQQALS
                     AAMHDLLLPLEKPLQISSQRPFIIMIAGVNGAGKTTSIGKLAKYFHAQGLSVLLAAGD
                     TFRAAAREQLQTWGERNNVTVISQESGDPAAVIFDAISAARARGIDIVLADTAGRLPT
                     QLHLMEEIAKVRRVIQKADPSGPHETLLVLDANFGQNALQQVIAFDKSIKVTGLIVTK
                     LDGTAKAGVVAAIARQCPKPIRFIGVGEGIDDLRPFVARDFVDGLFEQ"
     gene            12307..>12760
                     /locus_tag="PLD53_03000"
     CDS             12307..>12760
                     /locus_tag="PLD53_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC59904.1"
                     /translation="MIACSAVSKRYPQGLEALKEVSFEISAGEMVYLSGHSGAGKSTL
                     FRLLAAIERPTSGSIVVNGQNLAALRRSAIPYLRRNIGLIFQDHKLLFDRSALNNVLL
                     PLAIVGLSAKDASRRAYAALDKVGLHGRENAYPIALSGGEQQRLAIARA"
BASE COUNT         2730 a         3870 c         3503 g         2557 t
ORIGIN      
        1 agataaatga tgcgtaccaa tttatgggct gctacgatcc gctccgccgg atcatggtgg
       61 tagagcacgg catcggaaag gaaggagtca aactcccact tctcaaccag ccaggccccg
      121 atctcggcat gcgtcagatc gaaggtttcc tgttcccgtt ggcacaactc gtcattgtcg
      181 ccataggtgt gaagcagtgg aaagtaggca ttcgcatcag ccgccagaat ggccaactgg
      241 ccgacatcgt gcatcaaccc gccgagatac gcctcttcct tgttggcgta atccatgatc
      301 gtcgccagtt cgcgcgcgag tagtgcgcaa cgtagcgaat gcccccagaa ctggcgcaga
      361 tcgaagtccc ggtccatcgt aaagcgccga aagacctgaa ggacggactc gttgatgaca
      421 atcgtcttga tcgtgctcat gcccagcatg gtcaggcact gatcgaggtt ggtcggccgg
      481 ctcctgccgt actggctggc cgaggatgcg acggcgatga cctttgcagt catcgccgcg
      541 tcacggtgaa tgacgacggc aatatcgctg aaatttacat tgtcacgatg gcataggtcg
      601 agcagtcgca ccaagacctg cggcatcatc ggaagccgtt ccagaggcag cttgctgcga
      661 atgtcccgac tcacgctcgc cggcacaatc ggtgaacgat tcccgatgcg ggtttccatg
      721 ctggccgatg gggggtaaac cattcttgcc tgactccatg caaacgctgt tcaagacacg
      781 cctgaatcga actgcctgcg gcgcggcggg catcctcttg acaatagccg aaaagtaccc
      841 gacctgtcag catgacaggc atacgttgca ggcgcgttac actctgccga gccggatagt
      901 gtttcaaaaa tacaacaagg atcgggaatt atcgtactgc ctcgccggga cgaggcgtcg
      961 cgggagatgc gtcggccaga gattggtcgc gccacatcgc ctccaggctt tcagcatggc
     1021 cacctcccga aatagtcccc ggcaagaaca cggcgcatgg caaatggctg tcacgctcaa
     1081 gccgcaaggt cgtgaagtgg atggcaatcc agacccgcgg ccacccagcc acgcattccg
     1141 gaaggtagcc cggcgatggc gctcgaagct gcttcctgtt gcccaaactc ggcaaaaacg
     1201 caggcacctg aaccggtcat ccgtgcccgg ccgtaagcgg aaagccagcg aagatactcg
     1261 gcaactgcgg gaaaatggaa ggtgacgaca ggctccagat cgttcacccc aacaccaggt
     1321 ttccagtcaa ccatcctaat cggcgtcgtg ctgcgcttga gttcgggcgc gctgaatatc
     1381 accgcggtct gcacttgaac aggcggttcc agaacgacat accaggccgg cggcacatcc
     1441 atcggctgca gcatttcacc cacaccttcg gcaaacgcgt tgcgcccgaa aacaaaggcc
     1501 ggcacatctg ccccaagcgt caggcctatc ctctgcaagc gctgacgaga caaaccaagc
     1561 tgccagagat ggttgagagc gagcagcacg gtcgccgcgt ccgagctgcc gccacccagg
     1621 cctccgccca ttggcaaacg cttttcgata ctgatgtcaa cgcccttgcg acacccggtt
     1681 tcggccaaca actgccgcgc tgcacgaaca gtcaagtcac cttcgggggg cacgccgggc
     1741 aatggtgttg tcaagaccac gttgccatca ttgcgtggcg caaagcgcaa actatcgccg
     1801 tagtcgacaa agcggaacaa ggtctgcaac agatggtagc cgtcgtcgcg ccgtccggtg
     1861 acgtgcagga acagattgag cttggcgggc gctggaaaaa tgctttccca ggtccactgg
     1921 gccctgttac tcacggtttc ctgtccgtgg gctgtaggct gccccactcg tcgattctca
     1981 ggcgcaattc gaatcctccc gttcgctcgg cgaaaatgcg atcgggcaag gcatccggat
     2041 catcgctggc gtaaccgtag tcgactttcc aatcctcatg acgcatgcgt agcgggcggc
     2101 cttgatcatc ccgctcggca acgccgtccg caacgccttg cgcacgcacc cagtccgtga
     2161 gtcttgcgag tggcagggtg taaccgagca cctgcctcgt cagcgtttca gcattggctt
     2221 ccgagtacac cttcccatcg ctcgttgtca gccgcgcgcc gctgtcgctg gtgacgattt
     2281 cagcgatacc ctgaccaaac ggcgaaacaa gaagcagttc attgttcgcg ccgtcatgcc
     2341 gccaacgcag acttccggag tagttcgtgt cttcatgacg caaagaaaac ctcccttcga
     2401 gcgagaaggc gagcagctcg cttctctttg gcgatgcacg atcaggcgat gagggtagct
     2461 cggcacagct cacaaggaac attgcgcaaa gaagcagtcc ggcggtgcga taggtagaca
     2521 cgaagcgtgc gcttgatcga ctcttgacgg ggcttacgcc gataccatcc ccgtcaggga
     2581 gcgaacttct tgaccgcagc ggtcagcgcc tcattggtcg gatgcttctt ttgcgcctcg
     2641 tgcagcgtac gctgtgcatc ctcaacacgc cccagcgtcc aaagcacctc tcccatgtga
     2701 gccgcaacct ccggatcatc ccgcagggca taggcttgct caaggtaagt caaggcaccg
     2761 ggcaaatcgc cttgccggta aagcacccaa cccatgctat cgagaatgaa cggatcattc
     2821 ggtgcaatcc tgagagcttt cgaaatcaat gcaagagcct caatcaggcg aatattgcgc
     2881 tctgcatagg aatagccaag cgcgttgtag gcctgggcac tgtccgggcg caattcgatc
     2941 agcttgcgca gacggctttc catgacttcc atcaggtcga gtttttcggc aagcaacgct
     3001 gtttcataga gcaaatcagg ctgctccggc tgggcggtca gggcctcatc cagcaagtca
     3061 aacgctgcct gcgtctgttt tgcttcgcgc agcaacccgg cttcagctat ctgcagttgg
     3121 attcgctctt ctggcgtcgc gcctttcgcc gatctgagtt gcgcccgtgc aagctcaagc
     3181 ttgccctgtt gcgccagcag gcgcgcgcta cgcacatgcg ccgggacgtt atgctccccg
     3241 gcaccgacca ttgcataata gcccagcgct tccgcggtct gttttccttc ttcggcgatc
     3301 tgtcccaggt aatagtaggc caagctcttg tcaggtatgt ccagcgtcac cagatgcttg
     3361 aatcgcgact cggcaagcgc tatctcgttc tgttgcagag ccagtatggc cgctggatag
     3421 acaatttccg gcttgtctgg atactccaga agcaactgat caaagtggtg ttttgcttca
     3481 gcataccgct tctcgccaac cagcgcgcgc gccagactaa ggcgcatgtc actcgctttc
     3541 gggttgctgt caacaaaccc ttgcatgaat gcaattcctg cggccggaga ctcgcgcgtc
     3601 tggagttgcg cttcaagcaa tgccgccatt tcccaatccg ggcgcagctc gagtgcccga
     3661 cgaacctcaa ccagtgcgcg ctcgaactca tctgccgaac ccgctgacag ggccacggca
     3721 tagtgcgctt cagcgatgcc gaaaaaggga gtgcatacct catcaatcag ccgaaaaacg
     3781 gccttgcggt ccggattgcg ggcaaacatg cggttcagcc caagtagatt ttccccaagc
     3841 gaagccttgt cagcctcaag caggcgcacc aggcttggcg cgaggccagc cagttgattc
     3901 gagagaatca tcacgctgac catcatttgc tgcgcgcgtt tcgatgacgg ttctacatca
     3961 agccacaagc gggcggcttc gagcgcaaga tcgaaacgac gcgcatgtcc ggccacttcg
     4021 atggttcgct caaggacttt gggatcgcgg gtgcgcaagg cgagatcggc gtaggccttg
     4081 ctggccaaat cgagatcgcc gcgttgcagc gcgatctcgg caagcaatac ttgataaacg
     4141 gcccgacctt cgctctctgc atcactcgtt cgaggagcag aaggcaccgg gcgtttggct
     4201 tcgcgaactg ctggcgcttt ctttgtggcg ggtgctgctt tgtctgctgc cagtgccgga
     4261 agactcaaga agacaggcag tacggcaaga cagagggtga ttcgaaagtg tttcatggat
     4321 tccttgcaat tcgcgatcag atcagggctg gttggagtcg tttgcgtcat aatggtttta
     4381 cacatggtat ggatttttat cggtagcagc aatgccagaa ctccccgaag tcgaagtcag
     4441 ccgccagggc ctattgccgt atctgccggg gcaacgtatc ctcgacgcca tttttcgaac
     4501 gcaaaaactg cgccacgcca tcccggccgg actggcttcg cgactcaccg gtctacgtgt
     4561 tgacgggatt caccgacgcg gaaagtattt gcttttcgac tgcgaaagca agctggacgg
     4621 cggctggctg attctgcacc tgggcatgtc cggcagcctt cggctggtaa cgcccgttat
     4681 gcctgcagaa aagcacgacc acgtggacct ggtttttgcc gacaccgtcc tgcgtttccg
     4741 cgatccgaga cgtttcggcg ctctgatctg gcatgaaggc agccacgtcg atcgacatcc
     4801 gctgatcgcc gttctcggcg tggagccgct ctccactgcc tttgacgggg actggctcta
     4861 cgcgcaaacg cgccgacgcc gtgcgccaat caaacccctg ttgatggaca gccatctggt
     4921 ggtcggcatc ggcaacatct acgcatcgga aagcctctat cgggctggca tatcgccctt
     4981 gcgcgccgcc aaccggatcc gccaggagcg ctaccgcatt cttgtcgcgt ccatccgctc
     5041 gacgctcgaa acggcaattg gcgctggcgg cagcagcgtc cgcgactacg tgcatagcga
     5101 tggcggcatg ggttgtttcc agctgtcatg cgccgtatac aaccgtcagg gggatccttg
     5161 tccaacctgc ggcaggccaa ttcgcctggt gcgtcaggct ggtcgatcca ctttctactg
     5221 ccagtactgt cagcgataag gccgttctga tccgtaaaca tcctgctcga acccatcgtc
     5281 gggatgccag ttccatgtgc ttgattgggc gagactttgt gttagagtta gcgaatgctt
     5341 tttcccaatg gaattaatca tgactcttgc ccaacagttc gccgcctaca gtgaatggcg
     5401 cacacaactc tcccaaggca ttgaggcgtt ccgcagatgt ttgtcggaaa acgagctgag
     5461 cgacgcacag accgaactgc gtattggtca attgctggaa acattgcgtg aagatcgact
     5521 gcacgtcgcg ttcgtcgccg agttctcgcg tggcaagtcg gagctgatca acgccatatt
     5581 tttcgccaac tatggcaacc gcatgctgcc atcaacggcg ggccggacaa cgatgtgccc
     5641 gaccgagctg atgttcgatg caggcaagcc gccctcgatc gaactgctcc cgatccagac
     5701 tcgcgaaggc aacgccagca tcagcgaata caagcgcttc cccgatgaat ggagaatcgt
     5761 tcctcttgac atcgaatcag gcgaatccgt gcaggaggcc ctgcggtcgg tcagtgagat
     5821 tgagcgtgtt tccccgcaag ctgccgagaa gttcggcttc acatccggcg acaatcagtc
     5881 cggtgtggtt cgcctggctg aagacggcat ggtcgaaatt ccacgctggc gccatgccat
     5941 catcaacttc ccgcacccgc tgctacaaca ggggctggtc attcttgata cgcctgggct
     6001 gaacgccatc ggtaccgaac ccgagctgac gctgtcgctt cttcccaatg cacatgccgt
     6061 gatcttcatt ctggccgctg acacgggagt tacgcagtcg gatctggcgg tgtggaacga
     6121 gcacatcggc gcgcccagcg ggcgcaagaa aggacgtatc gtcgttctca acaaaatcga
     6181 cagcatgtgg gacgaactca agaccgagga agagatcgac gccgaaatca ccaagcagac
     6241 caatagctgc gcgtggactc tcgatctgcc atcgaaccag attttcccga tttcggcgca
     6301 gaaggctctg gtggccaaga tcaacaacga cacagagttg ctagagcgaa gccgcctgcc
     6361 cgagctcgag tccgccctgt ccaaggaact gatacccgcc aagcaggaaa tcattcatga
     6421 gaataccgat gccgagtttg ccgacatcta tatgcgcatg cgcggccttc tcgaatcgcg
     6481 cctcagcgga ctgcgtgaac aactcggcga actgaacgaa ctgcgcggca agaacaaggg
     6541 cgtcgttgaa tacatgatgg gcaaggtgcg tgtcgagaaa gaagagttcg aatcggggtt
     6601 gcaacgctac tacgcagtgc gcagcgtctt ttcgcaactg acgaacaagc tttttggcca
     6661 tctgggcctt gacgccctgc tcgacctgac gacttcgaca cgcgagaaaa tgatcgaagc
     6721 gccattctca aaaacgctct ctggcgcgat gaagaatttc ttcaacgttt cgcgcaacaa
     6781 cctcgtcaaa tcagacggcg agatagtcga aatcctcacc atgatgggcg caatctacaa
     6841 gaagtttgcg gtcgagcatg gcctcaagct gggcaccccg accagcttct ctttgatccg
     6901 ttaccagaag gacattgacc gccttgagta ctggtgcgat acacacctca acaccatgtt
     6961 ccagttgatg acccatgaga aaaacaaggt cacacagaag ttcttcgagg aagtcgctac
     7021 ccaggtgaag cgcgctttcg agcgcgccaa ccgtgacgca gaatcctggc tgaaagcgat
     7081 catggcgccc atggaaaccc aggttcgtga acatcagatc cagctcaaac ggcggctgga
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acgcattcgg tacacttgcc
     7261 cgggtcgatt tcatagatct cggcgccctg ggagatcgcc tcgttcgggc actcgggttc
     7321 acatacgtcg cagttgatgc attcgtcagt aataatcaat gccatgatgc tgttccttca
     7381 aatcattaga atttttcggc aactcttttc actagccgct cacagattgc cggatggaca
     7441 aacttgctga catccccacc gagaatggca atctcgcgga ccattgtcgc cgaaatgaac
     7501 atgtacttct cgccgggtgt caggaatatc gtttcgacct cagcgaacag attcctgttc
     7561 atccccgcca tctgaaattc gtactcgaaa tccgacgcgg cacgcagacc acgcaatatg
     7621 accttcgcgc ctttggcgtg aagaaaatcc atcagcaggc catcaaatcc gcagacttcg
     7681 acattcgggt actgggccag aagttccttc gcgatttcga cccgctcggc aagcgaaaag
     7741 aagggcctct tcgagcgact ctcggcgatg ccgacaatta catgatcgaa aagacaggca
     7801 gcacgccgaa cgaggtcttc gtgaccccgc gttatcggat caaaggtacc tgcatagatt
     7861 gccgctcgtt tgttaagcgc catttgactc gcctcgtcgc atcaagtgat aaaaaacctg
     7921 ccctgcccga ccactgcgca ccgtacgcca gtcgccgcaa gcgtgaacca catgttcggc
     7981 ttcaacgtaa agcacgccgt cttcggtcag aatctccggc aagagtggcg tcagccggtc
     8041 tatccaaccc tgctcgaaag gagggtcgag aaacagcaca tcaaaacgcg accctgtgtt
     8101 acgaagtgaa gaggtgaatc ttaccgcatc cttatataca atctccaact gtcccgcaac
     8161 cgcagccagc aggcgagcgt tcgcttcgag cgcctccagg gtccgcggcg cgttctcgac
     8221 catcactact tttgacgcgc cacgagaggc cgcttcaaat cccagcgcac cgcttccggc
     8281 aaaaagatcc aggcagatca gccccgtcag atcctgcccc agccagttga acaaggtttc
     8341 gcgcacgcga tcgggcgtcg gtcgcaaacc agcagaaagc ggaaatcgca aaatgcgccg
     8401 ccgccatttg ccgccgatga tgcgtatggt attcaatgat tgcggcaggc gtcttctagt
     8461 cctgcggtcc agccacgaca accgtaacca gattttcctg cttcacatgg cgtgcaaacg
     8521 ccgccttgac ttcggctacc gtaaccttct cgacgttctc ggcatagtga tcgagatagt
     8581 cgagcggcaa accgtagaaa ccgatcaccg ccacatgttc aagaatcttg cgattgctat
     8641 ccagacgcaa cgggaagctg ccaaccagat tctgcttggc cgcctgcaat tcggtcgcgc
     8701 tcggcccttc agcgagaaag ccggccaaca catcacgcgt caccttcagg gcatcgttgg
     8761 cctgcgactt tttcgtctgc agtccaatct ggaacggccc gatttgcagc aatggttgaa
     8821 aatagctctg cacgctgtaa gcaagtccac gtttctcgcg cacttccttc atcagccgcg
     8881 aaacaaaacc gccgccgccg agagaatagt tgccgacgat caatggaaag tagtcgggat
     8941 cgccgcgctt cagcgcgggc aaacctatca gcagatgcgc ctgcgctgcc ggatgcgcga
     9001 ttcgttgttc agatgcggca ggcaactgcg gcacccccag cgccgcgacg cctgcacccg
     9061 acggcagtga ggatgtaagc tgctgcgcca gcgcttcagc ctgtgcccgg gtgaggtcgc
     9121 cgacaatcga cactgccgcc tgctgcgccg tgtagttgtg gctatagaac gagaccaggt
     9181 cggagcgctc gagcgaactc accgtctcgg gtgacgcctg ccgcccgtaa gcatacgccg
     9241 ggtacatcgc cgcccagaac gccttgccgg caattgcatc ggggcgcgtc aacgcttcct
     9301 tcagtgcaac gactgtacgc gcctgctcac gctcgaacac ttcaggcggg aattgcgggc
     9361 tggccagtac ggctcggaat atatccagag cggcgatacg cttgtcgtca gtcgaaagtg
     9421 tgcgcaaact gacagatgcc cgatccagtt ccacatcgcc ggaaaggagg gcgcccaggt
     9481 cggccatgcg gttggatatc tgcgtctcat ccataccctg gacacccata tcaagcaatg
     9541 cctgggtcag cgcagcgagt ccggccttgc ccggtgggtc gtaagctgca cccgctgcaa
     9601 aatcgacccg cacatcgaga atcggcaaac tgtgattctc aacaaagaag acacgtgcgc
     9661 cggagggtgc cagccaatgc tcgatcttcg ggccggcatg ggcaatctgg gcggacaggc
     9721 acaggcacag tgcgcaaagt gattgaaata gtttcattgt caattctctc aatgacgggg
     9781 tgcgacagag tgcacacgtg gcgtctgcga caggggttga gggtcgagtt cggcgaccgt
     9841 cagacgctca tcacggaaat actcttgtgc caccgcctgg acctgctctg cgctcacggc
     9901 ttgcagcttt tcgatcattc ttttcttcag ccgataggaa atcccgaccg attcgagctg
     9961 cccgatttcc atggcctgcg caaacattga atcgagcttg tagatcacgc cggcaaccag
    10021 ttgcgccttg gcccgcgcca actcggcggc ggcaacgccg tctttggcaa tgcgcgcaat
    10081 ctcggcacgc aaacccgtct ccagctcggt gcgggttcta ccctcgctgg gggaacccat
    10141 cagatagaac atgccagggc cgcgagaagt cgaatcgtaa ccggcccccg cagacgtcgc
    10201 caggcgctgt tcgcggatca ggctctttga aaagcgggcc gcatcgtgcc cgtccagaat
    10261 tgccgcaagc atttcgaggg catagggatc gatatcgttc tcgacatcgc gaattaccgg
    10321 taccttgtag gccatcagca ccaccggcag ttcggccggc gctttcaccg tcaggcggcg
    10381 aataccggtt tgctccggct cgtcctgcgg cttgcgcgca ggcagcgcgc gcggcggcag
    10441 cgaaccgtag tacttctcgg ccagtctgaa cacctccatg tgatcaacat cgccgacaat
    10501 cacgacaatg gcgttgttcg gcacgtacca gcgctcgtac cagtcgcggg catcgccgac
    10561 agtcatgttt tccaggtcgt tcatccagcc gatgaccggc acgcggtaag ggtgcgcctg
    10621 cagcgcaact gcattcatct gctcaaagag cagcgactgt ggttggtcgt cggtacgcag
    10681 ccgacgctcc tccataacga ccctgctctc ctgtgcgaac tccttggggt caaggttcag
    10741 gtggcgcatg cgatcggcct cgagctgcat catctgggct agcttctgct tcggcacctg
    10801 ctggaagtag gcagtgtaat ccttgctggt aaaggcgtta tcacgaccgc cctccgccgc
    10861 caccagacgg ctgaattcgc ccgctccggt ggtcggcgtg cccttgaaca tcatgtgttc
    10921 gagcaggtgc gccacaccgg tcgtgccgtt ggtttcgtcc atgctgccgg cgtgatacca
    10981 gaccatgtgc gccgccgttg gtgcacgatg atcctccttg acgataaccc gcaggccgtt
    11041 ggccaactga agctcgaagg ggttggccag ggcatccgcc gggacgacca ggattaatgc
    11101 cagtaacagg ggaatcaatc ttgcgtagcg tctgagcata gatggcttct gataaaattg
    11161 gggaacaggt aacaacaagg caactcgcgg tgcaatctta ccatcgcctc attatccgac
    11221 ccttccagtg acaagaaagc accatgtttg gtttcctcaa gaaatccgct gacccgcaaa
    11281 atgatgctca ggtcagtacc acacctgagc cgacctggcg tgaacgcctc aaaacgggcc
    11341 ttgctcgaac acgcgctcaa tttggcggga agctcaagtc gatcttctct cgcggcaaga
    11401 ttgatgacga gttgcttgag gagctcgaaa ccctgctgct gacctgcgat gtcggcatgg
    11461 aggccacgca gcacttgttg aacgagctca ggatccgggc caaaaagctc aaggtcgaca
    11521 ccccggaggg catccagcag gcgctctcgg ccgccatgca cgacttgctg ctacccctcg
    11581 aaaagccgct tcagatttca tcgcagcgcc ccttcatcat catgatcgcc ggggtcaacg
    11641 gcgcagggaa aaccacctcg atcggaaaac tggcaaagta ttttcacgca cagggcctgt
    11701 cggtcctgct ggcggccggc gacaccttcc gtgcggcagc tcgcgagcag ttgcagacct
    11761 ggggcgagcg caacaatgtg actgtgattt ctcaggagtc cggcgacccg gcggccgtca
    11821 tcttcgatgc catttcggca gccagagcgc gcgggatcga catcgtgctg gccgacaccg
    11881 ccggccgcct gccgacgcaa cttcacctca tggaagaaat cgccaaagtg cgtcgcgtca
    11941 tccagaaagc cgatcccagc ggcccgcatg aaaccctgct cgtcctcgac gccaattttg
    12001 gtcagaacgc cctgcagcag gtcattgctt tcgacaaatc gatcaaggtc accggcctca
    12061 tcgtcaccaa actcgacgga acggccaaag caggcgttgt tgccgccatt gcgcgccagt
    12121 gcccgaagcc gatccgtttc atcggagttg gcgaaggaat cgacgacctg cgccctttcg
    12181 tcgcccgcga cttcgtcgac ggcctgttcg agcaatgagc cttgcctttt cattctcagc
    12241 ccgctcacta cttccctgga catttcgttc cgggctgatc tacggcaatc gttcggattc
    12301 ttgctcatga tcgcctgttc ggccgtaagc aaacgctacc cccaggggct ggaagcactc
    12361 aaggaagtca gcttcgaaat cagtgcgggt gaaatggtgt acttgagtgg ccattccggg
    12421 gcggggaaat cgacgctgtt tcgtctgctg gcggccattg aacgcccgac ttcaggcagc
    12481 attgtggtca acgggcagaa cctcgccgcc ttgcgcagaa gcgcaattcc ttaccttcgc
    12541 cgcaacattg gcctgatctt tcaggatcat aaattgctct tcgaccgcag cgcgctgaac
    12601 aacgtgctgt tgccgctggc cattgtcggt ctgtccgcca aagacgcttc ccgcagggct
    12661 tatgccgcac tcgacaaagt gggacttcat ggccgcgaaa atgcgtaccc gatcgccctt
    12721 tccggcgggg aacagcaacg gctggccatc gcccgggccg
//
LOCUS       DAORPM010000035        12735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1022_length_12735_cov_13.007965, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000035 DAORPM010000000
VERSION     DAORPM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1022_length_12735_cov_13.007965"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLD53_03005"
     rRNA            complement(<1..189)
                     /locus_tag="PLD53_03005"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(512..1702)
                     /gene="tyrS"
                     /locus_tag="PLD53_03010"
     CDS             complement(512..1702)
                     /gene="tyrS"
                     /locus_tag="PLD53_03010"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRC59905.1"
                     /translation="MLSVEDSLALLKRGVDELLIEADLTERLKSGRPLRIKAGFDPTA
                     PDLHLGHTVLINKLKHFQDLGHHVMFLIGDFTGMIGDPTGKNATRPPLSREQILVNAK
                     TYQEQIFKILDPQKTEICFNSTWFEPMGAAGMIKLAALHTVARMLERDDFSKRYRGGQ
                     PIAIHEFLYPLCQGYDSVAMKADVELGGTDQKFNLLVGRELQKHYGQLPQCVLMMPLL
                     EGLDGVNKMSKSLGNYIGIAESPTEIFGKVMSVSDELMWRYFELLSFEPIGKIAAMRL
                     SVEEGANPRDIKFELAKELVNRFHGGSRAGDDAMREFIARFQRSEIPDNMPEVAVAAG
                     SMAAILKQAGLVSSTSEALRMIDGGGVRVDGEKVGDRNARLSVGTCVVLQVGKRKFAR
                     VVVG"
     gene            1791..3119
                     /locus_tag="PLD53_03015"
     CDS             1791..3119
                     /locus_tag="PLD53_03015"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC59906.1"
                     /translation="MNSSKSRILAHSHPAIERLLRHRWLAASLGSAALLAMVTAFALA
                     PSEDANRVQLRTVLEQLSLPATTVVDAENPTFLREEFVQRSDTFYSLIARLGISDQEA
                     LDFIRQNEQAQVIARQLRPGKVVTAKTGEHGKLIALYFPLNSKDAMAVVERRGDGFVA
                     SQESLQLEPRTVIKSGEISSSLFGATDALDIPDAIAIQLAEIFAGDIDFYRDLRKGDK
                     FSLVYETFTHRGQPVRSGRILAAEFINDQKTYNAFWFQAEDGKEAYYTADGKTLRKAF
                     LRSPLEFSRVTSGFTNARFHPVLKTWRAHKGVDYGAPKGTRVRSVADGTVQFAGQQGG
                     YGNLIILKHQGTYSTAYGHLNGFATGVRKGARVHQGDTIGYVGQTGLATGPHLHYEFR
                     VHSNQVNPLAIKLPVAIPLETSQIAHFKATTGPLRAKLELVNQINLALVE"
     gene            complement(3170..3520)
                     /gene="erpA"
                     /locus_tag="PLD53_03020"
     CDS             complement(3170..3520)
                     /gene="erpA"
                     /locus_tag="PLD53_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRC59907.1"
                     /translation="MNTATDMPLPFVFTDSAASKVKALIEEEGNPELKLRVFVTGGGC
                     SGFQYGFTFDELANEDDTSLVKDGVTLLVDPMSYQYLVGAEIDYTEGLEGSQFVIKNP
                     NASSTCGCGSSFTA"
     gene            complement(3566..3967)
                     /locus_tag="PLD53_03025"
     CDS             complement(3566..3967)
                     /locus_tag="PLD53_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRC59908.1"
                     /translation="MFGTKKGSTAQGSIDSLIGVGTRIDGDVVFSGGLRVDGQVHGSI
                     SSENGEQGTVVISEKASVEGAISVGHVVINGTVVGPVFAGESLELLPAARVTGDVEYH
                     QIEMQQGAVIQGRLVHKTSAKPVDLKLATGK"
     gene            complement(3979..4743)
                     /locus_tag="PLD53_03030"
     CDS             complement(3979..4743)
                     /locus_tag="PLD53_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59909.1"
                     /translation="MATSGASIKLKRLRQRFGINAPKLAIRTHVAWYWRALAIIVMLS
                     VSLALAIWIYDAGRRIAGYQSNESEQEIQSLRNRVTELDAELNKLRTLAGSGESSLMI
                     ERATLDQLSRQAKTLELENAALKEDLAFFEGLMPSSVAGEEVGVRIDKLRVEPTGALG
                     EYRYRMLVINNGVRQAKELKGSLQFLVKTQQGGKDVMITLPSGKEPERENYRFELKHF
                     HRLEGVFSVPSGAVVEEVEARLLLDGVVRVRQSVTL"
     gene            complement(4719..5747)
                     /gene="argC"
                     /locus_tag="PLD53_03035"
     CDS             complement(4719..5747)
                     /gene="argC"
                     /locus_tag="PLD53_03035"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRC59910.1"
                     /translation="MVKVGIVGGTGYTGVELLRLLAQHPGVEIVTITSRGDAGTSVCS
                     MFPSLRGRVDLEFEDPLASNLRSCDLVFFATPNGVAMQQAPELLDAGVRVIDLAADFR
                     ITDVAEWEKWYGMLHACPEWVASAVYGLPEVNRVAIREARLVANPGCYPTAVQLGFLP
                     LVEAGLVDLDHLIADAKSGVSGAGRKAEVSSLFSEAADNFKAYGVPGHRHLPEIRQGL
                     SRIAGVGVGLTFVPHLMPVIRGIHATLYSRIKEEADYQSLYELRYANEPFVDVLPAMS
                     HPDTRSVRAANTCRIAIHRPQGGDTMVVLSVIDNLVKGAAGQAVQNMNIMFGLEETDG
                     LWQLPVLP"
     gene            complement(5852..6244)
                     /gene="rpsI"
                     /locus_tag="PLD53_03040"
     CDS             complement(5852..6244)
                     /gene="rpsI"
                     /locus_tag="PLD53_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRC59911.1"
                     /translation="MAENFYYGTGRRKSAVARVFMKRGTGNIVVNGKPVDEFFSRVTG
                     RMIVRQPLVLVEQTAGFDILVNVAGGGESGQAGAVRHGITRALIEYDAELKPVLSKAG
                     FVTRDAREVERKKVGLHKARRRKQFSKR"
     gene            complement(6255..6683)
                     /gene="rplM"
                     /locus_tag="PLD53_03045"
     CDS             complement(6255..6683)
                     /gene="rplM"
                     /locus_tag="PLD53_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRC59912.1"
                     /translation="MKTFSAKAHEVKREWLIVDAADKVLGRLATEIARRLRGKHKPEF
                     TPHVDTGDYIVVTNVEKLRVTGNKAEDKVYYRHTGYPGGIYESSFTQMQKRFPGRVLE
                     KAVKGMLPKGPLGYAMLKKMKCYAGETHPHAAQQPKVLEL"
     gene            7167..7586
                     /locus_tag="PLD53_03050"
     CDS             7167..7586
                     /locus_tag="PLD53_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRC59913.1"
                     /translation="MECDVKWTGNGMSFLAETGSNHIIAMDGAPESGGRNLAPRPMEM
                     LLAGAGGCTAFDVVLILKKGRHAISGCEVKVQAVRADEDPKVFTQIHFHFRVSGTQLK
                     PNAVARAIELSSEKYCSASIMLGKTAAITHDFEIVED"
     gene            complement(7583..8203)
                     /gene="coq7"
                     /locus_tag="PLD53_03055"
     CDS             complement(7583..8203)
                     /gene="coq7"
                     /locus_tag="PLD53_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HRC59914.1"
                     /translation="MLDPVIIGFDRALRTLFATSSTTRRVPGEECPEPALSLNERAHA
                     GALMRINHVGEVCAQALYQGQAITCRDPAIRRALETAAYEETEHLAWTEQRIDELGAR
                     KSLLNPLWYLGALSLGVFAGKLGDAANLGFLAATERQVEAHLNHHLSELPVDDVKSRA
                     IVHQMKLDEISHAETAVRLGARELPPQVVAVMRLAAGLMTRTAYYV"
     gene            complement(8244..8759)
                     /locus_tag="PLD53_03060"
     CDS             complement(8244..8759)
                     /locus_tag="PLD53_03060"
                     /inference="COORDINATES: protein motif:HMM:NF020331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HRC59915.1"
                     /translation="MRKTIWWCLALVLPFSQPSADSFDEDYEVKSWAEIEVQLPAFPE
                     KSNLIPFRVGAITDYQYLIDGKSLSVGSDGVLRFALVVISPSGAQNISFEGLRCATAE
                     RRFYASGRSDKTWSKARSNKWVGIQGGSNNQYVELYKNYFCSVGALEIMNADDARRAL
                     RYGGQPAATRP"
     gene            complement(8813..9358)
                     /locus_tag="PLD53_03065"
     CDS             complement(8813..9358)
                     /locus_tag="PLD53_03065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRC59916.1"
                     /translation="MLDREGYRPNVGIILCNARNEVFWGKRIREHSWQFPQGGIKRGE
                     TPEQAMYRELYEEVGLLPEHVSILGRTKDWLRYDVPIHWVKREWRGSYRGQKQIWFLL
                     RLVGRDSDVSLRACSHPEFDAWRWNHYWVSLDGVIEFKRRVYEQALNELARFIDADHR
                     RPRHEAVRDRHETGGDVGRNE"
     gene            9527..11257
                     /locus_tag="PLD53_03070"
     CDS             9527..11257
                     /locus_tag="PLD53_03070"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRC59917.1"
                     /translation="MRTSKFFISTLREAPSDAEIVSHKLMLRSGLIKRLAGGIYTWMP
                     VGLRVLRKIESIIRQEMDLAGAIELSMPAVQPAELWQESGRWEKYGPELLRLKDRHQR
                     DFVIGPTHEEVITDVVRKEVKSYRQLPLHFYQVQMKFRDEIRPRFGVMRGREFLMKDG
                     YSFHASFEDLQREYRNMFETYTRIFTRLGLKFRAVTADTGSIGGTGSHEFHVLADSGE
                     DALAYCPDSDYAANVELAEAIAPSEPRSSASQTMKKISTPGKTRCEDVAALLSIPVIS
                     MVKAIAIMCQPPDADSAQFALLLLRGDHDLNEVKTQKIVGEFRFARDDEIVEALGCKP
                     GYIGPVGIESIPVFADRSVATMSDFVCGANEEGFHLGGVNFGRDLPEPLAVADLRNVV
                     AGDTSPDGKGKLELCRGIEVGHIFQLRTKYAEALKCVFLDESGQSQAMEMGCYGIGVT
                     RIVGAAIEQNHDDRGIHFPPAIAPFEVCIVPMGYAKSQSVKLAADELYISLKAAGVDV
                     LLDDRNERPGVLFAEMELIGIPHRIVVGDRGLVEGKLEYKGRTDTEPTMVPIAEIGAY
                     LKARLCEESP"
     gene            11239..11937
                     /locus_tag="PLD53_03075"
     CDS             11239..11937
                     /locus_tag="PLD53_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRC59918.1"
                     /translation="MRRVALVLALLLPFTAFAGMQKYEPLSESVRAALSRAISDQAPP
                     RSSFLDSMDAVDWLTEMSRRLEKRIANRESRLEFLRAVHYEATRAGLDPQLVLGLIQV
                     ESGFKKYAVSSAGARGFMQVMPFWIKVIGRSSDNLFHLRTNLRYGCTILRHYLDIEQG
                     DLYRALGRYNGSLGQAEYPNLVRGAWHNQWAYSRDKMKTSAFRATFPSSLASSWPYLR
                     RRTVSSFSAFAQTA"
     gene            complement(11834..>12735)
                     /locus_tag="PLD53_03080"
     CDS             complement(11834..>12735)
                     /locus_tag="PLD53_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRC59919.1"
                     /translation="AQAGVDFFPSSPADKPVEIARNAVDWARRHFHDVLLVDTAGRLA
                     IDELMMKEIAELHAAIEPIETLFVVDAMMGQDAINTAKAFSEALPLTGVILTKLDGDA
                     RGGAALSVRHITGRPIKYAGVGEKLSGLEAFHPERMASRILGMGDVLSLIEDAKKGID
                     EQKAVEFAKKLKSGKSFDLADFKEQIGQMRKMGGLSSLMDKLPSQFAQAASQMPAGGE
                     EKAIGRIEGIIDSMTPAERSKPELLKASRKRRIALGAGVQVQEVNRLLKQFEQTQKMM
                     KQFSGGGMAKMMRGMKGMLPGMR"
BASE COUNT         3010 a         3695 c         3138 g         2892 t
ORIGIN      
        1 tcctgctcac agaatatgcg gtattagcgt aactttcgct acgttatccc ccacttcagg
       61 gtacgttccg atgtattact cacccgttcg ccactcgcca ccaggattgc tcccgtgctg
      121 ccgttcgact tgcatgtgta aggcatgccg ccagcgttca atctgagcca ggatcaaact
      181 cttcagttca attcctgtaa aaaaactctc gcttgacgtc ctgtaatccc ggtttcaccc
      241 agaaccacag tcttttactt catcgtgcga gttacttcat ctcgtcgcgt tatgtaaaaa
      301 cataattaac cagaccaaaa acccacacct atcgattgtc taattgttaa agagcttgca
      361 aatcaaacgg ttagcatgtt tgcagcactg cgtcagcagc gaaggggcgt attgtacagg
      421 ccaaacggtg agcgtcaaca tcttttcaca ataaatttgc aactcttttg caatgcatct
      481 ctaacgtgtt gatctcttgt ctttctttat gtcagccaac aaccacgcgc gcaaattttc
      541 gtttgccgac ctgcaatacg acacacgttc cgacgctcaa gcgggcattc cgatcgccga
      601 ctttttcccc gtcgacccgc acaccgccac catcgatcat gcgtagtgct tcggaagtgc
      661 tcgaaaccag tcccgcctgc ttcaaaatgg ccgccatgga tcctgcagct acagccacct
      721 cgggcatgtt gtcgggaatc tcacttcgtt gaaaccgggc aatgaactca cgcatcgcat
      781 cgtcgcccgc tctggaacca ccatggaaac ggttgacaag ttccttagcc agttcgaatt
      841 tgatatcgcg tgggtttgcg ccttcctcga cgctcaaacg catcgccgca atctttccga
      901 tcggctcgaa ggagagcagc tcaaagtagc gccacatcag ttcatccgag accgacatca
      961 ctttgccaaa tatctctgtc ggcgattcgg caatcccaat gtaattgccc agggacttcg
     1021 acattttgtt cacgccgtcc aatccctcca gcagcggcat catcagcaca cactgcggca
     1081 actgcccgta gtgcttctgc aactcacgcc caaccagcag gttgaatttc tggtccgtcc
     1141 cgccaagttc gacgtcggcc ttcatggcca ctgagtcata gccttggcac agcggataca
     1201 gaaattcatg aatcgcaatg ggctggcctc cacgataacg cttggagaaa tcatccctct
     1261 ccagcatgcg cgcgacagta tgcagtgccg cgagcttgat catgccggca gcacccatcg
     1321 gctcaaacca ggttgaatta aagcaaatct ccgtcttctg cggatcaaga atcttgaata
     1381 tctgctcctg ataagttttg gcattgacaa ggatttgctc gcgcgagagc ggcggacgtg
     1441 ttgcattctt tccggtagga tcgccaatca ttccggtgaa atccccgatc aaaaacatca
     1501 catgatgtcc aagatcctga aaatgcttca gcttgtttat cagtacggta tgccccaagt
     1561 gaagatcggg agcggtcggg tcaaagcccg ccttgattcg aagtggccgg ccgcttttca
     1621 acctttcggt caaatcagct tctatcagta actcatcgac cccacgcttg agcagagcaa
     1681 gactatcttc aacactgagc attcaaagcc tctatcattt actccccaag ggttcatgcg
     1741 gagttatttt tttgttagac tgccgcgaga cttatactcg gtggccgtcc atgaatagtt
     1801 caaagagcag gattctagcg cattcccatc cagcgatcga acgattgctg cgtcaccgct
     1861 ggttggcggc aagccttggt agcgctgcgt tactggcaat ggtgaccgcg tttgcccttg
     1921 ctccctctga agatgcaaac cgcgttcaac ttcggactgt ccttgagcaa ctgtccctac
     1981 ctgcaacgac cgtggttgac gccgaaaacc caacgttcct gcgcgaagaa ttcgttcagc
     2041 gttccgatac tttttatagt ctgatagccc gtcttggtat ctcggatcaa gaagccttgg
     2101 atttcattcg acagaatgag caagctcagg tcattgcccg ccaacttcgt cctggcaaag
     2161 ttgtcacggc aaaaaccggg gaacacggca agctgatagc gctctatttt ccccttaaca
     2221 gcaaagatgc gatggcggtc gtcgaaaggc gcggcgatgg ctttgtggcc agccaagagt
     2281 ctttgcagct tgagccacgc actgtcatca aatccggcga gataagcagc tccctgttcg
     2341 gcgccaccga cgcacttgat attcccgacg cgattgcgat ccagttggct gaaattttcg
     2401 ctggtgatat tgatttctat cgcgacctgc gcaagggtga caagttttcg ctcgtgtacg
     2461 aaacattcac tcaccgcgga caaccggttc gtagcggtcg catcctagcc gccgaattca
     2521 ttaacgatca gaagacgtat aacgccttct ggttccaggc agaggatggc aaagaagctt
     2581 actacactgc ggatggaaag actctgcgca aggcttttct gcgctctccg cttgagtttt
     2641 cccgcgtcac atcagggttt accaacgccc gctttcaccc ggtcctgaaa acctggcgag
     2701 cccacaaagg cgttgattat ggagcaccaa agggtacccg ggtacgttca gttgcggatg
     2761 gaacggttca gttcgcaggt cagcaaggcg gctacggcaa cctgattatc ctaaaacatc
     2821 aaggcactta ttcgacggcc tatggtcatt tgaatgggtt tgccaccgga gttcggaagg
     2881 gtgcccgagt ccaccaaggc gacaccattg gctacgttgg gcaaacaggg ttggctaccg
     2941 ggccgcatct acactatgag ttccgcgtcc actccaacca ggtcaaccca ctggcgataa
     3001 agcttcctgt ggcgattcca cttgaaacgt cgcaaattgc ccacttcaag gccacgaccg
     3061 gaccattgcg ggcgaagctg gagctggtga atcagatcaa tttggcccta gtcgaatgaa
     3121 agaaagcgac tagagtcgct ttctttcatt cccttcttga cttcgggtgt caagcagtga
     3181 aggaggagcc gcacccacag gtcgaggatg cattcgggtt cttgatcacg aactgcgaac
     3241 cttcaagccc ttcggtatag tcaatttcgg caccgacaag atactggtaa ctcattgggt
     3301 caaccagtag ggtcaccccg tccttcacca atgacgtgtc gtcctcgttg gcaagctcat
     3361 caaacgtaaa gccatactgg aatccagagc agccaccacc cgtcacgaac acgcgaagtt
     3421 tcagttccgg gttcccttcc tcctcaatca gagccttgac cttgctggcc gcgctgtccg
     3481 taaaaacaaa cggcaacggc atatccgtag ctgtattcat tgattatctc tccaaactct
     3541 cgcaaaaaaa actgcaggaa gcacctcatt taccggtagc cagcttcaaa tcgacaggct
     3601 tggcactcgt cttatgtaca agacgcccct ggatcacagc accttgctgc atttcgatct
     3661 ggtgatactc tacatcccct gtgacacgcg cagcaggcaa gagttccagg gattcaccag
     3721 caaatacggg acccaccacg gtaccgttga tcaccacatg accaactgat atcgcgcctt
     3781 ccacactcgc cttttcgctg atgaccactg ttccttgctc accgttttca ctactgatgc
     3841 tcccgtgaac ctgaccatcg acacgcaacc ctcccgaaaa cactacgtcc ccgtcgatgc
     3901 gtgtaccaac gccgatcagg ctgtcaatgc tgccctgcgc tgtgctaccc ttttttgtgc
     3961 cgaacatgaa ttctccgtct aaagagtcac agactgccgt acgcgcacga cgccatccaa
     4021 tagcaagcgc gcctcgacct cttctacaac agcaccagag ggtaccgaga aaacaccttc
     4081 gagccgatgg aagtgtttta gttcgaaacg ataattctcg cgctctggct ccttcccgga
     4141 aggcaaggtg atcataacat ctttgccccc ttgttgcgtt ttgaccagaa actgtagcga
     4201 gcccttgagt tctttggctt gacgaacgcc gttattaatc accagcatac gatagcggta
     4261 ctcgcccaag gctccagttg gctcgacccg cagtttgtca atccggactc ccacttcctc
     4321 gccagccacg gaagatggca tcaggccctc gaagaacgca aggtcctctt tcagtgctgc
     4381 gttctccagt tcgagcgtct ttgcctggcg tgacaattgg tccagtgtgg cacgctcaat
     4441 catcagactg ctttcacccg aaccagcaag tgtgcgcaac ttgttcaact cggcatcgag
     4501 ctccgtaaca cgattcctga gcgattgaat ttcctgttcc gactcattgc tttgatatcc
     4561 ggcgatgcgc ctacctgcat cgtatatcca aatcgcaagt gccaatgaaa ctgacagcat
     4621 cacaattatt gccaatgcgc gccaatacca ggctacatgc gttctgatcg ccagcttcgg
     4681 agcattaatc ccgaatcgtt ggcgcaatcg tttgagtttt atggaagcac cggaagttgc
     4741 catagtccgt cagtttcctc caagccaaac atgatattca tgttctgtac ggcctgacca
     4801 gccgctccct tgacgagatt atcgataacc gaaagcacga ccatcgtatc cccaccctgc
     4861 ggcctgtgaa tggcgatacg gcaagtattc gctgcgcgaa cggagcgtgt atccgggtga
     4921 gacatcgccg gcaagacatc aacgaacggc tcgttcgcat aacgcaattc gtaaagtgac
     4981 tggtaatcag cttcttcctt gatccgagaa tacagcgtcg catgaatacc tcgtatcacc
     5041 ggcatcaaat gcggcacgaa ggtcaaaccc acaccaacac cggcaatccg tgacaaaccc
     5101 tgccgtatct caggcaagtg gcgatgccct ggaacgccgt acgccttgaa attgtcagct
     5161 gcttctgaaa acagcgatga aacctcagct tttcgtccag cccccgacac gccagattta
     5221 gcatcggcaa tcaaatgatc cagatcaact aggccggcct caactagcgg caggaaaccc
     5281 aactgaaccg cggttgggta acaccctgga ttcgcaacca gacgcgcttc acgaattgcg
     5341 acgcggttaa cttccggcaa accgtagaca gcacttgcga cccactccgg gcaggcgtgc
     5401 aacatgccgt accacttttc ccattcagcg acatcggtaa tgcggaaatc cgccgcaagg
     5461 tcaatgacac gaactcccgc atcgagcaac tcgggagcct gctgcatggc tacgccattg
     5521 ggggtagcaa agaagacgag atcacaactc cgcagattgg aagctagcgg atcttcgaac
     5581 tccaaatcaa cccgaccgcg taagcttggg aacatgctgc aaacggacgt ccccgcatcg
     5641 ccacgcgaag taattgtgac tatttccacc ccaggatgct gcgcaagcaa gcgcagcagt
     5701 tcgacgcccg tatacccggt gccaccaaca attccaacct tgaccatcat ttcctcctca
     5761 acaggggtgc aatgatagtc tgcgtgcccc aaaatgacaa aaccgcccga gggcggcatt
     5821 gtgttttgaa ctcgggagaa tccagcgcaa cttaacgctt ggaaaactgc ttgcggcgac
     5881 gtgctttgtg cagaccgacc ttcttgcgct caacctcacg ggcatcgcgg gtaacaaaac
     5941 ctgcctttga caacacgggc ttgagctccg cgtcatattc aatcagagca cgggtaattc
     6001 cgtgacgtac agcgcccgcc tggccagatt cacccccccc ggcaacattc acaagaatgt
     6061 caaaaccagc ggtttgttca accaaaacga gcggctgacg aacgatcatg cggcccgtaa
     6121 cacgagaaaa gaactcgtct accggcttgc cattgacgac gatgttgcct gtgccacgct
     6181 tcatgaaaac gcgcgccacg gcactcttgc gacggccagt gccgtagtag aaattttcag
     6241 ccattttcga tcccttagag ttccagaact ttcggctgct gagcagcgtg agggtgcgtc
     6301 tcccccgcgt agcacttcat ctttttcaac atggcgtaac caagaggccc tttaggaagc
     6361 atacccttga cggccttttc gaggacacgc cctgggaaac gcttctgcat ctgcgtaaag
     6421 ctcgattcgt agataccgcc ggggtaacct gtatggcggt agtacacctt gtcttcggcc
     6481 ttgttgccag tgacacgcaa tttctccaca ttggtcacca cgatgtaatc accggtatca
     6541 acgtgagggg taaattctgg tttgtgctta ccacgcaggc gccgcgcgat ttcagtcgcg
     6601 aggcggccaa gcaccttgtc agcggcatca actatgagcc actcgcgctt cacttcatgc
     6661 gctttggcgg agaaagtctt cattaaaacg ccgtccttat atgccagata acggaaagcc
     6721 gggcattcta ttttagattc tcgaatcttg tcaatcgctg attctattgc cggcaaaaaa
     6781 aacgcgactc caaagagtcg cgcaaaatcc actcctaagg aggagggtgg aggaggagac
     6841 aaatctgaaa catgctgaat tgcccacata cactgcaata gagcaattgg aagcttaccc
     6901 tacccttacg caaagatcaa gcaatattgt gcattgcgtc acaagaactg attaggcacc
     6961 gttcacgcac aacaacacaa caacttttca ttttttatca tgtcgttatg gcttgtatag
     7021 ataacacgtt acgcaataag caatttatgg ggcgtaataa ttgtacaagc tttgcactgt
     7081 tgttgttgca acgcagcatt gcgcggacgc ggcgcagaag cacacttcca gctagaatga
     7141 gcgctttcaa gagaggcgag gattgtatgg agtgcgatgt gaaatggacc ggaaacggca
     7201 tgtcattttt ggccgaaaca ggaagcaatc acatcatcgc aatggatgga gcccctgaat
     7261 ccggtggtag aaacctggct ccacgaccga tggaaatgct gcttgccggc gcaggaggct
     7321 gcacggcatt tgacgtcgta ttgattctaa agaaagggcg tcacgcaatc tcgggatgtg
     7381 aagtcaaggt gcaggcagta cgtgctgacg aagaccctaa ggtattcaca caaattcatt
     7441 ttcacttccg cgtcagtgga acacagctga aaccaaatgc ggttgcacgc gcgatcgaat
     7501 tatcgagcga gaaatactgc tctgcctcaa tcatgcttgg taaaactgcc gcaattacac
     7561 acgatttcga gatcgtcgaa gactagacgt agtaggcggt gcgtgtcatt agacctgccg
     7621 ccagtcgcat gaccgcaacc acttgtggag gcagttcgcg cgcgccgagg cggacggccg
     7681 tttcggcgtg ggatatctcg tctagtttca tttgatggac tatcgctctg gacttgacgt
     7741 catccactgg caattcggac aaatggtgat tgaggtgggc ttcaacctgt cgttcggtcg
     7801 ctgccagaaa gccaagattt gccgcgtcac cgagcttgcc agcgaacacc cccaaggaca
     7861 atgcccccag ataccacagg gggttgagca gactcttgcg tgcccccaat tcatcgatac
     7921 gctgttcggt ccaggccaga tgttcggttt cctcataagc agctgtttcg agggcccgtc
     7981 ggattgcggg gtcgcggcag gtgatggcct gaccctgata aagagcctgc gcgcaaactt
     8041 ccccgacatg attgatgcgc atcagtgcac ccgcatgagc cctttcattc agagacaagg
     8101 ctggctccgg gcattcctcc ccgggcactc ttcgggtagt cgacgaagtc gcgaaaaggg
     8161 tgcgcaaagc cctgtcaaaa ccaatgatca cgggatcaag catattggaa gccaatctgt
     8221 gggtagcacg tcaaaccaaa aacctaaggg cgcgtcgccg ctggctgtcc accgtagcgc
     8281 agggcccggc gagcatcgtc ggcattcatg atctcgagag caccgactga acaaaaatag
     8341 ttcttgtaaa gctcgacata ctgattgttg ctaccgccct gaatcccgac ccacttattg
     8401 ctccgcgcct tcgaccaggt cttgtctgat cggccagacg cataaaacct gcgctcagca
     8461 gtagcgcaac gcaaaccttc aaaactgatg ttctgggcgc ctgaagggct gatcaccacg
     8521 agagcaaaac gcaacacacc gtcagagcct acggatagcg atttgccgtc gatcaggtat
     8581 tggtaatcgg taatggcgcc aacccgaaac ggaataaggt tgctcttttc cgggaaggct
     8641 ggcagctgca cttcgatttc cgcccaggat ttaacttcgt agtcctcgtc gaagctatcc
     8701 gccgatggct gcgaaaatgg caacaccaaa gccagacacc accaaatagt tttccgcaac
     8761 cgcatcaacg cccgctccag ctagatcaag taaacgcgtg tgagtaagaa aatcattcgt
     8821 ttcgccccac atcgccacca gtctcatgac gatcacgaac tgcctcatgt cgcggccgac
     8881 ggtgatccgc gtcgataaag cgcgcgagct cgtttaacgc ttgttcatac actcggcgtt
     8941 tgaattcgat gacgccatca agcgaaaccc agtagtgatt ccaccgccag gcgtcaaact
     9001 cgggatgact acaagcacgc aaagacacat cgctatcacg ccctaccaga cgtagcaaaa
     9061 accatatctg tttttgccct ctgtaactac cgcgccattc acgttttacc cagtgtatcg
     9121 gcacatcgta acgcaaccaa tccttggttc tgccaagaat gctgacatgc tcggggagca
     9181 aacccacctc ttcataaagt tcccggtaca tcgcctgctc aggagtttca ccccgtttga
     9241 tgccaccctg tggaaactgc catgaatgtt cgcgtatacg tttaccccaa aagacttcgt
     9301 tccttgcgtt acataggatg atgccgacgt tagggcggta accttcacgg tcgagcattg
     9361 tgtttacctg caaaatttat cggtcgtgcc cattcttcca catttaatag ggcttggaaa
     9421 gcgtgccaaa gcccgacacg cccaaaccgc cgacgaacgc gattagcatc gcgctctact
     9481 gatctgtctt gtaaaattcc actttatttc cagatgattg attcccatgc gcacgtcgaa
     9541 gttctttatt tcaactctcc gggaggcgcc ttccgatgca gagatcgtca gccacaaatt
     9601 gatgttgcgc tcaggtctga tcaaacgctt ggctggcggc atctacacct ggatgccggt
     9661 tggccttcgc gtgctacgca agatagagag catcatccgc caggaaatgg atcttgcagg
     9721 cgccatcgaa ctttcgatgc cggccgtaca accggccgag ttgtggcagg aatcggggcg
     9781 ctgggaaaag tacggccccg agctgcttcg tctgaaagac cgccatcagc gtgatttcgt
     9841 catcgggccg acgcacgaag aggtcattac cgatgtcgtg cgcaaggaag taaagagcta
     9901 tcgccagcta ccgctacatt tctaccaggt gcaaatgaag ttccgtgacg aaatccgccc
     9961 gcgctttggc gttatgcgcg gtcgcgagtt cctgatgaag gacggctatt cattccatgc
    10021 cagtttcgag gacttgcaac gcgagtaccg caacatgttc gaaacctaca cgcgtatctt
    10081 cactcgtcta ggattgaaat tccgcgccgt aactgcggac actggatcga taggcggaac
    10141 gggttcgcat gaattccatg tgctggccga ttccggcgaa gacgcactgg cttactgccc
    10201 tgactccgac tacgctgcca acgtcgaact ggctgaagct atcgcaccga gtgaaccacg
    10261 tagttcagcg tcccaaacca tgaagaagat ttctacaccg ggcaagacaa gatgtgagga
    10321 tgttgctgcc ttactcagca tccctgtgat atccatggtc aaagccatcg ccatcatgtg
    10381 ccaaccgccg gatgctgact ccgcccagtt cgcgcttctc ctgcttcgcg gcgaccacga
    10441 cttgaacgaa gtcaaaacgc agaagatcgt tggcgagttc cgttttgcgc gcgacgatga
    10501 aattgtcgag gctctgggct gcaagccagg atatattggc ccggtcggca ttgaatccat
    10561 tcccgtcttc gccgatcgaa gcgtagcgac gatgagcgac ttcgtttgcg gtgccaacga
    10621 agaagggttc catcttggcg gcgttaattt tggtcgtgac ctgccggagc cattagctgt
    10681 cgccgacctg cgaaacgttg tcgcaggcga cacttcaccc gacggcaaag ggaagctcga
    10741 actgtgtcgc ggcattgaag ttggtcacat ctttcagtta agaacaaaat atgccgaggc
    10801 cctaaaatgc gtctttctcg acgaaagcgg gcagagtcag gccatggaga tgggttgtta
    10861 tggcatcggt gttacgcgaa ttgttggcgc ggccattgaa cagaaccacg atgatcgcgg
    10921 catccatttt cctccagcga tcgcaccctt cgaggtgtgc atcgttccca tgggatacgc
    10981 caagagtcag tccgtaaagc tggctgcgga cgaactctac atcagcctta aggcagcagg
    11041 cgtcgatgtt ctgctcgatg accggaacga gcgtcccggt gttttgttcg cggagatgga
    11101 gttgattggc atcccacacc gtatcgtcgt cggcgatcgc ggcttggtgg aaggcaaact
    11161 cgaatacaaa ggccgcaccg atacggagcc gacaatggta cctatcgctg aaatcggtgc
    11221 ttacctgaag gccaggttat gcgaagagtc gccctagtcc tggccctttt gcttcccttc
    11281 accgcgtttg cgggcatgca aaaatacgag cccctgtcgg aaagtgtacg agcggcgcta
    11341 tcgcgcgcca tttctgacca agccccgcca agaagctcat ttcttgattc catggacgcc
    11401 gtcgactggc tgaccgagat gtctcgtcgc cttgaaaaac gcattgccaa tcgtgaaagc
    11461 cgactggagt ttcttcgtgc cgttcactac gaagcgacgc gcgccgggct cgacccgcaa
    11521 ctcgttctgg gcttgatcca ggtcgagagt ggattcaaga aatacgctgt ctcgtccgct
    11581 ggcgctcggg gcttcatgca ggtcatgccc ttctggatca aggtcatcgg ccgcagcagc
    11641 gacaacttgt tccacttacg aaccaatctg cgctacggct gcacaatcct ccgccactac
    11701 ctggacatcg aacaaggcga tctctaccgg gcacttggtc gttacaacgg cagtctgggg
    11761 caggccgaat acccgaatct cgttcgtggc gcttggcaca atcagtgggc ctattccaga
    11821 gacaagatga aaactagcgc attccgggca acattccctt catccctcgc atcatcttgg
    11881 ccatacctcc gccggagaac tgtttcatca ttttctgcgt ttgctcaaac tgcttgagca
    11941 aacgattgac ttcctgcacc tgaacgccgg ctcccaaagc gattcggcgt ttacgcgagg
    12001 cctttaacaa ctcgggcttg ctacgctcgg caggtgtcat cgaatcgata atgccttcga
    12061 ttcggccaat cgctttttct tcgccgccag ccggcatctg actagcggcc tgtgcgaact
    12121 gtgacggcaa tttgtccatc agcgacgaaa gcccgcccat tttgcgcatc tggccgatct
    12181 gttccttgaa gtcggcaaga tcaaagctct tgcccgattt gagcttcttg gcaaactcga
    12241 ccgctttttg ctcgtcaatc cctttcttcg catcctcgat caacgacagc acgtcgccca
    12301 tgccaaggat acgggaagcc atacgctcgg ggtgaaaggc ttcaagcccc gacagtttct
    12361 caccgacccc ggcgtacttg atcggccgcc cggtgatatg tcggaccgag agcgctgcgc
    12421 cgccacgcgc gtccccatcc agtttggtga ggatgacacc ggtcagcggc aaggcctcgc
    12481 taaacgcctt ggcggtattg atagcatcct ggcccatcat ggcgtcaaca acgaacagcg
    12541 tttctatcgg ttcgattgcg gcgtgcagct cggcgatttc cttcatcatc aactcgtcga
    12601 tcgccagacg accggcggta tcgacaagca gcacgtcgtg gaagtgccgt cgtgcccaat
    12661 cgactgcatt gcgcgcgatt tcgacaggct tatcggctgg cgatgaaggg aaaaagtcta
    12721 ccccggcctg tgccg
//
LOCUS       DAORPM010000036        12600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1046_length_12600_cov_11.389558, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000036 DAORPM010000000
VERSION     DAORPM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1046_length_12600_cov_11.389558"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..2635)
                     /locus_tag="PLD53_03085"
     CDS             complement(167..2635)
                     /locus_tag="PLD53_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC59920.1"
                     /translation="MRESAYYDALGCLVMRLDRKTAIRFMNRFGLKLLGYERLDQLFG
                     RPMSVVLPDNDAQSATLAALIGNSERVFPDPFEAELLHCDGSLIPLSWKRSEQIGAAG
                     QAAATILVGFDARSMRESQATAAMFQTVTDNYSGSILIANEDNSILYANPALLHMTGY
                     SFEELIGKTPAIFKSGQTPDEVYRNLWETIDSGGIWNGEVINRRKDGSHYLECKTIAA
                     IRDSQGHVQNYFAIGEDGSQRHQLQQHIDKLLAIDQLTGLPNRTAFMNLLVAALNSAY
                     LDESEVTVLHIDIDDFFIVNDAIGTDEADLVIVEIALSIKGALRQADILARLGNDKFA
                     ILLGPHESGIDNVIHDVTGRVLAAIRQPILLTDRPIYVTASIGIAGYPIDGGSASELL
                     SHAMNATERAKAGGGNHCCRFDATTASSASGQRELISDLRLAIERNEMFLHFQPQVSL
                     FSGSIIGLEALIRWRHPARGMISPGEFIPLAEQSNLVVDIGEWVLGETLRQMRAWSDA
                     GLPSIKVAINLAARHLLAPDLHTTIGNALALQRIDPHHLEIEITEGAMMQDIAGAIRS
                     TAQLKKVGVRISLDDFGTGYSSLAYLSRFPIDVVKIDQSFVSDITTNPVNAAIAQATI
                     AMSHKLGKVVLAEGVETEEQMQYLRRNECDEMQGYFFSRPLPAEDIGRLLQEGSTMNL
                     YAQRGSVKRNTVLFVDDEENILASLKRTLRREGYEILTAGSAAEGLSLLATNDVQLIV
                     SDQRMPEMNGTEFLSRVKSLYPQTVRMVLSGYSEISAVTDAINKGAVYRFLLKPWDTE
                     MLKEEIAGALRHWRELYASRRKDD"
     gene            complement(2730..3320)
                     /locus_tag="PLD53_03090"
     CDS             complement(2730..3320)
                     /locus_tag="PLD53_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC59921.1"
                     /translation="MSRILIVDDEESILKSLRRLLSLTPCTAGEVTYKLTVDICSSPK
                     EALEKARHTAYDLVLSDFRMPVMNGVQFLKALREIQPDAARLILSGYADLNGLIDAIN
                     EAGIIRFISKPWNDYELVSTLGQVLALRDLSLENQRLADQARLTMGSISEEEIERKRL
                     EASEPGITQVNWGPDGSVLLDESLLEPESPGDKWLN"
     gene            complement(3333..4640)
                     /locus_tag="PLD53_03095"
     CDS             complement(3333..4640)
                     /locus_tag="PLD53_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC59922.1"
                     /translation="MNDTLDTATTVLLVDDEPGILSSLRRLLRPAGYRIFTAESGKAG
                     LEVLEHEPVDLVISDMRMPEMDGAQFLEQVRNRWPKTTRILLTGYADVASTIAAINRG
                     EIYRYVSKPWDDDDLKLVVRDALEGARLRNENIRLLALTQAQNEELKDLNLNLEDKVR
                     QRTSEIEQVNSFLNLANDKLKQNFLVSIKIFSSLMELRGGAMAGHSRRVADLARKLAI
                     RLGVESKELQDIFFAALLHDVGKIGFPDSLLIRAVSKMGSEELGQYRKHVVAGESALM
                     PLEELKDVSRIVRAHHEQFDGKGFPDGLEGADIVRGARILSVVNDYDGLQIGTLSDKR
                     MSPDEAKALIIQSSGKRYDPQIVDAFVEMLGGLALESDRAKTVSHADLKVGMVLARDL
                     LSKEGIMLLAADFVLDVAVIKKIQEYARRENHAITIYIRTDKH"
     gene            4870..5541
                     /locus_tag="PLD53_03100"
     CDS             4870..5541
                     /locus_tag="PLD53_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC59923.1"
                     /translation="MNTLATHSVPLANAETRVPRRRRKDARPSELTAAALELFVERGF
                     ATTRLEDVAARAGVSKGTLYLYFDSKEALFKAVIEEGIVPTLVAAEQQLAEHSGSAAE
                     LLRKLLFGWWQQIGGTRLAGVPKLIISESRNFPEVAQYYHDKVILRGRALLRTVLQRG
                     IADGEFRALDVETAIDVIIAPLLMLVIWRFSLCFCGQETDPESYLETHFDLLVHGLRR
                     SQDGQ"
     gene            5695..6360
                     /locus_tag="PLD53_03105"
     CDS             5695..6360
                     /locus_tag="PLD53_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRC59924.1"
                     /translation="MNIEKARFNMIEQQIRPWNVLDPSVLSLLSVVKREDFVPAAHKD
                     LAFADIEIPIGAKGNASQTMLAPKIEARMLQELAIRNTDKVLEIGTGTGYMAALLAAK
                     AEYVYSVEIDPGLAEIARNNLQQAGVANVSVEIGDGVQGRSSDAPYDVIVLSGSLPVL
                     PERLLNQLKIGGRLAAVVGEAPVMQLQVITRTSEDAFSTNPIFETVVAPLTNAAQNNR
                     FVF"
     gene            6382..7722
                     /locus_tag="PLD53_03110"
     CDS             6382..7722
                     /locus_tag="PLD53_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRC59925.1"
                     /translation="MRLKLSPSRSSLLLAALFSAAPAFSTDLMQVYRDALTNDQQYMA
                     ARATADALREKEPQGLSGLLPTISGTANTTWNENKYSASNQPQKINRDFNTNAYNVNL
                     TQPLFRWQNIVQYSQGKLQVVQAEANFAQASQDLILRVAQAYFDVLYAEENLKAVRAN
                     KQSIAQQLEQAKKNFEVGTATITDSQEAQSRFDLASAQEIAAENDLEVKRFALSVIIG
                     KHPGELNRLKPQAELKPPQPASMDPWVDAAEKDSFVVQAQQAAAEVAAKEVERNRAGH
                     YPTLDVVANYGKNNSALSFLGNDSLETTARNIGLQLNIPLFQGGYVNSKTREAAANRS
                     AAQATLENTKRTAALNARQSYLGVVNGLAQVRALNAALISSTSALESNKLGYEVGVRI
                     NIDVLNAENQVYITKRDLARARFDTLLSQLRLKAAVGSLGEADLEQINSLFEAP"
     gene            complement(7697..8974)
                     /gene="waaA"
                     /locus_tag="PLD53_03115"
     CDS             complement(7697..8974)
                     /gene="waaA"
                     /locus_tag="PLD53_03115"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HRC59926.1"
                     /translation="MMERQVYSLLFYLIQPLIWLRLLWRARKQPEYLQNLGERYGHYV
                     QPAPARLLWLHAVSVGETRAAEPLIQALLDEYTDHSLLLTHMTPTGRATGAELIAKYG
                     SRLIQAYLPYDLPGASGRFLDHFKPCLGLLMETELWPNLIAGAKARRMPLLLVNARLS
                     ARSQRGYRRLLNLFRPAFDSLRAVAAQTPADAERLMSIGAQRVSVVGNLKFDVIPSPA
                     KLQLGSQWRQKLGARPVWLAASTREGEESLIVDAFVRLNRPDLLLLLVPRHPQRFREV
                     RTLIEERGLTLCRRSENSLPVSETRVWLGDSMGEMPAYFTVADVALIGGTLLPFGGQN
                     LIEAAACDCPALVGPHSFNFSQATDDAIACGAAMRIADADDAANKVLRLLNDRAALQA
                     MRDAAAGFGQAHRGATSRTVALIREIKAPRTAN"
     gene            complement(8971..9921)
                     /gene="waaC"
                     /locus_tag="PLD53_03120"
     CDS             complement(8971..9921)
                     /gene="waaC"
                     /locus_tag="PLD53_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HRC59927.1"
                     /translation="MVKTSSLGDVIHNLPVVSDLRRCFPNAQIDWCVEEAFSDIPRLH
                     PEVSEIVPVALRRWRKTLTQWATWREIGKFRKRLGQTYYDVILDTQGLIKSALIARQA
                     KGRHCGYAAEVARESLAALFYDETFVIPPNAHAVERNRWLAAAAFEIPVDLPLNYGIS
                     TPAVNLSWFVGSRYAVLLTATSRDDKLWDETNWKTIAQNLFKRGLTPVFPSGNAIERQ
                     RVERIVSNVPGAIVAPPLKLNELACLIGGSALAIGVDTGLVHLATALRVPTIAVFVAS
                     DPALTGVYGSGFIRNLGAEGQAPTASEVLTVAEQGARWIR"
     gene            10033..11100
                     /gene="rfbB"
                     /locus_tag="PLD53_03125"
     CDS             10033..11100
                     /gene="rfbB"
                     /locus_tag="PLD53_03125"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRC59928.1"
                     /translation="MILVTGGAGFIGANFVLDWLRESDELVLNLDKLTYAGNLENFGE
                     LVGDPRYAFVHGDIGDQKLVEGLLLKHRPRAVINFAAESHVDRSIHGPGEFIETNIVG
                     TFKLLESVRAHFSAMNDSERVAFRFLHVSTDEVYGSLSAADPAFTESNRYQPNSPYSA
                     SKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIVNALAGQPLPVYGDG
                     QQIRDWLYVTDHCDAIRHVLASGRIGEIYNIGGWNEKPNLAIVHAICDLLDELRPRAD
                     GGRYSEQITYVADRPGHDRRYAIDAGKIGQEIGWRPRETFATGIRKTVQWYLDHQGWV
                     SNVQSGAYREWVEKNYGGRVE"
     gene            11097..12002
                     /gene="rfbD"
                     /locus_tag="PLD53_03130"
     CDS             11097..12002
                     /gene="rfbD"
                     /locus_tag="PLD53_03130"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRC59929.1"
                     /translation="MKILLFGKRGQVGWELQRSLAPLGQVIAIDLDHVGADGLCGDFT
                     NRPGLAGTVRRVKPDVIVNAAAYTAVDKAESDHDLAHRINATAPGVLADEAQEIGAWL
                     VHYSTDYVFDGRGTTPWLETDATRPLSVYGQTKLEGETAVARCDKHLVFRTSWVYAAR
                     GQNFARTMLRLAQERDQLKVIDDQIGAPTGAELLADVTAHALRTVENRTELAGIYHLA
                     AGGETSWHGYAKFVIEQARHAGQQVRVAPEAIEAILTSDYPTPAERPLNSRLDTRKLQ
                     ATFGLILPEWKTGVARMLTEVIGQA"
     gene            complement(12040..>12600)
                     /locus_tag="PLD53_03135"
     CDS             complement(12040..>12600)
                     /locus_tag="PLD53_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRC59930.1"
                     /translation="PLIIGQPARREAVIRDIKQKSGRSGSLAFVSVVCSYYQNDVLCL
                     EEEQDIVYREPGAAVANPQVIAWSPAAENAWTKIVTPDPRLLFRFSALTFNAHRIHYD
                     RPYATSEEGYPGLVVHGPLTAVLLMELVRHHAARPVSKFSFRGLAPLFDLAPFRLVGI
                     PDDDRVSLEAHGPDGSVAMSASAEFA"
BASE COUNT         2698 a         3554 c         3675 g         2673 t
ORIGIN      
        1 acactggagt tgccccccga attggctgca ctgcagtgga ccaatgtgct gacgggcgag
       61 agccatgcac ccgccggaca actggaggtg gggcggcttc tggacttctt cccggtggct
      121 ctgctgacgg cggaggcttc aacttgacgg gcgggttacg cccggatcag tcgtcctttc
      181 tccgcgatgc gtagagttca cgccaatggc gcagtgcgcc agcgatttcc tctttcagca
      241 tttcggtatc ccagggcttc agcaggaagc gataaactgc gcccttgttg atcgcatcgg
      301 tgacggcaga aatttccgaa taaccggaca agaccatacg caccgtttgc ggatagaggc
      361 tctttacccg tgaaagaaac tcggtgccgt tcatttccgg catgcgctgg tcggagacga
      421 tcaactgaac gtcgttggtg gcgagtagcg agagtccttc ggcggcgctt ccggcggtca
      481 ggatttcgta gccttcgcgg cgcagagtgc gcttgagcga tgccaatatg ttctcttcgt
      541 cgtcaacgaa cagcacggtg ttgcgtttga ccgaacctcg ctgggcgtaa aggttcatcg
      601 tcgaaccttc ctgcagcaag cggccaatat cctccgccgg aagcggcctg gaaaagaaat
      661 aaccctgcat ctcgtcacat tcattgcgcc gcagatactg catctgctct tcggtttcaa
      721 caccctcagc cagcaccacc ttgcccagtt tgtgcgacat ggcgatcgtt gcctgcgcaa
      781 tagccgcatt gaccgggttg gtggtgatgt cgcttacaaa ggactggtcg atcttgacca
      841 cgtcgatcgg aaagcgtgag agataggcga gcgaagaata gccggtgcca aagtcatcga
      901 gcgagatacg cactccgact ttcttcaatt gcgccgtgct gcggattgcc ccggcgatat
      961 cctgcatcat ggcaccttcg gtaatctcga tttcgaggtg gtgcgggtcg atgcgctgca
     1021 aggccagcgc gttgccaatc gtggtatgca gatcgggcgc aagaaggtgg cgggcagcca
     1081 ggttgatggc cacctttatg gatggcaggc cggcgtccga ccaggcacgc atctgccgaa
     1141 gggtttcgcc cagcacccac tcgccaatgt cgacgacgag attgctttgt tccgccaggg
     1201 gaatgaactc gccgggcgat atcatgccgc gcgcaggatg ccgccagcgg atcaatgctt
     1261 ccaggccgat aatggagccc gagaacaggc tgacctgcgg ttggaagtga agaaacatct
     1321 cgttgcgctc gatggccaga cgcagatcgc tgatcagttc gcgttgtccg cttgcgctac
     1381 tggccgttgt ggcatcaaaa cggcagcagt ggttgcctcc gcccgctttg gcccgctctg
     1441 tggcgttcat ggcatggctg agcagttcgc tggcgctacc accgtcgatc gggtagccag
     1501 cgatacctat cgaagccgta acgtagatcg gtctgtctgt cagcaagata ggttggcgaa
     1561 tggctgccag cacacgcccg gtcacatcat ggataacatt gtcaattccc gattcgtgcg
     1621 gcccaagcag aatggcaaac ttgtcgttac ccaggcgagc cagaatatcg gcctgtcgca
     1681 aggcgccttt gatactcaag gctatctcga cgataaccag atccgcttca tcggtgccga
     1741 tggcatcatt gacaatgaaa aaatcatcga tatcgatgtg cagaacggtc acctcgctct
     1801 cgtccagata ggcgctgttc aatgcagcga ccagaagatt catgaaggcg gtgcgattgg
     1861 gcaagccggt tagctgatcg attgccagaa gcttgtcgat gtgctgctga agttgatggc
     1921 gctgcgaccc gtcttcaccg atggcgaagt agttttgcac atggccctgc gaatcacgaa
     1981 tcgccgcaat ggtcttgcat tccagatagt ggctaccgtc tttccgtcgg ttgatcactt
     2041 caccgttcca gatcccgcct gaatcgatgg tttcccagag gtttcgatag acctcgtcgg
     2101 gcgtctgccc ggacttgaaa atggcaggcg ttttaccgat cagctcttcg aaggaatagc
     2161 cggtcatgtg caagagggcg gggttggcgt agaggatgga gttgtcctca ttcgcaatga
     2221 ggatgctgcc ggagtagttg tcggtcaccg tctgaaacat ggccgctgtg gcctgactct
     2281 cgcgcatgga gcgcgcgtcg aagccaacca gaattgttgc cgcagcttgc cccgccgcgc
     2341 cgatttgctc gctgcgcttc caggagagcg gaataaggga gccatcgcag tgcagcagct
     2401 cggcctcaaa tggatcggga aacacccgct cagaattgcc gatcaacgca gccagagttg
     2461 cgctttgcgc atcgttgtcc ggcaacacta cggacatcgg cctgccgaac agctgatcca
     2521 gccgttcgta ccccagaagt ttcagcccga agcgattcat gaaccggatt gccgttttcc
     2581 ggtcaagccg catcaccagg cagccgagcg catcgtagta ggccgattcc ctcatggcgt
     2641 gagtcatgcc tcaatagcca ttgttatagc gtctttccgg gcagatcgct cccggagagt
     2701 gaatgcccgc tttagggcgc ctgcatatgt cagttgagcc acttgtcgcc aggactttcg
     2761 ggttcgagca acgattcatc gagtagcacc gagccgtccg gcccccagtt aacctgcgtg
     2821 atacccggtt cggacgcttc gagacgcttg cgctcaattt cctcttccga aatgctaccc
     2881 atcgtcagcc gtgcctgatc tgccagccgc tggttttcaa gcgatagatc gcgcagagcc
     2941 agaacctgac cgagggtaga gaccagttcg tagtcattcc acggtttcga aatgaaacgg
     3001 atgatcccgg cctcgttgat ggcgtcgatc agtccgttca agtcggcgta gccggacagg
     3061 atcaggcgcg ccgcatcggg ttgtatttcg cgcaaggcct tgaggaactg cacgccattc
     3121 atgaccggca tgcgaaaatc ggagagcacc agatcgtagg ctgtgtgacg ggccttttcc
     3181 aaagcctctt tcggcgaact gcaaatgtcg accgtcagct tgtaggttac ctcgcccgcc
     3241 gtacagggcg tcagcgacaa caggcggcgc agggatttga ggatggattc ttcatcatcg
     3301 acaatcagga ttcggctcat cgcgtttttc cttcagtgtt tgtcagtgcg gatataaatc
     3361 gtaatcgcat ggttttcgcg ccgtgcatac tcttgaattt tcttgatgac ggcaacgtcg
     3421 aggacaaaat ccgctgccag cagcatgatg ccttccttgc tcagcagatc gcgcgccagt
     3481 accatgccga cctttagatc agcgtgggaa accgttttcg cgcggtcgct ttcaagcgcc
     3541 agaccaccca gcatctcgac gaaagcatcg acgatttgcg gatcgtaacg tttgccactg
     3601 gactggatga tcaaggcttt tgcctcatca gggctcatgc gcttatcgga tagcgtgccg
     3661 atctgcaagc catcgtagtc gttaaccacg ctgagtatcc gggcaccacg cacgatgtcc
     3721 gcgccctcaa gtccgtccgg aaagcccttc ccgtcaaatt gttcgtggtg cgcgcggaca
     3781 atccgggaga cgtctttcaa ttcctccagc ggcatcaacg ccgattcccc ggcgacaacg
     3841 tgcttgcgat actgcccgag ttcctcgctg cccatcttgg aaacggctcg aatgagcagg
     3901 ctatccggga atccgatctt gccgacatcg tgcaacagtg cggcgaaaaa aatgtcttga
     3961 agttccttgg attccacgcc cagacgaatc gccagcttac gtgccagatc ggcaacccga
     4021 cgcgagtgac ccgccatcgc gccgccacgc agttccatga gactggaaaa tatcttgatc
     4081 gaaacgagaa aattctgttt gagcttgtcg ttggccaggt tgaggaagct attgacctgc
     4141 tcgatttcgg atgtgcgctg cctgaccttg tcttcgagat tgaggttgag atccttgagc
     4201 tcctcgttct gcgcctgagt caatgccagc aggcgaatgt tttcgttgcg tagccgggcg
     4261 ccttccagtg cgtcgcggac caccaacttc aggtcgtcat cgtcccaggg tttggagacg
     4321 taccgataga tctcgccccg gttgatcgca gcgatggtcg aagcgacgtc ggcgtagccg
     4381 gtaagcagaa ttcgtgttgt cttcggccag cgattgcgta cctgctccag gaattgtgcg
     4441 ccatccatct ccggcatgcg catgtcggag atgaccagat caacgggttc atgttcaaga
     4501 acttcaagcc cggccttgcc gctttctgcc gtgaagatcc gatagccggc aggtcgcaac
     4561 agtcgtcgca acgaagaaag aatgcccggc tcatcatcga cgagcaaaac cgtcgtggcc
     4621 gtgtcgaggg tgtcgttcat aacgcggtct cgatattggc tgtcggtgac gaatctactg
     4681 atagcccctt gcccaaacct ttcatcgtta ttgaccctct cccaggtttt tccgactacg
     4741 ttgcctctga taatactccc tgacttcagc ttagaaccat tgtgggtatt atggggcaaa
     4801 accctgcgcg attgactcgt cgatacctag catatacatt actgaccagc cagtcattac
     4861 cggatttcga tgaatacact tgccactcac tctgttccgc ttgccaatgc cgaaacgcgc
     4921 gtaccacgtc gtcgtcgcaa ggatgcacgt ccctcggagt tgactgcggc tgcgcttgaa
     4981 ctcttcgtcg aaagaggctt cgccaccacg cggcttgaag atgtggcggc acgcgccgga
     5041 gtttccaagg gaaccttgta cctctacttt gacagcaagg aggctctctt caaggcggtg
     5101 attgaggaag gcatcgtgcc gacgctggtg gctgcagagc aacaattggc tgagcacagc
     5161 ggatcagcgg ctgaattgct gcgcaagctg cttttcggct ggtggcagca aatcggtggc
     5221 acacgtctgg ccggggtgcc aaagctgatc atttcggagt cgcgcaattt tcccgaagtg
     5281 gcgcagtatt accacgacaa ggtgatactg cgcggacggg ctttactccg taccgtgctg
     5341 cagcgaggaa tcgcggacgg cgagttccgg gctctggacg ttgaaaccgc catcgacgtg
     5401 attattgcgc ccttgctgat gctggtcatc tggcgttttt ctctttgttt ttgcgggcag
     5461 gagaccgatc cggagagcta tcttgagact cattttgatc tgctggtgca cggcttgcgt
     5521 cgatcgcaag atggccaatg aacgtatccg cagtccgcgg cagcatatgg cattgcccca
     5581 tctcccgggc agtgtggtat cgggtaaagg tataatcctt ggttcttgta ctaattcata
     5641 ttagactgtg ctaatatact ttttgaaatg gggaatgcca tggcggtagc ggcaatgaat
     5701 atcgagaaag cgcgtttcaa catgattgag cagcagatcc ggccctggaa tgtgctggat
     5761 ccgtcggttc tgagcctgtt atcagtcgtc aaacgcgagg atttcgtccc ggcggctcac
     5821 aaggatctgg cgttcgccga tattgaaata cccatcggcg ccaagggaaa tgccagccag
     5881 acgatgctgg cgccaaagat cgaggcgcgt atgctgcagg agctggcgat caggaatacg
     5941 gacaaagtgc ttgagattgg taccggcacc ggctatatgg ctgccctgct tgcggccaag
     6001 gctgaatatg tctacagcgt cgaaatcgat ccggggttgg ccgagattgc ccgcaataac
     6061 ctccagcagg ccggtgttgc caacgtcagt gtcgaaatcg gagacggcgt acaaggtcgg
     6121 tcaagtgatg cgccttatga tgtgatcgtg ctttccgggt cgctgccggt gttgcccgag
     6181 cggcttctca atcagctcaa gatcggtggg cgactggcgg ccgttgtcgg tgaagcgccg
     6241 gtaatgcagt tacaggtgat tacgcgcacc agtgaagacg cttttagcac gaaccctatt
     6301 tttgaaaccg ttgtcgcacc tttaaccaat gctgctcaga acaatagatt tgttttctag
     6361 cttatccatg agaccaagtc catgcgcctg aagctatcgc caagccgttc gtctctattg
     6421 cttgcagcgc tcttctctgc agcgccggcg ttttcgacag atctgatgca ggtttatcgg
     6481 gatgcactga ccaatgacca gcagtacatg gcggcacgtg caacggccga cgcattgcgt
     6541 gagaaagagc cgcaaggatt gtccggactg ttgccaacga ttagcggtac ggccaatacg
     6601 acctggaatg agaacaagta ttcggcgagc aaccagcctc agaaaatcaa cagggatttc
     6661 aataccaacg cttataacgt caatctgacg cagccgcttt tccgctggca aaacatcgtg
     6721 caatacagcc agggaaaact gcaggtcgtg caggctgagg caaattttgc tcaagcctct
     6781 caggatctga ttctgcgcgt tgcccaagcc tacttcgatg tgctctatgc ggaagagaac
     6841 ctcaaggcgg ttcgtgccaa caagcagtcg atcgcgcagc agctcgaaca ggccaagaag
     6901 aattttgagg tcggaaccgc caccatcacg gattcccagg aagcacagtc gcgtttcgat
     6961 ctggcgagcg cgcaggaaat cgctgctgaa aacgatctgg aggtaaagcg ttttgccctg
     7021 agcgtaatta tcggcaagca tccgggagag ctcaatcgcc tcaagccgca agccgaatta
     7081 aaaccgccgc aaccggccag catggatcca tgggttgatg cggccgagaa ggacagcttt
     7141 gtcgttcagg cccagcaggc cgctgccgaa gttgccgcaa aagaggttga gcgtaatcgc
     7201 gccgggcatt atccgaccct ggacgtcgtg gccaattacg gcaagaataa ttccgcgctc
     7261 tcgtttttag gcaacgatag tcttgaaacg acggcaagaa atattggttt gcagctcaat
     7321 attccgcttt tccagggcgg ttatgtcaat tccaagacac gggaagccgc ggccaatcgc
     7381 agcgcagcgc aagcgacact ggaaaacacc aaacgtactg cggcgcttaa cgcccgacaa
     7441 tcgtatctgg gcgtggtcaa tggtctggct caggtcaggg cgctcaacgc cgcgctgatt
     7501 tcaagtacca gtgcgctcga atcgaacaag ctcggttacg aggttggcgt acgcatcaac
     7561 atcgatgtgc ttaacgccga gaatcaggtt tacatcacaa agcgcgatct ggccagagca
     7621 cgtttcgaca ccctgctctc ccagctcaga ctcaaggcgg cagtgggttc gctcggcgaa
     7681 gccgatctgg aacagatcaa ttcgctgttc gaggcgcctt aatttcgcgt atcaacgcga
     7741 cggtcctgga cgtcgctccg cgatgcgcct gaccgaagcc tgcggccgca tcgcgcatcg
     7801 cttgcagcgc agcacgatca ttcaatagcc ttagcacttt gttggcagcg tcgtcggcat
     7861 cggcaattct catcgccgca ccgcaggcaa ttgcgtcgtc agttgcctgc gaaaagttga
     7921 agctgtgcgg accgaccagt gcagggcaat cgcaagcggc ggcttcgatc aggttctgtc
     7981 cgccgaaagg taacaaggtt ccaccaatca gcgccacatc cgccacggta aaataagcgg
     8041 gcatttcgcc catgctgtcg ccgagccaga cccgtgtttc ggaaacgggc agtgagttct
     8101 cgctgcgccg acagagcgta agtccgcgtt cttcgatcag agtacgcact tcgcgaaagc
     8161 gctgcgggtg gcgcggtacc agcaacagaa gcagatccgg ccgattcagg cggacgaagg
     8221 cgtccactat gagcgattcc tcgccctcgc gtgtgctggc agccagccag acaggccgcg
     8281 cgccgagttt ctgccgccac tggcttccca gttgcagctt cgccggcgat ggaatgacgt
     8341 caaactttag gttgccaacc acgctgacgc gctgcgcgcc gatggacatc agccgctctg
     8401 catcggccgg cgtctgtgct gccacggcac gcaaggagtc aaacgccggg cggaagagat
     8461 tcaacaagcg cctatagccg cgctgcgaac gagcggagag ccgggcgtta accagcaaca
     8521 gcggcatgcg gcgtgccttg gcgccagcaa tcagattggg ccacagttcg gtttccatta
     8581 gaagaccgag gcacggcttg aagtggtcaa ggaaacgccc gctggcgccg ggcaggtcat
     8641 agggaagata ggcctggatc agccgggagc cgtactttgc gatcagttcg gcgcctgtcg
     8701 ctcttccggt tggcgtcatg tgtgttaaca gcaaagagtg atccgtgtat tcgtcgagaa
     8761 gtgcctggat cagcggttcg gccgcgcgcg tctctcccac cgaaaccgca tgcagccata
     8821 acagtcgggc cggcgcaggc tggacgtagt ggccgtaacg ttcgccaagg ttttgaagat
     8881 actcgggctg cttgcgtgcg cgccacaaca gtcgcagcca gataagcggt tggatcaggt
     8941 aaaagagcag ggaatagact tggcgctcca tcaacgaatc cagcgcgcgc cctgttccgc
     9001 cacggtcagc acttcagacg ccgtcggtgc ctgaccctcg gcgccaagat tgcggatgaa
     9061 acccgagccg tagactccgg taagcgccgg atcgctggcg acgaatactg caatggttgg
     9121 cacgcgcagc gctgttgcga gatggaccag gccggtatcc acgccaatgg caagagcgga
     9181 accgccgatg agacaggcca gctcattcag cttcaagggt ggagcgacga tcgcacccgg
     9241 tacgttgctg acaatgcgct ccacacgttg acgctcaatc gcattgcccg aagggaacac
     9301 cggagtcagc ccgcgcttga acaggttttg agcaatagtc ttccagttcg tttcgtccca
     9361 tagtttgtcg tcgcggctgg tcgctgtgag caacaccgcg tagcgactgc cgacaaacca
     9421 cgaaagattg acggcggggg tgctgatgcc atagttgagt ggcaggtcga caggaatctc
     9481 gaaagccgca gcagcgagcc agcgattgcg ttcaacggcg tgggcattgg gcgggatgac
     9541 gaaggtttca tcgtagaaga gggccgctag cgattcgcgc gccacttcgg ccgcataccc
     9601 gcagtgccgg cctttggcct gtctggcaat cagcgcactc ttgatgagac cctgtgtatc
     9661 gaggatgacg tcgtagtatg tttggccaag gcgctttctg aacttgccaa tttcgcgcca
     9721 tgtagcccac tgagtcaggg tttttcgcca gcgtcgcagg gccaccggaa cgatttcgct
     9781 gacctcgggg tgcaggcgcg gaatgtcgga aaacgcctct tcgacgcacc agtcgatctg
     9841 cgcgtttgga aaacaacgcc gcaaatcgct gaccacaggc agattatgta tgacgtcgcc
     9901 tagcgaggag gttttgacca acaatatgcg catcggcagg tagaatagtg acgacttgaa
     9961 ggcagcatta taagagatag tgcaaattgc atcggaatat accctgccac taaaaacccg
    10021 aaaggaaatg acttgatctt ggtaaccggc ggcgcgggct tcattggagc caacttcgtt
    10081 cttgactggt tgcgcgagag cgatgaactc gtcctcaacc tcgacaagct gacctacgct
    10141 ggaaatctcg agaattttgg cgaactggtt ggtgatccgc gctacgcctt tgtgcacggc
    10201 gacattggtg atcagaaact ggttgagggc ttgcttctga agcaccgccc gcgcgccgtg
    10261 atcaatttcg cagcggagag ccatgtcgac cgatcgatcc acggcccagg ggagttcatc
    10321 gagaccaata tcgttggcac cttcaagcta ctcgaaagcg tgcgcgccca tttttcagcg
    10381 atgaatgatt ctgagagggt ggcgttccgc ttcctgcacg tttcgacgga cgaggtttac
    10441 ggctcgcttt ccgctgccga cccggccttc acggaaagca accgttacca gccgaacagc
    10501 ccctactcgg cgtccaaggc cgcatccgat catctggtgc gcgcctacca ccatacctat
    10561 ggcctaccgg tgctgacgac caattgctcg aacaactacg gtccgtacca gtttccggag
    10621 aagctgatcc cgctgatgat cgtcaatgcg ctggccggtc agccgctacc ggtctatggt
    10681 gacggacagc agattcgcga ttggttgtac gtaactgatc attgcgacgc catccggcat
    10741 gttctcgcat ccgggcgaat tggcgagatc tacaatatcg gcggctggaa cgagaaaccg
    10801 aatctcgcca ttgttcatgc gatttgtgac ttgctagacg agctgcgccc gcgtgccgac
    10861 ggagggcgct acagcgaaca gattacctac gttgcggacc ggccgggtca tgatcgccgc
    10921 tacgccattg atgccggcaa gatcgggcaa gaaatcggat ggaggccacg cgaaactttc
    10981 gctacgggga ttcgcaagac cgtacagtgg tatcttgatc atcagggctg ggtgagcaac
    11041 gtgcaatcgg gtgcctatcg cgaatgggtc gagaagaact acggaggaag agtcgaatga
    11101 agattctgct gtttggaaaa cgtggccaag tcggctggga attgcagcgt agcctggcgc
    11161 ctttggggca ggtgattgcc atcgaccttg atcatgttgg tgctgacggc ttgtgcggcg
    11221 actttacgaa tcggcctgga cttgccggaa cggtgcgccg agtcaaaccg gacgtcattg
    11281 tgaacgccgc cgcctatacc gccgtcgaca aggccgagtc cgaccacgac ctggcgcaca
    11341 ggatcaatgc tactgcccca ggcgtgctgg cggatgaggc tcaggagatt ggcgcctggc
    11401 tggtgcacta ttcgaccgac tatgtgttcg atgggcgagg gactacaccc tggctggaaa
    11461 ccgatgccac ccgaccgctc agcgtttacg gacaaaccaa actggagggc gagactgctg
    11521 ttgcgcgttg cgacaagcat ctcgtcttcc gtaccagttg ggtgtatgcg gcgcgcggtc
    11581 agaatttcgc caggaccatg ctgcgattgg cgcaagagcg tgatcagctc aaggttatcg
    11641 acgaccagat cggtgcaccg accggcgccg agctattagc cgatgtgacg gctcacgccc
    11701 tgcggacagt tgagaatcgc actgaactgg ccgggatcta tcatctggcc gcgggtggcg
    11761 agacaagctg gcacggctat gcaaaattcg tgattgaaca agcccgccac gcggggcaac
    11821 aggttcgggt tgcaccggaa gccattgagg ccattctgac cagcgactac ccgacaccag
    11881 ccgaaagacc gctcaactcg cgactcgata cccgcaagtt acaggcaaca tttggattga
    11941 tcctgcccga atggaagacc ggcgttgcgc ggatgctgac tgaggttatt gggcaagcgt
    12001 aggattggat cacggaactc ggccctcaat accctggttc taggcgaatt ccgcgctggc
    12061 actcattgcc accgatccgt ccggcccatg cgcttccagc gacacgcggt cgtcgtccgg
    12121 tatgccgacc aagcggaacg gcgccaggtc gaaaagggga gctaaaccgc gaaagctgaa
    12181 cttgctcacc ggccgcgccg catgatggcg caccagttcc atcagcagaa cggcggtcag
    12241 cggcccgtgt acgaccagac ccggataacc ctcttcgctg gtcgcgtagg ggcggtcata
    12301 gtgaatccgg tgggcgttga aggttagtgc tgaaaagcgg aacagcaatc tcgggtcggg
    12361 cgttactatt ttcgtccagg cattttccgc agcgggtgac cacgcgatga cctgcgggtt
    12421 ggcaactgcg gccccgggtt cccggtagac gatgtcctgc tcttcctcaa ggcacagcac
    12481 atcattctgg taataactgc aaacgaccga gacgaaagcc agcgagcctg agcggcctga
    12541 cttctgcttg atgtcgcgaa tcaccgcttc gcggcgagcg ggctgaccga tgatcaaggg
//
LOCUS       DAORPM010000037        12569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1053_length_12569_cov_10.118827, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000037 DAORPM010000000
VERSION     DAORPM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 12569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12569
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1053_length_12569_cov_10.118827"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLD53_03140"
     CDS             complement(<1..434)
                     /locus_tag="PLD53_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRC59931.1"
                     /translation="MPSVPLASKSPLPGVRVSKLMAERGLCSRREADVYIERGWVFVD
                     GERVSELGTRADPSANIKLDKGARAVQMQQISILLHKPVGYVSGQPEPGFTPAVTLVL
                     PENQYRTPDTPLFHASHLKGLAPAGRLDIDSTGLLVLTQNGRL"
     gene            complement(684..1028)
                     /locus_tag="PLD53_03145"
     CDS             complement(684..1028)
                     /locus_tag="PLD53_03145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC59932.1"
                     /translation="MSITTTESGLQIEEIRVGEGESATSGKFVSVHYTGWLTNGTKFD
                     SSKDRDEPFEFPLGQRNVIAGWDEGVQGMCIGGVRKLTIPSQLGYGARGAGGVIPPNA
                     TLVFEIELLDIL"
     gene            1196..2305
                     /locus_tag="PLD53_03150"
     CDS             1196..2305
                     /locus_tag="PLD53_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRC59933.1"
                     /translation="MDIHSIIAPVITDKLALEEFIESLGDLAPKIERDIARLKSTPGD
                     REVISSLFRSIHNIKGDAALCKVELAVTIVHPIETVLARFRNDEIAFTGIIAEAVLLA
                     LDRLELAAESLLSGRSLDGLHLLDLVQGLEKLSLETASNMDECACELIEAVTGFRPAS
                     EAAALLQGKASSISRKSKSQAADDLRFFRTLANQLEARSTLFKGRTMRLLRLAMETNQ
                     IMDKAVDPMQLEAAIYMHDIGMMFLPESVWLKQERMTDDEKLILRAHPDYADGILSRM
                     EGWESAAEMVAQHHEMPDGEGYPMGLKAKQICDGAKILAIVDAFEAVMLKHINRGKNR
                     SVLRAIAEINACNNQFAPDWITPFNQVIRRTLEAR"
     gene            2302..3081
                     /gene="tcdA"
                     /locus_tag="PLD53_03155"
     CDS             2302..3081
                     /gene="tcdA"
                     /locus_tag="PLD53_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HRC59934.1"
                     /translation="MIDPSADLERRFGGVRKLYGADALARFQAAHVCVVGIGGVGSWA
                     AEALARSGIGQITLIDLDMVAESNVNRQIHALGDEFGKAKTEAMAERILAINPACRVN
                     RIEDFVSPENLGQMLDRDFDYVIDAIDQVRSKTAMIAWCKSRGLPIITAGGAGGQVDP
                     TRIEVADLARTIQDPLLSKVRSLLRREYGFTRDQKKGFGIPAVFSSEPLRYPENGAAC
                     DEPPQMAGLNCAGFGSSVCVTAPFGLFAAAEVLKFLAAGAR"
     gene            3236..4210
                     /locus_tag="PLD53_03160"
     CDS             3236..4210
                     /locus_tag="PLD53_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRC59935.1"
                     /translation="MKKITGLIRTVLMTLICVCSIGSVTPANAQQYLRIGTGGTAGTY
                     YPIGTLIAGIVSQPGKILATAQASNGSLGNVIAVTSGALETGFSQADVATWAKNGTGI
                     FEGRPSLASLRLIANLYPESLHIVVRKDSGIKTVADLKGKRVALDEPGSGTLISARQI
                     LSAYGLKEADIRPEYIKPNQAGDKLIAGSLDAFFFTGGTPAAAIADLASAGAGIALLP
                     IDGPRAERLLKDNPFLHGDTIPAGTYQGVAAIQTLAVGAQWVTSEKIDSDLIYQVTRT
                     LYGEAGQKALASGHPKGRFITLGNAVKGAGIPFHPGAERYFREVGVLK"
     gene            4212..6830
                     /locus_tag="PLD53_03165"
     CDS             4212..6830
                     /locus_tag="PLD53_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC59936.1"
                     /translation="MRTVPIFGPSLLAILTGLLVTAFVGYSLVRERDVALEVASLETE
                     NFARVLEEHTRQSLRRINTGIVEANAALLESRDAGIRDPAKIAERLRGLLPPDRIIRS
                     FLVLDRTGTIVLSTQAIEAGRAGSRSDRDYFIPHIRGADRELVFGAPEPGSGDNKWIL
                     PISRRISTADGEFAGVLVAMVHGAHFQPFYDSIDSRPDGFVTLFLSTGWAAVSSPSDP
                     TVVGRNWSDTPLFRMHIPNWPTGTVREVLFGAGIERIYSYRALNDYPVVVSYGLAIPT
                     ILGPWLQIAWRDGFLLLVVLAALSGIATMLIRHERRRRKTESDLRIAATAFEAQQGVI
                     VTDADTVILRVNRAFTEDTGYSAEEAVGKTPRMLSSGRQDQRFYADMWRTLNTTGSWH
                     GELWNRRKNGEEYPERLSITAVKDSDGRLTHYVATLVDVTREKAAALEIEQLAFFDPL
                     TRLPNRRLLMDRLRHALASSARHGLHGALLFLDLDHFKTLNDTQGHDVGDALLAQVAQ
                     RLVQALREGDTVARLGGDEFVVLLEGLGERSLEAAELVHVVCEKILTTLNEPYMLNAC
                     QHHSSASIGATLFSGQQKTIDELMKQSDLAMYAAKTAGRNTVRFFDPNMQATITAHAL
                     LEDDLRRALSERQFRLHFQKQVTHDGRVVGAEVLIRWLHPGRGLIPPVEFIPHAEESG
                     LIEPIGQWVLETACEQLKRWEGNLRFASLRLAVNVSARQFHQSDFVQQVRDVLKRTGA
                     QPNLLKLELTESAVLTNVEETINKMNLLKTDGVQFSMDDFGTGQSSLSYLTRLPLDQL
                     KIDQSFVRNIGIRREDALIAQTIIGMANNLGIEVIAEGVETESQRAFLEQHDCPLCQG
                     YLFGHPMPIEEFERLF"
     gene            complement(6827..7771)
                     /locus_tag="PLD53_03170"
     CDS             complement(6827..7771)
                     /locus_tag="PLD53_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC59937.1"
                     /translation="MGQLISYEHGIHAFDAGYVRPLLASVHLVVENGRAAFVDTGTNK
                     SLPAAMAALDALGLDADSVDYVILTHIHLDHAGGAGAMMRQFPRARLVVHPRGARHMA
                     DPSRLVAGANAVYGADEVSRLYGDVLPIDQTRIIEATHGLKVDLAGREFLCLDTPGHA
                     RHHNCIVDRRSGHIFTGDTFGLSYRELDTDGRQFIFPTSTPSQFEPQALKESIDLLMT
                     YRPQAMYLTHFSQVKDVAAKARDLHRLIDAYLDIARREKNAGPERQPRIRNGLAQLLI
                     DETTRFGCRLPHAEILEIFAMDLELNAQGLVVWLDGQR"
     gene            complement(7877..8428)
                     /gene="msrA"
                     /locus_tag="PLD53_03175"
     CDS             complement(7877..8428)
                     /gene="msrA"
                     /locus_tag="PLD53_03175"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRC59938.1"
                     /translation="MTEYHLEYATLGGGCFWCLEAAFDQVVGVKSVVSGYCGGHTDNP
                     DYHAVCGGDSGHAEVVLITYDPAEIGYRDVLEIFFAIHDPTTLNRQGNDVGTQYRSVI
                     YYHSPEQSAVAESLLAELGASGLWSAPIVTQLLPVPRFYPAEDYHQRYFAANPHQPYC
                     QAVVSPKLAKFRKKFASRLKSAR"
     gene            8640..9578
                     /locus_tag="PLD53_03180"
     CDS             8640..9578
                     /locus_tag="PLD53_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRC59939.1"
                     /translation="MEIIVDVILKAGRSAVELSLFILLPVMVVMLSLMRLLEAKGVLD
                     WLVAKLAPLLKPFGLTGLGVFAALQINFVSFAAPIATLTMMDQRGASGRHIAATLAMV
                     MAMAQANVVFPMLAMGLQFGRVLFFSLLGGLVAASATYYFFGRNLSAEEKLIDETLHH
                     PVAENAKGVLDVINRAGAEAFKIAVGAIPMLVLSLTVIMALRAGGAIDALTQLMSPLM
                     DSLQLDRELILPTLTKFLAGGTAMMGVMDEMLRKEQVSELLLNQSAGFLIHPLDIPGI
                     AVLISAGRRVAGVWRPAALGACVGIFVRTLGHAYLG"
     gene            complement(9667..10872)
                     /locus_tag="PLD53_03185"
     CDS             complement(9667..10872)
                     /locus_tag="PLD53_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC59940.1"
                     /translation="MIRPVDSLHLALRAITAHRLRSFLTLLGIAVGIAAVILLTSIGE
                     GIHRFVLTEFTQFGTNLVSVVPGKVKTSGPAPTGIPTSVRPLTLEDAKALRRLPHITG
                     MSAVVWGNTEVKGNGRLRRTTVQGVTPDMLKVYSMKIASGQFLPPDETESARNFVVLG
                     SKLKSELFGVDNALGERLTVGNLQFRVIGVLESKGQFLGIDLDDAAYIPTVRALELYN
                     RDGMMTIDLSYEEGIPAARISASIVKLLTARHGREDFTITTQEDMLKTLSNILDILTM
                     AVGALGSISLLVGGVGIVTIMTIAVTERTGEIGLLVALGATRITILGLFLGEAVALSA
                     LGGVFGLALGFSLAQLIHLLVPALPVHTPIVFVIFAEIIAIAIGLLAGVLPARRAARL
                     DPVEALRAE"
     gene            complement(11007..12215)
                     /locus_tag="PLD53_03190"
     CDS             complement(11007..12215)
                     /locus_tag="PLD53_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC59941.1"
                     /translation="MRTPDLIRFARDAATGNPLRSSLLVLAMAIGVAAVVVLTALGDG
                     ARRYVVNEFSSLGTNLVIVLPGRSQTGGFSPGNAITSTVRDLTIDDAQALLRASAVRR
                     VAPLAVGTSEISFGGKLREVVLAGTNAQFIDVRNLKLAQGRFLPEGDWRRGASEAVIG
                     AKIRDEMFGNESALGQLIRIGDRRFRVVGVLASTGQGLGMNTDELVIVPVSLAQAMFN
                     SNTLFRILVEANSREAIESAKTQVTAIIKQRHEGEEDVTIITQDAVLATFDKLLGTLT
                     LAVAGIAAISLAVAGILVMNVMLVSVTQRTSEIGLLKALGATGATIRSAFLTEAAMLS
                     VAGALLGYLLGQGAAALIRLLYPVFPAFPPDWAVLAGLGTALFTGIVFGVLPARQAAR
                     LDPVQSLAKR"
     gene            complement(12336..>12569)
                     /locus_tag="PLD53_03195"
     CDS             complement(12336..>12569)
                     /locus_tag="PLD53_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HRC59942.1"
                     /translation="RATIMQPAMILADEPTGNLDRATGEEVMRLLEGLNDHGVTLIVV
                     THDKELGARARRQLLMEDGEVRHDSACKEESNA"
BASE COUNT         2584 a         3762 c         3781 g         2442 t
ORIGIN      
        1 aggcgaccgt tctgcgtcag caccagcagg ccggtcgaat cgatatccag ccgcccggcc
       61 ggcgccagac ctttcaagtg cgaagcatgg aagaggggcg tatcgggagt gcggtactga
      121 ttctccggca gcaccagcgt aacggcagga gtgaagcccg gttccggctg cccggaaaca
      181 tagccgaccg gcttgtgcag cagaatgctg atctgttgca tctgtaccgc gcgtgcgccc
      241 ttgtccagct tgatgttcgc cgatggatcg gcacgtgtcc ccagctcgga aacgcgctcg
      301 ccatcaacga aaacccaacc ccgctcaata tagacatccg cctcccgccg cgaacacaga
      361 ccacgctcgg ccatcagttt ggagacgcgc acgccaggca gcggtgactt gctcgcaagg
      421 ggtacggaag gcacggaacc cggacctggg ccgtgaccaa ggggctttgc aataactggc
      481 gcacgtgacg gcgttcctcg cggcttctgc tgcggtgacg ccacgtcagc cggaaatgga
      541 ctgttcgcaa ctccgccgtc cgacctgttt ctcagccttg gcgcccccct gcgcagtggt
      601 ttgcgccctg gtccgatcgg tgtctcgctt cccgtgctcc ttgcaggcag gccgagcggc
      661 ttgcgcgggg tatcggtcat gggtcagagg atatcgagca attcgatttc gaataccagt
      721 gtcgcatttg gcggaatcac gcctcctgca ccacgagccc catagccgag ctgcgatgga
      781 atcgtgagct tgcgaacacc accgatgcac atgccctgca caccttcatc ccagccggca
      841 atcacgttgc gctggccaag cggaaattca aagggctcgt cgcgatcctt gctcgaatcg
      901 aatttggtgc cgttggtcag ccagccggtg tagtgaacgc tcacaaactt gccggaagtg
      961 gcgctctcac cctcgcctac ccttatttcc tcaatttgca gaccggattc tgttgttgta
     1021 atggacatga tctcacctcc tgtgtttgaa ctacggagtg tagaccagct cggacaaagc
     1081 aaccagttca ttacgcactc ttaagccaaa caagatcaag ccaacacgac acgcgctaac
     1141 cctcgacccg ggtttcagat aaacttgctc caatcaagaa gccgggagaa gcatcgtgga
     1201 catccactcg atcattgcac cagtgatcac agacaaactt gcgctggaag aattcatcga
     1261 gtcactgggc gatctagctc cgaaaatcga gcgcgatatc gcccgcctga agagtacccc
     1321 aggtgaccgc gaagtcatca gcagcctttt tcgctcgatt cacaatatca aaggcgatgc
     1381 cgcgctgtgc aaggttgaac tggcggtgac catcgttcat ccgatcgaga cggtgcttgc
     1441 tcgattcagg aatgatgaga ttgcgttcac cggcatcatt gccgaagctg tattgctggc
     1501 attggaccgg ctggaactgg ccgctgagag cctgctttcc gggcgatccc tcgatggcct
     1561 gcatctgctc gatctggttc agggattgga aaagctttcg cttgaaacgg cgtccaacat
     1621 ggacgagtgc gcctgcgaat taattgaagc ggttacggga ttccgaccgg cctccgaagc
     1681 cgctgccttg ctgcaaggga aagcctcttc catttccagg aagtcgaaat cgcaggccgc
     1741 tgacgacctt cgtttcttcc gtacgctggc gaatcagctt gaggcgcgtt ccacgttgtt
     1801 caaaggacgc accatgcgtc tgttgcgcct ggccatggaa acgaatcaga taatggacaa
     1861 agcggttgac ccgatgcaac tggaagcggc catctacatg cacgacatag ggatgatgtt
     1921 tctgcccgag tcggtgtggc tgaaacagga gcgcatgacc gacgacgaaa agctgatcct
     1981 gcgagcgcac cccgactatg ccgacgggat cctgtcgcgc atggagggct gggaaagtgc
     2041 cgccgaaatg gtcgcccaac atcatgaaat gccggacggc gaggggtacc cgatgggtct
     2101 caaggcgaaa cagatttgcg acggcgcgaa aatactggcg atcgttgacg cctttgaggc
     2161 ggtcatgctg aagcacatca accgcggcaa gaatcgctcc gtattgcgcg ccattgccga
     2221 gatcaatgcc tgcaacaacc agttcgctcc cgactggatc accccattca atcaggttat
     2281 ccggcggacg cttgaagccc gatgattgac ccgtcagcgg atctggaacg ccgcttcgga
     2341 ggagtgcgaa aactatacgg tgccgatgct ctcgcgcgtt ttcaggcggc acatgtctgc
     2401 gttgtcggaa ttggcggcgt tggctcgtgg gctgccgagg cattggcgcg ttccggaatt
     2461 ggccagatta cgctgatcga tctggacatg gtggccgaat cgaacgtcaa ccggcagatc
     2521 cacgccctcg gcgacgaatt tggcaaagcc aagacggagg caatggccga acgcattctg
     2581 gccatcaatc cggcgtgtcg ggtcaatcga atcgaggact tcgtatcccc ggagaatctc
     2641 ggtcagatgc tcgataggga tttcgactac gtgatcgatg ccattgatca ggtgcgcagc
     2701 aagacggcga tgatcgcctg gtgcaaatct cgcggcctcc ccatcatcac cgcgggtggc
     2761 gccggtgggc aggtcgaccc aacgcgcatc gaagtcgccg atctggcgcg caccatacag
     2821 gatcctctgc tgtccaaagt acgctccctg ctgcgcaggg aatacggctt cacgcgcgat
     2881 cagaaaaagg ggttcggaat tccggctgtg ttttcgagcg agccgttgcg ctatcccgag
     2941 aacggtgcgg cctgcgatga accgccgcag atggccgggc tcaattgcgc cggcttcggc
     3001 tcgtcagtct gcgtcactgc ccctttcggc ctgtttgccg cggccgaagt actcaaattt
     3061 ctggcggctg gagcccggta acggaccgga accgatctgc gtcgatgttg acggaaagac
     3121 gggaaaatcc gactcgccgt tcgccagcgc attgcctcca tgccaattaa ctgagaaact
     3181 aaggctcaac ccagcggaca ccgggcctgc ccggaaagta ccttcaggag gtgccatgaa
     3241 aaagatcacg ggtctgattc ggacagtgtt gatgacactg atctgtgtct gcagcatcgg
     3301 cagtgtgact ccggcgaacg cgcagcagta tctgcgtatc ggcaccggcg gcacggcggg
     3361 aacctactat cccatcggta cgttgattgc cggcatcgtg tctcaaccgg ggaagattct
     3421 ggcgacggct caggccagca acggatccct cggcaacgtc attgccgtca ccagtggcgc
     3481 cctcgaaacg ggcttcagtc aggcggacgt agccacctgg gccaaaaacg ggacagggat
     3541 tttcgagggg cgccccagtc ttgccagcct gcgcctgata gccaacctgt acccggaaag
     3601 cctgcacatc gtcgtgcgca aggactcggg tatcaaaacg gtagcggatc tgaaaggcaa
     3661 gcgggtcgct cttgacgagc ccggatccgg cacgctcatc agcgcccggc aaattctctc
     3721 agcctacggt ttgaaggaag ccgacatccg cccggagtac atcaaaccca atcaggcggg
     3781 cgacaaattg atcgccgggt ctctggacgc cttcttcttt accggcggca cgcccgccgc
     3841 agccattgcg gatctggcat ccgccggagc cggcatcgcg ctgctgccga ttgacgggcc
     3901 acgcgccgaa cggttgctga aagacaaccc atttctgcat ggcgatacga ttccggcggg
     3961 gacatatcaa ggtgtcgccg caatccagac tctcgctgtc ggcgcacaat gggtcaccag
     4021 cgaaaagatc gattccgacc tcatctatca ggtcaccaga acgctatatg gtgaagccgg
     4081 gcaaaaggca ttggccagcg gccatccgaa aggaagattc attaccctcg gcaacgccgt
     4141 gaagggagcc ggcatcccgt ttcatccggg tgccgaacgc tattttcgtg aagtcggggt
     4201 gctgaaatag catgagaact gtgccgatat tcgggccttc gctactggcg atactgaccg
     4261 gcttactggt gacggcattc gttggttaca gcctggtccg ggagcgcgac gtcgcactcg
     4321 aggtggccag tctggaaacc gagaatttcg cccgcgtgct ggaagagcat acgcgtcaat
     4381 cactgcgacg aatcaacaca gggatcgtgg aagcaaatgc cgcattgctg gaatcgcgtg
     4441 acgccggcat acgcgatccg gcaaagattg ccgagcgcct gcgaggactg ctgccacctg
     4501 atcgaatcat tcgttccttt ctcgtgctcg atcgaacggg cacgatcgta ctctccacgc
     4561 aggccatcga agcagggcga gccggatccc ggtcagaccg cgactacttc attccgcaca
     4621 ttcggggcgc cgatcgtgag ttggtgtttg gtgcgccgga acctggcagc ggcgacaata
     4681 aatggatact cccgatcagc cggcgcatca gcacagccga tggcgaattt gccggcgtgc
     4741 tcgttgcaat ggtccacggc gcgcatttcc agccgttcta cgattccatc gacagtcgcc
     4801 cggatggctt cgtaacgctg tttctctcga caggctgggc tgccgtaagt tcgcccagcg
     4861 acccaaccgt ggtcggtcgc aactggtcgg atacgccgct gtttcggatg cacattccca
     4921 actggcctac cggtacggtt cgcgaagtac tgttcggcgc cggtatcgag cgcatctaca
     4981 gctaccgggc attgaatgac tatccggtgg tcgtcagcta cgggctggcg atcccgacca
     5041 ttctcggccc gtggctccaa atcgcctggc gcgatggttt tctgctgctg gtcgtactgg
     5101 cggcgctatc gggcatcgcc accatgctga ttcggcacga gcggcggcgc cgcaagacgg
     5161 aaagcgatct acgcatcgcc gcgaccgctt tcgaagcgca gcagggcgtc atcgtcaccg
     5221 acgccgacac ggtcattctt cgggtcaacc gcgccttcac cgaggacacc ggttacagtg
     5281 ccgaagaagc cgttgggaag actcccagga tgctcagttc ggggcgacaa gatcagcgat
     5341 tctatgctga catgtggcgc accctgaaca cgacaggctc ctggcacggc gaactgtgga
     5401 atcgacgcaa gaatggcgag gaataccctg aacgcctctc catcaccgca gtaaaggatt
     5461 ccgacggccg gttgacgcac tacgtggcca ccctggtcga cgtcacacgt gagaaggccg
     5521 cggcgcttga aatcgaacaa ctggcgttct ttgacccgct caccagactg cccaaccggc
     5581 gtttgttgat ggatcgactg cggcacgcgc ttgcgtcaag cgcccggcat ggcctgcacg
     5641 gcgccttgtt gttcctcgat cttgatcact tcaagacgct caacgacacc cagggccatg
     5701 atgtgggcga tgcccttctg gcgcaggtgg cacaacgact ggtccaggca ttgcgcgaag
     5761 gcgacacggt tgcacgactg ggtggtgacg agtttgttgt cctcctcgaa ggcttgggtg
     5821 aacgcagcct ggaggccgcc gagctggtgc atgtcgtctg tgaaaagatc ctgacgaccc
     5881 tgaacgaacc ctatatgttg aacgcatgcc agcatcacag cagcgcgagc attggcgcca
     5941 cccttttcag cggtcagcaa aagaccatcg acgagctgat gaagcagtcc gatctggcca
     6001 tgtatgcggc caaaaccgcc gggcgaaaca cagttcgctt ctttgacccg aacatgcaag
     6061 ccacaattac cgcccacgcg ttgctcgaag atgacctgcg tcgggcgctg agcgagcgac
     6121 agttccgcct gcattttcag aaacaggtca cgcacgacgg ccgggtcgtg ggagccgagg
     6181 tattgattcg ctggctgcat ccggggagag gcctgatccc gcccgtcgag ttcattccac
     6241 acgcggagga gtcgggcctc atcgagccga tagggcaatg ggtactggaa acggcctgcg
     6301 aacaactcaa gcgttgggaa ggaaacctgc gcttcgcttc acttcgattg gccgtcaatg
     6361 tcagcgcccg gcagttccat caatcggatt tcgtgcagca ggttcgcgac gttctgaaac
     6421 gcaccggagc gcaacccaat ctactcaagc tcgaattgac ggaaagcgcc gttctgacca
     6481 acgtcgagga aacgatcaac aagatgaact tgctgaagac cgatggcgtg cagttttcaa
     6541 tggacgactt cggaaccgga cagtcctcgc tctcctatct gacgcggctg ccactcgacc
     6601 agctcaagat cgaccagagt tttgtgcgta atatcggcat ccgtcgcgaa gacgccctga
     6661 tcgcccagac catcatcggc atggccaaca atctggggat tgaagtcatc gccgaaggcg
     6721 tggaaaccga atcgcagcgg gctttccttg agcagcatga ctgtccgcta tgtcagggct
     6781 acctgtttgg acatccaatg ccgatcgagg aatttgagcg cctgttctag cgctgcccgt
     6841 cgagccagac aacaagaccc tgggcgttaa gctcaaggtc catcgcgaaa atctccagga
     6901 tttcggcgtg tggcagccga cagccaaatc tcgtggtttc gtcgatcagc agttgcgcca
     6961 gtccgttacg gatacgaggc tgcctttcag ggccggcatt cttctcacgc cgggcaatgt
     7021 ccagataggc atcgatcagg cgatgcaaat cacgcgcttt tgcggcgacg tccttgacct
     7081 ggctgaagtg cgtcagatac atggcttgcg gccgataagt catgagcagg tcaatcgact
     7141 ccttgagagc ttgcggctcg aactggctgg gcgtcgacgt cgggaagatg aactgacggc
     7201 catcggtatc gagttcccga tacgacagcc cgaaagtgtc gccggtgaag atatgcccgc
     7261 tgcggcgatc aacgatgcag ttgtgatgtc ttgcatgacc cggcgtatcg agacaaagga
     7321 actcgcggcc ggccagatcg actttcagcc cgtgcgtcgc ctcgatgatt cgcgtttggt
     7381 cgatcggcaa cacgtcgcca tagagccggc tcacttcgtc cgccccgtat acagcgtttg
     7441 cgccagccac cagccgggat ggatcggcca tgtgccgcgc gccgcgtggg tgcaccacga
     7501 gtcgcgcacg cggaaactgg cgcatcatcg cgccggcacc accggcatga tcgagatgga
     7561 tatgcgtgag gatgacgtag tcgacgctgt cggcatcaag accgagtgcg tcaagtgccg
     7621 ccatcgcggc aggtagcgat ttgttggttc cggtatcgac aaaggccgcg cgaccgtttt
     7681 cgacgaccag atgaaccgac gccagtagcg ggcgaacgta gcccgcatcg aaggcatgaa
     7741 taccatgctc gtaactaatc aactgaccca tgaacaacgc tttcttttga ccaatcggcg
     7801 attatagttg cgatcgcccg gtgccggcca tcgcagacgg gcgaatcgca ttgatttgct
     7861 cgcggggcaa tgctgcttat cgagccgatt tgagacggct ggcgaacttt ttccggaact
     7921 tcgcaagttt cgggctgacg acagcctggc agtacggttg atgcgggttc gcggcaaaat
     7981 agcgctggtg gtagtcttca gcgggataaa accgtggcac cggcaacaac tgtgtaacga
     8041 tcggcgcgga ccagagcccg gaggcaccca actcggcaag cagggattca gcaacggccg
     8101 attgctccgg ggagtggtaa tagatcaccg aacgatactg ggtaccgacg tcgttgccct
     8161 gccgattgag cgtggtcgga tcgtgaatcg caaagaagat ttccagcacg tctcggtagc
     8221 cgatctcggc aggatcgtag gtgatgagca ccacttcggc gtgcccggaa tcgcctccgc
     8281 agacggcatg atagtcgggg ttgtcggtgt gtccgccaca atagcccgaa acgaccgact
     8341 tgactccgac gacctggtca aatgccgctt ccaggcacca gaagcagccg cccccaagtg
     8401 ttgcgtactc cagatggtat tcggtcatgg caattcccga tcaagacgta ctgctcagac
     8461 cagcaaatca gggaaaagct cccgatacaa tgcatgagta acgcgcaatg gacgactgaa
     8521 ctgtcgctaa cgcttccgaa aaccgtggtc cgcttcccta accatgccag ttagagcggc
     8581 aacggattcc tctcctctcc gttcgttaat ccagcggcga atcaaggata atccggtgca
     8641 tggaaattat cgtcgacgtc atcctcaaag ccggccgctc ggcagtcgag ctttcgctct
     8701 tcatcctgct gccggtcatg gttgtgatgc tctcgttgat gcgactgctt gaagccaagg
     8761 gagttctcga ctggctggtg gcaaagctcg ctccgctact caagccgttc gggctgaccg
     8821 gtctcggtgt ttttgccgcg ttgcagatca acttcgtgag cttcgctgcc ccaatcgcca
     8881 cgctgacgat gatggatcag cgcggcgcgt caggacgcca tatcgcggcg accttggcga
     8941 tggtaatggc catggcgcag gccaacgttg tttttccgat gcttgcaatg ggtctgcaat
     9001 ttgggcgtgt gctgttcttc tcgctgctcg gcggtctcgt tgccgcgtcg gcgacctact
     9061 acttttttgg ccgcaatctg tcagcggaag aaaagctgat cgacgagacg ctgcatcacc
     9121 cggttgccga gaacgccaaa ggcgtcctcg acgtgatcaa tcgtgccggt gccgaggcgt
     9181 tcaagattgc cgttggtgcc attccgatgc tggtcctgtc gctgacggtg atcatggcgc
     9241 tgcgcgcggg cggcgccatt gatgcgctga cccaactgat gtcaccgctg atggacagcc
     9301 tgcaactcga ccgtgaattg atcctgccga cgctcaccaa gtttctcgcc ggcggcacgg
     9361 cgatgatggg cgtcatggac gaaatgctgc gcaaggagca ggtcagcgaa ttgctgctca
     9421 accagagcgc cggcttcctg attcacccgc tcgacatccc ggggattgcc gtactgattt
     9481 cagccggccg tcgggtagcc ggagtctggc gtcctgccgc gctgggcgct tgcgtgggga
     9541 ttttcgtacg cacgctgggt catgcgtatt tgggttgatt ctgggctgat tcggggtgta
     9601 ccgattgacc cgatgcgatg aattcgaaga ttatctgatc gcgattgctt gctgaccgtc
     9661 ccgacctcac tcggctcgca gcgcctcgac cgggtcgagg cgggccgcac ggcgcgccgg
     9721 aagcacaccg gccagaaggc cgatggcgat ggcgatgatc tccgcgaaaa tgacgaagac
     9781 aatcggcgtg tgcaccggca gcgcgggcac cagcagatga atcagttgcg ccaggctgaa
     9841 gccgagcgcc agcccgaaga cgccgccgag cgcggatagc gccaccgcct cgccgagaaa
     9901 aagccctagg atcgtaatgc gcgtcgcgcc gagagcgacc agcaggccga tctcgccggt
     9961 gcgttcggta acggcgatgg tcatgatggt gacaatgcca acgccgccga ccagcagcga
    10021 aatgctgccc agcgcaccga ccgccatggt caggatgtcg agtatgttgg acagggtctt
    10081 caacatgtct tcctgcgtcg taatagtgaa gtcctcgcgc ccatgccgcg ccgtgagcag
    10141 cttgacgatc gacgcgctga tgcgcgccgc cggaatgcct tcttcgtagg acaggtcgat
    10201 ggtcatcatg ccgtcgcggt tgtagagctc gagcgcgcgt accgtcggga tgtaggcggc
    10261 gtcgtccagg tcgatgccga ggaactggcc ttttgattcg agcacaccga taacgcggaa
    10321 ctgcaggttg ccgaccgtca ggcgttcgcc cagcgcatta tcgacgccga acaactcgct
    10381 cttgagtttc gaaccgagca cgacgaagtt gcgtgcgctc tcggtttcgt cgggcggcag
    10441 aaactgcccg ctggcgatct tcatgctgta taccttcagc atgtcagggg tgacgccttg
    10501 gacggtcgtg cggcgcaagc ggccattgcc cttgacctcg gtattgcccc acaccacggc
    10561 cgacatgccg gtgatgtgcg gcagccggcg cagcgccttg gcgtcctcga gggttagcgg
    10621 gcgaaccgag gtcgggattc cggtcggcgc cgggcccgag gtcttgacct tgcccggcac
    10681 gacgctgacc aggttggtgc cgaactgcgt gaattcggtc agcacaaaac gatgaatgcc
    10741 ctcgccgatc gacgtcagca atatcacggc ggcgatgccg acggcgatgc ccagcagcgt
    10801 cagaaaactg cgcagccggt gcgcggtgat agcgcgcaag gcgagatgaa gggagtcgac
    10861 cggacggatc atgattgaat gaacgacgaa actgcgaacc accaagacac gaagacacaa
    10921 agaatcacca agaaagacaa aaatatttca agggttttct tggtgtaact tggtgacttg
    10981 gtggcttggt ggttaagcat ctgatctcac cgtttcgcca gcgactgcac cggatcaagc
    11041 cgtgccgcct gtcgtgccgg cagcacgccg aagacaatgc cggtgaacag cgccgtgccc
    11101 aatccggcaa gtaccgccca atcgggaggg aaggccggga agaccgggta gagcagacgg
    11161 atcagcgcgg cagcgccctg cccgagcaga tagccgagca gcgcaccagc caccgaaagc
    11221 atcgccgctt cggtcagaaa ggcgctgcga atcgtcgctc cggtggcgcc cagtgccttg
    11281 agcaggccga tctcggaggt tcgttgcgtc accgaaacga gcatcacgtt catcaccaga
    11341 atcccggcca cggccaggct gatcgccgca atgcccgcga cggcgagggt cagcgttccg
    11401 agcagtttgt cgaaggtggc cagcacggca tcctgcgtga tgatggtgac gtcttcctcg
    11461 ccttcgtgac gctgcttgat gatcgcggtg acctgcgtct tggccgattc aatcgcttcg
    11521 cggctgttcg cttcgaccag gatgcggaac agcgtattgc tgttgaacat ggcctgcgcc
    11581 agcgagaccg gaacgatcac cagctcgtcg gtgttcatgc ccagcccctg gccggtcgat
    11641 gccagcacac cgacgacgcg aaaacgccgg tcaccgatgc ggatcaactg gccgagtgcc
    11701 gattcgttgc cgaacatctc gtcgcggatc ttggcgccga tcaccgcttc cgacgcaccg
    11761 cgccgccagt cgccttcggg cagaaaacgc ccctgcgcca gcttcaggtt gcgcacgtcg
    11821 atgaactgtg cattggtgcc ggcaagcacg acttcgcgca gcttgccgcc aaagctgatc
    11881 tccgaagtgc cgaccgccag cggcgcgacg cggcgcacgg cgctggcgcg cagcaacgcc
    11941 tgcgcatcgt cgatggtcag gtcgcgcacc gtgctggtga tggcgttgcc cgggctaaag
    12001 ccgccggttt gcgaacggcc gggcaggacg atgacgaggt tggtgccgag cgaggaaaac
    12061 tcgttgacca cataacgccg cgcgccgtcg ccgagcgcgg tgagtaccac caccgcggcg
    12121 acgccgatgg ccatggccag aaccagcagc gaggagcgca acgggttgcc ggtcgccgcg
    12181 tcacgggcaa aacggatcag gtcaggggtg cgcatgtatt tgaaggggtg caacaaagcg
    12241 aaacaccaag ccaccaagag ctccaagtta caccaaggtc aatccataaa tcatgtcgtt
    12301 cttggtgcat ctctgtgttc ttggtgactt ggtggttaag cattcgattc ttccttacag
    12361 gcactatcgt gcctcacctc gccgtcttcc atcagcaact gcctgcgtgc gcgcgcgccg
    12421 agttccttgt cgtgggtcac gacgatcagc gtgacgccgt gatcgttgag tccttcgagc
    12481 aggcgcatga cttcttcgcc ggtggcgcgg tcaaggttgc ccgtgggctc gtcggcgaga
    12541 atcatcgccg gctgcatgat cgtcgcccg
//
LOCUS       DAORPM010000038        12374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1074_length_12374_cov_11.037909, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000038 DAORPM010000000
VERSION     DAORPM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 12374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12374
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1074_length_12374_cov_11.037909"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..2292)
                     /locus_tag="PLD53_03200"
     CDS             complement(79..2292)
                     /locus_tag="PLD53_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRC59943.1"
                     /translation="MLNSLLSPHHWYKLVAALACALLFTTPALADPPGRIGRIAWLSG
                     SGSGAVSLYNPETGESYSAPLNHPLTSGDVLTTGPGSQAEIQIGSMTVRLDSDTTLDF
                     SRIDDEQVRLYLSGGRAIAKLPSRDVAREFELNTPNGRFNANDAGIYRFDADANSSSA
                     TAYYGSLHFESKDSSLEIGAGQSAQFWQTGQGGDQTRYRLSTAINDDFTQWSAARDRR
                     PASTAYTRYVSPEMTGAEDLDAYGSWSQNTEYGAIWYPRAVAPDWAPYRNGHWAWVAP
                     WGWSWVAYEPWGFAPFHYGRWVHHRGAWGWVPGVRVARPAYSPAMVAWIGSPGIGISI
                     AIGTRPTVGWFPLAPREVYVPAYRSSPKYVRHINVTHVTHINNVTEIVNRPQHVVQKT
                     RYAHREQPRAVTFVPADVVTHRRPVQSAALSPRDHHALRNQPLQAAAPIPTPPPPQIE
                     SRSRHEPRDRSGPSVARQERERQTPVVANPTAPEMRDSQPRARSSARPSSEAVPLRSE
                     RGSPDNVRPTPESRLGSSEPRTQERAARREEPAMRQPQHDPRIEAAARVAPQENVRTV
                     PENARTETQSRPGSFEPRTPERPDRREETAMRQPQRESRIEAAPRVAPQENARAVPEN
                     TRTAPWSRTEPFESRAQERPNRREEPAMRSPQREARSEVSQQQVRQAPPVRPETPMPV
                     VHEPRHEVPRNAGGQASRSAEARAATNTQHQPESQRPGQNGREKQRHQRDDAEKR"
     gene            complement(2456..3181)
                     /locus_tag="PLD53_03205"
     CDS             complement(2456..3181)
                     /locus_tag="PLD53_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRC59944.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGAMFSKIAKEITVAAKMGGGDPG
                     FNPRLRLAVDKGKGVNMPKDKIDNAIKKGTGELEGVDYVEIRYEGYGISGAAVIVDCL
                     TDNRTRTVAEVRHAFNKHGGNMGTDGCVAFQFTHCGQLLFAPGTDEAALMDAAIEAGA
                     DDVLSNDDGSIEVITPPSEYMSVKEALEAAGFSAEFGQVTMKAENETELTGDDAVRMQ
                     KLIDVLESLDDVQDVYTNVILEE"
     gene            3334..4863
                     /locus_tag="PLD53_03210"
     CDS             3334..4863
                     /locus_tag="PLD53_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRC59945.1"
                     /translation="MAEPLIVARHKLTDLGLLPALANRHGLITGATGTGKTITLQVLA
                     ERFSAIGVPVFMADVKGDLSGLSAPGVESAKFKQRLDTLGISDWTGAKFPASFWDVSG
                     EQGHPVRATISDMGPILLSRLLNLNDTQSGVLQLVFKIADDNGLLLLDLKDLRAMVQH
                     VGENAKTFTTEYGNVSAASIGAIQRGLLTLGEQGGEAFFGEPMLDIADLMQTDGGKGI
                     VNILAADKLMSSPRLYSTFLLWLLSELFENLPEVGDADKPKLVFFFDEAHLLFNEAPP
                     ALLEKIELVVRLIRSKGVGVYFVTQNPLDIPDSVLGQLGNRVQHALRAFTPRDQKAVK
                     TAADTLRANPAFDAAAAITELGVGEALVSFLDEKGRPNVVERAFILPPASRIGPLTAE
                     ERQGVIKASLVYGVYETALDRESAYEKLKSKRTAEAAPTQPSAPAQVPANAPASGGGW
                     FGGLGDLIGGSSGSGGRSRGGDSMVEMAAKSAARAIGSQVGRSIIRGVLGSILGGSRR
                     R"
     gene            4991..5884
                     /locus_tag="PLD53_03215"
     CDS             4991..5884
                     /locus_tag="PLD53_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC59946.1"
                     /translation="MKRLSAERLGVVLAIFAALGFSFKAILIKLAYAAAPVDAVTLLS
                     IRMIFALPAFLWVGFVVSRSAPPLTRRDWGKLVALGLLGYYGASILDFIGLQYISAGL
                     ERLILFTYPTLTVMIGVLFMGKALEMREVISLLLSYCGIGLAFAHDLQVANDMATVLI
                     GGAFVFASSLSYAFYLSGSGPMIQRLGSARFTALAMLVSTLAAQLHFFAVQPISAFIQ
                     PLPVYAYCLAMALFSTVLPVFMQSAAIRRIGSSKAVLIGTLGPVLTIFFGWWLLGEPL
                     SLAQMGGAGLVLTGVWLVSRR"
     gene            complement(5933..6682)
                     /locus_tag="PLD53_03220"
     CDS             complement(5933..6682)
                     /locus_tag="PLD53_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRC59947.1"
                     /translation="MNDEQLLRYSRHILLDEIGIEGQAKIVGARALVIGAGGLGSPAA
                     LYLASAGVGRITLADGDRVDLTNLQRQILHTQERVGQFKAVSGRTALLQINPEVTVEV
                     ICERLANERLEALVYDADVVLDCCDNFATRYAVNRACVRHRTPLISGAAIRFSGQLSV
                     FDSRREDSPCYHCLFPESEDVQEERCAVMGVFAPLTGIIGAMQAAEALKVICGTGEPA
                     VGRLLLFDGLTMDWRCVKFGKDSKCAVCGTG"
     gene            complement(6755..7180)
                     /locus_tag="PLD53_03225"
     CDS             complement(6755..7180)
                     /locus_tag="PLD53_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59948.1"
                     /translation="MKRPAASPNKTPPIRKERNVLFSKFPPGQVPEASDFLSKIEHVE
                     VEPRVERRAVGVAYDLHQHSLQELEGALEDKGYHLDNTLMSKMMRALIYYVEETQLHN
                     LDAPERPLKKSQHEAYTHAWERHPHGDHDDTPPEWREYK"
     gene            complement(7201..8613)
                     /locus_tag="PLD53_03230"
     CDS             complement(7201..8613)
                     /locus_tag="PLD53_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRC59949.1"
                     /translation="MGKLKQAGLICAGILGGILISLQFSAMAEKETPTSLPIEELRTF
                     AGVFNAIKQGYVEPVDDKKLITYAISGMLSNLDPHSTYLDADAYKDLQVGTQGEFGGL
                     GIEVGMEDGLVKVVSPIEDTPADRAGVKSGDLIFKIDDTLVKGLTLTEAVKRMRGKPK
                     TQIKLSILRKGENKPVEITLTREIIKVQSVKSKLVEDGYGYLRITSFQENTAAALIKH
                     LNELYKPGPLKGLVLDLRNDPGGLLNTAIGVSSAFLPPKTLVTSTDGRTPDAKHKFYS
                     SPEDYLRRSGQDDYIKNLPAEARTVPMVVLINGGSASASEIVAGALQDNKRAIIMGTT
                     SFGKGSVQTVLPLPGNTAIKLTTARYFTPSGRSIQAKGIAPDIVVEEKANGSSSMRLR
                     EADLEHHLENNKDGKDKDAATPAKPQSKAPAKSKGNPSRDEDDEASEPPSRELASKND
                     YQLGQAINLLKGLQIMQNRP"
     gene            complement(8635..9864)
                     /locus_tag="PLD53_03235"
     CDS             complement(8635..9864)
                     /locus_tag="PLD53_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC59950.1"
                     /translation="MKRLACALLFALLCSGLPMPDDALAKEESSAPPTRADVAEKKED
                     LQELRGQIKSLRKGVVAAEGKRSDVADQLKDVEQKISATLHDLRNLTTLRVKQQAVLD
                     DLASQARELENHLDGQQVQLGKLVYRQYLLGRPDSLRLLLNGDDPNQMARDLHYLAII
                     GRARSQLLNEIESSLQRKQALATETRKRTEEMVTIEARQKEQHGKLVAQREQRQVVLD
                     SISKDIAQQKRKIGDLQRDEKQLSSLISRLATKLVAKPTPTKPPAKGISNESTPEATP
                     AGNFAQLKGELRLPVRGVVSNRFGGPRQEGSTWKGLFIRSGQGSDVKAIAAGRVVFAD
                     WMRGFGNLMIIDHGSSYLSIYGNNDALLKQVGDNIQGGATIAAVGNSGGNPESGLYFE
                     LRHDGQPLDPLKWVNLK"
     gene            10170..10478
                     /locus_tag="PLD53_03240"
     CDS             10170..10478
                     /locus_tag="PLD53_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRC59951.1"
                     /translation="MAIQVSIIDKQEHIEQAARALKSISHPLRLKILCVVGDQEACVQ
                     EIVDAVGTSQSNISQHLAILREKGVLLTRKDANRVYYRVGDARTLQLIGMMREVFCGD
                     "
     gene            10596..11015
                     /locus_tag="PLD53_03245"
     CDS             10596..11015
                     /locus_tag="PLD53_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRC59952.1"
                     /translation="MEFIQQNIYLISIALISGGMLAFMSFRRPAGSNTLTSTQATLLI
                     NRENAQVIDLREPDEYISGHLPESRNIPAGRLQERAGELDRFKEGPLLLICQSGSRSS
                     TASKQLAKLGFARVHCLEGGINAWRTAGLPLKKGAKK"
     gene            11036..11287
                     /gene="grxC"
                     /locus_tag="PLD53_03250"
     CDS             11036..11287
                     /gene="grxC"
                     /locus_tag="PLD53_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HRC59953.1"
                     /translation="MYSTAVCPFCARAEQLLYARGVSEIEKVRIDLDPARRAEMMEKT
                     ARRTVPQIYIGDTYVGGCDELFALDHAAKLLPLLSGEAV"
     gene            11344..11832
                     /gene="secB"
                     /locus_tag="PLD53_03255"
     CDS             11344..11832
                     /gene="secB"
                     /locus_tag="PLD53_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HRC59954.1"
                     /translation="MTEQAAPSQEAPSFAIEKLYVKDLSLEVPNAPEIYLERDAPEVN
                     IQLQTGVRTMGDGVYEVTLTVTVTAKLAEKTVFLVEAGQAGIFRVKNVPEENLQPLLS
                     IACPNILFPYSRELISESITRAGFSPVILQPVNFEALFAARAQQEQAAAAAGTAGVET
                     IQ"
     gene            11888..12337
                     /locus_tag="PLD53_03260"
     CDS             11888..12337
                     /locus_tag="PLD53_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRC59955.1"
                     /translation="MKRLPNVLLLFFFVASGFSMSANAIEFRTVNAATVLYDAPSQRG
                     SKLFVIRSDTPVEVVVRLEGWSKVRDAEGGLAWIEQTYLSDKHSVIVTADRADVRRKA
                     DDSSSLVFEAEKNVALEFVEMAPGGWIKVRHRDGASGYVRANQIWGY"
BASE COUNT         2195 a         3571 c         3771 g         2837 t
ORIGIN      
        1 gcttcctgtg gcttaagctg gtaaccaata ccgagacggt gaacaagcgc tcgggttgat
       61 cgaactgcgg tgtcggtttc agcgtttctc cgcatcatcg cgctgatgcc gctgcttctc
      121 ccgtccgttt tgtccgggtc gctggctctc cggttgatgt tgagtgttgg ttgctgcgcg
      181 cgcctccgca gaccggctgg cctggccgcc ggcattgcgc ggtacttcat ggcggggttc
      241 atggactacc ggcattggcg tttcagggcg tacgggcggc gcttgtcgca cctgttgctg
      301 gctcacttcg ctgcgggctt cacgctgggg cgagcgcatc gcgggttctt cgcgccggtt
      361 ggggcgttcc tgggcgcgcg actcaaacgg ctcggttctg gaccagggcg ctgtccgtgt
      421 attttccggg actgcccgtg cgttttcctg tggcgcaaca cgtggcgcgg cctcaatgcg
      481 cgattcgcgc tgcggttggc gcattgcagt ctcttcgcgc cggtcgggac gttccggggt
      541 acgcggctcg aacgatccgg gtctggattg agtctctgtc cgtgcatttt ccgggactgt
      601 ccgtacgttc tcctgtggcg caacacgtgc agcggcctcg atgcggggat cgtgctgcgg
      661 ttggcgcatc gcaggttctt cgcgtcgggc agcgcgttcc tgcgtgcgcg gctcggacga
      721 tccaagtctg gactctggtg ttggccgcac gttgtctggc gatccccgtt cgctcctcaa
      781 cggcacggcc tcagaggagg gtcgcgctga cgagcgtgcc cgtggctggg aatcgcgcat
      841 ttccggagcg gtcgggtttg caacaaccgg cgtttggcgc tctcgttcct gtcgggcaac
      901 ggatgggccg gaacggtcgc ggggctcgtg tctgctgcgt gattcgatct gcggtggggg
      961 aggcgtggga attggggctg cggcctgcag cggctggttg cgaagtgcgt gatgatctct
     1021 gggcgatagc gcagccgact gtaccgggcg acggtgagtg acgacatcgg caggcacaaa
     1081 cgtcactgcc cgtggttgct cgcggtgagc gtaacgcgtc ttctgtacga catgctgagg
     1141 tcggttgacg atctctgtca cgttgttaat gtgggtgacg tgcgtgacat tgatgtggcg
     1201 tacgtatttc gggctgctgc gataggcggg tacgtagacc tcgcgcgggg cgagcggaaa
     1261 ccagccgacc gtgggtctgg tgccgatggc gattgaaatg ccgattcccg gtgatccgat
     1321 ccaggcgacc atggctggcg agtaggccgg ccgggcaacc cgtacacccg gcacccagcc
     1381 ccaggctccg cggtggtgta cccagcggcc gtagtggaag ggagcaaagc cccagggttc
     1441 ataggcgacc caactccatc cccacggcgc gacccaggcc cagtgcccgt tccggtaggg
     1501 cgcccagtca ggtgcgacag cgcgcgggta ccagatggcc ccatactcgg tgttttgcga
     1561 ccagctgcca taggcgtcga ggtcctcggc gccggtcatt tcgggcgaaa catagcgggt
     1621 gtaggcggtg gacgccggtc gccggtcacg tgcggcgctc cactgggtga aatcgtcatt
     1681 gatggccgtc gacagacggt agcgcgtctg gtcaccgccc tgtccggttt gccagaactg
     1741 cgcgctttgc ccggcaccga tttcgaggct gctgtctttc gactcgaaat gcagcgagcc
     1801 gtagtacgcg gtggcgctgg aactgttggc atccgcgtca aagcggtaga tccctgcatc
     1861 gtttgcgttg aaccgaccgt ttggcgtgtt cagttcgaat tcgcgtgcga catcgcgcga
     1921 cggcagtttg gcgattgccc ggcccccgct caggtagagg cgtacctgtt catcgtcgat
     1981 ccgcgagaaa tccagcgtcg tgtccgaatc gaggcgaacg gtcatcgagc cgatctggat
     2041 ttcggcctgg gaaccgggcc cggtggtcag gacgtcgcca ctggtgagcg ggtggttcag
     2101 cggtgcgctg tacgattcgc cggtctccgg gttgtacagg ctgaccgcgc ccgagccgga
     2161 gccggatagc caggcgatgc gtccgatgcg tcccggcgga tcagccaggg ctggcgtggt
     2221 gaacaagagg gcacaggcca gcgcggcgac cagcttgtac cagtggtgcg gggagagtag
     2281 cgagttgagc atggtgttcc gtccagagtc gggttggtct gggcccgcgt gcagccttgg
     2341 cctttgaaca acctaacgtc cgaagcggcg cttcggctac aacggataca ctttgttgca
     2401 caatcgaaca actgtttcaa atactgaagt accgagtacg ccctgagaaa ccgggttact
     2461 cctcgagtat cacgttggtg taaacgtcct gcacatcgtc gaggctttcc agtacatcga
     2521 tcagcttctg cattctgacg gcatcgtcgc cagtcaattc ggtttcattt tcggccttca
     2581 tcgtgacctg cccgaactcg gcgctgaagc ctgcggcctc aagggcttcc ttgacgctca
     2641 tgtattcact gggcggggta atgacttcga tcgagccgtc gtcgttgctc agcacatcgt
     2701 cggcaccggc ttcgatggcg gcatccatca gtgcggcctc atcggttccg ggcgcaaaga
     2761 gcagttggcc gcagtgcgta aactggaatg cgacacagcc gtcggtcccc atgttgccgc
     2821 cgtgcttgtt gaaggcgtgg cgaacttccg ccacggtacg cgtgcggtta tcggtcaggc
     2881 aatcgacgat caccgctgca ccgctgatgc cgtagccttc gtagcgaatt tcgacgtaat
     2941 cgaccccttc gagttcgccg gtgcctttct tgatggcgtt atcgatcttg tccttgggca
     3001 tgttgacgcc cttgccctta tcgaccgcca ggcgcaagcg cgggttgaag cctgggtcac
     3061 caccccccat tttggccgca acggtgattt ccttggcgat cttggagaac atcgcgcccc
     3121 gtttttcatc ctgccgtcct ttacggtgct ggatgttcgc ccatttggaa tgtccggcca
     3181 tgctaacctc ttctcttgca gcgtttgcaa ccggcctgtt ctaaaaacac ggacggttgc
     3241 ggatagttga tggttgatac ttgataaaat cggcagatca ccaagccgcg cattttaaca
     3301 ccacccgtcc gccccttatc tacggagtgc ctcatggccg aaccgctcat tgttgcccga
     3361 cacaaactga ccgatctggg actgcttccc gcactggcca accgccatgg attgattacc
     3421 ggtgcaaccg gtaccggcaa gacgatcacc ctgcaggtac tggccgagcg gttttcggcg
     3481 attggcgttc cggtgttcat ggctgacgtc aaaggcgacc tttccggtct ttcggcgccg
     3541 ggggtcgaga gtgcgaagtt caagcagcgg cttgacacgc tgggtatcag cgactggact
     3601 ggggcaaaat ttccggcgtc cttctgggat gtcagcggtg agcaggggca tccggtccgc
     3661 gcaacgattt cggacatggg accgattctg ctttcgcgcc tgctcaatct caacgacacc
     3721 cagtccggcg tgctgcaact ggtgttcaag atcgccgacg acaacggcct cctgttgctc
     3781 gacctcaagg atctgcgcgc catggttcag catgtcggcg aaaacgcgaa aacgtttacc
     3841 accgagtacg gcaatgtttc agccgcgtcc atcggagcga tccagcgcgg cttgctgacg
     3901 ctgggcgagc agggtgggga agccttcttt ggcgagccga tgctcgatat cgccgatctc
     3961 atgcagaccg atggcggcaa gggcattgtg aacattctcg cggcagacaa gttgatgtcg
     4021 tcgccgcgtc tctattcgac ctttctgctc tggctgctct ccgagctgtt tgaaaatttg
     4081 cccgaggtgg gcgatgcgga caagccgaag ctggtctttt tcttcgacga agcgcacctg
     4141 ctgttcaatg aagcgccacc cgcgctgctg gagaaaatcg agctggtcgt gcggctgatc
     4201 cgctcaaaag gcgttggcgt ctacttcgtg acgcaaaacc cgctggatat ccccgacagc
     4261 gtgcttggcc agctcggcaa ccgggtgcag cacgcattgc gcgccttcac gccacgcgat
     4321 cagaaagcgg tcaagacggc ggcagacacc ctgcgcgcca atccggcctt cgatgcggcg
     4381 gcggcgatta ccgaactggg tgtcggcgag gcgctggtct cgtttctcga tgaaaagggg
     4441 cgaccgaatg tcgtcgagcg cgccttcatc ctgccgccgg catcgcggat cggtccattg
     4501 accgcggagg agcggcaggg cgtcatcaag gcttcgctgg tctacggtgt ttacgaaaca
     4561 gcgctggatc gtgagtcggc ctacgagaag ctgaagagca agcggaccgc cgaggcagcg
     4621 ccgacgcagc cgtcggcgcc agcgcaggtt ccggcgaatg cgcccgcatc cggcggtggc
     4681 tggtttggcg gattggggga ccttattggc ggcagttcgg gaagtggtgg ccgtagcagg
     4741 ggcggtgatt cgatggtcga aatggccgcc aaaagtgccg cgcgcgccat cggttcgcaa
     4801 gtcggacgct caatcatccg tggggtgctc ggatcgatcc ttggcggttc gcgccgacga
     4861 tagttccgaa ggcgaacagt tttcagtcgc ccgcagggcg ggaacacgat ggcagcgtca
     4921 ttgcatgact tatgaatgca ggatccgctg atgaccgtgt gctcttatcc atccaactca
     4981 ggaatccggt ttgaaacgtc tatctgccga acgccttggc gtggtcctcg ccatcttcgc
     5041 ggcgctcggt ttttcgttca aggcgattct gatcaaattg gcctatgcgg cagcgccggt
     5101 cgatgcggtg acgctgcttt ccattcgtat gatttttgcg ctgccggctt tcctatgggt
     5161 gggtttcgtg gtcagtcgct cggcaccgcc attgacacgg cgcgactggg gcaaactcgt
     5221 ggcgctcggc ttgctcggct actacggggc cagcattctc gacttcatcg gactgcagta
     5281 catttcggca ggacttgagc gcctgatcct gtttacctat ccgacactga ccgtgatgat
     5341 cggtgtgctg ttcatgggca aggcgctgga aatgcgcgag gtgatttcac tgctgctctc
     5401 ctattgcggc atcggcctgg ctttcgcgca tgacctccag gtggccaacg acatggcgac
     5461 ggtcctgatc ggcggagcat tcgtgtttgc gtcctcactc tcgtacgcct tctacctgtc
     5521 gggtagcggg ccaatgatcc agcgcctcgg ttctgcacgc tttacggcgc tggccatgct
     5581 ggtgtcgaca ctggcggcgc agttgcattt ctttgccgta caaccgatct ccgcattcat
     5641 tcaaccgctt ccggtctatg cctactgttt ggcgatggct ttgttttcca cggtgctgcc
     5701 ggtgttcatg caatcggcgg cgatccggcg catcgggtct tccaaggccg tgctgatcgg
     5761 cacgctgggg ccggtgctga ccatattctt cgggtggtgg ctgctcggcg agccgctttc
     5821 gctggcacag atgggcggtg ccgggctggt ccttaccggc gtctggctgg tttcgcggcg
     5881 ctgagaagta ggggaagaga ctggatggac cacgcctcac tccagtgcgc gttcaccctg
     5941 ttccgcaaac agcgcacttc gaatccttgc cgaactttac gcagcgccaa tccatcgtca
     6001 aaccatcaaa gagcagcagt cgtcccacgg ccggctcgcc tgttccgcag atgactttca
     6061 aggcttcggc cgcctgcatg gcgccgatga tgccggtgag cggcgcgaag acgcccatca
     6121 ccgcgcagcg ctcttcctgg acgtcttcgc tttccggaaa caggcagtgg taacagggcg
     6181 agtcttcgcg ccgcgagtcg aacacggaga gctgcccgct gaaacggatt gccgcgcctg
     6241 aaatcagcgg cgtgcgatgt ctgacgcagg cgcgattgac cgcgtaccgc gtggcaaaat
     6301 tgtcgcagca gtcgagtacg acatcggcat cataaaccaa agcttccagc cgttcgttgg
     6361 caaggcgctc gcaaatcact tcgaccgtca cttccggatt gatttgcaac agcgccgttc
     6421 gccccgaaac cgctttgaac tgaccgacac gctcctgcgt atgcagaatc tggcgctgca
     6481 ggttggtcag gtcaacccgg tccccgtcgg ccagtgtgat ccgcccgacg ccggcggagg
     6541 cgaggtaaag cgcagcaggt gagccgaggc cgccagctcc gatcaccagt gcgcgtgcgc
     6601 caacaatttt ggcctggccc tcgatgccga tttcgtcgag caggatatgc cggctgtagc
     6661 gcagcaactg ctcgtcattc atctggtctg aacggtgtct gttgaacgag gtcgcgcgct
     6721 ctttcgaggc tggcctgcct cgtatcgcct ggcactattt atactcacgc cattccggcg
     6781 gggtatcgtc gtggtcgccg tgcgggtggc gttcccaggc atgcgtgtag gcttcgtgct
     6841 gcgatttctt gagcgggcgc tcgggcgcat cgagattatg caattgggtt tcttccacgt
     6901 agtagatcag cgcgcgcatc atcttgctca tcagggtatt gtcgaggtga taacccttat
     6961 cctcgagggc gccttcgagc tcctgcaggg agtgctgatg aagatcatag gccacaccga
     7021 cagcccggcg ctcgacacgt ggctcgacct ccacgtgttc aatcttgctg agaaagtcgg
     7081 aggcctccgg cacctgaccg ggggggaact tcgaaaacag aacattccgt tccttgcgga
     7141 tcggtggcgt cttgtttggc gaagctgccg gtcgtttcat ctttcacgtc cggatgacgg
     7201 ctacggcctg ttctgcatga tctgcagtcc cttgagcagg ttgatcgcct gacccagctg
     7261 gtaatcattt ttcgatgcca gttcgcggct gggtggttca gacgcttcgt cgtcctcatc
     7321 cctggacgga ttgcccttcg atttggctgg cgccttgctt tgcggtttgg ccggcgtggc
     7381 agcgtccttg tctttgccat ccttgttgtt ttcaagatga tgttccagat cggcttcgcg
     7441 caggcgcatc gaagagctgc cgttcgcctt ttcctcgacc acgatgtcgg gcgcgatgcc
     7501 tttggcctga atggaacgac ccgacggcgt gaagtaacgc gctgtcgtca gcttgatggc
     7561 cgtgttgccg ggcagcggca gcacggtctg caccgagccc ttgccaaagc tcgtggtacc
     7621 catgatgatc gcgcgcttgt tatcctgcag cgcaccggca acgatttcgg aagctgaagc
     7681 cgaacccccg ttgatcagaa cgaccatggg caccgtgcgt gcttcggccg gcaggttctt
     7741 tatgtaatcg tcctgcccac tcctgcgcag gtaatcctcc ggcgacgagt agaacttgtg
     7801 cttggcatcc ggtgtgcggc catcggtcga ggtcaccagc gttttcggtg gaaggaacgc
     7861 ggacgatacg ccaatggccg tgttcagcag ccctcctgga tcgttgcgca gatcaagcac
     7921 caaacccttg agcggccctg gtttatagag ctcgttgagg tgtttgatca gtgcggcagc
     7981 cgtgttttcc tgaaaggacg tgatgcgcag atagccgtaa ccatcctcga ccagcttgga
     8041 cttgacgctc tgcaccttga tgatttcgcg cgtcaatgtg atctcgacgg gcttgttttc
     8101 gcccttgcgc aagatggaaa gcttgatctg ggtcttcggt ttgccacgca tgcgtttgac
     8161 cgcttcggtg agcgtcaggc ctttgaccag cgtgtcgtcg atcttgaaaa tcaggtcgcc
     8221 ggatttaact ccggcacgat cagcgggagt gtcctcaatc ggcgagacga ccttgaccag
     8281 gccgtcttcc atccccactt ctatgccgag tccgccgaac tcgccctgcg ttcccacctg
     8341 caaatccttg taggcgtcag catccagata cgtggaatgc ggatccagat tcgagagcat
     8401 tccgctgatg gcataagtaa tcagtttctt gtcgtcgacc ggctcgacgt aaccctgctt
     8461 gatggcgttg aaaaccccgg caaaagtgcg caactcctcg atggggaggc tggtgggggt
     8521 ctctttttcg gccatcgccg aaaactgcag gctgatcagg atgccgccca gtattccggc
     8581 gcaaatcagc ccggcttgct tcaacttacc catcaatttc gctccaagac attatcattt
     8641 caggttgacc catttaaggg ggtcgagcgg ttgtccgtca tgccgcaatt caaagtataa
     8701 accggattca gggttgccac cgctgttccc cacggcggcg atggtggcgc ccccctgtat
     8761 gttgtcgccg acctgtttga gcagcgcatc gttgtttccg tagatcgaca gataactgct
     8821 gccgtgatcg atgatcatca gattgccgaa accgcgcatc cagtcagcaa atacgacgcg
     8881 gccggcggct atggccttga cgtcgctgcc ctgaccggag cggatgaaca gtcccttcca
     8941 ggtgctgccc tcctggcgtg gcccgccaaa acgattgctg acaacccctc ggacgggcaa
     9001 gcgcagctcg cctttcagct gcgcaaagtt cccggcaggc gtagcttccg gcgtgctttc
     9061 gttgctgatt cccttggccg gcggttttgt tggtgtcggc ttcgcaacga gcttcgtcgc
     9121 caagcgactg atcagactcg aaagctgttt ttcgtcgcgc tgcaggtcac cgattttgcg
     9181 cttctgctgc gcgatgtcct tcgagatgct gtcaagaacc acttggcgtt gctcgcgctg
     9241 ggcaacaagt ttgccgtgtt gttccttttg cctggcttcg atggtcacca tttcctcagt
     9301 gcgtttgcgg gtttcggtgg cgagtgcctg cttgcgctgc aaactgcttt cgatttcatt
     9361 gaggagctgg ctgcgtgctc gaccgatgat cgcaagataa tgaagatcgc gggccatctg
     9421 gttcggatcg tcaccgttga gcagaagccg gagcgaatcc gggcggccca gcaggtattg
     9481 ccgataaacc agtttcccca attgcacctg ctgaccgtcc aggtgattct ccaattcccg
     9541 tgcctggcta gccagatcat ccagaactgc ctgttgtttg acacgcagag tggtcaggtt
     9601 gcgcaggtcg tgcagagtcg cagagatttt ctgctcgacg tcttttagct ggtcggcaac
     9661 atccgatcgc ttgccctcgg cggctaccac gcctttgcgc aatgatttga tctgtccccg
     9721 cagctcctgc aaatcttcct tcttttcggc aacatcggct ctcgttggtg gagcactgct
     9781 ttcttccttg gccagcgcgt cgtcgggcat tggcaggccg ctacacagca gagcgaataa
     9841 caaggcgcac gcgagacgct tcaaggctgc tccggattcg gcgtgtgcgg gaacttgatg
     9901 tattgcgcgg ttaatggggt tcatacgact tggtggtctg cttcgcatat aacggaacat
     9961 aaaaacttgt ctttgcgcgt ctggcgttgt tgcgcatcct cgcgatagct ggctatcgct
    10021 gtggttcgcg cctcgccagt cgcgaaaatc cggcgttttc atcggtcccg ccatttgcaa
    10081 agcagaccac tcggggattg ccctgggggc gcccccagga tcgataatta ttctataatg
    10141 aatcccggta cttaacgagt gtctggtaag tggcaattca agttagcatc atcgacaagc
    10201 aggagcacat tgaacaggcg gcgcgtgcgc tcaagtcgat atcacacccg ttgcgtctca
    10261 agatactgtg tgtggtcggt gaccaggagg cttgcgtaca ggagattgtc gatgcggtcg
    10321 gcacgtctca gagcaacatc tctcagcatc tggccatatt gcgcgaaaag ggagttttgc
    10381 tgacgcgcaa ggatgccaac cgcgtttact atcgtgtcgg cgatgcgcgc accttgcaac
    10441 tgattggcat gatgcgcgag gttttctgcg gcgattaggt cactcgggat aagcaggcgg
    10501 cgagggttgc ccttttttcg ttttcgcgcc ttgattggcg tgtcccttga ttcccgcatt
    10561 tttttgtttt tttcttgcag tttctggagc ttcgcttgga attcattcag caaaacattt
    10621 atcttatctc cattgccctc atcagcggcg gcatgttggc attcatgtcc ttccgacgcc
    10681 cggccggcag caacacgctg acgtcaacac aggcgacgct actgatcaat cgtgaaaacg
    10741 cacaggtgat cgatttgcgc gagcccgatg agtacatttc cgggcacttg ccggaatcaa
    10801 ggaatattcc ggccgggagg ctacaagaac gtgccggtga actggacagg ttcaaggaag
    10861 gtccgctgct cttgatctgc caatccgggt cacgttcaag cactgccagc aagcaactgg
    10921 caaaactcgg ttttgcaagg gtccactgcc ttgagggtgg aatcaatgcc tggcgcacag
    10981 ccgggctgcc gctcaagaaa ggcgcgaaaa agtgagcgtt gcgccgcgtg tggtgatgta
    11041 ctcgacggcg gtttgtccct tctgcgcgcg ggccgagcag ttgttgtacg cacgaggtgt
    11101 ctccgaaatc gaaaaagtgc gtatcgacct tgatccggca cggcgcgccg agatgatgga
    11161 aaaaacagcg cgtcgcactg ttccacaaat ctacatcggc gatacctatg tcggtgggtg
    11221 tgatgagctg ttcgcgctcg accatgccgc caagcttctt ccattgttat caggcgaagc
    11281 ggtctgagac atggactatt tgccagatac ttccttcaac aattcaccac taaagaaacc
    11341 atcatgactg aacaagcagc cccttcacaa gaagccccct cattcgctat cgaaaaactc
    11401 tacgtcaaag atctgtcgct tgaggtcccg aacgctccgg aaatctatct ggaacgtgac
    11461 gctccggaag ttaacattca gctacagacc ggtgttcgca caatgggcga cggagtctat
    11521 gaagtgacgc tgacggtaac cgtgacggcc aaactggccg aaaagaccgt cttcctcgtc
    11581 gaagccggcc aggcgggtat cttccgcgta aagaacgtgc cggaagagaa ccttcagccg
    11641 ctgctgtcga ttgcctgtcc gaacattctg ttcccgtatt cccgtgagct catttccgag
    11701 tcgataacgc gtgcaggttt ctccccggtc attctgcagc cggtcaattt cgaagcgctt
    11761 ttcgctgctc gtgcccaaca ggaacaggcc gcggccgcgg cgggtactgc gggtgtcgaa
    11821 acgattcagt aaccaggtat caacaaattg ttgtccggat cttgaaagac aaagaggatt
    11881 gctcgaaatg aagcgactgc cgaacgtgtt gctgctcttt ttcttcgttg cctcaggctt
    11941 ttccatgtcg gcgaacgcga ttgaattccg aacggtgaat gccgcgacgg tgctctacga
    12001 tgcgccttcg cagcgaggca gcaaactgtt cgtaatcagg agtgatacac cggtagaggt
    12061 ggtcgtcaga ctcgaaggct ggtccaaggt acgggatgcc gaaggcggcc tggcctggat
    12121 cgagcagacg tacttgtccg acaagcactc ggtcatcgtc acggctgatc gagccgatgt
    12181 gcgtcgcaaa gcggatgatt cttcgtcgct ggtcttcgag gccgaaaaga acgttgcact
    12241 ggagtttgtc gaaatggcgc ccggtggctg gatcaaggtt cgccatcggg atggcgcttc
    12301 gggctatgtg agagccaatc aaatctgggg ctattaggtt tgaatctcac cgtcattgcc
    12361 gcgggtgcct gggg
//
LOCUS       DAORPM010000039        12052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1124_length_12052_cov_9.881637, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000039 DAORPM010000000
VERSION     DAORPM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1124_length_12052_cov_9.881637"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLD53_03265"
     CDS             <1..1164
                     /locus_tag="PLD53_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59956.1"
                     /translation="DTPATMEDVESEFGAPVAGLVEALTDVSRPSDGNRAVRKEIDRR
                     HTAHASPRAKTIKLADLIDNCEDIAHHDPRFAQVYLQEMEALLAVLGEGEPELMRQAR
                     ALHDSTQEKLRALQRTVPPPASASLAALIPHLDNPQIVRLFRETFSASDIVEPLLSFD
                     DDKAQSAVARVMQSRHLNIAGIRVDGTVQGYLRLTDMAAGDSAPSGQLMQHISADQVL
                     DGSAPLTDVFGILTRHEHCFVSMFDTIVGVITRNAVNKPVVRMWLFGAITLYEMGLVQ
                     FIERCFPGNTWEALLAPARLTKAWELQKERERRNLHSNLIDCLQFSDKMQILLEDQAA
                     LEALGIKSKRTAKLLTKELESLRNHLAHSQDIATHDWVQIIRITQRLSELGPQ"
     gene            1173..1967
                     /locus_tag="PLD53_03270"
     CDS             1173..1967
                     /locus_tag="PLD53_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRC59957.1"
                     /translation="MHTRTAAVLLATLLMLACAPTPRPTTSERSSNPPLAGTHWVLQA
                     LGSSAVAKTPPVTLNFGSDNRIGGNDGCNQYQGNHTSGDDSIRIGENLAGTLMACPDQ
                     LEAQARDYRQALQRAARFALDRDRLSLRDDAGNLLATFVPAVTSPVGTTWEVVLYNNG
                     KQGAVSLLLGSRISASFATDGRVSGSAGCNQYFAAYTLNGPSMRIGATGATRRFCAEP
                     DGMMNQERLYLEALQTTATFRLNGDRLELRTANGALAVSFRRETGE"
     gene            2168..2968
                     /locus_tag="PLD53_03275"
     CDS             2168..2968
                     /locus_tag="PLD53_03275"
                     /inference="COORDINATES: protein motif:HMM:NF022665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HRC59958.1"
                     /translation="MQSKVWAVVAIVLFGAAIAIYILPNREAEPLPPLLETTPPLTAP
                     IAQPETAPAEPAIAHPLETIAPVEPLPDLEQSDPSIFKPLIQVLGEQWKALLKPEALI
                     HKIVVTVDNLPRKDLPAKVVPLKRVKGAFLTSGKGDALSISVQNPQRYSSYVRLIQSV
                     DTEKLVDLYRQFYPLFQRAYIEIGYPKGYFNDRLVEAIDDLLASPEPDEPIKLVQPKI
                     LFKYADPELEARSAGQKIMIRMSRDNAGQLRTKLREIRQHVAKVPRTP"
     gene            3036..3497
                     /locus_tag="PLD53_03280"
     CDS             3036..3497
                     /locus_tag="PLD53_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HRC59959.1"
                     /translation="MNTKPALLVALALLANPSLAENVGCVTTEWKLIGANHKVCVESF
                     HDPKIPGVTCHVSQARTGGISGSFGLAQDPSQFSLACRQTGPITLPDKLPKDATVFTE
                     DTSIFFKETRVIRLWDEANRTLVYLAISRKLIEGAPANSISTVPVMPWGAR"
     gene            3655..4932
                     /locus_tag="PLD53_03285"
     CDS             3655..4932
                     /locus_tag="PLD53_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRC59960.1"
                     /translation="MNNKISSKCSLVAMPYLVILALLGACSTEATMPADPDQSAQKAA
                     PPAPPPPSPILQIPILPHDEAVLKAANTLFSKAELAGDPADKTMRRPVVIDPLIDGVS
                     RMQTAATRKMETNVANLVKAKYPQFEVKPFTAANLGQSPLLVVGTFTPINQLGKTEGV
                     RESYRFCLALVDLKSRRIVSKAVARSRMENVDSAPLPFFSDAPVWTRDPTGEAYVATC
                     QASKLDGEVSPAYIDSLVADALINEGIRAYNSAHFKEALALFVKAGATPAGQQFRTLT
                     GNYLAASKLGRRQESAQAFGRIVDFGLDAGKLSVVFTFRPGATSLHGGTAKEPYDMWL
                     KSLAQHAAQRNSCMEIIGHASRGGPESVNERLSALRAEYVRQRLVDETPSLDKRLIAA
                     GYGSRENLIGTGKDNASDALDRRVEFRLLACRE"
     gene            4949..7855
                     /locus_tag="PLD53_03290"
     CDS             4949..7855
                     /locus_tag="PLD53_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRC59961.1"
                     /translation="MIVSISRSVVALLRSGQSVSRFWLRRVVTAILLSAHLLAGAAEN
                     APLLRIETGTHTASIRSLAVDADGQIMLTASDDKTARLWRLSDGQPIGVLRPPIGPGN
                     EGKLYAAALTPQGDLAAVGGWSADNDVYIFRRESGAMRLRLSGMPNVINHLTFSPDGK
                     MLAVALWGGHGIRVFTSRNAWSSAALLGEDSAYGGDSYSVSFSSDGRRLTSTAYDGYV
                     RVYEITASGLSLIAKTRVSGGQHPFAARFSPDGKLLAVGFEDAGRVAVVKADDLTPAY
                     SPDIRGLSDGNLTSVAWSPDGNTLYAAGTSRKRASEHVLRRWDDGGRGAFTDVTVAGD
                     SVTGLAALPDQRVVFAAADASWGIVDAKGAVSYRQTVPLADFRANWSGFGIQQARHAI
                     GFAYGFGGRHPALFDLDNQVLILQDAALPKQAVGGKGLEVSEWKDSQKPLINGQAVAL
                     EPGEMSVSTSVSAAGDAAALGSNWFVRLFDANGRELWRTATPAPCWAVDIDNSDPAKP
                     WVVAALGDGTIRWYRRQDGAEQLALFPHADRQRWVMWTPDGRFSASQDGDGLVGWHVN
                     RGPDQSAEFLPVARFSERYYNPPAIAAVLSPPKASPASPPPKMADLRKGINLPPSVTL
                     LTPEAEKLAKNGRLRLEIVANDRGSGVDEIRLYHNGKIVADTAAIKTTRTAGKGHVIK
                     VYDVPLEAGKNVFRALALGSDRTESEAIERAIEYAASDQRARLYVLVVGINSYRNAVL
                     NLSFSVPDAQGMARFFRNNYGRLFKDVKVDEIYNNDATQTNIRSRLKELREARPDDVV
                     VVYLAGHGETLNEDWMFIPYDLVTPERPEVLQRDGISSRELAEAVKMIPAQKLMVIID
                     ACKSGAATISFRGLEERRALAQLSRSTGTHMIAATTKDQPASELDALGHGVFTYTLLE
                     GLMGKAASGEKDVTARKLMVFVEQAMPELTRRYRSEEQYPVVSSNGMDFPLALH"
     gene            8083..10704
                     /locus_tag="PLD53_03295"
     CDS             8083..10704
                     /locus_tag="PLD53_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRC59962.1"
                     /translation="MKRTLLAAIAAFVTLVAGIGLVQYQNLGAAHAENAPAAAPGNPG
                     TPGRPLPEAAAVDQDAPYFEACAREGLNESECVGRLIWFKATAGNDRFHTYTFQQRVG
                     VLVDWFRVLRSDQRDDRFWAWGIMNDPSCCKPGDPDCPAKTADETFGFDWCPGDDVLL
                     KYVGKPGYVDPACGLKDAALDADDPHSQGGKLDQRHSACDLKFGTSTGALGIRKFPNP
                     RFDAEKWKKLNGSMATWAGFNAAKSEQTGIESDQRVSKLADGSVEPPFLIGTACGSCH
                     IAFDPLNPPADPAHPKWENLKGLIGNQYTRMSELLVSGMPRSSLEFQMFAHARPGVTD
                     TSAISNDQINNPGTINALINVAQRPVFQGETITKWRKTNTCGTEKDESKCWCEPGRNN
                     KCWLQSTRNDDTTPVNLGGQKVVLPGVHHILKGSEDSTGALEAVQRVYFNIGSCSEQC
                     WINHFSDMRQVDPQQRGFNQTSFDIGQCRRDCPNFRAIEDRLQTVLDFFASAESDESD
                     LYAARANLRKAGNPSENYGRADLVAELEAEFGSGAVGRGQTVFAENCARCHSSIPENT
                     GGAFKNRDFAAPNEAHPRKVRADFLGNDQSTPVTEVGTFRCRSLHSNHEAGRLYMGYQ
                     SDSLRQRPIVADIPERNKLKDGGRGYMRNISLVNVWATAPFMHNNAIGPEICGKPANK
                     ENNFDRVRYVDADGKLLAEQPECLRFDPSVEGRFKLYKLSMHDLLNPKERGIKRTLTN
                     ADLLIDVGIRPLDGKTEKPLLGFGQVKIPAGSSVGFLNGLEHKQLVADLFLAKRDPAK
                     LEASGKKDLVPTLQAIADDVLKNPARFAEILREKRDFLKANYLTCTEEIENEGHRFGE
                     DLSESDKKALTAFLATL"
     gene            10724..11632
                     /locus_tag="PLD53_03300"
     CDS             10724..11632
                     /locus_tag="PLD53_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59963.1"
                     /translation="MRLPTIFRLLTGRITLAALAVPALFLTGCSKPEPPNIAFAPYDQ
                     TYSSKLDLAQVDYKYPIAPAELAKITPDYLATLDQEQLDQIYARLTAGPIPDGAFDGR
                     ILLPRGSSGKFRLSEIVGGFAGTALHLKGLVVENIGEALWRGKVFFRDERVLRNRIDD
                     LSVLKKIGLVEGEPKKMSYGGKETWLLFPAKLYCGQSLLDSRRESVIIDYFFTDEIPG
                     YQENPDFLAGRRGLRVRDEIRMIRPGFYLGRAYLDRGFALNFTLYDKATDEKAQEDFV
                     KTGKVQEDCWPGTQVRTAAVVAAAKP"
     gene            11728..>12052
                     /locus_tag="PLD53_03305"
     CDS             11728..>12052
                     /locus_tag="PLD53_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59964.1"
                     /translation="MSAPQADPPAWVVDPAMPGENLPPSGRSLFDRLFAAREKGVEGH
                     ETAGYDIPFPFTKLLTRLDTQLKRDPNSLLYPAKRVLIPLGRSLQRTAAAPDYFLFPR
                     VVVAVD"
BASE COUNT         2533 a         3732 c         3508 g         2279 t
ORIGIN      
        1 gacacgccag ccaccatgga ggacgtcgag agcgaatttg gcgcaccggt tgcggggctg
       61 gtcgaggcgc tgaccgacgt aagccggccc agcgacggaa accgtgccgt gcgcaaggaa
      121 atcgaccgcc gccacaccgc ccacgcctcg cctcgcgcca agacgatcaa gctcgcggac
      181 ctgatcgaca actgcgagga cattgcgcac cacgacccgc gcttcgccca ggtctatctg
      241 caggaaatgg aagcgctgct ggcggtactc ggtgaaggcg agccggaatt gatgaggcag
      301 gcgcgcgcgc tacatgacag cacgcaggaa aagctgaggg ccttgcaaag gaccgtaccg
      361 ccaccggcat ctgcgagtct ggcggccctg atcccacacc tggacaaccc gcagatcgtg
      421 cgcttgtttc gggagacctt cagcgcaagc gatatcgtcg aacccctgct ctctttcgac
      481 gacgacaagg cgcaaagcgc tgtcgcacgc gtcatgcaat ctcggcatct gaacatcgcc
      541 ggcatccgcg tcgatggcac agtccaaggc tacctgcgcc tgaccgacat ggcggccggc
      601 gactctgcgc ccagtgggca gctaatgcag cacatctcgg ccgatcaggt gctggatggc
      661 agcgcgccgc tcaccgatgt gtttggcatc ctgacacggc acgaacactg tttcgtctcc
      721 atgttcgata cgatcgtcgg ggttatcact cgtaacgccg ttaacaagcc ggtcgtccgc
      781 atgtggctgt tcggcgccat caccctctac gaaatggggc tggtccaatt catcgagcgg
      841 tgcttccccg gcaacacctg ggaagcttta ctggcccccg cccgcctgac gaaagcgtgg
      901 gagttgcaga aggaacggga gcgccgcaac ttgcatagca acttgatcga ctgcctgcaa
      961 ttttccgaca agatgcagat cttgcttgag gaccaggccg cgctggaggc cctgggcata
     1021 aaatcgaagc ggaccgccaa actactcacc aaggagctgg aatcgctgcg caatcacctc
     1081 gcacactccc aagacatcgc tacccacgac tgggtgcaga tcattcgcat cacccagcgt
     1141 ctgtcggaac tcgggccaca gtgagcgcct ttatgcacac aagaacggcg gcagtcctgt
     1201 tggctacact gttgatgctg gcttgcgcac ccactccacg cccgaccacg agcgagcgca
     1261 gcagcaatcc acccctcgca ggcacgcact gggtcttgca agccctcgga agcagtgcag
     1321 tcgcgaaaac ccctcctgtg acgctcaatt tcggctcgga caatcgtatc ggcgggaacg
     1381 acgggtgtaa ccagtaccaa ggaaaccaca cctcaggaga cgactcgatc cggattggcg
     1441 aaaatctggc tggcaccttg atggcctgcc cggatcagct tgaggcacaa gctcgtgact
     1501 atcgccaagc cttgcagcga gcagcgcgtt ttgcgctcga ccgggaccgg ctcagcctgc
     1561 gagacgatgc cggcaacctg ctcgccacgt ttgtaccggc ggtcacatct ccggtcggca
     1621 cgacatggga agtcgtgctc tacaacaacg gcaaacaggg cgccgttagc ctgcttctgg
     1681 gcagccgcat cagcgccagt tttgctactg acggtcgggt gagcggcagt gcaggctgca
     1741 accagtattt cgctgcctat acgctgaatg gcccaagcat gcgcatcggc gcgactggcg
     1801 ctacgcgcag gttctgtgcc gaacccgatg ggatgatgaa tcaggaacga ctttacctcg
     1861 aagcactaca gacgaccgcc acgttccggc tgaacggtga ccggctcgag ttgcgcacgg
     1921 ctaacggcgc gctggcagta tccttcaggc gcgagaccgg agagtagaaa gcagtcgggt
     1981 ccgggaaggc atcgcattcg acgagcgaat cttcagcgtg cctgccaagc ccgcagcctc
     2041 cggaaatcag cgcgaaagca cgacctgctt ccaggggacg ctgaatttct cccgctcgaa
     2101 aacgcacaag caaacggttg tggtctatcc tttgaacacc gaaaaaactg ccacgggaga
     2161 ctgattgatg caaagcaaag tctgggcggt tgttgccatc gtcctgtttg gtgcagcaat
     2221 tgcgatctac attctgccga atcgggaagc cgagccgctc cctcctttgc tggaaacgac
     2281 tccgccgctg actgcaccga tcgcgcagcc agaaacagcg cccgctgaac ccgcaatcgc
     2341 ccatccattg gaaacgatag cgccggttga accgttgccc gacctggagc agagcgatcc
     2401 gtcaatattc aagcccctga tccaggttct gggcgaacaa tggaaagcac tgctcaagcc
     2461 cgaagccttg attcacaaga ttgtcgtcac cgtggacaac ctcccacgta aggatctgcc
     2521 ggcgaaagtg gtgccgttga aacgcgtgaa aggtgctttc ctgaccagtg ggaaaggcga
     2581 tgcgctttcc atcagtgtgc agaacccgca gcgctactct tcctatgtcc ggctgatcca
     2641 gtccgtcgat accgagaaac tggtcgattt gtatcgccag ttctacccgc tgtttcagcg
     2701 cgcttatatc gaaatcggct atcccaaggg gtatttcaac gaccgtctgg ttgaggccat
     2761 cgacgatctt ctcgcgtcgc cggaaccaga cgaaccgatc aagctggtac agccgaaaat
     2821 tctcttcaag tacgccgatc cggagctgga agcacgttcc gccggccaga aaatcatgat
     2881 ccgcatgagc cgcgacaacg ccggacaact caggacgaag ttgcgcgaaa ttcggcagca
     2941 cgtcgcgaag gtgccacgca ccccatgaac gatctacctt cgaacatgga ccgttcaccc
     3001 gcaccgaacc tcccgggaag ttggaatttg atcctatgaa tacgaaacct gccctgctcg
     3061 tagcactcgc gctattggcc aacccctcgc tcgcggagaa tgtcggctgc gtgaccaccg
     3121 aatggaagct gatcggcgcc aatcacaagg tatgtgtcga gtcgtttcac gaccccaaga
     3181 taccgggcgt gacctgccat gtcagccagg cgcgaaccgg cggcatcagc ggttcgttcg
     3241 ggctggcgca ggatccttcg caattttctc tggcttgccg acagacgggc ccgatcacgc
     3301 tgcccgacaa gctgcccaag gacgccacgg tattcaccga ggacacctcg atcttcttca
     3361 aggaaacacg cgtcatccgc ttgtgggacg aagccaaccg cacgctggtg tatctggcga
     3421 tcagccgcaa gctgatcgaa ggcgcgccgg ccaacagcat ttccacggtg ccggtcatgc
     3481 cttggggcgc ccgctaagct tcagcctcaa tgggcggtct gtttcgtaat acgctgcttg
     3541 cccatttgac aaacgactga actcggctgg gtttgcgcgc tctaatgctt gccaactaca
     3601 gtgctacctg ctgccgaagg tcttttcgtc accggcgtga tacgggagag acagatgaat
     3661 aacaagattt cgtcgaagtg cagtttggtt gctatgccgt acctggtgat cctggcgttg
     3721 ctgggcgcct gctccactga agctacaatg cccgctgacc ccgatcagtc ggcgcaaaaa
     3781 gccgcacccc cggcaccccc accgccttcc ccgatcctgc aaatcccaat tctgccgcac
     3841 gatgaagccg tcctcaaggc cgccaatact ctgttttcca aggcggagtt ggccggcgat
     3901 ccagcggaca agacaatgcg tcggccagtc gtcatcgacc cgttgatcga cggcgtgtcg
     3961 cgcatgcaaa ctgccgcgac acggaaaatg gaaaccaatg ttgccaatct ggtgaaggcg
     4021 aaatatcccc agttcgaggt caagccgttt acggcagcca atcttgggca atcgcccttg
     4081 ctggtcgtcg gcaccttcac gccgatcaat cagctgggta aaacggaagg cgtacgcgaa
     4141 tcgtatcgat tctgcctggc tctcgtcgac ctcaagtcaa ggcgtatcgt cagcaaggcc
     4201 gtagcgcgtt cgcgtatgga aaatgtcgat tcagcaccct tgccattctt cagtgatgct
     4261 cctgtctgga cgcgggatcc gaccggcgag gcttatgtcg caacgtgcca ggcttccaag
     4321 ctggacggcg aagtcagccc tgcctatatc gattcactgg ttgccgacgc actgatcaac
     4381 gaaggcatcc gcgcctataa cagcgcacat ttcaaggaag cactggcgct ctttgtcaaa
     4441 gccggggcca cgcctgccgg acagcaattt cgcacgctga caggaaacta tctggcggcc
     4501 agtaagcttg ggcgccgcca ggagagcgct caggcattcg gcagaattgt cgacttcgga
     4561 ctcgatgcgg gcaagctctc tgttgtgttt accttccgtc ccggcgcaac ctcgctacac
     4621 ggcggcaccg ccaaggaacc ctacgatatg tggctgaaga gccttgcgca gcatgcggcg
     4681 cagcgcaatt cgtgcatgga aatcattggc cacgccagtc gtggcgggcc ggaatccgtc
     4741 aatgagcgcc tgtccgcctt gcgcgccgaa tacgtccgtc agcgactggt cgatgagaca
     4801 ccatccctgg ataagcgtct gatcgctgcc ggttacgggt cgcgtgaaaa cctgatcggg
     4861 actggaaagg acaatgccag cgatgcactc gatcggcggg tcgaattccg cctgctcgct
     4921 tgccgcgaat gactcggaaa tggtcgtgat gattgtatcc atatctcggt cggttgtagc
     4981 cttgttgcgt agtgggcaat ccgtgagccg cttctggcta cgccgcgtcg tcactgcgat
     5041 cctgttgagc gcccacctgc ttgctggggc ggctgaaaac gcgccgttgt tgcgcatcga
     5101 aactggtacg cacacggcgt cgattcgcag ccttgcggtg gacgccgacg gacagatcat
     5161 gctcacggcg tccgacgaca agaccgcgcg gctgtggcgg ctttccgacg gccagccgat
     5221 cggcgtcctg cggcccccga tcggtccggg gaatgaaggc aagctctacg ctgcggcgct
     5281 aacgccgcaa ggcgatctgg ccgcagtcgg cggctggagt gccgacaacg acgtctatat
     5341 tttccggagg gaaagcggcg ccatgcgcct gcgcctgagc gggatgccca atgtgatcaa
     5401 ccatctgacc ttttcgccgg acggaaaaat gctggcagtc gcactttggg gcggccacgg
     5461 catccgggtt ttcaccagcc gcaatgcctg gtcgagtgcg gcgctgctgg gtgaagacag
     5521 cgcctatggc ggtgattcgt acagcgtcag ctttagttcc gacggccgcc gactgacgag
     5581 taccgcgtat gacgggtacg tccgcgtgta cgaaattacg gcgagcggac taagcttgat
     5641 cgccaaaaca cgcgtcagcg gcggccagca cccctttgcc gcacgctttt cacccgacgg
     5701 gaaacttctg gcggttggat tcgaggatgc ggggcgcgtc gcggtagtga aagccgatga
     5761 tctgacgccg gcctattcgc ccgacatccg cggcctctcc gacgggaatc tgaccagcgt
     5821 cgcctggtcg ccggatggca atacactcta tgctgccggc acttcgcgaa aaagggcgtc
     5881 cgagcacgtt ttgcgacgct gggacgatgg cggccgcggc gcgttcacgg acgtcacggt
     5941 cgccggcgat agcgtcaccg gtctcgctgc gcttcccgat cagcgcgtgg tatttgccgc
     6001 tgcggacgca agctggggaa tcgtcgatgc caagggtgca gtcagctatc gtcagaccgt
     6061 gccgctggct gattttcggg ccaactggtc tggcttcggc atacagcaag cgcgtcatgc
     6121 catcggtttt gcgtacggct ttggcgggcg gcatccggcg ctgtttgacc tcgacaatca
     6181 ggtgctgatt ctccaggacg cagcgcttcc caagcaggca gttggcggca agggcctgga
     6241 ggtcagcgag tggaaggatt cgcagaaacc cctgatcaat ggccaggcgg tcgccctgga
     6301 acctggggag atgagcgttt cgacatcggt ttcagcggcc ggcgatgcgg cagccctggg
     6361 cagcaactgg ttcgtcagac ttttcgatgc aaacgggcgc gaattgtggc gaaccgcgac
     6421 gccggcgccc tgctgggcgg tggatatcga caattccgac ccggcgaagc cctgggtagt
     6481 cgccgcactc ggcgacggaa ccatacgctg gtaccgacgc caggacggtg ctgagcaatt
     6541 ggcgctgttt ccgcatgcag accgtcaacg ctgggtgatg tggacgccgg acggacgctt
     6601 ttccgccagc caggacggtg atggcttggt cggctggcac gtcaatcgcg gtcctgatca
     6661 atccgccgaa ttcttgccgg tggcgcgctt cagcgagcgc tattacaacc cgcctgccat
     6721 cgccgctgtc ttgtcgccgc cgaaagcgtc tccagcttcc ccgccaccca aaatggctga
     6781 cctgcgcaaa ggtatcaatc tgccgccgag cgtgaccctg cttacgccgg aagcggaaaa
     6841 gctggccaag aatgggcgtc tgcgccttga aatcgttgcc aacgaccgcg gcagcggagt
     6901 cgacgagata cgcttgtatc ataatggcaa gatcgttgcc gacacggctg cgatcaaaac
     6961 gacccgcacc gcgggcaagg gacatgtcat caaggtttac gacgtgcccc ttgaagccgg
     7021 caagaatgtc ttccgcgcac ttgcactcgg ttccgaccga acggaatcgg aagcgatcga
     7081 acgcgcgatc gagtatgcag ccagtgatca acgggcacgc ttgtatgtcc tcgtggtagg
     7141 catcaattcc taccgcaatg ccgtcttgaa cctgagtttc tcggtacccg atgcgcaagg
     7201 catggcccga ttctttcgta ataactacgg tcgactgttc aaggatgtca aagttgacga
     7261 gatctataac aacgatgcca cgcagaccaa tattcgctca cgcctgaagg aattgcgcga
     7321 ggcccggccg gacgatgtgg tggttgtata tctcgccggt cacggcgaga cgttgaatga
     7381 agactggatg ttcattcctt acgatctggt gacacctgaa cgcccggaag tattgcaacg
     7441 tgacgggatt tcctcgcgcg aattggcaga agccgtaaaa atgatcccgg cgcaaaagct
     7501 gatggtcata atcgacgcgt gcaaatcggg tgctgcgaca attagcttcc gtgggctcga
     7561 agaacgacgc gctcttgccc aactgtcgcg ctcgaccggc acgcacatga tcgcggcgac
     7621 caccaaggat caaccggcct cggaactgga tgcgctggga cacggagtat tcacctatac
     7681 cctgctggaa ggcctcatgg gtaaggcagc aagcggcgaa aaagatgtaa ccgctcgcaa
     7741 gctgatggtc tttgtcgagc aagcaatgcc cgaactgacg cggcgctatc gttcggaaga
     7801 acagtacccg gtggtcagct cgaacggaat ggattttcca ttggcgttgc attgaacctg
     7861 ctagctctgc cctgttacac atatgtcatg cgcacgcaaa gaccgttgcc tccggcatcc
     7921 gaacaaggta aaaagcaaac ctcggcattg agccgtagcg ctagtttgaa aaaagcacag
     7981 aactaatacg gtctttgaac ttcaaaacac tacgattgtc actgacagat gatcggggcc
     8041 ccggcggacc gggggatcag aacgttgaac gggagacaac gaatgaaacg tacattgctg
     8101 gccgctatcg cggcattcgt gacccttgtc gccggtatcg gactcgtcca ataccagaat
     8161 cttggtgcag cacatgccga aaacgcgccg gcggcagcac caggcaatcc agggactcct
     8221 ggccgaccac ttccggaggc cgcagccgta gaccaggatg cgccctactt cgaggcctgc
     8281 gcccgcgagg gcctcaatga atcggaatgc gtcggacgcc tgatctggtt caaggcgact
     8341 gccggtaacg accgtttcca tacctatacc ttccagcagc gtgtcggcgt gctggtggac
     8401 tggttccggg tactgcgcag cgatcagcgc gatgaccgct tctgggcctg gggcatcatg
     8461 aacgacccgt catgctgcaa accgggcgat cccgactgcc ctgccaaaac tgcggatgaa
     8521 accttcggct tcgactggtg tcccggagac gacgtattgc tcaagtatgt cggcaagccc
     8581 ggctatgtcg acccggcttg cgggctcaag gatgccgcgc tggacgccga cgatccgcac
     8641 agccagggcg gcaagctgga tcagcgccat tcggcctgcg atctcaagtt cggcacatcg
     8701 acaggcgccc tgggcatccg caagttcccc aacccgcgct ttgatgccga aaagtggaag
     8761 aagctgaacg gcagtatggc cacctgggcc ggcttcaacg ccgccaaatc ggagcaaacc
     8821 ggcatcgaat ccgatcagcg ggtgagcaag ctggccgatg gctcggtcga gccgcctttc
     8881 ctgatcggca ccgcctgcgg ctcgtgtcac attgctttcg accccctgaa tccgccggcc
     8941 gatccggcgc atccgaaatg ggagaacctc aaggggctga tcggcaatca atacacccgc
     9001 atgtccgagc tactggtttc cggcatgccc aggagcagtc tggagtttca gatgtttgcg
     9061 cacgccaggc caggagtcac cgacacctcg gccatttcca acgaccagat caacaacccg
     9121 ggcaccatca atgccctgat caatgtcgcc cagcgcccag tgttccaagg tgaaaccatc
     9181 accaaatggc gcaaaaccaa cacctgcggc accgagaagg acgaaagcaa atgctggtgc
     9241 gaacccggcc gcaacaacaa atgctggttg cagagcacgc gcaacgacga tacgacgcca
     9301 gtcaatctcg gtggacagaa ggtcgtgctg cccggtgtcc atcacatact caagggtagc
     9361 gaggattcca ccggcgccct ggaagccgtc cagcgggtct atttcaacat tggttcgtgt
     9421 tccgagcaat gctggataaa tcacttctcg gacatgcgcc aggtcgaccc acagcaacgc
     9481 ggattcaacc agacatcgtt cgacattggc caatgccggc gcgattgccc gaacttccgc
     9541 gccatcgagg atcgcctcca gaccgtcctg gatttcttcg cctcggccga gtccgatgaa
     9601 agtgacctct acgccgcccg tgccaatctg cgcaaggccg gcaatccttc ggagaactac
     9661 ggtcgcgcgg atctggttgc cgaactggaa gccgaattcg gtagcggagc cgtcggtcgc
     9721 ggccagaccg tctttgccga aaactgcgca cgctgtcatt cgagcattcc ggaaaacacc
     9781 ggcggcgcat tcaagaatcg cgatttcgcc gcgcccaatg aagcacatcc gcgcaaggtg
     9841 cgcgccgatt tcctcggcaa cgaccagtcg acaccggtta ccgaggtcgg caccttccgt
     9901 tgtcgttcgc tgcactccaa ccacgaggcg ggtcgcctct acatgggcta tcaatccgac
     9961 agcttgcgcc agcgtccgat tgttgccgac atcccggagc gcaacaagct gaaagatggc
    10021 ggtcgcggct acatgcgcaa catctcgctg gtcaatgtct gggccaccgc gccattcatg
    10081 cacaacaacg ccatcggtcc ggaaatctgc ggcaaaccgg ccaacaagga aaacaacttt
    10141 gaccgcgtgc gttacgtcga tgccgacggc aagttgctcg cggagcagcc cgagtgcctg
    10201 cgttttgacc cgagcgtcga aggacgcttc aagctctaca agctttcgat gcacgacctg
    10261 ctaaacccga aggagagggg tatcaagcgc acgctgacca atgccgatct gctgatcgac
    10321 gtcggcatcc ggccgctcga cggcaagacc gagaagccgc tgcttggctt tggtcaggtc
    10381 aagatcccgg cgggcagcag tgtcggcttc ctcaacgggc tagagcacaa gcaactggtt
    10441 gccgaccttt tcctcgccaa gcgggatccg gccaagcttg aagcctcggg caagaaggac
    10501 ctcgtgccaa cgctgcaggc catcgccgac gatgtcttga agaacccggc acgctttgcg
    10561 gaaatcctgc gcgagaagcg cgatttcctc aaagcaaatt acctgacctg caccgaggaa
    10621 atcgagaatg aaggccaccg cttcggcgaa gacctttccg aatccgacaa gaaggcgctc
    10681 acagccttcc tcgccacgct ctaaggctcc gggagatcac cacatgagac tgccgaccat
    10741 tttccgtctg cttaccggcc gaatcacctt ggccgccctc gctgttccag cgctcttcct
    10801 caccggatgc agcaaaccgg aaccgccgaa cattgccttc gcaccctacg atcagacgta
    10861 ctcgtcgaag cttgatctgg ctcaggtcga ctacaagtac cctatcgccc cggccgaact
    10921 ggcaaagatc acgccggatt acctcgccac cctggatcag gaacagcttg accagatcta
    10981 tgcccgtctg acggccggcc ccatacccga cggcgcgttt gatggccgta ttctgctgcc
    11041 gcggggcagc agcggcaaat tccgcctttc cgaaatcgtc ggcggctttg ccggcacggc
    11101 gctgcacctc aagggtctgg tcgtcgagaa tatcggcgaa gcgctgtggc ggggcaaggt
    11161 gtttttccgt gacgagcggg tgctgcgcaa ccgcatcgac gatctatcgg tcctcaagaa
    11221 gatcggtctg gtcgaaggcg agccgaagaa gatgagttac ggcggcaagg aaacctggct
    11281 actcttcccg gccaagctgt actgcggcca gagcctgctc gattcgcgcc gcgagtcagt
    11341 gattatcgac tacttcttca ccgacgaaat cccggggtat caggagaacc cggattttct
    11401 ggcgggtcgt cgcggactgc gcgtgcgcga cgaaatccgc atgattcgtc ccggtttcta
    11461 ccttggaagg gcctaccttg atcgcggctt tgccctcaat ttcacgcttt acgacaaggc
    11521 aaccgatgaa aaggcgcagg aggatttcgt caagaccggc aaggtacagg aagactgctg
    11581 gccgggaacg caggtgagaa ccgccgcggt tgtcgctgcc gccaaaccct gagcggtgct
    11641 ggcacgtgaa tcgggcaatt cgccatgaag ttgtcttcgg ctcttctcgc cgtcctctgg
    11701 tgcctggccc cttgcagtgg aacggccatg tcggcccccc aggctgatcc tccggcatgg
    11761 gtagtcgatc cggcaatgcc gggcgagaac ctgccgcctt cgggccgttc gctgtttgat
    11821 cgcttgttcg ccgcccgcga gaagggggtt gagggtcatg aaacggcggg ttatgacatc
    11881 ccttttccct ttacaaagct gctcacccgc cttgataccc aactcaagcg cgatcccaac
    11941 agtctgctgt acccggccaa gcgggttctg attcccttgg gtcgttcgct gcagcgtacc
    12001 gcggcagcgc ccgattactt cctgtttccg cgcgtggtgg ttgccgtgga tt
//
LOCUS       DAORPM010000040        11957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1142_length_11957_cov_9.717106, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000040 DAORPM010000000
VERSION     DAORPM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_11957_cov_9.717106"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLD53_03310"
     CDS             complement(<1..495)
                     /locus_tag="PLD53_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRC59965.1"
                     /translation="MELKKVLLIGGSGFVGGWIANRLSERGIRVTIPTRRRDNTKQLI
                     LLPTVYMEEADVNDPQVLASLMDGVDAVINLVGILHDSDSSQPYGKRFAAAHVELPRK
                     IIAAMQQTGVRRLVHMSALGAATDAPSAYLRSKAEGEAAVCAAMNATGAHEGSIDVTV
                     FRPSV"
     gene            complement(534..2477)
                     /locus_tag="PLD53_03315"
     CDS             complement(534..2477)
                     /locus_tag="PLD53_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRC59966.1"
                     /translation="MKFSLVAFVYVLSLLLAVPAAASVSADDRFLAARDAVKAGDRAK
                     LERIAPELKGHALEAYVEYWQIQLDLENPDPGTIRAFLTRYDKSYLAEKLRADWLKQL
                     GKRNQWKAFDDEYPFLVKPDQELACYALQSRRARGDASMLDDAMPLWLNLIEPPASCY
                     PVLEALILEKRVLADGVWARIRRQYEANKLSAARYTMNYLPPSQTPDARTATAVSDTP
                     LPWLLKLPPSFSDSQMNRELAALAISHIARNDPRMAAEQLSRIERQLQAGEIAWAWSQ
                     IGWQAAKRHMNEAVQWYRKGDDAPLSDEVAEWKVRAALRAQDWGTVRSTIEKMSPELA
                     EQPAWVYWLGRAYRAGGRVEDANTLYARIAGQPNFYGNLADDELGRPIMTPPKAAPPT
                     PAEMAQVEANPGVQRALALYRVNLRSEGFREWNWTLSSMSDRELLTASEIAERAGIFD
                     RAIAAADRTKNEHNYALRYLSPYSDQVRPAATNQSLDSAWVYGLMRQESRFITNAKSS
                     AGASGLMQLMPATAKWVARKIGLKDFRQGQVNDTETNLLLGTTYMRLVMESLDNHPVL
                     ASAAYNAGPGRARKWRADHQLEGAIYAETIPFNETRDYVKKVMSNSVYYSALFEGRPQ
                     TIKSRLGVIGPRATVDPKAEVLP"
     gene            2536..3147
                     /locus_tag="PLD53_03320"
     CDS             2536..3147
                     /locus_tag="PLD53_03320"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRC59967.1"
                     /translation="MPETKLYISADARAVCRGELRKQGIERRLALSTDVCAVLSAKVC
                     EHLKDHFPQLARMRVGFCWPVKNEPDLRPLVMSWIASGQAGFAALLPVVLGIGSALAF
                     REWVPGTPLVADRYGIPTPREGAFQVPEALLLPVNAFDAAGYRIGYGGGFFDRTLAAL
                     GPTPLSIGVGFELARVDSIHPEPHDVRLDAMVSEAGVFRHAPS"
     gene            complement(3154..4239)
                     /locus_tag="PLD53_03325"
     CDS             complement(3154..4239)
                     /locus_tag="PLD53_03325"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRC59968.1"
                     /translation="MRASDSRLAFIDALKAVASQLIVLHHLAFYGPMSDVANGLAPAL
                     FSWLSQDARIAVQIFLVIGGFLAARTLAPTGRLIVANPLTVLSDRYFKLVIPYVAAIF
                     LSILCAAIARSLMDHDTIPDPPTLRQMLAHVALLQNILAYDSLSAGVWYIAIDFQLFV
                     LLLGTLWFARAVGDRFISLPVFSILFVSVLALGSLFYFNRDSDWDNWAIYFFAAYALG
                     TLTYWVTESERNPDWLPVIFCVVVVALIIDFRSRIAVALITALALGISRRWGFMESRP
                     GGKFLAYLGKISYSVFLVHFPVLLVMNALFSRIAPENPTANAFGIGLAWAASVAAGAV
                     FYRFVESQARRWQGPTLPRKHQSLHVQ"
     gene            complement(4421..8041)
                     /locus_tag="PLD53_03330"
     CDS             complement(4421..8041)
                     /locus_tag="PLD53_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRC59969.1"
                     /translation="MSDRDSLEQHLLEYKALIDNAGVAIVLTRERLVRRCNPYAHELF
                     GWPPGKLIGQAGSVFLPSAEAYVTLLQKVRPELRAGKTVEVESPLKRHDGSIFSAHLV
                     VRAIDAAAPRLGTVWIINDISQECTRRDATARLLREQQLIFESAHTGIVFLRERIIVR
                     CNPRFEEILGYGPGELIGKLNSIYYPSEEDWIETGRIAYAAIAETGTYSGEARYQRRD
                     GTPIWCHITGSMLDPQNPDLGFVWLYEDITARHNAEAALIQSYREHQLIFDNALIGIS
                     YQRNRVIMRCNRRLEEIFGFPEGGLAGQSTRVLFCSEEEWKDAWVQVNSQLSAERTFE
                     GEMRYCKRDGTPIWNHIIGRTITSPDGGETWIWAYDDITARRAAEAALVQSNQEYSLI
                     FNNAMIGISYMRNRTFLHCNRRLEEIFGYPPGGLIGQSSRLLFDTDEEWEETGRRVYD
                     TTKAGDFFSGEISYRRSDGQRICVNARGRMVEQAGEQIWIWTHEDVTRRFLAEEALRK
                     SNIELEQRVTERTAELSKQLHFLKQLIETIPGPIFYKDEHARYLGCNSAFANFIGIAA
                     GDLIGKTPHDIAPAELADKYLAADRTLLDKPGTQIYECPVRYANGEIRDVMFHKATFT
                     QPDGAVGGLVGFMLDITERKRMEASLRQAATVFDSSAEGVTITSPDGTIVAVNRAFTE
                     ITGYSEAEVLGRNPSMLQSGRHNKAFYRDMWTAVERSGRWQGEVWNKCKNGRLFPEWL
                     TISAVKDSSGAITHYVGVFSDITAIKTAYEQLNYQAHHDSLTGLPNRLLLEDRLHVAL
                     QRARREQTGLAVLFVDLDRFKKINDSLGHDVGDRVLCEVAVRMSKLIRGSDTVARLGG
                     DEFLILMEGVGDSGTALNVADKILENLRETPVSLDQEFFVGVSIGISIFPEDGDDATT
                     LIKNADIAMYRAKQRGRNTSEFYTPELTDSALEHFHLETELRRAIERDELRIYLQPQF
                     SLVTGKLIGAEALVRWQHPEQGLVLPGKFIPLAEESGLIVDIGEWVQRAACQYWAAWT
                     REGLNPGVVSINVSSVEFRRGRIQEIVRRTLDATRLPPALLELEITESAVMSQVESGI
                     QILNDLRAIGINLAIDDFGTGYSSLAYLKRLPLNKLKVDQSFVRGLPDNAEDCAIARA
                     VIALGHSLQLTIIAEGVETHEQSEFLLREGCDEIQGYLRGKPMPVDEFARAFLLDGLT
                     PA"
     gene            complement(8177..8773)
                     /gene="slmA"
                     /locus_tag="PLD53_03335"
     CDS             complement(8177..8773)
                     /gene="slmA"
                     /locus_tag="PLD53_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HRC59970.1"
                     /translation="MAKPGERKLQILQTLAGMLESPKAEKITTAALAARLECSEAALY
                     RHFASKAQMFEGLIEFIESSLFGVINQISAEETQGLQQVEHILALLLNFAQRNRGMTK
                     VLTGDALVNENERLQLRINQLLDKIEAALKQALRVAATQEQISAGADFGALANVLLCY
                     IIGRWQQYAKSGFTREPMAQWPQQWPMLLVACLSQRPN"
     gene            complement(8812..9456)
                     /locus_tag="PLD53_03340"
     CDS             complement(8812..9456)
                     /locus_tag="PLD53_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HRC59971.1"
                     /translation="MADGSRTWLFDLDNTLHDVNPSILPHIDRSMTAYIREHLCVDEG
                     EAARLRRHYWLRYGATLLGLMRHHGTDPEHFLHHTHQFPDLASSVIVERGLKAMLRRL
                     KGRKIIFSNAPFKYTSAVLAIIGIRHCFDAIYTVERLRFQPKPAVSGFLHLLHKEELD
                     PRSCIMVEDTQLNLKTAKRLGMRTVWVSTCTRHPPYVDVKLASVLDLPQRLGRL"
     gene            complement(9456..10343)
                     /gene="argB"
                     /locus_tag="PLD53_03345"
     CDS             complement(9456..10343)
                     /gene="argB"
                     /locus_tag="PLD53_03345"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRC59972.1"
                     /translation="MTSQHLSPAQEAAILAEALPYIKRFHGKAIVVKFGGNAMVNEKL
                     KQGFARDVVLLKLVGMHVVVVHGGGPQIEDLLTRVGKKGEFIQGMRVTDAETMGIVEM
                     VLGGQVNKDVVNLINQAGGKAVGLTGKDSNLIRAKKLLLPNRESPADLIDIGQVGDIT
                     QIDPSLIAHLESGGFIPVIASIGVGKDGETYNINADVVAGKIAEVLKAEKLVLLTNTP
                     GVLDREGKLVTDVTPKQIDEMVSDGTLSGGMLPKINSALDAARNGVKSVHVIDGRVEH
                     ALLLEILTSHGVGTMIKSH"
     gene            10468..11028
                     /locus_tag="PLD53_03350"
     CDS             10468..11028
                     /locus_tag="PLD53_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRC59973.1"
                     /translation="MSDPDLQQALQKLRPTLLRFAMLQLRDEAAAEDVVQETLLAALE
                     GKSSFSGRSQLKTWAVSILRNKIVDLMRRGSREGKLPLVDEHGDEDFDALFDGSGHWQ
                     EPPGDWGDPAAVLEQRDFYDVLELCMKALPENVARVFTMREVLGFETEEICKELKMST
                     SNCWVVLYRARMRLRECLQLRWFSVR"
     gene            11034..11207
                     /locus_tag="PLD53_03355"
     CDS             11034..11207
                     /locus_tag="PLD53_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRC59974.1"
                     /translation="MKSCREIHRLVIEGQDRELGFAERFSMRVHLMICTTCKRFDAQI
                     DLMRQALRRFPGD"
     gene            11360..11905
                     /locus_tag="PLD53_03360"
     CDS             11360..11905
                     /locus_tag="PLD53_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HRC59975.1"
                     /translation="MLIPRQEVPALKVETLSTGSFDLASEGPANFTLLVFYRGLHCPI
                     CAKYLLELARLLPDYVQRGVGVIAVSSDTRERALEMQKKLGVAEQQSLRFGYGLPLEK
                     AREWGLYLSASRGKTSIGIEEPALFAEPGLFLVRPDRTLYYGAVQTMPFARPHFDELL
                     QAIDFAIDKNYPARGEYTGPG"
BASE COUNT         2419 a         3403 c         3556 g         2579 t
ORIGIN      
        1 gaccgagggg cgaaacacgg tgacatcgat actgccttcg tgcgcacccg tggcattcat
       61 ggccgcgcat accgccgctt cgccttccgc tttcgagcgc aaataggccg atggcgcgtc
      121 ggtagcggcg ccgagcgcgc tcatatgcac cagacgacgc acgccggtct gttgcatggc
      181 cgcgatgatt ttcctgggca attcgacgtg cgcggcagca aaacgtttgc cgtaaggctg
      241 gctggaatcg ctatcgtgca gaatgccgac cagattgatg accgcatcga cgccgtccat
      301 caatgaagcc agcacctgtg ggtcgttcac atcggcctct tccatgtaga ccgttggcaa
      361 caatatcaac tgcttcgtat tgtcgcgacg acgcgtcgga atcgtcaccc gtatgccacg
      421 ttcggaaagc cggttggcta tccagccgcc aacgaaaccg ctacccccaa tcaacaatac
      481 cttcttgagt tccataacac cctgacgctg tagtaagtaa taaagagccg attttacggt
      541 aaaacttcgg cctttggatc aacagtcgcg cgtggtccaa tcacgccaag ccggctcttg
      601 atcgtctgcg gtctgccctc aaaaagtgcc gaataataga ccgagttgct catcaccttt
      661 ttgacgtaat cccgcgtttc gttgaaagga atagtctcgg catagatcgc accctccagt
      721 tggtggtcgg cacgccattt gcgggctcgg ccggggccgg cattgtatgc agcagaagca
      781 agcaccgggt gattgtcgag gctttccatc accagacgca tgtacgtggt gccgagcagc
      841 aaattggtct cggtgtcatt cacctggcct tgacgaaagt ccttgaggcc gatttttctt
      901 gcgacccact ttgccgtcgc cggcatcaat tgcatcaggc cggatgctcc cgccgatgac
      961 ttggcattgg tgatgaagcg gctttcctga cgcatcaggc cgtacaccca ggcgctatcc
     1021 agagattgat tcgtcgccgc cgggcgcacc tgatcgctgt agggcgacag gtagcgtagc
     1081 gcgtaattgt gttcgttctt cgtacggtcg gcggcggcga tggcgcgatc gaaaattccg
     1141 gcgcgctctg cgatttccga agccgtcagc agctcccggt cgctcatgct actcagtgtc
     1201 cagttccact cccggaagcc ctcacttcgc agattgacac gatacagcgc gagggcgcgc
     1261 tgcacaccgg gattggcctc aacctgtgcc atctcggcag gcgtgggggg cgccgccttg
     1321 ggcggtgtca tgatcggccg ccccagctca tcgtccgcca gattgccgta gaagttcggt
     1381 tgcccggcga tccgggcgta aagcgtattg gcatcctcga cgcgcccccc tgcccgatag
     1441 gcgcgtccca gccagtaaac ccaggccggt tgttcggcaa gttcgggcga catcttttca
     1501 atcgtcgaac gcaccgttcc ccagtcctga gcccgcaggg ccgcgcgcac tttccactcc
     1561 gccacttcat cggaaagtgg cgcatcgtcg cctttgcgat accactgcac cgcttcgttc
     1621 atgtgtcgct ttgccgcctg ccagccgatc tgactccacg cccaggcaat ttcaccggcc
     1681 tgtagctggc gctcgatcct gctcaactgc tccgctgcca tacgcggatc attgcgtgcg
     1741 atgtgactga tcgccagcgc ggccagctcg cgattcatct ggctgtcgga aaacgacggt
     1801 ggcagtttca acagccacgg caatggggtg tcgcttaccg cagtcgctgt ccgggcatcg
     1861 ggagtctggc tgggtggcaa atagttcatg gtgtagcgcg cggcggacaa cttgtttgct
     1921 tcgtattggc gacggatgcg tgcccagacc ccatcagcca gtacgcgttt ctccagaatc
     1981 agcgcttcca gtaccggata gcacgatgca ggtggctcga tcagattcag ccagagcggc
     2041 atcgcatcat cgagcatgct cgcatcgccg cgtgcccgac gactctgcaa ggcataacag
     2101 gcaagctcct gatcaggctt gacaaggaac gggtattcgt cgtcaaacgc cttccattga
     2161 ttcctcttgc ccaattgctt caaccagtcc gctcgcagtt tctcggcaag atagcttttg
     2221 tcgtagcgcg tcaggaaagc tctgatcgtc cccggatcgg ggttctccag atcaagctgg
     2281 atctgccaat actcgacata ggcttcaagg gcgtggccct tcagttcggg agcgatacgc
     2341 tcaagcttgg cgcggtcgcc tgccttgacc gcatcgcgcg cagcgagaaa ccggtcatca
     2401 gcactcacgc tggcggccgc aggcactgcc agcaacaggg aaagcacgta gacgaaagca
     2461 acaagactga acttcatgat aagctggaat ccccgtaaac actgactttc agcataccac
     2521 acgagctgcc ggttaatgcc agaaaccaaa ctgtatatta gcgcagacgc gcgcgctgtc
     2581 tgccggggcg aattgcgcaa gcagggcatc gaacgccggc tggcgctgtc gaccgacgtt
     2641 tgcgcggttc tttcggcgaa ggtttgcgaa catctgaaag atcacttccc tcaattggca
     2701 agaatgcgcg tcggtttctg ctggccggta aagaatgaac ccgatctgcg accgctggtg
     2761 atgtcgtgga tcgcatccgg ccaggccggg ttcgcggcac tgctcccggt tgtcctcgga
     2821 atcgggtcgg cactggcttt tcgcgaatgg gttcccggca cccctctggt tgcagaccga
     2881 tacggtattc caacgccgcg tgaaggtgca tttcaagtgc ccgaagcctt gctgcttccg
     2941 gtcaatgcgt ttgatgccgc cggataccgg attggttatg gtggaggctt cttcgatcgc
     3001 acgctggctg cactcggccc aacgccgctg agcatcggcg ttggcttcga attggcccgt
     3061 gtcgactcga ttcacccgga gccacacgat gtccgcctcg acgccatggt cagcgaagcc
     3121 ggcgtgttca ggcatgctcc ttcctagggg tgtttactgc acgtgaaggc tttggtgctt
     3181 cctagggagg gttggcccct gccagcgacg cgcctggctt tcgacgaaac gatagaacac
     3241 tgcgcccgcg gcaacgctgg cagcccatgc cagcccgatg ccaaaggcgt tggcagtggg
     3301 gttctccggc gcgatcctgg agaaaagagc gttcatcacg agaagaaccg ggaagtggac
     3361 caggaacacc gaataggaaa tctttcccag gtaggccaga aatttgcctc cggggcgact
     3421 ttccatgaat ccccagcgtc gggagatgcc aagcgcaagc gcagtgatca gggcaaccgc
     3481 aatgcgggag cgaaagtcga tgatgagcgc aacaacgact acacaaaaaa tcaccggcag
     3541 ccagtccgga tttcgttcgg actcggtcac ccagtacgtc agcgtgccca gcgcgtaggc
     3601 agcaaagaaa tagatcgccc agttatccca gtccgaatcg cggttgaaat agaacaggga
     3661 gcccagcgcc agcacgctca cgaacaaaat gctgaaaaca ggcaggctaa tgaatcgatc
     3721 tcccacggcg cgggcaaacc agagcgttcc gagcagcagg acaaagagct ggaagtcgat
     3781 ggcgatgtac cagacgccgg cagacagcga gtcataggcg aggatattct gcaaaagggc
     3841 aacgtgcgcc aacatctgcc gcagtgttgg cggatcggga atcgtatcgt gatccatcag
     3901 actgcgtgcg atggccgcgc agagaatgct tagaaatatg gcggccacat agggaatgac
     3961 cagcttgaag tagcggtctg acagcaccgt gagcgggttg gcgacaatca gtctgccagt
     4021 gggtgccagg gttcttgctg ccaggaaccc gccaatcaca agaaatatct ggactgcaat
     4081 tcgcgcgtcc tgactcaacc acgaaaacag tgccggagcg aggccgttgg cgacatcgga
     4141 catcggaccg tagaacgcca gatggtggag aacgataagt tgtgaggcga cggctttgag
     4201 cgcgtcgata aaggccagtc gtgagtcaga ggcacgcatg cgaaatccgg gaaattactt
     4261 gagcggtgca gcctggacga tacggaaagg ctcgatttca gagcatagga tgctggagcg
     4321 aaaccaccat ggccagagac gtccgtcgct gtaaggggcg aatattacga cagacttgtt
     4381 ttgttaacaa tactaaattc tggcccactc gcaccggcct tcaggctggc gtcagtccgt
     4441 cgagcaggaa agcccgggca aactcgtcga caggcatcgg cttgccgcgc agataaccct
     4501 gaatctcgtc gcatccttca cgaagaagga attcactctg ctcgtgcgtc tcgacgccct
     4561 ccgcgatgat ggtcagttgc aaactgtggc cgagggcgat gacggcgcgc gctatggcgc
     4621 aatcttctgc gttgtccggc agtccgcgca caaaactctg gtcgaccttc aatttgttta
     4681 gcggcaaacg tttcaggtac gccagtgacg aatagccagt gccaaaatcg tcgatggcga
     4741 gattgattcc aatggcgcgc aggtcgttca agatctgaat gccactctcg acctgactca
     4801 tgacggcgct ttcggtgatc tccagttcca gcagggcagg tggcaggcga gttgcatcaa
     4861 gcgttctgcg cacgatctcc tgaatgcggc cacggcggaa ttcaacactg gaaacgttga
     4921 tcgaaaccac tcccggattg aggccttccc gcgtccaggc cgcccagtat tgacaggcgg
     4981 cacgctgcac ccattcgccg atgtccacga tcagcccgga ttcctcggcc aacgggatga
     5041 acttgcccgg cagaaccagc ccctgctcgg ggtgttgcca gcgaaccagc gcttccgcac
     5101 cgatgagttt gcccgtgacc agcgagaact gtggctgcag gtagatgcgc aattcgtcgc
     5161 gttcaatggc gcggcgtagt tcggtttcca gatgaaaatg ctcaagagcg ctatcggtca
     5221 gttcgggggt atagaactcg gaggtgttgc gaccacgttg tttggcgcga tacatcgcaa
     5281 tgtccgcgtt cttgatcagc gtcgtcgcgt cgtcaccgtc ttccgggaaa atgctgatgc
     5341 caatgctgac gccgacgaaa aactcctgat ccagcgacac cggcgtttca cgcaggtttt
     5401 caaggatctt gtcggcgaca ttcaatgctg ttcccgaatc gccaacgccc tccatgagaa
     5461 tcaggaactc gtcgcccccg aggcgggcga cggtatccga gccgcggatg agcttgctca
     5521 tccgcaccgc cacttcgcag agtacgcggt cgccgacatc gtggccgaga ctgtcgttga
     5581 tcttcttgaa gcgatcgagg tcgacgaaca gtacggcgag tcccgtttgc tcgcggcggg
     5641 cgcgttgcag tgcgacatgc agccgatctt caagcaacag gcggttcggc aatccggtga
     5701 gcgagtcgtg atgggcctgg tagttgagct gttcgtaggc tgtcttgatc gctgtgatat
     5761 cggagaaaac gccgacatag tgggtgatgg cgccggatga atccttgacc gcgctgatcg
     5821 tcagccattc cggaaacagt cgtccgttct tgcatttatt ccagacttca ccctgccatc
     5881 gaccgctgcg ttcgacggca gtccacatgt cgcggtaaaa cgccttattg tgacgaccgg
     5941 attgaagcat gctcgggttg cgcccgagga cctctgcctc gctgtagccg gtaatttcgg
     6001 tgaaagcacg gttgacggcg acgatcgttc cgtcgggcga agtaatggtg acgccctcgg
     6061 cgctgctgtc gaagaccgtg gccgcctgcc gcaggctggc ttccatgcgt ttgcgttcgg
     6121 taatgtcgag catgaagccg accagtccgc ccacggcgcc atcgggttga gtgaaggttg
     6181 ccttgtggaa catcacatcg cgtatctcgc cattggcata gcgtaccggg cattcgtaga
     6241 tctgcgtacc gggtttatca agcaatgttc gatcggccgc gaggtacttg tcggccagtt
     6301 cggccggtgc gatgtcgtgc ggcgttttgc cgatcaagtc gccggccgcg attccgataa
     6361 aattggcgaa agcgctgttg cagcccaggt agcgagcatg ctcatccttg taaaagatcg
     6421 ggccgggaat ggtttcgatc agttgcttca ggaaatgcaa ttgcttcgag agttccgccg
     6481 tacgttcggt tacacgctgc tccagctcga tgttgctctt gcgcaaggct tcctcggcaa
     6541 gaaaacgacg ggtaacatcc tcatgcgtcc agatccagat ttgttcgccg gcctgctcga
     6601 ccatccggcc cctggcattg acgcaaatgc gctgcccatc ggatcgccgg tagctgattt
     6661 cgccggaaaa gaagtcaccg gccttggttg tgtcatagac acggcggccg gtttcctccc
     6721 attcctcgtc ggtgtcgaaa agcagtcgtg acgactggcc gatcaggccg cccggcggat
     6781 aaccgaagat ctcctcaagg cgccggttgc agtgcagaaa cgtgcgatta cgcatatacg
     6841 agatgccgat catcgcgttg ttgaatatca gcgaatattc ctggttgctt tgcacgagtg
     6901 ccgcttcggc agcacgacgg gcggtgatgt cgtcataagc ccatatccag gtttcgccac
     6961 catcgggtga cgtgatggta cggccgatga tgtgattcca gatcggagtc ccgtcgcgct
     7021 tgcagtatcg catctcgcct tcgaaggtcc gttcggcaga caactgcgaa ttgacttgca
     7081 cccaggcgtc cttccattcc tcttcgctac aaaagaggac gcgcgtcgac tgcccagcca
     7141 ggccgccttc aggaaaaccg aatatctcct ccagccgacg gttgcagcgc atgatcacgc
     7201 gattgcgctg atacgaaata ccaatcaacg cgttgtcgaa aatcaactgg tgttcgcgat
     7261 aactctgaat cagggccgct tcggcattgt gacgggcagt gatgtcttca tatagccaga
     7321 caaaaccaag gtccgggttt tgcgggtcaa gcatgctgcc ggtgatgtgg caccagatcg
     7381 gcgtgccgtc acgcctttgg tagcgcgcct caccgctgta ggtgccggtt tcggcaatgg
     7441 cggcataggc gatccggccg gtttcaatcc agtcttcttc gctcggatag tagatgctgt
     7501 tcaattttcc gatcagctct ccgggcccgt agccgagaat ctcttcaaag cgcggattgc
     7561 agcgcacgat aatgcgctcg cggagaaaaa caattccggt atgggcgctc tcgaagatga
     7621 gctgctgttc gcgcagcagg cgagccgtgg cgtcgcgcct ggtgcattct tgggagatgt
     7681 cgttgatgat ccagacggta cccaagcgcg gtgccgcagc gtcgatggca cggacaacca
     7741 gatgggcact aaaaatgctg ccgtcgtgac gctttaaggg agattcgacc tcaactgttt
     7801 ttccagcacg caattccggc cgtactttct ggagtagggt tacgtaggcc tctgcacttg
     7861 ggaggaaaac cgatccggct tgtccgatca gctttcctgg cggccagccg aaaagttcat
     7921 gggcgtaagg gttgcagcga cgaaccagac gttcgcgcgt taaaacgata gccacccccg
     7981 cattgtcgat gagcgccttg tattccagga gatgctgttc aagcgaatcg cgatcactca
     8041 tcgttcaagt ggccttgtcc gagggggttt gaggcaaagg tatcaggctg gctggctgcc
     8101 gtccacatca ataccacagg cgagtaccta ggttcggcag tgaggcatcg gggtgggccg
     8161 catcatgggc cgcggttcag ttcggccttt ggctcagaca ggcgacaagg agcatcggcc
     8221 attgttgcgg ccattgggcc atcggctcgc gagtgaagcc gctttttgca tattgctgcc
     8281 agcggccgat gatgtaacag agcaggacgt tggccagcgc gccgaaatcg gcgccggcgg
     8341 agatttgttc ctgcgtggca gcgacacgca gcgcctgctt gagtgcggcc tcgatcttgt
     8401 cgagcaactg gttgatgcga agctgcaggc gttcgttttc attcaccagc gcatcgccgg
     8461 tcaggacctt ggtcatgccg cgattgcgtt gggcgaagtt gagcaacagc gccaggatat
     8521 gctcaacctg ttgcaggccc tgggtttctt cggcagatat ctggttgatg acgccgaaca
     8581 gactgctttc gatgaattcg atcagcccct cgaacatctg cgccttgctg gcgaagtggc
     8641 gatagagcgc ggcttcagaa cactcaagcc tggcggccag ggcggcggtg gttatctttt
     8701 ctgccttcgg gctttccagc attccggcca gcgtttgcag aatttgcagt tttctttcgc
     8761 caggcttggc catgatgtcg ccctcagggg ttgattgttt ggcacggctg attatagccg
     8821 cccaagccgt tgcggcaaat cgagaactga ggccagtttc acgtctacat agggggggtg
     8881 gcgtgtgcag gtactcaccc acacggttct cattccgaga cgcttggccg tcttgaggtt
     8941 caactgtgtg tcttcgacca tgatgcatga acgcgggtcg agctcttcct tgtgcaacaa
     9001 atgcaggaaa ccgctgacgg ccggcttggg ttgaaagcga agtcgctcca cggtgtagat
     9061 ggcgtcaaag cagtggcgaa tgccgataat cgccagcact gctgaagtgt atttgaacgg
     9121 agcgttggag aagatgatct tgcgcccctt gagacgacgc agcatggcct tcagtccacg
     9181 ctcgacaatg accgagctcg ccagatccgg aaactggtgc gtatggtgca gaaagtgctc
     9241 gggatcggtg ccatggtggc gcatcaggcc gagaagggtg gctccgtagc gcagccagta
     9301 atgtcgcctg agccgcgccg cctcgccttc atccacgcac agatgctcgc ggatgtaggc
     9361 cgtcatcgag cggtcaatgt gcgggagtat ggacgggttg acgtcgtgaa gcgtgttgtc
     9421 gaggtcaaaa agccaggtgc gtgatccgtc tgccatcaat gcgatttgat cattgtgccg
     9481 acgccgtgac tggtcaggat ttccagcagc agtgcatgct caacgcgacc gtcgatgacg
     9541 tgcacgctct tgacgccatt gcgcgcagca tccagcgccg aattgatttt gggcagcatg
     9601 ccgccggaca gcgttccgtc ggaaaccatt tcgtcgatct gtttgggcgt gacgtcggtg
     9661 accagtttgc cttctctgtc cagcacaccc ggcgtgttgg tgagcagtac gagcttctcc
     9721 gctttgagca cctcggcgat tttcccggca acgacatcgg cattgatgtt gtaggtttcg
     9781 ccgtccttgc cgacgccaat cgaggcgatg accgggatga atccgcctga ttcgaggtgg
     9841 gcaatcagcg acgggtcgat ctgcgtgata tcgccaacct ggccgatgtc gatcaggtcc
     9901 gccgggcttt cccgattcgg cagaaggagt ttctttgcgc ggatcaggtt gctgtctttg
     9961 ccggtcaggc ctacggcctt gccaccagcc tgattgatca ggttgaccac atccttgttc
    10021 acctgtccgc cgagcaccat ctcgacgatc cccatcgtct cggcatcggt aacgcgcatg
    10081 ccctggatga actcgccctt cttgccgaca cgggtcagca aatcctcgat ctgcgggcca
    10141 ccgccgtgca cgacaacgac atgcatgccc accagcttca acagaacaac gtcacgggcg
    10201 aagccttgtt tgagtttctc gttgaccatc gcattgccgc caaatttgac gacgatggct
    10261 ttgccgtgga agcgcttgat gtagggtaac gcttcggcaa ggatggccgc ttcctgtgcc
    10321 ggtgagaggt gttgagaggt catggcaggg atccttgaaa tgactggcga ttctacccgg
    10381 cgacggggca aatataaaga cagtgaatag ccgtcctcgc aatggcctcg acttgcgtga
    10441 cgcgagccct gcggctagag tggcgcgatg agcgatcctg accttcagca agccttgcag
    10501 aaattgcgtc cgacgctgtt gcgcttcgcc atgctgcagc tgcgcgatga agcggcggcc
    10561 gaagatgtcg tacaggaaac gctgctcgcc gcactggaag ggaagagctc attttccgga
    10621 cgctcccagc tcaagacctg ggcggtctcg attctgagga acaagattgt ggatctcatg
    10681 cgacgtggtt cgcgcgaggg gaaactgccc ctggtcgacg aacacggcga tgaggacttc
    10741 gatgcgctct tcgatggttc cggtcattgg caggaaccgc caggcgactg gggcgatccg
    10801 gcagcagtgc tcgaacagcg tgatttctac gatgtgcttg aattatgcat gaaggccttg
    10861 ccggaaaatg tagcgcgggt gttcaccatg cgcgaggtgc tcggtttcga gactgaagaa
    10921 atttgtaagg aattgaagat gagtacgtct aactgttggg tagtgcttta tcgggcacgc
    10981 atgcggttgc gggaatgtct gcaattgcgc tggttcagcg tgaggtgacg agtatgaaat
    11041 cttgcaggga aattcaccgt ctggtgattg aagggcagga tcgcgagctc ggttttgccg
    11101 aacgtttttc catgcgcgtg catctgatga tttgcaccac ttgcaagcgt tttgatgcgc
    11161 agatcgatct gatgcggcaa gcgctaaggc gctttcccgg agattgaata cgaatatcgg
    11221 ggacctgtcc cgctgtccgc gctggcttga cggtgatgga tatccttacc gttccccact
    11281 ggcgctggtc gctggataac tggcttaagt agcgccaggc ccggtcaaca ctgaagtccg
    11341 aatttctgga gatgatccca tgctgatccc ccgccaagaa gtgcccgcgc tgaaggttga
    11401 aacgctatcc actggttcgt tcgacctggc gagcgaaggc ccggccaact tcactctgct
    11461 ggttttctat cggggtctgc attgcccgat ctgcgctaaa tatctgcttg agcttgcccg
    11521 tctgctgccg gattatgtgc aacgcggcgt tggcgtgatc gctgtgtcgt ccgatacgcg
    11581 cgagcgcgcg cttgagatgc agaagaagct tggtgttgcc gagcagcaat cgctgcgttt
    11641 tggctacggc cttcccctgg agaaagcgcg cgagtggggc ttgtatctgt cagcctcgcg
    11701 tggcaagact tcgatcggaa tcgaagagcc ggcattgttt gccgaacctg gattgttttt
    11761 ggtaaggccg gatcgcacgc tctattacgg cgcagtgcag accatgcctt tcgctcggcc
    11821 gcatttcgac gaactgctgc aggcaatcga tttcgcgatc gacaagaact acccggcgcg
    11881 cggtgaatat accggccctg gttagttaca agaaaagcaa tcgcaagctt accgagcctc
    11941 ccggctcggc gcggctg
//
LOCUS       DAORPM010000041        11820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1168_length_11820_cov_10.030089, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000041 DAORPM010000000
VERSION     DAORPM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11820
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1168_length_11820_cov_10.030089"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..184
                     /locus_tag="PLD53_03365"
     tRNA            108..184
                     /locus_tag="PLD53_03365"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:142..144,aa:Asp,seq:gtc)
     gene            complement(345..560)
                     /locus_tag="PLD53_03370"
     CDS             complement(345..560)
                     /locus_tag="PLD53_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59976.1"
                     /translation="MPDTSNRFCRRTGNLWESRYNSSLVQAETYLLACYRCIELDSVR
                     VGMVTEPGQYRWSSDASQVKAQADFGF"
     gene            619..747
                     /locus_tag="PLD53_03375"
     CDS             619..747
                     /locus_tag="PLD53_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59977.1"
                     /translation="MKRPSVAPAGFKQFVLEELAVFVTGEDCVAVVATQDDMLRYN"
     gene            1208..1492
                     /locus_tag="PLD53_03380"
     CDS             1208..1492
                     /locus_tag="PLD53_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRC59978.1"
                     /translation="MAVKVLIKRKIKNENLDALDFLLKKLRAATVNQKGYISGETYRR
                     FDEEGQSLVIGAWQNVDDWRKWFNSEERTEIQNQIDLLTDEPTEYEVYIP"
     gene            complement(1870..2046)
                     /locus_tag="PLD53_03385"
                     /pseudo
     CDS             complement(1870..2046)
                     /locus_tag="PLD53_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2261..2348)
                     /locus_tag="PLD53_03390"
     tRNA            complement(2261..2348)
                     /locus_tag="PLD53_03390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2312..2314),aa:Ser,seq:tga)
     gene            2489..3181
                     /locus_tag="PLD53_03395"
     CDS             2489..3181
                     /locus_tag="PLD53_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRC59979.1"
                     /translation="MQQQFRITGQATQVASLQQNKVLRNTYALLALSMIPTVIGALVG
                     VQMGFSFFAGSPMISFMVFLGVAFGFMWGIERTKNSGMGVVLLLGFTFFMGLMLSRIL
                     QVALGFSNGPTLIATAAGGTGAIFFTLAGIATVTKKDFSFLGKFLFIGVIVVLLAAVA
                     NIFFQIPALSLTISAVAVLVFSAYILYDISRIVNGGETNYISATLAVYLDVYNVFVSL
                     LNLLMAFSGERD"
     gene            complement(3317..4975)
                     /gene="recN"
                     /locus_tag="PLD53_03400"
     CDS             complement(3317..4975)
                     /gene="recN"
                     /locus_tag="PLD53_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRC59980.1"
                     /translation="MLHRLTIRDFVLVDRLELEFKPGFGALTGETGAGKSILVDALAF
                     ALGERSDSGLIRVGSERAEVSAEFDLRGSPAATDWLRGQDIDVEEGLLLRRVVDCTGR
                     SRAYLNGSPVTVQQLREVGESLVDIHGQHEHQSLLRGDAQRVLLDSHARLAPLKNEVS
                     DAWGQWRDAKMRLDSAASGVEELEREREQLEWQVREIESLGFSLEEWEPLNIEHKRLA
                     HAVSLVEGARFSLELLAEGEGACEVQVDSVARRLESLAEYDPALAEVAGLIQSAQAEL
                     AEAVSALRRYADRIDLDPQRLVDVERRIESVLGCARKFRSSPEALPALLEQLRERLTV
                     LRETADIAGLESRVNVARDHYEALAKRLSRSRAKAAEALGKEVSQVMQQLALGGGRFE
                     IGLVPVKGGAAFGLEQIEFRIAGFSGGEARPLAKVASGGELSRISLAIQVVTNQAVNV
                     PTLIFDEVDVGIGGGVAEVVGRLLSELGATRQVLCVTHLPQVAARASWQWQVCKLAED
                     GQIRSSVITLDGDARIEELARMLGGVEITTITRQHAREMLSLPD"
     gene            complement(5002..5928)
                     /locus_tag="PLD53_03405"
     CDS             complement(5002..5928)
                     /locus_tag="PLD53_03405"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRC59981.1"
                     /translation="MNEDPIRSPGGSPALPRTIALIGKYHSLEVADSLSILARNLDDR
                     GITVLVEEATANCSEASTDLKNWAIRDFAAIGEQADLAIVLGGDGTMLNAARQLARYR
                     VPLVGVNQGRLGFLTDIARCDMLTCMDELLSGKFTPENRMLLVAEVLRGGECIASSLA
                     LNDVVVDKGAIGRLIEFDLFIDNEFIYNLRSDGLITSTPTGSTAYSLSANGPILHPHV
                     SGIALVPLCPHSLTNRPILVGDRSEIEIRIVNATDSRVHFDGQVTIDLRPDDWVRIIR
                     SEFSIRLLQPPGYSYFAMLRQKLHWSEKPVTQ"
     gene            6016..7044
                     /gene="hrcA"
                     /locus_tag="PLD53_03410"
     CDS             6016..7044
                     /gene="hrcA"
                     /locus_tag="PLD53_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRC59982.1"
                     /translation="MLDERSRTLLKTLIERYIADGQPVGSRALSKFSGLDLSAATVRN
                     VMADLEDLGFISSPHTSAGRIPTSRGYRFFVDSLLVMHPLETERIHAIEGQLHPSQPQ
                     QLIISASHLLSGLTHFAGIVLTPRRKAPRIRQMEFVSLSEKRILLILVTTDGDVQNRI
                     LFTERPYSASDLVTATNYLNRYFSGHEFEHIRSRVKEDLRKLHGDLQDLMAAAMSASD
                     EALNREDEHYVISGERNLLGVEELSSNMKRLRELFDLFEQRTALMQLLDVSHRAEGVQ
                     IFIGGESGLAPLDECSVVTAPYEVDGQIVGSVGVIGPTRMAYEDVIPIVDITAKLLSS
                     ALTYQKNS"
     gene            7044..8147
                     /gene="hemH"
                     /locus_tag="PLD53_03415"
     CDS             7044..8147
                     /gene="hemH"
                     /locus_tag="PLD53_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRC59983.1"
                     /translation="MSRYLPEPEYRHGTAEKTAVLLINLGTPEAPTTSAVRRYLQEFL
                     SDPRVVEIPRAVWWLILNGIILNVRPRKSAEKYALIWTPEGSPLQVHTKRQATMLRGF
                     LGEAKHRVVVDYAMRYGQPSIPTTLSRLKTEGCTRFLLLPLYPQYSSSTTASAFDAAF
                     AWAASIRNQPELRTLRSYAGHPGYVEALASSVREHWMTNGRPHASYRLIMSFHGVPRY
                     TLDKGDPYHCECHKTARLLAEALNLGADEYQISFQSRFGRTEWLQPYTAQTLAALGKQ
                     GVQTVDVICPGFPADCLETLEEIAMEGKAEFIRAGGKAYRYIPCLNERDDWIHALADL
                     ASTHLQGWPTRDIPNTSALETSALRAKSLGAMS"
     gene            8243..9313
                     /locus_tag="PLD53_03420"
     CDS             8243..9313
                     /locus_tag="PLD53_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRC59984.1"
                     /translation="MKIDSASVLLDSSHSSLQRHELKESLRVWIGDRRPDLDGTQRRP
                     AGDVPPQVQVSDAGKTAQSSEVRAIQDGIDAAENDPKLLLIRAMIAMLTGRDVQVFDA
                     NELRVETPAAADQGQSRGSPTQAPPQAVDAAGFGVEYDRHESYTETEQTSFQATGVIR
                     TTDGKEISFNITLSMSRNYHEESDISIRAGDARKKLDPLVLNFNGSAAQLTSQRFKFD
                     LNSDGQAEEINFLSGNSGFLAFDRNGDGKINDGSELFGAKSGNGFLELSALDSDQNGW
                     IDENDAAYNQLSVWRKDGEGKDHLATLKQANVGAISLAHLDTPFDLKTGTNALLGQIR
                     SSGLFLQEDGKAGSVQQIDLTV"
     gene            complement(9351..10688)
                     /locus_tag="PLD53_03425"
     CDS             complement(9351..10688)
                     /locus_tag="PLD53_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRC59985.1"
                     /translation="MAEKIGKYEVIRTLGKGATAIVYLARDPDSDREVAVKLIRFGED
                     NAAMSRRLRKLFQTEDSIGRRLDHPNIVKIFDAVVEADRAYIVMEYIDGTVLEHYCSI
                     NRLLPMHRVVGIIFKCCLALDHAFRQGVVHRDIKPANILIDAEDNPKITDFGLALNLN
                     KDMNKDSTFVMGVGSPAYMSPEQVKNYPLNQKTDLYSLGVLLFQLLTGRLPFRATNQG
                     ALVYKIVNMDTPSVCDLNPNLPTGLDPILKKALEKDLYSRYRNGAEFAKDLSTVRYQM
                     LDDVDSTQDTTHFERLRNVEFFADFDDIELWEVLRISVWREVAPKVVLIREGDNNRIF
                     GVLVSGFVEVSQEGKSICRLGAGEVVGEMTFLHPSDTTRSASVTTLEPTLFLEINSAA
                     LELSSDEVQERFQKTLVAKVLGRLRGANQVLAKLGQPAVKGSRVGCDLELAPP"
     gene            10854..11396
                     /gene="grpE"
                     /locus_tag="PLD53_03430"
     CDS             10854..11396
                     /gene="grpE"
                     /locus_tag="PLD53_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRC59986.1"
                     /translation="MQAPDKETATEAGVENTEESTAPVVDSTPSLEETLRLAEQKAEE
                     HHDAWLRAKAESENVRRRAQEDIAKASKFAVERFAGELLAVKDSLEAALGAETPTIES
                     MKSGTELTLKQLVAAFEKSNLTELNPVGEKFDPHFHQAISMVDAEQEPNTVVTVLQKG
                     YLIADRVLRPALVIVSKPKS"
     gene            11474..>11820
                     /locus_tag="PLD53_03435"
     CDS             11474..>11820
                     /locus_tag="PLD53_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRC59987.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGGKPKVIENSEGARTTPSVVAYAE
                     DGEILCGAPAKRQAVTNPKNTLYAIKRLIGRRFEEKEVQKDIGLMPFKILKADNGDAW
                     VEVRNNKIAPPQVS"
BASE COUNT         2707 a         3496 c         3074 g         2543 t
ORIGIN      
        1 cactaccctt tcacggtagg taccggggtt cgaatccccg tggggacgcc aagaataggc
       61 aacggttgcc tggcatgaaa tgccgcaacc ctcgatctag aagtattgga gtggtagttc
      121 agttggttag aataccggcc tgtcacgccg ggggtcgcgg gttcgagtcc cgtccactcc
      181 gccaacattc cccaatggct gttttcgcga ccgaatccat cggacacgag agcagccatt
      241 ttgcgtttgc tgccgattac cgtgcgattt caatttgcat cggttccaat cgggtcagag
      301 acatttcgct tttccctgac cgtgttttcc tgttctgtcc ctgttcagaa cccaaaatcg
      361 gcttgtgcct tgacctgcga agcgtcgctg gaccagcggt attgaccggg ctcggttacc
      421 atgccgaccc gaaccgagtc gagctcgata cagcgatagc aggccagaag ataggtttca
      481 gcctgaacga ggctggaatt gtagcggctc tcccacaggt tgccagtgcg ccggcagaag
      541 cgatttgatg tatcgggcat agcgccgtcc caatgactgc atcagccggt gaacatagtt
      601 gctcatcagc acataggcat gaagcgcccg agcgtagcgc ctgccgggtt caagcagttc
      661 gtccttgaag aactggcagt cttcgtcact ggtgaagact gcgtcgcggt tgtggccact
      721 caggatgaca tgctgaggta caactgaact tgcggccgga gataagcgtg caaccaaatc
      781 cccgtggcct tcatcagaaa aacgccttgc aaacccggag gcgcccatct aattttagat
      841 atgcggttag gccacgctgg ttgccgtggc cgagccttct tcgcgctgtg cctgccgctt
      901 cgcgtcgcgc gtaagtctct cggccactcg ttccacgaaa gataatccga attttgggtt
      961 ctatactgag atcacggatc tttgaatgac tgatgtcatc tgcaagattc ggggtcgaac
     1021 acccgccgtc cttgtgacga ttcgctctgc gggaacactg agctttctgc ccccgttgaa
     1081 gccgtcgtca agacagggaa gggagaaatg ccgaggatgg caagagtagg aacctcggaa
     1141 tattgtttgt tgcccatttg cacgtgcaag tccgataaac agtaatcaac gcaaggaggc
     1201 acacatcatg gcagtcaagg ttctgatcaa acgcaagatc aagaatgaaa acctggatgc
     1261 actggatttc cttctcaaga aattgagagc agcgacagtc aaccagaaag gctacatctc
     1321 gggagaaacc taccggcgtt tcgatgagga aggccaaagt ctggtcatcg gcgcatggca
     1381 aaacgtcgac gactggcgca aatggttcaa cagcgaggag aggacggaaa tccagaacca
     1441 gatcgacctc ctgacggacg aaccgacgga gtacgaggtt tacataccct gacgcgcgac
     1501 acgcgggcaa gcgccccgga ttcagccatt ccgtcaattc gaagaccttg gctttccgcg
     1561 gcgtttggct gttccgattg agagatttcg gcccggatcg tgtgtcctga ttaaaacgtc
     1621 cggtaaagcc gggatacctc agcccgcgac tatcggccgc cttctccctc cgtacaagct
     1681 ccaaaccatt cttctcgcca ctcatccctt ttcttccacc accaccactt ccatccgcct
     1741 cgtatgctgg ctgtcacccc cgaagctgaa tgcctcatcg acctgcagcc ctctaggaat
     1801 ctgatgcgtg atgaagatca tcgtcacctt ccccttgaac ttgttgatcg tcttcgcaaa
     1861 gcgctccacc gtctgactgt cgagatttga gacgacccca tcgaagatca atatctttgg
     1921 ccacttgagc aaggctcggg caatggcgat gcgctgcttc tgcccacccg agagtccggt
     1981 gccgcgttcg ccgatctcgg tctggtagcc ttcgggcagt ttctcgatca cttcgtggat
     2041 ttcggctggc tcgtgcggct aagctcagac gacggctggg cggcttccat tccatttcaa
     2101 tgaaatggcc gcaagcgaac ggctggaaaa cgcacaaaaa ccagcgtcgg cgggacagcg
     2161 gcaaccagcc aatcgaggcg ccgattgata ttggaattga tggtcaacga ggcaattgaa
     2221 acaaaatatc caatacaatc atttgtttaa ttctgtgtat tggcggaagc ggtgagattc
     2281 gaactcacga aaggttgccc tttgccggtt ttcaagaccg gtgcaatcga ccactctgcc
     2341 acgcttcctt gttctggcca caatgctatc cgactgtggc ggagcgcatg ataacacgtt
     2401 acaaactgtt accccccgtc tcgcaacttg tggcatcctt gcgcagtctc aaacaagccg
     2461 caaacttgtt actttggagg atagatacat gcaacagcag tttcggatta caggtcaggc
     2521 aactcaagta gcatcactgc agcagaacaa ggtcctgcgc aatacctatg cgttgctcgc
     2581 tttgtccatg attccgacag tcatcggcgc actggttggc gtgcagatgg ggttctcgtt
     2641 ctttgccggc agcccgatga tttcctttat ggtgtttctg ggcgttgctt tcggtttcat
     2701 gtggggtatc gagcgtacca agaacagcgg gatgggggtt gtcctgctgc tgggcttcac
     2761 cttcttcatg ggtctgatgc tctcgcgcat cctgcaggtg gcgcttggct tttccaacgg
     2821 cccgacgctg atcgcgacgg cggccggtgg cacgggggcc attttcttta ccttggccgg
     2881 catcgcgacc gtgacgaaga aggatttcag tttcctcggc aaatttctct ttatcggcgt
     2941 gatcgttgta ctgctggccg cagtggccaa tatcttcttc cagatcccgg ccctatcgct
     3001 gaccatctcg gccgttgcgg tgctggtctt ttcggcatac atcctttacg acatcagccg
     3061 aattgtgaat ggcggagaga cgaactacat ctcggcaacg ctcgccgttt atctcgatgt
     3121 ctacaacgtc tttgtcagcc tgcttaatct gctgatggcg ttcagtggcg aacgcgactg
     3181 attcttgtgc atgacccggg cggcgctcag ccgtcgcccg atccttttct cctgctcccc
     3241 gcttctccct ctgatagctg gctttttcag tcccatccgc ttcgccggat gttttggcga
     3301 ctgaatcact gaccgctcaa tcgggaaggc tcagcatttc tctcgcgtgc tgccgggtga
     3361 tggtggttat ttcgacgccc ccgagcatgc gggcaagctc ttcgatcctg gcatcgccat
     3421 caagcgttat aacgctgctg cgaatctgac cgtcctcggc aagtttgcaa acctgccatt
     3481 gccaactggc gcgcgctgcg acttggggta gatgtgtcac acacaacact tgccttgtgg
     3541 cacccaattc actgagcaat cggcctacga cctcggcaac cccgccaccg attccgacat
     3601 ctacctcatc gaaaatcagc gttgggacgt tcaccgcctg attggtcacc acttgaatcg
     3661 ccagactgat tcgggaaagc tcgccgcccg aagcgacttt tgccaatggt cgcgcttcac
     3721 ctcccgaaaa gccggcaatg cgaaactcga tctgttccag tccaaaggca gcacctccct
     3781 tgaccggaac cagtccaatc tcgaatcgcc cacctccgag tgccagttgc tgcatgacct
     3841 ggctgacctc tttgcccaaa gcttcggcag ccttggcgcg gcttcttgac agccgttttg
     3901 ccaacgcctc atagtgatca cgggccacat tgactcgtga ttccagaccg gcgatatccg
     3961 cagtctcacg cagaacggtc agccgttccc gcaactgctc aagcagcgcc ggcaaggctt
     4021 ccggacttga gcggaatttg cgtgcgcagc cgagcacaga ctcaatccgt cgctcaacat
     4081 caaccaaacg ttgcggatcg agatcaatcc gatcggcgta tcggcgaagc gccgaaaccg
     4141 cttcggcaag ttccgcttgc gccgattgaa tcaggccagc cacttcggct agtgccggat
     4201 catattcagc caggctttca agccgtctcg caacgctatc aacctgcact tcacaagcgc
     4261 cctcgccttc ggccagaagt tccagcgaaa accgggctcc ctcgacaagg ctgacagcgt
     4321 gcgccaaacg cttgtgctca atgttcaggg gctcccactc ctcgagggag aaacccagcg
     4381 attcaatttc ccgaacttgc cactcaagct gttcgcgctc acgttccagc tcctcaacgc
     4441 cactcgctgc cgaatcaagc cgcatcttgg catcgcgcca ctggccccag gcatccgaaa
     4501 cctcgttctt cagcggagcc agccgggcgt gggaatcaag cagcacgcgt tgtgcatcgc
     4561 cgcgcagcaa cgattgatgt tcatgttgcc cgtgaatatc aaccagcgac tcgcccacct
     4621 cccgcaattg ctgaaccgta accggcgagc cgttcagata tgcgcgcgag cggcccgtgc
     4681 aatcaacgac acggcgcagc aacaatccct cttcgacatc gatatcctgt ccgcgcagcc
     4741 agtcggtcgc tgctggcgat ccgcgcagat cgaattcggc gctaacctcg gcacgttcgc
     4801 taccgacacg gatcaagcca ctatcggaac gctcgcccag agcaaacgcc agcgcgtcga
     4861 caaggatcga tttgccggcg ccggtttccc cggtcagcgc gccaaacccc ggcttgaact
     4921 caagctcaag acggtcaacc agcacgaaat cccgaattgt caatcgatgc agcatgggtg
     4981 ggcagagggc ctcagaatgg cctattgcgt tactggcttc tcgctccaat gcagcttctg
     5041 gcgaagcatg gcgaaatagc tgtaaccggg tggttgaagt aggcggatcg aaaattccga
     5101 gcgaatgatt cgcacccaat catccggcct caaatcgatc gttacctgac catcaaagtg
     5161 cacccgtgaa tccgtggcgt tgacgatgcg gatttcaatt tcactgcggt cgcccaccag
     5221 aatcggccga ttggtcagcg agtgcgggca aagcggcacc agggcaatcc cggaaacatg
     5281 gggatgcaga atcgggccat tggccgacag cgaataagct gtcgacccgg tcggcgtaga
     5341 cgtgatcagg ccatccgagc gcaggttgta aatgaactcg ttgtcgataa acaaatcaaa
     5401 ttcgatgagg cggccaatgg cgcctttatc aactacaacg tcattaagag cgaggctgga
     5461 cgcgatacat tcccctcccc gcagaacctc ggcgacgagc agcatacgat tttcaggtgt
     5521 gaattttccg ctgagcagct catccatgca ggtcagcatg tcgcagcgcg ctatatccgt
     5581 aaggaaaccg agtctcccct ggttcacgcc gacaagtggc acacgatagc gcgccagttg
     5641 ccgcgccgca ttgagcatcg ttccgtcgcc accaagaaca atagccaaat cggcctgctc
     5701 gcctattgcc gcaaaatcgc gaatcgccca gtttttcaaa tcggttgatg cttcggaaca
     5761 gttcgccgtc gcctcctcga cgagcaccgt aattccgcga tcatcgagat tccttgccag
     5821 aatgctgagc gaatcggcaa cctcaaggct gtgatacttt ccgatcagcg cgatggttct
     5881 cggcaaggcc gggctacccc cgggcgagcg aattgggtct tcattcatga atcgaattag
     5941 accacaattt gaatcgtttt cattgcacct tacgatcacc ccttttttct acgaatcttt
     6001 tgtagaatcg gtctcatgct cgacgaacgt tccagaaccc tgctcaaaac cttgatcgag
     6061 cgctatatag ccgacggcca gcctgttggc tcgcgcgcac tttcgaaatt ttcgggcctc
     6121 gacctgtcag ctgccaccgt acgtaacgtc atggccgatc tcgaagatct gggtttcatt
     6181 tccagccccc atacatctgc cggacgcata ccgacatctc gcggttaccg cttttttgtc
     6241 gacagcctgc tcgtcatgca cccgctggaa acagaaagga tccatgcgat cgaaggtcaa
     6301 ctccatccat cacaaccgca gcagctcatc atcagcgcat cgcacctgct ctccggactc
     6361 acgcattttg ccggcatcgt tctgacgcca cgccgcaagg cgcccagaat ccgacagatg
     6421 gagtttgtca gcctgtccga gaagcgcatc ctgctgattc tcgttaccac ggatggtgat
     6481 gtgcaaaacc ggattctgtt caccgagcgg ccctattcgg cctctgatct tgttactgca
     6541 accaactatc tcaatcggta cttctctggc cacgaattcg aacatatccg cagtcgggtc
     6601 aaggaagacc tgcgaaaact gcatggcgat ttgcaggact tgatggctgc ggccatgtcg
     6661 gcaagcgatg aggcattgaa ccgggaggat gagcattacg tcatttccgg cgagcgaaat
     6721 ctgctcggag tggaggaact ttcgtccaac atgaagcgcc tgcgcgaact tttcgatctc
     6781 ttcgaacagc gcaccgcgct gatgcaatta cttgacgttt cacaccgtgc cgaaggagtc
     6841 cagattttta ttggtggtga atcggggctc gcaccgctcg acgaatgcag cgtcgtcacc
     6901 gcaccctacg aggtcgatgg tcagatcgtc gggtcagtag gcgtcatcgg cccaacgcgg
     6961 atggcctatg aggacgtaat cccgatcgtc gatattacgg cgaagcttct ttcatccgcc
     7021 ctaacctacc agaaaaactc ctgatgtccc gatacctgcc tgagcctgaa taccggcatg
     7081 gtacggccga aaagacggcg gtgttgctga tcaatctggg tacgccggaa gccccgacca
     7141 cttcggccgt acgtcgctac ctccaggagt ttctctctga cccacgtgtg gtcgagattc
     7201 cccgggcagt ttggtggctg attctcaatg gcatcatcct caacgtccgc ccccgaaaat
     7261 cagccgaaaa atacgccttg atttggacac ctgaaggctc gccgttgcag gtgcacacga
     7321 aacgacaagc cacaatgttg cgcggtttcc tcggcgaagc caaacatcgc gtcgtcgtcg
     7381 actatgcgat gcgttacggc cagccatcga ttccgaccac gctctcccgt ctcaaaaccg
     7441 agggctgtac acgtttcctg ctgcttccgc tctacccgca atactcttca agcacgaccg
     7501 cgtcggcttt cgatgcagcc tttgcctggg cagcttcgat ccggaaccag ccggaactcc
     7561 gaacgctgag gagctatgcc ggccaccccg gatacgttga ggctctggcg agcagtgttc
     7621 gcgagcactg gatgaccaat ggccgcccac acgcatccta ccgcttgatc atgagttttc
     7681 acggtgtgcc tcgctacacc ctcgacaagg gtgaccccta tcattgtgaa tgccataaaa
     7741 ccgcccgtct gctggccgaa gcactgaatc ttggcgcgga cgagtaccag atcagttttc
     7801 agtcgcgttt cggtcgaacg gaatggttgc aaccgtatac cgcgcaaacg ctggctgcac
     7861 tcggtaaaca aggtgtgcag accgtcgatg tcatttgtcc cggctttcct gctgactgcc
     7921 tggaaacgct cgaagaaatc gccatggaag gcaaggccga attcattcgc gccggcggca
     7981 aggcgtaccg gtacattccc tgcctgaacg agcgcgatga ctggatccac gctcttgcgg
     8041 acttggccag tacgcacctg caaggctggc cgacccgaga tataccgaat acctctgctc
     8101 tcgaaaccag tgcactacgc gcgaaatcgc tgggcgccat gtcctgaggc acacaaaact
     8161 attcgtgatt cactctcaag tgacgacttc tcatagccgt taactcccta ccagcgagtt
     8221 aacggacgtc aaaggaggcc tcatgaaaat cgacagcgcc agtgtcctgc tcgattccag
     8281 tcattcaagt ctgcaacggc acgaactgaa agaaagcctg cgcgtgtgga tcggcgatcg
     8341 ccgcccggac cttgacggta cccagcgacg gccagcaggc gacgtaccgc cacaggtgca
     8401 agtctcagat gcgggaaaga ccgctcagtc cagcgaagtt cgcgcaattc aggacggcat
     8461 cgacgccgcc gaaaacgacc cgaagctgct cctcatccga gccatgatcg ccatgttgac
     8521 tggccgcgat gtccaggttt tcgacgcgaa cgaactgcga gttgaaacac cggcagcagc
     8581 agatcagggc cagagtcggg ggagcccaac ccaagcaccg ccgcaagccg tcgacgcggc
     8641 tggctttggt gttgaatatg atcgtcacga atcctacacg gaaaccgagc agaccagctt
     8701 ccaggcaact ggcgtcattc gtacgacaga tggcaaggaa atcagtttca acatcacctt
     8761 gtcaatgtcg cggaattacc atgaagaatc cgatatcagt atccgtgccg gagatgcacg
     8821 gaagaaactg gatccgctgg ttctcaactt taatggttca gccgcccagc tgaccagcca
     8881 gcgcttcaaa ttcgacctca attccgacgg tcaggcggaa gagataaatt tcctgtctgg
     8941 caacagcggt ttcctcgcat tcgaccgaaa cggcgatggc aaaatcaacg acggatcgga
     9001 actgttcggg gcgaagagcg gcaatggatt tctcgaactc tccgcacttg actctgacca
     9061 gaacggctgg atcgacgaaa acgatgctgc ctacaaccag ttgagcgttt ggcgcaaaga
     9121 tggagaaggc aaggaccatc tggcaacact gaagcaggcg aacgtaggtg cgatcagcct
     9181 cgcgcacctg gacacaccat ttgacctcaa aaccggcacc aacgccttgc tgggccagat
     9241 aaggtcaagt ggactcttct tgcaggaaga tggcaaagcc ggctcggtcc aacaaattga
     9301 tctgaccgta tgacgatcga catgtccatg catgctgatt tttcggacta ttaaggcggc
     9361 gccaactcca gatcgcagcc gacacgactt cctttcacgg ctggctggcc taatttggcg
     9421 agcacctgat ttgctccgcg caaacggccc aagaccttgg cgaccaaggt tttctggaat
     9481 cgctcctgaa cttcgtccga gctcaattcc agtgcggcac tattgatttc gagaaacagt
     9541 gtcggctcca aagttgtcac ggaagccgat cgcgttgtgt cgctcggatg cagaaaagtc
     9601 atctcaccaa ccacttcgcc tgcaccgaga cgacaaatgg acttgccttc ctgcgaaacc
     9661 tcgacgaaac cgctgacaag cacgccgaag attcggttgt tgtcgccctc gcgaatcaac
     9721 acgactttcg gtgcgacctc gcgccagacg ctgatacgta gcacttccca caattcgata
     9781 tcatcgaagt cggcgaagaa ttccacattg cgcagtctct cgaaatgcgt cgtgtcctga
     9841 gtggaatcaa catcgtcaag catctgatat ctgacggtgg agagatcctt ggcaaactcc
     9901 gcgccatttc gatacctgct gtaaagatcc ttttccagcg cctttttcag aatcggatcc
     9961 aatccggttg gcaggttcgg attgagatcg cacaccgaag gggtgtccat attgacaatc
    10021 ttgtagacca aggcgccttg atttgtggcg cgaaatggca accgcccggt cagcagctgg
    10081 aacaacagca ctccaagtga atacagatct gttttctggt tgagcggata gttcttgacc
    10141 tgttccggcg acatgtaagc cggggaaccc acacccatga cgaaggttga gtccttgttc
    10201 atgtccttgt tcagattcaa ggcgagtccg aaatcggtaa tcttcgggtt gtcttccgca
    10261 tcgatcaaga tattggccgg cttgatgtca cgatgcacga caccctgccg aaaggcatga
    10321 tcgagcgcca gacagcattt gaagatgatg ccgaccactc gatgcatggg caacaggcga
    10381 ttgatcgagc aatagtgttc aagtaccgtg ccgtcgatat attccatgac gatataggcc
    10441 cgatcagcct cgaccacggc atcaaaaatc ttgacgatat tgggatgatc cagacgccga
    10501 ccgatcgagt cctccgtctg aaagagtttg cgcagccgcc gcgacatggc cgcgttatcc
    10561 tcgccgaaac gaatcaactt gaccgcaacc tcgcgatcag aatccggatc acgagccaga
    10621 tagacgatgg cagttgcgcc cttgcccagg gttcgaatga cttcgtactt gccaattttt
    10681 tctgccatgc cgataccgtg tgaactcgtg ccaattcagg gtttgaatcg tagcagtgca
    10741 accctgcgaa agacgagatt atattaatcc gcgccaaaaa aacatgcttg cagtgcttga
    10801 aatctgaaaa tgcaccccca gctaggcctg agttttcagg gagaccacca tttatgcaag
    10861 cgcctgacaa agaaactgcg acggaagccg gtgtcgagaa cactgaggaa agcacagcgc
    10921 ccgtcgttga cagcacgcct agtcttgaag agaccctccg tctggccgaa cagaaggcgg
    10981 aagaacacca cgatgcctgg ttacgcgcca aggccgaatc cgaaaacgtt cgccgccgcg
    11041 cccaggaaga cattgccaag gcgtcaaaat ttgccgttga acgctttgcc ggtgagttgc
    11101 tggccgtcaa ggatagcctt gaagcggcgc ttggcgccga aacaccaacc atcgaaagca
    11161 tgaaatccgg caccgagctg acgctcaagc aactggttgc cgctttcgaa aaatcgaacc
    11221 tgacagagct taatccggtt ggcgagaagt tcgacccgca ttttcatcaa gcgatcagca
    11281 tggtcgacgc agaacaggag ccgaatacag tggtcaccgt cctgcaaaag ggctatctga
    11341 tcgccgaccg cgtattgcgc ccagctctgg tcatcgtttc caaaccaaaa tcttgaaaag
    11401 cactcaataa gccccaccta gggctcatcc cgacacatac aggtattcac gaatcacact
    11461 aaaggactct gacatgggca aaattattgg tatcgacctc ggcacaacca actcctgcgt
    11521 ttccgtcatg gaggggggca aaccgaaggt catcgaaaac tcagaaggtg cgcgcaccac
    11581 gccgtcggtc gtcgcttacg ccgaagacgg tgaaatcctc tgtggcgcgc cggccaagcg
    11641 ccaggcagtc accaacccga agaatacgct gtacgctatc aagcgcttga tcggccgtcg
    11701 cttcgaagaa aaggaagtcc aaaaggacat cggtttgatg cctttcaaga ttctcaaggc
    11761 tgacaacggc gatgcatggg ttgaagtgcg taataacaag atcgcaccgc cacaggtttc
//
LOCUS       DAORPM010000042        11815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1171_length_11815_cov_15.185459, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000042 DAORPM010000000
VERSION     DAORPM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_11815_cov_15.185459"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            511..963
                     /locus_tag="PLD53_03440"
     CDS             511..963
                     /locus_tag="PLD53_03440"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRC59988.1"
                     /translation="MNRLADAGLVRSERVGNLRRFQANPQSPVFSELVGLTRKTLGVE
                     PLLREALLPLAPGLQGAWVYGSVAKQTDTAQSDIDVMLVGENLLLGDVLELLVPLEAQ
                     LGRKINPTCYTPAEFERRRAEPDSFVNRVLAQPILPLIGDAHEPARAR"
     gene            941..1357
                     /locus_tag="PLD53_03445"
     CDS             941..1357
                     /locus_tag="PLD53_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59989.1"
                     /translation="MSLPALDNLVRIGQLKAEPRNEAEARRMLSMARTRLEDARLANL
                     SIEGRFTSAYNAAHAAALTALRWHGYRSENRYTVFQCLTHTLNWPAPRWRVLDAAHQK
                     RNLAEYEGFLEVDESAITELCDLAGSLIAEVSKMLE"
     gene            complement(1405..1809)
                     /locus_tag="PLD53_03450"
     CDS             complement(1405..1809)
                     /locus_tag="PLD53_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59990.1"
                     /translation="MRIFRAPAFLIVMVLSASSSAAEYSSIKQLLLAAIDASDGRASG
                     FILGQIAEKFKRTTGSSAPILAEVSTIKSFKQEGCKRLNVRLTQSGVATSEGRTADFG
                     MDYGLNLCRDGSPPTEGMDLEQAGKVLERQFD"
     gene            complement(1812..3419)
                     /locus_tag="PLD53_03455"
     CDS             complement(1812..3419)
                     /locus_tag="PLD53_03455"
                     /inference="COORDINATES: protein motif:HMM:NF009019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HRC59991.1"
                     /translation="MKTLALLLVLGVINSVFAAECRLSGSVCVDTSPSKNISGVPVTL
                     AEVGGCWEYEDTYTCIKPNAVNYCQPFVNAQPQCWQTFSQCAQMDTLFNSGCMKFTQT
                     WRCNDQTMPTPVNTIRLDDTYTLLSSDFDPGPCASLASNPSCQIAESTCISTTPPSLP
                     PGITTSQVAPDGCYQKQNVYACFTGRSDTSECDGYASNPNCTLESSSCNPADKINGQC
                     TFEQKTFRCMSQPGQTNTVTDCSGQLFCQNGNCFDKGYTGDTDFARSVALMEAAREAG
                     VYGAETEIFKGFDSRCRIKLFGLANCCKKSSGGGGFSNFNLLGSALGAGGQVLDVGSS
                     YVYDALYDGSTLQQGLGAALGVLGADGLFNPSFSFYGFSFQFLPSQGFIFTGFDPTTL
                     AIQIGVMILQDLLSCEQQEQILAMRSGQNLCHEIGTYCSSKIKLLSIKICIEHTKSYC
                     CYNSRLARILNEQGRGQIGKGWGGAKSPNCSGFTQAEFASIDFSSIDLSEFTAEIMAN
                     IKMPNVGTSSQQVQTSVQQKVQNYYQR"
     gene            complement(3416..4525)
                     /locus_tag="PLD53_03460"
     CDS             complement(3416..4525)
                     /locus_tag="PLD53_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59992.1"
                     /translation="MRRGIALAVLLLAPWLAMADQNGAFNDGKSLGTSLNQQNRDAIT
                     AGNIENKIPAYGQSAAEATYFQGGRGDTVGPGVNKVSTCSSVTPDSDPIKRQECEAVN
                     FLARNPDIRPQFSITGSDSMILGARSARANAESAFQSLGIAGGTGSSTQCQTRTETTP
                     AQFTTETCSSPREIGTEQCTTGRIINLDTDANFQCDQTMNAYENLQCRRGSTVTIGFG
                     QCTPGAWLGRAAYVDCSWCIDPYMAMNIFCGSDGRSYDVEPYRSYDGVNRYDYNWIGY
                     PWNGSYGLFHVPVAPGQSVTDYYVSNLGFGCNLYVYYSVSCSGTTCTPSMRNVSSGCN
                     SSGGTGVGSPLALPITRTVSNWTSDQCVALKLRAQ"
     gene            complement(4522..5250)
                     /gene="trbC"
                     /locus_tag="PLD53_03465"
     CDS             complement(4522..5250)
                     /gene="trbC"
                     /locus_tag="PLD53_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HRC59993.1"
                     /translation="MSIPTTFLPLALLLAASLSLAQGIFLEKDESNRLPTQGEIDLAR
                     QKTQEALNRLPDAPVIRQQFGGVTPRVEAMPRPATPAPDIASIAEKFKSLGRGLASQK
                     GAAPDLMVFVSLSMPREALARIIDQSERTGATLVFRGLKGDSMTRMGEEISALIGNRA
                     VSAAIHPPAFQQFTITRVPVVVIARPEAGSVMDDGCSKPDTFVKVTGDVTLDYALDYI
                     ERKSPDWAAIARSFRARLSGAAFQ"
     gene            complement(5268..6278)
                     /gene="traU"
                     /locus_tag="PLD53_03470"
     CDS             complement(5268..6278)
                     /gene="traU"
                     /locus_tag="PLD53_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HRC59994.1"
                     /translation="MKFIHSVLLLLATLLVSLPSHAATCKGKFANPITDICWSCMFPL
                     RIAGITLASLDQEDTPNPGGNPACFCGNPPKLGLKVSFWEPVRRVDVVRKPFCMVSLG
                     GVDIDPGFDAPVASRYRRDGQDQSSFYQVHWYVDPIIYYLQAVLDNSCLEQQGFDVAY
                     LTELDPLWKDDELTRLLNPDVYLFGNLPARAACAADCVTATAGFPNNTFFWCAGCQGN
                     LYPLNGNVQAHVGGVQASSLEVYRIIAKMHREGLMWAASGEDGLCGYYPQPVMDKTGY
                     KYHMLYPVPQTSKIAGKCCQPLGRSTILWGAGKEFPYEGEDFSYQIFRKRNCCQGAAT
                     LN"
     gene            complement(6262..6885)
                     /gene="traW"
                     /locus_tag="PLD53_03475"
     CDS             complement(6262..6885)
                     /gene="traW"
                     /locus_tag="PLD53_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HRC59995.1"
                     /translation="MRYAFLVTLPCLLLNPGFTLANDLGVVGPTYEIAERDLIEVMKD
                     KFRRMEKSGELARLQESYKQRVIETVEKPRPVHGVSSTETVRTFYVDPTWTLDRDVVD
                     EQGKLLFPAGTKVNPLDYAPLTQYLLFFDQREKTQVAFAKRFIEQSKARVKPILVGGE
                     PLKLMRQWKREVFYDQGGVLSRKFLLKQSPAIVSQEGKRLRIDEIHP"
     gene            complement(6954..9155)
                     /gene="mobH"
                     /locus_tag="PLD53_03480"
     CDS             complement(6954..9155)
                     /gene="mobH"
                     /locus_tag="PLD53_03480"
                     /inference="COORDINATES: protein motif:HMM:NF041494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HRC59996.1"
                     /translation="MFWRKRKSAPLPEPLRAGESIYRWGDDEIPRYPPFMKGLPVVSP
                     EKLLETQKELLDRISNSAIATPEIFQKHYLSAIKRFASFSHLLPASQSHHHRGAGGLL
                     RHAIEVGLWALQSADRVLLKSAISPKHRREMEPRWQLAVFLAALCHDAGKPVTDLTVT
                     DKDRTSTWHPITDDLHVWATRNGINAYFLEWREGRGRQHTALSSLIAERIIGVESLAW
                     IAEGETELVVWLMESLACNPGPTNLIYDLVIKADQTSVERDLKTLGVAMAGYDLGVPV
                     ERHLIDIMRRFVREGVWLVNQPGARLWNMEGHVYLVWPAAGEELARQIKEDGMPGMPR
                     TPEGILDMLTDRGMALVRMDAPSEDRYWKIAPAVLAEKIPDIGLTAIRLRDEAMVSSV
                     PLSSAAGRIINAETDASSLPASAVVEVQHAGAEIHSNTSSSAITESATTSRDSDPPQD
                     SSRPVTPPKSPPAITRNQEVRPEPGPGTEQMEAIKLDGAIGEALKALAQDIKARDKKW
                     GIDAIPDQDGHVYLRWPDAFAGYGLTPKTVLDQLADRGWLVIDPMSPLKRTIPKEFGD
                     GAHNAIRLGLEISKVFLLESGMSDGGEVSQAPEIQSHPQTTPPAGPVTARKSPDTQKA
                     EAKQRVKPSPALAPSTNGANHPGSTRDGQPANDTTKSAQAAPTIEALLAVLRSLDSAP
                     QQDGFCQITKMEAMRAFKRHGLKVNHRLLWKFDELKSGLLELMEDKIRFKP"
     gene            complement(9228..9725)
                     /locus_tag="PLD53_03485"
     CDS             complement(9228..9725)
                     /locus_tag="PLD53_03485"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRC59997.1"
                     /translation="MLNLIYRHLAKNAPGYLWLLAIGVLAGRYLAVSINVSDSLSGKV
                     YLIQKGVKPELGDLAAFRYQGGGPYAPGAWFLKRVTGIAGANVSSVDLGSGYVEFFVD
                     GKSVGRAKPKAKSGIPLMPGPTGTIPPRHYYMAAPHPDSLDSRYALVGWVDDEHVIGR
                     AFQIL"
     gene            complement(9834..10436)
                     /locus_tag="PLD53_03490"
     CDS             complement(9834..10436)
                     /locus_tag="PLD53_03490"
                     /inference="COORDINATES: protein motif:HMM:NF017124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FlhC family transcriptional regulator"
                     /protein_id="HRC59998.1"
                     /translation="MECAERQRIFHIKAGCTAQGATSISAEAASLPVYRTEAIGSSVV
                     VALAELENLQQAHELIRAGLRLSIIRGMTRIGTRTLRQWWKDVHGVKPSNGKLPETVL
                     SFIKDRDSAARLSAFAALHQRIFASDLTTTSLMTTWREYQRICGPLDINAAYFAARDV
                     RVKIVMLVQCQVCNAAFIYDAGSKHTDHCPFCETSVVVAD"
     gene            complement(10539..10862)
                     /locus_tag="PLD53_03495"
     CDS             complement(10539..10862)
                     /locus_tag="PLD53_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC59999.1"
                     /translation="MNDIISINRQFLIMAREASKSRSGEIMTGLSRTALDRIAQMTLD
                     EIDAMARDIGISVISIRLTETEMDRLLSLQGLQKAAYSISIAASARPRGGTGSFPTSR
                     QATEA"
     gene            10953..11393
                     /locus_tag="PLD53_03500"
     CDS             10953..11393
                     /locus_tag="PLD53_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60000.1"
                     /translation="MLLNSTPENDIADRLRLWRKDVLEMTQVRFAEATGVHLSAIRKY
                     EGRHSVPSGESLLAIASTGVDLHWLLTGKGDMRAPSNNGDSDAKTEADAELVRRLMAI
                     EGLLSGIQDDKRSAVLEEIFSRVQEAKRVDDLELLVGKLARKRA"
     gene            11518..>11815
                     /locus_tag="PLD53_03505"
     CDS             11518..>11815
                     /locus_tag="PLD53_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60001.1"
                     /translation="MSIRALEPNDASDHRSENAARLIRSYTARQGQYLAFIYYYSKIH
                     LCSPSEADMQRYFRVSPPAVHQMVLSLEKLGFIERTPGQGRSVKLLVPRDQLPDL"
BASE COUNT         2420 a         3438 c         3374 g         2583 t
ORIGIN      
        1 tcctggtgct tgccgcccgc ttgtgcgaga tcaatcgcgt gcccgaataa gtccttcacg
       61 tcctcaggca ttgaatccag atcgcgcttg gctgatccaa tccatttgag gggctttcga
      121 gcctgctttg acattcgctg cattatgccc atatgggcat atccttgtca atcgggcctg
      181 gaaggcgata ttgatccagt tcgttatcca tggcaggtgt ttccaagcat ccagactatc
      241 gggctgagat cctcgggatc aggacctcgc cagcaaatat aggctccact tcatgacatt
      301 tacatgcgtg actgtcgtga tcgcctgaag aactcatgct acttattggc atgttcatgc
      361 tattatttgg ctatgtcgat cgccacttct ctttttaccg acagccaatc ccgtgtcttt
      421 cagtggcttt ttggccagcc tgaacgtggt tatcacctga gcgaattgcg tcgccttacg
      481 ggcttgggaa gtgcctccct gcaaagagaa ttgaaccgac tggctgatgc gggtctggtc
      541 cgttccgagc gagtcggcaa cctgagacga ttccaggcca atccgcaaag cccggttttt
      601 agcgagttgg tcgggctgac gcgaaaaacg ctgggggtgg agcccctatt gcgcgaagca
      661 ctgctgcccc tggcccccgg cctgcaaggg gcatgggttt atggctccgt ggcgaaacaa
      721 accgacacgg cgcagagcga catagacgtg atgcttgttg gcgaaaatct gctacttggc
      781 gacgtcctgg aattgctggt cccactggaa gcgcaattgg gacgaaaaat caatccaacc
      841 tgctacaccc cggccgaatt tgagcgccgt cgtgccgaac ccgattcatt tgtgaatcgc
      901 gtcctggcgc agcccattct tccattgatt ggagatgccc atgagcctgc ccgcgctcga
      961 taatctggtc cgcatcggcc aactcaaggc cgaaccgcgc aacgaggccg aggccaggcg
     1021 gatgttgtcg atggcaagaa cccgcctgga agatgctcga ctggcaaatc tctccattga
     1081 aggtcgcttc accagcgcct acaacgccgc ccacgcggct gccttgaccg cccttcgctg
     1141 gcatggctat cgcagcgaaa accgatacac cgtgttccag tgcctcacgc ataccttgaa
     1201 ttggcctgcg ccccgctggc gagtgctgga tgccgctcat cagaagcgga atctggctga
     1261 gtacgaggga tttctggaag tagacgagtc cgccatcaca gagctatgtg atctggcagg
     1321 ctccctgata gcggaagtat cgaaaatgct tgaatagcca ggcccagact ccgattgcgc
     1381 gattggtccg tagcaataaa gccgttaatc aaactgtctc tcaagcacct tcccagcctg
     1441 ttcgaggtcc atcccttctg tcggcgggct gccatcccga caaaggttca aaccgtaatc
     1501 catcccgaag tcggcagtcc tgccttctga ggtcgcaacc ccgctttgag taaggcgcac
     1561 gttgaggcgc ttgcagcctt cctgtttgaa actcttgatc gtggatacct cggccaggat
     1621 tggcgccgat gatccggtcg ttctcttgaa tttttcggcg atctggccaa ggatgaaacc
     1681 actcgcccgg ccatcggaag catctatcgc tgcgaggagc aactgcttga tgctggagta
     1741 ctccgctgcc gagctgctgg ccgaaagcac catgacgatc aagaaggccg gggctctgaa
     1801 tatcctcata ctcacctctg gtagtagttc tggaccttct gctgaaccga ggtctgaact
     1861 tgttggcttg aggtgccgac gtttggcatc ttgatgttcg ccatgatttc ggctgtgaat
     1921 tcggacagat cgatactgga aaagtcgatg gaagcaaatt ctgcctgtgt gaaaccggag
     1981 caattcgggc ttttcgcgcc accccatccc ttgccaatct gcccccgccc ctgttcattg
     2041 aggattctcg ccagccgact gttgtagcag caatatgact tggtatgctc aatgcaaatc
     2101 ttgatggaca ggagcttgat cttgctggag caataggtcc ctatttcgtg gcagagattc
     2161 tgaccggagc gcatggcgag gatctgctcc tgctgctcgc atgacaagag gtcctgaaga
     2221 atcatcacgc caatctgaat tgccaatgtg gttggatcaa atcccgtgaa gatgaatccc
     2281 tggctgggta gaaattgaaa cgaaaaccca tagaagctga aggatggatt gaacagtcca
     2341 tcggctccca gcacacccag tgcagcgccc aacccctgct gcaatgtgga tccatcgtag
     2401 agggcgtcat aaacgtagga tgacccgaca tccagcactt gaccgccggc acccagcgcc
     2461 gagcccagca ggttgaaatt ggaaaaccct cccccgccac tgctcttctt gcagcaattg
     2521 gccaggccga acagtttgat ccggcatcgt gagtcaaagc ctttgaatat ttctgtttca
     2581 gcaccataga cgcccgcctc ccgtgcggct tccatcaagg ccacggatcg agcgaagtcc
     2641 gtgtctccgg tatagccctt gtcgaagcag ttgccgttct ggcagaaaag ttgccccgag
     2701 caatccgtga cggtgttggt ctggccaggt tgagacatgc aacggaaggt cttctgctcg
     2761 aatgtacatt gcccatttat cttgtcggcg ggattgcagg atgacgattc cagagtgcag
     2821 ttcgggtttg atgcgtagcc gtcacactcg gaggtatccg agcgtcccgt gaagcaggca
     2881 tacacgttct gcttctgata gcagccgtca ggagcgacct gggacgtggt tatgcctggt
     2941 ggcagagatg gaggggtggt cgagatgcac gtgctctcgg caatctggca acttgggtta
     3001 ctggccagcg acgcgcaggg ccctgggtcg aaatcactcg acagcagcgt ataggtatcg
     3061 tcaagccgga tcgtattgac tggcgttggc attgtctgat cgttgcatcg ccatgtttgc
     3121 gtgaatttca tgcacccgga attgaacagg gtgtccatct gcgcgcactg ggaaaatgtc
     3181 tgccagcact gaggctgtgc gttgacgaat ggctggcagt aattgacggc gtttggtttg
     3241 atgcaggtgt aggtatcctc atactcccag catccgccga cttccgccaa ggtgacggga
     3301 acgccggaaa tgttcttgct tggcgacgtg tcgacacaaa cggatcccga cagccggcac
     3361 tctgctgcga agacgctatt gatgacgccc aggaccagca ggagggcaag cgttttcatt
     3421 gagcgcgcaa tttcaatgcg acgcactgat ccgatgtcca gttgctgacc gtcctggtga
     3481 tcggcagtgc cagcggggag ccaacacccg tcccgccgga ggaattgcag ccggaactca
     3541 cgttgcgcat ggaaggagtg cacgtggtcc cgctgcaact cacgctgtag tacacgtaca
     3601 ggttgcagcc gaaccccaga ttgctgacgt agtaatcggt gaccgactga ccgggcgcta
     3661 cgggaacatg aaacagcccg tagctcccat tccaggggta tccaatccag ttgtagtcgt
     3721 aacgattcac cccgtcgtag gatcggtagg gctccacgtc ataggagcgg ccatcgctgc
     3781 cacaaaagat attcatggcc atgtagggat cgatgcacca gctgcaatcc acgtaagcag
     3841 cccggcccag ccatgcaccc ggcgtacatt ggccgaatcc gattgttacg gtacttccac
     3901 gccgacactg caggttctca taggcgttca tcgtctgatc gcattgaaag ttcgcatctg
     3961 tgtcgaggtt gatgatgcgc cccgtcgtgc attgctctgt cccaatttct ctcggagatg
     4021 agcaggtctc ggtggtgaac tgtgccggcg ttgtttccgt tctggtctgg cattgggtgc
     4081 tactgcccgt gcctccagca atgccaagcg actggaatgc gctctcggca ttggctcggg
     4141 cgctcctggc ccccagaatc atggagtcac ttccggtgat ggagaattga gggcgaatat
     4201 cagggttgcg cgcaaggaaa ttgacggcct cgcattcctg gcgcttgatg ggatcgctgt
     4261 caggcgtcac cgacgaacag gttgatacct tgttcactcc cggtcctacc gtatctcccc
     4321 tccccccctg aaaatacgtg gcctctgcag ccgactgccc atacgcggga atcttgttct
     4381 cgatgtttcc ggcagtgatg gcatctcgat tttgttgatt gagcgaggtg cccagcgatt
     4441 tcccgtcgtt gaacgcaccg ttttgatcgg ccattgccag ccacggcgcc agcaggagca
     4501 ctgcgagggc aattccccgt ctcactggaa tgccgctcca ctgagccgtg cccgaaagct
     4561 tcgcgctatc gctgcccagt ccggactctt gcgctcgata tagtccaggg cgtaatccag
     4621 cgtgacatcc ccggtgacct tgacgaaggt atccggcttt gagcagccat catccatgac
     4681 gctgcccgct tccggccggg cgatcaccac caccggcacc cgagtgatgg tgaattgctg
     4741 gaatgccggc ggatgaatgg cggcggacac cgcgcgattg ccgatgagcg cgctgatctc
     4801 ctcgcccatc cgggtcatcg agtccccctt caagccccga aataccagtg tcgctcccgt
     4861 gcgctcggac tgatcgatga tgcgagccag cgcctccctg ggcatggaga gcgagacgaa
     4921 taccatcaga tccggcgccg cccctttctg cgatgcgagt cctcggccca ggctcttgaa
     4981 tttctcggca atgctcgcga tgtccggggc cggcgttgcc gggcgcggca tcgcttcgac
     5041 tctgggggtg acaccaccaa attgctgccg gatcactggc gcatcgggca agcgattcag
     5101 tgcttcctgt gttttctggc gggcgagatc aatttcgccc tgggtcggca atcgattcga
     5161 ttcatccttt tccaaaaaaa tgccctgcgc cagcgacagg gatgccgcca gcagcagggc
     5221 caaggggagg aacgtggtgg gaatcgacat cgagggccta cgcatcttca attcagcgtc
     5281 gccgccccct gacaacaatt gcgcttgcga aagatctgat aggagaagtc ttccccttcg
     5341 tagggaaact ccttgccagc tccccacaga atcgtgctgc gccccagcgg ctggcagcac
     5401 ttcccggcga tcttcgatgt ctgcggcacc ggatagagca tgtggtactt gtagcccgtc
     5461 ttgtccatca ccggctgcgg gtaatagcca cacaggccgt cctcgcccga ggcggcccac
     5521 atcaggcctt cacgatgcat cttggcgatg atccgataga cctccagact ggatgcctgg
     5581 acgcccccca catgggcctg gacattgccg ttaagcggat agagatttcc ctgacagccg
     5641 gcacaccaga agaaggtgtt attgggaaaa ccggcggttg cggtgacgca atcggccgcg
     5701 cacgcggcac gggcaggaag gttgccaaac agatagacat cgggattcag aagccgggtg
     5761 agttcatcgt ccttccacag cgggtcgagt tcggtcaggt aggcgacatc aaagccttgc
     5821 tgctccaggc agctattgtc cagaaccgcc tgcaggtaat agatgatcgg gtcgacatac
     5881 caatgcacct gatagaacga ggactggtcc tgcccgtcac gccggtagcg gctggccacc
     5941 ggggcatcga accccggatc gatatcgaca ccaccgagac tcaccatgca aaaaggtttc
     6001 cggaccacat ccacccgacg taccggttcc cagaaactga ccttcaagcc gagcttgggg
     6061 ggattgccgc agaagcacgc cgggtttccg cctggattgg gggtgtcttc ctgatccagc
     6121 gatgccagcg tgataccggc gattcgcaag gggaacatgc aggaccagca gatatccgtg
     6181 atcgggttgg caaactttcc cttgcaggtt gcagcgtggg acggaagact cactagcaga
     6241 gtcgccagca gcaagaggac gctatggatg aatttcatcg atgcgcaacc tcttgccttc
     6301 ctgactgacg atcgccggag attgcttcag caggaatttc cggctgagca ccccgccctg
     6361 gtcgtagaaa acctcgcgct tccattggcg catcagcttg agcggctcgc ccccgacgag
     6421 aatgggtttc acccttgcct ttgactgttc gataaatcgc ttggcgaagg cgacctgagt
     6481 cttttcccgc tgatcgaaga acagcaggta ttgagtgagc ggtgcatagt cgagcggatt
     6541 gactttcgtt ccggccggga acaggagctt gccctgctcg tctacgacat ctctgtccag
     6601 cgtccaggtg ggatcgacgt agaacgtgcg gaccgtttcc gtgctgctga ctccgtggac
     6661 gggcctgggc ttttcaacgg tctcgatgac ccgctgcttg tagctctcct gcagcctggc
     6721 cagttcgccg ctcttctcca ttcgacgaaa tttgtccttc atgacttcaa tgagatctcg
     6781 ctccgcaatc tcgtaggtcg ggccgacgac gcccaggtca ttcgccaggg tgaaaccggg
     6841 gttgagcagc aggcacggca gggtcaccag gaacgcatat cgcatccgtc gatgatggca
     6901 gttacgtttt cggaatccgc cgccaaaagc acacactccg tgcgcttttc gaatcaaggc
     6961 ttgaatctga tcttgtcttc catcagttcc agcaaacccg acttgagctc gtcgaatttc
     7021 cagagcaggc gatgattgac tttcaggccg tggcgcttga acgctctcat ggcctccatc
     7081 ttggtaatct gacagaagcc atcttgctgg ggcgcactat caagggatcg aaggacggcc
     7141 aacagggcct cgatcgtcgg agcagcctgg gcggatttcg tggtgtcgtt tgctggctga
     7201 ccatctctgg tactcccggg atggtttgcc ccgttggttg atggcgcaag cgccgggctc
     7261 ggcttcacgc gctgtttcgc ctctgctttc tgggtgtctg gcgatttccg cgccgttact
     7321 ggccctgcag gcggcgtagt ctgcggatgc gactggattt ctggcgcttg gctgacttcg
     7381 ccgccatcgc tcattcccga ttcgagaaga aaaaccttgc tgatctcaag gcccaggcga
     7441 atggcgttat gagcaccatc cccgaattcc ttggggatgg ttcgtttcaa cggactcatg
     7501 gggtcgatta ccagccagcc ccggtcggcc aattggtcga gcaccgtttt tggagtcagc
     7561 ccatacccgg caaaggcatc gggccatcgg agataaacat gtccatcctg gtcaggaatg
     7621 gcatcaatgc cccatttctt gtctcttgcc ttgatgtcct gtgccagggc cttgagggct
     7681 tcgccaatgg cgccgtcgag cttgatcgct tccatctgct cggtacccgg ccccggctca
     7741 ggtcggacct cctgattgcg ggtgattgcc ggtggtgatt tcggcggagt gactggccgt
     7801 gaagaatctt gaggtggatc cgaatcgcga gaggtcgtcg ctgattcggt gatagcggag
     7861 gatgacgtgt tgctgtgaat ctccgcgcca gcgtgctgca cctcgaccac cgccgacgcc
     7921 ggcagactgc tggcatccgt ttccgcgttg atgatgcgcc ctgcagcaga cgaaaggggt
     7981 acggaggaca ccatggcctc gtcacgcaat cggatggccg tcagcccgat gtccgggatt
     8041 ttctccgcga gaacggcagg agcaattttc cagtaacgat cctcggacgg agcatccatc
     8101 cgaaccagtg ccattccacg atcggtcagc atgtccagaa tgccttccgg cgttcgcggc
     8161 attcccggca tgccatcctc cttgatctgg cgcgccaact cttcaccggc agcgggccac
     8221 accaggtaga catgtccctc catgttccac agtcgcgccc caggctgatt gacaagccac
     8281 accccctccc ggacaaacct gcgcatgatg tcgatcagat ggcgctctac gggcacaccc
     8341 aggtcgtagc cggccatggc taccccgagc gtcttgagat cgcgttccac gctcgtttga
     8401 tcggccttga tgaccaggtc gtagatgagg ttggtcggac cgggattgca ggccagggat
     8461 tccatcagcc agaccaccag ttcggtttcg ccttccgcga tccatgcgag ggactcaacc
     8521 ccgatgattc gctctgcaat gaggctggag agcgcggtgt gctggcgccc tcgcccctcg
     8581 cgccactcca ggaagtaggc gttgatgcca ttcctggttg cccaaacgtg gaggtcatcc
     8641 gtgatcggat gccaggtcga cgtgcgatcc ttgtcggtca ccgtcaaatc ggtgacaggt
     8701 ttgcccgcgt cgtggcacag ggcagcgaga aagaccgcca gctgccagcg cggctccatt
     8761 tcgcgtcggt gttttgggga aatggccgat ttcaacagaa cacgatccgc ggattgaagc
     8821 gcccacaagc ccacctcgat ggcatgccgc agcaagcccc cagctcctcg atgatggtgc
     8881 gactgcgacg ccggcaacag atgcgaaaac gaagcgaagc gctttatcgc cgacaggtaa
     8941 tgcttctgaa aaatctccgg cgtggcgatg gccgagttgg aaatcctgtc cagcagttcc
     9001 ttctgggtct caagcagctt ttccggggaa acgacgggca gccctttcat gaagggcgga
     9061 taacgtggaa tttcgtcatc gccccagcgg tagatggact cgccggcgcg taacggctca
     9121 ggcagaggtg ctgatttcct ttttcgccag aacatcagca ggcattcagg ccattgctgc
     9181 ggcttcgatt gctacggagg cttcagcgca agggctcgcg gggaacctca aagaatctgg
     9241 aaagcgcggc cgatgacgtg ctcatcatcg acccatccga cgagcgcata tctggaatcc
     9301 aggctatccg gatgcggtgc ggccatgtag tagtgcctcg gaggaattgt tcccgtgggt
     9361 ccgggcatca ggggaatgcc agacttcgct ttcggttttg cccgtccaac agattttccg
     9421 tcaacgaaaa attcaacgta tcccgacccc agatcgacac tactcacgtt cgccccggcg
     9481 atgcccgtga cgcgcttgag gaaccacgct ccaggcgcat agggaccccc gccctggtaa
     9541 cggaacgccg caagatctcc cagttcaggc ttgactccct tttgaatgag atagaccttg
     9601 cctgacaggg agtccgagac gttgatcgat acggcgaggt accgtccagc caggacacct
     9661 atggccagca gccaaagata accgggtgcg tttttggcaa ggtgccgata gatgaggttg
     9721 agcatcagag gatgctagcc atcgccccag ccaatttcag cccgataagc gcacttctcg
     9781 gcagcttttc aaaaaatgtc gatcacagcc tggcgtcgtt tgctccgaga cccttagtcg
     9841 gcgacaacca cgctggtttc gcaaaacggg cagtgatcgg tatgtttgct gccggcgtcg
     9901 tagatgaacg ccgcattgca cacctggcac tgcacgagca tgacgatttt tacccgcacg
     9961 tcacgagcgg caaagtaggc ggcgttgatg tccaggggac cgcaaatccg ctgatattcc
    10021 cgccatgtcg tcatcagact ggtcgtcgtc aagtctgagg caaagatgcg ctgatggagt
    10081 gcggcaaatg ccgacagtcg ggccgcagag tcacgatcct tgatgaagga cagtacggtc
    10141 tccggcaatt tcccgttcga gggttttacg ccgtgaacat ctttccacca ttgccgcaat
    10201 gttcgcgtcc cgatgcgtgt catgcctcga atgatggaca gtctcagccc ggcgcgaatg
    10261 agctcgtggg cctgttgaag attttcgagt tcagctaagg cgaccaccac cgaactccct
    10321 atcgcctcgg ttcgataaac gggaagactt gccgcttctg cggaaatgct cgtcgcgccc
    10381 tgagcagtac atcctgcttt gatgtgaaag atacgttgcc gttcggcgca ttccatggcc
    10441 gctgaccgcc aaggaagcca tcaaacgcga agttcgcctc tcggaacaaa gcgtcagggg
    10501 tgtgagcctg acaagtcctc acgctgaaat ccctggcctc atgcttcagt tgcctgtcgg
    10561 gatgtcggga aggaacctgt ccctccccgt ggtctggcag atgccgcaat agagatcgag
    10621 taggccgctt tctgcaagcc ctggagcgac agaagacgat ccatttcggt ttccgtcagc
    10681 ctgatgctga tgacgctgat gcctatgtct cgcgccatcg catcgatctc atccagagtc
    10741 atctgggcga tgcggtccag agcggttcgg gatagccccg tcatgatctc tccggacctg
    10801 gacttcgacg cttctctggc cattatgaga aactggcggt taatcgaaat gatgtcattc
    10861 atgtcgcttt tccaaaattg gactactatc cacggtgact aacgatatac atcgttcggt
    10921 ctgttgtcaa ttgtaaaaga aaccgaaaga ggatgctttt gaattcgaca cccgagaacg
    10981 acatcgccga tcggcttcga ctctggcgga aagatgtact ggaaatgacc caggtgaggt
    11041 tcgccgaggc caccggggtt catctcagcg cgatccgaaa gtacgagggc cgccatagcg
    11101 tgcccagcgg tgaatcatta ctcgccattg ccagcacggg ggtcgatctg cactggttgc
    11161 tcaccggtaa aggggatatg cgcgccccct cgaataatgg cgatagcgac gccaagaccg
    11221 aggcggacgc tgaactcgtt cgtcgtctga tggcgatcga gggacttctt agtggcatcc
    11281 aggacgacaa gcgatctgcc gtcctggaag agattttttc tcgcgttcag gaagcgaaac
    11341 gagtagacga cctggagttg ctggtaggca agttggcgcg gaaacgggca tagttcttcc
    11401 gctttcatac tactttggta ctggcaatat ccacttgaag aatgcatatt ccgaggaaaa
    11461 gcgacaccgg acccgatgcc agattgctcc cggcagccag ttccccagtt tgctgtgatg
    11521 agcattcgcg cgcttgaacc caatgacgca agcgaccacc ggagcgaaaa tgcggcgcga
    11581 cttatccgca gctataccgc aaggcagggg cagtacctcg ccttcatcta ctattactca
    11641 aagatccacc tgtgttcgcc gtcggaagca gacatgcagc ggtatttccg cgtgtcgccg
    11701 ccagcggtcc atcagatggt cctgagtctg gagaagctgg gattcatcga gaggacgcct
    11761 ggtcagggcc gctcggtcaa gctgctggtg ccccgcgatc agctgccgga tttga
//
LOCUS       DAORPM010000043        11736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1192_length_11736_cov_9.045801, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000043 DAORPM010000000
VERSION     DAORPM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1192_length_11736_cov_9.045801"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PLD53_03510"
     CDS             <1..142
                     /locus_tag="PLD53_03510"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRC60002.1"
                     /translation="LAALVTFWLAAKFKRWLGGYTGDCLGATQQLSEIAFYLGLLASL
                     PQ"
     gene            139..651
                     /gene="cobC"
                     /locus_tag="PLD53_03515"
     CDS             139..651
                     /gene="cobC"
                     /locus_tag="PLD53_03515"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HRC60003.1"
                     /translation="MILHLVRHPKPAIAPGICYGQLDISAENVAAVAIGLRDKLPPGL
                     PVWSSPLQRCRELASLLHPMPKIDDRLAEMNFGDWEGRPWDSIPRLEIDAWAADVAGY
                     VPPGGESALMLQQRALAFVNSLDLPEVVLVTHAGVIRTLLAHWQALPPAEWTQLIFAY
                     GSCTSIELPR"
     gene            689..1837
                     /locus_tag="PLD53_03520"
     CDS             689..1837
                     /locus_tag="PLD53_03520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC60004.1"
                     /translation="MKIAIIRQRYNPYGGAERFVERALGALVAEGTEVTLITRNWDGA
                     PREGFRTIICDPSPGSWLAGRAGRDKAFTACVQQVIAAGDFDLVQSHERVPGCSIFRA
                     GDGVHAAWLAQRARFLPPWRRLAQRFSPFHRHVLAAEEAMFSHPALRAVICNSHMVAD
                     EIVQYYGVDRSKLHVIYNGVDTHDFHPRLADLHRATVRQSLGVSGTTPLLLYVGSGYE
                     RKGVPQLLRAFVSAAKSGAHLLVVGADRKLNATRRLAERLGISESTHFLGPVKDVRPF
                     YGAADAFVLPTLYDPCPNAALEAMACGLPILTGIACGAREWVVEGQNGLIVDPLSENE
                     LARALEKLIRLSMEPAAHLLARQSVEALDLPTMSSRLLALYHRLSPQI"
     gene            complement(1914..3113)
                     /locus_tag="PLD53_03525"
     CDS             complement(1914..3113)
                     /locus_tag="PLD53_03525"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRC60005.1"
                     /translation="MIPANSWWRQPTFWNQALTVFVSLYILGLFLPGLPKLENIALYG
                     AVLITLFDGRWRSGLQWLTHPLILLLLALLGWLLLTATLSDQPLLSLTEYRRSFKDYF
                     LIFLPLMFVLSNEASRRLLARLIAFWGVIIVCTNGAQYVHEWFTDPSRLLDVKAHRGW
                     GHPLVFLLPFALMEMRLSAGRVSWAWLLLALVEAAMIIATGARGAWLAMLAVVLVWAV
                     AGFNRKQLLRLAAGGFVILLIAYFVLPTFLLRAKVEQGFDSSSRTTGTWGPAIEMMDE
                     RPMLGFGFGKDAFSREFNQRAPERESWSIKQSIGPHSLYLEAGFAGGYPALSGIILLF
                     GATLAYGYLGFGRSRLQEQRLFVLAASSAFVGFYLTRGAMETIRWGPMIILLGIIVSA
                     SRPPSSQ"
     gene            complement(3110..4195)
                     /locus_tag="PLD53_03530"
     CDS             complement(3110..4195)
                     /locus_tag="PLD53_03530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC60006.1"
                     /translation="MKIAIVNQHPTDMLGGSEIQCDIIARTLVEFGHEVDYIAVGGHD
                     CDAYEVPYRVRPVARQAKAIAEQVIAAKPDILYWRFNKHCFAKAAQRIKQAGIPIVFS
                     VSHVRDVSRFYLQPGTWRSGGLRGLRRALKESWQLYREHRGFDYVDALVSNNPDNLRG
                     ADIPLQAYIPNSMITTAQPFTWPRPYCLWVANIKDRKQPEKYVELASALPDLGVDFLM
                     VGQLQSKSYASMLANGPINFHYLGEKRLEEVNGMLAGSLFLIHTCHPEGFSNNFIQAW
                     LQGKTLVSLSFDPGGLLTNEGLGLYADNDMGNFIAQSRQLIESPDLANRMGEHARIYA
                     NSHFSPTTNVRQLESLFNELLKNPDCK"
     gene            complement(4192..5097)
                     /locus_tag="PLD53_03535"
     CDS             complement(4192..5097)
                     /locus_tag="PLD53_03535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRC60007.1"
                     /translation="MFFTVYTPTYNRADLLHRVYDSLVAQDDRDFEWLIIDDGSTDNT
                     ATIIENWAAKAPFPVRYVRQANQGKHIATNRAVELAAGEMFVIVDSDDWLTTGSLGAI
                     RQAWSSIPSAERDRFCGVAGLFIEPNGKACTKRFPEPHLDSNMVEIVTVHQIKGEISI
                     ATRTDIRRRFPFPENVGRFCMETLVWNRLAQHYLIRFTNQTFQIKDYQEQGLTKSGLA
                     RRMSTAPAAYIIRSQEFLGLQGMRIPWRQRQIAMRLLICASLHANIGFLQQLSETPHK
                     LLWLTQLRKGWREYRRHRATLDIAT"
     gene            complement(5100..6218)
                     /locus_tag="PLD53_03540"
     CDS             complement(5100..6218)
                     /locus_tag="PLD53_03540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC60008.1"
                     /translation="MNLLFAFPEPLPLPRARSVQVAWMTDALCAAGVNVTLAHVPSPD
                     GHPLQPIGKPLPARLRLLPLSNRWPFPFSRWHSVSRFTRQLVRHIDTASPDAIFVRHI
                     KLAYQLLKLRPNTPLIYEAHEVFAANAQPAKRQKLAEQEAFVLTHAKGIVHISRAVQE
                     ALHNDYLFSNQEIVLHSAAEIPKAPAIKDWANCARHIVYAGSFFSWKGVDDLIDAAAE
                     LPGYRMLLIGGEPAEIERLGARIAPTGAEVVLLPRMSSSQALEYLQEACIAVLPNRAE
                     GVSHFTSPLKLFEYMGAGCAVVAADLPSIREVLGDDEGRWFSPGSPAALAEAIRSLGE
                     APEIARSCGAAAAVKAGEYTWLGRAEALRRFIDSLLKV"
     gene            complement(6215..7330)
                     /locus_tag="PLD53_03545"
     CDS             complement(6215..7330)
                     /locus_tag="PLD53_03545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRC60009.1"
                     /translation="MSEPWLYVSDAKFSGIGIDLVSEQQILAFSESACPVDVVARGSL
                     KLPHVNNFRHPLPPTKLFSWLPSQDYYALNKRYFSHLGRRRFEQADYGGVVAWSRTAL
                     QIFEAAAKRGVPRLLNVGNSHRDFDSGLAANETPRWPRIPHTRYRAEYELATLILVAS
                     DQAAHSFHVQGVPPEKVRTIYRGADTERFRPLPDKPTRPFIVASCGLLGERKGTYQLL
                     GAWRRLALPDAELWLIGHLPEAEEQEVRAMASANVRFLGFRKDLPSLIRQAHLHVLLS
                     RNEGMAKVLLEAAASGVPNLCTPEAGLPPDAEGTLFISDRNCEDEVCAAIEACYLDTA
                     ATARLGLAARRMVEQRFGWAAFRSRFMQAVTEASRTK"
     gene            complement(7323..8240)
                     /locus_tag="PLD53_03550"
     CDS             complement(7323..8240)
                     /locus_tag="PLD53_03550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRC60010.1"
                     /translation="MAKNDSLVIFRKQLGDVLLLQPALALLAQQGKVSLATRANFADL
                     LALMPGNISLAPRWLPSASVVYCLEARRGALVYAAQALGATRHLLLTRDNAPWWQTLF
                     FTDMRIYPGETAYRAALYYQMVGGKAENFEPPHLKTPPPDWQLQGLPAAYGVIHPTAA
                     WQRKAWAPERWIEALAGLSDGLKWVISSGPAQWEKELASRLATGLGEQAINLAGRTSI
                     REYLALLANAKLTLCIDGSASHLSAAFGKPTLTLFGPTNPKHWHWASPITPYLSATDY
                     STEKRPPADAIPVAVVRKAIADLLEKIDV"
     gene            complement(8247..9008)
                     /locus_tag="PLD53_03555"
     CDS             complement(8247..9008)
                     /locus_tag="PLD53_03555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRC60011.1"
                     /translation="MAKLSVCLLTYNSSRTLERCLLPAQCIADELVIVDSGSTDGSLE
                     LLNRHGLSAIHRPYLTHADQMNFAIDQAAHDWVLCLDSDEFLDAETLAAITALKPELK
                     DPTIGYRIQRYWHVLGREVQAIYPVSSPDKPLRLFNRRQVRFNDQPVDDKAVGAKTRI
                     VLPGHVVHDTFYSLHELFSKLNSYTTRLVDLQNIPSSLTRAFISPIFAFLKWYFSKGA
                     WRDGGVGVVTGVYAAGYSFLKYFKAWCRHHRLPLK"
     gene            complement(9019..>10084)
                     /locus_tag="PLD53_03560"
     CDS             complement(9019..>10084)
                     /locus_tag="PLD53_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60012.1"
                     /translation="SLTMLGLMRRNRRPTPLRHLVSLLHRELPTLPVGPLAVQPPSHH
                     IFSAGTYTPVFARLAAETALKACPAEFRRDFRLYIHVDGVASSVRSGLLAWLREIPGV
                     EVTYGMFGILPFDRIPGKWHQVMVNDVVARFQGEKHLAFIDADLFVQDDSWWQQCRQH
                     LDDDVYALTVGLRPKSTLTLGEQHYVAMKTNLFTLNTQAHLELNRQRYTKDNRALELL
                     RREFPKGQLNVNAVDSLIGGSLRAQAHGYKVLDVEAEVPHCHIGGFSHLKAGKFKDYQ
                     NPERKDSVRGLLGQARFLPKVIAYFDHRGWGSFVEAGYRRDVGQMLAYIDSIAPLREM
                     MLSLPMTPRETMFETIVTSL"
     assembly_gap    10085..10184
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10185..11734)
                     /gene="msbA"
                     /locus_tag="PLD53_03565"
     CDS             complement(<10185..11734)
                     /gene="msbA"
                     /locus_tag="PLD53_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HRC60013.1"
                     /translation="MGANADKELSSKEVYWRLLGYVRPYRWAFIVAVCCMIVSSSLEP
                     ALPALLKFILDDGFAQNQDSLDWVFYPSLVFAIFLVRAIVGLIADYAMTWVSQHVIAD
                     LRKRMFDGLLRLPNSYFADNLSGRLISRVTSDVNGVASTATTAITTFIKDSFSIIGLL
                     GWLLYLNWKLTLITFAIAPLIAMAVRGFNGRVRSLARGLQGAQGMLTQVLQEAIEGQK
                     VIKIFGGQHYETQRFGKLIDNQRHLQMRSALALALQGPIVHFFVAVALAIIMAIALYQ
                     AGHGQATVGSFVSFITAMLMILAPLRRLVDVNATIQKGLIACESVFHVLDQPAETDHG
                     KHPFPRARGEIQFENVSFSYPGNDRNVLDDFSFTVKPGECVALVGPSGSGKTTIANLL
                     PRFFDVNSGQIRIDGVPIADIPLIDLRNNMALVSQDIVLFNDTIANNIAYGCSGSVSR
                     EAITTAAQAAHAMEFIERLPQGLDSLIGEKGAKLSGGQRQRLAIARALLKDAPILILD
                     EATSALDT"
BASE COUNT         2525 a         3087 c         3554 g         2470 t
ORIGIN      
        1 gctggcggcg cttgtcactt tctggctggc cgcgaaattc aagcgctggc tggggggtta
       61 caccggcgat tgcctggggg cgacgcagca gctcagcgaa atcgcatttt atctcggcct
      121 gctggccagc ttgccgcaat gatcctgcat ctggttcgcc accctaagcc ggcgattgcg
      181 ccgggcatct gctacggtca actcgatatt tcggccgaaa acgtcgccgc agtggcgatc
      241 ggcctgcgtg acaaattgcc gcctggtttg ccggtttgga gcagcccgct gcagcgttgc
      301 cgtgaactgg cgagcctgct gcatccgatg ccgaaaatag acgaccgttt ggccgagatg
      361 aatttcggcg attgggaagg tcgaccgtgg gattcgattc cgcgcctcga aatcgatgcc
      421 tgggcggccg atgtcgccgg ttatgtgccg ccggggggtg aatcagcctt gatgttgcag
      481 caacgagcgc tggcgttcgt gaatagcctt gacctgccgg aagtggtcct cgttacccat
      541 gccggtgtga ttcggacgtt gctggcccat tggcaggcgc tgccaccggc tgaatggacg
      601 caactgatat ttgcctacgg atcgtgcaca agcattgaac tcccccgtta aaagcacaac
      661 ggcaagcgct ggtactagaa tggcggccat gaaaattgcc attattcgcc agcgttacaa
      721 cccttacggc ggcgccgagc gctttgtcga gcgggcgctg ggcgctctgg ttgcggaggg
      781 tacggaagtt acgttgatta cccgtaattg ggatggggca ccgcgggaag gttttcggac
      841 gatcatctgt gacccttcgc ctggtagctg gctggccggg cgcgccgggc gcgacaaggc
      901 gtttactgcc tgcgttcaac aggtgattgc tgccggtgat tttgatctgg tgcagtcgca
      961 cgaaagagtg ccgggttgtt caatttttcg tgccggtgat ggtgtccatg cagcgtggtt
     1021 agctcaacgt gcccgatttt tgccaccctg gcgacgcctt gctcagcgtt tttcaccctt
     1081 tcatcgccac gtgctggcgg ccgaagaggc catgttctct catccggcac tgcgagccgt
     1141 gatctgcaat tcacacatgg tggcggacga gattgtgcag tattacgggg ttgaccgcag
     1201 caagctgcat gtcatctaca acggggtcga cacccacgat tttcacccac gcctcgccga
     1261 tctgcatcgg gcaaccgtac gtcaatcact cggcgtgtcc ggtacgacgc ccttgttgct
     1321 ctatgtcggc agcggttatg agcgcaaagg tgtgcctcaa ctattacgcg cctttgtcag
     1381 cgcggcaaag tctggcgcac atctgcttgt ggtgggcgct gatcgcaaac tgaatgcaac
     1441 ccgtcgtctg gcggagcgcc tgggtatttc tgaaagcacc catttccttg ggccagtcaa
     1501 ggatgttcga ccgttctacg gcgcggctga tgcgttcgtt ctcccgacct tgtacgaccc
     1561 ctgtccgaat gctgccctgg aagccatggc ctgcggtttg ccgatcctga ccggtatagc
     1621 gtgcggggca cgtgaatggg tggttgaggg ccagaatggt ctgatcgtcg atcctttatc
     1681 tgaaaatgaa ctcgcccggg cgttggagaa actgattcgc ctgtccatgg agccggctgc
     1741 tcatctactg gcccggcaaa gtgtcgaggc gctggatttg ccgacgatgt cgtcgcgtct
     1801 tcttgcgctg tatcaccgtc tctcacccca gatttgaccg agcacgaaca gagaggagat
     1861 tgctcccccg tcagtgctcg gcaaaactta ctgtgtttgg ttcgagagat tgatcattgg
     1921 ctggagggcg gacgggatgc gctgacgatt atcccgagca ggatgatcat tgggccccag
     1981 cgtatcgtct ccatagcgcc gcgggtgagg tagaaaccga cgaatgcact gcttgcagcc
     2041 aagacaaaaa ggcgctgttc ttgaagtctg cttcgcccaa agccaaggta accgtatgca
     2101 agtgttgccc caaacagcag gataatgcca gacagtgcgg gatagccacc cgcaaatcca
     2161 gcctcaagat aaagactgtg cggccctatc gactgtttga tgctccatga ttcgcgttcc
     2221 ggcgcgcgct ggttgaattc ccgactaaaa gcgtccttgc cgaaaccgaa gcccagcatg
     2281 gggcgctcat ccatcatctc gatggccggt ccccaggtgc cagtggtgcg tgaagaggag
     2341 tcgaatccct gctcaacctt ggcccggagg aggaaggtgg gcagcacgaa gtaggcaatt
     2401 agcaggatga cgaagccacc agccgcaagc cgaagcaatt gcttgcggtt gaaaccggca
     2461 actgcccaga ccagaacgac tgcaagcatg gccaaccatg ccccgcgtgc gccggtcgca
     2521 atgatcatgg cagcctcgac aagcgccagc agcaaccatg cccaggagac gcggccagca
     2581 ctcaggcgca tttccatcag cgcaaagggc agcaggaaaa ccagtggatg cccccagcct
     2641 cggtgggcct tgacatccag caggcgcgac ggatcggtaa accattcgtg cacgtactga
     2701 gcaccatttg tgcagacgat gatcacaccc cagaaagcga tgaggcgggc gagcagacga
     2761 cggctggcct cattgctcag cacgaacatc aggggtagaa agatcagaaa atagtccttg
     2821 aaactgcgcc ggtattcggt gagtgacagc aagggctgat cggatagcgt tgccgtgagc
     2881 aacaaccaac cgagcagcgc gagcagcaac agaatcagcg gatgggtcag ccattggagg
     2941 cccgatcgcc agcgaccatc gaacagggtg atcagaacgg caccgtacaa cgcgatattt
     3001 tcgagcttgg gcaggccggg cagaaaaagg ccgaggatgt atagggaaac gaacacggtg
     3061 agtgcctggt tccagaacgt tggctgtcgc caccacgaat ttgctgggat catttgcagt
     3121 ccggattttt gaggagttcg ttgaataagg attccagttg acggacgttg gttgtcggcg
     3181 agaagtggct gtttgcataa atacgggcgt gttcgcccat acggttggca agatccggtg
     3241 attcaatcaa ctggcggctt tgggcgatga agttccccat gtcgttgtcg gcatacagac
     3301 cgaggccttc gttcgtcagt aatccacccg ggtcaaaaga caggctgacc agggttttac
     3361 cctgcaacca ggcctggata aagttgttgc tgaatccttc tggatgacag gtgtggatca
     3421 aaaacaggct accggcaagc atgccgttaa cttcttccag acgtttctcg ccaagataat
     3481 gaaaattgat cgggccgtta gcaagcattg aagcgtaaga cttgctctgc aactggccaa
     3541 ccatcagaaa gtccacgccg agatcaggca gtgcgcttgc caattcgaca tacttttcag
     3601 gttgcttgcg atccttgatg ttggcgaccc ataggcagta cggacgtggc cacgtgaacg
     3661 gctgcgcggt ggtgatcatc gagttcggaa tgtaggcctg cagcggaatg tctgccccac
     3721 ggaggttgtc cgggttgttg ctgaccaaag catcaacata gtcaaagccg cgatgctccc
     3781 gataaagttg ccagctttct ttaagtgcgc gtcgcaggcc gcgcagcccc cctgaacgcc
     3841 aggtgccggg ctgcagataa aagcgggaaa catcgcgcac atgagaaaca gagaagacga
     3901 tcgggatacc ggcttgtttg attcgttgtg ccgccttggc gaaacagtgt ttgttgaatc
     3961 gccagtaaag tatgtctggc ttggctgcaa tcacctgttc ggcaatcgct ttggcctgtc
     4021 gagcgaccgg gcgaacacga taggggactt cgtaagcatc gcagtcgtgg ccaccgactg
     4081 cgatgtaatc gacctcatga ccaaattcga cgagcgtgcg tgcgattatg tcgcactgaa
     4141 tttcactgcc acccagcata tccgtcgggt gctgattgac gatggcaatc ttcatgtcgc
     4201 gatatccagc gtggcgcgat ggcgtcggta ctcacgccat cctttgcgca attgggtgag
     4261 ccaaagaagt ttgtgaggcg tctcggaaag ctgctgtaaa aaaccgatat tggcatgcag
     4321 cgaggcacag attagcagcc gcatcgcgat ttgccgttgc cgccagggaa tgcgcatccc
     4381 ctgcaagccg agaaattcct gactgcggat aatgtaagcg gcgggggctg tgctcatgcg
     4441 gcgggccaag ccggatttgg tcaggccttg ttcctgataa tccttgatct ggaacgtttg
     4501 gttggtaaag cggatcaggt aatgttgcgc taagcggttc cacaccaagg tttccatgca
     4561 gaagcgcccg acattttctg ggaagggaaa acgccggcga atatcggttc gcgtcgcgat
     4621 cgaaatttcg ccttttattt ggtgcaccgt gacgatctcg accatattcg agtcgagatg
     4681 aggctctggg aagcgcttgg tgcatgcttt gccgtttggt tcgataaaga ggcccgcaac
     4741 gccgcagaag cgatcccgtt cagccgaggg gattgatgac caggcttgcc ggattgcgcc
     4801 aagtgaacct gtagtgagcc agtcgtccga gtcgacgatg acaaacattt cgcctgcggc
     4861 aagttcgact gcacggttgg tggcgatgtg ctttccctga ttggcctgcc ggacataacg
     4921 taccgggaat ggggccttcg ctgcccaatt ttcaatgatc gttgctgtgt tgtcggtcga
     4981 gccgtcatca ataatcagcc attcaaagtc acggtcatcc tgtgccacca ggctgtcata
     5041 aacacggtgc aacaaatctg cgcgattgta ggtcggcgta tagacggtaa agaacatgct
     5101 cagactttca gcagggaatc gataaaacga cgtagtgcct cggcccgtcc cagccaggtg
     5161 tattccccgg ctttaacggc agctgctgca ccacagctgc gggcgatttc aggcgcttca
     5221 cccaggctgc gaattgcttc ggcgagggcg gccggggagc caggggaaaa ccagcggcct
     5281 tcatcgtcac ccaagacttc gcgtatcgac ggcaggtctg ccgcgacaac ggcacaaccg
     5341 gcacccatgt attcaaacaa tttcagtggc gaggtgaaat ggctgacgcc ctcggcccgg
     5401 ttaggtaaca ctgcgatgca ggcttcctgc aggtattcca aggcttgcga tgagctcatg
     5461 cgcgggagca agaccacttc ggcgccggta ggcgcgatcc gggcgcccag tcgctctatt
     5521 tcggcaggct ctccgccgat caatagcatg cgatagcccg gtagttcggc ggcggcatca
     5581 atcagatcgt caacaccttt ccagctgaag aagctcccgg cgtaaacaat gtgccgggca
     5641 cagttggccc agtctttgat cgccggcgct ttgggaattt cggctgcgct gtgcaggact
     5701 atttcctggt tactgaatag gtagtcgtta tgcaaggcct cctgaacggc acgggaaata
     5761 tgcacaatac ccttcgcgtg ggtcagtaca aacgcttcct gctcagcgag tttctggcgt
     5821 ttggccggct gcgcgttggc ggcgaaaact tcatgtgcct cgtaaatcag tggggtgtta
     5881 ggacgtaatt tgagtagttg ataggccagt ttgatgtgcc ggacgaaaat agcatcgggt
     5941 gaagcagtgt cgatgtgccg gacaagctgg cgggtaaaac gggataccga atgccaacgg
     6001 gagaacggaa acggccagcg gttagatagt ggtagcaggc gcagccgggc aggcaggggt
     6061 ttgccaatgg gttgcagcgg atgaccatcg ggcgagggaa cgtgagccag cgtcacgtta
     6121 accccggcag cacatagcgc atcggtcatc caggcgacct gaacactgcg cgcacgcggt
     6181 aagggcagtg gttccgggaa agcgaacagg aggttcattt cgtccttgaa gcctctgtga
     6241 cggcctgcat gaaacggctt cggaacgctg cccagccgaa gcgctgttca accatacgac
     6301 gggccgcaag gccaaggcga gcagtggcag cagtgtcgag gtagcaagct tctatcgccg
     6361 cacacacttc atcttcgcaa ttgcgatcac tgataaacag ggttccctct gcatccggcg
     6421 gtaagcccgc ttcaggtgta cataagttcg gtacgccact ggccgctgct tcaagcagca
     6481 ccttggccat tccctcgttg cgtgacagca gcacgtgtag atgtgcctgc cgtatcaggg
     6541 atggcagatc cttacggaat cccagaaaac gcacgttggc gcttgccatc gcacgtactt
     6601 cttgctcttc ggcttcgggg agatggccaa ttaaccagag ttcggcatca ggtagggcca
     6661 atcgccgcca agcaccgagt aattgatagg tacccttgcg ttcgccaagc aggccgcaac
     6721 tggcgacgat gaatgggcgg gttggcttgt cgggtagcgg tctgaagcgc tcggtgtcgg
     6781 cgccacgata tatggtgcga accttctcgg gagggacgcc ctgaacgtgg aagctgtgtg
     6841 ccgcctgatc tgaggccacg agaatcaagg tggcgagttc gtattcagcg cggtagcgag
     6901 tgtgtggaat gcgtggccag cgtggggttt cgttagccgc gaggccacta tcgaaatccc
     6961 ggtgcgaatt gccgacgttc agcagtcgtg gtactccccg cttggctgct gcttcaaata
     7021 tctgcaacgc tgtgcgggac caggcgacga ctccgccata gtctgcctgt tcgaagcggc
     7081 gacggcccaa gtgggaaaag tagcgcttat tcagcgcata gtagtcctgc gatggtagcc
     7141 aggagaataa tttggtcggc ggcaagggat gccgaaaatt atttacgtgc gggagtttga
     7201 ggctgccgcg ggcgacgaca tcgacggggc aagcagactc ggaaaaggcg agaatctgct
     7261 gttcgctgac caggtcgata ccaattccgg agaatttggc gtcactaacg taaagccaag
     7321 gttcagacat caattttttc cagcaagtca gcgatcgcct tgcgtaccac agcaactgga
     7381 atggcgtcgg cggggggacg cttttcagtg ctgtagtcgg tggcgctcaa gtagggagtg
     7441 atggggcttg cccaatgcca atgcttcgga ttggttggcc cgaacagggt tagcgttggt
     7501 tttccgaagg cagcagagag atggctggct gaaccgtcta tgcacaaggt aagttttgcg
     7561 ttggcaagca aagcgaggta ttcgcgtatg gatgtccgtc cggccagatt gattgcctgt
     7621 tcgcccaaac ctgttgcgag ccgggatgcg agttccttct cccattgcgc ggggccgctg
     7681 ctgattaccc attttaatcc gtcactcaat ccggcaaggg cctcgatcca gcgctccggc
     7741 gcccaagcct tgcgctgcca agcggccgtg ggatgaataa cgccataagc cgcgggaagt
     7801 ccttgcaatt gccagtcagg aggtggagtt ttcagatgag gtggctcgaa attctcagct
     7861 ttgccgccga ccatctgata atacagcgct gcacgataag cagtttcgcc tggataaatc
     7921 cgcatatcgg tgaaaaaaag agtctgccac cagggcgcgt tatcacgagt cagtagcagg
     7981 tggcgggttg ccccgagggc ttgagccgcg tagaccagag caccacggcg tgcttccaag
     8041 cagtaaacga cgcttgccga cggtagccag cgtggtgcta acgaaatgtt tccaggcatc
     8101 agcgccagca gatcggcgaa gtttgcgcgc gtcgccagcg agaccttgcc ttgctgggcc
     8161 agcaaggcaa gggcgggctg caataagagc acatcgccaa gttgcttgcg gaagatgacg
     8221 aggctgtcgt ttttcgccat ggatgcctat ttgaggggta aacgatggtg ccgacaccaa
     8281 gctttgaagt acttcaaaaa tgagtaacca gcggcgtaaa cgcctgtcac aacaccgaca
     8341 ccgccatccc gccaggcccc cttgctgaaa taccatttca ggaaggcgaa gataggtgat
     8401 atgaaggcgc gggtaaggct cgatggtatg ttctggagat cgaccaagcg ggtggtatag
     8461 ctattgagct tgctgaataa ctcgtgcaat gaatagaagg tgtcatgaac gacgtgtccg
     8521 ggcagcacaa tccgtgtttt tgcaccgacg gccttgtcgt cgacgggttg gtcgttgaaa
     8581 cgtacctggc gccggttgaa taggcgcagt ggcttgtccg gcgaagaaac cgggtagata
     8641 gcctggactt cacgaccaag gacatgccag taacgctgga tgcggtagcc gatcgtcgga
     8701 tccttgagtt ctggcttcag tgccgtgatg gcggcaagcg tctcagcatc gaggaactca
     8761 tcgctatcga ggcagagcac ccagtcgtgt gcagcttggt cgatggcaaa gttcatctgg
     8821 tctgcatgcg tcagataggg gcggtgaatc gctgacaggc cgtgccgatt tagcaactca
     8881 aggctgccat cagtactgcc agaatcgacg atgaccaatt cgtctgcgat gcactgcgcc
     8941 ggtaacaggc agcgctcaag cgtgcgggaa gagttgtagg tcagtaggca gacggacagt
     9001 tttgccatga gagccgtctc agagactggt gacgatggtc tcgaacatcg tttcgcgtgg
     9061 cgtcataggg aggctcagca tcatctcgcg cagcggcgct atcgagtcga tataggctag
     9121 catctggccg acatcgcggc ggtagcccgc ctccacgaaa ctaccccagc cacggtgatc
     9181 gaaataggcg ataactttgg gcagaaagcg ggcctgaccc agtaggccgc gtacggaatc
     9241 cttccgctcg ggattctggt agtccttgaa cttgcctgcc ttgaggtgtg agaatcctcc
     9301 gatgtggcag tgaggaacct ccgcctcgac atccagtacc ttgtagccat gagcctgggc
     9361 acgcagggag ccgccaatca ggctgtctac agcgttgacg ttgagctggc ccttggggaa
     9421 ttcacgacgg agcaactcca gggcccgatt gtccttcgta taacgttggc ggtttagttc
     9481 gagatgggcc tgggtgttca gcgtaaagag gttggtcttc attgccacgt agtgctgctc
     9541 gccgagggtc agcgtactct tggggcgcaa gcccaccgtc aaggcataaa cgtcgtcatc
     9601 gagatgctgg cgacactgtt gccaccagct atcgtcctgc acgaaaaggt ctgcgtcgat
     9661 gaaagccaga tgcttttcac cctggaagcg ggcgaccacg tcattgacca tgacttgatg
     9721 ccattttccg ggaatccggt cgaaggggag gatgccaaac atgccgtagg tgacttccac
     9781 gccgggaatt tcgcgtagcc aggcgagcag gcccgagcgt accgatgagg caacaccatc
     9841 gacgtggatg taaagtctga aatcgcggcg aaattcggcc gggcatgcct tgagcgccgt
     9901 ttctgcggcc agtcgtgcga agactggcgt gtaggtgccc gccgaaaaga tatggtgcga
     9961 gggaggttgt accgctaatg gcccaacggg aagcgtgggg agttcccgat gcaacaggct
    10021 gaccaggtga cgtagtggcg tggggcggcg attacgccgc attagtccga gcatggttaa
    10081 gcttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcggtg tccagcgcgg
    10201 aggttgcctc atcaagaatg aggatcgggg cgtctttgag caaggcacgg gcgattgcca
    10261 ggcgctgacg ctgcccacca gacagctttg ctcctttttc accaatcagc gaatcaaggc
    10321 cttgcggcag ccgttcgatg aactccatgg catgggccgc ctgggcggcg gtggtaatcg
    10381 cttcccggct gaccgagcca ctgcagccgt aggcaatgtt gttggcgatc gtgtcgttga
    10441 acaacacaat atcctgactg acgagtgcca tattgttgcg caagtcgatc agtgggatat
    10501 cggcgatggg tacgccatca atacgtatct ggcccgagtt gacatcgaag aagcggggca
    10561 gcaaattggc aatggttgtc tttccactgc ctgagggtcc gaccagcgcg acgcattcac
    10621 ctggtttgac cgtgaagctg aaatcgtcga ggacgtttcg gtcgttaccc ggataactga
    10681 agctgacgtt ttcaaactgg atttcgcccc gggcacgagg gaaagggtgt tttccgtggt
    10741 ctgtttcggc gggttggtca agaacatgaa agacactttc gcaggcgatc aggcccttct
    10801 ggatcgtcgc attgacatcg accaagcggc gtagcggtgc caggatcatc agcattgctg
    10861 tgatgaagga tacgaagctg cctacggtgg cctgaccgtg gcctgcctgg taaagcgcaa
    10921 tcgccatgat gatcgccagg gccacggcaa cgaagaaatg gactattggg ccttgaaggg
    10981 cgagcgccag cgctgagcgc atttgcaggt gacgctggtt atcgatgagt ttgccaaatc
    11041 gttgcgtttc gtagtgctgt ccaccgaata ttttgatgac cttctgacct tcaatggctt
    11101 cctgcagtac ctgagtcaac atgccttgag caccctgcag gccacgcgcc aggctacgga
    11161 cccgaccatt gaaaccacgt acagccatgg caatgagcgg cgcaattgca aaagtaatca
    11221 gggttagctt ccagtttagg tagaggagcc agccgagaag accgatgatg ctgaaactgt
    11281 ccttgatgaa ggtggtgatg gctgtcgttg ccgtgcttgc tacgccgttg acgtcactgg
    11341 tgacacggga aatcaggcgt cccgagaggt tgtctgcgaa gtaactgttg ggtaggcgta
    11401 acaggccatc aaacatccgt ttgcgcagat cggcaatgac gtgctgggag acccatgtca
    11461 ttgcatagtc ggcgatcaag ccgacgattg cccgaaccag aaagatggcg aatacaagag
    11521 aggggtagaa cacccaatct aacgagtcct ggttttgtgc gaagccatca tcaaggatga
    11581 acttcaacaa ggcaggcagg gcgggctcta gtgaggacga cacaatcatg cagcagactg
    11641 cgacgatgaa ggcccagcga taagggcgca cgtaacccaa aaggcgccag taaacttcct
    11701 tgctgcttaa ttccttgtcg gcgtttgcgc ccatga
//
LOCUS       DAORPM010000044        11690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1198_length_11690_cov_9.965793, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000044 DAORPM010000000
VERSION     DAORPM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1198_length_11690_cov_9.965793"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLD53_03570"
     CDS             <1..390
                     /locus_tag="PLD53_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HRC60014.1"
                     /translation="DQDSAPDCRVVKAGLMERCHHAGQAEKTLVRIACKELETFYLAD
                     LMAVEQAMGLTSLASQQAAAKFRNPDRLGSPSRELEVLTRGKYQKVTGSRIIGKYLDP
                     ANKRSSSFKHLLNGIRHMEGELLNNMG"
     gene            534..1190
                     /locus_tag="PLD53_03575"
     CDS             534..1190
                     /locus_tag="PLD53_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRC60015.1"
                     /translation="MRFRILAVLAVFVSLLSGCGYNTFQTGDEAVKSAWSEVVNQYQR
                     RADLVPNLVSTVQGYASHEKDVLTQVTQARAQVGGMQVTPELLNNPEAFAKFQQAQAG
                     LSGALSRLLVVAENYPNLKADASFRDLQAQLEGTENRITVARNRYIKSVEAYNVSVRT
                     FPNNLTAMIFGYQVKPNFSVENERAISVAPKVDFSASVPQTPVSAAVEGGKSPQTPGY
                     "
     gene            1194..2078
                     /locus_tag="PLD53_03580"
     CDS             1194..2078
                     /locus_tag="PLD53_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HRC60016.1"
                     /translation="MIVPSLRRSEWLAGLFLLFVAGWVAAQTLQPIPALSSRVTDLTA
                     TLSADQRSSLEEKLAAFERQKGAQVAVLIVPTVKPETIPEYALRVVESWKLGRKGVDD
                     GVLLLIAKEDRKLRIEVGYGLEGALNDATAKRIISETISPRFKQGDFYGGIDSGLETM
                     LRVIGGESLPEPEQQRDAKNSDDGLDTLLLVGFVLVFVVGGIVRAIFGRFLAAGIIGG
                     VVGIVASLLLSSMLIAIVVGVIAFLVSLFNGMGGGGGRGGGGGWSSGASSWGGSSGGG
                     GFSGGGGSFGGGGASGDW"
     gene            2078..2584
                     /locus_tag="PLD53_03585"
     CDS             2078..2584
                     /locus_tag="PLD53_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRC60017.1"
                     /translation="MWKATILRTLHHLVVPDWWALRSFPKSTRLRIERAIAASESAHL
                     GELRFVVEASQSLHGLLHDQSARARAVELFSQLGVWDTEHNSGVLIYVQLVDRRVEIV
                     ADRGINARVEEGFWNAICRRIEAAYRAGSFEQGTLLALNDVTAVLAEHFPATGDNPDE
                     LPNAPVIR"
     gene            complement(2621..3883)
                     /gene="lplT"
                     /locus_tag="PLD53_03590"
     CDS             complement(2621..3883)
                     /gene="lplT"
                     /locus_tag="PLD53_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HRC60018.1"
                     /translation="MPLGFYLIMAAQFFSALADNALLIVAIAALREMDSPSSYEPLLK
                     TFFTVSYVALAAFVGAFADSMPKWRVMFISNTIKIFGCGLMLLTVHPLLAYAVVGLGA
                     AAYSPAKYGILTEYLPHRLLVVANGWIEGLTVGAIILGVVIGGTLIQPAIAKHLLSLN
                     FPFFDTGIDTTNEMALCIVGGLYILASIFNLYIPDTGVDHKALHKNPWYLLREFNHCL
                     ALLWRDRLGQISLAVTTLFWGAGATLQFIVIKWAEVALHLDLSKSSMLQGAVALGVAL
                     GAIGAAKMITLRKSLKVIPLGIAMGLIVLIMNFVHYVWLAIPLLVIIGALSGFFVVPM
                     NALLQHRGHILMGAGHSIAVQNFNENLSILVMTGLYYLMIKLDLSIYLVITLFGLFVT
                     TSMVLVKRRHEANQREYDDVIHLDDGTN"
     gene            4055..5149
                     /gene="alr"
                     /locus_tag="PLD53_03595"
     CDS             4055..5149
                     /gene="alr"
                     /locus_tag="PLD53_03595"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRC60019.1"
                     /translation="MSRPIEARIDLAALRHNYLIAKSHAALQGRPAKAWAVVKADAYG
                     HGLMRAAKALDGVADGFALLDLADAVALRDSGFRQPILLLEGLFEPDEVTVCAEYGLI
                     PVVHRIEQLQMIRAAALPLRLPIYLKLNTGMNRLGFTLEQLPAVRRELAVSGAIGAVT
                     LMTHFAEADGGRGIDFQLERFETMRQGWPDTSPVEISLANSAAILRYPQTAHDWVRPG
                     IMLYGGCPFAESDAASFNLKPVMTLHSRILAVQEIKPGERVGYGGVFEATRPTRVGII
                     ACGYADGYPRHAPGGTPILVAGQRTETLGRVSMDMLACDLTELPDAGVDSPVVLWGEG
                     LPADEVAAAAGTISYELFCALARRVPVVEV"
     gene            5160..6524
                     /gene="radA"
                     /locus_tag="PLD53_03600"
     CDS             5160..6524
                     /gene="radA"
                     /locus_tag="PLD53_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRC60020.1"
                     /translation="MAREKTIYACTECGASAPKWQGQCPDCGVWNTLVETIAESTPAN
                     KSRFAALSGASRLQTLSEIVANEEDRLPTGIGEFDRALGGGLVSGGVVLLGGDPGIGK
                     STLLLQALSELSGGRHVLYVSGEESGQQIAMRARRLALDTKQLQLLTEINLEKILALL
                     RLEKPQVAVIDSIQTLWSDQLSSAPGSVAQVRECAAQLTRLAKQTGVTVILVGHVTKD
                     GALAGPRVLEHMVDTVLYFEGDTHSSFRLIRAVKNRYGAVNEIGVFAMTDRGLKGVSN
                     PSAMFLSQHGQEVPGSCVLVTQEGTRPLLVEIQALVDQSHGNPRRLTVGLDAQRLAML
                     LAVLHRHAGIVCFDQDVFVNAVGGVKISEPAADLSVLLAIVSSLKNKVLPRKLIVFGE
                     VGLAGEIRPAPRGQERLKEAVKLGFTRAMIPEANKPKHPIAGIEVIAVRRVDEAMDRL
                     RELD"
     gene            6533..9064
                     /locus_tag="PLD53_03605"
     CDS             6533..9064
                     /locus_tag="PLD53_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60021.1"
                     /translation="MSTVAFALRMLRRDLRAGELHLLVAALVVAVAALTAVGFFADRV
                     RQSLEREANQLLGADLLLTSDHLWPPNVAADARGRGLAVVETRTFPSMVTLGEGDSLR
                     AHLAEIKAVSEGYPLRGSLRIAPGRNVADSPASGIPQAGTIWIDERLASSLSAKVGDT
                     ISVGMLALRVAAILTLEPDRGLNFFSVAPRLLLNIADLEATGLIQPGSRITYRLLLAG
                     EATLVAEFRGRVEASLQRGERIEDAQNGRPEIRTALARAQTFLGLSALLTVVLAAVAI
                     ALASRRYMQRHLDPCAVMRCLGATQGFLLGVFLGQFAWLGLFSAVLGCVIGYLAHFVL
                     HAWLAQLLATPLPLPGLLPALQGSVIGVLLLFGFSAPPLIQLKRVSTLRVLRREFSSG
                     SIPPSRLLAGYAVGFAALAGLMFWIAGEVRLGAYVVGGFSVAMLLFALVARVVIRFAA
                     LLRGGARPGARQGGIGWRYGLASLERHHAASVVQIVALALAFMALLLLTVTRNDILDA
                     WRQALPPEAPNRFVVNIQPDQLDSVQGAFAEDQLSAEFSPMVRGRLARVNDVEVGPSS
                     YDDERAKRLIDREFNLSYRMDLPEGNEVTAGRWFSEADEGRGAASVEVGLAATLGLKP
                     GDRLEFVIAGEQVTMQVIGLRKLNWDSMRVNFFVLTPPAVLKPYPASWITSFYLAPEK
                     ADFVNRLIAEFPNLTVVDVAAILQQLQSVMNRVAQAVQFIFLFTLMAGIIVLYAALAS
                     AADERRYELAVMRALGARREQLRAALLAEFATIGGLAGLIASLGAIAVGQLLAEMVFK
                     FEVAIDISLPFAAMICGAGLVTGAGWFAASRLMNVPPFEALRSGG"
     gene            complement(9047..9958)
                     /gene="prmB"
                     /locus_tag="PLD53_03610"
     CDS             complement(9047..9958)
                     /gene="prmB"
                     /locus_tag="PLD53_03610"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRC60022.1"
                     /translation="MFDQAQNELSTLRDLMRFAVSRFIESELFFGHGNDNAWDEAAYL
                     LLHTLKLPLDRLDPFMDARLTSDERAAVLKVIKRRIAARLPAAYLTNEAWLGDYRFYV
                     DERVIVPRSHIAELLREQLSPWIDDPWAVTRVLDLCTGSGCLAILAAHAFPESKVDAL
                     DLSPDALAVAKRNVEDYGLGSRLRLVQSNAFAGVQGQSYDLIISNPPYVNAESMDSLP
                     DEYRREPELALAGGKDGLDFVRILLKEAAGHLNPNGLLVVEIGHNRDDLEKAFPETLF
                     TWLDTSAGDRFVFLLRREELPGLAAGS"
     gene            complement(9960..11093)
                     /gene="dapE"
                     /locus_tag="PLD53_03615"
     CDS             complement(9960..11093)
                     /gene="dapE"
                     /locus_tag="PLD53_03615"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRC60023.1"
                     /translation="MPGDATLKLAEQLIACRSVTPEDGGCMRIIGERLERMGFGCEYI
                     NRGGVTNLWARRGSASPLFCFAGHTDVVPTGPLDQWQSDPFQPTRRDGLLYGRGAADM
                     KSSLAAFVTATEAFVASRPEHSGSIAFLLTSDEEGDAVDGTVAVTEALAVRGEKMDFC
                     VVGEPTAVSRLGDTIKNGRRGSLTGKLTVRGVQGHIAYPHLAKNPIHLVAPAMTELVN
                     CIWDQGNASFPPTTWQISNIHGGTGANNVIPGNVLIQFNFRFATTSTPEELKARAGEI
                     LDRHGLDYEIDWTLGAKPFLTGGGALVDASLKAIREETGIEAELSTTGGTSDGRFIAD
                     LCPQIIEIGPVNASIHKIDECIEIAALPRLSAIYRRILEQLLP"
     gene            complement(11095..>11690)
                     /locus_tag="PLD53_03620"
     CDS             complement(11095..>11690)
                     /locus_tag="PLD53_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRC60024.1"
                     /translation="DCILIGEIRDKETMQAAIAYAQTGHLCLATLHANNSYHALNRVI
                     NFFPLETRTSLYLDLSASLKAIVSQRLVKKPDGKRTPAAEILLNTRHVQELIERGEVL
                     AVKEAMEQSLAPGSQTFEQDLFRLYRENTITLDEALGNADSPTNLSWLINNSEITSGT
                     QNEELRVETPLDFSETSDSGASFKEFSLSLNDAPEET"
BASE COUNT         2220 a         3158 c         3771 g         2541 t
ORIGIN      
        1 gatcaagaca gtgctccgga ttgtcgggtg gtaaaggccg ggttgatgga acgctgccat
       61 cacgctgggc aagccgagaa aaccctagtg cggattgctt gtaaggagct tgagacattt
      121 tacttggccg atctgatggc tgtagaacag gccatggggc tgacgagtct tgccagtcag
      181 caggcggctg cgaagtttcg caatcccgat cgccttggaa gcccaagtag ggagcttgag
      241 gttctcacac gggggaaata ccagaaagtg accggatcgc gcatcattgg aaagtatcta
      301 gaccctgcga acaagcgctc gtccagcttc aagcatttgc tcaatggcat ccggcatatg
      361 gaaggcgaat tgctcaacaa tatgggataa atcgatgtgt ttgcaaatgc tcctgagata
      421 ttcgtcgatt ggccgtcatt tagaagcacc cagtcagata ctcctcttgt aattcaatgt
      481 atcaacgcta tttacgttcc gctttgttcc attttttcgt ggagaaaact gtcatgcgct
      541 tcagaattct tgccgtcctc gccgtctttg tcagtctgct ttccggctgt ggatacaaca
      601 ctttccagac gggcgatgag gcggtcaaat cggcgtggtc ggaagtcgtc aatcagtacc
      661 agcggcgagc cgatctggtg cccaatctgg tcagtaccgt acagggctac gccagccatg
      721 agaaagacgt tctgacgcag gtcacacagg ctcgcgccca ggttggcgga atgcaggtga
      781 cgccggagct actcaacaat ccggaggcat ttgccaagtt tcagcaggca caggccggcc
      841 tttcgggggc gctgtcccgg ttgctggtgg tcgcggaaaa ctacccgaac ctgaaggcgg
      901 acgcctcgtt ccgcgacttg caggcacagc ttgaaggtac cgagaaccgc atcaccgtgg
      961 cgcgcaaccg ctacatcaaa tcggttgagg cctacaacgt ctcggttcgc acattcccga
     1021 acaacctgac ggcaatgatt ttcggctacc aggtcaagcc caacttcagc gtcgagaacg
     1081 aacgagcgat ttcagtagcg ccgaaagtcg acttctcggc gtcggtaccg cagacgcccg
     1141 tgtctgctgc ggtcgaaggt ggcaagagcc cccagacgcc cggatactga tccgtgatcg
     1201 ttccaagcct caggcggtcg gagtggctag ccgggctgtt cctgctcttt gtggcggggt
     1261 gggtcgcggc gcagacgctg cagccgatcc ccgcgctttc ctctcgggta actgatctta
     1321 cggcgactct atctgcagat cagcgcagca gtctggaaga gaaactggcc gcttttgaac
     1381 gacagaaagg cgcgcaagtt gccgtactga tcgtgccgac cgtcaagccg gaaactatcc
     1441 cggaatatgc gctgcgtgtc gtcgaatcgt ggaagctcgg acgcaagggc gttgatgacg
     1501 gcgttttgct gctgatcgcc aaggaagaca ggaagcttcg catcgaagtg ggctacggac
     1561 tggaaggggc gctgaacgac gcgacagcca aacgcatcat cagcgaaacg atcagcccgc
     1621 gcttcaagca gggtgacttc tacggcggta tagattccgg cctggagaca atgctcaggg
     1681 taattggagg agaatccctc ccagagccag aacaacagcg cgatgcgaag aactccgacg
     1741 acggtcttga tacgctgctc ctcgttgggt tcgtgctcgt tttcgtcgtt ggcggtatcg
     1801 tgcgcgccat cttcggccgt tttctggctg caggaatcat tggtggcgtg gtaggcatcg
     1861 tggcgtcgct gctgctatct tcgatgctga ttgccattgt ggttggcgtt attgccttcc
     1921 tggtttcgct gttcaacggc atgggcggcg gaggcgggcg tggaggaggg ggtggctggt
     1981 cgtcaggcgc ctcgtcctgg ggtggcagtt cgggaggtgg cgggttttcg ggtggtggcg
     2041 gaagctttgg tggcggtggc gcctcgggag actggtaatg tggaaagcca cgattctcag
     2101 aaccctgcat catcttgtgg tgcccgactg gtgggcgttg cggtcatttc caaagtcgac
     2161 gcggctcagg atcgaacggg ccatcgccgc atccgagagc gcgcacctgg gcgaattgcg
     2221 ctttgtcgtt gaagccagcc agtcgttgca cgggctgctt cacgaccaat cggcccgcgc
     2281 acgggcggtc gagttgtttt cgcaacttgg cgtctgggac accgaacaca atagtggcgt
     2341 gctgatctac gtgcaattgg ttgatcgccg tgtcgagatc gtggctgatc gcggaatcaa
     2401 tgccagggtt gaggaagggt tctggaacgc gatttgccgg cgtattgagg cggcttaccg
     2461 ggcaggcagt ttcgaacagg ggacgctgct tgccttgaac gatgtcaccg ccgtgcttgc
     2521 cgaacacttc ccggcgaccg gcgataaccc tgatgaactg cccaatgcgc cagtgatccg
     2581 ttgatacgtt gcatcgcgtg ttttcaggca gtgcgctggt tcaattcgtg ccgtcatcga
     2641 ggtggatcac atcgtcgtat tcccgctggt tggcctcatg ccggcgcttt acgagaacca
     2701 tgctggtggt aacgaagagt ccgaacagcg tgataaccaa gtaaatcgaa aggtcgagct
     2761 tgatcatgag gtagtagagg ccggtcatga cgagtatcga caggttttcg ttgaaattct
     2821 gtacggcaat cgaatggccg gcgcccatca ggatatggcc gcggtgctgc agcagtgcgt
     2881 tcatcggcac gacaaagaag ccggagaggg ctccgatgat gaccagtagc ggaatggcca
     2941 gccacacgta gtggacgaaa ttcatgatca ggacgatgag gcccatggcg atgcccagcg
     3001 ggatcacttt gagcgacttg cgcagcgtaa tcatcttggc ggcaccgatg gcgccgagcg
     3061 ccacgccaag tgcgacggca ccctgcagca tgctcgattt ggacagatcg aggtgcaggg
     3121 ctacttctgc ccacttgatc acaatgaatt gcagggtggc gccggcgccc cagaatagcg
     3181 tggttactgc caacgagatt tgcccgagcc ggtcgcgcca aagcagtgcc aggcaatggt
     3241 tgaactcacg taacagatac cagggattct tgtgcagcgc cttgtggtca acaccggtgt
     3301 ccggaatgta caggttgaag atcgacgcca ggatgtagag gccgccgacg atgcaaagcg
     3361 ccatttcgtt ggtggtgtcg attccggtgt cgaaaaatgg aaaattcagc gatagcaggt
     3421 gctttgcgat ggccggctgg atcaaggtgc cgccgatgac tacgcccagg atgattgcgc
     3481 cgacagtcag cccttcaatc cagccgttgg ctaccaccag caggcggtgt ggcaggtatt
     3541 cggtcaggat gccatacttg gccggcgagt aggcggcggc accgagtccg acgacagcgt
     3601 aggcaagcag cggatgaaca gtgagaagca tcaggccgca accgaagatc ttgattgtgt
     3661 tgctgatgaa catgacccgc catttgggca tcgagtcggc aaaagcgccg acaaaggcgg
     3721 ccagggccac gtacgagact gtaaagaaag tctttaacag tggctcgtag gagcttgggg
     3781 aatccatttc gcgcagggcg gcgatggcaa cgatgagcag cgcgttgtcg gcgagcgccg
     3841 aaaagaattg cgccgccatg atcaggtaaa aaccaagggg catggtgaaa acggataccg
     3901 ctcataggga gtgccggatt tataccatga aagcccggaa cttgttagcg cggctgatca
     3961 gcattcattc tggatcggac gttcgtcttg tattgaggca aaatgcggtc gatactctcg
     4021 ttccatcatc gccagagccg aggaatccga tctcatgtcg cgtccgattg aagcccgcat
     4081 cgaccttgcg gcgttgcgtc acaactatct gattgcgaaa agccatgcag ccctccaggg
     4141 ccgacctgcc aaggcgtggg ccgtcgtcaa ggccgatgcc tatggccacg gcctgatgcg
     4201 cgcggccaag gcccttgacg gcgtggcgga cggcttcgcg ctgcttgatc tggccgatgc
     4261 cgtggccttg cgcgattccg gttttcgtca gcccattttg ctgctggagg gcttgttcga
     4321 acccgatgag gtaaccgtat gtgccgaata cggcttgatt ccggttgttc accgcatcga
     4381 acaactgcag atgatccggg ccgccgcctt gccgctgcgt ctgccgatct atctgaaact
     4441 caacaccgga atgaaccggc tcgggttcac gctcgagcag ttgccggcag tcagacgcga
     4501 actggctgtt tccggggcga ttggcgcggt gacgctgatg acgcactttg ccgaagccga
     4561 tggcgggcga gggatcgact ttcagcttga acgcttcgag acaatgaggc aaggctggcc
     4621 cgacacgtct cctgtggaga tttctctggc caattcagcc gccattcttc gttatccgca
     4681 gacggcgcac gactgggtgc gcccaggcat catgctttac ggcggctgtc cctttgccga
     4741 aagcgacgcg gcgagcttca atctcaagcc ggtgatgacc ttgcacagcc gtatcctggc
     4801 cgtccaggag atcaagccgg gcgagcgtgt cggttacggg ggggtgttcg aggccacgcg
     4861 accaacccgg gtgggcatca tcgcctgtgg ctatgcggac ggctacccgc gccatgcacc
     4921 gggtggcacg cccattctgg ttgccggtca gcgcaccgaa acccttgggc gtgtatcgat
     4981 ggacatgctg gcctgcgatc tgaccgagtt gcctgacgcc ggtgtcgatt ccccggtcgt
     5041 gctctggggc gagggcttgc ccgccgacga agtggcggcc gctgccggga cgatcagcta
     5101 cgagctgttc tgtgcgcttg cacgacgcgt gccggtcgtc gaggtctgag gaaccggcta
     5161 tggccaggga aaaaaccatc tacgcgtgca ccgaatgtgg ggccagtgca cccaagtggc
     5221 aaggacagtg cccggattgc ggcgtctgga atacgctggt tgaaacgatt gccgaaagta
     5281 cgccagccaa caagagccga ttcgccgcgc tgagtggtgc gtcccgactg caaacgcttt
     5341 cggagatcgt ggcgaacgaa gaagatcgat tgccgacagg tattggcgag ttcgaccggg
     5401 cgctcggcgg ggggctggtg tccggcggcg tcgtgttgtt gggcggcgat ccggggatcg
     5461 gcaagagcac cttgctcttg caagcactgt ccgaattgtc ggggggccgg catgttttgt
     5521 atgtcagtgg tgaggaatcc ggccaacaga ttgccatgcg tgccaggagg ctggcactgg
     5581 atacgaagca gcttcaactc ctgaccgaga tcaatcttga aaagattctc gccctgttgc
     5641 gactggaaaa accgcaggtg gcggttatcg attcgatcca gacgctgtgg tcggatcagt
     5701 tgagttcggc gcccggctcg gtggcccagg tgcgcgagtg tgcggcgcag ctcacacgac
     5761 tggccaaaca aaccggagtg accgttattc tcgtcggtca cgtgacaaag gatggcgcct
     5821 tggcagggcc gcgcgtgctt gaacatatgg tcgataccgt cctttatttc gagggcgaca
     5881 cgcattccag ctttcgcctg attcgtgccg tgaagaaccg ctatggcgcg gtcaacgaga
     5941 tcggcgtttt cgccatgacc gatcgcggct tgaagggcgt gagcaaccct tcggcaatgt
     6001 ttctgtcgca gcatggacag gaggttcccg gctcctgcgt gctggttacc caagagggga
     6061 cgcgaccgct actggtcgag attcaggcac tggtcgatca gtcgcacggc aacccgagac
     6121 gcctcacggt cggcctcgac gcccagcgcc tggcgatgct gctggctgtt ttgcaccgcc
     6181 acgcgggtat cgtctgtttc gatcaggatg tattcgtcaa tgccgttggc ggggtcaaga
     6241 tcagcgagcc ggccgccgat ctttcagtgc ttctggcgat agtgtcctca ttaaaaaaca
     6301 aagtattacc gcgtaaactt attgtgtttg gtgaggttgg tctggcgggt gagatacggc
     6361 ccgcgccgcg tggccaggag cggctgaagg aggccgtcaa gctcggtttt acgcgggcga
     6421 tgatccctga agctaacaag cccaagcatc cgattgccgg aatcgaggtg atcgccgtgc
     6481 gtcgggttga cgaagcgatg gatcgcttgc gcgaactgga ttgagatcaa aaatgagtac
     6541 ggttgctttt gccctgcgca tgctgcgtcg tgacctgcgg gccggtgagc ttcacttgct
     6601 ggtggcagcg ttggtggttg cagtggcggc cctgactgcg gtcggcttct ttgccgatcg
     6661 cgtgcgtcaa tcactggagc gtgaggccaa tcaactgctc ggcgccgatc tgctgctcac
     6721 gtcggaccat ttatggccgc cgaacgtggc tgcagacgct cgcggacggg gtttggctgt
     6781 cgttgaaact cgaacttttc cgagcatggt tacactgggt gaaggcgaca gcctgcgcgc
     6841 ccatttggct gaaatcaagg cggtttcaga gggttaccct ctgcgcggga gtctgcgaat
     6901 cgcacccggt cgcaatgtgg ccgattctcc ggcaagcggg attccgcaag cgggaacgat
     6961 ctggatcgac gaacggctgg catcgtcgct ttctgccaag gttggggata ccatttcggt
     7021 ggggatgctc gctctgcgcg tggctgccat actgacgctt gagccggatc gaggtctaaa
     7081 tttcttcagc gtggcgccgc ggctgctgct caatatcgcc gatctggagg cgaccggatt
     7141 gatccagcca ggcagccgta tcacctatcg tcttctgctg gccggtgagg cgacattggt
     7201 ggctgaattt cgcggtcgtg tggaggcatc gctgcagcgt ggcgagcgaa ttgaagacgc
     7261 gcagaacggg cgacccgaga tccgcacagc ccttgcacgg gcgcaaacct ttctcggtct
     7321 ttcggctctg ctgacagtgg tactggcggc cgttgccatc gcattggcgt cgcggcgtta
     7381 tatgcagcgg catctggacc cctgtgcggt gatgcgctgt ctgggtgcga cgcaggggtt
     7441 tctgctgggt gtatttctcg gccagttcgc atggctcggg ctgttttccg cggtcctggg
     7501 ttgcgtcatc ggatatcttg cgcatttcgt gctgcatgca tggctcgctc aactgctggc
     7561 gacgccattg ccactccccg gcctgttgcc tgcattacaa ggcagtgtca tcggtgtgtt
     7621 gctgttattc gggttttctg cgccgcccct gattcagctc aagcgcgttt cgacgctccg
     7681 tgtgctgcgg cgtgagtttt cttccggcag cattccgcca tcgcgtttgc ttgcaggtta
     7741 tgcagtggga tttgcggcac tggctggctt gatgttctgg atcgccggag aagtccgtct
     7801 tggcgcttac gtggttggcg ggttttcagt ggcgatgctt ttgtttgcgc tcgtggcgcg
     7861 tgtggtcatt cgttttgccg ccctgctgcg aggcggtgcg cggccaggcg ctcgccaggg
     7921 gggcatcggt tggcgctacg gcctggccag tcttgagcgg catcatgcgg ctagtgtggt
     7981 gcagatcgtc gccctggcat tggctttcat ggcgctgctc ctgctgacgg tcacgcggaa
     8041 cgacatactt gatgcctggc gccaagccct gccaccggaa gcgccgaacc gttttgtcgt
     8101 gaatatacag ccggaccagc ttgacagcgt ccagggtgcc ttcgctgagg accaattgag
     8161 cgccgagttt tcgccgatgg tgcgtggccg tctggccagg gtgaatgatg tggaggtcgg
     8221 tccttcaagc tatgacgatg aacgcgccaa gcgtttgatc gaccgggagt tcaatctctc
     8281 gtaccggatg gatttgccgg aaggaaatga agtgacggcg gggcgctggt tttcagaggc
     8341 cgatgagggg cgtggcgcgg cgtcggtgga agtcggtctg gccgcgacac tcggcctgaa
     8401 gccgggtgat cggcttgagt ttgtcatcgc gggtgagcag gtcacgatgc aggtgatcgg
     8461 cttgcgcaag ctgaattggg attcgatgcg ggtaaatttt ttcgtactga ccccgcccgc
     8521 agtcctgaag ccctacccgg cgagctggat tacaagtttc tacctggcac ccgaaaaggc
     8581 tgatttcgta aatcgtctga ttgccgagtt tcccaatctg acggtggttg acgttgcagc
     8641 gattctgcag cagttgcagt cggtcatgaa tcgggtggcg caggctgttc agttcatctt
     8701 tctgttcacc ctgatggccg gaatcattgt tctttatgcg gcgctggcct cggcggccga
     8761 tgagcggcgt tatgagctgg cggtgatgcg ggcgctgggt gcacggcgcg agcagttgcg
     8821 agctgccttg cttgctgaat tcgcgactat cggtggcttg gctggcctga ttgcatcgct
     8881 gggcgccatt gcagtcgggc agttgctggc agagatggtt ttcaagttcg aggtggcgat
     8941 cgatatttcg cttccgtttg ccgcgatgat ctgcggagcg gggctggtaa ccggcgccgg
     9001 ctggtttgcg gcgtcccgac tgatgaacgt gcctcccttt gaagccttaa gatccggcgg
     9061 ctagcccggg cagttcttct cggcgcagca gaaaaacgaa acgatccccg gcgctggtat
     9121 ccagccaggt gaacaaggtt tccgggaatg ccttttccag atcgtcccgg ttgtgtccga
     9181 tctcgaccac cagcaggcca ttcggattga ggtgtccggc tgcttccttc agcagaattc
     9241 gtacgaaatc aagaccgtcc ttgccaccag ccagcgcgag ctccggctcg cgccggtact
     9301 catccggcag gctatccatc gattctgcgt tgacgtaggg cggattggaa atgatcaggt
     9361 cgtagctttg accctggacc cctgcaaagg cgttggactg gacgaggcgc aggcgtgaac
     9421 cgaggccgta gtcctcgaca ttgcgtttgg ctactgcgag agcgtcgggc gagaggtcaa
     9481 gggcatcgac ctttgattcc gggaaagcgt gggccgcgag gatggccaga cagccggaac
     9541 cggtgcacag atccagaacg cgggtcaccg cccacggatc gtcgatccat ggactgagtt
     9601 gttcgcgcag tagctcggcg atatgcgaac gcgggacaat cacccgttca tcgacgtaga
     9661 aacggtaatc gccaagccag gcttcgttgg tcaggtaggc ggctggaagg cgagcggcga
     9721 tacggcgttt gatcaccttg agcacggcgg cgcgttcgtc gctggtcaag cgggcatcca
     9781 tgaatggatc cagacgatca agcggcaact tcagcgtgtg caacagtaaa taggcggcct
     9841 cgtcccaggc gttatcgttg ccgtgaccaa aaaacagttc ggactcgata aagcgactga
     9901 cagcaaaacg catcaggtcg cgcaaggttg agagttcgtt ttgggcttga tcaaacatat
     9961 cagggcagta gttgttccag aatacggcga taaatggcgg aaagccgggg aagggcagca
    10021 atttcaatgc attcgtcgat cttgtggatg ctcgcattga cggggccgat ttcgatgatc
    10081 tgcgggcaga ggtcggcgat gaaacgacca tccgacgtgc cgccagtcgt tgatagctcg
    10141 gcctcgatgc ccgtttcttc gcggattgcc ttgagtgagg cgtcgaccag cgcaccaccc
    10201 ccggtcagaa agggcttggc accgagtgtc cagtcgattt cgtaatccag gccatggcga
    10261 tcaaggattt ctccggcgcg cgctttcaat tcctccggcg tgctggtcgt ggcaaagcgg
    10321 aaattgaact ggatgagcac gttgccggga atgacgttat tggcgccggt gccaccatga
    10381 atattggaaa tctgccatgt cgtgggcggg aaggacgcgt tgccttgatc ccatatgcaa
    10441 ttgaccagtt cagtcattgc cggtgcgacc aggtggatcg ggtttttcgc cagatgcgga
    10501 taggcgatat ggccttgcac gcccctgaca gtgagcttgc cggtcagcga gccgcgacga
    10561 ccgttcttga tcgtgtcgcc gagacgactg accgccgtcg gttcgccgac aacgcagaag
    10621 tccatcttct cgccgcgcac cgcgagcgct tcggtcacgg caaccgtgcc atccaccgca
    10681 tcgccttcct cgtccgaagt gagcaggaag gcgatggagc cgctatgttc cgggcggcta
    10741 gcgacgaagg cttcggttgc tgtaacaaag gcagcgagcg agcttttcat gtctgccgca
    10801 ccgcggccat agagcaagcc gtctcgccgg gtcggctgga atgggtcgct ctgccactga
    10861 tcaagaggac cggtcggaac aacgtcggta tgcccggcaa agcagaacag gggactggcc
    10921 gagccccggc gtgcccatag gttggtaacg ccaccacggt tgatgtattc gcatccgaat
    10981 cccatacgct ctaggcgctc gccgatgatg cgcatgcagc cgccatcttc gggggtgacg
    11041 gaacggcagg cgatcagttg ctcggcgagc ttgagtgttg cgtcaccggg catctcaggt
    11101 ttcttctggg gcgtcgttga gactcagcga aaattccttg aaggacgctc cagagtcgct
    11161 ggtttccgag aaatccagcg gcgtttcgac gcgtagctct tcgttttgtg tgccgctggt
    11221 gatttcggaa ttgttgatca gccaggaaag gttggtgggc gagtcggcat tccccagcgc
    11281 ttcatcgaga gtgatggtgt tttcacgata gaggcggaac agatcctgtt caaaggtctg
    11341 cgatcccggc gcaagactct gttccattgc ctccttgacg gccaggactt cgccgcgctc
    11401 gatcaattcc tggacgtgac gcgtattgag cagaatttcg gcggcaggcg ttcgctttcc
    11461 gtccggcttc ttgaccaggc gttgcgagac gatggccttc aagctggcgg aaagatcgag
    11521 ataaagcgaa gtgcgggttt ccagcgggaa gaaattgatg acgcggttaa gcgcgtggta
    11581 gctgttgttg gcgtgcagcg tggcgaggca caaatggccg gtctgggcgt aggcgatggc
    11641 ggcctgcatg gtttccttgt cgcggatttc gccgatcagg atgcagtcgg
//
LOCUS       DAORPM010000045        11481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1240_length_11481_cov_12.959391, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000045 DAORPM010000000
VERSION     DAORPM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11481
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1240_length_11481_cov_12.959391"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /gene="lptF"
                     /locus_tag="PLD53_03625"
     CDS             <1..787
                     /gene="lptF"
                     /locus_tag="PLD53_03625"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRC60025.1"
                     /translation="IRPVLTFTLPLVLAIAGLSLFVSPWALSKSAEYRGNLDTRKDAG
                     QASPGAFQESSSGDRVVFVEAVADDESQVKNVFVSSVQNQRQGVTMAAAGHQEIAENG
                     DRFLVLENGRRYEFVPGTPEFRILQYARYAIRLETKEARGIEKTTKNSPTLELMKQDL
                     PAQRAELLWRFSVPLSAIILALLAIPLSFVNPRAGRSANMLLALFTYLLYNNLLTISQ
                     AWVASGKISFAAGMLTVHLFMICLLPLLFYRRIAVLSFSRFSR"
     gene            784..1857
                     /gene="lptG"
                     /locus_tag="PLD53_03630"
     CDS             784..1857
                     /gene="lptG"
                     /locus_tag="PLD53_03630"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRC60026.1"
                     /translation="MRIYRRYLAREVSGAILLVLAGFLALFSFFDMITEVKNVGEGSY
                     QLHHALVFVMLKMPGRIYELMPIAVLIGTLYALSTLARHSEITVLRVSGMSTGRLLSG
                     LFQVAAFFAAITFLIGEVVAPPSERAAQQLRLNQKGRAVGQDLRSGLWVKDERSFINV
                     KVVLPDTRLRGIRIFDFDDSARLRSVTDAQEGSYIPPASWRLSGVVRTDLRDDRAEVT
                     RLPDQEWHSALNPDILAVLMVAPERMSLLHLSAYVRHLAENSQKTMRYDIALWKKVIY
                     PLAALVMVALALPFGYTHNRVSGTSLKIFSGVMIGVFFYMLNGLFSNLGAINSWSPVV
                     SAIAPSALFMLAAGGMIWWVERR"
     gene            complement(1944..2369)
                     /locus_tag="PLD53_03635"
     CDS             complement(1944..2369)
                     /locus_tag="PLD53_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRC60027.1"
                     /translation="MPVTIRSDRPGILRRLAAMLYETLLLFGVLLTTFVFPHVLIGAF
                     AHRLATTTLLWTHLFLVLLVYFIWFWCNGGQTLAMKTWRIRLVSHDGLPIRPAQALLR
                     FLLCWPSLGLAGVGIIWALLDRDRQFLHDRIAGTQLVTA"
     gene            complement(2369..2881)
                     /locus_tag="PLD53_03640"
     CDS             complement(2369..2881)
                     /locus_tag="PLD53_03640"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HRC60028.1"
                     /translation="MTVQQKIILAPLSSDWDSLPFFRQKKWLGIATRFPTMTPEEQRR
                     IQGQMQAWGKLTPEQRQLARENFKTANQLPAEKKQELKLKWQEYSNLPDDIKEKLKRQ
                     AERDSISKPGQPPGQPTPPSPLPPVASKVESPNIAPNLRTPPISPQASGSSAAPPVAV
                     PPTTGTATKP"
     gene            complement(2971..3348)
                     /locus_tag="PLD53_03645"
     CDS             complement(2971..3348)
                     /locus_tag="PLD53_03645"
                     /inference="COORDINATES: protein motif:HMM:NF023699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HRC60029.1"
                     /translation="MNELHFAYKVRQHLNRGLHDLRPETAAHLAESRQSALACQRQVA
                     RQSVLATAGHFAQHYYDSLRIKQVFAAFILLLCLAFAAFWVADQRVTELGDIDSALLA
                     DDLPISAFTDKGFNAWLKRGALE"
     gene            complement(3345..3908)
                     /locus_tag="PLD53_03650"
     CDS             complement(3345..3908)
                     /locus_tag="PLD53_03650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRC60030.1"
                     /translation="MASQRELSDFLAASERRAFKQAMFAVRNEESALDIVQDSMMKLA
                     EKYGDRPAEELPMLFQRIMQNTIRDYYRRSKVRSLWTTLLSALTPNDAEDYDPLETIA
                     VESGSYTPNTPDGQLQQSQVLVIIEKEIQQLPTRQREAFLMRYWEDMDVAETASAMGC
                     SEGSVKTHCSRATHTLATALKAKGITL"
     gene            4050..5744
                     /gene="ilvB"
                     /locus_tag="PLD53_03655"
     CDS             4050..5744
                     /gene="ilvB"
                     /locus_tag="PLD53_03655"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRC60031.1"
                     /translation="MITGAEIVIRCLQEEKVEYVFGYPGGAVLHIYDELFKQDKVKHV
                     LVRHEQAAVHAADGLARSTHKVGVALVTSGPGATNAVTGIVTAYMDSIPLVIISGQVP
                     THYIGQDAFQECDTVGITRPCVKHNFLVKDVNDIAITMKKAFYIANTGRPGPVVVDIP
                     KDITAAKCKFDYPKDIQMRSYTPVVKGHLGQIKKAVKLLLGAKRPMIYTGGGVILSNA
                     APQLTELTRRLGFPITNTLMGLGAYPATDPQFLGMPGMHGTFEANMAMQNCDVLLAIG
                     ARFDDRVIGNPENFALQPHKIIHVDIDPSSISKRVRVDVPIVGNVPDVLEDMLKLIEA
                     ESGKTEIADWWKEIEGWRSRLCMKYTMKDVIMPQYVIEKLYEITGGNAIVTSDVGQHQ
                     MWAAQYYKFDKPRRWLNSGGLGTMGVGLPYAMGAALANPEMQVACVTGEGSIQMCIQE
                     LSTAKQFRLPIKIINLNNRYLGMVRQWQEMFYGSRYSESYMDSLPDFVKLAEAYGHVG
                     IRVERPEDVEGALKKAFIDHKDDLVFLDILTDREANVFPMVAAGKGLSEMILAEDL"
     gene            5757..6248
                     /gene="ilvN"
                     /locus_tag="PLD53_03660"
     CDS             5757..6248
                     /gene="ilvN"
                     /locus_tag="PLD53_03660"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRC60032.1"
                     /translation="MRHIISILLENEAGALSRVAGLFSARGYNIETLTVAPTEDLSLS
                     RLTIVTSGSDEVIEQITKQLNKLIDVIKVVDLSEAAHIERELMLIKVRALGKDREEIK
                     RLADIFRGRIIDVTESTYVIELTGSGSKLDAFIQAIDTDWILETVRTGVSGIGRGDRI
                     LKV"
     gene            6288..7304
                     /gene="ilvC"
                     /locus_tag="PLD53_03665"
     CDS             6288..7304
                     /gene="ilvC"
                     /locus_tag="PLD53_03665"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRC60033.1"
                     /translation="MKVYYDKDADLSLIKGKKVTIVGYGSQGHAHAQNLTDSGVKVTV
                     GLRKGGASWGKVEKAGIKVDEVAKAVKSADVVMMLLPDENIPSVYYNDVEPNIKKGAA
                     LAFAHGFNIHYNQVVPRADLDVIMVAPKGPGHTVRSEYLKGGGVPSLIAVHQNVSKKA
                     KDIALSYAAANGGTKGGVIETSFREETETDLFGEQTVLCGGAVELAKMGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPSVINDQSRQAMRD
                     ALKRIQNGEYAKMFIQEGKTNYPSMTANRRLIAEHPIETVGAKLRDMMPWIKKNKLVD
                     QSRN"
     gene            7417..8052
                     /locus_tag="PLD53_03670"
     CDS             7417..8052
                     /locus_tag="PLD53_03670"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRC60034.1"
                     /translation="MNYPHPILAKEGWPFIAGTLAAAVLTSFSGFWSLPFWLVFIFCV
                     QFFRDPPRVIAGGSKSVLAPADGRIVVVEESDDPYLNRRALKVSVFMNVFNVHSNRSP
                     IRSSVHQKWYKPGSFLNAALDKASLENERCALHLKTTDGRDITCVQIAGLVARRILNY
                     VDAGTDLGAGQRFGFIRFGSRVDLYLPLDSTAQVVIGEKVFASSSVLAELS"
     gene            complement(8126..8551)
                     /locus_tag="PLD53_03675"
     CDS             complement(8126..8551)
                     /locus_tag="PLD53_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HRC60035.1"
                     /translation="MGIVPPERRRNLEDPNPGKAKTTSMTLRSLYLTTCTSLIVLIFL
                     CLAWELRLAPIQPGGTWLVLKCVPLLTPLFGILHGRRYTYQWSSMLILLYFAEGIVRT
                     STDTGMGRWLAAGETLLSVIYFCAAIGYVRLSRNRDDHK"
     gene            complement(8583..10109)
                     /locus_tag="PLD53_03680"
     CDS             complement(8583..10109)
                     /locus_tag="PLD53_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60036.1"
                     /translation="MTDILVDSGSSEADTLSQANMRTLCAWLDAPPLPNASDELTPLL
                     GHLEFLRTSQESPQHCASALGRLYTRSISVCTALLPLLTDVDLPVPLPRKTRQLAHSL
                     QELLQTLAEDLLTTLGRNGNQQNSELSQEQNLTLWRCLNALSKHLLISNLAASPAVNG
                     IWRQLHQIYETVRRQGLEGHVPEEASSSLQNIYFSAILLGCAQPASFTSSEINFIVAC
                     AENSVDLIEPTGDAATRTPATFWIDPSRDATALACSRKLPPPDTHVHYFSCDRLAPKL
                     KNQLDLLENGASPQQINLPDFASTPGGQGVLRRLITYWCEPGKRRFPRRRQNYRAALC
                     SGLHSVWHMFQDGDAAAVETSSWMITNESPDGYALMHVSGKTGGISVGDITAIRTESG
                     ANWQICMVRWALSENQEHLELGLQILATRAVPAHLARPSETGTTVRLPVLILPEIRAL
                     HSTEMLVIPTGTLENQPLKLVLVLEKENLEVREVKSTHLDEQNSHIEIFSIEPDTLPS
                     "
     gene            10311..10565
                     /gene="rpsP"
                     /locus_tag="PLD53_03685"
     CDS             10311..10565
                     /gene="rpsP"
                     /locus_tag="PLD53_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRC60037.1"
                     /translation="MVVIRLARGGAKKRPFYNLVVADSRTRRDGRFIERIGFYNPIAT
                     DKEEGLRIVADRLSYWQKQGARLSLTAARLVKQAGAKVAS"
     gene            10585..11127
                     /gene="rimM"
                     /locus_tag="PLD53_03690"
     CDS             10585..11127
                     /gene="rimM"
                     /locus_tag="PLD53_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288793.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRC60038.1"
                     /translation="MPESVGVVTPADIVVLGKIVGPYGLRGAVKVHPFADDPSHWARL
                     PYWWIGQEGSAPGLWRQTRLLKCNLRGDLLVAELDCLPDRDASEAARGVLVGVPRADL
                     PPAAKGEYYWADLIGLDVINTRGTVLGQVLGLIETPANDVLRVGDGVKVEQLLPFVAT
                     VVLDVDVASRKVCVDWESDW"
     gene            11184..>11481
                     /locus_tag="PLD53_03695"
     CDS             11184..>11481
                     /locus_tag="PLD53_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRC60039.1"
                     /translation="MFVALSHSGVTRRALEEGRWSVNFWNPRDFTEDVHRTVDDRPYG
                     GGPGMVMMPKPLAAAISAAKARQAGAGLVKSRVVYLSPQGAPLTHRRVMQFVQDA"
BASE COUNT         2433 a         2885 c         3433 g         2730 t
ORIGIN      
        1 gatacggccc gtgttaacct tcactttgcc gctggtgttg gcgattgccg ggttgtcgct
       61 ttttgtttct ccctgggcgc tgtccaagag tgccgaatac cgcgggaacc ttgatacgcg
      121 caaggacgcc gggcaggctt ctccaggcgc attccaggaa tcctcatccg gggatcgtgt
      181 ggtatttgtc gaagcggttg ccgatgatga aagtcaggta aagaatgttt ttgtgagttc
      241 ggttcagaac cagcgccagg gagtcacgat ggctgctgcg gggcatcagg aaattgccga
      301 gaacggggat cggttcctgg ttctggagaa cggacgacgt tatgagtttg tacccgggac
      361 acccgagttt cgcattctgc agtatgcgcg ttatgccata cgtctcgaaa ccaaggaggc
      421 gcgtggaatc gagaaaacaa cgaagaattc accaacgctg gaattgatga agcaggatct
      481 gcctgcccag cgggctgagc tgctatggcg ctttagtgtt ccgctatcgg caattattct
      541 ggctctgctg gcgatccccc tttcgttcgt caacccgcgt gcaggccgtt cggcaaatat
      601 gcttttggcg ttgtttactt atctcttgta taacaatttg ctgaccatca gtcaggcgtg
      661 ggtcgctagt ggaaagatct cgttcgcagc gggtatgctg accgtccatc tgttcatgat
      721 ctgcctcctg ccactgctgt tctaccgccg gattgcggtg ctgtcttttt cgcggttttc
      781 cagatgagaa tctaccgtcg ttatctggcc cgagaggtgt ctggcgcgat cttgcttgtg
      841 cttgctggat ttctcgcgtt attcagtttt ttcgacatga tcactgaagt caagaatgtg
      901 ggcgaaggct cttatcagtt gcatcacgcc cttgtgtttg taatgttgaa aatgccgggg
      961 cgcatttatg aacttatgcc gattgctgtc ctgatcggga cgctctacgc tctgtcgact
     1021 ttggccagac attcggaaat taccgtgttg cgggtgtcgg gaatgtcgac cggacgcttg
     1081 ctttcaggat tgtttcaagt ggcggcattt ttcgctgcga tcacttttct gattggtgaa
     1141 gttgtggcgc cgccttccga gcgcgctgcc cagcaattgc gtctcaatca gaaagggagg
     1201 gcagttggcc aggatttgcg cagtggactc tgggtcaagg atgaacgaag tttcatcaac
     1261 gtgaaagtcg tactgccgga cacgcgtttg cgtggtatcc gcatttttga ttttgacgat
     1321 agcgccaggc tgcgttcggt caccgatgcg caagagggat cctatatccc gccggccagt
     1381 tggcggctca gcggcgtcgt tcgaaccgac ttgcgtgatg atcgggccga ggtcacccga
     1441 ttgccggatc aggaatggca ttcagcgctc aatccggata ttctcgctgt gctgatggtt
     1501 gcgcccgagc gaatgtccct gctgcatctg tcagcttacg taaggcatct cgcagagaat
     1561 agccagaaaa caatgcgtta cgatatcgcg ttgtggaaga aggtgattta ccctttggct
     1621 gccctagtca tggtcgcatt ggcgttgcct ttcggctata cccataatcg ggttagcggg
     1681 accagcctga agatcttttc cggggtgatg attggggtgt ttttttacat gttgaatggt
     1741 ctcttctcga acctgggcgc gataaacagt tggtcgccgg tcgtcagcgc catagcgcct
     1801 tcagcattgt tcatgctggc agccggtgga atgatctggt gggttgagcg acgctgattc
     1861 ctggctgatt gccgggaaag cctcattggc acaaagatat tcacctacgg cgccaagcgt
     1921 gagtccggtg gaaacttttc tggttatgcc gtgacgagtt gggtgccggc gatcctgtcg
     1981 tgcagaaatt gcctgtcgcg atcgagcaga gcccagatga tgccgactcc ggcaagtccc
     2041 aggcttggcc agcaaagtag aaagcgcagc agtgcttgcg ccgggcggat tgggagtccg
     2101 tcatgcgaca ccaaccggat tcgccaggtt ttcatggcca gcgtttggcc gccattgcac
     2161 cagaaccaga tgaaatagac gagcaggaca agaaagagat gcgtccacaa tagcgttgtt
     2221 gttgccaggc gatgcgcgaa agcaccgatc agcacatggg ggaagacaaa cgttgtaagg
     2281 agcacgccga acagcaataa cgtttcgtag agcatggccg ccagacggcg cagaattcca
     2341 gggcgatccg agcgaattgt tacaggcatc aaggtttcgt tgctgttccg gtggttggcg
     2401 gtactgcgac cggcggtgct gctgacgatc cggaggcttg cggactgatg ggcggcgtgc
     2461 gcaagttggg ggcaatgttc gggctttcaa ccttgcttgc cacaggaggc agcggtgaag
     2521 gtggagttgg ttggcctggt ggttggcctg gttttgaaat ggaatcgcgt tctgcttgcc
     2581 gcttcagctt ctcctttatg tcatccggga ggttggaata ctcctgccac ttcagcttga
     2641 gctcctgctt tttttctgcc gggagctgat tggcggtttt gaagttctcc cgggcgagct
     2701 gccgctgttc cggtgtcagt tttccccatg cctgcatctg cccctgaatg cggcgctgct
     2761 cttccggcgt catggtagga aaccgagtgg caatacccag ccatttcttt tggcggaaga
     2821 acggcaacga atcccagtcg ctgctcagtg gcgcaagaat aattttctgc tgtaccgtca
     2881 attcgctcca tatcggttga ggtggagttg caacaagcga cgggctggac gtttccgccc
     2941 agcccgaggt cgcaagaaga aaactgagaa ttattccaat gcgccgcgtt ttaaccatgc
     3001 attgaagcct ttatcggtga atgcgctgat cggcaggtca tcggcaagca aggcgctatc
     3061 aatgtcgcca agctctgtga cgcgttgatc ggccacccag aaagccgcaa aggcgaggca
     3121 aagcagcaga atgaacgcgg cgaacacttg cttgatacgc aggctgtcat agtaatgctg
     3181 ggcgaaatgg ccagcggtag caagaaccga ctgcctcgca acctgtcttt gacaagccaa
     3241 ggcgctctgc ctggattcag caagatgcgc cgccgtttcc gggcgtaggt cgtgcaatcc
     3301 gcgattaaga tgctgcctga ccttgtaggc gaaatgcagt tcgttcatag agtaattcct
     3361 tttgctttca aagcagtagc cagcgtgtgc gtggcacgcg agcagtgtgt tttcacactc
     3421 ccttccgagc aacccatcgc ggaagccgtc tccgcaacgt ccatgtcctc ccagtaacgc
     3481 atgaggaagg cttcccgttg acgcgtgggc agctgttgta tctctttctc aatgatcaca
     3541 aggacttgtg attgctgcaa ctgtccgtcc ggagtgttcg gcgtgtacga ccccgattcg
     3601 acggcaatgg tttccagcgg gtcgtaatct tcagcatcat ttggtgtcag cgctgaaagc
     3661 aaagtggtcc acagtgagcg gactttactg cgccggtagt agtcgcgaat cgtgttttgc
     3721 atgatgcgct gaaaaagcat tggcagttct tcggccggcc ggtcgccata cttctcggcg
     3781 agcttcatca tcgagtcttg cacgatatcg agtgccgact cttcgttacg cacggcaaac
     3841 atcgcctgct tgaatgcacg acgttctgaa gcggcaagaa aatctgacag ttcgcgttgg
     3901 ctggccagaa ctgaaatcct tgttgttttc gagtgggatg caatcccttg aatatcgggc
     3961 gcctaggtgg aagccttgac caattatgat ccaggctata acgttacggg ttttcgacca
     4021 tttggccgaa taaggcgagg gtgtttaaca tgataaccgg tgctgagatt gtaatcaggt
     4081 gcctacagga agaaaaggtc gaatatgttt tcggctatcc tggtggtgcg gtccttcaca
     4141 tttatgacga attgttcaag caggacaaag tcaagcatgt tctggtccgc cacgaacagg
     4201 cggcggtgca tgccgcggac ggactggcgc gctcgacgca taaggttggc gttgctttag
     4261 taacctcggg cccgggtgcg accaacgcgg taacgggtat cgtcacggct tatatggatt
     4321 cgatcccgct ggtcatcatc tccgggcagg taccgacgca ctatatcggt caggatgctt
     4381 ttcaggaatg cgacactgtc gggatcaccc gtccctgcgt gaaacacaac ttcctggtca
     4441 aggatgtgaa cgatatcgcc ataacgatga aaaaggcctt ttacatcgcc aacactggcc
     4501 ggcccggtcc ggtcgtggtt gatattccaa aggacattac cgcggcaaaa tgcaagttcg
     4561 actaccctaa agacatccag atgcgctcgt atactccggt tgtcaaaggc catctgggtc
     4621 agatcaagaa ggcggtaaag cttttactcg gtgctaagcg gccgatgatt tacacgggtg
     4681 gaggcgtgat tctctccaat gcagctccac aattgaccga gctaacccgc cgcctcggct
     4741 ttccgattac caacaccttg atggggctgg gcgcctatcc ggcgaccgat ccgcagtttc
     4801 ttggcatgcc cggcatgcat ggcacgttcg aggccaacat ggcgatgcag aactgcgacg
     4861 tactgctggc catcggcgcg cgctttgatg accgggtgat cggcaatccg gaaaactttg
     4921 ccttgcagcc gcacaaaatc attcatgtcg atatcgaccc ttcatcgata tcgaagcgcg
     4981 ttcgcgttga tgtgccgatt gtcggcaacg ttccggatgt ccttgaggac atgctcaaac
     5041 tgatcgaggc cgaatcgggc aagaccgaga ttgccgactg gtggaaggaa attgaaggct
     5101 ggcgttcaag gctgtgcatg aagtacacga tgaaagacgt catcatgccg cagtacgtca
     5161 tcgagaagct ctatgagatt accggtggaa acgcgatcgt cacttcggat gtcggccagc
     5221 atcagatgtg ggcggcgcag tattacaagt ttgacaagcc gcgtcgctgg ctgaactcgg
     5281 gcggtctggg aacgatgggt gtcggcttgc cttacgccat gggcgcggcc ctggcaaacc
     5341 ctgagatgca ggttgcttgc gtcaccggcg aaggctcgat acagatgtgc attcaggagc
     5401 tatcgacagc caagcaattc cgtctgccaa tcaagattat taacctcaat aaccgttacc
     5461 tcggcatggt tcgccagtgg caggagatgt tctacggcag tcgctattcc gagtcgtaca
     5521 tggattcgct cccagatttc gtaaaacttg ccgaagctta cggccacgtt ggcatccgcg
     5581 tcgagcgccc ggaggacgtt gagggcgcgc ttaaaaaagc gttcatcgac cacaaggacg
     5641 atctggtttt tctcgacatc ctcactgacc gggaagcaaa tgtcttcccg atggtggcgg
     5701 cgggcaaagg cctgtccgag atgattctgg ccgaagatct gtaagggaac gacaacatgc
     5761 gacacattat ttcgatactg ctggaaaacg aagccggagc gctttcacga gtggccggcc
     5821 ttttctcggc ccgaggctac aacatcgaga cactgacggt ggcgccaacc gaggatctgt
     5881 cattgtcccg gctgactatc gtaaccagcg gtagcgatga agtaatcgag cagattacca
     5941 agcaattgaa caaactgatc gatgtcatca aagtggtcga tctgtctgaa gcagcgcata
     6001 ttgagcgcga gctgatgctg atcaaggttc gcgccttggg caaggatcgt gaggaaatca
     6061 agcgattggc agatattttt agggggcgca tcatcgatgt taccgagtca acctacgtaa
     6121 ttgaacttac cggtagcgga tcgaagcttg atgcttttat ccaggcaatc gacacggatt
     6181 ggatcctcga aaccgtgcgg actggtgtca gcggtattgg ccgtggcgac cgtattctta
     6241 aggtttgagc ggaagttaat cagtatattt ggtacgaaag gaacgccatg aaagtttact
     6301 acgacaagga cgctgatctc tcgctgatca agggcaagaa ggtcaccatc gtcggttacg
     6361 gctcacaagg ccatgcgcat gctcagaatc tgacggattc tggcgtcaag gttacggttg
     6421 gtctgcgcaa gggcggcgcg tcctggggca aggtcgagaa ggccggaatc aaggttgatg
     6481 aagtggccaa ggccgtcaag agtgcagatg tcgtgatgat gctgttgccc gacgaaaaca
     6541 tcccaagcgt gtactacaat gatgttgaac cgaacataaa gaagggcgcc gcactggcgt
     6601 ttgcccacgg cttcaacatt cactataacc aggtggtgcc gcgtgccgat ctggatgtca
     6661 tcatggtggc gccgaaaggt ccgggccaca ctgtgcgctc cgaatatctc aagggtggcg
     6721 gtgttccttc gctgatcgct gtgcatcaga atgtttccaa gaaggccaag gatatcgcgc
     6781 tttcgtacgc ggcggccaat ggcggcacta aaggcggcgt aatcgaaacc agctttcggg
     6841 aagaaaccga aaccgatctt ttcggcgagc agaccgtgct ttgcggtggt gccgtcgagc
     6901 tggccaagat gggctttgaa acactggtcg aagccgggta cgcaccggaa atggcctact
     6961 tcgagtgtct gcacgagctc aagctgatcg tcgacctgat gtatgagggt ggcatcgcca
     7021 acatgaacta ctcgatctcg aacaatgccg agtacgggga atacgtgacg ggaccttcgg
     7081 tcatcaacga tcagtcgcgc caagccatgc gtgacgcgct caagcgcata cagaacggtg
     7141 aatacgccaa aatgtttatc caggagggga agaccaatta cccttcgatg actgcgaatc
     7201 gccgtctgat cgctgaacat ccgatcgaaa ctgtcggtgc gaagctgcgt gacatgatgc
     7261 cctggatcaa gaagaacaag ctcgtcgacc agtccaggaa ctaatcaatt cagtgaggag
     7321 tgaaggctgt ggggtgaggg gtgaggtgca aggcctcgcc gcctcacccc ttgttattct
     7381 cctctcccct ttctcttaac tcttcacggg agcttaatga actatccgca tccgattctc
     7441 gccaaggagg gctggccgtt catcgccggc actcttgctg cggctgttct gacgtccttt
     7501 tccggtttct ggtcgctgcc gttctggttg gttttcatct tctgcgtgca gttctttcgt
     7561 gacccgccgc gtgtcattgc cggtggatca aagagtgtgc ttgcaccggc tgacggacgc
     7621 atcgtcgtgg tcgaggaaag cgatgatccc tacctaaatc gtcgcgcgct caaggtcagc
     7681 gtgttcatga atgtgttcaa cgtgcattcg aaccgctcgc caatcaggag cagcgttcac
     7741 cagaagtggt acaagccggg cagtttcctt aatgcggcac tggacaaggc ttctttggaa
     7801 aatgagcggt gcgccctgca tctgaagacg acggatgggc gggacattac ctgtgtccag
     7861 attgccggcc tggtcgcacg acgaattcta aattatgttg acgccggcac tgatcttggc
     7921 gcaggccagc gttttggttt tatccggttc gggtcgcgag tcgaccttta tctgccgctc
     7981 gatagcacag cacaagttgt gattggcgag aaagtgtttg catcgagtag cgttttggcc
     8041 gagctttcgt agatttcctc caagtcagcc cagatcggaa atcaaaccaa gcatgagctt
     8101 gtttgatccc gaatggcgcg gcggatcatt tgtgatcgtc ccgattgcgg ctgagtctga
     8161 catagccgat cgccgcgcag aaatagataa ccgaaagcag ggtttccccg gctgccagcc
     8221 agcgccccat gccagtgtcg gtgctggtgc gcacgatgcc ttcggcgaaa taaagcagga
     8281 tcagcatcga tgaccattgg taggtatatc gtcgaccatg caggatgccg aagagcggtg
     8341 tcagcagcgg cacacatttc aaaaccagcc aggttccgcc tggttggatc ggtgcgaggc
     8401 gtaattccca agccaggcaa aggaagataa gcacgatcag actggtgcag gtggtcagat
     8461 agagactacg cagtgtcatc gaggtggtct tggccttgcc ggggtttgga tcctcgagat
     8521 ttcgcctgcg ttcaggtgga acaatcccca cagtaatccc cgcacttgac ggaatccttg
     8581 gtttaggaag ggagcgtgtc aggctcgatg gagaatatct ctatgtggct gttctgttcg
     8641 tcaaggtgag tacttttcac ctcccgcacc tcaaggttct ccttttcgag aacaaggacg
     8701 agtttcaagg gttgattctc caatgtgccg gtagggataa ccagcatttc agtcgaatgc
     8761 aaggctctga tttctggcag gatgagaaca ggcaggcgta cggtggtgcc ggtttcggaa
     8821 ggtcgtgcaa ggtgggcagg caccgcacgt gtcgccagga tttgcaggcc aagttccaga
     8881 tgctcctgat tctctgataa tgcccagcgg accatgcaga tttgccagtt tgctccggat
     8941 tcggtgcgga tcgcggtgat gtctccgacg gatatgccgc ccgtctttcc tgaaacgtgc
     9001 atgagcgcgt aaccgtctgg actttcgttg gttatcatcc agctcgacgt ttcaacggcc
     9061 gccgcatcgc catcctggaa catgtgccac acgctgtgca gaccggaaca cagggcagca
     9121 cggtagttct gtcggcgacg cgggaaacgg cgcttgcccg gttcgcacca gtaggtgata
     9181 agccgacgaa gaacgccttg tccgccaggt gtgctggcaa agtccggaag attgatctgc
     9241 tgcggtgaag cgccattctc gagcaaatcg agttgattct tcagtttcgg cgcgagccgg
     9301 tcgcaggaaa aataatgtac gtgtgtatca ggtggcggca atttgcgaga gcaggctaag
     9361 gctgtagcgt cacgcgaagg gtcaatccag aacgttgcgg gtgttcttgt tgcagcgtca
     9421 ccagtcggtt cgatcaagtc aacggaattc tccgcacatg caacgatgaa gttgatctcg
     9481 ctggatgtga atgacgctgg ctgggcgcat ccaaggagga tcgcggagaa atagatgttc
     9541 tgcagggagc ttgaagcctc ttccgggaca tggccttcga gtccctggcg acggacggtt
     9601 tcatagatct gatgcagttg tcgccagatt ccgttgaccg cgggtgatgc agcaaggttg
     9661 cttatcaaca ggtgcttgga gagcgcgttc aagcacctcc agagggtaag gttctgttct
     9721 tggctcagct cgctattctg ttgattgcca ttcctaccga gagtggtcag caagtcctca
     9781 gccagtgtct ggagcaattc ctgcaagcta tgggccagct gcctggtttt ccgtggaagg
     9841 ggaacgggga gatcaacgtc ggtcaataag ggaagcaggg ctgtgcacac tgaaatgctt
     9901 cgtgtataca ggcgccccag cgctgaggcg caatgctgag gcgattcctg gcttgtgcgc
     9961 aaaaactcca agtgaccgag aaggggtgtc agctcgtcag aggcatttgg cagtggcggt
    10021 gcgtccagcc aggcgcaaag ggtgcgcatg ttcgcctgtg agagggtatc ggcttcgctg
    10081 ctaccggaat ctacaagaat atcagtcata tacgattggt ggagacgggg ttcataatat
    10141 accgtgtgga ttataaacgg cgtcgggaga tttgcgaaac ctcccgacac aacctaggaa
    10201 acgcgagtag atacaggtgt cgcttgtact gctttcaggc aaatatcagc ttgatttttc
    10261 gtgctttatt gttataatcg cacgcttttt cggaatttgg aattaatcct atggtagtta
    10321 ttcggctcgc ccgcggtggt gcgaaaaagc gccccttcta caatttggtg gtggctgatt
    10381 cccgcacccg tcgtgatggt cgcttcattg agcgtatcgg tttctacaat ccgatcgcta
    10441 ctgacaagga agaagggttg cgcatcgtcg ccgatcgcct gagctactgg cagaagcagg
    10501 gtgctcggtt atccttgacg gctgcccgct tggtcaagca ggcaggcgcc aaggttgcaa
    10561 gctgagcgga ctattgacaa tctcttgcct gaatccgtcg gcgtcgtaac gcctgccgat
    10621 attgttgttc tgggcaagat cgtaggccct tatggtttgc gcggtgctgt gaaggtgcat
    10681 ccctttgccg acgatccatc gcactgggcc aggctgcctt attggtggat cggtcaagag
    10741 ggtagcgctc cgggattatg gcgtcagacc cggttgctta agtgcaattt gcgtggcgac
    10801 ttgctggtcg cggaattgga ttgtctaccc gaccgggatg cgtccgaggc cgcgcgtgga
    10861 gttctggtcg gggtgccgcg cgctgatctg ccacctgcag caaagggcga gtattactgg
    10921 gctgatctga tcggtcttga tgtgatcaat acaagaggaa cagtgctggg ccaggtgctt
    10981 ggactgatag agacccccgc gaacgatgta ttgcgggttg gcgatggagt gaaagttgag
    11041 cagttgctgc cgtttgtagc gaccgttgtg ctagacgttg atgtggcgtc aaggaaagtt
    11101 tgtgtcgact gggaaagcga ttggtaattc tggtgccccg taacgagcgc tttgtggccg
    11161 acgtggtgac gctgtttccc gaaatgtttg tggcactttc gcattcaggc gtgacgcggc
    11221 gggcgcttga agaagggcgc tggagtgtga atttctggaa tccgcgcgat tttaccgagg
    11281 acgttcatcg caccgttgat gatcggcctt acggcggtgg cccggggatg gtgatgatgc
    11341 cgaagccgct ggcggccgcg atttcggcgg ccaaggcccg gcaggcaggt gcggggttgg
    11401 tgaagagcag ggtggtctac ctgtcgccgc aaggtgcgcc gctgacgcat cggcgcgtta
    11461 tgcagtttgt gcaggatgcg g
//
LOCUS       DAORPM010000046        11460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1242_length_11460_cov_14.349584, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000046 DAORPM010000000
VERSION     DAORPM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1242_length_11460_cov_14.349584"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2342
                     /locus_tag="PLD53_03700"
     CDS             <1..2342
                     /locus_tag="PLD53_03700"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HRC60040.1"
                     /translation="TRLIKRKDVTGLVNACDAGREGELIFNYIAQHAKSGKPIQRLWL
                     QSMTQQAIKEGFSKLRAGTEMKGLADAAVCRSESDWLVGINGTRAMTAFNSKTGGFHL
                     TTVGRVQTPTLALIVEREQKIRKFKARPYWELEGHFACAAGTYTGKWFDESFAKNKTG
                     QKAEQDEHQRAERLWDETRAKALQSKCAGHPGEVSEETKPSTQLSPLLFDLTSLQREA
                     NSRFGFSAKATLGLAQALYEKHKVLTYPRTDARALPEDYLPTVRATLVMLTGEGVGKG
                     HDEAALARYSPFAHQILARQWVIPNKRIFNNAKISDHFAIIPTLQAPKHLSEPEQKLY
                     DMVVKRFLAVFYPAAEYMISTRITRVEGEPFKTEGKVLVNPGWLAVYGREGQEGDEGN
                     LVAVESKEMVQTDELILNATETRPPPRYSEATLLSAMEGAGKMVDDEELKAAMAGRGL
                     GTPATRAQIIENLLGEQYLHRENRELQPTAKAFSLMTLLNGLGIRELTAPELTGDWEW
                     KLARMERGEMSRDEFMKQIAAMTQHIVDRAKSYDSDTIPGDFGELSTPCPKCGGKIKE
                     TYKKFQCTACDFSLWKIVASRQFEASEIESLLTERKIGPITGFRNKMGRPFGAIIRLN
                     ADNSPEFDFGQGANGDDGNAEEVDFSGQEPLGVCPKCEARVFDRGMSFVCEKSVGPAK
                     SCDFRSGKIILQQPVEEEQMKKLLATGRTDLLKGFISARTKRKFSAFLVRGTDGKVGF
                     EFEKREPKPKSPPKPAKVVSEKPLPKTSKAKSGTSKKSG"
     gene            complement(2434..3348)
                     /locus_tag="PLD53_03705"
     CDS             complement(2434..3348)
                     /locus_tag="PLD53_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRC60041.1"
                     /translation="MTLTELRYIVMLARERHFGRAAEKCHVSQPTLSVALKKVEQRHG
                     VILFERSSSDVRLTPIGEKIARQAEKVLEEADRLTELAVQGKDPLVGSLRLGVIYTIA
                     PYLLPRLIPALHSRAPLMPMYLHENFTVSLAEQLRRGDLDVIIVALPFSEPGIVSYPV
                     YDEAFCVVLPTEHPLAAQSAIPPNQVAAENLLLLGKGNCFRDQVVQACPHLSETGGLE
                     GAIEGSSLETVRHMVASGAGISVAPVSAAKSWAQNEPPLQFRPFTDPAPFRRVVIAWR
                     ATFPRPKAIDALRAAILDAPPAGVSAVP"
     gene            complement(3345..4064)
                     /locus_tag="PLD53_03710"
     CDS             complement(3345..4064)
                     /locus_tag="PLD53_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRC60042.1"
                     /translation="MPKMQLERLLRSQGFGSRPECRALVRSGRVSIDGMLCDDPFAEF
                     EPDGLLFSVDGVLWNYREFAFLALNKPAGFECSHKPKHHPSIYSLFPRVLLTRNIQAV
                     GRLDEDTTGLLLLSDDGQFIHAYTSPKKRIPKVYDVTVKHPVDTLQVDALLEGVQLHD
                     EPARIAATACEVRSEHLLRMTVTEGKYHLVKRMIAAAGNRVEGLNRIAIGGFPLPDSL
                     PIGQWIWLEASDLARLARPQQ"
     gene            complement(4126..4626)
                     /locus_tag="PLD53_03715"
     CDS             complement(4126..4626)
                     /locus_tag="PLD53_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC60043.1"
                     /translation="MEKERAEESPSTHEIAGKGSVPSALDVAAAEEAFSMVLRLVRAV
                     QSGMQSIDATQGMSGSQLLALWQISAQPGLRVAELAEALHIHPSTASNLLDKIEVRGL
                     VRRERSDADNRVVRLFLNDSGIDVVRDLPGPLQGRLRYALREVPLPVLKGLVFGLTSV
                     LDLMGE"
     gene            4781..5704
                     /locus_tag="PLD53_03720"
     CDS             4781..5704
                     /locus_tag="PLD53_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRC60044.1"
                     /translation="MDRSALEQVFSFGSQFSPAMALGFRITLIAALAWFAVILSHRMI
                     RAFRLFISKNLADAEEVKRAATLGRVFRYIASVIISLITVTLILSELGISIAPILGAA
                     GVVGVAVGFGAQSLVKDYFTGFFLLLENQIRQGDVIQAGGKAGVVEEITLRFVRMRDY
                     DGNVHFVPNGTITTVTNMTRGHAHAVVDVGVAYRENVDQALEIMRQTGAELRADPAFA
                     AKILEDVEIVGVEAWADSAVVLRCRFKVAPIEQWGVRREYLRRLKHAFDVAGIEIPFP
                     HLTLYPGQNKDGASPPLHLSIDGAQVPERVK"
     gene            5872..9237
                     /gene="treS"
                     /locus_tag="PLD53_03725"
     CDS             5872..9237
                     /gene="treS"
                     /locus_tag="PLD53_03725"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HRC60045.1"
                     /translation="MHNDDIKPDANSHVDPAWDKDPLWYKDAIIYELHVKAFFDSNGD
                     GIGDFKGLTEKLDYLQDLGVNTLWLLPFYPSPFRDDGYDISDYHNVHPEYGTRADVRN
                     FIREAHRRGLKVITELVINHTSDQHPWFQAARRAPEGSSKRNFYLWSDTDKKFPETRI
                     IFTDSEKSNWAWDEVAGAYYWHRFFSHQPDLNHNNPAVVNAVIRIMRFWLDLGVDGLR
                     LDAIPYLCVREGTANENLHETHDVIKQMRAVIDAHYKNRMLLAEANQWPEDVREYFGE
                     GDECHMAYNFPVMPRIFMAVAQEERHPIIEIMNQTPAIPDNCQWAIFLRNHDELTLEM
                     VTQRERDYMYKMYAADPRMRVNVGIRRRLAPLMENNRDKIELVIFLILTLPGSPVLYY
                     GDEIGMGDNIYLGDRNGVRTPMQWSPDRNAGFSKADPQRLFLPPNMDPIYGYEAKNVE
                     AQTRNTSSLLQWNKRLIAVRKNHKAFGRGSIKFLEPGNRKILAYVREWEDQTLLCVVN
                     LSRNAQPVELDLAAFKTRVPVELLGRSPFPPIGDLPYLLTLAGHGTYCFSLSTNVEVP
                     YWHEERLTRPELPVLVLTQGWLTLSGGKATANTVRRALAATTREQFQNKVMGPYLLSK
                     RWFAAKGHKIERIKILEQDAWETTHGSWLLTIIEVHCAEIPPQLYFLPLAICWDEETS
                     EERRAVLAPWTLAKVRQKERTGILYGAFGAEGFCRSLAEGIGENISLPLGKGRIEFRA
                     TPAFERLADGLAAPVRHPALEQSNTAVYFGNQLFLKGYRRLQFGTNPEVEVGRYLTEH
                     SPFPHVAPVAGSVEFQRADGQAMTLALLQGFTENQGDSWSFAVDYLERFLSEPELASE
                     EATPDPHAYFLSLMELLGRRTGELHQAFGMSTGDTAFEQEPITPEDLSRWSIAVQADA
                     VATLNELESRRTSLPESLHAACDHLAALRPVLLERVSPRALAGISAAKMRYHGDYHLG
                     QVLRVEDDFVIIDFEGEPGRPMEERRQKHSPLRDVAGMLRSFSYAAAVATNRVTAERP
                     ADRSRTGPLVKTWELQSSEAFLAGYRQAIKGCAAWPEEPGVAERLIKFFVIDKALYEM
                     RYEMNNRPDWLAIPLFSLIRSLDRSLEASKEKA"
     gene            9260..10483
                     /locus_tag="PLD53_03730"
     CDS             9260..10483
                     /locus_tag="PLD53_03730"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRC60046.1"
                     /translation="MKVLAMVMAGGQGTRLHPLTAERSKPSVPFGSRYRITDFVLSSL
                     VNSEIHGIYLLVQYKSQSLIEHVNKSWGQTLSFHGQFINVVPPQMREGPEWFQGTSDA
                     VFQNINLIERHAPDMVAVFGADHVYRMDVRQMIDFHHQNSAHVSVAAIPIPLAEASAF
                     GIIDADGAGRIKGFLEKPKTPPPMVSDPSRAYGSMGNYLFDTEVLLTALREAKERGEH
                     DFGHNILPRLIKTHNVYAYNFGDNRVPGIADYEEQAYWRDVGTIDAYYDAHRDAMGEQ
                     PRFKLFNPQWVINSSNYQGPSARILSGHIENTALGPGSLIRGASVRNSILRREVIVEK
                     DVEIDDCIIMDHSVIRRGSRLKRVIVDRYNTIAPNTQIGFDPAADKARYHVSEGGIVV
                     LPKGSEVPDVSCYQQ"
     gene            10511..>11460
                     /locus_tag="PLD53_03735"
     CDS             10511..>11460
                     /locus_tag="PLD53_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC60047.1"
                     /translation="MNLPLTAVWPGAPFPRGATWDGEGVNFALFSENATKVELCLFDP
                     SGKKEVQRIELRERTDLIWHCYLPEARPGLLYGYRVHGPYDPAHGHRFNPNKLLIEPY
                     AKDIVGRLRWSDAHFGYKIGHRQQDMSFDRRDNAAGMPKCRVIDPAFTWGEDKSPHIA
                     WSDMVVYETHVRGFTKNHPDVPPSLRGTYAGLATAPVIEHLKRLGVTSVELLPVHAFL
                     NDRHLLEQGLSNYWGYNSIGFFAADQRYSSSGVVSEFKTMVKTMHSNGIEVILDVVYN
                     HTAEGSHLGPTISFRGIDNASYYRLVGDDRRYCMDYTGCG"
BASE COUNT         2585 a         3314 c         3208 g         2353 t
ORIGIN      
        1 tcacacgact gatcaagcgc aaggacgtga ccgggctggt gaacgcctgt gatgcagggc
       61 gggaaggcga gttgatcttc aactacattg cgcagcacgc caagtccgga aagccgattc
      121 aacgactgtg gctgcagtca atgacgcaac aggcaatcaa ggaaggcttt tcaaaactcc
      181 gcgccggtac cgaaatgaag ggactggccg atgctgcagt gtgccgctcc gaatccgatt
      241 ggttggttgg tatcaacggc acgcgtgcca tgaccgcctt caattcaaag actgggggct
      301 tccatttgac gacggtaggt cgagtacaga ctccgacctt ggcgttgatc gtcgaacgtg
      361 aacaaaagat ccgcaagttc aaggcacgcc cctactggga gttggaagga catttcgcct
      421 gtgccgccgg cacatacacg ggaaagtggt tcgacgagag ttttgccaag aacaagacag
      481 gacaaaaagc ggagcaggat gagcatcagc gtgccgaacg actttgggac gaaacccgag
      541 ccaaggcctt gcaaagcaaa tgcgccggcc atccgggcga agtcagcgaa gaaaccaagc
      601 catcgactca gctttcacca ttgctcttcg atctgaccag cctgcaacgt gaggccaaca
      661 gccgttttgg cttttcggcc aaggccactc tgggcctggc gcaggccctt tacgaaaagc
      721 acaaggtgct gacctatccg cgtaccgatg cacgcgcact tccggaagat tatctgccga
      781 cggtcagggc gacactcgtc atgctcacag gcgagggcgt tggcaaaggg catgatgaag
      841 cggcacttgc gcgctattca ccctttgcac accagattct ggcgcgccag tgggttattc
      901 cgaacaagcg cattttcaat aacgccaaga tatccgatca ctttgccatc attccgacgc
      961 tgcaggcacc gaaacacctc tccgagcccg agcagaaact ttacgacatg gtggtcaagc
     1021 gtttccttgc cgttttctac ccggcggcgg aatacatgat ctccacgcgc atcacacgcg
     1081 tcgaagggga gcccttcaag acggaaggca aggttctggt caatccgggt tggctggctg
     1141 tttatggccg cgaaggacag gaaggcgacg aaggtaatct ggttgccgta gaatcaaagg
     1201 aaatggtgca gaccgacgaa ctcatcctga atgctacaga aacccggccc ccaccccgct
     1261 actccgaggc gaccctgctt tcggccatgg agggggcagg aaagatggtt gacgacgaag
     1321 agctgaaagc cgccatggcc ggccgtggtc tgggcacacc ggctacccgc gcccagatca
     1381 ttgaaaatct gctcggtgag cagtacctgc atcgcgaaaa ccgcgaactc caacccacag
     1441 ccaaagcctt ctcgttgatg acgttgctca atggtctcgg catccgggaa ctcacggcgc
     1501 cggagttgac cggagattgg gaatggaaac tcgcgcgaat ggagcgtggc gaaatgtcgc
     1561 gcgacgaatt catgaagcaa atcgcggcga tgacacagca tattgtcgac cgtgccaaga
     1621 gctacgacag cgataccatc cctggcgatt tcggcgaact ttccacaccc tgtccgaagt
     1681 gcggcggcaa gatcaaggag acctataaga aatttcagtg tacggcatgt gatttttcgc
     1741 tttggaagat cgtcgccagc cgtcagttcg aggccagcga gatcgagtct ttgctgactg
     1801 agcgcaagat cggccccata actggcttcc gaaacaaaat gggtcgtccc tttggcgcca
     1861 tcatcaggct caatgccgac aattcgccag agtttgattt tggccaggga gcaaatggtg
     1921 atgacggcaa cgccgaagag gtcgatttct ccggacagga gccgcttggt gtgtgcccga
     1981 aatgtgaagc gcgtgttttt gatcgaggca tgtctttcgt ttgcgagaaa tcggtagggc
     2041 cggcgaaaag ctgtgatttc cgctccggta agatcattct gcagcaaccg gtggaggagg
     2101 agcagatgaa gaaattgctc gcaactggcc gtactgatct tctcaagggg tttatttctg
     2161 ctagaacaaa gcgcaaattc tcagccttcc tggtacgcgg aacagacggc aaggtcggat
     2221 tcgagttcga gaaacgggaa ccgaagccaa aatcaccgcc taagccagcg aaagtggtga
     2281 gcgagaagcc acttcccaaa acctccaagg caaaatcggg aacaagcaag aagtcaggat
     2341 agaaggattt ccttgagctt cgttaagccg acaggggcag atgtttcacg tgaaacatct
     2401 gcccctgtcg aatcggaatc aaaaacccat taactacgga actgcgctta ctccggccgg
     2461 aggtgcgtcg aggattgcgg ctcgtaaggc gtctattgcc ttcggtcgtg ggaaagtggc
     2521 cctccatgcg ataacaactc gtcggaaggg ggcagggtcg gtaaaggggc gaaactgcag
     2581 tggcggctca ttctgagccc atgactttgc cgctgaaacc ggagcgacgg atataccagc
     2641 cccgcttgcc accatgtggc gtaccgtttc cagcgaactg ccttcaatgg ctccttcgag
     2701 ccctcccgtt tccgagaggt gcggacaggc ttgaacaacc tgatcgcgaa agcagttgcc
     2761 cttacccagc agcaacagat tttcggcagc cacttgattg ggagggatgg ccgattgagc
     2821 cgccaaagga tgttccgttg gcaagacgac gcaaaaagct tcgtcataga cagggtatga
     2881 gacgataccg ggctcggaaa agggtagggc gacaataatc acatccaggt cgccccggcg
     2941 tagctgttcg gccagactga ccgtgaaatt ctcatgcagg tacatcggca tgagcggtgc
     3001 gcgggaatgc agcgccggaa tcagccgcgg caggagatag ggcgcaatgg tataaatgac
     3061 accaagccgt aaggatccga ccaaggggtc cttgccctgt accgcgagct ccgtcagtcg
     3121 gtcggcttcc tcgagcacct tctcggcttg ccgagctatc ttctcgccaa tgggcgtcaa
     3181 gcgaacatca gaagaggagc gctcgaagag gatcacaccg tgtcgctgtt ccaccttctt
     3241 cagtgccaca gacaacgtgg gttggctgac atgacatttc tctgccgcac ggccgaaatg
     3301 ccgttcgcgg gccagcatca cgatgtagcg aagttcggta agcgtcattg ctgaggacga
     3361 gccagacgag ccagatcact cgcttcaagc catatccact gtcctatagg taggctgtct
     3421 ggcaggggga aaccgcctat tgctatccgg ttgagacctt ccactcgatt cccggcggct
     3481 gcgatcatcc gcttgacaag atggtacttg ccctcggtga cggtcattcg gagcaggtgt
     3541 tccgaacgga cttcacaggc ggtagccgcg atacgcgcag gttcgtcatg tagttgaacc
     3601 ccttccagca aagcgtccac ctgaagcgta tccaccgggt gtttgacggt tacgtcgtat
     3661 actttgggaa ttctcttctt tggtgaagtg taggcatgaa tgaactgccc gtcgtctgaa
     3721 agaagcagca agcctgtagt gtcttcgtcc aaacgtccga cggcctgaat atttcttgtc
     3781 agcaatacac ggggaaacag ggaataaata ctcgggtggt gcttcggctt gtgcgagcac
     3841 tcaaaaccgg ccggtttgtt aagtgcaaga aaggcaaatt cccgatagtt ccacagcacc
     3901 ccatcgacag agaacaaaag cccatcgggc tcgaattctg cgaaggggtc atcgcagagc
     3961 atgccgtcaa tgcttactcg tccggaacga accagagcac gacactcagg ccggctgcca
     4021 aatccctgcg atctcagcag tcgttccagc tgcattttcg gcattgtgag gcgctcaatc
     4081 ggggaggaaa catgcagaaa acgcgtgcgg ggcaacgaca atgggctact cgcccataag
     4141 gtccagcacc gaagtcaatc cgaaaaccaa gcccttgaga accggcaagg gcacttcgcg
     4201 caaagcgtag cgtaatcggc cctgcaaggg cccaggaaga tccctgacca catcgatgcc
     4261 cgagtcgttc aggaacagac gcacgacgcg attgtccgcg tcgctccgct cacggcgaac
     4321 aagaccgcgc acctcgatct tgtcgagaag attgctggcg gtagagggat gtatgtgcag
     4381 cgcttcggcc agttcggcaa cgcgtagtcc cggttgcgct gaaatctgcc atagagccaa
     4441 gagctgggag ccgctcattc cctgggtggc gtcgatactc tgcatgcccg actgaaccgc
     4501 gcgtaccagg cgcagtacca tgctgaaagc ttcctctgcc gctgctacat cgagtgctga
     4561 aggaaccgat cccttgccgg caatttcatg agtcgacggt gattcctcag ctcgctcctt
     4621 ttccataacc accgcgactc ctttgtttgc tcgacaaaca tattcgtgca ataatagttt
     4681 ccgcaaacat aagagtcaag cacggtatcg tgtgacgatc ttgaattcac accattcccg
     4741 cagccactca gccgggaatt ggattacgac ggaaattcct atggatagaa gcgcactcga
     4801 acaagtattc tctttcggca gccagttttc gccggccatg gctctgggct tccggatcac
     4861 cctcattgct gccttggcgt ggtttgccgt gatcctttcc cacagaatga ttcgggcttt
     4921 tcgtcttttc atcagcaaga atcttgccga tgctgaggag gtgaagcgtg cggccacctt
     4981 gggcagggtg ttccgctaca tcgcttcggt catcatttcg ctgattaccg tcacgctgat
     5041 cttgagcgaa ctgggcatat ccatcgcgcc tatcctcggt gctgcgggcg tggtgggtgt
     5101 tgccgtgggg tttggagccc aaagcctggt caaggactac ttcaccggct tctttctgct
     5161 actggaaaac cagatacgtc agggggatgt catccaggcc ggtggcaagg ccggggtcgt
     5221 cgaggaaatt acccttcgtt ttgtccgcat gcgcgattac gacgggaacg ttcacttcgt
     5281 ccctaacggc accatcacaa ccgtcaccaa tatgaccagg ggccatgcgc acgcggtagt
     5341 ggatgtcggt gtggcctacc gggagaatgt cgatcaagcc ttggagataa tgcgccaaac
     5401 cggcgccgag ttacgcgccg atcctgcgtt tgccgcaaag atcctcgaag atgtcgaaat
     5461 tgtcggtgtt gaagcgtggg ccgattcggc ggttgtattg cgctgccgtt tcaaagtcgc
     5521 accgatcgag caatggggag tacgccgcga atacctgcgc cgcctcaaac atgccttcga
     5581 cgtcgctgga atcgaaattc cgttcccgca tctcaccctc tatcctggtc agaacaaaga
     5641 cggcgccagt ccacccctgc acctgtcgat tgacggcgct caagtgccag aaagggtaaa
     5701 gtgagcccct ccaattcgag agaacccttc agctacaatt gctgcaatcc tattcaatcg
     5761 caatccaaga attccgcggt ccgccttata cacagcaaca cattcttcca gtgagttgag
     5821 catttgctac agcaagaaca aatatgacta cttgcccggg tggagagaca gatgcataac
     5881 gacgatatca agcctgacgc caactcccac gtagatccag cgtgggataa ggatccgctc
     5941 tggtacaagg atgcaatcat ctacgagctg catgtcaaag ccttttttga tagcaatggc
     6001 gatggtattg gcgacttcaa ggggctgacc gagaagctcg actacctgca ggacctcggc
     6061 gtaaacactc tttggctgtt gccgttctat ccttcgccgt tccgtgacga tggctacgac
     6121 atttccgact accataatgt gcacccggag tatggcaccc gcgccgatgt ccgtaacttc
     6181 atccgcgaag cgcatcggcg cgggctgaag gtgatcaccg aactggtcat caatcacacg
     6241 tccgatcagc atccctggtt tcaggcggcg cgccgcgcgc cggaaggttc gtccaaacgc
     6301 aatttctacc tgtggtcgga tacggacaag aagtttcccg agacgcggat catcttcacc
     6361 gattcggaaa aatccaactg ggcatgggac gaggtagcgg gcgcttacta ctggcaccgt
     6421 ttcttttcgc accagccgga tctgaaccac aacaacccgg cagtagtgaa tgccgtgatt
     6481 cgcatcatgc gcttctggct cgatctggga gtcgacgggc tgcgcctgga tgccatcccc
     6541 tatctttgtg ttcgcgaggg caccgccaac gagaatttgc atgagaccca tgatgtgatc
     6601 aagcaaatgc gcgcagtgat cgatgcccat tacaagaacc gcatgctgtt ggccgaggcc
     6661 aaccaatggc ctgaggatgt acgcgagtac ttcggcgaag gcgatgaatg ccacatggct
     6721 tacaacttcc cggtgatgcc tagaatattc atggcggtcg ctcaggagga gcgtcatccg
     6781 atcatcgaga tcatgaatca gacaccggcg attcccgaca actgtcaatg ggcgatattt
     6841 ctgcgcaacc acgacgaatt gacgctggaa atggtcactc agcgcgagcg cgactacatg
     6901 tacaagatgt acgctgccga tccgcgcatg cgtgtcaatg tcggtatccg tcgccgattg
     6961 gcgccgctga tggagaacaa ccgcgacaag atcgagctgg tgatctttct tatcctgacc
     7021 ctcccgggtt caccggtcct ctactacggt gacgaaatcg gcatgggcga caacatctac
     7081 cttggcgacc gaaacggtgt ccgaacaccc atgcaatgga gccctgaccg caacgcgggc
     7141 ttttccaaag ccgatccaca gcggctcttt cttccgccca acatggaccc gatctacggt
     7201 tacgaagcaa aaaatgtcga agcccagacg cgcaatactt cctcgctact gcaatggaac
     7261 aagcgcctca tcgccgtgcg caagaaccac aaagctttcg gccgtggcag tatcaagttc
     7321 ctcgaaccgg gcaaccgcaa gatcctggcc tacgtgcgcg aatgggagga tcagacgctg
     7381 ctttgtgtgg tcaatctctc gcgcaacgca caaccggtgg aactcgacct tgcggctttc
     7441 aagacccgcg tgccggtcga actgctcggc cgctccccct ttccgccgat tggcgatttg
     7501 ccgtatctgc tgacccttgc ggggcacggt acctactgct tcagcctgtc gaccaacgtc
     7561 gaggtgccct actggcatga agaacgtctg acccggccgg aacttccggt gttggtactc
     7621 acccagggat ggctgacctt gtccggaggc aaggccacgg ccaatactgt gcgccgggcg
     7681 cttgcggcca ctacccggga acagtttcag aacaaggtta tgggccccta tctgttgagc
     7741 aagcgctggt tcgctgccaa gggtcacaag atcgagcgga tcaagatcct tgagcaggac
     7801 gcttgggaga ccactcacgg tagctggctg ctcaccatca tcgaggtaca ctgcgccgaa
     7861 attcctcctc agctctattt cttgccgctg gccatttgct gggacgagga aacaagcgag
     7921 gagcgacgcg cggtcctggc gccctggacg cttgccaagg tgagacagaa ggaacgcacg
     7981 ggcatacttt acggtgcctt cggcgccgaa ggcttttgcc gctccctggc tgagggcatc
     8041 ggcgagaaca tctcgctgcc tttgggcaag ggacgcatcg aatttcgtgc cacgccggct
     8101 ttcgaaaggc tggcggacgg tcttgcggcc cccgttcgcc accccgcgct tgagcagagc
     8161 aatacggcgg tctactttgg aaaccaacta ttcctcaaag gctatcgtcg tctgcagttt
     8221 ggtaccaatc ctgaagtcga agtcggtcgc tatctcaccg aacactcgcc tttcccgcac
     8281 gttgcgcccg ttgccggctc ggtcgaattc cagcgtgctg acggccaagc gatgactctg
     8341 gcgctgttgc agggcttcac ggaaaatcag ggcgacagtt ggagtttcgc tgttgactac
     8401 cttgagcgct tcctctccga gcctgagctt gcgtccgagg aagctacgcc cgatccacat
     8461 gcctatttcc tttctctgat ggaattgctc ggacgccgga ccggcgaact gcatcaggct
     8521 ttcggcatgt ccacagggga cacggccttc gaacaagagc caatcacgcc cgaagatctc
     8581 tccagatggt caatcgccgt gcaggccgat gcggttgcga cactcaacga gctggagagt
     8641 cgccgcacta gcctaccgga aagcctgcac gccgcctgcg accatctcgc agcactgcgg
     8701 ccggtcctgc ttgagcgtgt ttcgccgcgg gcgctggcgg gaatttccgc cgccaagatg
     8761 cgttatcacg gcgactatca tctcggacag gtcctgcgtg tcgaagacga cttcgtaatc
     8821 atcgacttcg agggtgagcc cggtcgtccg atggaggagc ggcgccagaa gcattcgcca
     8881 ctgcgtgacg tggccggcat gttgcgctcc ttcagctatg cggcggcagt ggcaaccaat
     8941 cgcgtcaccg ccgaacgtcc tgccgaccgc tcccgaactg gcccgctggt aaagacctgg
     9001 gagctgcagt cgtctgaagc tttcctggcc ggttaccggc aggcaataaa aggctgtgcg
     9061 gcttggccag aggaaccagg cgtagcggaa cgactgatca agttcttcgt aatcgacaaa
     9121 gcgctctacg aaatgcgcta cgagatgaat aaccgacccg actggctggc gattccactt
     9181 ttcagcttga taagatcact tgatagatca cttgaagcat ccaaagagaa agcatgacgt
     9241 tcaataaagg agaaaactca tgaaagtact cgccatggtt atggccggcg gccagggaac
     9301 acgacttcac cccttgacgg cagaacggtc caagccgtct gtgcctttcg gaagccgtta
     9361 ccgaatcact gatttcgtgc tcagcagtct ggttaattcc gaaatccacg gaatctatct
     9421 gctggtgcaa tacaaatccc agtcactgat tgaacacgtt aacaaatcct ggggacagac
     9481 cctgtccttc cacggccagt tcatcaacgt tgtcccgccg caaatgcgtg aggggccgga
     9541 gtggtttcag ggtacttccg acgccgtgtt tcagaacatc aacctgatcg agcgccatgc
     9601 gccggatatg gtcgccgtct tcggcgccga ccatgtgtac cgaatggacg ttaggcagat
     9661 gattgatttt catcaccaga attccgccca cgtgtcggtg gcggccattc cgattccgct
     9721 ggccgaagcc tcggctttcg gaatcatcga tgccgatggc gccggccgca tcaagggttt
     9781 tctggagaag ccgaaaaccc cgccaccgat ggtcagcgat ccgagccgcg cctacggctc
     9841 gatgggcaac tacctgttcg ataccgaagt gctgctgact gctttgcgcg aagccaagga
     9901 acgtggcgag cacgacttcg ggcacaatat cctgccgcgc ctgatcaaga cccataacgt
     9961 ttatgcctac aatttcggcg ataaccgtgt acccggcatc gccgactatg aagagcaagc
    10021 ttactggcgt gacgtcggca ccatcgacgc ctattacgat gcgcaccgcg atgccatggg
    10081 cgagcagccc cgcttcaagc tcttcaatcc gcagtgggtg atcaattcca gcaactacca
    10141 aggcccttca gcgcgcatcc tctcgggcca tatcgagaac actgccctcg gcccgggttc
    10201 cctgatacga ggagccagcg tacgcaactc gattcttcgt cgcgaggtga tcgtcgaaaa
    10261 ggatgtggaa attgacgact gcatcatcat ggaccactcg gtgatccgcc gcggtagccg
    10321 tctgaagcga gtcatcgtcg atcgctacaa tactattgcg cccaatacac agatcggctt
    10381 tgacccggcg gctgacaaag cgcgatacca cgtcagcgag ggcggcatcg tggtgttgcc
    10441 caaaggttcg gaagtgcccg atgtgtcgtg ctatcagcaa taagtaggaa gcaacgatga
    10501 aggaagaaac atgaatctgc ctttaaccgc cgtttggccc ggcgctcctt ttccgcgtgg
    10561 tgctacttgg gatggcgagg gggtcaactt cgccctcttt tccgagaatg cgaccaaggt
    10621 cgagttgtgc ctctttgatc ccagtgggaa aaaggaagtt cagcgtattg aactgcgtga
    10681 acggaccgac ctgatctggc attgctatct gccggaggcc cgcccgggcc tgctctacgg
    10741 ctatcgcgtg cacggcccct acgatccggc gcatggtcac cgcttcaacc caaacaagtt
    10801 gttgatcgag ccctacgcca aggatatcgt cggccggctc cgctggagcg acgcccactt
    10861 cggctacaag atcggacacc gtcagcagga tatgtccttc gaccgccgcg acaacgccgc
    10921 cgggatgccc aagtgccgcg tcatcgaccc ggcgtttacc tggggcgagg acaagtctcc
    10981 tcacatcgcc tggtccgaca tggtggttta tgaaacgcac gtgcgcggct tcaccaagaa
    11041 ccatccggac gttccgccgt cactgcgtgg tacctacgcc ggtttggcca cagcgccggt
    11101 aattgagcat ctgaagcgtc tgggagtcac ttcggtggaa ttgctgccgg tgcacgcctt
    11161 cctcaacgac cgccatttgc tcgaacaagg tctgtccaac tactggggct acaattccat
    11221 tggctttttt gctgcggatc agcgctatag ctcaagcggc gtggtcagcg agttcaagac
    11281 catggtcaag accatgcact cgaacggtat cgaggtcatc ctcgacgtgg tctacaacca
    11341 caccgccgag ggcagccacc tggggccaac aatctccttc cgggggatcg acaacgcctc
    11401 ctactaccga ctggtcggcg acgatcggcg ctactgcatg gattacaccg gctgcggcaa
//
LOCUS       DAORPM010000047        11396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1257_length_11396_cov_11.971078, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000047 DAORPM010000000
VERSION     DAORPM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_11396_cov_11.971078"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLD53_03740"
     CDS             complement(<1..485)
                     /locus_tag="PLD53_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC60048.1"
                     /translation="MHATLPLLVKTDFPALHRRSVEILQVNLGYRCNQSCLHCHVNAG
                     PQRKEEMTRETIDALIAFIASSPEVKILDLTGGAPELNPHFRHLVVAARARGLKVIDR
                     CNLTILEEPGHEDLADFLAAHRVEIVASLPCYLEENVDKQRGKGTFTASIKGLQRLNA
                     LG"
     gene            complement(585..2030)
                     /locus_tag="PLD53_03745"
     CDS             complement(585..2030)
                     /locus_tag="PLD53_03745"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HRC60049.1"
                     /translation="MSAQISDASPDQVLVDIVDYVLKYDIQSRQAYETACHSLIDALG
                     CAMEALEYPACRKLLGPIVPGTVVPEGARVPGTGYLLDPVQAAFNIGSMVRWLDFNDT
                     WLAAEWGHPSDNLGGILAVADWLSRGAVAAGREPLLMKDVLSAMIRAYEIQGCIALEN
                     SFNQVGLDHVVLVKVASTAVVAGLLGLKRDEMINAVSLAWVDGQPLRTYRHAPNTGTR
                     KSWAAGDATSRAVRLALMAQAGEMGYPSVLTAPVWGFYDVSFKGRPFQFQRPYGSYVM
                     ENVLFKISYPAEFHSQTAVEAAMTIHGQLQSMGRTAEDIRKITIRTHAACLRIIDKKG
                     ALNNPADRDHCIQYVVAVMLLFGRLGAADYEDDVAADPRIDALREKIVCLEDPGFSQD
                     YLDPEKRSIANALSVELNDGSHIDERLVEYPIGHRRRRSEGLPLLEAKFRRNLARRFP
                     ASQQAAILDVSLDHARLQSTPVHEYVDLYVI"
     gene            2253..2531
                     /locus_tag="PLD53_03750"
     CDS             2253..2531
                     /locus_tag="PLD53_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC60050.1"
                     /translation="MIASILKHLRANGEQLDSEISQALNIPMAQLKTHVAQLSASGDV
                     ICCKVTRFIEGKKIEGISCRLSCDLPAPARGRKPGVKKDPNADNKYPE"
     gene            complement(2589..4076)
                     /locus_tag="PLD53_03755"
     CDS             complement(2589..4076)
                     /locus_tag="PLD53_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HRC60051.1"
                     /translation="MNAVQNLQENTQPLSFIPASEFKNRTTEAVSDQHLRQSFRGAMD
                     FLMAKRAGHFPDLAAFEAQRALAEHVRKYSLSKLPELLEQLERTLTANGVKVHWAENA
                     QQANAIIVGIAQKHAAKLMVKGKSMVSEETELNHAMEAAGIEALESDMGEYIVQLDGE
                     KPSHIIMPAIHKTKVQIAKLFAEKVPGAKYTESVDELIGIGRAVLREKFMTAEIGLSG
                     VNFAVAETGTLCLVENEGNGRMCTSVPNVHIAITGIEKVVEKLEHVPPLYGLLTRSAT
                     GQAITTYFNMISRPRQPGDKNGIGAELDGPKEVHLVLLDNGRTQAYADEQLRATLQCI
                     RCGACMNHCPVYTRIGGHAYGTTYPGPIGEIISPHMMGLAATHALPTASTLCGACEEV
                     CPVKIPIPDLLIRLRGEATHEARPGHAPLVGQGAAKSTLMNVVWAGWAALYTRPVLYR
                     AFGWLATRFRFLTPPTQSGWTVSRTPLTPAARTMHELLAQRRRGK"
     gene            complement(4076..4759)
                     /locus_tag="PLD53_03760"
     CDS             complement(4076..4759)
                     /locus_tag="PLD53_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRC60052.1"
                     /translation="MSARDNILRRLRSAPKGKPILPPDINAFYAAVRSNSPQENRTQR
                     IERFCEKMAFWRGEVVRVTHANWAKTLQGLCAAKNVNSLLYGSHSPHAAALEAAGING
                     LKPYDRPVDEWKTELFRETDAGFTSTRGGIAETGTLIVWPDAREPRLMSLVPPIHFAL
                     LDSDQLHDTLFDAMTTQGWSDPSSPMPSNALLISGPSKTADIQITLAYGAHGPKELVV
                     LLLVNEEEL"
     gene            complement(4756..5532)
                     /locus_tag="PLD53_03765"
     CDS             complement(4756..5532)
                     /locus_tag="PLD53_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRC60053.1"
                     /translation="MERGNRYSQRPTDVYFYATCLVDLFCPEAGMDAITLLEREGITV
                     HFPEDQTCCGQPAYTSGFANEARAVALTQMRLFSEDWPVVIPSGSCAGIMRHHYPKLF
                     ADEPQLKAKAEALAERVFELSEFLLNVLKVQWKDAGKPTTVTLHTSCAARREMGTHEH
                     ARALLAQLPNVNVALQHHESECCGFGGTFSLKHPSISSAMAGDKADALKETGAEAFIS
                     ADCGCMMNINTTLQKRGDAFQGRHLASFLCERLAPKGEKE"
     gene            5689..6465
                     /locus_tag="PLD53_03770"
     CDS             5689..6465
                     /locus_tag="PLD53_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRC60054.1"
                     /translation="MSKPKTQFQVPRISDVVASSLERRILEGSLKPGDRLPPERELAI
                     ELGVSRPSLREAIQKLVSKGMVQSRQGGGTFVTDRLESTFFDPWHEMMGTHPTLREDL
                     LEFRRMLEGQAAEWAAERATEADLVRLKQAFTAMNAAFDSDDLARSSSADIAFHQAIG
                     EASHNVLIGQITAALLRLMHDHIHVNLGELKNIPTAISLLKNQHTAIFTAICEAKPVV
                     ARAAAETHIDYVRESLTESLRSAVRRETAARRLNAEFAEI"
     gene            complement(6487..6933)
                     /gene="smpB"
                     /locus_tag="PLD53_03775"
     CDS             complement(6487..6933)
                     /gene="smpB"
                     /locus_tag="PLD53_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRC60055.1"
                     /translation="MSIIANKKAFHDYFVEERFEAGIVLQGWEVKAIRAGRANIKESY
                     VVIRSGEVFLFGMHITALPEASTHVHPDPIRTRKLLLHENQINKLIGQVERAGYTLVP
                     LNLHFTRGRIKLEIGLAKGKKQHDKRESEKERDWDREKARLMRVKA"
     gene            7010..7453
                     /locus_tag="PLD53_03780"
     CDS             7010..7453
                     /locus_tag="PLD53_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HRC60056.1"
                     /translation="MAMVEKSVLIERSAQQMFDLVDDVEGYPGFLPWCSRTRVEFRDE
                     KKTVATLHVNYRSVKTHFTTENEKEIPLSMSIKLVDGPFRRLEGIWYFKPLTDNACKI
                     EFRLSYEFSSKVFEKVIGPVFSQIANTFVDAFVMRANQFYGVSNA"
     gene            7446..7757
                     /locus_tag="PLD53_03785"
     CDS             7446..7757
                     /locus_tag="PLD53_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HRC60057.1"
                     /translation="MPEPIRVEVVYPLPQEQEIFSIQMPAGATVRQAVEACGVLQKYP
                     EIDLANNKLGVFAKLVKPDAPLRDRDRVEIYRPLIADPKEVRKQRAADGKAMKKGAAE
                     L"
     gene            complement(7784..8233)
                     /locus_tag="PLD53_03790"
     CDS             complement(7784..8233)
                     /locus_tag="PLD53_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRC60058.1"
                     /translation="MKRFVFTLAVTALFAAAGVNAQVYQWKDENGKTIYSDKPPIGAP
                     GQPQKIHAPSPAASSATQKTLADRDLEFRKRQKESQESAEKAQKEQSASAEKQQYCDN
                     TRRRLQALESGERISMHDDKGERYYMDDAQREKEITKARQDIGSNCR"
     gene            8296..9756
                     /gene="guaB"
                     /locus_tag="PLD53_03795"
     CDS             8296..9756
                     /gene="guaB"
                     /locus_tag="PLD53_03795"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRC60059.1"
                     /translation="MRLIQRALTFDDVLLVPAHSEVLPRDVSLRTRLTRNISLNMPLV
                     SAAMDTVTEARLAIALAQEGGIGIVHKNLSPKAQASEVAKVKRFESGILKDPITVPPT
                     MSVREVFELTVLHKISGFPVLEGKVVVGIVTNRDLRFETNLDQPVRNIMTPRDRLVTV
                     KEGATVEEGKALIHKHRLERVLVVNDAFELRGLITVKDIIKTTEHPLASKDGSGRLRV
                     GAAIGVGAGTAERAELLAEAGVDVIVVDTAHGHSQGVLERVQWVKRNLPQVEVVGGNI
                     ATADAARALIDHGADGVKVGIGPGSICTTRIVAGVGVPQITAIQMVSDALTNTGVPMI
                     ADGGIRYSGDICKAIAAGANAVMLGGLFAGTEEAPGDVELYQGRSYKSYRGMGSIGAM
                     AAGAADRYFQDSGSNIDKLVPEGIEGRVPYKGSVLAVIHQLTGGLRSSMGYLGCATIE
                     ELHERAECVEITASGVRESHVHDVQITKEAPNYHVD"
     gene            9831..11396
                     /gene="guaA"
                     /locus_tag="PLD53_03800"
     CDS             9831..11396
                     /gene="guaA"
                     /locus_tag="PLD53_03800"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRC60060.1"
                     /translation="MTHQKILILDFGSQVSQLIARRVREQQVYCELHPFDVSEDFVRQ
                     FNPQGIILSGGPNSVYESEEWRAPQVVFDLGIPVLGICYGMQTMAQQLGGKVESSGKR
                     EFGYAEVRARGHSELLKGLQDRSNDAGHGLLDVWMSHGDKVTELPPGFKVIASNSTCP
                     IAAMADESRKFYAVLFHPEVTNTLKGKQIFSRFVHDICGCRNDWTMPGYIDEAVARIR
                     AQVGQDEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDNGLLRLDEAEQVMDTFARN
                     LGVRVIHVNATEQFMSHLKGVSDPEQKRKIIGREFVEVFQVESQKRANAKWLAQGTIY
                     PDVIESAGAKTRKAHTIKSHHNVGGLPETLNLKLLEPLRELFKDEVRELGVALGLAHE
                     MVYRHPFPGPGLGVRILGEVKPEFADLLRRADAIFIDELRAAGWYEKTSQAFAVFLPV
                     KSVGVMGDGRTYEYVVALRAVETQDFMTAYWAELPHSLLGKVSNRIINEVRGINRVVY
                     DISGKPPATIEWE"
BASE COUNT         2258 a         3152 c         3467 g         2519 t
ORIGIN      
        1 ccgagcgcgt tcaggcgctg caagcccttg atgctggcgg tgaacgtgcc cttgccgcgt
       61 tgcttgtcga cgttctcttc gagatagcag ggcaatgagg cgacgatctc aacgcgatgc
      121 gccgccagaa aatcggccag gtcctcatga cccggctctt ccaggatggt caggttgcag
      181 cggtcgatga ccttgaggcc acgtgcgcgg gcggcgacta ccagatggcg gaagtgcggg
      241 ttcagttccg gcgcaccacc ggtcagatcg aggatcttga cttcggggct ggacgcgata
      301 aaggcgatca gcgcgtcgat ggtttcccgg gtcatctctt ccttgcgctg cgggcccgca
      361 ttgacatgac aatgcagaca gctctggttg cagcggtagc cgaggttaac ctgcagaatt
      421 tcgacggatc gccgatgcag tgccgggaag tctgtcttta cgaggagggg cagggtggcg
      481 tgcattgttg ttgtctttca ggttgtttgc acaagcttgg tcggattcga agggcatttc
      541 cttacagcct aaatttgccg cgcgatctgg ctcccaattc ggcctcagat tacgtagagg
      601 tccacgtatt catggaccgg cgtggactgt aaccgggcat gatccagcga tacgtcaagg
      661 atggccgctt gctgactggc tggaaagcga cgggcgagat tacggcggaa tttggcttcg
      721 agcagcggaa ggccttcgct gcggcgtcgc cggtggccga tcgggtactc gaccaggcgt
      781 tcgtcgatgt ggctgccatc gttgagttcc acggacagcg cattggcaat cgagcgtttt
      841 tccgggtcca ggtaatcctg gctgaaccct gggtcttcaa ggcaaacgat cttttcgcgc
      901 agggcatcga tgcgtgggtc ggcagcgaca tcgtcttcgt agtctgccgc gccaaggcgg
      961 ccgaaaagaa gcatcacggc gaccacatac tggatgcagt ggtcgcgatc tgccgggtta
     1021 ttgagcgccc ctttcttgtc gatgatgcgc aggcaagcgg cgtgggtgcg gatggtgatc
     1081 ttcctgatgt cttcagctgt cctgcccatg gactgcagtt gtccatgaat ggtcatggcg
     1141 gcttccacag cggtctggct atggaattcc gccggatagc tgatcttgaa cagcacattt
     1201 tccatcacat acgagccata gggtcgctga aactggaagg gccggccttt gaacgacacg
     1261 tcatagaatc cccagaccgg cgctgtgagg acgctcgggt agcccatttc cccggcttga
     1321 gccatcagcg ccaggcgcac cgcccggctg gtggcgtcgc cggccgccca gcttttgcgc
     1381 gtgccggtgt tgggggcgtg gcggtaggtg cgcagaggct ggccgtcgac ccaggccaag
     1441 gacacggcgt tgatcatttc gtcgcgcttg agccccagaa gaccggcgac cacggcggtc
     1501 gacgccactt tgaccagtac gacgtgatca agcccgacct ggttgaagga attttccaaa
     1561 gcgatgcaac cctggatctc gtaggccctg atcatcgcgc tgagcacgtc cttcatcagc
     1621 agcggctcgc ggcctgcggc gacggcgccg cgcgacagcc agtcggccac cgccaggatg
     1681 ccacccaggt tgtcggaagg atgtccccac tctgccgcca gccaggtgtc attgaagtcc
     1741 agccagcgga ccatggagcc aatattgaag gctgcctgca ccgggtcgag caggtagcca
     1801 gttccgggca ccctggctcc ctcgggaact acggtgcccg gaacgatcgg cccgagaagt
     1861 ttgcggcagg ccggatactc cagggcttcc atggcgcaac ccagggcgtc gatcaggctg
     1921 tggcaggctg tctcgtaagc ctgccgtgac tggatatcgt atttcagaac gtaatcgacg
     1981 atatcgacca gaacctgatc gggggatgca tcactgattt gtgcagacat gaaagatttt
     2041 cgtcaaagcc ctgcggcagg ggtacggtta cctgtagaat gcgcgccttc tttacgcagc
     2101 acccgatacg gaccctgtgg tccattttga ccagtacgcg ccatttcacc ctgatatacc
     2161 gggtggggtc aatcccggtc gacaattgcc atcgtggcaa ttagggcgca aactagacgt
     2221 ctatttacag aaagcagtgt ggggataccc agatgatcgc atccattctt aaacatctga
     2281 gagccaacgg cgagcagctt gatagcgaga tttcgcaagc gctcaatatc ccgatggcac
     2341 aactgaaaac ccatgtcgcg caactctccg cttcgggcga tgtgatttgc tgcaaggtaa
     2401 cgcgtttcat cgagggcaag aaaatcgaag gcatcagttg ccgcttgtca tgcgacttgc
     2461 cggctccggc gcgcgggcgc aaacccggtg tgaaaaaaga tcccaacgct gacaacaagt
     2521 atcctgagta gacgaggatg ccgttgcagg gctgtttcag gttcctgtca aacctctcct
     2581 tacgcttttc actttccgcg tctgcgctgc gctaacagct cgtgcatggt gcgtgcggca
     2641 ggcgtcagcg gcgtgcggct gacggtccaa cctgattgcg tcggtggggt caggaaacgg
     2701 aagcgcgtcg ccagccagcc gaaagcgcga taaagcaccg ggcgcgtata gagcgccgcc
     2761 cagccggccc agacgacatt catcagcgtg ctcttcgccg cgccctggcc aaccagcggg
     2821 gcatggccgg gacgtgcctc gtgcgtcgct tcgccgcgca ggcggatgag caggtcgggg
     2881 atcgggattt tcaccgggca gacttcctcg caggcgccgc atagcgtcga ggcggttggc
     2941 agggcgtgcg ttgcggccag gcccatcatg tgcggcgaga tgatttcgcc gatcgggccg
     3001 gggtaggtgg tgccgtaggc gtgaccgcca atgcgggtgt aaaccgggca atggttcatg
     3061 cacgcgccgc agcggatgca ctgcagggtg gcgcgcaact gttcgtcggc ataggcctgg
     3121 gtgcggccat tgtcgagcag caccaggtgg acttccttgg ggccgtcgag ttcggcgccg
     3181 atgccgttct tgtcgcccgg ctggcgcggc cggctgatca tgttgaagta ggtggtgatc
     3241 gcctggccgg tcgccgaacg tgtcagcagg ccgtaaagcg gtggcacatg ctccagcttc
     3301 tcgacgacct tttcaatacc ggttatggcg atatgaacgt tcggcacgga ggtgcacatg
     3361 cggccgttgc cttcgttctc gaccagacac agcgtgccgg tttcggcaac ggcaaagttg
     3421 acgccggaca ggccgatttc ggcggtcatg aatttttcgc gcagcacggc gcggccgatg
     3481 ccgatcagtt cgtcgaccga ttccgtgtat ttggcgcccg gcactttttc ggcgaacagt
     3541 ttggcgatct gcaccttggt cttgtgaatc gccggcatga tgatgtgcga cggcttctcg
     3601 ccgtcaagct ggacaatgta ttcgcccata tcgctctcca gcgcttcgat accagccgct
     3661 tccatcgcgt ggttgagctc ggtttcttcg ctgaccatcg acttgccctt gaccatcagt
     3721 ttggcggcgt gcttctgggc gataccgacg atgatggcgt tggcctgttg cgcattttcg
     3781 gcccagtgca ccttgacgcc gttggcggtg agtgtgcgtt caagctgttc gagcagctcg
     3841 ggcagttttg acaggctgta tttgcggacg tgctcggcga gcgcgcgctg cgcttcaaac
     3901 gctgcaaggt ccggaaagtg ccccgctcgc ttggccatca gaaaatccat ggcgccgcgg
     3961 aaactctggc gcagatgctg atcggatacg gcttcggtag tgcgattctt gaattcgctg
     4021 gccgggatga aactgagtgg ctgggtgttc tcctgaagat tctgcactgc gttcatcaca
     4081 gctcctcctc attgaccagc agcaagacga ccagttcttt cggtccgtga gccccatatg
     4141 ccagcgtgat ctgaatgtcg gccgttttcg atgggccgga aatcagcaag gcattactcg
     4201 gcatcgggct ggacgggtcg ctccagccct gagtggtcat cgcatcgaac agcgtatcgt
     4261 gaagctggtc cgagtccagg agggcaaaat ggattggtgg cacgagggac atcaggcgcg
     4321 gttctcgtgc gtcaggccaa acgatcaggg tgccggtttc agcgatgccg ccgcgcgtcg
     4381 aggtgaaccc ggcatcggtt tcgcgaaata gctcggtctt ccactcatcg accgggcggt
     4441 cgtatggctt caagccattg atgccagcgg cttcaagtgc tgcggcatgc ggcgagtgcg
     4501 agccgtagag cagcgagttg acgttcttgg ccgcacacag tccctgcaag gtcttggccc
     4561 agttggcgtg cgtaacgcgg acgacttcgc cgcgccagaa agccattttc tcgcaaaaac
     4621 gctcgattcg ctgcgtacgg ttctcctgtg gcgaattgct gcggacagcg gcgtagaaag
     4681 cattaatgtc gggaggcagt attggtttgc ctttcggcgc ggaacgcaaa cgccggagga
     4741 tgttgtcccg ggcgctcatt ctttttctcc cttcggagcc aggcgctcac acagaaaact
     4801 ggccagatgt cggccctgga aggcatcccc acgcttctgc agggtcgtgt tgatgttcat
     4861 catgcagccg caatcggccg aaataaaagc ttcggcaccg gtttccttga gcgcgtctgc
     4921 cttgtcgccg gccattgccg atgaaatcga cgggtgcttg agcgagaagg ttccgccaaa
     4981 accgcagcat tcggattcat ggtgctgcaa ggcaacgttg acattgggca gttgtgcgag
     5041 cagggcgcgt gcgtgttcat gcgttcccat ctcccggcgt gcagcgcagg aggtatgtaa
     5101 cgtcaccgtc gttggcttgc cagcatcctt ccactgtacc ttgagtacat tgagcaggaa
     5161 ttcggaaagt tcgaaaaccc gctcggccag tgcctcggct ttggctttca gttggggttc
     5221 gtcggcaaag agcttggggt aatgatgacg catgatgccg gcacaggagc cggaaggaat
     5281 gaccacaggc caatcttctg aaaagagccg catctgcgtc agcgccaccg cgcgtgcttc
     5341 gttggcaaag ccgctggtgt aggcgggttg tccgcagcag gtctgatctt ccgggaaatg
     5401 cacagtgatg ccttcgcgct caagcaaggt gatggcatcc attccggcct cagggcaaaa
     5461 aaggtcgacg agacaggtgg catagaaata gacatctgtc gggcgttgac tgtatcgatt
     5521 gcctctctcc atggcggctc ctgtggttaa ttggtaagac caaaagaatc attgtggaat
     5581 gtaaggcact taggctggtt agtcaactct gcatggcaaa ccatttcggc ttgcgatttg
     5641 cttcgtgtcg tcatagaatc atgtggtctt accaatcgag tgacagcttt gtctaagcct
     5701 aaaacacagt ttcaggtgcc gcgcatctcc gatgtcgttg cgtcctcctt ggagcgacgc
     5761 atccttgagg gttcgttaaa gccgggtgac cgtctgccgc ctgaacgcga gttggccatt
     5821 gaattgggcg tgtcgcgccc gtcgttgcgc gaagcgattc agaagctggt atccaaaggc
     5881 atggtgcaaa gccggcaggg cggcggcact ttcgtgactg atcggcttga gtcgactttc
     5941 ttcgatccgt ggcatgaaat gatggggacg catccgacgc tgcgtgaaga cctgctcgaa
     6001 ttccgccgca tgcttgaagg gcaggccgcc gaatgggctg ccgagcgggc gaccgaagcc
     6061 gatctggtcc gcctgaaaca ggcttttact gcgatgaatg ccgcttttga ttccgatgat
     6121 cttgcccgta gttccagtgc cgacattgct ttccatcagg ccatcgggga agccagtcac
     6181 aacgtactga ttggtcaaat cacggctgcg cttctgcgcc tgatgcacga ccatatccat
     6241 gtcaatctcg gtgaactgaa gaatatcccg accgcaattt ctctcctgaa gaaccagcac
     6301 accgcaatat ttaccgcgat ttgcgaagcc aaaccggtag tcgctcgtgc ggcggcagaa
     6361 acgcatatcg attacgttcg cgaatcgctg actgagtctt tgcgttctgc cgtcaggcgg
     6421 gaaacggcag cccggcgttt gaacgccgaa tttgccgaaa tatgagcgcg ttgatcgtca
     6481 gggttttcat gccttgacgc gcatcaggcg cgccttttct cgatcccagt cgcgctcctt
     6541 ttccgactcg cgcttgtcgt gctgcttctt gcctttggcc aggccgatct caagcttgat
     6601 tcgaccgcgg gtaaagtgca gattcaatgg cacaagcgta taaccggcgc gttcgacctg
     6661 gccgatcagc ttgttgatct ggttttcgtg aagcagcaat ttgcgtgtgc ggatcgggtc
     6721 cggatgcaca tgggtcgagg cttcgggcaa agctgtgatg tgcatcccga agaggaaaac
     6781 ctcgccgcta cggatgacga cataggactc cttgatgttc gcgcgtcccg cccgaattgc
     6841 cttgacctcc caaccctgga gcacaatacc ggcctcgaat ctctcttcga cgaagtagtc
     6901 gtggaaggct ttcttgttgg cgatgatgct catgatgcgg ctctcagtgg ttatcgagcg
     6961 agaatgctag aatgcaggat tctacaggat tcgcaaggcg cggctaacga tggccatggt
     7021 tgagaaatcg gtattgatcg agcgttcggc gcaacagatg ttcgatctgg tcgacgatgt
     7081 tgagggttac cccgggtttt tgccctggtg cagccggaca cgggtcgagt tccgggacga
     7141 gaaaaagacg gtggctacac tgcacgtcaa ttatcggagc gtcaagacgc acttcaccac
     7201 tgagaacgag aaggaaattc cgctgagcat gagcatcaaa ctggttgatg gcccgtttcg
     7261 caggcttgaa gggatatggt atttcaaacc gttgaccgac aatgcctgca aaatcgaatt
     7321 caggctttct tacgagtttt ccagcaaggt ttttgaaaag gtcatcggcc cggttttcag
     7381 ccagatcgcg aacaccttcg ttgacgcatt tgtcatgcga gccaaccagt tctatggggt
     7441 ttcgaatgcc tgaaccgatt cgcgtcgaag tggtttaccc cttgccgcag gaacaggaga
     7501 tcttttccat ccagatgccc gcaggggcga ccgtgcgcca agccgttgaa gcttgcggcg
     7561 tgctgcagaa atatcccgag atcgatcttg ccaataacaa gcttggtgtc ttcgccaagc
     7621 ttgtcaagcc ggatgcgcca ttgcgcgatc gggatcgcgt ggaaatctac cggccgttga
     7681 ttgccgatcc gaaggaagtg cgcaagcaga gggcggcaga cggcaaggcc atgaaaaaag
     7741 gtgcggcaga actataaggc gtgcttcacc aagacgcgta cctttacctg caattcgagc
     7801 cgatgtcttg gcgcgccttc gtaatttcct tttcacgttg cgcatcatcc atgtaataac
     7861 gctcaccctt gtcgtcatgc atggagattc gttcgccgga ttcaagtgcc tgcaagcgac
     7921 gtcgcgtgtt atcacagtat tgctgctttt cggctgaagc ggattgctcc ttctgcgctt
     7981 tctcagcact ttcctgggat tctttctggc gcttgcggaa ttccaggtct cgatcagcca
     8041 gcgtcttctg cgttgcagag cttgcggcgg gggagggcgc atgaatcttc tgcggctggc
     8101 caggagctcc gattgggggc ttgtccgaat agatcgtctt tccgttctca tccttccact
     8161 ggtaaacctg agcgtttacg ccggctgcgg cgaagagagc ggttacggcg agagtaaaga
     8221 caaatcgttt catggtcatc cggtcctagc aaggcatacg gtataatact tatttgtgac
     8281 ccaggatcat aggccatgcg tttaattcag agggcattaa cgtttgatga tgtccttctc
     8341 gtgccagcac actccgaggt cctgcctcgc gatgtcagtc tccgaacccg cctgacgcgc
     8401 aacatttctc tcaacatgcc actcgtgtcc gcggccatgg acaccgtcac cgaagcgcgt
     8461 cttgccatcg ctctggctca ggagggtggt attggtatcg tacacaagaa tctcagcccg
     8521 aaggcccagg cgtctgaagt ggcgaaggtc aagcgtttcg agtcgggcat tttgaaagat
     8581 ccgattactg tcccacccac catgtcggtg cgggaggttt tcgaattgac cgtattgcac
     8641 aagatttccg gtttccccgt gcttgaaggc aaggttgtcg tcggtattgt gaccaaccgc
     8701 gacttgcgct tcgaaaccaa cctcgatcaa ccggtccgga acatcatgac gccgcgcgac
     8761 cggctggtga cggttaagga gggcgctacg gttgaggagg gcaaggcgct gatccacaag
     8821 catcgcctgg agcgtgtgct ggtcgtcaat gacgcgttcg aacttcgtgg tctgatcacc
     8881 gtcaaggaca tcatcaagac taccgagcat ccgctggcca gcaaggacgg ttcgggccga
     8941 ctccgcgtcg gcgcagctat tggtgtcggt gcgggcacgg cagagcgcgc agaattgctt
     9001 gccgaagccg gcgtcgatgt aatcgtggtc gataccgcac acggacactc gcaaggggtt
     9061 ctggagcgtg tgcagtgggt caagagaaac ctgccgcagg tggaggttgt tggcggtaat
     9121 attgccacgg ccgatgccgc acgcgccttg atcgatcatg gcgccgacgg tgtcaaagtg
     9181 ggcattggcc cgggttccat ttgcacaaca cgcattgtgg caggtgtcgg tgtgccccag
     9241 attacggcca ttcagatggt ttccgacgcg ctcacgaata ccggtgtgcc catgattgct
     9301 gatggcggga ttcgttattc cggggatatc tgcaaagcga ttgccgccgg tgccaatgcg
     9361 gtcatgctcg gcggattgtt tgccggcacc gaagaggctc cgggtgacgt cgaactctac
     9421 caggggcgat cctacaaatc ctaccgcggc atgggatcga taggcgcgat ggctgcggga
     9481 gcggcagatc gttatttcca ggacagtgga tcgaacatcg acaaactggt accggaaggc
     9541 attgaagggc gtgtgcctta caaaggttcc gtgttggcgg tgatccacca gttgaccggg
     9601 ggcttgcgtt cttccatggg ctatctgggt tgcgcgacga tcgaggaatt acacgagcgc
     9661 gcagaatgcg ttgaaattac tgcgtccggc gttcgtgagt cacatgtgca tgacgtgcag
     9721 atcaccaagg aagcgccaaa ttaccacgtg gactgattga ttccgaatcc ggccgggcgg
     9781 cttggagggt ttgtacctct tcgccgcctt cctttattcc aggctcgctc atgactcacc
     9841 agaaaattct catcctcgac ttcggttctc aggtttccca actcattgcc cgccgcgtgc
     9901 gtgagcaaca ggtctattgc gaactgcatc cgtttgatgt gagcgaggat tttgttcgtc
     9961 aattcaatcc acagggaatc atcctgtccg gcggaccgaa ttcggtctac gaaagtgaag
    10021 aatggcgcgc tccgcaggtc gtttttgatc tgggcatacc cgttctgggc atctgttacg
    10081 gcatgcagac catggctcag caactaggag gcaaagtcga gagctcgggc aagcgcgagt
    10141 tcggctacgc ggaggtccgc gcccgcgggc attcagaact actgaagggc ttgcaggatc
    10201 gcagcaacga tgcgggccat ggcttgcttg acgtatggat gagccacggc gacaaggtta
    10261 ccgagttgcc gcccggcttc aaggtgatcg ccagcaactc aacgtgcccg atcgctgcca
    10321 tggccgacga gtcgcgcaag ttctacgcgg tcctgtttca ccccgaagtc accaacacgc
    10381 tcaagggaaa gcagattttt tcccgcttcg tgcatgacat ctgcggctgc agaaacgact
    10441 ggacgatgcc aggatatatc gatgaagcgg tcgccaggat tcgcgcccag gttggccagg
    10501 atgaagtcat ccttggtctc tcaggcggcg tcgactcttc ggttgcagcc gcactgatcc
    10561 accgtgccat tggtgaccaa ctgacctgcg ttttcgtcga caacggctta ctgcgtctgg
    10621 atgaagccga gcaggtgatg gatacctttg cgcgcaatct tggcgtcaga gtgattcatg
    10681 tcaatgctac cgagcaattc atgagtcatc tcaagggtgt gtccgatccc gagcagaagc
    10741 gcaaaatcat tggccgcgag ttcgtcgagg tctttcaggt cgagtcgcag aaacgggcta
    10801 acgccaagtg gctggcgcaa ggaacgatct acccggatgt aattgagtcg gcgggtgcaa
    10861 aaaccaggaa ggcgcacacc atcaaaagcc accacaacgt cggcggacta ccggaaacgc
    10921 tcaatctgaa actgcttgag cccttgcgcg agctgttcaa ggacgaagtg cgcgagctcg
    10981 gcgtggcgct cggcctggca cacgaaatgg tctatcggca cccgttcccg gggccggggc
    11041 tgggggttcg catccttggc gaagtgaagc ctgaatttgc cgatcttctg cgccgcgccg
    11101 acgccatctt catcgacgaa ttgcgcgctg ccggctggta cgaaaaaacc agccaggctt
    11161 ttgccgtttt tctgccagtg aagtcggttg gcgtgatggg cgacggccgt acctatgaat
    11221 acgttgttgc cttgcgcgcc gtcgagaccc aggatttcat gaccgcgtac tgggccgaac
    11281 tgccgcacag cctgctcggc aaggtatcta atcgcatcat caacgaggtc cgcggcatca
    11341 accgggtagt gtatgacatt tccggcaagc caccagcgac gatcgagtgg gaataa
//
LOCUS       DAORPM010000048        11385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1258_length_11385_cov_11.335922, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000048 DAORPM010000000
VERSION     DAORPM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1258_length_11385_cov_11.335922"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLD53_03805"
     CDS             <1..546
                     /locus_tag="PLD53_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HRC60061.1"
                     /translation="GDGDFRPQLVYSRDIDFALASDLFGLYRHRREALQALRKIAEGH
                     RLCHNLIGIGTGSPGEACVGYKQRTCRGVCVGKEALSLHSARLMTALAKSRLSHWPYL
                     GPVALIERDEFGMREDYHLVNNWRYLGTVHDEAALYELIENRSEGPFEPEIYRIINKL
                     LKAGKTRVLPLFQALPPVSSL"
     gene            complement(538..1194)
                     /locus_tag="PLD53_03810"
     CDS             complement(538..1194)
                     /locus_tag="PLD53_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRC60062.1"
                     /translation="MKIITFLTILLLPILASAGAYEDMEEAIIQGDAPDAIALIKRGM
                     DVNTVDREGNTLLFQAVRRDIPELVDYLIRHRARLNVRNRNGETAVSIAAFKGNLSYV
                     KLLVDAGADVNLYGWSPLIYAAYNGHAQIVDYLLKRGARIDATTENGSSALFFAARFG
                     HIEVVELLLKNKADPMIANENGDTAVDWALKSENTDIADLLRAAGGRSGKAVVLELSK
                     "
     gene            complement(1191..1982)
                     /locus_tag="PLD53_03815"
     CDS             complement(1191..1982)
                     /locus_tag="PLD53_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRC60063.1"
                     /translation="MLVDSHCHLDFPKLAENLPQLLDLMQKNEVGCAVCIGVTLEDFP
                     RVLSLAETHSGTPHILATVGVHPETTDVREPTVKDLVDLAQHPKVIAIGETGLDYYWH
                     KDAPEWQRERFRTHIRAAIEVDKPLVIHNRDATTDTLRLLSEEGAGEVGGVMHCFTET
                     WDVAEAVLAQGFHISLSGIVTFKNALSIKDVARRVPLDRLLIETDSPYLAPMPYRGKT
                     NQPGYVKYVAEEIAHLRNISFEEVAKATTDNFFRLFPHARTSISQ"
     gene            complement(1993..2367)
                     /locus_tag="PLD53_03820"
     CDS             complement(1993..2367)
                     /locus_tag="PLD53_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRC60064.1"
                     /translation="MSADSKPAVAGAARPSVLSLNINSKSALYAAFMPLLRNGGIFIP
                     TTRAYNIGDEVFMLLSLMDDPAKLPIAGSVVWITPAGAQNSKAQGIGVHFKNDESGVE
                     AKRKIEGLLGGVMQSTRPTHTM"
     gene            complement(2375..3406)
                     /gene="holB"
                     /locus_tag="PLD53_03825"
     CDS             complement(2375..3406)
                     /gene="holB"
                     /locus_tag="PLD53_03825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRC60065.1"
                     /translation="MNIYDLHKEAWAQLGARRAQLPHALLISGQRGIGKFDLARHFAD
                     SLLCENPAPSSTACGKCSACGWLVQGNHPDFRLLQPDALADEDGNGVDASSKKKPSQQ
                     ITIDQVRALDDFLHVGTHRHGARVILINPAEAMNRSTANSLLKSLEEPIVNTLFLLVS
                     SEPERLLATIRSRCQSLRVRQPAPSLAESWLRQAGIGDAGHWLALAGGSPLLAVELGA
                     SEERVLLEALIAEMSRGIELDPLAAAAAIDRVVKAEKRAAPLKRMVEWSQKWLLDLTL
                     ASEELPVRYFLTQEQLLRQLAKSTDTPNLLAFDRKTLQYKLQCEQPLNSRLFLEEFFL
                     NYAALFRTS"
     gene            complement(3403..4032)
                     /gene="tmk"
                     /locus_tag="PLD53_03830"
     CDS             complement(3403..4032)
                     /gene="tmk"
                     /locus_tag="PLD53_03830"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRC60066.1"
                     /translation="MSPSNKRGKFITFEGIDGAGKSSHIAGVVELLRAHGLNVIATRE
                     PGGTPLGEKLRELLLNKAMHPETETLLMFAARREHLAQVIDPALARGDWVVCDRFSDA
                     TYAYQGGGRGLDKNKFADLEQWVHGHLQPDLTFLFDLPPSVAGERITAQGRELDRFEQ
                     EQADFHIRVRLAYLERSAASPQRIQVINADQPPEIIKKQVEDTVITRCL"
     gene            complement(4044..5042)
                     /gene="mltG"
                     /locus_tag="PLD53_03835"
     CDS             complement(4044..5042)
                     /gene="mltG"
                     /locus_tag="PLD53_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRC60067.1"
                     /translation="MLKLIKKLITLGLILVVVLGGGFFWLVSSALDLKSEQVDFHIAR
                     GSSMRIAANEIASAGVPLHPWVLIVLGKALHVENSIKAGSYEINRGITTLELLKKLTR
                     GDVKQTEIAFIEGWTFSQMRERLDAHPDVRHESKGLPDSEIMRLIGAQEGAAEGLFFP
                     DTYLFAKQSSDIDVLSRAYRTMQRHLAREWEGRAGGLPYKNAYEALIMASIVEKETGR
                     DQDRPLVAAVFANRLRLGMLLQTDPTVIYGVGEMFDGNLRKSDLLNDTPFNTYTRAGL
                     PPTPIAMPGLASLRATLHPAPSQALYFVARGDGSSVFSQTLDEHNQAVNRYQRGGK"
     gene            5253..6269
                     /locus_tag="PLD53_03840"
     CDS             5253..6269
                     /locus_tag="PLD53_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HRC60068.1"
                     /translation="MNSNWQEFLGTSGARTGSGMLADFGDIPAELAAARDATIVSPLT
                     HLGLIECAGDDAKAFLHNQLTSDINHLAPGFAQHSAWCTAKGRMLASFLLYRNGADYR
                     ALLSCDLVSTIQKRLQMFVLRSKVKVLDLSGTHLVFGLSGPQAEAALQSAGFSAPASA
                     LQTAVFADGAVIRLDGARLILVVANDVAMTTWKKITSIALPASTPVWQWLDVQAGVPL
                     ITEATKEAFVPQMANFDKIDAVSFHKGCYPGQEVVARTQYLGKVKRHLYRIHVNGMVA
                     PGTAIHASGSPEQPCGTVSNAAPAPGGGCDALAVIQESFVETGALHLDMPDGLIVAIE
                     PVVS"
     gene            6293..7066
                     /locus_tag="PLD53_03845"
     CDS             6293..7066
                     /locus_tag="PLD53_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HRC60069.1"
                     /translation="MCLILLAWQSHPDYPLVVAANRDEFFSRPAAPAGFWAESPAILA
                     GKDLEAGGTWLGVSRQRRFAALTNYREGGHQLSNAPSRGALVADFLISESDPETYLES
                     LASRRENYNGFNLLFGDSRRLAYFTNRGDSPRWLAPGIYGLSNHLLDTPWPKLSDARA
                     AFSNALKRLPDSAPFFELLSDQEIVPDIHLPESGVPIEWERILSAVFVNSPTYGTRAC
                     TLLTVHRSGLATLVERSFGPAALPLGEVSVAFESSIFGS"
     gene            complement(7071..7553)
                     /locus_tag="PLD53_03850"
     CDS             complement(7071..7553)
                     /locus_tag="PLD53_03850"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRC60070.1"
                     /translation="MHIHISIARQLLTLIDGVGQVIRSYPVSTASRGAGEQYGSFCTP
                     RGKHVIRARIGAGQPVNTVFVARRPTGEIYTEDLGRAFPDRDWMLTRILWLSGCEPGR
                     NRLGCVDSMRRFIYIHGSPDTATMGAPGSHGCIRMRNADILQLFDLVPPYTPVEITED
                     "
     gene            complement(7556..8017)
                     /gene="tadA"
                     /locus_tag="PLD53_03855"
     CDS             complement(7556..8017)
                     /gene="tadA"
                     /locus_tag="PLD53_03855"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRC60071.1"
                     /translation="MQDEYYMREALSLARSAECLGEVPVGAVVVRDGVIVGRGFNSPI
                     GESDPTAHAEIAALRDAARALENYRLPGCELFVTLEPCAMCAGAILHSRIARVVYGAR
                     DLKTGVHGSVVDLFAVERLNHHTQVVGGVLADECGRMLSAFFAGRRVKGEQ"
     gene            complement(8134..9093)
                     /locus_tag="PLD53_03860"
     CDS             complement(8134..9093)
                     /locus_tag="PLD53_03860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRC60072.1"
                     /translation="MSSSPDSPPNSPPGCPADCPPLPEGYQLYGYRIERMLSRGGFSI
                     AYMASDSAGTPVVVKEYLPIQMARRAAGSQALIVSSASQGKFNHGMRSFLEEARLLAR
                     ISHPNIVAVLDFSKANNTSYIVMRYEDGRSLEEYLHRLRESGRKIREDLLRNVFVRLL
                     SGLREVHSQKLLHLDLKPANIYLRKDGHPVLLDFGAARWGLGSPDGSLGNIYTRGYAA
                     PEQQGSGEGMGPWTDIYAIGATLYACLDTGQAPQAADLRMNADEVEPAQRRWAKAYSL
                     QLLELIDWCMQIPVRARPQSVYALQKVLNGELLDLVDPAWFRK"
     gene            complement(9196..9681)
                     /locus_tag="PLD53_03865"
     CDS             complement(9196..9681)
                     /locus_tag="PLD53_03865"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HRC60073.1"
                     /translation="MIIFDLSCKQEHPFEGWFQSLSSYDSQIEAGLISCPHCGSTEIR
                     RVPSVVHMSKPAKSAHPTEPKNSSDPARHAGTPGSSTGLPATRTQAEMFAAFEQLMSS
                     IVSNSEDVGQNFAEEARRIHYLEAPVRSILGEASDEEYESLLDEGIEVLRLPIVKKSI
                     N"
     gene            complement(9894..10112)
                     /locus_tag="PLD53_03870"
     CDS             complement(9894..10112)
                     /locus_tag="PLD53_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60074.1"
                     /translation="MQITIESPGGPRQGVVPSDGIVDEATLIKALILTLAVEGNKGVD
                     YVTLEVDLSDAEPERLVEVAKALGNKGH"
     gene            complement(10265..11263)
                     /locus_tag="PLD53_03875"
     CDS             complement(10265..11263)
                     /locus_tag="PLD53_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRC60075.1"
                     /translation="MASETFLASFGQIGLRLIERLGISPQEFTRPFGLDVKALPGPKA
                     RLPVRFGDETFARIASLVPDPAFALHSAECWHPSNLGTLGYAWLSSETLRAALARLTR
                     YAKIVGEKATCRCMDRPNGLHLIYESMRGNSLVGYVMTDFAMAIIVDMCRTNFGPSLA
                     LESVYLRRPRPVDSRPYEDFYGCPVTFGEAEDGFVLGRHVADKPLPTANQELATTFDA
                     ILAAELAKLGSSDIEARARAYLLEQLTSGEPSEALLAREMGMSSRTLQRKLGELELTY
                     RSVLEKTRYDLALRYLDDPAKTITDITFLLGFSEQSAFARAFKRWSGKTPSSCRRR"
BASE COUNT         2456 a         3264 c         3162 g         2503 t
ORIGIN      
        1 ggcgatggtg attttcgccc gcagctcgtc tattcacggg atattgattt cgcgcttgct
       61 agcgatttgt ttggtctgta cagacaccgg cgcgaggccc tgcaagccct gcgcaaaatc
      121 gctgaaggac atcggctgtg tcacaacctg atcgggatag gcacgggatc tccgggagaa
      181 gcctgtgtgg gctacaaaca aaggacctgt cgaggcgttt gtgtgggcaa ggaggccttg
      241 tccttgcaca gcgctcgact gatgacggct ttggcaaaat ccaggctaag tcactggccg
      301 tacctcgggc cggtagctct gattgaacgc gatgaattcg ggatgcgcga ggactaccat
      361 ctggtcaata actggcgcta tctgggtacg gtgcatgacg aagcggcact ttatgaactc
      421 attgaaaacc gaagcgaagg ccctttcgag cctgaaattt accggattat caacaagtta
      481 ttgaaggcag gcaaaacccg agtcctgcct ctttttcagg ctttgccgcc agtttcgtca
      541 ctttgagagt tcaagaacca ctgccttgcc cgagcgccct ccagcggcac gaagcaaatc
      601 ggcaatatcg gtgttttcgc tcttcaaagc ccagtcaacc gccgtgtccc cattctcatt
      661 ggcgatcata gggtctgcct tgttcttcaa aagcaattcg acgacttcga tgtgaccaaa
      721 gcgtgcagca aagaaaaggg ctgaagaacc attttctgtt gtggcatcta tccgggcgcc
      781 gcgtttcaac aggtagtcga caatctgggc gtgaccattg tatgcggcat agatcagagg
      841 cgaccagccg tacaaattca catcggcacc ggcgtcgaca aggagtttca cataagagag
      901 attccctttg aatgccgcaa tgctgaccgc agtttcgcca ttccgattgc gcacattgag
      961 tcgtgcacga tgccggatca ggtaatcgac gagttccgga atgtcgcgcc gaacggcctg
     1021 gaaaagcagc gtgttacctt ctctgtcgac ggtattgacg tccatgccgc gcttgatcag
     1081 agcaatggcg tcgggggcat cgccctgaat tatcgcctct tccatgtctt cgtaagcacc
     1141 tgccgaagcg agtatcggca acagcaaaat ggtcaggaaa gtaatgattt tcattggctt
     1201 atactcgttc tggcgtgagg aaaaaggcgg aaaaagttgt cggtcgtggc cttggcgact
     1261 tcttcaaatg agatattccg caagtgtgcg atttcttcgg caacgtattt tacgtagccc
     1321 ggctggttgg ttttcccgcg ataaggcatg ggtgccaagt agggggaatc ggtttcgatg
     1381 agcaagcgat ccagaggaac tcgacgagca acgtccttga tggatagcgc gttcttgaac
     1441 gtgacgatac cggacagtga aatatggaac ccctgggcga gcactgcttc ggccacatcc
     1501 caggtctctg tgaagcaatg catgacgccg cccacctcac ctgcgccctc ctccgacaaa
     1561 agcctcagag tgtcggtcgt agcatcccga ttgtggatga ccaatggctt gtcgacttca
     1621 attgcagcgc ggatgtgggt gcgaaaacgt tcacgctgcc attccggtgc atccttgtgc
     1681 cagtaatagt cgagtccggt ttcgccgatc gcgatgacct tcgggtgttg tgccaaatcg
     1741 actaggtctt taaccgtcgg ttcgcgcaca tcggttgttt caggatggac tccaaccgtt
     1801 gccagaatgt gtggcgtgcc tgaatgcgtc tcggcaagcg acaagactct gggaaaatct
     1861 tccagggtga ctccgataca aaccgcgcag cccacttcgt tcttctgcat caggtcaagc
     1921 agttgcggga gattttcagc tagtttcggg aagtcaaggt ggcagtgtga atcgacaagc
     1981 atcagacggt cactacatgg tatgagtcgg acgagtggat tgcattacgc cgccaagaag
     2041 gccttctatc ttgcgttttg cttcaacgcc gctttcgtcg ttcttgaaat gcacgccaat
     2101 tccctgcgct ttactgttct gggcgcctgc cggtgtgatc cagacgaccg atccagcaat
     2161 cggcagcttg gcggggtcat ccatcaggga caacagcatg aatacctcgt caccgatgtt
     2221 gtaggcacgc gtcgtaggga tgaagatgcc cccattgcgc agtaagggca tgaaggcggc
     2281 gtagagtgcc gacttcgaat tgatgttcag cgagagtacg ctcgggcgtg ctgcgccggc
     2341 aacggcaggt ttggaatcgg ctgacatatc cgcctcagga agtccggaac aaggccgcgt
     2401 aattcaggaa gaactcttcg agaaatagac ggctgttcaa aggttgttcg cattgcagtt
     2461 tgtattgtag cgttttcctg tcaaacgcca acaaattagg ggtatcggtt gatttcgcca
     2521 gttggcgcaa cagttgctcc tgcgtgagga aataacgcac ggggagttct tcgctggcca
     2581 gcgtcaggtc caatagccac ttttgcgacc actcgaccat tcgtttcaag ggtgcagctc
     2641 gtttctcggc ttttacgacc cgatcaattg cggcggccgc cgccagagga tcaagctcga
     2701 taccacgtga catttcggca atcaaagctt cgagcagcac gcgctcttca ctcgcaccaa
     2761 gttcgacggc caacagcggt gagcctccgg ccagcgccag ccaatgcccc gcgtcgccga
     2821 tgcctgcctg tcgtaaccag gattcagcga ggctaggagc cggctgtcga acacgaagcg
     2881 actgacagcg gctacggatt gttgccagca atctctcagg ctcactggaa accaataaaa
     2941 acaacgtatt tacaatcggt tcttcgagtg atttaagaag cgagtttgcc gtcgaacgat
     3001 tcatcgcttc cgcgggattg atcagaatca cgcgcgcgcc gtgacgatgc gtcccgacat
     3061 gaaggaagtc gtccagggcg cgaacctggt cgattgtgat ttgctggctc ggtttcttct
     3121 tgctgctcgc atcgacgcca ttcccgtcct catcggccag tgcatccggt tgcagcagac
     3181 ggaaatccgg gtgattgccc tgcactagcc aaccgcaggc agagcattta ccgcaggctg
     3241 tcgacgaagg tgccgggttt tcacagagta gggagtcggc aaagtgtcgc gcgaggtcga
     3301 acttgccgat tccacgctgg cccgaaatca gaagcgcgtg cggaagttgt gcgcgtctag
     3361 ccccaagttg cgcccaagct tctttgtgaa ggtcataaat attcataaac agcgtgttat
     3421 aactgtgtct tcaacttgtt tcttgattat ttccggcggc tggtcggcat tgattacctg
     3481 aattcgttgc ggtgatgccg ccgaacgttc aaggtaggcc aagcgtacgc ggatgtggaa
     3541 gtcagcctgc tcctgctcga aacgatcaag ttcgcgtcct tgcgccgtaa tacgttcgcc
     3601 ggccaccgag ggtggcaagt cgaaaagaaa ggtcaaatcg ggctgcagat gcccgtgcac
     3661 ccactgctcc aggtcggcga acttgttttt gtccaggccc cgccccccgc cctgatacgc
     3721 ataggtggcg tcgctgaatc ggtcgcagac gacccagtca cctcgcgcaa gtgccgggtc
     3781 aattacttgc gccaggtgtt cgcgtcgcgc cgcgaacatc agcagggtct cggtctcggg
     3841 gtgcatggcc ttgttcaaga gcaattcgcg aagtttttcg ccaagcggtg tgccgccggg
     3901 ctcacgggtt gcgattacgt tcagcccatg tgctcttagc aattcaacta cgccggcaat
     3961 atgactgctc ttgccggcac cgtcgatgcc ctcgaaagtg atgaacttcc ctcttttatt
     4021 gcttggggac attgtttccg gcttcacttc ccgcctctct gatagcgatt taccgcctgg
     4081 ttatgctcgt ccagcgtttg cgaaaacacg ctgctcccgt ccccgcgcgc cacaaaatat
     4141 agcgcctgac ttggtgccgg atggagcgta gcccgaagtg aggccagacc aggcatggca
     4201 atgggtgtag gcggcaaacc ggcgcgcgta taggtattga aaggcgtgtc gttgagaaga
     4261 tcacttttgc gcagattgcc atcaaacatc tcaccaactc cgtaaatcac ggttggatcc
     4321 gtctgcaaca gcatacccag ccgcaggcgg ttggcgaaga cggcggccac caacggccga
     4381 tcctgatcac gcccggtttc cttttcaacg attgaggcca tgatcaaagc ctcatacgcg
     4441 ttcttatagg gtagtccgcc cgctctgccc tcccactcac gcgcaaggtg acgttgcatg
     4501 gtccgatacg cgcgtgacag cacgtcgata tcgctgctct gcttggcgaa aaggtaggtg
     4561 tcggggaaga acaagccttc tgcagctcct tcctgggcac cgatcaggcg catgatctct
     4621 gaatccggca agcctttact ttcatgacgg acatcgggat gcgcatcaag tcgttcccgc
     4681 atctggctga aggtccagcc ttcgatgaaa gcgatctctg tttgtttgac atcgccgcgc
     4741 gtcagtttct tcaacaattc cagcgtggtg atgccccggt tgatttcgta actccccgct
     4801 ttgattgaat tctcgacatg caacgccttg ccgagaacaa ttagcaccca gggatggagc
     4861 ggcacaccgg cgctcgctat ctcattggcc gcaatccgca tgctactgcc ccgagcaatg
     4921 tgaaaatcga cctgctcgct cttcaagtcc agcgccgagg atacaagcca gaaaaacccg
     4981 ccacccaaca caacgacaag aatcaggcca agggtgatca gtttttttat cagtttaagc
     5041 atctatccgg cgcgatcggg gcctcgtggg tctgcaatgg gaatttgggt ggcgcaacgt
     5101 gcgctatata atagccgaaa cccgtatcaa tgaaccgaag cagcacttgg gggtcgaagc
     5161 cgcagcgaag tcgcacagtt cctccgagga gaattccctt tattcggaca ggcaatccgt
     5221 ggctcaacaa taacgatgga tataccgtaa cgatgaactc caactggcaa gaatttcttg
     5281 gcacttccgg cgcccgcacc ggcagcggaa tgcttgccga tttcggtgac atccccgccg
     5341 aactcgcggc cgcacgcgat gcgacgattg tttcgcctct tacacactta ggattgatcg
     5401 aatgcgcggg cgacgacgca aaagccttcc tccacaacca gttgaccagc gacatcaacc
     5461 atcttgcccc cggtttcgcc cagcattccg cctggtgcac ggccaaagga cggatgctgg
     5521 cttcttttct cttgtatcgc aacggcgcgg actatcgcgc attgttgtcc tgcgatctgg
     5581 tatcgactat ccagaagcgc ctgcaaatgt ttgtgttgcg ctccaaggtc aaggttctcg
     5641 atctttccgg cactcacctg gtcttcggct tgtccggccc gcaagcggaa gctgcattgc
     5701 agagcgccgg tttttctgcg cccgccagtg cactgcaaac cgcggttttt gccgatggtg
     5761 cggtcattcg acttgacggt gcacgtctga tcctcgtcgt tgccaacgat gtcgcaatga
     5821 caacctggaa gaaaattacc tcgatcgccc tcccggcaag cacgcccgtg tggcaatggc
     5881 tcgatgttca ggcgggagtt cctttgatta cggaggccac caaagaggcc tttgtgccgc
     5941 aaatggccaa tttcgacaag atcgatgccg tcagcttcca caaaggctgc taccccggcc
     6001 aggaagtcgt cgctcgcacg cagtatctcg gcaaagtaaa gcgtcacctc taccgtatcc
     6061 acgtgaacgg catggttgcc ccaggaacgg caattcacgc ctcaggcagc cccgagcaac
     6121 cctgtggcac ggtctccaac gcggcgccag cgcctggagg cggctgtgat gccttggccg
     6181 tcattcagga aagttttgtc gaaacaggtg ctctgcacct ggatatgccg gatggtctga
     6241 tcgtcgctat tgagcctgtc gtctcctagc tgccagcctc ggttcggttt cgatgtgcct
     6301 catcctcttg gcctggcaga gtcatcctga ctatccgctg gtcgtggccg ccaaccgcga
     6361 tgagttcttt tcccgacctg cggcgccagc cgggttctgg gcagaatccc cggcgattct
     6421 cgccggcaaa gatctggaag cgggtggaac ctggctgggt gttagccgcc agcggcgttt
     6481 tgccgcactg accaattatc gcgaaggtgg gcatcagctc agcaacgcgc cttcccgcgg
     6541 cgcactggtg gctgatttcc tcatcagcga aagcgatccg gaaacatatc tcgaatcact
     6601 ggcaagccgc cgagaaaatt acaacggatt caacctgttg ttcggtgata gtcgccggct
     6661 ggcgtatttc acgaaccggg gcgatagtcc gagatggctc gcgccgggaa tctacggcct
     6721 gtccaaccat ttgctcgaca cgccgtggcc gaaactatcg gacgccagag ctgcgttttc
     6781 caatgcattg aagcggttgc cggattcggc cccgtttttc gaactgctct ccgaccagga
     6841 aatcgttccc gatatccacc tgccggaatc tggagtcccc attgaatggg agcgaattct
     6901 gtcagccgtc ttcgtgaatt cgccgaccta cgggacgcgt gcgtgtaccc tgctgacggt
     6961 gcatcgaagt ggtctggcca cactggtgga acggagtttt ggtccggctg ccctgccgct
     7021 tggtgaggtg agcgtggctt tcgagtcctc gatatttggt tcctgaaaaa tcagtcctca
     7081 gtgatttcca ctggagtata cggcggcacc aggtcgaaca actgaaggat gtcggcattg
     7141 cgcatgcgta tgcagccgtg cgacccgggc gctcccatgg ttgccgtatc cggactgccg
     7201 tggatataga tgaaacgacg catgctgtcg acgcacccaa gccggttacg ccccggctca
     7261 caccccgaca accacaatat gcgcgtgagc atccagtcac ggtccggaaa ggctctgccg
     7321 agatcctcgg tatagatttc accggtgggg cgtcgcgcaa caaaaacggt gtttaccggt
     7381 tgccctgcac cgattcgggc acggatcaca tgcttcccgc gtggcgtgca gaaactaccg
     7441 tactgttccc ctgccccccg gcttgccgtg gaaaccggat agctccgaat cacctgccct
     7501 accccgtcga tcagggtcag taattgtcgc gcaatggaaa tatggatatg caactctact
     7561 gttctccctt gacgcgacgt ccggcaaaga aagctgaaag catacggccg cactcgtctg
     7621 ccagtacgcc acccaccacc tgagtatggt gattcaggcg ctcaacggcg aacaggtcta
     7681 caacacttcc gtggacgccg gttttcaggt cgcgggcacc gtacaccaca cgggcaatgc
     7741 gcgagtgaag aatggcaccg gcacacatcg cacacggctc aagcgtcaca aacagctcgc
     7801 agccaggtaa tcggtagttc tccagcgcac gcgccgcatc tcgcagggcc gcgatttcgg
     7861 catgggctgt cgggtcgctt tccccaatcg gcgagttgaa acctcgcccg acgataacac
     7921 catcacggac caccacggcg cctacgggca cctcgccaag gcactcggca gagcgcgcca
     7981 gggacagcgc ctcgcgcata tagtactcat cctgcatcgg tcttcatcat tccgtgtcag
     8041 aaaccgctca gattatcgtg cgattcatac cccgtacagc ttaatcccgg ataccaaaga
     8101 atgcaccaaa ggcattctca tctacggatg gcactacttc cggaaccaag ccggatcgac
     8161 aagatcaagc aattccccgt tgagcacctt ttgcagtgca taaacgcttt gtggcctggc
     8221 cctgaccgga atctgcatgc accagtcgat caattccagg agttgtagcg agtaagcttt
     8281 tgcccaacgc cgctgggcag gctccacctc gtcagcattc atccgcaagt cggctgcctg
     8341 aggtgcttgt cctgtatcga ggcaggcgta caaggtggcg ccgatcgcgt agatatcggt
     8401 ccatggtccc atgccctcgc ccgaaccctg ctgttccggg gcggcatagc cccgggtgta
     8461 aatattaccg agtgagccat cgggcgatcc caagccccag cgcgcggcac caaaatcgag
     8521 cagaaccggg tggccgtctt tgcgcaagta gatattggca ggcttcaggt caagatggag
     8581 cagcttctgg ctatgaactt cgcgcagacc agaaagtaaa cgcacaaaga cattgcgcag
     8641 aagatcctcg cgaatcttcc tgccgctctc acgtagccgg tgcaaatatt cttcgaggct
     8701 tcgaccgtct tcatagcgca tcacaatgta ggacgtattg ttggccttcg aaaagtccag
     8761 tacggcaacg atgttgggat ggctgatacg agccagtaac cgcgcttctt caaggaaact
     8821 gcgcatgccg tggttgaact tgccctggct tgctgaagaa acgatcagtg cctggcttcc
     8881 tgccgctcgc cgggccattt gtatcggcag gtattccttg accacaaccg gcgtcccggc
     8941 actgtcgctg gccatgtaag caatggaaaa gccccccctc gacagcatgc gctcgatccg
     9001 ataaccgtag agctggtagc cttccgggag cggggggcaa tcggctgggc aaccgggcgg
     9061 agaattgggc ggagaatcgg gcgatgatga catgctttga tccatgttca accctcaagt
     9121 gcaccgggcc tcgccttcag ccactgactt catcatgccg atgaaatctg ccgagcaaga
     9181 ttccagagaa ctgcgtcagt taatgctctt cttgacgatc ggcagacgca gtacttcgat
     9241 gccttcgtca agcaaagact cgtactcttc atcgctggct tcaccgagga tcgaacgcac
     9301 aggcgcttca agatagtgaa tcctgcgcgc ttcctcggca aagttctggc caacgtcttc
     9361 gctgttcgag acgatcgacg acatgagctg ttcaaaggcg gcgaacattt cagcctgtgt
     9421 gcgggttgca ggcaatccgg tagaggaccc gggcgtgcca gcatgacgtg ctgggtccga
     9481 tgagttcttt ggctccgttg gatgcgccga ttttgccggt ttcgacatat gcacgacaga
     9541 cggtacgcgc cgtatctcgg tagagccaca atgcgggcac gaaatcaagc cggcttcgat
     9601 ctggctatca taggatgaca gcgattgaaa ccagccttcg aagggatgtt cctgtttgca
     9661 ggacaagtca aatatgatca tcagaaaagt aggctgttag gggtgcggag aaggagcgga
     9721 atcgaacagg cgtgcgagaa gcctaatgag ggattccaga caggcaatac gcgtagttta
     9781 ccagatcagg ccagactcct cggcatgaat cggaaatcga ccgccctgcc cgacggttca
     9841 acccgaatgc tgccgaccat cagactcgaa cagattggcg gcgcgagggc cagtcagtgc
     9901 cctttgttgc ctagcgcctt ggcgacctcg acaaggcgct ccggttccgc atcgctcaga
     9961 tcaacctcca gcgtcacgta gtcgacaccc ttgttccctt caacggcaag cgtcaggatc
    10021 aaggctttga tcaatgtggc ctcgtcgaca atgccatcgg aaggcactac gccctggcga
    10081 ggtccgccgg gggattcaat cgtaatttgc actcacagcc ccagtaaaaa gtcctcaatg
    10141 gagcgtagct tctgacgtgt caatacgatg gaccgatagc gccaatctct tgactgaagg
    10201 cgtcagaatt ttgaacttta cgattgaaac ttggtgggag attgcctggg cagcactgca
    10261 cccgctaacg acgacgacaa gaactggggg tctttccgct ccagcgtttg aatgcgcgtg
    10321 cgaaagcgct ttgctcggag aaaccgagca ggaaggtgat gtcggtgatg gtcttggcag
    10381 gatcatcaag atagcgcaat gccagatcgt atcgggtctt ctccagcaca ctcctgtacg
    10441 tcaactccag ttcgccaagt tttcgttgca acgtgcgcga actcataccc atttcgcgcg
    10501 ccagtaacgc ttccgacggc tcaccggagg tcaattgctc cagcaaatac gctctggcac
    10561 gcgcctcgat gtcgctgctg ccgagcttcg cgagttccgc cgccaggatt gcatcgaatg
    10621 tcgtcgcaag ctcctggttg gctgtcggga gcggtttgtc tgccacgtgc cgtcccagca
    10681 caaatccatc ttccgcctcg ccaaaagtga ccgggcagcc atagaaatcc tcgtagggtc
    10741 ttgagtcgac cggccgcgga cgtctcaaat agaccgactc gagggcaaga gaggggccaa
    10801 agttcgtccg gcacatgtcc acaatgattg ccatcgcgaa atcagtcatg acgtagccca
    10861 ccagcgaatt cccgcgcata ctttcataga tcagatgaag gccgttcggg cgatccatac
    10921 atcgacacgt cgccttttcg cccacgatct tcgcgtagcg cgtcaggcgc gcaagcgccg
    10981 cgcgcagggt ttcgctggag agccacgcat agcccagagt gccgagattt gaagggtgcc
    11041 agcattcagc cgaatgcaat gcaaatgccg ggtctggaac cagtgaagcg atcctggcaa
    11101 aggtctcatc gccaaatcgt acggggaggc gggcctttgg gccgggcaaa gcctttacgt
    11161 cgagaccaaa ggggcgcgtg aattcctgtg ggctgatgcc aagccgttcg atgaggcgca
    11221 gcccgatttg tccaaaactt gcgagaaatg tttccgatgc catgatcacc ccgcctttag
    11281 ctcccaggtt cgattatgtc gtatttggtc atgcagttgg cgctgagggt caagttgggt
    11341 agccatccct tcactagaat cggtgcatag agtcggtagt tcctg
//
LOCUS       DAORPM010000049        11294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1271_length_11294_cov_12.175727, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000049 DAORPM010000000
VERSION     DAORPM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1271_length_11294_cov_12.175727"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /gene="ccsA"
                     /locus_tag="PLD53_03880"
     CDS             complement(<1..401)
                     /gene="ccsA"
                     /locus_tag="PLD53_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRC60076.1"
                     /translation="MSNKLINWFKFASPQAFYPLAGRMIPWFWTLAAIFGITGLYIGF
                     LVAPTDAQQGEGYRIIFLHVPASWMSMFIYLVMAFWAGLGLAFNFRLSGMMATALAPT
                     GALFAFLSLWTGALWGKPMWGTWWVWDARLTS"
     gene            complement(401..1069)
                     /gene="ccmB"
                     /locus_tag="PLD53_03885"
     CDS             complement(401..1069)
                     /gene="ccmB"
                     /locus_tag="PLD53_03885"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRC60077.1"
                     /translation="MLALIRAIVGRDLRLALRRRSDIVSALFFFIIVVSLFPLGVGPE
                     PELLRTLAPGVLWVAALLSTLLSLPRLFADDHRDGTLEQLALAPQPLGLIVLGKVFAH
                     WLVSGLPLTLLAPVLGIQFDLSSEALWTLVISLLIGTPALSGIGAIGAALTLGVRGSG
                     VLLSLLVLPLYIPVLIFGAGAVDAVVTGLGAQAHLSLLGALTLGGVFFAPWPTAAALR
                     IALE"
     gene            complement(1140..1754)
                     /gene="ccmA"
                     /locus_tag="PLD53_03890"
     CDS             complement(1140..1754)
                     /gene="ccmA"
                     /locus_tag="PLD53_03890"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HRC60078.1"
                     /translation="MLEASNLECVRGERSLFSKLNFRLDGGEMLDLQGKNGSGKTSLL
                     RMLCGLSPPADGEIRWRGELIGKLGEEFRRELCYLGHFNAIKEELTPLENLLSSARLA
                     GEHLDEVAALDALEIVGLAGREDLACRYLSQGQKRRVALARLVNERRALWVLDEPYVA
                     LDTAAVELVAGLIGTHLQRGGLAVLTTHQAVEVVAGTVRELRLN"
     gene            2195..4501
                     /gene="hypF"
                     /locus_tag="PLD53_03895"
     CDS             2195..4501
                     /gene="hypF"
                     /locus_tag="PLD53_03895"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HRC60079.1"
                     /translation="MQTSDNSHNVCQRIRLSGVVQGVGFRPFVWRLAKELLLTGWVRK
                     DARGVEIEVCGAAEQVQNLIERLQNDAPVKAHIDSVTAQNSHVECVADDFYILNSRSG
                     RAPAMVGHDSAVCRDCLAEMFDPANRRWRYAFTNCTNCGPRYTIARALPYARERTSLK
                     NFPRCPKCQNETQRKDGRHHNDEVNCCPKCGPQPELLDAEGRPLPGDPITQAVALLAQ
                     GKIVAIKGMGGFHLACDARNAVAVAVLRERKQRDERPFPVMLANASSASAFVQLGIGE
                     PGLLAMAERPIILLKKRPSCDKALPGVTTGLGWLGVMMPFTPLQYLLFHESAGRPQGL
                     GWLDRPQKLALVMTSADPGGEPTAISNEEALLRLSGIADAFLLHDREIKSRCDDSIAR
                     SGPGGLQLVRRARGYAPNAIKLPRSGPSVLAVGGWFKNTVCVTRGDEAFVSQHIGDLD
                     NAAACDYFESTVTRLLKLLGIKPALVAHDLDRSFHSTRFALDFAQQRGVPALAVQHHH
                     AHTAAVQAEHRIDAPVIGLSLDGIGLGADGGGWGGELLRVDGVSMERIGSLAPIELAG
                     EQALRVPWRLAAAVLQQLGRGKEIEKRFPEQRSAAAVEKMLLQGITCPKTSSLGCYIN
                     AAAGLLGISSMMAFDGQAAELLEGMADRYGDVLPLNGAWSIENGCLNLQPLLAVLADE
                     KIPERGAAIFHATLVAALTDWVCAIAPSGSKIVGSGGCFLNQVLARGLRARLGGRGLQ
                     LIEARRLPPNDGGLAVGQAWVALQYLLG"
     gene            complement(4508..5545)
                     /locus_tag="PLD53_03900"
     CDS             complement(4508..5545)
                     /locus_tag="PLD53_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRC60080.1"
                     /translation="MTGKKRNALPELLLVLVFLSACSSQIPRATDGPSPGNQSTSPAR
                     KPGVIQKHGGGYYKDDGPGDDIPDNLDDIPDAQPRSEPLHRFANRPYNVLGKSYIPDT
                     SLKHFRQSGIASWYGKKFHGQKTSIGEPYDMFSMTAAHPTLAVPSYVRVRRVSNGKTV
                     VVRVTDRGPFHADRIIDLSYAAAHRLGYINDGSTQVEVETVLPGDSTELTYAQINPPA
                     QTAKSPVPAAQRDVIEILATRADGDEASDQSPPSGASARLPVSASAPPVATTGVFLQL
                     GAFSNADNAENLRSHLSRELDWLQEGIRINAGGGMYRVHLGPYASRADAEKVAEKIRL
                     ALGYKPGFVAR"
     gene            complement(5572..6678)
                     /gene="rodA"
                     /locus_tag="PLD53_03905"
     CDS             complement(5572..6678)
                     /gene="rodA"
                     /locus_tag="PLD53_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRC60081.1"
                     /translation="MFQQILNRFWVRLTGNIDGPLLAIALVLMAFGIATVYSATYDSN
                     NRATGQLLNLGIGVAVMWALAQTPPQKLMRFAVPLYIVGVILLILVFLVGVKVNGARR
                     WLPLGFTRIQPAEILKIAMPLMLAWYFHKYESGLRFRHFVIAGLLLLIPFGLIAKQPD
                     LGTAILVGASGFYVIFFAGLPWKAIIGMIVAGASVAPFAWTMLHGYQRKRILTLIDPT
                     TDPLGSGYHIIQSTIAMGSGGRFGKGWLEGTQTRLEFIPERHTDFIFAVFSEERGLLG
                     NSILLSLYLLLIARGLMISANASTLFARVLGGSITLSFFTYAFVNMGMVSGILPVVGV
                     PLPFMSYGGTALVTLFLGLGILMSIHSHRMLVKK"
     gene            complement(6682..8583)
                     /gene="mrdA"
                     /locus_tag="PLD53_03910"
     CDS             complement(6682..8583)
                     /gene="mrdA"
                     /locus_tag="PLD53_03910"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRC60082.1"
                     /translation="MRTRYSPQNTHDSDIERFRFRVVIAASVVVLGFAILFARFFYLQ
                     VIQHDYYATRAEDNRISLVPIAPNRGVIMDRNGIVLARNYTAFTLEITPSKIENLDAM
                     IEDLGKLVEIESKDRRRFRKLLDESRSFESLPIRTRLSDEEVARFAANRYRFPGVEVK
                     ARLFRQYPLGSRASHAIGYINRINKTDLEIIEKNEQAANYRGTDHIGKTGLEQKYEFQ
                     LHGETGYEEVEIDAGGHAIRSLSRTPPVSGNNLTLTLDAKLQEITEQAFGDRRGALVA
                     IEPSTGGILALVSTPTFDPNLFVDGIRSDEWDLLNNSPDRPMVNRALNGAYPPGSTFK
                     PFMALAALEIGKRTAGQTISDPGFYNFGGHHFRDDKKGGHGTVDMYKSIVHSCDTYYY
                     MLANDLGIDNIARFMGSLGLGSRTGIDIEGESEGVLPSPEWKRRRFKSREQQKWYAGE
                     TISIGIGQGYNAYTPIQLAQAIAVVANDGVFYRPHLVRYITDTKTGEKVLIEPEPLRT
                     LAWKPENIEIIKKAMVGVNKEGTGARAFAGAEYVSGGKTGTAQLFSLKGAEYRAGKLK
                     KELHDHGLFIAFAPADHPKIALAVLVENGGFGSQSAAPIARMVLDYYLLGKLPKGAAK
                     EDDTPEEEN"
     gene            complement(8595..9116)
                     /gene="mreD"
                     /locus_tag="PLD53_03915"
     CDS             complement(8595..9116)
                     /gene="mreD"
                     /locus_tag="PLD53_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRC60083.1"
                     /translation="MQPTSSSSRILQPVRPWYILITLLAALLLNFLPTSAWPWMPDWA
                     ALLLIFWSIREQRYVGMGSGFIFGLAMDVADSSLMGQHALAYVLAIYAASALSRRILW
                     FPLGQQALHILPLLLLVQLVQFGVRAMPGAILPGLSFFAGPFVGALLWLPLTFILLLP
                     QYQPVDRDANRPI"
     gene            complement(9121..10050)
                     /gene="mreC"
                     /locus_tag="PLD53_03920"
     CDS             complement(9121..10050)
                     /gene="mreC"
                     /locus_tag="PLD53_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRC60084.1"
                     /translation="MDHQPPPFFKRGPAPLARLTFYAALSLALIFVDSRFQTLELLRQ
                     GLSLFTHPIQQAAHAPVEALQNVGSYFSAISRFQDENARLKRAQLDVAETRLRTQHLE
                     VENERLRKLLDVKSRLKANGRVTQILYAARDPFSRRVIADKGQQDNIIPGQPVVDDAG
                     VVGQVTRVFPFVSEITLITDKEQAVPVQVVRNGLRSVVFGLGNGQLELRFMPANADVQ
                     IGDLLVTSGLDGIWLPGLPVAKVVSIERDTSYSFARIYCVPVAGVENFGEVMVLDPRE
                     TIPLPAQLNSEAADIGAKSSAKSGAKKKRLQKE"
     gene            complement(10213..11256)
                     /locus_tag="PLD53_03925"
     CDS             complement(10213..11256)
                     /locus_tag="PLD53_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRC60085.1"
                     /translation="MFSFLRSYFSNDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRME
                     GGPNAKKTIQAVGLAAKNMLGKAPGAITVIRPMKDGVIADFTVTEQMLKQFIRKVHDS
                     RLLSPSPRIIICVPSGSTQVERRAIRESAIGAGASQVFLIEEPMAAAIGSDLPVSEPT
                     GSMVVDIGGGTTEVGVISLGGMVYAGSVRVGGDKLDEAIINYISRNYGMLIGENTAEN
                     IKKCIGSAFPGAEVREMEVSGINKAEGIPRKFTISSNEILEALTEPLNSIVSAVKSAL
                     EKTPPELGADIADKGMVLTGGGALLRDLDRLLMEETGLPVIVAEEPLTCVARGCGMAL
                     ERMDKLGSIFTSD"
BASE COUNT         2454 a         3192 c         3340 g         2308 t
ORIGIN      
        1 gaggtcaggc gcgcgtccca gacccaccag gtgccccaca tcggtttgcc ccacagcgcg
       61 cccgtccaca gcgagagaaa ggcgaatagc gcaccggtcg gcgccagtgc tgtggccatc
      121 atgcccgaca ggcggaaatt gaaggccagc cccagtccgg cccagaaagc catcaccagg
      181 taaatgaaca tcgacatcca cgacgccggt acgtgcagga agatgatgcg gtagccttcg
      241 ccctgctgcg cgtccgtagg cgctaccaga aagccgatgt agagcccggt gatgccgaaa
      301 atcgccgcca gcgtccagaa ccacggaatc attctgccgg caagcggata gaacgcttgc
      361 gggctggcaa atttgaacca gttgatcagt ttgttactca ttattccaag gcaatccgta
      421 atgccgctgc agttggccac ggtgcaaaaa acacgccgcc cagcgttagt gcgccaagca
      481 gcgacagatg ggcttgcgcc ccgagtccgg tgacgacagc atcaaccgcg cccgcgccaa
      541 aaataagcac cggaatatac aggggaagaa ccagcagcga aagtagcaca ccgctgccgc
      601 gcacgcccag cgtcagtgct gcaccaatgg ctccgatgcc ggacagcgcg ggggtgccga
      661 tcagcagcga gataacgagc gtccaaaggg cctcgctgga taggtcgaac tggattccga
      721 gcaccggtgc cagcagagtc agcggaagcc ccgaaaccag ccagtgtgca aacaccttgc
      781 cgagcacgat caagccgagt ggttgcggtg ccaacgcgag ctgttccagg gttccatcgc
      841 gatggtcgtc ggcaaacagg cgcggtagcg acaacagggt ggatagcagg gcggcgaccc
      901 acagtacacc cggtgccagc gtgcgcaaca gctccggctc cggcccgaca cccagcggaa
      961 acagactgac cacaatgatg aaaaagaaca gcgccgatac gatatccgac cggcgacgca
     1021 gggccagacg aaggtcgcga ccgacgatgg ctcgaatcag ggcaagcatt cgtcagtcgt
     1081 cactcaatgc gaagtagcga agcgggcgtc aacattttct atctcaatta gcattacttc
     1141 tagttcagcc gcagttcacg caccgtgcct gcgacaacct cgaccgcttg atgggtggtc
     1201 agcacggcca acccaccgcg ttgcagatgg gtaccgatca ggccggcaac cagttcgacc
     1261 gcggccgtat ccagcgccac gtagggttca tcgagcaccc acagcgccct gcgctcattg
     1321 accagcctgg ccagcgccac acggcgtttc tgcccctgtg agaggtagcg acaagccaga
     1381 tcctcacgcc cggcaaggcc gacaatctca agtgcatcaa gtgctgcaac ttcatcgaga
     1441 tgttcgccgg ctagtctggc ggaggacagc agattctcga gcggcgtcaa ctcttccttg
     1501 atggcgttga aatggccgag ataacacaac tcccggcgaa attcctcgcc gagcttgcca
     1561 atcagctcgc cccgccagcg aatctcgcca tcagccggcg gtgacagccc gcagagcatg
     1621 cgcagcagac ttgtcttgcc ggagccattt tttccctgta aatcaagcat ctcgccacca
     1681 tcaaggcgga agttgagctt cgaaaaaagg cttcgctcgc cgcgtacgca ttcaaggttg
     1741 gaggcttcta acatgcaggg aacccgaaag ggagcagagt cacattatgc agccttgaat
     1801 caggtttttg tttgatgcaa atcggactct caagtttgac tttgtcccgg agtagaatca
     1861 gcgtcaatac cccgagctat ttccctgaat ccacgttcaa tgctattgtg catgccggct
     1921 ttaacaaagt tgagtatgat gctatatctg ttaaggtgat ccggaacgat atgcgaagca
     1981 tacacgatca aggtttcctg tcaccggatc aaaaaagaga gaacgtgcct gaaaagggcc
     2041 gtctcgtcac tgaatggagt tgccgtgctt ttgccgtact tgttggcagc aagataacga
     2101 aaacataagg aaaatcaacc tgatccggca atccggcgta attccgggct gaaggaagtt
     2161 aacctttcaa cgaaaaagat acggaccctt acccgtgcaa acttccgata actcacacaa
     2221 cgtctgccag cgcatccggc tcagcggtgt cgtgcagggc gttggcttca ggccgtttgt
     2281 ctggcgtctg gccaaggagc ttctgcttac tggctgggtc agaaaagacg cgcgcggcgt
     2341 cgagattgaa gtgtgtggtg cagcggaaca ggtacagaat ctgatcgagc gcttgcagaa
     2401 cgatgctccg gtgaaggcgc acatcgattc ggtgacggcg cagaacagtc atgttgaatg
     2461 cgtagccgat gatttctata tcctgaatag tcgtagcggt cgtgctccag cgatggtcgg
     2521 acacgactcg gcagtctgcc gcgattgtct ggccgaaatg ttcgatccgg cgaaccggcg
     2581 ctggcgctac gcgttcacca actgcacaaa ttgcggtccg cgctacacaa ttgctcgtgc
     2641 ccttccgtac gctcgtgagc gaaccagcct gaagaacttt ccccgctgcc cgaagtgcca
     2701 aaacgaaacc caacgaaagg atgggcggca ccacaatgat gaggtgaatt gttgtccgaa
     2761 gtgtgggccg cagcctgaat tgctggatgc cgaggggcgg cctctgccgg gtgatccgat
     2821 tacgcaggcc gtggcgcttc tggcgcaggg gaagattgtc gcgatcaaag gcatgggcgg
     2881 gtttcatctg gcctgcgacg ctcgcaacgc ggttgcagtc gctgtccttc gcgagcgcaa
     2941 gcagcgggat gagaggcctt tccccgtgat gcttgccaac gcctcgtccg cgagtgcttt
     3001 tgttcaactg ggtatcggcg aaccgggtct gcttgcaatg gcagagcgac caatcattct
     3061 gctcaagaaa cgcccaagct gtgacaaagc cttgcccggt gtgacgaccg ggctgggctg
     3121 gttgggggtg atgatgccct tcacaccgct acagtacctg ctcttccatg aatctgctgg
     3181 tcgcccgcaa ggccttggct ggctggatcg tccgcagaaa ctcgcgctgg tcatgaccag
     3241 cgccgacccc ggtggtgagc caacagccat tagcaacgag gaagccttgc tgcgcctgtc
     3301 cggtattgcc gatgcttttc tgctgcatga ccgtgagata aagtcccgtt gcgacgacag
     3361 tattgcgcgc tcgggccctg gtgggctgca acttgtgcgc cgcgcgcgtg gctacgcgcc
     3421 gaacgcgatc aagctgccac gatccggccc gtctgttctg gcggtaggcg gttggttcaa
     3481 gaatacagtc tgcgtgaccc gtggcgacga agcgtttgtt tcgcagcata tcggcgatct
     3541 ggataacgcg gcagcctgtg attattttga atcaaccgta acgcgtctcc tcaagctcct
     3601 tggcatcaaa ccggcgctgg ttgcccatga tcttgaccgt agcttccatt cgacgcgatt
     3661 tgccctggac ttcgcacagc agaggggcgt tccggcgctc gccgttcaac atcaccacgc
     3721 tcacacggct gcggttcagg ccgaacaccg gattgacgct ccggttatcg ggctgtcgct
     3781 cgatggtatc ggcctggggg cggatggtgg cggttggggg ggagagctgt tgcgcgtcga
     3841 tggcgtatcg atggagcgta tcggtagtct tgcgccgatt gaactcgccg gtgagcaggc
     3901 gttgagagtg ccctggcgcc tggcggcggc ggtgctgcag caacttggtc gcggcaagga
     3961 aattgaaaag cgctttcccg agcagcggag cgctgcggcg gtcgaaaaaa tgcttctgca
     4021 aggcatcact tgtccaaaga catcgagcct tggctgttac atcaatgcgg cagcaggttt
     4081 gctcggcatc agctcgatga tggctttcga tggccaggcg gcggaattgc tcgaagggat
     4141 ggccgatcgt tacggtgatg ttctgccact caatggcgcc tggtcaattg aaaacggttg
     4201 tctgaatctg cagccgctgc tggccgttct ggccgatgaa aagatacccg aacgcggtgc
     4261 ggcgatcttt catgccacgc tggttgccgc cttgactgat tgggtgtgtg ccattgcgcc
     4321 gtcaggaagc aagatcgtgg gcagtggggg ctgtttcctc aaccaggtgc ttgcacgggg
     4381 tctgcgcgca cgtcttggag gccgtggcct gcaattgatc gaggctcgcc gtctgccgcc
     4441 caacgatgga ggactggccg tcggtcaggc ctgggttgcc ctgcagtacc tgctggggta
     4501 gggcgaatta tcgagctaca aacccgggtt tgtagccgag cgccagtcga attttctcgg
     4561 ccactttttc ggcatcggcg cgacttgcgt aagggccgag atgtactcgg tacataccgc
     4621 cgccggcatt gatccggatc ccttcctgca accagtccag ctcgcgcgat aaatgcgaac
     4681 gcagattttc ggcattgtcg gcgttggaga aagccccaag ctgcaggaag acgcctgttg
     4741 tggcgacagg cggtgcggaa gccgagacag gcagccgtgc tgacgctccg gatggtgggg
     4801 attggtcgga ggcttcgtcg ccatccgctc gggtcgccag tatttcaatc acgtcgcgtt
     4861 gagccgcagg gaccggtgac ttggcggtct gagccggcgg gttgatctgg gcgtaggtca
     4921 gttcggttga gtcgccgggt aaaacagtct ctacttcaac ctgcgtgctg ccatcgttga
     4981 tatagccgag gcggtgcgca gcagcatagg agaggtcgat gattcggtcg gcatggaaag
     5041 ggccgcgatc cgttacgcga acaaccacgg tcttgccatt gctcacacgc ctgacccgca
     5101 cataggacgg cactgccagc gtcgggtgtg cagcggtcat cgagaacatg tcgtagggtt
     5161 cgccgattga ggttttctgg ccgtgaaact tctttccgta ccaactggcg atgcctgact
     5221 gccggaagtg tttgaggctg gtgtccggta tatacgactt gccaagcacg ttgtacggcc
     5281 tgttggcaaa gcgatgcagg ggttcgctgc gcggctgggc gtccggaatg tcgtccagat
     5341 tgtccggaat gtcgtcaccg gggccgtcgt ccttgtaata cccgccgcca tgcttttgta
     5401 tcacgccagg cttgcgcgca gggcttgttg attggttgcc gggcgacggg ccatcggtgg
     5461 cgcgtgggat ctgactgcta caggcggaaa gaaacaccag caccagcagc agctcaggca
     5521 gtgcgttgcg cttcttgcct gtcatgcgca ctcttctatt cactactcgc gtcatttttt
     5581 gaccagcatt cggtgtgaat gaatgctcat gagaattccc aggccgagaa acaaggtcac
     5641 caacgcggtt ccaccatagc tcatgaacgg cagcgggacc ccgaccaccg gcaaaatacc
     5701 gctgaccatg cccatgttaa cgaaggcata tgtgaagaag ctcaaggtga ttgatccacc
     5761 cagaacacgc gcgaaaagcg tcgaagcgtt ggccgagatc atgagtccac gggctatcag
     5821 cagcaaatag agggataaaa gaatactgtt gcccagcaag ccgcgctctt ccgagaacac
     5881 ggcgaaaatg aagtcggtat gtcgttcggg gataaattcc aggcgagttt gggttccctc
     5941 aagccagcct ttgccgaacc tgccgccgga gcccatggca atcgtcgact ggatgatgtg
     6001 atatccggaa ccgagcggat cggttgtcgg gtcgatcaat gtgagtatcc gcttgcgctg
     6061 gtagccgtgc agcatggtcc aagcaaaggg cgcgacactg gctcccgcga cgatcatgcc
     6121 gatgatcgct ttccatggca gaccggcaaa gaaaataacg tagaaacccg aagcgccgac
     6181 gagaatcgct gttcccaggt cgggctgctt ggcgatcaga ccgaatggaa tgagcagcaa
     6241 caggccggca atcacaaaat gtcggaaacg caagccggat tcgtacttgt gaaagtacca
     6301 ggccagcatc agaggcatcg caatcttgag aatctcggcc ggttggatgc gtgtgaaacc
     6361 caggggtaac cagcgtctgg ccccgttgac cttgactccc accaggaaca ccagaatcag
     6421 aagaataacg cccacgatat agagtgggac agcaaagcgc atcagctttt gcgggggagt
     6481 ctgggcaagg gcccacatta ccgcgacacc gatcccgagg ttgagcaact ggccggttgc
     6541 acgattgttg gagtcgtagg tggcgctata gacggttgct atcccgaacg ccatcaggac
     6601 cagcgctatt gccagcaagg ggccgtcgat atttccggtg aggcgcaccc agaaccggtt
     6661 cagaatttgc tggaacaaag cctaattctc ctcttccggc gtgtcgtctt cctttgccgc
     6721 gcctttgggc agcttgccca gcaagtagta gtcgagcacc atgcgagcga tcggcgctgc
     6781 cgattgagaa ccaaagccgc cattttcgac cagtacagcg agcgctatct tggggtgatc
     6841 cgccggcgca aaggcaatga agagaccatg gtcatgtaat tccttcttca gtttgccggc
     6901 tctgtattcg gcgcctttca ggctgaaaag ctgcgccgtg cctgtcttgc cgcccgaaac
     6961 gtattccgca ccggcaaaag cgcgggcgcc cgttccttcc ttattgacgc caaccatcgc
     7021 cttcttgatt atttctatgt tctcgggctt ccaggcgagt gtgcgcagtg gttcaggctc
     7081 gatcaagacc ttttcgcccg tcttggtatc cgtaatgtac ctgacgaggt gcggtcggta
     7141 gaacacaccg tcgttggcta cgaccgcgat tgcttgcgcc agctggatcg gtgtgtaggc
     7201 gttgtagcct tgaccgatcc cgatcgagat cgtttcgccg gcataccatt tctgttgctc
     7261 ccggcttttg aagcgacgcc ttttccattc cggcgaaggc agcacaccct ccgactcgcc
     7321 ttcaatatcg atgccggtac ggctacccag acccaggctg cccatgaagc gcgcgatatt
     7381 gtcgatgccc aaatcgttgg ctagcatgta gtagtaggtg tcgcaggaat ggacaatcga
     7441 cttgtacata tcgaccgtgc catgcccccc cttcttgtca tcgcgaaaat gatgtccgcc
     7501 gaagttgtag aagccggggt cggagattgt ctgaccggcc gtgcgtttgc cgatttcaag
     7561 cgcggccagg gccatgaaag gcttgaatgt tgagcccggt gggtaggccc cgttcagtgc
     7621 ccgattgacc atggggcgat ccggcgagtt gttgagcaaa tcccattcat ccgaacgaat
     7681 gccatcgaca aaaagattcg gatcgaaagt aggtgtcgat acgagcgcga gaattccgcc
     7741 ggtcgacggc tcgatggcga ccagcgcacc acgacgatcg ccaaacgcct gctcggtgat
     7801 ttcctgcaat ttggcatcga gtgtcagagt caggttattc cctgaaacag gaggggtgcg
     7861 cgacagactg cgaatcgcgt gacctcctgc atcgatttcg acctcctcat aacccgtctc
     7921 gccgtgcaat tgaaactcgt acttctgctc caggccggtt tttcctatgt ggtctgtgcc
     7981 tctgtaattg gctgcctgct cgttcttctc gatgatttca agatcggtct tgttgatccg
     8041 attgatatag ccaatcgcgt gcgaggctct tgagccgagt gggtattgcc ggaacagtcg
     8101 cgcctttact tcgacgccag gaaaacggta gcgattggcc gcaaatcggg caacttcctc
     8161 gtcgctcaat cgcgtgcgaa tcggcaggct ctcaaaagac ctgctctcat caagcagctt
     8221 gcggaatcga cgccgatcct tggactcaat ttctaccagt ttgccgagat cctcgatcat
     8281 tgcatcgaga ttctcgattt tggacggcgt gatttcgaga gtgaacgccg tgtagttccg
     8341 tgccagaacg atgccattgc gatccatgat aaccccgcgg ttgggggcaa tcggcaccag
     8401 tgaaatacga ttgtcttcgg ctcgcgttgc gtaatagtcg tgctgaatca cttgcaagta
     8461 gaaaaaccgc gcgaacagaa tggcgaagcc cagcaccacc acgctcgccg caatcacgac
     8521 gcggaagcgg aaccgttcaa tatcgctatc gtgtgtgttc tgcggtgaat accgggtacg
     8581 caaggcaaat ttcctcaaat tggccggttg gcgtctcggt cgactggttg atactgtggc
     8641 agaagcagaa tgaaagtgag tggaagccac aacagggcgc ctacgaaggg tccggcaaag
     8701 aagctcaatc cgggaaggat cgcacccggc atggcgcgaa caccgaattg cacaagctgg
     8761 acaagcagca acagcgggag gatatgcaag gcctgctgac cgagcggaaa ccacaggatg
     8821 cgacgcgata gcgcggaggc ggcatagata gccagaacgt aagcaagtgc atgctgcccc
     8881 atcaggctgg aatcggcgac atccatggcc aatccgaaaa tgaagcctga tcccatgccg
     8941 acgtaacgtt gctcgcgaat gctccagaag atgagtagca aagccgccca gtccggcatc
     9001 catggccagg ccgaggtcgg cagaaagttt agcagcagcg cggccaggag cgtgatcagg
     9061 atgtaccagg gccgaaccgg ctgcagaatg cgggatgagg atgaagtggg ttgcatcgac
     9121 ctattccttt tgcaatcgtt tcttcttcgc cccggatttg gccgaagatt ttgcgccaat
     9181 gtcagcggct tcgctgttca actgagcggg tagaggaatc gtctctcgcg gatcaagcac
     9241 catgacttcg ccgaagttct caacgcccgc gacggggacg cagtagatgc gagcaaacga
     9301 gtatgaggta tcgcgttcga tgctcacaac tttggccacc gggagaccgg gcaaccatat
     9361 cccatccagc cctgaagtta ccagcaggtc gccgatctgt acatccgcat tggccggcat
     9421 gaaacgcaac tcaagctggc cgttgcccag cccgaaaacg accgaacgca gcccgttgcg
     9481 cacgacctga acgggtacag cctgctcctt gtcggtaatc agggtgatct ccgaaacaaa
     9541 gggaaagacc cgggtgacct ggccgacgac acccgcgtca tcgacgaccg gttgcccggg
     9601 aatgatattg tcctgttggc ccttgtcggc gatcacgcgg cgcgagaagg ggtcgcgtgc
     9661 tgcataaagg atctgagtca cccgtccatt cgccttgagc ctgctcttta catcaagcag
     9721 cttgcgcagg cgctcgtttt ccacttcgag atgctgcgtg cgcaatcgtg tctcggcgac
     9781 gtccagttgg gcgcgtttca agcgggcatt ttcatcctga aagcgcgaga tggccgaaaa
     9841 atagctgccg acattctgga gtgcttcgac aggagcatgt gcagcctgtt gtatggggtg
     9901 ggtgaacaat gacaagcctt gcctgagtag ctcaagcgtc tgaaaacggg aatcaacgaa
     9961 aatcagggct aacgaaagcg cagcatagaa tgtcagccgg gccagcggtg caggtccgcg
    10021 cttgaagaac ggcggcggtt gatgatccat tgcttcagca aaaggtacgg aatgaggaac
    10081 aggaggtcat tgcggcaatc aacgcgcggt gattactcaa gaagttgacg acacgaacaa
    10141 gctgaacagc ccgcacaagt gacggggaag agcaataccg ctcacgcttc cccttttgtt
    10201 cgcgaccgca tctcagtccg aggtaaatat actgccgagc ttgtccatcc tctcgagcgc
    10261 catgccgcaa ccgcgcgcca cacaggtcag cggttcctcg gcgacgatca ccggcaggcc
    10321 ggtttcttcc atcagcaggc ggtcaaggtc gcgcaacagc gcaccaccac ccgtcagcac
    10381 cattcctttg tcggcgatgt cggcgccgag ttcgggcggc gttttttcga gtgctgactt
    10441 gaccgctgaa acaatgctgt tgagcggctc ggtgagcgct tcaagaattt cgttcgagga
    10501 aatggtaaat ttgcgcggaa tgccctcggc cttattgatt ccggagactt ccatttcacg
    10561 tacttcggca cccgggaacg ccgagccgat gcacttcttg atgttctcag ccgtgttttc
    10621 gccgatcaac atgccgtagt tacgactgat gtagttgatg atcgcttcgt caagcttgtc
    10681 gccccctacg cgcaccgaac ccgcataaac cataccgccg agggagatta ccccgacttc
    10741 ggttgtcccg ccgccgatgt cgacgaccat cgagccggtt ggctccgaaa ccggaagatc
    10801 cgaaccaatc gcggccgcca ttggctcttc gataagaaat acctgactgg caccagcacc
    10861 gatcgccgat tcgcgaatgg cgcggcgctc aacctgcgtc gaacccgaag ggacgcagat
    10921 gatgatgcgg gggcttggcg aaagcaggcg ggaatcgtgc accttgcgga tgaattgctt
    10981 gagcatctgt tcggtgacgg tgaagtcggc gatcacgccg tctttcattg ggcgaataac
    11041 cgtgatggcg cctggcgctt tgccaagcat attcttggcg gcaaggccga cagcctggat
    11101 cgtcttcttg gcgttcgggc cgccttccat gcgaatggcg acaactgacg gctcgtcgag
    11161 gacgatgcct ttgccgcgaa cgtagatcag cgtattggca gtacctagat cgattgccag
    11221 atcgtttgag aaataactgc gtagaaaact gaacatgcct gaatatccgt aaaagctccg
    11281 caggggctgc ggtc
//
LOCUS       DAORPM010000050        11189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1296_length_11189_cov_9.170648, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000050 DAORPM010000000
VERSION     DAORPM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11189
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1296_length_11189_cov_9.170648"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1852)
                     /locus_tag="PLD53_03930"
     CDS             complement(245..1852)
                     /locus_tag="PLD53_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60086.1"
                     /translation="MSDVSPNMSTAEVVPDLQTGLRIIARLPLTNPQLAESELDRLLD
                     SLLQSPPAAGVYLNLLEQMRMSLYLVEEERARHYISKPLPLDDDEACFQRVIATWSKV
                     ARAYAHCARIDPSPESAEQSFRAAMILHRCIYYTGMVLVEHHRARRELPRGLWLDLHA
                     YFTSAEKKGVATLSIPDALDPQGRSTHCTAAFISLLLSELAGPYSLSIRDQNLTRRWA
                     NSWSPLVSLHAAVPGEPLPSFVIDLTQDLGLCPSASSMQTEQLRRLDTSRLSMQLKQV
                     RMQLGQNISPAQTGLGEDCTAGQCKRLLEHLATPWSQARAPRKFRRHATTGVSRVCLG
                     FEEIHYFISGKEFNQPDNNRLHSHQEFESLFAFPHMVEPGQQLQTRQEQLGFAADQWE
                     VVNQSATGFRLIRRIAGKKVVHGQLLAICPHDGVSFLLAHVSWLMQERGGGLIAGISA
                     LPGIPQAVAARPLGQDAAHNELYCRAFLLPAVPTIGAEQSLVIPQGWYRSGRIVEICT
                     DAARRVKLLHVLEDGPDFERVSFTTAS"
     gene            2073..2498
                     /locus_tag="PLD53_03935"
     CDS             2073..2498
                     /locus_tag="PLD53_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRC60087.1"
                     /translation="MARIQIELPEHFDFSTGITLYLSHMNYGGHLDNALLLTVVSEAR
                     ARFFKSLGYTELDVNGVGIVVSDAALQYRSEAFHGEVMLVQMAAADFSQYGCDLPWRM
                     TEQASGREVARGKTGIVFFDYAARKIAQVPQDFRARFEP"
     gene            complement(2556..4013)
                     /locus_tag="PLD53_03940"
     CDS             complement(2556..4013)
                     /locus_tag="PLD53_03940"
                     /inference="COORDINATES: protein motif:HMM:NF021824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRC60088.1"
                     /translation="MPDSNTSSPVAPFSPFAILRNLVRIAPLTVSPSASVRETLTKLD
                     AARTDAIVVVDEASHVPLGIVTLRDVLRRIAIEAGDLEAPIAVIMTGGLITLPANATA
                     HQASVIMVRRGVRHLVLTEPDGSYFNVVSQADLYAQTGARVSELVSAILASRDIATLA
                     TLASDVRSFATRLLAERVGAESICQRISALNDLLTLQVIELTAGCFDLPYVPWCWLVF
                     GSEGRLEQTLSTDQDNGLVFAATSEAEAAALRKVFLPFAQAVNGALDACGFPLCKGNI
                     MASNPAWCLSLHEWQLAFGEWMSKSQPEALLNSTIFFDFRPLYGQDSLAGELREWLLA
                     RAPLYPIFLRCMVENSMNWESPLNWWQGFRYDGNKKYPHTIDLKMHGSRPFVDAARIH
                     ALANGVPDTNTVERLRGAAGLIGMQTHETAALVDAFYHIQRLRLENQVAGKTSDEPNR
                     VNPDQLHELDRHILKESLKQARSLQQRLARDYALT"
     gene            complement(3994..6156)
                     /locus_tag="PLD53_03945"
     CDS             complement(3994..6156)
                     /locus_tag="PLD53_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRC60089.1"
                     /translation="MSPGRKLVFLTTGVCLFLVTLVAATAALLLVGGAVEGGSDTSAA
                     EGRVFGVVALTLVSCWLAWSVISVLHDRYLRAALRMAEEVDAVISSGTRLGEYGSTEL
                     RVLGRAVNRLAAARDLLAGDVAMRVQEAKASLEQERSRLAALMAELNQSVVVCNLDGR
                     VLLYNQRAQVDLSTGSENMGSELLGLGRSIYQLFDRSLISHALERLQQRQGGNTEGQS
                     AQFVTATRAGRLLLAHMSPVMSTESSGQDAVPAAAITGFVLILDDITATNERDARRDA
                     LIQSLTEGGRGPLGNIRAAAETLSEFTDIAPEQRDRFLGVIQDEARALSRHLESAAHA
                     FSDDLRVRWPLEEMLGAELVGAAVRRISERSGLAVKINSVDESIWLRIDSFGLLQALS
                     YLTTRLHDEYQIREVRLALSLGGRLAQLDLMWSGTSMNNETAMTWELESMNTGGEATP
                     LSVREVIDRCNGEIWFQRERVSHRAYYRILLPAADSPTEALRSSLLPRQKSRPEFYDF
                     DLFQWSTSAHELDDRPLAELAYTVFDTETTGLRPSEGDEIIQIGATRIVNRRLLHNEC
                     FDQLIDPRHKLSQESISIHGITPDLLVGQPTIDSVLPAFHAFCADTVLLAHNAAFDMR
                     FLQLKQNALNLSFDQPVLDTLLLSAALHPNQASHRLEAIAERLGVSVIGRHTALGDAM
                     VTGEIFLKMLPLLEGIGIHTLRQAREASERTYHARLKY"
     gene            complement(6157..6525)
                     /locus_tag="PLD53_03950"
     CDS             complement(6157..6525)
                     /locus_tag="PLD53_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC60090.1"
                     /translation="MIKKVLIADDEQNIVISLDFLLRREGFEVLVAADGEEALAKIRS
                     DRPDLVLLDVMMPKMNGFDVCQALRADPELASIRVLMLTAKGRDTEVTKGLGLGADAY
                     MTKPFSTKELVVKVRALLGM"
     gene            complement(6522..9317)
                     /locus_tag="PLD53_03955"
     CDS             complement(6522..9317)
                     /locus_tag="PLD53_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRC60091.1"
                     /translation="MLNGSMIFAASLIYLGLLFAVAWWGDKRADQGRSIIANPTIYAL
                     SMAVYCTTWTFYGSVGRAAVSGIGFLPIYLGPTLVMALGWFLLLKMIRTAKANRITSI
                     ADFISSRYGKSHLLGGLVTVIAVVGIIPYIALQLKAVSGSVILLLNYPEIVMPGRSAA
                     LPVFADMSFYIALILAAFTIVFGTRHLDATERHEGMVAAIALESGVKLVAFLAVGLYV
                     TYGIYDGFADIFEHFADNEKLRRLTAAIPSGHGYTGWFSLTLLAGLAILFLPRQFQIA
                     VVENVNESHLKRAAWLFPLYLLLINIFVLPIAFGGLLHFSGKAVDADTFVLTLPMAHR
                     QEGLTLLVFIGGLSAATGMVIVETIALSTMVCNDLVMPILLRLRGAELAETKDLSRLL
                     LGIRRGAILVILLLGYLYFRLAGEAYALVSIGLISFSAVAQFAPAMIGGMYWRGGTRG
                     GALAGLSAGFLVWGYTLLLPSFAKSGWLAAEFLTQGPFGLSLLRPQQLFGLSGLDEIP
                     HCLFWSFFANIGAYIAFSVVRPPSAAEASQATLFVDALKHTRPVGAALWRGRAEVADL
                     LQLVGRFLGPERAKSAFAAYARRHGGAAEDAPEADAELVHFAESLLAGAIGSASARVM
                     VASVAKEEPLSLSEVMNILDEASQLRSYSRQLERKSRELEAATRDLKGANERLQELDR
                     VKDDIMSSVTHELRTPLTSIRAFSELLRDDPKMHLADRERFLSLIVSETERLARLVNQ
                     TLDLAKIESGNADWRGSTLDLKEVIEQSVAATSQLFSAKGGRIELDLPDGLPLILADR
                     DRLIQVMINLLSNAVKFLPPESGWVRVSLRTRAEGLEVSVIDNGPGIELANQQIIFEK
                     FRQVGDSMTDKPAGTGLGLPISRRIVEHFGGRLWVESVPGEGATFRFFLPLAQPESLA
                     ERAPVQGDPSQFGVEKT"
     gene            complement(9327..9527)
                     /locus_tag="PLD53_03960"
     CDS             complement(9327..9527)
                     /locus_tag="PLD53_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60092.1"
                     /translation="MHIRRLAGERLVAAFLGGCLLFNYPVLSLFDLGTELFGIPLIFL
                     YIFAAWAGLIAVMAWFIERPIE"
     gene            complement(9782..10255)
                     /locus_tag="PLD53_03965"
     CDS             complement(9782..10255)
                     /locus_tag="PLD53_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60093.1"
                     /translation="MRKRLIDEVKPATSLESGNWLNLENLVEVEISSEDAAHPIESAL
                     TPGAESGWRAAVSGKQTIRLVFNQPQRLRKILLSFVERSISRTQEYVLRWSADGGQSF
                     HEIVRQQWNFNPQGANTETEEHQVELSGVNVLELVITPEIGGGDAVASLAHLRVA"
     gene            complement(10341..10715)
                     /locus_tag="PLD53_03970"
     CDS             complement(10341..10715)
                     /locus_tag="PLD53_03970"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRC60094.1"
                     /translation="MPEESPFKGKTGVQRVWNAFHYSLAGLRAAFTCEDAFRQEALLA
                     VLLIPASFFLPVSGIGRALMIASVLLVLIVELLNSAIEAAVDRVSLDRHHLSKRAKDI
                     GSAAVLLALINVVIVWGCVLLG"
     gene            complement(10831..>11189)
                     /locus_tag="PLD53_03975"
     CDS             complement(10831..>11189)
                     /locus_tag="PLD53_03975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRC60095.1"
                     /translation="AAADVFVFASRTETQGLVLLEAMAAGLPVIALAEMGTVDILNPG
                     RGAIAPEDNPYAFSVALTMLLRDTATRKRLSIEGRSYAAEWSDDKLSGKMADLYRQIH
                     SRHIKLLQHDLKPATY"
BASE COUNT         2554 a         3291 c         3193 g         2151 t
ORIGIN      
        1 ccattaagct caatgtatcc ataacgcact atttggtaaa ggtttgttaa gcccaattaa
       61 tgcgaaggcc tatacgggga atccgattcg taatgcgaag gtcggcagtt cgactccgcc
      121 cagcggcacc agtcatacca agcacttggc ccaacccgca aaggttgggc caagtgcttt
      181 ctggcaattg tgtcaacagt aagcgaatcg gctgcgggtc cagctcggag cttcgtacgt
      241 ggtgttatga ggcggtggtg aagctcaccc gctcaaaatc cggaccgtcc tcaagcacgt
      301 gcaatagctt cacgcgcctg gcagcatccg tacagatctc gacgatgcgg ccgctacgat
      361 accagccctg cgggatgacc agcgattgtt cggcgccaat cgtcggcacg gccggaagta
      421 aaaaggcgcg gcaatacaat tcattgtgtg ccgcatcctg gcccagcggg cgggccgcaa
      481 cggcctgcgg gataccgggc aatgccgaaa taccggcgat caatccaccc ccccgttcct
      541 gcatcaacca gcttacatgc gcaagcagga aactcacccc gtcgtgcgga cagatggcaa
      601 gcaactgccc gtgcacaact ttcttgccgg cgatgcgacg tatcaatcga aagcccgtgg
      661 ccgattggtt aacgacttcc cattggtcgg ccgcaaagcc gagctgttcc tgacgcgtct
      721 gcaactgctg cccaggttcg accatgtggg ggaaagcaaa cagactctcg aattcctgat
      781 gtgagtggag tcgattgttg tcaggctggt tgaattcttt tccggagatg aagtaatgaa
      841 tctcctcaaa accgaggcat accctggaaa cccctgtcgt ggcatggcgg cggaacttgc
      901 gcggagcgcg ggcctgagac cagggtgtgg cgaggtgctc aagcaagcgt ttgcattggc
      961 cagccgtaca gtcttcgccg aggccggtct gcgcgggtga gatattctga cccagctgca
     1021 tccggacctg cttcaactgc atgctcagcc gcgatgtatc cagacgacga agctgctccg
     1081 tctgcatgct gctggccgac ggacagagcc cgaggtcctg cgtcagatcg atgacaaacg
     1141 acggcaaggg ctcgcctgga acggcggcgt gcaagctgac cagcggcgac cagctattgg
     1201 cccagcgacg agtcaggttc tggtctcgga ttgagaggct gtaggggccc gcaagctcgg
     1261 agagcagcag actgataaag gctgctgtgc aatgcgtgct gcgcccctgc ggatcgagag
     1321 catcgggaat ggacaacgtg gccacgcctt ttttctcggc agaggtgaaa tacgcgtgca
     1381 aatccagcca caagccgcgc ggcaattcgc gccgagcgcg atggtgctcg accaatacca
     1441 tacccgtgta gtaaatgcag cgatgcagga tcattgccgc acgaaacgat tgttcggcgc
     1501 tttccgggga ggggtcgatc cgggcgcagt gagcgtaggc acgggctact ttggaccatg
     1561 tggcaataac ccgttgaaaa catgcctcat catcgtccag cggtagtggc ttgctgatgt
     1621 agtgtcgggc acgctcctcc tcgacgaggt agagcgacat ccgcatttgt tcgagcaaat
     1681 tcagatatac gccagccgct ggaggcgact gcagcaggct gtcgagcaag cggtcaagtt
     1741 cgctttccgc cagttgcgga ttggtcaggg gcaatcgagc gataatccgc agaccggttt
     1801 gcagatcagg gacgacctcg gcggtcgaca tgtttggact cacatcactc atgtcctgct
     1861 catccttcag aattggccat tgacaggcgc ttgctggcac ttgccacttc agtcgcgctt
     1921 acccatgtta atgacgatga tgtttcatca tacgtggcgt cccgccaaca ggcaatgacc
     1981 ccgattgccg cgcgcaggcg atctggcatg acgtgctgat atactgtgcg cgcgactgag
     2041 ttgcttccca accaataatt gcagagaaac ctatggcccg tatccagatc gaactccccg
     2101 aacattttga tttttccacg gggattacgc tctatctgag tcatatgaat tatggcggtc
     2161 acctggacaa cgcactgctg ctcaccgttg tttcggaggc tcgcgcgaga ttctttaaat
     2221 ctctcggcta taccgaactg gacgtcaacg gagttggcat tgttgtttcg gatgctgcgc
     2281 tccagtaccg ctccgaggct ttccacggcg aggtgatgct ggtgcaaatg gccgcagccg
     2341 atttcagtca gtatggctgc gacctgccct ggcgcatgac tgagcaggcg tctgggcgcg
     2401 aggtggcgcg cgggaaaacc gggattgtgt tctttgatta cgcagcgcgc aaaattgcac
     2461 aggtgccaca agatttccgc gcacgattcg agccttgagg gtgttgaatc tgtggtgacg
     2521 gaagaaaccg catagcgcct gtccggccac aggctctaag tcagcgcgta gtcccttgcc
     2581 agccgctgct gcaggctgcg tgcctgtttc agcgactcct tcaggatgtg tcggtcaagc
     2641 tcatgcaact gatcgggatt gacgcgattg ggttcatcgc tcgtttttcc ggcaacctga
     2701 ttctcgagcc gcaaacgctg aatatgatag aaggcgtcga ccagtgcagc cgtctcatgc
     2761 gtctgcatgc cgatcaaccc ggcagcgccg cgcagccgtt cgacggtgtt ggtatccggt
     2821 acgccattgg ccagcgcatg gatgcgcgcc gcatcgacga agggacggga accgtgcatc
     2881 ttcagatcta tggtgtgcgg atacttcttg ttgccgtcat aacggaaacc ctgccaccag
     2941 ttgagcggcg attcccagtt catggaattc tcgaccatac aacgcaggaa aatcggatag
     3001 agcggcgccc tggcaagcaa ccattcacgc agttcgccgg ccagcgaatc ctgcccgtaa
     3061 agtgggcgaa aatcgaagaa tatggtcgaa ttgagcagcg cctcgggctg actcttggac
     3121 atccactcac cgaacgccag ttgccactca tggagggata ggcaccaggc cggattgctg
     3181 gccatgatat tgcctttgca caagggaaag ccgcaagcat ccagcgcccc gttgaccgcc
     3241 tgagcaaaag gcaggaacac cttgcgcagg gcggcggctt cggcttccga agtcgcggca
     3301 aatacaaggc cgttgtcctg atcggttgat agtgtctgct ccagtcgacc ctccgaacca
     3361 aaaaccagcc agcaccaggg cacataaggc aaatcgaaac atccagcggt cagttcgatc
     3421 acctgaagcg tcagcaaatc attgagcgcc gaaatgcgct ggcaaatgga ttcggcgccg
     3481 acccgttctg ccagtagccg agtggcgaag ctgcgcacgt cggaggccaa ggtagccagt
     3541 gtggcgatat cacgcgatgc cagaattgcc gataccaact cggagacacg tgcgccggtc
     3601 tgcgcgtaca gatccgcctg agacacaaca ttgaaatagc tgccgtcagg ctcggtaagc
     3661 accagatgtc ggacgccgcg tcgcaccatg atgacactcg cctgatgcgc agtcgcgttg
     3721 gctggcaggg tgatcaggcc gccggtcatg atcacggcga ttggcgcttc gaggtcgccc
     3781 gcttctatag cgatgcggcg caaaacatcg cgcaatgtca caattccgag cggaacgtgg
     3841 ctggcctcgt cgaccacgac aatcgcgtcg gtgcgggccg catccagttt agtcagtgtt
     3901 tcgcgaacgc ttgctgacgg ggaaacggta agcggtgcga tgcgaaccag attgcgcaga
     3961 atggcaaagg gcgaaaacgg cgcaacaggc gagctagtat ttgagtcggg catgataagt
     4021 cctttcgctt gcttcgcgtg cctgacgcag ggtgtgaatg ccgattcctt caagtagcgg
     4081 caacattttc aggaatatct ccccggtcac catggcgtca cccagcgcag tgtggcgacc
     4141 gatgaccgaa acgcccagcc gttctgcaat ggcttcaagg cgatgtgaag cctgattggg
     4201 atgcagcgcg gcggagagca acagcgtgtc cagcaccggc tggtcaaaac tcagattcag
     4261 ggcattctgc ttcaactgca ggaagcgcat gtcgaaggcg gcattgtgtg cgagcagtac
     4321 ggtgtcggca cagaaggcgt ggaatgccgg cagcaccgaa tcgatggttg gctgtccgac
     4381 cagcagatcc ggagtaatgc cgtgtatgct gatcgactcc tgtgacagct tgtgccgggg
     4441 atcgatcagt tgatcgaagc attcgttatg caagagtcgg cgattgacaa ttcgcgtggc
     4501 accgatctgt atgatctcat cgccttccga gggccgaagc ccggtggttt ccgtatcgaa
     4561 aacggtatag gcaagttcgg ccagtggacg atcatcgagt tcatgtgctg aagtcgacca
     4621 ttggaagaga tcgaaatcgt aaaactccgg acggctcttc tgccgaggca gcagactgct
     4681 gcgcaacgct tccgtgggtg aatcggcggc cggaagcagg atgcgatagt aggcgcggtg
     4741 tgaaacacgc tcgcgctgga accagatctc gccgttacaa cgatcgatca cctcgcgcac
     4801 agacagggga gtcgcctcgc cgccggtgtt catcgattcg agctcccagg tcatcgctgt
     4861 ttcgttgttc attgatgtgc ccgaccacat caggtcgagc tgggccaagc gcccaccgag
     4921 ggataaggca agccgcacct cgcggatctg atactcgtcg tgcaggcgcg tagtcagata
     4981 ggacaaagcc tggagcaagc cgaagctgtc gatacgcagc cagatgctct catcgacgga
     5041 attgatcttg acggccagac cactgcgctc gctgatacga cggacggctg cgccgaccag
     5101 ctcggcacca agcatttctt ccagcggcca gcgcacacgc agatcgtccg agaaagcatg
     5161 cgccgccgat tcaagatgac gggatagcgc ccgcgcttcg tcctgaatga cacccagaaa
     5221 acgatcgcgc tgctccggcg cgatgtcggt gaattctgaa agcgtttcgg cggctgcccg
     5281 gatgttgccc aaaggaccgc gcccgccttc ggtcaacgac tgaatcagtg catcgcgccg
     5341 ggcatcgcgc tcgttggtgg cggtgatgtc atcaaggatc aacacgaaac cggtgatggc
     5401 ggcagcaggc acggcatcct ggccacttga ttcagtcgac atcaccggcg acatatgcgc
     5461 cagcagcagc cgccccgctc gcgtagcggt aacgaattgt gccgactgcc cctcggtgtt
     5521 accgccctgc ctttgctgca agcgctcaag tgcatgcgag atcaggctgc ggtcgaataa
     5581 ctggtagatc gagcggccca ggccaagcaa ctcgcttccc atgttttccg agccggtact
     5641 caaatccact tgcgccctct ggttatagag cagcacgcgt ccatcaagat tacagacgac
     5701 tacactctgg ttgagttcgg ccatgagtgc ggcaagacga ctgcgttcct gttcgagcga
     5761 ggccttcgcc tcctgcacgc gcatagcaac atcacctgcc agcagatccc gggcagcagc
     5821 caagcgattg accgcgcgcc cgagcacgcg cagttcggtt gaaccatact caccaaggcg
     5881 agtgccgctg ctgatcacgg catcaacttc ctcggccatg cgcagcgcgg cgcgcagata
     5941 gcgatcatgc agtacggata tcacggacca ggcaagccag caggaaacca gcgtgagcgc
     6001 tacgacgccg aagactcgtc cttcagcagc ggatgtatcg cttcctccct ccaccgcgcc
     6061 acccactagc agcaatgcgg cagttgccgc gaccagggta actagaaaaa ggcaaacacc
     6121 cgtggtcagg aataccagct tgcgtccggg gctcactcac atccccagca atgcgcgtac
     6181 cttgacaacc agctccttgg tcgaaaaggg cttcgtcatg taggcatcgg cccccagccc
     6241 caaacccttg gtgacctcgg tatcgcgccc cttggcagta agcataagca cacgaatcga
     6301 tgccagctcg gggtcggcgc gcaacgcctg acatacatcg aagccattca ttttcggcat
     6361 catcacgtcg agcagaacaa gatcgggccg atcgcttcgt atcttggcaa gtgcctcttc
     6421 gccgtcggcc gctaccagaa cttcaaaacc ttcacgtcgc agcagaaaat ccaaagagat
     6481 gacgatattc tgttcatcgt ctgcaatcag cactttcttt atcatgtctt ctccactcca
     6541 aactggctcg gatctccctg cacaggagcg cgctctgcca agctttcagg ctgtgcaaga
     6601 ggcaaaaaga aacgaaatgt agccccctcg cccggtacgc tttctaccca cagacgccca
     6661 ccgaaatgtt caacgattcg tcgactgatc ggcaaaccga ggccagtgcc ggctggctta
     6721 tcggtcatcg aatcgccaac ctgcctgaat ttttcgaata tgatctgctg gtttgcgagt
     6781 tcaatgcccg gaccgttgtc gatgacgctc acttcaagac cttccgccct ggtacgcaag
     6841 cttactctaa cccaaccgga ctctggcgga agaaacttta ctgcattcga caggagattg
     6901 atcatcacct gaatcaagcg gtcgcgatcg gccagaatga gcggcaaccc gtcgggcaga
     6961 tcaagctcga tacgtccgcc cttggcactg aaaagctggc tggtcgccgc caccgactgc
     7021 tcgatgacct ccttcagatc gagtgtactg ccacgccagt cggcgttgcc tgactcgatt
     7081 tttgccaagt ccagggtctg gttcaccaga cgggcaaggc gctccgtctc gctgacaatc
     7141 agggaaagaa atctctcgcg gtcggcaaga tgcatcttgg gatcgtcgcg caataactct
     7201 gaaaacgcac gaattgacgt caacggtgtg cgcagttcgt gggtaaccga cgacatgatg
     7261 tcgtccttga cgcggtcgag ctcctgtagc cgttcattgg cgcctttaag atcgcgcgtg
     7321 gctgcttcga gctcgcgcga cttgcgttcg agctggcggc tatagctgcg cagttgcgat
     7381 gcttcatcga ggatgttcat gacttcggaa aggcttagcg gctcctcctt ggccaccgaa
     7441 gcgaccatca cccgtgctga cgcgctgcca attgctccgg caagcaagga ctcggcaaaa
     7501 tgcaccagct cggcatcggc ttctggcgca tcttcggcag caccgccgtg tctccgcgca
     7561 tacgctgcaa aggccgattt ggcgcgctcc ggcccaagaa aacgacctac cagttgcagc
     7621 agatccgcca cttcggcgcg cccgcgccac agcgccgcac cgacgggccg cgtgtgcttc
     7681 aaggcatcga caaagagcgt ggcctggctg gcctcggccg cgctcggcgg gcgcacaacg
     7741 gaaaaggcga tataggcgcc gatgttggcg aaaaaactcc agaacaggca gtgcggtatt
     7801 tcatcgagcc ctgacaagcc aaagagctgc tgtgggcgta gcagactgag gccgaatggc
     7861 ccctgcgtca ggaattcggc ggccagccag cctgatttgg caaacgaagg cagcagcagg
     7921 gtataccccc agacgaggaa tccggcgctc aagccggcta gcgcgccacc gcgcgttcca
     7981 ccacgccagt acataccgcc gatcatggcc ggcgcaaact gggccaccgc cgagaagctg
     8041 atcagtccga tggaaaccag ggcgtaggct tcgccagcta gccggaagta gagataaccg
     8101 agcaacagga tgacgagaat ggcccctcga cgaataccaa gcaaaagacg tgataaatcc
     8161 ttcgtttcgg ccagctcagc accgcgcaga cggagcagaa tcggcatcac caaatcgtta
     8221 cagaccatgg tcgataacgc gatggtttcg acaatgacca tgccggtagc tgccgacagg
     8281 ccaccgatga aaaccagcag ggtcagtcct tcctgccggt gagccatggg caaggtcagg
     8341 acaaaggtat cggcatcgac agccttcccg gaaaaatgca acaggccccc gaaggctatc
     8401 ggcagcacaa agatattgat cagcagcaga tagagcggga acagccacgc cgcccgtttg
     8461 agatgcgatt cgttaacgtt ttcgaccaca gcgatctgaa actggcgcgg caggaacagg
     8521 atcgccagac cagccagcaa cgtcagcgaa aaccaacccg tatatccgtg ccctgaggga
     8581 atggcggcag tcagccgacg cagtttttca ttgtctgcga aatgctcgaa gatatcggcg
     8641 aaaccatcgt agattccata agtaacgtac aggccaactg cgaggaacgc caccagtttg
     8701 acgccggatt cgagcgcaat cgccgccacc atcccttcgt ggcgctcggt agcatcgagg
     8761 tggcgcgtgc cgaacacaat ggtgaatgcg gcaaggatca acgcaatata gaaactcatg
     8821 tcggcaaaga ccggcaatgc ggcactgcgg ccgggcatga cgatttccgg gtaattgagc
     8881 aacaggatga cgctgccgga aaccgctttg agctgtagag cgatgtacgg aatgatgccg
     8941 acaacggcga tcaccgtaac caagccgccg agcagatggc ttttcccata gcgcgaggaa
     9001 ataaagtcgg caatcgatgt aatgcgattg gccttcgccg tacggatcat cttgagcaac
     9061 aggaaccaac ccagtgccat gaccagcgtc gggccgagat agatcggcag aaagccgatt
     9121 cccgagacgg cggcgcggcc gacactaccg tagaaagtcc aggtcgtgca atagaccgcc
     9181 atcgataggg catagatggt cggattggcg atgatggatc gcccctgatc ggcgcgcttg
     9241 tctccccacc aggcgaccgc gaagagcagc cccagataga tcagcgaggc ggcaaagatc
     9301 atcgatccgt tgagcatcga cgcggcctac tcgatcgggc gttcgataaa ccaggccatg
     9361 acggcgatca acccggccca ggcagcaaaa atgtaaagga atatcagcgg aatgccgaac
     9421 agttcggtgc cgagatcaaa cagcgaaagc accggatagt tgaacagcaa gcagccccca
     9481 agaaaagcgg ctacaagtcg ctcgcccgcg agtctcctga tgtgcatgct cttcccccgc
     9541 cgtgaagttc ggtgacgcta gtttgcatca ctctacccca tttgcgccac tttcatgaat
     9601 gttcactcgc cgcgctcgac tgctactctc cggtgccgat tggcagaaca gtgcgcacca
     9661 aaggatagtg gcgctcaagc catcagcgag caatggatgt agctcatcac ggtgcgcgct
     9721 ttacggtagt aagttggccg gtggtggcgt gcctttgcgg gtcacgtccg ccacgcgcga
     9781 atcaggcgac tcgaaggtgt gccagcgatg cgacggcgtc gccgccacca atctccggcg
     9841 taatgaccag ctcgagaacg ttgacgccgg acagttcaac ctgatgctct tcggtctcgg
     9901 tgttggcgcc ttgcgggttg aagttccatt gttgacgcac gatttcatgg aatgactggc
     9961 ccccatcggc agaccaccgc aagacatact cctgcgtacg cgatatggat cgctcaacaa
    10021 acgaaagcag tatctttcgc agccgttgtg gctggttgaa gaccaggcgg atcgtttgtt
    10081 tgccagacac cgcagcacgc caaccagact ctgcaccggg cgtcaaagcg gattcaatgg
    10141 ggtgagcggc atcctccgag ctgatctcaa cctccaccag attctcgaga ttgagccagt
    10201 taccactctc caatgaggtc gctggcttga cctcgtcaat caatcgtttg cgcatctatc
    10261 catcctcaaa gaaatagaac ttgtcttagg gggatcatgc catagaaaca agcgcatggt
    10321 cgaacaaccg ggcgctactc tcatccaagc agtacgcaac cccagacaat aaccacgttg
    10381 atcaacgcca gaagaaccgc tgcactgccg atgtcctttg cgcgtttgga cagatgatgc
    10441 cggtcaagcg aaacgcgatc caccgcggct tcaatcgccg aattgagcaa ttcaacaatc
    10501 aacaccaaca agacgctggc gatcatcaga gcccgtccaa tcccgctcac gggtaaaaaa
    10561 aagcttgcgg gaatcagcag gacggcgagc aacgcttcct gacggaaagc atcttcgcac
    10621 gtaaaggccg cacgcaaacc ggcaagcgaa tagtgaaagg cattccaaac ccgctgcacg
    10681 ccggtctttc ccttgaaagg gctttcctca ggcacagact tctccttgcc agaaactcga
    10741 atctgcaagt atacaggctt tgccaagggt ttcctgacaa gaaggaaaca accgactcaa
    10801 cccacggtgc actgccaagc tggcgattct ctaataggtc gcaggcttga gatcgtgctg
    10861 gagcagcttg atatgtctgg aatgtatctg ccggtaaagg tccgccattt tcccggagag
    10921 cttatcgtca gaccactcgg ccgcgtagct acgcccctcg atcgagaggc gcttacgcgt
    10981 tgcggtgtcg cgcaacagca tagtcagtgc gaccgagaat gcatatggat tgtcttcagg
    11041 cgcgatcgcg ccgcggcctg ggttgaggat atccactgtc cccatttcag ccagggcaat
    11101 gaccgggaga ccggctgcca tcgcttccag aaggaccaaa ccctgtgttt cggtccgcga
    11161 agcaaagaca aaaacatccg ccgcggcgt
//
LOCUS       DAORPM010000051        11184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1298_length_11184_cov_11.934226, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000051 DAORPM010000000
VERSION     DAORPM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1298_length_11184_cov_11.934226"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /gene="dnaE"
                     /locus_tag="PLD53_03980"
     CDS             complement(<1..1936)
                     /gene="dnaE"
                     /locus_tag="PLD53_03980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRC60096.1"
                     /translation="MPESRFIHLRLHSEYSITDGIVRLDDAVARAVSYGMPALALTDL
                     ANFFGLVKFYSSARGMGVKPVLGADVCIANASDPDRPYRLLLLCRSRQGYLQLCELLT
                     QAYLAPRIRGRAEITHQMLREIGVDGLIALSGAGNGDIGEALLQGNREQAASRARDWA
                     SLFSDAFYLEVQRVHPRGQPQQESLIAATVHLASELELPLVATHPIQFLERDDFKAHE
                     ARVCIAEGYMLGDKRRPRLYTEEQYFKSPDEMTTLFSDLPEALENSVEIAKRCNLQIT
                     LGTNYLPEFPTPKGITLDDFLCQEAVLGLEKRLQQMFPDVAVREAQRPMYEARLNIET
                     ETIVQMGFPGYFLIVADFINWAKNNDVPVGPGRGSGAGSLVAFSLGITDLDPLRYALL
                     FERFLNPERVSMPDFDIDFCQDKRWRVIEYVREKYGAAAVSQIATFGTMSSKAVIRDV
                     GRVLDLPYNFCDQLSKMIPVEQNKPLSLAKAIEAEPQLKAKIEEGEEVRDLFDLAARL
                     EDLTRNVGMHAGGVLIAPGKLTDFCPLYSADGGGSVVSQFDKDDVEKIGLVKFDFLGL
                     RNLTIIKLAVDYVEKLTGARPDLDALTFDDPRAYQILKDANTTAIFQVESDGMKKLLK
                     KLAPDRFEDIIAVLALYRPGP"
     gene            2083..2571
                     /locus_tag="PLD53_03985"
     CDS             2083..2571
                     /locus_tag="PLD53_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HRC60097.1"
                     /translation="MYKKLRTTVLLAFCLLSAHSATADDAVWGALLGGGAGAAVGSSI
                     SGRNGAIVGGALGAATGAAIGSERDSRRGEYYAGFSPAPVYYAPPAYYPQPAYYPPQV
                     YYRQPVRIVRQPVYFVHGGHGRYYGGHHQNYGNHGYGHGKHSYGHGKHGNGHGSGDRH
                     GR"
     gene            complement(2629..3306)
                     /locus_tag="PLD53_03990"
     CDS             complement(2629..3306)
                     /locus_tag="PLD53_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRC60098.1"
                     /translation="MIPVVFADHGWLLARHSFSFADYFDPAEMGCGVLRVINEDRIAP
                     GKGFGEHGHRDMEIVTYVMAGALEHRDSLGNGEIIRPGEVQRMSAGRGIRHSEFNPSP
                     NEETHLLQIWIEPRESGIAPGYEQQILAPGHNVWRLIASPDASQGSALIHQDAWLYAA
                     QLEPGAALDYTLDPVRLAYVHLVRGHLTLNGVEIGSGDGVKISDESWLHFGATQESEA
                     LLFDLPP"
     gene            complement(3391..4014)
                     /locus_tag="PLD53_03995"
     CDS             complement(3391..4014)
                     /locus_tag="PLD53_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRC60099.1"
                     /translation="MKSFLIANPKGGSGKSTLATNLAGYFARCGHQVMLGDTDRQQSS
                     REWLAIRPDILPPIRSWDVAKDRPARPPKNTTHVVLDSAAGLHGKALERMLKQVRHVI
                     VPVQPSLFDIWATRHFLETLLEEKAVRKAQTFVAVVGMRVDARTRAATELERFLSSYD
                     VPVLTFLRDTQLYVQLAASGMTIFDLSPSRTEKDMAQWKPVMDWVDA"
     gene            complement(4011..5345)
                     /locus_tag="PLD53_04000"
     CDS             complement(4011..5345)
                     /locus_tag="PLD53_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRC60100.1"
                     /translation="MHKLRAKIVSIRDLLATAWPITLITAIGFVVAYQFVKPAPPRHM
                     TISTGGEAGAYYAYAKRYAALLAANGVTLEVRTSDGSAQNLKRLKDGEADVAFVQGGI
                     TEKLEDDDESDLRSLGSVSYEPLWVFYRNKRRIDKLDHLDGQRIAVGEDGSGIRGLAL
                     QLLEANEISIHSKKLVPVAGLNAAEALQQGEIDASFIISAQEAPVVQVLLRSPGIRVM
                     SFVQADAYTRRFGFLSKIVLPRGVVDLVRDTPPRDTVLLATTANVIVRNDLHPALVNL
                     LLQAMTEVNGKSGFFQPAGEFPAYKDQSFVLSDEAERYYKSGPPFLQRYLPFWIAVLV
                     ERLFVLALPIIVLLFPLLKIAPSIYNWRVRSKIFQCYGDLKFLENELREGYDSSRHAE
                     YLARLDRIEEDAYSRNIPLAFSDLLYTLREHINLVREKLFKLQAQPVTENKA"
     gene            complement(5345..6319)
                     /locus_tag="PLD53_04005"
     CDS             complement(5345..6319)
                     /locus_tag="PLD53_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRC60101.1"
                     /translation="MPFAIRIHQPGGPEVMRWEEVAVGDPAPGEARIRHTAVGLNYID
                     TYHRSGLYPLPMPAGLGMEGSGVVEAVGANVADLKPGDRVAYAGGPVGAYCQVRCLPA
                     DRLLKLPDEIDFRTGAAMMLQGLTSAYLLRRTYPVQPGDAVLIHAAAGGVGLIACQWA
                     KALGATVIGTVSNEAKAELASRHGCDHIIFYTREDVAKRVREITAGEGVAVVYDGVGK
                     DTFMGSLDSLRVRGMMVSFGNASGPVSPFDPLLLSQKGSLFITRPTLMHYIAKRDELL
                     ALGAELFAVVSSGKVKIEVNQTYALADAANAHRDLESRKTTGSTILLP"
     gene            complement(6544..>6897)
                     /locus_tag="PLD53_04010"
     CDS             complement(6544..>6897)
                     /locus_tag="PLD53_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HRC60102.1"
                     /translation="RLYLSYAQTRMLHGQTRYSLPSLFLEELPEDLLHKINRAPAYSN
                     NAATAPARAAVSPASGQTSANGLRIGQNVQHAKFGLGVIVSAEGRGTEARVQVNFGAH
                     GMKWLALEYAKLAPV"
     assembly_gap    6898..6997
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6998..8565)
                     /locus_tag="PLD53_04015"
     CDS             complement(<6998..8565)
                     /locus_tag="PLD53_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRC60103.1"
                     /translation="MPDILANLNPPQLAAVTLPPQHALILAGAGSGKTRVLTTRIAWL
                     ISTGQTGPHSILAVTFTNKAAKEMLARLAAMLPINIRGMWIGTFHGLCNRLLRAHHRD
                     AGLPALFQILDSADQLSAIKRLLKNFNVDDVKFPPRELAGFINAHKEQGIRAAQAEAY
                     DDYTRRRVELYAEYDSQCQREGVVDFAELLLRSYEMLQRNEPLRHHYQERFRHILVDE
                     FQDTNRLQYAWLKLLSGHNAPVSRPNGACVFAVGDDDQSIYAFRGAEVGNMRDLEREF
                     KVENVIRLEQNYRSQGNILDAANALIKNNRGRLGKNLWTDAGAGEPIRVFEGFSDIDE
                     ARFVVDEARELTLDGYTRNQIALLYRSNAQSRVLEHQLFTAGVPYRVYGGLRFFDRQE
                     IKHALAYLRLIGNPDDDTAFTRVVNFPTRGIGSRSIEALQEAAHRANSSLYNAAASLS
                     GKAGTAVGNFIRLIETLREETRALPLPEIIDHLIERSGLRQHYLAEKEGRERLENLDE
                     LINAATNFLHEESDT"
     gene            8803..9168
                     /locus_tag="PLD53_04020"
     CDS             8803..9168
                     /locus_tag="PLD53_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60104.1"
                     /translation="MKAPKILPWIARKNGISEQLALSLWRRAAGETEELTGDCDSSDY
                     YFLAVGRFLDLAEEEGEKCAERAPLGALSLVPRIGWLLRQQNRMLQLNLFAAKKSYIF
                     WLANWRALVFGQKPAVYKL"
     gene            complement(9171..10070)
                     /locus_tag="PLD53_04025"
     CDS             complement(9171..10070)
                     /locus_tag="PLD53_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRC60105.1"
                     /translation="MKHSTRFLIAGFLLVSTFSVAAASQANRAYALVDSVQMPAWVER
                     NGVRQPLEPGTALHDRDRLVTGADARVLIQLAEGSAVKLGANTQLDLNALGWRENRVF
                     AAVLDVAKGAFRFTTGLFSKRHQQRSVNVRIATITAGIRGTDVWGSADAERDLVCLLE
                     GRITVSHPQDDEVRELSEALSFYTVPKGKAPNPVGRVDKEQVIKWAAKTEVQPGSGYT
                     RRGGRWVVELAIFDGKNDALALFDRTRAAGYAAHIKPRPKQRGGYRYSVRVNQLATEP
                     EAEALAEKLKLLLELSAPVVTKQ"
     gene            10265..>11184
                     /gene="fbaA"
                     /locus_tag="PLD53_04030"
     CDS             10265..>11184
                     /gene="fbaA"
                     /locus_tag="PLD53_04030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRC60106.1"
                     /translation="MNAPQVKSGVATGDDLQALFALAKQKSFAMPAVNVINTSTVNAV
                     MEIAAEVKSPAIIQLSNGGAQFYAGKALKNEAQRACIAGAVSAAHHVHALAGMYGATV
                     VLHTDHAAKKLLPWIDGLLDAGEAYFKANGKPLFSSHMLDLSEEPLEENIEICSRYLQ
                     RMSKMGMTLEIELGVTGGEEDGVDNSDVDSSKLYTQPEDVAYAYEQLSKVSNRFTVAA
                     SFGNVHGVYKPGNVKLQPVILKNSQAFIEKKYGTKAQPIDFVFHGGSGSSLEEIREAI
                     SYGVIKMNIDTDTQWAFWEGIKNYYKKNEG"
BASE COUNT         2355 a         3160 c         3280 g         2289 t
ORIGIN      
        1 gcgggccggg acggtagagc gcgagtacgg cgatgatgtc ctcgaagcgg tcgggagcca
       61 gcttcttgag gagtttcttc atcccatcgg attcgacctg aaaaatcgcc gtggtgttgg
      121 cgtccttgag gatctggtag gcgcgggggt cgtcgaaggt gagtgcgtcg aggtcgggac
      181 gtgcaccggt gagcttttcg acgtaatcga ccgcaagctt gatgatggtc aagttgcgca
      241 gaccaagaaa gtcgaacttc accagtccga ttttctcgac atcgtccttg tcaaactgcg
      301 agaccaccga gccgccgcca tcggctgaat agagcgggca gaagtcggtg agcttgcccg
      361 gtgcgatgag cacgccacca gcgtgcatgc cgacgtttcg tgtcagatct tcgagtcgcg
      421 cagcgagatc aaaaaggtcg cgcacctcct cgccttcttc gatcttcgcc ttgagctgcg
      481 gctcggcttc gatggctttg gcaagcgaca agggtttgtt ctgttcgacc ggaatcatct
      541 tcgagagctg atcgcagaag ttgtacggca ggtcgagtac gcgcccgaca tctcgaatca
      601 ccgccttcga cgacatggtt ccaaaggtgg caatctgcga caccgctgcc gcgccatact
      661 tctcacgtac gtattcgatg acccgccagc gcttgtcctg gcagaagtcg atatcgaagt
      721 cgggcatcga tacccgttcc ggattcagaa agcgctcaaa tagcagtgcg tatcgcagcg
      781 gatcgagatc ggtgattccc agcgaaaagg cgacgagcga acccgcgccc gaaccgcgcc
      841 cggggccgac cggaacatcg ttgttctttg cccagttgat gaagtctgcg acgatcagga
      901 agtagcccgg aaaacccatc tgcacgatgg tttcagtctc gatattcagc cgcgcctcgt
      961 acatcgggcg ctgtgcctcg cgcacggcga catcggggaa catctgctgc aggcgttttt
     1021 caagaccaag cacggcctcc tgacacagga aatcgtcgag tgttatgcct ttcggagtcg
     1081 ggaactccgg cagatagttg gtgccgagtg tgatctgaag attgcagcgc ttggcaattt
     1141 cgaccgaatt ctcgagtgct tccggcagat cggaaaacag cgttgtcatt tcatccgggg
     1201 acttgaagta ttgctcctcg gtatacaacc tgggtcgtcg tttgtcgccg agcatgtagc
     1261 cttcggcgat gcagacgcgc gcttcgtgcg ccttgaaatc gtcgcgctca aggaactgga
     1321 tcggatgcgt ggccaccagc ggcaattcca gttcggatgc cagatgaacc gtcgccgcaa
     1381 tcaatgactc ctgttgcggc tgcccacgcg gatgtacgcg ctgcacttca agataaaagg
     1441 catcgctaaa caagcttgcc cagtcacgag cgcggctggc tgcctgttcc ctgttgcctt
     1501 gcagcagcgc ttcgccaata tcgccgttcc ccgcaccaga gagcgcaatc agtccatcca
     1561 ccccgatttc gcgaagcatc tggtgggtga tttcggcacg cccccggata cgcggcgcca
     1621 gataggcctg ggtcaacaac tcgcagagtt gcaggtaccc ctgccgcgat cggcaaagca
     1681 gcagcagtcg atagggtcga tcggggtctg acgcgttggc aatgcacaca tccgcgccga
     1741 gcactggctt gacccccatg ccacgcgccg acgaatagaa tttgaccagg ccgaagaagt
     1801 tggcgagatc cgtgagcgcc agcgccggca tgccataaga gaccgcccgt gctaccgcgt
     1861 cgtcaagacg aacgatgcca tcggtgatgg aatattcgct gtgcaggcgg aggtggatga
     1921 aacgtgattc gggcatccgg ccattctact ccgtagcaag gttcgcaccg agtagccgag
     1981 cgctgaaaat ccgagttcgt aagaatttgt aaagcgggtc agcgatacag atcacagtac
     2041 gttgatttga ctatcactct cacgacaagt aaaggatctc tcatgtacaa gaaactgcgc
     2101 actactgtgc tactcgcgtt ctgcctcctt tcggctcaca gcgcaaccgc cgatgatgcc
     2161 gtgtggggtg ctttactggg gggcggcgcg ggtgccgccg tgggtagctc gattagtggc
     2221 cgcaacggcg caatcgttgg tggtgcatta ggcgctgcca ccggcgcagc aatcggctcc
     2281 gagcgcgata gccgacgggg tgaatactac gcaggattct cgccagcccc ggtgtactac
     2341 gcgccgccgg catactatcc acagccggcg tattacccgc cgcaggtgta ctaccgccag
     2401 ccggtacgca tcgttcggca gccggtatat ttcgttcacg gggggcatgg tcggtactac
     2461 ggcggacatc accagaatta tggcaatcat ggctacggtc acggtaagca cagctatgga
     2521 catggcaagc acggcaacgg tcatggctcg ggtgatcgtc acgggcgttg agtcgcgtgc
     2581 cagcctgagc agcttgctgc gctgcgttcc gagaatattt ttaccctctc agggcggcaa
     2641 atcaaacagc agggcttcac tctcctgcgt ggcaccgaag tgcagccacg attcgtcgga
     2701 aatcttcacg ccgtcgccgg aaccgatctc cacgccgttc aaggtcaaat ggccgcgcac
     2761 caggtgcaca taggccagcc gtaccggatc gagcgtgtaa tccagcgcgg cgcccggttc
     2821 caattgcgca gcgtaaagcc aggcatcttg atggatcagt gcgctgcctt gcgaagcatc
     2881 gggtgaggcg atcagccgcc acacattgtg acccggcgcc agaatctgtt gctcgtaacc
     2941 tggcgcaata ccggattcac ggggctcgat ccagatctgc aacaagtgcg tctcttcatt
     3001 aggcgaaggg ttgaattcgc tgtggcggat gccgcgtccg gcgctcatgc gctgcacctc
     3061 tccggggcga atgatctcgc cattgcccag gctgtcgcga tgttcgagag cgccggccat
     3121 cacgtaggtt acgatctcca tgtcgcgatg cccgtgttca ccgaaacctt tgccaggtgc
     3181 gatacgatct tcgttgatca cacgcagcac accgcagccc atctcggccg gatcgaagta
     3241 gtctgcaaac gagaaactgt ggcgcgcgag cagccagcca tggtcggcaa aaaccacggg
     3301 aatcacttgg ccgaagttgc atcatgatcg actcctggcg aatgaatcaa acggtgctgc
     3361 cgttgaacga ttgctctcgc tgctaccgtc tcaagcgtct acccaatcca tgaccggttt
     3421 ccactgcgcc atgtccttct cggtgcgcga tggcgaaaga tcgaagatgg tcatgccgct
     3481 ggctgccagt tgcacataga gctgcgtatc ccgcagaaag gtgagaaccg gcacatcgta
     3541 ggaggagagg aagcgctcaa gctcggttgc tgctcgcgtc cgcgcatcga cgcgcatgcc
     3601 gacgactgcg acgaacgtct gtgccttgcg caccgccttt tcttcaagca gggtttcgag
     3661 aaaatggcgt gttgcccaaa tgtcgaaaag agaaggttga acgggaacga tgacatgcct
     3721 gacttgcttg agcattcgtt ccagcgcttt gccgtgcagt ccggcagcgg agtccaatac
     3781 gacgtgagtc gtattcttgg gtggccgtgc cggccggtct tttgcaacat cccagcttcg
     3841 tatcggaggc aggatatccg gacggatggc gagccattct cgcgacgatt gctgccggtc
     3901 ggtatcaccc agcatgacct gatggccaca acgggcaaaa tagccggcca ggttggtggc
     3961 cagtgtcgat tttcccgaac cgccctttgg gttagcaatc agaaaggatt tcacgccttg
     4021 ttttcggtca caggctgggc ctggagttta aatagttttt cgcgcacgag gttgatatgt
     4081 tcgcgcaggg tatacaacag atcgctgaag gcaagcggga tgttgcgcga ataggcgtcc
     4141 tcctcgatac gatcaagccg ggccagatac tccgcatggc gggaagaatc gtagccttca
     4201 cgcaattcgt tttccaagaa cttgagatcg ccatagcact ggaatatctt ggagcgtacc
     4261 cgccagttgt agattgaggg cgcgatcttg agcaagggga acagcaggac gatgatcggc
     4321 agcgccagca caaacaggcg ttcgaccagc accgcaatcc agaagggcag gtagcgctgc
     4381 aggaaaggag gcccggattt gtagtagcgt tcggcttcgt ccgaaagaac gaagctctga
     4441 tccttgtaag cgggaaattc accggcgggc tgaaaaaagc cgcttttgcc gttcacctcg
     4501 gtcatcgcct ggagcaggag gttgacgaga gccgggtgaa gatcatttcg cacgatcacg
     4561 ttcgcggttg tggccagaag taccgtgtct cttggtggcg tatcgcgtac caggtcaacc
     4621 acgccacgcg gcaaaactat ttttgagagg aaaccgaaac gcctcgtgta ggcatcggcc
     4681 tgaacgaagc tcatcacccg aattcccggt gaacgcagca gaacctgcac cacaggcgct
     4741 tcctgtgcgg agatgatgaa agacgcatcg atttcgccct gctgcagggc ttcggccgca
     4801 ttgagtcctg ccacgggtac gagtttcttg ctgtgtattg agatctcgtt ggcttcgagc
     4861 agttgcagag ccagcccgcg gataccgctt ccgtcttcac ctacggcgat gcgctggccg
     4921 tcgagatggt ccagtttgtc gatccggcgc ttgttgcgat agaagaccca cagcggctcg
     4981 taggacacgc tgcccagcga gcgcagatcg ctttcatcat cgtcttcgag tttctccgtt
     5041 attccccctt gtacaaatgc aacgtcggcc tctccatcct tcaaacgctt caggttctgc
     5101 gccgaaccgt ccgaagtccg gacctccagc gtgacgccgt ttgccgcaag aagtgcagcg
     5161 tagcgcttgg cataggcata gtaagcaccc gcctcgcctc cagtcgagat ggtcatgtgt
     5221 cgtggaggcg ccggcttgac aaactgatag gcgacaacga aacctatggc cgtgatcagg
     5281 gtgatcggcc aggctgtcgc cagcaggtcg cgaattgaaa cgatcttggc gcgaagtttg
     5341 tgcatcaagg cagaagtatc gtcgagccgg ttgttttgcg tgattcaagg tcgcgatgcg
     5401 cgttggcggc atcggccaac gcgtaggtct ggttgacctc gattttgacc ttgcccgagc
     5461 tgacgactgc aaagagttcg gctcccagcg caagcagttc atcgcgcttg gcgatgtagt
     5521 gcatcagggt cggccgggta atgaacagcg agcccttttg tgacagcaac agcgggtcaa
     5581 acggcgatac cgggccggag gcattgccaa aactgaccat catgccgcga acgcgcagac
     5641 tgtcgagcga gcccatgaag gtatccttgc cgacaccgtc atagacgacg gcgacgcctt
     5701 cgccagcggt gatttcacgt acgcgtttgg ccacgtcttc gcgggtgtag aagatgatgt
     5761 gatcgcagcc atgtcgactc gccagctcgg ccttggcctc gttggagacg gtgccgatga
     5821 ccgtcgcccc aagtgccttg gcccactggc aggcgatcag cccaaccccg ccagccgccg
     5881 cgtggatcag aacggcatcg ccgggctgaa ccgggtaggt acggcgcagc agataggcgc
     5941 tggtcagtcc ctgcaacatc atcgcggcgc cggtacggaa atcaatctca tccggcagtt
     6001 tcagcagccg atccgccggc aggcaacgta cttggcaata ggcgcccacc ggtccgccgg
     6061 cgtacgctac ccggtcaccg ggtttgagat cggcgacgtt tgccccgacc gcttccacga
     6121 ccccagaccc ctccatgccc aatccggcag gcatcgggag aggataaaga ccgctgcgat
     6181 gatacgtgtc gatatagttc aggccgactg ccgtatggcg gatgcgcgcc tcgccgggtg
     6241 ccgggtcgcc aacggcgact tcctcccaac gcatgacttc gggaccaccg ggctgatgaa
     6301 ttcgaatggc gaagggcatg atgttctcct gagtagacgt tgtgtcgtta ggtcatgagc
     6361 ttggtgtcgg ttcattcggc cgaagcatgg gctgaatgaa gacaggttct agcctagccg
     6421 gtgcgggcgg ttcgggcaag gcggaaatgg tgccaaactc atgccggctt ggagcagagt
     6481 tgaaccgagc gaaacgattc gtggaggcgt ctgccaacct ccgggaaata cacttgcgcg
     6541 gtctcagacc ggagccagtt tggcatactc cagcgccagc catttcatgc cgtgagcgcc
     6601 gaaattgact tgcacgcgcg cctccgtccc acgcccctcc gccgaaacaa ttacacccag
     6661 accgaacttg gcgtgctgca cgttttgccc gatgcgcagg ccgttcgccg aggtttgccc
     6721 ggacgctggc gacacagctg cacgtgcggg tgctgtcgcc gcgttgttgc tgtaggccgg
     6781 cgctcgattg atcttgtgca acaagtcctc gggcaactct tcgagaaaga gggagggcag
     6841 gctgtagcgg gtctggccat gcagcatgcg ggtttgcgcg tacgagaggt agagccgnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg tcggactctt cgtgcaggaa
     7021 gttggtcgca gcgttgatca gttcgtcgag gttctcaaga cgctcacgac cttccttctc
     7081 tgccaggtag tgctgtcgca ggccgcttct ctcgatcagg tggtcgatga tttccggcag
     7141 cggcagggcc cgggtttcct cgcgtagcgt ttcgatgagc cggatgaagt tgccgaccgc
     7201 cgtgcccgcc ttgccactca gactggccgc ggcgttgtac aggctggagt tggcgcggtg
     7261 cgccgcttcc tgcagggctt cgatgctgcg gctgccaatg cctcgcgtcg gaaaattgac
     7321 cacgcgggta aaggccgtgt cgtcgtccgg attgccgatc aggcgcagat aggccagtgc
     7381 atgcttgatt tcctggcgat caaagaagcg caacccacca taaacgcgat acgggacccc
     7441 ggctgtaaac agctgatgct cgaggacgcg tgactgggca ttggaacgat acaacaacgc
     7501 gatctggttg cgcgtatagc cgtcaagtgt cagttcgcgc gcttcatcga cgacaaagcg
     7561 tgcctcgtcg atgtccgaaa agccctcaaa tacccggatc ggctcgccgg caccggcgtc
     7621 ggtccataga ttcttgccga ggcggccccg attattcttg atcagtgcat tggccgcgtc
     7681 gaggatattg ccctgcgagc ggtagttctg ttcgaggcgg atcacatttt cgaccttgaa
     7741 ttcccgctcc aggtcgcgca tgttgccgac ttcggcgccg cgaaaagcgt aaatcgactg
     7801 gtcgtcatca ccgacggcaa aaacgcaggc gccgttgggg cgagaaactg gcgcgttgtg
     7861 cccagacagc agcttcagcc aggcatattg caggcggttg gtgtcctgaa actcatcgac
     7921 gagaatatgt cggaagcgct cctgatagtg gtgacgcaag ggctcgttgc gctgcagcat
     7981 ttcgtaggag cgcaacagca gttcggcaaa gtcgaccacc ccttcgcgct ggcactgcga
     8041 atcgtattcg gcataaagct caacgcggcg ccgggtgtag tcatcataag cctcagcctg
     8101 cgcggcgcgt atgccttgct ccttgtgtgc attgataaaa cccgccagtt ctcgcggagg
     8161 gaacttgaca tcatccacat tgaagttctt caacaggcgc ttgatagccg agagctgatc
     8221 ggccgagtcg agaatctgga acagcgcagg caaaccggca tcacggtgat gtgctcgcaa
     8281 caacctgttg cacagcccgt gaaaagtgcc gatccacatg ccgcgtatat tgatcggcag
     8341 catcgcggcc aggcgcgcca gcatttcctt cgccgccttg ttggtgaaag tcacggcgag
     8401 aatactgtgc ggcccggtct gtccggttga tataagccag gcaatacgcg tggtgagcac
     8461 acgcgttttc ccgcttccgg ctccggccag aatgagtgca tgctggggtg gcagggttac
     8521 cgcggcaagt tgcggtggat tgagattggc gaggatgtcg ggcatcaaac agggttttcg
     8581 gggaaagccg tatgattata gcggtcgcaa cagcaactga atgttgcaca tgcgcaaagc
     8641 agcggaatga aataggaacc aaaccgacaa atagttcacg ttgactgcgc agagttggat
     8701 tataatgttg ttgcactgca tcaattgaag acccacaagg tcgcggttcg atcaatattc
     8761 ctcagtgcct ggttaccgcc agaacacaag gagaaacaca gcatgaaagc accaaaaata
     8821 ctaccgtgga tagcccgcaa gaatggtatc agcgagcagc tggcgctaag tctatggcgc
     8881 cgagccgccg gggaaaccga agagctgacg ggtgattgcg acagttcgga ctactacttt
     8941 ctggccgtcg gtcgttttct tgatcttgcc gaagaagaag gcgagaagtg tgcagagcgc
     9001 gctccgttgg gcgccttgtc gctggtcccg cgcatcggct ggctgttgcg tcagcaaaac
     9061 cggatgctgc aactgaattt gttcgcagca aagaaatcct atattttttg gctggccaac
     9121 tggcgcgctc tagtcttcgg acagaagcca gcggtctaca aactctgaaa tcattgcttg
     9181 gtaacgacgg gagcgctcag ctcaagcaac agtttgagtt tttcggcgag tgcttcggct
     9241 tccggttcag tagctagctg attgacacga accgagtaac ggtaaccacc cctttgttta
     9301 ggacgcggtt tgatgtgagc ggcgtacccc gcggcacggg tgcggtcgaa gagagccaga
     9361 gcgtcgttct taccatcgaa aatcgccaat tcaaccaccc accgtccgcc gcgccgcgtg
     9421 tagccgctac ccggttgaac ctcggttttt gccgcccact tgatgacctg ttccttatcg
     9481 accctgccta ccggattggg cgcctttccc ttgggtacgg tgtagaaact aagcgcttcg
     9541 ctcagttcgc gcacctcatc atcctgcgga tgtgaaacgg taatccgtcc ttcgagcagg
     9601 cagacgaggt cgcgctcggc atccgcactg ccccaaacat cggtgccgcg aatgcctgcg
     9661 gtgatggtcg caatgcgtac gttgacggag cgttgctggt gccgcttgga gaataggccg
     9721 gtcgtaaaac ggaaggctcc cttggcaaca tccagcacgg cagcaaacac ccggttctcg
     9781 cgccagccga gagcattcag atcgagttga gtgttcgcgc ccagcttgac ggcacttcct
     9841 tcggcgagct ggatgagcac gcgtgcatcg gcgccggtca ccaggcgatc acggtcgtgc
     9901 agtgccgttc caggttcaag cggttgccgg actccatttc gctcgaccca ggcgggcatc
     9961 tgcacgctat caacgagagc ataggcgcgg ttagcttgcg aggcagcggc aaccgagaac
    10021 gtgctgacca acaaaaagcc ggcaatcagg aaacgagttg agtgtttcat cgtggaacca
    10081 gctttcacag gttggagata acgaatagtc tgattctatc aggctgacac aacgggtgga
    10141 tacgagctaa catcactgtt ctgaaagcgt ttttcttgtg tctagagata gagtcattga
    10201 cctgatgtac gaatacgagt aaagtttttt tccttccacc aaccgtcttt ttggatatct
    10261 catcatgaat gcaccgcagg taaaatcggg agttgccact ggcgatgatt tgcaagccct
    10321 ctttgcgctg gcaaaacaaa agagtttcgc catgccggcc gtcaatgtta tcaacacgag
    10381 tacagtgaac gccgtcatgg aaatcgctgc cgaagtcaaa tctccggcca ttatccagct
    10441 ctccaatggt ggcgcccagt tctacgctgg caaggccctg aaaaacgagg cgcaacgcgc
    10501 ctgtatcgct ggtgccgttt ccgcagcaca ccacgtgcac gccctggcgg gaatgtatgg
    10561 cgccacagtc gtgctgcata ccgaccacgc ggccaagaaa ctgctgccct ggatcgacgg
    10621 actgcttgac gctggcgaag cttacttcaa ggccaacggc aagccgctgt tcagctcgca
    10681 catgctcgat ctgtcggaag agccactgga agagaatatt gaaatctgct cgcgttatct
    10741 ccagcgcatg agcaagatgg gcatgacact ggaaattgag ctcggcgtca ctggcggtga
    10801 agaagacggt gtcgacaaca gcgatgtcga tagctccaag ctctacaccc agcctgaaga
    10861 tgtggcctac gcctacgagc aactgtccaa ggtcagcaac cgctttaccg tcgccgcttc
    10921 cttcggcaac gtgcatggcg tctacaaacc gggcaacgtc aaactgcagc cggtcattct
    10981 gaagaactcg caagccttca tcgagaagaa gtacggcacc aaggcgcaac cgatcgactt
    11041 cgtcttccac ggtggttcgg gatcgagtct tgaggaaatc cgcgaagcca tctcctacgg
    11101 cgtcatcaag atgaacattg acaccgatac gcaatgggcc ttctgggaag gcatcaagaa
    11161 ctactacaag aagaacgaag gcta
//
LOCUS       DAORPM010000052        11142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1309_length_11142_cov_11.941643, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000052 DAORPM010000000
VERSION     DAORPM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1309_length_11142_cov_11.941643"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2360
                     /gene="hrpA"
                     /locus_tag="PLD53_04035"
     CDS             <1..>2360
                     /gene="hrpA"
                     /locus_tag="PLD53_04035"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HRC60107.1"
                     /translation="KRESDYADCLAVDQRGLRSMARNMRHAFGKKRDDLQTEHDRLLE
                     KSRAAVLVRRVSLPKPEFADDLPINARRGEIASLIEKHPVVIVCGETGSGKTTQIPKI
                     CLELGRGITGLIGHTQPRRIAARATAARIAQELNSELGALVGYKIRFSDRTSKDAYVK
                     LMTDGILLAETQGDPWLGQYDTLILDEAHERSLNIDFLLGYIKQLLPRRPDLKLIVTS
                     ATIDAERFSKHFNNAPVIEVSGRMYPVEIRYRPVQNEEAEKEGERDLYDAIVDACDEL
                     SRGGGIDHGGDVLVFLPGEREIREAAEALRKHHPAPTEILPLFARLSAEEQSRIFKPH
                     TGRRIVLATNVAETSLTVPGIRFVVDTGLARIKRYSYRNKVEQLQIEKIAQASANQRS
                     GRCGRVAAGVCIRLYDEQEFGLRPPYADPEILRSSLAGVILRMKALRIGDVESFPFIE
                     APPPKAISDGYQLLLELGAVADDRALTPIGQELAKMPLDPRVARMIVAAREEGCLREM
                     LVIAAALTVQDPRERPQERQGSADAAHQQFADQKSEFLSWLKLWNWYEGAVEHKKSQR
                     KLVQACHENFLSALRMREWRDIHKQLHGLAAEHDWKENQIPATYEAIHRALLAGLLGN
                     IGCKSEDSAHYLGARGMKFLIHPGSALNKKAGKWIAAAEITETTKLFARCVARIEPQW
                     LETVGAHLIKRGWFDPHWEKKAMQVVAFERSTLYGIVVNPKKRVNFGPLNPQEAREIF
                     IRQGLVAGEIAEEYARRWKFFTHNHQLMLDIETLEHKSRRPDVLVD"
     assembly_gap    2361..2460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2461..3759
                     /locus_tag="PLD53_04040"
     CDS             <2461..3759
                     /locus_tag="PLD53_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HRC60108.1"
                     /translation="TVPLAQLNQIPAVQCEWLVPGLLKEKVIQLVKTLPQKIRSKLVP
                     VPEFAAEFVVAVPPSGKSLVSSLIHYILQSRGLNARGWEITPDAFRPDALPAHFSFNF
                     KLVDEHDRQLGMSRSLSDLRGEWGGKAKQEFSELHETPSEYSGMTDWSFGELAEVMEV
                     EMAGGGSGQTVLGYPGLSDDGDSVSLCVFDSPEEAREAHATGLLRLFMLQFPDQLKYF
                     EKNLPGLTQMAMVYMPLGSLDDLRRQLIELTFVRACMAEPWPTDAENFRRRCAEAKTR
                     LGLLAQEICRLVGQILADWQSLQKRLTAFRSHAVALQDVEKQLGRLIGKRFVAETPFE
                     RLQHYPRYLMAITRRLEKLKAGGAQGELRDSRLMAEYAPLWTNYERRAIVLAKQGVSD
                     PQVEQFRWMLEELRVQLFAQELRTPAPVSSKRLQKMWEGL"
     gene            3779..4522
                     /locus_tag="PLD53_04045"
     CDS             3779..4522
                     /locus_tag="PLD53_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HRC60109.1"
                     /translation="MPEVILALRRSLVTLGQGRVWLYFFGPAIVALLIMIGLSVFLLD
                     YLIASLVEQPPMSWIASWGAFWLAKLLAALGGWLLILSASYLIAMLLTAVVVLPLLLN
                     RLSTTDYPELARMGSDSVAAATWNSIWAAVLFIIGWLVTLPLWLVPGLGLILPFFWMA
                     WLNRRTFAYDSLAAHATNDEWCELRRRQSMPLLALGLIMALLAHVPFMGLLAPSLAAL
                     AYVHFCLEALRRLRQGAVVSIIEKLEVSA"
     gene            4519..5274
                     /locus_tag="PLD53_04050"
     CDS             4519..5274
                     /locus_tag="PLD53_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRC60110.1"
                     /translation="MKFGAIIIGDEIISGKRIDQHFARIIALLGARGLRLSWAEYLGD
                     ERPRIAETLKRSFAAGDVVFSFGGIGNTPDDHTRQAAAEALGVDLVLHPDAEREIRAR
                     FGGEVNAVRLLLGTFPRGVDIVPNPFNRIAGFCVRDHYFLPGFPQMAHPMAEWALDTF
                     YPHLFNRHPMVDRALLLTGPNAYESALLDLMERIVADYPALRLFSLPSVGENGQRRHL
                     ELGVEGEASMVDQAMEEIRQEVDRRNIAWSWRE"
     gene            complement(5418..5975)
                     /locus_tag="PLD53_04055"
     CDS             complement(5418..5975)
                     /locus_tag="PLD53_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRC60111.1"
                     /translation="MITTPEQLVAANKASVDALLSLANTALASAERVAALNLNTARSM
                     MEDGMNNTKAMMGAKDPREALAMTAAQIQPAVEQIVAYNRSLYEITAQSKEEVTKQLE
                     SQFSDFQKQVATLMEKAAKNAPAGSDVAVAAVKSALEAASSAFDNMKNVVKQAAEIAE
                     ANIATASNATVNATVNAVNSAAKKK"
     gene            complement(6233..6832)
                     /gene="lptA"
                     /locus_tag="PLD53_04060"
     CDS             complement(6233..6832)
                     /gene="lptA"
                     /locus_tag="PLD53_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HRC60112.1"
                     /translation="MKPNSPYLAATFLLLALSLPALAEKADREKPINLEADRISMDDV
                     NKVQVFEGNVILTQGTLQIRTSKLVVTQDADGFQKGVATGGADGLARFRQKREGSSDY
                     IEGEAERIVHHARDEKSEFFIRAWVKSGLDEIRGQYISYDALTEKYLVTNGGGESKSA
                     TGTAQARVRAIIQPKGKNAAEEPKGEPLTLKPSGTVAPR"
     gene            complement(6829..7416)
                     /gene="lptC"
                     /locus_tag="PLD53_04065"
     CDS             complement(6829..7416)
                     /gene="lptC"
                     /locus_tag="PLD53_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRC60113.1"
                     /translation="MKNWGSAIFPLSILLVLTGLSFWLRYATELDGPRHDGKNRHDPD
                     YIISDSTLHKLDQNGRLQYTLKAADIRHYPDDDSTDLIKPDLVYLHSKKPPLSLSAER
                     GHVSRDGEQVDLYDNVRVHREASAKDEAMTAYMPQLTVLPDDERAFTKSPVLITRGKS
                     WLKGVGMQVNNRTQMYEIESQAVAVLESKHAKKKP"
     gene            complement(7413..7958)
                     /locus_tag="PLD53_04070"
     CDS             complement(7413..7958)
                     /locus_tag="PLD53_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRC60114.1"
                     /translation="MERPDNAREQARRLRLMAFDVDGVLTDGALYYTDEGVEIKAFNT
                     LDGHGLKMLQKFGVAVAIITGRKARCVELRMQNLGIDLLFQGVENKWETMQSLLAQLG
                     INADEAGYMGDDVVDLPVMTACGFSAAPADAHDLVKRHARMFANKAGGHGAVREVCEF
                     ILDAQGKLEDALAPYLPVSGQ"
     gene            complement(7958..8941)
                     /locus_tag="PLD53_04075"
     CDS             complement(7958..8941)
                     /locus_tag="PLD53_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRC60115.1"
                     /translation="MNQIQPSPKALDLACQVLRIEGDAVLALIGRIDDTFLEALSLIL
                     NCHGRVIVSGIGKSGHIGRKIASTLASTGTPAYFVHPAEASHGDLGMITADDVLIVLS
                     NSGESAELLNILPSVKRQGAKLIAMTGNPQSSLARDADAHLDAGVAQEACPLNLAPTA
                     STTAALALGDALAVALLDARGFGAEDFARSHPGGTLGRQLLTRIRDVMRTGEAVPTVL
                     PTTSVAEATREISRGGIGMTVVITPEREVLGIFTDGDLRRAIVNKLDFQTLTVANVMS
                     GKPRLIGPDKLAAEAVEMMEKYKINQIPVVDSNGVLIGALNMHDLFQAKAL"
     gene            9184..>11142
                     /locus_tag="PLD53_04080"
     CDS             9184..>11142
                     /locus_tag="PLD53_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HRC60116.1"
                     /translation="METLPIILMLLGAAVLSVILFRRINLPPILGYLLVGTLIGPHAF
                     NLLEGLSGAHYLAEFGVVFLMFSIGLEFSLSKLYAMKRIVFGLGCLQVTATLAVVTAV
                     LAASGLPLQVGVAIGGALAMSSTAVLTKLLSERIELDAPHGREVMGVLLFQDLAVIPL
                     LVLIPSFSQSAEQLAITMGIAMLKAIALLAPVFLLGKRLLTKWFFVVAHGKSAELFVL
                     NVLFVTLGLAWLTEAFGLSMALGAFVAGMLISETEFRHQVEADIKPFRDVLLGLFFVT
                     VGMMLDVSLVLAKWPWVLGVLMALLLVKFFLVFGLSRLFGSMSGAAMRSGLWLCAGGE
                     FGFVLLSDVSRNSVAPALIVQIVLAALVISMLMAPIIVQYSDRLVLRFAASEWLMRSL
                     QLTTIAARTLNTEKHAVICGFGRSGQYLARFLAREEVSYVALDLDPDRVRHAAAAGEN
                     VVYGDATRRETLAAAGIGRASVLIVSFIDARAAERVLRHVRELVPGLPVVVRTFDEKD
                     LDRLQRAGAAEVVPETLEASMMLASHALIHAGVPINRVLSQVQQTRAARYRTLRGFFH
                     GESDYDENVHDSDEPCLRSVSLYDGAYAIGKTLSELAVGSKGVEVMAVRRRNIRTLEP
                     SGNTRFEAGDVVVLLGTPGALVTAEGRLL"
BASE COUNT         2058 a         3124 c         3396 g         2464 t
ORIGIN      
        1 aaacgtgaat ccgactacgc ggactgcctg gccgtcgacc agcgaggttt gcgctccatg
       61 gcgcgcaaca tgcgccacgc cttcggcaag aagcgcgacg atttgcagac cgaacacgac
      121 aggcttcttg agaaatcgcg tgcggccgtc ctggtccgtc gtgtcagtct gcccaaaccc
      181 gagttcgccg acgatttgcc gatcaatgcc cgccgtggcg agattgcctc gctgatcgaa
      241 aagcatccgg tagtgatcgt ctgcggtgaa acgggttcgg gcaagacgac gcagatcccc
      301 aagatatgtc ttgaactggg ccggggaatt accggcctga tcggtcatac gcagccgcgt
      361 cgcatcgccg ctcgcgctac cgcggcgcgc attgcgcagg aactgaacag cgagctcggc
      421 gcgctggtcg gctacaagat ccgtttttcc gaccgcacct cgaaagatgc ctacgtcaag
      481 ctgatgaccg acggcatatt gttggccgaa acgcagggcg acccatggct cgggcagtat
      541 gacaccttga ttctcgacga ggcgcacgag cgcagcctga acatcgattt tctgctcggc
      601 tatatcaagc aactgctgcc ccggcgtccc gatctgaagc tgatcgtcac gtcggcaacg
      661 atcgatgccg agcgcttttc gaagcacttc aacaatgctc cggtgatcga ggtttcgggc
      721 cgcatgtatc cggtcgaaat tcgttatcgt ccggtgcaga acgaagaggc ggaaaaggaa
      781 ggcgagcgcg atctctacga cgccatcgta gatgcttgcg acgaactgag ccgaggtggt
      841 ggcattgatc acggcggcga cgtgttggtt ttcctgccgg gcgaacgcga gattcgcgag
      901 gcggccgagg cgctcagaaa acaccacccg gcgcctacgg agatcctgcc gctcttcgca
      961 cgcctgtcgg ccgaggagca gtcgcgaatc ttcaagccgc ataccgggcg ccgaattgtg
     1021 ctggcgacca acgttgccga aacctcgctg accgtgccgg gcatccgttt cgttgtcgat
     1081 acggggctgg cgcggatcaa gcgctattcc taccgcaaca aggtcgagca attgcagatc
     1141 gagaagatcg cccaggcgtc ggccaaccag cgttccggcc gatgcgggcg cgtcgccgcc
     1201 ggcgtttgca ttcgcctgta tgacgagcag gagttcggcc tgcgtccgcc gtatgccgat
     1261 ccggagattc ttcgctcctc gctggcaggc gtgatcctgc gcatgaaggc tttgcgcatc
     1321 ggcgatgtcg agagtttccc gttcatcgaa gcaccgccgc ccaaggcgat cagtgacggc
     1381 taccagctct tgctcgaact tggcgcggtg gctgacgatc gcgcgctgac accgatcggg
     1441 caggagctgg cgaagatgcc gcttgatccg cgcgtggcgc gcatgatcgt tgccgcgcga
     1501 gaagagggct gtctgcgcga gatgctggtg atcgccgccg cactgaccgt tcaggatccg
     1561 cgcgagcggc cgcaggaacg ccagggatcg gccgacgccg cacaccaaca atttgccgac
     1621 cagaaatccg aattcctttc ctggctcaag ttgtggaatt ggtacgaggg cgcggtagaa
     1681 cacaagaaat cgcagcgcaa gctggttcag gcttgccacg aaaactttct ctcggctttg
     1741 cgcatgcgcg agtggcgcga catccacaag cagttgcatg gcctcgcagc cgaacacgac
     1801 tggaaggaga atcagattcc agccacctac gaggcgatcc accgcgcact gctcgccggc
     1861 ctgctgggca atatcggctg caagtcggag gattcggcac actaccttgg cgcgcgcggg
     1921 atgaagttcc tgatccatcc cggctcggcg ctcaacaaga aagccggcaa gtggatcgcc
     1981 gctgctgaaa tcaccgaaac gaccaagctc ttcgcccgct gcgtggcgcg catcgaaccg
     2041 caatggctgg agacggtcgg tgcgcacctg atcaagcgcg gctggttcga tccgcattgg
     2101 gaaaagaagg cgatgcaggt cgtcgccttc gagcgatcga cgctctacgg cattgtcgtc
     2161 aatccgaaga agcgcgtgaa tttcggtccg ctgaatccgc aggaggcgcg cgaaatcttc
     2221 atccgccagg ggctggttgc cggggaaatt gccgaagagt atgcccggcg ctggaagttc
     2281 ttcacgcaca accaccaatt gatgctcgac attgaaacgc tcgagcacaa atcgcggcgt
     2341 ccggacgtgc tggtggacga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 acggtgccgc tggcgcagct caaccagatt ccggcagtgc agtgcgagtg gctggtgccg
     2521 ggcctgctca aggaaaaggt cattcagctg gtcaaaacgc tgccgcagaa gatacgctcc
     2581 aagctggttc ctgtgccaga attcgccgct gaattcgtgg ttgccgtgcc accttcggga
     2641 aaatcattgg tgtcgtcgtt gatccactac atcctgcaat cacgcgggct caatgcgcgc
     2701 ggatgggaga ttacgccgga tgctttccgt cccgatgcgc tgccggcgca tttcagtttc
     2761 aatttcaagc tggttgacga gcatgaccgc cagctcggca tgagccgcag cctgagcgat
     2821 ttgcggggtg agtggggcgg caaggccaaa caggagtttt ccgagctgca tgaaacaccc
     2881 tccgagtatt cggggatgac cgactggagt ttcggcgagc ttgccgaagt gatggaagtc
     2941 gaaatggctg gcggcggcag cgggcaaacg gtactcggtt atcccggtct ttcggacgac
     3001 ggcgacagcg tttcactgtg cgttttcgat tcacctgagg aagcgcgcga agcgcatgcg
     3061 actggcctcc tccggctgtt catgctgcag tttcccgacc agctcaagta cttcgagaag
     3121 aacctgccgg ggctgacgca aatggccatg gtctacatgc cgcttggttc actcgacgat
     3181 ctgcgccggc agttgatcga gctcaccttc gtgcgtgcct gcatggccga accatggccg
     3241 accgacgcgg agaatttcag gcggcgctgt gccgaagcca agacaaggct gggcctgctg
     3301 gcgcaggaaa tctgccggct ggtcgggcag atactggccg actggcaatc gttgcaaaag
     3361 agattgacgg cgtttcggag ccatgccgtc gcgttgcagg atgtcgagaa acaacttggc
     3421 cgcctgatcg gcaagcgctt cgtcgccgag acacccttcg agcgactgca gcactatcca
     3481 cgttatctca tggccatcac gcggcgactt gaaaagttga aggcgggcgg tgcacaaggt
     3541 gaactgcgcg attcgcgcct gatggctgaa tatgcgccgc tatggacgaa ttacgaacga
     3601 cgtgccatcg tactggccaa acaaggcgta agcgatccgc aagtcgagca gttccggtgg
     3661 atgcttgagg aattgcgcgt gcagctcttc gcgcaggagt tgcgcacacc ggcgcccgta
     3721 tcgagcaagc ggcttcagaa aatgtgggag ggattgtgag aatagggagg gaagcggtat
     3781 gcctgaagtg attctggcgc tacggcgaag tctggtgacg ctcggccagg gtcgcgtctg
     3841 gctctatttc ttcgggccgg cgattgttgc cttgctaatc atgatcggcc tctccgtttt
     3901 tttgctcgac tacctgatcg ccagtcttgt cgaacagcca ccgatgagct ggatcgcgtc
     3961 ctggggtgcg ttctggctgg ccaaactgtt ggctgctctc ggtggttggt tgttgattct
     4021 ctccgccagc tacttgatcg ccatgctgct gaccgccgtc gtcgtactgc cgctattgct
     4081 caatcgtctt tccacgaccg actatccaga actcgcgcgg atgggtagtg acagcgtagc
     4141 ggcggcgacc tggaacagca tttgggcggc cgtgctgttc atcatcggct ggctggtgac
     4201 cttacctctg tggctggtgc cggggcttgg gctgattctt ccgtttttct ggatggcctg
     4261 gcttaaccgt cgcacctttg cctatgattc gctggcggcc catgccacga atgacgaatg
     4321 gtgcgagttg cggcgaagac agtcgatgcc tctgctggcg ctgggtctga tcatggcgct
     4381 gctggcgcac gttcccttca tgggtttgct tgcgccgtcg ctggcggcgc tggcctatgt
     4441 tcatttctgc cttgaggcac ttcgccgcct gcgtcagggt gcggtcgtaa gcatcatcga
     4501 aaaactggag gtcagcgcat gaagttcggt gcaatcatca tcggcgacga aataatctcc
     4561 ggcaaacgta tcgaccagca cttcgccaga atcattgccc tgcttggcgc acgcggcttg
     4621 cggctatcgt gggccgaata cctgggcgat gagcgtccgc gcatcgcgga aactctgaag
     4681 cgaagctttg ccgcgggcga cgtggtattc agttttggcg gaatcggcaa cacgccggat
     4741 gatcacacgc gacaggcggc ggctgaagca ttgggtgtcg atctggtgct gcacccggat
     4801 gccgaacgcg aaatccgcgc ccgcttcggc ggtgaagtga acgcggtacg attgctgctc
     4861 ggtactttcc cgcgcggcgt cgacatcgtg cccaacccct tcaaccgcat tgccgggttc
     4921 tgcgtgcgcg atcactactt tctgcccggc ttcccgcaga tggcgcatcc aatggccgaa
     4981 tgggcgctcg ataccttcta cccgcatctg ttcaaccgtc atccgatggt tgatagggct
     5041 ttgctgctga ccgggccgaa cgcctacgag agtgcactgc tcgatctgat ggagcgcatc
     5101 gttgccgact atccggcgct tcgcctgttc agcctgccct cggtcggtga aaacggccag
     5161 cggcgtcacc ttgagctcgg tgtcgagggg gaagcgtcta tggttgatca ggccatggaa
     5221 gagattcgac aggaagttga tcgccgcaac atcgcctgga gttggagaga gtagacgctg
     5281 aggagagggg agagtaggag cggcgcgaat cgaaccgggg cgaatcgctg cgccaagaac
     5341 gacaaagccc cgctagcggg gctttgtcca tccatctgac caagaggaac gaatggccgg
     5401 atggtgtatt caggcgatta tttcttcttg gcggctgaat taactgcgtt gaccgttgca
     5461 ttgaccgtcg cattgctcgc tgtagcgatg ttggcttcag cgatctcggc agcctgcttg
     5521 acaacattct tcatgttgtc gaaagctgaa ctggcagctt cgagagccga cttgaccgca
     5581 gcgactgcaa catcagaacc tgccggggcg ttcttggcgg ctttttccat gagggtggca
     5641 acctgtttct ggaaatcgct gaactggctt tcgagttgct tggtcacttc ttctttcgat
     5701 tgtgcggtaa tttcgtagag gctgcgattg taggcaacga tctgttccac ggccggttga
     5761 atctgcgcag ccgtcatggc cagagcttca cgcggatcct tggcgcccat catggccttg
     5821 gtgttgttca ttccgtcttc catcatcgag cgggcagtat tcagattcag tgcggcgacg
     5881 cgctcggcgc tcgctagcgc agtgttggca agtgacagca gggcatcaac agaggctttg
     5941 ttggcagcaa ctaattgttc gggggtcgtg atcatggtaa atctccttag ttaaaacacg
     6001 ggtcatgagc agtgttaaac cagttattcg tcttgctgca ctgcaccatg gaccgaatta
     6061 tagcccctga atttaccttg tcaagctttt tttgatgcag tgcaacaatt tattttaagg
     6121 ctgctcaaca ctatgtttcc ttaagctgtt cgtaatcgtt cccgagcaag caaggcaatt
     6181 gctgcagtgc atttgacaag gtatgaatgg cgatcgaacc tgcgctcgcc gcttaccgag
     6241 gcgcgacagt cccggacggt ttcagcgtaa gcggctcgcc cttcggctct tcggccgcgt
     6301 tcttgccttt gggctggata atggcgcgaa cgcgagcttg tgcggtaccg gtggcgctct
     6361 tgctctcgcc gccaccgttg gttacgagat acttctcggt cagtgcgtca taggaaatgt
     6421 actggcctct gatctcatct agtccgctct tcacccaggc acgaatgaag aattcggatt
     6481 tctcgtcacg ggcatgatgc acgatgcgtt ccgcctcgcc ctcgatgtaa tcgctactgc
     6541 cttcacgttt ctgccggaag cgggccagcc cgtcggcacc ccctgtcgct acgcccttct
     6601 ggaaaccgtc agcgtcttgc gtgacaacca gtttgctggt gcgaatctga agcgttccct
     6661 gagtcaggat gacgttgccc tcaaagacct gaaccttgtt gacatcatcc atgctgatgc
     6721 gatccgcttc aagattgatc ggcttttcac gatcggcctt ttcggccagg gcgggcaacg
     6781 acagggcgag caacaggaac gtggcggcta aatagggaga atttggcttc atggcttctt
     6841 cttggcgtgt ttgctttcca gaacggcgac ggcctgggat tcgatttcgt acatctgcgt
     6901 tcgattattc acctgcatcc cgacaccttt caaccaggat tttcccctgg tgatcagcac
     6961 tgggcttttg gtgaatgctc tttcgtcatc cggaagaacg gtgagttggg gcatgtaggc
     7021 ggtcatcgct tcgtccttgg cagatgcttc gcgatgcacc cggacattgt cgtacaaatc
     7081 gacctgttcg ccatcccggc tcacgtggcc gcgttccgca ctcaagctga gcggcggctt
     7141 tttgctgtgc aggtaaacca ggtcaggttt gatcaggtcg gttgaatcat catccggata
     7201 atggcggata tctgcggcct tcagcgtgta ttgcaagcgc ccgttttgat ccagcttgtg
     7261 cagggtggag tcgctaatga tgtagtcagg gtcgtgacgg ttttttccat cgtgccgtgg
     7321 cccatccagc tcggtcgcat aacgtagcca gaaagacaga ccggtcaata ccagaagaat
     7381 gctcagcgga aagatcgcgc taccccagtt cttcattgtc cggacaccgg tagataaggg
     7441 gcaagcgcgt cttcgagttt gccctgagcg tcgaggatga actcgcagac ttcacggacc
     7501 gcaccgtgac cgccggcctt gttggcgaac atgcgcgcat ggcgtttgac cagatcatga
     7561 gcatccgccg gtgctgcgga aaaaccgcag gcggtcatca ccggcaggtc gaccacatcg
     7621 tcgcccatgt agccagcttc atcagcgttg atcccgagtt gcgccagcaa ggattgcatc
     7681 gtttcccatt tgttctcgac accctgaaat aaaaggtcga ttcccagatt ctgcatgcgc
     7741 aactccacgc aacgcgcttt ccgtccggtg atgatggcca ccgcaacgcc gaatttctgc
     7801 aacattttca ggccgtgccc gtctagcgta ttgaaggctt tgatctcgac cccttcatca
     7861 gtgtagtaaa gcgcaccatc ggtgagaacg ccgtccacat cgaaggccat cagtctcagc
     7921 cggcgcgctt gctccctggc attgtctggg cgttccatca gagggctttg gcctggaaaa
     7981 ggtcgtgcat gttgagtgcg ccgatcagca caccgttgct atccacgaca ggaatctggt
     8041 tgatcttgta cttctccatc atctcgaccg cttcggcagc gagtttgtcc gggccgatca
     8101 ggcgcggctt tccgctcatg acgttcgcca cggtgagtgt ctgaaaatca agcttgttga
     8161 ctatggcccg gcgcaaatcg ccatcggtga agatacccaa cacttcgcgt tcgggcgtga
     8221 tgacgaccgt catgccgatt ccgccgcgcg agatttccct ggtagcctcg gccacgctgg
     8281 tcgttggaag gacggtgggg acggcttcgc cggttcgcat gacatcgcgg attcgcgtga
     8341 gcaactggcg tccgagtgtg cccccggggt gtgagcgcgc aaagtcttcg gcgccaaatc
     8401 cgcgggcgtc gagcaaggcc acagccagcg cgtcacccag cgccagcgcg gcggtcgtac
     8461 ttgctgttgg cgccaggttg agcgggcagg cttcctgtgc gaccccggca tcgaggtgag
     8521 catcggcatc tctggcgagc gaggattgcg gattgccggt catggcgatc aacttggccc
     8581 cctggcgttt gacggatggc aggatattga gcaattctgc actttcgccg gagttggaga
     8641 ggacaatcaa aacgtcgtct gcggtaatca ttccgagatc gccgtgactg gcttcggccg
     8701 ggtgtacgaa gtacgccggg gtaccggtgc tggccagcgt cgatgcgatc ttgcgcccga
     8761 tgtgtccgga cttgccaatg ccgctgacga tgacgcggcc atgacagttg agaataagag
     8821 acaatgcctc caggaaggta tcgtcgatac gcccgatcaa cgcgaggacg gcgtcgccct
     8881 cgatacggag aacctggcac gctagatcga gcgccttggg agagggctga atttggttca
     8941 tggtcgagga gcggttatga ttgggtcgag tatatcagaa gccccggacg cccttgattc
     9001 cgccgcttgc cgaacctggc gacgcattcg ggaagtgtgt ggaacgagcc caggagccaa
     9061 acaggattcg gttctgccga ttggctgcct tgggatggcg tttatgcagt tctcgtgata
     9121 cgtagcgcag ggatgtcgtc ttgacggtat ccggccagca gcggttgagt ctagacggaa
     9181 acaatggaaa cactaccgat cattttgatg ttgctgggcg ctgcggtgct gtcggtcatt
     9241 ctgtttcggc gcatcaactt gccgcctatc ctcggttatt tgctggttgg caccttgatc
     9301 ggcccgcatg cgttcaacct cctcgaaggg ctttcgggag cgcattatct ggcagaattc
     9361 ggcgtcgttt ttctgatgtt ctcaatcggc ctggaatttt ccctatccaa gctctacgcg
     9421 atgaagcgca ttgtttttgg cctgggttgc ctgcaggtga cggcgactct cgccgtcgtg
     9481 accgcagtgc tggcagcatc gggattgcca ttgcaggtcg gtgtggccat cggcggagcg
     9541 ctggcgatgt cctcaaccgc agtgctgacc aaactgctga gcgagcggat cgagctggat
     9601 gcgccacacg ggcgtgaagt catgggcgtg ctgctttttc aggatctcgc ggtcatccca
     9661 ctgctcgtcc tgatcccatc gttttcacag tcagccgagc aattggcaat caccatgggc
     9721 atcgccatgc tcaaggcgat tgcgcttctc gccccggtgt ttcttctcgg caaacgtttg
     9781 ttgaccaagt ggttcttcgt cgtcgcacat gggaagtcgg ctgaactctt tgtgctcaac
     9841 gtgctgttcg tcacgctcgg tctggcctgg ctgaccgaag cgtttggctt gtcgatggcg
     9901 ctcggtgcct tcgtcgccgg gatgctgatt tcggaaaccg aattcagaca tcaggttgaa
     9961 gcggacatca agccttttcg tgacgtgcta ctgggcctgt tcttcgtaac cgtgggcatg
    10021 atgctcgatg tctcgctggt tcttgcaaag tggccgtggg tgctcggcgt gctcatggcg
    10081 ctattgctgg tcaagttctt tctggttttc ggtttgtcgc ggctcttcgg atccatgagt
    10141 ggtgccgcga tgcgttccgg cctgtggttg tgtgcgggtg gagaattcgg ctttgtcctg
    10201 ctctcggatg tcagccggaa ttccgttgca cctgcgctga tcgtccagat cgtactggcg
    10261 gcgctggtta tctccatgct catggctccg atcattgtgc agtacagcga tcggctggta
    10321 ttgcgctttg ccgccagcga gtggctgatg cgctcgctgc agctgaccac gattgccgcg
    10381 cgtaccctga atactgaaaa gcatgcggtg atatgcggtt tcgggcgtag cggccagtat
    10441 ctggcccgct tcctggcgcg cgaagaggtt tcctatgtcg cgctcgacct cgacccggat
    10501 cgcgtgcggc atgcggcggc agcgggtgaa aacgttgtct atggcgacgc cacccggcgc
    10561 gaaaccttgg cggcggcggg gatcggtcgc gccagcgtgc tgatcgtttc gttcatcgat
    10621 gcacgcgccg cagagcgtgt gctgcgtcat gttcgagagc tggttcccgg attgcccgtg
    10681 gttgtgcgta ccttcgacga aaaggatctc gatcgcctgc agcgagccgg agcggcagaa
    10741 gtcgttccgg aaacgcttga agcatcaatg atgttggcat cgcatgcgct gattcacgcc
    10801 ggggtgccga tcaaccgtgt gctcagtcaa gtgcaacaga cgcgggccgc gcgctatcgc
    10861 acgttgcgcg gcttcttcca cggcgagagc gactatgacg agaatgtgca cgatagcgac
    10921 gaaccgtgtt tgcgctcggt atcactgtac gacggtgcct acgccattgg taagacattg
    10981 agcgagcttg cggtgggcag caagggcgtc gaggtgatgg ccgttcggcg gcgcaacatc
    11041 cggacgctgg aaccttccgg gaacactcgc ttcgaggccg gtgatgtggt tgtgctgctg
    11101 ggtacgccag gggcgctggt cactgcggag gggaggttgc tc
//
LOCUS       DAORPM010000053        11100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1316_length_11100_cov_10.801811, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000053 DAORPM010000000
VERSION     DAORPM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1316_length_11100_cov_10.801811"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLD53_04085"
     CDS             <1..184
                     /locus_tag="PLD53_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60117.1"
                     /translation="ITQLAWGLLPILALVIVDLQVHRVDWLACLLWIWIGMFVMTAAM
                     GVWMLRMAASESVSSR"
     gene            complement(451..1770)
                     /locus_tag="PLD53_04090"
     CDS             complement(451..1770)
                     /locus_tag="PLD53_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRC60118.1"
                     /translation="MKTILISLLAVVAAAWGLALTVTNFPEHSALPWILRQQGLYLTG
                     LFSIFLMSLSMLLATRPAWLEKPLGGLDRIYRLHKWSGILAIGFGASHWLIKQSGGLI
                     KTLIGAQGRLPKIRYTGLLEVLREFAADLGEPALYIVLAMLVITLWRRFPYHIWRHLH
                     RIMPVLYLMLAFHAMMLAPPAYWSQPVGALLAVVLAVGVVASVIVLAGLIGRRRQVNG
                     SVVSVKNEGDVTELTCRLDEQWKSHRAGQFAFVTFDWIEGAHPFTIASADRGDRVITF
                     YIKALGNYTQDLAQRVRAGQKVKIEGPYGCFQLDRNNPGSRQIWVAGGVGVTPFLSWL
                     ESLQTHPAEARDADLHYCTRNREGDPFVARLQALCAALPGIRLYVHSDRHGDVLTADS
                     LKLAEGQHKKAEVWFCGPNGLANALKKGLPDSWNGRLRFHQEAFELR"
     gene            complement(1793..2284)
                     /locus_tag="PLD53_04095"
     CDS             complement(1793..2284)
                     /locus_tag="PLD53_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HRC60119.1"
                     /translation="MNIPYRPIVLGLLGMVCSGIFLQRALAGGEHYFPPVTDPVVREE
                     CGSCHLAYAPSMLPASSWKRMMGELDKHFGTDASVAPATAERITGYLVENAADTGGQR
                     YGGKLMRGVSLENPPLRITELPKWVREHNGISTRTWKRKDVRTKSNCLACHVDAERGY
                     YDE"
     gene            complement(2301..2714)
                     /locus_tag="PLD53_04100"
     CDS             complement(2301..2714)
                     /locus_tag="PLD53_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HRC60120.1"
                     /translation="MNPAFVTVALIALMLPVAGLASPREELLSQYAAAAKAASPGFSG
                     FSASRGEKLHFATFHSGKPDTPSCTSCHGNDPRRAGRTTTGKPIDAVAISVTPTRYSD
                     PAKVEKWFKRNCNDVLGRECTALEKGDWLSYMISQ"
     gene            2847..3200
                     /locus_tag="PLD53_04105"
     CDS             2847..3200
                     /locus_tag="PLD53_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRC60121.1"
                     /translation="MGATGKCRKVLAALAAGLLLAPILPGHADDWRDGRRDHDRARRA
                     LEAGEILPLDTVLERVARDTPGRVMEVELEREHGRWVYEIRLLRSGGVLIKLLVDARD
                     GTVIKQRDYGPRANH"
     gene            3218..3883
                     /locus_tag="PLD53_04110"
     CDS             3218..3883
                     /locus_tag="PLD53_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC60122.1"
                     /translation="MRILVVEDEPSIAAQLSEALTAAGYAVDVADNGVDAHWQAVDEG
                     QPFDAVVLDLGLPQMDGLTVLKKWRAAGRSMPVLILTARDNWHEKVAGIDAGADDYLT
                     KPFHMEELLARLRALIRRAGGHASAELVCGDIVLDTRHSRVTVGGLALTLTSHEYRVL
                     DYLMHHPGQLVSRSDLIEHIYAQDFDRDSNTVEVFIARLRKKLPADAIETVRGLGYRL
                     VVP"
     gene            3907..5298
                     /locus_tag="PLD53_04115"
     CDS             3907..5298
                     /locus_tag="PLD53_04115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRC60123.1"
                     /translation="MKYNTMALRGSLRLRLLLGTLVWIAVSIIVAGWMLGGLFRQHVA
                     RQFHTELNTHLDQLAAHLAVDQAGQLSLSATPSDPRFSKPHSGMYWQVDRIGVMPGDA
                     AGILRSRSLWDEVLKVPSDTLADGEIHQHRVPGPGKKMLGMVERVITLADAPDQSAKT
                     FRLIVAADEHQMAEPIEHFDQMLWLALGLLGGGLALSAVIQVLIALLPLRRMQGALAA
                     VRSGQTNRLEGQFPQEVRPLVEDFNSVLTQNAEVVERARTQAGNLAHALKTPLSVIAN
                     ATSSRADDTAQLVSEQVAVARKQIDYHLSRSRLAASVRVPGARTSVVPVLDGLVRVMR
                     RIHAERNLELIVRPHNELPDFRGEEQDLQEMLGNLLDNACKWAAKRVDVSLAVAADEL
                     LIMIDDDGRGIAEAEQQRVLNRGERADERVPGSGLGLAISVDLAHLYGGRVALAVSPL
                     GGLRVVLALPKAA"
     gene            5596..6567
                     /locus_tag="PLD53_04120"
     CDS             5596..6567
                     /locus_tag="PLD53_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60124.1"
                     /translation="MKIEQSAVSMNADHRLTSEYEARLESRISFRTLFDGVAQTGESP
                     ASGETDRQTRLLMMIEALLARLLALISGKHNSLMSDVCALLGTEIPKGGAGRPLRALE
                     MEWNNEFTQIFREHERSDFSSTGKIRTADGRSLDFKLELAMCRNFECERKAVDSGKAV
                     VLRDPLVINFDGKATELSGKRFAFDLDADGKSESIDTLGSCSGYLAIDRNGDGRINDG
                     SELFGTRSGNGFADLAAFDGDGNHWLDEADAAFATLRIWQPDGVGQDMLSTLRDKGVG
                     ALYLGSAETPFSLTDSDNRPLAQIRASGIYLCEDGSVGTLQQVDLAV"
     gene            6807..8171
                     /locus_tag="PLD53_04125"
     CDS             6807..8171
                     /locus_tag="PLD53_04125"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRC60125.1"
                     /translation="MKPLVPLFLVLLLGLVTPGQARDAGPNPWEVLGDVLRKGKPAPV
                     DAARPAAPVSKVEASELWDAFLTNVVKRSAGESGDASRRQEFLFVLLSGRYDGLSMLA
                     SEAPVPDPLRELFVLSWDRLAPALRNLAGDLDAKAANPFRALAEAGDALKKAQGFGDS
                     LGIHITPETLRQLARLVLPAKAGDPLAYDLALDPELRRVFGFGAPLPPAEPSLLLSVS
                     LPSLPSNPLSWLMPTAFAAPLAAPLPFDPETAALAKRLNNWLPTPSELPDYLLEMRTL
                     LQRTADTTLKQRESAGRALAEPFHALYRHLVLATAWQESCWRQFVRRKGKVQPIQSGV
                     GAVGLMQVYPVVWRGFYDVTGLQTDVGYNGRAGAEILHHYLRDYAIGKREHTAVGDAD
                     NLARGTYAVYNGGPGHLTRISKPKLRADLREIDNSFFEKYQAVKAGNELDVGKCFTGL
                     DLPR"
     gene            complement(8297..8701)
                     /locus_tag="PLD53_04130"
     CDS             complement(8297..8701)
                     /locus_tag="PLD53_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HRC60126.1"
                     /translation="MPNQVQWNPRYSVGNEILDDQHQAILAQCNALADHVVGTDPESD
                     LKFHTLFNELTARAREHFSSEEDLLVDCACPTLEEHRNECDEFEYLVAEIVTTDNFDK
                     LELQKFLIFWWVGHILDSAERYRACAASSPTD"
     gene            complement(8719..9141)
                     /locus_tag="PLD53_04135"
     CDS             complement(8719..9141)
                     /locus_tag="PLD53_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60127.1"
                     /translation="MSDSSEKPVRGDPTWVAPALTKSAKTRVVGKTNVFFGPPLAILA
                     EGQKNTMEISGKLNRTAERYMEILKHHGLELTEPERLCLAHICQIGFMSPLEISELPF
                     EVGLTRFECDGLDKVALATKLEAVSFADLVAVVESLGF"
     gene            complement(9152..9994)
                     /locus_tag="PLD53_04140"
     CDS             complement(9152..9994)
                     /locus_tag="PLD53_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60128.1"
                     /translation="MKKLAIYSVVVALCGSTLYAVFAAEKPAAAKEAAAQAEPAKEEA
                     AKEPLVCFESRKGASEITQKDVKPGWPNVKCSPTTGAALWYGDPFDSTVPMGNIPQMD
                     IPEGKAVVKPRSESLALMPMCGTACHNGVGPQFTPPKLPKDKRPAPIPTMAAMVPDLA
                     NFEHGRGRIWCMDCHHTTQRNKLVDHFGDPISFDQPQLLCGKCHGDKLRDWRDGIHGK
                     RIGEFVSTGKKRWFTCTECHNPHNVQDGARNRGFVKLQPEPPPQLSRGMKDAGHEKLH
                     PIPH"
     gene            complement(10005..>11100)
                     /gene="nrfD"
                     /locus_tag="PLD53_04145"
     CDS             complement(10005..>11100)
                     /gene="nrfD"
                     /locus_tag="PLD53_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRC60129.1"
                     /translation="TDGLIVTGMTSQISDGLYLANLVFLVGVAAGAVTIVFPAYVYHH
                     EGMHKVAVLGEMLAISAVIMVMVFVFAHMGRPDRLWHMIPPLGIYSFSSMLGWDVLVL
                     NGYLVLNAICGFYYLWCKYTGKPVNSKWFIPLVYVAIAWALSIHTVTAFLMANNPARP
                     MWFHSMMPIRFIATAFAAGPCLIILIFLIIRNNTRFWVDDSAIKLLTTIVQFCLGIAI
                     FLTMSEVVVELYARTEHANGLYYLMFGLHGLTGLVPWFWSSVVLMIVAFVMLFMPSFR
                     NDFNKLQIPCTMAFAGIWIEKGMGLIVPGYIPSPIGEVTEYHPSFVEWLLTLGIWAFG
                     FFILTILLKGAIGILLGEIKYGGTQAVAAK"
BASE COUNT         2120 a         3214 c         3555 g         2211 t
ORIGIN      
        1 catcacgcaa ctggcctggg ggctgctgcc gattcttgcg ctggtgatcg tcgatctcca
       61 ggtgcatcgc gtggattggc tcgcgtgtct gctgtggatc tggatcggga tgttcgtgat
      121 gacggcagcg atgggcgtct ggatgctgcg gatggcagca agcgagtctg tgtcttccag
      181 gtgaaggccg ccggattagg gtggcttttg aattccgttg atgtccgatg acctggcaag
      241 gggcaatcgc ccagcctgcc tgcatcgctg cattgcagta gagtcgcgcc tttaccgagc
      301 cgcagatgat tccaattcca tttcatcagt gatattgcgt tggctttcga attcgctcct
      361 agccgtgcca aatgcactgt ctttgtcgct catttgtctc cgccttgtct cactttcgaa
      421 ctgggattgg aatggcatcc agctgaccga ctagcgcagc tcgaaagctt cctgatggaa
      481 gcgcaggcga ccgttccagg aatccggcaa gcccttcttc aaggcgttgg cgagcccgtt
      541 gggcccgcag aaccagactt cggctttctt gtgttggcct tcggcaagtt tgagcgagtc
      601 agccgtcaag acatcgccgt gcctgtcgct atgaacgtac agccgaatgc cgggcagcgc
      661 ggcgcatagc gcttgcaggc gggcaacgaa cggatcgcct tcgcgattgc gcgtgcagta
      721 gtggaggtcg gcatcgcgtg cttcggcagg atgcgtttgc agcgattcca gccaggacag
      781 gaagggcgtt acgccaactc cgccggccac ccaaatttga cggctgccgg ggttgttgcg
      841 gtcgagctgg aagcagccgt aaggcccttc gatcttgacc ttctggccgg cgcgaactcg
      901 ttgcgcaaga tcttgcgtgt agtttcccag cgccttgatg tagaaggtga taacccggtc
      961 gccccggtcg gcgctggcga tggtgaaggg gtgcgcgcct tcgatccagt cgaaggtgac
     1021 gaaggcgaac tgcccggcac gatggctctt ccattgttca tcgaggcggc aggtgagttc
     1081 ggtcacatcg ccttcgttct tgaccgagac tacggagcca ttgacctggc ggcgacgccc
     1141 gatcaatccg gccagcacga taacgctggc aaccacgccg accgcaagca caacggccaa
     1201 cagcgcgcca accggttgcg accagtaggc gggcggtgcc agcatcatcg cgtgaaaggc
     1261 cagcatcagg tacagcaccg gcatgatccg gtgcagatga cgccagatgt ggtaagggaa
     1321 acggcgccac aaggtgatga ccagcatcgc caggacgatg tacagcgccg gttcgccgag
     1381 gtcggcggca aactcgcgca acacttcgag caatccggtg tatctgattt tcggcaagcg
     1441 gccttgcgcg ccgataagcg ttttgatcaa tccgcccgac tgcttgatca gccagtggct
     1501 ggcgccaaaa ccgatggcca ggatgcccga ccacttgtgc agtcgataaa tgcggtccag
     1561 tccgccgagc ggcttttcca gccaggccgg gcgggtggcc agaagcatgg aaagcgacat
     1621 caggaaaatc gagaacagcc cggtgagata gagtccttgc tgacgaagga tccatggcag
     1681 agcgctgtgc tccggaaaat tcgttactgt cagtgccagt ccccaggcgg cagcaacgac
     1741 cgccagcagg gaaataagga tggtcttcat tgtgtgttct tattcaggtt tgttattcgt
     1801 catagtaacc gcgctcggcg tcgacatgac aggccaggca attggacttg gtgcgcacgt
     1861 ccttgcgctt ccatgtccgg gttgaaatcc cgttgtgctc acgcacccac ttcggcagct
     1921 cggtaatgcg caatggagga ttctccaagg acacgccacg catcagtttt ccgccatagc
     1981 gctggccgcc ggtatccgcg gcattttcga cgaggtagcc cgtgatccgt tcggcggtgg
     2041 ctggagcaac actggcatcg gtgccgaagt gcttgtcgag ttcgcccatc atgcgcttcc
     2101 aggaactggc cgggagcatc gacggcgcat aagcgaggtg gcaactgcca cattcctcgc
     2161 gcacgaccgg gtcggtcacc ggcgggaagt aatgctcgcc tccagccagc gcccgttgca
     2221 ggaaaatccc cgagcagacc atgccaagaa gaccaagaac gatggggcga tagggaatat
     2281 tcatggtggt ttcctttcgt ctattgactg atcatgtagc ttagccagtc gcccttttcg
     2341 agcgctgtgc attcgcggcc gagaacgtca ttgcagttgc gcttgaacca tttttcaacc
     2401 ttcgccgggt cggagtaacg ggtgggcgtg accgagatgg cgactgcatc gattggcttg
     2461 ccagttgtcg ttcgcccggc acggcgcggg tcgttgccgt ggcaggacgt acagcttggc
     2521 gtatccggct tgccgctatg aaacgtcgcg aaatgaagct tctcgccacg tgacgcagaa
     2581 aagcccgaaa atcccggcga tgccgctttg gctgcggcgg catactggct caacagttct
     2641 tcgcgtggcg aggcaagacc ggcgacaggc agcatcaagg caatcagtgc gacggtgaca
     2701 aatgctgggt tcaagaggta ctcctttcat ggtgttgtac gtactttgcc gtgccgtgcc
     2761 tgaacacagg ctgaattgtc tgttcatcag cagttcagct tcgccgggtt acaagggggc
     2821 agttgatcga caattcagga ggcgcgatgg gagcgacagg caaatgcaga aaggtactgg
     2881 cggcgctggc ggccggactg ctccttgccc cgattctgcc cggccacgcc gacgattggc
     2941 gcgacggtcg gcgcgaccat gatcgcgcac gccgggcgct tgaagccggc gagattctgc
     3001 cgttggatac ggtgcttgaa agggttgcca gggatacacc gggccgcgtc atggaggtcg
     3061 agctggaacg cgagcacggg cgctgggtgt atgaaatccg gttgttgcgt tcgggtggcg
     3121 ttttgatcaa gctgttggtt gatgcccgtg atggaacggt catcaagcag cgggattatg
     3181 gtccgcgtgc aaatcactga tcgggagata cggacgaatg cgcattctgg ttgttgagga
     3241 tgagcccagc attgcggcac agctcagcga ggcactgact gctgccgggt atgcggtcga
     3301 tgtggcggac aatggagtcg atgcgcattg gcaggcggtc gacgagggcc agccttttga
     3361 tgcagttgtt ctcgatctgg gcctgccgca gatggatggg ctcaccgttt tgaagaaatg
     3421 gcgggccgcc gggcgaagca tgccggtact gattctcacc gcgcgcgaca actggcacga
     3481 gaaagtggcc gggattgacg ccggcgccga tgattacctg accaaaccat ttcatatgga
     3541 agaactgctg gctcgactgc gtgcgctgat tcgtcgggca ggcggccacg ccagcgccga
     3601 actggtgtgc ggcgatatcg tgctcgacac ccggcacagt cgcgtcaccg tgggtgggct
     3661 agcgctgacg ctgaccagcc acgaataccg cgttctggac tatctgatgc atcacccggg
     3721 acagctggtt tcgcgcagcg atctgatcga acacatttat gcccaggact ttgatcgcga
     3781 ttccaatacg gtcgaagtct tcatcgctcg attgcgcaag aaactgcccg cagacgcgat
     3841 cgaaacggtg cgtggtctcg gctatcgtct cgtcgtgcca tgaagggtgc gagtacgggc
     3901 gaaggtgtga aatacaacac aatggcgttg cgtggctcgc tgcgcctgcg cctgttgctc
     3961 ggtacactgg tctggatcgc cgtgtcgatc atcgtcgccg gctggatgct gggtggcctg
     4021 ttcaggcaac acgtggctcg gcagtttcac accgaactca atacgcatct cgatcagctc
     4081 gcggcacatc ttgcggttga tcaggcaggg cagttgtccc tgtctgccac gccaagcgat
     4141 ccgcgcttca gcaaaccgca ttcgggcatg tactggcagg tcgatcgaat cggtgtcatg
     4201 ccgggcgatg ccgcgggcat tctgcgttca cggtcgctct gggacgaggt gctcaaggtg
     4261 ccgtccgaca ctctggccga tggcgagatt catcagcatc gcgttcccgg ccccggaaag
     4321 aaaatgctgg gcatggtcga gcgcgtgatt acccttgccg acgcgccgga tcaatccgcc
     4381 aaaacgttcc gcttgatcgt tgccgccgat gaacaccaga tggccgagcc gatagagcac
     4441 ttcgaccaaa tgctctggct ggcgctgggt ctgctgggcg gcggactggc gctctccgca
     4501 gtgatccagg tgttgatcgc gctgttgccg ctcaggcgaa tgcagggcgc cctggcggcg
     4561 gtgcgcagtg ggcaaacaaa ccggttggaa ggccaattcc cgcaggaggt ccggcccctg
     4621 gtcgaagact tcaacagcgt actgacgcag aacgccgaag tggttgagcg ggcgcgaacg
     4681 caggctggaa atctggcgca tgctctgaag acgccgttga gcgttatcgc caatgcaacc
     4741 agttcccgtg ccgacgacac ggcgcaactg gtttcggagc aggtcgcagt ggcgagaaaa
     4801 cagattgact atcatctgag ccggtcaaga ctggccgctt cggtacgcgt tcccggcgca
     4861 cggacgtcgg ttgtgcccgt gctcgacggt ttggtgcggg tgatgcggcg aatccatgcg
     4921 gagcgcaacc tcgaactgat cgtacgccca cacaacgagc tcccggactt ccgcggagaa
     4981 gaacaggatc tacaggagat gctgggcaac ctgctcgata acgcctgcaa gtgggcggcg
     5041 aagcgcgttg acgtgtcgct ggcagtcgcg gctgacgaac tcctgatcat gatcgacgac
     5101 gacggtcgcg gcatcgcgga agcggaacag cagcgagtcc tgaaccgtgg cgagcgggcg
     5161 gatgaacgcg tgcccggatc cggtctcggt ctggcgatca gcgtcgatct ggcacacctt
     5221 tacggcgggc gggtcgcgct ggcggtttca ccgctgggcg gattgcgagt ggtgctggcg
     5281 ctgccgaagg ctgcctagca tcacgaagac gtagacgacg aaaaccctgc tcgtaaagac
     5341 ggtcaccagc cgctcagggg caaacgacct caatccggcg attcagtgaa cgaccactcc
     5401 tcttgccggt ccgatcgcga ccggcacgtt gcagacggtg tatttcgcca ccgatcccgc
     5461 cgccgttgcc gcccgaccgg cgaagtttgc cgcacgttat cgcctgacct gcgcggggcc
     5521 atgatttcct gatggctcaa gcgatggcat gcaccttgca gcagtctgtt tcccctgact
     5581 agcaaggagt gagaaatgaa gatcgaacaa tccgccgtgt cgatgaatgc cgaccatcgg
     5641 ctcaccagcg aatacgaggc caggctcgaa tccagaatca gcttccgcac tcttttcgat
     5701 ggcgtggcgc agaccggaga atcgccggcg agtggcgaaa cggatcgcca gactcgtctc
     5761 ctaatgatga tcgaggccct tcttgcccga ctactggcat tgatttccgg caagcataat
     5821 tcattgatga gcgacgtgtg cgcgctgctc ggaacggaga taccgaaagg cggcgccgga
     5881 cgtccgctac gggcgctcga gatggaatgg aataacgaat tcacccaaat ctttcgcgaa
     5941 cacgagcgtt ccgatttttc atcgacaggc aaaatccgta cggccgatgg ccgttcgctc
     6001 gacttcaagc tcgaactggc catgtgtcgc aacttcgagt gcgagcgaaa ggctgtcgat
     6061 agcggcaagg cggtagtact gcgcgacccc ctggtgatca acttcgacgg caaggccacc
     6121 gagttatcgg ggaagcgctt cgcgttcgac ctcgacgcgg atggcaagag cgaatcgatt
     6181 gacactctgg gcagttgcag cggctacctg gccatcgatc gcaacggcga tggccgcatc
     6241 aatgacggca gtgaactgtt cggcacccgc tccggaaacg gctttgccga ccttgcggcc
     6301 ttcgacggcg acggcaacca ctggctcgac gaggccgacg ccgcgttcgc cactctgcgc
     6361 atctggcagc ctgatggcgt cgggcaggat atgctgagta ccttgcgtga caagggtgtc
     6421 ggcgcgctct atctgggttc ggccgagacg cccttctcgc tcaccgacag cgacaaccgg
     6481 ccgcttgcgc agatccgcgc gagcggcatc tacctgtgcg aagacggcag cgtcggtacg
     6541 ctgcaacagg ttgatctggc tgtttagcgg cacttggctc cggaagaggg gtgggtgatc
     6601 agcgattacc ataaagtggg ttgtgattca gacgccatgg gggattttga gttgaaacga
     6661 gacattgacc ggagcacggc ggtcgatgat actcaattcg aacagaaatt gaccggggtc
     6721 cggtgtttga gaatactcag ggccgggccc tgaaattacc actatggtga cgcggccgtg
     6781 agattactga ccctgggaat atcgccatga agcctctcgt tccgctgttc ctcgttctgc
     6841 tcttgggtct ggtgacgcct ggtcaggcgc gcgacgccgg gccaaatccc tgggaggtgc
     6901 ttggcgacgt gctgcgcaag ggcaagccgg caccggtgga cgctgcgcgc ccggccgcgc
     6961 cggtctcgaa ggtcgaggcg tccgagttgt gggatgcctt tctgacgaat gtggtcaagc
     7021 gctctgcggg cgagtcgggt gatgcgagcc ggcgccagga gttcctgttc gtcttgctca
     7081 gcggccgata cgacggcttg tcgatgctgg ccagcgaggc ccccgttccc gacccgctgc
     7141 gggagctttt tgtgctgagc tgggatcgac tcgcaccggc ccttcggaat ctagccggcg
     7201 accttgacgc gaaagcggcg aatccctttc gggcactcgc ggaagccggt gatgccttga
     7261 agaaggcgca aggtttcggc gattctctgg gcatccacat tacaccggaa actttgcgcc
     7321 aactggcccg cttggtcctg cccgcgaagg ccggcgaccc gctcgcctac gatctcgctc
     7381 tcgaccccga gctgcgccgc gtcttcggct tcggcgcccc gctaccgcct gccgagccaa
     7441 gcctgctgct cagcgtcagt ctgccctcgc taccgagcaa ccccttgagc tggctgatgc
     7501 cgacggcctt cgcggcgcca ctcgcagctc cgctgccgtt cgaccctgag acggctgctc
     7561 tggccaagcg tctgaacaac tggctgccga cgccaagcga gttgcctgac tatttgctcg
     7621 agatgcgcac actgctacag cgcacggccg atactacgct caagcagcgc gagagtgcgg
     7681 ggcgcgcgct tgccgagccg ttccacgcgc tctatcgcca tctggtgctc gccacggcct
     7741 ggcaggagag ttgctggcgt cagttcgtgc gccggaaggg aaaggtgcag ccgatccagt
     7801 cgggagtggg agcggtcggg ctgatgcagg tatacccggt cgtctggcgc ggcttttacg
     7861 atgtcaccgg gctgcagacc gacgtcggct ataacggtcg cgcaggcgcc gaaatcctgc
     7921 atcactactt gcgcgactac gcgatcggaa agcgcgagca caccgctgtc ggcgatgccg
     7981 ataatctcgc ccgtggtacc tacgcggtct ataacggtgg gccaggtcat ctgacgcgca
     8041 tcagcaagcc gaagctgcga gccgacctgc gcgagatcga caactcgttc ttcgagaagt
     8101 atcaggcggt gaaggccggc aacgagttgg atgtcgggaa atgcttcact ggcttggact
     8161 tgccgcgatg aaacggcgta ggtgcgctac ggtagaccac gaggatagca gttgtaccga
     8221 gcccagaacg ccgtgggcaa ttgcggcgta gatcatctcg attgtttcgg gcgcggcttt
     8281 ttcagcgccg cgggctctag tcggttggcg aactggcggc gcaggcgcga taccgctcgg
     8341 cggagtccag gatatgtccg acccaccaga aaatcagaaa tttctggagt tcgagtttgt
     8401 cgaagttatc cgtcgtgacg atctcggcca ccagatattc gaattcgtcg cactcgttcc
     8461 tgtgctcttc gagcgtgggg caggcgcaat cgacgagcaa gtcttcctcg ctcgagaagt
     8521 gctcgcgcgc tcgagccgta agctcgttga agagcgtgtg gaatttcagg tcgctctccg
     8581 ggtcggtgcc aacgacgtgg tcggcaaggg cattgcattg cgccagaatc gcttggtgct
     8641 gatcgtcgag aatttcattc cccacgctgt agcgtggatt ccattgcact tgattcggca
     8701 cggccaatct cccgttcgtc aaaatccaag cgactcgacc acggcgacga ggtcggcgaa
     8761 gctgacggct tcgagctttg ttgccagagc caccttgtcc agcccatcgc attcgaatct
     8821 ggtcagaccg acctcgaatg gtagctcgct gatctccagc ggcgacatga aaccaatttg
     8881 gcagatatgg gcaaggcaca gacgttcggg ttcggtgagt tccaatccgt gatgcttaag
     8941 aatttccatg tatcgttcgg ctgtgcggtt gagcttgccg ctgatttcca tggtgttctt
     9001 ttgcccctcc gcgagaatcg cgagcggagg cccgaagaag acgttggtct ttccgacaac
     9061 tctggttttt gccgacttcg taagggcagg ggcaacccag gtggggtcgc ccctgaccgg
     9121 tttttcggat gagtccgaca tcagcgctac atcagtgcgg aatcggatgc agcttctcgt
     9181 gcccggcgtc cttcatgccc ctcgacagct ggggcggcgg ctccggctgc agcttgacga
     9241 agccgcgatt gcgcgcgcca tcctgcacgt tgtgcgggtt gtggcactcg gtgcaggtga
     9301 accagcgctt cttgccggtg ctgacgaatt cgccgatgcg cttgccgtgg atgccgtcgc
     9361 gccagtcgcg cagcttgtcg ccgtggcact tgccacacaa gagctgcggc tggtcgaagc
     9421 tgatcggatc gccgaagtgg tcgaccagct tgttgcgctg cgtagtgtgg tggcagtcca
     9481 tgcaccagat gcggccgcgc ccgtgctcga agttcgcgag gtcgggcacc attgcggcca
     9541 tggtcggaat cggcgccggc ctcttgtcct tcggcagctt gggcggcgtg aactggggac
     9601 cgacgccgtt gtggcaggcg gtgccgcaca tcggcatcag cgccagactt tccgagcgcg
     9661 gcttgaccac cgccttgccc tccggaatgt ccatctgcgg tatgttgccc atcggcacgg
     9721 tgctatcaaa ggggtcgccg taccagagcg cggcgccggt ggtcggcgag cacttgacgt
     9781 tgggccagcc gggcttgacg tccttctggg tgatctcgga ggcgcccttg cggctttcga
     9841 agcagaccag cggctctttc gcagcttcct ccttggcggg ttccgcctgc gccgcggcct
     9901 cttttgcagc tgccggtttt tccgcagcga agacggcgta gagggtgctg ccgcacagcg
     9961 cgacaaccac agaataaata gcaagttttt tcatgatcgt tgcctcactt cgctgcaacg
    10021 gcctgggtgc cgccgtattt tatttcgccc agaaggatgc cgatggcgcc cttcaacagg
    10081 atggtcagga tgaagaaacc gaaggcccag atgccgaggg tcagcagcca ctcgacgaag
    10141 cttgggtggt actcggtgac ttcgccgatc ggcgagggga tgtagccggg gacgatcaag
    10201 cccatgccct tctcgatcca gatgccggca aaggccatcg tgcagggaat ctgcagcttg
    10261 ttgaagtcat tgcggaacga cggcatgaag agcatcacga acgcgacgat catcaggacc
    10321 accgaactcc agaaccatgg caccagcccg gtcaggccgt gcaggccgaa catcaggtag
    10381 taaaggccgt tggcgtgctc ggtgcgggca tagagctcga cgacgacttc ggacatggtc
    10441 aggaagatgg cgatacccag gcagaactgg acgatcgtgg tgagcagctt gatcgcgctg
    10501 tcgtcgaccc agaatctggt gttgttgcgg atgatcagga agatcaggat gatcagacag
    10561 gggccggcgg cgaaagcggt ggcgatgaag cggatcggca tcatgctgtg gaaccacatc
    10621 gggcgcgccg ggttgttggc catcaggaag gccgtcacgg tgtgaatcga cagagcccag
    10681 gcgatggcga catagaccag ggggatgaac catttgctgt tgaccggctt gccggtgtac
    10741 ttgcaccaca ggtagtaaaa gccgcagatg gcgttgagaa cgagatagcc gttgagtacc
    10801 agcacgtccc agcccagcat cgacgaaaag ctgtagatgc ccagtggtgg aatcatgtgc
    10861 cagaggcggt cggggcggcc catgtgggcg aagacgaaca ccatcaccat gatcaccgcc
    10921 gagatagcca gcatctcgcc cagaacggcc accttgtgca tgccttcgtg atgataaacg
    10981 taggccggga agacgatggt caccgccccg gcggccacac ccaccaggaa caccaggttg
    11041 gccaagtaca ggccgtcgct gatctggctg gtcatgccgg tgacgatcag gccgtcggtg
//
LOCUS       DAORPM010000054        11064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1325_length_11064_cov_12.903715, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000054 DAORPM010000000
VERSION     DAORPM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1325_length_11064_cov_12.903715"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLD53_04150"
     CDS             complement(<1..245)
                     /locus_tag="PLD53_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HRC60130.1"
                     /translation="MKASKLLFGLVAVFLSAVPPLASAQEPIVIKFSHVVANDTPKGK
                     AADFFAKKAGELTKGKVKVEVYANSTLYKDKEEMEAL"
     gene            734..2173
                     /locus_tag="PLD53_04155"
     CDS             734..2173
                     /locus_tag="PLD53_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRC60131.1"
                     /translation="MSKDFFNEMYDAAGALRPHYRAYADWLAATPPERIARKRAEADI
                     AFHRVGITFAVYGEESSQDQGKERLIPFDIIPRIIPAAEWSALQAGLRQRVKTLNAFL
                     HDIYHKQEILQAGLIPREQVLNNAQFRPEMMGVDVPAGIYAHIAGVDLVRAGEGEYYV
                     LEDNLRVPSGVSYMIEDRKMMMRLFPELFSRHKIAPVQHYPDMLLENLRTVAPAGVLN
                     PTVVLLTPGAYNSAYFEHTFLAQQMGIELVEGRDLFVRDETVFMRTTQGPQRVDVIYR
                     RLDDDFLDPLAFRKDSMLGVPGLLSAYRAGNVTLANAIGTGVADDKSIYPYVPEMVRF
                     YLGEESLLNNVPTWMLRKPDDLKYVLEHLPELVVKEVHGAGGYGMLVGPASTTKEIED
                     FRARIVADPGKYIAQPTLALSHCPTFVDDKKFGEVSPRHLDLRPFVLSGENIDMVPGG
                     LTRVALREGSLVVNSSQGGGTKDTWVLEH"
     gene            2190..3131
                     /locus_tag="PLD53_04160"
     CDS             2190..3131
                     /locus_tag="PLD53_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRC60132.1"
                     /translation="MLSRTADHLYWMSRFTERAENLARLLDVTHQMSLVPQSVAAENQ
                     NWGAILALNSLQTAFSKSYDEVNAENVLRFMVSDADNPASIYSCLRAARENAHAVRGT
                     LTAETWESCNATWIELREQSFEQILSTGVGEFFEWVKLRSAVMRGVTFGTMLQDDSLD
                     FIRLGGLLERGDNTARILDVKYHVLRTNGDEGTTDFYQWGALLRSLSAFEVYRKVYRD
                     VITPARVAELLMLRVDMPRSLHSCVDGIVRVLNRVANSSSVAETGRRAGMLHAQLHFA
                     RIDDVLSKGLHEYLTDFMDRIYDLGEGIGHDFLVPAS"
     gene            3176..3733
                     /locus_tag="PLD53_04165"
     CDS             3176..3733
                     /locus_tag="PLD53_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60133.1"
                     /translation="MSINVSVISTANSTRHFALNSVESIGHLLINLKRSSRIFTGNLL
                     IIGSGAQTEIFSTASIACVEMESENDLEVYLPRGQNLALTSLTTVEAIEPFEGGLESE
                     FFKVQIDFFFRGGHVIHTCVEGDPKLALDECLVNLTGVFEQPCLAYRLPQGGIGLMNP
                     LAMTRLVIIPGIPDLPKEAWVADDL"
     gene            complement(3747..4619)
                     /locus_tag="PLD53_04170"
     CDS             complement(3747..4619)
                     /locus_tag="PLD53_04170"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HRC60134.1"
                     /translation="MSAFLPYLKLMRPHQWVKSGFVFVGLFFGHAWHDAHIVHMVLLA
                     AAAFSLAASGIYIINDLADRERDREHPEKRFRPLAAGTVGVKQALVLAGVCVLSALTL
                     AWAASNSVLLVVCVYILLNVAYSMGLKRIALLDVFIISSGFMLRIVAGTVGVGIEPSH
                     WLLLCSLLLTLFLGFAKRRAEVNVMAGRGGHHRQVLDDYDPVLLDSLITICATGTIVS
                     YSLYTVSPETVAIHGTANLIYTVPFVIYGTFRYLFLLHRRARGGDPAATLLQDYHLLG
                     TFSAWFVTVMVLLR"
     gene            complement(4616..>4903)
                     /locus_tag="PLD53_04175"
     CDS             complement(4616..>4903)
                     /locus_tag="PLD53_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60135.1"
                     /translation="DWREQNGRNVLILRTSSPEGKDYSSYFRHVEFREFAVDGSRFYL
                     VLGQGFNYAVYHEKVLTRIRERFYRIPAWLPQRGCEFCERYFPDVKIIGQQ"
     assembly_gap    4904..5003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5004..5721)
                     /locus_tag="PLD53_04180"
     CDS             complement(<5004..5721)
                     /locus_tag="PLD53_04180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRC60136.1"
                     /translation="MLSLFPTRLSWVCLLSLLAFRLWFSAALPLTGDEAYFVFWGEHP
                     AGGFYDHPPMVGWWLTALLAVSRAEWFLRLPATLLPLVLAWGAWMMARPSGVERARVA
                     AWLVLMQPVNVWNVLITTDTPVILFSMLSMLAYFAALRCAGQPRRSLLWHVAAGALLG
                     LAFLGKYFAALLGVAYLVHVLFVRRDAARWPGFALLLVASLPAPIYNLWWNSGHCWVN
                     ILFNFLNRNAEAGLSFTNPAL"
     gene            complement(5729..6808)
                     /gene="aroG"
                     /locus_tag="PLD53_04185"
     CDS             complement(5729..6808)
                     /gene="aroG"
                     /locus_tag="PLD53_04185"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HRC60137.1"
                     /translation="MAVQSKPNTDDIRIREIKELMPPTHVFREFPVGVRAAQTTFGAR
                     QAIHRILHGADDRLLVVIGPCSIHDVDVAVDYAKRLNDEVPRFEKDLLIVMRVYFEKP
                     RTTVGWKGLINDPRLDNSFRINEGVRLARGLLLKINDMGLPCATEFLDTIMPQYTADL
                     ISWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNIRIAIDAIRAAQSPHHFLSVTK
                     AGMSAIVSTTGNEDCHVILRGGKEPNYDAAHVDAAAREIAAAALAARLMIDFSHANSS
                     KEYKRQLDVARNVAEQLAAGECRIVGVMVESHLVEGRQNLIPNKPLEYGKSITDACVG
                     WEDSVAILETLAQGVQSRRLIRADQ"
     gene            complement(6888..8501)
                     /locus_tag="PLD53_04190"
     CDS             complement(6888..8501)
                     /locus_tag="PLD53_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRC60138.1"
                     /translation="MKKHVFLVRLLTAVLGLLLLSGALVFAADNESKDAEAKEATLEF
                     NFRPIFVFRSTFANAAPTRRAERALERLDKLTAEQMQEPVELLPFDAKGIAAVAVRID
                     SLLMFVVAQSDIDPGSKTTLDEAAERVRTSLDEAIAALREQRRPEVLFKGIIFSLVAT
                     VLAFALAWGIARTRYWAAARLQVFIEQESRIPGLRLARYGWVFVQRAALLLTGALFLA
                     VAYLWLSYVLERFPVTAPLGRKLGSSLVSLLSLIGDGLVEAIPGMTTALVILFLTKAM
                     NDVMVNIFKAVREGRTELIGIHKETASATQRIASVIVWGFGIAIAYPFIPLSNSDAFK
                     GLSVMFGFMLTLGSAGIVNQLMSGLVLVYSRALSVGDFVDLGQTVGVVSEVGVLATKI
                     VNLRNEEVTVPNAVLIGNSITNYSRLAGKRGAMVSTKTTIGYDTPWRQVHAMLIAAAE
                     ATPGLRSTPPPFVYQRGLADFYVEYELFAYMDNPLQRVAVLSTLHANIQDQFNSHGVQ
                     IMSPHFVAQPQNNVVVPRENWNAPPAEPE"
     gene            complement(8588..10198)
                     /locus_tag="PLD53_04195"
     CDS             complement(8588..10198)
                     /locus_tag="PLD53_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRC60139.1"
                     /translation="MARVSNRSFLRLVLTALFGLSMVSGSGVMAADAPLKEAALEFNF
                     RQVIVFRASFLGTTPEVRVQRARARLNKLTPSQKVAPIEQDPFVFEGVTGIGLRIENV
                     VLFSVLAGDLDPDERLSLEAAAKRAAAALSEALRSESAQPPPDDLLKDIALTLLATVL
                     AIALLWMVRRATLFMVSHLQKVIERGEGTAKWRLARHGVSLVHRLSQLVMVLLWFGVA
                     DLWLTFVLDSFTRTRPLGDKLGSFIGDLLGDVGAGFLSALPGIATVLIILFMTMAVND
                     AISNFFVAVKKGHTRVRGLHKETLSATQRIVSILVWGFGFALAYPFIPLSSSDAFRGL
                     SVMFGFMLTLGSAGIVNQLMSGLVLVYSRALTIGDFVDIGGTVGVVSEVGVLSTKVID
                     SRNEEVTIPNAVLVGNPIRNYSRLSGERGSLVSTRVTIGYDTPWRQVHAMLITAAELT
                     PGLRKTPSPFVYQRDLADHYVEYELHAYMDQPLDRVPILSALHGNIQDQFNTYGVQIM
                     SPHFVSQPQDNVVVPTEKWYAAPAEPKP"
     gene            complement(10399..>11064)
                     /locus_tag="PLD53_04200"
     CDS             complement(10399..>11064)
                     /locus_tag="PLD53_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRC60140.1"
                     /translation="LHEAVGHGLEGDFNRKGSSTFSGRIGSRVASKGVTVVDDGTLPG
                     RRGSLNIDDEGNPTQRTVLIEDGILKGYMQDSHNAHLMGVAPTGNGRRESFAHLPLPR
                     MTNTTMLAGDKSPEEIIKSVKKGIYAVNFGGGQVDITNGKFVFSMSEAYMIEDGKVTY
                     PVKGATLIGNGPEAMNQVSMIGNDMRLDPGVGTCGKDGQSVPVGVGQPTLCIDGLTVG
                     GTA"
BASE COUNT         2499 a         3255 c         3056 g         2154 t
ORIGIN      
        1 tgcagcgctt ccatttcttc cttgtccttg tacagcgtcg aattggcata gacctcgacc
       61 ttgaccttac ccttggtcag ttcgcccgcc ttcttggcga agaaatccgc cgccttgccc
      121 ttgggcgtgt cgttggcgac gacgtgactg aacttgatga cgatcggttc ttgtgcggaa
      181 gcgagcggcg gaacagcaga aaggaagacg gctaccagac cgaaaagcag ttttgaagct
      241 ttcatattat gaatctcctc catctaacac gttaacgaga atcgggaaaa tcccgacacg
      301 cgacggtact atcacaaaaa cagcccaggc cggctattgt ggaaactacg taactggttt
      361 cggatgcgtg aatgcgggtt gtaccacatt gggcagcatg aaattgccta aggacgaggc
      421 gatggtctgt caaccgggaa gtcagttcct gtagcgacta ccccagctac agcgcaagca
      481 caccttctcg cagttgttga gatgctgaga gaagcgtcat tcttcataac catgctggga
      541 gctgtggccc attcgaacac tttggtttcg taacccacgc ctagccagcg aaatcttgct
      601 catttggtgc gcgcccgcac aactatggtg catggcgcga cgccagtgca cgcagaaagc
      661 ccgtattact gggtttcacg tttggcatgc tttctgctgg taactctcca aacccaccgc
      721 agcggcaaaa atcatgagca aagatttctt caacgaaatg tacgacgcag cgggcgccct
      781 gcggccgcac tatcgcgcct acgcagactg gctggcggct acacctcccg agcgaatcgc
      841 acgtaagcgg gccgaagccg atatcgcttt tcaccgcgtc ggtatcacat ttgctgtata
      901 tggcgaagag tcctcgcagg atcagggcaa agaacggctg attcccttcg acatcatccc
      961 gcgcatcatc ccggctgccg agtggtctgc gctgcaggcc ggtctgcgcc agcgggtaaa
     1021 aacactcaat gccttcctgc acgacatcta ccacaagcag gaaatacttc aggcagggct
     1081 catcccacgc gagcaagtgc ttaacaacgc gcagttccgc ccggagatga tgggggtcga
     1141 tgtcccggcc ggcatctacg cgcacattgc cggagtcgac ctcgtaaggg ccggcgaggg
     1201 cgagtattac gtcctggagg acaatctgcg cgtaccctcc ggcgtgtcct acatgatcga
     1261 agaccgcaag atgatgatgc ggcttttccc cgagcttttc tcgcggcaca agattgcgcc
     1321 agtacagcac tacccggaca tgcttttgga gaacctgcgt accgttgcac cggcgggcgt
     1381 cctcaatcca accgtcgtgc tgcttacgcc cggagcctac aacagcgcct atttcgaaca
     1441 caccttcctt gcccagcaga tgggcattga actggtcgag ggccgcgacc ttttcgtcag
     1501 ggatgagacg gtgttcatgc gcacaacgca aggcccgcag cgggtcgatg tgatctaccg
     1561 gcggcttgat gacgacttcc tcgatccgct cgccttccgc aaggactcca tgcttggcgt
     1621 tcccggtctc ctctcggcct atcgcgccgg gaatgtcacg ctggccaatg ccatcggcac
     1681 gggtgtcgcg gacgacaaat cgatctaccc gtatgtgccg gaaatggtgc gcttctatct
     1741 cggcgaggag tcgttactca acaacgttcc gacctggatg ctgcgcaaac ctgatgatct
     1801 gaaatacgtg cttgagcacc tgcctgaatt ggtggtcaag gaagtgcatg gtgcgggtgg
     1861 ttacggcatg cttgtcggcc cggcatcaac gacgaaagag atcgaggatt tccgggcgcg
     1921 cattgttgcc gacccgggga agtacatagc gcaaccgaca ctggctctgt cccactgccc
     1981 gacatttgtc gatgacaaga aattcggcga agtgtccccg cgccatctcg atctgcgtcc
     2041 ctttgttttg tccggcgaga acatcgacat ggttccgggt ggcctcacgc gcgtcgcgct
     2101 gcgcgaaggc tcgctggtcg tcaactcgtc gcagggcggt ggcacgaagg acacctgggt
     2161 actggaacac tgaggcgagg agaatcgata tgcttagccg aacagccgat catctttact
     2221 ggatgtcacg ctttaccgag cgtgccgaaa acctcgcccg tctgcttgac gtgactcacc
     2281 agatgtcgct cgtgccgcaa tcggtcgccg ccgagaacca gaactggggc gccatcctgg
     2341 cgctgaacag ccttcagacc gccttctcca agtcttacga cgaggtgaat gccgagaacg
     2401 ttctgcgttt catggtatcg gatgcagaca atccagcgtc gatctacagt tgcctgcgcg
     2461 ctgcgcgcga gaacgctcat gccgtacgcg gcacgctgac cgcagagaca tgggagagtt
     2521 gcaacgccac ctggatcgag ttgcgcgagc agagcttcga acagattcta tctaccggcg
     2581 ttggcgagtt tttcgaatgg gtcaagttgc gctccgccgt catgcgcggc gtgaccttcg
     2641 gcaccatgct gcaggacgat tcgctggact tcatccgtct cggtgggctg cttgaacgtg
     2701 gcgacaacac ggcacgcatt ctcgacgtga agtaccacgt gctgcgtacc aatggtgatg
     2761 agggcacgac agatttctac cagtggggtg cgctgttgcg ttcgctgtcg gccttcgagg
     2821 tctaccgcaa ggtctatcgc gatgtgatta caccggcgcg tgtcgccgag ttgctcatgc
     2881 tgcgcgtgga tatgccacgc tccctgcatt cctgcgtgga tggtatcgtg cgcgtgctca
     2941 accgggtcgc caacagcagc tcggtggcag aaaccggacg gcgcgccggc atgttgcacg
     3001 cgcaactgca cttcgcccgg atagacgatg tactgtccaa agggttgcac gagtatctga
     3061 ccgacttcat ggatcgcatc tacgatctcg gcgaaggcat tggacatgac ttcctcgtac
     3121 cggcaagctg aatgcggtaa tatgaattcc tgtctttcgt tgacgaggca tcgccatgtc
     3181 aatcaacgta tcggttattt ccacagccaa cagcacacgc catttcgcac tgaatagtgt
     3241 cgaatcgatc ggtcacctgc tcatcaacct gaagcgcagt tcgcgaattt tcaccgggaa
     3301 tctgcttatc atcggctcag gagcccagac cgagattttt tctaccgctt cgatagcctg
     3361 cgtcgaaatg gaatccgaaa acgacctcga ggtctacctc cctcgagggc agaacctggc
     3421 attgacctcg ctgacaaccg tcgaggctat tgagcccttc gaaggagggc tcgaaagcga
     3481 gttcttcaag gtgcagattg atttcttctt tcgcggcggt catgtcatcc atacctgtgt
     3541 cgagggtgac cccaagctgg cgcttgacga gtgcctggtc aatctgacag gggtttttga
     3601 gcaaccgtgc ctggcctatc ggctgcccca ggggggtatc ggcctgatga atccgctcgc
     3661 catgacgcga ctggtcatca ttcccgggat tccagatctg ccgaaagaag cctgggttgc
     3721 cgacgacctc tgacctgatt tccagctcaa cggagcaaca ccatgacggt gacaaaccag
     3781 gctgagaacg tgcccagcag gtgataatcc tgtagcagtg tggcggcagg gtcgccacca
     3841 cgagcgcgcc ggtgcaacag gaatagatag cggaatgtcc catagatgac aaagggaacc
     3901 gtgtagatca ggttcgctgt accgtgtatg gcgaccgttt cggggctgac cgtgtacaag
     3961 ctataactga caatcgtgcc tgttgcgcaa atagtgatca ggctgtcaag caacaccggg
     4021 tcgtaatcat cgagcacctg gcggtggtgt ccaccgcgtc cggccatcac attcacctcg
     4081 gcgcgccgtt tggcgaatcc aagaaagagc gtaagcagca ggctgcacag caacagccag
     4141 tgcgacggct cgatgccgac accaaccgtg cctgccacaa tgcgcagcat gaagcctgac
     4201 gaaatgatga atacgtcgag cagcgcaatg cgcttcaagc ccatcgagta cgccacattg
     4261 agcaggatat agacgcacac aacgagcagc accgaattcg aagcggccca tgccagcgtc
     4321 aatgccgaca acacgcagac acccgccagc accagcgcct gcttcacgcc cactgtcccc
     4381 gcagcaagcg gtcgaaagcg cttctccgga tgttcgcgat cacgttcgcg atctgccaga
     4441 tcgttgatga tatagatacc cgatgcggcc agtgaaaaag cggctgcagc caacagaacc
     4501 atatgaacga tatgcgcatc atgccaggcg tgaccaaaaa acaggccgac gaagacaaag
     4561 ccgctcttca cccattggtg cggacgcatg agtttgagat agggcagaaa tgcgctcatt
     4621 gttgacctat tattttgacg tccgggaaat agcgttcgca aaattcgcag ccgcgctgtg
     4681 gaagccatgc ggggattcgg tagaagcgtt cgcgtattcg ggtcagcact ttttcatgat
     4741 acacggcgta attgaatccc tgtcccagga ccagataaaa ccgtgagccg tcaactgcaa
     4801 attcgcgaaa ctcgacatgc ctgaaatagg acgagtagtc tttcccctca ggcgaactgg
     4861 ttctgaggat gagcacgttg cgtccattct gctcacgcca gtcnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnacaacgc cgggttggtg aacgaaaggc cggcctccgc
     5041 attccgattc aagaagttga aaagaatatt cacccagcag tggccgctgt tccaccacag
     5101 gttgtagatc ggtgccggca gggaggccac aagcagtagc gcgaatccgg gccagcgcgc
     5161 ggcatcacgc cggacgaaaa gcacatgcac cagatacgca acaccgagca gcgcggcaaa
     5221 gtatttgccc agaaaggcca gaccgagcag cgcaccggca gcaacatgcc agagcaatga
     5281 gcgccgcggt tgtccggcgc atcgcaaggc cgcaaaatag gccagcatcg aaagcatcga
     5341 aaacaggatg accggcgtat cggtggtaat cagaacattc catacattga ccggctgcat
     5401 cagaaccagc catgcggcga cccttgcgcg ctccacaccg ctcggacgcg ccatcatcca
     5461 ggcgccccag gccagaacta acggcagcag tgtggccggc agtcggagaa accattcagc
     5521 acgtgaaacg gcgagcaatg cagtcagcca ccagccgacc attggcgggt ggtcatagaa
     5581 ccctcctgcc ggatgctcac cccagaaaac gaagtaagcc tcatcgccag tcaatggcaa
     5641 agctgcggaa aaccacagac gaaatgccag aagagacagc aggcagaccc aggacaagcg
     5701 cgtcggaaaa aggctcaaca ctttgaagtt actgatcagc cctgatcagg cggcgagact
     5761 gaacaccctg tgccagggtt tccaggattg cgacagaatc ctcccatccg acgcaggcat
     5821 cggtaatgct tttaccatat tcgagcggct tgttcggtat cagattctgt cgcccttcga
     5881 caagatgaga ctcgaccatg acgcccacga tgcggcactc gcccgccgcg agttgctctg
     5941 cgacatttcg ggccacgtcc agttggcgct tgtactcctt gcttgagttg gcgtgcgaga
     6001 agtcaatcat caggcgtgca gccagtgcgg ccgcggcaat ctccctggcg gccgcatcga
     6061 catgcgccgc gtcatagttg ggctcttttc cgccgcgcag aatgacgtgg cagtcttcat
     6121 tgccggtcgt tgaaacgatc gccgacatac ccgctttcgt aacggacagg aaatggtgtg
     6181 gcgactgcgc ggcacgaatt gcatcgatgg cgatccggat attgccgtcg gtaccgttct
     6241 tgaatccgac cgggcacgac agacccgaag ccagttcgcg gtgcacctgc gattcggtag
     6301 tgcgcgcgcc gatggccccc caggaaatca gatccgccgt gtattgcggc atgatcgtgt
     6361 cgagaaactc ggttgcgcag ggaagcccca tgtcattgat ttttagcagc aggccgcgcg
     6421 ccagacgcac gccctcattg atgcgaaaac tgttgtcgag gcgcggatcg ttgatcagcc
     6481 ccttccaacc caccgtggtt cgcggcttct cgaaatagac gcgcatcaca atcagcagat
     6541 ccttctcgaa acgcggcact tcgtcgttca ggcgtttggc gtaatcgacc gcgacgtcga
     6601 catcgtgaat cgagcacgga ccaatcacca ccagcagtcg atcatcggca ccatgcagta
     6661 tgcgatgaat cgcctgacgt gcgccaaaag tggtctgtgc ggcccgcacc ccgacaggga
     6721 actcacggaa aacgtgagtt ggcggcatca gttccttgat ctctctgata cgaatgtcgt
     6781 cggtattcgg cttgctttgc acggccatgt agcaccctct gatagacccg caggtccaaa
     6841 gcggtcgatt ttacgcgaag acctctattg gcgccctgct cgtgcgctca ctccggctca
     6901 gcgggcgggg cattccagtt ctcgcgcggg accaccacgt tattctgcgg ctgcgcgaca
     6961 aaatgcggcg acatgatctg aacgccgtgg gaattgaact gatcctgaat attggcatgc
     7021 aaggttgaca gcaccgcgac gcgctgaaga gggttgtcca tgtaggcgaa aagctcatac
     7081 tcgacgtaaa aatccgccag tccgcgctga tagacgaagg ggggcggagt gctgcgcaag
     7141 cctggtgtcg cttcagccgc ggcaatcagc atggcatgca cctgtcgcca tggcgtatcg
     7201 tagccaattg tcgtcttggt cgacaccatg gcgccgcgtt tgccggccag gcgtgagtaa
     7261 ttggtaattg aattgccgat cagcaccgca ttgggcacgg tcacttcctc gttgcgcaaa
     7321 ttgactatct tggtcgccag cacgcccact tcgctgacca cgcccacggt ttggcccaga
     7381 tcgacgaaat cgcccaccga aagcgcgcgc gagtaaacca ggaccaggcc gctcatcagc
     7441 tgattgacaa tccccgccga gccaagtgtc agcatgaatc cgaacatgac cgacaaaccc
     7501 ttgaaggcat cgctgttgga gagcgggata aagggatagg caatggcgat gccaaaaccc
     7561 cagacgatga cgctggcaat ccgctgcgtg gcactcgccg tctctttgtg aatgccaatg
     7621 agttcggtac gcccctcacg taccgccttg aagatattga ccatcacgtc gttcatggcc
     7681 ttggtcaaaa agagaatgac cagcgccgtt gtcatgccgg ggatggcttc gaccaggccg
     7741 tcaccgatca aggacaacaa actgaccagc gaactgccca gcttgcgccc cagcggcgcg
     7801 gtcaccggaa aacgctccag cacataagat agccaaaggt aagctaccgc caggaacaaa
     7861 gccccggtca gcaacagtgc cgcgcgctgg acaaacaccc agccgtagcg cgccagacgc
     7921 aaacccggta tgcgtgactc ctgctcaatg aacacttgca gacgcgcggc cgcccagtac
     7981 ctggtacgtg caattcccca ggccagggcg aacgccaaca cggtggccac cagcgaaaat
     8041 atgattccct tgaacagcac ctcggggcgt ctttgctcgc gcaacgcagc aatagcctcg
     8101 tcaagggacg ttctgacccg ttcggccgcc tcatcgagcg ttgttttgga tccggggtca
     8161 atatcggact gagcgacaac gaacatcaat agcgaatcaa tacgcacagc cacggccgca
     8221 atccccttgg catcaaacgg aaggagctcg accggttcct gcatctgttc tgcagtcagt
     8281 ttatccaggc gctccagtgc ccgctcggcg cgacgggttg gcgccgcatt ggcgaaagtt
     8341 gatcgaaaaa caaagatggg ccggaaattg aactcaagag tcgcttcctt tgcctccgcg
     8401 tccttcgact cgttgtctgc cgcaaaaacc aaagcgccag aaagaagcaa tagccccaat
     8461 accgcggtca gcaagcgcac aaggaagacg tgtttcttca tgatcaacac ccgttagagt
     8521 tgtctcaaag cgagatacgg attatagctt tgcagctcgt cgaggccgcg tggaaactgc
     8581 attcagttta tggctttggc tcagccggag ccgcgtacca cttctccgtc ggcacaacca
     8641 cgttatcttg cggttgcgaa acgaaatgtg gcgacatgat ctgcacgccg taggtattga
     8701 actggtcctg aatattgccg tgcaaggccg acaggatggg cacgcggtcg agcggttgat
     8761 ccatataggc atgaagttcg tactcgacgt agtgatccgc cagatcccgc tgatatacga
     8821 aagggctcgg cgtcttgcgc aaacccggcg tcagttcggc agcggtaatc agcatggcat
     8881 gtacctgacg ccacggcgtg tcgtagccaa tcgtcacccg ggtagacacc agcgaaccac
     8941 gctcaccgga caatcgtgaa taattgcgga tcgggttgcc aaccagcaca gcgttgggaa
     9001 tggtcacttc ctcattgcgt gagtcgatga ccttggtcga caacacaccc acctcgctga
     9061 ccactcccac ggtgccgccg atatcgacaa aatcaccgat cgtcagtgcc cgcgaataga
     9121 ccagcaccaa cccgctcatc aactggttga cgataccggc cgagccgagc gtgagcatga
     9181 agccgaacat caccgacagc ccccggaagg catcgctgct ggataaggga ataaaaggat
     9241 aggcaagggc aaacccgaac ccccagacca gaatgctgac aatgcgctgc gtggcgctga
     9301 gggtttcctt gtgcaaaccg cgtactcttg tgtgtccctt cttgacggcc acaaagaaat
     9361 tggaaatggc atcattcacg gccatggtca tgaaaaggat gatcaatacc gtcgctatcc
     9421 cagggagtgc gctcaggaaa cctgccccca cgtcacccag caagtcgcca atgaaggagc
     9481 ccagtttgtc cccgagcggt ctggtccggg tgaaactatc gagaacaaag gtcaaccaga
     9541 gatcggcgac accaaaccac agcaagacca tcaccagttg agaaagccga tgcacgagcg
     9601 acacgccatg gcgcgccagg cgccacttag cagtgccctc tccccgctcg atgacctttt
     9661 gcaggtggct gaccataaag agagtggctc gccgaaccat ccagagcaga gcaattgcca
     9721 acacggtggc gaggagcgtg agcgcaatgt ctttgagcag atcatccgga ggcggttgtg
     9781 cactttcaga acgaagcgct tcagaaagcg cagcggcagc acgcttggct gcggcttcca
     9841 gcgacagcct ttcatcgggg tcgagatcgc cagccagtac gctgaaaaga acgacgtttt
     9901 ctattcgtaa accaatgccc gtcacacctt caaaaacaaa cggatcctgc tcgatcggcg
     9961 caaccttctg gctgggcgtc agtttgttca aacgtgcccg cgcgcgctgt acgcgaactt
    10021 ccggcgtggt gccgagaaac gaggctcgga agactatcac ctggcggaag ttgaactcga
    10081 gcgccgcctc cttcaagggc gcgtcggcgg ccatcacgcc agagccgctg accattgaca
    10141 agccgaacaa ggccgtcaga acaaggcgaa gaaacgaacg attggatact cgcgccatgg
    10201 ttaattccga aaaggtatgg gttgaaaaat gccgtggatt ataagcgtct cgcctgcaaa
    10261 caacgccttg ccatccggcg tgactgcctg aggaagacgg ccgcattcgt agcgtatccg
    10321 aagacacccg cattcagcag gtaattgggc aagctgctcg aagactgccg aatgacgcgc
    10381 ttctctggcc gagaggtatt acgccgtacc gcccacggtc aggccgtcaa tgcacagggt
    10441 tggctgaccc acacccaccg gaacgctttg tccatccttg ccacaggtgc cgacgccggg
    10501 atcgagacgc atgtcgttgc cgatcatcga cacctgattc atcgcctcgg gcccgttgcc
    10561 gatcagcgtt gcgcccttga ccgggtaagt gaccttgccg tcctcgatca tgtaggcctc
    10621 ggacatcgag aaaacgaact ttccattggt gatgtcgacc tggccaccgc cgaaatttac
    10681 cgcgtaaatg cccttcttca ccgacttgat aatttcttcg ggactcttgt cacccgccag
    10741 catcgtggtg ttggtcatgc gtggcagcgg tagatgcgca aaggattcgc gacgaccatt
    10801 gccggtcggc gccactccca taaggtgcgc gttgtggctg tcctgcatgt aacccttgag
    10861 aatgccgtct tcgatcagca ccgtacgctg agtggggttg ccttcatcgt caatgttgag
    10921 cgatccgcga cggccgggaa gcgtaccgtc atcgaccacg gtaacgcctt tcgatgccac
    10981 gcgactaccg atccggccgg agaacgtcga gctgcccttg cggttgaaat cgccttccag
    11041 accatggcct accgcttcat gcaa
//
LOCUS       DAORPM010000055        11006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1331_length_11006_cov_9.920738, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000055 DAORPM010000000
VERSION     DAORPM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 11006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11006
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1331_length_11006_cov_9.920738"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..920
                     /gene="gloB"
                     /locus_tag="PLD53_04205"
     CDS             159..920
                     /gene="gloB"
                     /locus_tag="PLD53_04205"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HRC60141.1"
                     /translation="MFDVIRIPAFKDNYIWLLRKGAAAAVVDPGDARPVLEVLERESL
                     TLASILVTHHHADHQGGVAGLLARHPAEVFGPASESITGITRPLSGGETIRLPALGVE
                     FRVLAVPGHTLGHLAYHGSGCLFCGDTMFGAGCGRLFEGTPAQMYDSLMRLASMPDQT
                     AVYCAHEYTESNLRFAQTVEPGNGQLRQRVDEVRVARADGRATVPSTIALEKATNPFL
                     RCGEPEVIASVQRRESKAGNAVEVFAVLREWKNRF"
     gene            complement(1009..1965)
                     /locus_tag="PLD53_04210"
     CDS             complement(1009..1965)
                     /locus_tag="PLD53_04210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRC60142.1"
                     /translation="MKTLVIEDSTSSLKLLCSYIEKIGISTIPAENGATGIDLFLSEH
                     PDLILLDIILPDLDGYEVARQIRLLEPPGEWTPIIFLSSMNKDKDIEMGIAAGGDDYL
                     LKPISEVVLAAKIRAMQRIIQMRQSLLVLTRKLDSANQELKRLTSLDGLTGIPNRRHF
                     DEVLAREWRRAMRQGEELSILMCDIDFFKNFNDTFGHQKGDECLRQVANALADSLDRG
                     GDLLARYGGEEFIAVLPGTTLEGASFIAAQMRQSICQLNISHPETPFGKVTASFGVAS
                     AVAMPETDPLKIVGAADTAMYKAKKAGRNRVFRTTSLDPQED"
     gene            2191..5562
                     /locus_tag="PLD53_04215"
     CDS             2191..5562
                     /locus_tag="PLD53_04215"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HRC60143.1"
                     /translation="MRCLIAVVIYGVLGFFVLPPLIKPVLVEKLGQALHRPVAIESLS
                     INPFALSVTLDGVAIQEREGAEIFAGFDSLYLNLESSSLFRGGPVLSEIRLAKPKLRV
                     VRLTDKQYNFSDLFDEFMAKPKSDGPTPPFSLNNIQITDGAVEFDDRPVDEKHVVSDI
                     NLSLPFISSMAYATDSVVEPSFSASVNGAPLAINGKSKPFAHSLETELALDLGNLQLA
                     KYVDYIPYDLPIKVLSGVLDSNLNLVFHQQEGQPASLLLSGTAAIRGLEVNNSSDAPL
                     VSVKQLDLGIGSSDLLGGRFVIDRIAVDSPELHARVSREGTINWIEFFRKKLDAETSA
                     PQAVEKPEPATPIEWSLGEVKVAGGALHWLDESNEKPFEASIEAIELELMKLDSIGSV
                     PGTFDLSWRMTAEEWLKIDSFAIKGGQLNLAKREVLAGNVLARGVRALVRRKADGTID
                     WLKPPALRAAEALQEDNSAPWKITVAKYVGEDIAARFEDQAVSPAAVQSVENLGFEIE
                     NLSTVPEQTAKLAVHFQINGKGVLAVDGTAQTFPLNADLNLDIKSLELLPLQPYFTEK
                     LNIAVTRGQVSANGKVQLRQGSAGKTAGPSSLTGGFSGLATIADFNAVDKLNSADFLR
                     WKSLYFGKVDVRLGPDSISVGEVALSDFFARIIVSPDGKLNLMQIVRKDGASAVAVVP
                     QPSDPAASPANETSDGKAVAPVAATSEERKPLLPIKIGKITLQGGQVRFSDNFVKPNY
                     SANLKQIGGHVTGLSSEQDSIATLELRGKYDNVSPLNVSARINPLSVKPYLDLQADIK
                     GIELTSLSSYSSKYAGYMIEKGKLSLFVKYKIKNDQLEAENRVLLDQLTFGEAVESPD
                     ATSLPVTLAVALLKNSKGEIDLNLPISGSLNDPQFSIGGIVAEIIVNAILKAVTSPFA
                     LLGSMFGGGEELSNVEFDPGRASISPAAEKRLDSLAKALIEKAELKLEVEGRVDPESD
                     REGLKHARLERKVKALKREEMKQGGSDSDSTESANADGSARSIEVSSAEYPELLERVY
                     RAEKFTKPRNMIGMVKSLPVEEMEKLILANSVVDDDDLRKLGDRRSKAVRDWLVAHEI
                     PPERLFLLPSKVGEEKAKSDSAEQAKRSRVDFSLK"
     gene            5656..7002
                     /gene="rmuC"
                     /locus_tag="PLD53_04220"
     CDS             5656..7002
                     /gene="rmuC"
                     /locus_tag="PLD53_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRC60144.1"
                     /translation="MNEYLIYGLAAIGVLVFLMQIVLLWRSSRKDDAFDPFFRSLGDG
                     LERVERELRHEMASGRQEAASAARGDREEQSQALDRLTKTLAAQVGQLGTLQGQQLES
                     FAQQLARLTQSNEQRFEQLRLSVETRLVAMQADNASKLEEMRKTVDEKLHATLEQRLG
                     DSFRLVSERLEQVHKGLGEMQTLAAGVGDLKKVLTNVKTRGTWGEVQLEALLDQVLTA
                     EQYRKNVATRPNSSERVEFAICLPGREMGEDDKRPVWLPIDAKFPMEDYQRLIEAQER
                     ADPLAVELAVKALELRLRDEAKKIRDKYVEPPYTTDFAILYLPTEGLYAEALRRPGLA
                     DGLQRDFRISIAGPTTLAALLNSLQMGFRTLAIEKRSSEVWGVLGAIKTEFGKFGEVL
                     EITRRKLEQATRSIESASVRTRQIERKLKGVEALPMLEAQTRLGELEEIGQAEEDV"
     gene            7089..7409
                     /locus_tag="PLD53_04225"
     CDS             7089..7409
                     /locus_tag="PLD53_04225"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HRC60145.1"
                     /translation="MHPEFDNFLRRLELSCWGVITAYNPGRVLREEDNAERQIRLLER
                     IEELGWPHFPACNIADDGLWPVEPGYLLLQVREMAVCHLAAEFDQSACVCGDTGAAPR
                     LVWI"
     gene            7490..9922
                     /locus_tag="PLD53_04230"
     CDS             7490..9922
                     /locus_tag="PLD53_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRC60146.1"
                     /translation="MSKKIGRFEIIRELGRGAQSVVYLARDPHLQREVAIKTLHFSRP
                     DPQKNEQLLSEARMVSQLRHPNIVPIFEADEEQGDLYLVFEYVPGKNLGEFLAASGRL
                     TPVKAIAILHPVLDAIAHAHAAGIIHRDLKPNNILIGVDGLPRVMDFGIAARVETHSN
                     DGDTFMGTPSYMAPEYIAHRESSERTDVFSAGLVLYEMLTGQRAVQGDDVYGIMHQVA
                     NADIKVPATAGVELDDQLASILYKALARDPQQRFASINDMREALDGYLEPEEPVASAD
                     TKQSTIDFLLRRMRHKSDFPALSESVGTINKIAGSDKESISNLSNSILKDFSLTNKIL
                     RLVNSAYYRQVGGGSISTISRAVLVLGFDAVRNIAITVMLFEHLQNKSNAKQLKEEFL
                     RANLASILAKDISEKSSARDVEQVFICSMFHNLGRLLSQFYFPDESTEIKRMIEQKLC
                     SEEAAAVQTLGISYEDLGIGIARTWGFPGLIVNSMRKLPAGNVRKASTVEERMRVLSA
                     FANDLCGLIADVEPEQRQKELRKVMSRFGENISLNEQALRETMEKSMQEVVLFAGIIG
                     VNLKQSEFGRQMKTWRAKSGAAEGAEEEKRIAAEGAETVLSDVVPAGAPGSPVIAGEE
                     IEAGEEGVSGAAAQIGNSEVILMAGIQDISNTLVEDFKLNDVLRIILETMYRAKGFKR
                     VILCVRDARNNSMLGRFGFGPDAPEIAKRFNFSLTFSPDIFHAALSKGVDILISDTKD
                     AKISARIPEWFSKGVNAGTFVIFPLSIKNNPVAMIYADCDLAGEIVITEKELSLMRTL
                     RNQAVLAIKQSS"
     gene            complement(9948..10742)
                     /locus_tag="PLD53_04235"
     CDS             complement(9948..10742)
                     /locus_tag="PLD53_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRC60147.1"
                     /translation="MRSLAVLTALFLAMLVAGCGLFPEQQDETAGWSANKLYTEAKEA
                     MNDGAYEKSIKYFEKLESRYPYGRYAQQAQIDIAYAYWKDNEPALAVAACDRFIKLHP
                     NHPNVDYVYYLRGLVNFNEDLGILGQISQQDMTERDPKGARESFDSFRELVTRFPDSK
                     YTPDAILRMKYLVNALASLEVHVARYYMKRGAYIAAANRAQFALKHYPEAAATEEALF
                     IMVKAYDLMGMNDLRDDAERVMRKNYPNSVYYTRGLERKEPWWKLW"
     gene            10720..>11006
                     /locus_tag="PLD53_04240"
     CDS             10720..>11006
                     /locus_tag="PLD53_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRC60148.1"
                     /translation="MRTAKLRMMKSPDRETEVDSHKEKNGKDAERADYSANPRLALSV
                     PADCGGQRLDQVLARLRPQHSRSRMQIWIRQGRVTVGGVTVSEPRQKLWGG"
BASE COUNT         2452 a         2816 c         3281 g         2457 t
ORIGIN      
        1 ggcagcccga gctgcagcgc gttgtaaccg aacacatcag cgaccagcgc gtcaattctg
       61 ggttgctccc aggacatgat gtagcgtcct tgtggcgtgt gcagccaagc attgatcccg
      121 ggaattgaca ttttccgcag ttcttccaca atcagaccat gtttgacgtc atcaggattc
      181 cggctttcaa ggataactac atctggctgt tgcgcaaggg tgctgcggct gcggtggttg
      241 atcccggcga tgcccgaccc gttctcgagg tgcttgaacg ggagagcctg acgctggctt
      301 ccattctggt tacacatcac cacgcggatc accagggcgg ggtagccggt ttgctggcac
      361 gtcaccccgc cgaagtattt ggtccggcgt cggagtctat cacaggcatc acgaggccat
      421 taagtggcgg tgagacgatc cgcctgcccg ctctgggcgt cgagtttcga gttctcgccg
      481 ttccgggcca tacgctcggg cacctcgcgt atcatggttc cggctgtttg ttctgcggcg
      541 atacgatgtt cggcgcgggc tgtggacgcc tgtttgaagg tacaccggca cagatgtacg
      601 attcattgat gcgactggcg tcgatgcccg atcagacggc cgtgtattgc gcccacgagt
      661 acacggagtc caatttgcgg tttgcgcaga ccgttgagcc cggcaacggc caattgcggc
      721 aacgggtcga tgaagtcagg gtggcgcggg cggatgggcg ggcgaccgta ccctcgacta
      781 tcgcgcttga gaaagcgacg aatccgtttt tgcgttgtgg cgaacccgag gtcattgctt
      841 ctgtccagcg cagggaatcc aaagccggaa atgcagtgga agtatttgca gtgctgcgtg
      901 agtggaagaa tcgcttctga gggactgcgc atgcggctga tggtgccgca gctgagtgcc
      961 cggctgatct cttgtgcctt attcagcaga gcacgtattc aagcgcgttc agtcttcctg
     1021 aggatcaagc gatgtggttc ggaaaacgcg atttcgacca gcttttttag ccttgtacat
     1081 cgccgtatcg gccgcgccaa caattttcag tgggtccgtc tcgggcatgg ccacggctga
     1141 agcaacacca aagctcgctg tgactttccc aaacggtgtt tcggggtggg aaatattcaa
     1201 ttggcagatg gactgtcgca tttgcgccgc aataaacgag gcgccctcaa gagttgttcc
     1261 cggcagaacg gcaatgaact cctctccgcc gtagcgggcg aggaggtcgc cgccacgatc
     1321 cagtgagtca gccaaagcgt tggcaacttg acgcagacat tcgtcaccct tctggtggcc
     1381 aaaggtgtca ttgaagttct tgaaaaaatc gatatcgcac atgaggatcg acaattcctc
     1441 gccctgacgc atcgcgcgtc gccattctct agccagtact tcgtcaaaat gacggcgatt
     1501 gggaatgccg gtcaggccat cgagcgaggt cagtcgcttc agctcctgat tggccgaatc
     1561 gagtttgcgg gtaagtacaa gcagcgattg gcgcatctgg atgatgcgct gcatggcgcg
     1621 aatcttggcc gccaggacaa cttcgctgat cggcttgagg agatagtcgt cgccgcccgc
     1681 agcgataccc atttcgatgt ccttgtcctt attcatggaa ctcaggaaga taatcggcgt
     1741 ccattcaccc ggaggctcca gaagacgaat ctgacgagct acttcatagc catcgaggtc
     1801 ggggaggatg atgtcaagca gtatcagatc cggatgttcg ctcagaaaga ggtcaatgcc
     1861 cgtcgcgccg ttctcggccg gaatggttga aatgcctatt ttctcgatgt aactgcaaag
     1921 aagtttcaga ctgcttgtgc tgtcttcaat gacgagggtc ttcatgcttg tcccgaaaga
     1981 gaattgttag atcatgcgct attctagccc ggttggcaca atagtggttt cggctgagcc
     2041 ggccgggttc tgttgccggt ccgaaagcgc ggtgttcgtg ataaattcag ctttccggca
     2101 agaaattcac ggctgttcat catgatcaac aatcacgtca cttcgaaact tcagaccgtt
     2161 cttcattcgc agaggacgaa gaagattgct ttgcgatgtt tgatcgcggt cgtcatttac
     2221 ggagtgcttg gtttctttgt cttgcccccg ctgatcaagc ctgtgctagt tgagaaactg
     2281 ggccaggcct tgcatcggcc ggtagccatc gaaagcctga gcatcaatcc ttttgctctg
     2341 tccgtgacac tggacggtgt ggcaattcag gaacgggagg gcgccgagat ctttgccggt
     2401 ttcgatagcc tgtatctgaa ccttgagtcc agttccttgt tccggggtgg tccggtacta
     2461 agcgagattc gtctggcgaa acccaagctc agggtcgtgc gcttgacgga caagcaatac
     2521 aacttctccg acctgtttga cgagttcatg gccaagccga agagcgacgg tccgacaccg
     2581 cctttttctc tcaacaacat tcagattacg gatggcgcag tcgaattcga cgatcgcccg
     2641 gtggacgaaa agcatgttgt cagtgacatc aatctgagct tgcccttcat ttcaagcatg
     2701 gcgtatgcga ctgacagtgt tgtagagccg tcgttttcgg cgagtgtcaa tggggcgccg
     2761 ctggcgatca atggcaagag caagccgttc gcccattcgc tggaaaccga gctggcgctt
     2821 gatcttggca acctgcaatt ggccaagtat gtcgattata ttccgtatga tttgccgatc
     2881 aaggtcttgt ccggtgttct ggacagcaat ctgaatctcg tgttccatca gcaggagggt
     2941 caacctgcaa gcttgcttct gtccggtacg gcagccataa gaggccttga ggtcaataac
     3001 tcgtccgatg cgcccctggt atcggtcaag caactcgatc tgggcatcgg ttcgtccgat
     3061 ctcttgggcg gccggtttgt cattgatcgt atcgccgtcg attcgcctga acttcatgcg
     3121 cgtgtcagcc gcgaaggcac gatcaactgg atcgagtttt tccgcaagaa gctcgatgct
     3181 gagacatcgg caccgcaggc ggttgaaaag ccggagcctg ccactccgat cgaatggtcg
     3241 ctcggggaag tcaaggtggc aggcggtgca ttgcactggc tcgatgagtc gaatgagaaa
     3301 ccgttcgagg cgagcatcga agccattgaa ttggagctga tgaaactcga tagcatagga
     3361 agtgttccag gcacgttcga tctttcctgg cgaatgaccg cagaggagtg gttaaagatc
     3421 gattccttcg cgatcaaggg tgggcagctc aatctggcca aacgcgaggt gctggcaggg
     3481 aacgtgctgg cgcgcggggt gcgcgccctg gttcgacgca aggccgatgg cacgatcgat
     3541 tggctcaagc caccggcact gcgcgctgct gaagcgttgc aagaagacaa ttcggcgccg
     3601 tggaagatca cggtggcgaa gtatgtgggc gaagatatcg ccgcgcgatt cgaagaccag
     3661 gcagtttccc cggcggcggt gcagagtgtc gagaacctgg gctttgagat tgaaaacctg
     3721 tcgactgtgc ctgaacagac agcgaaactt gcagtgcatt tccagatcaa tggcaaaggt
     3781 gtgcttgccg tcgatgggac cgcacaaact ttccccttaa acgctgacct gaatcttgac
     3841 atcaagtcgc ttgagttgct gccgctgcaa ccctatttca cagagaagct gaacattgcg
     3901 gtgacccgag gacaggtgag cgcgaatggc aaagtgcaat tgcggcaggg atctgcgggc
     3961 aaaacggctg gcccaagctc gctgaccggg ggattttccg gtctggcgac gattgctgat
     4021 ttcaacgcgg tggataagct gaactctgca gatttcttgc gctggaagtc gctctacttc
     4081 gggaaagtag acgtgcgtct tggtcctgat tcgatatccg tcggcgaggt ggcgctatct
     4141 gacttctttg cccgtattat cgtcagcccg gacggaaagc tcaacttgat gcagattgtt
     4201 cgcaaggatg gagcttcggc ggtggcggta gtccctcagc cgtctgatcc ggccgcgtcg
     4261 ccagcgaacg aaacatcgga cggcaaggcg gttgcgccag tcgcggcgac atcggaagaa
     4321 cgcaagccgt tgctgccgat caagatcgga aaaatcactt tgcagggcgg ccaggtcaga
     4381 ttcagcgaca acttcgtcaa gccgaattat tcggcgaatc tgaagcagat cggtggccat
     4441 gtgactggct tgtcgtcaga gcaggactcc attgccactc ttgagttgcg tggcaagtac
     4501 gacaatgtat cgccgctcaa tgtgagcgca cggatcaatc cactttcagt taagccctat
     4561 cttgatctgc aggcggatat caagggcatc gagttgactt ccctgtcctc ctattccagc
     4621 aaatacgccg ggtatatgat cgaaaaaggc aaactttcat tgttcgtcaa gtacaaaatc
     4681 aagaatgatc aacttgaagc cgagaacagg gttctactcg atcaattgac cttcggcgaa
     4741 gctgttgaaa gcccggatgc gacctcactg cccgtgacgc tggcagttgc actgctaaag
     4801 aatagcaaag gcgagattga cctgaatctg cccatttcgg gttccctgaa tgatccacag
     4861 ttcagtatcg gaggaatagt cgccgagatt attgtcaatg ccattctcaa agcggtgact
     4921 tcgccctttg ccttgctcgg ttcaatgttt ggtggcggcg aggaactgtc aaacgtagag
     4981 tttgacccgg ggcgagcaag catttctcct gcggcggaga aacggcttga tagtctggcc
     5041 aaggcgctga tcgagaaagc ggagctcaag ctagaggtcg aagggcgagt tgatcccgag
     5101 tcggaccgtg agggcttgaa gcacgcacgt ctggagcgca aggtcaaggc cttgaagcgg
     5161 gaagagatga aacaaggcgg cagcgatagt gattcgactg aatcggctaa cgcagacggt
     5221 tcggcgcgct cgatcgaggt cagttctgcc gagtatcccg agctgcttga acgtgtttac
     5281 cgcgcagaaa aattcaccaa gccgcgcaat atgatcggca tggtaaagag tctgccggtt
     5341 gaggaaatgg agaaattgat attggccaat tccgtcgttg acgacgatga cttgcgcaag
     5401 ctgggggatc gtcgttcgaa ggccgtgcgt gactggttgg tggcccatga gattcctcct
     5461 gagcggctat tcctgctgcc aagcaaggtt ggcgaggaga aggccaagtc cgactctgca
     5521 gaacaagcaa agcgtagtcg agttgatttc tcgctgaagt gagatcgaaa ccctgaactt
     5581 tcttcgactg ggttctcggt gtttgtcgcg cctgaaaatt cgtgtcatcc cgaaagcaat
     5641 ttgtgaaaaa tgggtatgaa tgagtatttg atctatgggc tggcggcgat aggcgtcttg
     5701 gtctttctga tgcagatagt cttgttgtgg cgttccagcc gcaaggacga tgcgttcgat
     5761 ccgttcttcc gctcattggg cgacggcctg gagcgggtcg agcgcgaatt gcgccatgaa
     5821 atggcgagcg ggcgacaaga ggcggcaagc gccgccaggg gagaccgcga agagcagtcg
     5881 caggcgctgg atcgattgac caagacactg gctgcccagg taggccagct tgggaccttg
     5941 cagggacagc aactcgagtc gtttgcgcag cagctggcac gtctgacgca aagcaatgag
     6001 cagcgtttcg agcaattgag gctatcggtt gaaactcgcc tcgttgccat gcaggccgat
     6061 aacgcgagca agcttgagga gatgcgcaag accgttgatg aaaagctgca cgcgacgctc
     6121 gaacagcgcc tcggcgactc gttcagactg gtcagcgagc gtctggaaca ggtgcataag
     6181 gggcttggcg aaatgcagac actggctgcc ggcgtcggcg acttgaagaa ggtactgacc
     6241 aatgtcaaaa cgcgaggcac ctggggtgaa gtgcaattgg aggccttgct cgatcaggtg
     6301 ctgaccgccg aacagtaccg gaagaatgtc gcaacgcgac caaacagtag tgagcgcgtt
     6361 gaattcgcca tttgcctgcc cggacgcgag atgggcgagg acgataaacg cccggtctgg
     6421 ttgccgatcg acgcgaaatt cccgatggag gactatcagc gcctgatcga agcgcaggag
     6481 cgggccgatc cgctggccgt ggagttggcg gtcaaggctc ttgaattgcg tctgcgcgat
     6541 gaggcgaaga aaatccgcga caagtatgta gagccaccgt acaccactga tttcgcaata
     6601 ctctatttgc ccaccgaggg gttgtatgcc gaagccttgc gtcgtcctgg cctggcggat
     6661 ggtttgcagc gtgattttcg catcagcatt gccggcccga ccaccctggc ggctttgctc
     6721 aatagtctgc agatgggctt tcgaaccctg gccatcgaaa aacgttcttc ggaggtctgg
     6781 ggcgtgctcg gtgccatcaa gaccgaattc ggcaagtttg gtgaagtgct tgagattacg
     6841 cgcaggaagc ttgagcaggc cacccgaagt atcgaatcgg ccagcgtgcg gacgcggcag
     6901 atcgagcgca aattgaaagg cgtcgaggcg ttgcccatgc ttgaagcgca gaccagactg
     6961 ggtgagcttg aggagatcgg gcaagctgag gaagacgttt gaaaacaaac gttcttgatg
     7021 cttcatttcg ggcgacaagc taccaggtag aaacgtccga tggtgtattc agattgcgaa
     7081 ttggggaaat gcatcctgaa ttcgacaact tcttgcgccg actggaactg tcttgctggg
     7141 gcgtgattac cgcatacaac cctggacgcg tgctccgtga agaagataat gctgagcgtc
     7201 agattcgttt gctcgaaaga atcgaagaat tgggctggcc gcatttcccg gcctgcaata
     7261 tcgccgatga tggtctttgg ccggtggagc cgggctatct ccttctgcaa gtacgcgaaa
     7321 tggcagtatg ccatctggct gcggagtttg atcagtctgc ctgtgtctgt ggcgataccg
     7381 gagctgcccc gcgtcttgtc tggatttgat tttcctgaca cagcgaggtt ttcacaccac
     7441 cgggccttga tgcgggtaat atcaatgcgc catagaaatg acgaacatca tgagcaagaa
     7501 gatagggcga tttgaaataa tccgtgaatt gggacggggc gcacaaagcg ttgtgtatct
     7561 ggcgcgcgac ccgcacctgc agcgagaggt cgcgatcaag acactgcatt tttcgcgccc
     7621 cgatccgcag aagaacgagc agttgctttc tgaagcgcgc atggtcagcc agttgcgcca
     7681 ccccaacatc gttcccattt ttgaagcgga tgaagagcag ggggatctgt atctcgtctt
     7741 cgagtatgtt cccggcaaaa atcttggcga attcctggca gcaagcggtc gcctgacgcc
     7801 cgtgaaagcc attgcgattc tgcatcccgt gctcgacgcg atcgcccatg ctcatgcagc
     7861 aggcatcatc catcgcgatc tcaagccgaa caacatactg attggcgtcg atggcctgcc
     7921 gcgcgtaatg gactttggca ttgcggcacg tgtcgagacg cattcgaacg acggggatac
     7981 gttcatggga acgccgtctt atatggcgcc cgagtacatc gcccatcggg aaagcagtga
     8041 acgaaccgat gtgttttcag ccggcttggt gctgtatgaa atgctgaccg ggcaacgcgc
     8101 ggtgcaaggc gacgatgtct atggaatcat gcatcaggtg gccaatgcag atatcaaggt
     8161 gccggcgact gctggcgtcg aactcgatga tcaactggcc agcatcctgt acaaggcgct
     8221 tgcccgtgat ccgcaacagc gcttcgcatc gatcaacgac atgcgtgagg cgcttgatgg
     8281 ctatctggaa ccggaggagc ccgtagcttc agcggataca aaacagagca cgattgattt
     8341 tctgttgcgg cgcatgcgtc ataagagtga tttccctgcg ctatcggaat cggtcggcac
     8401 aatcaataaa attgccggtt ccgacaagga aagcatctcc aatctctcga actcgattct
     8461 caaagatttt tcgctgacca acaagatcct gcgcctggtc aattccgcct attatcgtca
     8521 ggtcggcggt ggaagcatca gtacgatatc gcgcgccgtg ctggtactcg gtttcgacgc
     8581 cgtacgcaac attgcgatca cggtaatgct cttcgaacac ctgcaaaaca agtccaacgc
     8641 caagcagctc aaagaggaat ttctgcgcgc gaacctagcc tctattctgg ccaaggatat
     8701 cagcgaaaaa tcgtcggcgc gagacgttga gcaggtgttt atctgctcga tgtttcacaa
     8761 cctcggacgt ctgttgagcc agttttactt cccggatgaa agtactgaaa tcaaacggat
     8821 gatcgagcag aagttgtgca gcgaggaagc ggcagcggta cagacgctcg gtatctctta
     8881 cgaggatcta ggcatcggca ttgcccgtac ctggggtttc cccggtctga ttgtaaatag
     8941 catgcgcaaa ttgcccgcgg gtaacgttcg caaggcaagt accgtcgagg aaaggatgcg
     9001 tgttctatcg gcattcgcca acgacctgtg tggattgatc gccgatgtcg agcctgagca
     9061 gcgacagaag gaactgcgta aggtcatgtc tcgctttggc gagaacattt cgctgaatga
     9121 acaggcgttg cgcgagacga tggaaaaatc gatgcaggag gtggtgctgt tcgccggaat
     9181 cattggcgtc aatctgaaac aatccgagtt tggccggcag atgaagacgt ggagagcgaa
     9241 gtcgggggcc gccgagggtg cggaagagga aaaaaggatc gccgctgaag gcgcggaaac
     9301 cgtgctttca gacgttgtcc cggcgggtgc gccgggcagt ccggtaattg cgggtgaaga
     9361 aattgaagcg ggtgaagaag gtgtctcagg ggcggcagct cagatcggga attcagaagt
     9421 catcctgatg gcgggaattc aggacatcag caatacgctg gtcgaagact tcaagctcaa
     9481 cgacgtgtta cgcatcattc tggaaaccat gtaccgtgcc aagggtttca agcgggtcat
     9541 cctgtgtgtt cgcgatgcac gcaacaactc aatgcttggc cggttcggat tcgggcccga
     9601 cgcgcccgaa attgccaagc gcttcaactt ctctttgacc ttttcgccgg acatttttca
     9661 tgcggctttg tccaagggcg tcgatatcct gatcagcgat accaaggatg cgaaaatttc
     9721 agcgcgtatt ccggaatggt tcagcaaggg ggtcaatgcc gggacctttg tcatcttccc
     9781 cttgagcatc aaaaacaatc cggtcgccat gatctatgcg gactgtgatc ttgccggcga
     9841 gatcgtgatt actgaaaaag agctgtcgct catgcgaacc ttgcgcaatc aggcagtgct
     9901 ggccatcaag cagtcaagtt agtgatcttg aggctcctgg ttggcactca ccaaagcttc
     9961 caccacggtt ccttgcgctc aagcccgcgt gtgtaataca cgctattcgg gtagtttttg
    10021 cgcatcacgc gctctgcgtc gtcgcgcagg tcgttcattc ccatcaggtc gtaagccttg
    10081 accatgatga acagggcttc ttcggtggcc gctgcttccg ggtagtgttt cagggcaaat
    10141 tgggcacggt tggcggcggc gatataggca ccacgcttca tgtagtaacg ggccacatgg
    10201 acctcaagtg aagccagcgc attgaccaga tacttcatgc gcaaaatggc atccggcgtg
    10261 tacttgctgt cgggaaatcg cgtgacaagt tcacggaagg aatcgaagga ctcgcgtgca
    10321 cctttcggat cacgttcggt catgtcctgc tggctgatct ggccgaggat tcccagatcc
    10381 tcgttgaaat tcaccagtcc gcgcaggtag taaacgtagt cgacattcgg atgatttggg
    10441 tggagcttga tgaaccggtc acaggcggcg acggctagag caggttcgtt atccttccag
    10501 taggcgtagg cgatgtcgat ctgcgcttgc tgggcatagc gaccataggg atacctggat
    10561 tcaagctttt caaaatactt gatcgatttt tcgtatgcac catcgttcat ggcttccttg
    10621 gcttcggtat acagcttgtt ggctgaccag ccggccgtct catcctgctg ctcggggaaa
    10681 agtccgcacc cggcgacaag catggcgagg aaaagcgccg tgagaaccgc taaactacgc
    10741 atgatgaaaa gtccagatag agaaacagaa gtcgattcgc ataaggaaaa aaacgggaaa
    10801 gatgccgagc gcgctgatta tagcgcaaac ccccggctcg ctctgagcgt tcccgccgat
    10861 tgcggagggc agcgactgga tcaggttctt gcccgactgc ggccgcagca ttcacgtagc
    10921 aggatgcaga tctggattcg tcagggccgc gtgaccgtgg gtggcgtgac ggtcagcgaa
    10981 cccaggcaga agctttgggg cggaga
//
LOCUS       DAORPM010000056        10908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1346_length_10908_cov_11.274118, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000056 DAORPM010000000
VERSION     DAORPM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1346_length_10908_cov_11.274118"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLD53_04245"
     CDS             complement(<1..509)
                     /locus_tag="PLD53_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60149.1"
                     /translation="MAEQDIIQNMISQLGQSQGDRTPPELDTQFFQVDERDNAKLLAQ
                     ARAQAALMRFYAHDPKQASGDWSEFFPEDDEASLLARQDGSVPPHIGLLGSFFELYRQ
                     PQALLNDFTARHMDFQFKRVLRFEPRPAQPDHAHLLLELKKGVAPLAVTPEQAFSAGK
                     DDSGVELLY"
     gene            complement(531..971)
                     /locus_tag="PLD53_04250"
     CDS             complement(531..971)
                     /locus_tag="PLD53_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HRC60150.1"
                     /translation="MADNAELYDNFLGTGWSFPPRFAGDGVVMSRDEQDIQESLKILF
                     GTVPGERFLEPKFGLDMHALMFEPMSTTLRSLMLDRVRTAILIYEPRIKLINLQIDSP
                     DPNEGSLRVLLEYEVRSTNSRFNLVFPFYRTDANEVLGSVSGAA"
     gene            complement(1000..1278)
                     /locus_tag="PLD53_04255"
     CDS             complement(1000..1278)
                     /locus_tag="PLD53_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HRC60151.1"
                     /translation="MAFAVCVGDISTHGGTIVGPGSATVLIGGKPAAVAGDMHVCSLP
                     PTGHQPTVSVFPSGSSTVMFGGVPALRTTDACICGAMAAVGQPTIMIG"
     gene            complement(1298..3034)
                     /gene="vgrG"
                     /locus_tag="PLD53_04260"
     CDS             complement(1298..3034)
                     /gene="vgrG"
                     /locus_tag="PLD53_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HRC60152.1"
                     /translation="MSETRALPIAATHREFTVKVEDQAMPRENQLLTVSVSLVVNRIA
                     TARLVYLDGNAASSDFPLSNSDLLKPGKRIEILAGAGSGQDSLFKGIIVRLNVKVRDS
                     SAPQLIIDCRHAAMKMTVGRSNSSYFDQSDSEVIEALLGKSGLIGEITATSLKHKQLV
                     QFHTSDWDFMLARAHANGLLIWPSGDKIIVGKPAVNAPPRCTLQFGATLLEFDAEIDA
                     RAQFSAVRSISWDPAQQSLLEVDGNSPGFTGPGNLSGDDLAAVAAAESLELRHAALVE
                     AEAQAWADGEWLRSRVNKVSGRGKCEGIGALKPGDVVVLAGVGTRFNGKVFVSGVRHE
                     FDLVQGWKTHVQFGGLDKLPPDDMSAPPAGALLARVAGLQIGVVVSNEDPDGEDRVRI
                     RLPIVGKDDEGIWARVASLDAGKERGFFFRPEIGDEVVVGFLDEDPRHAVILGMLHSS
                     ALPAPIQGSDDNHEKGYVSREGLRINFNDEKKVIIIDTPAGNSITLSEDDTSIVLADQ
                     NSNTITMNTDGITIESSKALTLKAGTEVQLESGTSFSAKGGTELKLEGGTQAELSSSA
                     VTKLKGGMVQIN"
     gene            complement(3034..3708)
                     /locus_tag="PLD53_04265"
     CDS             complement(3034..3708)
                     /locus_tag="PLD53_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60153.1"
                     /translation="MSASDRGTLEKLLILAYTTPDYSGEPASQFTSYVNPNEITLSYE
                     MEYDAAQGSGTTGSRMNFKKMKPGDLALTFFLDGTGANGERIDVQQKIEEFQTVTGYN
                     GDIHRPNYLKVSWGTLTVKRCVLKSASIAYKLFQPNGIPLRAVITASFTDNSDDTTRV
                     AMAQDQSPDLTHLRVIKAGDKLPVLCNEIYGDPRLYLRVAEANGIDDFRNILPGMRIF
                     FPPLEK"
     gene            complement(3712..3873)
                     /locus_tag="PLD53_04270"
     CDS             complement(3712..3873)
                     /locus_tag="PLD53_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HRC60154.1"
                     /translation="MTIIVDEVVISVEVTNQAAGGGNAPPTATESKQEIVAECVERVL
                     DILREREER"
     gene            complement(3883..4329)
                     /locus_tag="PLD53_04275"
     CDS             complement(3883..4329)
                     /locus_tag="PLD53_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRC60155.1"
                     /translation="MANYYPPVGFHFKVEVLGLPPNDHDVRFTEVSGLSVEMGTEEVA
                     EGGENRFIQKYPTRAKYPELVLKRGLLRNSEVWNWSRQCIEEYRIEPKNVDVKLLNES
                     HEPLMTWHLVGAYPTKWAITDLSASSNTVVIESLQLFYQRFSVDRS"
     gene            complement(4360..4794)
                     /locus_tag="PLD53_04280"
     CDS             complement(4360..4794)
                     /locus_tag="PLD53_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRC60156.1"
                     /translation="MPAQYPLPAFHFTVQWGGTRIGFSEVTGLTQENQAIEYRDGSFP
                     EYSSIKMPGLRKFSNITLKRGIIKSDNDFFNWLSTVKLNTVERRDIVISLLNEKHEPV
                     MVWKAQNAFPVKVEGPALKASGNEVAIESIELAHEGLELQND"
     gene            complement(4842..6794)
                     /locus_tag="PLD53_04285"
     CDS             complement(4842..6794)
                     /locus_tag="PLD53_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HRC60157.1"
                     /translation="MPTYRTPDVYVEEISVFPPSVAEVETAIPAFVGYTEFARRIVGG
                     DLVNVPTRLKSLLEYEALFGKGPQIVVTKVVLDDANNFVRAEVSSNFYLYDSLRLFFD
                     NGGGDCYVVSVGADNYQTAPDHTKLKAGVEALKKYDEPTILLFPDAASLSADNMAVVQ
                     QAALAQCGALGDRVTVLDTRSDDPLGTAFRDKLGINHLKYGAAYTPWLKLNYSKNVGY
                     ANIKSVVEKGGSAVTLQSLTPDADLLALMKSIDDAQADVDGFAARIATATGASGKSVT
                     ARQAELMTEYRSSPTAADMQNLFAYLYKLAELVDACANGGVPLAHVKLRNDAGASISS
                     PLKDAFGKLIAFEQELKSRMDAGPFAYTLQWSAGLNADGPQWDGIFAATPPAASTTGI
                     PPALTAETELLNASLPSINAAFSAASGVINSLVTGASSYRSTYEQSLLENFGVYSNLI
                     KGINNTLTSCPPSGAIAGIYSLIDNQRGVWKAPANVSLNSVIGPLVNFDTSDTDGLNV
                     DAVAGKSINAIRAFSGKGTLVWGARTLAGNDNEWRYISVRRFFNMVEESVKKSTYWAV
                     FEPNDANTWVKVRGMIENYLTQKWREGALAGATPKEAFFVRCGLGVTMNAQDILEGRM
                     NVQIGMAVVRPAEFIILQFSHKLQTS"
     gene            complement(6805..7806)
                     /locus_tag="PLD53_04290"
     CDS             complement(6805..7806)
                     /locus_tag="PLD53_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60158.1"
                     /translation="MNISFAPLAKVSPVHGYYSQACSDIEFMPSRSTHELLRGGRMLA
                     RMLNGELHLLYEAESPGVPLSSLAGQTLHFGLRLANPVFSNITMPVVTDTTLTPLYAN
                     ATLPATLDAPQGVELTAGLYGLAPKLASRPVTLRLLDAHANILETRQLAEAATSYDTR
                     GLAPGEYRIEEDYGAGLLHARKLFVDASLRDTGVWGLLALRIDAAFYTNAANFTLNFA
                     VRKETLRYYVVARNWQPDEFNQLNIVDEGFSAEGRDEITFTRLVAPFPDGFIKDSLLG
                     DSSVQIAVFQSQTEVARKERGLRKLHLSRNSTVLIEHLPLPGPERAKADFIVHLSKP"
     gene            complement(7803..8387)
                     /locus_tag="PLD53_04295"
     CDS             complement(7803..8387)
                     /locus_tag="PLD53_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HRC60159.1"
                     /translation="MLDIAIKFLADEVNTHLKKRALSASGLGEVLTGQIVDDAGKWAT
                     AADNIGLTLVNIEEDRIMREHLPEHAYVNGSHVVLQPEIKLNLSVLFHTRPSKADKYE
                     QSLRFLSNLITFFQAHPVFTPEEYPGLDPRINKLIVEMLALSTEQLNQMWAYLGSKYL
                     PSVAYRVRMVLLQDVEPQSIGKPITIIETALHDK"
     gene            complement(8741..10204)
                     /gene="amt"
                     /locus_tag="PLD53_04300"
     CDS             complement(8741..10204)
                     /gene="amt"
                     /locus_tag="PLD53_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRC60160.1"
                     /translation="MKNLLVILALFGAVTCTAPAWAEEKAADAPPATAMATPTSAAAA
                     EAPAAPAFAMNKADNAWIMVCAALVILMSIPGLALFYGGLVRSKNMLSVLMQVFVTFS
                     MISVLWVVYGYSMAFTEGGSFFGTLDKLFLKGVTPESLGATFSKGVYISELTFVIFQG
                     AFAAITCGLIVGAFAERAKFSAILVFMLIWFTFSYLPMAHMVWYWAGPDAYIDAAAAE
                     AAGKTAGFLFQKGALDFAGGTVVHINAAVAGLVGAFMIGKRVGYGKESMAPHSLTFTM
                     IGASLLWFGWFGFNAGSALEASGGSALAMVNTWVATACAAMSWMLAEWLIKGKPSMLG
                     AASGAVAGLVAITPAAGFVGVMGAIIIGLLAGIVCLWGVNGLKRMLGADDSLDVFGVH
                     GVGGILGAILTGVFCAPSLGGTGVYDYVTNAVGDYDMGAQVINQLWGVGTTVVWSGLV
                     SLVAFKVVDMFIGLRVPEEEEREGLDITSHGESAYHI"
     gene            complement(10215..10553)
                     /gene="glnK"
                     /locus_tag="PLD53_04305"
     CDS             complement(10215..10553)
                     /gene="glnK"
                     /locus_tag="PLD53_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRC60161.1"
                     /translation="MKMVTAIIKPFKLDEVREALSVIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVDAAIQDSMLDQVIEAIEKSAGTGKIGDGKIFVFDLEQVIRI
                     RTGETGEEAL"
     gene            complement(10605..>10908)
                     /locus_tag="PLD53_04310"
     CDS             complement(10605..>10908)
                     /locus_tag="PLD53_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60162.1"
                     /translation="NYDLGDGWGVNGHVGTLNLKNVHNGDYTDWKIGVTKDISGWVVA
                     LSYVDTNASGKCSGASTYQPYCFYSSLSDSNGTLQTSSHTKDAGNGIAIVSVSKSF"
BASE COUNT         2124 a         3164 c         3289 g         2331 t
ORIGIN      
        1 ttgtagagca gttcgacgcc gctgtcgtcc ttgcctgccg aaaaggcctg ctcgggtgtg
       61 accgctaacg gcgccacgcc cttcttgagc tcgagcagca gatgcgcgtg gtcgggctgt
      121 gccgggcgcg gctcaaaacg cagcacgcgt ttgaactgaa aatccatgtg ccgcgccgtg
      181 aaatcattga gtagcgcctg gggttgtcga tatagttcga agaagctgcc gagcaggccg
      241 atgtgcggcg gaacgctgcc gtcctgacgg gcgagcagac ttgcttcatc gtcttccggg
      301 aagaactcgc tccagtcacc actcgcctgc ttcgggtcat gcgcatagaa gcgcatcagc
      361 gcggcctgtg cgcgggcttg cgccagcagt ttggcgttat cacgctcgtc gacctggaag
      421 aactgcgtgt cgagctccgg cggcgtgcga tctccctggc tctggccaag ttgcgagatc
      481 atgttctgga tgatgtcctg ttcagccacg gcaagtgatt cctgtcattc tcacgcggcg
      541 cccgacaccg aaccaagaac ttcattggcg tcggtccgat agaacgggaa gaccagattg
      601 aagcgcgagt tggtgctgcg tacttcgtat tcgagcagca cgcgcagcga gccttcgttg
      661 ggatcggggc tgtcgatctg cagattgatc agcttgatgc gcggctcgta gatcaggatc
      721 gccgtacgca cgcggtcaag catcaggctg cgcaaggtgg tgctcatcgg ttcgaacatc
      781 agggcatgca tgtccagccc gaatttcggt tccagaaaac gttcgccggg cacggtgccg
      841 aagaggattt tcagactttc ctgaatatcc tgttcgtcgc ggctcatgac cacgccatcg
      901 ccggcaaagc gcggcgggaa gctccagccc gtgccaagga agttgtcata gagttctgcg
      961 ttgtcagcca tcagattccc tttgcgctca ttgatgtctt cagccgatca tgatggtggg
     1021 ttggccgacc gccgccatcg cgccgcaaat acacgcgtcc gtcgtgcgca gggcgggaac
     1081 gccgccaaac ataaccgtac tgctgccgct ggggaaaacg ctgacggtgg gctggtgtcc
     1141 tgtgggcggc aaagagcaca cgtgcatgtc gcctgccacg gctgctggct tgccgccaat
     1201 cagtacggtg gccgaaccgg ggccgacgat ggtgccgcca tgggtgctga tgtcgcctac
     1261 gcagaccgcg aatgccattt atatgcctcc tgtcgcctca attgatctgc accatgccgc
     1321 ccttgagctt ggtcaccgca ctgctcgaca gttcggcctg ggtgccgccc tcgagtttga
     1381 gttcggtgcc gcccttggcg ctgaacgagg tgcccgattc cagttgcacc tcggttcccg
     1441 ctttcagcgt gagcgccttg ctgctctcaa tggtaatgcc atcggtattc atggtaatgg
     1501 tgttgctgtt ctggtcagcg aggacgatgg acgtgtcgtc ttcgctgagc gtgatgctgt
     1561 tgcctgccgg cgtgtcaatg atgatgacct tcttctcgtc gttgaagttg atgcgcagcc
     1621 cttcgcggct gacatagccc ttctcgtggt tgtcgtcgga cccctggatc ggtgcgggca
     1681 gcgcgctgct gtgcaacatg ccgagaatca ccgcgtgacg cggatcttca tcgaggaagc
     1741 cgaccacgac ttcgtcgccg atctcgggac ggaaaaagaa ccctcgctcc ttgccggcgt
     1801 ccaggctggc cacgcgagcc cagatacctt cgtcatcctt gccgacaatc ggcaggcgga
     1861 tccgtacgcg gtcctcgccg tccggatctt cgttgctcac cacgacaccg atctgaaggc
     1921 cggcaacgcg ggcgagcagg gcgcctgcag ggggggccga catgtcgtcg ggcggcagct
     1981 tgtcgagtcc gccgaattgc acgtgcgttt tccagccttg caccagatcg aattcatggc
     2041 gcacgccgct gacgaaaacc ttgccgttga agcgggtgcc gactccggcg aggacgacga
     2101 cgtcacccgg cttgagcgca ccgatgcctt cgcacttgcc gcgaccgctg accttgttga
     2161 cgcgggagcg cagccattca ccatcggccc aagcctgcgc ctcggcttcg acgagggcgg
     2221 catggcgcag ttccagcgat tcggctgcgg ccacggccgc aagatcgtca ccggaaagat
     2281 tgcccggccc ggtgaatccg ggcgaattgc cgtcgacttc gagcaggctt tgctgggccg
     2341 ggtcccaact gatgctgcgc acggcagaga actgtgcgcg cgcatcgatt tcggcatcga
     2401 actccagcag cgtcgcaccg aactgcaagg tgcaacgcgg cggcgcattg acggccggct
     2461 tgccgacgat gatcttgtcg ccggaaggcc atatcaacag gccgttggca tgagcgcggg
     2521 cgagcatgaa gtcccagtcg ctggtgtgaa actgcaccag ttgcttgtgt ttgagcgatg
     2581 ttgccgttat ctcgccgatc aggccggact tgccaagaag cgcttcgatc acctcgctgt
     2641 cggactggtc gaagtagctc gaattgctgc gccccacagt catcttcatc gccgcatggc
     2701 gacagtcgat gatcaattgc ggcgcactgg agtcgcgaac cttcacgttc agccgcacga
     2761 tgattccctt gaacagcgag tcctgaccac tgccggcacc ggcgaggatt tcgatgcgct
     2821 tgccgggctt gagcagatcg ctgttgctga gcgggaaatc gctgctggca gcatttccgt
     2881 ccagatacac cagacgcgcc gtggcgatac ggttgaccac cagactgacg cttactgtca
     2941 gcaactggtt ttcgcgcggc atcgcctgat cctccacctt gacggtgaat tcgcgatgcg
     3001 tagcggcaat cggcagggcg cgtgtttccg acatcatttt tccagcggcg gaaagaagat
     3061 tctcatgccg ggaaggatgt tgcggaaatc atctatcccg ttcgcctcgg ccacgcgcag
     3121 gtagaggcgc gggtcgccat agatctcgtt gcacagaacc ggcaatttat cgcctgcctt
     3181 gatgactctc aggtgggtca ggtcgggaga ctgatcctgt gccatcgcca cccgcgtggt
     3241 gtcatcggaa ttgtcggtaa agcttgccgt gatgaccgca cgcagcggta tgccattagg
     3301 ctggaacagt ttgtaggcaa tcgatgcgct cttgagcacg cagcgcttta ccgtgagcgt
     3361 gccccagctc accttgaggt agttgggacg gtgaatatcg ccgttgtagc cggtgaccgt
     3421 ctggaactcc tcgatcttct gctgcacatc gatgcgctcg ccattggccc cggtgccgtc
     3481 aagaaagaaa gtcagggcga ggtctcccgg tttcatcttc ttgaagttca tgcggctacc
     3541 cgtcgtgcct gaaccctgtg ccgcgtcata ctccatctcg tatgacagag tgatttcgtt
     3601 cgggttgacg taggatgtga actgactcgc cggctcgccc gagtaatcgg gtgtcgtgta
     3661 ggcgaggatc agcagcttct ccagcgtgcc gcggtcagag gcgctcatga tctatcgttc
     3721 ctcacgttcg cgcaggatgt cgagcacacg ttcgacgcac tcggcaacga tttcctgttt
     3781 gctctcggtc gccgtaggcg gcgcgttgcc gccgccggca gcctggttgg tgacttccac
     3841 cgaaataacg acttcatcga cgatgatggt catggtgcgt cctcaggagc gatccacaga
     3901 aaagcgctgg taaaacagtt gcagcgattc gatgacgacg gtattgctcg aagcgctcag
     3961 atcggtaatc gcccattttg tcgggtaggc accgaccaga tgccacgtca tcagcggttc
     4021 gtgcgactca ttgagcagtt tgacatccac attcttgggc tcgatccggt attcctcgat
     4081 gcattgccgt gaccagttcc acacctccga attccgcaag agcccccgct tcaagaccag
     4141 ctcgggatac tttgcgcggg tcggatactt ctggatgaag cggttctcgc caccttccgc
     4201 gacctcttcg gttcccattt ccaccgaaag tccgctgacc tcggtgaagc gcacgtcgtg
     4261 atcgttgggc ggcagtccca gaacctccac cttgaagtgg aatccgaccg gtgggtaata
     4321 attggccatg gcgaaatgcg tttggctggc taggatctat cagtcgttct gcagttccag
     4381 tccttcgtga gccagttcga tggattcgat ggccacctcg ttgcccgagg ccttgagcgc
     4441 aggcccctcg accttgaccg ggaaggcgtt ctgcgctttc cagaccatca ccggctcgtg
     4501 cttctcgttg agcaggctga taacgatgtc gcggcgctct acggtattga gcttgacggt
     4561 ggagagccag ttgaaaaaat cgttgtcgct cttgatgatg ccgcgcttga gcgtgatgtt
     4621 gctgaacttg cgcaggccgg gcatcttgat tgacgagtac tccggaaacg atccgtcgcg
     4681 atattcgatg gcctggttct cctgcgtcag gccggtgact tccgagaagc cgatgcgtgt
     4741 gccaccccac tgcacggtga aatgaaacgc gggtaatgga tattgagctg gcatgatgag
     4801 ttgtccttag gtctgaggtt gatcggtcga tgccaatggg gtcaggaggt ctgcaacttg
     4861 tgcgagaact ggaggatgat gaattccgcc ggacgcacga cagccatgcc gatctgaacg
     4921 ttcatgcggc cttcgaggat gtcttgggcg ttcatcgtca cgcccaggcc acagcgcaca
     4981 aagaaggctt cctttggcgt ggcgccggcg agtgcgcctt cgcgccattt ttgtgtcagg
     5041 tagttctcga tcatgccgcg taccttgacc caggtattgg cgtcattggg ctcgaatacc
     5101 gcccagtagg tcgacttctt gaccgattcc tcgaccatgt tgaaaaaacg gcgcaccgag
     5161 atatagcgcc attcattgtc atttcctgca agcgtgcgcg ccccccacac cagggtgccc
     5221 ttgccggaaa atgcgcgaat ggcattgatc gacttgccgg cgaccgcgtc gacgttgagc
     5281 ccgtcggtgt ccgaagtgtc gaaattcacg agcgggccga tgacgctgtt cagactcaca
     5341 ttcgccggcg ctttccagac gccacgctgg ttgtcgatca gtgaatagat accggcgatc
     5401 gccccgctgg gcggacaact ggtcaaggta ttgttgatgc ccttgatgag attgctgtaa
     5461 acaccgaagt tttcaagcag gctctgctca taggtgctgc ggtaactcga cgcgccagtg
     5521 accagactgt tgatgacgcc gctggcggct gagaatgccg cgttgatcga cggcagggaa
     5581 gcgttgagca gttcggtttc tgcggtcaag gcgggcggta tgccggtagt gctcgctgcc
     5641 gggggcgtgg ctgcgaatat gccgtcccac tgcgggccgt cggcattgag ccccgcgctc
     5701 cactgcagcg tgtaggcaaa aggtccggca tccatgcggc ttttgagttc ctgctcgaaa
     5761 gcaatcagtt tgccgaaggc atctttcagc ggtgaggaaa tacttgcgcc ggcgtcgttg
     5821 cgcagcttga cgtgagctag cgggacaccg ccgttggcgc aggcgtcgac cagttccgct
     5881 agtttgtaca gataggcgaa caggttttgc atgtctgccg cagtcgggct ggaacggtac
     5941 tcggtcatca gctcggcctg gcgtgcggtg acactttttc cgctggcgcc ggttgccgtt
     6001 gcgatgcgcg cagcgaaacc gtcaacgtca gcctgtgcgt cgtcgatgga cttcatcagc
     6061 gccagtaaat cggcatccgg tgtcaggctt tgcagcgtga ccgccgatcc gcctttttcg
     6121 acgactgact tgatgttggc atagcccaca ttcttgctgt agttgagttt gagccaaggg
     6181 gtgtaggcgg cgccgtactt gaggtgattg attccaagtt tgtcgcggaa tgcggtgcct
     6241 agcgggtcgt cactgcgggt gtcgagcacc gtcacgcggt cgcccaacgc accgcattgc
     6301 gccagcgcgg cctgctgaac gacggccatg ttgtcggccg acagcgaagc cgcgtcgggg
     6361 aacagcagga tggtcggctc gtcatatttc ttgagcgcct cgacaccggc cttcagtttg
     6421 gtgtggtccg gggcggtttg gtaattgtcg gcaccgacgg atacaacgta gcaatcgccg
     6481 ccgccgttgt cgaaaaacaa acgaaggctg tcgtacaggt agaaatttga actcacttcg
     6541 gcgcgtacaa agttgttggc atcgtcgagg accaccttgg tcacgacaat ttgcggtccc
     6601 ttgccaaaca gggcctcgta ctcgagcagc gatttgaggc gcgtaggcac attcaccagg
     6661 tcgccgccga caatgcgtcg ggcaaattcg gtgtagccga caaaagcggg aatggccgtc
     6721 tcaacctcgg caaccgaggg cggaaagacg gagatttctt cgacgtagac atccggagtt
     6781 cgataagttg gcatggttgg gcctctaggg tttggacagg tgaacgatga aatctgcctt
     6841 cgcccgttca gggcccggca gcggcaagtg ctcgatcaga acggtagagt tgcggctgag
     6901 gtgcaatttc ctcagaccgc gctctttccg cgcgacttcg gtttgggact ggaataccgc
     6961 aatttgtacg ctgctgtccc cgagcagcga atccttgatg aagccgtccg ggaaaggcgc
     7021 gacaagacgg gtgaatgtga tctcgtcccg cccctcggcg gaaaatccct cgtcaacgat
     7081 gttcagttga ttgaactcgt cgggctgcca gttgcgggca accacgtaat agcgcaaggt
     7141 ttccttgcgc acggcaaaat tcaatgtgaa gttcgccgca ttcgtataga aggcggcatc
     7201 gatgcgcaat gccagcagac cccatacgcc tgtatcgcga agactcgcgt cgacgaacag
     7261 cttgcgcgca tgcagtagcc ccgcgccgta atcttcctcg atccggtact cgcccggtgc
     7321 caggccgcgc gtgtcgtaac tcgttgcggc ttcggcaagc tgtcgtgttt ccaggatgtt
     7381 ggcatgtgcg tcgagcaggc gcagggtgac cgggcgcgac gcaagttttg gtgccaggcc
     7441 gtaaaggccg gcagtaagct ccaccccctg cggtgcatcg agcgttgctg gcagtgtggc
     7501 atttgcatac agcggcgtca gtgtggtatc ggtgaccacc ggcatggtga tgttgctgaa
     7561 gaccgggttg gccaggcgca ggccaaaatg cagtgtctgc ccggccaggc tactgagcgg
     7621 cacgccggga ctctcggcct catacagcaa gtgcagttcg ccattgagca tgcgtgccag
     7681 catgcggccg ccgcgcaaca attcgtgggt actccgactg ggcatgaatt cgatatcact
     7741 gcatgcctgg ctgtagtagc catgcaccgg tgaaactttc gcgagcgggg cgaagctgat
     7801 attcatttgt cgtgcaacgc cgtttcgatg atcgtaatcg gcttgccgat gctttgcggt
     7861 tcgacatcct gcagcagcac catgcgcacc cggtaggcta ccgagggcag gtacttgctg
     7921 cccagatagg cccacatctg gttgagctgc tcggtagaga gcgcaagcat ctcgacgatc
     7981 agcttgttga tacgtgggtc gagcccgggg tattcctccg gtgtgaacac cggatgtgcc
     8041 tggaagaaag ttatcaggtt cgacagaaac cgcagggact gctcgtactt gtcggccttc
     8101 gaagggcgtg tgtgaaagag cacgctgagg ttcagcttga tttcgggttg cagtacgaca
     8161 tggctgccat tcacatacgc atgctcaggc agatgctcac gcatgatgcg gtcttcttcg
     8221 atattgacca gtgtcaggcc gatgttgtcg gccgcagtcg cccacttgcc cgcgtcatcg
     8281 acaatctgtc cggtgaggac ctcaccgagt ccgctcgccg acaaggcgcg ctttttcagg
     8341 tgggtattga cttcgtcagc aagaaacttg atcgcaatgt ccagcatcgc tgctccagtt
     8401 cgtcctgatc cgaatggcgt tctcgttcat tgtcgagtca cttgcagccc ttggctgtgt
     8461 acctggttgg tgtgcaaagt gaagcgccgc cgcgtgttga actcattacc tgacagcatg
     8521 gttggatcgg agttcccgga ggccatactc gaatacattt tcgggcgatg ctacccgaag
     8581 cgatttgatc acgcaatgct gagcggtttg aaaaactgct cgtcgtgccg accatcgatt
     8641 caccagttct tcttgatggt ccgagttgga tttggttgca gaagccacat aaaaaagggc
     8701 gccacaaggg cgcccaaagg aggttgtctg caaaaagata tcagatgtgg taagccgact
     8761 cgccatgcga agtgatgtcc agaccttcgc gctcttcttc ttccggcaca cgcagaccga
     8821 tgaacatgtc gaccaccttg aaggcgacca acgaaaccag gccggaccac acgactgtcg
     8881 tacccacgcc ccacaactgg ttgattacct gggcgcccat gtcgtaatcg ccaaccgcat
     8941 tggtcacgta gtcatagaca ccggtaccgc cgagcgaagg ggcacagaaa acacccgtta
     9001 ggatggcgcc gagtatgccg ccgacaccat gcacgccgaa cacgtcgagc gagtcgtcgg
     9061 ccccaagcat cctcttcagg ccattgacgc cccacaggca gacgatgcca gccagcaggc
     9121 caatgatgat ggcgcccatg acaccgacaa aaccggccgc cggggtgatc gccaccagac
     9181 cggctaccgc gccggaagct gcacccagca tcgaaggctt gcccttgatc agccattcag
     9241 ccagcatcca ggacatggcc gcacaagccg tggcaaccca ggtattgacc atcgccaggg
     9301 ccgagccgcc cgatgcttcc agagcggatc cggcgttgaa accgaaccag ccaaaccaca
     9361 gaagtgaggc gccgatcatg gtgaaggtca gactgtgcgg ggccatcgat tccttgccgt
     9421 aaccgacccg tttgccgatc atgaaggcgc ctaccagccc ggcgacagcc gcattgatat
     9481 gcaccacggt accgccggca aagtcgagcg cgcctttctg gaacaggaag cctgccgtct
     9541 tgcccgcggc ctcggcagcc gctgcgtcga tataggcgtc cggacctgcc caataccaca
     9601 ccatatgcgc catcggcagg taggagaagg tgaaccagat gagcatgaag accaggatgg
     9661 cggagaattt ggcacgctcg gcgaacgcac cgacgatcag tccgcaggtg atggccgcga
     9721 aggcgccctg gaaaatcacg aaggtgagtt ccgagatata gacccccttg ctgaaggtgg
     9781 ctcccaacga ttcgggcgtg acgcccttca ggaacagctt gtcaagcgtc ccaaagaagc
     9841 tgccaccctc ggtaaaggcc atcgagtagc catagactac ccagaggacg ctgatcatcg
     9901 agaaggtgac aaagacctgc atcagcaccg atagcatgtt cttggaacgg accagtccgc
     9961 cgtagaacaa tgccaggccc gggatcgaca tcaggatcac cagcgccgca cagaccatga
    10021 tccaggcgtt gtccgccttg ttcatcgcga atgctggtgc ggcaggagct tctgctgcgg
    10081 ccgctgaagt tggcgtggcc atggccgtgg ctggcggcgc gtctgccgcc ttttcttctg
    10141 cccatgccgg tgcggtgcag gttacggcac caaacagagc gagtatgact aagaggtttt
    10201 tcatgatgcg ctcctcacag ggcttcctca ccggtttcgc cggtgcggat gcggatgacc
    10261 tgttcaaggt cgaaaacaaa gattttgccg tcgccgatct ttccggtgcc ggcggatttt
    10321 tcgatggctt cgattacctg gtccagcatc gaatcctgaa tcgcggcatc gactttgacc
    10381 ttcggcagaa aatcgacgac atactcggcg ccacgataaa gctcggtgtg acctttctga
    10441 cgaccgaagc ccttgacctc ggtgaccgtg atgccctgta cgccgatgac ggaaagcgct
    10501 tcacgcactt cgtcaagctt gaagggcttg atgattgcgg taaccatttt catgatgtgc
    10561 tccaattctg gggagggtga cacctccccg gttacaaaaa gatgtcagaa tgacttgctg
    10621 accgaaacga tggcgatgcc gttacccgcg tccttggtgt gcgagctggt ctgaagcgta
    10681 ccgttgctgt cggaaagcga actatagaaa caatagggtt gataggttga cgcgccagag
    10741 catttgcccg aggcattggt gtcgacatag gaaagcgcca ccacccatcc ggaaatgtcc
    10801 ttggttacgc cgattttcca gtccgtgtag tcgccgttgt gaacgttctt caagttgagc
    10861 gttccgacgt ggccgttgac gccccagccg tcacccagat cgtagttg
//
LOCUS       DAORPM010000057        10872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1361_length_10872_cov_9.894888, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000057 DAORPM010000000
VERSION     DAORPM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10872
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1361_length_10872_cov_9.894888"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLD53_04315"
     CDS             <1..488
                     /locus_tag="PLD53_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60163.1"
                     /translation="KVLPAGNKAWRNTSIKARPISSIVLSRASTVVNLLGEGDLTLAG
                     KCRRHGQVPLEFRIPNSSIVMPEVTLCNLHKCWPQAGIEACIPLYSACESASIRGQAR
                     TQLRRLLPRLAYNSSNCHPTNGISCLQGLFVVIRRFTCDGKALFAGTRYENDICPTFS
                     F"
     gene            460..1890
                     /locus_tag="PLD53_04320"
     CDS             460..1890
                     /locus_tag="PLD53_04320"
                     /inference="COORDINATES: protein motif:HMM:NF022518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HRC60164.1"
                     /translation="MTSAQPSHFEKLWLAESLRQLETDAWLDDKAILMRLATENADEE
                     YGTEALILERTRLLAEKHKTAAAMATEIRGVRSGASVVATVFGVAAVLGGIATASVVL
                     GNPSVNVILAWFALLGPHFVSLIVWGGGLLFGMHSARFSLGSLWSAATRRFATGGHTP
                     LLFQAVASLCVRTRLPYWAYSLISHALWSLFFIGAILGLSYLLMIREYNFRWETTILP
                     SEFFAGFVAFFGWLPKLIGFQIPEPDVVIHLPGGETSRRAWSAWLLCGVVFYGFLPRV
                     VLALDGLRRLKRIRRGLTLDLELPYYVRLRQRLDALLRPAVTITDPDSGVPDLAIQRR
                     SVSSGDIIDRGSGFSPPLLFALELHDDVVWPPSHLPDGIVVAGNISTLDSRDALLNRI
                     AQIKPRRVVVACEARATPDRGTLFLLNDVSNVAEAVGVLLLNQSSARADRARYWREEL
                     AAVGIKAELIFPELSESLVWLKGCNE"
     gene            1883..3271
                     /locus_tag="PLD53_04325"
     CDS             1883..3271
                     /locus_tag="PLD53_04325"
                     /inference="COORDINATES: protein motif:HMM:NF023407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HRC60165.1"
                     /translation="MNKSSSRPISVAVVGHTNAGKTSLLRTLSRDKNFGNVSGQPGQT
                     RHVEATSVSIDGRPVLKLFDTPGFEDSIALQEYLKQFENRESRRSSLAAFLGSPEAKG
                     RFVQEAKILGALLGDIDAAFYVVDTTETPHAKFRSELDILASCAVPIMPVLNFTGHPD
                     SRLGEWKSVLADRGLHVQVAFDVIAPLAGSERRLYEQLASLLGEHRAKLESLVVALAN
                     ESKARSHASFRSIGDLLVDVAAYRTQVVEKNDDEIAREVSELQSKVRQREQRCADALL
                     SIYRFDRNDLEVRDLPLLSERIEDDLFNPEVLKQTARRLGRGAAIGALVGLAADIGVA
                     GISLGSGAVIGGAVGGMVVSSWKKSARWVKEKINGYTDLTVDDATLLVLLGRQLQLLT
                     SLQGRTHAANERLVLDGNPALAKALEPVIEKTIIARGHPDWSRLSDGFMDESDRVEVV
                     ESIADGIGEIVV"
     gene            complement(3296..3826)
                     /locus_tag="PLD53_04330"
     CDS             complement(3296..3826)
                     /locus_tag="PLD53_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRC60166.1"
                     /translation="MKRTTGIWFGLFFVLVGAGWIIWSNERILADGRVVLLELAPIDP
                     RSLMQGDFMSLNYALGNELRRKSAREDGYAVVRLDSRGVATLLRVQSEPLKPLQALPN
                     QSDTELPVIDEIAIRYRIRNHRPMVATNAFFFQEGDASRFANARFGVFRVASNGEPRL
                     TALLDDNLKRLGENRY"
     gene            complement(3823..4860)
                     /locus_tag="PLD53_04335"
     CDS             complement(3823..4860)
                     /locus_tag="PLD53_04335"
                     /inference="COORDINATES: protein motif:HMM:NF025709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HRC60167.1"
                     /translation="MLEPVLNELRADGYLRGESLPPPSFEAGGSHWILALQVIGGWLA
                     AVFMLLFLGMGAVPLVKGASNWIVIGLIVTALTGLLMGRVVDTGSTVLRQFLLVASMA
                     GHGALIVGATMFGRGEGSTSFFMIALYEAVMLLWVAWMPHRLVAALVGSAALVMAVDM
                     SISWELVRYWVGIYWFAVCLLWYHETRWQAHRFGESVYALACALTLLCFAYALSGYFS
                     HNLFGLHKAFHFDAVLCSAVNIAFVLIIARPQLKSVRNMFAAVLLAIALGMTWQAPAV
                     GMGALTLVFGFARGHRWLMWLGGAMLVFGVGRFYYDLQLILLHKASLMFLGGILLLTV
                     RALIRKQEKPA"
     gene            complement(4853..5866)
                     /locus_tag="PLD53_04340"
     CDS             complement(4853..5866)
                     /locus_tag="PLD53_04340"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HRC60168.1"
                     /translation="MTPLTARRYLESLARNGQLTAKGLDFALHRIGVFPDAANWKRFA
                     DRLLLWAGILLLLASVVFFFAFNWNELHRFTKISLVVLPLVISSSMMAYTGIERAAGK
                     AWLGAGVVLTGVLLAVIGQIYQTGADSELLFAGWALLALPWVVIARAPWVWLFWLVLL
                     NTSLALFLVGRFDIWMVLIYSDAIFWGPLLLNALALLLWEFYWPRIAWMRARYAPRFI
                     VFLAAVAATGLGVSWWFLVKRTGWQLMHYAPIAYLPWLFLTVWYYRTRRKDIVPLVAS
                     ALSCIAVLTAGLISGMFGKKYLDVTSFFVIGAVVAAMTAAAAIWLRHTSSKWAKARGV
                     GDA"
     gene            complement(5966..6631)
                     /locus_tag="PLD53_04345"
     CDS             complement(5966..6631)
                     /locus_tag="PLD53_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRC60169.1"
                     /translation="MNVSIDIVSDLVCPWCFIGKHRMTGAVALVREKFPETRFRLNWL
                     PFFLNPDTPPEGEPYRAFLEAKFGGKEQVSSLQKVVTEAGRDTGVEFDFERISIRPNT
                     LHAHRLIYRAQSTGRQPAEIEALVERLFVAHFQRGEDIGDIATLAEIAAECGDRMDDV
                     TEYLESTMDVDQVRSLFDGISGQGISGVPLFVIQDRLAVPGAQSSAVLAAAILQAMEA
                     PSS"
     gene            complement(6681..7142)
                     /locus_tag="PLD53_04350"
     CDS             complement(6681..7142)
                     /locus_tag="PLD53_04350"
                     /inference="COORDINATES: protein motif:HMM:NF008742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HRC60170.1"
                     /translation="MSTIGNILWLILGGLLMGLGWWLAGLLAFVSIIGIPWGKACFVI
                     GQFAFLPFGKEAVSREELSGQSDIGTGVLGLFGNIVWFLLAGVWLAIGHVISAAACFV
                     TIIGIPFGIQHLKLAAIALAPIGKTIVNKEVAAAMRGESAQSELARLRQSN"
     gene            complement(7144..7545)
                     /locus_tag="PLD53_04355"
     CDS             complement(7144..7545)
                     /locus_tag="PLD53_04355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRC60171.1"
                     /translation="MLVIKSLSPIRWGDMDAYHHVNNTIYFRYMEQARVEYLESLGYS
                     IVPKGTAPVIINASCTFLIPLTYPGTVEVSMFFGPPGRSSFMSSYEIRMQGEDALYAT
                     GDAKIVWTDVASGKSVPIPEELRTRLSEIGS"
     assembly_gap    7546..7645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7664..7879)
                     /locus_tag="PLD53_04360"
     CDS             complement(7664..7879)
                     /locus_tag="PLD53_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60172.1"
                     /translation="MRTVSAIVISTLVLLSGCGIESASTVATVGKLKADEAKQGKEQM
                     ETFKANLNAATKASEESVKRSEEAYKN"
     gene            complement(7916..8773)
                     /locus_tag="PLD53_04365"
     CDS             complement(7916..8773)
                     /locus_tag="PLD53_04365"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRC60173.1"
                     /translation="MSQTIHEAAESLRQAVGTANIGELSFPTSLDASRRVLKAVENPD
                     LGMAALAKIVVAEPLLSAKVIRLANSVALNPTNQTVRDVRHAVMRVGMDPIKGLAMVL
                     IMDQLRQSQRHSGCRDLSNRLWERSVHVAALSYVLARKLTKLNADEVMFAGIVHDLGR
                     FYLLSRVADFPALLEDTVVLAETINDLAGHATEIVLNELKLPASIVDAVLASKQFSGS
                     MPPASLSDLLFIAGAVSPRPDPFDELDARVTPMADSAATLGLDQSTVAEVIAASGDEI
                     YSIVIALES"
     gene            complement(8966..10759)
                     /locus_tag="PLD53_04370"
     CDS             complement(8966..10759)
                     /locus_tag="PLD53_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRC60174.1"
                     /translation="MSEYRAPLRDMQFVLKELAGLEDVAQLPGCEEASTDVVDAVLEE
                     AAKFAEGVLSPLNGVGDRDGLLFNDGQVAMPKGFGDAYKLFAESGWTALNCEPEWGGQ
                     GLPKLVAAPVSEMWKSANHAFSLCPMLTSSAIEALVLSGSDELKQTYLEKMVSGEWTG
                     TMNITEPGAGSDLAAICSRAVPQADGSFRIFGQKIFITYGEHDMADNIIHLMLARTPT
                     APEGVKGISLFLVPKFMVDADGRPGERNDVRCVSIEHKLGIHASPTGVMAFGDKGGAI
                     GFLVGEENRGLEYMFIMMNAARFGVGMEGVSACERAYQRARDYAKDRIQGTDIGVRNG
                     AKVPIIAHPDVRRMLMSMKAQAEASRALAYVVAGAQDKAHHHPEADVRKQNQAFVDLM
                     TPVVKGWSTEIGIEVASTGIQVHGGMGFIEETGAAQHLRDARISSIYEGTTGIQANDL
                     IGRKIARDGGEALKTVIGMMRALDAELAKQSNEHLATIRRRLAAGVNALGKAGEWIVA
                     THASDVHATSAGAVPFLMLFGIVAGGWQMARAALIAQSRIDAGNSDPFYQVKVITARF
                     FADHLLTRADGLADSVTEGATGVMALAEDQF"
BASE COUNT         2332 a         3145 c         3058 g         2237 t
ORIGIN      
        1 caaaggtttt gcctgcgggg aacaaggcct ggcgcaacac ttcgatcaag gccagaccca
       61 tctcgtcaat cgttttgtcc cgcgcctcga cggtcgtgaa tctgcttggg gaaggagacc
      121 tgacccttgc tggtaagtgt cgtcgacatg ggcaggttcc tctcgaattt cgaattccga
      181 attcgtccat tgtaatgccc gaggtaacgc tatgcaatct gcacaaatgc tggccgcagg
      241 ccggcatcga agcgtgtatt ccgctgtatt ccgcttgcga atctgcatcg attcgcggac
      301 aggcccgcac acagcttcgg cgcttgctgc cgcgactcgc ttacaattcc agtaattgcc
      361 atcccaccaa cggtatttca tgtctgcaag gcctgttcgt cgtcatcaga cgttttacct
      421 gcgatggaaa agcgctgttc gccggaacaa gatacgagaa tgacatctgc ccaaccttct
      481 cattttgaaa agctatggct cgccgaatcc ttgcgtcagt tggaaaccga cgcgtggctg
      541 gacgacaagg caatcctgat gcgtctggcg accgagaatg ccgacgagga gtatggcacc
      601 gaggcgttga ttcttgaacg gacccgactg ttggccgaaa agcacaagac cgctgccgcg
      661 atggcaaccg aaattcgcgg agtcagatcg ggtgcaagcg ttgtggctac tgtgttcggt
      721 gtcgctgccg tactgggcgg gatcgcgacg gcctctgtcg ttctcggcaa tcccagcgtc
      781 aatgtgattc tggcgtggtt tgcgcttctc ggtcctcact tcgtgagcct gatcgtgtgg
      841 gggggtggcc ttttgtttgg catgcattcc gcgcgattct ctcttggcag cctgtggagc
      901 gctgcaaccc ggcggtttgc gactggcgga cacacaccgt tgctgttcca ggcagtggcc
      961 agcctgtgcg tgagaacgcg acttccgtac tgggcctatt cactgatcag ccacgcacta
     1021 tggagcctgt tcttcattgg ggcaattctt ggcctttctt atcttctgat gattcgggag
     1081 tacaacttcc gctgggaaac gacaatcctg ccttccgaat tctttgccgg tttcgtcgcc
     1141 ttcttcggct ggttaccgaa actgatcggt tttcagatcc cggaaccgga cgtcgtcatt
     1201 cacctcccag gcggagaaac gtcccgccgg gcatggtcgg cctggctgct gtgcggtgtc
     1261 gttttctacg gcttcctgcc tcgcgtcgtc cttgcactag acggcctgcg acgtttgaag
     1321 agaatcaggc gcggcctcac cctcgacctt gaactgccgt actacgtaag gctcaggcag
     1381 cgccttgatg ccttgcttcg acccgcggta acgattaccg atccggattc gggcgttcct
     1441 gatctggcaa tacaacggag atccgtatca tccggggaca taatcgaccg gggtagcggc
     1501 ttttcgccgc ccttgctgtt cgcccttgag cttcatgatg acgtggtctg gccgccatct
     1561 catcttccag acgggattgt cgtagcagga aatatctcca ccctggatag ccgcgatgct
     1621 ctcctgaaca ggatcgcaca gatcaaacca cgtcgggtcg tcgttgcctg cgaagccaga
     1681 gcaacgccgg atcgaggaac gcttttcctg ttgaacgatg tgtccaatgt cgcggaagcg
     1741 gtgggagtgc ttctcctcaa tcaatcctcg gcgcgtgcgg accgggcgcg gtactggaga
     1801 gaggagcttg cggcagtcgg catcaaggct gaattgatct tccctgagct ctctgagtcg
     1861 ttggtctggc tgaagggatg caatgaataa gtcgagcagc agacccatct cggtcgccgt
     1921 cgtcggacat accaatgcgg gaaagacgtc gcttctgagg acgctcagcc gggacaagaa
     1981 tttcgggaac gtctcggggc aaccggggca gacccggcac gtcgaagcga caagcgtatc
     2041 gattgacgga cgcccggttc tgaagctttt cgacacgcct ggattcgagg actcgatcgc
     2101 gcttcaggag tatttgaaac aatttgagaa ccgcgaaagc cgacgttcct cgcttgctgc
     2161 tttcctgggc agcccggaag ccaaagggag gtttgttcag gaggcaaaga tcctgggggc
     2221 gctgctcggc gatatcgatg cggcgtttta tgtcgttgac acgaccgaga ccccgcatgc
     2281 caagttcagg tccgagctgg atattctcgc ttcatgcgcg gttcccatca tgccggtgct
     2341 caatttcacc ggccatccgg acagccgcct tggcgagtgg aagagtgttc tggctgatcg
     2401 gggattgcac gttcaggttg ccttcgacgt gattgcccct cttgccggca gcgaacggcg
     2461 cctgtatgag cagctcgcat cgctgcttgg tgaacaccgg gcgaaactgg agagtctggt
     2521 tgtcgcgctg gcgaacgagt ccaaagctcg ttcccacgcc agttttcgat cgatcggcga
     2581 cctgcttgtc gatgtggcgg cgtaccgaac acaggtcgtc gaaaagaacg atgatgaaat
     2641 cgcgcgcgaa gtatctgaac tccagtcaaa ggttcgccag cgagaacagc ggtgcgccga
     2701 cgcattgttg tcgatctatc gcttcgaccg gaatgatctt gaggttcgag atttgccttt
     2761 gttgagcgaa cgaatcgagg atgatctgtt taacccggaa gttctgaaac aaaccgctcg
     2821 acgtctcggc aggggcgcag cgatcggcgc tttggtcggc ctcgctgcgg acattggcgt
     2881 tgccggtatt tcgctgggct ctggcgccgt catcggtgga gccgttggtg gcatggtcgt
     2941 cagctcgtgg aagaaatcag cgcgctgggt caaagagaaa atcaacgggt ataccgatct
     3001 gacggtcgat gatgcgaccc tgctggtgtt gctgggaagg cagttgcagt tgctgacctc
     3061 gctgcagggt cgaacacacg cggcgaacga gaggctggtg ctcgatggga atccggcgct
     3121 agcaaaggcg ctggagccgg tcatcgagaa gacgatcatt gcccgtggtc acccggactg
     3181 gtcgaggctc tcggatggtt tcatggacga gagcgatcgt gtcgaagttg tggagtcaat
     3241 cgccgatggc attggagaaa tcgttgttta ggctggcggc gatcagcgcc agatttcagt
     3301 agcggttctc gccgagccgt ttcaaattgt catccagcaa tgccgtcagg cgcggctcgc
     3361 cgttgctggc cacacgaaat actccgaatc gcgcattggc aaacctggac gcatcgcctt
     3421 cctgaaaaaa gaaggcgttg gtggcgacca tcgggcgatg attccgaatg cggtagcgga
     3481 tggcaatctc gtcgataaca ggcagctccg tatccgattg gtttggcaat gcttgcaatg
     3541 gtttcaatgg ttcggactga actcgcagca gcgttgccac gccgcgggaa tcaaggcgaa
     3601 ccacggcata cccatcctcg cgcgccgatt tgcggcgcag ctcattgccg agtgcgtagt
     3661 tgagcgacat gaaatcgccc tgcatcagcg agcgagggtc gatcggcgcc agttccagca
     3721 acacgactcg cccatcggca aggatgcgct cgttcgacca gatgatccat ccggcaccga
     3781 ccaatacaaa gaagagacca aaccatatcc ctgtcgtccg cttcatgcgg gtttctcctg
     3841 cttgcggatc aatgccctta cagtgagcaa cagaattccc ccaagaaaca tcagactggc
     3901 tttgtgcaaa aggatcagct gcaggtcgta gtaaaagcgg ccaacgccga ataccagcat
     3961 ggcgccgccc agccacatca gccagcgatg tccgcgcgca aagccgaata ccagggtcag
     4021 tgctcccatg ccgacagccg gcgcctgcca ggtcataccc aatgcaatgg ccagcaggac
     4081 ggcggcaaac atgttgcgga cgcttttcag ttgcgggcgg gcaattatca gcacaaacgc
     4141 gatattcact gcgctgcaca ataccgcgtc gaaatggaag gctttgtgca ggccgaaaag
     4201 attgtgcgag aaatagccac tcagcgcgta ggcaaagcac agcaaagtca gggcacaggc
     4261 cagggcataa acggattcgc cgaaacgatg cgcctgccag cgcgtttcgt gataccagag
     4321 caggcaaact gcgaaccagt aaatgcctac ccagtagcgc acaagttccc atgagatgga
     4381 catgtctact gccatcacca aggccgcaga accgaccagt gcggcaacga gccggtgcgg
     4441 catccatgcc acccataaca gcatgacggc ttcgtagagg gcgatcatga agaatgaagt
     4501 gctgccttcc cccctgccga acatggttgc gccaacaatc agggcaccgt gcccagccat
     4561 actggcaacc agcaggaact ggcgcagaac cgtgctcccg gtatctacga cacgtcccat
     4621 caacaacccg gtcaatgcgg tcacgatcag cccgatgacg atccagttcg acgcgccttt
     4681 gaccagtggc acggccccca tgccgagaaa cagcagcatg aaaacggcag cgagccagcc
     4741 gccgattact tgcagcgcga gaatccagtg cgaaccaccg gcctcgaacg aggggggtgg
     4801 caaggactcg ccgcgcaggt agccgtcagc ccgtaattca ttcagcacgg gttcaagcat
     4861 cgcctacacc cctggctttc gcccatttgc ttgatgtatg cctcagccag atagcggcgg
     4921 cagcggtcat cgcggccaca accgcaccga tgacaaagaa ggaagtcacg tcgagatact
     4981 tctttccgaa catgcctgag atcaaaccgg cggtaagaac ggcgatgcag gaaagtgcgg
     5041 acgcgacgag cggaacgatg tccttgcggc gtgtgcggta ataccagacc gtcaggaaga
     5101 gccatggcag gtatgcgata ggcgcatagt gcatcagttg ccaaccagtt cgtttgacga
     5161 ggaaccacca gctcacgccg agtccggttg ctgctactgc ggccagaaat acgatgaaac
     5221 gcggcgcata tctcgcccgc atccaggcga tgcgcggcca gtagaattcc cagagcaata
     5281 gcgccaatgc attgagcaac agcggccccc agaatatcgc gtcggaataa atcagaacca
     5341 tccagatatc gaaacgcccg accagaaaca gggccagcga ggtgttaagc agaaccagcc
     5401 agaacaacca gacccagggc gcacgtgcga ttaccaccca gggcagggcg agcaacgccc
     5461 agcccgcgaa aagcaattcg ctatcggcac cggtctggta gatttgtccg attacggcca
     5521 gcagcacccc ggtcagtacg actcccgcgc caagccaggc ttttccagcc gcacgctcaa
     5581 tgccggtata cgccatcatg cttgacgaaa tcaccagcgg tagcactaca agcgatatct
     5641 tcgtgaagcg gtgaagttca ttccagttga aggcaaagaa aaacaccaca ctggccagta
     5701 gcagcaagat acctgcccag agaagcaggc gatctgcaaa gcgtttccag ttggccgcat
     5761 ccggaaacac gccaatacgg tgcagggcga aatccagccc tttcgccgtc aattggccgt
     5821 tgcgtgcgag ggactcaaga tagcgtctgg cagtgagcgg cgtcatgagg tcagcagggc
     5881 tggaaaatgg tcatcttatt atgtattgcg tagctgacaa atagcccggt tgtcttgcgc
     5941 aagacattgc cggtcaagac attgatcaag acgaaggggc ctccattgcc tgcagaatgg
     6001 ccgcggccag caccgcactg gattgtgctc ccggtacggc cagacgatcc tgaatgacaa
     6061 agagtggcac cccgcttatt ccctgtccgc tgataccgtc aaaaagcgat ctgacctgat
     6121 caacgtccat ggtactttcg aggtattcgg tcacgtcgtc catccgatcg ccgcattcag
     6181 cagcgatttc tgccagcgtc gcgatgtcgc cgatatcttc accgcgctga aagtgtgcga
     6241 cgaacagtcg ctcgaccaag gcttcaattt cggccggctg acgcccggtt gactgggcgc
     6301 ggtagatcaa gcggtgtgca tgcagcgtat ttggtcgaat cgaaatcctt tcgaaatcga
     6361 actcgacacc ggtatcacgt ccggcttctg tcacgacctt ctgcaaactg gacacctgct
     6421 ctttcccgcc aaatttggct tcgaggaaag cacggtaggg ctcgccctcg ggcggtgtat
     6481 cggggttgag gaagaacggt agccagttga gccggaagcg ggtttccgga aatttctcgc
     6541 gcacgagtgc gacggcgcca gtcatgcggt gcttgccgat gaaacaccag gggcaaacca
     6601 aatcggaaac gatgtcaatg gatacgttca ttgcgacaag cacgaagttt caaggggtaa
     6661 cacgcgtgct ccgatggcac tcaattgctc tgcctgagcc gggcaagttc cgactgtgcg
     6721 ctttctcctc gcattgcagc ggcaacttcc ttgttgacaa ttgtcttgcc gatgggtgcg
     6781 agagcgatag ccgccagctt gaggtgctgg atgccaaagg ggatgccgat gatggttacg
     6841 aagcatgcgg cggcagaaat cacgtgccca atggccagcc acaccccggc cagcaagaac
     6901 cagacgatat tgccgaagag gccgaggaca cccgtgccaa tgtcactttg tcctgatagt
     6961 tcttccctgc tcactgcttc cttgccgaac ggcaggaagg cgaactgccc gatgacgaag
     7021 caggctttgc cccacggaat tccaatgatc gagacgaaag ccagcagccc tgcgagccac
     7081 cagcccaagc ccatcaacag gccgcccagg atgagccaca ggatattgcc gatcgtactc
     7141 atttcaactc ccgatttcgg acaatcgtgt ccgcaattct tccggaattg gcactgattt
     7201 tccactggcc acatctgtcc agacaatctt ggcatcaccg gttgcgtaca gcgcatcctc
     7261 gccctgcatg cgaatctcat agctcgacat gaagctgctg cggccgggag ggccaaagaa
     7321 catactgacc tctacggtac ccggataggt cagcgggatc aggaaggtgc aactggcgtt
     7381 gatgatgacc ggagccgtgc ctttcggaac gattgagtac ccgaggcttt ccagatattc
     7441 aacgcgagcc tgctccatgt accggaagta gatcgtgttg ttcacgtgat gataggcatc
     7501 catatcgccc cagcgaatgg gagagaggct cttgataaca agcatnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnngctac cccacctgcg cgtttaattc ttgtacgctt
     7681 cttcggatct cttgacgctc tcttccgacg ccttggttgc cgcattcaga ttggccttga
     7741 acgtctccat ctgctccttg ccctgcttgg cttcatctgc tttgagtttt cctactgttg
     7801 cgaccgtact ggcgctttca ataccacagc cggaaagcaa taccagcgtt gagatgacga
     7861 ttgctgagac tgttcgcatg tgtttaccct caaaacaggc cttcgccctg acggatcagg
     7921 attccagcgc aataacgatc gagtaaattt catcgccgga agctgcaatt acttcggcca
     7981 ccgtactctg gtcaagacca agggtggccg cactgtcggc catgggagtc acgcgggcgt
     8041 cgagttcatc gaatggatcc gggcgcggcg agacggcacc cgcgataaag aggaggtcac
     8101 tgagcgaggc aggaggcatc gacccactga attgcttcga ggcaagtacg gcatccacaa
     8161 tggatgcagg cagcttcagc tcgttgagga cgatttccgt agcatgcccg gccagatcat
     8221 tgatggtttc agccagtaca acggtatctt caagcagcgc aggaaaatcg gcaacgcgcg
     8281 agagcagata gaaacgtccc agatcatgga cgatgccggc aaacatgact tcgtcggcgt
     8341 tgagtttggt gagttttcgg gccagaacgt aagacaacgc ggccacatgc acactgcgtt
     8401 cccacaatcg gttggacaga tcgcggcagc cactgtggcg ctgcgactga cgcagctgat
     8461 ccatgatcag caccatcgcc agtcccttga tcggatccat gccgacgcgc atcaccgcgt
     8521 gtctgacgtc gcgcaccgtc tgattggtcg gattcagggc aacggaattg gcgagtcgaa
     8581 tcacctttgc ggacagcaga ggctctgcga cgacaatctt tgccagcgcg gccatgccga
     8641 ggtctgggtt ttccactgcc ttcagaaccc gccgactggc atccaggctg gtcgggaagc
     8701 tgagttcgcc tatgtttgcg gtaccgaccg cctggcggag agattcggcg gcttcatgta
     8761 tcgtttgact cacgctgcgc tccttgtcaa tttcagcaac ccaccgcatt ctaaactctt
     8821 gctgagcgtt tttgagcaat caaagaggat ttcctccgac aaacccggcg aggtgccctg
     8881 aaggggaact gtagagtatg gcgaaatcgc cagagtttct cagcattctc caaacctctg
     8941 cgttcaatgc ggcgaaagtg ctccctcaga actggtcctc cgccagtgcc atcacgccag
     9001 tcgcgccctc ggtgaccgaa tctgccaaac cgtcggcccg cgtcagcaag tggtcggcga
     9061 agaaacgggc agtgatgact ttgacctggt aaaacggatc gctgttgccc gcgtcaatcc
     9121 ttgattgtgc gatgagcgct gcgcgcgcca tctgccaccc tccggcaacg attccgaaga
     9181 gcatgagaaa ggggacagcg cccgctgaag tcgcatgcac gtcggaagca tgggtggcga
     9241 caatccactc cccggccttg cctagagcat tgacgccggc agccaaacgg cggcgaattg
     9301 tggctagatg ctcgttgctt tgcttggcca gctcggcatc cagtgcccgc atcataccga
     9361 tgacggtttt gagcgcttcg ccgccgtcgc gagcgatttt tcgaccgatc aaatcgttgg
     9421 cctgaatacc cgtcgtgcct tcgtaaatcg aggaaattcg ggcatcacgc agatgctgtg
     9481 cagcgccagt ttcctcgatg aagcccatcc cgccgtgcac ctgtatcccg gttgacgcga
     9541 cttcgattcc aatctcggta ctccagccct tgaccaccgg cgtcatcagg tcgacgaagg
     9601 cctgattctg cttgcgcaca tcggcttccg gatggtgatg cgccttgtct tgtgctcccg
     9661 ccacgacgta ggccagtgcg cggctcgctt cagcctgtgc tttcatgctc atgagcatgc
     9721 gacgtacgtc aggatgcgca atgatcggga ccttggcgcc attccgcaca ccgatgtccg
     9781 taccctgaat gcgatccttg gcgtaatcgc gcgcacgttg ataggcgcgt tcacatgcgg
     9841 acacgccctc catgccgaca ccgaaacgtg cggcgttcat catgatgaac atatactcaa
     9901 gcccgcggtt ttcctcaccc accagaaaac cgatggcccc gcctttgtcg ccaaaggcca
     9961 tcacgcctgt cgggctggcg tggattccga gcttgtgttc gatggacacg cagcgcacgt
    10021 cattgcgttc gcccgggcgg ccatctgcgt caaccatgaa ctttggtacg aggaagaggg
    10081 aaatgccctt gacgccctcg ggtgccgtcg gggttcgtgc cagcatcagg tggatgatgt
    10141 tgtccgccat atcgtgctca ccgtaggtga taaatatctt ctggccgaag atcctgaaac
    10201 tgccatcggc ttgtggcact gcgcgcgaac atatggccgc gaggtcggat ccggcgcccg
    10261 gctcggtgat gttcatcgta cccgtccact cgccgctgac cattttttcg aggtacgtct
    10321 gcttgagttc atccgacccg gaaagcacca gcgcttcgat ggcactgctg gtcagcatcg
    10381 gacaaagcga aaaggcgtgg ttggccgatt tccacatctc ggacacgggt gctgcaacga
    10441 gcttgggcag gccttgtcct ccccattccg gctcgcagtt gagcgctgtc cagccgcttt
    10501 cggcaaacaa cttgtaggcg tcaccgaaac ccttgggcat ggccacctgc ccgtcattga
    10561 acaacaaccc atcgcggtcg ccaacaccgt tgagcggtga aagtacacct tcggcaaatt
    10621 tggctgcttc ttccagaacg gcgtcaacaa catcagtcga tgcttcctca cagccgggca
    10681 attgcgccac gtcctcaagc ccggccaatt ccttcaacac aaactgcatg tcgcggagcg
    10741 gggcacggta ttcgctcatt tcactctcca gtcagaataa agacaactcg ccacttgaat
    10801 tggcaccatc gcggaatcgt cccctgctca ccccagcgcg ttggtcaatg cgggagccag
    10861 ctcgaacaga tc
//
LOCUS       DAORPM010000058        10801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1377_length_10801_cov_9.160525, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000058 DAORPM010000000
VERSION     DAORPM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1377_length_10801_cov_9.160525"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PLD53_04375"
     CDS             complement(<1..806)
                     /locus_tag="PLD53_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRC60175.1"
                     /translation="MSQQNKTDEGKTMPDPAELIKSYSEVAKRASQMMAKYIDKKASE
                     GISMPADEMGIAKAFSDLASRLLADPQKLMEAQMKMMRDYVSLWQYSASKMMGSTSVA
                     PVAVPAKSDKRFKDDEWESNFLFDFIKQSYLITSGHMNNIVAETEGLDESTHRKVDFF
                     TRQYIDAMSPTNFAMSNPEVVRETMNSGGKNLISGFDNLLRDLEAGDGKLKIRMTDPD
                     AFELGKNVATTPGKVVFQNEMFQLIQYDPATPQQFKKPLLIVPPWINKFY"
     gene            complement(1070..1918)
                     /locus_tag="PLD53_04380"
     CDS             complement(1070..1918)
                     /locus_tag="PLD53_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRC60176.1"
                     /translation="MKLKGLGRGLDALLAGNEASGKEQQRALPVGDLQPGKYQPRTRM
                     DSAALEELAASIRVQGLMQPILVRPVDNASGEDRYEIIAGERRWRAAQMAGLHEVPTL
                     IREIPDESALAMALIENIQRENLNPLEEAQGLQRLIDEFSMTHQQAADAVGRSRPAAS
                     NLLRLLQLAEPVQELLMRGEIEMGHARALLPLSGVLQIQLAQRVVQKGLSVRDAERMA
                     QHALKPPREVEARKPDRDVQRLQEELSDFLGAQVEIVTNKKGAGKIQIEFGNLDQLEG
                     ILQRMR"
     gene            complement(1915..2715)
                     /locus_tag="PLD53_04385"
     CDS             complement(1915..2715)
                     /locus_tag="PLD53_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC60177.1"
                     /translation="MAKILAVTNQKGGVGKTTTAVNLSACLAVLGQRVLMIDLDPQGN
                     ATTGCGVAKRVALPTVYQILIGRSSLSETCIRTDFGFDILPANRELAGAEVELIEMGG
                     REFRLRDALVPVQDEYDFILMDCPPALNMLTVNGLVAADAVMIPMQCEYYALEGLTDL
                     VATLKKVRANLNSRLEIEGLLRTLFDSRSTLTQQVSSELVDHFGSKVYRTIIPRNVRL
                     AEAPSYGKPVIAFDRLSKGAQAYRALAQEMLDRRDGTVRSDAAQGAEA"
     gene            complement(2717..3400)
                     /gene="rsmG"
                     /locus_tag="PLD53_04390"
     CDS             complement(2717..3400)
                     /gene="rsmG"
                     /locus_tag="PLD53_04390"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRC60178.1"
                     /translation="MRHARLPEVRESTRQAVQLGHGLDAMGIEVPGAVQASLLAFAEL
                     LCKWNRTYNLTALNDPDKVLSHHLLDSLAVLPYVPPGRLLDVGSGGGMPGIPLAIARP
                     DLHVTLLDSNSKKTAFLQQAMIELTLPNISVHCGRVEQYHPSIGFAAIVSRAFSELAD
                     FVGLTRHLLGAGGRWLAMKGVWPHDEIERLPADVRLDVVHRLQVPGVEGERHLVVMRS
                     ADAYSSGGS"
     gene            complement(3378..5297)
                     /gene="mnmG"
                     /locus_tag="PLD53_04395"
     CDS             complement(3378..5297)
                     /gene="mnmG"
                     /locus_tag="PLD53_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRC60179.1"
                     /translation="MQFPDRFDVIVIGGGHAGTEAALASARMGAKTLLLTHNLDTLGA
                     MSCNPSIGGIGKGHLVKEVDALDGAMAAATDEAGIQFRILNSSKGPAVRATRAQADRV
                     LYRQAIRRRLENQPNLTLFAQACDDLLVEGDRVTGAVTQLGVRFAARAVVLTAGTFLN
                     GLIHVGMSNTTGGRMGDMPSISLAARLRELQLPVGRLKTGTPPRLDGNTIDFAQLEQQ
                     HSDNPLPVFSLLGRASQHPQQLPCWITNTNERTHDIIRANLDRSPMYAGVIEGVGPRY
                     CPSIEDKIHRFADKNSHNVFLEPEGLTTNEIYPNGISTSLPFDVQLELVRSMRGLENC
                     HILRPGYAIEYDYFDPCSLHASLETKAIGGLFFAGQINGTTGYEEAAAQGLLAGANAA
                     LQVKGKEAWTPRRDEAYLGVLVDDLITRGVSEPYRMFTSRAEYRLSLREDNADMRLTE
                     HGRRLGLVGDVRWSAFCTKREAVAREQERLRSTWVHPSKVSADETTRVLGKPIEREYT
                     LYDLLRRPDVSYSTLMGLQWNEPSDASPIDAGLSDALVIEQVEIQAKYQGYVDRQLEE
                     VARNLSNEETRLPVDFDYSRIGGLSNEIRQKLIRHRPETIGQASRIQGVTPAAISILL
                     VSLRRGALDASRKIA"
     gene            5483..7207
                     /locus_tag="PLD53_04400"
     CDS             5483..7207
                     /locus_tag="PLD53_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC60180.1"
                     /translation="MGTSDLNDIADIQHRLGKELARMRVLLVDRHASARNSMRIILSS
                     LGITAVHNAGTSAEVLRQVKAYTFDIILSDYHLDDGRDGQQLLEELRHKNLISLSTVY
                     MVVTAERAYQNVVSVAELAPDDYLIKPFTADQFHSRLIKTIYKKHFFEKVFGHLDNGA
                     YAEALAACDSLIGKEDAYLYDILRFKGEILNVLGRHEEAKAVYQQVLDHAMVPWARMG
                     LAIALRGMDELAEAEEMGASVIDDFPEYLAAYDFVASVREEMGKLAEAQQVLQKATVI
                     SPNNSVRQRMVGDVAVRNDDLDAAERAYGKVLERHQGSSLRVLDDYTNLTRVMLDKGH
                     TEGAKKVTQDLRRDWRGNKQGELAALIMDSLCCEQEGEPAKAKQALEKALELRSSLGD
                     SDQNEFSQKIAVDLAHACLASGDEKMAQEILSKVAAENHEDRSMIAQIQGVFSKTGHE
                     EAGQSMLAQVGKEIVELNNRGVLAARSGDIETSVQMLIEAAERVPNMQFLVNATKAIF
                     TLLDRKGWDEEMGDRGVRYLQMAQSKDPRNAKVISARELYHKVARKFGIEVVPIGGVR
                     TAGEKSGG"
     gene            complement(7238..8308)
                     /gene="mutY"
                     /locus_tag="PLD53_04405"
     CDS             complement(7238..8308)
                     /gene="mutY"
                     /locus_tag="PLD53_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRC60181.1"
                     /translation="MRENRSARVFSTRLIDWQRHHGRHDLPWQNTRDAYRIWLSEIML
                     QQTQVGTVIPYYARFVECFPTIEALALAPLATVLELWAGLGYYARARNLHRCAQAIVA
                     HYQAEFPGDLQSLCELPGIGRSTAAAISVFAFGHRAPILDGNVKRVLARCFGIDGFPG
                     SAATENVLWSLAETLLPAKHIEAYTQGLMDLGSTVCTRRKPSCEACPLHAMCIARLDG
                     RQAELPVARPVKVLPERDTTLLLLTDGNRFLLERRPPVGIWGGLLSLPEGTGAGASEF
                     ARRHGCRLLEAQVLPSLKHTFSHFRLNILPLLCTVKATPHLVAEAGWQWLEHGEVDTA
                     ALPTPIMRLLKTIRKSIPPTRD"
     gene            complement(8305..8460)
                     /locus_tag="PLD53_04410"
     CDS             complement(8305..8460)
                     /locus_tag="PLD53_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60182.1"
                     /translation="MSMQCLDCLRNVSLAQEDEMYDVPIIAPPGAAVEELEQRLEQTG
                     GAFPVCP"
     gene            8541..9134
                     /locus_tag="PLD53_04415"
     CDS             8541..9134
                     /locus_tag="PLD53_04415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRC60183.1"
                     /translation="MTLSTSTENCPDLLTSGIDLPCATCDTGRSLAALGFCCRDFRST
                     LGCFATGVTVVTALAPDGEPIGLTINSFNSVSLDPPLILWSLSLGSPMLNAFQRASHY
                     SVNVLSVDQQNLSDRFAERRVDHFVDLPQRAGLGGAPLIEGCCAWFECANQTQYPGGD
                     HLIFVGLVERFARGKAESPLLFHDARYCRIDASRTTD"
     gene            9198..9641
                     /locus_tag="PLD53_04420"
     CDS             9198..9641
                     /locus_tag="PLD53_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRC60184.1"
                     /translation="MANKQHSSSMILTITVVVAIVLMIIIWPLSMLGKGPSATGSGDE
                     VELRILPVARIEMKKAVVVSDGKPRDGATIYNASCAACHASGAAGAPKTGDKAAWAPR
                     IAAGTATLLKNATNGKGAMPPRGGASDLSDEELKAAVNYLVGKAK"
     gene            complement(9771..10130)
                     /locus_tag="PLD53_04425"
     CDS             complement(9771..10130)
                     /locus_tag="PLD53_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60185.1"
                     /translation="MLKNIIALAIAAAFGTAAYAQAPAAAPAPAKPVAATVAPAAATT
                     APAAAATASKPEMKVEEKKAEAAPVATAEEKKVEAKTKTKTVKGKAAAKPEVMPAEKK
                     VEAGHAATPATAAPMTK"
     gene            complement(10309..>10801)
                     /locus_tag="PLD53_04430"
     CDS             complement(10309..>10801)
                     /locus_tag="PLD53_04430"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRC60186.1"
                     /translation="EEDGKSLIELTQTIALINLLDKQDGGGRPDAVQLSTLHAAKGLE
                     FKHVFLIGVEEGMLPHRESQDADKIEEERRLMYVGITRAQRSLHLSYCEKRKQGRELI
                     PCEASRFIAEMGKDDLRFSGGKTDSAPDKAGGAARLSAMKAMLAGNPGAPREKLDSAE
                     KPI"
BASE COUNT         2117 a         3242 c         3212 g         2230 t
ORIGIN      
        1 atgtagaact tgttgatcca cggcggcacg atcagcagcg gcttcttgaa ttgctgcggt
       61 gttgccggat cgtactggat cagctggaac atttcgttct ggaagacgac cttgcccggt
      121 gtggtggcga cattcttgcc gagctcgaag gcgtcggggt cggtcatgcg gatcttcagc
      181 ttgccgtcac cggcctccag gtcacgcaga agattgtcga atccactgat caggttcttc
      241 ccgccgctgt tcatcgtctc gcggacgact tccggattgc tcattgcgaa gttcgtcgga
      301 gacatggcgt cgatgtactg acgggtgaag aaatcgacct tccgatgcgt cgactcgtca
      361 aggccttccg tctcggcaac gatattattc atgtggccgg acgtgatcag gtaggactgc
      421 ttgatgaaat cgaacaggaa gttgctctcc cattcatcgt ccttgaagcg cttgtccgac
      481 tttgcgggaa cggcaacggg cgctaccgat gtggagccca tcatcttcga tgccgagtac
      541 tgccaaagcg aaacatagtc gcgcatcatt ttcatttgcg cttccatcag tttttgaggg
      601 tcggccagca ggcgtgaagc gagatccgag aacgccttgg caattcccat ctcgtccgcc
      661 ggcatcgaga ttccctcgct cgccttcttg tcgatgtact tggccatcat ttgcgaagcg
      721 cgcttggcca cttcggaata gctcttaata agctctgcag gatcaggcat ggtcttgcct
      781 tcgtccgttt tgttctgctg cgacatcgaa taacctcctt gaactggtac tcaataaagt
      841 gatagggacg ccttacaata cacggaggcg gatggataaa ccgcaagaaa ccttaaactt
      901 tgaccgtgaa gaacgacctg ttcggcacta aacctgaata atatagcatt cggggcgcac
      961 tttggcaccg ctttagcagg aaaatatcct gcaatccggt tcgtttgcaa cccggagggc
     1021 cttgcagaac ggccagcagg ggttctgctc gaaggcgtcc ggaagccctt tagcgcatcc
     1081 gctgcaggat gccttcgagc tgatcgagat tgccgaattc aatctggatt ttgccggcac
     1141 ctttcttgtt ggtaacgatt tcgacctgcg caccgagaaa atccgatagc tcttcctgca
     1201 aacgctgcac gtcgcgatcc ggcttccggg cttcgacctc tcgcggtggc ttgagtgcgt
     1261 gttgtgccat ccgctcggca tcgcgtaccg acaaaccttt ttgcaccacc cgctgggcaa
     1321 gctgaatctg cagcacgccg gacaaaggca acaaggcacg ggcatggccc atctcgatct
     1381 ctccgcgcat cagcaattcc tgcaccggct ccgccaactg caacagacgc agcagatttg
     1441 acgcggcggg ccgcgaacga ccgaccgcat cggctgcctg ctggtgcgtc atcgaaaatt
     1501 cgtcgatcag ccgctgcagc ccctgtgcct cttccagcgg gttgagattt tcgcgctgga
     1561 tgttttcgat cagagccatg gccagcgccg attcatccgg gatttcgcga atcagggttg
     1621 gaacttcgtg cagcccggcc atttgcgctg cacgccagcg gcgctcaccg gcgatgattt
     1681 catagcggtc ttcgcccgac gcattgtcga ccggccggac gaggatcggc tgcatcaaac
     1741 cctgaacgcg aatcgaggcg gccagttctt caagcgctgc gctgtccatc cgggtgcgcg
     1801 gctgatactt gccgggctgc agatcgccga ccggaagcgc gcgttgctgt tccttgcctg
     1861 acgcttcgtt gccagccagc agcgcatcga gtccgcgacc gagtcctttc agtttcatgc
     1921 ttctgctcct tgtgcggcat cggatcgcac ggtgccatcc cgccgatcca gcatttcctg
     1981 cgccaacgcc ctataagcct gcgcgccctt cgagagccgg tcaaaggcga tcaccggctt
     2041 gccgtaggag ggtgcttctg ccagccgtac attgcgcgga atgatggtgc gatagacctt
     2101 gcttccgaag tgatcaacca gctcgctgga gacctgctgc gtcagcgtgg aacgtgaatc
     2161 gaacagggtg cgcaacaagc cttcgatttc gagcctggaa ttgagattgg cgcgtacctt
     2221 cttcagcgtg gcgacgaggt cggtcagtcc ctccagcgcg tagtattcgc attgcatcgg
     2281 tatcatcacg gcatcggcag cgaccagccc gttaacggtc agcatattga gcgccggcgg
     2341 acagtccatc aggatgaagt cgtattcatc ctgaaccgga accagggcat cacgcagccg
     2401 gaactcgcga ccgcccatct cgatcagctc gacctccgca cctgcgagtt cacgattggc
     2461 gggcaggatg tcgaaaccga aatccgtccg gatgcacgtt tcgctcaggg acgagcggcc
     2521 gatcaggatc tggtagaccg tcggcaaggc aacgcgcttg gcaacgccgc aacccgtggt
     2581 tgcattgccc tgcggatcaa ggtcgatcat gagtacgcgc tgtccaagga cggcgaggca
     2641 ggcggacagg ttgaccgccg tcgtcgtttt gccgacgccg cctttctggt tggtcacggc
     2701 cagtatcttt gccaactcaa ctcccccctg aactgtaagc gtcggcactg cgcatgacga
     2761 ccagatgacg ctccccctca actcccggta cctgcaggcg atgaacgacg tcgagccgca
     2821 catcggcggg caggcgctca atttcatcgt gcggccagac tcccttcatg gccagccacc
     2881 gtcctcccgc gcccagcaga tgccttgtca aaccgacaaa atcagccagc tcggagaagg
     2941 cacgcgacac gatcgcggca aagccgatcg acggatgata ctgttcgact cgtccgcagt
     3001 gaacggaaat attgggcaag gtcagttcaa tcatggcctg ctgcagaaaa gcggtcttct
     3061 tcgaattgct gtcaagcaag gttacgtgta aatccggccg ggcgatagcc aggggaattc
     3121 ccggcatgcc gccgccgcta cctacatcca gaagcctgcc cggcggaacg taaggcagca
     3181 cggctagcga gtcgagcaga tgatggctga gcaccttgtc cgggtcgttc agcgccgtca
     3241 ggttgtaggt tctattccac ttgcacagca gttccgcaaa cgccagcagg ctggcctgca
     3301 ccgcaccggg cacctcgata cccatcgcgt ccaggccgtg cccgagttgc accgcctgac
     3361 gcgttgactc acgcacttca ggcaatcttg cgtgacgcat ccaaggcgcc gcgcctgaga
     3421 gaaacgagca agatcgagat cgccgccggt gtcacgccct gaatccgcga ggcctggcca
     3481 attgtctccg gcctgtgccg tatcagcttt tgtcggatct cgttcgacag gccgccaatc
     3541 cggctgtagt cgaaatccac tggtaaacgt gtttcttcat tggacagatt tcgcgcaacc
     3601 tcttcgagct gacggtcgac atagccctgg tacttggcct ggatttcaac ctgttcgatg
     3661 accaatgcat ctgacaatcc ggcatcgatg ggcgacgcat ccgacggctc gttccattgg
     3721 aggcccatca aggtcgaata gctcacgtcc gggcgccgca acagatcgta caacgtgtat
     3781 tcccgttcga tcggcttgcc cagtacgcgg gttgtttcat cggccgacac tttggacgga
     3841 tgcacccagg tcgaccggag acgctcctgc tcgcgcgcaa ccgcctcgcg tttcgtgcag
     3901 aaggccgacc agcgcacgtc gccgaccaga ccaagccgtc gtccgtgctc ggtcaggcgc
     3961 atgtcggcgt tatcctcacg caaggaaagt cggtactcgg cgcgactggt aaacatgcga
     4021 tagggttcgg acacgcctcg cgtgatcagg tcgtcgacca gtacgccaag ataggcctcg
     4081 tcgcggcgcg gcgtccaggc ttcctttcct ttgacctgca gggccgcatt ggcaccggcc
     4141 agcaggccct gcgccgccgc ttcttcatag cctgtcgttc cattgatctg gccggcaaaa
     4201 aagaggccgc ctatggcctt ggtttcaagc gaagcgtgca ggctgcaagg atcaaagtag
     4261 tcgtattcga tggcgtagcc ggggcgcaat atgtggcaat tttccaggcc gcgcatcgag
     4321 cgcaccagtt ccagctgaac gtcgaagggc aaactcgttg aaatcccgtt cgggtagatt
     4381 tcgttggtcg tcaacccctc cggctcaaga aagacgttgt ggctgttctt gtcggcaaag
     4441 cggtggatct tgtcttcgat cgacggacag tagcgcggcc cgacgccttc gatcacgccg
     4501 gcatacatcg gcgagcgatc cagattggcg cgaatgatgt cgtgcgtccg ctcgttggta
     4561 ttggtgatcc agcagggcaa ctgctgcggg tgctggctgg ccctgccgag aagcgaaaat
     4621 accggcaagg ggttgtccga gtgctgctgc tcgagctgcg cgaaatcgat ggtattgcca
     4681 tcaaggcgcg gcggcgtgcc tgttttcagg cgtccaacgg ggagctgcaa ttcgcgcaag
     4741 cgcgcggcca gcgaaatcga tggcatgtcg cccatgcgcc cgccggtggt attggacatg
     4801 ccgacgtgaa tcaggccgtt gagaaaggtg ccggcggtca gcacgacggc gcgtgccgca
     4861 aaacgcaccc cgagctgtgt aaccgccccg gtcacccgat cgccctcgac gagcagatcg
     4921 tcgcaggcct gggcaaacag ggtcagattc ggttgattct ccagtcggcg gcggatcgcc
     4981 tgccggtaca gtacccgatc ggcctgcgcg cgggtcgcgc gcacggctgg acccttgctc
     5041 gaattgagga tgcgaaactg gatgcccgct tcgtcggttg cggcggccat tgcaccgtcc
     5101 agcgcgtcga cctccttgac gagatgcccc ttgccgatgc cgccgatcga agggttgcag
     5161 ctcatggcgc ccagcgtgtc caggttgtgc gtgagcagca gcgtcttcgc gcccatgcgc
     5221 gcgctggcaa gcgcggcctc ggtgccggca tgtccaccgc caataacgat cacatcgaaa
     5281 cgatcaggaa actgcatgag aagtccgcga aatccgggaa cgccgaaggg atgcggattc
     5341 tacgccaatt cgctgaaaaa taacaggttt cggccgattc atgagcttct tcgccgcatt
     5401 ttatcgagtc gcgccatcgc gcaacagcgt atggtatgac gttccgcttg cccgccgaag
     5461 cacagtcgcg taaggtgctt tcatgggtac atccgatctc aacgacattg ccgatattca
     5521 gcacaggctg gggaaggaac tggcacgcat gcgggtgttg ctggtcgatc ggcacgccag
     5581 cgcgcgcaat tccatgcgca tcattctgtc gagcctcggg atcaccgccg tccataacgc
     5641 aggaacttcg gcggaagtat tgcggcaagt caaagcgtat acgtttgaca tcattctctc
     5701 cgactaccac ctcgatgacg gccgcgacgg ccagcagctg ctcgaggagt tgcgtcacaa
     5761 gaatctgatt tcgctatcga ccgtatacat ggtcgttacg gccgaacgcg cctatcagaa
     5821 cgtggtatcc gttgccgaac tcgcgccgga cgattacctg atcaaacctt ttaccgccga
     5881 tcagttccac agtcgtctga tcaagaccat ctacaagaag cacttcttcg aaaaggtatt
     5941 cgggcatctg gacaatggcg cttatgccga agcgctggcg gcttgcgaca gcctgatcgg
     6001 caaggaggac gcgtatcttt atgacatcct gcgtttcaag ggcgagattc tgaacgtact
     6061 cggtcggcac gaggaagcca aggcggtcta tcagcaggtg cttgaccatg ccatggtgcc
     6121 atgggcgcgc atgggtctgg ccattgcgtt gcgcggtatg gacgagctgg ccgaggccga
     6181 ggaaatgggt gcgtcggtga tcgacgactt tcccgagtat ctggcggcgt atgatttcgt
     6241 cgccagcgtt cgcgaagaga tgggaaagct cgccgaagcc cagcaagtac tgcagaaggc
     6301 cacggtgata tcgcccaaca attcggtgcg tcaacgcatg gtcggcgacg tggccgtccg
     6361 caacgatgat ctcgatgccg ccgaacgcgc ttacggcaag gtactcgaac gccatcaagg
     6421 ctcgtcgctc agggtcctcg acgactacac caatctgacg cgcgtcatgc tcgacaaggg
     6481 acacaccgag ggcgccaaga aagttaccca ggatctccgt cgcgactggc gtggcaacaa
     6541 gcagggcgag cttgccgcgt tgatcatgga cagtctgtgc tgcgaacagg aaggcgagcc
     6601 ggccaaggcc aaacaggcgc tcgaaaaggc gcttgaacta cgcagttcgc ttggcgattc
     6661 cgaccagaac gagttctcgc agaagatcgc cgtcgatctg gcccacgcct gcctggcgtc
     6721 tggcgatgaa aagatggcgc aggagatcct gagcaaggtc gctgccgaaa accacgaaga
     6781 ccgcagcatg atcgcccaga ttcagggcgt cttctccaag acaggccacg aagaagcagg
     6841 ccagtcgatg ctcgcccagg tcggcaagga gatcgtcgaa ctcaacaatc gaggcgttct
     6901 ggccgcgcgc agcggagata tcgaaacctc agtgcagatg ctgatcgaag cagccgagcg
     6961 tgttcccaat atgcagttcc tggtcaacgc caccaaggcg atcttcaccc tgcttgaccg
     7021 aaagggctgg gacgaggaga tgggagatcg cggcgtacgc tacctgcaaa tggcccagtc
     7081 gaaggatccg cgaaacgcca aggtcatttc agcgcgcgag ctctatcaca aggtggctcg
     7141 aaagttcgga atagaagtcg tcccgatcgg cggcgttcgc actgccggcg aaaagagcgg
     7201 cgggtaggga atcgggtttt cgatcgccgg ctgctgctca atcgcgggtc ggcggaatgc
     7261 tttttcgtat cgtcttgagc aggcgcatga tcggcgtcgg cagcgccgcg gtgtcgacct
     7321 cgccgtgctc caaccattgc caccccgctt cagcaacaag atgaggcgtc gctttaacgg
     7381 tgcatagcaa gggcaggata ttcaggcgga aatggctgaa tgtatgcttg agcgatggca
     7441 acacctgcgc ttcgagcaga cgacagccgt ggcgccgcgc aaattcgctg gctcctgccc
     7501 cagtcccctc gggcagggat agcagaccgc cccagatccc gaccggcggc ctgcgttcca
     7561 gtaagaaacg attaccgtcg gtaagcagca ataaggtcgt atcgcgttcg ggtagcactt
     7621 tcacaggtct ggcgaccggc aattcggctt gccggccatc gagccgggca atgcacatcg
     7681 catgcaacgg gcaggcttcg cacgacggct tgcggcgtgt gcacaccgtt gagccgaggt
     7741 ccatcagacc ctgggtgtag gcttcgatat gctttgctgg aagcagggtt tcggccagtg
     7801 accacagcac gttttccgtt gctgccgacc cgggaaatcc atcgatgcca aagcagcgcg
     7861 ccagtacccg cttgacattg ccatcaagga tcggtgcgcg atggccgaag gcaaagacgc
     7921 tgattgccgc cgccgtcgaa cgcccaattc ccggcaactc gcagagtgac tgcaggtcgc
     7981 ccggaaactc ggcctggtag tgggcaacga tggcttgcgc gcagcgatgc agattgcgtg
     8041 ccctggcgta ataacccagc ccggcccata gttcgagcac tgtggcaagc ggcgccaggg
     8101 ccaaagcctc gatcgtcggg aagcactcaa caaagcgcgc gtaatacgga atcaccgtgc
     8161 cgacctgggt ttgctgcagc atgatttcgg agagccagat gcggtatgcg tcgcgcgtat
     8221 tctgccaagg cagatcgtgg cggccatgat ggcgttgcca gtcgatcagc cgggtggaaa
     8281 aaacccttgc ggatcggttt tccctcatgg gcacactgga aaagcgccgc ctgtttgctc
     8341 aagtctctgc tcaagttcct cgacagccgc cccaggcggg gcgataatcg ggacgtcgta
     8401 catctcgtct tcctgcgcca gactgacatt ccggaggcaa tcgaggcatt gcattgacat
     8461 aacgaactcc tgaacctctc tgatcgccgc agatcttagc atgcccaccc cgcccattct
     8521 gaccccttcc tgattccctg atgacgctgt caacctccac cgaaaactgc cctgatttgc
     8581 tgacgtcagg aattgatctc ccctgtgcga cttgcgatac cggacgttcg ctggccgccc
     8641 tgggcttttg ctgtcgcgac ttccgcagca cgctcggctg ctttgccacg ggtgtgaccg
     8701 tcgttaccgc actggcgccg gatggcgagc cgattggact gacgatcaat tcattcaatt
     8761 cggtatcgct ggacccgccg ctcattctct ggagcctgtc cctgggttcg cccatgctga
     8821 acgcgttcca gcgggccagc cattattcgg tcaatgttct ttcggttgac cagcagaatc
     8881 tctcggaccg ttttgcggag cgccgcgtcg atcacttcgt cgacctgccg caacgcgcgg
     8941 ggctgggcgg cgcgccgctg atcgaaggtt gctgcgcctg gttcgaatgc gccaaccaga
     9001 cgcagtatcc gggtggcgat catctcatct tcgtcggact tgtcgagcga tttgcccgcg
     9061 gcaaagccga gtccccgttg ctctttcacg acgcccgtta ttgccggata gacgcctccc
     9121 gaacgacgga ttgaatcgga tcgggtaaac tgcgggctga cgtttcagtc atcaactgca
     9181 gctaaccggg gtttcacatg gcaaacaaac aacattcttc ttcgatgatc ctgacgatca
     9241 cggtcgtcgt ggcaatcgtc ctgatgatca tcatctggcc actgtcaatg ctgggcaagg
     9301 gaccgtcggc tacaggcagt ggcgacgaag tcgagttgcg tatcctgccg gtggcgcgaa
     9361 tcgagatgaa aaaagcagtc gtagtatccg acggcaaacc gcgcgatggc gccacgatct
     9421 acaacgcgtc ctgcgcggct tgccatgcca gcggagctgc aggagcaccg aaaacgggtg
     9481 acaaggcagc ctgggcgccg cgcattgcgg cgggtacagc gaccctgctc aagaacgcga
     9541 ccaatggcaa aggcgcgatg ccgccacgag gcggagcctc tgatcttagc gatgaggagc
     9601 tgaaagccgc ggtgaattac ctggtcggca aggccaagta ggcgagtccg gctacggaat
     9661 ccagcttcta cttcgcggag taaaaagagg gggaggcctt tgtaaggcct ccccctttat
     9721 cctgacccga gggggtatcg gatcaggttg ctgctcaagg tattcgctgc ttacttggtc
     9781 atcggagctg cggttgcagg tgtggcggcg tgtccggcct cgaccttctt ctctgcgggc
     9841 atcacttcgg gcttggcggc tgccttgccc ttgacggtct tggttttggt cttggcttcg
     9901 actttctttt cttctgccgt ggctaccggg gcggcttcag ccttcttttc ttcaactttc
     9961 atttccggtt tgctggcggt agccgctgcg gcaggggctg tggttgctgc ggcaggagct
    10021 acagtcgccg caaccggctt ggctggggct ggtgcagcgg ccggagcctg ggcgtaggcg
    10081 gcagttccga aagcggcggc aatggcaagt gcaatgatgt tcttaagcat gtttatctcc
    10141 tgatgatggt tgggttcttc tatcggtcgc cacgttttca agattcgttg cggccttgca
    10201 gacatcaacg cgaccggttg ccatagggtt gtcaatgatt gcgtaacgcc gcgtaacgct
    10261 ttgtgacagc gtcatcgcgc ggctggagtc aggctgaacg gatttgtgtc agataggctt
    10321 ttcggcggag tccagcttct cgcggggcgc tcccggattg ccggccagca tggccttcat
    10381 ggccgacagg cgcgcagcac cgccggcctt gtcgggcgca ctgtccgtct ttccgcccga
    10441 gaaacgcaga tcgtccttgc ccatctcggc gatgaagcgt gaggcttcgc atggtatgag
    10501 ttcgcgcccc tgtttgcgct tctcgcagta gctcaggtgc agcgagcgct gcgcgcgggt
    10561 gatgccgacg tacatcaggc ggcgctcttc ctcgatcttg tccgcatcct gcgattcacg
    10621 atgcggcagc atgccttcct cgacgccgat caggaaaacg tgcttgaact ccagtccctt
    10681 ggccgcgtgc agggtagaca actgaaccgc atcgggccgg ccgccgccgt cctgcttgtc
    10741 cagcagattg atcagcgcaa tcgtctgcgt cagctcgatc aggctcttgc cgtcctcctc
    10801 g
//
LOCUS       DAORPM010000059        10604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1420_length_10604_cov_11.812778, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000059 DAORPM010000000
VERSION     DAORPM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_10604_cov_11.812778"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="PLD53_04435"
     CDS             <1..177
                     /locus_tag="PLD53_04435"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HRC60187.1"
                     /translation="KWVTERAYDNPKFVEDLVRDIALRLMEEPRIAEWKVASENFESI
                     HNHSAYAELFGVNP"
     gene            202..1533
                     /locus_tag="PLD53_04440"
     CDS             202..1533
                     /locus_tag="PLD53_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC60188.1"
                     /translation="MHKQRIAVVGSGISGLASAWLLSREHQVTLFEAGARLGGHTNTV
                     DVTLEGKTHPVDTGFLVFNERTYPNLIAMFEHLGVASVETEMSFAVSLENPDLEWAGS
                     DLFTVFGQKRNLLRPSFWSMLSDILRFNRESTAWLATHPDNARSLQDFLDEGGYSSAF
                     ADWYLLPMAAAIWSCPTGQMREMPLATFIRFCRNHGLLQIFDRPMWRTVKGGGREYVQ
                     KIAARLDDIRLACPVTAVTRQAEGLQVVHARGTEQFDQVVLACHSDQSLGILGTTATD
                     AQREVLSAIRYQPNRAVLHTDPALLPRERKLWSAWNYFSGPADKSGAPGVDANEQPVG
                     VSYLINTLQPLPFKTPVVVTLNPPREPDPAKVIASFEYAHPIFDGPAIAAQHKLAQIQ
                     GKNGIWLAGAWGSYGFHEDGLKSALRVVNGLGVQAPWQVSVAERQRATESA"
     gene            1541..2317
                     /locus_tag="PLD53_04445"
     CDS             1541..2317
                     /locus_tag="PLD53_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRC60189.1"
                     /translation="MNDRPQLFLGHVMHRRLRPVVNSFVYPVFFVRLPVRDLASANCG
                     IFGVDRANILSFRQGDHGARDGSPLLPWIEALLKDRGLSADGEIVLQTFPRVFGIVFN
                     PVSFWYCHERGGALIAILAEVNNTFGGTHSYLLHGKGKPLVDGEELVAEKVFHVSPFN
                     EIEGGYRFRFHLRRSVPLARIDYDDADGELLLTSISGKARGWGTAALAGALLRMPLLT
                     AGVIFRIHWQALKLWLKGVPFRGVRVPPAPISPPLRESTK"
     gene            2314..3513
                     /locus_tag="PLD53_04450"
     CDS             2314..3513
                     /locus_tag="PLD53_04450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRC60190.1"
                     /translation="MNTLNTSMIFRGSESLARDTRLVCGLLEKLRGGLLEMRLPNGSS
                     TLFGDGEHGVVMHVRDESVFSMVLARGDIGLAESYLDGHWDSPDITGLLTLLARNRDV
                     LKRAVYGSWHELLAARVRHWLNRNSRIGSKRNIMAHYDLGNDFYRLWLDPSMSYSAAI
                     YRAGDDGSLEAAQHAKYRRILSRLKARPGQHVLEIGCGWGGFAEMAVAAGLQVTGITL
                     SPAQLAWGQKRVPAADLRLQDYRDNHETYDHVVSIEMFEAVGERFWPGYFKSVARALK
                     PGGKAVIQSITIRDDLFASYRKGTDFIQQHVFPGGMLPSRQAFRAAAARQGLVVHGEY
                     AFGIDYARTLAEWRHAFEAKWPQISALGFDERFRRLWRMYLCYCEAGFLAGNIDVVQF
                     ELSHRHG"
     gene            3476..4093
                     /locus_tag="PLD53_04455"
     CDS             3476..4093
                     /locus_tag="PLD53_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HRC60191.1"
                     /translation="MSSSSSFRTVMAERPIQLDGIQRQPGTLVRKGPSSGLRRRLLLA
                     LIAAPLSAFAVGETAGQRGELRRWGSGEFRRFGFLVYEATLWAADDPVRPPLALKLTY
                     RRNIDGELIAEASIKEIRKLGMADEATLNRWGELMTGLFPDVKPGDTIVGHYLPEGAR
                     FTFNEQPLGSIDDPAFARAFFAIWLDPNTSEPDLRAALLTRPTAG"
     gene            4101..5324
                     /locus_tag="PLD53_04460"
     CDS             4101..5324
                     /locus_tag="PLD53_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC60192.1"
                     /translation="MTGNPVFAYGLLGLPLAFAALPIYVHVPRYYAEAADLPLAMLGA
                     ILLGTRLLDAGIDPWLGWLADRVSRRRMLALSLAPFALGFVALLNPPVEHAGLWLLAS
                     LTLTYLGFSAATVAYQAWGADIGTDSILRTRLTAAREGFGLLGVVLAAALPTVLATDL
                     DRGIERLSWILPPLVLIAAATTFSRVAAGPVVAVTRVPLLPSLRKVFADIAFRRLLLI
                     FVANGIAAALPATLFLFFVADVLQLEKVSGLLLALYFVAGAASLPLWVRLSARYGRVL
                     AWLGAIGLSIISFAGASQLGSGDLWPFAAICIASGLALGADLALPVAIAADLGERQGQ
                     AGAYFGVWNLVAKLNLALAAGLSLPLLGLLGYVPGSGNGLWSLTLAYALLPLAFKALA
                     GCLLWHWRHTLEIES"
     gene            5321..5842
                     /locus_tag="PLD53_04465"
     CDS             5321..5842
                     /locus_tag="PLD53_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HRC60193.1"
                     /translation="MKLMLAAAFSLGLAGCAATGVEHYRAERPVLNLKTYLNGTLDGW
                     GMFQGRSGEVKKRFHVVIDAKWDGETGVLDENFTWSDGTTSRRVWTLTRQRDGSFRGT
                     ADDVVGEAIGEVAGNALRWRYVLALPVDGKIYNVDFDDWMFLMDDKVMMNRSWMSKWG
                     FNLGEVTLTFVKR"
     gene            5839..6594
                     /locus_tag="PLD53_04470"
     CDS             5839..6594
                     /locus_tag="PLD53_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC60194.1"
                     /translation="MNPKITDWNDKRIWLVGASTGIGAAVARELAGRGARLALSSRKA
                     DKLAALGIANALLLPCDATDTTSLAAARRNLLEAWGAVDLVVYLAGDYVPMRADQFDL
                     AIAERVVAVNFTGAMRFAAAVLPDLRPGGGIAFVASVAGYRGLPKALCYGPGKAALIH
                     FAEVLHLDLAPKGIGVWVINPGFVATPLTAQNDFSMPALLTPEQAAKAMVDGFGSANF
                     EIHFPKRFTRIMKLLAHLPYRWYFPLIRRLTGG"
     gene            6596..7018
                     /locus_tag="PLD53_04475"
     CDS             6596..7018
                     /locus_tag="PLD53_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC60195.1"
                     /translation="MNLDPLIRFYDEFTPESVARFGEFYSADAYFKDPFNEVRGIDAI
                     QRIFGHMFSQVTEPRFVVTEKVVDDNGALLVWEFCFRMKLWGKGESHVMHGASHLKFD
                     AEGKVCYHRDYWDTAEELYMKLPIMGSVLRCFRKALSA"
     gene            complement(7049..8224)
                     /locus_tag="PLD53_04480"
     CDS             complement(7049..8224)
                     /locus_tag="PLD53_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRC60196.1"
                     /translation="MNLLDISFLADLAAIRHDIHAHPELAFNEARTAELVARELTAYG
                     LEVHRGLAKTGVVGVLRKGSSTRAIGLRADLDALPLLEKNSFSHRSRHEGRMHACGHD
                     GHTTMLLGAARYLAENRDSLDFDGTVYFIFQPAEESEGGAAVMIEDGLFKMFPMDAVF
                     GLHNWPGIPVGEMAVRPGAVMAGTSAFEISVSGHGCHAAMPNQGVDTLVVASQLVLAL
                     QTVVARNLHPCESAVVSVTQIHGGEALNIIPDDAVLRGTIRSFKPEVQELVEKAIERL
                     CSGIASAFGAQIKAVFDHRYPPTVNTVPEAALCCRVAEALLGTDKVRKNELPSMGAED
                     FAYMLQEKPGCYVWLGNGPGAGGCTLHNPHYDFNNELIPYGISYWVRLVETALKREA"
     gene            8307..9167
                     /gene="rapZ"
                     /locus_tag="PLD53_04485"
     CDS             8307..9167
                     /gene="rapZ"
                     /locus_tag="PLD53_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRC60197.1"
                     /translation="MQLIIISGLSGSGKSIALNMLEDADYYCVDNMPSQLLQQLVDQL
                     AQQGYDKVAVAIDIRGGKSIAMLPQQLASLRANELDLQFLFLDAKTETLLKRYSETRR
                     RHPLADEQRTVTEAIAEERDRLEGLSELGHHIDTSDLKPSALREWVRQFISTETRRGL
                     TLIFESFGFKHGVPLDAELVFDVRCLPNPHYEQALRPLTGRDTPVITFLEAEPDVLNM
                     RDDIARFIDTWLPCYIRDNRNYLTVGIGCTGGQHRSVYFAECLGRKFRDQARVLVRHR
                     ELPSIPTGNP"
     gene            9167..9541
                     /locus_tag="PLD53_04490"
     CDS             9167..9541
                     /locus_tag="PLD53_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HRC60198.1"
                     /translation="MHWMALFKPGDWLVISFGVIVCAVSWPLAWQSGAADKAVVKRGG
                     EVFAELDLSRNRSIEVPGPLGITKISVDKRRVRVVSDPGPRQYCVRQGWLARPGEIAI
                     CAPNQVSVQVQGGKDAYDSLSY"
     gene            9522..10061
                     /locus_tag="PLD53_04495"
     CDS             9522..10061
                     /locus_tag="PLD53_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HRC60199.1"
                     /translation="MTRSVIRLVTSPEDHRIAQLAAAAIGLALIDAAIPLPLPGVKPG
                     LANIVTLIVLARFGWATAAWVSGLRVVAGSLLLGQFMSPGFFMSLTGSLFSLIVLAFA
                     VRLPGRWFGPVSWSVLAAFAHIGGQLLLARLWLIPHNGLFYLVPLFSAASLAFGIING
                     LIAARLLEELPDTQPDAPR"
     gene            10066..>10604
                     /locus_tag="PLD53_04500"
     CDS             10066..>10604
                     /locus_tag="PLD53_04500"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HRC60200.1"
                     /translation="MPKTICLALTGASGMPYGIRLLECLLEAGCHVQLLYSQVAQVVA
                     NQEMAIELPSRATDAQTYFRERYAAHPGKLEVYGREEWFAPVASGSNPPDAMVICPCT
                     MGTLASIAQGLAKQLIERAADVVLKEGRKLILVPRETPFSIIHLENMLRLARAGAVIL
                     PPNPGFYHHPQGVEDLVDFV"
BASE COUNT         1833 a         3308 c         3228 g         2235 t
ORIGIN      
        1 aaatgggtca ccgagcgcgc ctacgacaac ccgaagttcg tcgaggacct ggtgcgcgac
       61 atcgcgttgc gccttatgga ggagccgcgc atcgctgaat ggaaggtcgc ttccgagaat
      121 ttcgagtcga ttcacaacca ttcggcctat gccgagctgt tcggtgtcaa tccgtaaacc
      181 ggccaacgag gggagataac catgcacaaa caacgaatcg cggtcgtcgg ctcaggcatt
      241 tcaggtctgg ccagcgcctg gctgttgagc cgagagcatc aagtgacgct cttcgaagcc
      301 ggcgcccggc tcggcggcca taccaacact gtcgacgtca cgctggaagg caagacgcat
      361 ccggtggata ccggcttcct ggtcttcaat gaaaggacct atcccaacct gatcgcgatg
      421 ttcgagcatt tgggcgtcgc cagcgtcgag accgagatgt cctttgccgt aagtctcgag
      481 aacccggacc tcgaatgggc cggcagcgat ttgttcacgg tattcggcca gaagcgcaac
      541 ctgctgcgcc cgagtttctg gtccatgctt tccgatatcc tgcgtttcaa tcgcgagagt
      601 acggcctggc tggcgacgca tcccgacaac gcgcgcagcc ttcaggattt cctcgatgag
      661 ggtggttatt cgagcgcttt tgccgactgg tatctgctgc cgatggccgc cgcgatctgg
      721 tcctgcccga ccggccagat gcgcgagatg cccctcgcca ccttcatccg cttttgccgg
      781 aaccacggtc tgctgcaaat ctttgaccgc ccgatgtggc gcacggtgaa gggcggcggg
      841 cgcgagtacg tgcagaagat tgccgcccgc ctcgacgata tccgcctggc gtgcccggtg
      901 acggcagtga cccgtcaagc ggaagggctg caggtcgtcc atgcccgcgg caccgaacaa
      961 ttcgaccagg tcgtgctggc ctgccacagc gaccaatcgc tgggcattct gggcacgacg
     1021 gcgaccgacg cgcagcgcga ggtgctctcc gcgatccgtt accagccgaa ccgtgccgtt
     1081 cttcataccg atccggccct gctgccccgc gagaggaagc tgtggtcggc atggaattac
     1141 ttttccgggc ctgcggacaa atccggcgca cccggagtgg atgccaatga gcagccggtc
     1201 ggcgtttcct atctgatcaa tacgctgcaa cctttgccct tcaagacgcc ggtggtggtg
     1261 acgctgaatc cgccacgcga gcccgacccg gccaaggtca tcgcctcatt cgagtatgcc
     1321 cacccgattt tcgacggccc ggccatcgct gcacagcaca agcttgcgca gattcaaggc
     1381 aagaacggca tctggctggc cggcgcctgg ggcagctacg gcttccacga ggacggcctg
     1441 aagtcggcgc tgcgcgtggt caacggcctc ggtgttcagg caccctggca agtcagcgtc
     1501 gccgaaagac agcgcgcaac ggaaagcgcc tgaggtggcc atgaacgacc gtccgcaatt
     1561 gttcctcggc catgtcatgc accggcgcct gcggccggtg gtcaattctt tcgtctatcc
     1621 ggtgttcttc gtccggctgc cggtgcgtga tctggcatcc gcgaattgcg gcattttcgg
     1681 cgtcgaccgc gccaacatcc tgagcttccg gcaaggcgat catggcgctc gcgacggcag
     1741 cccattgttg ccgtggatcg aggcgctgct caaggaccgt gggctatctg ccgatggcga
     1801 aattgtcctg cagaccttcc cgcgtgtttt tgggatcgtc ttcaatccgg tgagcttctg
     1861 gtattgccat gagcgtggcg gggcactgat cgccatcctg gccgaggtca acaacacttt
     1921 cggcggcacc cacagctacc tgctgcacgg caagggcaag ccactggtcg acggcgaaga
     1981 gttggtcgcc gaaaaagttt tccatgtttc gcccttcaac gaaatcgaag gcggctatcg
     2041 cttccgcttc catctccggc gcagcgttcc tttggcgcga atcgactacg acgatgccga
     2101 cggcgaactg ctgcttacct cgatatcagg taaggcgcgg ggctggggga cggcagccct
     2161 ggccggagcc ctgttgcgca tgccgttgct gaccgccggc gtgatcttcc gcatccactg
     2221 gcaagccctg aagctttggc tgaaaggggt gccgtttcgc ggcgtccgcg ttcctcctgc
     2281 acctatctct ccacctctcc gggaatcgac aaaatgaata ctttgaacac ctcgatgatc
     2341 ttccgcggca gtgaaagtct ggcccgcgac acccgtctgg tctgtggtct gctcgaaaag
     2401 cttcgcggcg gcctgctcga aatgcgcctg cccaacggtt ccagcaccct gttcggcgat
     2461 ggcgagcatg gggtggtgat gcacgtccgc gatgaatccg tgttcagcat ggtgcttgcc
     2521 cgcggcgaca tcggcctggc cgagtcctac ctcgatggtc actgggattc gcccgatatc
     2581 accggactgc tgaccctgct ggcgcgcaac cgtgacgtgc taaagcgggc cgtgtacggt
     2641 tcgtggcacg agctgctggc ggcacgtgtg cgccactggc tcaatcgcaa cagccgaatc
     2701 gggagcaagc gcaacatcat ggcccactac gatctgggca atgatttcta ccggctgtgg
     2761 ctggatccga gcatgagcta ctcggccgcg atctaccgcg cgggcgacga tggctccctg
     2821 gaagccgccc agcatgccaa gtacaggcgc attctcagtc gcctgaaggc ccggcccggc
     2881 cagcatgtgc tggaaattgg ctgcggctgg ggcggcttcg ccgaaatggc ggtagccgcc
     2941 ggcctgcagg tgaccgggat aacgctgtca ccggcccagc tcgcctgggg acagaagcga
     3001 gtgccggcgg ccgatctgcg cctgcaggat tatcgcgaca accacgagac ctacgatcac
     3061 gtcgtttcca ttgaaatgtt cgaggcggtt ggcgagcgct tctggccggg ctatttcaag
     3121 tctgtggcca gagcgctgaa gccgggcggc aaggcggtca tccagagcat taccattcgc
     3181 gacgacctgt tcgccagtta ccgcaagggg accgacttca tccagcaaca cgttttcccc
     3241 ggcggcatgc tgccgtcgcg ccaggctttc cgcgccgccg cggcccgcca gggcctggtc
     3301 gtccacggcg aatacgcttt cgggatcgac tacgcccgga cgctggccga gtggcgccat
     3361 gcttttgagg ccaagtggcc gcaaatttcc gcactcggtt ttgacgagcg ctttcgccgc
     3421 ctgtggcgca tgtacctgtg ctattgcgag gccggctttc tggccgggaa tatcgatgtc
     3481 gtccagttcg agctttcgca ccgtcatggc tgagcggcca attcagttgg acggtatcca
     3541 gcgtcaaccg ggaacattgg tccgaaaagg cccttcgtcc ggtcttcgcc ggcgcctgct
     3601 gctggcgctg atagccgcgc cgctgtcggc gtttgctgtt ggcgaaacag ctgggcagcg
     3661 gggcgagctc aggcgttggg gcagtggcga atttcgccgc ttcggctttc tcgtttatga
     3721 agcgacgctc tgggcggccg acgaccccgt tcgcccgcct ctggccctga aactcaccta
     3781 tcgccgcaat atcgacggcg agcttatcgc cgaggccagc atcaaggaaa tccgcaagct
     3841 ggggatggcc gacgaggcga cgctgaatcg ctggggcgag ctgatgacgg ggttgtttcc
     3901 cgatgtcaaa ccgggtgaca ctatcgtcgg gcactacctg ccggagggtg cccgctttac
     3961 cttcaacgag caaccgcttg gcagcatcga cgatcccgcc ttcgcgcgcg cctttttcgc
     4021 catctggctt gatccgaata ccagcgaacc ggatttgcgt gccgcgctac tgacgcggcc
     4081 aaccgcgggt tgagaacgcg atgaccggca atcctgtttt cgcctacggc ctgcttggcc
     4141 tgccgctggc ctttgcggct ctgccgatct atgtgcatgt gccgcgctac tacgctgaag
     4201 ctgcggatct gccgctggcg atgctcggcg cgatcctgct cggcacccga ttgctcgatg
     4261 ccggcatcga cccctggctg ggctggctgg ccgaccgcgt ctcgcggcgg cggatgctgg
     4321 cactgtccct ggcgcccttc gcccttggct tcgtcgcgct gctcaatccg ccggtcgagc
     4381 acgccggttt gtggctgctc gcttcgctga cgctgacgta tctcggattt tccgccgcca
     4441 ccgtcgccta ccaggcctgg ggtgccgata tcggcaccga ttccattctg cgcacccggc
     4501 taacggctgc ccgtgagggt ttcggtctgc tcggcgtggt cctcgccgca gcgctgccga
     4561 cggtgctggc caccgatctg gaccggggca tcgagcgcct gagctggatc ctgccaccct
     4621 tggtgttgat cgctgccgcc accacctttt cccgtgtcgc tgccggcccg gtcgtggcgg
     4681 tcacccgggt tccgctgctt cccagcctgc gcaaggtatt cgccgacatc gctttccgcc
     4741 gtctgctgct gattttcgtc gccaacggca tcgccgccgc cctgccggca acattgttcc
     4801 tcttttttgt ggccgatgtc ctgcaactgg agaaggtcag tgggttgctg ctggcgctgt
     4861 atttcgttgc cggcgcggcg tcactgccgc tgtgggtgcg gctctcggcc cgttatggac
     4921 gggtgctggc ctggctgggc gccataggcc tgtcgatcat ttcttttgcc ggcgccagcc
     4981 agcttggcag cggtgacctg tggcctttcg cggcgatctg catcgcctcc gggctggcac
     5041 tgggcgccga tctggccctg ccggtggcca tcgccgccga cctcggcgag cgccaggggc
     5101 aggccggcgc ctatttcggc gtctggaacc tggtcgccaa actcaacctg gccctggcgg
     5161 ccggactttc attgccgctg ctcggcctgc ttggttacgt accgggcagc ggcaacggcc
     5221 tgtggtcgct gaccctggcc tatgccttgt tgccgcttgc cttcaaggcg ctcgccgggt
     5281 gcctgttgtg gcactggcgt catactctgg agatcgaatc atgaaactca tgcttgctgc
     5341 tgcattctca ctcggcctgg ccggctgtgc cgccaccggt gtcgagcact atcgtgccga
     5401 acggccggtg ctcaatctga agacctacct gaatggcacg cttgacggct gggggatgtt
     5461 tcagggacgt tccggtgaag tcaagaagcg ctttcacgtc gtcatcgacg ccaaatggga
     5521 tggcgaaaca ggcgttctgg acgaaaattt cacatggtct gacggaacca catcgcgccg
     5581 tgtgtggacc ctgacccgac agcgggatgg aagttttcgg gggaccgccg acgacgtggt
     5641 tggggaagcg attggcgaag tcgccggcaa tgccttgcgc tggcgctacg tgctggcact
     5701 gccggttgac ggcaagatat acaacgtcga tttcgacgac tggatgttcc tgatggacga
     5761 caaggtgatg atgaaccgct cctggatgtc gaaatggggc ttcaacctcg gcgaagtcac
     5821 gctgactttt gtcaaaagat gaacccgaag attaccgact ggaacgacaa gcgcatctgg
     5881 ctggttggtg cctcgaccgg tatcggcgcc gcggtcgcgc gcgaactggc cggtcgcgga
     5941 gccaggttgg ccttgtcgtc ccgcaaggcc gacaagctcg cggcactggg tatcgccaat
     6001 gccctgctgc tgccctgcga tgcgaccgac acgaccagtc tggctgcggc ccgcaggaac
     6061 ctgcttgaag cctggggcgc tgtcgatctt gtcgtctatc tggccggcga ctacgtgccg
     6121 atgcgcgccg atcaattcga cctggccatt gccgagcgcg ttgtcgctgt caatttcacc
     6181 ggtgccatgc gcttcgcggc ggccgtcctg cccgacctcc ggccgggcgg cggcatcgcc
     6241 ttcgtggcca gcgttgccgg ctatcgcggg ctgcccaagg ctttgtgcta cgggccgggc
     6301 aaggcggcac tgatccactt cgccgaggta ctgcatctgg acctggcgcc gaaaggcatc
     6361 ggcgtctggg tgatcaaccc aggtttcgtc gcaacaccgc tcacagcgca gaatgatttc
     6421 agtatgccgg cgctgctgac tccggaacag gcggcaaagg ccatggtcga cggctttgga
     6481 tcggccaatt tcgagattca ctttcccaaa cgctttaccc gcatcatgaa gctgctggcg
     6541 catttgcctt accgctggta tttccccctg atccgtcgcc ttaccggagg ctgagatgaa
     6601 cctcgaccca ctgatccgct tttacgacga attcactccc gagagcgtcg cccgcttcgg
     6661 cgagttctac agcgccgacg cctacttcaa agaccctttc aacgaagttc gcggcattga
     6721 cgccattcag cgcatttttg gtcacatgtt cagccaggta accgaaccgc gtttcgtcgt
     6781 taccgaaaag gtcgtggacg acaatggcgc cctgctggtc tgggagttct gtttccggat
     6841 gaaattgtgg ggcaaaggtg agtcccacgt gatgcatggc gcttcacacc tgaagttcga
     6901 tgccgagggc aaggtctgtt atcaccggga ttactgggat accgccgaag agctctacat
     6961 gaagctgccg ataatgggta gcgtcctgcg ctgtttcaga aaagctctgt cagcgtgatt
     7021 ttccagcggc aagatgctgc ctgctaatct aggcttcgcg cttcagcgcg gtttccacca
     7081 gccttaccca atagctgatg ccgtacggaa tgagttcgtt gttgaaatcg tagtgcgggt
     7141 tgtgcaaggt acaacccccc gcgccggggc cattgcccaa ccagacgtag caaccgggtt
     7201 tctcctgcag catgtaggcg aagtcctccg cgcccattga gggcaattca ttcttgcgca
     7261 ccttgtccgt accgagcagc gcttcagcca cccggcagca gagcgcggct tctggaacgg
     7321 tgttcaccgt cggcggatag cggtggtcga atacggcctt gatctgtgcg ccgaaagcac
     7381 tggcgatgcc gctgcacagc cgttcgattg ccttctcgac cagctcctga acttcgggct
     7441 tgaagctgcg gatcgtgccg cgcagaaccg catcgtccgg gatgatgttc agcgcttctc
     7501 caccgtgaat ctgcgtcacg ctgacgaccg ccgattcgca ggggtgcaga ttgcgagcaa
     7561 cgacggtctg caaagcgagc actaactgac tggcaacgac cagtgtatca acgccctgat
     7621 tcggcatcgc cgcatggcaa ccgtgaccgc tgactgaaat ctcgaacgcg ctggtgccgg
     7681 ccatcaccgc tccgggcctg acggccattt cgccaaccgg aatgcccggc cagttgtgca
     7741 gaccgaagac ggcatccatc ggaaacatct tgaacaatcc gtcttcgatc atcaccgccg
     7801 cgccgccttc cgattcttcg gccggctgga atatgaaata gacggtgcca tcaaaatcca
     7861 ggctgtcccg gttttcagcc aggtaacgcg ccgcgccaag cagcatggtg gtgtgaccgt
     7921 catgaccgca ggcatgcatc cggccttcat gccgggaacg atgcgagaag gaattctttt
     7981 caagcaaggg cagggcatcg aggtcggcac gcagaccgat ggcgcgcgtc gaactgccct
     8041 tgcgcaaaac gccgacgacc ccggttttgg ccagcccgcg atgtacctca agaccgtagg
     8101 cggtgagttc gcgcgcaacc agttctgcgg tgcgcgcttc gttgaaggcc agctcgggat
     8161 gcgcgtgaat atcgtgacgg atggcggcga gatcggccag aaagctgatg tccagaaggt
     8221 tcatggctgc tgatccggaa atagggaaga ccgttcacgt tagccccgct tgcccgtgta
     8281 agcaaggttc cgctatgctg acgttcatgc aactcattat catcagcggc ctctcgggtt
     8341 ccggcaagtc aatcgctctc aacatgcttg aggatgcgga ttactattgc gtcgataaca
     8401 tgccttcgca attgttgcag cagcttgtgg accagcttgc ccagcagggc tacgacaagg
     8461 tcgctgtcgc catcgacatt cgcggtggca agagtattgc gatgctgcca cagcagcttg
     8521 cttcgcttcg cgccaacgag ctggatttgc agtttctttt cctcgacgcc aaaaccgaaa
     8581 ccctgctcaa acgctattcc gagacgcgcc gtcgacatcc gttggccgac gaacagcgca
     8641 ccgtgaccga agccattgcc gaagagcggg atcgccttga aggcctgtcc gaactgggcc
     8701 atcacatcga tacgagcgat ctgaaaccaa gcgcactgcg tgaatgggtt cgtcagttca
     8761 tctccaccga aacgcgacgg ggcttgacgc tgatctttga atcctttggt ttcaaacacg
     8821 gcgttccgct cgacgctgaa ctggtctttg acgttcgctg cctgccgaac ccgcactacg
     8881 agcaggccct gcgtccgctc accggtcgcg ataccccggt gattaccttt cttgaggccg
     8941 aacccgatgt gttgaacatg cgcgacgaca tcgcgcgctt catcgacacc tggctgcctt
     9001 gctacattcg tgacaatcgc aattacctga ctgtgggcat cggctgtacg ggcggccagc
     9061 atcgttccgt ttactttgcc gagtgccttg ggcgcaagtt ccgcgatcag gcgcgcgtcc
     9121 tggtccgtca ccgcgaactg ccttcgattc cgaccggcaa cccctgatgc attggatggc
     9181 gctgttcaag ccgggtgact ggctggtgat ttcgttcggg gttattgtct gcgctgtttc
     9241 ctggccgctg gcatggcaaa gcggtgctgc cgacaaagcc gtcgtcaaac gtggtggcga
     9301 agtcttcgcc gagcttgatc tgtcacgcaa tcgcagtatc gaggttccgg gtccgttggg
     9361 cattacgaaa atctctgtcg acaagcgccg cgtgcgcgtt gtctccgacc cggggccccg
     9421 tcaatattgt gtacgccagg gctggctggc gcgtcctggt gaaatcgcca tctgcgcgcc
     9481 gaatcaggtc agcgtacagg tgcaaggtgg caaggacgcg tatgactcgc tcagttatta
     9541 ggctggtcac ttcgccggaa gaccaccgca tcgcgcagct tgcggcggcg gcgattggtc
     9601 tggcgctgat cgatgcggcg attcctttgc ccctgcccgg tgtaaagccg ggactggcca
     9661 acatcgtgac actgatcgta ctggcacgtt tcggttgggc aacggccgcc tgggtcagcg
     9721 gattgcgagt tgtcgcggga agtctgctgt tggggcaatt catgtctccg gggttcttca
     9781 tgagcctgac agggtctttg ttcagcctga tcgtactggc ttttgctgtt cgcctgccgg
     9841 ggcgttggtt cgggccggtc agctggagcg ttcttgccgc cttcgcacat atcggggggc
     9901 aactgcttct cgcccggctg tggctgattc cgcataatgg tttgttctat ctggtgccgc
     9961 ttttttccgc ggcctcgctg gctttcggta tcatcaacgg actgatagcc gcacgcctgc
    10021 tagaagaact tccggacacc caacccgacg ccccgaggtg aagtaatgcc caagacgatc
    10081 tgccttgccc ttaccggagc ttccggcatg ccttacggca tccggctgct tgaatgtctg
    10141 ctcgaagcgg gttgccacgt acaactgctc tattcgcaag tcgcccaagt cgtcgccaat
    10201 caggaaatgg caattgaact gccgtcacga gctacggatg cacagaccta ttttcgcgag
    10261 cgctatgccg cacaccccgg caagctggag gtttatggcc gtgaggagtg gtttgcgcct
    10321 gtcgccagcg gatcgaaccc accggacgcc atggtcatct gcccatgcac catgggcacg
    10381 ctggcgtcga ttgctcaggg gctggccaag cagctgatcg aacgtgctgc ggatgtggtg
    10441 ctcaaggaag ggcgcaaact gatactggtg cctcgcgaaa cacctttctc gataattcat
    10501 ctggaaaaca tgctgcgtct cgcgcgcgcc ggcgccgtca tcctgcctcc caaccctggt
    10561 ttttaccacc atccgcaggg cgttgaagat ctggtcgatt tcgt
//
LOCUS       DAORPM010000060        10514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1435_length_10514_cov_12.644421, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000060 DAORPM010000000
VERSION     DAORPM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10514
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1435_length_10514_cov_12.644421"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..539
                     /locus_tag="PLD53_04505"
     CDS             282..539
                     /locus_tag="PLD53_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRC60201.1"
                     /translation="MPRNTSVTLGEHFEGFIAQQIDAGRFESKSEVVRAAMRLLEEHE
                     QKVSALRQALVEGETSGEPVPFTMASIIEEARQECYKGAQG"
     gene            540..1004
                     /locus_tag="PLD53_04510"
     CDS             540..1004
                     /locus_tag="PLD53_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60202.1"
                     /translation="MPIIGHLAEAGIVIFDAFREGNIAPATQNFEFIQACEARLPKGH
                     RMAHVRLDSAGYQADIVNYCENTGKTFAIGGRLDAPTQQAIAGIPESDWKRYADCAVA
                     ETLHSMNETRKAFRLIVVKYKRQAELFDDAPRYHVIVSNCVESTTDTLIWYR"
     gene            1029..1334
                     /locus_tag="PLD53_04515"
     CDS             1029..1334
                     /locus_tag="PLD53_04515"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC60203.1"
                     /translation="MKELKIGFGMGRMPCGQLIVNAAFFRIGVIAHNLFVLFKHSTLG
                     ADWQRYKVATIRWRLFHLPGKVVRHAGAWVLKVAVDTVDRFRTIRTRSFAQAVANSP"
     gene            1612..1881
                     /locus_tag="PLD53_04520"
     CDS             1612..1881
                     /locus_tag="PLD53_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HRC60204.1"
                     /translation="MSTHTSHPEIIKRLKRAEGHLRSTITMLEDSRDCLAIAQQLQAV
                     ESAVGNAKKTLVHDHIDHCLEQAVRDAPQSAEETIREFKAITKYL"
     gene            1892..3010
                     /locus_tag="PLD53_04525"
     CDS             1892..3010
                     /locus_tag="PLD53_04525"
                     /inference="COORDINATES: protein motif:HMM:NF007454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HRC60205.1"
                     /translation="MTSFSTLLAQGAGNAWLFIPSAILLGGLHGLEPGHSKTMMAAFI
                     VAIRGTVGQAVLLGVSATISHTAVVWVIALGGMYFGQKWDAETSEPYLQVASALVIIG
                     VALWMIWRNWRQQQLAEQDHDHDHHHDDSKRIDTGHGVVRLDVFEDGVPPRFRLFAES
                     GHGHTWTADQVRIETERPDGSRQSFRFVQRDGFVESEQEIPEPHEFVARLRLGHEHHS
                     HDYDVEFVEHDHQHHAIKDYEGLDVSAPGYQDPHELAHANDIRHRFTNREVTTGQIVM
                     FGLTGGLIPCPASVTVLLLCLQLKEIALGATLVLSFSIGLALTMVLSGALAALSVKHV
                     SRRWSGFGKFARRAPYFSAALILLVGLYVGYQGLKALA"
     gene            3979..4974
                     /locus_tag="PLD53_04530"
     CDS             3979..4974
                     /locus_tag="PLD53_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC60206.1"
                     /translation="MGIQTTPLQNDTDLVKASYPAHVVVPPEAEQVISSPVAGLVAQL
                     LVQQNQVVPSGAPLVRIVSPELGQLQLQLLQATVRATLARQAAQREHALFDEGIIAKR
                     RVQEARAALQEAEATLNQAKAALRLSGMPSATIERIATSGKPQDSVTLLATQAGIVTV
                     IAVKPGQRVEAATALLHVAQTDSLWLDIQLPVSERVGWPAGTQVKVLGRSLTARILSV
                     SPTVSHGSQTVVLRAAVEGKTGEVRPGELVTVELPITATQGNWDVPLSAVVHDGEQAY
                     LLVRTADGFEARPVKVVVSAGQRVRVQGPLKEGEQIAISGVVALKGAWLDAKESK"
     gene            4974..8057
                     /locus_tag="PLD53_04535"
     CDS             4974..8057
                     /locus_tag="PLD53_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRC60207.1"
                     /translation="MLARLVQLSLSQRLFVLLATLIVAGAGWMAYLGLPIDAFPDVSS
                     TQVKIIMKAPGMTPEEVESRIAVPIEVEMLGIPKTKILRSVTKYGLVDVTIDFEDGTD
                     IYWARQQVSERLGGLTDSLPAGITGGMAPITTPLGEMFMFTVDADGLSLAERRSLLDW
                     VIRPALRTVPGVADVNALGGVVRAFEVVPDQIKMAASGVTVAQLKEAIDANNRNDGAG
                     RLGEGDEVLLVRSEGSVTSLDDLRAIVVTIKDDSPVRLGNLAEVKIGTVTRYGVVTQD
                     GKAEAVQGLVLGLAGANAQKVVEGVRKKLDELQPTLPKDVQLGVFYDRASLVSKAVGA
                     VSAALLEATVLVIILLGLFLGNIRAAVTVALVLPLAALITFILMRGFGMSANLMSLGG
                     LAIAIGMLLDAAVVVVENIVQRLATDPTAGKLPRLHTIYRAVREVAVPVSSGILIIIT
                     VFLPLLTLQDLEGKFFVPVALTIVFALAGSLLLSLTVIPVLASYLLREVKHEDPWLPR
                     KLLALYEPALAWGLRRQGIVVGVAVLLLLGAGGVYTQVGKTFMPTMDEGDLIVGIEKL
                     PSISLEQSAALDLKIHQALMKAIPEVTGIVARAGSDEIGLDPMGLNQTDTYLVLKPRD
                     DWRMKSKEALMDEIRKVLDPMPGISYSFTQPIEMRVSEMIIGVRGDLAVKIFGPDLDQ
                     LNTYASEVEALLKTVPGNQDVYTVQNDGVQYLQVVVDRLQAGSLGLSVEEIQDALRTQ
                     IEGQRAGIVIEGNRRTPIVLRGTEAVRMSPAEFGAMRITTQGGSTVPLTSVAKLQRAA
                     GPVKIDREMGSRYSVVIANVGGRDLVGFVEEARALVTEKLSLPTGYRITWGGQFENQQ
                     RAAARLAVVVPVALGLVFVLLFSTFRSVRQALLILSNVPFALVGGIVALWVTGEYLSV
                     PASVGFIALLGIAVLNGVVLVSYFNQLHSEGMKLSDVVVQGAKRRLRPVLMTASITAF
                     GLIPLLFASGPGSEIQRPLAIVVIGGLTTATVLTLMLLPILYLRFAFPRREVPHV"
     gene            8050..8352
                     /locus_tag="PLD53_04540"
     CDS             8050..8352
                     /locus_tag="PLD53_04540"
                     /inference="COORDINATES: protein motif:HMM:NF023014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HRC60208.1"
                     /translation="MSELCLTLVCPPEVEEKLLDLLLLWPGATIFTSTATAAHGLAHE
                     SLDQTEQVLGRARATEVQVICAAAGQAALLAALRQQFSGVGLRYWVTPVVEAGEIA"
     gene            8349..9587
                     /locus_tag="PLD53_04545"
     CDS             8349..9587
                     /locus_tag="PLD53_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRC60209.1"
                     /translation="MMVRLLLIGIFAVVKAASAAQPMNPPGLMPTAIARPLFEQDPGV
                     AAARAGLDVALQEASILDQSPYEWTARVDGQQRRVENGPRYNEWFVGVEKTIRLPGKA
                     TADRSIGKATVDESRARYGEALLTSARELMELWVDWLAAERARELGGSSLQSMQASLA
                     AVEKRARAGDASKLDLGITRAELAEQRRMDNDAKTQAAAAWLRLSTRFPGVERQLLAL
                     PEPLVLGSEAPSWQERILAETHALKLVQAQVRKAQAQAERARAEKIPDPTFGVYTASE
                     IGGRERIPGVTISIPIPGGARDLRSAQAIAAVEVSRQEAELKQRQLQAGIASAVATAR
                     GGYESFQIANEGAAAMQENARLMQRAYALGEAELQAFLLTRRQATAAVNNALQAQVIA
                     LKAYYGLLIDAHLIWGLEHD"
     gene            9734..9895
                     /locus_tag="PLD53_04550"
     CDS             9734..9895
                     /locus_tag="PLD53_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC60210.1"
                     /translation="MLLSQGIGAETQRPLATVVVGGLLTSTALTLLLLPLMYEWVEAR
                     RERRTVTSP"
     gene            9907..10332
                     /locus_tag="PLD53_04555"
     CDS             9907..10332
                     /locus_tag="PLD53_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60211.1"
                     /translation="MKTVFAIAGLAVLALTGCVGHTHDLAPESGNPEATIGTFTHEGR
                     EGPAMVLEFKGQRFAAQGFAIRRDANIAALQQHDRRSMRPSWVSAGSNPEHYEYSATP
                     ELRATDGAVLRCTLTWPARQAPAGYCETREGTRIAVRFD"
BASE COUNT         2120 a         3110 c         3219 g         2065 t
ORIGIN      
        1 cgccccgacg ggccgccatc aacaaggcgg ctgaagcgcc agttggactg cgcatagcag
       61 caacgataac gttagtgtcg agaacaagcc tcatatcatc agcaatatca tattgcgggc
      121 gatgtgtcta caagaaagca gatcatggcg ccataacacc accccgcgta actatcgata
      181 aaataggtta tcaatagtca aatgaccgga agctgttgcg cacgctcgat cacatttgta
      241 ttagtatcta atacacaaga tcaacccaga aaggagccgt catgccgcga aacaccagcg
      301 tcaccctggg tgaacacttc gagggcttca tcgcccagca gatcgatgcc ggccgcttcg
      361 agtccaagag cgaggtggtg cgcgccgcga tgcggctgct cgaagagcac gaacagaaag
      421 tgagcgcctt gcgtcaggcc ctggttgagg gcgagacgag cggcgaaccg gtgcccttca
      481 cgatggcgtc gatcatcgag gaggcacgcc aggagtgtta caagggagcg caaggctaga
      541 tgccgattat cggccatctg gctgaagccg gcatcgtgat cttcgacgcg tttcgtgaag
      601 gcaacatcgc cccggcaaca cagaacttcg aattcatcca ggcctgtgaa gcacgcctgc
      661 ccaaagggca tcggatggcc catgtccgcc tggactcggc cggctatcag gctgacattg
      721 tcaattactg cgaaaacacc ggcaagacct tcgccatcgg cggtcggctg gatgcgccga
      781 cacaacaggc cattgccgga atccctgaaa gcgactggaa gcgctacgct gactgcgccg
      841 ttgccgagac actgcacagc atgaacgaga ccaggaaagc gttccgcctg atcgtcgtca
      901 agtacaagcg gcaggcagaa ctgttcgatg acgcaccgag ataccacgtc atcgtcagca
      961 actgcgtcga atcgaccacc gacaccctga tctggtatcg ctagtgcggg gagacttccg
     1021 agaatgggat caaggaactg aagatcggct tcggcatggg gcgcatgccc tgcgggcagc
     1081 ttatcgtcaa cgctgcattc ttccgcatcg gcgttatcgc ccataacctg ttcgtcctgt
     1141 tcaagcattc caccctgggc gccgattggc agcgctataa agtcgccacc atccgctggc
     1201 gcctgtttca tctgccgggc aaggtggtgc gccatgctgg ggcatgggtg ttgaaggtcg
     1261 cggtcgatac cgtcgaccgc ttccgcacca tccgtacccg cagcttcgcg caggccgtgg
     1321 caaattctcc atagccgctt ccgctcggca ccccggcaag actcctccgt gcagcactgc
     1381 gcgaggaacc gttcgtccag aactcgtgcc accaccatgc tatcgggatc cgcccctccg
     1441 cttttgcgta ctctgcgaac tgccgccgcc tcttgcgtgg cccctctcgc acttcgcgcg
     1501 ccttcagcat gggcaagagc cccggcaacc gggcggcgag gttttcaatc gcgggatttg
     1561 gggcctttta atggcttgac gtatcctatc cccctggata ggatacgagg tatgagcacg
     1621 cacacttccc atccagagat catcaagcgc ctgaaacggg ctgaaggcca cctcaggagc
     1681 accatcacca tgcttgagga cagccgtgac tgtctggcga tcgcccagca actgcaggcc
     1741 gtcgaaagcg cagtcggcaa cgccaagaag acgctggtgc acgaccacat cgaccactgc
     1801 cttgaacaag cagtgcgcga cgcgccgcaa agcgcggaag agactatccg cgagttcaaa
     1861 gccatcacca aatatctctg aaggaaacac gatgaccagt ttttccaccc tgctggcgca
     1921 gggcgcggga aacgcctggc tgtttatccc gagtgcgatc ttgctcggcg gcctgcacgg
     1981 cctcgaacca ggccactcca agaccatgat ggccgctttc attgtggcga ttcgtggcac
     2041 ggtggggcaa gccgttctgc tcggggtgtc ggcgacgatt tcacacacag ctgtggtgtg
     2101 ggtgatcgcg cttggcggca tgtacttcgg ccagaaatgg gacgcggaaa ccagtgagcc
     2161 ctatctgcag gtggcgtccg ccttggtgat catcggcgtt gctctctgga tgatctggcg
     2221 gaactggcga caacagcagc ttgccgagca ggaccacgat cacgatcatc atcatgacga
     2281 cagcaaacgc atcgataccg gtcacggcgt ggtacgcctg gatgtgttcg aggacggcgt
     2341 gccgccgcgc ttccgcttgt tcgcagaaag cgggcacggc cacacctgga cagccgatca
     2401 ggtgaggatc gaaaccgaac gaccagacgg tagccgccag tcattccggt tcgtgcagcg
     2461 cgacggcttt gtggagtcgg agcaggaaat tccagagcca cacgaattcg tggctcgtct
     2521 gcgcctgggt catgagcacc acagccacga ctatgatgtc gagttcgtcg agcacgacca
     2581 ccagcaccac gccatcaagg actacgaagg gctggatgtg tcagctcccg gataccagga
     2641 tccgcacgaa ctggcgcatg ccaacgatat ccgccaccgt ttcaccaatc gggaagtgac
     2701 gaccgggcag atcgtgatgt tcgggttgac tggcgggctg attccctgtc ccgcctcggt
     2761 taccgtgctg ttgctgtgct tgcaactgaa ggagatcgcc ctcggtgcga ccctggtgct
     2821 gagcttcagt atcggcttgg ccttgacgat ggttctttcc ggcgccttgg cagccttgag
     2881 cgtgaaacac gtttccaggc gctggagcgg tttcggcaag tttgcgcgga gagcaccgta
     2941 tttctctgct gcgctcatcc ttctcgttgg tctttacgtt ggctaccaag gcctgaaagc
     3001 actggcctga cgagtaggct gccgttagaa gatatgaacc acctctccat taggcagaat
     3061 gtacccctcc caggataaga tgccgccatg aaagaaggag caattcatcc atcgcactcg
     3121 tgtatcaaca agcggctaga gaaagtcgaa ggccacttgc agatcatggc ggaatgatgg
     3181 aagctggccg ctgttgtttg agtgtctcgc agcagttgca cgctgttgag aagatgctca
     3241 ctgcggaaaa aggccctcgc gcatgaccac ctcaatcatt gtctcgaacc caccgccgat
     3301 cacgctgcgc ccaggcagtt cggctgcact cgatgacttc agaaaaatca taaagtatct
     3361 gtaatgcaca ggccgcatcg aaaaaccata cggctactaa tgctcatcct tttttttgtg
     3421 atgagcgtcg gcgcctatgg cttcaacttg aagcggtttt cacacgaact tgatcacgat
     3481 cgccaaacgc gcaccgcgtc gatcaatcac gatcacgcgc cgcggcttga tgccaagagc
     3541 aatccggccg ccgaaccatc gaacgatgcc gaacatgaac tgttgcacgc cttgagccac
     3601 tgcgagctat ttcccaattc ggctgtcagt caccttgctg agccgcctgc tcggacagcc
     3661 ctcgtgctac cgagcgtcct cactctgctc ccggcagaac tcgagccccc tttccgacct
     3721 ccccgtagcg catccctcgt ttaactccgc gtcgctcgcg gagttccgca ggtttgtctt
     3781 tcccctggct cgacgagcgg cgtggtcacg cttgttgtgt cggcagacgc tataaagagg
     3841 ttgataatga aaacaagcag ctcccccttc ccggcgggaa gcgcatgggc aatggccgtg
     3901 atgtgcatcc tcacggctac cacgcaggca gccgaccggc cagcaaaatt caccgttcca
     3961 aacagccaga ttcaggcgtt gggcattcaa accacgccat tgcagaatga taccgacttg
     4021 gtaaaggcga gttatcccgc acatgtcgtg gtgccgccag aagcggaaca ggtgatcagt
     4081 tcgcccgtcg ccggcctagt agcgcaattg ctggtgcagc aaaaccaggt cgtgccttca
     4141 ggcgcaccac tggtgcgcat cgtcagtccc gaactgggcc agttgcagct gcagttgtta
     4201 caagcaaccg ttcgcgccac ccttgcacgg caagcggcgc agcgcgagca cgctttgttt
     4261 gatgaaggaa ttattgccaa acgtcgagtg caggaagcgc gagcggcgct gcaggaagcc
     4321 gaagccacgc tcaatcaggc caaggcagcg ctgcgtctaa gcggcatgcc gtccgcgacg
     4381 atcgagcgca ttgcgacgtc tggcaagccc caagacagcg tgactctgct cgcgacgcag
     4441 gccgggatcg tcaccgttat cgcggtcaaa cctggccagc gagtcgaggc agcgacggct
     4501 ttgttgcacg tggctcaaac ggactctctg tggctcgata ttcagcttcc ggtgtcggaa
     4561 cgtgtgggct ggccagccgg aacccaggta aaagtgctcg gccgcagcct cacggcgcga
     4621 atcttgagcg taagcccgac cgtctcccac ggcagtcaga cagtagtact gcgggcagcc
     4681 gtcgaaggca agaccggcga ggttcgaccc ggcgagttgg taactgtcga actgccaatc
     4741 accgctactc aaggcaattg ggatgtgccc ttgtccgcgg tggttcatga cggcgaacaa
     4801 gcttacctcc tcgtccgcac cgcggacggt ttcgaagcca gacccgtgaa ggttgtggtt
     4861 agcgctggtc agcgtgtgcg cgtacaaggc ccgctcaaag aaggtgaaca gatcgccatc
     4921 agcggcgtgg tagcactcaa gggcgcctgg ctcgacgcga aggagagcaa gtgatgcttg
     4981 ctcgtctcgt tcagctttcc ctgtcgcagc gcctgttcgt cttgttggcg acgctgattg
     5041 tcgctggtgc gggatggatg gcctacctcg gcttgccgat tgatgcattc cccgatgtgt
     5101 cgagcacgca ggtcaaaatc atcatgaagg cccccggcat gaccccggag gaggtcgaaa
     5161 gccgaatcgc ggtgccgatc gaagtagaaa tgctcggcat cccgaaaacc aaaatcctgc
     5221 gctcggtcac caagtacggc ctggtcgacg taaccatcga cttcgaggac ggtaccgaca
     5281 tctactgggc gcgccagcag gtgagtgaac gcctgggcgg cctgacggac agtctgccgg
     5341 ccggcatcac gggcggaatg gcgccgatca ctaccccgct gggggaaatg ttcatgttca
     5401 ccgtcgacgc cgatggcttg tcgctggccg aacggcgcag cctcctggat tgggtcatcc
     5461 ggccagcgtt gcgcacagtg cctggcgtcg ccgacgtgaa tgcgctgggt ggcgtcgttc
     5521 gcgccttcga ggttgtgccc gatcagatca agatggctgc cagcggcgtg acggtggccc
     5581 agttgaagga ggcgattgac gccaataacc gcaacgatgg cgcgggccgg ttgggcgaag
     5641 gcgacgaagt attgctggtg cgcagcgaag gaagtgtcac gagcctggac gacctgcgtg
     5701 cgatcgtggt gacgatcaag gacgattcgc cggttcgtct gggcaacctg gccgaggtca
     5761 agatcggcac tgtcacgcgc tatggtgtgg tgacgcaaga tggcaaagcc gaagccgtcc
     5821 agggactggt gctgggactg gcaggtgcca atgcgcaaaa agtcgttgaa ggcgtgcgca
     5881 agaaactgga tgaactgcaa ccaacgctcc cgaaagatgt gcagctcggc gttttctacg
     5941 atcgcgcctc cctcgtcagc aaggcggtcg gtgcggtctc ggcagcgctg ctcgaggcga
     6001 cagtcctggt catcattttg ctggggctgt tcctgggcaa tatccgcgcg gccgtgaccg
     6061 tggctctagt actgcctctg gcggcactga tcacctttat cctgatgcgc ggcttcggta
     6121 tgtcggccaa cctgatgagt ctgggaggcc tcgcaattgc tatcggcatg ttgctcgacg
     6181 cggcagtggt ggtggtcgaa aacatcgtac agcgcctggc gaccgacccg acggccggca
     6241 agctgccgcg cttgcatacc atctatcgcg cagtgcgcga ggttgcggtt ccggtctcgt
     6301 ccgggatcct gatcatcatt accgtctttc tgccgctttt gacgctacag gatctggaag
     6361 gcaagttctt cgtgccggtg gcactgacca tcgtctttgc cctggccggg tcgctgctgc
     6421 tttcgctaac cgtcattccg gtgctggcgt cgtatttgct gcgcgaggtc aagcatgagg
     6481 atccgtggtt gccacgcaaa ttgctggcac tctatgagcc ggccctagcc tggggactgc
     6541 gacggcaggg tatcgtcgtc ggcgtggcag tgctgctact gctgggagcg ggcggggtgt
     6601 acacccaggt tggcaagacc ttcatgccga cgatggacga aggcgatttg atcgtcggta
     6661 tcgagaaatt gccctccatc agcctggaac aaagcgccgc tctcgatctg aagattcacc
     6721 aggccctcat gaaggccatt ccagaggtca ccggcatcgt tgcacgtgcc ggctctgacg
     6781 aaattggcct cgacccgatg gggctcaacc agaccgacac ctacctcgtg ttgaagccgc
     6841 gagacgactg gcgcatgaag agtaaggaag cactgatgga cgagatacgc aaagtgctcg
     6901 accccatgcc tggtatctcc tacagcttca cacaacccat tgaaatgcgc gtctcggaaa
     6961 tgattatcgg cgtgcgcggc gatctggcag tgaagatatt cgggcctgat ctcgaccagc
     7021 tcaatacgta tgcgagcgag gtcgaagcac ttctcaagac ggtcccgggg aatcaagacg
     7081 tctacaccgt ccagaacgat ggtgtgcagt acttgcaggt ggtggttgac cgcctgcagg
     7141 caggtagcct tggtttgagc gtggaagaga tccaggacgc gctgcgcacg cagatcgagg
     7201 ggcagcgggc cggcatcgtg atcgaaggta accgccgcac acccatcgta ttgcgcggca
     7261 ccgaggcggt tcgtatgtcg ccggctgaat ttggcgccat gcggatcacg acgcagggcg
     7321 gcagcacggt gccattgacc agcgtcgcca agctgcaacg ggcggcaggg ccggtcaaga
     7381 tcgatcggga aatgggcagc cgttatagcg tggtcatcgc caacgtcggt ggccgcgatc
     7441 tggttggctt cgttgaagag gccagagcgc tcgtcacgga aaagttgtcc ctgcctaccg
     7501 gctaccgcat cacctggggg gggcagttcg agaaccagca gcgcgcggct gcgcgtttgg
     7561 cggtggtagt tccggtcgca cttggcctgg tcttcgtcct gctgttttcg accttccgct
     7621 cggtgcgcca ggctttgctc atcctgagca acgtcccatt tgccctggtc ggcggtatcg
     7681 tcgccttgtg ggtgacggga gaatacctgt cggtgccagc gtcggtgggc ttcatcgcgc
     7741 tgctcggtat cgcggtcttg aacggagtcg tgctggttag ctatttcaat caattgcaca
     7801 gcgaagggat gaaattgagc gatgtcgttg ttcagggcgc gaagcggcgt ttgcgcccgg
     7861 tactgatgac cgcctcaatt acggcgttcg gattgatccc gctgctcttt gccagcgggc
     7921 cgggctcgga aatccagcgg ccgcttgcca ttgtcgtgat cggtggcctg accaccgcca
     7981 cggtgctgac gctgatgctg ttgcccatcc tgtatttgcg ctttgctttc ccaagacgag
     8041 aggttcccca tgtctgaact ttgcttgacg ctggtgtgtc cgccagaggt cgaagaaaaa
     8101 ctactcgatc tgctgctcct gtggcccggt gcgacgatct tcaccagtac agcgacagcc
     8161 gcgcacggtc tggcccacga gtctctggat cagacagaac aggtgctggg tcgggcgcga
     8221 gccacggagg tgcaggtgat ctgtgcggcg gctggtcaag cggcgctgct tgcagcgctg
     8281 cgtcagcaat tctccggcgt ggggctgcgc tactgggtga ccccggtcgt cgaagcggga
     8341 gaaatcgcat gatggttaga ttgctcttga tcggcatctt cgccgtagtc aaggccgcat
     8401 ctgcggcgca gccaatgaat ccgccggggc tcatgccaac cgcaattgcg cgccctttat
     8461 tcgagcagga cccgggcgtc gccgcggcgc gggcaggctt ggacgtggct ctgcaagagg
     8521 cgagcattct cgatcaatct ccgtatgagt ggaccgctcg cgttgatggg cagcagcgca
     8581 gagtggaaaa cggtccccgc tacaacgaat ggttcgtagg tgtcgagaag accattcggc
     8641 ttcccggcaa agccacagcc gatcgcagta tcggcaaggc aaccgtcgac gagtctcggg
     8701 cgcgctatgg cgaggctttg ctcacatcgg ctcgtgaact gatggaattg tgggtggact
     8761 ggctggcggc ggaacgcgct cgcgagctgg gtgggagcag ccttcagtcg atgcaagcca
     8821 gtctcgcggc cgtggagaaa cgcgctcgcg ccggcgatgc ctcgaaattg gacctgggca
     8881 ttacccgcgc cgaactggcc gagcaacgac gcatggacaa tgacgcaaag acgcaggctg
     8941 ccgcagcctg gttacgtcta tccacccgct ttcctggcgt cgagcgtcag ctcttggctc
     9001 tgcccgagcc gttggtgctt gggagcgagg cgccatcttg gcaggagcgc atcctggctg
     9061 aaacgcatgc actgaagctt gtgcaagcgc aagtccggaa agcgcaagcg caggcagaac
     9121 gagcacgtgc ggagaagatt cctgatccaa cgttcggggt ctatacggct tcggagatcg
     9181 gcggccgcga gcgcattccc ggagtcacaa tcagcattcc gattccggga ggcgcgcgtg
     9241 acttgcgcag tgcacaggcg attgcggcag ttgaggtttc gcgccaggaa gccgaactca
     9301 aacagcgcca gcttcaggca gggattgcaa gcgccgtggc gactgcgcgc ggtggctacg
     9361 agagctttca gatcgccaac gaaggcgcgg cggctatgca agagaacgcc cgtctgatgc
     9421 agcgggctta tgcgctcggc gaagcggagc tacaagcttt tctgctgacg cgtcgccaag
     9481 cgacggctgc ggtgaacaac gccttgcaag cgcaagtcat cgccctgaag gcctattacg
     9541 ggctgctcat cgacgcgcac ttgatttggg gcttggagca tgactagcca gtccgtgtct
     9601 tcacttcgcc agaagaattt tttgcgtact tttgaagctc cgtcgatgat tgaaagatgc
     9661 aacatgcaaa ataccgtaac cataggctta gggcgtacga acgatgaaca cctcttcgag
     9721 ttatgcgtca tgagtgctgc tctcgcaggg tatcggagcg gaaacgcaac ggccgctggc
     9781 gaccgtggtt gtcggtggtc tgctgacatc gacggccctg acactgctcc tactgccgct
     9841 catgtatgag tgggtcgaag cgcgccgaga gcgccgcacc gtaacttccc cttgagaaag
     9901 acaacgatga agacagtgtt cgctatcgca ggtctggccg tgcttgcgct caccggttgc
     9961 gtgggacata cgcatgatct cgcgccggaa agtggaaacc cagaggccac aatcggcacc
    10021 ttcactcacg aggggcgtga ggggcctgca atggtcctgg aattcaaggg acagcgtttt
    10081 gcggcccagg gctttgcgat acgccgcgac gcaaacatcg cggccttgca acagcacgat
    10141 aggcgcagca tgcgtcccag ctgggtatcg gctggaagca accccgaaca ctacgagtac
    10201 tcggccacgc cagaattgcg tgccacggat ggcgcggtgc tgcgatgcac cctgacctgg
    10261 cccgcccgcc aggcaccggc gggctattgc gaaacgcgcg aggggacgcg cattgccgtc
    10321 cgcttcgatt aagctggtcg tcaatcacga aggggttgta agccggaacc gtcgaattcc
    10381 gtcatcccca cgccggccct gccacgagcg tatccaggtc caggtcgatc accgtgccgt
    10441 cgctttggaa cgtgtagtac ccgttccaca tcggtgattc cgctcgccgt atccttcatt
    10501 tccttgtcac ggag
//
LOCUS       DAORPM010000061        10486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1440_length_10486_cov_10.144281, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000061 DAORPM010000000
VERSION     DAORPM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1440_length_10486_cov_10.144281"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..647
                     /locus_tag="PLD53_04560"
     CDS             213..647
                     /locus_tag="PLD53_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC60212.1"
                     /translation="MTMTTATATLNIKALQKTWAAFDRIAHLRPIHTEDEYDRTVSLM
                     NNLLDMVGDQEDHALSGLLDLVSELVADYDTKHFAIEASEPNEVLRYLIELRGLKQGD
                     LAEIVPQSNLSAILSGKRKISATLAGKLAKFFNISPAVFVPG"
     gene            complement(901..1482)
                     /locus_tag="PLD53_04565"
     CDS             complement(901..1482)
                     /locus_tag="PLD53_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRC60213.1"
                     /translation="MASKKILMLTGDYTEDYETMVPFQALLMVGHTVHAVCPGKNAGA
                     TVRTAVHDFEGDQTYSEKPGHNFTLNASFDSVTADDYDALVIPGGRAPEYLRLNPKVI
                     AMVQHFANAKKPIAAICHGAQLLAAAGVLGGRSCSAYPACAPEVTAAGGSYAEIPVDQ
                     AHVDGNLVTAPAWPAHPDWLAKFLKVLGTRIEP"
     gene            complement(1594..1947)
                     /locus_tag="PLD53_04570"
     CDS             complement(1594..1947)
                     /locus_tag="PLD53_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60214.1"
                     /translation="MKSALIFAAVAEIATGLALLIVPLLVGRLLLGDELTGVALPVAR
                     VAGIALIALGVACWPGPPRVGMLLYGAVVTLYLAYLGLTGSATGIVLWPAVILHAVLT
                     GLLIRQPAQDKSRRA"
     gene            complement(1963..4434)
                     /locus_tag="PLD53_04575"
     CDS             complement(1963..4434)
                     /locus_tag="PLD53_04575"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRC60215.1"
                     /translation="MASGPTSAQQVTGTLGSPGATTTISGKQLPPPDPKFGGVIKEKA
                     SESAPWWPPRVVPPKGAPNVLLIMTDDDGFGAPSTFGGVIPTPALDRIAKSGLRYTNF
                     HSTSLCSPTRAALITGRNHHSVGSGVVGEIATGYPGYDSIIPIEKGTIGTILKHNGYA
                     TSWFGKNHNTPFFANSQAGPFEQWPNGMGFEYFFGFVGGDASQWEPNLFRNTTPIYPF
                     EGNPGWNLTTAMADEAIQYMKQLKELAPKKPFFVYYVPGGTHAPHHPTKEWIDKISAM
                     HLFDDGWEKLRDTIFANQKKLGIMPENAQLTPWPDGQDVYGGAKLPRWDTLDFEQKKL
                     FIKQADVYGAYQAYTDHEIGRVIQAVEDMGELDNTLIIYISGDNGASAEGMVNGTPNE
                     FTTFNGIEVPIKDQFLWYEFWGSERTFPHYSAGWAWAFGTPFKWMKQVPSHFGGTAQG
                     MAISWPGHINDAGGVRRQFHHVIDIVPTILEATGIPAPDTINGIKQLPIEGTSMAYTW
                     DKANANAPTRHTTQYFEMLGNRAIYHDGWVAATTPATRPWELSSAKPPDVITGYKWEL
                     YNVVEDPTQNNDLAAKMPEKLKQMQNIFYVQAAKYNVLPLDNTTLARWNTPRPSLKAG
                     RTEFTYSGTLSSVPASTAPNILNKSYTITAEVTIPEGGAEGMIVTQGGRFGGYGLFLS
                     KGELGIGRGKVVFLYNLLDLKRTTWEGPELAAGKHTIVFDFKSDGPGLAKGGTGVLTV
                     DGKEVARNSMEHTTPITFPEDETFDVGMDTRTPLALIEYRYDSPFKFTGTINKLTVKL
                     EPFEPEQGAKAGATSSPGPGSAARR"
     gene            complement(4585..5565)
                     /locus_tag="PLD53_04580"
     CDS             complement(4585..5565)
                     /locus_tag="PLD53_04580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRC60216.1"
                     /translation="MKPIHSIFVLLALFFNTGVQADPVRLIFVGDVMLDDGPGRVVAS
                     GRDPLVFFDAALRDADFTIGNLECPIATVGKPLDSKIVSFRADPRVVPLLKGRFDALA
                     VANNHSGDYGKEAFLETIGHLDRADIAYFGGGRDLAGAHAPLWIERRGLRIAVLGYNE
                     FKPRSFEAGPDLPGIAWSEDSQVIGDIRAARAAGADLVIPFMHWGWEREPNPSERQRQ
                     LARSMIDAGADVVVGGHPHVTQGAEYYRGKLIVYSLGNFVFDGFELPAAKRGWLLRLT
                     LDRQGLVEWETLAAQMDEEGTPHPVPGALTPCGKLDRGTEADRVQACANP"
     gene            complement(5562..6026)
                     /locus_tag="PLD53_04585"
     CDS             complement(5562..6026)
                     /locus_tag="PLD53_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60217.1"
                     /translation="MRVSGQSVPYFSGCVLAGLLAIASTSAHAEQVVCHYTYGGETKR
                     LVADPASSPYAIKGIQIGSYFRFRVVFTDKPADIASISIYTYADLDEGPVLIHQAIYP
                     YPPTRSSDSRYGFSGLHFVYEPVRDGELQYWCAMTNPESSAPAGRPAASTVR"
     gene            complement(6060..6797)
                     /locus_tag="PLD53_04590"
     CDS             complement(6060..6797)
                     /locus_tag="PLD53_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC60218.1"
                     /translation="MQRILIIGSTSAIAEATARLWAARRDTLFLVGRKPDRLDVIAAD
                     LRVRGATSVGCYTMDACDTPAHAAMLEAAVSAMGGLDVALIAHGSLPDQKACEVSVDQ
                     TLKEIDTNGLSVIALATLLGEKFAAQGSGSLAVIGSVAGDRGRQSNYVYGAAKGMVSL
                     FLQGLRNRLAKQGIQVLTIKPGFVDTPMTAAFSKGALWAKPDDIARGIVRAVDRKSDE
                     VYLPGFWWVIMTIIRHIPERIFKKLSL"
     gene            complement(6814..8109)
                     /locus_tag="PLD53_04595"
     CDS             complement(6814..8109)
                     /locus_tag="PLD53_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC60219.1"
                     /translation="MVLQAWGQLPATPAAEERWWADRDADLPRTDTSLLAHGNGRSYG
                     DVCLNSGATLLHTRGMDRFIDFDDTSGTLVCEAGILLSDILAVFVPRGWFLPVTPGTR
                     FVTLGGAIANDVHGKNHHRAGSFGCYVQRFELLRSDGSRRVCSPAENADYFAATIGGL
                     GLTGLITWVAISLKRIASPMISVENTRFDGLDDFFTLNAAAEAAHEYTVAWIDCLASK
                     PRGIYMAGDHAKAGDPGVESGKPLSVPLTPPFSLINGLSLHAFNQAYYHRPLAARTSM
                     SYAPFFYPLDGVLHWNRIYGKKGFYQYQFVLPLAERAALDDVLRGIASSGQGSFLAVL
                     KTFGALPSPGLLSFPMPGVTLALDFPNRGASTLALFDRLDAIVSAAGGRLYAAKDARM
                     SGEFFRRSYPRIDEFQRFVDPRFSSDFARRVLLAGSAVT"
     gene            complement(8109..9077)
                     /locus_tag="PLD53_04600"
     CDS             complement(8109..9077)
                     /locus_tag="PLD53_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRC60220.1"
                     /translation="MPRADAMADSSQASPAERNWPLRAALWSVVFAAVGYLALSLWVG
                     WRDVLAALVQVGPWVLLGLLALSLSNYVLRFVRWSRYLALLDVSLPWRNHLAIYFSGF
                     ALTTSPGKLGEMLRSVLLKPYGVPAASSLAAFFAERASDLLSIIVLAAIGLWVYVPAR
                     PVVGLALLAVCLVLVLAQWTAGIAAVDRWAMPRPQKWAKLLVRLCEIVLHFRRCFSLP
                     AMSMGLIIGVLAWFAEGFGFWWLLAEIGHPLPLTTAVFIYAFAMLVGALSFLPGGLGS
                     SEAVMFGLLVLNGVPEVTAVTATLICRLATLWFAVALGAACLARQR"
     gene            complement(9098..9565)
                     /locus_tag="PLD53_04605"
     CDS             complement(9098..9565)
                     /locus_tag="PLD53_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60221.1"
                     /translation="MTNNFSLPSFAVAAIVSLALSACAPNAWQSPLDGPVSNAVDKID
                     NQLADCAPGEGQSGAADAFMTLAQNKCWGKHIGSMMISNNMSSYDSSPYFMDLTSRFY
                     CGAIDQASYVNSLESFLNASANQDGVVCIIDLAARENLRTPQQRPGSLSAPPQ"
     gene            complement(9614..>10486)
                     /gene="mnmA"
                     /locus_tag="PLD53_04610"
     CDS             complement(9614..>10486)
                     /gene="mnmA"
                     /locus_tag="PLD53_04610"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRC60222.1"
                     /translation="ERVFAEFLKEYQAGRTPNPDVLCNSEIKFRAFLDHAMQMGAEKI
                     ATGHYAGVREFAGRFQLLKAEDGTKDQSYFLHRLNQAQLSKTLFPLADIYKRDVRKMA
                     EAAGLHVAEKKDSTGICFIGERPFKEFLMRYLPPKKGEIRCLDDDRVVGEHDGLTYHT
                     LGQRKGLHIGGTKTYAKEGEHDAWFVAKKDMEKNILYVVQGHDHPALLCDVLRAEQLS
                     WIAGDGVDDLPRTHWVYCAKTRYRQPDAPCEIEHIDANACEIQFAEPQWAMTPGQSVV
                     VYESRVCLGGGIIC"
BASE COUNT         2199 a         3259 c         3029 g         1999 t
ORIGIN      
        1 gaaatattaa gaactgcaaa ataagagaaa tttgccgctc ggatcaatga atgccgccaa
       61 gcgacaaggc cgaacgaacg tcggcaatcg agagacctat tcagaggcaa ctcctggccg
      121 gatggagcca aatctgaaat ctgattcgtg cgttgggtaa tgactcaccg ggaatacgac
      181 gactggtgca aacgatacag gaaaggcgag gcatgaccat gacaactgcc accgctactc
      241 tgaacatcaa ggcgctgcaa aagacctggg ctgcgtttga tcgcatcgcg catttgcgtc
      301 cgatccatac cgaggacgag tatgaccgta cggtttccct gatgaacaat cttctggaca
      361 tggttggtga ccaggaggat cacgccttgt cagggcttct tgatctcgtc agcgaactgg
      421 tggccgacta tgacaccaag cacttcgcca tcgaagcttc cgagcccaat gaggttctgc
      481 gctacctgat cgaattgcgc ggcctgaagc aaggcgatct cgcggaaatc gtcccacaga
      541 gcaacctttc agcgattctc tccggcaagc gaaagatcag cgcgaccctg gctgggaaac
      601 tggccaaatt cttcaacatc agtccagctg ttttcgtgcc tggctaagaa ggtactgcgc
      661 gataccggtg cggcatcctg tgcggcaagt atccgccgcc agctgcccgc cattcaaaac
      721 tgggttgaaa actgcattgt gttgccgctt cggccgtcca ctcctgggcg aacatcaggg
      781 gtgctcctcc aaagaaagaa cagagcggtg gtcgatgcga gcttgaaaac gaaaaaaggg
      841 cacttcagaa gaagtgccct tttgcatgcg tgactgtcgc gccgcgtcaa cggcctgatt
      901 tcagggctcg atgcgcgtgc cgagcacctt cagaaatttc gccagccaat ccggatgcgc
      961 cggccaggcg ggcgccgtca ccagattgcc atcgacatgt gcctgatcaa cgggaatttc
     1021 ggcatagctg cctcctgcgg ccgttacctc cggagcacag gcggggtagg cactgcatga
     1081 acggccgcca agtacgcctg ccgccgcaag aagttgtgcg ccgtgacaga tggcggcgat
     1141 cggctttttc gcgttggcaa agtgctgcac catggcaatc actttcggat taaggcgcag
     1201 atattccggc gcccgtccac cgggaatcac cagcgcgtca tagtcgtcgg ccgtcaccga
     1261 atcgaacgaa gcgttgagcg tgaaattgtg tccgggcttt tcgctgtagg tctggtcgcc
     1321 ctcaaagtcg tgcaccgccg tacgcacggt tgctccggca ttcttccccg gacagacggc
     1381 atgtaccgtg tgcccgacca tcagcagcgc ctgaaacggc accatcgtct cgtagtcctc
     1441 ggtgtagtca ccggtcagca tcagtatttt cttgcttgcc atgatatttc ctccttgttg
     1501 agtgggtccg acggatagga ttgtgaatca accactgcgc tgcgactact attcagcaaa
     1561 cgaactcaac cggacccgat tgcaatactt actctatgct cttctcgact tgtcttgtgc
     1621 cggttgccga atcaagagcc ccgtcaggac ggcgtgcagg atgacggccg gccaaagcac
     1681 gatgccggtc gcactacccg tcaaaccgag ataggcgaga tacagcgtaa cgaccgcgcc
     1741 gtagagcaac atgccgaccc gcggcgggcc cggccagcag gcgaccccaa gggcaatcag
     1801 ggcgatgccg gccacgcgtg cgacgggcag ggccacgccg gtcagttcgt cacccaacaa
     1861 caagcgcccc acgagtagcg ggacgatcag caaggccaag cctgtcgcga tttctgccac
     1921 ggcggcgaaa atcagcgccg atttcatggc gcggaccccg ctctaccgcc gtgcggccga
     1981 ccccggccct ggactgcttg tagcgcccgc cttcgcgccc tgctccggct cgaaaggctc
     2041 aagcttgacg gtcagcttgt tgatcgttcc ggtgaacttg aacggcgagt cgtagcgata
     2101 ctcgatcagc gccagcggcg tgcgcgtgtc catgccgaca tcgaaggttt cgtcctccgg
     2161 gaatgttatc ggagtcgtgt gttccatgga attcctggcc acctctttgc cgtccaccgt
     2221 gagcacccct gttccgccct tggccaggcc ggggccatcc gacttgaagt cgaagacgat
     2281 ggtgtgcttg ccggcagcca gctccggccc ttcccaggtc gtgcgcttga ggtcgagcag
     2341 gttgtagagg aacaccacct tgccgcgacc gattcccagc tcgcccttgc tcagaaacag
     2401 cccgtatccg ccgaaacgcc cgccctgggt gacgatcatt ccttccgcgc cgccttctgg
     2461 aatcgtgacc tcggcggtga tcgtgtacga tttgttaaga atgttgggcg cagtgctggc
     2521 cggcacgctg gacaaggtgc ccgaataagt gaattccgtg cggcctgcct tcaggctagg
     2581 acgcggcgta ttccagcgcg cgagcgtcgt gttgtcgagc ggtagcacgt tgtacttcgc
     2641 cgcctgcacg tagaagatgt tctgcatctg cttgagcttc tccggcatct tggccgccag
     2701 gtcgttgttc tgcgtcggat cctccacgac gttgtacagc tcccacttgt agccggttat
     2761 cacatccggc ggtttcgcgc tgctcagctc ccacgggcgc gtggccggcg ttgtggccgc
     2821 tacccagcca tcatgataga tggcgcggtt gccgagcatc tcgaagtatt gtgtggtgtg
     2881 ccgggtcggc gcgttggcgt ttgccttgtc ccaggtatag gccatgctcg tgccttcgat
     2941 cggcaactgc ttgatgccgt tgatcgtgtc aggcgccgga attccggttg cttcgagaat
     3001 ggtcggaacg atatcgatca cgtggtggaa ctgccgacgg acgccgcccg cgtcattgat
     3061 gtggccgggc catgagatgg ccatcccctg tgccgtgccg ccgaagtgcg acggcacctg
     3121 tttcatccac ttgaacggcg tgccgaaagc ccaggcccag cctgccgaat agtgcggaaa
     3181 ggtccgctcg gagccccaga actcgtacca gaggaattgg tccttgattg gcacctcgat
     3241 gccattgaag gtggtgaatt cgttcggcgt gccgttgacc atgccttcgg cactggcacc
     3301 gttgtcgcca ctgatataga tgatcaaggt gttgtcgagt tcgcccatgt cctcgaccgc
     3361 ctggatgacg cggccgatct catgatcggt gtaggcctgg taggcgccat agacgtccgc
     3421 ctgcttgatg aacagctttt tctgttcgaa atcgagcgta tcccaccttg gcagcttggc
     3481 gcctccatag acgtcctgtc cgtccggcca tggggtcagt tgcgcgttct cgggcatgat
     3541 gccgagcttc ttctggttgg cgaagatggt gtcgcgcagc ttttcccagc cgtcgtcgaa
     3601 caaatgcatg gcactgatct tgtcgatcca ttccttcgtc gggtggtgcg gtgcgtgcgt
     3661 gccaccgggc acgtagtaga cgaagaacgg cttcttgggc gccagctcct tgagctgctt
     3721 catgtactgg atcgcctcgt cggccatcgc cgtcgtcagg ttccagcccg gattgccctc
     3781 gaaggggtag atcggcgtgg tattgcggaa cagattcggc tcccactggc tggcgtcgcc
     3841 accgacgaag ccaaagaagt actcgaagcc catgccgttc ggccattgct cgaaaggccc
     3901 ggcctggctg ttggcaaaga aaggcgtgtt gtggttcttg ccgaaccatg aagtagcgta
     3961 gccgttgtgc ttcaggatgg tgccgatggt gcctttctcg atcgggatga tcgagtcgta
     4021 gccgggatac ccggtggcga tctcaccgac cacgccggaa cccaccgagt gatggttgcg
     4081 tccggtaatc agcgcggcgc gtgtcggcga acacagcgaa gtggagtgga agttggtgta
     4141 gcgcagcccg ctcttcgcga tgcggtccag cgccggggtc gggatgacgc cgccaaaggt
     4201 cgacggcgct ccgaagccgt cgtcgtcggt catgatcagc aggacgttcg gtgcgccttt
     4261 cggcggcacg acgcgtggcg gccaccaggg cgccgactcc gaggccttct ccttgatcac
     4321 cccgccgaac ttcggatcgg gaggcgggag ctgcttgccg ctgatggtgg tggtggcgcc
     4381 gggcgaaccc agcgtgccgg tgacctgctg cgcgcttgtc gggccggagg ccaacaagac
     4441 ggtgcagacc agaaacagcc ctttggcgat gacgcgattt atttgcatga cccctcctgt
     4501 gcatgaacct gcgttttgtg acggcagtta taccattctt gagccaaatg cggcctgcta
     4561 cccaccttga cgatcaaaaa tatttcatgg gttggcgcac gcctgaacac gatcggcttc
     4621 tgttccccga tcaagcttcc cgcaaggcgt cagcgctcct ggcaccgggt gcggcgtgcc
     4681 ttcctcgtcc atctgtgcgg ccagcgtctc ccattcgacc agcccttggc gatcgagcgt
     4741 caggcgcagc aaccagccgc gtttcgcggc tggtaactcg aacccgtcaa agacaaaatt
     4801 gccaaggctg tagacgatca gtttgccgcg ataatattcg gccccctgtg tgacatgcgg
     4861 atgaccgcca acgaccacat cggcgccggc atcgatcatg ctgcgcgcca gttgcctttg
     4921 ccgttcgctc gggttgggct cgcgctccca gccccaatgc atgaacggaa tcaccagatc
     4981 ggcgcccgca gcgcgcgcgg cacggatgtc gccgatcacc tggctgtctt cgctccaggc
     5041 aattcccggc agatcgggac cggcctcaaa ggagcgcggc ttgaattcgt tgtagccgag
     5101 tacggcaata cgcaaccccc ggcgctcgat ccagagcggt gcatgcgcac cggccagatc
     5161 acgaccgccg ccaaaatagg cgatgtcggc gcggtcgaga tgtccgatcg tttcaaggaa
     5221 tgcctccttg ccgtagtcgc ccgagtgatt gttggcgacc gccagggcgt caaaacgccc
     5281 cttcagcaag ggcacgacgc ggggatcggc gcgaaaggaa acaatcttgc tgtcgagcgg
     5341 tttgccgaca gtggcgatcg ggcattccag attgccgatg gtgaaatcgg catcgcgcag
     5401 cgccgcatcg aaaaaaacca ggggatcgcg ccctgaggcg actacccgcc cgggcccgtc
     5461 gtcgagcatg acatcgccga cgaatatcag gcgcaccggg tcggcctgca cgccggtatt
     5521 gaaaaacagc gccaacagta caaagatcga atggatcggc ttcatcgcac cgtactcgcg
     5581 gcaggccgtc ctgccggggc cgaggattcc ggattcgtca tcgcacacca gtattgcagt
     5641 tcgccatcgc gcaccggttc atacacgaaa tgcaagccac taaagccata gcgtgaatcg
     5701 ctgcttctgg tcggcggata aggataaatc gcctgatgga tcagcacggg cccctcgtca
     5761 aggtcggcat aggtatagat acttatcgac gcaatatccg ccggtttgtc cgtgaacacg
     5821 acccggaaac gaaagtacga gccaatctga atacctttga ttgcataggg tgatgacgcc
     5881 ggatcggcga caagccgttt cgtctcccca ccgtaggtgt aatggcaaac cacctgttca
     5941 gcatgagccg aagtggaggc gatcgccagc aaaccggcca gaacacagcc cgaaaaatat
     6001 ggaacagatt gcccggaaac ccgcactaca acctcccgta tccaacacca agaaactggc
     6061 tacaaggaca gcttcttgaa tatccgctcc ggaatatgcc ggatgatggt catgatcacc
     6121 caccagaaac cgggcagata gacttcgtct gactttcgat cgactgcccg cacgatgccg
     6181 cgcgcgatgt cgtccggctt tgcccaaaga gcgcccttgc tgaacgccgc tgtcatcggc
     6241 gtatcgacga aacccggctt gatcgtcaac acctggattc cctgcttcgc cagccgattg
     6301 cgcaaacctt gcaggaacag actgaccatg cctttggccg cgccataaac ataattgctc
     6361 tgccggccgc gatcaccggc gaccgagccg atcaccgcca ggcttccact gccctgcgct
     6421 gcaaactttt cacccaggag cgtcgccagc gcgataacag acaagccgtt ggtgtcgatt
     6481 tccttgagtg tctgatcgac cgaaacttca catgcctttt gatccggcaa cgagccatgc
     6541 gcgatgagcg cgacatccag cccacccatg gccgacacgg ccgcttcgag catggcagcg
     6601 tgcgccggag tatcgcaagc atccatcgtg tagcagccta ccgaagtcgc gccacgcacg
     6661 cgcagatcgg cagcgatgac gtccagccga tccggctttc gcccgaccag aaacagcgta
     6721 tccctgcgtg ccgcccacag gcgcgccgtt gcttccgcga tggccgaggt ggagccgatg
     6781 atcagaatcc tctgcataat gcttctccgt aatctacgtc acagccgaac cggccaacag
     6841 aacccgccgt gcaaagtcgg atgaaaaccg cggatcgacg aaacgttgaa attcgtcaat
     6901 tcgcggatag ctgcgccgga aaaactcgcc gctcatgcgc gcatccttgg cggcgtagag
     6961 ccgtccaccg gcggcgctca caatggcatc aagacgatca aacaacgcca gcgtggacgc
     7021 gccccgattc gggaaatcga gcgccagggt cactcccggc atggggaagg acaacagacc
     7081 gggactcggc agcgcgccaa acgttttcaa caccgcaagg aaggatccct gcccgctgga
     7141 tgcaatgccg cgcaagacat catcgagtgc cgcgcgctcc gccagcggca acacgaactg
     7201 atactggtag aaccctttct tcccgtagat ccggttccag tgcaggacgc cgtcaagcgg
     7261 atagaagaat ggcgcgtatg acatgcttgt tcgagctgca agcggacgat gatagtaggc
     7321 ttgattgaac gcatggagcg acaaaccatt gattagcgaa aaaggtggcg tcaatggaac
     7381 gctcaagggc ttgccggact cgacgccagg atcgcccgct ttggcatgat cgcccgccat
     7441 gtagatgccg cgcggctttg aggccagaca atcgatccat gccaccgtgt attcgtgcgc
     7501 ggcttcggcg gcagcgttga gcgtaaagaa atcgtcgagc ccgtcaaagc gcgtgttctc
     7561 gacgctgatc atcgggctcg caatccgctt gagcgaaatc gcgacccagg tgatcagacc
     7621 ggtcagcccc aggccgccaa tggttgcggc gaagtagtcg gcgttttccg caggcgaaca
     7681 aacgcgacgc gagccatcgg aacgcagcag ttcgaagcgt tgcacgtagc atccgaaact
     7741 tcctgcccga tgatgattct tgccatgcac gtcgttggcg atagccccgc ccagcgtgac
     7801 aaagcgcgtc cccggcgtca ccggaagaaa ccagccgcgc ggcacgaaca cggcgagaat
     7861 atcggacaac agaatgccgg cctcgcagac cagtgtgccg ctggtgtcat cgaagtcgat
     7921 aaagcggtcc atgccgcgcg tgtgcaacag cgtcgcgcca ctgttcaggc agacatcgcc
     7981 ataactgcgc ccgttgccat gggcgagcag gctggtgtca gtccttggca agtccgcgtc
     8041 acgatccgcc caccaccgct cttcagccgc aggtgtggca ggcagttgcc cccaggcttg
     8101 cagcaccatt atcgctgcct cgccagacac gcggcgccga gcgccacggc aaaccacagg
     8161 gtggccagac ggcaaatcag ggttgctgtc accgccgtca cttcgggcac gccgttgaga
     8221 accaacagcc cgaacatcac cgcttcgctg ctgcccagcc cgccaggcag gaacgagagc
     8281 gccccgacca gcatggcaaa ggcatagatg aatacggccg tcgtcaacgg caggggatgc
     8341 ccgatttcag caagcagcca ccagaagcca aagccctcgg caaaccacgc gagaacgccg
     8401 atgatcagcc ccatgctcat ggccgggagc gaaaagcagc ggcgaaaatg cagaacgatt
     8461 tcgcagagcc ggacgagcaa cttcgcccac ttttgcgggc gcggcatggc ccagcgatcc
     8521 accgccgcaa tccctgctgt ccattgcgcc agcaccagga ccagacacac cgccagcaag
     8581 gccaggccga cgacgggccg tgcgggaacg tagacccaca gaccgatcgc cgcgagcacg
     8641 atgatcgaca gcagatcact cgcacgctcg gcgaagaagg ccgcgaggct ggacgccgcc
     8701 ggtacgccat acggcttgag cagaaccgag cgcagcatct cgcccagttt tcccggactc
     8761 gtggtcaacg caaagcccga gaaataaatc gccagatggt tgcgccaggg cagcgacacg
     8821 tcgagcagcg ccaggtagcg cgaccagcgc acaaagcgca acacgtagtt actcagtgaa
     8881 agcgccagca agccaagcaa aacccacggt ccaacctgaa ccagcgcagc caacacgtcg
     8941 cgccaaccca cccagagcga caacgccaga tagccgacag cggcgaacac cacgctccat
     9001 aacgccgcgc gcagcggcca attcctttcg gcaggcgacg cctgactcga atcagccatc
     9061 gcatcggcac gcggcaagat gcgtcacgct tccctgctta ctgcggcggc gcagaaagac
     9121 tgcctggccg ttgctgcggc gtgcgcaggt tttccctggc agccagatcg ataatgcaga
     9181 ccacgccgtc ctgattggca ctggcattga gaaaggactc caggctattg acgtaactgg
     9241 cctggtcaat tgcaccgcaa tagaaacgcg atgtcagatc catgaagtac gggctgctgt
     9301 cgtacgagga catgttgttg ctgatcatca tgctgccgat gtgtttgccc cagcacttgt
     9361 tctgggcaag cgtcatgaac gcgtcggccg cccctgattg cccttcgcct ggcgcgcagt
     9421 ccgccaactg attgtcgatc ttgtcgaccg cattgcttac aggaccatcc aggggcgatt
     9481 gccatgcgtt gggcgcacat gcgctgagtg ccaatgaaac gattgcagct acagcaaacg
     9541 acggcaatga aaaattattc gtcatgttca gaccttccgg aaaaattcac aaacacaatg
     9601 ccaacggctc ggactagcag atgatgccac cacccaaaca cacacggctc tcatagacta
     9661 cgaccgactg ccccggcgtc atcgcccact ggggttcggc aaactgtatc tcgcacgcgt
     9721 ttgcgtcgat gtgctcgatt tcgcagggtg cgtcgggctg ccgatagcgt gttttggcgc
     9781 agtataccca atgcgttcgc ggcagatcat cgaccccgtc accggctatc catgaaagct
     9841 gttccgcgcg caatacatcg cacagcagcg ccggatggtc gtgcccctgc accacataca
     9901 agatgttctt ttccatgtct tttttggcga caaaccatgc atcgtgctca ccttctttag
     9961 cataggtttt cgtaccgcca atatgcaggc ctttgcgctg gcccagcgtg tgataggtca
    10021 gtccatcgtg ctcaccgaca acacgatcat cgtcgagaca acgaatctcg cctttcttcg
    10081 gcggcagata gcgcatcaga aattccttga acggtcgctc gccaatgaaa cagattcccg
    10141 tcgaatcctt cttctccgcc acatgcagcc ccgctgcttc ggccatcttt cgcacatcgc
    10201 gcttgtagat atccgccagc ggaaacaggg ttttcgaaag ctgggcctga ttcaggcggt
    10261 gcaggaaata actctgatcc ttggtgccat cctcggcctt gagcaactga aatcggccgg
    10321 cgaattcgcg tacgccggcg tagtggccgg tggcaatctt ttcagcgccc atctgcatgg
    10381 catgatccag gaaagcgcga aacttgatct cggagttgca cagcacgtcg ggattgggtg
    10441 tgcggcccgc ctgatactcc ttcagaaact cggcaaaaac gcgctc
//
LOCUS       DAORPM010000062        10470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1443_length_10470_cov_13.141719, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000062 DAORPM010000000
VERSION     DAORPM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1443_length_10470_cov_13.141719"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLD53_04615"
     CDS             complement(<1..998)
                     /locus_tag="PLD53_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HRC60223.1"
                     /translation="MLRIVGATLTALLLTACAPSTLSGGPSAGHLRAESVAQTQGVIP
                     QPVQQIATLPRPRTVEKTETYSVVVNNIKVQDLLFALARDAKLNVDIHPGITGYVSMN
                     AIDQTLPQLLTRISKQVDMRFELDGPNLAVMPDTPYLRNYKIDYVNMSRDVKGTVSTN
                     TQISTSALSTGSGGISGTGNTSRIEIENKSRNRFWESLEKNLKDLLHETDKIFPEGST
                     ETVMEQSASQTTTGTGAQPAAATSRGGQSAQNLANSPNPASLQNSGETIVKRMTFREA
                     ASVVANQEAGVISVRATSRQHEKVQEFIDRVMNSARRQVLIEATIIEVSLSDGYQQG"
     gene            complement(1103..2509)
                     /locus_tag="PLD53_04620"
     CDS             complement(1103..2509)
                     /locus_tag="PLD53_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC60224.1"
                     /translation="MYLDHFGLREAPFRITPHTEFFFAGANRGATLEALIYAITHDEG
                     IVKVSGEVGSGKTMLCRMLLEKLPENVETVYLANPSLSRDEILHAIAEELRAPLLERN
                     SHQLLRALQDRLIDIYATGRQVVVMIDEAHAMPPETLEEVRLLSNLESNRHKLLHIVL
                     FGQPELDDHLGESSMRQLKERITHNFALEPLRRSDIGNYLMFRIRAAGYRGPDLFTSS
                     AIQLISKASEGLTRRVNILADKALLSAFSEDKHQIDNKQVKAAIRDAQFNPMRDGNAK
                     PKTWIAGASVVTVIAVLAYLAGSYKSTNAPSATPAANAPAGTMASANPAAKPPAEVAA
                     TTASTPRNASPEAGISSPASAPSTLHEDQQPTLAERLAASEEWLKTTPNTHYFIQLLS
                     TDGSNQRDVELFLESISGTLDSRQIRVYRSSLSGRDRLGVIYGDYPSREAATAELAKL
                     ARSSLGSNPYVRSINKIR"
     gene            complement(2499..3935)
                     /locus_tag="PLD53_04625"
     CDS             complement(2499..3935)
                     /locus_tag="PLD53_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRC60225.1"
                     /translation="MPLLLIVDDDPLICDTLSYSMSPAFEVITSHSRPRCTQLLRQLR
                     RTPDLALVDLGLPPFPHRPDEGFALISDLLKLCPEIKIVVLSGQSDADNARHARTLGA
                     IDFVAKPCSPADLHKILQRALSYRSLDERRTHGISTSPLIGDSPAIQKLKVQLQQYAD
                     SPFPVLIEGESGSGKEIVASSCLHQDTKRHSRPFLSLNCAAISPALVEPTLFGYAKGA
                     FTGANTAKTGYFEDAADGTLFLDEIGELPLELQAKLLRVLENGEYQRVGETQKCTSRA
                     RIIAATNRDLRKEVRSGNFRADLYHRLSVFSISVPPLREMDDDKFLLLDHFRQRYATQ
                     SQQTAFELSDEARALWSRYSFPGNVRELRNIVIRLTAKHPGQTVSYSALESELDLLDE
                     PLAPVTTTAPVVSAESDALILAASERLQQPNPFRLDRLLAETEQNYIEAAMRLAHGNV
                     SQAARLLGINRTTLYNRMESFAKEQNVS"
     gene            4099..4911
                     /locus_tag="PLD53_04630"
     CDS             4099..4911
                     /locus_tag="PLD53_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC60226.1"
                     /translation="MPADNLVKITDINFAYDDRAILKGINMEIPRGQLVAIMGNSGCG
                     KTTLLRLIGGQLKPTSGELLVAGQSVPDMKHAELYAMRRRMGMLFQFGALFTDISVYD
                     NVAYLMREHTDLPEEMIRDMVLMKLNAVGLRGAHDLMPSELSGGMARRVALARAIALD
                     PMLVMYDEPFAGLDPISMGVIGQLIRKLNDALGATSLLVTHDVHEAMLIVDYVYFVSD
                     GQIVAQGTPDEIRASSDPFVHQFVFGEADGPVHFHYPAVPFEQDLMREGAYG"
     gene            4904..5710
                     /gene="mlaE"
                     /locus_tag="PLD53_04635"
     CDS             4904..5710
                     /gene="mlaE"
                     /locus_tag="PLD53_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HRC60227.1"
                     /translation="MAEKGSPVLLSFVGNIGHRLNSSVQRLGFAARFFVMILVYSGQS
                     FRRLHLTIREMYFSGFQSLLIIMVSGLFIGMVLGMQGYETLQRYGSSEALGVLVALSL
                     TRELGPVVAALLFASRAGSSITAEIGLMKATEQLTAMDMMAVNPIARVVAPRFWGGVI
                     SMPLLAALFSAVGVFGGYLVGVVLIGVDEGAFWSQMQGSVDFRFDVWNGVIKSFVFGV
                     AVSLIAVFEGYDAVPTAEGVSTSIKRTVVYSALAILALDFVLTSFMFRGL"
     gene            5710..6177
                     /gene="mlaD"
                     /locus_tag="PLD53_04640"
     CDS             5710..6177
                     /gene="mlaD"
                     /locus_tag="PLD53_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRC60228.1"
                     /translation="MSRKLLDLWVGFFVVIGFAALLFLALRVGNLSSANFAETYQLTA
                     KFDNIGGLKVRGPVKSAGVVVGRVSEIHFDPQSYEAVATLTIDSRYRFPKDTFVSILT
                     AGLLGEQYIGLDAGGDEKMLKPGDVVVKTQSAVVLEKLISQFMFNKASEGSDK"
     gene            6220..6924
                     /locus_tag="PLD53_04645"
     CDS             6220..6924
                     /locus_tag="PLD53_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRC60229.1"
                     /translation="MCSVAFVTAILGGLTGCATTADNPQDPYEGFNRAMFAVNEGIDV
                     VVKPVAQGYEAAAPLPVKAGIGNFFGNLWDVITALNGLLQGKGEQGLSDIGRVLINTT
                     VGIGGVFDVASEMGLEKHSEDFGQTLGVWGVGEGPYFYWPLIGPRTTRDTFGWMVDSY
                     MDPVWNISDVPVRNSLVGVRYIDLRASLLPTDKIVDQAAFDKYDYIREAYLQNRRSEV
                     NDGHEDKSECKQSRCY"
     gene            6964..7578
                     /locus_tag="PLD53_04650"
     CDS             6964..7578
                     /locus_tag="PLD53_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC60230.1"
                     /translation="MKSFIAVVLGLVVTCSVAFAQEDPPDALIKKVTEDVLTIVRQDK
                     DIQNGNSKKAIELVEAKVLPNFNFQRMTALAVGRDWNKATPEQKKRLSEAFRTLLVRT
                     YSNALTGYKDQTVRFKPTKMQSGDTDVEVRTEIVQPGSKPIQLDYSMEKQADGWKVYD
                     VVVAGVSLVSNYRNTFSQEVRANGVDGLVRMIENKNKQLEASNK"
     gene            7575..7868
                     /locus_tag="PLD53_04655"
     CDS             7575..7868
                     /locus_tag="PLD53_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRC60231.1"
                     /translation="MIEQNGNELRITVPMLIANASALLEAGRDFLRGGGGAGEVVFDL
                     SAVTETDSSALSVVFGWLRTARERSVSVRVAHPPASMISQAELYGVSDSLPLA"
     gene            7905..8822
                     /locus_tag="PLD53_04660"
     CDS             7905..8822
                     /locus_tag="PLD53_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC60232.1"
                     /translation="MGVAVSIKQIVKSFGHLRALDDVSLEIAEGEFFGLLGPNGAGKT
                     TLISCLAGLVRPDRGQLSVMGCDVLGDYREARRRLGVVPQELVFDPFFSVRETLRIQS
                     GYFGIRNNDDWIDEILANLDLTAKAATNMRRLSGGMKRRVLVAQALVHKPPVIVLDEP
                     TAGVDVELRQGLWQFVQRLNREGHTVILTTHYLEEAEALCGRIAMLKQGRVVALDSTS
                     NLLARYAGHTLKLRLSRCDGLPEDLRARSTADQEHHVFRLAGFDEIELLLGRIREAGC
                     TIEDLRLTQTDLEDVFVGVMRGTDAGRES"
     gene            8827..9579
                     /locus_tag="PLD53_04665"
     CDS             8827..9579
                     /locus_tag="PLD53_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60233.1"
                     /translation="MTGFQTLLYKEVLRFWKVSFQTVAAPVLTALLYLLIFSHVLDAR
                     LLVNGVRYAAFLIPGLVMMSILQNAFANSSSSLIQSKVTGSIVFVLLPPISYVEFFFA
                     YVVAAMLRGLMVGIGVLLVTGWFASLSFVAPIWILAFALIGGGIMGSLGMIAGIWADK
                     FDQLAGFQNFLILPLTMLSGVFYSIHSLPLFWQQVSHFNPVFYMIDGFRFGFFGVSDV
                     SPEMSLLVVTGSFIVVALITLGLLRSGYKLRN"
     gene            9583..9828
                     /locus_tag="PLD53_04670"
     CDS             9583..9828
                     /locus_tag="PLD53_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRC60234.1"
                     /translation="MMHSEQIKKIIAEGLPCELLNVEGDGDHFEALIVSAEFIGKSRV
                     QRQQLVNALLRSHFDSGKLHALSMKTVTPEEWSALRG"
     gene            9821..>10470
                     /locus_tag="PLD53_04675"
     CDS             9821..>10470
                     /locus_tag="PLD53_04675"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRC60235.1"
                     /translation="MDKLLIQGGSPLHGEVSISGAKNAALPILCASLLSAEPLHITNL
                     PHLNDVSTMLRLIGQMGVEVTMDGNDGLILNSHGLNNPAAPYDMVKTMRASILVLGPL
                     LARCGEAKVSLPGGCAIGARPVDQHIKGLQAMGAEIHVENGYVLAKARRLKGARIVTD
                     MVTVTGTENLMMAAVLAEGETVIENAAREPEVLDLANCLISMGAHISGAGTDSIKIR"
BASE COUNT         2015 a         2645 c         3249 g         2561 t
ORIGIN      
        1 ccctgctggt aaccatcgct aagggaaact tcgatgatgg tggcctcaat cagcacttgg
       61 cggcgggcac tgttcattac ccggtcgatg aactcctgta ccttttcgtg ttgccgcgat
      121 gtggcgcgca cgctaattac tcctgcttcc tggttggcga cgacggaagc cgcttcgcgg
      181 aaggtcatgc gcttgacaat cgtctcgccg ctgttctgca ggctggccgg attcgggctg
      241 ttggcgaggt tttgcgcgga ctggccgccg cgggaggtgg cggcggcggg ttgtgcgccc
      301 gtgccggtgg tggtttgcga agcgctctgc tccatcacgg tttcggtcga gccctccggg
      361 aatatcttgt cggtttcgtg cagcaagtcc ttcagatttt tctccagcga ttcccagaaa
      421 cggttcctgg acttattctc gatctcgatg cgcgaggtgt tgccggtgcc agaaatccct
      481 ccgctaccag tagacagtgc gctggtcgaa atctgggtat tggtcgatac cgttcccttt
      541 acgtcgcgcg acatgttgac gtaatcgatc ttgtagttcc tgaggtacgg cgtgtcgggc
      601 atcacagcga gattggggcc gtcaagttcg aagcgcatgt caacctgctt ggaaatgcgg
      661 gtcagaagtt gcggcagcgt ctggtcgatg gcgttcatgc tgacatagcc ggtgattccc
      721 ggatgaatgt cgacgttcag cttggcatcg cgagcaagag cgaaaagcag atcctgaacc
      781 ttgatgttat tgaccacgac gctataggtt tcagtcttct cgaccgttct gggtctgggg
      841 agggtggcaa tctgttgaac gggctgcgga atcacgccct gcgtttgcgc gacgctttct
      901 gcgcgcagat gcccggctga aggccccccg ctcagcgtcg aaggggcgca ggcggtgagt
      961 agaagcgcgg tcagggtggc gccgacaatt ctcagcatgc ttggtctcat tcccgatgga
     1021 ttgaaattat catatcacgt aaggtgtttt cttcaagtga ctgattttta ttgaatgagt
     1081 gccgaggttt cttgtgtcgc tttcagcgta ttttgttgat cgagcgaaca taggggttgc
     1141 tgcccaggct tgacctcgca agcttggcca attccgcggt ggcagcttcg cgcgaggggt
     1201 aatctccgta gataaccccc agccgatcac gtccgctgag gctggaacga tagacgcgga
     1261 tttgccggct atcaagcgtg ccggaaatgc tttccagaaa cagttcgacg tcgcgctgat
     1321 tgcttccgtc ggtactcaac aactggatga agtagtgcgt gttcggggtt gtttttagcc
     1381 attcctcact tgccgccagg cgctcggcaa gtgttggctg ctggtcttca tgcagggtgg
     1441 agggagcgga tgcagggctg gagatgcctg cttctggtga agcattcctt ggcgtcgatg
     1501 cggtagttgc agcaacctcg gccggcggtt tcgcagctgg atttgcagat gccatggtgc
     1561 ccgcaggtgc gtttgctgca ggggtggccg acggtgcgtt ggtcgatttg tagctcccgg
     1621 cgaggtaggc taaaaccgca atgactgtga cgacgctggc gccggctatc catgttttgg
     1681 gcttggcgtt accgtcacgc atcgggttga actgggcatc gcggatcgcc gctttgacct
     1741 gcttattgtc aatttgatgc ttgtcctcgg aaaaagccga gagcagtgcc ttgtcggcga
     1801 ggatattcac gcgccgcgtc agtccctccg acgctttgct gataagttgt atggcgctcg
     1861 aagtaaaaag atccggtccg cgataaccag ctgcacgtat gcggaacatc aggtagttgc
     1921 cgatgtcgct gcggcgtagc ggctccagcg cgaagttgtg cgtaattcgc tccttgagct
     1981 gccgcatgct ggattcgccc agatgatcat ccagttctgg ttggccgaag agcacgatgt
     2041 gcagaagttt gtgccggttg gactcaaggt tggacagcag gcggacttct tccaatgtct
     2101 cgggaggcat ggcgtgcgcc tcgtctatca tgacaacgac ctgacggccg gtcgcgtaga
     2161 tgtcaatcag acgatcctgc agagcgcgca gcaattggtg agagttacgc tccagcagag
     2221 gtgcacgcaa ttcctccgca atggcgtgaa gaatttcatc acgcgacagc gaggggttgg
     2281 ccaggtagac cgtctcgacg ttctccggca gcttctccag cagcatccgg cagagcatgg
     2341 tttttccact gcccacttca ccgctgacct tgacgatgcc ttcgtcgtgc gtgatggcgt
     2401 aaatcagtgc ttcgagcgtg gctccgcgat tggcgcccgc aaaaaagaac tcggtatgcg
     2461 gtgtgatgcg aaacggggct tcgcggagtc cgaaatggtc aagatacatt ctgttccttg
     2521 gcaaaggact ccatacggtt gtagagcgta gtgcgattga tgccgagcag acgcgccgcc
     2581 tggctgacgt tgccgtgcgc gagtcgcatg gcggcttcga tgtagttctg ctcggtttcg
     2641 gcgagcagcc gatcaagtct gaacgggttc ggttgctgca gacgctcgct ggctgccagg
     2701 ataagcgcat cactttctgc gctgacgacc ggcgctgtag tagtgactgg cgcaagcggt
     2761 tcgtcgagca aatccagttc actctcaagg gcgctgtaac ttacggtttg tccagggtgc
     2821 ttggccgtga ggcgaatgac aatgttgcga agttcccgca cgttgccggg aaaactgtag
     2881 cgcgaccaga gtgcgcgtgc ctcatcggac agctcgaaag ccgtttgttg agattgcgtt
     2941 gcgtagcgct ggcgaaaatg atcgagcaag aggaacttgt cgtcatccat ctcacgcagg
     3001 ggtggcacgc tgatcgagaa cacggaaagc cgatgataaa gatcggcacg gaagttgccc
     3061 gaccggacct ccttgcgcag gtcccggttg gtcgcggcaa tgattcgtgc acggctggta
     3121 cacttctgtg tttcgccaac ccgctgatac tcgccattct caagcacgcg taacaatttt
     3181 gcttgcagct ccagcggcag ctcaccgatt tcgtcgagga aaagcgtgcc atcggcagcg
     3241 tcttcgaaat aaccggtttt tgccgtgttg gcgcctgtga acgcgccctt ggcatatccg
     3301 aacagggtgg gttcgaccag agcaggggaa atggccgcgc agttgagcga tagaaaaggc
     3361 ctgctgtggc gcttggtgtc ctgatgcagg caactgctgg caacgatttc cttgccgctt
     3421 cccgactcgc cctcgatgag cacgggaaaa ggcgaatcgg cgtattgctg caactgcact
     3481 ttcagtttct gtatggccgg gctgtcgccg atcaggggcg atgtggaaat gccgtgggta
     3541 cgccgttcgt ccagtgagcg atagcttaac gcccgctgga gaatcttgtg caggtcggcg
     3601 gggctgcaag gttttgcaac aaaatcgatg gcgcccaggg tgcgggcgtg acgggcattg
     3661 tccgcatcgc tttggccgga gagtacgacg atcttgattt ccggacacag tttcagcaaa
     3721 tccgaaatca aggcgaatcc ttcgtccggg cgatgcggga agggaggcag gccgaggtcg
     3781 accagcgcca gatcaggagt gcggcgaagt tggcggagca attgagtgca gcgcgggcgg
     3841 gagtggctgg tgatgacttc aaacgcgggg ctcatcgaat agctcagcgt gtcgcatatt
     3901 agcggatcat catcgacgat gagcagcagc ggcatttttg gcaaggaagt ttccggggcg
     3961 ttgctcatgg aaagcgcgtc atcggtctgg aaatgaacaa ttccgttatt ctacaattgt
     4021 ttggctgaga atcttttgcg gatggcgaac cgtcattcga gtataatcaa cccccatttt
     4081 tcttcttcgg actccgccgt gccagccgac aatctggtca agatcactga catcaatttc
     4141 gcctatgacg atagggccat cctcaaggga atcaatatgg agattccccg aggccagctg
     4201 gttgccatca tgggcaactc aggctgcggc aagacgacct tgttgcggtt gattggcggg
     4261 cagctcaagc caaccagcgg cgaattgctg gtcgctggcc agtcggtgcc ggacatgaaa
     4321 catgcagaac tttatgccat gcgacgacgc atggggatgc tgttccagtt tggcgccttg
     4381 ttcaccgata tttctgtgta tgacaatgtc gcctatctga tgcgtgagca taccgacctg
     4441 ccggaagaga tgattcgtga catggtgttg atgaagctca atgccgttgg cttgcgggga
     4501 gctcatgacc tgatgccgtc cgaactgtcg ggcggcatgg cgcgtcgcgt tgcactggcg
     4561 agggcaatcg cgctggatcc gatgctggtc atgtacgacg aaccctttgc cgggctggat
     4621 ccgatctcga tgggtgttat cggtcagctc atccgcaagc ttaacgatgc actcggcgcg
     4681 acgtcgctcc tggtgactca cgatgttcat gaggcgatgt tgatcgtcga ttacgtctat
     4741 tttgtctccg acgggcaaat cgttgcgcag ggaacgccgg acgagatacg cgcgtcctcc
     4801 gatccttttg tgcatcagtt tgtttttgga gaggccgacg gccccgtgca tttccattac
     4861 ccagcggttc ccttcgagca ggatttgatg cgagaaggcg cttatggctg aaaaaggttc
     4921 tcctgtcttg ctttcgttcg tcgggaatat cggccatcgc ttgaacagca gcgtgcagcg
     4981 gttgggcttt gccgcacggt ttttcgtgat gatcctggtc tattccggac agtcattcag
     5041 gcgtcttcac ctgacgattc gtgaaatgta cttttccggt tttcaatcct tgctgatcat
     5101 tatggtttca ggactgttta tcggcatggt gctgggcatg caagggtatg agactttgca
     5161 acgctatgga tcttccgaag cgcttggcgt gctggtggcg ctttcgctga cgcgtgagtt
     5221 ggggccggtg gttgctgcat tgctgtttgc atcgcgcgcg gggtcgtcga ttacggccga
     5281 gatcggcttg atgaaggcca ctgagcaact gactgcgatg gacatgatgg cggtgaatcc
     5341 gattgcccgt gtcgtggcac cgcggttctg gggcggggtt atctccatgc ccttgctggc
     5401 cgccttgttt tccgcggtcg gtgtgttcgg cgggtatctg gttggtgtcg tcctgatcgg
     5461 tgtcgacgaa ggcgctttct ggtcgcagat gcaagggtcg gtggacttcc gcttcgacgt
     5521 ctggaatggc gttatcaaga gttttgtttt tggggtggct gtttccctga ttgctgtttt
     5581 cgagggctac gatgcagtgc cgactgccga gggcgtttcg acttcgatca aaagaacagt
     5641 tgtgtattcg gcgctggcga ttctggcgct ggatttcgtt ttgacctcct tcatgttccg
     5701 ggggctttga tgagtcgtaa gttacttgat ctatgggttg gattctttgt cgtcatcggt
     5761 ttcgcggcgt tgttgttttt agcactaaga gtcgggaacc tttcgtcggc caattttgcc
     5821 gagacttacc agttgacagc caagtttgat aacatcggtg gcctgaaggt gcgtggtccg
     5881 gtaaagagtg cgggggttgt tgtcgggcgg gtttcggaga ttcacttcga tccccagagc
     5941 tatgaggcag tggcgacgct tacgattgac agccgttacc gttttccgaa agacactttc
     6001 gtttcgattc tgacagcggg tctgctgggc gagcaataca tcggcctgga cgctggaggt
     6061 gacgaaaaga tgttgaaacc gggcgatgtg gtcgtcaaga cgcaatctgc agttgtgctt
     6121 gagaaactga tcagtcagtt catgttcaac aaggcgtccg aaggttcgga caagtaataa
     6181 ctggaagggt cgtccgtgaa aaaactaata gcaaagcgca tgtgttctgt ggcattcgtc
     6241 actgcgatcc tgggcgggct gacaggctgc gcgacgacag cggacaatcc acaggatccc
     6301 tacgagggtt tcaaccgggc aatgtttgcg gtgaatgagg ggatcgatgt agtggtcaag
     6361 ccggtcgccc aaggctatga agccgccgcg ccgttgcccg tgaaggccgg tattggtaat
     6421 tttttcggta atttatggga cgtgataaca gcgcttaacg gcttgcttca aggcaagggt
     6481 gagcaggggc tgtcggatat tggccgtgtg ctgatcaata ctacagtcgg cattggcggc
     6541 gttttcgatg tggccagcga aatggggctg gagaagcatt ccgaagattt tggccaaacg
     6601 cttggtgtat ggggtgttgg cgaggggccg tatttttatt ggccgctgat cgggccgcgc
     6661 acgacgcgtg acacattcgg atggatggtc gactcttata tggacccggt ctggaatatt
     6721 agtgatgtcc ccgtgcgtaa ttcgctggtc ggcgtgcgct atattgatct tcgtgcgagc
     6781 ttgctgccca ctgacaagat tgttgatcag gcggcgtttg acaagtacga ctacattcgc
     6841 gaagcttatc tgcagaatcg gcgcagtgag gtcaatgacg gccacgaaga caagtctgaa
     6901 tgcaagcaaa gtcgctgcta ttgattattg cggttggtca gaccggtttt ttgagagaag
     6961 ttgatgaaat cgtttattgc agtagttttg gggctggtgg tgacgtgttc ggtggcattc
     7021 gcgcaggaag acccaccaga tgccttgatc aagaaggtga ctgaagacgt tttgaccatc
     7081 gtccgccagg ataaggatat tcagaacggc aattcgaaga aggccattga actggttgag
     7141 gccaaggttc tgcccaactt caatttccag cgcatgacgg cgctggcagt ggggcgcgac
     7201 tggaacaagg cgacacccga gcagaagaaa cgtttgtccg aggcgttcag aacgcttctt
     7261 gtgcgcacct attccaatgc cttgaccggt tacaaggatc agaccgtacg cttcaagccg
     7321 accaaaatgc aaagcggtga tacggatgta gaggtcagga ccgagattgt gcagcctggc
     7381 agcaagccca tacagcttga ctattcaatg gaaaagcagg cggatgggtg gaaggtctac
     7441 gatgttgtcg tggccggtgt aagtttggtg tcgaactatc gcaatacctt cagccaggaa
     7501 gttcgcgcga acggtgtcga cggccttgtc cggatgattg agaacaagaa caagcagctt
     7561 gaagccagca acaaatgatt gaacaaaacg gtaatgagct gcgtataacg gttcccatgc
     7621 tgattgccaa cgccagcgcc ctgctcgaag cagggcgtga ttttttgcgc ggcggaggtg
     7681 gagcgggtga ggtcgtgttt gacctttcag cggttaccga aaccgactct tcggcgctga
     7741 gtgtcgtgtt tggctggctg cgaaccgccc gtgagcgcag cgtttccgtg cgcgtggccc
     7801 atccgccggc aagcatgatt agccaggccg agctttacgg ggtttccgat tctcttccgc
     7861 tcgcctgatc ggcgcttgcg cccttgctgt tgagttcaga tgtcatgggc gtcgccgttt
     7921 ccatcaagca aatcgtaaaa agttttggcc acctgcgagc ccttgatgac gtttcgttgg
     7981 aaattgccga aggcgaattc tttggtctct tgggacccaa cggcgccggc aagacgacgc
     8041 tgatttcctg ccttgccggt ttggttcgcc cggatcgagg ccaattgagc gtgatgggtt
     8101 gcgatgtgct tggcgactac cgcgaggcgc gtcgtcgact cggtgtggtg ccacaggaac
     8161 tggttttcga tccctttttt tccgtgcgcg agacattgcg cattcagtcg ggctacttcg
     8221 gaattcgcaa caacgacgac tggatagacg agattcttgc gaatctagac cttaccgcca
     8281 aggccgctac caatatgcga cgactttcgg gcggcatgaa gcggcgggtg ctcgtcgcgc
     8341 aggcgctcgt tcacaagccc ccggtaatcg tactcgacga gccgacggca ggcgttgacg
     8401 tcgaactgcg ccaggggctg tggcaattcg tgcagcgact caatcgcgaa gggcatacgg
     8461 tcattttgac cacacattat cttgaagagg ccgaggcgtt gtgtgggcgc attgccatgc
     8521 tcaagcaggg gcgagtcgtt gcacttgatt cgacgagtaa cttgctcgcg cgctacgcgg
     8581 ggcacaccct gaagctgcgt ctgagccgtt gcgacgggct gccggaagac ctgcgcgcgc
     8641 ggtcaacagc cgatcaagaa caccacgtct ttcggctggc cggctttgac gaaatcgagc
     8701 tcttgctggg gcgtattcgc gaagcgggtt gcacgattga agatctgcgc ctgacacaga
     8761 cggacctgga agacgtcttt gtcggcgtaa tgcggggaac cgatgccggg cgggagtcct
     8821 gacgcaatga ccggtttcca aaccctgctc tacaaggaag tgctgcgttt ctggaaggtg
     8881 agctttcaga cagttgccgc gcccgtcctg acggcgctgc tatacctgct gatcttctct
     8941 catgttctgg atgcacgcct gctggtcaat ggcgtgcgct acgccgcctt tcttattccc
     9001 ggactggtca tgatgtcgat tctgcagaat gcctttgcca acagctcttc aagcctgatc
     9061 cagtccaagg ttactggcag tatcgtgttc gtgctgctgc cacccatttc ctatgtcgaa
     9121 ttctttttcg cgtatgttgt tgccgccatg ttgcgtggtc tgatggtagg gattggagtg
     9181 cttctggtga cgggatggtt cgcatcgctg agtttcgtgg cgcccatctg gattctggcc
     9241 ttcgccctga tcggcggcgg tatcatgggg tcgctgggca tgatcgcggg catctgggct
     9301 gataagtttg accagcttgc cggttttcag aattttctga tcctgccttt gaccatgctt
     9361 tccggcgttt tctattcgat ccattctcta cccttgttct ggcagcaggt ttcgcatttc
     9421 aatccggtgt tctacatgat cgacggtttt cgtttcggtt tctttggtgt ttccgacgtg
     9481 tcgcccgaaa tgagtctgtt ggttgttacc gggagtttta ttgtcgttgc actgatcacg
     9541 ctgggcctgt tgcgcagcgg ctacaagttg aggaattgaa ccatgatgca ctccgaacaa
     9601 atcaagaaaa tcatcgccga aggcttgccg tgcgaactgt tgaatgtcga gggcgacggc
     9661 gaccacttcg aggcgctcat cgtcagtgca gaattcatcg gcaagagccg ggtgcagcgt
     9721 cagcaattgg tcaatgctct gctgcgttcg cattttgata gtggcaagct gcatgctctt
     9781 tcgatgaaga ccgtgacacc ggaagagtgg agcgcgctgc gtggataaat tgctgattca
     9841 gggtggttca cccttgcacg gagaggtatc gatttccggc gccaagaatg ccgcactgcc
     9901 gatactctgc gccagcctgc tttccgcaga gcctctgcat ataaccaact tgccgcatct
     9961 caacgatgta tcgaccatgt tgcgcctgat cgggcagatg ggcgtcgagg tgacgatgga
    10021 tgggaacgat ggcctgattc tgaatagtca tggcctgaac aatccggccg caccgtacga
    10081 tatggtcaaa accatgcggg cctcgattct cgtcctcggc cccttgctgg cgcgctgtgg
    10141 cgaggccaag gtttcgctgc cgggtggctg cgccatcggt gcccggccag tcgatcagca
    10201 catcaagggg ttgcaggcga tgggtgccga gatccacgtc gagaacggtt atgtccttgc
    10261 caaggcgagg cgcctgaagg gagcgcgcat cgtgaccgat atggtgacgg tgaccggcac
    10321 cgaaaacctg atgatggcgg ccgtattggc tgaaggggaa acagtcatcg aaaacgccgc
    10381 gcgcgaacct gaggtgctcg atctggctaa ttgcctgatc tcgatgggtg ctcacatttc
    10441 aggtgcggga accgacagca tcaagattcg
//
LOCUS       DAORPM010000063        10470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1444_length_10470_cov_11.413922, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000063 DAORPM010000000
VERSION     DAORPM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1444_length_10470_cov_11.413922"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1731
                     /locus_tag="PLD53_04680"
     CDS             286..1731
                     /locus_tag="PLD53_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60236.1"
                     /translation="MSFRGLNTLLLLVTVLAGCAGNPMRQYDDELKETVKLVNNGSVK
                     QAINLLEKNNEPGVITKDKDILYYFENGELLTLDNNYAAGRDSWLKADEYVRQWEDAV
                     NTDAAQMLGSVGSYLVNDKTRRYEGQDYEKVMLSTNLTLNHIMLGNYDLARIEMKKTY
                     EREKLIESFREKEYDALQEDAKTKNVTVDVTQMKDYPMAELDTPEVNSLKNGFQNAFA
                     HYLAGYFFEVTGEPSLSEPGYRNALKLAPNSRIVQSGLKGVGRHRPGAKESDVLFVIE
                     SGFAPAWKSITIPIPVPRQTGFMVTPLSFPVIKSENKGFVPSTVSVAGKQLSVETLTN
                     IDTMARRQLKDQMPAILLRTVIRAVVKSVAQDQAYKGGVVLGVLTNVATVATEQADER
                     SWRTLPDRIAVARATLPQGNQTIEFQTGTGTFRKEVAIGSRFTIIPIRITSGTVYMGQ
                     PNTLGSGLSELSADTAVSKKASKKTVKKTNQ"
     gene            1767..3215
                     /locus_tag="PLD53_04685"
     CDS             1767..3215
                     /locus_tag="PLD53_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HRC60237.1"
                     /translation="MKRSILFGLSVLISLLLGSSVMAATERRVALVIGNNDYQSIVKL
                     EKAVNDANAVAAELRKVGFEVEVLNNVGRKKMNLAINDFVQKVSGGGVGVFFFAGHGL
                     QINNQNYLVPIDMDTPNDPADVVDQAVSVPILQDKLADAKAKYTLLVLDACRNNPLPK
                     KAGRSIGTTRGLAMTNSPNGQTVLYSAGANQEALDSLGNNDKNPNGLFTREFLPTISQ
                     PGISSTEALKKTRSMVIQKAKSVGHEQQPAIYDQADGDFYFVSGPAPAQRSTAAGAAG
                     STTTIVQAVDPQAIELSFWDTIKDSKQSADYQDYLAKYPNGQFAPLASRRVAALSQTV
                     SRSSDAVPASASAPSAPSAVASEPTQVAMGSAPPPPAAAPSASSGPSMASKIEEQGKM
                     NNLKVTDMRATKRDNLLRIQVDVTNISSSNQQLYYRFKWLDRDGFTVWDEEPWKPMIV
                     YGNQKQVINVVSPTFKATDFRLILQSPDNSGN"
     gene            3239..3649
                     /locus_tag="PLD53_04690"
     CDS             3239..3649
                     /locus_tag="PLD53_04690"
                     /inference="COORDINATES: protein motif:HMM:NF018889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HRC60238.1"
                     /translation="MKALFGPFGRFTLVAASVLALAPMLASGGSASQTSRPVSHGYAA
                     LTQDASGDIAISDLRATMQDRLLRVQADLRNPTTSDRQLYYRFEWLDQDGLAVWDDEP
                     WKPMIVYGQSKRTISVSSPSFKATRFRLVVQNPN"
     gene            3716..4333
                     /gene="lpoB"
                     /locus_tag="PLD53_04695"
     CDS             3716..4333
                     /gene="lpoB"
                     /locus_tag="PLD53_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HRC60239.1"
                     /translation="MYSKPNFDRPATLLVGTLMLVLAGCQTTSPTTGTSGVSYGDSKA
                     VETVTTDLGSTDIQMISEKMTQSLIQTPEIQTLIKKRQLLMASPVKNKTSEYFDTRLI
                     TDTVLTQLQKNGVRYAIEGEDMQNQTDELRRQSQSGLYDKTKSVKVGKMQGAKFRLDG
                     SISSIIKKTSDIKDVYYKMNLRLIEIETGIVEWSDEKEIRKSARR"
     gene            4370..5203
                     /locus_tag="PLD53_04700"
     CDS             4370..5203
                     /locus_tag="PLD53_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60240.1"
                     /translation="MNSKWFTRVGFTLFATFSIAASAATPKVAVTDLAYEERVSEYFR
                     VVSASQKSSLRASGRESERESDYAYSRRSSGSVNARSESSYSSTEGTYSYIDRGEMRK
                     FTADIKGGILKTGRFQLVQGRPFTDAKNNEKLYDIIGRIKKGMYPGADYVLFGTINSI
                     EFRQEANPIDHTNTFSHTLSLELVGEFSLISTRNFTVKSSFSAMGAGQDVKMLTSQGG
                     RVVLNRGKVVSEVSKSLGEDVARQLEEQLSGVYGGNEESGEKYPSSRQESPREEVIIF
                     R"
     gene            complement(5244..5891)
                     /locus_tag="PLD53_04705"
     CDS             complement(5244..5891)
                     /locus_tag="PLD53_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60241.1"
                     /translation="MHKAYWLGSVLLVLLSMGASAAEVALVTAASGNVKLQEEKAAAS
                     ELKPFIKVREGDRLLMEGASRLQVVYFEGGRQETWSGRGALEVGSVSSKAIKGTMQPE
                     IKTLPAILVKQLAKTPAQDGNVKTGMIRMRSMPPYDRLETVEKNYDEMRKQAVAGDLN
                     PELYLLASYFELREFDKLEALLRQLNEKASETQELAALNMLYARAIRDAKSTAKQ"
     gene            complement(5884..7812)
                     /locus_tag="PLD53_04710"
     CDS             complement(5884..7812)
                     /locus_tag="PLD53_04710"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC60242.1"
                     /translation="MLGRPRKSRRLWLVFFALAACLIAEALLRGGVLNPVDQRLQDFW
                     FQWQGVRAEARHVGIVALDEDTLAAYPDDPMIFWTDRLAVAVARLREVGANVVGLDML
                     LSISPERWLGKLGGDLQQAARDYDRTFREQINSGQLVLASTRSGSGGRETDYLLPSPD
                     YLLALPDFDIPGYVALADLLDEGDGVIRRYLMAPVALADRILLDGNVPILGLPSLLAV
                     RAAGLDPLATSWMIGGRAMDREQPPEPIPYFGPPGTFPRVSLTRLLSDDALNDPAVTA
                     LQGKTVLIGATAAGLNDEHFTPYATRLFSGRGVLMSGVELHANVLESLLSGERLQPLG
                     DGPRIGTLLLMVGLAVVAFVALPVWAGALLWLATIPVLALSGFLAFRFDVLAPVSAYA
                     VATAIALLGVLGWRLTGEERERSRVRQMFGRYVSDQVVEALLKSDTRPELGGQSQEIT
                     VLFSDIRNFTTISERLNAREVVEMLNTYFERACAPLLAEGGSIDKFIGDAIMVEFGSP
                     LPLADHANRGIRAAIALRAVAEEFSVWMEKRFPDRNLPKFAVGIGLHSGEAVIGNIGS
                     LKRMEFTAIGDTVNLASRLEGMTKEIGCAILASETTIVAAGRNVVCGRSEIVKVKGRE
                     EAVRVFEVLNVEGGEKDA"
     gene            complement(7815..8681)
                     /locus_tag="PLD53_04715"
     CDS             complement(7815..8681)
                     /locus_tag="PLD53_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRC60243.1"
                     /translation="MIFSMTGYASRTLDVDCGALHIELKSVNSRYLDFQFRLSDELRI
                     VEPALRELFASRLTRGKLECRLNFVSATAHAQSLVLNVELLQRLKSFDDRIRSELVNA
                     APLAVSDVLRWPGMFAEDALDIEALLPDCLIVAKDVLEDFSASRAREGEKLAAVILER
                     VARMRELVREVAPRIPAAQAAFQEKLKQRLFDAIHSVDDERIRQEVAVFAVRIDVAEE
                     LARLSTHLDEVERVLKAGGACGKRLDFLMQELNREANTLGSKSVVSEVSQTAMELKLL
                     IEQMREQIQNLE"
     gene            8782..9732
                     /locus_tag="PLD53_04720"
     CDS             8782..9732
                     /locus_tag="PLD53_04720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRC60244.1"
                     /translation="MAQQANHALPSGFQLDVYHIEHQLSLGGFSIVYLATDPEGKFVA
                     IKEYLPNSLALRGEGGIKPVISDEHMPAFRYGMKCFFEEGRALAKLSHPNVIRVLNFF
                     RGNDTVYMVMEYEQGRTLQEFIQKNRAVVTENFIRNVFTKLLNGLREVHAHKLLHLDI
                     KPSNVYMRNDYTPVLIDFGAARQTLASDTPMLKPMYTPGFASPEHYKQRELLGPWSDI
                     YSVGASMYASLAATAPQSADSRQEKDKVIPAMIRWQGQYSEQLLKTVDWCLSLDHLSR
                     PQSVFALQKALTKKVPKPELRKKHWLDHVVGKFKRKIEVQ"
     gene            9729..>10470
                     /locus_tag="PLD53_04725"
     CDS             9729..>10470
                     /locus_tag="PLD53_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HRC60245.1"
                     /translation="MKFTIYQESRPGKRANNEDRLAHCYSRDALLLVIADGMGGHYYG
                     EVAAQIAVQVLVETFQRDAKPEIRDPFMFLHKAMTTAHRAIVDFTDAHKLKDSPRTTC
                     VACIIQDNIAYWAHAGDSRLYLLRGGKVVTHTRDHSRVQLLIDQGLINESQAAVHPDR
                     NKVYSCLGGQQSPEIEFSRKTPLVAGDVLALCTDGVWGVIPDDTLAVALKEKNLMQAV
                     PMLLGQADIRGGPNADNLSIVVVRWEDSY"
BASE COUNT         2512 a         2918 c         2872 g         2168 t
ORIGIN      
        1 cgtatgagtg acaggttttc gggacgcgac gaagccatta cgcggtaagg aatgcaccgg
       61 tattttctgt gaaacacgcg ggcatatggc attgccgggg atgaagggtt actattcccc
      121 gaaatgaaag gcctgtcgaa aggcctgtgc gtctcggtgc ctacttgctg tagtgtcgaa
      181 catcgagagt aagctggttt caaaaaattt gcgtatggcg gttgaatcac cggcaatcat
      241 tgcacggtgt cgtcgcctca atcgattcat cgggggatga aaaatatgag tttcagggga
      301 cttaacacac tgttgttgct tgtaaccgtt ctggctggtt gtgctggaaa cccgatgcgt
      361 cagtacgatg atgagttgaa ggaaaccgta aagctggtca ataacggctc ggtcaagcag
      421 gctatcaatc tactggaaaa gaacaatgag ccaggcgtta taaccaagga caaggacatt
      481 ctctactatt ttgagaatgg cgagttgctg accctggaca acaactatgc ggcgggtcgg
      541 gattcgtggc tgaaagccga cgagtatgtt cgccagtggg aagacgccgt caatactgat
      601 gcagcccaaa tgctgggaag tgtcggaagc tatctggtca atgacaagac gaggcgttac
      661 gaaggtcagg attacgaaaa ggtcatgctg tccaccaatc tgaccttgaa tcacatcatg
      721 ctgggcaact atgatctggc ccggatcgag atgaagaaaa cttatgagcg ggagaagttg
      781 atcgaaagct tccgcgaaaa ggaatacgac gcgctgcagg aagacgccaa aacgaagaac
      841 gtcacggtcg atgtcacgca gatgaaggat tacccgatgg ctgagctgga cacgccggag
      901 gtgaatagtc tcaagaatgg tttccagaat gctttcgctc actatctggc cggttacttt
      961 tttgaggtaa ccggcgaacc gtctctgtcc gagccgggtt atcgcaacgc cctcaagctg
     1021 gcgccgaatt cccgcatagt tcagtcgggg ttgaagggag ttggccgcca ccggccaggt
     1081 gcgaaagaat cggacgtctt gttcgtcatc gaatcgggtt ttgctccggc ctggaagtcg
     1141 ataaccatcc cgatacctgt cccccggcaa actggattca tggttacgcc actttcgttc
     1201 ccggtgatca agtcggagaa caagggcttc gttccttcga cggtttcggt tgccggcaag
     1261 cagctctcgg tggaaaccct gaccaatata gacaccatgg cccggcgaca gctgaaggat
     1321 cagatgccag cgatcctatt gcgtacggtc atccgcgcag tcgtgaaatc tgtcgctcag
     1381 gatcaagctt ataagggcgg ggtagtattg ggggtgctga cgaacgtcgc aacagtggcg
     1441 accgagcagg cggatgaacg tagctggcgc acgctgcccg atcgcattgc cgttgcgcgt
     1501 gctacgctac cgcagggcaa tcagacgatc gagtttcaaa ctggtaccgg aacattcaga
     1561 aaggaagtag ccatcggcag tcgcttcacg attatcccaa ttcgcattac cagcggaact
     1621 gtgtatatgg gtcagccgaa cacgttgggc agcggtttat ccgagctttc agctgatact
     1681 gcagtcagca agaaggcgtc gaagaaaacc gtgaagaaga ccaaccagta agaggcaagc
     1741 aggtacaaat ttctttggag aatccgatga agcgctcgat actttttggc ttgagtgtgc
     1801 tgatttcact gctgctgggt agcagcgtaa tggccgcgac cgagcgtcgt gtcgcgctgg
     1861 tgatcgggaa caatgattat cagagcattg taaaacttga aaaagcggtc aacgacgcca
     1921 acgccgttgc cgccgagttg cggaaagttg gctttgaagt cgaagtcttg aacaacgtcg
     1981 gccggaaaaa gatgaatctt gcgatcaacg acttcgtcca gaaagtctcc ggtggaggtg
     2041 tcggtgtttt cttcttcgcc ggccatggtt tgcagataaa caaccagaac tacctggttc
     2101 cgatcgatat ggatacgccg aacgatccgg ccgacgttgt cgatcaggcg gtcagtgttc
     2161 cgatcctgca ggacaaactt gccgacgcca aagccaagta taccctgctg gtgctcgatg
     2221 cctgtcgcaa caaccccttg ccaaaaaaag ccgggcgttc gattggcaca acgcgcggtc
     2281 tggcgatgac caattcaccc aacggacaaa ccgttctcta ttcggcgggt gccaatcaag
     2341 aagcgctcga cagtcttgga aacaacgaca agaacccgaa cgggctgttc acccgtgagt
     2401 tcctgccgac gatttcccag cccgggatca gcagcaccga agcgctcaag aaaacacgct
     2461 caatggtgat ccagaaagcc aagtcggtcg gtcatgaaca acaaccggcg atctacgatc
     2521 aggccgatgg cgatttctac tttgtctcgg gcccggcgcc cgcacagaga tcgacggcgg
     2581 ctggcgcagc gggaagtacg acgaccatcg tgcaggcagt tgatccgcaa gccattgaac
     2641 tatccttctg ggacaccatc aaggacagca agcagtcagc agactatcag gactatcttg
     2701 ccaagtaccc gaacggccaa tttgctccgc tcgccagtcg ccgtgttgcc gcgttgagcc
     2761 agaccgtgag tcggagcagt gatgcggtcc ctgcatcggc gtctgctcca tccgcgccgt
     2821 ccgctgtggc cagcgagccg acccaggtgg ccatgggttc ggcgccgcca ccgcccgccg
     2881 ctgctcccag tgccagtagc ggaccatcaa tggccagcaa gatcgaagaa cagggaaaga
     2941 tgaacaacct gaaggttacc gatatgaggg caaccaagcg ggataatctg ctgcgcatcc
     3001 aggtggacgt taccaacatc agttcgagca accagcagct ttactatcgt ttcaaatggc
     3061 ttgatcgcga cggcttcacg gtatgggacg aggaaccgtg gaaaccgatg attgtctacg
     3121 gcaatcaaaa acaggtcatc aatgtcgttt caccgacctt caaggcaacg gatttccggc
     3181 tcatactgca aagtccggat aacagcggca actagtccgc acaatcacgg gaagcatcat
     3241 gaaagctcta ttcggcccat ttggccgctt cacactggtt gcagcatccg ttctggcgct
     3301 ggctccaatg ctcgcatcag gaggcagtgc cagtcagacc agcaggccgg tcagccatgg
     3361 ttacgccgca ctgacacagg acgcttcggg tgatatcgcc ataagcgatc ttcgcgcgac
     3421 gatgcaggat cggttgttac gcgtccaggc cgatctgcgc aacccgacaa cgtccgaccg
     3481 gcagttgtat taccgtttcg agtggctgga tcaggacggt ctcgccgttt gggacgacga
     3541 gccgtggaaa ccaatgatcg tttacggcca gagcaaacgg acaatcagtg tttcttctcc
     3601 atcattcaag gcaacacgct tcagactggt tgtacagaac ccgaactgat atcttccggc
     3661 cagcgcgaaa gcgaccggag ggtatcgatc aacaactcta tcaggaggat ccgcgatgta
     3721 ctcgaagcca aattttgatc gcccggcaac actgcttgtc ggcaccttaa tgcttgttct
     3781 ggccggttgc cagacaacct ctcccacaac cggaacgagc ggtgtgagct acggcgattc
     3841 caaagccgtc gaaaccgtta ccaccgatct cggctcgacc gatatccaga tgatttcgga
     3901 gaagatgacc cagagcctga ttcaaactcc cgagattcaa acgctgatca agaaacgcca
     3961 gctgctgatg gcttctccag tcaagaacaa gaccagcgag tatttcgata cccgcctgat
     4021 caccgatacg gttttgacgc aactgcaaaa gaacggcgtg cgctacgcga tcgagggcga
     4081 agacatgcag aatcagaccg acgagctgcg gcgtcagagc cagtccggtc tgtatgacaa
     4141 aacaaaatcg gtcaaggtcg gcaagatgca gggtgccaag ttccgtctgg atggaagcat
     4201 ttcttccatc atcaaaaaga ccagcgatat aaaggatgtc tactacaaga tgaacctgcg
     4261 cctgatcgaa atcgagaccg gtatcgtcga atggtccgac gagaaggaaa ttcgcaaatc
     4321 agcgagacgt tgattcgatc taccgtcacg gcaacctgga gaacttgaga tgaattccaa
     4381 atggttcacc cgagtgggct ttaccctgtt cgcaacattc agcatcgctg cgtcagctgc
     4441 cacacccaag gtggccgtga ccgatctggc ctacgaagag cgggtcagtg aatattttcg
     4501 cgtcgtttca gcttcccaaa agtcatcact gcgtgcatcc gggcgtgaaa gcgagcgtga
     4561 gtcggactat gcttattcgc ggcgtagcag cggctcggtg aatgccaggt cggagagcag
     4621 ctactcgtcc accgaaggca cttacagcta tatcgaccgg ggagaaatgc gcaaattcac
     4681 tgcggacatc aagggtggga tactgaaaac gggacgtttt cagttggttc agggcaggcc
     4741 gtttaccgac gccaagaaca acgaaaagct gtatgacatc attggccgta tcaagaaggg
     4801 aatgtatccc ggcgccgatt acgtgttgtt cggtacgatc aatagtatcg aatttcgtca
     4861 ggaagccaac cccatcgacc atacgaacac gttctcgcac acattgagcc tcgaactggt
     4921 cggtgaattc agcttgatca gcaccaggaa ctttacggtc aaatcgtcgt tcagtgccat
     4981 gggtgctggc caggacgtca aaatgctgac cagtcagggc ggacgagtcg tgttgaaccg
     5041 gggcaaggtc gtttccgaag tttccaagag tcttggcgaa gacgtcgccc gacaactcga
     5101 agagcagctc agcggtgttt atggcggcaa tgaagagagc ggagagaaat atccgagctc
     5161 tcgccaggaa tcgccccgcg aagaggtgat catttttaga tagatcggtc gttggatact
     5221 cgtcccgacc gagccttgtc tgctcattgc tttgccgtac tcttcgcgtc cctgatggcg
     5281 cgcgcgtaaa gcatgttcaa tgcggccagc tcctgggttt cagatgcttt ctcgttgagc
     5341 tgcctgagca aggcctcaag cttgtcaaac tcgcgtagct cgaaataact cgccagcaga
     5401 taaagctccg gattcaggtc gccagcgacc gcctgtttgc gcatttcatc atagttcttc
     5461 tcgaccgtct ccagcctgtc gtacggaggc attgagcgca tgcggatcat gccggtcttg
     5521 acgttgccat cctgggccgg ggttttcgca agctgtttga cgagaatcgc agggagcgtc
     5581 ttgatctcag gctgcatagt gcctttaatg gctttgctcg atacgctgcc aacctcaagc
     5641 gcaccacgtc cgctccaggt ctcctgacgg cccccctcga aataaaccac ttgcaagcgg
     5701 gaagcgcctt ccatcagcaa acgatcaccc tctctcacct tgatgaaagg cttgagttcg
     5761 ctggcggctg ctttttcctc ctgcaatttg acgtttcctg aagcagcggt caccagagcc
     5821 acttcagccg ctgaagcgcc catggataag agcaccagta acaccgatcc gagccaatat
     5881 gccttatgca tccttctctc ctccctcgac gttcaagact tcaaaaacgc gaaccgcttc
     5941 ctcgcgcccc ttgactttga cgatttcact tcgcccacaa acgacgtttc gacctgctgc
     6001 gacaatcgtt gtctcgctgg ccaagatagc gcagcctatc tcctttgtca ttccttccag
     6061 tcgggaggcc agatttacag tatcgccaat cgctgtgaac tccatccgct ttagcgagcc
     6121 aatgttgccg ataaccgctt cgccgctgtg caagccaata ccgaccgcaa acttcgggag
     6181 attgcgatcg ggaaagcgtt tttccatcca gactgaaaac tcctcggcca ctgcgcgtag
     6241 cgcaatggcc gcgcgaatcc cgcgattggc gtgatcggcc agcggcaaag gcgaaccgaa
     6301 ttcaaccatg atggcatcgc cgataaactt gtcgatgctg ccaccttcgg ccaacagcgg
     6361 cgcgcaggca cgttcgaagt aggtattcag catctcgacg acttctcgtg cgtttagccg
     6421 ctcgctaatc gtcgtgaaat tccggatgtc ggagaaaaga acggtaattt cctgcgattg
     6481 gccgccaagt tccgggcggg tatccgattt cagcaaagct tcaaccacct ggtcggaaac
     6541 gtagcgaccg aacatctggc gtacccggga tcgctcgcgc tcctctcccg tcaaacgcca
     6601 gcccagtacg ccgagcaagg cgatagcagt cgctaccgca taggccgata ccggcgccaa
     6661 tacgtcgaag cgaaaagcca gaaaaccgct caaggccaac accggaatag tcgccagcca
     6721 gagcaaagcg cccgcccaga caggcagcgc gacaaatgcg acaacagcca gcccgaccat
     6781 gaggagtagc gtgccgatgc gcgggccatc gcccagaggc tgcaagcgtt caccggaaag
     6841 cagtgactca agtacgttcg catgcaattc aacgccgctc atcaggacac cccggcccga
     6901 aaagagccgt gtcgcgtaag gggtgaaatg ctcatcgttg agtcctgctg ccgtcgcccc
     6961 gataagtacc gtcttgccct gaagcgcggt aactgccggg tcgttcaagg catcatctga
     7021 caataatctc gtgagagaaa ctcgcggaaa cgttccaggt gggccgaagt agggaattgg
     7081 ctcgggcggc tgctctcgat ccatcgcgcg acctccaatc atccagcttg tggccagagg
     7141 gtccaacccg gccgcgcgaa ccgcgaggag agacggcagg ccgagtatcg gtacgtttcc
     7201 atccagcaga attcggtcgg ccagtgcaac cggtgccatc agatagcggc gaatgacccc
     7261 atcaccctca tcaagcaggt cggccagtgc aacgtaaccg gggatatcaa aatcaggaag
     7321 tgccagcaga taatccggac tcggtagcaa gtagtccgtc tcgcgtccgc ccgatcccga
     7381 gcgggtcgat gccaacacca gttgcccact attgatttgc tcgcggaaag tgcggtcata
     7441 gtcccgcgct gcctgttgca ggtcgccacc cagtttgccg agccaacgtt cggggctgat
     7501 gctgagcagc atgtcgagtc cgaccacgtt agcgcccact tcgcgcaagc gtgccacggc
     7561 gacggccagt cgatcagtcc agaaaatcat cgggtcatcc ggatacgccg cgagggtatc
     7621 ctcgtcaagg gcgacaatac cgacgtgtcg agcttccgcc cgtacgccct gccactgaaa
     7681 ccagaaatcc tgcagccgtt gatcaaccgg gttgagtacg ccgccacgca gcaaagcttc
     7741 ggcgatcagg caggccgcca gcgcaaaaaa caccaaccag agcctgcggc ttttgcgcgg
     7801 gcggccgagc atcatcactc caaattctgg atttgttcgc gcatttgctc gatcagcagt
     7861 ttcagttcca tcgccgtctg ggaaacttca ctgaccaccg atttggaacc cagggtattg
     7921 gcctcccgat tgagttcctg catcaggaaa tcgaggcgtt taccgcaagc gccgcccgct
     7981 ttcagcacgc gctcaacctc gtcaaggtgg gtagacagac gcgccagttc ttcggcgacg
     8041 tcgatacgca cggcgaaaac agccacttcc tgtcgaatac gttcgtcgtc gaccgagtgg
     8101 atcgcatcga acaaacgctg cttgagcttc tcctggaatg ccgcctgagc tgcagggatc
     8161 cggggggcta cttcgcgaac cagttcgcgc atgcgggcta cccgctcgag aatcacggcg
     8221 gccagttttt caccctcgcg cgcgcgactg gcgctgaagt cctccagcac atctttggcc
     8281 acgatcagac agtctggaag cagagcttcg atgtccagcg cgtcttcggc aaacatcccg
     8341 ggccagcgca acacatcgct tacggcaagc ggcgcagcat ttaccagctc gctgcgaatc
     8401 ctgtcgtcaa aggacttcag gcgctggagc agttctacgt tgagtacaag cgattgagcg
     8461 tgcgccgtcg ctgaaacaaa attaagtcgg cattcaagct tgccgcgagt cagtcgtgaa
     8521 gcgaacagtt cacgcagtgc cggttcgaca atgcgcaatt catcgctcag acgaaactgg
     8581 aaatcaaggt agcgcgaatt gacgcttttc agctcgatat gcagcgcgcc acaatcaacg
     8641 tcaagcgttc tcgaggcata cccggtcatg ctaaagatca tttgagtcct gatgaatttg
     8701 gtggcgagtt agcggtttgc tttacagtgc actgccaaag cctgaaaatg gccagaaaac
     8761 cacatgatag cttcgatatt gatggcgcaa caagcgaacc acgctctccc ttccggcttc
     8821 caactcgatg tatatcacat cgagcatcag ctttctttgg gcgggttttc gatcgtctat
     8881 ctggccaccg atcccgaggg caagtttgtg gccatcaagg aatatctgcc aaatagtctc
     8941 gctttgcgtg gcgagggcgg aatcaaaccg gtcatcagtg acgagcacat gccggccttt
     9001 cgctatggaa tgaagtgttt ttttgaggaa ggtcgcgcgc tggccaagct ttcccatccg
     9061 aacgtaatcc gcgtgttgaa cttcttccgg ggaaatgaca cggtctatat ggtgatggaa
     9121 tacgagcagg ggcgtacgct gcaagagttc attcagaaga acagggcggt ggttaccgaa
     9181 aacttcatcc gcaacgtatt taccaaactg ctcaatggtc tgcgcgaggt tcacgcgcac
     9241 aaactgctgc atctcgacat caagccatcc aatgtctaca tgcgtaacga ctataccccg
     9301 gtattgatcg atttcggcgc cgcgcgacag acactggcca gtgacacgcc gatgctcaag
     9361 ccgatgtaca caccgggttt tgcttcaccg gagcattaca agcagcgcga attgcttggc
     9421 ccctggagcg atatctatag tgtcggcgct tcgatgtatg cgagcctggc tgcaaccgct
     9481 ccacaatcgg ccgatagccg gcaggaaaag gacaaagtaa ttcccgccat gatacgttgg
     9541 caaggtcaat actcggagca attgctcaaa acggtagatt ggtgtttgtc gcttgatcat
     9601 ctgagtcggc cgcaaagcgt ctttgccctg caaaaagcgc tgacaaagaa ggtccccaaa
     9661 ccggaactgc ggaaaaagca ctggcttgac cacgtggtag gaaaattcaa gagaaagatt
     9721 gaggtccagt gaaattcaca atttatcaag aaagccgacc aggcaagcgc gccaacaacg
     9781 aggacagact ggcccattgc tattcgcgtg atgccctgtt gctggtcatt gcagacggaa
     9841 tgggcgggca ctactacggc gaggtcgcag cgcaaatcgc cgtacaggta ctcgtcgaaa
     9901 cctttcagcg tgacgccaaa cccgagattc gagatccctt catgttcttg cataaggcga
     9961 tgaccaccgc gcatcgtgcg atcgtcgatt ttaccgatgc tcacaagctc aaggattcgc
    10021 cgcgaacaac ctgcgtggcc tgcatcatcc aggacaacat tgcctattgg gcacatgcag
    10081 gcgactcacg actttatcta ttgcgtggtg gaaaagtggt tactcacacg cgcgatcact
    10141 cgcgcgttca gttgctgatt gatcagggct tgatcaacga gtcacaagcg gccgtgcacc
    10201 cggaccgcaa caaggtctat agctgccttg gtggtcaaca gtctcccgag atcgagtttt
    10261 cgcgcaaaac acctcttgtg gctggcgatg tgctggcgct ctgcacagat ggcgtgtggg
    10321 gtgtcatacc ggacgatacg ctggccgtcg ctctgaaaga gaaaaatctg atgcaggcgg
    10381 tgccgatgct gcttggccag gcggatatcc gaggcgggcc gaacgccgac aacctctcga
    10441 ttgtcgtcgt gcgctgggaa gacagttatg
//
LOCUS       DAORPM010000064        10435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1455_length_10435_cov_10.458574, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000064 DAORPM010000000
VERSION     DAORPM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1455_length_10435_cov_10.458574"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..828
                     /locus_tag="PLD53_04730"
     CDS             157..828
                     /locus_tag="PLD53_04730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC60246.1"
                     /translation="MNDKPMKLIQCWDDGVTTDTRLVDILRRHGAKATFNLNAGLHER
                     RRRLDWIYQDTEVWKLGMDEMREVYDGFSIANHSLTHPHLDEIPVDAARREIAEGRDR
                     LEQFFGKSVTGFAYPFGTYNEAVMSMVSETGHLYARTVQVATHPFPPVDAMAFHPCCH
                     FLAPDFWERYEQARDGGVFYFWGHSYEMVSESMWASFDEAIARMSHDPMTCWQNIAEL
                     FAEAP"
     gene            970..1257
                     /locus_tag="PLD53_04735"
     CDS             970..1257
                     /locus_tag="PLD53_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRC60247.1"
                     /translation="MASVGYHEPVEELSDETRDMHRAIVSLMEELEAVDWYNQRANAC
                     TDPDLKAILEHNRDEEKEHASMVLEWIRRKDQTFSKELKDYLFTEKPIAHK"
     gene            1405..1560
                     /locus_tag="PLD53_04740"
     CDS             1405..1560
                     /locus_tag="PLD53_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60248.1"
                     /translation="MILIIHAHPYPQRSRAGRAFLEAVRDLRPAGETLPLGGEQRRGG
                     GVVSALS"
     gene            complement(1627..2994)
                     /gene="glmM"
                     /locus_tag="PLD53_04745"
     CDS             complement(1627..2994)
                     /gene="glmM"
                     /locus_tag="PLD53_04745"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRC60249.1"
                     /translation="MTRKYFGTDGIRGLVGQSPITPDFVMRLGYAAGKVLVEDPSARG
                     HLQSGDRPAVLIGKDTRISGYMLEASLEAGFSAAGVDVCLVGPIPTPAVAYLTRALRL
                     QAGIVISASHNPFYDNGIKFFSAQGTKLEDALEAKIEAGIESPLVCVPSAELGRARRI
                     DDAGGRYIEFCKSTFPNALDLRGLKIVVDCAHGAAYNIAPHVFHELGAEVIAIGAEPN
                     GLNINKDVGATATGALSKAVLAHQADLGIALDGDADRVMMVDATGVVYDGDQLLLAVV
                     RSRLRQGPMAGVVGTLMTNLAFEHALEGLNVPFARAAVGDRYVLEMLNEKGWLFGGEN
                     SGHILCLDKHTTGDGIVSALQVLSALCEEGGDLAGLLGRLVLYPQVLINVPLVKGFPW
                     KDHPLIASVLRDVETALEGEGRVLLRASGTEPLLRVMVEGQNAGKVQGAAEKLAAAVR
                     EAVSA"
     gene            complement(3151..>3846)
                     /locus_tag="PLD53_04750"
     CDS             complement(3151..>3846)
                     /locus_tag="PLD53_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRC60250.1"
                     /translation="VAAASRVLDIGWNNLQWLVAALSVGLGFGLQEIFANFVSGLIVL
                     AERPIRIGDVVTVGDVTGTVARIRARATAVIDFDNKEVIIPNKAFITDRVINWTLSTG
                     TTRLLIKVGVAYGCDTALVQKLLLEVVQANDDVLEQPSPSVYFIDFGDSSLNFEIRAF
                     VDAFDKRLRVQHEINTAIDGVLREHGIEIPFPQRDLHIRSAEGLAGLPVSPAAKTETL
                     ASQTAANSAQASV"
     assembly_gap    3847..3946
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3947..5678)
                     /locus_tag="PLD53_04755"
     CDS             complement(<3947..5678)
                     /locus_tag="PLD53_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60251.1"
                     /translation="MTRQARDRALATTSDEILRYERELDDLSDREESPARYYPAQRSS
                     SRVAERTKIDSSTRATMTEQRRLLAQLIEIDGALLKTLLESKKTERELMQVSEAAHTQ
                     LSKMLFWVPAQFSAQTFVELRSAGAWTVSWENWRGALTNLLVEVSAELVWPVFLGFCV
                     LLLLVTRSRLKRILAALAPSSGRYRPYRIGHTVAALLITLALALPGPVVFWAISLLLG
                     MADESQIFSHALAAALAAAAKLLLATSVFARLIDQNGVAVAHFGWDEPSLNFSARALR
                     RFSVVFVLLIFLASLNGLDYAPYANRESLGFLAFILAMLALAGFFAYLFKTSSPPVRR
                     LIIYQPHSWMLRFHKLWYFVLIAVPLSVAGLALAGYYAAAGYFFSRVLYSAFFVIGAI
                     TLYGLIALWVQTEHARLERLQREEKAKSEAEVEAEAEAEEDGEVAIVVPHLDVATIGE
                     QTRSLLDFFITLFLLGGLWSVWKGALPVLSVIGDYSLWTSRTIADGKEVVFSLSVGHV
                     FLALLVASITTVAVRNVGALLDIVLLKRLEMRADATYAIKVVTRYALTAIGVAAASRV
                     LDIGWNNLQWL"
     gene            5727..6134
                     /locus_tag="PLD53_04760"
     CDS             5727..6134
                     /locus_tag="PLD53_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60252.1"
                     /translation="MNKLAAQDLDLRPLLDPFQTFFEYLLALMKIRKLAARFVEQCLA
                     LGDPAQQVGIVCCDPLLWVDGLYLRLPHRLRMDEAAKNQERQCKQEGSECGATDDARS
                     LNRGKRKTHDQRIASVMPASALMDTSPGLASSH"
     gene            complement(6373..8217)
                     /gene="typA"
                     /locus_tag="PLD53_04765"
     CDS             complement(6373..8217)
                     /gene="typA"
                     /locus_tag="PLD53_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRC60253.1"
                     /translation="MSRSVRNIAIIAHVDHGKTTLVDKLLHQAGSFSAHQHIAERVMD
                     SNDLEKERGITILAKNLAVDYQGVHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTKKALALGLKPIVVVNKVDRDGARPDWVVDHTFDLFDKLGASNEQMDF
                     PVIYASALNGYATLDLAKPSLDMRPMFDAILEHVPPPHADEIDGPLQLQVSALDYSSY
                     VGRIGIGRIQRGRLKPAQQITVMYGQADADGKFEHGLPKTAKVNQVFGFRGIERVSLD
                     EAVAGDIVLVTGIDDLSIGCTITDNDKPEALPMLRIDEPTLNMTFMVNNSPYAGTEGK
                     FVTSRQIRERLTKELLTNMALRVEDTKDTDSFLVSGRGELHLTILLETMRREGFELAV
                     GRPKVIYKVIDGVECEPYEMLTLDVEEAHQGGIMEALGYRKGDLQDMVSDGRGRVRLE
                     YRVPARGLIGFQGECMNLTRGTGLMSHVFDDYAPVKGDIPGRRNGVLISAEKGEAVAY
                     ALWKLQDRGRMFTVPGDRLYEGMIIGIHSRENDLVVNPIKGKQLTNVRSSGTDEAVRL
                     VPPVELTLESAVEFIDDDELVEITPKCIRLRKRHLIEHERKRALRAEG"
     gene            complement(8340..>10435)
                     /locus_tag="PLD53_04770"
     CDS             complement(8340..>10435)
                     /locus_tag="PLD53_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRC60254.1"
                     /translation="TRAEMDELGWDSCDIILVTGDAYVDHPSFGMALVGRLLEAQGFR
                     VGIISQPDWLSAGAFRALGKPNLFFGVTAGNMDSMVNRYTSDRKIRSDDAYTPHAEAD
                     KRPDRAIVVYAQRCREAYREANVVIGSIEASLRRIAHYDYWSDKVRRSVLPDSKADML
                     VFGSAERAIVALAHRLAAGESIAEIRDLRGTAFMVPRGWLPAGDWSEIDSSEVDTPGL
                     LIRHADPYAFEPDKGNEAICASKTEVATAPTAKVIQFHSHTRMPKLDRTRTAIRLPSY
                     EQVVNDPVLYAHASRTFHIESNPGNARALIQGHGERDVWLNPPPLPLTTAEMDEVFAS
                     PFQRKPHPSYGDAKIPAFDMIRFSINIMRGCFGGCTFCSITEHEGRIIQSRSEASILR
                     EIEEIRDKAPGFTGTISDLGGPTANMYRLACKNPKIESSCRLLSCVFPGICENLNTDH
                     APLIQLYRKARAIKGVKRVLVSSGLRYDLALRSPDYVKELVTHHVGGYLKIAPEHTED
                     GPLAHMMKPGIGTYDQFKLLFDKYSKEAGKEQYLIPYFIAAHPGTTDKDMLNLALWLK
                     ERNFRLDQVQTFLPTPMAIATAMYHTQRNPLKKVTRDSQSSAAVETVRASRMRKLHKA
                     FLRYHDPENWPLLRDALRQMGRADLIGSGKKHLIPGFQPVGTGLRHQETGTLAKTGNR
                     KMRTTKPPRTPRCTK"
BASE COUNT         2260 a         3105 c         2888 g         2082 t
ORIGIN      
        1 cttcacggct ggaacagttc gtcgcccttc gcccgcccga acaacatcca ctgttcaagt
       61 gtgttccggg ccccgtcgcc gagtgggaag tcttacggcc ccactcatga gagctgatct
      121 atccgttgcg cagtttgaac ttctttgaga actggaatga acgacaagcc catgaagttg
      181 attcaatgtt gggatgacgg cgtcaccaca gacacgcggc tggttgacat tctccgacgc
      241 cacggcgcaa aggcaacgtt caatctcaac gcaggcctcc atgagaggcg tcgccgactc
      301 gattggatct accaggacac tgaagtatgg aaactcggga tggatgaaat gcgtgaagtc
      361 tacgatggct tttcaattgc gaatcacagc ctgacgcacc cgcatcttga tgaaatccca
      421 gttgatgctg cccgccgcga gatcgccgaa gggcgcgacc gtctggagca attcttcggc
      481 aaatccgtca ccggattcgc ctatccgttt ggcacataca atgaagccgt gatgtctatg
      541 gtaagtgaaa caggacacct ttacgcacga acggtacaag tggctacgca cccattcccg
      601 cccgtagacg cgatggcttt ccatccttgc tgccatttcc ttgcgccgga tttctgggag
      661 cgctacgagc aagccaggga cggtggcgtc ttttattttt ggggccactc gtacgagatg
      721 gtcagcgagt ccatgtgggc tagctttgat gaggccatag ctcgcatgag tcacgatccc
      781 atgacctgtt ggcagaatat cgcggaactg tttgcagagg cgccttagtt ctggctttcg
      841 catgtcgcca cttgcaagct cacaaactcg attgcaggtc caacaacaat tgcttgctga
      901 agtcagcgca tcgggacgac ccggactcaa gtatgatgaa atcacatctc aactgcggag
      961 gaactactca tggccagtgt tggatatcac gaacccgttg aagaactgtc cgacgaaacg
     1021 cgtgacatgc accgcgcaat cgtttcactg atggaggaac tggaggcggt agactggtac
     1081 aaccagcgtg caaacgcctg cacggatcct gacctcaaag ccatactcga gcacaatcgc
     1141 gacgaagaga aagagcatgc ctccatggtt ttggaatgga ttcgccgcaa ggatcaaacg
     1201 ttttccaaag agttgaaaga ctatctcttt accgaaaaac ccatcgcgca caaatagcaa
     1261 tattcggata ggtctgcgca acggcgcagc tttgccgttg caggctttct gcagcgccgt
     1321 tcaggcgacc agccaaccgt gagcggtgga tggtcggcgt tgtccagata gccgattctc
     1381 gggatagtgc gacattcttc gccgatgatt ctgatcatcc acgcgcatcc ttatccgcag
     1441 cgttcccgcg ctggccgcgc cttcctggag gccgtgcgcg atttgcgccc tgcgggggaa
     1501 acactgcctc tgggtggcga acagcggcgg ggaggaggcg tcgtcagcgc gctcagctaa
     1561 tctgcctcgt agctcttgcc acctcaagaa ccccgcacag tggcgggaat tttcgtccaa
     1621 gcaggattag gctgaaacag cctcacgcac ggctgccgcg agtttttctg ctgccccctg
     1681 caccttcccg gcattctgcc cttcgaccat gacccgcagc agcggctcgg ttcccgaggc
     1741 gcgcagcagt accctgccct caccttccag cgccgtttca acgtcacgca gcaccgaggc
     1801 aatcaacggg tgatccttcc atgggaaacc cttgaccagt ggcacattga tcaacacttg
     1861 cggatacagc accagccgac caagcaagcc cgccagatca ccgccctcct cgcacagcgc
     1921 cgaaagcacc tgcaaggccg atacgattcc gtcgccggtg gtgtgtttgt cgagacacag
     1981 gatgtggccc gagttctcac cgccgaacaa ccagcccttt tcattcagca tttccagcac
     2041 gtaccggtca ccgacagccg cacgcgcaaa aggcacgttc aatccttcga gggcgtgctc
     2101 aaaggccaga ttggtcatca gcgtcccgac cacgccggcc attggcccct gccgcagacg
     2161 gctacgcacg acggccagga gcagttgatc gccgtcataa acaacgcctg tcgcatccac
     2221 catcatcaca cgatccgcat cgccatcgag cgcaataccc agatcggcct ggtgcgccaa
     2281 aaccgccttg ctcaaggcac cggtcgccgt tgccccaaca tccttgttga tattcaggcc
     2341 attcggttcg gcgcctatcg cgatcacctc ggcccccaac tcgtgaaaga catgcggtgc
     2401 aatgttgtag gctgcgccgt gtgcgcaatc gacgacgatt ttcaatcctc gcaagtcaag
     2461 agcattcgga aaagtgctct tgcagaactc gatgtagcgt ccacccgcat cgtctatccg
     2521 gcgagcacga cccagctctg ccgatgggac gcaaaccaga ggcgactcga tgccggcttc
     2581 gattttggcc tccagggcgt cctccagctt ggtgccttgc gctgaaaaaa acttgatacc
     2641 gttgtcgtag aacggattgt gcgacgctga aatgactatc ccggcctgca agcgcaaggc
     2701 tctcgtcaga taagccacag cgggcgttgg tatcggcccg accagacaga cgtcgacccc
     2761 ggcagcagag aacccggctt ccagcgaagc ttccagcatg tatcccgaaa tccgggtgtc
     2821 cttcccgatc aacacggccg gacgatcgcc tgactggagg tgaccacgcg ccgacgggtc
     2881 ttcgacgagc actttgccgg ccgcgtatcc gaggcgcatt acgaaatccg gagtaatcgg
     2941 agactgccca accagaccac gaataccgtc ggtgccgaaa tattttcttg tcatttaccc
     3001 actcccctgt atgctcttgg tcgcaccaga atcgctaggt gcgaaagact ggcattgtac
     3061 tgaaacctat cgctgttggc ctaccgaaaa gccacgggca aagtgaacgc atgcatgcca
     3121 gcgtcgcaaa cagtcgcaag ccacatacgg tcaaacgctc gcttgggcgc tattcgcagc
     3181 agtttgactt gcgagggttt ccgtctttgc tgcgggacta acgggcaggc cagccagacc
     3241 ttctgccgaa cgaatatgca gatcacgctg cgggaagggg atttcgatcc catgctcacg
     3301 gagaactcca tcaattgcgg tgttgatctc gtgctgcaca cgcagtcgtt tgtcgaaggc
     3361 atcgacaaac gctcgaatct cgaagttcag cgagctatca ccaaaatcga tgaagtagac
     3421 cgaaggcgaa ggttgttcga gcacgtcgtc attggcctgc acaacctcaa gcagcaattt
     3481 ctggacgagc gcagtatcgc acccgtaagc gacgccaacc ttgatcagta gccgcgttgt
     3541 tcctgtcgac aaggtccaat tgatcacgcg atccgtgatg aatgccttgt tcggaatgat
     3601 aacttccttg ttgtcaaaat ctatgacagc cgtcgcccgg gcacgaatcc gggccacagt
     3661 tccggtcaca tcgcccacgg tgaccacgtc gccgatacgt atcgggcgct cagccaggac
     3721 aatgaggccg gaaacgaaat tggcgaagat ttcctgcaag ccaaaaccca ggccaacact
     3781 cagagcggca accagccact gcaggttgtt ccagccaatg tcgagtacgc gtgacgcggc
     3841 ggcaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccag ccactgcagg
     3961 ttgttccagc caatgtcgag tacgcgtgac gcggcggcaa cacctatcgc ggtgagtgcg
     4021 tagcgggtga cgaccttgat cgcataggtt gcatccgcac gcatttcgag tcgcttcagc
     4081 aacacaatgt cgagcagcgc gcccacattg cgaaccgcaa cggtagttat cgaagcaacg
     4141 aggagcgcca gaaagacatg gcccaccgaa aggctgaaaa ccacctcttt cccatcagca
     4201 atcgtcctcg atgtccatag cgagtagtcg ccaatcaccg acagcacagg caaagcgcct
     4261 ttccatacgc tccagaggcc gccgagcaaa aacagggtga tgaagaagtc cagcaaagaa
     4321 cgcgtttgct cgccaatcgt cgcaacatcc agatggggaa cgacaatcgc cacctcgcca
     4381 tcttcttccg cctccgcctc ggcttcgacc tctgcctccg atttggcttt ttcctcgcgc
     4441 tgcagccgct ccagtcgcgc gtgctcagtc tggacccata gggcgatcag gccatagagt
     4501 gttattgctc caatcacaaa aaacgccgag tacaacacac ggctgaagaa ataacccgct
     4561 gccgcgtagt atccggcgag cgccagtccc gctaccgaca acggcacggc aatcaagacg
     4621 aaataccaca gcttatgaaa acgcagcatc cagctgtgtg gctgatagat aatcaaccgt
     4681 ctcacgggag ggctgcttgt cttgaacagg tacgcaaaaa aaccggccag cgccagcata
     4741 gctaaaatga aggcaagaaa accaaggctc tcgcggttgg catacggggc atagtcgagg
     4801 ccattcaggc tggcaagaaa gatcagcagc acgaagacca cagagaacct acgcagcgca
     4861 cgcgccgaga aattcaaaga gggttcatcc cagccaaaat gggcaaccgc cacgccattc
     4921 tgatcaatga ggcgtgcaaa aactgaagtc gccagcaata gttttgctgc tgccgccaac
     4981 gctgcggcca acgcatgcga gaatatctgc gattcgtcgg ccataccgag caggagagag
     5041 atcgcccaga acacgaccgg tcccggtagt gccagggcaa gggtgatcaa gagcgcggcc
     5101 accgtatggc caattcgata cggtcgataa cgtcccgagg atggcgccaa tgcagccagg
     5161 atacgcttca ggcgggagcg ggtaacgagg aggagaagca cgcagaatcc tagaaacact
     5221 ggccagacca gttcggcgga tacttccacc aagagattgg tcagcgcgcc tcgccagttt
     5281 tcccacgaaa cggtccacgc gccggccgag cgcagctcca caaatgtttg tgcgctgaac
     5341 tgcgcaggca cccagaacaa catttttgac aactgagtgt gtgctgcctc actcacctgc
     5401 atgagctcgc gttccgtttt tttcgactcc aacagcgtct tgagcaaggc gccatcaatt
     5461 tctattaact gcgccaacaa tcgtcgctgc tcggtcatgg ttgcgcgggt gcttgaatcg
     5521 attttcgtac gctcagcgac ccggctcgaa gaccgctgcg ccggataata gcgcgctggt
     5581 gactcctccc tatcgctcag gtcgtccagc tcacgttcgt aacgcaggat ttcatcgctc
     5641 gttgtcgcca gtgcacggtc gcgtgcctga cgcgtcaact tgtaatgtgc gggctttgga
     5701 aggtgacgca actcttcaag tgccgcttga acaaactcgc ggcccaagac cttgacctgc
     5761 gcccgctgct cgatcctttc caaacctttt tcgagtatct gcttgcgctg atgaaaatac
     5821 gcaagctcgc tgcgcgcttc gtcgagcaat gcctcgcgct cggcgatcct gcgcagcaag
     5881 taggcattgt ttgttgcgat ccgctcctct gggtcgatgg tctgtacctc aggctgcccc
     5941 acaggctgcg catggacgag gccgccaaga accaggaaag acaatgcaaa caggagggta
     6001 gtgagtgcgg agcgaccgac gatgcacggt ctttgaaccg aggaaaacgg aaaacacatg
     6061 atcagcggat cgcctcggtc atgcccgcaa gcgcgctcat ggatacttcg ccgggcctgg
     6121 cgtcgagcca ctgagcatcg atcagcccct gaaaatccgc tttcatctgc atactttacg
     6181 cgtagctccc cacagctaaa tgcaaaacgt ggaagacagg caaatcgtac gatcagcccg
     6241 gagtatccgc gacccttcaa ccccggtcaa cgccaaacgt catgccgaca tgccggcgcg
     6301 gcgtgaagcg gatgacctcc tgagaactac gcggagtccc gtgcgagcgt ccgggccacc
     6361 actgaggctg acttaccctt cggcgcgcag ggcgcgtttg cgttcgtgct cgatgaggtg
     6421 acgtttacga aggcggatgc acttgggcgt gatttcaacg agttcgtcat cgtcgatgaa
     6481 ttcgacggcg ctttcaagcg tcaactccac cggaggtacc agacgcacgg cctcgtcggt
     6541 gccggaggat cgaacgttgg tcagttgttt gcccttgatc ggattgacca ccagatcgtt
     6601 ctcgcgcgag tgaatcccga tgatcatgcc ttcgtacagg cggtcacccg gcacggtgaa
     6661 catgcgcccc cgatcctgca acttccatag cgcataagcc acagcttcgc ctttttctgc
     6721 cgaaataagc acgccgttac gacggccggg aatatcgccc ttgaccggtg cgtaatcgtc
     6781 gaatacatgg ctcatcagtc cggttccgcg cgtcaggttc atgcactcgc cctgaaaacc
     6841 gatcaagccg cgtgccggca cccggtattc gagacggacg cgaccacggc catcggaaac
     6901 catgtcctgc aaatcgccct tgcggtagcc gagcgcttcc atgatgccgc cttggtgcgc
     6961 ctcctcgaca tccagcgtga gcatctcgta gggttcgcat tcaacgccgt caattacttt
     7021 atagatcacc ttcgggcgac cgaccgccag ctcgaaacct tcgcggcgca tcgtttcaag
     7081 cagaatggtc aaatgcagtt caccgcgacc ggagaccagg aaggaatcgg tgtccttggt
     7141 atcctcaacg cgcagcgcca tgttggtcag cagctccttg gtcaggcgct cgcggatctg
     7201 gcgactggtc acaaacttgc cttcggtgcc ggcatagggc gagttgttga ccatgaaggt
     7261 catgttcagc gtcggctcgt cgatcctcag catcggcaag gcttcgggct tgtcgttatc
     7321 ggtgatcgtg cagccgatcg agaggtcgtc tatgcctgtc accaggacga tatcgccggc
     7381 caccgcttcg tccagcgaaa cccgttcgat accgcgaaac ccgaagacct gattgacctt
     7441 ggccgttttc ggcaggccat gttcgaactt gccgtcggcg tcggcctggc cgtacatcac
     7501 ggtgatctgc tgcgccggct tcaggcgacc gcgctgaatg cggccgatgc cgatgcggcc
     7561 aacgtaggac gagtaatcga gtgcggaaac ctgcagttgc agcgggccgt cgatctcgtc
     7621 ggcgtgcggc ggcggaacat gttcgaggat tgcatcgaac atcggccgca tgtcgagtga
     7681 gggtttggcc agatcgagtg tcgcgtaacc attgagcgcc gaggcgtaga tcaccgggaa
     7741 atccatctgc tcattggatg cgccgagctt atcgaaaagg tcgaaggtgt gatcgaccac
     7801 ccagtccggt cgcgcaccat cacggtcgac cttgttgacc acgacaatcg gcttcaggcc
     7861 cagcgccagc gccttcttgg tcacgaagcg cgtctgcggc atcggccctt cgacggcgtc
     7921 gaccaacagc agcacaccat cgaccatcga cagcacgcgc tcgacctcgc cgccgaagtc
     7981 cgcgtgtcct ggcgtatcga cgatattgat atgcacgccc tgatagtcga cggccagatt
     8041 cttggccagg atcgtgattc cccgctcttt ttccagatcg ttcgaatcca tcacgcgttc
     8101 ggcgatgtgc tggtgggctg aaaaacttcc tgcctgatgc agcagcttgt cgacgagagt
     8161 cgtcttgccg tggtcaacgt gggcaatgat ggcgatgtta cggacggaac gggacataag
     8221 gggctaacgc tttgaaaaaa ggggctgatt ctagcacaga tgctttggaa gaaggaccaa
     8281 aactccacca ccaagacacc aagaacacaa agttccacca agaaaataga ttctttatcc
     8341 tacttggtgc atcttggtgt tcttgggggc ttggtggttc gcatttttcg atttccggtt
     8401 tttgcaagcg tacccgtttc ctgatgcctc aatcctgtgc ccaccggttg aaaacccggg
     8461 atcaggtgct tcttgccgct gccgatcagg tccgcacgac ccatctgtcg cagtgcatcg
     8521 cgcagcaagg gccagttttc gggatcgtga tagcgcagaa aggccttgtg cagtttgcgc
     8581 atgcggctgg cgcgaacggt ttcgacggcc gcggagctct gcgaatcacg cgtaactttc
     8641 ttcagtggat tgcgctgcgt gtgatacatc gcagtggcga tggccatcgg tgtcggcagg
     8701 aaggtttgca cctggtcgag ccggaaattg cgctccttga gccacaaggc cagattgagc
     8761 atgtccttgt cggtcgttcc cggatgcgcg gcgatgaaat aaggaatcag gtactgttcc
     8821 ttgcctgcct ccttcgaata tttgtcgaac aacagcttga actggtcgta agtgccgatg
     8881 cccggcttca tcatgtgcgc cagcggcccg tcttcggtat gttccggggc gattttcaaa
     8941 tagccgccga cgtgatgcgt caccagttcc ttcacgtagt ccggtgaacg cagggcgagg
     9001 tcataacgca ggcccgaact gaccagcacc cgcttcaccc ccttgatcgc ccgcgctttg
     9061 cgataaagct ggataagcgg cgcgtggtcg gtattcaggt tctcgcagat gccgggaaat
     9121 acgcaggaca ggaggcggca tgaggactcg atcttcgggt tcttgcacgc cagacggtac
     9181 atattcgccg tcggtccgcc gaggtcggaa atggttccgg taaaccccgg cgccttgtcc
     9241 cggatttcct cgatctcgcg cagtatcgac gcctcggaac ggctctggat gatgcgtcct
     9301 tcgtgttcgg taattgagca aaaggtgcat ccgccgaagc agccgcgcat gatattgatc
     9361 gaaaaacgga tcatgtcgaa cgccggtatc ttcgcatcgc cataggaagg atgcggtttg
     9421 cgctggaacg gagaggcaaa gacctcgtcc atctccgcgg tggtcagcgg caacggcggc
     9481 ggattgagcc agacgtcgcg ttcgccgtga ccctgaatca gggcgcgggc gttgcccgga
     9541 ttcgattcga tgtggaaggt tcgcgaggcg tgcgcataca ggaccggatc gttcaccacc
     9601 tgctcatagg acggcagacg tatcgccgtt cgggtccggt ccagtttggg catccgggta
     9661 tgcgaatgaa attgaattac tttggccgtt ggtgctgttg ctacctctgt tttcgaggca
     9721 caaattgctt cgttgccctt gtcgggctcg aacgcatagg ggtcggcatg acggatcaac
     9781 agtccgggcg tatcgacttc gcttgaatcg atctccgacc aatcgccagc aggcagccag
     9841 ccgcgcggaa ccataaaggc tgtgccgcgc aaatcgcgaa tttcggcgat cgattcgccc
     9901 gcggccagcc ggtgcgccag cgccacgatg gcgcgctcgg cgctgccgaa aacgagcata
     9961 tcggcttttg aatccgggag gaccgatcgc cgcaccttgt ccgaccagta gtcgtagtgc
    10021 gcaatgcggc gcaagctggc ttcaatcgaa ccgatcacca cgttggcttc gcgataggcc
    10081 tcgcgacagc gctgcgcata gaccacgatg gcccgatccg gccgcttgtc ggcttcggcg
    10141 tgcggcgtat aggcatcgtc cgagcggatt ttccgatccg aggtgtagcg attgaccatc
    10201 gaatccatat tgccggcggt cacgccaaag aacagattgg gcttgcctag ggccctgaac
    10261 gcgccggccg acagccagtc gggctggctg atgataccga cgcgaaaacc ctgtgcctca
    10321 aggagacggc cgaccagcgc catgccgaaa ctcggatgat cgacgtaagc gtcgccggtc
    10381 accaggatga tgtcgcagga atcccagccg agctcgtcca tctcggcgcg cgtca
//
LOCUS       DAORPM010000065        10431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1457_length_10431_cov_10.250771, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000065 DAORPM010000000
VERSION     DAORPM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_10431_cov_10.250771"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLD53_04775"
     CDS             complement(<1..809)
                     /locus_tag="PLD53_04775"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRC60255.1"
                     /translation="MMLKQPATKYTAFPPVDIEDRQWPSRTITTAPVWMSTDLRDGNQ
                     ALFEPMNAERKMRMFKTLCEVGFKEIEVAFPSASQTDFDFVRKLIEEKHIPDDVTIEV
                     LAPAREQFIRRTFESLKGARRAIVHIYNATSKPFREIVFNMSKAEVVAMAVTAVKLVK
                     QLAEQNPETEWVLEYSPETFTATELDFALEICNAVTDAWGATPDNKIILNLPTTVERA
                     TPNIYADQIEWMHRHLARRDSILISLHPHNDRGTAVAATELGLMAGADRVEG"
     gene            984..1445
                     /locus_tag="PLD53_04780"
     CDS             984..1445
                     /locus_tag="PLD53_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRC60256.1"
                     /translation="MEIDRFDRQILQILQVDGRISNQDLADRIGLSPSPCLRRVRTLE
                     DSGVITGYRALLNAKALGYSLMALIYISMDKHTPERFEHFEKDISQISEVLECLLVTG
                     QDADYQIKVVVRDMDAYQELLLNRITRIQGVTGVHSSFVLRRVVDKTALPV"
     gene            complement(1504..2169)
                     /locus_tag="PLD53_04785"
     CDS             complement(1504..2169)
                     /locus_tag="PLD53_04785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC60257.1"
                     /translation="MQTAINASLYPEMAFSLDGLTSRPFDGGYQLLPSLRPLWGLLPR
                     SRGYNIRPVRTPHQHGMASMLVQQMYTWRGYSTEAVGLRPDDPNRVTLAAWQYDEVVA
                     TLTLGRDSPDGLLADSLYGPELASLRRPGRVVCEVTRLAVDPDFSSQGLLTALFQVAR
                     NYGKDMFAASDAVIEVNPRHARYYQRTLGFRQIGELRQCQRVEAPAVLLHRRLDDLAT
                     ATD"
     gene            complement(2202..2819)
                     /locus_tag="PLD53_04790"
     CDS             complement(2202..2819)
                     /locus_tag="PLD53_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRC60258.1"
                     /translation="MFNKPSVFGRNEPITRRDSQPVLGYSSQNSRATPDKNAVVEKET
                     AEKPAATQSPTAANAPAPAVATTPRESSSTTDNVMGARLIVGPDVKLKGAEILDCDTL
                     VVEGRVEATMDSRVIRIAENGSFAGTVSIDIAEIHGTFEGELTARSQLIIHSTGRVSG
                     TIRFGKLVVDEGGELCGEINVISGKAATQSKPAIEPYVALNAAAG"
     gene            3302..>3797
                     /locus_tag="PLD53_04795"
     CDS             3302..>3797
                     /locus_tag="PLD53_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC60259.1"
                     /translation="MIRISFRQSMLSGFLLITLLLSWAAVRNWLELERFVEQSRRGSE
                     LALQLSASIRELAERTVDLERSSRQFIVLNDSALLGRFDENVAHSLVVVERLESTPGR
                     PLGELPGVWRQAVEQLSQSLHQSTPQPELVPEVVSVLNRLSELNDELGLNARRWIDER
                     HATML"
     assembly_gap    3798..3897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3898..4129
                     /locus_tag="PLD53_04800"
     CDS             <3898..4129
                     /locus_tag="PLD53_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC60260.1"
                     /translation="IACIDKGPGIAAEDVERIFEPFVQGTRASPAPRKGSGVGLSIVR
                     ELMAAMGGRVLLAPCSGDATGANFQIEVPCER"
     gene            4148..4714
                     /locus_tag="PLD53_04805"
     CDS             4148..4714
                     /locus_tag="PLD53_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRC60261.1"
                     /translation="MKACRLREGFPVIVLLVSSLLVGCVTPPVPVDEKHAKHAKYATV
                     DESAMLPLLGYFQLLQRMSPQELARERIVLAAMPQTPVTLVRMAALLGQPRAPMDLAR
                     ALGLLESVLKSREPVAVSLLPLARTMTVQYQERLKLEQQNEKLLQQLKESQRRSGELQ
                     EKLDALADIERSLPARPTAGDTLPGATR"
     gene            4711..6117
                     /locus_tag="PLD53_04810"
     CDS             4711..6117
                     /locus_tag="PLD53_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRC60262.1"
                     /translation="MNPVSKPVAKSGSAHILVIDDDQDLLRLLSMRVHAWGYRVSTAV
                     SAEEGLARIAVEPPQLVLSDIRLPGKDGLALFDEIRSTRPTLPVILLTAHGTIPDAVQ
                     AMSRGVFGYLTKPFDSKVLHEKIQQALQLSQVSAGQETGNGETWREGIVFRSSRMAEL
                     MEEARVVAASDASVLIRGESGTGKEVLARAIHRASPRAKAPFVAINCSAIPEQLLESE
                     LFGHVRGAFTGAASNHVGLFQAADGGTLFLDEIGDMPLALQVKLLRVLQDRAVRAVGS
                     TRSEPVDVRVLSATHRDLEAAMSEGLFREDLYYRLDVVTMVLPCLDDRREDIPLLAAY
                     FVQQLVKKYDKGITGIAPEALEVLASASWPGNVRQLYNVVEQSCALATTPLITLTLVQ
                     RALRVPTMEALSYADAKQRFERNYLVQLLKLTDGSVADAARIADRNRTEFYRLLQKHE
                     LTPDMFRGASGNAGVADR"
     gene            complement(6218..6676)
                     /locus_tag="PLD53_04815"
     CDS             complement(6218..6676)
                     /locus_tag="PLD53_04815"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HRC60263.1"
                     /translation="MATHRETRAIARSPEYLYDIVADVERYPEFLPLVSAARIISRHE
                     DTYETEQSLALGLLTHRFRTRTKLDRPRAITVISEDRSFSRFHIRWNFSALDNDHCQV
                     DFELDCETRLLLLKPVIKVLIMPMATSMVSAFEARAHALARKAGLPDTRK"
     gene            7133..7690
                     /locus_tag="PLD53_04820"
     CDS             7133..7690
                     /locus_tag="PLD53_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HRC60264.1"
                     /translation="MNPHNEKKLHPLSWLIFSSRWMQLPLYLGLIFAQGVYVILFIKE
                     LFHLIAGATNLSEQLIMLAVLGLIDVVMISNLLVMVIVGGYETFVSRLNLKGHPDEPE
                     WLSHVNASVLKVKLAMAIIGISSIHLLKTFIAISTLGSENSNYTEAGAMWQTIIHMVF
                     ILSAMGIAYTDRLMSNALEDSKTRH"
     gene            complement(7720..8238)
                     /locus_tag="PLD53_04825"
     CDS             complement(7720..8238)
                     /locus_tag="PLD53_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60265.1"
                     /translation="MKHPHIPAWLRRFLDLSAEHRYYPLVVALIAFISTTTFSFPFVL
                     VLIPAVLVAPRRWLIIGLLCGLASGIGATVLVELFHHLGWEMVNQRYPDLLDSETLQH
                     VSEWLENYGLIALMIIAGSPMPQTPALLLYSLVNPSPPGVLFAVGIGKTIKYIFLAWA
                     TNRYPARFMEYR"
     gene            8486..8971
                     /locus_tag="PLD53_04830"
     CDS             8486..8971
                     /locus_tag="PLD53_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60266.1"
                     /translation="MMNKILAGLFSGLMAVFLPACDGINMAKLKPGISTVQDVREIMG
                     PPTMEWQDADGTLTWEYPRTPQGIVNYMIEFGPDKVLREIRQVLTEENFGKVHAGMSR
                     EDVRRLLGQPAHELYFPLKKEHVWDWKTKVDAGMDWYFNVHFDESWRVLRTSTNFVAG
                     G"
     gene            9269..>10431
                     /locus_tag="PLD53_04835"
     CDS             9269..>10431
                     /locus_tag="PLD53_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRC60267.1"
                     /translation="MQPDRTAELKALLEQRILILDGAMGTMIQRHGLKESDYRGTRFA
                     EHEHDLKGNNDLLLLTRPEIIRGIHAEYLAAGADIVETNTFNATAVSQADYKLESIVY
                     ELNFAGAKLAREVCDEFTTDDPAKPRFVAGVLGPTSRTASISPDVNDPGYRNTSFDQL
                     VDTYSEAIRGLCDGGADILLVETIFDTLNAKAALFSIEQHFEKAGRRWPVMISGTITD
                     ASGRTLSGQTAEAFWNSLRHIRPLSFGLNCALGAAELRQYVEELSHVCDCFISAHPNA
                     GLPNPLSPTGYDETPEQLAVEIVDWARHGFVNIVGGCCGTSPAHIAAIAQGVCSIAPR
                     VIPDIEKKLRLSGLEPFNVGKDSLFVNVGERTNVTGSKAFARMILEGRLDDALA"
BASE COUNT         2172 a         2812 c         3024 g         2323 t
ORIGIN      
        1 ccctcgacac gatcggcgcc tgccatcaag ccgagctcag tggcagccac ggcggtgccg
       61 cggtcattgt gcgggtgaag actgatgagg atgctgtcgc gacgcgcaag atggcgatgc
      121 atccattcga tctggtcagc gtaaatgttg ggcgtggctc tttccaccgt ggttggcaga
      181 ttgaggatga tcttgttgtc gggcgtggcc ccccaggcgt cggtaaccgc attgcatatc
      241 tcgagcgcga agtcgagctc ggtggcggta aatgtctctg ggctgtattc gagcacccat
      301 tccgtctccg gattctgctc ggcgagctgt ttgacgagct tgacggccgt tacggccatg
      361 gccaccacct cggctttgct catgttgaag acgatttcgc ggaacggctt cgaggtggca
      421 ttgtaaatgt ggacgatagc gcggcgagcg cctttgagcg actcgaaggt gcggcggata
      481 aactgttcgc gagccggggc aagcacctcg atggtcacgt catcgggaat atgcttttcc
      541 tcgatcagct tgcgaacgaa gtcgaaatcg gtctgcgatg ccgatgggaa cgccacctcg
      601 atttccttga aacccacttc gcatagcgtc ttgaacatgc gcattttgcg ttcggcgttc
      661 attggctcga agagcgcctg attgccgtca cgcaggtctg tgctcatcca gaccggtgcc
      721 gtagtgatgg tacggctcgg ccactggcga tcctcaatat cgaccggtgg aaaggcggtg
      781 tatttggtgg cgggttgttt caacatcatt tcgtactccc aaggtaggca tggaggccag
      841 tttcgatggg ggaatgatac tcaatgaagc gcagcaggtg cttgcgttat ttaagccgat
      901 caggcgcaaa tgaagcacaa aattgcgaat aaatataaaa acgtgtaatt tactggctca
      961 atggcaaaac atagggcaat atgatggaaa ttgaccgctt tgatcggcag attttgcaga
     1021 tacttcaggt tgatggacgt atcagcaatc aggacctcgc cgaccgtatt ggactttctc
     1081 cgtcaccatg tttaaggcgc gtacggaccc ttgaggactc cggtgtgatt acgggctatc
     1141 gtgccctgct caatgccaag gcgctcggtt attcattgat ggcgctgatc tacatctcca
     1201 tggacaagca cacccccgag cgcttcgaac acttcgagaa ggatatcagc cagatatctg
     1261 aggtgctcga atgcctgttg gttaccggtc aggacgcgga ttaccagatc aaggttgtcg
     1321 tcagggatat ggacgcttac caggagctcc tgctcaatcg cattacgcgc atacagggtg
     1381 tgaccggcgt gcattcgagt ttcgtgttgc gccgggtagt cgacaagact gcactacccg
     1441 tgtgatcggt tttggcggag gtgttcccgt ttgttcgagt tccggaggga gtaacggcct
     1501 tgctcagtcg gttgctgtgg ctaggtcgtc caaccgtcga tgtagcagaa ccgctggcgc
     1561 ttcaacgcgt tggcattgcc gcaactcgcc gatctggcgg aaacccagcg tacgctgata
     1621 gtaacgggca tggcgcggat tcacttcgat cacggcatcg ctggccgcga acatgtcctt
     1681 tccgtagttc cgggcaacct gaaacaaagc ggtcagcaag ccctgcgagc tgaaatccgg
     1741 atcaacagcc aacctagtaa cttcacatac tacccggcca ggtcgtcgca agctggcaag
     1801 ctccggtccg taaagcgaat ccgccagcaa gccatccggc gaatcacgac caagggtaag
     1861 ggttgccacc acttcgtcgt actgccaagc ggcaagagtt acccggttcg ggtcgtccgg
     1921 tcggagcccg accgcttcgg tgctataacc gcgccaagtg tacatttgct gtaccagcat
     1981 gctggccatg ccatgctggt gtggggttcg taccgggcga atgttgtaac cacggctacg
     2041 cggcaggagc ccccaaagtg gccgaagtga cgggagcagt tgatagcccc catcgaacgg
     2101 acgcgaagtc agcccatcca aagagaaagc catttctgga tacagactgg cgttgatggc
     2161 ggtttgcatg tggtaattcc gttcgggtta ttcaatagtc ctcaacctgc cgccgcgttg
     2221 agagcgacgt agggttcaat cgcaggcttg gactgggtgg ctgcctttcc ggaaataaca
     2281 ttgatttccc cacagagttc gccaccctcg tcgacaacca gcttgccaaa gcgaatcgtt
     2341 ccgctgaccc gccctgtcga atgaatgatc aattgggaac gagcggtcag ttcgccttcg
     2401 aatgtgccat gaatctcggc gatatcgata ctgaccgtac cggcgaaaga gccgttctcg
     2461 gcgatccgga tgacgcggct atccatggtt gcttccaccc ttccttcaac gaccaaggtg
     2521 tcgcagtcca gaatttccgc ccccttgagt ttgacgtcgg gacccacgat caatcgagcg
     2581 cccataacat tatctgttgt ggatgatgat tctctgggtg tggtggcgac agcgggggca
     2641 ggggcgttcg cggctgtcgg cgactgggtc gccgcaggtt tttcggcggt ctctttttcg
     2701 acaactgcgt tcttgtcggg agttgctctt gagttctgcg agctgtaacc gagtacgggt
     2761 tgggaatcac gacgggtgat gggctcgtta cggccaaaga cactgggttt gttgaacatc
     2821 atttttcctt ctcgctggtg tgaaatcctc gattggagga ccgtagcgag atatgtgcca
     2881 gatttttata actcatataa aacaataaga taccatgatt cattaaatta tagcgttaga
     2941 ttctactccg aacttgtcgg ggaatggcga caaatgtgcc gatcaatttg cgaaacgtct
     3001 gcatgcacag caacttgcgt tgtcgccaaa cagcgacaac gcatctaact acatgattaa
     3061 aagcgacaaa tatttctgcg aatgacttga ttcaggcttt cggcttgtct atttgtccgg
     3121 caaaatgtga gaaaggtggt ttaaactgcc cgccaatcat gtcgacaatc agaatcgccg
     3181 ttattcccgt gcgtgcgggg aaccagaagt gttcgactgg attccgggtc gggcatggaa
     3241 aaacgaaccc ggtcaatcgt tctcgcaatg actttgttcg ctttgtcccg acagggcaaa
     3301 tatgatacga atttcgttcc gccagagcat gctgtctgga ttcctgttaa ttacattgct
     3361 gctcagctgg gctgcggtgc gaaactggct tgagctcgaa cgctttgtcg aacagagccg
     3421 gcgaggcagc gaactggcgc ttcaacttag tgcctcgata cgcgaactcg cggagcggac
     3481 agtcgatctt gaacgcagtt cgcgtcagtt cattgtcctg aacgattcag cattgcttgg
     3541 gcgtttcgat gagaatgtgg cccactccct cgttgttgtg gagcgtttgg agtcgacccc
     3601 gggcaggcct ttgggcgaac tccccggagt ctggcgtcag gcggtcgaac agctcagtca
     3661 gagccttcat caatcaacac ctcagcctga gctggtccca gaagttgtat ccgttctgaa
     3721 caggctttcg gagctgaacg atgaattggg actcaatgcc cggcggtgga tcgacgaacg
     3781 gcatgcgacg atgctggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat
     3901 cgcttgcatt gacaaagggc cgggcattgc ggccgaggac gtggaacgca tcttcgagcc
     3961 atttgttcag ggcacacgcg cttcgcccgc gccgcggaag ggcagcggcg tgggcttgtc
     4021 gattgtccgc gaactgatgg cagccatggg cgggcgggtg ctgctggcgc cctgcagtgg
     4081 tgacgcaacc ggagcaaatt ttcagattga ggtgccctgt gaacggtagc tttgaaaagg
     4141 tgaatacatg aaggcctgtc gattgcgcga gggattcccg gtgatcgtgc tgcttgtctc
     4201 ctccttgctt gtcggctgcg tcacgcctcc ggtaccggtg gacgaaaagc acgccaagca
     4261 cgccaagtac gccactgttg acgagtcggc gatgctgccg ctgctgggtt atttccaact
     4321 gcttcaacgt atgtcgccgc aggagttggc gcgtgagcgg attgttctcg cggctatgcc
     4381 gcagaccccg gtaacccttg tccgtatggc ggcactgttg gggcaaccac gtgcgccgat
     4441 ggacctcgct cgggcacttg gcttgctgga aagcgtactc aaatccaggg agcctgtggc
     4501 ggtcagtttg cttcccctgg cccggacaat gacggttcag tatcaggagc gattgaaact
     4561 tgagcagcag aacgaaaaac ttcttcagca gttgaaggaa agccaacgcc gtagcggcga
     4621 gctccaggag aagctggatg ccctggccga tatcgaacgc tcgcttccag cgcgcccgac
     4681 agcgggcgat acccttcccg gagcaacgcg atgaatcccg tgtcgaaacc tgtagccaag
     4741 tccggctccg cccacatcct ggtcattgac gacgaccagg acttacttcg gctcctctcg
     4801 atgcgcgtac atgcctgggg ataccgtgta tctacggcag tctctgccga ggaaggcctg
     4861 gcgcggatcg ccgtcgagcc gccgcaactg gtgctcagcg atattcgcct gccgggcaag
     4921 gatgggctag ccctgtttga cgagattcgg tctacccggc ctacgctgcc ggtcatcctg
     4981 ctgaccgccc acggaacgat tcccgacgca gtgcaggcaa tgtcgcgggg agttttcggc
     5041 tatctgacca agcctttcga cagcaaggtg ctgcacgaga agattcagca ggccctgcag
     5101 ctctcccagg tcagtgctgg ccaggagacg ggtaatggcg agacctggcg cgagggtatt
     5161 gtcttccgta gcagccgcat ggccgaattg atggaagaag cccgtgtggt ggctgcttcg
     5221 gatgccagcg ttctcatcag aggtgagagc ggaacgggca aggaagtcct agcgcgggcc
     5281 atccaccggg ccagcccgcg cgccaaggcg cccttcgtgg ctatcaactg cagtgcgatt
     5341 ccggagcagc ttctggagtc cgaattgttc ggccacgtcc ggggtgcatt taccggcgct
     5401 gccagcaatc acgttggtct gtttcaggcc gccgacggcg ggaccttgtt ccttgacgaa
     5461 atcggcgata tgccactggc gctgcaggtc aagttgctgc gagtgctgca ggatcgggca
     5521 gtgcgggcgg taggatcaac ccgctcggag cccgtggacg ttcgggttct ttccgctacc
     5581 caccgtgacc tggaagctgc catgtcggaa ggacttttcc gcgaggattt atactatcga
     5641 ttggacgtcg tcaccatggt gctgccgtgt ctggacgacc ggcgcgagga cattccgctt
     5701 ctggcggctt acttcgtcca gcagctagtc aagaaatatg ataagggtat caccggaatt
     5761 gcaccggaag cccttgaagt actggcaagt gcgtcctggc caggcaatgt ccggcagctc
     5821 tacaatgtgg tggaacagtc ctgtgccctg gcgactacgc cgcttatcac cctgaccctt
     5881 gtccagcgtg ccctgcgggt gccgaccatg gaggcgctgt cctacgccga tgccaagcag
     5941 cgctttgaac gcaactatct ggtgcaactt ctcaaactga ccgatgggag cgtggcggac
     6001 gctgctcgaa tcgccgatcg caatcgaacc gagttctaca ggcttctgca gaaacacgaa
     6061 ctcacaccgg acatgtttcg aggcgcttcc ggcaacgccg gggtggctga ccggtagtca
     6121 agagcggtta ttaatcaggc cgttgcggag gctgctcaaa gcggtagcta gcgctggagc
     6181 gagtcacagt tgcggcaaca ggcctgtcgg cgcaggatta cttgcgcgta tccggcaagc
     6241 cggccttcct ggccagcgcg tgtgctctcg cctcgaaggc ggataccatg ctggttgcca
     6301 tcggcatgat aagaaccttg atgaccggtt tgaggagcag taagcgggtc tcgcagtcaa
     6361 gctcgaagtc gacctggcag tggtcattgt ccagagctga gaaattccat cgaatgtgaa
     6421 aacgtgagaa ggagcgatcc tcggaaatga cggtgatggc gcgtggccga tcgagcttcg
     6481 tccgtgtcct gaagcgatgc gtcagtaggc caagcgccag gctctgctcg gtctcgtagg
     6541 tgtcctcatg acggctgatg atcctcgctg cgctcaccag cggcaggaac tccggatagc
     6601 gctcgacatc tgcaacgata tcgtacaagt attcgggtga tcgagctatg gcgcgggttt
     6661 cgcgatgggt tgccatttag attgccttcc tgttgtctgc ccattgttgt tctgatggcc
     6721 cgagttctcg cggagaattg tcatcgcatt tcgcgtgttc gtcggttcat gatcgcaacc
     6781 ctgattctac cctgtgaacg tggttcgaca cttcgccgct gctccgaatg gtctgaaacc
     6841 gagggctctt gcaggactgc cccgagtatc ccggtgatcc gcactactgg ttgattccaa
     6901 agggcgtcac atgattattt cgtgctcgcc ttgagattcc gattcgtcat cttgtaaaat
     6961 gtgcgccggg cgaacctttg actcttgttt atcccgcaac aatccggcct tgagtccagt
     7021 gttttcagac tttcgacaca tcgacgccca gacgttctca caaccaatat ctcaccgcag
     7081 ctacgtttcg cactcacggt ggtgaaactg actgccagca atacaggtac ctatgaatcc
     7141 ccacaatgaa aaaaagctgc acccgctgtc ctggctgatt ttcagctcac gctggatgca
     7201 acttcccttg tacctgggcc tgattttcgc ccagggtgtc tatgtcatcc tcttcatcaa
     7261 ggaattgttc catctgattg ctggtgccac caacctgagt gagcaactca tcatgttggc
     7321 cgtattgggt ttgatcgacg tcgtgatgat ttccaacctg ctcgtcatgg tcatcgtcgg
     7381 ggggtatgag acttttgtgt cacggctgaa tttgaaagga catccggatg aaccggaatg
     7441 gctcagccac gtcaacgcct cggtgctgaa agtcaaattg gccatggcca tcatcggcat
     7501 cagctcaatt catctgttga agacattcat cgccataagc acgcttggat ctgaaaattc
     7561 caactacact gaggctggtg cgatgtggca aaccattatc catatggttt tcattctctc
     7621 ggcgatgggt attgcctata ccgatcgttt gatgtcgaat gcccttgagg attcaaaaac
     7681 aaggcattga tgttgtgaaa atgtatcgcc tgccagccat cagcggtatt ccataaagcg
     7741 agcgggataa cggttggttg cccaggccag aaagatgtac ttgatcgtct tgccgatacc
     7801 aactgcaaac agaacgccgg gtggcgacgg gttgacgagc gaatagagga gtagtgccgg
     7861 cgtctgcggc atgggtgatc cggcaatgat catcaaggca atgaggccat aattttccag
     7921 ccactcgctt acatgctgca aggtttcgga atcgagcaaa tccggatagc gttggttcac
     7981 catctcccag ccaaggtgat ggaatagttc aaccagcacg gtggccccaa taccgctggc
     8041 caggccacat aacaggccga taatcagcca gcgccggggg gcgacaagca cggcgggaat
     8101 cagtacgagt acgaacggaa acgaaaaggt cgtcgtggaa atgaaagcga tgagggctac
     8161 cactagcggg taataacggt gttcggcact caggtcgagg aatcgccgca accaggcggg
     8221 aatgtgaggg tgcttcatgg ctgatagaac cgtttccgtg attttgctgt gatcgctgca
     8281 atgcggtcag gtaaagattc gggatcgtct tgcgcttcgc gctgacaaag ggcaattcta
     8341 ccgaacgagt cattaatgta tgcatctcta ttcatacgta gtcaagacat cctctccacc
     8401 gaggctgttg cggtggcatc gcgcttagga ggctttcgat gatattcata aaatcttcgg
     8461 cacttttggc acgtcggcgg acgaaatgat gaacaagatt cttgctggcc tgttttcggg
     8521 actgatggct gtgtttctgc cggcttgcga tggcatcaac atggcgaaac tgaagcccgg
     8581 catttcgacg gtgcaggatg tgcgcgaaat catggggcca ccgacgatgg aatggcagga
     8641 tgccgacggc accctgacct gggagtatcc gcgtacgccc cagggaatcg tcaactacat
     8701 gatcgagttc ggcccggaca aggtgttgcg cgagattcgg caggtgctga ccgaggagaa
     8761 tttcggcaag gtccacgcag gcatgagcag ggaggatgtg cgccgtttgc tgggccaacc
     8821 ggcgcacgaa ctctacttcc cgctcaagaa ggagcacgtc tgggactgga aaacgaaagt
     8881 tgatgcgggt atggactggt atttcaacgt gcatttcgat gagtcgtggc gtgtgttgcg
     8941 gacgagcacc aatttcgtgg ccgggggctg acgtaacgct aggcaaggtg gatcaagcga
     9001 aacaaatcgt atggttccat cgtgtgctgt tgcctgtgtg agacgcgctg tggattgaca
     9061 atcaacggct tggcgcgtag atttgcgccc ttgcccgacg tcgttagcgg gttcgcgccg
     9121 atttgatctc attagattgc gggcgcgtta gaaatgccgc taaagcgtga ccggacacga
     9181 aacgaaaacc cgtcgagctt tgcgccgacg ggttttttgc attccgcagc gcactgatct
     9241 gacacccgac atcttttagg gaatcaccat gcagcccgac cgcacagccg aactgaaagc
     9301 cttgctcgaa caacgcattc tcatcctcga cggcgcgatg ggcacaatga ttcagcgcca
     9361 cggactgaag gagagcgatt atcgcggcac gcgcttcgcc gagcacgagc acgacctgaa
     9421 gggcaacaac gacctgttac tgctgacgcg acccgagatc attcgcggta ttcacgccga
     9481 gtatcttgcc gccggtgctg acatcgtcga aaccaatacc ttcaatgcta ctgcggtttc
     9541 gcaagccgac tacaagcttg aaagcattgt gtatgagctg aacttcgccg gcgcaaagct
     9601 cgccagagag gtgtgcgacg aattcaccac ggatgatcca gccaagccgc gctttgtcgc
     9661 tggcgtgctc ggcccgactt cgcgcacggc gtcgatctcg ccagacgtga atgatccggg
     9721 ctatcgcaac accagctttg atcaactggt cgacacctac agtgaagcca tccgcggcct
     9781 ttgcgatggc ggtgccgaca tcctgctggt cgaaacgatc ttcgacacgc tcaacgccaa
     9841 ggccgcgctg ttctcgatcg agcagcattt cgagaaggcc ggtcggcgtt ggccggtgat
     9901 gatttccgga acgatcaccg acgcctccgg ccgcacgctg tccggccaga cggccgaggc
     9961 cttctggaac tcgttgcgcc acatccggcc gctttccttc ggcctcaatt gcgcgctcgg
    10021 tgccgccgaa ctgcgccagt acgtcgagga gctgtcgcat gtctgtgact gtttcatctc
    10081 ggcgcacccg aatgccggac tgcccaatcc actgtcgcct accggttacg atgaaacgcc
    10141 cgaacaactg gcggtcgaaa tcgtcgactg ggcaagacat ggttttgtga acatcgttgg
    10201 cggttgctgt ggcacctcac ccgcgcacat tgcggcaatt gcacagggtg tatgcagcat
    10261 tgcgccgcgc gtcattcccg acatcgaaaa gaagctgcgt ctgtcagggc tggaaccatt
    10321 caatgtcggc aaggattcgc tctttgtcaa cgtcggtgaa cgcaccaacg tcaccggctc
    10381 gaaagccttc gcccgcatga tcctcgaagg tcgccttgac gatgcgctgg c
//
LOCUS       DAORPM010000066        10204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1509_length_10204_cov_9.277564, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000066 DAORPM010000000
VERSION     DAORPM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1509_length_10204_cov_9.277564"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="PLD53_04840"
     CDS             complement(<1..1447)
                     /locus_tag="PLD53_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC60268.1"
                     /translation="MSNLQLKIVEPELAVAEPANATPAAVTGTPILELKNLSKSYGTG
                     ANVTSVLKDINLQIKDGEFVAIVGFSGSGKTTLISLIAGLIAADSGAVLLNGQPVDGP
                     GADRGVVFQSYSLMPWMTVRQNVALAVDQVFRDSPKAEREARVEKYVAMVNLTPAMDK
                     RPAELSGGMRQRVSVARALAASPEILLLDEPLSALDALTRANLQDEIIRIWSQEKKTV
                     VLITNDVDEGIIMADRIIPLNPGPGATFGPEFEVTLPRPRDRTGINHSPEFKRIRKEV
                     TAYLMRVGSEQAAGGDAKVVALPGVRPNTSREHQASLKGVAMPIALPAHADRYVEFSQ
                     VSKIYPTKKGPVTVVEEFDLKIRQGEFISVIGHSGCGKSTVLSMVAGLSDISLGVIVL
                     DGREVANAGPDRGIVFQAPSLVPWLTAYENVALGVEQVYPHASRQERRDIVGHFLERV
                     GLADAADKKAAELSNGMKQRVGIARAFALSPK"
     gene            complement(1478..2497)
                     /locus_tag="PLD53_04845"
     CDS             complement(1478..2497)
                     /locus_tag="PLD53_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60269.1"
                     /translation="MINETASILTKTGLTWFVPFLRLAAGEDPRQQMKEILLQIGVPV
                     LAFALFLAVWNVLAAGVHTSLGTIPGPMQVVAEAKGLVEEHYSEREKAAGFYERQEAR
                     VAAALAEDPTAEVTMRTWTGKPTYLDQIITSLYTVFTGFALATLVAVPLGILCGLSPT
                     FQLAINPLIQIFRPVSPLAWLPIVTLVVSAVYVTSDGAWFEKSFITSAVTVTLCSLWP
                     TLINTALGVSSIDKDLINVAKVLRLPWYTKVVKIVLPSSLELIFTGLRLSLGVGWMVL
                     IAAEMLAQNPGLGKFVWDEFQNGSSHSLGRIMVAVFTIGFVGFLLDRVMYTLQQMVSF
                     SGQSH"
     gene            complement(2623..4032)
                     /locus_tag="PLD53_04850"
     CDS             complement(2623..4032)
                     /locus_tag="PLD53_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRC60270.1"
                     /translation="MKQYAMQNKWSLRAARFVRCALTSAVVATFVVGAPAMAAKAKIE
                     RPDLKFGFIKLTDMVPLAIAYEKGYFEDEGLFVTLEAQANWKVLLDRVISGELDGAHM
                     LAGQPIGATMGYGTKAHIITAFSMDLNGNAITVSNAVMDELKKKLPKQADGKPEHPIK
                     AEYLKAVVDDYKKQGKSFKMGMVFPVSTHNYELRYWLASGGIKPGYYAPLKGDTTGQI
                     NADVLLSVTPPPQMPSTMEAGTIDGYCVGEPWNQQAVFMGIGAPVITDYEIQKNNPEK
                     VFGVNKDWADKNPNTHKAVVKALIRAGYWLDAENNKNRAEAAKIIAKSNYVGADYKVI
                     ANSMTGTFEYEKGDVRKVPDFNVFFRYNATYPYYSDAIWYLTQMRRWGQIPEYKPDSW
                     YLDMAKKVYRPEVYASAAKELIAEGKMKASDFPDFDKETGFKPPAKDFLDGVVYDGTK
                     PNAYIDSLKIGLKGKETVK"
     gene            complement(4398..5180)
                     /locus_tag="PLD53_04855"
     CDS             complement(4398..5180)
                     /locus_tag="PLD53_04855"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRC60271.1"
                     /translation="MSSYCFSLRTPSLAIVMAALLAACAAPAPQTGIEWRPSPNFDAR
                     RPNLVVIHHTTDETAEEALGTLTNPERKVSSHYLVGRDGKVFQLVDEKDRAWHAGLSW
                     WNGQSDVNSASIGIELDNTGSEPFPDVQIDALLVLLAEIKERNGIPTANFVGHSDVAP
                     GRKVDPSDFFPWVALAAHGFGLWCAPPYPLAPDGFDSGLALAALGYNPSLPEASERAF
                     RLHYMHDVGDQSLSEADDIQMAIAARDLLFCLVQQKATLGSD"
     gene            complement(5286..6215)
                     /locus_tag="PLD53_04860"
     CDS             complement(5286..6215)
                     /locus_tag="PLD53_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRC60272.1"
                     /translation="MDSRLQRLPSLDPLKGFDAAARHLSFTKAAAELFLTQSAVSRQI
                     QTLEEQLGVRLFRRETRRLVLTPEGELLHRVVIEILSRLTEVCAGLRAGRRRPRVTVS
                     AAVGIAALWLVPRLSAFQEIEPDVDVRLSADNRIVDLDHEDIDLALRYVSPDNAPSGS
                     VLLFEEEVFPVAAPELAARLPATLRPEDLAQVSLLAFEHGHQAPWLSWDPWLAGMGLA
                     NAPPKAVMQFNQYDQLIRAAEDGRGIALGRGPLVAQMMSEGKLQLIGGTRLRVAARGY
                     YLVRTQPSARPEVERFATWLLGEAKQTERDAQA"
     gene            6335..6679
                     /locus_tag="PLD53_04865"
     CDS             6335..6679
                     /locus_tag="PLD53_04865"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HRC60273.1"
                     /translation="MAQQAQQTSYELAKRELLALDRAAGNRLHCTSGKIWITREGSQQ
                     DIILKPGESWLIGDDAAVVASALCASRLVVTHPQSTKPRVAPRQLAASILLLVRRWQH
                     RPLSGYPSTMLR"
     gene            6862..7125
                     /locus_tag="PLD53_04870"
     CDS             6862..7125
                     /locus_tag="PLD53_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60274.1"
                     /translation="MEDLALKRMQEGFMSGGVMTILIGGLALGGLLLYGSKSGQELPL
                     WPALAVCLVNLVAAIKIILDVKKAKKLRQETSSNKAKSIHPPR"
     gene            complement(7122..7910)
                     /locus_tag="PLD53_04875"
     CDS             complement(7122..7910)
                     /locus_tag="PLD53_04875"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRC60275.1"
                     /translation="MTHTIADLLQPLAQAREVVVFTGAGLSADSGIPTFRDGATGLWA
                     NIDPEEVVSIEGFERDPDKVWAWHESMRSLVADVCPNAGHDEIAELESLLPNARVTVV
                     TQNIDGLHQAAGSTRVLEIHGSALRVRCHDHCGFVEAWEAGQTPHRRCPSCGAPARPD
                     VVWFGEALDEEVFSLAAQATLDADVFFTVGTSAIIQPAASLAMAAKEYGALLIEVNPH
                     ETPFSEHADYSARIGASEFFPSLREALENFLEKTDGRDGNDHGR"
     gene            complement(8043..8615)
                     /locus_tag="PLD53_04880"
     CDS             complement(8043..8615)
                     /locus_tag="PLD53_04880"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRC60276.1"
                     /translation="MKKLIANEHEHLEETQIESTQVYRGKLLDVRLDRVRLPDGHESA
                     REYVIHQGAVVIIPVLDSGELIFERQYRYPLRRAFLELPAGKIEDGEDILKTATRELL
                     EETGHSASQWRYLGVVHPCIGYSNERIEIYIAQGLRRESDQQLDHGEFLDVLNLSLDE
                     ALDAIRRGDVTDGKTIAALFWAEKVLRSGW"
     gene            complement(8688..10160)
                     /gene="nuoN"
                     /locus_tag="PLD53_04885"
     CDS             complement(8688..10160)
                     /gene="nuoN"
                     /locus_tag="PLD53_04885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRC60277.1"
                     /translation="MQFVPPDLRLASAEIFLLAMACLILIVDLFVKDKKRTVTFFLTL
                     LALVGAAAATILTSSGEIVYTFSNMYVGDLMGDLLKLLLYVTVMVVLVYSRGYLQDRP
                     QMAKGEYYSLTLFATLGMMVMISANHFLTVYIGIELLSLSLYALVALNRDSIPATEAA
                     MKYFVLGALASGLLLYGMSMIYGATGSLEISAVSEHLFSGQVNKAILGFGLVFLVSGI
                     AFKLGVVPFHMWIPDVYHGAPTAVTLFIASAPKLAAFAMVIRLLVNGLLVAAQDWQTM
                     LLILSVLSMAVGNFAAIAQTNLKRMLAYSAISHMGFMLLGLVTGVVGGDARFALNAYS
                     SSMFYVITYVLTSAGTFGMILLLARAGFESDEISDFAGLNKRNPWYAALMMMMMFSMA
                     GIPFFVGFFAKFSVLQAVVAAGYLWLAIAAVFFSLVGAYYYLRVVKVMYFDPPANKSR
                     IEAPMDIKILLSANGLAVALIGVFPQTLMSLCAFSLLRSL"
BASE COUNT         2214 a         2988 c         2968 g         2034 t
ORIGIN      
        1 gcttcggcga aagggcgaag gcacgtgcaa tgccgacccg ctgcttcatg ccgttggaaa
       61 gctcggcggc tttcttgtcg gcagcatcgg ccaacccgac acgctcaaga aaatgcccga
      121 cgatgtcacg gcgctcctga cggctggcgt ggggatagac ctgttcgacc ccgagagcga
      181 cgttttcgta ggcggtaagc caaggaacca ggctcggcgc ctggaagaca atgccacgat
      241 cggggccggc attggccacc tcgcggccgt caagaacgat gactcccaga ctgatgtcgc
      301 tgaggccagc gaccatcgag agtaccgtcg acttgccgca accggagtgg ccgataaccg
      361 aaatgaactc gccttgcctg atcttcaggt cgaattcttc gacgacggtg acgggcccct
      421 tctttgtcgg atagatcttc gagacttgcg agaattcgac gtagcgatcg gcatgcgccg
      481 gaagcgcaat gggcattgca acgcctttaa ggctggcttg gtgctcacgg ctggtattgg
      541 gccgcacgcc cggcaacgcg acaacctttg catcgcctcc ggcagcctgc tcggagccga
      601 cccgcatcag gtaggcggta acttccttgc ggatgcgctt gaactcgggc gaatgattga
      661 tcccggtgcg atcgcgcggg cgcggcaggg taacttcgaa ctccggcccg aacgtcgcgc
      721 cggggccggg gttgagcggg atgatgcggt cggccatgat gatgccttcg tccacatcgt
      781 tggtgatgag cacgacggtc ttcttctcct ggctccagat gcggatgatt tcgtcctgta
      841 gattggctct ggtaagcgca tcgagcgccg aaagcggctc gtcgagcagc agtatctcgg
      901 ggctggcagc cagcgcacgg gcaacagaga cgcgctggcg catacccccc gacagttcgg
      961 cgggacgctt gtccatggcc ggggtgagat tcaccatggc gacgtatttt tcgacacggg
     1021 cctcgcgctc ggccttgggg gaatccctga acacctgatc aacggcgagc gcgacgttct
     1081 gccgcacggt catccagggc atcagcgaat agctttgaaa caccaccccg cgatccgcac
     1141 caggcccatc gactggctgg ccattgagga ggacggcgcc gctgtcggca gcgatcagcc
     1201 cggcgatcag cgaaatcagt gtcgttttcc cgcttccgga aaatccgacg atggcgacga
     1261 actcaccgtc cttgatctgc aggttgatgt ccttgagtac ggacgtgaca tttgcgccgg
     1321 ttccgtagga tttggacagg ttcttcagtt ccaggatcgg agtacccgtc acggcggccg
     1381 gggtcgcatt ggccggctcg gcgacagcca actcgggctc cacgattttc agttgcagat
     1441 tagacatggt gagtccttgt tgtgatggct gggttcttta gtgtgattgc ccgctgaaac
     1501 tgaccatttg ctgcagcgtg tacatcaccc gatccaacag gaagccgacg aaaccgatgg
     1561 tgaatacggc gaccatgatg cggccgagcg agtgtgaact gccgttctga aattcgtccc
     1621 agacgaactt gccaaggccg gggttctgcg ccagcatttc ggcggcaatc agcaccatcc
     1681 agcccacccc gagcgacagg cgcagacccg tgaagatcag ctcgagcgat gacggcaata
     1741 cgattttcac taccttggta taccacggga ggcgcagcac cttggcaacg ttgatcagat
     1801 ccttgtcgat cgacgaaacg cccagcgccg tattgatcag ggttggccac aaggagcaga
     1861 gcgtcacggt cactgccgaa gtgatgaacg acttttcgaa ccaggcaccg tcggatgtaa
     1921 cgtacaccgc gctgacgaca agggtgacga tcggcagcca ggcgagaggc gataccggcc
     1981 gaaagatctg gatcagcgga ttgatcgcga gctgaaaggt cggtgacaaa ccgcacagga
     2041 tgccaagcgg aacggccacc agcgtagcca gcgcaaaacc ggtgaaaacc gtgtacaggc
     2101 tggtaatgat ctggtcgaga tacgtcggtt tgccggtcca ggtgcgcatc gtgacttcgg
     2161 ccgtcgggtc ttcagccagg gccgcggcaa cgcgagcttc ctggcgctcg taaaaaccag
     2221 ctgccttttc gcgctcgctg tagtgttcct cgaccaatcc cttggcctcc gcaaccacct
     2281 gcatcgggcc gggaatggtt ccgaggctgg tatgcacacc ggcggcgagg acgttccaca
     2341 ctgccaggaa aagcgcaaat gcgagaaccg gaacgccgat ctgcaacaga atttccttca
     2401 tctgctggcg cgggtcttca cctgccgcca gtcgcaaaaa gggaacgaac caggtcaggc
     2461 ccgtcttggt cagaatactt gccgtttcgt taatcatgac agtggtcctc aaagtccaaa
     2521 agtttcgttc ctatcattcg gagttcacat cgccggggct gcgtcacagt ctcgcggtct
     2581 cgcagaccca cattcccggc gacagaagtc attccagcaa aactacttca ccgtttcctt
     2641 gcctttaagg ccgatcttca ggctatcgat gtaggcgttc ggctttgtgc cgtcgtacac
     2701 cacgccgtca agaaaatcct tcgctggcgg tttgaatccg gtttccttgt cgaagtcggg
     2761 gaagtcggac gccttcatct tgccttcggc gatcagctcc ttcgccgcgc tggcgtaaac
     2821 ctcggggcgg tagacttttt tcgccatatc gagataccag gaatccggtt tgtactcggg
     2881 aatctggccc cagcggcgca tctgcgtgag gtaccagatg gcatccgagt agtagggata
     2941 ggtggcgttg tagcggaaaa agacgttgaa gtcgggaacc ttgcgcacat cacccttctc
     3001 gtattcgaag gtgccggtca tgctgttggc aataaccttg tagtcggcgc cgacgtagtt
     3061 ggatttggcg attattttcg ccgcttccgc gcgattcttg ttgttctcgg catcaagcca
     3121 gtagccggca cgaatcagcg ccttgacaac ggccttgtgc gtgttcggat tcttgtcggc
     3181 ccagtccttg ttcaccccga agaccttctc cggattgttc ttctggatct cgtagtcggt
     3241 aatgaccggg gcgccaattc ccatgaacac ggcctgctgg ttccacggct cgccaacgca
     3301 gtagccatcg atggtgccgg cttccatggt gcttggcatc tgtggcggcg gagtgaccga
     3361 aagcagcacg tcggcattga tctgacccgt cgtgtcgccc ttcaacggtg cgtaataacc
     3421 gggtttgata ccgcccgaag ccagccagta acgtagctcg tagttgtggg tggataccgg
     3481 gaacaccatg cccatcttga agctcttgcc ctgcttcttg tagtcgtcca cgacggcctt
     3541 cagatactcg gccttgatcg ggtgctccgg cttgccgtcc gcctgcttcg gcagcttctt
     3601 cttcagttcg tccatgaccg cattcgaaac ggtgatggcg ttgccattca ggtccatgct
     3661 gaaggcggtg atgatgtgag ccttggtgcc gtaccccatg gtggcgccaa tcggctgtcc
     3721 agcgagcata tgggcaccat caagttcgcc cgagatgacg cggtcaagca gcaccttcca
     3781 gttggcctgg gcttccaggg tgacgaacaa accttcgtct tcgaaatagc ctttttcata
     3841 agcaatggcc agcggcacca tgtcggtcag cttgatgaag ccgaacttga gatccggccg
     3901 ctcgatcttc gccttggccg ccatggcggg tgcgccgacg acgaatgtcg caacgacggc
     3961 tgaggtcagc gcgcaacgga caaaccgtgc cgcccgcaat gaccatttgt tttgcatcgc
     4021 gtactgtttc atgaagttag ctcctgatga aaaaatcggc atccgagacg ctcttgcatc
     4081 tcgactcatg cctctcaaca gcagacttcg tgccagttaa cttttatcgt ttattttcag
     4141 cgcattacaa atagtttgta tttttgcgca gtacatgcag ctaccctgca tgcactatcc
     4201 gggtgcagat atcggtgaat ttccaacaat ggggagccgc actcacttgg ggacagcgtt
     4261 cgccagtgcg taatgagcga tcagggattg cggagtcatc aaaaaccgcg ggggcactcc
     4321 atcaacatat gatgttcaac cgggaccaat atttcgcgcg ctggcagttc gtgcgcgctg
     4381 gtgctcgatc gaaacgatca gtcgctgccg agagtcgctt tctgctgaac caggcagaag
     4441 agcaaatctc ttgcagcgat tgccatctga atgtcatcag cctcactcag actttgatcg
     4501 ccgacgtcgt gcatgtaatg caggcggaat gcccgttccg aagcttccgg caggctcgga
     4561 ttgtagccta acgcggccag cgcaaggcca gagtcgaaac cgtccggagc cagcggatag
     4621 ggtggcgcgc accacaggcc gaagccgtgc gcggcaagcg ccacccaggg gaagaagtcg
     4681 ctcgggtcga ccttgcgccc cggtgcaacg tccgagtggc cgacaaaatt ggcggtcggt
     4741 atgccgttgc gctctttgat ttcggcgagc agcacaagca gcgcgtcaat ctgcacatcg
     4801 gggaaaggtt cgctgccggt gttatcaagt tcgataccga tcgatgccga gttgacgtcg
     4861 ctctgcccgt tccaccatga cagtccggca tgccaggcac ggtctttttc gtcaacgagc
     4921 tgaaagacct tgccatcccg gccgaccaga tagtgcgaac tgaccttgcg ctccgggttg
     4981 gtcagcgtac ccaacgcttc ttcggctgtt tcgtcggtgg tgtgatggat gaccaccaga
     5041 tttggccggc gtgcgtcaaa gttgggcgac ggacgccatt cgatgccggt ctgcggtgcg
     5101 ggtgcagcgc aagcagcaag cagcgcggcc atgacgatgg ccagcgacgg agtgcgaaga
     5161 gaaaagcagt acgagctcaa gtcggcgaat ccaacaggtc gtaatcaggc gaccaatgcg
     5221 ctattttcgc cgcgaaggga tgtgatacgc aaattcccgt acccggcaga acatgaaaag
     5281 aacaatcagg cctgcgcatc gcgttcagtc tgcttcgctt cgcctaacag ccatgtggca
     5341 aaacgctcga cctcgggtcg cgccgatggc tgcgtgcgaa ccaggtagta gccgcgcgcg
     5401 gcgacccgca atcgcgtgcc gccgatcaat tgtagttttc cttcggacat catctgcgca
     5461 accagcggac cacgcccgag tgcgatgcca cggccatctt cggcggcccg aatcaactga
     5521 tcgtactgat tgaattgcat cacggccttc ggcggcgcat tcgccagacc cattccggcc
     5581 agccatggat cccatgagag ccaaggcgcc tgatggccgt gctcaaaggc gagcagcgat
     5641 acctgggcca gatcttcggg acgcagtgtt gcaggcagac gagcggccag ttcgggcgcc
     5701 gccacaggaa aaacctcctc ttcgaaaagc agcacagagc ccgatggtgc attgtcaggc
     5761 gatacatagc gcaaagcgag atcgatatcc tcgtggtcga ggtctacgat gcggttatcc
     5821 gccgagagcc gcacatcgac atcgggctcg atttcctgaa acgcagaaag acgcggcacc
     5881 agccacagcg cggcgattcc gacggcggca gaaacggtca cgcgcggtcg ccgccgacca
     5941 gcacgcaagc cggcgcagac ttcggtcagt cgcgaaagta tctcgatcac gacgcgatgc
     6001 agcagctcgc cttccggcgt caggaccagt cgccgcgttt cacggcgaaa cagccggacg
     6061 ccgagttgct cttccagcgt ctgtatctgg cggctgaccg cagactgggt aagaaacagc
     6121 tcagccgcgg ccttcgtaaa actcagatgg cgcgcggcgg cgtcgaaacc cttcagcgga
     6181 tcaagcgatg gcaagcgttg caagcggcta tccatgcgtt tttctcatgc gatatattcg
     6241 taatgatcgt ttgtcagtat gtagggtacg tcctatcttt gcaatcaatg cagaggcatt
     6301 ctgtctatgc ataaaacgca tgggggaaag gatcatggca caacaggcgc aacaaaccag
     6361 ttatgaactt gccaagcgcg aactgctggc cctggatcgg gccgctggaa atcgcctgca
     6421 ttgcacatcc gggaaaatct ggatcacacg agagggcagc cagcaagaca tcattctcaa
     6481 gccgggtgaa agctggctga tcggggatga cgccgcggtc gtcgcctcgg cactctgcgc
     6541 atcgaggctg gttgtcacgc atccgcagtc gaccaagcca agagtggcgc ctcgccagct
     6601 ggccgcttcg atcttgctgc tggtgcgacg ctggcagcac cgaccgcttt caggctatcc
     6661 ctccacgatg ttgcgctaag cgccattact tcgcgcagcg gaggagcaat ccgggacaat
     6721 gatcagcaaa gcatctttct gtaggggagt gacgccctgg ctggccttcg ccgcctcggg
     6781 ggtgtcaccg cgcgcggcta catggtaaat ttggcccttg ataccgggct ggcggactgt
     6841 cccgtctgcg gagtacatac catggaggat ttggctttga aacggatgca agaaggcttc
     6901 atgagcggcg gcgtaatgac tattctgatc ggcggcctcg cactcggcgg cctgcttcta
     6961 tacggctcca agtccggcca ggaattaccc ctctggccag ccctagccgt gtgcctggta
     7021 aatctggtgg ctgcgataaa gatcattctg gatgtgaaaa aggccaaaaa gctaaggcaa
     7081 gagacctcgt ctaacaaggc caaatcgatt cacccacccc gttagcgtcc atgatcattg
     7141 ccatcccttc cgtcagtttt ctccaggaaa ttttccagcg cttcccgaag agatggaaag
     7201 aactccgagg cgccgatgcg cgctgagtaa tccgcgtgct cagaaaatgg cgtttcatgg
     7261 ggattgactt cgatcaaaag agcgccgtac tccttcgctg ccatcgccag actcgccgca
     7321 ggctggatga tcgccgaggt acccacggtg aagaaaacgt cggcgtcaag cgtggcttgt
     7381 gccgccagtg agaacacctc ctcgtccaga gcttcgccga accagacgac atccggacgc
     7441 gccggagcac cgcaggaggg acagcgccga tgtggcgtct ggccggcctc ccatgcttcg
     7501 acaaatccgc aatgatcatg gcagcgaacg cgcagagcac tcccatgaat ttccaggacc
     7561 cgagtgctcc cggcggcctg atgcaggccg tcaatattct gggtgactac agtcacccgg
     7621 gcattcggca acaggctttc gagttccgcg atctcatcgt gtccggcatt gggacataca
     7681 tcggcgacaa gcgaccgcat tgactcgtgc caggcccaga ccttgtccgg atccctctcg
     7741 aagccctcga tgctcacgac ttcctcgggg tcaatgttgg cccacaggcc ggtggcgcca
     7801 tctctgaagg tcgggatgcc gctgtccgcc gagaggccgg cacccgtgaa aaccaccacc
     7861 tcccgcgctt gcgccagcgg ttgcaggagg tctgcaattg tatgcgtcat ggaatcagtc
     7921 tccttcggcc ggtattgtcc gtgctgcagg gataatgcta cacccacccg gaagaacgat
     7981 aagcgccatc cctatggagc ccgttcaggc gtagggcaga accggtattg cattgcggat
     8041 ccctaccagc cggaacgcag gactttttct gcccagaaga gagccgcgat ggtttttccg
     8101 tcggtgacat caccgcggcg aatcgcgtcc aacgcctcgt ccaggctcag gttcagcacg
     8161 tcaagaaact cgccgtgatc aagctgctgg tccgattcgc gtcgcaggcc ttgcgcgata
     8221 tagatctcga tgcgctcgtt ggaatagcca atgcagggat gcaccacccc caggtagcgc
     8281 cactggcttg cgctgtgccc cgtctcctca agcaattccc gcgttgcggt tttgaggatg
     8341 tcttcaccgt cctcaatctt cccggccggc aactcaagaa aggctctgcg cagcggatag
     8401 cgatactgcc gttcgaagat caactcgcca ctgtcgagca cgggaatgat gaccacagca
     8461 ccctgatgga tgacgtactc ccgtgcgctt tcgtgcccat ccggcaagcg cactcgatca
     8521 aggcgcacat cgagcagttt tccgcgatac acctgtgtgc tttcgatctg ggtctcttcc
     8581 aggtgttcgt gctcattggc gattagtttc ttcatccgta tccccacaaa taaaaacgcc
     8641 ccggccagcg gggcgtccat tgcatcaaag tttggtcagc gggctgacta cagcgaacgc
     8701 aacaaggaaa atgcacagag cgacatcaaa gtctgcggga aaactccgat cagggcgacg
     8761 gccaggccgt tggccgaaag cagaattttt atatccattg gcgcttcgat gcgcgacttg
     8821 tttgccgggg ggtcgaaata catgaccttg accacgcgca ggtaatagta ggcacccaca
     8881 agagagaaga acacggcggc gatggccagc cacagataac cggcggcaac gacggcctgc
     8941 agtacagaga acttggcaaa gaagccaacg aagaacggga tgccggccat cgagaacatc
     9001 atcatcatca tgagagctgc ataccacggg ttgcgcttgt tcaacccggc gaagtcgctg
     9061 atttcatcgg attcaaagcc ggcacgggca agcagcagaa tcattccgaa agtaccggcg
     9121 ctggtcagca cgtaggtaat gacatagaac attgacgagc tgtaggcatt gagggcgaag
     9181 cgcgcatcgc ctcccacgac accggtcacc aaaccaagca gcatgaagcc catgtgcgaa
     9241 atggccgaat aggcgagcat gcgtttgaga ttggtttgcg cgatggcggc aaaattgccg
     9301 acagccatcg atagaaccga gaggataagc agcatcgtct gccagtcctg cgcagcgaca
     9361 agcagcccgt tcaccagcag gcggatgacc atggcgaacg cagcgagctt tggtgcactg
     9421 gcgatgaaca gcgttaccgc cgtcggggcg ccgtggtaaa catccgggat ccacatatgg
     9481 aaaggaacga cacccagctt gaaggcgata ccggacacca ggaagacaag cccgaaaccg
     9541 aggattgcct tgttgacctg cccgctgaac agatgctcgg aaaccgcaga aatctccagc
     9601 gaccctgtgg cgccatagat catcgacatg ccgtagagca gcaagccgga agccagtgcg
     9661 cccaggacaa agtacttcat cgcggcctcg gttgcaggga ttgaatcgcg attgagcgcg
     9721 accaaagcgt agagcgacaa cgaaagcagt tcaataccga tatataccgt caggaaatga
     9781 ttcgctgaaa tcattaccat catgcccagc gtggcaaaca gggtcagcga gtagtactcg
     9841 cccttggcca tttgcggacg atcctggaga tagccgcgtg aatagaccag cacgaccatc
     9901 acggtcacgt aaagcaagag cttgagcagg tcgcccatca ggtcgccgac gtacatgttg
     9961 ctgaaggtat aaacgatctc tccggaactt gtcaggatcg tggcggctgc cgcaccgacc
    10021 agcgccagca gcgtaagaaa gaaagtcact gtgcgtttct tgtccttgac gaacagatcg
    10081 acgatcagaa tcaggcaagc catcgccagc aggaaaatct ccgcgctggc cagacgaagg
    10141 tcaggcggta caaattgcat ttcggcaagc gtcatagcgg ataccaatta taggatcttg
    10201 ctta
//
LOCUS       DAORPM010000067        10055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1537_length_10055_cov_11.509600, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000067 DAORPM010000000
VERSION     DAORPM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_10055_cov_11.509600"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1204)
                     /gene="sucC"
                     /locus_tag="PLD53_04890"
     CDS             complement(44..1204)
                     /gene="sucC"
                     /locus_tag="PLD53_04890"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRC60278.1"
                     /translation="MKIHEYQGKELLKKFGVVVPRGVFSSSVDDAVKAAESLGGKVWV
                     VKAQIHAGGRGKGGGVKVAKSLDEVRQYAGEILGMQLVTHQTGPTGQKVRRLLIEEGA
                     DIKKEYYVAALTDRVTQTVAMMASSEGGMNIEEVAHETPEKILKAFVDPAVGLTDAQA
                     TELAKGIGVPEASVGKAVAALKGLYTCYMETDASLAEINPLILEGNGDIKALDAKFNF
                     DSNALYRHPEIVAYRDLDEEDADEVEASKFDLTYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGAEPANFLDVGGGATTEKVTEAFKIMLKNPKVKGILVNIFGGIMRCDTIADGVVA
                     AARETHLTVPLVVRMKGTNEDIGKKILKDSGLPIITANSMAEAATKIVAAVK"
     gene            complement(1252..1803)
                     /locus_tag="PLD53_04895"
     CDS             complement(1252..1803)
                     /locus_tag="PLD53_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HRC60279.1"
                     /translation="MPLPTPSAKRVRMHQRSVKFEGFKRADGLWDIEARLVDTKDHDF
                     PLSSGLIRPGQAVHDMSVRITIDSKKTILEAATCADAAPYPGYCDTNPPDYSKIVGLN
                     LFKGFLKAVKDMFGMTRGCAHMSELLMFLPTVALQTLAGEAVDEHDSEHKPFQLDRCH
                     ALVSHSETVQRYYPRWYRNQNPG"
     gene            1950..3722
                     /locus_tag="PLD53_04900"
     CDS             1950..3722
                     /locus_tag="PLD53_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HRC60280.1"
                     /translation="MNNAAIRYSIRPLSVHAHTFEVRCTVASPDPTGQCFSLPAWIPG
                     SYLIRDFARHIVAIRAVSGSRPVSLTKLDKHTWQAARLKHGESITVTCEVYAWDLSVR
                     GAHLDETHAFFNGTSVFLRVLGQEELPCLIDIQPPAGEAFKDWRIATALEPAHGEQHC
                     AKRHGYGLYRAASYDELIDHPVEMGRFALARFVACGIPHEIAITGQHDCDMQRLTADL
                     KRICEWQIRFFGEPAPMPRYVFLITAVGDAYGGLEHRASTALLCSRNDLPWRGMKKMS
                     EGYRTFLGLCSHEYFHTWNVKRIRPAAFTPYDLDRENYTTLLWAFEGITSYYDDLALL
                     RCGAIELQDWLELLAKTISNVQRGPGRRRQTLAESSFDAWNKFYRPDENTPNAVVSYY
                     AKGALVALALDLTLRNKTNGAISLDDIMRALWQRHGKTGIGVGEEDIRLIAEECSGLN
                     LKRFFSAAVHGTGELPLKKLLATFGLKLEWVAAETPSFGVKTADEGGQLKLATVYDDS
                     AAQRAGLSAGDVLLAINDLRVASTNIDKLLTRCQPGDTVKVHAFRRDELKEFRVRLDR
                     SQDDMARLVADARPNNLRRRWLGI"
     gene            complement(3780..5114)
                     /locus_tag="PLD53_04905"
     CDS             complement(3780..5114)
                     /locus_tag="PLD53_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRC60281.1"
                     /translation="MFIHQSRLILLHAAPMLIAQLASMGMMVIDTVLLGHYGAEDLAA
                     VAVGGGIYIAVIFAFTGILQSVSPTVAHLKGAGRDDEIAGVLQQAFWLALLLAIPGVL
                     FLHFPDALLALSDIEPELEYKARGYLAMLAWGMPAALLYRTFYAFCNALGRPRPLMLI
                     SLGSTLLHGVLAWLLVTGALGGEGLGALGCGVSNALVGWFALFCSAAYMVFGKFLAPY
                     GLLGSWQRPRLGMLRELLRIGLPMGLSSFVEISAFTLIALFVAQMGATVVAGHRVVAN
                     LAAICYMLPLALSIATLAQVGQAAGARDWRRAEASISAGLLLAGGLSTILGLVLWWYA
                     RPLVEAYTSDPAVRLVALSLIGYVAIYQVFDALQTVAAYSLRGYKITFVPMLIHIACF
                     WVVGLFGGWWLAFRTASPMGVAGFWLGSLASLVLAAVLLGSVLWRAVRLVRD"
     gene            5300..6568
                     /locus_tag="PLD53_04910"
     CDS             5300..6568
                     /locus_tag="PLD53_04910"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRC60282.1"
                     /translation="MLDINRIVIQRLVLAWLIVSLLTGATIYIVESNRIDNAVIAFAS
                     TESQRFSPEGIDTKGMTPGELEALRQKALEFASKYFVVMKGYDYEGNKLFDVANPAYA
                     HIAALLPKLKFTFPRDGAHHYKKLAIDGETVVQIQVPLRSSTGSAAGYFEGVFVIPAE
                     ELARLDRDLHRLLLISLLCVLCTSLLLYPVIITLSRRVIRFSDQVTKGNLEIVTVLGA
                     AIAKRDSNTGSHNYRVTLYAIRLGEAVGTDKVDMRALILGAFLHDVGKIGIRDDILLK
                     PTELNDEEVAIMHTHVQLGIGIIQTSEWLHATHDVIKYHHEKYDGSGYMKGLRGEEIP
                     LEARIFAIVDVFDALTSSRPYKDAWPVDEALARLSKEAGCHFDPQLVSVFRNLAADLY
                     RQYGHASEADLHASLRQLVKDHYRQLLSQH"
     gene            complement(6594..7277)
                     /locus_tag="PLD53_04915"
     CDS             complement(6594..7277)
                     /locus_tag="PLD53_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRC60283.1"
                     /translation="MSYVFPPAPQVVLPVASSTDVFPVRRVYCVGRNYADHAAEMGAD
                     SREPPFFFSKPADALVPGGGEVTYPPMTDNLQHEVELVVALGKGGSNIPLDQALDCVF
                     GYAIGFDLTRRDLQSQAKDKGHPWDMGKGFDQGGPISALRPAGKVGHPEKGGIWLKVN
                     GESRQSGDLSQMTWKVAEVIANLSSYIALMPGDLIFTGTPAGVSTVVRGDVLEGGIEG
                     LGELKITLV"
     gene            complement(7298..8053)
                     /locus_tag="PLD53_04920"
     CDS             complement(7298..8053)
                     /locus_tag="PLD53_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRC60284.1"
                     /translation="MKREELTRYLDELLESGSFKDYCPNGLQVEGRAEVQRIVAGVTA
                     SQALVDAAIASAADAILVHHGWFWRGEDGRVTGFRKARMQALLMHDINLIAYHLPLDG
                     HVEFGNNAQLAKELGWTVEGRFGEQAIGWRGCLSAPTPLTELAQTITKVLRRAPQVIS
                     GRDDCDQPVCRIAWCSGGAQNLFEQAIDQGVDAYLSGEISEQNVHLARESGVAYIAAG
                     HHATERFGVQALAAHLGERFGLTCEFVDIDSPV"
     gene            8099..9274
                     /locus_tag="PLD53_04925"
     CDS             8099..9274
                     /locus_tag="PLD53_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRC60285.1"
                     /translation="MRRLWLIFAQTVTAGLAILFVISTLKPEWLGKPIHNGTSIVALK
                     ESSTGGEPLIGTGSYRDAARKALPSIVHIFTSQKIRMPRHPLIDDPIFRHFFGERPEA
                     DGQTKSGLGSGVIVSPSGYILTNFHVVDGADQIEVAMIDGRNLNARLVGGDPESDLAV
                     LQIAPEHSPEPKFPAITLGQMDTVGVGDVVLAIGNPFGVGQTVTMGIVSALGRSHLGI
                     NTFENFIQTDAAINPGNSGGALIDSQGNLIGINSAIYSRSGGSLGIGFAIPISTAKNV
                     MEQIIQTGTVTRGWIGVEVQQITQELAESFGLPSIEGALIAGVQRGSPADAGGIKPGD
                     ILLVVDGKSVKDPQAMLDLIAAQKPGNLVPFKLRRQKTLFDTSIRIGIRPQLKRERE"
     gene            complement(9280..10038)
                     /gene="tatC"
                     /locus_tag="PLD53_04930"
     CDS             complement(9280..10038)
                     /gene="tatC"
                     /locus_tag="PLD53_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRC60286.1"
                     /translation="MSPSEDSFLSHLFELRDRLIRVLLAVGLVFVCLFPWAKELYALL
                     AQPLLASLPQGGQMIATDVVGVFLVPMKVALMVAFLIALPYVLYQVWAFVAPGLYSHE
                     KRLALPLVAASVVLFFVGMSFAYFLVFPTVFGFMAKVAPEGVAWMTDIEKYLSFVMTT
                     FIAFGVTFEVPIVVIVLVRMRIVPLVTLKEWRPYVIVGAFVVAAVFTPPDVISQLMLA
                     IPLCLLFELGLLMARFISPPDVELSGSGQSSDKG"
BASE COUNT         2230 a         3104 c         2765 g         1956 t
ORIGIN      
        1 tgattttggt gtttttgttg atcagaatcg acatgttggc tccttacttg accgcggcaa
       61 cgatcttggt ggcggcttcg gccatggaat tggcagtgat gatcggcaga ccggagtcct
      121 tcaggatctt cttgccgata tcctcgttgg tgcccttcat gcgcacaacc agcggtaccg
      181 tcaaatgggt ttcacgggcg gcggcaacga cgccgtcggc gatggtgtcg catctcatga
      241 tgccgccaaa gatgttaacc aaaatgccct tgaccttcgg gttcttgagc atgatcttga
      301 atgcctcggt aaccttttcg gttgttgcac caccaccgac gtcgaggaag ttggcgggtt
      361 cggcaccgaa cagcttgatc gtgtccatgg tcgccatggc cagaccggca ccgttcacca
      421 gacagccgat attgccgtca agcgaaatgt aggtgaggtc gaacttggag gcctcgactt
      481 cgtcagcgtc ttcctcgtcg agatcgcggt acgccacgat ttccggatgg cggtaaagcg
      541 cattcgagtc aaaattgaat ttggcgtcaa gcgccttgat atcgccgttg ccttcgagaa
      601 tcagcggatt gatttccgcc agcgatgcat cggtttccat gtagcaggta tacagaccct
      661 tgagcgctgc tacggccttg cctacggagg cttccggaac gccgatgccc ttggccagtt
      721 cggtcgcctg cgcgtcggtc aggccgactg ccgggtcaac aaaggccttg agaatcttct
      781 cgggggtttc gtgcgccact tcctcgatgt tcatgccgcc ttccgaggac gccatcatgg
      841 ccaccgtctg ggtgacgcga tcggtcagcg cggcaacgta gtattccttc ttgatgtcgg
      901 cgccttcctc gatcagcagg cgacggacct tttgaccggt tggcccggtc tgatgcgtaa
      961 ccagctgcat gccgaggatc tcgccagcat attggcgcac ttcgtccagc gacttggcaa
     1021 ccttcacgcc gccgcccttg ccacgaccac cggcatgaat ctgggctttg acaacccaga
     1081 ctttgccgcc aagtgattca gccgctttta ccgcatcatc gacggaactg ctgaatacac
     1141 cacgcggaac caccacgccg aactttttga gaagttcctt cccctgatat tcgtgaattt
     1201 tcatgcgcct tccttgcttg aaattaagtg acgatcttta ggcaggatcc cctacccggg
     1261 gttctggttg cgataccagc gcgggtaata gcgctgaacc gtttccgaat gcgaaaccag
     1321 tgcgtgacat cgatcaagct gaaagggctt atgttcgctg tcgtgctcat caaccgcttc
     1381 accggccagc gtctgcaacg caacggttgg cagaaacatc agcagttcgc tcatgtgcgc
     1441 gcaacctcgc gtcataccga acatatcctt gacagctttc agaaaaccct tgaacagatt
     1501 caagccgaca atcttcgagt agtccggcgg gttggtgtcg caatagcccg ggtagggcgc
     1561 tgcatcggca cacgtcgccg cctctagaat tgtcttcttg ctgtcgatcg ttatccgcac
     1621 cgacatgtcg tgcaccgcct gacccggcct gatcagaccg gacgacagcg gaaaatcatg
     1681 atccttcgta tccaccaggc gcgcttcaat gtcccataag ccatccgcac gcttgaaacc
     1741 ctcgaacttg acgctacgct gatgcatgcg gacgcgtttt gcgctaggag taggaagtgg
     1801 cattgaaaat cccgggatgg caaatcagta cgttacgtac ctgaatgcat tggccgattc
     1861 tagcacaaaa gagaaggctg ttttgacgaa acctgttcgc tcagtattga cagtacaatc
     1921 cgccagtcat catccctgca catttgcgaa tgaacaacgc ggcgatccgg tattccattc
     1981 gtcccttgag cgtgcatgca cacaccttcg aggtgcgttg caccgtcgcg tcgcccgacc
     2041 cgaccggcca gtgcttctcc ctcccggcct ggattcccgg cagctacctg atacgcgact
     2101 ttgcgcggca cattgtcgcc atccgcgccg tgtcgggtag caggccggta agcctgacaa
     2161 aactggacaa acacacctgg caggctgcac ggctgaagca tggcgagtcg attaccgtca
     2221 cctgtgaggt ctatgcgtgg gacctttcgg ttcgcggcgc gcacctcgac gagacccatg
     2281 ccttttttaa cggcaccagc gtattcctgc gcgttcttgg gcaggaagaa ctcccctgcc
     2341 tgatcgacat ccagccaccg gcaggcgagg ccttcaagga ttggcgcata gccacggcat
     2401 tggaaccggc gcacggcgag cagcattgcg cgaagcggca tggttatggc ctttaccgtg
     2461 ccgccagtta cgacgaattg atcgaccatc cggtcgagat gggtcgcttt gctctcgcgc
     2521 gttttgttgc gtgtggaatc ccgcacgaga tagcgatcac cgggcaacac gattgcgaca
     2581 tgcagcgtct gaccgccgac ctcaaacgca tctgcgaatg gcagattcgc ttctttggtg
     2641 agccggcgcc gatgccccgc tacgtctttc tcatcaccgc agtcggcgac gcttatggcg
     2701 gtctggagca tcgtgcctcg accgccctgc tgtgctcgcg caacgacttg ccgtggcgcg
     2761 gcatgaagaa gatgagcgag ggctatcgca cgtttctcgg cctgtgcagc cacgaatatt
     2821 tccatacctg gaacgtaaag cgcatcagac ccgcggcctt cacgccatac gacctcgacc
     2881 gcgagaatta cacgaccttg ctctgggctt ttgagggcat aacgtcctac tacgacgatc
     2941 tggccctcct gcgttgcggc gccatcgagc tccaggactg gctcgaactc ctggcgaaaa
     3001 cgatcagcaa cgttcagcgc ggtcccggcc gccggcgaca gaccctggcc gaatcaagct
     3061 tcgatgcctg gaacaaattc tatcggccgg acgaaaatac cccgaatgcg gtcgtcagct
     3121 actacgccaa aggcgcgctg gtcgcgcttg ccctcgatct cacgctgcgc aacaagacca
     3181 acggggcaat atcgctcgat gacatcatgc gcgcgctctg gcaacgccac gggaaaaccg
     3241 gaattggcgt cggcgaggaa gatatccggc tgatcgccga agaatgctcc ggactcaatc
     3301 tgaagcgttt cttttcggct gcggtgcacg gcaccggtga attgccgctg aagaagcttc
     3361 tggcgacctt tggcctgaag ctcgagtggg tggcagcgga aacgccttcg tttggcgtaa
     3421 agaccgccga tgaagggggc cagctcaagc tggcaacggt atacgacgac agcgccgcac
     3481 agcgggccgg gctctccgcc ggcgacgtcc tgctggccat taatgacttg cgcgttgcgt
     3541 ccacgaacat cgacaagctc ctcacccgtt gccagcccgg cgataccgtc aaagtgcacg
     3601 ccttccggcg tgacgaactg aaggaattca gggtccggct cgatcgatct caagacgaca
     3661 tggccaggct ggtcgccgac gcgcgtccca ataacttacg gcggcgctgg ctgggaatct
     3721 gaacgacaag ccagcgaacg gtttccgata tcgcagccaa caccaacggc ctatcgacac
     3781 taatcacgca ccaagcgtac cgcccgccac agcacgctac cgagcaacac cgcagccagc
     3841 accagactgg ccaacgatcc aagccagaaa ccggcaacgc ccatcgggct ggccgtgcgg
     3901 aaagccagcc accagccgcc gaacaggcca accacccaga agcaggcgat gtgtatcagc
     3961 atcggcacaa aggtgatctt gtagccgcgt agcgaatagg ccgcaacggt ctgcagcgca
     4021 tcaaacacct ggtagatcgc cacgtagcca atcaacgaca gtgccacgag ccgaaccgcg
     4081 ggatcgctgg tataggcttc cacaagcggc cgggcgtacc accataagac aaggccaaga
     4141 attgtcgaca aaccgcctgc gagcagcagg ccggcgctga tcgatgcctc tgcccgccgc
     4201 cagtcgcgtg cccccgccgc ttggcctacc tgcgccagtg tggcaatgga caacgccaag
     4261 ggcagcatgt agcagatggc cgccagattg gcgacgaccc gatgcccggc aaccaccgtc
     4321 gcccccatct gcgcgacgaa aagtgcgatc aacgtgaagg ccgatatctc gacaaagctg
     4381 gacaaaccca tgggcagccc gatccgcagc aattctcgca acatcccgag gcgtggcctt
     4441 tgccaactgc cgagcagacc atagggcgcc agaaacttgc caaacaccat gtaagccgcg
     4501 ctgcagaaca gcgcgaacca tccgaccagg gcattcgaaa cgccgcagcc cagtgcgcca
     4561 aggccctcgc caccaagcgc tccggtcacc agcaaccagg ccagaacgcc atgcaacagg
     4621 gtgctgccca gtgagatcag catcagggga cgcggacggc caagcgcgtt gcaaaaagcg
     4681 taaaaggtgc gatagagcag cgcagcgggc attccccacg ccaacatggc cagatagccg
     4741 cgcgctttgt attcgagctc cggctcgata tccgacagtg ccagcaacgc gtccgggaaa
     4801 tgcaagaaca gcacacccgg aatggcgagc aacagcgcca gccagaaggc ttgttgcaag
     4861 acgccggcga tttcatcatc gcgccccgca cccttgagat gggcaaccgt cggcgatacc
     4921 gactgcagga tgccggtgaa tgcgaaaatc actgcaatgt atatcccgcc gccaacggcc
     4981 acagcggcaa gatcctcggc tccgtaatgc ccgagcaaca ccgtgtcgat caccatcatg
     5041 cccatcgaag cgagctgggc aatcagcatt ggcgctgcgt gcagcagaat cagccgggac
     5101 tggtggatga acattgacgg actcgttgta aacaggggtt cggacagcct attccatttc
     5161 tgctttgtac gaatgggtac gtacgaaatt gtcgtgtccc acgtttgcgc gagcgacttg
     5221 cgctggcttt cgtggcagta tacccggcga aaagttgggg ccggttgcac gccggcgctc
     5281 cttgaaacac ggaagccgca tgctcgatat caatcgcatt gtcatccaac gtcttgttct
     5341 ggcctggctg atcgtttcgc tcctgaccgg ggcaaccatc tacatcgtcg agtcgaatcg
     5401 aattgacaat gccgtcatcg cattcgcctc gaccgaatcg cagcgctttt cccctgaagg
     5461 catagacacc aaaggcatga caccgggcga gctggaggcc ttgcgacaaa aagcgctcga
     5521 atttgccagc aagtactttg tggtgatgaa aggctacgac tacgaaggca acaagctgtt
     5581 cgacgtcgcc aacccggcct acgcacacat cgcggcacta ctgccaaagt tgaaattcac
     5641 ctttccaaga gacggtgctc atcactacaa gaagctcgcg atcgatggtg aaacggtggt
     5701 gcagatacag gtgccgctga gaagctcgac aggcagcgcc gctggctact ttgagggggt
     5761 tttcgtgatc cccgccgaag aacttgcgcg ccttgatcgc gacttgcatc gactgctcct
     5821 gatttcgttg ttgtgcgtct tgtgtacgtc gctgcttctc tatcccgtga tcattactct
     5881 gagccgccga gtcatccgtt tttccgacca ggtaaccaag ggaaacctgg aaatcgtcac
     5941 cgtgctgggg gccgccattg ccaagcgcga ctcgaacacc ggcagccata actaccgcgt
     6001 gacgctttat gcgattcgcc tgggcgaagc tgtcggcacc gataaggtcg atatgcgtgc
     6061 gctcatcctt ggcgccttct tgcacgacgt cgggaaaatc ggcattcggg acgacatcct
     6121 gctcaaaccg acggaattga acgacgaaga ggtggcaatc atgcacaccc acgtgcaact
     6181 ggggatcggc atcatccaga cgtccgagtg gcttcacgcc acgcacgatg tcatcaagta
     6241 tcatcacgag aagtacgacg gtagcggcta catgaaaggg ctgcgcggcg aggaaatccc
     6301 ccttgaggcc cgcatcttcg ccattgtcga tgtattcgac gcgctgacct caagtcgtcc
     6361 ctacaaggac gcctggccgg ttgatgaggc gctggcccgg ctttcgaaag aggcaggctg
     6421 ccacttcgac ccgcaactgg tttcggtatt ccggaacctt gctgccgatc tatacagaca
     6481 gtacggccat gcgagtgaag ccgacctgca tgcgtccctg cggcagttgg tcaaagacca
     6541 ctatcggcag ctactctcgc aacactagcc ggtcctcttg caactagccg gcactaaacc
     6601 agcgtgatct tcagctcacc cagtccttcg ataccgccct caagcacgtc accgcgcact
     6661 acggtcgaaa cgcctgccgg tgtcccggtg aatatcagat cgcccggcat gagagctata
     6721 tagctggaga gattggcaat cacttcagcg accttccagg tcatctgtga caagtcgccg
     6781 ctctgccttg attcgccgtt gaccttgagc cagatgcccc ccttttccgg atgacccacc
     6841 ttgccggcgg gcctaagagc gctgatcgga ccgccttgat caaacccctt gcccatgtcc
     6901 cagggatgcc ctttgtcctt ggcttgagac tgaagatccc gccgggtcag atcaaaaccg
     6961 atggcgtagc caaagacgca atcaagagcc tggtcgagcg ggatattgga gccgccttta
     7021 cctagcgcga cgaccagctc aacctcatgc tgaagattgt cggtcatcgg tggataggtt
     7081 acctcgccac caccgggaac cagcgcatcc gctggtttgc taaagaagaa cggcggctcg
     7141 cggctgtcgg cgcccatttc ggccgcatgg tccgcgtaat tgcgcccgac gcaatagacg
     7201 cgacgcaccg gaaaaacatc ggtgctgcta gccaccggaa gaacgacctg cggcgcaggc
     7261 ggaaatacat agctcatgag tgctccctaa gaaatattca aaccggactg tcgatatcga
     7321 cgaactcgca tgtcaggcca aagcgttctc ccaggtgtgc cgccagggcc tgcacgccaa
     7381 aacgctccgt ggcatggtgt ccggcagcga tgtaggcgac gcccgactca cgcgcgaggt
     7441 gcacattctg ctcggaaatt tcaccggaca gatacgcatc gacgccctgg tcaatcgcct
     7501 gctcgaaaag attttgcgcc cctcccgaac accaggcgat gcggcacacc ggctgatcgc
     7561 agtcatccct gccggaaatc acttgaggcg cacggcgcaa taccttggtg atggtttgcg
     7621 ccaattccgt taacggcgtc ggcgccgaca ggcagccgcg ccagccaata gcctgctcgc
     7681 cgaagcgccc ttcgacagtc cagccaagtt cttttgccag ttgcgcattg ttgccgaatt
     7741 cgacatggcc atccagcggc aagtggtaag caatcaagtt gatgtcgtgc atcagcagcg
     7801 cctgcatgcg cgccttgcga aaaccggtca cgcggccatc ttcgccccgc cagaaccagc
     7861 catgatgcac gagaatggcg tcagccgcgg atgcaatcgc cgcatcgacc agtgcctgac
     7921 tggcagtaac accggcaaca atgcgctgca cctcggcacg accttccact tgcaggccgt
     7981 ttgggcaata atccttgaaa cttcccgatt ccagcagttc gtccagatag cgtgtcaatt
     8041 cttcacgttt catccgaaac ttcctttctc cgttgtcttc tttcagatcc gatttcccat
     8101 gcgcagactc tggctgattt ttgcacaaac cgttacggca ggtcttgcca ttctctttgt
     8161 catcagcacg ctcaagcccg aatggctggg caaaccaatc cacaacggga ccagcattgt
     8221 cgccttgaag gaatcgtcaa ccggaggcga accgctcatc ggcacgggtt cctaccgtga
     8281 tgccgccagg aaagcgctgc cttccatcgt acatatcttc acctcgcaga agatcagaat
     8341 gccgcgccac ccactgatcg acgacccgat tttccggcac ttttttggcg aacgcccgga
     8401 agccgacggt caaaccaaat cggggctggg ctcgggtgtc attgtcagcc ccagtggtta
     8461 cattctcaca aatttccatg tcgtcgatgg agcggaccag attgaagtgg ccatgatcga
     8521 cgggcgaaat ctgaatgccc gcctggtcgg cggcgacccc gagtcggatc tcgccgtctt
     8581 gcagatcgca ccggaacatt caccagagcc caagttcccg gccatcaccc tcggccagat
     8641 ggataccgtt ggcgtcggcg acgtcgttct tgctattggc aatcccttcg gggttgggca
     8701 gacggtaacg atggggattg tctcggcact cggacgttcg cacttgggca tcaatacctt
     8761 cgagaacttc atccagacgg atgcggcgat caacccagga aattcgggtg gtgccctgat
     8821 cgatagccag gggaatttga ttggcatcaa ctctgcgatc tactcgcgct ctggcggctc
     8881 gctcggcatc ggtttcgcca tcccgatatc taccgccaaa aacgtaatgg agcaaatcat
     8941 ccagaccgga accgtgacgc gcggctggat tggtgtcgaa gtgcagcaga tcactcagga
     9001 actcgccgag tcctttggtc tccccagcat tgaaggcgca cttatcgctg gtgttcagcg
     9061 tggcagtccc gcggacgcag gcggaatcaa gccgggcgat atcctgttgg tggtggatgg
     9121 aaagtccgtc aaggatccgc aggccatgct tgacctgata gccgcgcaaa aaccaggcaa
     9181 tctggtgccg ttcaaactac gtcgtcagaa aacgcttttc gacacgtcca tccgtatcgg
     9241 aatacggccg caactcaagc gtgagcgcga atgaacacgt cagcctttgt ccgaactctg
     9301 cccagagcct gacaattcaa cgtccggcgg gctgatgaaa cgcgccatca gcaacccaag
     9361 ctcaaagagc agacacagcg gaatggcgag cataagctgc gaaatcacgt cgggcggcgt
     9421 aaagacggca gcgaccacga atgctccgac aatcacatac ggacgccatt cctttagggt
     9481 gaccagtggc acgatgcgca tccggacgag gacgatgact actatcggca cctcgaaagt
     9541 gacgccgaac gcaataaagg tagtcatcac gaaggacaga tacttttcga tatccgtcat
     9601 ccaggcgacg ccctccggcg cgaccttggc catgaagcca aacaccgtcg gaaacaccag
     9661 gaagtacgca aatgacattc cgacgaagaa caacacgacg ctcgctgcga caagcggcaa
     9721 cgccagcctt ttctcgtgag aatacaggcc gggcgcaacg aacgcccaga cttgatagag
     9781 aacgtaaggc aaggcgatga gaaaggcgac catcagagcc accttcatcg gtaccagaaa
     9841 gacgccgacg acgtcggtcg ctatcatctg cccgccttgc ggcaacgatg ccagaagcgg
     9901 ctgcgcgaga agcgcataca gctccttcgc ccacgggaac aggcaaacaa aaacgagccc
     9961 gacagcgagc agcacccgaa tcaggcggtc gcgcaactcg aagagatgtg agagaaagga
    10021 gtcttccgac gggctcatgc gtcgttcacg ttttc
//
LOCUS       DAORPM010000068        10046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1539_length_10046_cov_11.080072, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000068 DAORPM010000000
VERSION     DAORPM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1539_length_10046_cov_11.080072"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..1349)
                     /locus_tag="PLD53_04935"
     CDS             complement(309..1349)
                     /locus_tag="PLD53_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRC60287.1"
                     /translation="MTEGNHRVITVGIDLAKNVFAVHGVDDNGKSVLVKPKVSREHLL
                     PLIAPLPPCVIGMEACSGAHHWARLFRQHGHTVRLMAPKFVTPYRLSGKRGKNDAADA
                     QAICEAVRRPHMRFVPVKEEHQQIILCLHRTRNGFVEERTAIYNRLRGLMSEFGIVLP
                     QKVACLRRSVGAHLEDLPGCANRCIGDLLAHADALDARIAEYDRLIAQTAREDARSKR
                     LMQRPGIGPVTASALLASIGNGHDFKNGRQVAAWIGLVPGQYSSGGKSRLGRITKAGD
                     AYLRSLLVMGARAILSGLDGKQDGFSRWARRLVERRGYWKAVVAIAAKNARLAWAVLH
                     YGEDFRLSQSTA"
     gene            1913..2215
                     /locus_tag="PLD53_04940"
     CDS             1913..2215
                     /locus_tag="PLD53_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRC60288.1"
                     /translation="MDTKTGLSGHDHHHRAENSHATHGMHHDHEAANTTTVKDPVCGM
                     NVTLGAGKPSFEHDSKTWHFCSQKCHDKFAADPVHYLTGEHKKAAAQATAPASAGT"
     gene            2228..2572
                     /locus_tag="PLD53_04945"
     CDS             2228..2572
                     /locus_tag="PLD53_04945"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRC60289.1"
                     /translation="MHSETIRDAPGDCPKCGMALESMGVTSGDEGFNPELVDFRRRFL
                     IGAVLTVPLLVLTVGPFLGLGFVRDLLGERTAQWVKLALGTSASHWRPARPGMSGSGN
                     ACSRLEIKTSSR"
     gene            2609..3178
                     /locus_tag="PLD53_04950"
     CDS             2609..3178
                     /locus_tag="PLD53_04950"
                     /inference="COORDINATES: protein motif:HMM:NF009166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRC60290.1"
                     /translation="MEESRTDETLMLAYRDGDAAAFEVLYARHRGRLFRYLLHQCARR
                     EQADEMFQEVWMSVIRARSGYEVSAKFTTWLFRIAHNRLIDGYRARGRLAEFEADATD
                     FEGDPPDHPAPTSGQPEQLYERALVAGNMVAAIEALPAPQREAFLLAAEGGLTVEEIG
                     NATGTGYETAKSRLRYAYARLRSELRDLR"
     gene            3175..4104
                     /locus_tag="PLD53_04955"
     CDS             3175..4104
                     /locus_tag="PLD53_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60291.1"
                     /translation="MTRPIIPPKDPIPDSTVSEIHALYREGAGAAEPGPSLDRAILDA
                     ARADLRADNGAKQRRQLPWWKRWIPATTAIAVAVIGLSFTLRVSELQESDLSAVTGAM
                     EPKRDDAKIAADKVPGKATLSDRPAKSQAASKAEVPKPNQNDESKSGQNFSSAVREMT
                     ARPAPAAERRAIPARQRPSPDGAAQAPATMAQPVPASEPQKKSLRSDGYGRLEKSESD
                     ASGAAGAELARPPLRREAEHIGVSSRDGPAADSIAPSLSTRPDDATTPEAWLELIRKH
                     RAAGRNAEAAQSLARFRMRYPDYVLPADLIQPK"
     gene            4201..5109
                     /locus_tag="PLD53_04960"
     CDS             4201..5109
                     /locus_tag="PLD53_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60292.1"
                     /translation="MNKTNFASLVLGAIGFLALTGCAQPSLAARLVDVSVVNRSTGER
                     LTPYRHHGKFYIAGTPGDNYSIELRSRRSERLLTVLSVDGVNVLTGETAAPLQSGYVL
                     DDRQSYGISGWRKSMNDVAQFVFTALPNSYAARTGRPDNVGVIGVAVFREKVVPPLAR
                     YGTTAPFPATKEARHPEPARDADEGPVRQAAKSESEASTGASKSTASGKLAERRANLA
                     AADSASPAEFEKKAERLGTGHGEREYSPTRYTGFERARDTPDEIVTIYYDSRDNLIAR
                     GIIPTPWLAEPTPFPANARFVPDPRS"
     gene            5306..5761
                     /locus_tag="PLD53_04965"
     CDS             5306..5761
                     /locus_tag="PLD53_04965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRC60293.1"
                     /translation="MDESRKNCELCASPGGELVWKSALCRVVLIDDTDYPGFCRVILN
                     RHEPEMTDLAVNERQAMMRVVFAVESALRHLINPDKINLASLGNVTPHVHWHVIPRWR
                     EDRCFPNPIWGLAQRTDVALPAKIDLSILRKALAEQLGEPIVSRERSFP"
     gene            5758..7359
                     /locus_tag="PLD53_04970"
     CDS             5758..7359
                     /locus_tag="PLD53_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60294.1"
                     /translation="MNAMSPITFAIHDLQTGCDIISRIPLANPQQAAIDLDRFLDSLL
                     TSPPDGETYFQLLEHARVPISFVADELAKRYQSKALPLAEVEEETFEQVIALWRKAAT
                     SYARCAENDASDEQDPASASRVATILHRCIHHSGMIILEHQRARRELPWGLWLELHGY
                     YGSAEEWGVATLAVPDALDPLGRSTDCTTAYIAFLMCDMAGSFSLSVRDQTLVRRWAS
                     HWSPLVSLHKAEPGEALPQYVVDLMQDMALRPVAECLQTEHLRRLDTTRLTMHINQVR
                     HQLSQKIPPSHLELGEDCTAGQCKRLLEHLAPAWAQARAARKYRRHATSGITQLCTGF
                     EEMHYFISDKEFEQPENVRVYSRKEFENLFAFRHQTDPQQALHVRQEQLTYGVDSWEV
                     VNQSANGFRLVRSTSGKKMIHGQLIALRPKDGQRFLLAKNTWLMQEKSGGLIAGIKAL
                     PGLPVATAARVIEQASESHGQYHRAFLLPAVPSQDTDQSLVLPQGWFRNGRVVELFTD
                     GAWRVKLQQVLDDGPGFERVSFVVC"
     gene            complement(7369..8670)
                     /gene="phoR"
                     /locus_tag="PLD53_04975"
     CDS             complement(7369..8670)
                     /gene="phoR"
                     /locus_tag="PLD53_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HRC60295.1"
                     /translation="MSAIWVRLIWQLLLFTCIVFPPVFYIYGLNWGLASAVIFLLLLL
                     CWHLFHLQKLMSWLEGELNSPLPEGRGVWETTFAELHRRVRMRLGQQQLLAETLENFM
                     SVFQALPDGVIAFDRHRRIDWINERAETHFALSAASDRGLVLTNLIRHPDFVAYLDSG
                     QYAEPLIYRDGRVDGMTLLLQIIPYGEDQNLLVSRDVSQVERVETIRRDFIANVSHEL
                     KTPLTVVAGFSEMLVGGHEAYGGAKINNYLTLICEQTARMQRLIEDLLTLSSLESGGP
                     WLNEERVELEPMLRSILADAQALSAGKHEIVLNVETPSVLAGYASELRSAFSNLAGNA
                     VRYTPPGGRIDLLWRVHDGFGEFVVTDTGIGIAAQHLPRLTERFYRVDRSRSRETGGT
                     GLGLAIVKHVLTRHHATLEIESTLGKGSRFSARFPERCILG"
     gene            complement(8699..9391)
                     /gene="phoB"
                     /locus_tag="PLD53_04980"
     CDS             complement(8699..9391)
                     /gene="phoB"
                     /locus_tag="PLD53_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HRC60296.1"
                     /translation="MSATILVVEDEPSIQELIAASLQHAGHRVMRADTAEDAIRLVNS
                     TLPDVVLIDWMLPGMSGVQYARRLRSEERTRYLPIIILTARSEEHDKVAGLEAGADDY
                     LTKPFSPRELLARIKAVLRRRAPQMTEDCVDVEGLRVDPVSHRVSGNGQSLDLGPTEF
                     RLLHFFMTHAERVHSRTQLLDQVWGDHVFVEERTVDVHIRRLRSSLEQTGHDRLIQTV
                     RGSGYRFSALPE"
     gene            9608..10039
                     /locus_tag="PLD53_04985"
     CDS             9608..10039
                     /locus_tag="PLD53_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRC60297.1"
                     /translation="MAGLNPGTPIPVEIKLHNKSRIVELAFENDERYELTYEFLRVFT
                     PSAEARGHGAGQETLQVGKRDVEIVTIEPVGNYAIKPVFSDGHESGLYSWDILYTLCA
                     NRDALWQAYLDKLKAEGGSRDPSECRPDTAKPPPSCGSGKR"
BASE COUNT         2150 a         3012 c         2848 g         2036 t
ORIGIN      
        1 tcagttcatc gtttttcctg atctgatttc aatttcactg cgcatgctga acaagaagat
       61 ggcggactgc gaaactacaa ccggtcattc tggacaaatt gtccggaccc tgatgaaaga
      121 cgcgcgccgg ggcctcaatc gttagtcggg aatgacttct tccccttgag ctaatcaggc
      181 tctcccggcg cgggtccaac ccgcttcaca tcaacgcatg cagtgcactg gtgattcctg
      241 gccgccctct ggcggcacgt ctcatccttt cagttctctc tttctctata gacgcttttg
      301 tcggggcttc aggccgtgct ctggctgagc cggaaatcct cgccgtagtg aagtaccgcc
      361 caggccagcc gcgcattctt ggcggcaatg gccaccaccg ccttccagta gccccggcgt
      421 tcgaccaaac ggcgtgccca gcggctaaaa ccatcctgct tgccgtccaa cccggagaga
      481 atggcgcggg cgcccatgac cagcagactt ctgagataag cgtcacccgc cttggtgatg
      541 cgccccagcc gggacttgcc gccactgctg tattgtcccg gcaccagtcc gatccatgcg
      601 gcgacctggc ggccgttctt gaagtcgtgt ccgttgccaa tgctggccag gagtgcgctg
      661 gcggtgacgg ggccgatacc ggggcgttgc atcaggcgct tgctgcgtgc gtcctcgcga
      721 gcggtctggg cgatcagccg gtcgtactcc gcaatcctgg catcgagcgc atcagcgtgg
      781 gcgagcagat ctccgataca gcgattggca caccccggca ggtcttcgag atgagcgcca
      841 acgcttcggc gcagacaggc gaccttctgc ggcagaacga tgccgaattc ggacatcagg
      901 ccgcgcaggc ggttgtagat cgcggtgcgt tcttcgacga agccattgcg ggtgcggtgc
      961 aggcacagga tgatctgttg gtgttcttcc ttgaccggga cgaagcgcat gtggggccgg
     1021 cggacggctt cgcagattgc ctgcgcgtcg gccgcgtcat tcttgccgcg cttgcccgac
     1081 aggcggtagg gcgtgacgaa tttgggggcc atcagccgaa ccgtgtgtcc gtgctgacga
     1141 aacagccgcg cccagtggtg tgcgccggag caggcttcca tgccgatgac gcagggtggc
     1201 agtggggcga tcagtggcaa aagatgctca cgtgatactt tgggcttgac cagcacggac
     1261 ttcccgttat catcaacgcc gtgtacggca aagacgttct tggccagatc gattcctacg
     1321 gtgatcacac ggtgatttcc ttcggtcata atgctcatgg acttcccctt tcgtggtgtt
     1381 gatgttgtct tgaaactcca tcatggcacg cgttgccgat tgcggcttcc gccgctacct
     1441 cgggacgggg aagtcccttt cattcgttaa gtagacacca ttccgtccag tcgtcagatt
     1501 attggattgc ccaataatct ggcttcaggt cgcatggtgc tgtttatcag tgaagcattt
     1561 ctctgaattg agtccgtcat gccggatatt ctgtttacag tttctgcttc cttgggttgt
     1621 tcaaaactgg catgtctatg cttgtaaggg ctgcttcatg gaacagtcgc caccgaccgg
     1681 ttgtggctgt tgctaccaga tgacgattaa atctcatcac tgaaacctgg tcaacaaaat
     1741 cgcccgcgga atttgcgcgt cgcagaacaa acagccccga caggctttat ctattgcaat
     1801 cacccggcgg cgaagtgcat cggctggcca tcgtgccctg aagaaagggg gcttgacctg
     1861 tgagcgacac gcttgtctaa gctgaaagca gtaccaattg gagaggactg ccatggacac
     1921 gaaaacaggg ttgagcgggc acgaccatca tcaccgggcc gagaattctc acgccacgca
     1981 cggcatgcac catgaccatg aagcggccaa cacaacgacc gtgaaggatc cggtctgcgg
     2041 catgaacgtc acccttggcg cgggcaagcc gagcttcgaa cacgacagca agacctggca
     2101 cttttgctcg cagaagtgcc acgacaagtt cgccgcggac cccgtgcact acctgacagg
     2161 cgagcacaag aaggccgccg ctcaggctac cgctccagca tctgcaggaa cgtaatacac
     2221 ctgcccgatg cattccgaaa ccatccgcga cgcgccgggc gattgcccga aatgcggcat
     2281 ggctctggaa tccatgggcg ttacgagcgg cgacgaaggg tttaaccccg agttggtcga
     2341 cttcaggcgc cggttcttga tcggtgccgt tttgaccgta cctctgctgg tgctgaccgt
     2401 ggggccgttt ctcggtctgg gcttcgtacg tgatctgctc ggtgagcgca cggcgcagtg
     2461 ggtcaaactg gctctgggca cgtcggcatc tcattggagg ccggcgcggc ccggaatgag
     2521 tggttccggc aatgcttgct cgcgattgga aatcaagacc agctcacgct gaccccgaaa
     2581 acggattgtc ctcgtttaca cttcaggcat ggaagaatcg cgtaccgacg agactctcat
     2641 gctggcttat cgcgatggcg atgccgcagc attcgaggtg ctctacgcgc gacatcgcgg
     2701 ccgtttgttc cgttatttgc tgcatcaatg cgccaggcgc gaacaggcgg atgaaatgtt
     2761 tcaggaggta tggatgtcgg tcattcgcgc tcgcagcggt tacgaagttt cggccaagtt
     2821 tacgacctgg ctgttccgca tcgctcacaa tcgcttgatc gacggctatc gcgcacgcgg
     2881 ccgtctggct gaattcgaag ccgacgcgac tgatttcgag ggcgaccccc cggatcatcc
     2941 ggcgccaacc agcggacagc cggagcagct ttacgagcgc gcgctggttg ccggaaacat
     3001 ggtcgccgca atcgaggcat tgccggcacc ccagcgcgaa gcctttctgt tggctgccga
     3061 gggtggcttg acggtcgagg aaatcggcaa cgcgactggc accggttatg aaaccgcgaa
     3121 aagccgtttg cgctacgcct atgcgcggct acgcagcgaa ctgagggatt tgcgatgact
     3181 cgcccgatca ttccgccgaa ggatcctatt cccgattcga ccgtgagcga aatccatgcg
     3241 ctctatcggg aaggtgccgg ggccgccgaa ccggggccga gcctggatcg cgccattctc
     3301 gatgcggcac gggctgatct ccgggccgac aatggggcga agcaaagacg gcagcttcca
     3361 tggtggaagc gctggattcc cgcaacgacc gcgattgcgg ttgcggttat cgggctgtcg
     3421 tttaccttgc gcgtctcgga gctgcaggaa agcgatctgt cagcagtgac tggcgccatg
     3481 gagcctaagc gcgatgatgc gaagattgcc gcggataagg ttccgggcaa agccacactg
     3541 tccgatcgcc cagcaaaatc gcaggctgcg agcaaggctg aggtgcctaa gccaaaccag
     3601 aatgacgagt cgaagtctgg ccagaatttc tcctctgctg ttcgcgaaat gacagccaga
     3661 ccggccccgg ccgctgagcg gcgcgctatc ccggcaagac agaggccgtc tcctgacgga
     3721 gctgcgcaag caccggcaac gatggctcaa cctgtacccg catctgaacc tcagaagaaa
     3781 tccctgcgaa gcgatgggta tggacgattg gaaaaaagtg aatcggacgc ttccggcgcc
     3841 gccggcgcgg agctcgcccg cccacccctg cggcgagaag ccgagcatat tggcgtcagt
     3901 tccagagacg gacccgcggc ggattcgatt gccccttcgc tttcaacgcg cccggacgat
     3961 gcgacaacac ctgaagcctg gctggaactc atccggaagc atcgtgcggc cggccgaaat
     4021 gccgaggccg cgcaaagcct ggcccggttt cgcatgcgtt accccgacta tgtgctgccg
     4081 gctgacctga ttcagccaaa ataggctagg cggcgcaggt gccgttttaa ccccgtttca
     4141 cgaaggcctc cgtttacagg attgcggaca tcgcaaatct ttcccccaac ggaggacatc
     4201 atgaacaaaa ccaactttgc atccctagtt cttggcgcta tcggtttcct ggctttgacc
     4261 ggctgcgcac agcccagcct tgcggcccgc ctggtcgacg tttctgtcgt caatcgcagc
     4321 accggcgaac ggctcacgcc ctaccgccac catggcaagt tctacatcgc cggtactccg
     4381 ggagacaact actccatcga actcagaagc aggcgcagtg agcgtttgct caccgttctc
     4441 tccgtggatg gcgtcaacgt actcaccggc gagacggccg cgccgttgca gtcgggctat
     4501 gtgctcgatg accgacaaag ctatgggatc agcggttggc gcaagagcat gaatgacgtc
     4561 gcccagttcg ttttcaccgc gctgcccaac agctatgcgg cgcgcacggg ccgtccggac
     4621 aatgttgggg tgatcggcgt tgccgtgttc cgtgagaagg tggttccacc cttggcgaga
     4681 tacggcacaa cggcgccatt tcccgcgaca aaggaagctc ggcacccgga accagctcgt
     4741 gatgccgatg aaggccctgt ccggcaggcg gccaagagcg aaagtgaggc gagcaccgga
     4801 gcctccaaat ccaccgcttc gggaaaactt gccgagcgac gggccaattt ggctgcagca
     4861 gactccgctt cgccggccga attcgagaaa aaggccgagc ggctcggcac cggccatggc
     4921 gagcgcgagt attccccgac gcgctacacg gggtttgagc gcgcccgcga tacgcctgat
     4981 gaaatcgtca cgatctacta cgactcccgc gacaatctga tcgcgcgcgg catcatcccg
     5041 acaccgtggc tggccgagcc cacgcccttc ccggcgaatg cccgttttgt acccgacccg
     5101 cgcagctgat cgtcaaatca ctcgcgaccg gcttcgggaa gtacgggaac ccttgggatc
     5161 gtctacaaga tagctcgggg gttccgtcca aacatctgct tcccgtcgac gacacgacct
     5221 gtgattgtgt gacgtatttc cttgtaaaca ctccattttc cttttagtct gaggccgtac
     5281 cgatccattt ctggagtctt tgatcttgga cgagtcccgt aaaaactgcg aactgtgtgc
     5341 ttcacccggc ggcgaactgg tgtggaaaag tgcgctatgt cgcgtggtct tgattgacga
     5401 tacggactat ccggggttct gccgcgtcat cctcaatcga cacgagcctg aaatgactga
     5461 tctggcagtg aatgaacggc aggcgatgat gcgcgtcgtc ttcgccgtcg aatccgcgct
     5521 acggcacctg atcaaccctg acaagatcaa tcttgccagc cttggcaacg ttacccctca
     5581 cgtccattgg cacgttatcc cgcgctggcg cgaggatcgt tgttttccca atccgatttg
     5641 gggcctggcg caaagaaccg atgtcgccct gcccgccaag atcgatctct caatactaag
     5701 aaaagcactg gccgagcaac tcggggagcc gatagtatcg cgtgaaagga gttttccatg
     5761 aatgccatgt cccccatcac ttttgcgatt catgacctgc agacaggttg cgacatcatc
     5821 tctcgcattc cattggccaa cccacaacag gccgcaatcg atctggatcg ctttctcgat
     5881 agcctgctga cctcacctcc cgatggcgag acctatttcc agttgcttga acacgcccgt
     5941 gtgccgattt cctttgtcgc ggatgaactc gccaagcgct atcagagcaa agcactccct
     6001 ctcgccgaag tcgaggaaga gacgttcgag caagtcatag cactttggcg gaaagctgct
     6061 acgtcgtacg cccgttgcgc cgaaaacgat gcgtccgatg agcaggatcc cgccagcgcg
     6121 tcacgcgttg ccacgatcct gcatcgctgc attcatcact ctggcatgat catcctcgaa
     6181 caccagcgtg ctcggcgtga gctgccatgg gggttgtggc tggaattgca tggatactac
     6241 ggaagcgccg aggaatgggg agtcgccaca ttggccgttc ctgacgccct tgatcctctg
     6301 ggacggagta ccgactgcac aaccgcatat atcgcgtttc tgatgtgcga catggccggc
     6361 agcttcagtc tctccgtgcg cgaccagact ctggtacgcc gctgggccag ccactggtcg
     6421 ccactcgtca gcctgcacaa ggcagaaccg ggcgaagccc tgccccaata cgttgtcgat
     6481 ctgatgcagg acatggcctt gcgccccgtg gccgagtgcc tgcaaaccga gcatctgcgg
     6541 cgtctggata cgactcgact gaccatgcac ataaaccagg tcaggcatca gttgagccag
     6601 aaaatccctc catcgcatct cgaactgggg gaggattgca ctgccggcca gtgcaagcgg
     6661 ctgcttgaac atcttgctcc tgcgtgggct caggctcgcg cagcgcgaaa ataccgacgc
     6721 catgctactt cgggaatcac ccagctctgt acgggttttg aggaaatgca ctatttcatc
     6781 tccgacaagg agttcgagca gccggaaaac gtgcgcgtct actccagaaa agagttcgaa
     6841 aacctgtttg ccttcaggca tcagaccgat cctcagcaag cattgcatgt gcgccaggag
     6901 caacttacct acggtgttga tagctgggag gttgtaaacc agtcagccaa tggcttccgc
     6961 ctcgtacgca gcacttccgg caagaaaatg atccacggcc agctcatcgc actccgcccg
     7021 aaggatggac agcgctttct tctggcgaag aacacatggc ttatgcagga aaagagcggg
     7081 gggctgattg cgggaataaa ggcgctgcct ggcttgccgg ttgccaccgc agcacgggta
     7141 atcgaacaag cgagtgaaag tcacgggcaa taccatcgcg cattcctgct gccggccgta
     7201 ccgtcacaag acaccgacca gtccctggtg ttacctcagg gctggttccg gaacggacgg
     7261 gtcgtcgaac tctttaccga cggcgcctgg cgggtcaagc ttcagcaggt acttgacgat
     7321 gggcctggat tcgagcgggt cagctttgtg gtctgctgaa cgcaacgctt agccgagtat
     7381 gcaacgctcg gggaagcgcg ctgaaaagcg gcttcccttg ccaagggtac tttcgatctc
     7441 cagcgtggca tgatggcgcg tcaatacatg tttgacgatg gccagtccca gcccggtgcc
     7501 acctgtctcg cgcgagcgac tccgatcgac tcgatagaaa cgctcggtca ggcgtggtag
     7561 atgctgagcg gcgataccga taccggtatc ggtcacgaca aactctccga acccgtcgtg
     7621 tacccgccag agaagatcaa tcctgccgcc cggtggagtg tatcgcacgg cattcccggc
     7681 cagattactg aaggcactac gcaactcgct ggcgtacccg gcgagcaccg aaggtgtttc
     7741 gacattcagg acaatctcgt gttttcctgc cgaaagcgcc tgcgcgtcgg ccaggatgct
     7801 gcgcagcatc ggttccagct ccacccgctc ctcattgagc cacggtcctc cggactccag
     7861 cgaagaaagg gtcagcagat cttcgatcag acgctgcatg cgcgcggtct gctcgcaaat
     7921 cagcgtcaga taattattga ttttggcacc accataagct tcatggccgc caaccagcat
     7981 ttcggaaaaa ccggcaacca cggtcaaggg cgtcttgagt tcgtgagaca cattggcaat
     8041 gaaatcgcgg cgtatggtct ccacacgctc gacctgactg acgtcacgcg agaccagaag
     8101 gttctggtct tcgccgtacg gaattatctg cagcagcaag gtcataccat cgacacgacc
     8161 gtcccgataa atcaacggtt cggcgtactg cccgctgtca agataggcaa cgaaatccgg
     8221 atggcggatc aggttggtca aaaccagtcc ccgatccgag gccgcagaca aagcaaagtg
     8281 cgtttcagca cgttcgttga tccagtcaat gcggcggtga cgatcaaatg caatcacacc
     8341 atccggcaag gcctggaaca cactcatgaa attctcaagt gtttccgcaa gcaactgctg
     8401 ctgaccgaga cgcatgcgaa ccctacggtg caactcggca aaggtggttt cccagacgcc
     8461 tcgcccttct ggcagtggac tattgagttc tccttcaagc caactcatca gtttttgaag
     8521 atgaaacaga tgccagcaca acaaaagaag caggaatata actgccgagg ccagccccca
     8581 gttgagtcca tagatgtaga agactggcgg aaacacgatg caggtgaaaa gaagcaactg
     8641 ccaaatcaaa cgtacccaga tcgcgctcat gccatattct cggattaacc ggacaatctc
     8701 attcgggcag cgccgagaaa cggtaaccac tgccgcgcac cgtctggatc aggcggtcat
     8761 gcccggtttg ttccagggat gagcgcaggc gacgaatatg aacatctacc gtccgctctt
     8821 cgacaaaaac gtgatcgccc caaacctgat caagcaactg tgtgcggcta tgaacgcgtt
     8881 cggcgtgcgt catgaagaaa tgcaacaaac gaaactcggt cggcccgaga tccagcgact
     8941 ggccatttcc ggatactcga tgcgaaacgg gatcaacgcg caagccttcg acatctacac
     9001 agtcctcggt catttgcggc gcccgacggc gcagcaccgc cttgatacgg gccagcaatt
     9061 cgcgtggtga aaaaggtttt gtcagataat cgtcagcgcc cgcctccagc cccgccacct
     9121 tgtcatgttc ctcactgcgt gcggtcagga tgatgatcgg caagtagcga gtccgctctt
     9181 cgctacgcaa acggcgggca tactgaacac cgctcattcc gggcaacatc cagtcaatca
     9241 ggacaacatc gggcagagta ctgttcacta gccggatcgc gtcttcagca gtatcggcac
     9301 gcataacgcg atgcccggca tgctgcaggc tggcggcaat caactcctgg atcgacggct
     9361 cgtcttcaac aactaggatg gtggctgaca tggtaaggtc tctctgcgta ttcacatgct
     9421 cccagtttat taaagcagga tgacaaactg atgacagtta accgattgga acacacaagc
     9481 gcacgatcca ccaacgtctt gctgcgggct tgaattgccg ttgtcgaacc ttcgatccag
     9541 ccctcccaga ggtttccaac ccgactttgc gctatcatag cgcgttcgat tacaccacaa
     9601 gaccgccatg gctggactta atcccggtac accgattccc gtcgagatca agctgcataa
     9661 caaatcccgc atcgtcgaac tcgccttcga gaacgatgaa cgttatgaac tgacttatga
     9721 attcctgcgt gtatttacgc cttcggcaga agcacgcggt cacggcgcag gccaggaaac
     9781 attgcaggtc ggcaaacgcg atgtggaaat tgtgactatc gaaccagtcg gcaactatgc
     9841 gatcaaacct gttttttccg acggccacga aagtggtctg tattcgtggg atattctata
     9901 tacgctttgt gccaaccgcg acgcgttgtg gcaagcctac cttgacaagt tgaaggcgga
     9961 aggtggaagt cgcgacccgt cggaatgccg ccccgatacc gccaaacccc caccgtcctg
    10021 cggtagcggg aagcgctgaa attttg
//
LOCUS       DAORPM010000069         9960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1562_length_9960_cov_11.016052, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000069 DAORPM010000000
VERSION     DAORPM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_9960_cov_11.016052"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="PLD53_04990"
     CDS             <1..1541
                     /locus_tag="PLD53_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC60298.1"
                     /translation="LSDAVLYHHDPAERVVAAHKLVRIIFLANRLSACTTAPGQAEID
                     LAGLCGASGVDLQLLLAKVEQELAKLAEQMGIELAETPPADAVNSESSKNANGVASNA
                     LAARVRDVILVENVLGDTLIDDGLEASLQAISQSARVLFGVHPAICFLPQSEDPARFV
                     AHPVGSRWAKASQLEFILGRSGAEVARAIDQGVLLLAPGVRPQNLLDEQLLRLIGGTG
                     LVLVPLRSQQVCHGVLVAGFESALQAEAFLEQSPCFEHFGRMAGDLLHKTRALPASDG
                     SSQANPAVDALRSHVRRVVHEIGNPLSIIQNYLSTLETKYTEGDGGGYELGIVKEEIS
                     RVAKILQTVLQEPGASQSETGAIKLNQLIQDLVALCSASGLVAGSVEIQTDLFADPPA
                     LWSDVDRLKQLLLNLLKNAMEAVSAGGAEGANIVRVSTAPWGSSKEPSHIEIRIEDSG
                     PGIPSHILDHLYQPVLSTKGQGHLGVGLAVVGELVRDLHGLINCQSNERGTRFQLLLP
                     LGKR"
     gene            1571..3820
                     /locus_tag="PLD53_04995"
     CDS             1571..3820
                     /locus_tag="PLD53_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC60299.1"
                     /translation="MQRPGGNANIFANVLATRAEHSAAFAVSFSSGAMPKSSASSGAN
                     QSEALPVQVKTHRLLVVDDDAGFRESLCFLLKRKGYPAPLACAGGRESIKLLMEMPFD
                     LVLLDLLLPDVSGLEVLGMLRQASLDVAVVVISGDSTIDSAIGALRLGADDYVRKPYV
                     SEMLLHTIENVLRRRELEKSQRQLAAELERSERKYHFLVDASPDLIFTLDEQFNFSFV
                     SKRVASQSGCGVDDLLRKPFLSIVHQEDLERTRYTLERVSQGACSTELRLIRRDESVC
                     GHFEVSLIPVDSFPANSFKGNGRRCGIYGVARDISVQKLNNDRLAFLAFHDTLTGLPN
                     RALYCDRVGLAIVQAKRNRMKVASMFVDLDRFKLANDTFGHQKGDELLKEAAGRLQNS
                     LRETDTLARIGGDEFTILLPGLRTREDAACVADKLVSDMAKPFALAGIEVFLTASVGI
                     AMYPDDGEDIETLLRHADLAMYHVKSRGKNGAGFFSSTMGEYTSGRLLFENEIRRGLE
                     SGEFELHYQCQVDSTTQKTVGLEALSRWQHPSKGLLSAGSFVPMVEEIGLMPALTYWV
                     LQTACQDMQRWRDQGLDMPRMSVNISPCVLEQDDFHERLLDILHEHSIPPECLEIEIT
                     ENVLVGDRQKVTDRLLRLSRERVRIAIDDFGTQYSSLGYLRFLPVNTLKIDQSFVREI
                     ERIDDNSPIIRAIVAIAAGLDLDVVAEGVETEVQAGFLRKLGCRKMQGFLYGQPLPVE
                     KTEALLEPL"
     gene            3898..4986
                     /locus_tag="PLD53_05000"
     CDS             3898..4986
                     /locus_tag="PLD53_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HRC60300.1"
                     /translation="MSPEALTKLFSPLGLGALTVPNRVFMAPLTRCRASAGHVPNALM
                     AEYYAQRAGAGLIIAEATMAMEGHSSFWMEPGIHSAAQVAGWRLTTDAVHAAGGRIFL
                     QIWHGGRACHPSLNGGAQPVAPSPIAITGDEVNTPEGKKPYVIPRALRDDELSGIVTG
                     FKKAAENARAAGFDGVEVHGANGYLLDEFLRDGANQRTGPYGGSVENRARLMLEVIDA
                     VSSVSGSGRVGLRISPLNSYNDMIDSDPIGLSSWLATRLNEFRLAYLHVMRSDFFQQQ
                     RGDVMTPVRANYQGVLIGNMGYTGVEAATAIARGDVDAVAFGSSFLANPDLPERIRTG
                     SALNKPDPATFYTPGAEGYTDYPSLSSS"
     gene            4996..5406
                     /locus_tag="PLD53_05005"
     CDS             4996..5406
                     /locus_tag="PLD53_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRC60301.1"
                     /translation="MSLRPNVSNAEESPLSEEFLVICLCAEWCGICRDYRAGFDGLAS
                     QFPDTRFCWVDIEDQADDLGEVDVENFPTMLIRRHQLVLFFGAILPHQSHLRMLIEAF
                     REQTPAESLAYANSNPERRGWQENQELCRLGMIK"
     gene            5659..9090
                     /locus_tag="PLD53_05010"
     CDS             5659..9090
                     /locus_tag="PLD53_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60302.1"
                     /translation="MGSTSNTAAAPLFANTAELNAAINLRLALLELPLAAGADGASIT
                     DLMAPILARQRELSRRLSDKLCPVDTRIQNFLKEYLSDVGPAPRLPGKTLVLDQPGLA
                     RGISLPANGDLYKSPLLSSYRLRNGVLHNPASDRRTTAGVFHIVEGGLPIPDDKLAVP
                     KLAFSKLLALALNPPPDSLQLPFTAGENAPAMCFVSLLLRPLVVPAVPGFTTEKRMET
                     RFFVPGALVANLDFVESIFGNAGDPNLPENDASLDPDSWTGHTGCVILAPHLTHVPKH
                     LLGLPHWDAATPLQRRDGMCYKSEAELYNGGSAFKLCARDERGVVVTLIADNYFGYCK
                     KEVKTQIGYSANLFGSAEEEHSGGALVFPSYNEGQEYTDLTAGDAYSLKEVLARDPDR
                     FAVQPAGHALDLVQPNVVLVPAKSTFSLRSQTVSWPTIDGNTSSIKLQAGKTYMGPNG
                     YRIQMESSRADHRQWNLVGTSAEVTACHKPATVSGGGKSEISKAISDAILVGNVYVGD
                     LEKDMDAVAAILERDFSERFSDQEKNGVDHRLILSSDRSIGSVIKLLTPSRRDYNEAY
                     NQWLSVIPQHIKELVFVVKRFHRPEWGDSWRSHYSVDLINGRPGNSLKLDGKTLIVNT
                     LRVGFEKDGAWRVFGLRHDFHPAVKVQTEDDITASVVAPAGAIAGESGALSQKFVQNC
                     ERRLFQRPDDAIHRGYDGQAEADIATPGTFISNFQPLDGSDARELVDDAIGFNEFTAP
                     MQELIQAAANAPDGSSPAYFVSSAHPRIVDGKPTKNPRYLQLRPDIACPDDTAIANLA
                     MRLFRRQSSAEPLITPVHVVAAGRRNNPPDEGIRPLCPYNPLHYMELPELFIEFISSM
                     TGKSPSTTGAGSEGALTKGPFNALPAIIDLNAAFVSFALTGYDGWVSSAGYVGPNVRV
                     DHDVSLLVPEVFARMSPSERDAKNLIARGALERVVDFEHGGQPVLASRLGYRMTARLA
                     STYFGRIFLHPHAVFTDEMLRPELQDMDIFADGVANIVATHQRVAESYFTDGTVSLAC
                     PPLRALLEIMAHGRTAEGHGLESAEVRSLFSRESVLASDWYAARLDAKQRRDEARLTH
                     AVASLYEFLDRTDSAEVASRLGLSARRAQAQGERVRVGSAEYRAGLVGTIGLQPL"
     gene            9226..9597
                     /locus_tag="PLD53_05015"
     CDS             9226..9597
                     /locus_tag="PLD53_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60303.1"
                     /translation="MFREGGVGLVFKSPTFWLKGSVAGFSRERRLAGRCPKIGKPVQS
                     YTRDDWVRVASSSPCVHRDADVREVTVLRIRVAVEEWETPWSNMHGTAGWLFRGQFLD
                     KPLVKGEQIDFDASWLERCEL"
BASE COUNT         1930 a         2797 c         2977 g         2256 t
ORIGIN      
        1 tcctatccga tgccgtgctc taccaccatg atccggcgga gcgggtcgta gcagcccata
       61 aactggtacg catcatcttt ctcgccaatc gcttgtccgc ttgcactacg gcaccggggc
      121 aggctgaaat cgaccttgct ggcctctgtg gtgccagcgg agtcgacttg caattgctgc
      181 ttgcgaaagt cgagcaggag ctggccaagc tcgccgaaca aatgggcatc gagttggctg
      241 aaacgccccc tgcagatgcc gtcaactcgg aaagctcaaa aaatgccaat ggcgtggctt
      301 ccaacgctct tgcagcccgt gtacgtgatg tgatactggt cgaaaacgtg ctgggagata
      361 ccctgattga cgacggcctc gaagcgagcc tgcaggccat cagccagtcg gcaagagttc
      421 tgttcggggt acaccccgcg atctgcttct tgccgcagag cgaagacccc gcgaggtttg
      481 tagcgcatcc tgtcggtagt cgctgggcca aggcttcgca gctggagttc attctcgggc
      541 gcagtggtgc ggaggtggcg cgagccatcg atcagggcgt cttgctcctc gcgccgggcg
      601 tgcggccgca aaatctgctt gatgaacaac tcctgcgcct gatcggcggt accggtctgg
      661 tgcttgttcc cctgcgtagc cagcaggtat gccatggtgt gctggtggct ggctttgaaa
      721 gcgcacttca agccgaagct ttcctggaac agagtccctg tttcgagcac ttcgggcgca
      781 tggccggtga cctgctgcac aagaccaggg cgcttcccgc cagcgatgga tcgtcgcagg
      841 ccaatcccgc tgtcgatgcg ctgcgctcac atgtgcgccg ggtcgtccac gaaatcggga
      901 acccgctttc gatcatccag aattatctat caacgttgga aacgaagtac accgagggcg
      961 atggtggtgg ctacgagctg ggaatcgtca aggaagagat cagccgcgtt gccaagattc
     1021 tgcagaccgt tttgcaggaa cccggtgcaa gtcagtcaga gaccggcgcc atcaagctca
     1081 accagctcat tcaggatctg gtagcgcttt gcagtgcctc cggtctcgtc gcgggttccg
     1141 tcgaaatcca aaccgatctg tttgccgatc ctcccgcgct gtggtctgac gtcgaccggc
     1201 tgaaacaact gctgctcaac ctcttgaaga acgcgatgga agcggtgagt gcggggggtg
     1261 cggagggcgc caatatcgtc cgcgtaagca ctgcgccgtg ggggagcagt aaggaaccgt
     1321 cgcatatcga aattcgcatt gaggattctg gaccgggaat cccgtcgcat attctcgatc
     1381 atctctacca gccggttttg agtaccaagg gacagggcca tctcggggtt ggtctggcgg
     1441 ttgtcgggga gttggtgcgc gacctgcacg gcctgatcaa ctgccaatcc aacgagcgcg
     1501 gaacccgttt ccagcttctt ctgcctctgg gtaaacggtg atccatccca gttctgccgc
     1561 ctctcgtgcg atgcagcgtc cgggcggtaa cgccaatatc ttcgccaacg tactcgccac
     1621 acgtgctgag cattcggctg ctttcgcagt ctccttttct tctggcgcca tgccgaagtc
     1681 gtctgcgtcg tctggagcga atcagtcgga ggcattgccc gttcaggtga agacgcatcg
     1741 tctgctcgtg gtggacgacg acgccggttt ccgtgaaagc ctgtgctttc tgctgaagcg
     1801 caagggctat ccagcgccct tagcctgcgc aggcgggcgc gaatcgatca agctgctgat
     1861 ggaaatgccc ttcgatctcg tcctgctcga tctgctgttg ccggacgtga gcgggctgga
     1921 agtgctggga atgctgcgcc aggcaagtct ggacgttgcc gtcgtggtaa tcagcggcga
     1981 ttcgacgatt gattcggcca tcggagcctt gcgcctcggc gccgacgatt acgtgcgcaa
     2041 accgtacgtc tcggagatgc ttcttcacac catcgagaac gtgctgcggc gacgcgaact
     2101 tgaaaaatcg cagcgccagt tggcggccga gctggagcgc tcggagcgaa agtaccattt
     2161 tctggtggac gcctctccgg acctgatctt tacgctggat gagcaattca acttttcttt
     2221 tgtcagcaag cgcgtcgcca gtcagagtgg ttgcggcgtt gacgacctcc tgcggaagcc
     2281 gtttctgtcg attgttcacc aggaggatct ggaaagaacg cgttatacgc ttgagcgcgt
     2341 cagtcaaggc gcgtgttcga cggaactgcg tcttatccgt cgggatgaat ccgtgtgcgg
     2401 gcatttcgag gtcagcctga ttcctgtgga ttcctttccg gcgaattcct tcaaaggcaa
     2461 cgggcggcgc tgcgggatct acggtgtggc gcgcgatatc agcgtacaga aactgaacaa
     2521 cgatcggttg gcttttctgg cctttcacga taccctgacc ggcctgccga acagggcgct
     2581 gtattgtgat cgcgtcggac tggcaatcgt ccaggcgaag cgcaatcgca tgaaagtggc
     2641 ctccatgttt gtcgacctcg atcgcttcaa actggccaat gacacctttg gccatcagaa
     2701 gggggacgag ttgctgaaag aggcggcggg gcgcttgcag aacagtctgc gagaaacgga
     2761 taccctggct cgcatcggtg gcgacgagtt cacgatattg ttgccaggct tgcgtacccg
     2821 tgaagatgcg gcttgtgttg ccgacaagct ggtctccgat atggccaagc ctttcgcact
     2881 tgccggcatc gaggtttttc tgaccgccag cgtcggcatc gcaatgtatc ctgacgatgg
     2941 ggaagatatt gaaaccttgc tgcgtcatgc cgatcttgcg atgtatcacg tcaaatcgcg
     3001 tggcaagaat ggtgccggat tcttctccag caccatgggt gagtacacca gcggtcgcct
     3061 gttgttcgaa aacgaaatcc ggcgcggact cgaaagcgga gaattcgaat tgcattatca
     3121 gtgtcaggtc gattctacta cgcagaagac cgttgggcta gaggcgctga gtcgctggca
     3181 gcatccgtca aagggcctgt tatcggccgg tagcttcgtc cccatggtcg aagagatcgg
     3241 tctcatgccg gcgctcacgt actgggttct gcagacggca tgccaggata tgcagcgctg
     3301 gcgggatcag ggccttgaca tgccgcgcat gtctgtgaat atctcgcctt gtgtgcttga
     3361 gcaggacgat ttccatgagc gcttgctcga cattctgcac gaacactcca ttccacctga
     3421 gtgtctcgaa atcgaaatca ctgaaaatgt ccttgtcggc gatcgtcaga aagtgaccga
     3481 tcgcctgttg cggctttcac gggagcgcgt tcgcattgcc atcgacgatt tcggaaccca
     3541 atattcatcg ctcggctacc ttcgatttct gccggtcaac actctgaaaa tcgaccagtc
     3601 tttcgtgcgc gagatagaac gtatcgatga caattcaccc atcattcgcg ccatcgtcgc
     3661 gattgcagcg gggctcgacc tcgatgttgt cgccgaaggc gtggaaactg aagtgcaagc
     3721 tggctttttg cgcaagctcg ggtgtcgcaa gatgcaaggt ttcctgtatg gccaacccct
     3781 gccggtcgag aaaaccgaag cgctgttgga gccactttag gtaagagcag cgtcgcgcgc
     3841 caggccttaa ttcgtccgcc ttcgtttccc gctgactaat ccactatgaa agatttcatg
     3901 agcccagaag ctttgaccaa gctcttcagt ccgcttgggc ttggagcgct aacggttccg
     3961 aaccgcgtct tcatggctcc gttgacgcgc tgccgggcaa gtgccgggca cgttcccaac
     4021 gccttgatgg ccgaatacta cgcacagcgc gccggcgccg ggctgattat tgccgaagcc
     4081 acgatggcga tggagggtca ctcgtcattc tggatggagc cgggaatcca ttccgctgcc
     4141 caggttgcgg gctggcggtt gaccaccgac gctgtccatg cggccggtgg ccgcatattc
     4201 ctgcagatct ggcatggcgg tcgcgcatgc catccttcgc tcaatggcgg cgctcagccc
     4261 gtcgcgccca gccccattgc cattaccggg gatgaagtga atacgccgga ggggaaaaag
     4321 ccctacgtca ttccccgcgc gctgcgcgat gatgagttgt cggggattgt taccggattc
     4381 aaaaaagcgg ctgagaatgc cagggccgcg ggtttcgacg gcgttgaagt ccatggtgca
     4441 aatggctatt tgctggatga attcttgcgt gatggtgcaa atcagcgcac aggcccttac
     4501 ggagggagcg tcgaaaaccg agcccggttg atgctcgaag tgatcgatgc ggtcagcagt
     4561 gtatcgggca gcggccgagt cggcttgcgt atttcgccgc taaacagcta caacgacatg
     4621 atcgacagcg acccgatcgg cctttcctct tggctcgcta cccggcttaa cgaattccgg
     4681 ctggcgtatc tccatgtcat gcgcagtgat ttctttcagc aacaacgcgg tgatgtaatg
     4741 acgcctgtca gagcgaacta tcaaggcgtt ctgatcggca atatgggcta tacgggcgtc
     4801 gaggccgcca ctgcaattgc tcggggcgac gtcgatgcag tagcgtttgg ctccagtttc
     4861 ctcgccaatc ccgatctgcc ggagcggatc aggacaggct cagcactgaa caagcccgat
     4921 ccagccacgt tctatactcc cggcgcagag ggctatacag actatccatc gcttagttct
     4981 tcgtaacggt caatcgtgtc gctgcgtcca aatgttagta atgccgaaga gagtccgctg
     5041 tcggaggagt ttctggtgat ctgcctgtgc gccgaatggt gcgggatctg tcgtgattac
     5101 agggcgggtt tcgatgggct ggcgtcgcag ttcccggata cacgattctg ctgggtcgat
     5161 attgaagacc aagccgacga tctcggggag gtggacgtcg agaattttcc gacgatgctg
     5221 attcggcggc accagttggt gctttttttc ggggcgatac tgccgcacca gagccatttg
     5281 agaatgctga ttgaagcatt ccgggaacaa acacccgcgg aaagcctcgc ctatgcaaac
     5341 tccaatccgg agcggcgagg ctggcaggaa aatcaggaac tttgtcgcct gggcatgatc
     5401 aaatgacggc acggcgggct tgctggtatc tgtgggatca tgttttactg agcgctctga
     5461 tcgatccagc atcccggcct atgccgacta ggcacgcgaa tactaagtcg aaatcctgct
     5521 cccttgaagg atgtctgcag actaggctga tttccggcat attgcgttcg taaccgcgtt
     5581 ctttatcaca gccttttggg gcaatcattg cctatcgacc gcttaacact gtgtcctcac
     5641 tctacgaaag aatacgatat gggttccacc tcgaataccg ctgcagcccc gctatttgcc
     5701 aatacggcag agttgaacgc cgcgatcaat cttcgccttg cccttctcga actgcctctc
     5761 gctgccggtg ctgatggtgc gtccattacg gatctgatgg caccaattct ggcgcgccaa
     5821 cgggaattga gccgtcgact ctccgataaa ctttgccctg tcgatacgcg gattcagaac
     5881 tttctgaagg agtatctttc ggatgtgggt ccggcgcccc gtttgccggg taaaaccctg
     5941 gtgctggacc agcccggtct ggcgcgcggc atttcgttgc cggccaatgg tgatctttac
     6001 aagtcgccgc tgctcagcag ctatcgtctg cgcaatggcg ttctacataa tcccgccagt
     6061 gatcgccgca ctactgccgg cgtttttcac atcgtcgagg gcggtttgcc gattccagac
     6121 gataagttgg cggtgccgaa gctggcgttt tccaagctgc ttgccttggc gctgaatcct
     6181 ccgccggatt cgcttcagtt gccattcacg gctggggaga atgccccggc catgtgtttt
     6241 gtatccctgc ttttgcgtcc gctcgtggtg cctgccgttc ccggttttac gacagagaag
     6301 cggatggaaa cccgcttttt cgttcccggg gctctggtgg caaatcttga ttttgtcgaa
     6361 agcattttcg gcaatgccgg cgatcccaat ctgccggaaa atgatgcctc gctggacccc
     6421 gatagctgga ccgggcacac gggctgcgta attctcgcgc cgcatcttac gcatgtaccc
     6481 aaacacctgc tcggacttcc tcactgggat gcggccacac cgctgcaacg gcgtgacggc
     6541 atgtgctaca agagcgaagc cgaactgtat aacggcggca gcgcattcaa gctgtgcgcg
     6601 cgcgacgagc gtggcgtggt cgtgacgctc atcgctgaca attatttcgg ctactgcaag
     6661 aaagaggtga aaacgcagat cggctattcg gcgaacctgt tcggttccgc cgaagaagag
     6721 cattccggcg gggcgctcgt gttcccgagt tacaacgagg ggcaggagta cactgacctg
     6781 accgcgggtg acgcctactc gctgaaagag gtgttggcgc gcgatcccga tcgctttgcc
     6841 gtgcagccgg ccgggcatgc acttgatctc gttcagccga atgtcgtgct ggtgcccgcg
     6901 aaatccacct tcagtttgcg ctcgcagacc gtctcctggc caaccatcga tggcaacacg
     6961 tcatcgatca aattgcaggc gggtaagacc tatatgggtc ccaacggcta ccggatacaa
     7021 atggagtcat cgcgtgccga tcatcgtcag tggaatctcg tcggaacatc ggcggaagtg
     7081 accgcctgcc acaagcccgc gactgtttcc ggggggggga agtcggagat atccaaggcg
     7141 atttcggacg cgatacttgt cggcaatgtt tacgtcggcg acctcgaaaa ggatatggat
     7201 gcggttgcag ccattctgga gcgcgatttt tccgagcgct tcagcgatca ggaaaagaac
     7261 ggcgttgatc accgtctcat actcagcagt gaccgctcga tcggtagcgt catcaagctg
     7321 ctcacaccat cgaggcgaga ctataacgag gcgtacaacc agtggttgag cgttattccg
     7381 caacatatca aggaacttgt cttcgtcgtc aagcgctttc accgcccgga gtggggcgat
     7441 agttggcgga gtcattactc ggtcgatctg atcaacggac gtccggggaa ttcgctgaag
     7501 cttgacggaa agacgctgat tgtgaatacc ctgcgcgtcg gcttcgagaa ggatggagct
     7561 tggcgtgtct tcggcttgcg ccatgacttc caccctgccg tcaaggtaca gaccgaagac
     7621 gacattaccg ccagcgtagt cgcacccgcc ggagcgattg ccggagaatc gggggcgtta
     7681 tcgcagaagt ttgtacaaaa ctgtgagcgt cgcctgtttc aaaggccgga cgacgcgatc
     7741 catcgaggtt atgacggtca ggccgaagcg gatatagcga caccgggaac cttcatatcc
     7801 aatttccagc cgcttgacgg ttcggatgcg cgtgaactgg ttgatgacgc tatcggtttc
     7861 aatgagttta ccgcaccgat gcaggagctg attcaagctg ctgcgaacgc gcccgacgga
     7921 tcgtctcccg cctattttgt gtcctcggct catccgcgca tcgtcgacgg gaagccgacg
     7981 aagaacccgc gctatctgca gctgcgcccg gatatcgcgt gcccggatga cactgcaatt
     8041 gccaacttgg caatgcgttt gttccgcagg cagtcaagtg ccgagccgct cattactccg
     8101 gtgcatgtgg ttgccgccgg tcgccggaac aaccctccgg atgaaggcat ccgtccgcta
     8161 tgcccctaca atccgttgca ctatatggag ctgcctgagc tcttcatcga gttcatcagt
     8221 tcaatgaccg gcaagtcacc ctcgacaacc ggcgcgggct cggagggtgc gttgaccaag
     8281 gggccgttca atgcattgcc ggccatcatc gacctgaatg ccgctttcgt gtcttttgcg
     8341 ctcacgggtt acgacggatg ggtgtcctct gccggttacg tcgggccaaa tgtgcgcgtc
     8401 gatcatgacg tcagtttgct ggtgcccgag gtatttgcac gcatgagtcc aagtgaacgc
     8461 gacgcgaaga atttgattgc ccggggcgcc cttgaacgtg ttgtggattt cgagcacggt
     8521 ggtcagcctg ttttggccag tcggctcggt tatcggatga cggcgcgatt ggcgagtacc
     8581 tactttggcc ggatatttct gcatccgcat gccgtgttca ccgatgaaat gctgcgcccc
     8641 gagcttcaag atatggacat ctttgctgac ggagtggcga acatcgtcgc aacgcaccag
     8701 cgtgttgccg aaagctattt taccgatggc actgtttctc tggcctgtcc gccattgagg
     8761 gccttgctgg aaatcatggc gcacggacgc actgcagagg gtcatgggct ggagtcagcg
     8821 gaagtgcgat cgctgttttc ccgcgaaagc gtcctcgcca gcgattggta tgcggcacgc
     8881 ctggacgcca agcagcggcg tgacgaggcg cggttgaccc atgccgtggc ctcgctatac
     8941 gaattccttg atcgcaccga tagtgccgaa gtggcctccc gccttgggct ctcggcgcga
     9001 cgtgctcagg cacagggtga gcgggttcgc gtcggctcgg cggagtatcg tgctggcctc
     9061 gttgggacta tcggcctgca gcccttataa cgcgtgttgg ctatcgggcg gttcggccgg
     9121 tgcctgttct ttctgtgcgg acaggttctg gccacttccg cgtcagccga cgaggctcca
     9181 gcagcttcgt gggcttcggc cgatcctcgc ctgggcgagt gcgtcatgtt tcgcgaaggc
     9241 ggtgtaggtc ttgttttcaa gtcgccgaca ttctggttga aaggctcggt tgccggtttt
     9301 tccagagagc gccggctggc cggtcgttgc ccgaaaatcg gcaaacctgt gcagtcctat
     9361 acgcgcgacg actgggtacg agttgcttcg tcctcacctt gtgtccatcg cgatgccgat
     9421 gtgcgtgaag tgactgtgtt gcgcatccga gtagccgttg aggagtggga gacgccttgg
     9481 tcaaacatgc atggaacggc tggctggctg tttcgtggcc agttccttga caagccgctg
     9541 gtaaagggcg aacagatcga ttttgacgcg tcctggctgg agcgatgcga gctatagccc
     9601 tgaagaagcg cttccgggct cccgatttct gcctccagtc agtcgccttg ctgactgaca
     9661 acggcttcag tcaagctgct tcgaaccgcc gccttgtaat ccgtgggcag tcgacggttt
     9721 gcgccggagg cgaatgacgc cggtattgat gatgtggcgc gattgctttg cgtagcggca
     9781 ccagaaaagg cacttgtcgg catgatttct gctatagtat cgcccgaatc aatataaacc
     9841 gtttgacaat ctttggttgg cgcgtcagaa tttccggttc cgagttggag ggatacccaa
     9901 gcggtcaacg ggatcagact gtaaatctga cggctctgcc ttcgaaggtt cgaatccttc
//
LOCUS       DAORPM010000070         9942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1566_length_9942_cov_9.221604, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000070 DAORPM010000000
VERSION     DAORPM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1566_length_9942_cov_9.221604"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /gene="dnaX"
                     /locus_tag="PLD53_05020"
     CDS             complement(<1..1385)
                     /gene="dnaX"
                     /locus_tag="PLD53_05020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRC60304.1"
                     /translation="MTYQVLARKWRPKTFESLIGQEHVVRALTHALTEQRLHHAYLFT
                     GTRGVGKTTLARILAKSFNCEVGITATPCGVCSACTEIDSGRFIDLLEVDAATNTKVD
                     EMRQLLENAVYAPTRGRFKVYVIDEVHMLSNSAFNAMLKTLEEPPEHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMTPALIAGHLRHILEVESISAEPGALNLLARSAAGSMRDAL
                     SLLDQAIAHGAGKVEEAQVREMLGTVDLDYLFSILDALLAGDAAAMLAVADDMAMRSL
                     SFDAALQEMSNLFTRLQVAQLAPQAVADDLPERARILELAAQLDPEFVQLAYQIAVHG
                     RKELPLAPDDQAGFVMTLLRLHAFRPTSGGEVATPGAAAVVPQAHGTAAPATIKPIAK
                     DVPSATVRGGRANEVDPAPPPASVAPEIPVAKVTEETAAMASVDADWHQILVAIKLGG
                     MARELGQHCELR"
     gene            complement(1477..2604)
                     /locus_tag="PLD53_05025"
     CDS             complement(1477..2604)
                     /locus_tag="PLD53_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRC60305.1"
                     /translation="MRRARYRSGPLVWLAILFTTCLLLFLFQKILWLVVPFLLALIGY
                     YVFFPLQQRLVLGGMSRATSAALVSGGALAVAVGIVLLAFPWISAHLVSWQDSAARYI
                     EGGLRFIAETLAWAERNYIFAAKAQLSDQLTKNISDLGDKFAGSYLTTAMLTAAAWLP
                     SLFLAPFLAFFFLRDGWRFKMFLSRAVPNAFFERTLYLLDQVDHTARLYFQGLIKLTL
                     IDALCLAFGLSLIGVSAPFVLGFVTAVLAWVPYIGSILGCLLVVLVAATDFPSNPAMA
                     YAAIGLFVWVRLLDDFFFMPITIGRSLNLHPLLTVVMIFVGGAVAGVAGLMLVLPLLG
                     IVMVLGETIGEIVTDPRLRARHVFARELRHRQVTRDLQTQR"
     gene            complement(2601..3086)
                     /locus_tag="PLD53_05030"
     CDS             complement(2601..3086)
                     /locus_tag="PLD53_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRC60306.1"
                     /translation="MLNLFAKNKPAQRLDDLKRLLLFVDLNTREMKIVDGFMHERSYL
                     KDEVIFDEGEEGQAIYFILAGKVLICHQGESESPIATLERGNFFGELALLDDAPRSAQ
                     ARAGGNCTLAVFFRGDFLGLMNSHAVIASKIALQLARHLGMRLRSIVHSEQEIGEQVH
                     P"
     gene            3318..3512
                     /locus_tag="PLD53_05035"
     CDS             3318..3512
                     /locus_tag="PLD53_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60307.1"
                     /translation="MDALVKLGRIAGVAGAVLCALGAGFRLGGAYWIAGFQSGTLLLA
                     GSAAMIFGCFCLLVVLTQRP"
     gene            complement(3547..5070)
                     /locus_tag="PLD53_05040"
     CDS             complement(3547..5070)
                     /locus_tag="PLD53_05040"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRC60308.1"
                     /translation="MPMEIELKLRLSPTQARRLQAQPALSGHTPQKYRLRNTYFDTPD
                     FELRSRGIALRLRQKGWAVWLMTVKSGASGAGGLAQRSEWEAPTKPGVFDFSIVTDDA
                     LRVFLESQHSRLQPVFSTDFTRTAWKLSLAGSVVELAFDRGRINAPGIGGTSASVSKP
                     LCELEIELIEGESPDALFDLAIELATELHLHPEIFSKAERGYALADGTLANPVKAFAS
                     NVNKEMSPVDAFRSVALSCLAHLQRNEAGAVAGENPEYVHQARVAIRRLRSAFRLFAP
                     VLAVDFVNVYSPRWRELAGGLGSARDWDVFLAETLAPLEEAFPEDADLAVLRARGETT
                     QAKAQAAAGVTLKQKAYSQLLLAFTAALFRVGPPTIEAQGKASGMGLRKFATRRLQKR
                     AEMIELLVSEHAKMNAESRHELRIAFKKLRYALEFFAPVLPQKPLAAYQTSLAKIQNL
                     LGTLNDQVTASSLIKEMHPKGERDLVTRGWIAGRTQLLIKALSSELRKFVAGKKPWS"
     gene            5392..6435
                     /gene="pstS"
                     /locus_tag="PLD53_05045"
     CDS             5392..6435
                     /gene="pstS"
                     /locus_tag="PLD53_05045"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HRC60309.1"
                     /translation="MSKFIKVLAVSMVAASASMAAHAAEVTGAGASFPAPIYAKWADG
                     YQKATGNRINYQSIGSGGGIKQITAKTVDFGASDMPLKPEVLDKDGLMQFPTVIGGVV
                     PVLNVAGIKPGELKLTGPVLADIYLGKINKWNDKAIAELNPGLALPADDIGVVRRADG
                     SGTTFIFSNYLSKVSAEWKQKVGEGTAVQWPVGLGGKGNEGVSAFVQRLPGSIGYVEY
                     AYAKQNNMTYAILKNSAGSFVAPDDATFKAAAAHADWSKSAFYEILTDEPGKDSWPIT
                     GATFILMHKVQDKPAQAAEAIKFFDWSYKNGDKMATDLNYVPLPDSLIKLIHSSWSQL
                     KDASGKPVVAATK"
     gene            6508..7485
                     /gene="pstC"
                     /locus_tag="PLD53_05050"
     CDS             6508..7485
                     /gene="pstC"
                     /locus_tag="PLD53_05050"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HRC60310.1"
                     /translation="MSAVLDSKATGASARNHKLGDWLFFNLTRGFAAMTLVLLAGIVV
                     SLVISSRLSIEKFGLGFLWSSDWNPPMEEFGALVPIYGTLSTSLIALIIAVPVSFGIA
                     LFLSELSPVWLRRPLGIAIELLAAVPSIVYGMWGLMVFAPIFAKYVQPVLSGTLGKVP
                     LVGALFAGAPLGIGILSAGIILAIMIIPFIASVMRDVFEVTPAMLKESAYGVGCTTWE
                     VVWNIVLPYTRVGVIGGIMLGLGRALGETMAVTFIIGNSNFLSSISLYAPGNSITSAL
                     ANEFGEATPGIYTSSLIELGLILFFITMVVLVFSKLLLVSLSKQEGTKT"
     gene            7493..8335
                     /gene="pstA"
                     /locus_tag="PLD53_05055"
     CDS             7493..8335
                     /gene="pstA"
                     /locus_tag="PLD53_05055"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRC60311.1"
                     /translation="MDLLKRREITNKVALTLSMAAMVFGLFWLAWILFTTLQLGVAGL
                     SLDLFTKMTPPPGAAEGGLANAIVGSLYMVALATLVGAPIGILAGIYLAEYGQHTWLG
                     EATRFINSILLSAPSIVIGLFVYAIYVARVGNFSALSGVIALALIVTPVVLSTTENML
                     LLVPNTLREAAFALGAPKRVVIMNVTLRASKAGVITGILLAVARIAGETAPLLFTALS
                     NQFWSLDLNEPMANLPVTIFKFAMSPFQDWQELAWAGVLLITLGVLGLNIASRIFFRA
                     KHKL"
     gene            8349..9152
                     /gene="pstB"
                     /locus_tag="PLD53_05060"
     CDS             8349..9152
                     /gene="pstB"
                     /locus_tag="PLD53_05060"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRC60312.1"
                     /translation="MNDTTHLISLSEKATALEAQMVFKNLDFYYGKFHALKAIDLEIY
                     KRHVTAFIGPSGCGKSTLLRTMNRMYDLYPEQRASGELLLDGMNILDRKMDVNALRAK
                     VGMVFQKPTPFPMSIYENIAFGVRLNERLSKGQMDERVEWALRKAAIWEETKDKLAQS
                     GMSLSGGQQQRLCIARGIAVKPKVLLLDEPTSALDPISTMYIEELISELKTEFTIAIV
                     THNMQQAARISDYTAYMYLGEMVEFGVTDQVFIKPKKKETEDYITGRFG"
     gene            complement(9169..>9942)
                     /gene="folP"
                     /locus_tag="PLD53_05065"
     CDS             complement(9169..>9942)
                     /gene="folP"
                     /locus_tag="PLD53_05065"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRC60313.1"
                     /translation="VMGIVNLTPDSFSGDGLATDAKRAIEYAFHQIDSGADLLDLGAE
                     SSRPGAIPAPLDEELGRLLPVLNALADCGVPISVDTYKPEVMRAAIARGASMINDIYA
                     LRMPGALEVVAESDCAVCLMHMQGEPLTMQQSPHYDDVVGEVRNFLGQRVARAVAGGV
                     AYDRLVLDPGFGFGKTLEQNLELLRCFDALATDGLPMLAGISRKSMLGAITGKSVEQR
                     LSGSLAAAVLAAQRGARILRVHDVAETKDALAVWRAASR"
BASE COUNT         2193 a         2944 c         2751 g         2054 t
ORIGIN      
        1 cgtaattcgc aatgctgccc aagttcacgt gccataccac caagtttgat tgcgaccaga
       61 atctggtgcc agtcagcatc tacagaagcc attgccgcag tttcttccgt taccttggcg
      121 acaggtattt ccggagcaac ggatgcgggc ggcggagcag gatcaacttc gttcgctctc
      181 ccgcccctca cagtggcaga cggtacatcc ttggctatgg gcttgatcgt tgcaggcgct
      241 gctgttccgt gcgcctgcgg cacgaccgca gcagcccccg gtgttgccac ttcaccgccc
      301 gaggtagggc ggaaagcgtg caggcgcaag agtgtcatga cgaaaccggc ctggtcgtcc
      361 ggcgccagag gcagctcctt gcgtccatgc acggcaatct gataggccag ttgcacgaat
      421 tccgggtcaa gttgcgccgc caactccaga atgcgcgccc gctcgggcag gtcatcggcg
      481 acagcctgtg gcgctagctg cgcaacctgc agacgcgtaa acaggttgga catttcctgc
      541 aacgccgcat cgaacgacag gctgcgcatg gccatatcgt cagccactgc cagcatggcg
      601 gccgcgtctc cggccagcaa ggcatcgagt atcgaaaaga gataatccag atcgacggtg
      661 ccaagcattt cgcgcacctg cgcttcctcg actttgccgg ctccgtgagc gattgcttga
      721 tcgagcaaag aaagggcgtc gcgcatgctg cctgcggccg agcgcgcgag caggttcagc
      781 gcgccgggct cggcgctgat cgattcgact tcgagaatgt gcctcagatg gccggcaatc
      841 agtgctggtg tcatttgctt gaggttgaac tgcaggcaac gcgacagcac cgtaaccgga
      901 attttctgtg gatcggttgt cgccagaatg aatttcacat gctcaggagg ctcttccagc
      961 gtcttcaaca tcgcgttgaa ggccgaattc gagagcatgt ggacttcgtc gatgacgtag
     1021 actttgaacc gcccgcgcgt cggcgcatac acggcgtttt cgagtaactg ccgcatttca
     1081 tcgaccttgg tgttggtcgc cgcgtcaact tcgagcagat cgatgaagcg gcccgaatcg
     1141 atttctgtgc acgccgaaca gacgccacag ggcgtggctg tgatgccgac ttcacaattg
     1201 aacgatttgg caagaatcct cgccagcgtg gtcttcccga cgccccgcgt gccggtgaat
     1261 agataggcgt gatgcaggcg ctgctcggtc agggcgtggg tcaatgcccg gacgacgtgt
     1321 tcctgcccga tcaggctctc gaaggttttc ggtcgccact ttcgcgccag tacttggtaa
     1381 gtcatgtgtg gattttaccc cgtattcttg atcggcactg cggggcgcat gctgcgcaaa
     1441 ggaggttcac ggcgattcgt ccgaaatccg cctgccctat ctctgggtct gaagatcgcg
     1501 tgtcacctgt cgatgtcgca attccctggc gaaaacatgt cgtgcacgca aacgcggatc
     1561 ggtaacgatc tcgccgatcg tttctccgag caccatgacg atacccagca ggggcaacac
     1621 cagcatcagg ccggcaacac cggcgacggc gccgccgaca aaaatcatca ctaccgttag
     1681 cagtggatgc aggttcaaac tgcgcccgat ggtaatcggc atgaagaaaa agtcatccag
     1741 caagcgcacc cagacgaaca aaccaatggc ggcatatgcc atcgccggat tggatggaaa
     1801 atcggtcgcg gcaaccagaa cgaccagcag gcaacccaga attgagccga tatacggcac
     1861 ccatgcgagg acagctgtta cgaagcccaa cacgaagggt gccgaaacac cgatcagcga
     1921 gagcccgaaa gccaggcaca atgcgtcgat caaggtcagc ttgatcaggc cctggaaata
     1981 gagcctggcg gtgtggtcaa cctgatccag aagatacagc gtgcgttcaa agaacgcatt
     2041 cggcacggca cgactgagga acatcttgaa gcgccatccg tcccgcagga agaaaaacgc
     2101 gagaaacggc gccagaaaca gcgacggcaa ccaggccgcg gcggtcagca tcgcggtcgt
     2161 cagataactc ccggcaaact tatcgcccag atcgctgata ttctttgtta actgatcact
     2221 tagttgggcc ttggcggcaa aaatatagtt tcgctcagcc caggccagtg tttcggcaat
     2281 aaaacgcaaa cctccctcaa tgtagcgcgc tgccgaatcc tgccacgaaa cgagatgcgc
     2341 gctgatccat gggaaagcca gcagcacgat gccaacggcc acagccaacg cgcctccact
     2401 gaccagcgca gccgacgtgg cacgcgacat tccgccaagg accagccgtt gctgcaacgg
     2461 gaaaaacacg taatacccaa tcaaagcgag aagaaacggt acgaccaacc agagaatctt
     2521 ctgaaacaga aagagcagca gacacgtggt gaaaagaatc gccaaccaca ccagtggccc
     2581 cgagcgatag cgcgcccgtc tcatgggtgc acttgctccc caatctcttg ctcgctatgc
     2641 actatgctgc gcaaacgcat tccgaggtgg cgagccagct gcaaggcaat tttcgaggcg
     2701 attaccgcat gtgaattcat caagcccaga aaatcgccac gaaagaacac tgcgagggtg
     2761 caattgcccc ccgcacgcgc ctgcgctgag cgcggcgcat cgtcaagcaa cgccagctcg
     2821 ccgaaaaaat tgccgcgttc gagtgtggca atcggacttt cgctctctcc ttgatggcaa
     2881 atcaatacct tgccggcaag gatgaaataa atggcttgcc cctcttcacc ctcgtcgaag
     2941 atcacttcgt ccttcaggta gctacgctcg tgcatgaacc catcgacgat cttcatttct
     3001 cgggtattca ggtcaacgaa cagcaatagt ctcttcaggt catccaaacg ctgtgcaggc
     3061 ttgtttttgg caaataggtt gagcatcgca gtcggtagta attgataggg aagaacgcga
     3121 ggttatacgt aacccaaggg attctaaagc aaagcgcatg acgcgaccat gagcgaaata
     3181 ctccgcatgc acgggaagca cccggcacct gatccgtact caggcgcttc tctactgact
     3241 atagctggat cgaagtaatt tcgggtatcc tcgggtgacg tgaatgcttc gctgacccgg
     3301 aatcaacagg aggagttatg gatgctttgg tcaagctggg gaggatcgcc ggagtggccg
     3361 gcgcggtgct gtgcgctctt ggcgccggat ttcgcttggg tggcgcctac tggatcgccg
     3421 gttttcagtc cggaacattg ctactggcgg gttctgccgc gatgatcttc gggtgttttt
     3481 gcctgctcgt ggttctgact cagcggcctt gaatcttcgc agggttgatg tccgtcggct
     3541 tgtctctcag ctccagggtt tcttgccggc cacgaactta cgcagttcgg agctcagcgc
     3601 ttttatcaga agctgcgttc gacccgcaat ccagccgcgg gtcaccaaat ctcgttcgcc
     3661 tttcggatgc atctccttga tcagacttga agcggtaacc tgatcgttca gcgttccgag
     3721 caagttctgg atcttcgcta gtgaagtctg ataggccgcc agaggcttct gcggtaaaac
     3781 cggggcaaaa aactcaagtg catagcgcag cttcttgaac gcaattcgca gctcatgtcg
     3841 gctctcggca ttcatcttcg cgtgttcgct caccagcagc tcgatcatct ctgctctttt
     3901 ttgcagccga cgcgtggcaa acttgcgcag tcccataccc gacgctttgc cttgtgcttc
     3961 gatcgtcggc ggcccgactc tgaacagtgc cgcagtaaag gccagcaaga gctggctgta
     4021 cgccttctgc ttcaaggtca cgccagccgc tgcctgcgcc ttggcctgag tcgtctcacc
     4081 ccgggctctt aacacggcaa ggtcagcatc ctcggggaaa gcctcttcca gcggagctag
     4141 cgtttcggcg agaaaaacgt cccagtcacg tgcactaccg agcccccccg caagctcgcg
     4201 ccagcgtggt gaatagacat tgacaaagtc gacagcaagt acaggggcga atagtctgaa
     4261 cgcagagcgc agacgccgaa tggctactct ggcctgatgg acgtactccg ggttttcccc
     4321 ggcaactgcc cccgcttcat tgcgttgcag gtgggccagg cacgatagcg caactgaacg
     4381 aaacgcatcg accggagaca tctccttgtt gacgtttgaa gcgaacgcct tgacaggatt
     4441 tgccagggtc ccatcggcca gcgcatagcc acgctctgcc ttgctgaaaa tctcgggatg
     4501 aagatgcagt tcggtagcca gctcgatcgc aagatcaaag agcgcgtcag gcgattcgcc
     4561 ctcaatcaac tcgatttcca gctcacaaag tggtttgctg accgacgcag acgtgccgcc
     4621 aatccctggc gcgttgatcc tgccccggtc aaacgccagt tcgacgaccg acccggccaa
     4681 gctcaacttc catgcagtac gcgtaaagtc cgtcgaaaat acaggctgaa gccgggaatg
     4741 ttgagattcc agaaacacac gcaatgcatc atcggtaacg atggaaaaat cgaacacgcc
     4801 gggtttggtt ggggcttccc actcactacg ctgggccaaa ccgcccgcac cggaagcacc
     4861 gctttttact gtcatcaacc agacggccca gcctttttgc cgcaaacgca gggcaatgcc
     4921 gcgactgcgc agttcaaaat ccggggtgtc aaaataagtg ttacgcagac gatacttctg
     4981 cggtgtatgt ccggacaacg ctggctgcgc ctgcaatcgt cgcgcttgag taggagacaa
     5041 acgcaatttg agctcgattt ccataggcat gggggcctcc cttcggccgg tcagtctatc
     5101 agggatgaaa gtcacttgtc ttgctcaaca tcattacggt attgttacag tcggagtcag
     5161 gaaacatacg catcgaacct caatatgact tcccttgaca ccccgcactt cttccctctt
     5221 ggaacccgca atcgacgcaa cggcacaggc ttgcttgatc tattcggagc gacattcctg
     5281 aaatcggctt cggatttcca ggctgatttc gaaactgtaa catcgctgaa atatttgaca
     5341 gttagcatag cctcgcttgc acgttccatc catccagaag aggagtaata catgtcgaaa
     5401 ttcatcaagg ttctagcggt atcgatggtg gcagcttcag catcaatggc cgcgcacgcg
     5461 gctgaagtta ccggcgccgg tgcatccttc cccgctccga tctacgccaa gtgggccgat
     5521 ggctatcaga aggcgaccgg caaccggatc aactatcaat ccatcggatc cggtggcggc
     5581 atcaagcaga tcacggccaa gaccgttgat tttggcgcct ccgacatgcc attgaagccc
     5641 gaagttctcg acaaggacgg actgatgcag tttccgacag ttatcggtgg cgttgtgccg
     5701 gtactcaatg tggccggtat caagcccggc gaactcaagc tgaccggtcc cgtgctggct
     5761 gacatctacc tcggcaagat caacaaatgg aacgacaagg ccatcgccga actcaatcca
     5821 ggcctggcat tgccggcaga tgacatcggc gtcgtaaggc gtgctgacgg ctccggtacg
     5881 accttcattt tcagtaatta cctgtccaag gtcagcgccg aatggaaaca gaaggttggt
     5941 gaaggcacgg cagtgcaatg gccggtcggc ctcggcggca agggcaatga aggcgtatcg
     6001 gccttcgttc agcgtcttcc cggctccatc ggctacgtag agtacgcgta cgccaagcag
     6061 aacaatatga cctacgccat actcaagaac agtgccggca gttttgtcgc tcccgacgac
     6121 gccactttca aggcagcggc agcccatgcc gactggagca agtcggcatt ttacgaaatc
     6181 ctgaccgacg agccgggcaa ggactcctgg ccgattaccg gcgccacttt catcctgatg
     6241 cacaaggtgc aggacaagcc ggcgcaggca gcggaagcca tcaagttctt cgactggtca
     6301 tacaagaacg gtgacaagat ggcaaccgac ctcaactatg tcccgctccc cgacagcctg
     6361 atcaaactga tccacagctc ttggagccag ctcaaggatg cttccgggaa gcccgtcgtt
     6421 gcggccacca agtaagtcat acttatcggt ccgcgccgct tcagggcagc gcagaccttc
     6481 acccaaccct tcaatgcaaa gttctcaatg agtgcggtac tggattccaa ggcgaccggc
     6541 gcttccgcgc gcaaccacaa gctcggcgac tggttgttct tcaacctgac ccgtggcttt
     6601 gccgcgatga ctctggtatt gctggccgga attgtcgtct cgctggtcat cagctcccgt
     6661 ctgtcgatcg agaaattcgg cctgggcttt ctctggagtt cggactggaa tccgccgatg
     6721 gaagaattcg gcgcgctggt tccgatctac ggaaccctgt ccacctcgct gatcgcgctc
     6781 atcatcgcgg ttcccgtcag cttcggcatc gccctgtttc ttagcgagct gtcgcccgtg
     6841 tggctacgcc ggccgctcgg tatcgccatt gagctgcttg ctgctgtacc ttccatcgtt
     6901 tatggcatgt ggggtttgat ggttttcgca ccgatcttcg caaaatacgt gcagccggtg
     6961 ctttcaggta ccctgggcaa ggtgccgctg gtcggtgccc tgttcgccgg ggcgccgcta
     7021 ggcataggca tcctttccgc ggggatcatt ctggccatca tgatcatccc gttcatcgcc
     7081 tcggtgatgc gcgacgtgtt cgaagtcaca ccggccatgc tcaaggaatc ggcgtacggg
     7141 gtgggttgca cgacctggga agtggtatgg aatatcgttc tgccctacac acgggttggc
     7201 gtcattggcg gcatcatgct cggactgggc cgtgcgctgg gcgaaaccat ggccgtaacg
     7261 ttcatcatcg gcaattccaa cttcctctcc agcatttcgc tctatgcccc gggcaacagc
     7321 atcacatcgg ctctggccaa cgaattcggt gaagcaaccc cgggaatata cacgtcatcg
     7381 ctgattgaac tgggtctgat tctcttcttc atcacaatgg tcgtgctggt gttctcgaaa
     7441 ttgctcctgg taagcctgtc gaagcaggaa ggaacgaaga catgaggcga ctatggatct
     7501 gctgaaacgg cgcgagatca ccaacaaggt tgcgctgacc ctgtcgatgg ccgccatggt
     7561 ttttgggctg ttctggctag cgtggatatt gttcactacg ctgcaactgg gtgttgccgg
     7621 cctgtcgctt gatctgttca ccaaaatgac accaccgccc ggagctgccg aaggcggcct
     7681 ggcgaacgcc atcgtcggca gcctgtacat ggtggcgtta gcgacgctgg tgggcgcacc
     7741 gatcggcatc ctggccggca tctatcttgc agaatacgga cagcatacgt ggctcggcga
     7801 ggctacccgt ttcatcaaca gcattctcct gtcggcgccc tcgatcgtca tcggcctctt
     7861 tgtctacgcc atctatgtgg cccgggtggg caacttttca gccctttccg gcgttatcgc
     7921 cctggccctg atcgttaccc cggtggtgct atcgaccacg gaaaacatgc tgttgctggt
     7981 gcccaatacg ctacgcgagg ccgccttcgc gctgggtgcg cccaaacgcg tcgtcatcat
     8041 gaatgtgacg ctgagggctt cgaaggcagg cgtcattacc ggcatcctgc tcgccgtcgc
     8101 gcgcattgcc ggcgaaacgg cgccattgct gttcactgcg ctctccaacc agttctggag
     8161 tctcgatctc aacgaaccga tggccaacct gcccgtcacc atcttcaagt tcgccatgag
     8221 cccgttccag gactggcagg aactcgcatg ggccggcgta ctcctgatca cgctgggtgt
     8281 gcttggactg aacatcgctt cgcgcatctt cttccgcgcc aagcacaaac tgtgaaaccg
     8341 ctcaaaccat gaacgataca actcacctga tttcgctgtc cgaaaaggcg acagcccttg
     8401 aagcgcagat ggttttcaag aatctcgatt tctattatgg gaaattccat gcgctgaaag
     8461 ccatcgatct tgagatctac aaacgccacg tcacggcttt catagggcct tcgggttgcg
     8521 ggaagtcaac gctgttacgt accatgaacc ggatgtatga cctgtatccg gaacagcgtg
     8581 ccagcggcga gttgctgctc gatggaatga atatccttga tcgcaaaatg gacgtaaatg
     8641 cactgcgcgc caaggttggc atggttttcc agaaaccgac gcccttcccg atgtcgattt
     8701 acgagaatat cgccttcggg gtgcgcctga acgaaagact ttccaagggt cagatggacg
     8761 aacgcgtcga gtgggccttg cgaaaggcgg caatctggga agaaaccaag gacaaactcg
     8821 cccagagcgg aatgagcctt tccgggggac agcaacaacg gctgtgcatt gcgcgcggca
     8881 tcgcagtcaa accgaaggtg ctactgctcg acgaacccac ttcagcactc gacccgattt
     8941 ccaccatgta catcgaggag ctgatttccg aattgaaaac cgaatttact attgccatcg
     9001 tcacccacaa catgcagcag gcagcgcgca tttccgacta tacggcgtac atgtatctgg
     9061 gtgaaatggt cgagttcggc gtcacggatc aggttttcat caagccgaag aagaaggaaa
     9121 ccgaggatta catcaccggt cgtttcggct gaagcgcacg ttctgtcgct atcttgaggc
     9181 ggcccgccag acggccagtg catctttcgt ttcagccaca tcatgcacgc gcaggatgcg
     9241 ggcaccacgc tgcgcagcaa ggacggcggc cgcgaggctt ccggacaagc gctgctcaac
     9301 ggacttgccg gttatggcac ccagcatcga cttgcgggaa ataccggcca gcataggcaa
     9361 cccgtcagtg gcaagcgcgt caaagcaacg cagcagttcg agattctgct caagcgtctt
     9421 gccaaaaccg aaccccggat cgagcaccag acggtcgtag gcgaccccgc cagcgactgc
     9481 cctggcaacc cgctggccaa ggaaattgcg cacttcacca accacatcgt catagtgagg
     9541 actttgctgc atggtcaacg gctcgccctg catgtgcatc aaacaaacgg cgcaatcgct
     9601 ctcggctaca acttccagtg caccgggcat gcgcagggca tagatgtcat tgatcatcga
     9661 ggcgccccgc gcgatggcag cacgcatcac ttcgggttta tacgtatcga ctgagattgg
     9721 cacaccgcag tcggccaggg cgttcagtac cggcagcagg cggcccaatt cctcatcgag
     9781 cggggccgga atggcaccag ggcgcgacga ttcggctccg aggtcgagca aatccgcgcc
     9841 ggaatcaatc tgatgaaacg catactcgat agcgcgcttg gcatcagtgg ccaaaccatc
     9901 gccggagaag gaatccggcg tcaggttgac gatgcccatg ac
//
LOCUS       DAORPM010000071         9859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1587_length_9859_cov_13.167483, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000071 DAORPM010000000
VERSION     DAORPM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1587_length_9859_cov_13.167483"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..797)
                     /locus_tag="PLD53_05070"
     CDS             complement(42..797)
                     /locus_tag="PLD53_05070"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRC60314.1"
                     /translation="MSRLNDSELPFSLLQFYNTAAYPCSYLSGEQAISQVATPTHLIT
                     TEVYGDLVRSGFRRSGVFTYRPNCEKCKACVPVRLPIARFAPNRSQRRCLKTHATLRA
                     RELPLMYFDEHYRLYLRYQAARHIGGGMDQDNHEQYSRFLLQSRVDSRLIEFTENDVL
                     RMVSIVDVLNDGLSSVYTFYDPEIKGAGLGTYNILWQIAQCAANNWPYLYLGYWIRDS
                     RKMAYKANFRPIEGLINDEWQVFDAADPSVGNR"
     gene            complement(854..1633)
                     /gene="aat"
                     /locus_tag="PLD53_05075"
     CDS             complement(854..1633)
                     /gene="aat"
                     /locus_tag="PLD53_05075"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRC60315.1"
                     /translation="MIPWLDSANTFPPLELALREPNGLLCAGGDLSPQRIVQAYRQGI
                     FPWFSDGEPILWWSPDPRMVLFPSEFRFSRSLRRTLRANDYRIRLDGDFPAVIDACAR
                     TRRQGQAGTWITAEMQAAYCTLFERGYAHSVAVRVNDKLVGGLYGLAIGKMFYGESMF
                     SHATDASKIALAHLARFLREQEFGLIDCQMTTPHLASLGAREISRDDFLARVHELTAI
                     PPPCGRWPIDGASEGWDQETGDMQHDNRIIPPDKAQRGPSR"
     gene            complement(1630..2739)
                     /locus_tag="PLD53_05080"
     CDS             complement(1630..2739)
                     /locus_tag="PLD53_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC60316.1"
                     /translation="MAHLELDRIVQRYGSHTVVDGVSFRVDTGSIACLLGPSGCGKTT
                     LLRCIAGFEPIAEGEIRVDGVSVTRPHFRVVPERRRIGMVFQDYALFPHLTVAGNIAF
                     GLTSLGADQRQTRVGELLSTVGLDGLGRKFPHELSGGQQQRVAIARALAPRPQLILLD
                     EPFSNLDVDLRERLSLEVRSILKHAGITAVLVTHDQHEAFAMADEIGVMDEGRIQQWD
                     TPYNLYHRPGNRFIADFVGQGVFLPGTVIDNHRVEVELGLLESRLPLECMQGCNTCGK
                     GCHVEVMLRPDDVIHDDRSELQAEVVHSAFRGAEFLYTLRLASGARILSLVPSHHNHA
                     IGEHIGIRLDIDHVVAFRTDSPTTRPIAQPVLIQR"
     gene            2846..4480
                     /locus_tag="PLD53_05085"
     CDS             2846..4480
                     /locus_tag="PLD53_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRC60317.1"
                     /translation="MKLSRFNSLLVVSALIAALIGFPVASVLANIFSGGTSPTWLHLA
                     STVLSEYVGNTLLLCLGVGFGVVIVGVATAWLTAMHDFPGRRIFEWALVLPLAIPAYV
                     LAYVYTDFLQFVGPLQSGLRETFGWSRDDYWFPDVRSTGGAVTMFVFVFYPYVYLLAR
                     TAFIERASGMLDAARTLGLGPWRSFFRVSLPLARPAVVAGTTLALMETLADYGTVSYF
                     GVQTFTTGIYRAWFSFADRIAAAQLAASLLGFVVVLLVIERLSRGRARFHNTTVRHRP
                     LSGHVLKGGRAAMAVAVCGIPLLIGFLLPAGLLLRLALTEGDAQFGEHFFSLAGNSFV
                     LAGLTSAIAVALALLLAYGARASKGFVAKSFNRLVVLGYAVPGSVIAVGVLIPVTRLD
                     NWLASQWELWFGINPGLLFTGGIAALIYAYLVRFLGVALQSVSASLAKVTPSMDEVAR
                     SLGTGQGELMRRVHLPIVRGSLFTAVLLVFVDVMKELPATLVMRPFNFDTLATQAYTL
                     ASDERLAEASTASLAIVAVGVLPLIALSRQIARNRS"
     gene            complement(4537..5562)
                     /locus_tag="PLD53_05090"
     CDS             complement(4537..5562)
                     /locus_tag="PLD53_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC60318.1"
                     /translation="MYSPLARIGLVIVSCVALTGTTYADDKELNLYSARHYQTDEALY
                     SEFTRQTGIKINRIEGKEDELLERIKNEGAHSPADVLLTVDASRLATAHEMGLFAPLK
                     SKILDQRIPANLRTEDWFSFSTRARVIVYAKGSVKAEAVQNYADLAQPRLKGKLCTRS
                     GSHPYNLSLMASFIAHHGEAKAEEWARGMVANFARSPKGGDTDQIRAVAAGECEVALA
                     NTYYVARLMRSDKPDDRKVMEKVAVVWPDQQSFGTHINVSGGGMLKHAPHKDAALKFL
                     EYLASDEAQRFFADGNNEWPVVVGVKVNNPALATLGKFKADALPVGSLAMYRSKAQVI
                     FDRVGYR"
     gene            complement(5709..6221)
                     /locus_tag="PLD53_05095"
     CDS             complement(5709..6221)
                     /locus_tag="PLD53_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRC60319.1"
                     /translation="MTYIRLRSILLVAIAILLFGSGMVQATEQQQRASEEPSLLERYT
                     SNAQDLILKGLELVGIKYRRGGTDPDIGLDCSGFVQIVFKDAIGLLLPRTAREQSEVG
                     NVIDKNELKAGDLVFFNTMRRAFSHVGIYLGDNRFLHAPRSGAEIRVEDMSQSYWTKR
                     YNGARRIVER"
     gene            6460..7455
                     /locus_tag="PLD53_05100"
     CDS             6460..7455
                     /locus_tag="PLD53_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRC60320.1"
                     /translation="MSPFKAYLIKEVDGKTQADFVVMDEEQLDPGEVTIRVAYSSVNY
                     KDALAATGAGRIIRRFPCVGGIDLSGIVASSTNSRFKPGDQVIATSFDIGVAHHGGYA
                     EYARVPADWVVPLPAGLSLLEAMALGTAGFTAALGIVRMEENGLAPDKGPVIVNGATG
                     GVGGLSVDMLSKLGYKVTALTSKEAEFDYLKRLGAAEVMFRSALDLSRIRPLDKPLWA
                     GAVDNLGGDVLAWTASTMKQGGTIASIGMAAAGSFNSTVMPFILRGVCLLGIDSGYIG
                     EPYRSRVWKRLGDDLRPRHLLEMTHRIGFDELPGIFQNFLKGSARGRVVVELAGA"
     gene            7503..8645
                     /locus_tag="PLD53_05105"
     CDS             7503..8645
                     /locus_tag="PLD53_05105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRC60321.1"
                     /translation="MENPTQLPRILIVDGSRIVRAMIVKHIRDSYDFREESDGEAAWQ
                     ALVLDQSIRLVICSLSLPVLDGDGLLVRVRSSRLPRIAQMPMLMISGDNDEVLARAKT
                     HGASDFISRGTGGAELLGRIESLLKLSEAQNQLKENLEQHVQNPETGLFTRKYVELQA
                     AQAMSHAMRHNSEVSAIVMGFDNVGAIREEHGADVLKLLQQRFIAMLVSKVRKEDSLG
                     HFAGSQLVVISPGTPYPACESFGNRLRDAIRVSNIAVHGQRLNLSVSVGVSNSPVDGV
                     SSAGALIELAGSRLKVAQQSGGNRVIACNVQPLTATVAPRLEHAIALIKSGHESEVVP
                     HLAALGSQVLPLLKVLERELKLDLPMADIEKCLLDLAQKGKDAGQD"
     gene            8691..>9859
                     /locus_tag="PLD53_05110"
     CDS             8691..>9859
                     /locus_tag="PLD53_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC60322.1"
                     /translation="MTTIKEFHKQSIDNPDAFWAEEAKLIDWHKPFTQTLDFSRPPFA
                     KWFVGGLTNICYNAVDRHAAKRPNDRALVFISTETDQEVVYSFADLKKEVMRMAAVMQ
                     SLGVGKGDRVLIYMPMIAEATFAMLACTRIGAIHSVVFGGFASGSLATRIDDATPKLI
                     VSSDAGMRAGKAVPYKNLLDEAISLAQHKPAKVLMVDRGIDKGFNKVEGRDVDYATLR
                     AKFMDAEVPITWLESSEPSYILYTSGTTGKPKGVQRDTGGYAVALASSIKNIYCGEEG
                     ETYFSTSDIGWVVGHSYIVYGPLIAGMCTIMYEGTPLRPDPGIWWQIVEKYKVNVMFS
                     APTAARVLKKHDSSYMHKYDLSSLKHLFLAGEPLDQPTHEWIMGELGLPVIDNYW"
BASE COUNT         1923 a         2653 c         3056 g         2227 t
ORIGIN      
        1 gcacggctta tccctcttct tattgataga cgcttgccag gctagcggtt tccgacgctc
       61 gggtcggcag cgtcgaacac ttgccattcg tcgttgatca gtccctcgat gggccggaag
      121 ttggccttgt atgccatctt tcgactgtcg cggatccagt agccaaggta cagatagggc
      181 cagttgttgg cagcgcactg ggcgatttgc caaaggatat tgtaggtgcc gaggccggcg
      241 cctttgattt cggggtcgta gaaggtgtag acggaggaaa ggccgtcgtt cagcacgtcg
      301 acgatgctga ccatgcgcaa tacgtcgttc tcggtgaatt cgatcagccg gctgtcgacg
      361 cggctctgca gcaggaaacg cgagtactgc tcatgattgt cctgatccat gccgccgcca
      421 atatgccgcg cggcctggta gcgcaagtag agccggtagt gctcgtcgaa atacatgagc
      481 gggagttcac gggcgcgcag agtagcgtgc gttttgaggc agcgccgctg gctgcggttg
      541 ggggcgaagc gcgcaatcgg cagccgtact ggaacgcagg ccttgcattt ctcgcagttg
      601 gggcggtagg taaagacgcc gctgcgacga aatccgctgc gtacgaggtc gccgtagact
      661 tcggtggtga tcagatgggt tggcgtggcg acctgcgaaa tggcctgctc gccggacagg
      721 taactgcacg ggtaggcggc cgtgttgtaa aactgcaaga gcgagaaggg aagttcggaa
      781 tcgttgaggc gagacatcgg ggttcttcat ccggttgaca tgggaaaact ctgccaccaa
      841 atcaacgtcg cgtctaccgc gatgggcctc gctgggcctt gtcgggcggg atgatccgat
      901 tgtcatgttg catgtcgcca gtttcctggt cccaaccctc gctcgcaccg tcgatcggcc
      961 aacggccaca cggcgggggg atggcggtca gctcgtgtac ccgggccagg aagtcgtcgc
     1021 gagatatttc gcgtgcgccc agcgaggcaa gatgcggcgt cgtcatctgg caatcgatca
     1081 ggccgaattc ttgttcgcgg agaaagcgtg caaggtgcgc gagtgcgatc ttcgaagcgt
     1141 cggttgcgtg cgagaacatg gattctccgt agaacatctt gccgatggcc agcccataca
     1201 ggccgccgac cagtttgtca tttacccgga ctgcgacgga gtgggcatag ccacgctcaa
     1261 ataatgtgca ataggccgcc tgcatctcgg cggtgatcca ggtccccgcc tggccctggc
     1321 gtcgtgtacg ggcgcaggcg tcgatgactg ccgggaaatc accgtcgagc ctgatccggt
     1381 aatcgttcgc gcgcagagtg cggcgcagtg agcgtgaaaa ccggaactcg gagggaaaga
     1441 gaaccatgcg tgggtcggga ctccaccaga ggatgggttc gccatccgag aaccagggga
     1501 agatgccctg ccggtaggct tggacaatgc gttgcggaga aaggtcgccg cctgcgcaga
     1561 gcagaccgtt cggctctctg agagcgagtt cgagtggagg gaaggtgttg gcgctgtcga
     1621 gccagggaat catcgctgga tcaatacggg ctgggcgatg gggcgcgtcg ttgggctatc
     1681 cgtccggaag gcgacgacat ggtcgatgtc gaggcggatt ccgatgtgtt cgccaatggc
     1741 atgattgtgg tgcgagggta cgagcgaaag aattcttgcc ccgcttgcca gtcggagggt
     1801 gtacaggaat tcggcgccac ggaaggcgct gtgcacgact tcggcctgca attcactgcg
     1861 gtcgtcgtga ataacgtcgt ccggacgcag catcacttca acatggcaac ccttgccgca
     1921 ggtattgcag ccctgcatgc attcaagcgg caggcgcgat tcgagcagac cgagttcgac
     1981 ctcgacacga tggttgtcga tgaccgtacc aggcaggaat acaccctgac ccacgaagtc
     2041 ggcgataaag cggttgcctg gccggtgata gaggttgtag ggcgtgtccc actgctggat
     2101 tctgccctca tccatgacgc cgatttcgtc ggccatggcg aatgcttcat gctgatcgtg
     2161 cgtaacaagc acggcggtga tgcctgcatg cttgagaata ctgcgtacct caagcgacag
     2221 gcgctcgcgc aggtcgacat ccagattgga gaagggctcg tcgagcagga tcagttgcgg
     2281 gcgcggggcg agggcacggg ctatcgcgac acgctgctgc tgtccgccgg agagttcgtg
     2341 tggaaacttt ctacccaggc cgtcaaggcc aacggtggat agcagttcac cgacacgcgt
     2401 ctgtcgctgg tccgcgccga gtgaggtcaa accgaacgca atgttgccgg cgaccgtgag
     2461 gtgagggaag agggcgtaat cctgaaaaac catgccgatg cgtctccgct cgggcactac
     2521 acggaagtgg gggcgggtga ccgaaactcc gtcaacccga atctcacctt cggcgatggg
     2581 ctcgaatccg gcgatgcagc gcagcagggt cgttttgcca cagccggaag ggccaagcag
     2641 gcaggcgatg ctgccggtat caacacgaaa gctgacgcca tcgacgacgg tgtgcgatcc
     2701 gtagcgctga acgatgcggt caagttcgag atgagccatc aggagaattt ggaaacaggt
     2761 gcaggttgaa gcagcggaaa aaagcggaga ggatcagagc tgcccgaggc ggtcaattat
     2821 aattcgcttt cttttttggt cggccatgaa actctcccgt ttcaattcgc tcctcgtcgt
     2881 ttccgcattg attgctgcct tgatcggttt tccggtggcc agcgttcttg ccaacatatt
     2941 cagtggcggg accagtccga cttggcttca tctggcctca acggtcttat cggagtatgt
     3001 cggcaacacg ctcttgctgt gtctgggcgt gggattcggt gtcgtcatcg tcggcgtcgc
     3061 cacggcctgg ctgaccgcga tgcacgattt tccgggacgg cgcatattcg aatgggcgct
     3121 ggtcttgccg cttgcgattc cagcctatgt gctggcctac gtctatacag actttcttca
     3181 attcgtcggc ccgctgcaaa gcggactgcg tgaaaccttc ggctggagcc gcgacgacta
     3241 ttggttcccg gacgtgcgca gtacgggcgg tgccgtgacg atgttcgtat tcgtgtttta
     3301 tccctatgtc tatctgttgg cacgcacggc cttcatcgag cgtgccagcg gtatgctgga
     3361 tgctgcgcgc acgctcgggc tcggtccgtg gcgcagcttc ttccgggtgt cgctgccgct
     3421 ggcgcggccg gcagtggttg ctggcacaac cttggcgctg atggagacac tggccgacta
     3481 tggcacggtg tcctacttcg gcgtgcagac ttttacgacc ggaatttatc gtgcgtggtt
     3541 ctcatttgcg gaccggatcg cggcagctca actagcggct tcgcttcttg gttttgtcgt
     3601 cgtactgctt gtaatcgagc ggctttcgcg cggtcgggcg aggtttcaca atacgaccgt
     3661 tcgccatcgt ccgctgtccg gccatgtgct gaagggcggt cgtgcggcga tggcggtcgc
     3721 ggtatgcggc attccgttgt tgatcggatt cctcctgccc gccggcttgc tgttgcgact
     3781 ggcgttgacc gagggtgacg cacagttcgg cgagcatttc ttttcactgg ccggaaacag
     3841 tttcgtgctg gccgggctga cgtcggcgat tgccgttgcc ctggctttgc tgctggccta
     3901 tggtgcccgc gcgtcgaagg ggtttgtcgc gaaatcgttt aaccgtctgg ttgtcctcgg
     3961 ctacgccgtt cccggttcgg tgatcgccgt cggcgtactc atcccggtaa cgcgactgga
     4021 taactggctg gcttcacaat gggaactctg gttcggaatc aatccggggc tgctctttac
     4081 gggcggcatc gcggcattga tctatgctta tctggtgcgc ttccttggcg tggcattgca
     4141 gtcggtcagc gcaagtctgg ccaaggtgac gccgagcatg gacgaggtgg cgcgaagtct
     4201 gggcaccggt cagggagaac tcatgcggcg ggtgcatctt ccgatcgtgc gcggcagctt
     4261 attcaccgct gtcctgctgg tcttcgtcga tgtaatgaag gagctgcccg caacgctggt
     4321 gatgcgaccc ttcaacttcg ataccctggc cacccaggct tacactctgg cttcggatga
     4381 acgtctggcc gaagcttcga ccgcgtccct ggcgattgtt gcggtcggag tgctaccgtt
     4441 gatagcgctg tcgcggcaga tcgccaggaa cagaagctga atgaagtgcc cacggcgaac
     4501 atggcggcaa gccgcgatcg ccgtggttgg atctttctat cgataaccca cgcgatcaaa
     4561 gatcacttgc gctttcgatc ggtacatcgc cagcgagcca accggtagcg cgtcagcctt
     4621 gaacttgcca agcgtcgcca gcgccgggtt attaaccttg accccgacaa ctaccggcca
     4681 ttcgttgttg ccgtcggcaa agaagcgctg cgcttcgtcg ctcgccagat actccaggaa
     4741 cttgagcgcg gcatccttgt gtggtgcgtg cttcagcatc cctccaccgg acacgttgat
     4801 gtgtgttcca aaactctgct gatccggcca gacgaccgcc accttttcca tcactttcct
     4861 gtcatcgggt ttgtccgagc gcatcagacg agccacgtag taggtattgg caagcgccac
     4921 ttcacattca ccagcggcca ccgcccggat ctggtccgta tcgccgccct tgggggaccg
     4981 tgcgaagttg gcgaccatgc ctcttgccca ctcctcggct ttggcttcgc catggtgcgc
     5041 gatgaaggac gccatcaggg aaaggttgta ggggtgcgag ccggatcgag tgcacaactt
     5101 gcccttgagt ctcggctgag ccaggtcggc gtagttctga acagcctcgg ctttgacaga
     5161 acctttggcg taaacgatga cccgggcgcg cgtcgagaag gaaaaccagt cttcggtacg
     5221 caggttcgca ggaatgcgct ggtcgaggat tttcgactta agcggagcga agagccccat
     5281 ctcatgtgcg gtcgcgagac gtgaggcatc gacggtcaga agaacatcgg ccgggctgtg
     5341 ggcgccttca ttcttgatgc gttcgagcag ttcgtcttcc ttcccttcga tgcggttgat
     5401 cttgattccc gtctgccggg tgaattcgga ataaagtgct tcgtcggtct ggtagtggcg
     5461 agcagagtag aggttcagtt ccttgtcgtc ggcgtaagtg gtaccagtta atgcgacgca
     5521 agagacgatg acaaggccga tgcgagctaa gggggaatac atgatggtta ctcctgatat
     5581 taaaaaagaa cgcttctcaa taaggaggaa ttatagcgag tactccccaa tgtgtaaatg
     5641 atacgtatta tcatcaaaca gtaaaaaaat ggacgcctgc gcgtccttcg gagttttgtg
     5701 ccgtcatctc agcgttcaac gatgcggcgt gctccgttgt agcgctttgt ccagtagctt
     5761 tggctcatgt cctcgacacg tatttctgcg ccgctacgcg gtgcgtgcag gaagcggttg
     5821 tctcccaggt aaatgccgac atgggagaag gcgcgacgca tggtattgaa gaaaacaagg
     5881 tcacccgcct tgagttcgtt cttgtcgata acgttgccga cctcgctttg ttcacgcgct
     5941 gtgcgtggca gaagcaggcc gatggcatcc ttgaaaacga tttgaacaaa tccgctgcag
     6001 tccagcccga tatcggggtc cgtgccgccc cgccgatact tgattccgac cagctcaagg
     6061 cccttgagga tcagatcctg cgcattgctc gtgtagcgtt ccagcagtga gggctcctca
     6121 ctggctcgtt gctgctgctc ggtcgcctgg accatgccag agccgaatag caagattgca
     6181 atggcgacaa gcaggattga gcgtagtctt atataagtca tgggagggca atctaaaaga
     6241 aaaacttccg ggtgtaaacc ctttcgtcca cgacttccgc tgtaggttgc cgggcgtcac
     6301 cgcgtaacca catgattaat agtgtctttg tgattccctc tttagccgcg tatatggata
     6361 ctggtagaag ggttttgtgc ttataatcga caaatggctg ttggcgttgc cgacttcggc
     6421 cgtgtcgcaa gaaatctccg attccagcaa ggagcgctca tgagcccctt caaggcttat
     6481 ctgatcaaag aagtggatgg aaaaacgcag gctgatttcg tcgtcatgga tgaggaacaa
     6541 cttgatcctg gcgaggtgac gatcagggtt gcttattcca gcgtcaatta caaggatgct
     6601 ttggccgcga ctggtgccgg gcgtatcatt cgtcggtttc cttgcgttgg cggcatcgac
     6661 ctctcgggga ttgtcgcaag cagcacgaat tcgcgattca agcccggcga tcaggtgatc
     6721 gcaaccagtt ttgatatcgg cgttgcacac catggcggtt atgcggagta cgcgcgcgtt
     6781 cccgccgact gggtagtccc tctgcctgcc ggcctttcac ttcttgaagc gatggctctc
     6841 ggtacagcag gctttaccgc tgcgctgggg attgttcgta tggaggaaaa tggcctcgcg
     6901 cccgacaaag gaccagtgat cgtgaacggg gcgaccggcg gcgtcggtgg attgtccgtg
     6961 gatatgctgt ccaaattggg ctataaggtg acggcgttga ccagcaagga agccgagttt
     7021 gactatctca aacgcttggg tgccgccgag gtcatgttcc gctcggcgct cgatctatcg
     7081 cgcatccgtc cgctcgacaa accgctctgg gccggcgcgg tggacaatct cggtggtgat
     7141 gttctggcct ggacggccag tacgatgaag cagggtggca cgatagccag catcggcatg
     7201 gctgcggctg gctcgttcaa ttcgacggtc atgcccttca tattgcgtgg cgtatgtttg
     7261 ctgggtatcg attcgggtta cattggcgag ccctaccgta gccgcgtctg gaagcgcctg
     7321 ggagacgatc tgcgaccgcg tcacttactt gaaatgacgc accggatcgg cttcgatgaa
     7381 ttgccaggca tctttcagaa tttcctgaaa ggaagcgcgc gtggtcgcgt cgtcgtcgaa
     7441 ttggctggcg cctagtctgt atccggatgc atagctcaag gtaattgcga actgaattac
     7501 gcatggaaaa tccaacccag ttgccacgta tattgattgt cgatgggtcg cgtatagtgc
     7561 gcgcgatgat cgtcaagcat atccgcgatt cttacgattt tcgtgaggaa tcggacgggg
     7621 aggcggcttg gcaggctctc gttcttgatc agtccattcg gctggtgatt tgttcgcttt
     7681 ccctgcccgt gcttgacggc gatggtttgc tggtccgtgt gcgctcctcg cgcctgcccc
     7741 gtattgcgca gatgccgatg ctgatgatat ctggcgacaa tgatgaggtt cttgctcgtg
     7801 ccaagacaca cggcgcgtct gatttcatca gccgaggtac tggcggcgcc gaactgctgg
     7861 ggcggatcga atcgctgctc aagctttctg aagcgcagaa ccagctcaag gaaaaccttg
     7921 agcagcatgt acagaatccg gaaacgggcc tgtttacgcg caagtacgtc gagttgcagg
     7981 ctgctcaggc gatgtcgcac gccatgcggc acaacagtga agtcagtgcg atcgtgatgg
     8041 gtttcgacaa tgtcggcgca atacgtgagg aacatggagc tgacgtattg aagctgttgc
     8101 agcaacgatt tatcgccatg ctggtcagca aggtacgcaa ggaagacagt ctgggccatt
     8161 ttgcgggcag ccaactggtg gtaatctcgc caggaacgcc ctatcctgca tgcgagtcct
     8221 tcggcaatcg gctgcgtgat gccatccgcg tatccaatat cgcggtacac ggtcagcgtc
     8281 tgaacttgtc tgttagtgtc ggtgtcagca attcccctgt agacggggta tcctccgcag
     8341 gggcgctcat cgaattggcc gggtcgcgtt tgaaggtagc gcagcaatcc ggtggtaatc
     8401 gcgttattgc ctgcaatgtt cagccattga ctgcgacagt tgcgccacgg cttgagcatg
     8461 ccatcgcctt gatcaaatcc gggcatgaga gtgaggtggt gccgcacctg gccgcattgg
     8521 gcagccaggt gttgccacta ttgaaggtgt tggaaaggga gttgaagttg gatctgccca
     8581 tggcagatat tgagaagtgc ttgcttgacc tggcgcagaa gggaaaagac gccgggcagg
     8641 attagttacc attagtgcag gttgttcagt atcagcaaaa aggaaaactt atgacgacaa
     8701 tcaaggaatt tcacaaacaa tccatcgaca atccggatgc tttctgggct gaggaagcca
     8761 agctgattga ctggcacaag ccgtttactc agacactcga cttttcgagg cctccgtttg
     8821 ccaagtggtt tgttggtggc ctgacgaata tttgctacaa cgctgtggat cgtcatgcgg
     8881 ccaagcgccc gaacgaccgt gcgctggtct tcatctcgac cgaaaccgac caggaagttg
     8941 tttactcctt cgccgatctg aagaaagagg tcatgcgcat ggcggcagtc atgcagagcc
     9001 ttggcgttgg caagggcgac cgcgttctga tctacatgcc gatgatcgcc gaagccacgt
     9061 tcgccatgct ggcctgcacg cgcattggcg ccattcactc ggtggtgttc ggcggcttcg
     9121 cctccggatc gctggctacg cgaattgatg acgcgacgcc gaagctgatc gtttcctcgg
     9181 atgccggcat gcgtgctggc aaggcggttc cgtacaagaa cctcctcgat gaagccattt
     9241 cactggctca acacaagccg gccaaggtat tgatggtgga tcgtggcatc gacaaggggt
     9301 tcaacaaggt cgaaggtcgc gacgtcgact acgcgacctt gcgtgcgaag ttcatggatg
     9361 ccgaagttcc gatcacctgg ctggaatctt ccgaaccttc ctacattctc tatacgtccg
     9421 gcacaaccgg caagccgaag ggtgtgcagc gcgatacggg aggttatgcc gttgcgctgg
     9481 cctcttcgat caagaacatc tattgcggcg aggaaggcga gacttatttc tcaacctctg
     9541 atatcggttg ggtcgttggt cactcctaca tcgtttacgg tccgctgatt gccggcatgt
     9601 gcaccatcat gtacgaggga acaccgttgc gtcccgatcc cggtatctgg tggcagatcg
     9661 tcgagaagta caaggtcaac gtcatgttct cggcgccgac ggcggctcgc gtcctgaaga
     9721 agcacgacag ctcgtacatg cacaaatacg atctgtcgtc cctgaagcac ttgttcctcg
     9781 cgggtgagcc gcttgatcaa ccgacgcacg agtggatcat gggcgaactc ggcttgccgg
     9841 tgatcgacaa ctactggca
//
LOCUS       DAORPM010000072         9855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1590_length_9855_cov_9.894694, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000072 DAORPM010000000
VERSION     DAORPM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_9855_cov_9.894694"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /gene="mutS"
                     /locus_tag="PLD53_05115"
     CDS             complement(<1..2272)
                     /gene="mutS"
                     /locus_tag="PLD53_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRC60323.1"
                     /translation="MDSPSGLNDLGKHTPMMQQYLSLKAQHPHTLLFYRMGDFYELFF
                     DDAEKAARLLDITLTTRGQSAGQPIKMAGVPYHSLEPYLAKLVKLGESVVICEQIGDP
                     ATSKGPVERAVARIVTPGTLTDAALLDDKRDVLLLALTLTGKTAGLAWLNLASGAFRI
                     AEVATDKLSATLERIRPAEIVVAESLDLPFVPDAALTRQPDWHFDSEAATRELCAHLH
                     TASLSGFGADGLRFGVAAAGALLRYAQLTQTRALPHVQALVVERDDTYLGLDTATRRN
                     LELTETLRGQPSPTLCSLLDTCVTTMGARALRHALHHPLRDSAIPAARHAAIEALLDG
                     NNGCGQRLREVRQALRGFADIERIAGRIALYSARPRDLSGLRDSLQRLAELRTALDGT
                     PAPLLAELLEALTTPAATLDLLIRAVLPEPAALIRDGGVIANGFDADLDELRALNDNC
                     GAFLIDLEARERERSGIASLKVEYNRVHGFYIEVTHANATKVPEDYRRRQTLKNAERY
                     ITPELKAFEDKALSAQERALAREKILYEGVLADLQSELPQLQRIARAVAMCDLLACFA
                     DVAQTRNYCRPLFSTEPGLLIEGGRHPVVESELAGGETFIANDTKLGDSAGGRRLLLI
                     TGPNMGGKSTYMRQTALIALMAHIGCFVPAKRAVLGPLDQIFTRIGASDDLASGRSTF
                     MVEMTESAAILHHASEHSLVLMDEVGRGTSTFDGMALAFAICRHLLEKNRCLTLFATH
                     YFELTLLANEYPDLANV"
     gene            2468..2890
                     /locus_tag="PLD53_05120"
     CDS             2468..2890
                     /locus_tag="PLD53_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HRC60324.1"
                     /translation="MANILRYTAGEDPIDDLFRGFFMRPVRFEGQQDIQIKVEVSEDD
                     KAYTVHAEIPGVKKEDIRVTVDGNQVAISAEVKNEKEVKEGSKVLRSERYYGTVSRSF
                     ALGQEVDDSAVVAKYVDGVLELNLPKRAIEKAKQISIQ"
     gene            complement(3032..>4915)
                     /locus_tag="PLD53_05125"
     CDS             complement(3032..>4915)
                     /locus_tag="PLD53_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC60325.1"
                     /translation="ADEPTDRNSEIHEFFLDNGSPLMEVRRRKKSGETFRANLSLEHV
                     LDKNNEAIGLVAHLSEIADQLSTAEKQRLHARIIENSEEGILITDSKERIVSVNPAFT
                     RITGYSAGEAIGKTPDLLRSGVHSADFRAEVLAAMHGAGAWQGEIIGRRKNGELFPQS
                     VCIGVARDRDGVITHAFSIFSDISVLRAAEQRMQQIVNYDSLTGLPNRTLFYQLVEQA
                     LISARRNSEYGAMLVVDLNRFTSINDSLGHEVGDELLRQVGQRFQAALRDGDILSRFG
                     ADEYVVSLVSIHKREQSGLVAKKILDSLDKPFVIDSHLLHVGASIGVALYPDDGLNAV
                     ALHRFAYAAMQRLQRNNESGFLFYSPEMNARAKDQWLLEGELRTALTGHELVLHYQPK
                     VSLRSGRIVGAEALIRWLHPSKGLIPPYKFIPLAEETGLIVEVGTWVLEEACRQIRSW
                     LDAGLEPIPIAVNLSARQFNNELPERLLVPVERFRVPCELLRLEITESLLVRGPEKVI
                     PIMNKLVAMGFNLSLDDFGTGYSSLAYLKKFPITTLKIDRAFVVGVPGDENDCAIAQA
                     IVTMGKQLRQEIVAEGVETQAQMAFLRRLGCDQLQGYLFSPPVAADAFEEMVRKGQRL
                     PLD"
     assembly_gap    4916..5015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5037..6029)
                     /locus_tag="PLD53_05130"
     CDS             complement(5037..6029)
                     /locus_tag="PLD53_05130"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRC60326.1"
                     /translation="MSLPPLLSFTLLADRNLNPAAVMLRVGKGDMSALLPLLEGEDFS
                     TFSATFPCLFDASLAERLPPELLTALQNAGCQIMREEQSYNSDEQTKPVLPTTAEWLT
                     GDWYCAPPARQSASQATSRTVSLKLLQLVATDADTCEIEAIFRQDPVLAFHLLRLVNS
                     LGVGVGRHISSFSQAILILGRQQLKRWLNLMLFAASRDDHRSAMLLARVAVRARTMEL
                     LAKAGGLDRSVQEQAFMAGMFSLLGTLFGLPLVDILKPLRLSDSLTNAVLKHEGELGQ
                     LLLAVQSTECTEEPVLVRILSGFGLSIADYNPLSLEAYQWMLGVIHEQKDAVDA"
     gene            6376..7491
                     /gene="zapE"
                     /locus_tag="PLD53_05135"
     CDS             6376..7491
                     /gene="zapE"
                     /locus_tag="PLD53_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HRC60327.1"
                     /translation="MPHRVLKVPERGMLDAYEAVLSERGFSADAAQRAAAERLQLLYY
                     QLLSFKVGRKSTLRRVFSPPVVPRGVYFWGGVGRGKSFLMDCFYDSVPYVRKRRIHFH
                     AFMHGVHQQLNVFKGESDPLTKVAERIADECRLICFDEFHVSDIADAMILRRLLEALF
                     ERGVVFVMTSNYPPNRLYPNGLQRENFLPAIALIESNVDVVEVDSGVDYRLRTLEQVE
                     IFHYPADANAESKMAGYFSSIAGGEGAQGGSVDVLGRAIPTLRQGTGGVIWFDFKSLC
                     GGPRSQNDYLELSRSYHTILLSGVPRMTASMSSEARRFTWLVDVLYDHKVKLIVTADC
                     EPELLYVEGTQASEFFRTVSRLTEMRSREYLALPHLS"
     gene            7537..8403
                     /locus_tag="PLD53_05140"
     CDS             7537..8403
                     /locus_tag="PLD53_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60328.1"
                     /translation="MHRLFLLAGLGLSGLSFAQQVMEIIPLKYRTVEQVLPSLQVLIE
                     PGGALSGMNNQLIVRSSGKNIAQIRQALSAIDLPARRLMIYVSQNRDTESRQQGAEVS
                     GQVGFGNNVRIIQPSSGMQGSTRIDVRSGDSDVNYRINAQAHDSRVTSDMAATQSVQV
                     VDGGQALIRVGQSLPMPLRQVVRGPGGVTVTDSVVYRDIGQGFYAAPRLVGNQVTLEI
                     SPQFDTPGNQGYGSVNSQRVSTTISGRLGEWIELGGSGQQMAGNVRGNLSSATQELRD
                     YRSVWLRVEELP"
     gene            complement(8419..9339)
                     /locus_tag="PLD53_05145"
     CDS             complement(8419..9339)
                     /locus_tag="PLD53_05145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRC60329.1"
                     /translation="MNPAEYFSPLPNTHAKDEEIKRYAGLLDSLGIGLVVFYPDASPC
                     LSNKVASKLIGNTPPNWVNDSGQIICADELPLKRALRTSKPVFNCVMALTNDQERKNW
                     LSVNALPVFSDNGGVRRVLLTLTDINKEKDLQSEVKKLSVRDQLTGVYNQRHVMYLLE
                     NEIHRARRYGTPFTLAQVDIDHFLHFCEKHGPEPGERVLSGIGKLLTETMREIDIAGR
                     IGDDEFLLVLPNVSLKEAMIGLERLRMLIETQEFVSAGLRITVSGGITEYTGENSETL
                     LERAKSLLIHAREAGRNRFCLDGDILLLEP"
     gene            complement(9448..>9855)
                     /locus_tag="PLD53_05150"
     CDS             complement(9448..>9855)
                     /locus_tag="PLD53_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRC60330.1"
                     /translation="LRMDRICENTTKVAQFLKSHAKVSWVNYAGLPDHKDHALVQKYM
                     DGLASGILTFGVKGGFAAGGRFQDALQLFTRLVNIGDAKSLACHPASTTHRQLSPEEL
                     VKAGVSEDMIRLSVGIEHIDDLIADLDQALTAA"
BASE COUNT         2070 a         2633 c         2892 g         2160 t
ORIGIN      
        1 gcacgttggc caggtccggg tactcattgg ccagcaaggt caactcgaag tagtgggtgg
       61 caaacagcgt caggcagcgg ttcttttcga gcagatggcg gcagatggcg aacgccaatg
      121 ccatgccgtc gaaagtcgag gtgccgcggc cgacctcgtc catcagcacc aggctgtgtt
      181 cgctggcatg gtgcaggatc gcggccgatt cggtcatctc gaccatgaag gtcgagcgcc
      241 cggaagcaag atcgtcggat gccccgatgc gcgtgaaaat ctggtcgagc gggccaagaa
      301 ccgcgcgctt cgctggaacg aagcagccga tatgcgccat cagggcaatc agtgccgtct
      361 gtcgcatgta ggtcgatttg ccacccatgt tcgggccggt gatgagcaac aggcgccgcc
      421 ctccggcgct gtctccgagc ttcgtgtcgt tggcgatgaa tgtttcgccg ccagccaatt
      481 cgctttcgac gactggatgg cgaccccctt cgatcaacag gcctggctcg gtagaaaaca
      541 gcggccggca atagttgcgc gtctgtgcaa catcggcgaa gcaggccagt agatcgcaca
      601 ttgccacggc gcgggcgatg cgctgcagct gcggcaactc ggactgcaag tccgccagca
      661 cgccttcgta gaggatcttc tcgcgagcca acgcgcgttc ttgcgccgac aacgccttat
      721 cctcgaaggc cttcaattcg ggtgtgatgt agcgctcagc attcttcagc gtctgtcgac
      781 ggcgatagtc ttccggcacc ttggtcgcgt tggcatgggt gacttcgatg tagaacccat
      841 gcacacggtt gtattcgacc ttgagactgg cgatgccgct acgctcgcgt tcgcgtgcct
      901 caagatcgat gaggaaggca ccgcagttgt cgttgagcgc gcgcagctca tcaaggtcgg
      961 cgtcaaagcc gttggcgatc actccgccat cgcggatcag cgcggcgggt tcgggaagaa
     1021 cggcccggat cagcaggtcc agtgtggcgg ctggtgtggt gagcgcttcg agcaattcgg
     1081 ccaggagcgg tgccggtgta ccatccaatg ctgtgcgcaa ttccgccagc ctttgcagac
     1141 tgtcgcgcag accggaaagg tcgcgcggtc gagcgctgta aagcgcgatc cggccggcga
     1201 tacgttcaat gtcggcaaac cctcgcagcg cctggcgaac ctcgcgcaac ctttgaccac
     1261 agccattgtt tccgtcgagc agcgcttcga ttgccgcatg gcgtgcggcg ggaatcgccg
     1321 agtcgcgcag cgggtggtgc aaggcatggc gcagcgcgcg agcgcccatc gtggtgacgc
     1381 aagtgtcaag caggctgcac agcgttggtg acggctggcc gcgcagggtt tcagtgagtt
     1441 caaggttacg ccgcgtggcc gtgtcgagac cgagataggt gtcatcacgc tcgacgacaa
     1501 gggcttgcac gtggggcagg gcgcgtgttt gcgtcaattg tgcatagcgc agcagggcac
     1561 cggctgcagc gacgccgaaa cgcagtccgt cggcgccaaa accggacagc gaagcggtgt
     1621 ggagatgggc gcagagctcg cgcgtcgccg cctcgctgtc gaaatgccaa tccggctggc
     1681 gggtgagcgc ggcgtcagga acaaacggca ggtcgaggct ttcagcaaca acgatttccg
     1741 ccgggcggat gcgctccagc gtggccgaca gcttgtcggt ggcaacctcg gcgatgcgga
     1801 aggcaccgct ggccagattc agccaggcca gtccggcagt tttaccggtc agggtcagtg
     1861 ccagcaacag aacatcgcgc ttatcgtcaa gcaaagccgc gtcggtgagc gtgccgggcg
     1921 tgacgatgcg ggctaccgcg cgctcaaccg gccccttgct ggttgccgga tcgccgatct
     1981 gctcgcagat caccaccgat tcgccgagct tgaccagttt cgcaagataa ggctcaagag
     2041 aatgataggg aactccggcc atcttgattg gctgtccggc cgactgcccc cgcgtggtca
     2101 aggtgatatc gagcagtcgc gcggctttct cggcatcgtc gaaaaacagc tcgtaaaaat
     2161 cgcccatccg ataaaaaagc agcgtgtgcg gatgctgtgc cttgaggctc agatactgct
     2221 gcatcatcgg agtgtgcttt cccaagtcat tgaggccgct tggagaatcc atgtcaatac
     2281 ttgtcattca aaaacttgag agcacattgg cgatatgttg tggcgcgctg gcgcacttca
     2341 aaattgagca cgcgaaggaa cagaatagcc attttattcg atctgtcaga accctcttga
     2401 aatcattgct gtgatacctc atgtagtagc cgtacgggga taacccggtt ttccacagga
     2461 gagtgagatg gcaaacattt tgcgttacac cgccggcgaa gatccaatcg acgatctttt
     2521 tcgcggcttt tttatgcgcc cggtacgctt tgaagggcaa caggatatcc agatcaaggt
     2581 tgaagtgagt gaagacgaca aggcctacac cgttcatgcc gagattcccg gcgtaaagaa
     2641 ggaggatatc cgggtcacgg tcgacggcaa tcaggtcgcc atcagtgccg aagtaaaaaa
     2701 tgaaaaggaa gtgaaggaag gcagcaaagt gctgcgtagc gaacggtact acggcacggt
     2761 gtcgcgttca ttcgcgctgg ggcaggaagt tgacgactca gccgtggtgg cgaaatatgt
     2821 tgatggcgtt ctggaactga atttgccgaa acgcgcgatc gagaaagcaa aacagatttc
     2881 cattcaatag cttgaaccct gcagggcaga ggatactgtc atcggctgat gtgtagaagt
     2941 tgcgacagct gctggcaagg cggttttgcg cgaaccgcat cctctgcgcc tgtccagagt
     3001 ttacagcatg aacctggggc gtgtcgctgt atcagtcaag cggcaggcgt tgacctttcc
     3061 tgaccatctc ttcaaatgcg tcggcggcaa cgggtgggct gaacagatag ccttgcagct
     3121 ggtcgcatcc caatctgcgc aggaaggcca tttgcgcttg cgtctcgacg ccttctgcga
     3181 caatttcctg ccgtagctgt ttgcccatgg tgacgatggc ttgggcgatg gcgcagtcgt
     3241 tttcgtcgcc cggaacgcca acgacgaacg cgcgatcgat cttcagagtc gtaatcggaa
     3301 atttcttcag ataagccaga ctggagtagc cggtgccgaa atcgtccagt gagagattga
     3361 atcccatggc tacaagtttg ttcatgatcg gaatgacttt ttccggtccg cgaacgagca
     3421 aactctcggt gatctccagg cggagtaatt cacaaggcac cctgaatcgt tcaaccggaa
     3481 ccagcaggcg ctccgggagc tcattgttga actgccgggc cgagaggttg acggcgatcg
     3541 ggataggttc cagtccggca tccagccagc ttcggatctg tcgacaagcc tcttcgagta
     3601 cccaggtgcc gacttcgacg atcaggcccg tctcttcggc cagtggaatg aatttgtaag
     3661 gcgggatcaa tccctttgac ggatgcaacc agcggatgag tgcttcggcg ccaacgatcc
     3721 gtccactgcg caggctgact ttgggttgat agtgcagcac gagttcatgg ccggtcagcg
     3781 ccgttcgcaa ttcgccttcg agcagccact gatccttggc gcgtgcgttc atttccgggc
     3841 tgtagaacag gaagccggat tcgttattcc gttgcaggcg ctgcattgcg gcataggcga
     3901 aacggtgcag agctaccgca ttgagaccgt cgtcagggta cagcgctact ccgatactgg
     3961 ctccaacgtg caggaggtgt gagtcgatta cgaagggctt gtcgagagaa tccagaattt
     4021 tcttggcgac gagtccgctt tgctcacgct tgtggattga gaccagcgac acgacatatt
     4081 catccgcccc gaatcggctc aggatgtcac catcacgcag ggccgcctgg aaacgttggc
     4141 caacctgacg cagcaattca tcgccgactt cgtggccgag cgaatcgttg atcgaggtaa
     4201 accggttgag gtcgacgacc agcatggcgc cgtactcgct atttcggcgt gcgctgatca
     4261 aggcttgttc taccagttga tagaacagtg tgcgattggg caagccggtc agactgtcgt
     4321 aattgacaat ctgctgcatg cgttgttcgg ctgcgcgcaa gacgctgatg tcggagaaga
     4381 ttgaaaaggc atgcgtaatc acgccatcac gatcacgcgc aaccccgatg catacagatt
     4441 gcgggaacag ttctccattc tttcgcctgc cgatgatttc tccctgccat gcacccgctc
     4501 cgtgcattgc tgcgagtact tcagcccgga aatcggcact gtgcacaccg gagcgcagaa
     4561 ggtctggtgt tttaccgatg gcttctccag ccgaatatcc ggtaatgcgg gtgaacgccg
     4621 ggtttaccga gacgatccgt tctttggaat cggtaatcag aatgccttct tcactatttt
     4681 cgatgatccg tgcatgcagg cgctgctttt cagcagttga aagttgatcg gcaatttctg
     4741 aaagatgcgc caccaatccg atcgcttcgt tgttcttgtc aaggacatgt tcgagcgaaa
     4801 gattggcacg gaaggtctct ccggatttct tgcggcgtcg aacctccatc aaggggctgc
     4861 cgttgtcgag aaagaattcg tgaatttcac tgttgcggtc agtgggttcg tcggcnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaggca gtgctcaata cgcgaatcag
     5041 gcatcaacgg cgtccttctg ttcgtggatt acgccaagca tccattggta tgcctctaga
     5101 ctaagcggat tgtagtcagc aatcgacagg ccaaagccgc tcagaatgcg cacaagaacg
     5161 ggctcctcgg tacattctgt gctctggact gccagtagaa gctgccccag ctctccttca
     5221 tgtttcagga ccgcattggt cagcgaatcg cttagtctca gcggtttcag aatatcaacc
     5281 agcggtaggc caaacagggt tccaagcagc gaaaacattc cggccatgaa ggcttgctcc
     5341 tgcaccgaac gatcaagccc gccggctttg gccagcaatt ccatggttcg tgcgcggacg
     5401 gcaacccgcg ccagcaacat ggcggaacga tggtcatcac gacttgccgc aaacagcatc
     5461 aagttcagcc aacgcttgag ttgttgcctc ccgaggatga ggatcgcttg cgaaaaactg
     5521 gaaatatgcc tgccaacgcc tactccaagc gaattgacaa ggcgcaggag atgaaacgcc
     5581 agaacaggat cctgacgaaa tatggcctcg atttcacagg tatcggcatc cgtcgccaca
     5641 agctgcagca gcttgagcga cacggttcgt gaagtcgcct ggctagccga ctgtctggcc
     5701 ggaggggcgc aataccagtc accggtcagc cactcggccg tggttggaag taccggtttt
     5761 gtttgctcgt cggaattgta ggactgttcc tcgcgcatga tctgacatcc tgcgttctgc
     5821 agtgctgtca gcaactcggg gggcaagcgc tcggcaaggc tcgcatcgaa caggcaggga
     5881 aacgttgccg aaaatgtgga gaaatcttca ccctcaagca gtggcaacaa ggcgctcatg
     5941 tcacctttac caacgcgcag cattaccgcc gccgggttca gatttctatc agccagaaga
     6001 gtgaatgata gaagcggcgg caaactcatg atgatccccg tgaatgttgt ctggtggcaa
     6061 tcgcgttctg tttccgaatg cggcgacgat acttgacttg accgataacg gcacgaatta
     6121 ctaaaactca agagcaatcg acacgtgcaa atcgagtttc gatcgctggc ccggcgttcg
     6181 tgttggccgt ggataggtac agaccgcgtt tccgtagcta ttctctctca ataacatgtc
     6241 aattcggttg gcgatgtttt aaaattcagt tgtcacgcca tcgcgggatc gatatccgca
     6301 agaaggggag tgtcacggac tccgcagggc gattggccgg cggggaaact accgcatagc
     6361 cgagtcgagt tgaatatgcc tcacagagtt cttaaagtac ctgagcgcgg tatgctcgat
     6421 gcctacgagg ctgttttgtc cgagcgcggt ttctcggctg atgctgcgca acgtgccgcg
     6481 gctgagcgac ttcaactgct ctactatcag ttgctttcct tcaaagtggg tcgtaagagc
     6541 actctgcgtc gtgtgttttc tcctccggtc gtgccgcgcg gcgtatattt ctggggtggc
     6601 gtcggacgcg gcaagagttt cctgatggat tgcttctacg attcagtgcc ttacgtgcgc
     6661 aaacgacgca tccactttca cgccttcatg cacggtgttc atcagcagct gaatgtgttc
     6721 aagggcgaga gtgacccgct gacgaaagtg gctgaacgca tagccgacga gtgccgactg
     6781 atttgcttcg acgagtttca tgtctcggac atagccgatg caatgatcct gcgtcgcttg
     6841 ctcgaagcgc tgtttgagcg cggtgtggtt ttcgtcatga cttcgaacta tccaccgaac
     6901 aggctttacc cgaatggatt gcagcgggaa aacttccttc cggccatcgc cttgattgag
     6961 tcgaatgtgg atgtcgttga agttgattcc ggtgtcgatt atcggctgcg aactctggaa
     7021 caggtcgaga ttttccatta ccctgccgat gcgaatgccg aaagcaaaat ggccggatat
     7081 ttctcttcga tcgctggagg cgaaggtgct caaggaggca gcgtcgacgt gcttggacgt
     7141 gccataccaa cgctgcgcca ggggacgggt ggagtaatct ggtttgactt caagtccttg
     7201 tgtggcggtc cgcgctcaca gaatgattat cttgaactgt cccgttccta tcatacgatc
     7261 ctgctttccg gggttccgag gatgactgcc agcatgtctt ccgaggcgcg acgctttacc
     7321 tggcttgtgg atgttcttta tgaccacaag gttaaactta ttgtgacagc ggattgcgag
     7381 ccggagctgc tttatgttga aggtacgcag gcgagtgagt ttttccgaac cgtcagtcgg
     7441 ctgaccgaga tgcgttcgcg cgagtacctt gcacttccac atttgtcctg acgacccttt
     7501 ctgcgcaggg taattcggga gcacgaggag tgttacatgc acagattgtt tctgcttgcc
     7561 gggcttggac tttccgggct gtcctttgcc cagcaagtca tggagattat tcctctcaaa
     7621 tatcgcacgg tcgaacaggt cttgccgtcg cttcaggtat tgattgagcc ggggggcgcc
     7681 ttgtccggga tgaacaatca gttgattgtt cgatcatccg gcaagaatat tgcacagatc
     7741 aggcaggcgc tttccgcaat tgacttgccc gcccgtcggc tgatgatcta cgtcagtcaa
     7801 aaccgtgaca ccgaaagccg gcaacaaggc gcggaggtat cgggccaggt cggtttcggc
     7861 aacaatgttc gcattattca gccgtcgagt ggaatgcagg gaagcacacg gatcgacgtg
     7921 cggagcggtg attcagacgt aaattaccgg atcaatgcac aggctcatga ttcgagggtg
     7981 accagtgaca tggctgcaac tcagagcgtg caagtggttg atggtggcca ggcactcatc
     8041 cgggtcgggc aatccttgcc gatgcccttg cgacaggtcg tgcgtggtcc gggcggcgtg
     8101 acggtcaccg atagcgtggt ctatcgtgac attggtcaag ggttttatgc cgcaccgcgt
     8161 ttggtgggta accaggtgac gctggaaatc agcccgcaat tcgatacgcc gggcaatcag
     8221 ggttacggca gcgtcaattc gcaaagagtg tcgacgacaa tctccgggcg tttgggcgag
     8281 tggatcgaac ttggcgggag tggccagcag atggcgggta acgtgcgagg aaacctgagc
     8341 tccgcgactc aggaattgcg cgattaccga agcgtatggc tgcgtgtcga agaattgccc
     8401 tgagtcgcgg acactgaact acggctcaag caagaggata tccccgtcca ggcagaaacg
     8461 gttgcgtccc gcttcccggg cgtggatgag cagcgatttt gcgcgctcca gcaaggtttc
     8521 cgagttttct ccggtgtatt cggtgatacc gccactgacg gttatcctta ggcctgcgct
     8581 gacgaattcc tgcgtttcga tcaacatgcg caggcgctcg agtccgatca tggcttcctt
     8641 gagggaaaca ttgggcagta ccaataaaaa ctcgtcatcg ccaatgcggc ctgcgatatc
     8701 gatttcgcgc atggtttccg tgagtagttt gccgatcccg gacagcacgc gctcaccggg
     8761 ttccggacca tgtttctcgc agaagtgcag gaagtggtct atatcaacct gtgccaaggt
     8821 gaatggcgta ccgtaacggc gggcacggtg aatctcgttt tccagcaagt acatgacatg
     8881 tcgctggttg tacacgccgg tcagttggtc ccgaaccgag agcttcttga cttcgctttg
     8941 caggtccttc tctttgttga tgtctgtcag cgtgagcagt acccgccgca cgcctccgtt
     9001 gtcagaaaag acaggcaacg cattgacgct cagccagttt ttgcgttcct gatcattggt
     9061 cagcgccatc acgcagttga ataccggctt tgatgtgcgc agagcccgct ttagcggcag
     9121 ttcgtcggcg caaataatct gaccgctgtc attgacccag ttaggcggag tattcccaat
     9181 cagcttgctg gcgaccttgt tgctcaagca cggcgaggca tccgggtaaa acaccaccag
     9241 gccgataccc aggctatcga gcaatcctgc atagcgcttg atttcttcgt ccttggcgtg
     9301 cgtgttcggc agaggcgaaa agtactcggc tggattcatc ggctttgacc gggttcgtga
     9361 cgggagcaat ataccaatcg acaggcagaa aaaccaatga atgattgaag aaaccgccga
     9421 caggcttgat taatctgacg aacgggtcta cgcagccgtc agtgcctgat cgagatcggc
     9481 aatcagatcg tcgatgtgct cgatgccgac cgacaggcgg atcatgtctt ccgatacgcc
     9541 ggccttgacc agttcctcgg gtgacaactg ccgatgagtg gttgacgccg gatgacaggc
     9601 gagtgatttt gcgtcgccga tattgaccag ccgggtgaac agttgcagcg catcctggaa
     9661 gcggccaccc gcagcaaagc ctcccttgac gccgaaggtg aggatgcctg aagcaaggcc
     9721 atccatgtat ttctggacca gtgcgtgatc cttgtgatcg ggcaatccgg cgtaattgac
     9781 ccacgacacc ttggcatgac tcttgagaaa ctgcgccact ttggtcgtgt tttcacagat
     9841 ccggtccata cgcaa
//
LOCUS       DAORPM010000073         9682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1635_length_9682_cov_9.476992, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000073 DAORPM010000000
VERSION     DAORPM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1635_length_9682_cov_9.476992"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLD53_05155"
     CDS             <1..235
                     /locus_tag="PLD53_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRC60331.1"
                     /translation="EELRGLLGKLGMRPAELVRRGEEVFKTDYAGKNLSDDEWLDALV
                     AHPILIERPIVVHGDKAVIGRPTERVRELLDDD"
     gene            359..>1000
                     /locus_tag="PLD53_05160"
     CDS             359..>1000
                     /locus_tag="PLD53_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60332.1"
                     /translation="MATREGVTVPIYTFWRDDALATVVLFSGGGGGYGKIGEDGWPAG
                     GNFLIRTGKHWASHPFNVVMVGRPTDGIDLSNGAVRIGEEHAADNVAIFKAIKNRSAR
                     PIWVVGTSMGTISAAAAAIHDSERLVSGVVLTSSVVAYKIAGAVPKQDLDKIRVPTLV
                     LHHEHDACWACRPYEARNIVDGLKNAPVRKLTLVSGGSGATGDPCEPLHYHGYA"
     assembly_gap    1001..1010
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1095..1424)
                     /locus_tag="PLD53_05165"
     CDS             complement(1095..1424)
                     /locus_tag="PLD53_05165"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HRC60333.1"
                     /translation="MQMTDIPFGTTDWSKIPQTEHRGDAGLALWRTQHFSDIRVRLVE
                     YTPGYLADHWCVKGHILFCIEGELHTELDDGREFTLKPGMSYQVADNAEPHRSSTAHG
                     AKLFIVD"
     gene            1485..2186
                     /locus_tag="PLD53_05170"
     CDS             1485..2186
                     /locus_tag="PLD53_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC60334.1"
                     /translation="MNGPTYQVLVTGANRGLGLEFVRQYASAGCHVIACCRDPLKATE
                     LNQLATDSAGKIAVHALDVSEFSQIERLASELNGQAIDVLINNAGVYPRSSFGSIDYA
                     EWHKAFLINTMAPMRMVECLVDHVAASRLRKIVTLSSKMGSITDNDSGSSALYRTSKA
                     AVNMVMKNLAIELKSRGIAVGTLHPGWVKTDMGGANALINAQQSVAGMRTVIEQVSLD
                     DTGRFIAFDGQEVPW"
     gene            2320..2775
                     /locus_tag="PLD53_05175"
     CDS             2320..2775
                     /locus_tag="PLD53_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60335.1"
                     /translation="MYTVDDPTLALITRFMGDLPRPEISDAEFFHQQVAAIEEYVARF
                     PPQEQEQRAMAWIETNARRYRQKWEKLATIDVLTETRCPDCPLSGGSDKERCEIHARW
                     LTLLRRYAASELSSRDYVKTSLELLAAHKNRLKVNDQHGLLCKHPTPSS"
     gene            complement(2817..3161)
                     /locus_tag="PLD53_05180"
     CDS             complement(2817..3161)
                     /locus_tag="PLD53_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HRC60336.1"
                     /translation="MPLLKIQTNVILDAEAGKTLVADASRAVAAMLGKPERYVLVSLE
                     SAATMAFGGDSSPLAYLEMKSIGLPQGRTAEFSKALCKLMSTALGVSADRVYIEFADA
                     QGSMWGWNGGTF"
     gene            complement(3240..3944)
                     /locus_tag="PLD53_05185"
     CDS             complement(3240..3944)
                     /locus_tag="PLD53_05185"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60337.1"
                     /translation="MENISIQLEPIRAILYQIGAFMPRLLIAALVVIGGWLLAKIVLI
                     AVTKGLRAINFNILTERSGMDGFLKQGGVQSDTTDIVGILCYWLVILAALLIAFNGLG
                     LTYITDLLSRIVWFVPNVFVAILVLAFGSYFARFIGDAVATYCRNVQMQDAAFLGKVA
                     QYAIMVFVTLIALDQIQVGGDIVRQSFLVILAGIVFSLALAFGLGGKNWAAERIEQWW
                     PSDKKVADEADQSAER"
     gene            complement(3987..7328)
                     /gene="treS"
                     /locus_tag="PLD53_05190"
     CDS             complement(3987..7328)
                     /gene="treS"
                     /locus_tag="PLD53_05190"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HRC60338.1"
                     /translation="MTTLAQASVPEGSAIALSDDPLWYRDAVIYQLNVKAFYDTNDDG
                     VGDFKGVTAKLDYVKELGVNTIWLMPFYPSPLRDDGYDISEYEDVHPQYGTLDDFREM
                     MTEAHKRGIRVITELVINHTSDQHPWFQAARKAPAGSPERDFYVWSDTDELYKGTRII
                     FTDTETSNWTWDPVAKAYFWHRFFSHQPDLNFDNPAVMEAVLKTMRFWLDMGVDGFRL
                     DAIPYLIERDGTSNENLPETHAVIKKLRAAIDAEYKNRFLLAEANMWPEDVREYFGDG
                     DECHMAYHFPLMPRMYMAIAQEDRRPIVEIMQQTPDIPEGCQWAIFLRNHDELTLEMV
                     TSKERDYMYQMYAADRRARINLGIRRRLAPLMENDIDAIKLMNGMLLSMPGSPIIYYG
                     DEIGMGDNVFVGDRNGVRTPMQWSPDRNAGFSHAHPQTLYLQPIIGAVYGYEALNVEA
                     QAREASSLLNWTKRMLAVRKTSHAFGRGKRSFLKPGNGKILAYISEYEDDTLLAVFNL
                     SRAAQPVELDLSAYKGRVPVEMLGRTSFPPIQDGHYVLTLPSHSFYWFRLAADAEMPA
                     WHEESVAWTERPTLVLFDAWTSFFRDKVMPWRMGMAERMRNQFETDTLPRHIETQRWY
                     ASKGTAIARARIADYAIWNQGDFSWILPITQLDGPEEESSYFVPLALAWEDHDEDRLH
                     RLSGAAVARIRQQANVGLMGDAFFDESFCRALVQAIGSNLDLATANGKLHFKATAAYA
                     ALSVDVESLAVSRPSAMSSNTVVTLDETLFLKGYRRLREGMNPELELGRFLTDVVSYR
                     NCVPVLGALEYFGNDGSVMTLALVQSHVSNQGDGWAFTVSYLERHLDDFRLAIGAAVD
                     ATESDEAHGGFLTLAATLGLRTAELHLALATRTGNPAFDPEPLLASDVVAFRQRAALE
                     ADSTLELLGQRLEQLPETVQGDARAILDQRDLLLQRINGNGTDVDGGIKTRYHGDYHL
                     GQVLVTDNDFVIIDFEGEPARSFEERRTKSSPLRDVSGMLRSFNYARWSALRNISQGT
                     EELERVLVAARDWELATRKAFMDAYMGTMGSSATATPVNADLLALFELEKALYELRYE
                     INNRHDWAQVPIQGILALLDPDTAR"
     gene            7577..8446
                     /locus_tag="PLD53_05195"
     CDS             7577..8446
                     /locus_tag="PLD53_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRC60339.1"
                     /translation="MRLDDQNESDNVEDRRGTGGNFGGGVRRGGVGIGAIVLALAASY
                     FFGIDPSVILGLASNIDSQPSQHTAARKPPAADEMARFVSKVLASTESTWDEVFREGG
                     SRYQEPKLVLFSGATPTACGTGQSAMGPFYCPSDQKVYIDLSFYRDLKQRFHAPGEFA
                     QAYVIAHEVGHHVQNLLGISDKVHRAQQGASKSRANALSVRLELQADCFAGVWGHRAD
                     AAKNIIEPGEIEQALNAASAIGDDRLQQQTQGRIVPESFTHGSSEQRMRWFKRGIESG
                     DLRQCDTFKAASL"
     gene            8464..9312
                     /locus_tag="PLD53_05200"
     CDS             8464..9312
                     /locus_tag="PLD53_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC60340.1"
                     /translation="MTPSLMRLVLVLAILTQSSVAGALPKAESVPGGIALVDLGVVKS
                     RGNPPRAWFGEQPVLVAAEKGRWVAVLGLALDLPAGTHRLRAIVAGEEKTLPFEVGEK
                     NYPEQRITLKDSSKVNLSAADEARAVSEIAEIQGFKRHWREADDTDSDFVLPAEGRLA
                     SRFGLRRFFNGEPRSPHSGLDLAVPRGTPIKASAHGQVLAVDDYFFNGKTVFVDHGNG
                     LISMYCHLDRIDVQAGDSVGRGQRIGLSGKTGRVSGPHLHWSVILNGAMVDPALFVAA
                     SKKSTY"
     gene            complement(9309..>9682)
                     /locus_tag="PLD53_05205"
     CDS             complement(9309..>9682)
                     /locus_tag="PLD53_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRC60341.1"
                     /translation="IDSTGLLVLTQNGRVARQLVGENSTVEKEYLVRVEGRLDAEGLA
                     LLNHGLSLDGRRLKPASVEWINDDQLRFVLNEGRKRQIRRMCELVGLRAVGLKRVRIG
                     KVKLGDLPLGQWRFLRDDETF"
BASE COUNT         1981 a         2868 c         2914 g         1909 t
ORIGIN      
        1 ggaagaattg cgcggcctgc tgggcaagct cggcatgcgt cctgctgaac tcgtccgtcg
       61 tggcgaggaa gtcttcaaga cggattatgc gggaaagaac ctgagcgacg acgagtggct
      121 ggacgcgctc gtcgcccatc cgattctgat cgaacggcct atcgtcgtgc acggcgacaa
      181 ggcagtgatc ggacgcccga ctgaaagggt gcgggaactg ctggacgacg actgaaaggc
      241 tgccatggcg ttttcgcgag aactcttgag aatgtggcgt gcagtcgtgc ttgcggcgac
      301 gatgctcgct ggcggcgccc tctacgcgca cgcagcgggc gtcggtgagg ttctgtgagt
      361 ggccacgcgc gagggcgtta ccgtgccgat ctacacgttc tggcgcgacg acgcgctggc
      421 cacggtcgtg ctcttttcgg gcggcggtgg gggatacgga aagatcggcg aagacggctg
      481 gccggcagga ggcaacttct tgattcgcac cggcaagcat tgggccagcc acccgttcaa
      541 tgtcgtcatg gtcggtcgcc cgacggacgg catcgacctt tccaacggcg cggtgcgcat
      601 tggcgaggag catgctgccg acaacgtggc gattttcaag gccatcaaga accggagcgc
      661 gcggccgatc tgggtcgtcg gcaccagcat gggaacgata tctgccgcgg cggcggcgat
      721 tcatgacagc gagcgtcttg tttcgggcgt ggtattgacg tcgtcagtcg tcgcctacaa
      781 gattgccggt gcggtgccca agcaggatct cgataagatc cgcgttccga ctttggtttt
      841 gcaccacgag catgacgcct gctgggcttg ccggccatat gaggcaagaa acatcgtcga
      901 cggcctgaag aacgcaccgg tcaggaagct aaccctggtc agcggcggtt caggcgcaac
      961 gggcgacccc tgcgagccct tgcactacca cggctacgcc nnnnnnnnnn ctacgccggt
     1021 atgcaaaatg aggccgtcga tctcatcgcc gcgtggatcg tcaacccgac ggaataaatg
     1081 cctggccggc agactcaatc gacgatgaac agtttggcac catgtgccgt gcttgagcgg
     1141 tgcggctctg cgttgtcggc gacctggtag ctcatccccg gcttgagcgt gaactcgcgc
     1201 ccatcgtcga gttccgtgtg cagctcgcct tcgatgcaga acaagatatg ccccttaacg
     1261 caccagtggt cggcgagata gcctggcgtg tactccacca ggcggacccg gatatcacta
     1321 aaatgctgtg tacgccacaa agccagaccg gcgtctccac ggtgctccgt ttggggaatt
     1381 ttcgaccagt cggtagtgcc gaatgggatg tctgtcattt gcatggcaat tcctttgcga
     1441 taggtcgtaa tgagcacgaa taacgtaatt gaaaggcaag cccaatgaat ggtccaacat
     1501 atcaggtact cgtcaccggc gccaatcgtg gccttggcct ggagtttgtc agacagtacg
     1561 ccagcgccgg ttgccacgtc atcgcctgtt gccgcgatcc gctaaaggcg acagagctca
     1621 atcagctggc gacggatagt gcaggaaaga ttgcggtgca cgccctcgat gtctctgagt
     1681 tcagccagat cgagcgtctg gcatcagaat tgaacggcca ggccatagac gtactcatca
     1741 acaacgcagg agtctatcca cggagttcct ttggctcgat cgactatgcc gaatggcaca
     1801 aggcgttctt gatcaacacc atggctccga tgagaatggt cgagtgtctt gtcgatcatg
     1861 tcgccgccag tcggctgcgc aagatcgtga cgctctcaag caaaatgggc agtatcacag
     1921 acaatgacag cggcagcagt gccctctacc gaaccagcaa ggccgccgta aacatggtga
     1981 tgaagaatct tgccatcgaa ctgaagtctc gcggcatcgc tgtcggcacg cttcatcccg
     2041 gatgggtcaa gaccgatatg ggcggtgcca atgcgctgat taacgcgcag caaagcgtcg
     2101 ccggaatgcg gacggtgatc gagcaagtat cgctcgacga caccggccgg ttcatcgcgt
     2161 tcgacggcca ggaggtaccc tggtagattc acgacggcca cgcacagtcc cgtacaccac
     2221 cacccgcctg tgaagcgaaa aataaccttg tgcaccgcag caatctttgc tatatttccg
     2281 tcatctgatt gagttctctc tctgctcgac gggaagcgta tgtacaccgt ggatgacccc
     2341 acccttgccc tcattacccg tttcatgggg gacttgccgc gtcctgagat atcggatgcg
     2401 gagttctttc accagcaggt cgcggcaatc gaggagtacg tcgcgcgatt cccccctcag
     2461 gagcaggagc agcgggcaat ggcatggatt gaaaccaacg ccagaagata tcgccagaag
     2521 tgggaaaaac ttgcgacaat cgatgtcctg acagagacgc ggtgtcctga ttgccccctg
     2581 tcaggtggga gcgacaagga acgctgcgaa atccatgcac gctggcttac gctcttgagg
     2641 cgctatgccg ccagcgaact ttcttcacgc gactacgtga agacaagcct ggaattgctg
     2701 gcggcgcata aaaaccgact gaaggtgaat gaccagcatg gtctgctgtg caaacaccca
     2761 acgccgtcta gctaagctgg cggaaccaat ccccccggca gacgcacccg atgtgatcag
     2821 aacgtcccgc cgttccatcc ccacatcgag ccttgggcgt cggcgaactc gatatagacc
     2881 cggtcagcgg aaacgcctag cgcggtgctc atgagcttgc acaatgcctt ggagaattcg
     2941 gcggttcgcc cctgcggcaa gccgatgctt ttcatctcca gataggcgag cggcgaggag
     3001 tcgcccccga aagccatggt cgcggcggac tccagcgaca ccaggacata acgctcaggc
     3061 tttccgagca tcgctgcaac ggcacgcgac gcgtcggcta cgagtgtctt tccggcttcg
     3121 gcgtcgagaa tcacattggt ttgtatcttc agcaaaggca ttggggttct ccagtttgcg
     3181 tcgctgtgtt ggcagtcagg aattccagcc gcacgatcct gagttttcaa atcaaggtgt
     3241 caacgctctg cgctctggtc tgcctcgtcc gccaccttct tgtcgctcgg ccaccactgt
     3301 tcaatccgct ccgccgccca gttctttccg cccagaccaa aagcgagggc cagcgaaaat
     3361 acaatgccgg ccaggatgac cagaaaactc tggcgaacga tatcgccacc gacctgtatc
     3421 tggtcgagcg cgatcagggt cacgaaaacc atgatcgcgt actgagccac cttgcccaga
     3481 aacgctgcgt cctgcatctg gacattgcgg cagtacgtgg caacggcatc gccgatgaat
     3541 cgtgcgaaat acgacccgaa tgccagcacc aggatggcga caaacacgtt gggcacgaac
     3601 cacacaatcc tgctgagcag gtcggtgatg taggtcagcc ccaggccgtt gaacgcgatc
     3661 agcagagccg cgaggataac cagccagtag caaaggatgc caacgatgtc ggtcgtgtcg
     3721 gactgtaccc ccccttgttt gaggaagccg tccatgcctg aacgctcggt caggatgttg
     3781 aagttgatgg ctcgcagacc tttggtcacc gcgatcaaga caatcttggc cagcagccag
     3841 ccgccgatga caaccagcgc tgcgatcagc aggcgcggca tgaacgcgcc gatctggtaa
     3901 aggatggctc ggatcggctc cagttgaata ctgatgtttt ccatgatgtg taactccagg
     3961 tagggaaagc gggaccagtg caggcgtcag cgtgcagtgt cagggtcgag cagcgccaga
     4021 atgccctgga tcggcacctg ggcccagtcg tgacgattgt tgatctcgta gcgcaattcg
     4081 tagagcgcct tctccagttc gaacaaggca agcaggtcag cattgaccgg ggtcgccgtt
     4141 gctgaggacc ccatggtgcc catataggcg tccatgaatg ccttgcgggt cgccagctcc
     4201 cagtcacgcg cggcgacgag aacccgctcc agttcctccg tgccctggga gatattgcgc
     4261 aacgcggacc agcgcgcata gttgaaggaa cgcaacattc cggacacgtc gcgcagcggt
     4321 gaactcttgg tacggcgttc ctcaaagctg cgcgccggct cgccttcgaa atcgatgatc
     4381 acgaaatcgt tgtcggtcac cagaacctga cccagatggt agtcaccgtg gtaacgggtc
     4441 ttgataccgc catcgacatc ggtaccgttt ccgttgatac gctgcagcag caggtcacgc
     4501 tgatccagga ttgcgcgtgc atcgccctga actgtttcgg gcaactgctc cagacgctgg
     4561 ccaagcaatt ccagcgtgct gtcggcctca agagccgcac gctgccggaa tgcaaccaca
     4621 tcgctcgcca gcagaggttc gggatcaaag gccggattac cggtcctcgt ggccagggcg
     4681 aggtgcagct cggcggtacg tagccccagc gtcgccgcca aggtcaggaa gcccccgtgt
     4741 gcttcatccg actctgtcgc atcaacggct gccccaatgg ccaggcggaa atcgtcaaga
     4801 tgtcgttcaa gataactgac ggtaaaggcc cagccgtcgc cctgattcga gacatgtgac
     4861 tgaacaagcg ccagggtcat gacgctgccg tcattgccga aatactccag cgcaccgagt
     4921 accggcacac aattgcggta agacaccacg tcggtcagga aacggcccag ctcgagctcg
     4981 gggttcatgc cctcgcgcaa acgccggtaa cccttcagga acagggtctc gtcgagagtt
     5041 acaacggtat tgctgctcat cgcgctcggg cgcgagaccg ccagcgattc gacgtcgacc
     5101 gacaaggccg cataagccgc cgtggccttg aagtgcagtt tcccgttggc ggtagcgagg
     5161 tccaggttgc tgccgattgc ctgcaccaaa gcacgacaaa atgactcatc gaaaaaggcg
     5221 tcacccatga ggccgacgtt ggcctgttgg cgtatcctgg ccaccgcagc gccggagagt
     5281 ctgtgcaggc gatcctcgtc atggtcttcc caggccaggg ccagcggcac gaaatagctc
     5341 gattcttcct cggggccgtc aagttgcgta atcggcaata tccaactgaa gtcaccctga
     5401 ttccagatcg cataatcggc aattcgggcg cgcgcgatgg ccgtcccctt ggacgcatac
     5461 cagcgctgtg tttcaatatg gcgcggcagg gtgtcggttt cgaactggtt gcgcatgcgc
     5521 tcggccatgc ccatccgcca gggcatgacc ttgtcgcgga agaagctggt ccaggcgtcg
     5581 aaaagaacca gtgtcggacg ttcggtccac gcgacacttt cctcatgcca cgcgggcatc
     5641 tctgcatcgg ccgccagacg gaaccagtag aagctatgtg aaggcagcgt caggacatag
     5701 tgcccgtcct gaatcggcgg gaacgaggta cggcccagca tctcgacagg aacccgccct
     5761 ttgtatgccg acagatcaag ttccaccggt tgcgcggcac gcgacagatt gaacaccgcc
     5821 agcagggtat cgtcttcgta ttcgctgatg taggcgagta tcttgccgtt gccgggcttg
     5881 agaaagctgc gtttgccgcg gccgaaggca tggctggtct tgcgcaccgc cagcatgcgt
     5941 tttgtccagt tcagcagcga gctggcctca cgcgcctggg cttcgacatt gagcgcctcg
     6001 tagccatata cggcgccgat tatcggttga agatagagag tttgcgggtg ggcgtgggag
     6061 aaaccggcat tgcggtccgg gctccattgc atgggcgtgc gcacaccgtt gcggtcgccg
     6121 acgaagacgt tgtcacccat gccgatttca tcaccgtagt agatgatcgg cgagcccggc
     6181 attgacagca gcatgccgtt catcagcttg atggcgtcga tatcgttctc catcagcggt
     6241 gcaagacgtc tgcgaatgcc cagattgatg cgcgcgcgcc ggtcggcggc gtacatctgg
     6301 tacatgtaat cgcgttcctt gctggtgacc atctccagcg tcagctcgtc atggttgcgc
     6361 aggaagatcg cccattggca cccctcgggg atatccggcg tctgctgcat gatctcgaca
     6421 atcggccgac ggtcttcctg cgcgatcgcc atgtacatgc gcggcatcag cggaaagtgg
     6481 taggccatgt ggcactcgtc gccgtcgccg aaatactcgc ggacatcctc gggccacatg
     6541 ttggcctcgg ccagcaggaa gcggttcttg tattcggcat cgattgccgc gcgcagcttc
     6601 ttgatcacgg catgggtttc cggcaggttt tcattgctgg tgccgtcgcg ttcgatcagg
     6661 tagggaatgg cgtccagcct gaagccgtca acgcccatgt caagccagaa acgcatggtt
     6721 tttagtaccg cctccatgac cgccgggttg tcaaaattca gatcgggctg gtggctgaaa
     6781 aagcggtgcc agaaataggc tttggcgacc ggatcccagg tccagttcga cgtctcggtg
     6841 tccgtgaaaa tgatgcgggt acccttgtag agctcgtcgg tatcgctcca gacatagaag
     6901 tcgcgctcgg gcgaacctgc cggcgccttg cgcgccgcct ggaaccacgg atgctggtcc
     6961 gatgtgtggt tgatgaccag ttcggtgatc acacgtattc cgcgcttgtg ggcctctgtc
     7021 atcatttcac ggaaatcgtc gagcgtgccg tactgcggat ggacgtcctc gtactccgaa
     7081 atatcgtagc cgtcgtcgcg caatggcgac ggatagaacg gcatcagcca gatcgtgttg
     7141 acgcccagct ctttgacata atcgagcttg gccgtgacgc ccttgaaatc ccccacaccg
     7201 tcatcgttgg tgtcgtagaa agccttgacg ttaagctggt agatgacggc gtcgcggtac
     7261 cacagcgggt cgtcgctgag agcgatggca gaaccttcgg ggactgaggc ttgggcaagt
     7321 gtagtcattg gtgggggcaa tcgaaaaata tatctggaat cgcgtccatc gcgaatgagt
     7381 cggcgcacgc tagtcaactc tgcgcagcta tgccggcaag ctgtgaggat cgctgcgact
     7441 cggcatcggg aaatccagcg caacgtgcgt tagtttctct gacacaggct gaccttgtca
     7501 attgtcgaat agttccctca agtcaaaacg ctgctgttaa catcttggct ggtgcgccac
     7561 ggaaacggat tgaatcatgc gacttgatga ccagaatgaa agcgacaatg tcgaagaccg
     7621 aaggggcact ggcggcaact tcggcggcgg cgtcaggcgc ggtggcgtgg gcatcggcgc
     7681 catcgtcctg gcgctggctg ccagctattt tttcggcatc gatccgtcgg tcatcctcgg
     7741 cctcgcttcc aatatcgaca gccagccttc tcagcatacg gctgcgcgca agccgcccgc
     7801 cgccgacgaa atggcgcgct ttgtctccaa ggttttggcc agtaccgaga gcacctggga
     7861 cgaggtcttc cgcgaagggg gctcgcgtta ccaggagccc aagctggtgc ttttcagcgg
     7921 cgcgacgccg acggcctgcg gtaccggcca gtctgcgatg ggtccgttct actgcccgtc
     7981 cgatcaaaag gtatacatcg atctttcttt ttaccgcgac ctgaagcagc gcttccacgc
     8041 gccgggcgag tttgcccagg cctatgtgat cgcccatgaa gtcggacacc atgtgcaaaa
     8101 cctgctcggt atttccgaca aggtgcatcg cgcacagcag ggcgccagca agtccagggc
     8161 caacgcgctg tcggtacggc ttgaattgca ggccgactgc tttgccggcg tctgggggca
     8221 ccgtgccgat gcggcaaaga acatcatcga gcccggtgaa atcgagcagg cgctgaatgc
     8281 cgcttcggcc atcggcgatg atcgcttgca acagcagacg caaggccgga tcgttccgga
     8341 atccttcacc catggcagct cagaacagcg catgcgctgg ttcaagcgcg gcatcgagtc
     8401 aggcgatttg cgccaatgcg acactttcaa agcggcgtcg ctgtaggcca tgaaggcgca
     8461 tagatgacgc catccctgat gcggctcgtg ctggttctgg cgatactgac gcaatcgtca
     8521 gttgccggcg ccctgcccaa agcggaaagc gttcccggcg gtatcgcgct ggtcgatctg
     8581 ggcgtagtca aaagtcgcgg caacccgccc cgtgcctggt ttggcgaaca accggtgctg
     8641 gttgccgccg aaaagggccg ctgggtagcg gtgctcggtt tggcgctgga tctgccggcg
     8701 ggcacccatc gactgcgcgc cattgtcgcg ggcgaagaga agactctgcc attcgaagtc
     8761 ggtgaaaaga actacccgga gcaacgcatc acactcaagg attcgagcaa ggtcaatctt
     8821 tctgcggccg acgaggcgcg cgccgtcagc gaaatcgcgg agatccaggg tttcaagcgg
     8881 cactggcgcg aggctgacga taccgacagc gatttcgtgc tgcctgccga gggacggctg
     8941 gccagtcgtt tcggactgcg ccgcttcttc aatggcgaac cgcgctcacc gcattccggg
     9001 cttgacctgg cagtaccgcg cggcacaccg atcaaggcca gcgcacacgg ccaggtactg
     9061 gcggtcgatg attatttttt caacgggaaa acggtgtttg tcgatcacgg taacgggctg
     9121 atcagcatgt actgtcacct cgaccgcatc gacgttcagg ccggcgattc agtcggcagg
     9181 ggtcagcgaa tcggcctctc cggaaagacc gggcgggtca gcggcccgca cctgcactgg
     9241 agcgtgatcc tcaatggtgc gatggtcgat ccggcgttgt ttgttgcggc aagcaagaaa
     9301 tccacgtatt agaacgtttc gtcgtcgcgc aggaaacgcc actgcccgag cggcagatcg
     9361 cccagtttca ccttgccgat gcgcacccgt ttaagcccga cggcacgcag gccgaccagc
     9421 tcgcacatgc gtcgaatctg ccgtttgcgc ccttcgttca gcacaaagcg aagctgatcg
     9481 tcgttgatcc attcgacgct ggccggcttg agccgcctgc cgtcgagact gaggccatga
     9541 ttcagcagtg ccagaccctc tgcgtcgagc cggccttcaa cacgcaccag atattctttt
     9601 tcgacggtcg agttttcgcc gaccaactgg cgcgccacgc gaccgttctg cgtcaacacc
     9661 agcaggccgg tcgaatcgat at
//
LOCUS       DAORPM010000074         9510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1678_length_9510_cov_9.097620, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000074 DAORPM010000000
VERSION     DAORPM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1678_length_9510_cov_9.097620"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="bioC"
                     /locus_tag="PLD53_05210"
     CDS             complement(<1..782)
                     /gene="bioC"
                     /locus_tag="PLD53_05210"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HRC60342.1"
                     /translation="MRAPAQFVDLRQVRRNFARAATSYDAVAVLQREVGSRMLERLDY
                     VKIEPRRVLDVGCGTGISLTALSERYPKSKVIGADASEAMLRTGRTQRSGLRWLLPFL
                     RSSRSSLISADALALPFTAGSLGLVWSNLMLHWLDDPLPAFHEIYRALDVGGLLMFST
                     FGPDTLKELRTSFSDGYAHTQRFADMHDYGDMLLECGYSDPVMDVEALTMTYASLDDL
                     FRDLRQSGAACAMQARRRGLMGRSAWNAVRTGYERHRSEGRLP"
     gene            complement(789..1778)
                     /gene="bioB"
                     /locus_tag="PLD53_05215"
     CDS             complement(789..1778)
                     /gene="bioB"
                     /locus_tag="PLD53_05215"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRC60343.1"
                     /translation="MNTISPAVALTPDTSAPVRSAKWTVSQVEALYQMPLMDLLFRAQ
                     QVHRENFDPNEVQRSTLISIKTGGCSEDCSYCSQSARYKTETEREALMQVEDVLAAAR
                     EAKAKGASRFCMGAAWRGPKEKELEVVTQMIREVKSLGLETCVTLGMLKEGQADQLKE
                     AGLDYYNHNLDTDAEFYGQVITTHKHADRVDTISQVRHAGIKVCSGGILGMGESRRNR
                     AAMVAQLANLNPPPESVPINNLVSIPGTPLAKTEQIDNFEFVRTIAAARITMPTSFVR
                     LSAGREAMGDEMQALCFFAGANSIFYCDKLLTTSNPGIDRDQALFDKLGLRPI"
     gene            1858..2592
                     /locus_tag="PLD53_05220"
     CDS             1858..2592
                     /locus_tag="PLD53_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRC60344.1"
                     /translation="MSILTQNRGLKARLISHLRYELGQVLEQDCLLCTASNARDILCE
                     ACASELARLEEPRCPRCAIPTEHGEVCGRCLTSPPHFDATLAAYRYDFPLDKLVQSFK
                     YGQRLALGVYFGQQLAALGKRIVADLIIPLPLHPERLRARGFNQALEIARPVGKALSL
                     PIDATSCRRMRNTPAQTRLAWRDRAKNVRGAFHCSSDFTGRRVILVDDVMTTGASLDE
                     CARTLKLHGAAEITLLVVARALPKPL"
     gene            complement(2614..3762)
                     /locus_tag="PLD53_05225"
     CDS             complement(2614..3762)
                     /locus_tag="PLD53_05225"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC60345.1"
                     /translation="MSLINQVLQDLDARRATYGTGTNLPNDVRSLPKPGTPLLPIVLG
                     AAGLILLIAIVGGYAFLRQEAAPVAQLAEPVVAASSRVSPTAPPTPAPPESATSSAEP
                     PSSVTPLLLSMDGSLRLADSTDLPSAENVETKAVTATAPASGNSAAAGMKDTATEQSR
                     TKAVAPQSLSPDAAVPKAAGKSEKKPMIERTDAVGSPRERAESEYRKAIAAVNQGRVA
                     EALEELRNALRQDGLHVASRQLLVRLLLEARQPDEAAKVLQDGLQGQPAQIGWAMSLA
                     RLQVDRGDLAGAWQTLDYSSPAAGNNADYQGFMAHVLQRLGRNRDAAKHYQAATRLSP
                     NDGRWWLGLGLALEAEGQSGEAREMFLRARQSGNLGPQLTALIEQKLR"
     gene            complement(3782..4615)
                     /locus_tag="PLD53_05230"
     CDS             complement(3782..4615)
                     /locus_tag="PLD53_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC60346.1"
                     /translation="MYLKHFGLTELPFGITPDTSFIYSVQYHQEALNTLLLALSEGEG
                     FVKISGEVGTGKTLLCRRLLQTLDESWVTAYLPNPNLNADTLFLALAEELGIKDAAGL
                     DQYHLVRRINHALLGFARGKKRVVVCIDEAQVMPVETLEALRLLSNLETEKRKLLQIV
                     LFGQPELDVKLHRPEVRQLLQRIAFHYRLGGLKKSEIANYVAHRLRVAGYRGEGLFSP
                     AALRALHRASGGTPRLINILAHKSLLSVFGEGRQMAKMRHVRLARKDTDGAHRTGWLW
                     G"
     gene            complement(4641..6389)
                     /locus_tag="PLD53_05235"
     CDS             complement(4641..6389)
                     /locus_tag="PLD53_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRC60347.1"
                     /translation="MKFKLIFPLAAVVLVVGCATHQKTPGTTFDLIGTEMQGAVSSKP
                     RGADEALNQAMLPPIQLELPASAKSVEPRFDLAVSNAPATQVFMALVSGTRYSMLVAP
                     EVAGNVTVNLKSVTVREALETLRELYGYEFKFQGTRIYIQPNTLQTRIFQLNYLAGKR
                     KGQSEVSVTSNTMTAIQPQAGGATVTNTALPVSPAGNTAGPPSSQVSTSQHNDFWRDL
                     TTALTSIVGSDNGRAVIVNPGSGVVLVKAFPSDIRSVEQYLRITQLIIERQVMLEAKI
                     IEVELNEEFQAGVNWSQFGGTNNRFAYGAAQPGTVLNTTNRAGFDSGDLAQSGAGAIL
                     APGTGVLPGVGGLVAATKLGSGFFGLAFQSANFAALLNFLETQGNVQVLSSPRIASIN
                     NQMAVLKVGVDEFFVTNVSSTVTTSGTGNVTTPSITLQPFFSGIALDVTPQIDEDDNI
                     VLHVHPSISLVKAKQTVIDLGALGEFTLPLANSAVNETDSVVRVQDGNIVAIGGLMKQ
                     EQASDSNGLPGTTGSSGWGLLFGTRNSHLRKRELVILIKPTIIRNASSWKDDLVETQG
                     RIQKLDPRLARPQPGQ"
     gene            complement(6386..6784)
                     /locus_tag="PLD53_05240"
     CDS             complement(6386..6784)
                     /locus_tag="PLD53_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60348.1"
                     /translation="MADRLNSVIRALFILTLCTLMVSVRAQALLSDPTRPPAGISSPD
                     ADAGGVTGPVLQSVMIPKKGKPSAVIGGQQVKLGELYGDSRLIKLSEREAVLEGPGGI
                     ERLMLTPGIEKTNIIRKSSAAKRAQGGGKP"
     gene            complement(6769..7458)
                     /gene="gspM"
                     /locus_tag="PLD53_05245"
     CDS             complement(6769..7458)
                     /gene="gspM"
                     /locus_tag="PLD53_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HRC60349.1"
                     /translation="MKASWLALAARYDALQLRERWLVAAAVLGGIFLIGYSVFVDPAM
                     KRAQLAERSELEQRVQLANMQQQMAVLLSPSQDPDVAARIELDRLKKQLGALADRLVA
                     VEGSLVPPQRVPALLEDMIGRKAGLRLLSLKNLPVSPLLEKKSDAEQTGAAKAADKLA
                     ASSAGLFKHGVEIRLEGSYQELAAYLERLEKSQSKLLWSSVSLSAEQHPRLVLVLTVY
                     TLSLDRTWLIV"
     gene            complement(7455..8171)
                     /locus_tag="PLD53_05250"
     CDS             complement(7455..8171)
                     /locus_tag="PLD53_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60350.1"
                     /translation="MSQQINLYEARLRPRHELPVARNLGICTAALLVLMTALSLWAHF
                     DAKRKSAVAAVSQQQLADGQEKLAELTRTMSERRVSPALAAELENAKAMLVTREEVMA
                     VLDSGSLGNISGFSAMMAGFARLSQSDLWLTGFLVTVGGEEIEIRGRMLDPSKLPAYV
                     QRLSSEPAFQGRRFAALEMRGIDPEDQNNQRGAAVASESPSDGGQQSGVPRLPRFVEF
                     ALRSENAGGADNAAHQGGKR"
     gene            complement(8168..9121)
                     /locus_tag="PLD53_05255"
     CDS             complement(8168..9121)
                     /locus_tag="PLD53_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60351.1"
                     /translation="MIRFFSTPRKPGWLSILPQSGQVTLAHVVRDRNSRPEVRLLDSF
                     ALEGSERDALQRLRVARQLKSYACTTLMGSGEYNLTQLESPPVPAEERREALRWALKE
                     TVSYPLDSACIDVLDIPILGMPPGRSAGVLVVSAAEQAVRSRVGAFEEAKIILDAVDI
                     PELAQRNVAALLEDENRGLVFLRIDENGMMLTLSFHGELIAVRSGEISTLQLDGGDAD
                     QRSRTKERLVLELQRSLDNFDRQYSHIPISKVVLATYPSVENLAADLGENTYIPVKEM
                     DFSSVIDFPAIPELRDTQCQAKNLLAIGAALRTSSAAETAA"
BASE COUNT         1967 a         2959 c         2634 g         1950 t
ORIGIN      
        1 ggcaggcgtc cttcactcct gtgcctttcg tacccagtgc gtacggcatt ccaggcagaa
       61 cgacccatca gcccgcgacg tcgggcttgc atggcacagg cggcgccgct ttggcgcagg
      121 tcgcgaaaca ggtcgtccag actggcgtag gtcatggtca gcgcctccac atccatcacc
      181 ggatctgagt aaccgcattc gagcaacata tcgccgtaat cgtgcatgtc ggcaaaacgc
      241 tgggtatgcg catagccatc ggaaaaactg gtgcgcaatt ccttcaaggt atccggtcca
      301 aaagtggaaa acatcaacag cccgccaaca tcaagagcgc gatatatctc gtgaaaagcc
      361 ggcagtggat cgtccagcca gtgcagcatc aggttcgacc agaccagacc cagcgaaccc
      421 gctgtgaagg gcaaggccag cgcatcggcc gaaatcagtg aactcctgct gctgcgcaag
      481 aagggtagaa gccagcgcaa gcctgagcgc tgtgttcgcc cggttcgcaa catggcctca
      541 ctggcgtcag caccgattac tttggacttc gggtagcgct cactcagtgc cgtcaggctg
      601 atgccggttc cacaacccac atccagaacg cgccgaggct cgatcttcac atagtcgagc
      661 cgttcgagca tgcggctacc gacctcgcgc tgcaatacgg ctaccgcatc gtagcttgtc
      721 gcagcgcgcg cgaaattgcg tcgcacctga cgcaaatcaa cgaactgcgc cggtgccctc
      781 atgaactatc agatcggacg caaaccgagt ttgtcgaata aagcctgatc gcgatcaatc
      841 cccgggttgc tcgtcgtcaa caacttgtcg cagtagaaga tcgaattggc tccggcaaag
      901 aaacacagcg cctgcatctc gtcccccatt gcctcgcggc cggcggaaag ccggacaaag
      961 ctggtaggca tggtgatgcg tgcggcggca atggtgcgca cgaactcgaa attgtcgatt
     1021 tgctccgtct tggccagcgg tgttcctgga atagagacca gattgttgat cggcacggat
     1081 tcgggaggag gattcaggtt ggccagttga gccaccatcg ccgcgcgatt cctgcgcgat
     1141 tcgcccatgc caagaatacc gccagaacat accttgatgc cggcatggcg cacctgagat
     1201 atcgtatcga cgcgatcggc gtgcttgtgc gtagtgatca cctgaccgta gaactcggca
     1261 tcggtgtcca gattgtgatt gtaataatcg agcccggctt ccttgagttg atcggcctgc
     1321 ccctccttca acataccgag agtgacgcag gtttccaggc cgagtgactt gacctcgcga
     1381 atcatctgcg tcactacctc aagttctttt tccttcgggc cgcgccaggc tgctcccatg
     1441 cagaagcgcg atgcaccctt ggctttggct tcccgggcgg ccgcaagaac atcttcaacc
     1501 tgcatcaagg cttcacgctc ggtttcggtc ttgtaacggg cagactggga acagtagctg
     1561 caatcctccg aacaaccacc cgtcttgatc gagatcagtg tcgaacgttg cacttcgttg
     1621 ggatcaaagt tctcgcggtg cacctgctga gcgcgaaaaa gcagatccat caaaggcatc
     1681 tggtaaagcg cctcgacttg cgagacagtc cactttgcgg aacggaccgg agcagacgta
     1741 tcgggagtca gcgcaacggc tggagaaatt gtattcatga gcaaagccta ccggaaaaaa
     1801 tggatacttg cctggagtat gcaggcagaa tgagaaccgc tgaaggacag ttctgaaatg
     1861 tcaatactaa cccaaaacag aggattgaag gcccgtctga tcagccacct acggtatgag
     1921 ctgggccaag tgctggaaca agactgcctg ttgtgcaccg cgtccaatgc acgcgacatc
     1981 ctttgcgaag cctgcgcgtc cgaattggcg cggctggaag aaccgcgctg cccccgatgc
     2041 gccataccta cggaacacgg cgaggtctgt ggccgctgtc tgaccagtcc accacacttc
     2101 gacgcgacgc ttgccgccta tcgctacgac ttcccgctcg acaaactggt tcaatccttc
     2161 aagtatgggc agcgtttggc gcttggcgtc tacttcggtc aacagctggc cgctctgggc
     2221 aagcgcattg ttgccgacct gatcattccc ttgcctctcc atcccgagcg cctgcgagcg
     2281 cgaggcttca atcaggcact ggagatagca cgcccggtcg gcaaggcgtt gagcctgcca
     2341 atcgacgcca ctagctgccg ccgcatgcgc aatacgcccg cacaaaccag gcttgcctgg
     2401 cgtgacaggg cgaagaacgt ccgcggcgcg ttccactgct cgtcggattt caccgggaga
     2461 cgggtgatac ttgtggacga tgtcatgaca accggcgctt cgcttgacga gtgcgcccgc
     2521 acactcaagt tgcacggcgc ggcagagatc actttactgg tcgttgcccg ggcgttgccc
     2581 aagcctttgt agtactgaat tgcagcctgt ttgctagcgc aacttctgct cgatcagcgc
     2641 cgtcaattgg ggaccgagat taccgctctg tctggcgcgg aggaacattt ccctggcttc
     2701 tcccgattga ccttcggcct ccagcgctag gcccagcccc aaccaccagc gaccgtcatt
     2761 gggcgacagc cgggttgccg cctgatagtg tttggctgca tccctgtttc gccccagacg
     2821 ctgaaggaca tgcgccatga agccctgata atcggcattg ttgcctgcgg ccggtgacga
     2881 ataatcgagc gtctgccagg cacccgcaag gtccccgcga tcaacctgta aacgcgccag
     2941 gctcatcgcc caaccgatct gcgccggttg cccctggaga ccgtcctgca gcaccttggc
     3001 cgcttcatcc ggttgcctgg cttcgagcag caatctgacc agcaactgcc gggatgccac
     3061 atgcaaacca tcctggcgca gcgcgttgcg caattcttcg agtgcttcag ccactcggcc
     3121 ctggttgacc gccgcaatgg ccttacgata ttcgctttca gcacgctcac ggggtgaacc
     3181 gaccgcgtcg gttctctcga tcattggctt cttctccgat ttgcccgccg ctttcggcac
     3241 ggcggcatcc ggggatagcg attgcggggc aaccgccttc gttcggcttt gctccgtagc
     3301 cgtatctttc atacctgctg cagcagaatt gccggaagcc ggagctgtag ctgtaactgc
     3361 cttcgtttcg acattttccg ctgacggcag gtcagtggag tctgccaacc gcaggctgcc
     3421 atccatgctt aggagcagtg gcgtcacgga cgacggcggt tctgccgatg acgtcgcgct
     3481 ttctggcgga gcaggcgttg gcggcgcagt cggtgagaca cgcgacgagg cagcaacgac
     3541 cggctccgca agctgggcta ccggagccgc ctcctggcga agaaaggcat agccgccgac
     3601 tatcgcaatc aggagaatca atccagcggc accgaggacg atcggcaaga gcggagttcc
     3661 gggcttcggc aacgaacgaa cgtcgtttgg cagattggta ccggtaccat acgttgcccg
     3721 ccgggcgtca aggtcctgga gaacctggtt gatcagactc atggccggcc cttcaacacg
     3781 atcatcccca caaccacccg gtacgatgcg cgccgtcggt gtccttgcgc gccagtctga
     3841 cgtggcgcat cttggccatc tggcggcctt cgccgaaaac cgacagtaat gatttatgcg
     3901 ccagaatgtt gatcaatcgg ggtgtgccgc cactggcacg atgcagcgcg cgcaatgcgg
     3961 cgggggaaaa aagaccctct ccccgatagc cggcaacccg caaacgatgt gccacatagt
     4021 ttgcgatttc gcttttcttc aggccgccca agcggtagtg aaaggcgatg cgctggagca
     4081 gctggcgcac ttccggacga tgcagcttga cgtcgagttc cggctgaccg aaaagaacga
     4141 tctgcaacag cttgcgcttt tcggtttcaa ggttggatag aagccgcaat gcttccagcg
     4201 tttcaaccgg catcacttgc gcctcgtcga tgcaaaccac gacccgcttc ttccctctcg
     4261 caaagccaag cagcgcatgg ttgatccgac gcaccaggtg gtactggtcc aggccggccg
     4321 catccttgat acccaactct tccgccagcg ccagaaatag cgtatccgca ttgagattgg
     4381 ggttgggcag atacgccgtc acccagctct cgtccaaggt ttgcaacaag cgccgacaga
     4441 gcaaggtttt cccggtgccg acttcaccgc tgatcttgac aaagccctct ccctccgaga
     4501 gcgccagcag caaggtgttg agcgcttcct ggtgatactg gacagagtag atgaaactgg
     4561 tgtccggcgt gatgccgaaa ggcagctcgg tcaggccgaa gtgtttcaga tacaacgcag
     4621 gctccctggc gatggcacca tcactgtccc ggctgaggcc gtgccaagcg cggatcgagc
     4681 ttctgaattc gcccttgagt ctcgaccagg tcgtccttcc agcttgatgc attgcggatg
     4741 atcgtcggtt tgatgaggat gaccagttcg cgctttctca gatgactgtt gcgcgtcccg
     4801 aacaacagtc cccagcctga agagcccgtc gtacccggca agccgttgga atccgaagcc
     4861 tgttcctgct tcatcaagcc cccgatggcg acgatgttgc cgtcctgaac gcgtaccacg
     4921 ctgtctgttt cattgaccgc gctgttggcc agcggcaggg tgaactcacc gagggcgcca
     4981 agatcgatga ccgtctgttt cgccttgacc aggctgatcg acgggtgaac atgcagaacg
     5041 atattgtcgt cttcgtcgat ttgcggcgtt acatccaggg cgatgcccga aaagaatggt
     5101 tgcagcgtta tcgaaggcgt tgtcacatta cctgtgcccg atgtggtcac cgtcgaactg
     5161 acgttggtta cgaagaactc atcgacaccc accttgagca cagccatctg gttgttgata
     5221 ctggcaattc tcgggctaga caagacctga acattgcctt gtgtttcgag gaagttcagc
     5281 aatgccgcaa agttggcgct ctgaaaagca agtccgaaaa acccgctacc cagcttggtc
     5341 gctgcaacaa gaccacccac gccgggcaat acgccggtgc ctggcgccag tatcgccccc
     5401 gctccgcttt gcgctaaatc gccgctgtca aatccggcac gatttgtcgt gttcagaacg
     5461 gttcccggct gagccgcgcc ataggcaaaa cggttattcg ttccaccaaa ctggctccag
     5521 ttgacacccg cctgaaactc ctcgttcagc tccacttcga tgatcttggc ttccagcatt
     5581 acctgccgct cgatgatcaa ctgagtgatg cgcaaatact gctcgacgga gcggatatcg
     5641 gacggaaacg ccttgaccag gaccacgccc gaaccaggat tgacgatcac cgcgcgcccg
     5701 ttgtccgagc cgacaatact ggtcaaggcc gtggtcagat cacgccagaa atcgttgtgc
     5761 tgcgatgtcg atacctggct gctcggcgga cctgccgtat tcccggctgg cgagacaggt
     5821 agcgcggtat ttgtcactgt tgcgccgccg gcctgaggtt gtatggcggt catcgtattt
     5881 gaagtcacgc tcacttcgga ctgccccttg cgttttccgg ccaggtagtt gagctggaag
     5941 atacgcgtct gcagcgtatt gggctgaata tagatacgcg ttccctggaa cttgaactca
     6001 tagccataca gctcccgcag ggtctccagc gcttcacgca ctgtgacact tttcagattg
     6061 accgtcacgt tgccggcaac ttccggcgca accagcatgc tgtatcgcgt cccgctcacc
     6121 aaagccatga agacctgcgt cgccggtgcg ttactcacgg ccaggtcgaa acgcggctcg
     6181 acgcttttcg ccgatgcggg caattcgagc tgtattggag gcaacatggc ctggttgagt
     6241 gcctcgtccg caccccgcgg cttggagctc accgcacctt gcatttccgt cccgatcagg
     6301 tcgaaggtgg ttccaggtgt cttctgatgc gtagcgcacc cgaccaccag cacgaccgcc
     6361 gccagcggaa atatcaattt gaacttcatg gtttgccccc accctgcgca cgtttggcag
     6421 cagatgattt tcttatgatg ttagtttttt cgattcccgg cgtcagcatc aagcgctcga
     6481 tccctcccgg cccttcaagc accgcctcgc gctcactcag cttgatcagg cggctgtcgc
     6541 cgtacagctc gcccaacttg acctgttgcc ccccgatgac ggcactcggc ttgcctttct
     6601 tcggaatcat gaccgactgt agcacagggc cggttacccc tcccgcatcc gcgtcgggag
     6661 aagaaatgcc ggccggcggg cgggtcggat ccgacagcaa cgcctgcgcc cggacgctca
     6721 ccatcagcgt gcatagcgtc aatatgaaga gcgcacgaat aacggagttc agacgatcag
     6781 ccatgttcga tccagactca aggtatagac agtcagcacc agcaccaacc tgggatgctg
     6841 ttcggcagaa agcgaaacgc tgctccacag cagtttcgat tgggatttct caagacgctc
     6901 aagatacgca gccagctcct ggtagctccc ttccagcctg atctcgacgc catgcttgaa
     6961 aaggcccgcc gaggaagcag ccagtttatc cgcggccttc gccgctcccg tttgttctgc
     7021 gtcagacttc ttctccagca gaggggatac cggcaggttc ttcaggctga gcaggcgcag
     7081 gcccgccttg cgcccgatca tgtcttccag caaggctggc acgcgttgcg gtggcaccaa
     7141 cgatccttct acggccacca gtcggtcggc aagtgctccc agttgcttct tcagtctgtc
     7201 caactcaatg cgcgcagcga catcgggatc ctggctcggg gacagcagca cggccatctg
     7261 ttgctgcatg ttggcgagtt ggacccgctg ctccagctca ctgcgttctg ccagttgtgc
     7321 gcgcttcatt gccgggtcga cgaagacgct gtagccaatc agaaagatgc cgccaagaac
     7381 agccgccgca accagccagc gctcgcgcaa ttgcagcgcg tcatagcgcg cggccagggc
     7441 gagccaggat gccttcatcg cttgccccct tggtgcgcag cgttgtccgc cccgcctgca
     7501 ttttccgagc gaagcgcgaa ctccacaaaa cgtggcaatc tgggcactcc ggattgctgc
     7561 cctccatccg acggggattc gctcgccacc gcagcaccac gctgattatt ctgatcctcg
     7621 ggatcgatgc ctcgcatctc caatgcggca aagcgacgcc cctggaacgc cggctcgctg
     7681 ctcaagcgct gcacataggc cggtagcttc gacggatcga gcatgcgtcc gcgaatttca
     7741 atttcctcgc cgccgaccgt caccagaaac ccggtcagcc ataaatcgct ttgcgacagc
     7801 cgggcaaaac cggccatcat ggcagaaaac cccgatatat tgcccaggct gcctgaatcc
     7861 aggaccgcca tgacttcttc acgcgtgacc agcatcgctt ttgcattttc cagctcagcc
     7921 gccaaagcgg gcgaaacgcg gcgctcggac attgtccggg tcaactcggc cagtttctcc
     7981 tgtccgtcag ccaattgctg ttgcgacacc gcggctactg cgctcttcct tttcgcatcg
     8041 aagtgcgccc aaagcgaaag cgccgtcatc aacaccagca gcgcggcagt acaaatcccg
     8101 agattgcgtg cgacgggcag ttcatggcgc ggccgcaacc gggcttcgta gagattgatc
     8161 tgctggctca cgctgcagtc tcggccgcgc tgcttgttcg caatgcggca ccaatcgcca
     8221 ggagattctt cgcctgacac tgcgtgtccc tcaattccgg aatcgcagga aaatcgatca
     8281 ccgacgaaaa gtccatctcc ttcaccggaa tgtaggtgtt ttcacccaga tcggcggcaa
     8341 gattctcgac actcggataa gttgccagca cgacttttga gattggaatg tggctgtact
     8401 gccgatcgaa gttatcaaga ctgcgttgca gctcaagcac caggcgctcc ttggtgcgtg
     8461 aacgctggtc cgcatcacct ccatcgagct ggagagtact gatttcgccg cttcgcacgg
     8521 caatcagctc gccgtgaaag ctcagcgtca acatcatgcc gttctcgtcg atgcgcagaa
     8581 ataccagtcc gcgattctcg tcttcaagca atgcggccac gttgcgctgg gccagttccg
     8641 gtatatcgac cgcatccagg ataatcttgg cttcctcgaa ggcgccaacg cgcgaacgga
     8701 ccgcctgctc ggcggcagag acgaccagaa cacccgccga acgaccgggt ggcataccca
     8761 ggattggaat atcgagcaca tcgatgcaag cgctatccag cggatagctc accgtctcct
     8821 tgagcgccca gcgcaacgcc tcccggcgtt cttccgctgg aacgggcggc gattcgagct
     8881 gcgtgagatt gtattcgccg ctacccatca aggtcgtaca ggcataggac ttgagctgac
     8941 gggcaacgcg caggcgctgc agggcatcac gctcacttcc ctccagcgcg aagctgtcca
     9001 gcagcctgac ttcgggccgg gaattgcgat cacgcacgac gtgtgccagt gtgacctgtc
     9061 cgctttgcgg aaggatggac agccagcctg gtttgcgcgg cgtcgagaaa aaccgaatca
     9121 ttcagtcgcc ttgttacaat tttgtttaaa gatacgcatt aaccttctaa ataacaaggg
     9181 tttttccaaa aacaatcagt aattttcccg gcgatagatc aggggggtct tgtagcgtcc
     9241 aaacgtggca attgctggag gaacaacccc caaccaggct ggcacggttg cggcaattgt
     9301 cgtcgtacca aaattgttgg cacccggcgc tttcagccac aaattgaaat cgcctgcaaa
     9361 gcctatggtt ggcgttgcac cttcccggta tcgccgagca ggcggagcga cagtggcaca
     9421 ggctcctgcg ccgctcaatc ccggactccc attatcccaa atgcaaactg gcagacctat
     9481 cggcgcggat gcaagagaca cacttacaga
//
LOCUS       DAORPM010000075         9482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1686_length_9482_cov_9.876101, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000075 DAORPM010000000
VERSION     DAORPM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9482
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_9482_cov_9.876101"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="actP"
                     /locus_tag="PLD53_05260"
     CDS             complement(<1..365)
                     /gene="actP"
                     /locus_tag="PLD53_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764712.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRC60352.1"
                     /translation="MMTKTIRNSPAILLGCLIAGVALAAGGDLGTTVKQATNWTAIGM
                     FGAFVIMTLFITKWAASRTKTAADFYTAGGGITGFQNGLAIAGDYMSAASFLGISGLV
                     FANGFDGLIFSIGWLVGWPV"
     gene            complement(362..679)
                     /locus_tag="PLD53_05265"
     CDS             complement(362..679)
                     /locus_tag="PLD53_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HRC60353.1"
                     /translation="MKEDELVARIAANPKYEKLVSMRSSYSIIMTILVMCIYYGYILL
                     IAYNKEFLAQKMGAGMVTSIGIPMGVGVIVLTILITNIYVRRANSEFDTLNGEIISEA
                     NKP"
     gene            1024..1710
                     /locus_tag="PLD53_05270"
     CDS             1024..1710
                     /locus_tag="PLD53_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC60354.1"
                     /translation="MHVLLIEDDSELSAGIAAFLAGKGDSVVVEADGMAADRLLQERS
                     FELVLVDVGLPGLGGYEIVRRIRERGQLIPVIMITARDALDDRIYGLDLGADDYLSKP
                     FELAELTARIRAVTRRSTHTVTHRVEFGPLSIDMDGRHASLADQPLILSNREWDLLTT
                     LAAADGHSVPKERLLADGSVNALEVYLCRLRRRLEVAGLNIRTVRGFGYRLEVASEVA
                     DKTWQTASTQ"
     gene            1756..3246
                     /locus_tag="PLD53_05275"
     CDS             1756..3246
                     /locus_tag="PLD53_05275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRC60355.1"
                     /translation="MNSGKEGSESGAVSKTREAGASLRRSLLHALVVPLTLALFTGVV
                     LGYQAAEKVVSTAYDQSLLNLATGIANRARVENGEITMGLTAEAEALLRTDTIDRIYF
                     RVRDDSGRIIVGDADLPPPEISPGALSANLSELPPSEIQARDQTKAGGLSTTFFDDHF
                     RGQAIRGVRFHPIVDEHGIYVTVAETLGKRHGAIRDLLVGFGLAVLLLLAAVAVVVRY
                     GISSGLASLHRLEAELAERSGQDLAPVDLSHVPLEIREVVRALNALLARLGEANLAQR
                     KFLQDAAHQLRTPLASLQVQIELLRSTRADPTISDNLRKSVKRVTRLANQLLALARAE
                     AGQHLIANASPVDLAKLIDDMLEEWLRIADEKKIDFGVQRDVSVIAGDPTLLREMIAN
                     LVDNALKFTPVGGQVTLRCACVGELLEIDVSDNGPGIPEHERERVFERFHRLPDATAA
                     GSGLGLPIAREIVAGHHGSIVITACPDDKGTCVKVRLPLNLSTVSV"
     gene            3411..4598
                     /locus_tag="PLD53_05280"
     CDS             3411..4598
                     /locus_tag="PLD53_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60356.1"
                     /translation="MSIRIATSVTAGLLLIAGTAFAQSKPPVAQFWMDVATNSMSIPG
                     MEDADTGAGGMFGGFFGGTKMGGGSPGQWLDTALYTRNKRAGTEGAHAIPPGMRMGNS
                     LPLIPVAGSQPVEGREDDTEKPKGKLMFYWGCGDKVRPGQPRTVDFSRASPDEWSRFM
                     TGRFAPDRGAKAVAGRSVWPNERDRQRVPNGASLAGDHAVSGEGVPAGLRFALRDAYD
                     FMPKIKMNISGDPKASVNVSWQSINAAEGYFLSAMGSKGENEMIIWSSSEQPDPGWGL
                     MDYLAPAQVKKLIGEKVVLAPSIQNCAIPSGIFGAAEGAMVRMIAYGPELNLAHPPRP
                     ANPKAPWDPEWTVRVRIKSTGMTVLGMDEQGGDRSQGKDRGTSPEHETPGLGGVIRGV
                     FGL"
     gene            4628..6121
                     /locus_tag="PLD53_05285"
     CDS             4628..6121
                     /locus_tag="PLD53_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRC60357.1"
                     /translation="MSIDTAFAVPRFVFDKQDLLTRLSRILPAQSLLVDEEDLRPYEC
                     DGLMAYRQLPLIVALPENEAQAQDILRICHALKVPVIPRGAATGLSGGAMPHRDGVVV
                     SLAKLKKIIKLDPLGRTAVVQPGVRNLAVSEAAAAHALYYAPDPSSQIACTIGGNVSE
                     NSGGVHCLKYGLTVHNVLRVRGLTVEGEVIEFGNAALDAPGYDLLALMNGSEGMLALI
                     TEVTVKLTPKPELAQVVLASFDDVTKAGDAVANIIAAGIIPAGLEMMDRKAIHAVEPY
                     VNAGYDLDAEAILLCESDGTPEEVDEEMARMTEVLKHSGASGIRVSQNETERLKFWAG
                     RKAAFPAVGRITPDYLCMDGTIPRKNLARMLIAIAEMEQRYGLRCANVFHAGDGNLHP
                     LIMYDANVPGQLEVATSFGGEILELSVALGGSITGEHGVGVEKIAQMCAQFSPAELEV
                     FHALKRAFDLDGLLNPGKAIPTAARCREYRLIRSAANCTDNMAAGVG"
     gene            6118..7209
                     /gene="glcE"
                     /locus_tag="PLD53_05290"
     CDS             6118..7209
                     /gene="glcE"
                     /locus_tag="PLD53_05290"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HRC60358.1"
                     /translation="MSINVNSCLERWTDRLQAASADRTRLCLRGSGSKDFYGQSLTGE
                     LFDTREYSGIVAYEPTELVVTARCGTPLAELEEALAEKGQSLSFEPPHFGTGATVGGC
                     VASGLSGPRRAQAGALRDFVLGVKILNGQGELLSFGGQVMKNVAGYDVSRLMAGSLGT
                     LGLIVEVSLKVLPVAVAERTLLFELAQDKALDLLNRWGGQPLPISASAWRENILHIRL
                     SGATAAVSSACRTLGGQMLAPDEAMPFWVGLREQRHAFFDGDAPLWRVSVPSTSAVLD
                     LPGAQLVEWGGALRWLRGGASLTDQAVRPVAEQVGGHATLFRADETKKAEFGVFQPLA
                     APLARIQKNLKQAFDPQGIFNPGRMYSEF"
     gene            7223..8494
                     /gene="glcF"
                     /locus_tag="PLD53_05295"
     CDS             7223..8494
                     /gene="glcF"
                     /locus_tag="PLD53_05295"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HRC60359.1"
                     /translation="MQTNLADFIKDTPEGREADSILRSCVHCGFCNATCPTYQLLGDE
                     LDGPRGRIYLIKQMLEGQPVTRRTQVHLDRCLSCRSCETTCPSGVNYHRLLDIGRNVI
                     EQRVARSIAEKATRFLLCQALPQPLIFKPAVKLGQWMRPLLPVVLADKVPAQSSGASK
                     RYPACQSSARNRRMIALAGCVQPTLTPNTNAAAVRILGKLGIELFEAAGAGCCGALRY
                     HLNAQEQALDDMRRVIDAWWPHVEGNQSGNVEAIVMTASGCGAHVREYGELLKHDPAY
                     AEKASRISALTLDISQVLAVEQERLVELMQLKGKVGVGESRKLAFHSPCTLQHGQKIR
                     GVVESLLVAAGYELTMVADSHFCCGSAGTYSITQPELSGRLRKNKLAALGAGSAEVFV
                     TANIGCQTHLQSGTATPVRHWIELIDERLGD"
     gene            8714..>9482
                     /locus_tag="PLD53_05300"
     CDS             8714..>9482
                     /locus_tag="PLD53_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60360.1"
                     /translation="MSPKKYTKIAALFSCSLIPLVAGATGLGEMRVTSQLGDRFHAEI
                     PLLNPSSSVVPSCFNLTTRTTEAISDVPWLQGAQIALKSSPPRLVISTRRQVSDPIVQ
                     LAIYTGCGTYLTRHFTAMLSPPESLKSLPVVAPMRTDASLTTPKTTPGKHVSGSQRAT
                     RNEGVRTKQAGETAADMARRLYPDDPRAQEVFVQRMDAFKPEWLVAEAREETRPVAAE
                     PHAPAPPVRPVATARPKPKPKPVVISRKKTETKTDSTD"
BASE COUNT         1920 a         2656 c         2877 g         2029 t
ORIGIN      
        1 accggccagc cgaccagcca gccgatcgaa aagatcaggc cgtcgaagcc gttggcgaac
       61 accaggccgg aaataccaag gaaggacgcg gcggacatat agtcgccggc aatcgccagg
      121 ccgttctgga aaccggtaat gccgccaccc gccgtgtaga agtcggccgc tgtcttggtc
      181 ctgctggccg cccacttggt aatgaacagg gtcatgatga cgaaagcgcc aaacatgccg
      241 atggcggtcc agttggttgc ctgcttgacg gtcgtgccga gatcgccacc ggccgccagg
      301 gcgactcccg cgatcaggca accgagcagg attgctgggc tgttgcgtat cgttttcgtc
      361 atcatggttt gttggcctcg ctgatgattt cgccattgag cgtgtcaaat tcgctgttgg
      421 cccggcgcac atagatgttg gtgatgagga tcgtcagaac gatcacgccg acgcccatcg
      481 ggatgccgat cgaggtcacc atgccggcgc ccatcttctg cgccagaaac tccttgttat
      541 aggcgatcag caggatgtag ccatagtaaa tacacatcac caggatggtc atgatgatgc
      601 tgtagctgga gcgcatgctt accagctttt cgtactttgg gttggctgca atacgcgcaa
      661 ccagttcgtc ttctttcatt ctgcctcctc gcaaagggac ggttagtcga gtcattcggg
      721 atgactgcga aggccacggt aggaatttta gcttacgctg cgcttacaga catataaatt
      781 attgcgtaaa tacaatagct tacagaatac ttgcccattt cttaatggtg gcttacgaga
      841 cgatggtttt cggattggtt tccggcacct gtaccgtgcg taagagcggc gaatcgaatt
      901 gacgccccac gctgccaact actgtcgtcg cgtacaatca ctggtacagc tgctgtcgtg
      961 ctgtcgtccg cgttttcctg acacgatctc cttccctttt tccgttgtcg atcgaggtgt
     1021 ttcatgcatg tactgttgat agaagatgat tcggaattgt ctgctggaat tgctgccttc
     1081 cttgccggaa agggcgatag cgtcgtcgtg gaagccgacg gcatggctgc cgatcgtttg
     1141 ttgcaggaac ggagctttga actggttctc gtcgatgttg gacttccggg gttgggtggc
     1201 tatgaaatag ttcgacgaat acgggaacgc ggccaactga ttccggtgat catgattact
     1261 gcgcgagacg cgctcgacga tcgaatctac ggtcttgatc ttggcgcgga cgactacctg
     1321 tcgaaaccct tcgagcttgc cgaattgact gcacgcatcc gcgcagttac ccgtcgtagc
     1381 acccataccg tcacacaccg cgtcgaattt ggcccattgt caatcgacat ggacggccgg
     1441 cacgcctcct tggctgatca gcctctgatt ctttccaatc gggaatggga tctgctcact
     1501 acccttgctg cggccgacgg gcactccgtt cccaaggaac gtctgctggc cgacggaagc
     1561 gtcaacgccc tggaggtata cctctgccgc ctgcgtcgac gtctggaagt tgcgggcctg
     1621 aacattcgca cggtgcgtgg tttcggctac cgtctggaag tcgccagcga ggtcgcggac
     1681 aagacttggc aaacagccag cacccaatga ttcggcgaga tgtatgccga atcaagtctg
     1741 atatcctgat gtcgtatgaa tagtggtaag gaaggttctg aatccggtgc ggtctccaag
     1801 acacgggagg cgggggcgag ccttcggcgc agtctactgc atgcgttggt agtccccctc
     1861 acgctcgcac tgttcacggg agtcgtgctc ggatatcagg cggcagaaaa ggtggtcagc
     1921 accgcatacg atcaaagctt gttgaacctc gcgaccggta tcgccaaccg tgcccgggta
     1981 gaaaatggcg aaatcacgat ggggctgact gcggaggcag aagcactgct tcgcacggat
     2041 acgatagatc gcatctattt cagggttcgc gatgattcag gcagaatcat cgtgggcgac
     2101 gctgatttgc caccgccgga aatttccccc ggcgctcttt cggccaacct ttctgaattg
     2161 ccgccatcgg aaattcaggc gcgcgaccag accaaggccg gcggactcag cactactttt
     2221 tttgacgacc acttccgtgg ccaagcaata cgcggcgtgc gctttcatcc gatagtcgat
     2281 gagcatggaa tctacgttac cgttgctgaa acactgggaa agcgccatgg cgcgattcgt
     2341 gatctgctgg ttggctttgg ccttgcggtg ctactgctgc tcgcagccgt agcagtagtc
     2401 gtgcgttacg gcatcagctc tggactggca tcgctacacc gcctggaagc agagcttgcc
     2461 gaacggtccg gccaggatct tgcgccggtt gatctgagtc atgtgccact cgaaattcgc
     2521 gaggtcgtgc gcgcattgaa cgcccttttg gctcgcttgg gagaagcgaa cctggcccaa
     2581 agaaagttct tgcaggatgc tgcgcatcaa ttgcgaaccc cgctcgccag ccttcaggtg
     2641 cagatcgagt tgctgcgcag cacacgggca gatccgacca tttcagacaa tctgcgcaaa
     2701 tccgtaaagc gcgtaacccg actggccaat cagctgctgg cactggctcg cgccgaagct
     2761 ggtcagcatc tgatagccaa tgcatcgccg gtagacctgg ccaaactgat cgatgacatg
     2821 ctggaggagt ggctgcggat cgccgacgag aagaagatcg actttggcgt gcagcgcgat
     2881 gtttcggtga tcgctggcga cccaacgctg ctcagggaga tgattgcaaa tctggtcgac
     2941 aacgcgctca agttcacgcc ggtcggaggg caggtcaccc tgcgctgtgc gtgcgtcggc
     3001 gagctgctcg aaattgacgt cagtgataac ggtcccggaa ttccggaaca cgagcgtgaa
     3061 agggtgttcg agcgctttca ccgcctgccg gatgcaacgg ccgcgggtag cggcctgggt
     3121 ttgccgatcg ctcgcgaaat cgttgccggc caccacggtt caatcgtgat caccgcctgc
     3181 ccggacgaca agggaacctg tgtgaaggtc cgcctgccct tgaatctgtc aaccgtttca
     3241 gtctagattc ccgctgcggg cggaattggc cgtccgtcgg gtcgttccat tatgaactgg
     3301 aatgggcgga ttcgcgtgaa tgtggagttc gtgcgctcct tcgcctctgc aaacaagagc
     3361 cggacagctt cctgatctat actgagactt tcattgtgaa tgaggcaagc atgtctattc
     3421 gtattgcaac atccgttact gccggtctgc ttctgattgc cggaacggcg ttcgcccagt
     3481 cgaagccgcc tgttgcccaa ttctggatgg acgttgccac caacagtatg tcgattcccg
     3541 gtatggaaga tgccgacacc ggtgcgggcg gcatgttcgg ggggttcttc ggcggcacca
     3601 aaatgggtgg cggatcgcca ggtcagtggc tggatacggc gctctacacg cgtaacaagc
     3661 gggccggtac tgagggtgcc catgccattc cacccggcat gcggatggga aacagcctgc
     3721 ctttgattcc ggtagccggg agtcagcccg ttgaaggacg tgaagacgac acggaaaagc
     3781 cgaagggcaa gctgatgttc tactggggct gtggagacaa ggtgcgcccc ggtcagccgc
     3841 gaacagttga tttctcccga gcgagtcctg acgaatggtc ccgtttcatg accggtcgct
     3901 ttgcccctga ccggggtgcc aaagctgttg caggtcgatc ggtgtggccc aacgagcgtg
     3961 accgtcagcg cgtgccgaac ggtgcttccc tcgcaggcga tcacgcggtc agtggcgaag
     4021 gcgtcccggc agggcttcgc ttcgcattgc gcgatgccta cgatttcatg cctaaaatca
     4081 agatgaacat cagtggtgat ccgaaagcct cggtcaacgt gagctggcaa tccatcaatg
     4141 cggctgaagg ctacttcctt tcggccatgg gctccaaggg tgaaaacgaa atgatcatct
     4201 ggagttccag cgagcagcca gaccctggtt gggggttgat ggactacctg gcgccagcgc
     4261 aggtcaagaa attgattggt gaaaaggttg tgctggcgcc cagcattcag aactgtgcca
     4321 ttccatccgg catattcggt gctgccgagg gcgcaatggt gcggatgatt gcctacggac
     4381 ccgaactcaa tctggcccac ccgccgcgtc cagccaatcc gaaggcgcca tgggatccgg
     4441 agtggaccgt gcgcgtgcgc atcaaatcga ccggaatgac cgttcttggc atggatgagc
     4501 agggtggtga tcgctcccaa ggtaaggatc ggggtacttc gccggagcat gaaacgccgg
     4561 gtcttggcgg tgtcatccgg ggcgtcttcg gtctttaggc acatttgctt gagtattgcg
     4621 attccgcatg agcattgaca ccgcattcgc cgttccacgc ttcgtcttcg acaagcagga
     4681 tttgttgacg cgactgagca ggattctgcc ggcgcagagt ctgctggtcg acgaggagga
     4741 tttgcgcccc tatgagtgcg atggcctgat ggcctaccgc caattgccgc tgatcgttgc
     4801 tctgcccgag aatgaggcgc aggcgcaaga cattctcagg atttgccacg cgttgaaagt
     4861 gccggtcatt ccccgcggag cagccaccgg cttgtcgggt ggtgcaatgc cgcatcggga
     4921 cggagtcgtg gtgtctctgg ccaaactcaa gaagatcatc aagctggatc cgttggggcg
     4981 aactgccgtc gtacagcctg gcgtgcgtaa tctggccgta tccgaggcgg cagcggcaca
     5041 tgcgctctac tatgctccgg acccttcctc ccagattgca tgcactatcg gcggcaacgt
     5101 atccgaaaat tctggaggcg tgcattgcct gaaatacgga ttgacggtgc acaacgtgct
     5161 gcgtgtgcgc ggcctgaccg tcgagggaga ggttattgaa ttcggaaatg ccgcgctgga
     5221 tgcgcctggc tacgacctgc tggcgctgat gaacggttcc gaaggcatgc tggcgttgat
     5281 taccgaggtt acggtcaagc tcacgcccaa accggaactc gcgcaggtcg tactcgcttc
     5341 gttcgacgac gtgacgaagg caggcgacgc ggtggccaat atcattgcgg ccggcatcat
     5401 ccccgccggt ctggaaatga tggacaggaa ggcgatccat gccgtcgagc cgtacgtcaa
     5461 tgccggttat gacctggatg ccgaagccat cctgctctgt gagtccgacg gaacgccgga
     5521 agaagtggac gaagaaatgg cacgcatgac ggaagtgctc aagcacagcg gcgcttccgg
     5581 gattcgcgtc tcgcaaaatg aaacggaacg gctcaaattc tgggccgggc gaaaagcggc
     5641 ttttccggca gtcgggcgaa tcacgccgga ctatctgtgc atggacggga ccattccgcg
     5701 caagaatctg gcacgtatgt tgattgccat cgccgagatg gaacagcgct atggattacg
     5761 ctgcgccaac gtttttcacg ctggagacgg caacctgcat ccgctgatca tgtacgacgc
     5821 caacgtgccg gggcaacttg aagtggcgac gtcctttggc ggcgagattc tggagctgtc
     5881 ggtcgcgctg ggcggctcga ttaccggtga acacggcgtc ggtgtcgaga aaatcgcaca
     5941 gatgtgcgcc cagttttccc cggcggaact ggaggtcttc catgcactga agcgtgcatt
     6001 tgacctggac ggcttgctca atccgggcaa ggcgattccc acggctgcgc gctgtcgcga
     6061 ataccggttg atacgtagtg cggccaactg taccgacaac atggcggcgg gggttggatg
     6121 agcatcaatg tcaattcatg ccttgagcgc tggaccgatc gcttacaggc cgcatcggcc
     6181 gaccggaccc gcttgtgcct gcgtgggagc ggcagcaagg atttttacgg ccagtcgctg
     6241 acgggcgagc tgttcgatac gcgggagtat tccggtatcg tggcttatga gccgaccgag
     6301 ctggtcgtga ccgctcgctg cgggacgcca cttgccgaac tggaagaagc actggctgag
     6361 aaggggcagt cactgtcttt cgagccccct cacttcggca cgggagcgac tgttggcggt
     6421 tgcgtggcaa gcggcctgtc tggtccgcgc cgtgcacagg ccggggcgct tcgcgatttt
     6481 gtgctgggtg tgaaaatact taatggtcag ggcgagttgc tcagtttcgg cgggcaggtg
     6541 atgaagaatg ttgccggtta cgatgtgtcg cgactgatgg ccggcagcct gggcacgctg
     6601 ggcctgatcg ttgaagtctc gctcaaggtt ctgccggttg ctgtcgcaga gcggacgctg
     6661 cttttcgaac tggcgcaaga caaggcgctg gatttgctca atcggtgggg cgggcagccg
     6721 ctgcccatct cggcttcggc gtggcgagag aatatccttc atatccgcct ctcgggggct
     6781 acggcggcgg tgagctcggc atgcagaacc ttaggtggtc agatgcttgc acccgatgaa
     6841 gcgatgcctt tctgggtcgg cttgcgtgag cagcgccatg ctttctttga tggcgatgca
     6901 ccgctgtggc gcgtttcggt cccctcgaca agcgcggtac tggatctgcc gggtgcgcaa
     6961 ctcgtggaat ggggtggtgc gctgcgctgg ctgcgcggtg gagcctcgtt gaccgatcag
     7021 gcggttcgtc cagtagccga gcaggtcggc ggacacgcaa cgttgtttcg agccgacgag
     7081 acaaagaagg ccgagtttgg cgtatttcag ccgctggccg cacccttggc acgcattcaa
     7141 aagaatctga aacaggcttt cgacccgcaa ggcattttca atcccggtcg catgtattcc
     7201 gaattctgat cgccgactga ccatgcaaac aaatcttgcc gattttatca aggacacgcc
     7261 ggagggcagg gaagccgaca gcattttgcg cagctgcgtt cactgcgggt tctgcaacgc
     7321 cacctgtccc acctatcagt tgctgggcga cgagctggac ggaccgcgcg gacgcatcta
     7381 tctgatcaaa cagatgctcg aaggccagcc ggtcacccgg cggacgcaag tccaccttga
     7441 tcgctgcctg agctgtcgct cctgcgaaac gacctgtcca tcgggagtga actatcaccg
     7501 attgctcgat atcggacgca acgtcataga acaacgcgtt gcgcgttcca tcgccgaaaa
     7561 ggcgacacgt tttctgttat gccaggcact tccgcaaccc ttgatcttta agccagcggt
     7621 gaaacttggt caatggatgc gcccgttgtt gcccgtcgtt ctggccgaca aagtgccggc
     7681 gcagtcgtcg ggtgccagca agcgctatcc agcctgccag tcctcagcgc gcaaccggcg
     7741 catgatcgcg cttgcgggct gtgtgcagcc gaccctgacg cccaatacca acgccgcagc
     7801 agttcgcatt ctgggcaagc tgggtatcga actgttcgag gcggcgggtg ccggctgctg
     7861 cggtgcactg cgttaccact tgaacgctca ggaacaggcg ctggatgata tgcgccgggt
     7921 gatcgacgcc tggtggccgc atgtcgaagg caatcaatcc ggcaatgtcg aagcgatcgt
     7981 gatgactgcg tcgggctgtg gtgcgcatgt ccgcgaatac ggcgagctat tgaaacacga
     8041 tccagcctat gctgaaaagg cgtcgcgtat ttcggcgctg acgcttgata tttcccaagt
     8101 gcttgccgtc gaacaggaaa gactcgtcga gttaatgcaa ttgaaaggca aggtcggcgt
     8161 cggtgaaagc cggaagcttg cttttcactc accttgtacc ttgcagcatg ggcaaaaaat
     8221 ccgcggtgtt gtagaatcgc tgctggtcgc cgccggttac gagctcacaa tggtggctga
     8281 ttcacacttt tgttgcggtt cggcaggcac ttactcgatc acccagcccg aactttccgg
     8341 gcggctgcgc aagaacaaac tggctgcgct cggagcaggt tctgcagaag tgttcgttac
     8401 agccaatatc ggttgccaga cgcacttgca gagcggtaca gcgacacccg tgcggcactg
     8461 gattgaactc attgacgagc ggctcggcga ctagaagccc cctcaggaat cgcacgttca
     8521 gcgccacgcg tttgtgctgc ttggagctgg atcaaacgct ctcgcagctt gtgcgtggtg
     8581 ccgaccagaa tccaagaaaa aggggcatgg tgtcgaattt ttcgtttccc atgctgagaa
     8641 ctgagcatta tttaacatca acaattttgt cccttcagca caatcgttca gcacgaaatt
     8701 cgagatatgt gaaatgagtc ccaaaaaata caccaagatt gctgcactgt tctcctgttc
     8761 ccttattccg ttggtggcag gcgcaaccgg cctgggcgaa atgcgtgtca caagccagtt
     8821 gggagatcgc tttcacgcag aaataccgct cctgaatccg tcttcttcag tcgttcccag
     8881 ctgcttcaat ctgactactc ggacgacaga ggccatttcc gatgtgccgt ggcttcaggg
     8941 cgcgcaaatt gcactgaagt cgtcgccacc gcgcctggtg atcagtacgc gtcgacaagt
     9001 gtctgaccct attgttcaac tggccatata cacgggttgc ggtacttacc tgacgcgtca
     9061 tttcacggca atgctttctc cgccggaaag tctcaagagc cttcctgttg tcgccccaat
     9121 gcgcacagat gcgagtttga ccacccccaa gacaacccca gggaaacatg tcagtggctc
     9181 tcagcgcgct acgcggaatg agggagtgcg cacaaagcag gctggcgaaa cagccgccga
     9241 catggcgcgc aggctgtacc cagacgatcc gcgggcacag gaggtttttg tccaacggat
     9301 ggatgcgttc aaacctgaat ggctagttgc agaggcgcga gaagaaacgc ggcccgtagc
     9361 tgctgaaccg catgccccgg cgccgcccgt gcgcccggtg gctacggctc ggccgaagcc
     9421 caagcccaag cccgttgtca tctcgcgcaa gaagaccgaa acgaagacag acagtactga
     9481 tc
//
LOCUS       DAORPM010000076         9444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1693_length_9444_cov_11.883587, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000076 DAORPM010000000
VERSION     DAORPM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1693_length_9444_cov_11.883587"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /gene="map"
                     /locus_tag="PLD53_05305"
     CDS             <1..706
                     /gene="map"
                     /locus_tag="PLD53_05305"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRC60361.1"
                     /translation="VTTGALDKLCHDYMVDVQNCIPAPLDYAPAGYKPYPKSICTSIN
                     HQVCHGVPGDRQLKNGDIVNLDITTIKQGYHGDTSRMFHVGEVSIQAKRLCEVTFECL
                     WLGIAQVRAGAHLGDIGHAIQQYAEKSGYSVVREFCGHGIGARFHEDPQVVHYGKAGT
                     GIELKANMIFTIEPMINAGKAAIRELADGWTIVTKDHSLSAQWEHTILVRETGFEVLT
                     LSEGARKPPEWVKLSS"
     gene            703..3303
                     /locus_tag="PLD53_05310"
     CDS             703..3303
                     /locus_tag="PLD53_05310"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRC60362.1"
                     /translation="MKLDLDDRNALLARYKSRLQSGQAAIFERYLAEADAPRLLHERC
                     QLVDDLLRELWTELCFPQSLALLAVGGYGGGKLWPASDVDLLLLLPGMPDAMLTEKLE
                     QLVGLFWDIGLEIGHSVRTVEECLNEAAGDLTVRTALVEARLLIGNEQLFSKFNTEFR
                     SNLNPQEFFHAKRMEQDERYLRFQESPYSLEPNCKESPGGLRDLQCILWIAQAAGYGA
                     SWQDLERHGFITHDEAQGLERRELFLQRLRIELHLHVGRREDRLLFDYQSVIAKQLGF
                     TGIPTRRASEQMMQKYFQTAKTVTQLNVILLQNIGSAIFPSPKQAALAVNERFQCTNQ
                     LLDIAHENVFNQTPAAIFEAFLLMQEHAELKGMTARTIRSLWRAGVLIDDDFRQDPEN
                     RMRFIEIFKQPKGVLHELRRMNQFDILGRYLPNFGAIVGQMQHDLFHAYTVDQHSLQV
                     LRNLRRFSETEYSHEYPFCSQLISGFDRPWVLYIAALFHDIAKGRGGSHSELGAVDAQ
                     EFCTAHGLYAEDSELIVWLVRQHLVMSSVAQKQDISDPDVLGNFAANVVDERRLIALY
                     LFTVADIRGTSPKVWNSWKGRLLEQLFSQTRRLLRAGGKAPVTQGLVEERQEEALRLL
                     RYFAVPEAAQEQLWKQLDTVYFLRHSAEEISWHARALHYRSDSAQPVVKARINPHGDG
                     IEVMAYTKDQRYLFARLVGFFSRAGYNIMDAKIHTTRNGYALDSFMLQDVSGRDSDRN
                     MISYIEHELIDRLMQQAPPEVPASGRISRQVKHFPIKPLVTIEPLVTLQPDEKGVLFV
                     LSIIAADRPGLLYIVAMALAAHGANLHTAKIFTLGERAEDTFLISGGDLGEQSSRIKL
                     ETELLDLLKL"
     gene            complement(3310..3846)
                     /locus_tag="PLD53_05315"
     CDS             complement(3310..3846)
                     /locus_tag="PLD53_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRC60363.1"
                     /translation="MAIRTVLRMGDPRLLEQARALESFASPELLALIADMEETMHHLD
                     GAGLAAPQIGVALRVVIFGFDENPRYPEAGSVPYTVLINPVLTPLSDAIEEGWEGCLS
                     VPGLRGSVPRWQHLHYRGFDPFGQVIERSVEGFHARVVQHECDHLDGVLYPMRMTDFS
                     RFGFVDSFLQDSTASTSD"
     gene            complement(3824..4579)
                     /locus_tag="PLD53_05320"
     CDS             complement(3824..4579)
                     /locus_tag="PLD53_05320"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HRC60364.1"
                     /translation="MLAVIGGSGLSQLSNLDVSHREVVRTPYGEPSGAVTFGEICGRP
                     IAFLARHGYGHTIPPHEVNYRANIWALCKRGAVGIISVASVGSIRADLKPGDLVIPHQ
                     IIDYTWGRKSSFHEGPECTLTHIDFTEPYDNALREQLLSAAGAAGIPVSTVAVYAATQ
                     GPRLETAAEINRLERDGADVVGMTGMPEAALAREMAVPYAAISVVANYAAGRADSRQG
                     ISFEAIQDVLHESMGHVRTIIEKMVGCGDPNRP"
     gene            complement(4588..>5014)
                     /locus_tag="PLD53_05325"
     CDS             complement(4588..>5014)
                     /locus_tag="PLD53_05325"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HRC60365.1"
                     /translation="NPLLLCVMSGGVPFAGHLMTQLNFPLDFDYLHVTRYGQDTAGGA
                     LSWRSAPWTPVKGRTVLIIDDILDEGLTLAAIVERIKQLGARNCYTAVATDKLNGKQK
                     PIKADFVALTVPDRFVFGFGMDVRGAWRNLPAIYAVKDE"
     assembly_gap    5015..5114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5115..5809)
                     /gene="galU"
                     /locus_tag="PLD53_05330"
     CDS             complement(<5115..5809)
                     /gene="galU"
                     /locus_tag="PLD53_05330"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRC60366.1"
                     /translation="MKKVKKAVFPVAGLGTRFLPATKASPKEMMPVVDKPLIQYAVEE
                     AVEAGISDMIFVTGRTKRSIEDHFDKAYELESELALKNKTEMLEFVRNMLPRNINCIY
                     IRQTEALGLGHAVLCAKPVVGDEPFAVLLADDLLVGQPPVMKQMTDTYDYYRCSVVGV
                     LDVPRAETASYGMVRSTSVAERVERIEGIVEKPKPEDSPSTLGVVGRYILTPRIFHHL
                     EKVKPGSGGEIQLT"
     gene            complement(5814..7859)
                     /gene="ligA"
                     /locus_tag="PLD53_05335"
     CDS             complement(5814..7859)
                     /gene="ligA"
                     /locus_tag="PLD53_05335"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRC60367.1"
                     /translation="MNGRERALELRAEIEKHDHQYYVLDAPLISDDEYDRLFRELQAL
                     EQAHPELLTADSPTQRVGGKVLDGFASVRHVVPMLSIRTETDTTASGAFAFDARVRRE
                     LKLSEDDPPVEYAAELKFDGLAINLRYEDGVLVSAATRGDGETGEEVTANVRTVGQIP
                     LRLRTEEPPVVIEVRGEIYMKRDDFLRFNARAEAAGKKMLVNPRNGAAGSIRQLDSHI
                     AASRPLSFYAYGLGEVKGWKVPVTHSAVLDALSNFALPVCAERAVLHGPQPLADFHRR
                     IGERRASLPFDIDGVVYKVNALALQTQLGFVSREPRWAVAHKYPAEETNTEVLGIDIQ
                     VGRTGALTPVARLAPIFVGGVTVTNATLHNEDEIRRKDIRVGDTVVVRRAGDVIPEVV
                     RVLPEKRPIMAAEFVMPGFCPICGSHVVRSENETVARCSGGLFCPAQRKQALIHFASR
                     RALDIEGLGDKLVEQLVDGGVVRTPADLYKMGLLALVSLERMADKSASNLLAAIEKSK
                     QTTLARFIYALGIRNVGETTARDLARHFGSLDRILAADEDALMQVDDVGPVVAQCLRE
                     FCAEPHNLEVVEQLRAAGVVWAENPVEKTTQPVEDSVLAGKVFVLTGTLPSMTRDEAR
                     AMIDAAGGRANATVSKKTDYLLAGAEAGSKLEKALKLGVAVIDEAQFRLMLGQAQEE"
     gene            complement(7877..9187)
                     /locus_tag="PLD53_05340"
     CDS             complement(7877..9187)
                     /locus_tag="PLD53_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HRC60368.1"
                     /translation="MTELQTGLIGLGVAAVVGVAAYNKWQEYRHRKLAEQVLNNHHAD
                     VLLDESEVDVSGAAHAANALKADVEREAPANTAAEPSLDFKENLPAFLMPGERVEPVF
                     HDDQQHSEPDMARQEPVVEPASSSAVLEPEPSPKVETVTDPISGMSRLPDVEKSAKDG
                     TEYAHLLSPLIDYVAAFETVEPLPANQILDAQRAVLMRIRKPVHWIGYNEFTREWEPI
                     IDDGDIEYRRFRIGVQLVDRQGPLDDSDLSVFHIAMQDLAADLMAIVDLPARQPALDA
                     AAKLDAFCASVDIQIGINVISQGQAFPGTKLRALAEAAGMLADGEGHFVRSDDEGNVL
                     FVLHNQEAQGFSVESMKTMSTHGITFLLDVPRVSHGERVFNQMVDLARRFSEALRGSL
                     VDDNHRPLSASALDPIRSQVGRYQAVMAEHGLPAGGPLTRRLFS"
     gene            complement(9196..>9444)
                     /locus_tag="PLD53_05345"
     CDS             complement(9196..>9444)
                     /locus_tag="PLD53_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60369.1"
                     /translation="LNPAPFCLLDEVDAPLDDTNTERFCAMVRRMSANTQFLFISHNK
                     IAMEMAQQLIGVTMQESGVSRIVAVDMDEALRMREQIV"
BASE COUNT         2037 a         2846 c         2539 g         1922 t
ORIGIN      
        1 cgtcaccacc ggagcgctgg acaaactgtg ccacgactac atggtcgatg ttcagaattg
       61 catacccgca ccgcttgact acgcgcccgc cgggtacaag ccgtatccaa aatcaatctg
      121 cacctcgata aatcaccagg tttgccatgg cgttcccggc gacaggcagt tgaagaatgg
      181 cgacatcgtc aatcttgaca tcaccaccat caagcagggc tatcacggtg ataccagccg
      241 catgtttcat gttggtgaag tctcgattca ggcgaaacgg ctttgcgagg tcacttttga
      301 atgcctctgg ctcggcatcg ctcaggttag agccggtgcc catctcggcg atatcggcca
      361 tgccattcaa cagtatgccg aaaaatccgg ctactcggtc gtgcgtgaat tctgtggaca
      421 cggtatcggc gcacgattcc acgaagaccc acaggtcgtg cactacggca aggccggtac
      481 cggaattgag ctgaaagcca acatgatctt caccattgaa cccatgatca acgccggcaa
      541 ggcggcgatc cgtgagctgg ccgatggctg gacgatcgtt accaaggatc acagcttgtc
      601 ggcgcaatgg gaacatacca ttcttgttcg cgaaaccgga tttgaagttc ttacgctctc
      661 cgagggcgcg cgcaagccgc cggaatgggt caagctgtcc tcatgaaact tgacttagac
      721 gatcgcaacg ccctactcgc ccgctacaaa tcacgcttgc agtctggtca ggcggctatt
      781 ttcgaacgct acctggccga agcggatgct ccacgcttgc tccacgagcg ctgccaactg
      841 gtggacgacc ttctgcgtga attgtggacg gagctgtgct tcccgcagtc cctggcgcta
      901 ctcgctgttg gtggctacgg gggcggaaaa ctgtggccgg catccgatgt tgacttgctc
      961 ctgttgcttc cgggaatgcc cgacgcaatg ctcaccgaaa aacttgagca actggttggc
     1021 ttgttctggg atatcggact ggagataggc catagcgttc gcacggttga ggaatgcctc
     1081 aatgaggccg caggcgacct gacggtcagg acggcacttg tcgaagcccg actgctcatc
     1141 ggaaatgagc aactgttctc gaagttcaac actgaattcc ggtccaatct caacccgcag
     1201 gagttcttcc acgccaaacg gatggagcaa gacgagcgtt atctgcgttt tcaggaatca
     1261 ccctacagcc tcgaaccgaa ctgcaaggaa agcccgggtg gcttgcgcga cctgcaatgc
     1321 atcctgtgga ttgcccaggc ggcaggctat ggcgcgagct ggcaggatct ggagcgccac
     1381 ggcttcatca cgcacgatga agcacaaggc ctggagcgca gggaattgtt tttgcaacgc
     1441 ttgcgcatcg agctacacct ccacgtaggg cgccgtgaag atcgccttct gttcgactat
     1501 cagtccgtga tcgcaaagca attggggttt accggaatac cgacccggcg cgccagcgag
     1561 caaatgatgc agaagtattt tcagacggcg aaaacagtta cccagctcaa tgtgattctg
     1621 ttacaaaaca tcggcagcgc gatctttccc tcgccgaagc aagccgcgct ggcggtcaat
     1681 gagcgcttcc agtgcaccaa ccagttactc gacattgctc acgaaaacgt tttcaaccaa
     1741 acccctgccg caatttttga agcgtttctg ctcatgcagg agcatgccga actgaaaggc
     1801 atgacggcac gcacaattcg ctcgctctgg cgggcaggcg ttctgatcga tgacgatttc
     1861 cgccaggatc ccgagaacag gatgcgtttc atcgagatct tcaagcagcc gaaaggggtg
     1921 ctgcatgagc tgcgacgcat gaaccagttt gacatcctcg gccgctacct gcccaacttc
     1981 ggcgccatcg tcggccagat gcagcacgac ctgttccacg cctataccgt cgaccagcac
     2041 agcttgcagg tcctccgcaa cctgcgccga ttctcagaaa cggaatacag ccacgaatac
     2101 ccgttctgca gtcagctgat cagcggcttt gatcgaccct gggtgctcta cattgccgcc
     2161 ctgttccacg atatcgccaa ggggcgcggc ggctctcact cggagcttgg tgcagtcgat
     2221 gcacaggaat tctgtactgc acacggcctg tatgcagaag acagcgagct tattgtctgg
     2281 ctcgtgcgcc aacaccttgt catgtcgagc gtcgcccaga aacaggacat ctccgacccg
     2341 gacgtgctgg gcaattttgc ggcaaacgtc gttgatgagc gccgcctgat tgcactctac
     2401 ctgttcacag tcgccgacat tcgcggcacc agtccgaaag tctggaatag ctggaaaggc
     2461 cgcctgcttg aacaactgtt cagccagaca cgccggctgc tgcgggcggg cgggaaggcc
     2521 cccgtgacgc aaggactcgt cgaggagcgg caagaggaag cactgcggct tcttcgctat
     2581 tttgcggttc cggaggccgc acaggaacaa ttgtggaagc agctcgatac agtgtatttc
     2641 ctgcgccatt cggccgaaga gatttcctgg cacgcgcgcg ccctgcacta ccgttcggac
     2701 agcgcgcaac cggtggtcaa ggcccgcatc aacccccatg gcgacggcat tgaagtcatg
     2761 gcctacacca aggatcagcg ctatcttttt gcccgtctgg tcggcttttt cagccgggca
     2821 ggctacaaca tcatggacgc caagattcat acgacgcgca acggttatgc gctggacagt
     2881 ttcatgctgc aagacgtcag tggccgtgac agcgaccgca acatgatcag ctacattgaa
     2941 catgaactga tcgaccggct catgcagcaa gcgccacccg aggtgccggc aagcgggcgc
     3001 atttcgcgcc aggtcaagca tttcccgatc aagccgctcg taacaataga gcccttggtg
     3061 acgctgcagc ccgatgaaaa aggcgtactc ttcgttctct cgatcatcgc tgccgaccgt
     3121 cccggtctac tgtatatcgt agccatggcg ctagcggctc atggcgccaa cctccatact
     3181 gcgaagattt tcacgctagg tgaacgcgcc gaagatacat tcctgatcag cggcggggat
     3241 ctcggcgagc aaagcagccg gatcaagctg gaaacggaat tgctggatct cttgaagctc
     3301 tgaatgaatt cagtccgaag tgctggccgt tgagtcttga agaaatgaat caacgaaccc
     3361 gaaacggcta aaatcagtca tccgcatcgg gtatagaact ccgtcaagat gatcgcattc
     3421 atgctggacg acccgtgcat gaaatccttc gaccgaacgt tcaatcacct gcccgaaagg
     3481 atcgaaacct cgatagtgca gatgctgcca gcgcggcact gaaccgcgga gtcccggcac
     3541 ggacaggcat ccctcccagc cctcctctat cgcgtcagag agcggcgtca gaacaggatt
     3601 gatcaacacc gtgtagggta cgctgccagc ttccggatag cgcggattct catcaaatcc
     3661 aaaaatcact acgcgcaggg cgacgcctat ctgtggtgca gcaagacccg caccatcgag
     3721 atggtgcatg gtctcttcca tatcggcaat cagggcgagc agttccggtg aggcgaagga
     3781 ctcgagcgcg cgcgcctgtt ccaacaggcg cggatcaccc atcctaagga cggttcggat
     3841 cgccacagcc caccatcttc tcgatgatcg tacgcacatg acccatcgac tcatgcagca
     3901 cgtcctgaat cgcttcaaag ctgattcctt gacgactgtc ggcccggccg gccgcataat
     3961 tggccaccac gctgattgct gcatagggca cggccatttc gcgcgccaag gcggcttccg
     4021 gcataccggt catacccact acgtcggcac cgtcccgctc aaggcgattg atctcggcgg
     4081 ccgtttccag tcgtggccct tgtgtcgccg cgtaaacagc aaccgtgctc actggaattc
     4141 cagcggctcc agcggctgaa agcagttgtt cacgcaaggc attgtcatag ggttcggtga
     4201 aatcgatatg cgtgagcgta cattccggcc cttcatggaa actggatttt cgcccccacg
     4261 tgtaatcgat gatctgatgc ggaataacca gatcgccagg cttgaggtca gcccgaatac
     4321 tgccgaccga ggcgaccgaa atgatcccga cggcaccgcg cttgcagagc gcccagatat
     4381 tcgcccgata gttcacttca tgcggcggta tcgtatggcc ataaccgtgc cgtgccagaa
     4441 aagcgatggg acgaccacaa atctcaccaa aggttacggc acccgacggc tcgccatagg
     4501 gtgtgcgcac cacttcgcga tgagagacat cgaggttgct cagttgcgaa agaccactcc
     4561 ccccgataac ggccagcatt tcactcatca ttcgtccttc acggcataga ttgccgggag
     4621 gttccgccac gcaccacgaa cgtccatgcc aaaaccgaac acgaagcggt ccggaaccgt
     4681 cagcgcgaca aaatccgcct tgatcggctt ttgtttgcca ttcaacttgt cggtcgccac
     4741 agcggtataa caattcctcg cgccgagttg cttgatgcgc tcgacgatgg cagccagcgt
     4801 cagaccctcg tcaaggatgt cgtcgatgat aaggacggtg cgtcccttga ccggagtcca
     4861 aggtgcagag cgccatgaca atgcgcctcc ggccgtatcc tgaccgtaac gtgtgacgtg
     4921 caagtagtca aaatcgagag ggaaattcaa ttgcgtcatc agatgtccgg caaaaggcac
     4981 gccgccgctc atgacgcaca gcagcaaagg attgnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnngtcaat tgaatctcgc cgccagaacc cggctttacc ttctccagat
     5161 gatgaaaaat acgcggtgtc agaatgtacc ggcccaccac gcccaaggtc gagggggaat
     5221 cctcgggttt cggcttttcg acgatccctt cgatgcgctc gacgcgctcc gcgactgaag
     5281 tcgacctgac catgccatag ctggctgtct cggcacgcgg gacatcaagc acgccgacca
     5341 ctgaacaccg gtagtaatcg taggtatccg tcatttgctt catcaccggc ggttggccaa
     5401 cgagaagatc gtccgccagg agtacggcaa agggctcgtc gccaactacc ggtttggcac
     5461 aaagcaccgc gtggccaagc ccaagggctt cggtctggcg gatgtagatg cagttgatgt
     5521 tccttggcag catgttgcgg acgaattcga gcatttccgt cttgttcttc agcgccagct
     5581 cgctctcgag ctcataggct ttgtcaaaat gatcttctat cgatcgcttt gtccgtccgg
     5641 tgacgaaaat catgtcgctg atcccggctt cgaccgcttc ctcgaccgcg tactggatca
     5701 aaggcttatc gacaacgggc atcatttctt tcggactggc cttggtcgcc ggcaaaaatc
     5761 gcgtgccgag gccggcgacg gggaaaaccg ctttttttac ttttttcatt atttcattcc
     5821 tcttgcgctt gccctaacat taaccgaaat tgggcctcat cgatcacagc cacacccaac
     5881 ttcagtgctt tctccagctt ggaacccgct tcagcgccag ccagaaggta gtcagtcttc
     5941 tttgagactg tcgcgtttgc cctgcctcca gccgcatcaa tcatcgccct ggcctcatcg
     6001 cgcgtcattg atggcaacgt tccggtcagc acgaatacct tgcccgccag gacgctgtcc
     6061 tcaaccggct gcgttgtctt ttcaaccggg ttttcggccc agacgacacc agccgcgcgt
     6121 aattgttcga cgacctccag attatgcggt tcagcgcaga attcgcgcaa acactgagct
     6181 actacaggac cgacatcatc aacctgcatc aaggcgtctt cgtcagccgc aaggattcga
     6241 tcaagcgaac caaagtgtcg cgccagatcc ctggccgttg tctccccgac attgcgtatg
     6301 cccagcgcgt agatgaaacg cgccaacgtc gtttgtttgc ttttctcgat cgccgccagc
     6361 agattgcttg ccgatttgtc ggccatgcgc tccagactga ccaaggccag caagcccatc
     6421 ttgtacagat cggccggcgt tcgcacaacg ccaccatcaa ccagttgctc gaccagttta
     6481 tctcccaggc cttcgatatc cagggctcgg cgcgatgcaa agtgaatcag cgcctgcttg
     6541 cgctgtgcgg ggcaaaacaa tccgccactg caacgcgcca cggtttcatt ctcactccga
     6601 accacgtgcg acccgcaaat cgggcagaat cctggcataa cgaactcggc tgccatgatc
     6661 ggtcgttttt cgggcagcac acgcacgact tccggtatca catcgccggc acgacgcacg
     6721 acgaccgtat cgccaacacg tatatccttg cgccggattt cgtcctcatt gtgcaacgtc
     6781 gcgttggtca ccgtgacgcc gccgacaaaa atcggcgcga gtcgcgctac cggcgtcaac
     6841 gcaccggttc ggcccacttg tatatcaatg ccaagcactt cggtgttggt ttcctcggcc
     6901 gggtatttgt gcgctaccgc ccagcgtggc tcgcgcgaaa caaagccgag ctgtgtctgg
     6961 agcgcgagcg cattgacctt gtatacaacg ccgtcgatat caaatgggag gctggcgcgt
     7021 cgttccccaa tgcgccgatg aaaatcggcc aacggctgcg gcccgtgcag tacggcgcgc
     7081 tcggcgcaaa cgggcaatgc gaaattcgaa agggcgtcga gaacagcgct gtgcgtaacc
     7141 ggcaccttcc agcctttgac ttcgccaaga ccgtaggcat agaaggaaag cggccggctg
     7201 gcggcgatat gcgaatccaa ttgacgaatg cttccagcgg cgccgttgcg cgggttaacc
     7261 agcattttct tgccggccgc ctcagcacgc gcgttaaagc gcaggaaatc gtcgcgtttc
     7321 atgtaaattt cgccgcgcac ttcaatcacg acgggtggct cctcggttcg caaacgcaac
     7381 ggaatctgcc cgacggtgcg gacgtttgcg gtaacctctt cgcccgtttc accatcgcca
     7441 cgagttgcgg cgcttaccag cacgccgtct tcgtatctca ggttgatcgc caaaccgtcg
     7501 aacttcaact cggctgcata ctcgaccggc ggatcatctt cggaaagctt gagttcgcgg
     7561 cgcacgcgtg cgtcgaaggc gaaggcgccg ctcgctgtcg tgtcggtttc ggttcggatc
     7621 gacagcatcg gcacgacatg acgcacggac gcgaagccat cgagcacctt gccacctacg
     7681 cgctgcgtgg gcgaatccgc cgtcagcagt tcgggatgcg cctgctcaag cgcctgcaac
     7741 tcgcgaaaca agcggtcgta ttcgtcatcc gaaatcagcg gtgcatcaag aacatagtac
     7801 tggtggtcgt gtttctcgat ctcggcgcgc aactcgaggg cgcgttcgcg gccgttcatg
     7861 cgtggagaat cccagctcag gagaacaagc gccgagtcag cggcccgcca gccggcaggc
     7921 catgctcggc catcaccgcc tggtaccgtc ccacctggct gcgaatcgga tccaatgcac
     7981 tggcggaaag cgggcgatgg ttgtcgtcca ccaatgatcc acgcagcgct tcggagaatc
     8041 gccgcgccag atcaaccatc tggttgaata cgcgttctcc gtgcgaaacg cgcgggacgt
     8101 ccagcaaaaa ggtaatgccg tgcgtgctca tcgtcttcat cgactcgacg gaaaaccctt
     8161 gcgcttcctg attgtgcagc acaaagagca cgttgccctc gtcgtcgcta cgtacgaaat
     8221 gcccttcacc gtcagccagc attcctgccg cctcggcaag cgcacgcagc ttggttccgg
     8281 ggaaggcctg cccctggctg atgacattga ttccaatctg aatatcgacg ctggcgcaaa
     8341 acgcgtcgag ctttgccgcc gcgtcaagcg ccggctggcg ggcaggcagg tcaacaatgg
     8401 ccatcagatc tgcggcaaga tcctgcatgg caatatgaaa gacagaaagg tcgctgtcat
     8461 ccagtggccc ctggcgatcg accagttgta cgccaatcct gaaacgacga tactcaatgt
     8521 cgccgtcatc gatgatcggc tcccactcgc gcgtgaattc gttgtaaccg atccaatgaa
     8581 ccggttttcg gatgcgcatc aggacagcac gttgcgcatc aagaatctgg ttggccggca
     8641 acggttcgac cgtttcaaaa gctgccacgt aatcgatcag aggagacagc aaatgcgcgt
     8701 actcggtacc gtccttcgca ctcttttcca cgtcaggaag tcgcgacatg cctgaaattg
     8761 ggtcggtaac ggtttcgacc ttcgggcttg gttcaggctc caatactgcc gaagaggaag
     8821 ctggctcgac gacaggctcc tggcgcgcca tgtcaggctc agagtgctgc tgatcatcgt
     8881 ggaaaacggg ctcgacccgc tcgccgggca tcaggaaagc gggcaggttt tctttgaaat
     8941 ccagcgacgg ctctgctgcc gtattggccg gtgcttcgcg ctcaacatcg gctttcagcg
     9001 cattggcagc atgagcggca ccggagacgt caacctccga ctcgtcaagc aagacgtctg
     9061 catgatgatt gttcaagacc tgctccgcca gcttgcggtg tcggtattcc tgccatttgt
     9121 tataggctgc cacgccgaca acggcagcaa cacccaagcc aatcaaacca gtttgcagtt
     9181 cagtcatgtc tattcctaaa cgatctgctc gcgcatgcgc agcgcttcgt ccatatccac
     9241 cgcaacgatg cgcgaaaccc cggattcctg catcgttacg ccgataagct gctgcgccat
     9301 ttccatcgcg atcttgttgt ggctgatgaa cagaaattgc gtgttggccg acatacgtcg
     9361 caccatcgca caaaaacgtt cggtattggt atcgtccaac ggcgcatcca cttcgtcaag
     9421 caggcagaac ggcgccggat tgag
//
LOCUS       DAORPM010000077         9369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1718_length_9369_cov_8.159652, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000077 DAORPM010000000
VERSION     DAORPM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1718_length_9369_cov_8.159652"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLD53_05350"
     CDS             complement(<1..213)
                     /locus_tag="PLD53_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRC60370.1"
                     /translation="MQAKLSIVALSVAAAFALSACGQKDAPPPVAAPATPAAPAAKPE
                     VVVKLGHVAPMTGPQAHLGKDNENGAV"
     gene            333..1628
                     /locus_tag="PLD53_05355"
     CDS             333..1628
                     /locus_tag="PLD53_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60371.1"
                     /translation="MTSLSRVRFLIHQAARKLFSMLPQGQRHGIYRSFVDCDPAPNER
                     LVLKIADTQEELEACFKLLHDAYVSSGFMTPDPSGMRVTIYHALPTTTTLCAKLNDQV
                     IGTISLIRESAIGFPLQRIFDLSEVREKAGYIAEVSALAIHPKFRRTGGTILFPLMKF
                     MYEYCTTFFDTRHLVIAVNPRHIEMYESLLFFRRLNANMVENYDFVNGAPAVGATLDL
                     KHAPEIMRKAYAKKPLRRNLHHYFLETKLPNIQLPNRRFFTTNDPVLTPELINYFFNE
                     RTTVFAELSERKKTLLHLIYDLPAYQKVLPPTLNAPGDIKNRRHQRFSVKCPASLNLS
                     SDERPIKIEIDDVSRYGFHARTSRPVPTNKFLDAVIQLGTHEISRLTVQAIQGSDAED
                     KHFCGFRIGEPDLIWRKFVNALYEGHTHTDLDQATRFLP"
     gene            complement(2129..3037)
                     /locus_tag="PLD53_05360"
     CDS             complement(2129..3037)
                     /locus_tag="PLD53_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRC60372.1"
                     /translation="MKLLSRILAATDLSAPARHAVERAFHLAASANCELYILNAIELD
                     SLDSLRELLGGDVLAVKAALKCDAHDRLDQLTGNAAINRGVVPHTCVADGNPFATIAA
                     EAEVLNADLVILGARGESFLRHTLLGSTAARLLRKSSRQPVLVVKQPPHEAYRSVLIA
                     VDFSPVSLSAIRMAKQVAPGADLVLLHAFELPYEGKLTFAGVDEQLIRQYVTAGSESR
                     RKRLHDLAVAAGLTTSEYSARIVHGDPAQQIIAMEQEVNADLIVVGKHGSHIAEELLI
                     GSVTKHVLAESQCDLLVMCDPHDAPE"
     gene            complement(3131..3328)
                     /locus_tag="PLD53_05365"
     CDS             complement(3131..3328)
                     /locus_tag="PLD53_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRC60373.1"
                     /translation="MESFFEAITETQKTSLEAAASFTDTYLIVVERIAQLNIEVARAA
                     FEKSSEMALLCLESSLSKVKW"
     gene            complement(3415..3825)
                     /gene="rnk"
                     /locus_tag="PLD53_05370"
     CDS             complement(3415..3825)
                     /gene="rnk"
                     /locus_tag="PLD53_05370"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HRC60374.1"
                     /translation="MTHLNFALAHNCPSESQGLQAPIVGTADYLRLRQYVLSDKLVDE
                     LDRAIVVETEQVPEDVVRMYARCTYVDQRIGTQREIELVYPDESDPAMGKISVLTPVG
                     SALIGLRVGQEIAWEFPDGSMRCLKVASVTQRAR"
     gene            3942..4844
                     /locus_tag="PLD53_05375"
     CDS             3942..4844
                     /locus_tag="PLD53_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRC60375.1"
                     /translation="MADLNLMAIFARVVEAGSFSEAARRMGCSRSAVSKAVAKLEKSL
                     GAHLLNRTTRYLSLTEIGAAVAEHCSRILDEATEAENLVGSLNSEARGVLRVSASVAF
                     GTLHVAPALAKFLPRHPKLKIDLSITDRWVNLAEEGYDVAIHVTGDPQPNLVARPLAP
                     VRRKLCATPDYFQRKGIPQTPADLVAHNCLDYTRSGEPGRWRFTGPEGEIAVPVNGPL
                     HVDDDEALSQAVLGGLGVGLLPTFIIGKDLQNGNLQAVLSEYIPVERHVYAMYLPTRH
                     LPSKVRVFIDFMISHIGEEPYWDR"
     gene            complement(4801..5007)
                     /locus_tag="PLD53_05380"
     CDS             complement(4801..5007)
                     /locus_tag="PLD53_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60376.1"
                     /translation="MLGVEGYSLLTGKQGLIGYLFNQLENGEPGSETVSLDLSVTKGV
                     FAARLNLTEQTIDPSTAPRRYGKS"
     gene            complement(5007..5321)
                     /locus_tag="PLD53_05385"
     CDS             complement(5007..5321)
                     /locus_tag="PLD53_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60377.1"
                     /translation="MQGLRSNTIVSAYQRYQRHPVSCEIDGELLTGNYWIAGMILVVS
                     TATGGASRQLANSEPEDLAKILLKDLVLANRAGMLAAVRVAEEKRMRDIPPANIETHC
                     IT"
     gene            complement(5329..5559)
                     /locus_tag="PLD53_05390"
     CDS             complement(5329..5559)
                     /locus_tag="PLD53_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HRC60378.1"
                     /translation="MFHEFRDLISELKTTDRYFQILFEKHNTLDQRIKNMESRLEPGT
                     PDEIDVLKKKKLAIKDELYVLLQKISLSRIKR"
     gene            complement(5588..6931)
                     /gene="gdhA"
                     /locus_tag="PLD53_05395"
     CDS             complement(5588..6931)
                     /gene="gdhA"
                     /locus_tag="PLD53_05395"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRC60379.1"
                     /translation="MRYQRFEEFIQQVAARNPGQPEFLQAVTEVIESLWPYIAQHPTY
                     AEHGLLDRLVEPERVIMFRVSWVDDHGEVQVNRGYRIQHSSAIGPYKGGIRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGRSPGEVMRFCQAFVSELFRHVGSE
                     TDVPAGDIGVGGREVGFMAGMMKKLSNRADCVFTGKGLSFGGSLIRPEATGYGTVYFA
                     EQMLKQQGRGFEGLRVSVSGAGNVAQYSVEKAMALGAKVVTVSDSSGTVVDESGFTPE
                     KLAELMEVKNHLYGRVSDYAKRVGAAFHPGLRPWRVPVDVALPCATQNELDGEDAKTL
                     VRNGVMCVAEGANMPSTADAVKVFESNGVLYAPGKASNAGGVATSGLEMSQNAMRLSW
                     QREEVDSRLHEIMCNIHEACLNYGRGTDGKVSYVNGANIAGFVKVADAMLAQGVI"
     gene            7276..8178
                     /locus_tag="PLD53_05400"
     CDS             7276..8178
                     /locus_tag="PLD53_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRC60380.1"
                     /translation="MHDINDMLFFAHVVKFRSFSEAARRLDVSKSRVSKAVARLESEL
                     GVRLLHRSTRSLSLTDVGESYFEHCDRILEELNQADSTISRLHQEPRGKLKISAPVAF
                     STMHVASALPDFMSRYPDLTVDLTISDRLVDLADEGYDIALRITWEPGQNLVARQLAP
                     IRRKICGSPSYLARQGVPLAPEDLVKHNCLDYTFMNTQGVWHLNGCNGNVAIPVSGTL
                     RINDDEALSQAVLGGLGLAVLPTFIVGKDLQAGRLVEVLPGYVPTERFIYAVHLPNRH
                     LPLKVRAFIEFLLNRFGPTPYWDR"
     gene            8335..>9369
                     /locus_tag="PLD53_05405"
     CDS             8335..>9369
                     /locus_tag="PLD53_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRC60381.1"
                     /translation="MSNMALPAATLDDKYTATTGRVFLTGTQALIRLPMLQRERDLAA
                     GLNTAGFVSGYRGSPLGNLDLGLWKAKAHLAEHHITFQPGINEDMAATAVWGSQQVGL
                     FPGAKYDGIFGLWYGKGPGVDRCGDVFRHANAAGSSKYGGVLVIAGDDHAAKSSTLPH
                     QTEHIFKALLMPVLYPANVQEYLDYGLHGWAMSRYSGCWVAFKALADTVETSASVRVD
                     LAAVNIVFPTDFALPPDGLNIRWPDPPLVQEARLLNFKLYAALAYARANQLNRVIIDS
                     PTPRLGILTAGKSYLDVRQALDELGIDDALAAEIGIRLYKVGMVWPLEPEGVRHFAEG
                     LEEILVVEEKR"
BASE COUNT         2028 a         2782 c         2522 g         2037 t
ORIGIN      
        1 cacggcgccg ttttcgttat ccttacccag gtgcgcttgc gggccggtca tcggcgccac
       61 gtggccgagt ttgacgacga cttccggctt ggctgctggc gcggcgggcg tcgccggggc
      121 ggctaccggt ggcggcgcat ctttttggcc gcaggctgaa agcgcaaaag cggcagcaac
      181 ggagagggca actatagaaa gcttggcttg catcggtgtt tctcctgatc tctgggggta
      241 aaaatgtgtt ttgattgtcc ggaggtgcag aatacatgtc aataacaagc tgctatgata
      301 aaaatcacag ccaactcaat taggcgatac cgatgacctc cctttctcgc gtccgctttc
      361 tcattcacca ggcggcacgc aagctttttt ccatgcttcc ccaagggcag cgtcatggca
      421 tttatcgctc ttttgtcgac tgcgacccgg caccgaatga gcggctggta ttgaaaattg
      481 ccgacaccca ggaagaactg gaagcctgct tcaaactgct ccacgacgcc tacgtcagca
      541 gcggcttcat gacaccggac ccgtccggca tgcgcgtcac catttaccac gccctgccga
      601 ccaccacgac cctctgcgcc aaactaaacg atcaggtcat cggcacgatc tccctgatcc
      661 gcgaaagcgc catcggcttt cctctgcagc gcatttttga cttgtccgaa gtccgcgaaa
      721 aagccggcta cattgccgaa gtatcggcgc tggccattca cccgaaattc cgacgcaccg
      781 gcggcaccat cctcttcccg ctgatgaagt tcatgtatga atattgcacg acctttttcg
      841 acacgcgcca tctggtcatc gcggtcaacc cgcgccacat cgaaatgtat gagtcgctgc
      901 ttttcttccg ccggctgaat gccaatatgg tcgagaacta tgattttgtg aatggcgccc
      961 cggcggtggg tgcgacgctg gacctcaagc acgccccgga aatcatgcgc aaggcttatg
     1021 ccaaaaagcc cctgcggcgc aatctgcacc actatttcct ggaaaccaaa ttacccaaca
     1081 tccagctacc gaaccggcgc tttttcacca ctaacgaccc ggtgctgacg cccgagttga
     1141 tcaactattt tttcaacgag cgcaccacgg tttttgccga actcagtgag cgcaagaaga
     1201 cgttgttgca tctgatctac gatctgccgg cttaccagaa agtactgccg cccaccttga
     1261 atgcgcctgg cgacatcaaa aatcgccggc atcagcgctt ctcggtcaaa tgtccggcca
     1321 gccttaatct ttcaagcgac gaacggccga tcaaaatcga gatcgatgac gtttcgcgct
     1381 atggctttca tgcccgcacc agccgacctg tgccgaccaa caagttcctc gacgcggtta
     1441 tccagctcgg cacccacgaa atatcgcgtc ttacggtgca ggcgattcaa ggctcggacg
     1501 ctgaagacaa acacttctgc ggctttcgca tcggcgaacc cgatctgatc tggcgtaaat
     1561 ttgtcaacgc gctatacgaa gggcatacac acaccgacct ggaccaggcg actcgcttcc
     1621 tgccgtagtc tgttgaaata aaggcatcca accgtctttc aaatggccag attaccgcag
     1681 tgaagtgatg cacgttctct ggaccttaag tcgggaacag gaaagttcgc tccctccact
     1741 tcaagggagg gcaggggtag tcaaatacca tactgcctcc tgtcgactta cactcaaagg
     1801 tggaaggtgg cgggcgccga aactgaattt gcctaaaggc gataagtcgc caagagcccg
     1861 tttaatgtgt tctttccaag gaagcaatcc agagtccgag tcagtctggc gggtgacgtt
     1921 agctgcggag ccaagccgtc aaacgagcat ttcgcccgct gctcggcacc gtccacatga
     1981 aagtctcttt caccttcagg ggtgacggca agcatcatga tcgttagctg gaactgtatt
     2041 tttcggccga ttcggacaac cgttcccagt gaaaattgtc agcgcaagta tggcattcaa
     2101 gtttcaatca acatattaat catggcaatc attccggtgc atcgtgcggg tcgcacatga
     2161 ccagcaaatc gcattgcgat tccgccaaca catgtttagt tacgcttcct atcagtaatt
     2221 cttcggcaat gtgtgaaccg tgtttgccga cgacaatcag gtcggcgtta acctcctgct
     2281 ccatagcaat gatctgctgc gcgggatcgc cgtgaacgat ccgtgccgag tattccgatg
     2341 ttgtcaggcc ggcagccacc gccagatcgt gcaagcgttt gcgtcggctc tcgctacctg
     2401 ctgttacata ttggcgaata agctgttcgt caacacccgc gaatgtcagc ttgccttcgt
     2461 agggcagttc aaaggcatgt agcagaacga ggtcggcacc tggggccact tgcttcgcca
     2521 tacggatcgc cgaaagcgac acgggtgaaa aatccacggc aatcaggact gaacgatatg
     2581 cctcatgggg tggttgtttc actaccagca ccggctgccg cgacgacttg cgcaacaagc
     2641 gggccgctgt cgaccccagc aaagtgtgac gcaaaaagga ttctccgcgt gccccgagaa
     2701 tcaccaaatc cgcattcagc acctcagcct cggcggcaat tgtagcgaag gggttcccat
     2761 cggcgacgca ggtatgcgga acgacgccac gattgatcgc tgcattacca gtcaactggt
     2821 ccaggcggtc atgggcatcg catttcaaag ccgccttcac cgctaacaca tcgcccccca
     2881 acagctctcg aaggctgtcg agtgaatcta actcgattgc gttcagaata tagagttcgc
     2941 agttagcact ggcagccaga tgaaaagcac gctcgacagc gtggcgggcg ggagcggaga
     3001 gatcagtggc ggcaagaatg cgcgaaagca gtttcatggc ttgtcctctt taggatggat
     3061 agacgcagcc ctcacagtat cttgccaggt gggactatcg aatcctcatg taacggcacg
     3121 accagcacaa tcaccatttt acttttgaaa gactgctttc caggcaaagc agagccatct
     3181 ctgaggattt ctcaaaagca gcacgggcta cctcaatatt gagttgagca atccgctcga
     3241 ccacgattag ataggtatca gtaaaactgg cagccgcctc cagggatgtt ttttgtgttt
     3301 ccgtgatggc ttcgaaaaat gattccattg aactctccat tgttgtaaaa catcatcgaa
     3361 acagactacc gttagctcca ggccctacca agcagcgcta ttcggttggc aggactatct
     3421 cgctctctgt gtgacgcttg cgacctttag gcaacgcatg gaaccatccg gaaattccca
     3481 agcgatttcc tgtcccactc ttagtccgat caacgcacta cccaccgggg tcaatacgga
     3541 aattttgccc atcgccggat ctgattcatc cggatagacc aattcgatct ctcgttgcgt
     3601 tccgatacgt tggtcgacat aagtgcaacg ggcgtacatg cgaaccacgt cctccgggac
     3661 ctgttctgtt tcaactacaa tcgctcgatc cagctcgtct accaatttgt cgctcagtac
     3721 atattgacgt aagcgtagat aatctgcggt acccacaata ggcgcttgca atccctgcga
     3781 ttcacttggg cagttgtgtg ccagggcaaa atttagatgt gtcataagtt gaaatcttaa
     3841 tgctcgcatc accatgacgc tagacagaca aatggcatca aaatgtagcc aaaatggcaa
     3901 caatcattgg ggcgtaacaa cagacaggag cgaaggcgtc gatggctgat ttgaatctga
     3961 tggcaatttt cgcccgcgtg gtcgaggcag gtagtttttc ggaagcggcg cggcggatgg
     4021 ggtgctcccg ttcggcggtc agcaaggctg ttgccaagct ggaaaaatcg ctcggcgcac
     4081 acctgctgaa ccgaacaacc cgctatctca gcctgacgga aatcggtgcc gcggttgcag
     4141 agcattgttc ccgcattctg gacgaagcaa ctgaagcgga aaacctggtc ggcagcctga
     4201 acagcgaagc gaggggggta cttcgcgtca gcgcttcggt tgctttcggc acgctccatg
     4261 tcgcacctgc tctcgccaag ttcttgccgc gccatccgaa actcaagatc gacctcagca
     4321 ttacggaccg ctgggtgaac ctggccgagg agggctatga cgtagccatc cacgttaccg
     4381 gcgaccccca gcctaatctg gtggcacgtc cgctggcacc agtccggcgg aaattgtgcg
     4441 caacccccga ctactttcag cggaaaggta ttccgcaaac tccggccgat ctggtcgccc
     4501 acaactgcct ggactacacg cgctccggag agccggggcg ctggcgcttt accggaccgg
     4561 agggtgaaat tgccgtaccg gtcaacggcc ctctgcatgt cgatgacgac gaggcgttat
     4621 cgcaagccgt cctgggcggt ctgggggtcg gtctgctgcc cactttcatc attggcaaag
     4681 atcttcaaaa cggcaactta caggccgttt tgtccgagta catcccggtc gagcgccatg
     4741 tctatgcgat gtatttgccg acgcgccacc tgcccagcaa ggtcagggta ttcattgatt
     4801 tcatgatttc ccatatcggc gaggagccgt actgggatcg atagtctgtt cggtcagatt
     4861 cagccgtgca gcgaaaacac ctttggtgac cgaaagatcc agggaaacgg tttcactgcc
     4921 cggctcgcca ttctccagtt gattgaacag gtagccgatg aggccttgtt tgccggtgag
     4981 cagggagtag ccttcaacac ccagcatcaa gtgatacagt gagtttcgat attggccggc
     5041 ggaatatctc gcatgcgctt ctcttccgcc acccggacgg cagcaagcat gcctgcgcgg
     5101 ttagccagca cgagatcttt cagcaggatt tttgccaaat cctccggctc gctgttggct
     5161 agctgccggc ttgcaccccc cgttgcagtc gaaacgacaa gaatcatccc ggcaatccag
     5221 tagttgccgg tgaggagctc gccatcaatt tcacagctaa cgggatgtct ttggtagcgt
     5281 tggtaagctg aaacgatcgt attgctgcga aggccttgca cgcggtgatc agcgcttgat
     5341 gcgactaagg cttatcttct ggagcagcac atataactcg tccttgatag ccagtttttt
     5401 ctttttcaga acgtcaatct catcgggcgt ccccggctcc aatcgggact ccatattctt
     5461 gatccgctga tcaagcgtat tgtgtttctc gaacaggatc tggaagtacc tgtctgttgt
     5521 tttcaattcg gaaatcagat cgcgaaattc atgaaacatg tgagcctccg tcggtattta
     5581 actattttta aatcacgccc tgcgcgagca tggcatcggc aaccttgacg aagccggcaa
     5641 tgttggcacc attgacgtag ctcaccttcc cgtccgtacc ccgaccatag ttcaggcagg
     5701 cttcatggat gttgcacatg atttcgtgca gacgggaatc gacttcctcg cgctgccacg
     5761 agagacgcat ggcgttctgg ctcatttcca gtccggaggt tgccaccccg ccagcattgc
     5821 tggccttgcc cggggcatag agcacgccat tactttcgaa gaccttcacg gcatcggctg
     5881 ttgacggcat gttggcgccc tcggccacgc acatcacgcc gttcctgacc aaggtcttgg
     5941 catcctcacc gtccagttcg ttctgcgtcg cgcacggcag ggcgacatcg accggcacac
     6001 gccacgggcg caagcctgga tggaaggctg cgccgacccg cttggcgtaa tcgctaacgc
     6061 gaccataaag atggtttttc acctccatca gttcggccag tttttccggc gtaaagccgc
     6121 tttcgtcgac gaccgtgccg cttgagtccg acacggtgac gaccttggcg ccaagggcca
     6181 tggctttctc gaccgaatac tgggcaacat tgccggcgcc ggaaacgctg acccgcaagc
     6241 cttcgaaacc gcgcccctgt tgcttgagca tctgctcggc gaaatagacc gtaccgtagc
     6301 cggttgcttc cgggcgaatc agcgagccgc cgaaactgag gcccttgccg gtgaacacgc
     6361 agtcagcacg attcgacagt tttttcatca tgccggccat gaagccgact tcacgtccgc
     6421 cgacgccgat atcgccggcc ggcacgtcgg tctcggaacc gacatggcgg aaaagttcgc
     6481 tgacgaaagc ctggcagaaa cgcatcactt cccccgggct gcggcccttc gggtcgaagt
     6541 cggaaccgcc cttgccaccg cccatcggca acgtggtcag ggcattcttg aaggtctgct
     6601 cgaaggccag gaacttgagg atggacaggt tgaccgatgg gtgaaagcgg atgccgccct
     6661 tgtaggggcc gatggcggag gagtgctgaa tacggtaacc acggttgacc tgcacctcac
     6721 catggtcatc gacccacgag acgcggaaca tgatgacgcg ctctggttca accaggcgat
     6781 cgagcaggcc gtgctccgca tatgtcggat gctgcgcgat gtacggccaa aggctttcga
     6841 tgacttcggt gacggcctgc agaaactccg gttgccccgg attgcgggcg gctacttgtt
     6901 gaatgaattc ctcaaaacgc tggtaacgca tcatctctcc ctggctcgtt gtatgtggtg
     6961 aattattcaa atcgtgtgga atctgccgcc tcgaaccaaa gcaatagccg ttggaattga
     7021 tagccaaaaa gcgacaataa gcaccccgat ctggtgcaag aagccagggt ggcgcgagga
     7081 cggacacaga agagtgtgcc aacggctatg gcagatgcgt tgttgttggg cattctcagt
     7141 tatttttttg gctttttatt gagaaggcaa ctcggccgtt tttggaagct ccgataatag
     7201 agtcggagca cccgcttgat aagctaccga atttgaacaa aactgtttcc aatttgataa
     7261 cagtcgggga gcctagtgca cgatatcaat gacatgctgt ttttcgccca cgtggtcaag
     7321 ttccgtagtt tttccgaggc agcacgccgg cttgatgttt ccaaatcgcg ggtgagtaag
     7381 gccgtcgccc gcctggaaag cgaactgggc gtccgcctgt tgcaccggag cacccgcagt
     7441 ctgagcttga ccgatgtcgg tgagtcatat ttcgagcatt gcgaccggat tttggaagag
     7501 ctgaaccagg ccgactcgac gatttcccgc ttgcatcagg agccgcgcgg caagctgaag
     7561 atcagcgccc cggtggcttt cagtacgatg catgtagcct cggccttgcc tgatttcatg
     7621 tcccgctatc ccgacctgac cgtcgatctg acgatcagcg accgcctggt cgaccttgca
     7681 gacgagggtt acgacatcgc cctgcgcatc acatgggagc ccgggcagaa tctggtggcc
     7741 cgccaattgg cacctatccg ccgcaaaatc tgcggcagcc cctcctacct ggcccgccaa
     7801 ggagttccac tcgctcccga ggatctcgtt aagcacaatt gcctcgacta caccttcatg
     7861 aacacccagg gtgtctggca tctcaatggc tgcaacggca acgtcgccat ccctgtctct
     7921 ggcacgctgc gtatcaacga tgacgaggcg ctgtcgcagg cggttctcgg cgggcttgga
     7981 ctggccgtat tgccgacgtt catcgttggc aaagatttgc aggcaggccg tctggtcgag
     8041 gttttgccag gctatgtgcc aactgaacgc ttcatctatg ccgttcatct gccgaatcgg
     8101 catttaccgt tgaaagtacg tgcttttatt gaatttcttc taaatcgttt tggaccaaca
     8161 ccctattggg atcgttgaga aaggaccctt tgaccaggta acagtgccga cctgccggtg
     8221 cttgccggaa ctgccgccga agaccacaat ccaacctcca aacacacgtc gccaccaccc
     8281 ccggcggcgt gctgtgccat cagcgcccac aaggcgtcat aaccggagag aaaaatgagc
     8341 aacatggctc tgccagccgc cacgctggac gacaaataca ccgccaccac cggccgcgtc
     8401 tttctcaccg ggacccaggc gctgatccgc ctgccgatgc tgcagcgcga acgcgatctt
     8461 gccgccggcc tgaataccgc cggtttcgtt tccggctatc gcggcagccc gctcggcaac
     8521 ctcgatcttg gcctgtggaa ggccaaggcg catctggccg aacaccacat cacgttccag
     8581 cccggcatca atgaagacat ggcggcgacg gccgtctggg gcagccagca ggttggcctc
     8641 tttcccggcg ccaaatacga tggcatcttt ggcctgtggt acggcaaggg gccgggcgtt
     8701 gatcgctgtg gcgacgtttt ccgccacgcc aatgccgccg gctcgtcgaa atacggcggc
     8761 gtgctggtga ttgccggcga cgaccatgcc gccaagtcat caaccctgcc ccaccagacc
     8821 gagcacattt tcaaggcgct gctgatgccg gtgctctacc cggccaatgt ccaggaatat
     8881 ctcgattacg gcctgcacgg ctgggcgatg agccgctatt ccggctgctg ggtcgccttc
     8941 aaggcgctgg ccgacacggt tgaaacatcc gcttcggtgc gcgtcgatct ggctgcggtc
     9001 aacatcgttt ttccgacgga tttcgcgctg ccgccggacg gcctcaacat tcgctggccc
     9061 gacccgccac tggtccagga agcccggctg ctcaacttca agctctacgc ggccctggcc
     9121 tatgcccggg ccaaccagct aaaccgggtg atcatcgact cgcccacccc ccggctcggc
     9181 atactgactg ccggcaagag ctatctcgat gtccgtcagg ccctggacga actcggcatc
     9241 gacgacgcgc tcgccgccga aatcggcatc cggctctaca aggtcggcat ggtctggccg
     9301 ctcgaaccgg aaggtgtccg ccacttcgcc gaagggctgg aagagatcct cgtcgtcgag
     9361 gaaaagcgg
//
LOCUS       DAORPM010000078         9320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1736_length_9320_cov_19.932974, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000078 DAORPM010000000
VERSION     DAORPM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1736_length_9320_cov_19.932974"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..466)
                     /locus_tag="PLD53_05410"
     CDS             complement(53..466)
                     /locus_tag="PLD53_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC60382.1"
                     /translation="MYLLDTNVISELRQGKPHPSPEVRAWAAGKPASQLFLSVISILE
                     LEKGVLALERRTPPQGSALRAWLTGVRAAFAGRILPFTDHAATLCAAMHVPNPHSERD
                     AMIAATALEHGMTVVTRNVADFAGTGVQLINPFAP"
     gene            complement(486..746)
                     /locus_tag="PLD53_05415"
     CDS             complement(486..746)
                     /locus_tag="PLD53_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015472933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRC60383.1"
                     /translation="MSVHTYSSRDFTRDVGAAKRATADGPVFITDRGRPAFALLKIED
                     YYRLAGQQETSLLEVMEAIPGGAGIEFDPPRLKVEFSTADLG"
     gene            complement(860..2137)
                     /locus_tag="PLD53_05420"
     CDS             complement(860..2137)
                     /locus_tag="PLD53_05420"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRC60384.1"
                     /translation="MSAIPAGYKQTEVGVIPEDWEVARLSSVSRTPVQNGVFNEPARK
                     GRGCKLINVGDLYTAAPIDINNLEKFDATKTEISKFGVTHGDLFFTRSSITPDGIAYC
                     NIYDQTTREDIVFDCHIIRVQADNKKVDPFFLAKFCVTRPARIYLVANAKTTTMTTVD
                     QAVIGGLPVPLPPLAEQRAIAAALSDVDALLAKVDQFIVKKRDIKQAVMQQLLTGKKR
                     LPGFSGEWEVKPMKSLGNTYGGLTGKTKIDFGHGRARYIPFMNVMTDTVIDAEWLEAV
                     DVASNEMQNLTQKGDLFFNGSSETPEEVGFCSVLLQDIPNLYLNSFCFGFRFNADAKI
                     NGLFFAYWFRSVKGRETMAVLAQGATRYNIAKSAFLRLELPQPTEAEQTAIATVLSDM
                     DAEIAALEQRRNKTRALKQGMMQELLTGRIRLV"
     gene            complement(2190..4589)
                     /locus_tag="PLD53_05425"
     CDS             complement(2190..4589)
                     /locus_tag="PLD53_05425"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HRC60385.1"
                     /translation="MALKKSELYSSLWKSCDELRGGMDASQYKDYVLILLFVKYVSDK
                     YAGDPDALIDVPAGGSFADMVAAKGSKEIGDKINKIIGKLADANDSLKGAINVADFND
                     EEKLGKADAMVQRLTKLVAIFEGLDFGGNRAEGDDLLGDAYEYLMRHFATESGKSKGQ
                     FYTPAEVSRIMSMVIDLGKASDVLQSIYDPTCGSGSLLLKAHDEAKNRTGLDLALYGQ
                     EMDNATSALARMNMVLHDCPTAQIWQDNTLSHPNFKNADGDLRTFDFVVANPPFSVKS
                     WTTGFDPANDLYHRFEYGIPPAKNGDYAFLLHMLRSLKRTGKAAVILPHGVLFRGGAE
                     AVIRKNLVRRGFIKGIIGLPANLFYGTGIPACILVLDKEGAAGRKGIFMVDASKGFIK
                     DGNKNRLRAQDIHKIVDTFTRLLETPKYSRWVPLADVAANDFNLNLPRYIDSTEPEDV
                     QDIEAHLKGGIPNADIDGLAAYWQVFPTMRRELFAEDARPGYSQPRIEASKVKTAIFA
                     HPEFTAFNQTVTTLFSEWKAANTPLLTGIQQGDRPKALIETFAESLLKTFRSGEAIAS
                     LIDPYGVYQHLMDYWDETMQDDAWMIVSDGWKALQAGKPNMDLIPPALIVARYFAAEQ
                     AVIEQREAERDAISRQMEEMDEEHGGEDGLLADAKNDKGKLTKASVKARQAEIKRDKE
                     AADERKLLDAYAELIEQEAAAGKKVKDAQKALDAKVAAKYAKLTEAEIKTLVVDDKWL
                     AALAASVQGELDRVSQALTGRIKQLAERYATPMPRMAGEVETLAARVDDHLKKMGFVW
                     Q"
     gene            complement(4910..5518)
                     /locus_tag="PLD53_05430"
     CDS             complement(4910..5518)
                     /locus_tag="PLD53_05430"
                     /inference="COORDINATES: protein motif:HMM:NF018691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC60386.1"
                     /translation="MAFLSQKGGSGKTTLAVHIAVAAVESGEQVVLVDTDIQQSATVW
                     SDARSESQPLVATASATELDRVLMAARHDAMTLCIIDTAPHATPDAVRIAQAVDLVVI
                     PCRPTAFDIAAVGSAVDIVRAAAVPAVLVLSACPFRAPEIAETRILLAEHGLPLAPVA
                     ITERRAFSRAIATGRAVTEFDPTGKAAEEIRALWHWLMEQMQ"
     gene            complement(5924..7051)
                     /locus_tag="PLD53_05435"
     CDS             complement(5924..7051)
                     /locus_tag="PLD53_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60387.1"
                     /translation="MYFPSARLKPKTVRSIEQQWRNQAMWIFLNNSFLSIVADPISDC
                     LLVRGRIKGDIERVFPDAEVVEGAGTDYRFRTFLPRVTVANALAYASSGMNYGNFKGS
                     VKERERHDEYMKVWRVMESWQSRAEAKERATGKAAAKMTEDEQRATDTGFPQAFLAQA
                     GAPERPPLANQEEMEGLLRQYLSQTADNYDAYLANELDSVTAQQNGLNIAQELTDVLM
                     GKKDAGYTLIPWFSPDQLGQYVRREIEFNGTPEESVFSLVVALFGKLYAIIRKAQAEF
                     RDIQDFLPELDFEITQYACIFLSLPIPAEPPTQEPKMPFTHCPTCDNPKGCARDQNCY
                     KEELAKPDPPYVEESPTVEKAYEAMNAVMDTIAARRKSKPS"
     gene            7894..>9320
                     /locus_tag="PLD53_05440"
     CDS             7894..>9320
                     /locus_tag="PLD53_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60388.1"
                     /translation="MSGRQLLPPSKSIILLTLISKLITERTARPATIAAIAIISCLGG
                     GLPAGAFAGITISISPEVFERSETRIYGLAKITATATLNPCSKPQIPNVLNCKLKDAG
                     DNTVFFADNIPFSGYIPKPNADGTQVVITPSTGAMLGLMASDLGYHSVGIELGDIYGN
                     KESATTTYLVVNNTLPSPTISASCVDDNLRLSLQSTVTATPEAKAHYYILYSSDIRIN
                     TFTDWRNFDFITSDSIKDITLESAKQGIWYQALVCSMLGYQEGKCSDFTDPVQSFCPV
                     APPLTSGSAVGVFRNGQWFLDANGNGAWDGCGTEFCFTGFGQAGDMPASGNWDGGSKS
                     YIGVLRSGTGEWFVDLNGNRKWDGCVADGCYVGFGAPGDLPVAGDWHGTGFAKIGVFR
                     NGQWFLDATGDGKWDGCSADLCLSFGQALDLPVAGNWNGGVPAGVGVFRAGTWYLDYN
                     GNGKWDGCQQDGGQDVCLYGSFGQAG"
BASE COUNT         1927 a         2571 c         2641 g         2181 t
ORIGIN      
        1 aacagggcca tcacgcgccg ctgggttttc ttttcaatct ggccgacgct ggtcatggcg
       61 cgaacggatt gatcagttgc accccagtgc cggcgaagtc ggccacattg cgcgtgacca
      121 cggtcatccc gtgttcgagg gctgtcgcgg cgatcatcgc gtcacgctcc gagtgcgggt
      181 tgggaacgtg catggcagcg cacagcgtcg cggcgtggtc ggtaaagggc aggatgcggc
      241 cagcgaaagc cgcccgtacc ccagtcagcc aggcgcgtag cgcactgccc tgcggcgggg
      301 tcctccgctc cagcgccagg acccctttct ccagttcaag gatgctgatt accgagagga
      361 aaagttgact cgctggcttg cccgctgccc acgcccggac ttcgggcgac ggatggggct
      421 taccttgacg taactcggag atcacgttgg tgtccagaag gtacattggc cgggcacccc
      481 agcgcttagc cgagatcggc ggtgctgaac tcgaccttca ggcgcggcgg gtcaaactcg
      541 atgcccgccc caccggggat ggcctccatg acctccagca gcgacgtttc ctgctgcccg
      601 gccaggcggt aatagtcttc aatcttgagc agcgcaaagg cgggccgccc acggtcggtg
      661 atgaacaccg gcccgtcggc ggtggctcgc ttggccgcgc ctacatcgcg ggtgaagtcc
      721 cggcttgaat aggtgtgaac ggacattttt ttctccttct ttgggctaac cctctgccag
      781 cgtccacttt catgtttgta tgttgtgaca ttgatggccg agtgtcaatg aacggtgttg
      841 accgttgact caggacactt cataccaacc gaatccgccc ggttagcagt tcctgcatca
      901 tgccttgctt gagggcgcgg gtcttgttgc ggcgttgctc tagggcagcg atttcagcgt
      961 ccatgtcgga gaggacggtg gcgatggcgg tttgttctgc ttcagtcggc tgcggcaact
     1021 caagcctcaa aaaagccgat ttcgctatgt tataccgagt cgcaccttgc gccagaacgg
     1081 ccatagtttc acgacccttt acgcttctaa accaataggc aaaaaataaa ccattgatct
     1141 tcgcgtccgc attgaagcga aaaccaaagc aaaagctgtt caagtaaaga ttcggaatgt
     1201 cttgcaggag gacggagcaa aagccaactt cctcgggcgt ttcggacgaa ccattaaaga
     1261 aaaggtcccc tttttgagtc aggttttgca tttcatttga cgcgacatcg acagcctcaa
     1321 gccactcggc gtcaatcacg gtgtcggtca tcacgttcat gaatgggatg tagcgtgccc
     1381 gcccatgccc gaagtcaatt tttgtctttc ctgtgagtcc gccgtaggta ttgccaagcg
     1441 atttcattgg ctttacttcc cactccccac taaaccccgg caatcgtttc ttgccggtaa
     1501 ggagctgctg cataacggcc tgtttaatat cgcgcttctt gacgatgaac tggtcgacct
     1561 tggcaagcag ggcatccaca tccgacagcg cggcggcgat ggcacgttgc tcggcgagag
     1621 gagggagtgg aactggcaaa cctccgatca cagcctgatc gacggtcgtc atcgttgtgg
     1681 tcttggcgtt agccacgaga tagattcgtg caggtcttgt tacacagaac ttagccaaga
     1741 aaaatggatc gaccttctta ttgtcagcct gaacccgaat aatgtggcag tcgaaaacaa
     1801 tgtcctctcg cgttgtttga tcgtagatat tgcagtaagc gattccgtct ggcgttattg
     1861 acgatcttgt gaaaaataag tccccatgag taacaccgaa cttggaaatt tccgtcttcg
     1921 tagcatcaaa tttctctaga ttgttgatgt caatcggcgc tgcagtgtac agatcaccta
     1981 cgtttattag cttgcagccg cgccctttcc tcgcgggttc gttgaatacc ccgttttgca
     2041 caggggtacg agagactgac gaaagtcgag cgacttccca atcctccggg atcactccca
     2101 cctcagtctg cttgtacccc gccggaatcg cactcatcgc agaacagttc tacagaaagt
     2161 cggcgctggc ggaacggcat tgaaatgcgt cattgccata cgaaccccat ctttttcagg
     2221 tggtcatcaa cccgggcggc cagcgtctcc acttctcccg ccattcgcgg catcggcgtg
     2281 gcgtagcgtt cagccagttg cttgatgcgc ccggtgaggg cttggctcac gcgatccagt
     2341 tcgccctgca cgctggctgc cagcgcggcc agccatttgt cgtccaccac caaggtcttg
     2401 atttcggcct cggtgagctt ggcgtacttt gcggccactt tggcgtcgag cgccttctgc
     2461 gcgtctttca cctttttgcc agcggcagcc tcctgttcga tcaattcggc ataggcatcg
     2521 agcagcttgc gctcgtcggc ggcttccttg tcgcgcttga tctccgcctg ccgcgctttg
     2581 acgctggcct tggtgagttt gcccttgtcg ttcttggcgt cagccagcaa tccatcctcc
     2641 ccgccgtgtt cctcgtccat ttcctccatc tggcggctaa tggcgtcgcg ttcggcttcc
     2701 cgttgctcga tgacggcctg ttcagccgcg aaatagcgag caacgatcaa cgcgggcggg
     2761 atcaggtcca tgttcggctt gccggcctgt agggctttcc agccgtcact gacgatcatc
     2821 caggcgtcgt cctgcatagt ctcgtcccag tagtccatca gatgctgata gacgccgtag
     2881 gggtcgatta gtgaagcgat ggcttcccca ctgcggaagg ttttcagcag gctttccgcg
     2941 aaggtttcga tcagcgcctt gggacgatca ccttgttgga taccagtgag cagcggggtg
     3001 ttggcggctt tccattcgct gaacagcgtc gtcacggtct ggttgaaggc ggtgaattcg
     3061 gggtgagcga agatggcggt cttaactttg ctggcttcga tccggggctg gctgtagccg
     3121 gggcgggcat cctcggcaaa cagttcgcgg cgcatggtgg ggaaaacttg ccagtaggcg
     3181 gccaggccgt cgatgtcggc gttggggatg ccgcccttga ggtgcgcctc aatatcttgt
     3241 acgtcttccg gttcggtgct gtcgatgtag cgcggcagat tcaggttgaa gtcgttagcc
     3301 gcgacatcgg ccagcggcac ccagcgcgaa tacttgggag tttccaacag acgggtgaag
     3361 gtatcgacga tcttgtggat gtcctgcgcc cgcagccggt tcttgttgcc gtccttgatg
     3421 aagcccttgc tggcatcgac catgaagatg cccttgcgcc ccgctgcgcc ttccttgtcc
     3481 agcaccagaa tgcaggcggg gatgccggtg ccgtagaaca ggttggccgg caaaccgatg
     3541 atgcctttga tgaagccgcg ccggacaagg ttcttgcgaa tcacggcctc ggccccgccc
     3601 cggaacagca cgccgtgcgg aagaatcacc gccgccttgc cggtcctctt gaggctcctg
     3661 agcatgtgca gcaggaaggc gtagtcacca ttcttggccg gggggatgcc gtactcaaag
     3721 cggtggtaca ggtcattggc gggatcaaaa ccggtcgtcc atgacttgac tgaaaacgga
     3781 ggattagcca ccacgaagtc gaaggttctg aggtcgccgt cggcattctt gaagttcggg
     3841 tgtgacaggg tattgtcctg ccagatttgc gccgtcgggc agtcgtgcag caccatgttc
     3901 atccgcgcca gcgccgaggt ggcgttgtcc atctcctgcc cgtacagcgc caaatccaac
     3961 ccggtgcgat tcttggcttc gtcgtgggct ttgagcagca gtgaacccga cccgcaagtg
     4021 gggtcgtaga tgctctgcaa gacatcgctg gctttgccca gatcgatgac catcgacatg
     4081 atgcgcgaga cttccgccgg ggtgtagaac tggcccttgc tcttgcccga ttcggtggcg
     4141 aagtggcgca tcaggtattc gtaggcatcg ccgagcaggt cgtcgccttc cgcacgattg
     4201 ccgccgaaat ccagcccctc aaagatggcg accagcttgg tgagccgttg caccatcgca
     4261 tcggccttgc cgagcttttc ctcgtcgttg aagtcggcga cgttgatcgc gcccttgagg
     4321 ctgtcgttag cgtcggcgag cttgccgatg atcttgttga tcttgtcgcc aatctccttg
     4381 ctgcccttgg cggccaccat gtcggcaaag ctgcctcccg caggcacgtc gatcagggca
     4441 tcgggatcac ccgcgtactt gtcggacaca tatttgacga acagcaggat gaggacgtaa
     4501 tccttgtact ggctggcatc catgcccccg cgcagttcat cgcaggattt ccagagagag
     4561 gaataaagtt cggatttctt gagagccatg agtcgagttt gagcgtgggg cagctacgag
     4621 aatgaagcca taacgtcata gcgtcatgac tttaatgcag gcaaaccttt gtccgtgaaa
     4681 atgcgagaga gtccacgcac cgcgagctgt tggatactgg ttccttcggc cacagccagt
     4741 tgatgcagcc gttcccaatc ggcacggggg agacgcaccg ttaacgcgac cacttcgcct
     4801 tttccccgct gtcggactgt agcggcggca tcaatcgggg tggaggatac atctcccaaa
     4861 ggaggcgttt tacgggtgaa ttccgccaag ccggtactgt taggttttct cattgcattt
     4921 gctccatgag ccagtgccac aaggcgcgga tttcttcagc cgccttgccg gtgggatcaa
     4981 actcggtcac cgctcgcccg gtcgcgatgg cccgtgaaaa ggcgcggcgt tcggtaatcg
     5041 ccaccggagc cagcggcaag ccatgttcgg ccagcaatat ccgcgtctca gcgatctctg
     5101 gggcgcgaaa cggacacgcc gataacacca gcactgcggg caccgcagcg gcccgaacaa
     5161 tgtccaccgc gctaccgacc gccgctatat cgaacgcggt tgggcggcaa ggaatgacca
     5221 ccagatcgac ggcctgagca atccgaacgg catcaggagt tgcatgggga gcggtatcga
     5281 tgatgcacaa cgtcatagcg tcatgacgtg ccgccatcaa aacacggtct aattccgtcg
     5341 ctgaagccgt cgcaaccaag ggctgagatt cggatcgcgc atcgctccac actgtggcgc
     5401 tctgttggat gtcggtatcc accagcacga cctgttcgcc ggactccacc gctgctactg
     5461 cgatatggac agcaagggtc gtcttgccac tgccgccctt ctgactgagg aacgccattg
     5521 atttcataac gccatgaaat cataacgcct tgacgctatc aagtgctcag tgattcggga
     5581 cattctaaga acagtgatcc ctctacgaat gatctgaata ctcgtcaaaa ttacattcat
     5641 tcttgttcct tcaatttcac cctatctgat cgatttcacc tatcgcccga aaaaatggtc
     5701 agcgaagtcg gagaggtata gggggatcgg cagggcgcgg aatttagggg tgggggggat
     5761 caaggccggg aggccagccc tgaaaacagg gccgtcgatg gcccgtaccg ggggactttt
     5821 ctttttctat aagaccttac gcaacgaacg aaacagggtg atggactcgt tttacctttt
     5881 cgaggggtta ggcaaaaaca gccatgctcg gtttccggct cactcacgac ggcttgctct
     5941 tacgtcgcgc tgcaatggtg tccatcacgg cgttcatcgc ctcgtaggct ttttcaacag
     6001 tcggcgattc ctcgacatag ggcggatcgg gctttgccag ttcttcctta tagcaattct
     6061 ggtctctggc gcagcctttg gggttgtcac aggtcgggca gtgcgtaaac ggcatcttgg
     6121 gttcctgagt gggaggttcg gcgggaatcg ggagcgacag gaaaatgcag gcgtactgcg
     6181 taatctcaaa atccagttcc ggcaggaagt cttgaatatc gcggaactcg gcttgcgcct
     6241 tgcggatgat ggcgtacaac ttaccgaaca gcgcgacgac gagcgagaat acggattcct
     6301 ctggagtgcc gttaaactcg atctcgcgtc gcacatactg gcctaattga tccggcgaga
     6361 accagggaat cagggtgtag cccgcgtcct ttttccccat cagaacatcg gtcagctcct
     6421 gggcaatgtt cagcccgttc tgctgtgcgg tgacgctatc cagttcgttc gccagatagg
     6481 cgtcgtagtt gtccgccgtt tggctcaggt actggcgcag cagcccttcc atttcctcct
     6541 gattcgccag cggcgggcgc tccggcgcac cggcttgagc cagaaacgct tgcgggaagc
     6601 cggtatcagt ggctctttgt tcgtcttcag tcatttttgc agcggctttt ccagtcgctc
     6661 gctctttagc ttcagccctt gactgccacg actccataac ccgccaaact ttcatgtact
     6721 cgtcatgacg ctcgcgctct ttaacgctgc ctttgaaatt gccgtagttc ataccgcttg
     6781 atgcataggc cagcgcattg gcgaccgtca ccctgggcag gaaggtacga aaccggtagt
     6841 cggtgcccgc gccctccacg acctcagcgt cgggaaacac ccgttcgatg tcgcccttaa
     6901 ttcgcccgcg caccagcagg cagtcgctga tagggtcggc cacaatactc aggaaagaat
     6961 tattcaagaa aatccacatc gcttgatttc gccattgttg ttcaatactt cggacagttt
     7021 ttggtttcag cctcgcggac ggaaaataca tcggtgcatg aaaccccttg aaaaatcaga
     7081 gcttcaagca tccgtcgaat ttcaagcaaa atcaacgatt tattttatcc aactcaggca
     7141 aacccctgac tttgatttaa ttcagcctat gaaggctaac ctgttgatgg tgatcaccaa
     7201 aactgtccga agtattatta ttcaataggt taaatagaat cgttggattt ttcgccaact
     7261 cctaagcaaa tcccatgctg tgccgcaaca atcggggcaa ctgttacgaa gggacccccg
     7321 cgtaacggtt gccccgattg ttgcaacaca gcatggcgct ggcgaattct gtggggttga
     7381 tgctgcggat tctggaagca ggcagtttga ttacagcgcg ggcgtaagag taagcgggag
     7441 ttggggcgga tcaagggtgg ttcaatcatc cgaatgatcg gggctggtgc tggcggattc
     7501 tgtgggtgct ggtgctggtg aagtgggaag ctggcgggcg ggcaggtcgc cgcgaaaata
     7561 aattcagccg gcgtgatctt gggtagccgg ggggcgtttt ccgcgcccgg atgatctgag
     7621 tagacctagc cgaaaattcg cggcaaattt tcttgaagca atctcgattg ccgcccgcat
     7681 tgtccggtct gtctgtaata ggtagttttt cccactcagg aaacgcccgc agatttcaaa
     7741 aggatgctgt acttgggtag gcgtaaggat tccttcgtga atgaataaca agcacggtca
     7801 cccctgctag acattgcgtc taaatgtcaa tagtgtggtg agtgtctaaa atttagacca
     7861 caaccatcag ccctcccggt aaaaggaatc tccatgtcag gccgtcagtt attacctccc
     7921 tccaaatcaa ttattttatt aactctaatt agtaagttaa taacagaaag aaccgctaga
     7981 ccggcaacta ttgcggccat agccattatc agttgcttag gaggaggact gccagcgggg
     8041 gcatttgcag gcatcactat tagcatttcg cctgaagttt ttgaaagatc agagactagg
     8101 atatatggtc ttgccaagat tacagctacg gcaaccttaa atccatgttc taagccgcaa
     8161 attccgaatg tactcaactg taagctaaaa gacgccgggg acaatacagt cttctttgct
     8221 gataatatcc catttagtgg ctatatacct aaacctaacg ccgatggaac acaggttgtc
     8281 attacgccat caactggagc aatgcttggg ttgatggcct ctgacctagg ttatcacagt
     8341 gtaggtattg aactcgggga tatctatggc aataaggagt cggctactac cacttatcta
     8401 gtagtgaata atacattgcc aagtcccact atttcggcaa gctgcgttga tgataatttg
     8461 agattgtctc ttcaatccac agttacagca actcctgagg cgaaggcgca ctattatatt
     8521 ttgtatagtt cggatattag aatcaataca ttcactgatt ggagaaattt tgattttata
     8581 acctcggata gcataaaaga catcacgctt gagtcagcca aacaaggcat atggtaccag
     8641 gctctggtgt gtagtatgtt gggatatcaa gagggtaaat gcagtgattt cacggatcca
     8701 gtacaaagct tttgtcctgt agcaccaccg ctcacttccg gctctgccgt cggcgtgttc
     8761 cgcaacggtc aatggttcct cgacgccaac ggcaacggcg cgtgggacgg ctgcggcacc
     8821 gagttctgct ttaccggctt tggtcaagcg ggcgatatgc ccgcctctgg caactgggac
     8881 ggtggcagca agagctacat cggggtgttg cgttccggca ccggcgaatg gttcgtagac
     8941 ctcaacggca accggaaatg ggacggctgc gtcgctgacg gctgttatgt cggctttggt
     9001 gcgcccggcg acttacccgt cgcgggcgat tggcacggca ccggcttcgc caagatcggc
     9061 gtgttccgca acggccagtg gtttctggat gccactggcg acgggaaatg ggacggctgt
     9121 agcgccgatt tgtgcctgag cttcggccaa gcccttgatc taccggtggc gggtaactgg
     9181 aatggcggcg ttccggcggg ggtgggcgtg ttccgcgccg gcacttggta tctcgactac
     9241 aacggcaacg gaaaatggga cggctgtcag caggacggcg ggcaggatgt ctgcctctac
     9301 ggcagcttcg gccaagccgg
//
LOCUS       DAORPM010000079         9223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1769_length_9223_cov_15.801156, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000079 DAORPM010000000
VERSION     DAORPM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1769_length_9223_cov_15.801156"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2954
                     /locus_tag="PLD53_05445"
     CDS             <1..2954
                     /locus_tag="PLD53_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRC60389.1"
                     /translation="EDQVTYPLTTAMLSVPKSKVVRGFSFFGASFVYIIFEDGTDIYW
                     ARSRVLEYLNFASSRMPKGVTPSLGPDATGVGWVYQYALLARDKTLAELRTIQDWYVR
                     YQLTKAHGVAEVASVGGFVQTYQVTVDPVKLRSYGIPLMKVAQAIRDSNRDVGGRVVE
                     MAETEYMVRGKGYLRGRSDIEMLVVKSDRGTPVLIRDIARVELTPDERRGLTELNGEG
                     EVVSGIAMARYGANALEVIQNLKTKIAEIASGLPEGVSVEAVYDRSELIHRAIDTLKR
                     TLIEEAIIVALVCIVFLMHVRSALVAILMLPVGVLISFIAMRSLGMNSNLMSLGGIAI
                     AIGAMIDAAIVMIENAHKHIERLPDEHSDSDRIDAMLSACREVGPALFFSLLIITVSF
                     LPVFALEGQEGRLFSPLAYTKTFAMAGAALLSVTLVPVLMILFIRGKIMPESRNPVNR
                     SLIWAYRPIITGVMRWKKATIVAAVAVLGISYYPASQLGSEFMPTLNEGSLLYMPTSL
                     PGMSITKAAELLQTQDKIIKSFPEVASVFGKAGRVNSATDPAPTEMFETVINLKPDSE
                     WRPGMTTDKLIAELDKALQFPGVANSWTMPIKARIDMLSTGIRTPIGIKVFGKDLGEM
                     EKLAKQIEAVVKTVPGTTSAFAERITGGFYLNIEPDREQLARYGLAVGDLQDVIGSAL
                     GGEMVTTTVEGRERFGVSVRYPRELRSDPQQIAREVLIPTMDGAMIPLGQLAKVAVAK
                     GAPSIRTENALLSAYIYVDIRDRDLGGYVADAKKAVAEQVEFPPGYYVTWSGQFEYME
                     RAIAKMKIVIPVTLLSIFLLLYLNFRRLTETLIVMLSVPFALVGGVWLMWLLDYNLSV
                     AVAVGFIALAGVAAETGVVMLIYLDQAWAEAKAACHLSGRKPTTSDLYAAVMEGAVER
                     VRPKMMTVVAIMAGLLPIMWGTGTGSEVMSRIAAPMVGGMISSTVLTLAVIPAIYALV
                     KQWRLKRGYEL"
     gene            complement(2951..4246)
                     /locus_tag="PLD53_05450"
     CDS             complement(2951..4246)
                     /locus_tag="PLD53_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC60390.1"
                     /translation="MPELDRAQRRRRNTALVVVLAAYVISFFQRFAPAGIAQDLTAAF
                     NTTASSLGALAATYFYVYTVMQVPTGIFVDTFGPRRILLLGGIVGAAGSLLFGMASSL
                     ELALVGRTLIGLGVSVTFISMLKFIAVWFDEKRFASMVGVGLLVGNLGSVLAGAPLSW
                     LAQAVGWRSVFVAIACVSLLLGIACWVLVRDRHDGGTIGEVKKPHFDRTVVLSGLLQV
                     VRNRATWPAAWINLGVSGSFFAFGGLWATPFLVEAHGMTRTVAASHVSLYFTCFALSC
                     VGVGTLSDRLGKRKPVLVVGTHLYALTWVVWLSGVALPLYASYGMFALMGILTASFTL
                     TWACAKEVNPPLLSGMSTSITNMGGFLAGAILQPLFGWAMDLHWAGGVSSSGARQYTA
                     GDYHAGIVLLALTAAIGAIASWRIRETNCRNIWLSSSEN"
     gene            complement(4361..5101)
                     /gene="rlmB"
                     /locus_tag="PLD53_05455"
     CDS             complement(4361..5101)
                     /gene="rlmB"
                     /locus_tag="PLD53_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRC60391.1"
                     /translation="MSQTRFIHGFHAIIAKLRHQPDAILEIFIDAERHDARVRDLMRH
                     AELHTVRVVPVDAKRLEGMAGGDMRHQGVVARVSGESRHVALDDVLDTLEEPAFLLVL
                     DGIQDPHNLGACLRVADAVGAHAVIAPKDRAVGLTQTAIKVASGAAESVPYITVTNLA
                     RTLRELKEREIFLIGTDADATEDLYSAQWPLATAWVLGAEGDGMRRLTREACDQLVSI
                     PMLGSVQSLNVSVATGVCLYEARRRRGN"
     gene            complement(5201..7432)
                     /gene="rnr"
                     /locus_tag="PLD53_05460"
     CDS             complement(5201..7432)
                     /gene="rnr"
                     /locus_tag="PLD53_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRC60392.1"
                     /translation="MSIKKLSKTRTNDPHFDREVAKYEHPLPSREYVLQVLEEQGKPV
                     SFDELSALLDIHQQESEMFQRRLAAMERNAQLMRNRKGSYIVPERASLIAGRVEGHAD
                     GYGFFIPDEGGDDLFLDAKQMSRVLHRDRALVRVVGMDRRGRREGVIVEVLERANSRV
                     VGRVLVEHGITVVVPENRRINQDILVAPEKRSKARAGQVVIVEIIEQPNRHAQPIGRI
                     VEVLGNYADPGMEIEIALRKHDMPFEFSPKVLEETKALPAKVRKTEWAGRKDLRDLAL
                     VTIDGETARDFDDAVFAEKKGRGWRLVVAIADVSHYVRPGMALDREAMDRGNSVYFPR
                     RVIPMLPEKLSNGLCSLNPDVERLAMVCDMDINSRGEIKDYTFYPAVFLSRARLTYDQ
                     VWSWLSGIAEPQNDTHRAVQPQLQALYALFHILLKARHKRGAIDFETIETMMLFNDQG
                     KIDNIVPVFRNDAHRLIEECMLAANVCASAFLEEHKQPCLYRVHEGPTPEKLEGLRNF
                     LKEFGLSLPGGESPTAKDYGALLEKIKPRPDAQLLQTVMLRSLRQAMYSPDNVGHFGL
                     SYEHYTHFTSPIRRYPDLLVHRSIKAVLDGVRYSPGKWDDIGLHCSMTERRADEASRD
                     VNNWLKCYYMRDRIGEEFAGTIASVVPFGVFVALDSVYVEGLVHVSELGEDYFKYDNV
                     KHLMLGERSGQRFRLGDRLQVKLVRADLETGRIDFVLANPSAQTHVPALKPASKGASK
                     ARR"
     gene            7472..7558
                     /locus_tag="PLD53_05465"
     tRNA            7472..7558
                     /locus_tag="PLD53_05465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7506..7508,aa:Leu,seq:cag)
     gene            7755..8867
                     /locus_tag="PLD53_05470"
     CDS             7755..8867
                     /locus_tag="PLD53_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRC60393.1"
                     /translation="MEKIAAIVIGAGVVGLACARALAQRGIATIILERHDAFGTETSA
                     RNSEVIHAGLYYPSGSLKAQLCVAGRRLLYDYCATHGVRHRRCGKLIVATSSEQEARL
                     DALRRQGETNGVDDLQPLGAGDARRLEPELRCTAALLSPSTGIIDSHGLMLALLGDAE
                     RNGATLAVCSPVCAGRIEDDGMILEIGGKDPTQLKADIVINAAGLYATQVAASLEGFP
                     AEQIPPAFYAKGNYCALTAKSPFTRLIYPLPEAGGLGIHLTLDLGGQARFGPDVEWLP
                     ALGDKPDYRVDPVRADAFCNEIRRYWPGLHDGALTPAYAGIRPKISGPQDAAADFLIQ
                     GQESHGVPHLINLFGIESPGLTASLAIAEHVVERLG"
     gene            complement(8883..>9223)
                     /locus_tag="PLD53_05475"
     CDS             complement(8883..>9223)
                     /locus_tag="PLD53_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC60394.1"
                     /translation="IARAFALSPKLLLLDEPFGMLDSLTRWDLQEVLMEVWSRSQLTA
                     MMVTHDVDEAILLADRVVMMTNGPRAKVGKVLEINLPRPRTRKMLLEHPDYYPLREEL
                     LGFLSDCGHG"
BASE COUNT         1908 a         2858 c         2670 g         1787 t
ORIGIN      
        1 tcgaggatca ggtaacctat ccgctcacca cggccatgtt gtcggtgccg aaatccaagg
       61 tcgtgcgtgg cttctcattc ttcggcgcgt ccttcgttta tatcattttc gaggacggca
      121 ccgatatcta ctgggctcgc tcgcgcgtgc tcgaatatct gaatttcgcc tccagccgca
      181 tgccgaaggg cgtcacgcca tcgctcgggc cggatgccac cggagtcggc tgggtttacc
      241 agtatgcgct tctggccagg gacaagacgc tggccgaact acgcaccatc caggactggt
      301 acgtccgcta tcaactgacc aaggcgcacg gtgtcgccga agtcgcttca gtcggcggct
      361 tcgtgcagac ctatcaggtc accgtcgacc cggtgaagct gcgttcctac ggtatcccgc
      421 tgatgaaggt ggcgcaggcg atccgcgact cgaaccgcga tgtcggcggc cgcgtcgtcg
      481 agatggccga aaccgagtac atggtgcgcg gcaagggcta cctgcgcggc aggagcgaca
      541 tcgaaatgct ggtcgtcaag agcgacaggg gcacgccggt tctgatccgc gacatcgccc
      601 gggtcgaact gactccggac gaacggcgcg gcctgaccga actcaacggc gaaggggagg
      661 tggtgtcggg cattgccatg gcgcgctacg gcgcgaacgc gctggaagtg atccagaacc
      721 tcaaaacaaa gattgctgaa atcgcctccg gcctgcccga gggcgtaagc gtggaagcgg
      781 tatatgaccg ttccgagctg attcatcgcg ccatcgacac gctcaagaga acgctgatcg
      841 aagaagcaat catcgtcgcg ctggtgtgca tcgtcttcct gatgcacgtg cgcagcgcgc
      901 tggttgcgat cctgatgctt ccggtcggcg tactcatctc ctttatcgcc atgcgttcgc
      961 tgggaatgaa ctccaacctg atgagtctcg gcggcattgc cattgccatc ggcgccatga
     1021 tcgacgcggc catcgtgatg atcgagaacg cccacaagca catcgaacgc ctgcctgacg
     1081 aacactcgga cagtgatcgc atcgatgcca tgctttctgc gtgcagagag gtcggaccgg
     1141 cgctgttctt ttcgcttctg atcatcaccg tatccttcct gccggtattc gcgctggagg
     1201 ggcaggaagg gcggctgttc tcaccgctgg cctacaccaa gacctttgcc atggccggcg
     1261 ccgccctgct ctcggtaacc ctggttccgg tgctgatgat cctgttcatc cgcggcaaga
     1321 tcatgccgga gtccaggaat ccggtgaatc gctccctgat ctgggcctat cggccgatca
     1381 tcaccggcgt catgcgctgg aagaaggcga ccatcgtcgc ggccgtggcg gttctcggca
     1441 tctcgtacta tcctgcgtcg cagctcggct ccgagttcat gccgacgctg aacgaaggat
     1501 cgctgctcta tatgcctact tcgctaccgg gaatgtcgat caccaaggca gcggaactcc
     1561 tgcaaactca agacaagatc atcaagagct ttcccgaagt ggcctcggtc ttcggcaagg
     1621 ccgggcgcgt aaactcggcg accgatccgg caccgaccga aatgttcgag acggtgatca
     1681 acctcaagcc cgactccgaa tggcgccccg gaatgaccac cgacaagctg attgccgaac
     1741 tcgacaaggc tctgcaattc ccgggggtgg caaactcatg gacgatgccg atcaaggcgc
     1801 gaatcgatat gctgtcgacc ggcatccgta cgccaatcgg catcaaggtc ttcggcaagg
     1861 atctcggcga aatggaaaag ctggccaagc aaatcgaggc cgttgtgaag acagtacccg
     1921 gcaccacaag tgcttttgcc gaacgaatca ccggcggctt ctatctcaac atcgagccgg
     1981 atcgagaaca actcgcgcgt tacgggctcg cggtcggcga cctgcaggac gtgatcggca
     2041 gtgcgctcgg cggtgaaatg gtgaccacca cggtcgaagg ccgcgaacgt ttcggcgtct
     2101 ccgttcgcta tccgcgcgag ctgcgctccg acccgcagca gatcgcccgt gaagtactga
     2161 taccgacgat ggacggcgcg atgattccac tcgggcaact ggccaaggtg gccgtcgcca
     2221 agggggcgcc cagcatccgc accgagaacg ccctgctctc cgcgtacatc tatgtcgaca
     2281 ttcgcgaccg cgacctcggc ggctacgtcg ccgacgcgaa aaaggcggtc gccgaacagg
     2341 tcgaattccc gcccggctac tacgtcacct ggagcggcca gttcgagtac atggaacggg
     2401 caatcgcgaa gatgaagatc gttattccag tgacattgct ttccatattc ctgttgctct
     2461 accttaactt cagacgcctc accgaaacgc ttatcgtcat gctctcggtg cccttcgcct
     2521 tggtcggcgg cgtctggctg atgtggttgc tcgactacaa tctgtcggtg gcggtggcgg
     2581 tcggatttat cgccctggcc ggtgtcgcgg ccgagaccgg cgtggtcatg ctgatctacc
     2641 ttgatcaggc ctgggcggag gccaaagcgg catgtcacct gtccggacgc aaaccgacga
     2701 ccagcgacct gtatgcagcc gtaatggaag gcgccgtcga gcgtgtccgc ccgaaaatga
     2761 tgacggtcgt ggccatcatg gccggcctgc tgccgatcat gtggggcacc ggtaccggct
     2821 cggaggtaat gagccgcatc gccgcgccga tggttggtgg aatgatatct tcaacggtgc
     2881 tcacgctcgc cgtcattccg gccatctacg cactggtcaa gcagtggcgt ctgaagcggg
     2941 ggtatgaact ctagttttca ctcgacgaca gccagatgtt ccggcaattg gtttcgcgaa
     3001 tgcgccagga agcgatggcc ccaatcgccg ccgtcaacgc cagcagaaca atgccggcat
     3061 gataatctcc cgcggtgtat tggcgcgcgc ctgaactgct cacgccaccg gcccaatgca
     3121 gatccattgc ccagccgaat agtggctgca aaatcgctcc ggcgagaaag ccgcccatat
     3181 tcgtaatact ggttgacatg ccggaaagca gcggcggatt gacttccttg gcacatgccc
     3241 aggtgagcgt gaagctggcg gtaagaattc ccatcagcgc aaacatgccg tagctggcgt
     3301 acaggggaag cgcaacgccg gagagccaga cgacccaggt caacgcgtag agatgggtgc
     3361 caacgacgag taccggcttg cgtttgccaa ggcgatccga aagcgtaccg acaccgacgc
     3421 acgacagcgc aaaacaggtg aaatacaagc tgacgtgact ggccgcaacg gtacgcgtca
     3481 tcccgtgcgc ctcaaccaga aacggcgtcg cccacaagcc accaaacgca aagaagcttc
     3541 cgctgacgcc aagattgatc cacgcagctg gccaggtcgc acggtttctg acaacctgta
     3601 gcaggccgga gagaactacc gtgcggtcaa agtgcggctt ctttacttca ccaattgtgc
     3661 cgccatcgtg tcgatcgcgc acaagcaccc aacaggcgat accgagcaac aacgaaacgc
     3721 acgcgatagc gacaaagaca gatcgccatc caaccgcttg tgccaaccat gacaatggag
     3781 cgcccgccag caccgagcca agattgccga ccagcaaacc cacgccgacc atgctcgcaa
     3841 accgcttttc gtcaaaccac acggcgatga acttgagcat ggaaataaag gtaacggaga
     3901 cgcccaggcc gatcagggta cgtccgacca gcgccaactc cagcgagctc gccataccga
     3961 acaagagact ccccgcagca ccaacaatac cgcccagcag gagaatccgc cttggcccga
     4021 aggtgtcgac aaaaatgccg gtgggcactt gcattaccgt gtaaacgtag aaataggtcg
     4081 ccgccagcgc accgagcgat gaggccgtgg tattgaaggc cgcagtcaga tcctgggcga
     4141 tgcccgccgg tgcaaaacgc tgaaaaaacg aaatgacata ggccgccagg acgacgacca
     4201 aagccgtatt gcgacgacgg cgctgcgccc ggtcaagctc aggcatggaa cttccttcaa
     4261 tcaggttgaa taatccgtta tcgcccggaa ctcggtgcaa gcccaccagg gtgtgaaaac
     4321 atcagaatta tcacgagcaa gctttggaga tatttgacaa tcagtttccg cgtcgccgcc
     4381 ttgcctcgta caggcaaact ccggtagcga ccgatacgtt caggctctgc accgaaccga
     4441 gcatcggaat actcaccagt tgatcgcagg cttcgcgcgt caggcggcgc atgccatcgc
     4501 cttcggcgcc gagcacccac gcggtggcca gcggccattg tgccgagtag aggtcttccg
     4561 tggcgtcggc atcggtaccg atcaggaaga tttcacgttc tttcagctcg cgtagcgtac
     4621 gcgccagatt ggtcacggtg atgtagggca cgctctccgc cgcaccgctg gccaccttga
     4681 tcgccgtttg tgtcagaccg actgccctgt ctttcggggc gatgacggcg tgcgcgccca
     4741 cggcgtcggc aacacgcagg caggcaccga gattgtgcgg gtcctgaatg ccgtccagca
     4801 ccagcaggaa agcgggttct tccagcgtat ccagcacatc gtcaagcgcg acatgacgcg
     4861 actcgccgga aaccctggcc accacccctt gatgccgcat atcgccaccg gccatgcctt
     4921 cgagtcgttt tgcatccacc gggacgaccc tgactgtgtg cagctcggca tggcgcatca
     4981 gatcccgcac cctggcatca tggcgttcag cgtcgatgaa tatctcaaga atggcgtcgg
     5041 gttgatgacg cagtttggcg atgatggcgt gaaatccgtg gatgaagcgg gtttgtgaca
     5101 tgaatgattc tcgcaggcgg aattagaagc gtattttact ttggcacggg aaacgtccgg
     5161 gcggccaagc ccttgacgtg aggacagcgt aagcgcgcac tcagcggcgc gcttttgacg
     5221 cgcctttcga agctggcttc agtgcaggca cgtgggtttg cgccgatgga ttggccagca
     5281 cgaagtcgat acgtccggtt tccagatcgg cgcgcaccag tttgacttgc agacgatccc
     5341 ccaaacgaaa gcgttgtcca cttcgctcac cgagcatcaa atgcttgacg ttgtcgtact
     5401 tgaaataatc ctcgcccaac tcggaaacgt ggaccaggcc ttcgacatag acggaatcca
     5461 gcgcgacgaa aacgccaaat ggaacgaccg acgcaatggt tccggcaaat tcctcgccga
     5521 tccggtcgcg catgtaatag cacttgagcc agttgttcac gtcacgcgat gcctcgtcgg
     5581 cgcgccgctc ggtcatcgag caatgcaggc cgatatcgtc ccacttgccc ggcgaatacc
     5641 gcacaccatc gagcacggcc ttgatcgagc ggtgcaccag caaatcgggg taacgccgga
     5701 tcggcgacgt gaagtgcgtg tagtgctcgt aggaaagacc aaagtggccg acattgtccg
     5761 ggctgtacat ggcctgtcgc agcgagcgca acatcacggt ttgcagtagt tgggcatccg
     5821 gacgcggttt gatcttttcg agcagcgcgc cgtaatcctt ggccgtcggg ctttcgccac
     5881 cgggcaggct caggccgaat tccttgagaa agttgcgcag accttcgagc ttctccggcg
     5941 tcggcccttc gtgcacccga tacagacacg gctgcttgtg ctcttcgaga aacgccgagg
     6001 cgcacacatt ggccgccagc atgcattcct cgatcaggcg gtgggcatcg ttgcggaata
     6061 ccggaacgat gttgtcgatc tttccctggt cgttgaacag catcatcgtc tcgatcgttt
     6121 cgaaatcgat cgcaccacgt ttatgccgcg ccttcaacag gatatggaac agcgcgtaca
     6181 gcgcctgcaa ttgcggctgc acggcgcgat gcgtatcgtt ctgcggctct gcgataccgg
     6241 atagccagct ccacacctga tcgtaggtca gccgcgccct ggaaaggaag acggctggat
     6301 aaaacgtata gtccttgatt tcgccgcggc tgttgatatc catgtcgcac accatggcca
     6361 ggcgctcaac gtcaggattc aacgaacata gaccgttgga gagtttttcc ggcagcatcg
     6421 ggatcacccg acgcgggaaa tagacggaat tcccgcgatc cattgcctcg cgatcaagtg
     6481 ccatgccggg tcgaacgtag tggctgacgt cggcaatggc caccaccagc cgccagccac
     6541 gccccttctt ctccgcaaag acggcgtcgt cgaaatccct tgccgtttcg ccgtcgatag
     6601 tcacgagcgc tagatcgcgc aaatccttgc gtccggccca ctcggttttc cggaccttgg
     6661 ccggcaatgc cttggtctcc tccagaacct ttggcgaaaa ctcgaaaggc atatcgtgct
     6721 tgcgcagcgc gatctctatt tccatgccgg gatcggcgta attgcccagc acttcgacaa
     6781 tgcgcccgat gggctgcgcg tgacggttag gctgttcaat gatttcgacg atcaccacct
     6841 ggcctgccct ggccttggat ctcttttccg gcgcaaccag aatgtcctgg ttgatacggc
     6901 ggttttcagg tacgactacg gtaatgccat gctcaaccag aacccggccg acgacccggc
     6961 tgttggcccg ttctagcacc tcgacaatta ccccttcgcg acgcccccgg cgatccatgc
     7021 cgacaacccg taccagcgcc cgatcacgat gcagcaccct ggacatctgt ttggcatcaa
     7081 gaaacagatc gtctccaccc tcgtcgggga tgaaaaagcc atagccgtcg gcatgccctt
     7141 ccacgcgccc ggcaatcagg ctggcgcgct ccggaacgat gtacgagccc ttgcggttgc
     7201 gcatcagctg cgcgttgcgc tccatcgcag caaggcggcg ctggaacatc tcgctttcct
     7261 gctgatgaat gtcgagcagc gcgctcagct cgtcgaaact gaccggtttg ccctgctcct
     7321 ccaggacttg caacacatac tctcgtgacg gcagggggtg ttcatatttc gctacttcac
     7381 ggtcaaagtg cgggtcgttc gttctggtct tcgatagttt tttgattgac attttctgac
     7441 gttccattag aatcccgcct tctctggaac tgcccaggtg gcgaaattgg tagacgcgct
     7501 aggttcaggt cctagtggtg gcaacactgt gggggttcga gtcccttcct gggcaccaaa
     7561 caacaatccg agatattcca gacaggttct tcaagccgca aaaccaccag gttctgcggc
     7621 ttttttgctt cgacagccgt gcagcttagc ccatccaaga tgaactttcc ttggcctatg
     7681 cgtcagtata cgttcaatgc ttggtatacg gtctgccgaa ttaccatgca cccgatcggg
     7741 tggagcctta cagaatggaa aagatcgcag cgatcgtgat cggcgccggt gtcgtcggac
     7801 tggcgtgcgc acgcgcgctg gcccaacgcg gtatcgcgac aatcattctc gaacggcacg
     7861 atgcgttcgg caccgaaacg agcgcccgca atagcgaagt gatccacgcc ggtctgtatt
     7921 acccgtccgg ctcgctcaag gctcagctgt gcgtcgccgg acggcgtctc ctctatgact
     7981 attgcgccac gcacggcgtc cggcaccgcc gttgcggcaa actgatcgtc gctacttcat
     8041 cagaacagga agcccgactg gacgcactgc gtcggcaggg cgaaaccaat ggcgtcgacg
     8101 atcttcaacc acttggcgcc ggcgatgcgc gccgactgga acccgagctc agatgcactg
     8161 ccgccctgct ctccccgtca accggcatca tcgacagcca tggcctgatg ctggcgctgc
     8221 tcggtgatgc cgaacgcaac ggcgcgactc tggctgtatg cagcccggtg tgcgccggcc
     8281 gcatcgaaga cgacgggatg attctggaga ttggcggaaa ggatccgacg caactcaaag
     8341 ccgacatcgt gatcaacgcc gccgggctct acgcgacgca ggtcgccgcc tccttggagg
     8401 ggtttccggc agagcagatt ccgccggcct tctatgccaa aggcaactac tgtgccctca
     8461 ccgcgaaatc accttttacg cggctgatct acccgctgcc ggaggctgga ggtctgggca
     8521 ttcacctgac gctcgacctt ggcggacagg cgcgctttgg ccccgacgtc gagtggctgc
     8581 cagcgctcgg tgacaaaccc gactatcgtg tcgatccagt acgcgccgat gcattctgca
     8641 acgagattcg ccgttactgg ccgggcctgc atgatggtgc gctgaccccg gcctatgccg
     8701 gtatccggcc gaaaatttca gggccgcagg acgcggcggc cgactttctg atccaggggc
     8761 aagagtcgca cggcgttccg cacctgatca acctcttcgg tatcgagtcg ccggggctga
     8821 ctgcttcatt ggccatcgcc gaacacgtcg ttgaacggct cggatagctg cctccgcaca
     8881 gactagccgt gcccgcaatc ggacaggaag cccagcaact cctcgcgcag gggatagtag
     8941 tccggatgtt cgagcagcat cttgcgggta cgcggcctgg gcaggttgat ttccagcact
     9001 ttgccgactt tggcgcgtgg accattggtc atcatcacca cacggtcggc cagcagaatc
     9061 gcttcgtcca cgtcgtgggt gaccatcatg gcggttagct ggctacggct ccagacttcc
     9121 atcagcactt cctgcagatc ccagcgggtg agcgagtcga gcatgccgaa aggctcatcg
     9181 agcagcagca acttgggaga tagcgcgaag gcacgtgcaa tgc
//
LOCUS       DAORPM010000080         9159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1791_length_9159_cov_8.281085, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000080 DAORPM010000000
VERSION     DAORPM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1791_length_9159_cov_8.281085"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1760
                     /gene="nifL"
                     /locus_tag="PLD53_05480"
     CDS             228..1760
                     /gene="nifL"
                     /locus_tag="PLD53_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HRC60395.1"
                     /translation="MSALTRPKLSSVKQKPELPPEIYRQAVDQADLAISITDIKANIL
                     FANEAFARVTGYDAEEIIGKNESILAAEQQTPPRLYQSMWDDLAAQRPWSGKLLNRKK
                     NGELYLAELTISPVVNSQGNTTHFLGMHRDVTELHRLERMVRNQKVLIESVVDSAPIA
                     FALLDQTGRVILDNQEYKKLLSDLQVKEPAHTLLDSLLPLWHDALVKEPERCVFARRE
                     ARIDRVGGRPRWLSVSASVIEIHSDDEENCFNIVQQRGLLLAISDVSSLREEQERARA
                     AVMQAVLADEERTSAIREGLSAAIFRLEEPMNVMASAVSVLYRRDPLSAEALDRALAA
                     SREHLELLRQVIPHHAQEIEVHTNLNEVLRDVLEISTQRLLGAGIVVDWKPAITLPPI
                     IGRPLQLCMLFKALVDNAIEAMNIKGWKQRELSLSSAVDDQCIVITISDSGPGIPSER
                     RHKAFEPFVTDKNGTGHHIGTGLCRAQQVVADHGGIIDLEASASGGCRVIVEFRLDGD
                     PI"
     gene            1779..3386
                     /gene="nifA"
                     /locus_tag="PLD53_05485"
     CDS             1779..3386
                     /gene="nifA"
                     /locus_tag="PLD53_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HRC60396.1"
                     /translation="MSELTLPSADAMPNCGYCRTHELNIMLLAALYAVSRVLSRSLAF
                     NESLRDVLRILHDEAGLTCGLISVVDQPTGNLTAHVLHHPAVIGNVQYQPGEGILGLM
                     LEKPRTVMLSRIADEPRFLNRLDIYQQELPFIAAPIRVGGNLQGILAVQPGSPDDGLL
                     EERTQFVEMVANLIGQSLRLSLEVEQEKSTLVQERDLLRRTVRHQFGFDSMVGRSATM
                     RRVFDQARLVAKWDTTVLIRGETGTGKELIANAIHYNSARARNTMVKLNCAALPENLL
                     ESELFGHERGAFTGAVEARKGRFEQAHGGTLFLDEIGDVSSAFQAKLLRILQEGEFER
                     VGGNRTIKVDVRFIAATHRDLETAVEMGDFREDLFYRLNVMPLFLPPLRERIEDIPEI
                     ARHLLGKIGSRQKRKLNLTDMAQRRLVSHSWPGNVRELENCLERAAVLAEDGQLDVDL
                     IRFPSTRERTTHRPLRSLPSAATGMGSSSNSEFDIDDPDLSEKERVIAALEQAGWVQA
                     KAARILGMTPRQIAYRIQTLNIEVKQF"
     gene            3439..3708
                     /locus_tag="PLD53_05490"
     CDS             3439..3708
                     /locus_tag="PLD53_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HRC60397.1"
                     /translation="MIHTAYEIGDMVYAAEDILNDGGMPGVEEMEGLIAPAGTRGVVV
                     NSGVAELDKRQAIYLVQFENGPDKTLGDPVGCLPEDLTQNESVPS"
     gene            3762..4532
                     /gene="modA"
                     /locus_tag="PLD53_05495"
     CDS             3762..4532
                     /gene="modA"
                     /locus_tag="PLD53_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC60398.1"
                     /translation="MIRPARRRMLPCVLFLLALLCTSPLRADVVQVAVAANFTAPMQK
                     IAAEFERDTGHRASLSIGSTGKFFAQVANGAPFDVFLAADVETPARLEQDSYAVSGSR
                     FTYAFGRLALWSAKKDKVDSQGEVLKKGEFRHLALPNPKFSPYGAAAIGIMQQLGVEA
                     TLQAKFVLAENVSQVYQFAASGNAEVAFIALSQIWKDGKLATKGSAWVIPPEMHAALR
                     QDAVLLNHGRDNPAASALLGYMKGDKARSIIKSYGYDL"
     gene            4536..5222
                     /gene="modB"
                     /locus_tag="PLD53_05500"
     CDS             4536..5222
                     /gene="modB"
                     /locus_tag="PLD53_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRC60399.1"
                     /translation="MPLAMDSSDLSAVWLTLKLATVVTVLLLVIGTPIAWWLARTNSA
                     WKGAIGAIVALPLVLPPTVIGFYLLVAMGPNGPVGHLTQSLGLGLLPFTFPGLVIASV
                     FYSLPFVVQPIQNAFEAIGNRPLEVAATLRASPWDAFWSVAVPLARPGFISGAILGFA
                     HTVGEFGIVLMIGGNIPDQTRVVSVQIYDRVEAFEYAQAHWLAGAMALFSFAVLLALY
                     SFNPARRRSR"
     gene            5219..6346
                     /gene="modC"
                     /locus_tag="PLD53_05505"
     CDS             5219..6346
                     /gene="modC"
                     /locus_tag="PLD53_05505"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HRC60400.1"
                     /translation="MKHANKVKEANKADKKIRARFCVERGTFKLDVDVCLPGYGVSAL
                     FGHSGSGKTTCLRAMAGLERAPGGYFAIGDDVWQDEAKNRFVPPHQRELGMVFQDASL
                     FSHLSVRGNMEFGQKRVQRRALGGEHRFVLPEVAELLGITHLLDRSTDQLSGGERQRV
                     TIARALLSSPKILLMDEPLAALDMKRKLEILPYLERLREELSMPIVYVSHAPDEVARL
                     ADHLVLLEQGHVVASGPLNQVLSRIDLPDAFADDAGVVIKGYVAGHEADDLTRLEFSG
                     GRIYISRRHEPVGTVLRCRVHARDVSLTLMTQQHTSILNSVSARVVDLAQTNTPGHVL
                     VRLDVADDPLLARITRRSAEKLDIRPGLTLTAQIKSVALLG"
     gene            6683..7975
                     /locus_tag="PLD53_05510"
     CDS             6683..7975
                     /locus_tag="PLD53_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRC60401.1"
                     /translation="MPHQNTSDAPRIYNLFPLLAGSTENWESHLPRIASMGFNWVFIN
                     PFHYPGFSGSLYAVKDYYRLHPLFQGGSQEEASTLLGRFISAAHGHGLRVMMDLVINH
                     TSKDSLLAEAHPEWFAHEPDGSLRSPFAVDPDDVDNVAKRTVWGDLADIDFENTPDRE
                     GLVSYWQELVRHYVKLGFDGFRGDAAYKIPGDVWAPIIAVARDSNPDAMFFAETLGCQ
                     PKEVLQLKAAGFDYLFNSSKWWDFRADWLMKQYALFRAIAPSIAFPESHDTERLAEEN
                     SGDERVSRLRYLFAAAFSSGLMLTMGYEYGFRRKLNVVTTRPEDWETPSFDLSRFIGE
                     VNAMKATTPVLNEEGPQELLSGADDPVAALIRRGERTASVLTLINPDANTESRFSTAD
                     AAKILGCSPQQMRELLPGPTNSDWITLPPLAIRLFTAD"
     gene            8487..>9159
                     /locus_tag="PLD53_05515"
     CDS             8487..>9159
                     /locus_tag="PLD53_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60402.1"
                     /translation="MTNVLAFEHNPLNDDLPPEGTRRIIAGQERVLYSNYWIKTYLVP
                     GDTLEAKKRLIEALTRRLFNHTEHGLNIPGTRLADAQRSYLAETDPPRRRVKAAMLAG
                     ALFNRATDIFRRLVELQSDGVEIHSDDALMRECGQCLLDAMELGHFVLHRSGEEGIDE
                     LWGEPFRAFSIPLEDFYESRYIKIGQSMRDIDRIAEAMRENFSRIPGFENIASPIHNF
                     VDAAKI"
BASE COUNT         1920 a         2828 c         2613 g         1798 t
ORIGIN      
        1 cctatcgatc caagcatttg ctggaaatgc accagcttgg ggaaaaacaa agggctatcc
       61 aatgttgcaa acccgacagg gatttcgtcg caattacccc gtcagtgttg tttttcaagg
      121 ccgtcagcgc acccagccgg ctcgttttct tgatcggcac ggaagctgct tgagaggagg
      181 tagaaatgcc gatcaaaaac tagtcccgtg ctttcaggag caatgccatg tccgccttga
      241 cgcgcccaaa gttatcctct gtaaaacaga aacccgagct gccgcctgaa atctatcgcc
      301 aggcggtgga ccaggccgat ctcgccatct cgatcaccga catcaaagcc aacatcctgt
      361 tcgccaacga agcctttgcc cgcgtcaccg gctatgacgc ggaggaaatc atcggcaaga
      421 acgagtcgat cctcgccgct gagcagcaaa cccccccaag gctgtaccag tcgatgtggg
      481 acgatctggc ggcgcaacgg ccatggtccg gcaagctgct taaccgcaaa aagaacggcg
      541 aactctactt ggccgagctg accatttcgc ccgtcgtcaa tagccaggga aacacgacac
      601 attttcttgg catgcaccgc gacgttaccg agctgcatcg ccttgagcgc atggtccgca
      661 atcagaaggt cctgatcgaa tcggttgtcg attcggcgcc gattgcgttt gccctgctcg
      721 accagactgg ccgcgtcatt ctcgacaacc aggaatacaa gaaactgctc agcgatctgc
      781 aagtcaagga acctgcccat accctgctgg acagcctgtt gccgctgtgg cacgacgcgc
      841 tggtcaaaga acccgagcga tgcgttttcg cccgccgcga ggcacgtatt gatcgagtcg
      901 gtggccgtcc gcgctggcta tcggtcagcg cctcggtgat cgagattcac agcgacgacg
      961 aagaaaactg cttcaatatc gttcaacagc gcggtctgct gctggcgatc tccgatgtca
     1021 gctcgctgcg cgaagagcag gagcgcgccc gcgcggccgt catgcaggct gttctggccg
     1081 acgaggaaag aacttcggct atccgggagg gcctctcggc cgctattttc cggctggaag
     1141 aaccgatgaa cgtgatggct tcggccgtct cggtgctgta tcgacgcgac ccgctcagcg
     1201 cggaagccct ggaccgcgca ttggcggcga gccgcgaaca ccttgaattg ctgcgccagg
     1261 tcatcccgca tcacgcgcag gaaatcgagg tgcataccaa tctcaatgaa gtattgcgcg
     1321 atgtgcttga aatcagcacg caacgcctgc tcggcgccgg catcgtggtc gattggaaac
     1381 cggcgataac gctgccgccc atcatcggcc gcccactgca actttgcatg ttgttcaagg
     1441 cgctggtcga caacgccatc gaggcgatga acatcaaggg ctggaaacaa cgcgagctga
     1501 gtctttccag cgcagtcgac gaccagtgca tcgtgatcac catcagcgac agcggccccg
     1561 gaattccgtc cgaaaggcga cacaaggcat tcgagccctt cgtcaccgac aagaacggca
     1621 ccggccatca catcggcacc gggctttgcc gcgcccagca ggtcgtggcc gaccatggcg
     1681 gcatcatcga tctggaagca agcgcaagcg gcggttgtcg ggtcatcgtt gaatttcggc
     1741 tggacggcga cccgatctga gccacgactg agaacggcat gtccgaactt accctcccct
     1801 cggccgacgc gatgcccaac tgcggctatt gccgtacgca cgagctgaac atcatgctgc
     1861 tcgcggcact ctatgcggtc agccgtgttc tgagccgatc gctggccttc aacgaaagcc
     1921 tgcgcgacgt gctgcgtatc ctgcacgacg aggcgggact gacctgcggc ctgatcagtg
     1981 ttgtcgacca gcctacgggc aaccttacag cgcatgtact ccatcacccg gctgtcatcg
     2041 gcaatgtgca gtatcagcct ggtgagggca tcctcggcct gatgcttgaa aaaccgcgaa
     2101 ccgtgatgct ctcgcgcatc gccgatgagc cgcgcttcct caaccgcctc gacatttacc
     2161 agcaggagct ccccttcatt gccgcgccga tccgggtcgg cggcaacctg caaggtattc
     2221 tcgccgtaca accgggatcg ccggacgatg gactgctgga ggaacgtacg caattcgtcg
     2281 aaatggtggc caacctgatc ggccagagcc tgcgcttgtc gctcgaagtg gaacaggaaa
     2341 aatcgaccct ggtccaggaa cgggacctgc tcaggcgtac cgtccgccac cagttcggtt
     2401 tcgacagcat ggtcggccgc tcggcgacga tgcgccgtgt tttcgaccag gcgcgtctgg
     2461 ttgccaaatg ggacaccacc gtgctcatcc gcggggaaac cggcaccggc aaggaactga
     2521 tcgccaatgc cattcactac aattcggcac gagcgcgcaa tacgatggtc aaactcaact
     2581 gcgctgcact gccggaaaac ctgctcgaat ccgaactctt cggccacgaa cgcggcgcct
     2641 tcaccggcgc cgtggaagca cgcaaggggc gtttcgaaca ggcgcatggc ggcaccctat
     2701 tcctcgatga aatcggcgac gtctcgtcgg ctttccaggc caagttgctg cgcattttgc
     2761 aggaaggcga attcgagcgc gtgggtggca accggacaat caaggttgac gtgcgtttca
     2821 tcgccgccac tcaccgcgat ctcgaaaccg ccgtcgagat gggcgatttt cgcgaagatc
     2881 tgttctacag gctcaatgtc atgccgttgt tcctgccgcc gctacgcgag cgcatcgaag
     2941 acatccccga aattgcccgc catctgctcg gcaagatcgg cagcagacag aagcgcaaac
     3001 tgaacctgac cgacatggcc cagcgccggt tggtcagcca ctcctggccg ggcaatgtgc
     3061 gcgaactgga aaattgcctc gaacgtgccg ccgtgctggc cgaagacggc cagctcgatg
     3121 tcgacctgat ccgcttcccc agcaccagag aacgcacgac gcaccgcccg ctacgcagtc
     3181 tgccatctgc cgcgaccggc atgggtagca gctcgaactc cgaattcgat atcgacgatc
     3241 ctgatctgtc agagaaggaa cgggtgattg ccgctcttga acaggccggc tgggtgcagg
     3301 ccaaagccgc ccgcattctt ggcatgacgc cgcgccagat tgcctatcgg atccagactt
     3361 tgaacatcga agtaaagcag ttttagtaga cggccgacag aatcggcaag caggcttttt
     3421 tgacgaaagg aggaactcat gatccacact gcatacgaga ttggcgacat ggtgtatgcc
     3481 gccgaagata tcctcaacga cggtggcatg ccgggtgtcg aggaaatgga aggtttgatc
     3541 gcccccgcgg gtacgcgcgg cgtcgttgtg aattctggcg tggccgagtt ggacaagcgt
     3601 caggcaatct atctggtgca gtttgaaaat ggcccggaca agacgcttgg cgacccggtc
     3661 ggctgcctgc ccgaggactt gacccagaac gagtccgtgc cttcatagag caactcgtga
     3721 acaccttaaa accaacccaa gccactgaca tgaacatgac catgatccga ccagccagac
     3781 gcagaatgtt gccgtgcgta ttatttctac tcgcgctctt gtgcacgtcg ccattgcggg
     3841 ccgacgtggt gcaggtggcc gttgccgcca atttcacggc acccatgcag aaaatcgctg
     3901 ccgaattcga acgtgacacg ggccacaggg ccagtttgtc catcggctcc accggcaagt
     3961 tcttcgcgca ggtcgccaat ggtgcgccat tcgacgtgtt tcttgcagcc gacgttgaaa
     4021 cgccggctcg actggagcag gactcttacg ctgtcagcgg cagccgcttc acctatgcct
     4081 ttggccgcct cgccctgtgg tccgcaaaga aggacaaagt cgatagccag ggcgaggtac
     4141 tgaaaaaagg cgaattccgc caccttgcgc tacccaatcc caagttttcg ccctacggcg
     4201 ctgccgctat cggaatcatg caacagctcg gcgttgaggc caccttgcag gcgaaattcg
     4261 tacttgcgga gaatgtttct caggtctacc aattcgcggc gagcggcaat gcggaagtcg
     4321 cgtttatcgc tctctcgcaa atctggaaag acggcaagct cgccaccaag ggctcggcct
     4381 gggtcattcc gcccgaaatg cacgcagcgc tccgccagga tgccgtgctg ctcaatcatg
     4441 gccgcgacaa tccggcggcc agcgccctgc tcggctacat gaagggtgac aaggccagga
     4501 gcatcatcaa atcgtatggt tacgatctat aaggtatgcc cttggccatg gacagctctg
     4561 atctttccgc cgtctggctg accttgaaac tggccaccgt ggtcaccgtg ctcctgcttg
     4621 tcatcggtac accgatcgcc tggtggctgg cacgtacgaa ttcggcctgg aagggcgcaa
     4681 tcggagcaat cgtcgccctg ccgctggttc tgccgccgac cgtcatcggc ttctacctgc
     4741 tcgttgcgat ggggccgaat ggtccggtcg ggcatctgac acagtcgctc ggcctgggcc
     4801 tgctgccctt cacctttccc ggcctggtca tcgcctcggt gttctactcg ctgccttttg
     4861 tcgtgcagcc gatacagaat gccttcgaag ccatcggcaa tcgcccgctg gaagttgccg
     4921 ccacgctgcg cgccagcccc tgggacgcct tctggtcggt ggccgttcct ctggcacgac
     4981 caggtttcat aagcggcgcg atccttggct ttgcgcacac ggttggcgaa ttcggcatcg
     5041 tgctgatgat cggcggcaac atccccgacc agacccgcgt cgtttcggtc cagatctatg
     5101 accgcgtcga agcgttcgaa tacgcccagg cccactggct ggcgggtgcc atggcactgt
     5161 tcagctttgc cgtgctgctg gcgctgtaca gcttcaatcc ggcgcggcga cggtcccgat
     5221 gaaacacgcg aacaaagtca aggaagccaa caaggccgac aagaaaattc gggccagatt
     5281 ctgcgtcgag cgcggcacgt tcaagctgga cgtcgacgtg tgcctgcccg gctacggcgt
     5341 cagcgcacta ttcggccact ccggttcggg aaagacgacc tgcctgcgag cgatggccgg
     5401 gctggagcgc gcaccgggcg gctatttcgc catcggcgat gacgtctggc aggacgaagc
     5461 gaagaatcga ttcgtaccgc cgcaccagcg tgagctgggg atggtatttc aagacgccag
     5521 cctgttttca cacctttccg ttcgcggcaa catggaattc gggcaaaaac gtgtccaaag
     5581 acgtgccctg ggtggtgagc accgctttgt cctgcccgaa gttgccgaac tgcttggcat
     5641 cacgcatctt ctcgaccgct ccaccgacca gctttccggc ggcgaacgcc aacgcgtgac
     5701 gattgcccgc gcattgctca gttcgccgaa gatcctgctg atggacgaac cgcttgcggc
     5761 gctggacatg aagcgcaagc tcgaaatcct tccctacctg gagcggctgc gagaggagct
     5821 ctcgatgccg attgtctacg tgagtcacgc gccggatgaa gtggcgcgac tggctgatca
     5881 tctggtgttg cttgagcagg gacacgtggt ggccagcggc ccgctcaatc aggtactcag
     5941 ccgcatcgac ctgccagacg ccttcgccga tgatgccggg gtggtgatca aaggctacgt
     6001 tgccgggcac gaagcggacg atctgacccg gctcgaattc tccgggggcc ggatttatat
     6061 ttcgcggcgg catgagccgg tcggtacggt actgcgctgc cgtgtccatg cgcgcgacgt
     6121 cagcctgacg ctgatgacac agcagcacac gagcattctc aactccgtat ccgcccgggt
     6181 cgtcgaccta gcgcagacca atacgccggg ccatgtactg gttcgtctcg atgtcgccga
     6241 cgaccccctg ctcgcgcgca tcacccgacg gtcggcggag aagctcgata tacgcccagg
     6301 actcaccctg acggcacaaa tcaagtccgt ggccttgctg ggttgatcag gtttccgacg
     6361 actgagaact gcacctgttt tcagggtaac agacacggat ttcaggataa atcagacgaa
     6421 gctggcggag aaactggcgg gaaatcacgg ttgacaatgg gcggaaggtg cagggatact
     6481 tggcggaagg tcgttgaatt tcctatcctt cattctgtcg tattcgcaca cggtatcggt
     6541 ttctttcagt ccgcacctga ccatccgccc ggacaggttc tgaatcgctc cgtgccccac
     6601 cagaccaacc gaatggaaac tgatcaagtc gccagtaatg cggcgttttc ggttgccgat
     6661 tcatacccac ccatcggacc atatgccaca tcaaaacacg agcgacgcac cgaggattta
     6721 caaccttttt ccccttctgg caggcagcac cgagaattgg gagtcgcacc tgccccgtat
     6781 cgccagcatg ggtttcaact gggtcttcat caacccattc cattacccgg gtttttcggg
     6841 aagcctctac gccgtcaagg actactatcg cctgcacccc ctctttcagg gtggtagcca
     6901 ggaagaagcg tctaccctcc tcggtcgttt cattagcgct gctcacggcc atggtctgcg
     6961 ggtgatgatg gatttggtga ttaaccatac gtcaaaggat tccttgctgg cggaagcgca
     7021 cccggaatgg ttcgcgcacg agccggatgg cagtttgcgc tcccccttcg ccgtcgatcc
     7081 cgacgacgtg gacaacgtcg caaagcgtac cgtctggggc gatctggccg atattgactt
     7141 cgaaaacacg ccagaccgcg agggactggt cagttattgg caggagctcg ttcgtcacta
     7201 cgtaaaacta ggcttcgacg gtttccgtgg cgacgcggcc tataaaattc cgggcgacgt
     7261 ttgggcgcca atcatcgcgg tagcccgaga cagcaatccg gatgcaatgt tcttcgcgga
     7321 aacgctgggc tgccagccga aggaggtact gcaactcaag gcagccggct ttgactacct
     7381 gttcaattcc agcaagtggt gggatttccg tgccgattgg ctcatgaagc agtacgctct
     7441 atttcgtgcc atcgccccct ccatcgcctt ccccgaaagc cacgacaccg aacgcctggc
     7501 cgaggagaac agcggtgacg agcgtgtttc ccgcctgaga tacctgttcg cggcggcctt
     7561 ttccagcggt ttgatgttga ccatgggcta cgaatacggc tttcgtcgca aactcaatgt
     7621 ggtgactact cgcccggagg actgggaaac gccaagtttc gacctgagcc ggttcatcgg
     7681 cgaggtcaat gccatgaagg caaccacccc agtgctcaat gaggagggcc cacaggaact
     7741 cctgtccggt gcggacgacc ccgtcgctgc gctgatcaga cgtggcgagc gcaccgcaag
     7801 cgtgctgact ctgatcaatc cggacgccaa tacggaaagc cgcttcagca ccgccgatgc
     7861 ggccaaaatc ctgggttgct caccacagca gatgcgggaa ctgctgcccg gcccgacaaa
     7921 ctccgactgg atcaccctgc cacctttagc cattcgcctg ttcacagcag actgagacaa
     7981 caaggattca cgcccaacgc aaatccgaca tcgcggagag ctgcggcttc tcagcaaaga
     8041 ccgctcccgt tcccgccgtg ccgattactg ggaacagaaa ctcttaccgg cgcaaacggg
     8101 tgcgccatgc cttgatcatg atgtcctgat cgccgcactg gcacgagagg cgggacggga
     8161 tattgcgcct accaggagca attcggcctg aacggcgcgg cgacgcgcgc atccaccaac
     8221 catcgatcac cactgaccgc ccaagccata agcgttttga ctggtggttt tggggcacaa
     8281 aatgcgtgcg tactctaatc acgcacgcga tcacggcgta gcgcaccaga agcgggcggg
     8341 aagcactctt gcgatcaaca ggcagacgcg gcattccacg cctattccac gtcatttcag
     8401 tgccttggtg aaatcgcaaa agtcggcaaa ctcccgcatg atttttgctt tatttattgt
     8461 gtgtttccta aaaatagtgg cttaccatga ccaacgtcct tgcctttgaa cacaacccgt
     8521 tgaacgacga tctgcctccc gaaggcacgc ggcgcatcat cgccggacag gagcgggttc
     8581 tttacagtaa ctactggatc aagacgtatc tcgtccccgg cgatacgctg gaggccaaga
     8641 aacggctgat tgaagcgctg acccgacgcc tgttcaacca taccgagcac ggcctcaaca
     8701 tccctggcac ccgcttggcc gatgcgcagc ggagttatct ggcggaaacc gatccaccgc
     8761 ggcgccgggt caaggcagcg atgctggcgg gcgcgttgtt caaccgggcc accgacatat
     8821 ttcgcaggct ggttgagttg cagtccgatg gcgtcgaaat ccacagtgac gacgcattga
     8881 tgcgtgaatg cggccaatgt ctgctcgacg ccatggaact aggccatttc gtcttacacc
     8941 gcagcggcga ggaaggcatc gacgagctat ggggcgagcc gttccgcgcc ttctcgattc
     9001 cgttggaaga cttttacgaa agccgctaca tcaagatcgg gcagagcatg cgcgacattg
     9061 accgcatcgc cgaggcgatg cgcgaaaatt tctcccggat tcctggtttt gaaaacatcg
     9121 cttcgccgat tcacaatttt gtcgatgcgg ccaagataa
//
LOCUS       DAORPM010000081         9014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1841_length_9014_cov_10.514120, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000081 DAORPM010000000
VERSION     DAORPM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1841_length_9014_cov_10.514120"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLD53_05520"
     CDS             <1..560
                     /locus_tag="PLD53_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRC60403.1"
                     /translation="FDEGGRKGYKFWKSNPDKLFTADEMIAIYADWCNKYPIVSIEDG
                     LDQSDWDGYVKFTQALGKKVQIVGDDFFVTNPVRLKKGIDMGACNAILIKVNQIGTVT
                     ETLDAIKMAQKAGYGVISSHRSGETEDSFIADLAVGTGAGQIKTGSLSRTDRICKYNQ
                     LIRIEEQLGDKAIYKGLKSIKGAGY"
     gene            931..2634
                     /locus_tag="PLD53_05525"
     CDS             931..2634
                     /locus_tag="PLD53_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HRC60404.1"
                     /translation="MAQLTPMAKGLITVIVLGIAGSAAWNFGLKELWQNRGGETSTME
                     KSAAKAPSANDPAASSAKAAATATKLDGSAALGSAANPLRVSIVSFHGYAPALVANGN
                     SLTTQQNSIYSAKGVNVQFVIQDDIPTLSTIFESGAAQCAWRTSDFWAQEQPNLRNAG
                     LDGRAVMIVDNTRGGDAVIARNPAIASIEDLAGRSVALLQFTPSHGMLIDAIENSSLS
                     ARKKDSIRMVFINAEEGTAGVRAAFESANVEAAVLWDPDLALAQRAVKGSRIVYSTKT
                     ATNLIFDVMVCDSRLLAKPEGQAVIQKFVEGWMEGVQAARSNPDNAVDALVNTEEFFK
                     LLADKEGKPFIKSLFSNLVWTGVEDNARILGLAGGTNHYERVYRRFDEIYRKAGALAN
                     PKSPVITPQDSFDYRFIKSMLARDKSASEAAAKPQETFSQAALKDATQNAAIVTKPVM
                     VGFSTGTAELTKRAQKTIDEEMVPFIENNGKAYFEVSGNSDSTGSREVNLRISGARAK
                     AVVDYLNVQWEFPRDRFKIVGNGPDRPLCNESNPAGEGLSLEECRAMNRTTRIGVFGG
                     R"
     gene            2638..3435
                     /locus_tag="PLD53_05530"
     CDS             2638..3435
                     /locus_tag="PLD53_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60405.1"
                     /translation="MADFWNPYASPEAKHARVLGFGSVLAVLIIWSVLSGFGLVGPTR
                     LPAPWDVLKAFSYLAWYDGNSMLLTAALWSVGRLAVAGFFVILIGIPIGVAMGASPRV
                     NAVLSPLVDPFRSAPVVALLPILVMWLGIGEAMKVAFLFIGAVVYLIPMVRDAIQAVP
                     QSYWIGARDLGATPWECIQKSVIPMAMPRIADGVIVAFSVMWTYITVAEYVNARVGLG
                     QLIQNARRFSAWDQVFAGIIVIVGLALLTYQLMVFAKRRLFPWETQQ"
     gene            3432..4295
                     /locus_tag="PLD53_05535"
     CDS             3432..4295
                     /locus_tag="PLD53_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC60406.1"
                     /translation="MIAAGSVTDGIGKKPDITVTLKNILQEYPAPGGGTTRVIDGVSV
                     DFSKPGINMLLGPSGCGKSTLLHMMGGVRPMGVKTPTSGTILIDGVECDGPHDDSVMV
                     FQRYANRPDLTVFENVAFPFRLKLWKKKVPDAEWRQRVQEMLKAVGLADKQKLRPAQL
                     SGGQNQRVALARALVLRPRILLMDEPFGALDAQTREEMQRLLIELYNTWPCLVIFVTH
                     DVTEALILGDRVIVLSSQPAKVANDFTIDVPRPRSTDWLRSSQATQLEEGILKQLHKA
                     SLGRGSVRVTV"
     gene            4306..5208
                     /locus_tag="PLD53_05540"
     CDS             4306..5208
                     /locus_tag="PLD53_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60407.1"
                     /translation="MADNDSAYLSEMLTSQGNLYALLGSTALGVVLSIPFGFGVGAIP
                     LIAFAAGEIIAAMYLPSSIAFRDRVDRKLRRQARLAARKQLLQEIESRCAKGAISFQQ
                     LSRTYTRMSERIAALYSHAESGSKRLSLQDVERLEDAGTDYLCVWLAVLVMEDRAEQI
                     DLADIAARIDAIDRELKSPRPGSDMRQLQKARAEYVAIIERYHRMSSRKRALEAAMVS
                     MPDQMEEIYQTIMTTSATEDVGSRLGDAIAKLRLQEDIEAELAEGSSFVSEPNNVVPG
                     IIAQASRSAARKPQLAAVNRGQNH"
     gene            5235..5948
                     /locus_tag="PLD53_05545"
     CDS             5235..5948
                     /locus_tag="PLD53_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HRC60408.1"
                     /translation="MADINFILRLSNLWSGFVSLWITDVEKRHPEIAYQNAIDSMIEK
                     YGKLKSATASIMRRREDVSARLGRDQQELAAINADLNVALATGQDELGIVLIQKKNAL
                     EASIKSLEQDMEQARTDADEAKSSLVQVKGEIQKLKEEKDRMLAQMLSAEARLKIQNQ
                     LEGLSVDAEVRALDNVREHIKNTVAEAKMGSELRDSDLDVQLNKLRQSSGAITARQQL
                     EEMKRVKAAQATGSSGKSM"
     gene            5948..7786
                     /locus_tag="PLD53_05550"
     CDS             5948..7786
                     /locus_tag="PLD53_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC60409.1"
                     /translation="MNSPPNEAPNRHELPAWAETVRQKYLAGEASVFVLYGNVFDRTL
                     INDKPYALVDFLSEILLKDNKQRVFEVSIDRGVRALHQASLAEKEEIYRQLEGRGLAG
                     IFESLERRMREQPSNAVILPYAGTLLPAAELHLLSLEERGAFTTLHRWSLDDDLSAKD
                     NVIILITESLAEINSGLLANPRVAAVELPIPERAARDRAIRYYAPKMPDEQVLVLADQ
                     TAGLRIIQLASIVASEGPQGLNEGQRSKLIGELLQGTPNAAERSAKLAAITSGMTPDE
                     IRQLVDPTRPLPDAEDPYSEVLEVVRKRKRELIEKECSGLIEFIEARHGLEAVGGNDL
                     IKAELMEIAHLIKGGERGRAPMGLLAVGAMGSGKTFVIKAFLKEAGLSGIALKNFRSK
                     WVGSTEANLERVLATVKAMGPIALVIDEGDRSFGNQSEDSDGGTSSRVIARLKEFMSD
                     SDNRGQVLFILMTNRPDKLDTDIKRPGRLDRKIPFFYAETGAERAAIVVAILRRYRVE
                     CPIAPSAWNALCEQLEGYSNADLEALALLAVEFAGREGGVLSETVFARAVEDFMPPRE
                     TTMLSYMEMLAVFETSRRSLLPPRYRSLTPQEVQDQLSALRRQLNH"
     gene            7798..8253
                     /locus_tag="PLD53_05555"
     CDS             7798..8253
                     /locus_tag="PLD53_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRC60410.1"
                     /translation="MFPLKTDTPLTQDQVVTVTRLMLHVAHVDGDKTAEELELIRAFY
                     EGCVEAGNGWQTFDSLDGKSGSPDVAASDFADQGQREMALAICLMVAWADGAFSARED
                     ESVRGIADKLGVGGERFAQVLALVKDHMLAQLAGLPDVGSIVAVAKELG"
     gene            8267..8944
                     /locus_tag="PLD53_05560"
     CDS             8267..8944
                     /locus_tag="PLD53_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60411.1"
                     /translation="MKGILGFLARANLVELTEEERLKAVADGHAETPGSVPPPEEPVA
                     QAPPPPPLNIEECEIADDRPLEEIFSAAGVPESPYPVEKMLRLLDGLRAMDAATRKAA
                     VLAMDAADDNWQIADCVGDAERKIAAIEAYKQHLAAQVEGSEQQVSMQIAELKSALEN
                     TTAAIRRQISELEELLQREVAKEAQQTTSLEAGLRATREAAARETRRMAAEVERLSEI
                     TLQFGPG"
BASE COUNT         1941 a         2488 c         2768 g         1817 t
ORIGIN      
        1 tgttcgacga aggcggccgc aagggctaca agttctggaa gtccaacccg gacaaactgt
       61 tcactgccga cgagatgatc gcgatctacg ccgattggtg caacaagtac ccgatcgttt
      121 cgatcgagga cggtctcgac cagagcgact gggacggcta cgtcaagttt acgcaagctc
      181 tcggcaagaa ggttcagatc gttggcgacg acttcttcgt gaccaatccg gttcgcctga
      241 agaagggcat cgacatggga gcctgtaacg ccattctgat caaggtcaat cagatcggca
      301 ccgtgaccga gacgctggat gccatcaaga tggcacagaa ggccggttat ggcgtgatct
      361 cctcgcaccg ctccggtgag acggaagatt ccttcattgc cgacctcgcc gtgggtacgg
      421 gcgccggcca gatcaagacc ggctcgctgt cgcgtactga tcgtatctgc aagtacaatc
      481 aactgatccg catcgaagag caacttggtg acaaggccat ctacaagggc ctcaagtcga
      541 tcaagggcgc cgggtactga gcaaacctcc tggtagaaaa agcatcgcct cggcggtgct
      601 tttttttcct ctccacccgg atgagctgct gaaatctcgt cgcgacaaca tctcggacat
      661 aggcgcattc atcctttggg cataagttgg caacatccgg acgctccggc aacgctggcg
      721 atcggagggc cgaagagcgt ttcgtgacgt caggtcgcac gctgatcgca tacaattcag
      781 gttccccttg acagtcttcc gggggagcgg cagcatcgcg acagcgtagt tggcggaaat
      841 ccggggagcc cgaacgtttc ccaggctttg gcatatctag gctcggctgt tgtccatacg
      901 agccgagtac taactgttca ggagaatgca atggcgcagt tgaccccgat ggcaaaagga
      961 ttgattaccg tgatcgtgct cggcattgcc gggtccgcgg catggaattt cggcttgaag
     1021 gagctatggc agaaccgcgg aggcgagacg tcgacgatgg agaaatccgc cgccaaggca
     1081 ccttctgcca atgatcccgc cgccagttcc gccaaggctg ccgcgacggc aacgaagctg
     1141 gacgggagcg cagctttggg ttcggcggcc aatccgctgc gcgtgagcat cgtcagcttc
     1201 catggttacg cgccggcact ggtcgccaac ggcaacagcc tgaccacaca acaaaattcc
     1261 atttacagcg ccaagggggt caatgtccag tttgtcatac aggacgacat cccgacgctt
     1321 tcgaccattt ttgaatccgg tgcggcgcag tgtgcctggc gcacatccga cttctgggca
     1381 caggaacagc ccaacctgcg aaacgccgga ctcgatggtc gcgccgtcat gattgtcgac
     1441 aatacacggg gaggcgatgc cgtgattgcc cgcaacccgg ctatcgcctc aatcgaggat
     1501 ctggccggga gatcggtggc gctgctgcag ttcacgccat cgcacggcat gctgatcgac
     1561 gccattgaga attcttcgct ctctgcacga aagaaggaca gcatcaggat ggtctttatc
     1621 aatgccgagg aaggcacagc cggtgttcgc gccgctttcg agtccgccaa tgtcgaggcg
     1681 gccgtactat gggatcctga tctggcactg gcgcagcgcg ccgtaaaagg ttcgcgcatc
     1741 gtctattcga ccaaaacggc gaccaacctg atttttgacg tcatggtatg cgattcccgc
     1801 cttctcgcca aaccggaagg ccaggcggtc atccagaaat ttgtcgaggg ctggatggaa
     1861 ggcgtgcagg cggcgcgatc caatcccgac aatgcggtcg atgcactcgt caataccgag
     1921 gaatttttca agttgctggc agacaaggaa ggcaagccat tcatcaagag cttgttttcc
     1981 aatctggtgt ggacgggcgt cgaggataac gcccgcattc tcgggctggc tggtggcacg
     2041 aaccactacg agcgggtata ccggcgcttt gatgaaatct atcggaaggc gggcgcgctt
     2101 gcgaatccga agtcgccggt gattacgccg caagatagtt tcgactaccg tttcatcaag
     2161 tccatgctgg cccgtgacaa gtcagccagt gaggccgcgg cgaagcccca ggagacattt
     2221 tcccaagcgg cgttgaaaga cgcgacgcag aacgcggcga ttgtcaccaa gccggtcatg
     2281 gtcgggtttt cgaccggaac ggccgaactg accaagcggg cgcaaaaaac catcgacgag
     2341 gaaatggttc ctttcatcga aaataacggc aaggcgtatt tcgaggtcag cggcaattcc
     2401 gattccactg gctcacgtga agtcaatctg cggatttcgg gtgcacgcgc gaaagcggtg
     2461 gttgattatc tgaacgtgca gtgggagttc ccgcgtgatc gcttcaagat cgtcggcaac
     2521 ggtcctgacc ggccgctctg caacgagtcc aaccccgccg gcgaggggct atcgcttgag
     2581 gagtgccggg cgatgaaccg cactactcgc attggggtgt tcggcggtcg ctaggcgatg
     2641 gcggatttct ggaatcctta tgcatcgcct gaggcgaagc atgcacgcgt tctcggcttc
     2701 ggttcggtat tggcggtgct gattatctgg agtgtgctgt ccggctttgg cctggttggc
     2761 ccgacccggt taccggcgcc atgggatgtg ctaaaggcgt tctcctacct ggcatggtac
     2821 gatggcaaca gcatgttgct gacggcggct ctatggtcgg tcggcagact ggcggttgcc
     2881 ggcttttttg tcattctcat cggcattccc attggagtcg ccatgggcgc ctcgccgcgt
     2941 gtcaatgccg tcctttcacc gctggtcgat cctttcaggt cggcaccggt agtggcactg
     3001 ctacccatcc tggtaatgtg gctcggaatc ggcgaggcga tgaaggtggc gtttctcttc
     3061 atcggcgccg tcgtctatct gattccgatg gtgcgtgatg ccatccaggc ggttccgcaa
     3121 agttattgga tcggtgcgcg tgatctgggc gcaacgccct gggagtgcat tcagaaatcg
     3181 gtcattccga tggccatgcc acgtattgcg gatggcgtta ttgtcgcttt ctcggtcatg
     3241 tggacttaca tcacggttgc cgagtatgtg aacgcccggg tcgggctcgg gcaactgatc
     3301 cagaatgccc gccgtttcag cgcgtgggac caggtttttg ccggcatcat cgtcatcgtt
     3361 gggctggcat tgttgaccta ccagttgatg gtttttgcaa aacgacggct ctttccgtgg
     3421 gagacgcagc agtgatcgcg gccggcagcg ttaccgatgg cattggaaag aagccggaca
     3481 tcaccgttac cctcaagaat atcctgcagg aatacccggc accgggtggt ggtacaacac
     3541 gcgtgatcga tggcgtgagc gtcgattttt ccaaaccggg aatcaacatg ctgttggggc
     3601 catcgggttg cggcaagagc acactcctgc acatgatggg tggtgtccgg ccgatggggg
     3661 taaagacgcc gacctccggc acgatcctca tcgatggcgt ggaatgcgat ggtccgcacg
     3721 atgattcggt catggttttc cagcgttatg ccaatcgtcc ggatttgacc gtattcgaga
     3781 acgtcgcttt tccattccgg ctcaagctat ggaaaaagaa ggttccggat gccgagtggc
     3841 ggcagcgggt acaggaaatg ctgaaagccg ttggtttggc cgacaagcag aaactgcgac
     3901 cggcacaact gtccggtggg cagaatcagc gagtggctct ggcgcgggca ttggttctgc
     3961 gacctcgcat acttctaatg gacgaaccct ttggtgcgct ggacgcccag acgcgcgaag
     4021 aaatgcagcg cctgctcatt gaactctaca atacgtggcc ctgcctcgta atcttcgtca
     4081 cccacgacgt gaccgaagca ctgattctgg gcgaccgggt gatcgttctg tcctcgcaac
     4141 cggccaaggt tgccaacgat tttacgatcg atgtgccgcg cccgcgttcc actgactggt
     4201 tgcgttcgag ccaggcaacg caattggagg agggcatcct caaacaattg cacaaggcaa
     4261 gcctgggcag aggttcggtt cgggtaacgg tctgaagaga cactcgtggc agacaacgat
     4321 tcggcatacc tctctgaaat gctcaccagc cagggcaatc tctacgccct gctcggctcg
     4381 acagcgcttg gcgtggtctt gtccattccc tttggttttg gtgtcggcgc cattcccctg
     4441 attgcctttg ctgccggtga aatcatcgcg gcgatgtacc ttccgtcctc gatcgccttt
     4501 cgcgatcgcg tcgatcgcaa actgcgccgc caggcacggc tggcagcacg caagcagttg
     4561 ctgcaggaga tcgagtcacg ttgcgccaaa ggggcgattt catttcagca gctttcacga
     4621 acctacaccc ggatgtcgga gcgcatcgcc gccctttatt cacatgccga aagcggcagc
     4681 aagcgccttt cgctacagga cgtcgagcga ctggaggatg ccggtaccga ttacctgtgc
     4741 gtgtggctgg cggtgctggt aatggaggat cgggccgagc aaatcgatct ggccgatatc
     4801 gcagcgcgca ttgacgcgat cgatcgcgaa ctcaaatcac cccggccggg aagtgacatg
     4861 cgccagttgc aaaaggctcg cgccgagtac gtggccataa tcgagcgcta tcaccgcatg
     4921 tcgagtcgca aacgcgccct tgaagcagcg atggtatcga tgcctgacca gatggaagaa
     4981 atctaccaga ccatcatgac cacgtcggcc accgaggatg taggatcacg gctgggagat
     5041 gccattgcca aactgcggtt acaggaagac atcgaagccg aactggccga ggggagcagt
     5101 tttgtcagcg agcccaataa tgtcgtgcca ggcatcattg cacaggcgtc gcgatcggct
     5161 gcaagaaagc cgcaactggc cgcagtcaat cgtggccaga accactaatt catccagtaa
     5221 gaaaggaaac tcgcatggct gatatcaatt tcatcctacg cttatccaac ctgtggtccg
     5281 gattcgtctc cctgtggatt accgatgtgg aaaaacgtca cccggagatt gcgtaccaga
     5341 acgcgataga ttcaatgatc gagaagtatg gaaagctcaa gtccgccacc gcttccatca
     5401 tgcggcggcg cgaggacgtt tcggcacggc tgggcaggga tcagcaggaa cttgctgcca
     5461 tcaatgccga cctcaacgtc gccctggcta ccggacagga tgaactggga attgttctga
     5521 tccagaagaa aaatgccctg gaagccagca tcaagtcact cgaacaggac atggagcagg
     5581 cgcgtaccga tgccgacgaa gcaaaatcct cgctggtcca ggtgaagggc gagatccaga
     5641 aactgaagga agaaaaagac cgcatgttgg cgcagatgct cagtgccgaa gcgcgtctga
     5701 aaatccagaa ccagctcgaa gggctttcgg tcgatgccga agttcgggcc ttggacaatg
     5761 tccgcgagca tatcaagaat accgtggccg aagccaagat gggtagcgaa ctgcgcgatt
     5821 ctgatctgga tgttcagttg aacaagctgc gacagagcag tggcgcgatc actgctcgcc
     5881 agcaacttga ggagatgaaa cgcgtcaagg ccgcgcaagc cacaggcagc agcggcaaga
     5941 gcatgtgatg aatagcccgc cgaatgaagc gccgaaccgg catgaactgc cggcttgggc
     6001 tgaaaccgta cgtcagaaat acctggccgg cgaagcctcg gttttcgtcc tctacggaaa
     6061 tgttttcgac cggacattga tcaacgacaa gccttacgct ctggtggatt tcctgtcgga
     6121 gatcctgctc aaggacaaca aacagcgtgt tttcgaggtc agtattgatc gcggtgtgag
     6181 ggctctgcat caggcgagcc tggcagagaa ggaagaaatc taccggcaac tcgaaggcag
     6241 gggattggcc ggcattttcg agtcgctgga gcggcggatg cgggagcagc cctcaaacgc
     6301 cgtgatcttg ccgtatgccg gcacccttct gccggcggcg gaactgcatc tcctttcgct
     6361 ggaggagcgc ggtgcgttta ccaccctgca tcgctggtct ctcgatgatg acttgtccgc
     6421 caaggacaac gtgattattc tcatcaccga gtcgctggcg gaaatcaact ccggactgct
     6481 ggccaatccg cgcgtcgccg ctgtcgaact gccgataccc gagcgtgcgg cgcgcgaccg
     6541 ggccattcgc tactacgcac cgaaaatgcc ggacgagcag gttctggttc tggccgatca
     6601 gaccgccgga ctgcgcatca tccaactggc cagcatcgtt gccagcgaag ggccgcaagg
     6661 tctgaacgaa gggcagcgca gcaagctcat cggcgaactg ctgcagggta ctcccaatgc
     6721 cgcagagcgc tcggcgaagc tggcggcgat cacctccggc atgactccgg atgaaattcg
     6781 ccaactggtg gacccgacac gcccgttgcc tgatgcggaa gatccgtata gcgaggtgct
     6841 tgaggttgtg cgcaagcgca aacgcgaatt gatcgaaaag gaatgttcgg ggctcatcga
     6901 attcatcgaa gctcggcatg gtctggaggc agtcggtggc aacgatctga tcaaggcgga
     6961 gctgatggaa atcgcccacc tgatcaaggg cggcgagcgc ggccgggcac caatggggct
     7021 gctcgcggtt ggcgccatgg gttccggcaa gacatttgtc atcaaggctt ttctcaagga
     7081 ggccgggctg tccgggattg cgctcaagaa ttttcgttcc aagtgggtcg gctcgaccga
     7141 agccaatctt gaacgggtgt tggcaaccgt caaggccatg gggccgattg cactggtcat
     7201 tgacgaaggc gatcgcagct tcggcaacca gagtgaagac tcggacggtg ggaccagctc
     7261 gcgggtaatc gcccggctga aggagttcat gtccgacagc gataatcgtg ggcaggtcct
     7321 gtttatcctg atgaccaatc gccccgacaa gctggatacc gatatcaagc gccccggtcg
     7381 tctggatcgg aagatcccct ttttctatgc tgaaacaggt gccgagcggg cggccatcgt
     7441 agtggccatt ctgcgccgct atcgcgtcga atgtccgatt gccccgtcag cttggaatgc
     7501 gctgtgtgaa cagctcgaag gttattccaa cgccgatctg gaagcgctgg cgttgctggc
     7561 ggtcgagttc gccgggcgcg agggtggagt gctcagcgag acggtctttg cccgtgccgt
     7621 cgaagacttc atgccaccgc gtgaaaccac gatgctgagt tatatggaaa tgttggccgt
     7681 ctttgaaaca tcacgccgct cgctgttgcc accacgttac cgttcgctaa ccccgcagga
     7741 agtgcaggat caactgtccg ccttgcgccg ccaactcaat cactgaagga gtgtcttatg
     7801 tttcctctca agaccgatac cccgctgacg caagaccaag tggtgacagt cacccggctg
     7861 atgctgcacg tggcgcatgt ggatggcgac aagaccgcag aagagctgga gttgattcgc
     7921 gccttttacg aaggatgtgt agaagccggc aatggttggc agaccttcga tagtctggat
     7981 gggaagagcg gttcgccgga cgtcgccgca agcgattttg ccgaccaggg gcaacgcgaa
     8041 atggcacttg ccatctgtct gatggtcgct tgggccgatg gcgcattttc agccagggag
     8101 gacgaatcag ttcgcggcat tgccgacaag ctgggtgtcg gtggcgaacg tttcgcccag
     8161 gtgttggctc tggtcaagga tcacatgctt gcacagcttg ccggtttgcc ggatgtcggc
     8221 tcgattgttg cggtggcaaa ggaactggga tgaacaggga ggttgtatga agggaatatt
     8281 gggttttctg gcacgcgcaa atctggtgga gctaacagag gaagaaagac tgaaggcggt
     8341 cgctgacggc catgcagaaa cgccgggcag cgtcccgcca ccggaagagc cggttgcaca
     8401 ggcgccaccg ccgccccccc tgaatatcga agagtgcgaa attgccgatg accggccgct
     8461 ggaagaaata ttcagcgcgg ccggcgtccc cgagtcgcct tatccggtcg aaaagatgtt
     8521 gcgcctgctc gacggactgc gtgccatgga tgcagcgacg cgcaaggcgg cggttctggc
     8581 gatggatgcg gctgacgaca actggcagat tgccgactgt gtgggcgatg ccgagcgcaa
     8641 gatcgccgcg attgaagcgt acaaacaaca tctggcagct caggtagagg gaagcgagca
     8701 gcaggtgagc atgcagattg ctgaactcaa gtcggctctg gaaaatacga cggcagccat
     8761 ccgccggcaa atttcagagc tggaggaatt gctgcaacgg gaagttgcca aggaagccca
     8821 acagaccacc agcctggaag ccggtctgcg cgccacgcgt gaggcggcag ccagggaaac
     8881 gcggcggatg gctgccgagg tcgaacgtct gagcgagatc accctgcagt ttgggcctgg
     8941 atgagccggt aatcttggga ttttttgaac gtagccgacg ggtacagacc ggggtcatca
     9001 tgttgtccgt gacc
//
LOCUS       DAORPM010000082         8968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1859_length_8968_cov_6.248401, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000082 DAORPM010000000
VERSION     DAORPM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1859_length_8968_cov_6.248401"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLD53_05565"
     CDS             <1..396
                     /locus_tag="PLD53_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60412.1"
                     /translation="VPILCAIPSLFHELANSKLLHTLAPGAVDVSLGASELLPAMAIL
                     PFMLYQLAGFGTLHYVVSKQANWIINIGILGFIVMSTVANRNGNFTLEKALVSILVIS
                     MVIVVFYGILKLRQMQVTYDANCPQKEPK"
     gene            543..1187
                     /locus_tag="PLD53_05570"
     CDS             543..1187
                     /locus_tag="PLD53_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HRC60413.1"
                     /translation="MKANPKCVSRLLLSGLLAGLLCTALPAAAEWSWDPLKDAENVLP
                     EGAPNEGKIIQARHQVREMKEDALASLYEIAPSARRAIEHSAGYAVFSTFGIKLFFAG
                     GTTGKGMVVNRETGRQTFMKMVQVQGGLGFGVNKNRLVFVFTQEQALRNFIDQGWEFG
                     GQANLSAMADGQGGMFSGAASVAPGVFLYQLTETGLSATLTVSGTKFFKDADLN"
     gene            1319..2827
                     /locus_tag="PLD53_05575"
     CDS             1319..2827
                     /locus_tag="PLD53_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRC60414.1"
                     /translation="MTSLPLYRAADLRRIEAAAADQPLMQRAGYAAAKLAMMIGGDRG
                     AAILVLAGPGNNGGDAFEVARLLRERFFDPYTLFLGNAKGLPTDAAAAYQRFVEAGGI
                     THSALPAIFEGPRWSLIVDGLFGIGLKREIVEPYAGLIHNTNALAVRDGCPLLALDCP
                     SGLDADTGKLSGATILASHTITFIGGKPGLFTGDGPDHCGEVSVAPLDLDAQSIMPAA
                     GRSVGRELFSAQLKARPHNCHKGSHGDAGILGGAPTMVGAALLAARAALRLGSGRVYV
                     GLIDKHAPALDAQQLELMLRTPEKLIGSGLTALACGPGMGTSHEARVLLEKACALDLP
                     LVLDADALNLVAAEGELHVALATRNPSVAPTVLTPHPAEAARLLDCDVAVVQADRIGA
                     ALELSTRFNALVALKGCGTVVASPEDAWFINTSGNPGLATAGSGDVLTGMITALLAQG
                     WPALEALLAAVHLHGCAADCLVAQGSGPVGLTAGELIDSARGCLNAWISGAA"
     gene            2817..3740
                     /locus_tag="PLD53_05580"
     CDS             2817..3740
                     /locus_tag="PLD53_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC60415.1"
                     /translation="MQLRQHHAAHGVFLFLTAIFLFAALDATAKYLTAFFAVPLLVWA
                     RYFVHLVVMLITIAPGMGREIVVTRRPLLMTFRALMLVGVTLLVQLAFRTLPLAETTA
                     IVFVTPLLVALLAGPMLGEKMQLKGWLATLAGFCGVLLIARPGGAMVGIGVIYALGGA
                     LCYALYQILTRKLLASEPPMRLLFHTALVGTLSMSLFMPEYWGGELPGLWHILLISSL
                     GFYGGIGHFLLIRAFREAPASTLSPLLYVQLIWAMLLGYAVFGQLPDMPAIMGMLIIG
                     ASGLSLVMWRTPALIPAMHTESALEKQPVSE"
     gene            3831..4112
                     /locus_tag="PLD53_05585"
     CDS             3831..4112
                     /locus_tag="PLD53_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60416.1"
                     /translation="MKHIIWVLWPAFIAAGIAEIVFFTAIDPQQLYLLGQPVELPAMA
                     TYSIGFLMFWLICAGSSLMTWFMLPHEVKSALERYAGGRDDLARPKRTR"
     gene            complement(4151..7168)
                     /locus_tag="PLD53_05590"
     CDS             complement(4151..7168)
                     /locus_tag="PLD53_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRC60417.1"
                     /translation="MQLIVLRLVLSCLLMFTGSVHADDEADDFFARDSTAAAALEKQI
                     AMAPPDDNSPQELCVFLHKRGDAYRRLGRYQQSLTDLRLALDMKQAASPELWCDRWRL
                     QGDIHGSIYSSGDWLQLADYALTVSDEYKNSNKWHYFSSLLWNVDAQLNLARLRQAEE
                     SFQRAGQLIPALRQSRAWATYGANQMGRYSSYAAKMQALRGNLVESERFRRQALDYAQ
                     QFLDATSANRPADHLDNRLAANALIQRKRQLAGILSAQGKTGEAEILARQALQQTLAR
                     SGRNTLAVSASYRMLGHVKLQQGQIDEALRLQERALDTLLHSDTRSYSTALSDQRAQI
                     GFLLGVQGRWAEALTVYEERDQGLRSNAAQFASSGSNNITWAMALLKRQRAPEAEKML
                     RAIINWNLRKPFVDPLYLAYLHGYLGVVLVDAGKNDDALAEFREAFPVLIRQADSDNS
                     SENGGFVRPYRLRLIADGYLELLSRMSAARTEQTGFDPAAEAFQVAEVARGSSVQLAI
                     ASSAARASLPDAALADLARREQDTANQISALNNLLIRLASESESNRLDKTIADIRKDV
                     SQLDAQRTQLRKELAERFPAYAELVLPKPPTPASVQKVLHPNEAAVAFYVAEKQTYVW
                     TITPTRTAFRAVPMTRQQVDQHIAALRRAFDLTGAALIPFDGAAASSLYAALLAPDDA
                     LWSDVRILNVVPHGSLGQLPFAVLLTSGTADANASNLADRPWLTKKVALAQQPSAGTL
                     IALRGQGRIAAQRQAFVGFGDPLFHTQTGRHATGTRSVRNLSVLPSRTPEALPDTDGS
                     ALLRGFAQLPLLPDTAEELNDIGKTLGANPKSDIFLGKKATEQNVKSSDLAAYRVVAF
                     ATHGLIPGELRGLDQPSLAMANPALTDDKNNDGFLTMSEVLGIKLDADWVVLSACNTA
                     SGDGKNEEAVSGLGRAFFFAGSRRLLVSYWPVETVSARLLTTELFKRLSGKPDESKAE
                     ALRHSMLQLMAMSKEYGHPAFWAPFGLIGEAGK"
     gene            complement(7357..8142)
                     /locus_tag="PLD53_05595"
     CDS             complement(7357..8142)
                     /locus_tag="PLD53_05595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC60418.1"
                     /translation="MLNLSKLAAALLFGAMISASAVAAGKAFVTVNGVAVSQTLADAL
                     MAEQKAKGAPDSPELKNAVREELIRRELVAQEAKKAGLEKKPDIAVQADAARQGVFIR
                     AYIIEYVKKNPISDAELKAEYEAIKAKLGNTEYKARHILVKNEEDAKVIIVNLKKGAK
                     FEELAKQSIDPGSKDNGGDLGWASAGNFVKPFSDALTSLSKGKYTEAPVKSEFGYHVI
                     LLEDSRPLNVPPFDEIKPRLAQQAQAQQINKMVDGLRAKAKIE"
     gene            complement(8216..8512)
                     /locus_tag="PLD53_05600"
     CDS             complement(8216..8512)
                     /locus_tag="PLD53_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRC60419.1"
                     /translation="MSETNPDRASDGFESVLRERLAGLKPLRLELIDDSAKHAGHQGA
                     KGGGGHYRLLIVSDAFSGKPTLSRHRLVYDALGELMRSKIHALSIQSIAPEEAQ"
     gene            complement(8509..8811)
                     /locus_tag="PLD53_05605"
     CDS             complement(8509..8811)
                     /locus_tag="PLD53_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRC60420.1"
                     /translation="MLYAIVGEDRPDSLTARLQARPAHVERLKVLQEQGRLILAGPFP
                     AIDSPDPGPAGFSGSLIVAEFTSLAAAQTWADADPYMASGVYVRVTVKPFNKALPA"
     gene            complement(8811..>8968)
                     /locus_tag="PLD53_05610"
     CDS             complement(8811..>8968)
                     /locus_tag="PLD53_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HRC60421.1"
                     /translation="FMGFANLAVAFAFNFSTDDWVNFKLFGGVGLMLVFVIAQGLVLS
                     KYIEEEK"
BASE COUNT         1686 a         2615 c         2692 g         1975 t
ORIGIN      
        1 gtgccgatcc tgtgtgccat accttccttg ttccatgaat tggcgaacag caagttgcta
       61 catacgcttg cccccggcgc agtcgacgtt tcactgggcg cctctgagtt gcttccggca
      121 atggcgattc tgccgttcat gctttatcag cttgccggct tcggcaccct gcactatgtt
      181 gtgtcaaagc aggccaactg gattatcaat atcggcattc tcggttttat cgtcatgagc
      241 acagtcgcaa accgcaacgg gaacttcacc ctggaaaaag ctctggtttc gatattggtc
      301 atttccatgg taatcgtcgt tttctacggc atactcaaac tgcgccagat gcaggtgacc
      361 tacgacgcaa actgcccgca gaaggaacca aagtagttga gaccgacaag ccgtgcatgc
      421 tgtgttgatt acttggccgg attctgcagc gatagccgac agaagcggaa tttggcgata
      481 gatcgagttt agaatggctg aagttgtttt tttagatgca ctactttttt gagagccaag
      541 aaatgaaagc caacccgaaa tgcgtgtcgc gtctgctgtt gagcggactg cttgccggac
      601 tcctttgcac ggctttgcca gcggctgccg agtggtcctg ggatccgttg aaggatgctg
      661 aaaatgttct gcccgaaggt gctccaaacg aaggaaagat tatccaggcc cgccatcagg
      721 ttcgtgaaat gaaagaggac gcgcttgctt ccctgtacga aatcgcgccg agcgcgcgac
      781 gcgccattga gcattcggcg ggttatgccg tgttcagtac cttcggcatc aagctctttt
      841 ttgccggcgg cacgacgggt aaagggatgg ttgtcaaccg ggaaaccggt cgccagacct
      901 tcatgaagat ggttcaggtg caaggggggc tgggattcgg tgtgaacaag aatcgccttg
      961 tatttgtttt tacccaagaa caggcactgc gtaatttcat cgaccagggc tgggaatttg
     1021 gtggacaagc caatctttcc gctatggccg atggtcaggg cggtatgttt tccggtgccg
     1081 cttcggtcgc gccaggcgtt tttctctacc aactgacgga gaccgggctc tccgccacac
     1141 tcaccgtgtc gggtacaaaa ttcttcaagg acgccgatct caactaagtg accgcggcgt
     1201 gtcctgagat attttgcgag cgctggcccc gttcctgcag cccccggatc acggaatacc
     1261 ggaaaatccg ttatccgtta tccggtatgc catcgtgatg taacgagggg cgtcgaccat
     1321 gacttcacta ccgctctacc gcgctgctga tcttcgccgc atcgaagccg cagcggcaga
     1381 ccagccgttg atgcaacggg caggttatgc ggcagccaag ctcgccatga tgattggtgg
     1441 tgatcgcggc gcggcgatcc ttgtcctcgc gggccccggc aataacggtg gcgatgcttt
     1501 tgaagtagcg cgtctgttgc gtgagcgctt tttcgaccca tacacgctct ttctcggcaa
     1561 cgcaaagggt ctgccgacgg acgcagcggc agcttaccag cgctttgtcg aagcgggcgg
     1621 gattactcat tctgcattgc cggcgatctt cgaaggcccg cgctggtcgc tcatcgtcga
     1681 cggtctgttc ggtatcggcc tcaaacgcga aatcgtcgag ccttatgccg gtctcatcca
     1741 taacaccaac gcgctggctg tccgtgacgg ctgcccgctg ctggcgcttg actgcccgag
     1801 cggcctcgac gccgacaccg ggaagctaag cggcgcaaca atccttgcca gtcacacgat
     1861 taccttcatt ggcggcaagc ccggactgtt caccggcgac ggccccgatc attgcggaga
     1921 agtgtcggtc gctccactcg acctcgatgc gcagagcatc atgccggcag cagggcgcag
     1981 cgtaggccgc gaattgtttt cggcgcagtt gaaagcgcgc ccgcataact gtcacaaggg
     2041 cagccatggc gacgccggca ttctcggcgg ggcaccgaca atggtcggtg ccgcgttgct
     2101 cgctgcccgc gcggcgctac gcctgggcag tggccgggtc tatgtcggcc tgatcgacaa
     2161 gcatgcgccg gcgcttgatg cgcagcagct cgaactgatg ctgcgcacgc ctgagaaatt
     2221 gattggcagc gggctcacgg cgctggcctg cgggccgggc atgggtacca gccacgaggc
     2281 gcgcgtgttg ctcgaaaagg cttgcgcact ggatctgccg ctagtgctcg acgccgatgc
     2341 gctcaacctc gttgccgcgg agggcgagct gcacgtggcg ctggctacgc gcaacccgtc
     2401 tgtcgcgccg accgtgctta cgccgcaccc ggccgaagcg gcgcgcttgc tcgactgcga
     2461 cgtggccgtc gttcaggccg accgcatcgg cgccgcgctc gaactgtcca ctcgattcaa
     2521 cgcactggtc gcgctcaagg gctgcggcac tgtcgtggcc tcgcctgaag acgcatggtt
     2581 catcaacacc agcggcaatc cgggactggc tacagcgggc agcggcgatg tgctcaccgg
     2641 catgatcacc gccttgctcg cccagggctg gccagcattg gaagcactgc tggctgccgt
     2701 gcatctgcac ggttgcgccg ccgactgtct tgtcgcccag ggctccggcc cggtcggtct
     2761 aaccgccggc gaattgatcg acagcgcgcg tggctgtctc aacgcatgga tctctggtgc
     2821 agcttaggca acatcacgcc gcacacggcg tcttcctgtt tctcacggcc atctttcttt
     2881 tcgccgcact cgatgccaca gcgaagtacc tgaccgcttt ttttgccgta ccactgctcg
     2941 tctgggcgcg gtatttcgtt catctggtcg tcatgctgat aaccattgca cccggcatgg
     3001 gacgcgagat agtagtcacg cgacgcccac tgttgatgac ttttcgcgcg ctcatgctgg
     3061 ttggtgtgac tctgcttgtg cagcttgcgt ttcgcacctt gccgctggcc gaaacgaccg
     3121 cgattgtatt cgtcacgccg ctgctcgttg cgttgctggc aggcccgatg ctcggcgaga
     3181 agatgcagtt gaaaggctgg ctggcaacgt tggcgggatt ttgcggcgtg ctgctgatcg
     3241 cccggccggg cggcgcaatg gtcggcatcg gtgtcatcta cgcgctcggc ggcgcgcttt
     3301 gctacgccct ctaccagatt ctcacccgca aactgttggc tagcgaacct ccgatgcggc
     3361 tgctcttcca tacggcgctg gtcggcacgc tttcgatgtc gttgttcatg ccggaatact
     3421 ggggaggtga actgccgggc ctgtggcata tcctgctcat ttcctcgctc gggttctacg
     3481 gcggcatcgg ccattttctg ctgatccgtg ccttccgcga ggcacccgcc tcgacgcttt
     3541 cgccgctgct ctacgtccag ttgatctggg ccatgctgct cggctatgct gtttttggcc
     3601 agttgcccga catgccggcg atcatgggca tgctgatcat cggcgcgtcg ggactgagtc
     3661 tggtgatgtg gcggacaccg gcactcatcc cggcaatgca caccgaaagc gcgctggaaa
     3721 aacagccggt aagcgaatag acaggctgac aaggcaggga gaaccttttt gctgtcggca
     3781 actcgaagaa cgttgtaaca ttttggtggc tttatttttt ggagcgcgca atgaaacaca
     3841 ttatctgggt actctggccg gcatttatcg cggccggaat tgcagagatc gttttcttta
     3901 cggcaatcga tccgcagcaa ctctatttgc tcggccagcc ggttgaactg ccggcgatgg
     3961 cgacctactc gatcggcttt ctgatgttct ggctaatctg tgccgggtcc agcctgatga
     4021 cctggttcat gctgccgcat gaagtcaaga gtgccctgga gcgctatgct ggcggccgcg
     4081 acgatctggc tcgcccgaag cgtacccgct gaccgacaga cacgtctgac gaagcgtgtt
     4141 gctcagcttc ctacttcccg gcctctccga tcaagccgaa cggcgcccag aaggccggat
     4201 gaccatactc tttggacatt gccatcagtt gcagcatcga atggcgtagc gcttcagcct
     4261 ttgattcgtc cggttttccg ctgaggcgct tgaacagttc ggtagtcagc aatcgcgccg
     4321 aaaccgtttc gaccggccag taggagacca gcaggcggcg cgaacccgcg aagaaaaagg
     4381 cccgcccgag gccggatacc gcttcctcat ttttgccgtc gccgctggcc gtgttgcacg
     4441 ccgagagaac gacccagtcg gcatcaagct tgatgccgag cacctcgctc atggtcagga
     4501 agccgtcgtt gttcttgtca tcggttagcg ccgggttggc catcgccaga ctgggctgat
     4561 cgagtccgcg cagttcgcca ggaatcaggc cgtgggtcgc gaatgcgacg acgcgatacg
     4621 ccgcgagatc actgctcttg acgttttgtt cggtcgcctt cttgccaaga aaaatatcag
     4681 acttcgggtt ggcacccagg gtcttgccga tatcgttcag ttcttcggcc gtgtcgggca
     4741 gcaggggcag ttgagcaaag ccgcgaagga gtgccgaacc atcagtatcg gggagcgctt
     4801 ccggggtgcg cgacggtaag accgagagat tgcgcaccga acgggttccg gtggcatgcc
     4861 tgcccgtttg tgtgtggaat agcgggtcgc cgaatccgac gaaggcttgg cgttgtgccg
     4921 cgatacgccc ctggccgcgc agtgcgatca gcgttcctgc cgaaggctgc tgtgccagcg
     4981 ccaccttctt cgtcagccag ggtcgatcgg ccagattgct ggcgtttgca tcggcagttc
     5041 ccgacgtcag aagaaccgcg aacgggagct gcccgagcga accgtgcgga accacgttca
     5101 gtatccggac atcggaccac aaggcgtcat ccggtgccag cagcgctgcg tagaggctgc
     5161 tggccgcggc cccgtcgaac ggaatcaggg cagcaccggt caggtcgaac gcgcggcgca
     5221 aagcggctat gtgttgatcg acctgctgac gcgtcatggg aactgcgcgg aatgctgtgc
     5281 gcgttggggt gattgtccag acgtaagttt gtttttcggc gacgtagaag gcgacggcgg
     5341 cttcgttggg gtgcagcacc ttctggacac tcgctggcgt tggcggtttc ggcaaaacca
     5401 actcggcata ggctggaaac cgttcggcca gttccttgcg caattgagtc cgctgagcgt
     5461 ccagctggct cacgtcttta cggatgtcgg caatggtctt gtcgaggcga ttcgactcgg
     5521 actcggatgc gaggcgaatc agcagattat tcaatgcact gatctgattg gccgtgtcct
     5581 gctcgcgtcg cgccagatcg gccagcgccg catcgggcag cgacgcacgg gcggcactgc
     5641 tggcaatcgc cagttgaacg ctcgatccac gtgctacttc ggcaacctga aacgcttcgg
     5701 cagccggatc gaaaccggtc tgctccgttc tcgcggcgga catccgggac agcagttcca
     5761 gatagccatc ggcaatcagt cgcaaccggt aggggcgtac gaagccgcca ttctccgagc
     5821 tattgtcgct gtcggcctgg cgaatcaaaa ccggaaacgc ttcgcggaac tcggccaggg
     5881 cgtcatcgtt cttgccagca tcgaccagaa ccacgccaag atagccgtgc aggtaggcca
     5941 gatagagcgg gtcgacgaag ggtttccgga gattccagtt gatgatcgca cgtagcatct
     6001 tctcggcctc tggagcgcgc tggcgcttga gcagggccat tgcccaggtg atgttgttgg
     6061 atcccgaact ggcaaactgc gcggcgttgc ttctcaaccc ctggtcgcgt tcttcataga
     6121 ccgtcaacgc ctccgcccag cggccctgga caccgagcag gaagccgatt tgagcgcgct
     6181 ggtcggataa cgccgtggaa taggagcgcg tgtctgaatg caagagcgta tcgagcgccc
     6241 gttcctgcag acgcaacgct tcgtcaatct gaccctgctg cagtttgacg tgcccgagca
     6301 tgcggtacga agccgacacc gccagcgtat tcctgcctga tcgcgcgagg gtttgctgaa
     6361 gcgcctgacg cgcaagtatc tcggcttcac cggttttgcc ttgtgcggag agtatgccgg
     6421 cgagttggcg cttgcgttgg atcaacgcgt tagccgccaa gcggttgtcg aggtgatcgg
     6481 ccggacgatt ggccgaagtc gcgtcgagga attgctgggc gtagtccagc gcctgcctgc
     6541 ggaacctttc ggattccact aggttgccgc gtagggcctg catctttgcc gcataagagc
     6601 tgtagcggcc catctggttg gcgccgtagg tggcccacgc ccggctttga cgcaaggccg
     6661 ggatcaattg cccggcacgc tgaaaggatt cttctgcctg gcgcagtctg gccaggttca
     6721 attgcgcatc gacattccat agcagactcg aaaagtagtg ccatttgttg ctgttcttgt
     6781 attcgtcgct gacggtcagt gcgtaatcag cgagttgcag ccagtctccg gaagaatata
     6841 ttgagccatg aatatcaccc tgcagtctcc atcggtcgca ccacagttcc ggcgaagccg
     6901 cttgcttcat gtccaatgcc agcctcagat cggtcagcga ttgttggtag cggcccaacc
     6961 gtcgataggc atcaccgcgc ttgtgcagga atacgcacaa ctcctgcggg gaattgtcgt
     7021 caggcggcgc catcgcaatc tgcttttcca acgcagcggc agccgtggag tcgcgggcga
     7081 aaaaatcatc cgcctcatcg tcggcgtgca cgctgcctgt aaacatcaga aggcagctaa
     7141 gaacaagtcg gaggacgatc agttgcatgg ctgcctgcgg ttgagaaatg ttcgaggttt
     7201 gatcatcgaa tggtcaagga atagcgctgg ctcattgtca ccccaagttg atttgatgcc
     7261 aagtcaattt ccaggcgaag gagcgacttt gcggaggcgc aaaaaaacgc cgggaggctt
     7321 gcggcctcac cggcgttcag ttttcgaccc tgagggttac tcgatcttgg cctttgcacg
     7381 caggccgtcg accatcttgt tgatctgttg tgcctgcgct tgctgggcca ggcgcggctt
     7441 gatttcgtca aaaggcggta cattcaaggg gcggctgtct tcaagcagga tgacatggta
     7501 gccaaactcc gacttgaccg gggcttcggt gtatttgccc ttgctcagac tggtaagtgc
     7561 atcgctgaac ggcttgacga agttgccggc actggcccag cccagatcgc cgccattgtc
     7621 tttggagccg gggtcaatcg actgcttggc aagttcttcg aacttggcgc ccttcttgag
     7681 attgacaatg atgactttgg cgtcctcttc gttcttgacc aggatgtggc gagccttgta
     7741 ctcggtgttg ccgagcttgg ccttgatcgc ctcgtattcc gccttcaact ctgcgtcact
     7801 gatcgggttt ttcttcacgt attcgatgat ataggcacgg atgaaaacac cctgacgcgc
     7861 ggcgtcggcc tgtacggcga tgtccggttt cttctcgaga ccagccttct tcgcctcttg
     7921 ggcaaccagc tcgcgtcgga tcagttcttc acgcaccgca ttcttcagtt ccggcgagtc
     7981 gggagcgccc tttgctttct gttcggccat cagcgcatcg gccagagtct gtgaaacggc
     8041 aactccgttg accgtgacaa aggctttgcc ggcggctacc gccgatgccg atatcattgc
     8101 gccaaacagc agggcagccg caagtttcga gagattcaac atgtgtcgta ttccttctgt
     8161 cagatttgaa tcgggtagga tagggtttct tgttataaga gtaaacgggg tgatcttatt
     8221 gtgcctcttc cggagcaatg gactggatgc tcagcgcatg aattttacta cgcatcagtt
     8281 cgcccagggc gtcatatacg agccgatgcc ggctcagggt cggttttccg gagaacgcgt
     8341 ccgagacaat gagcagtcgg tagtgcccac cgccaccttt ggcgccctga tgcccggcat
     8401 gcttcgccga atcatcgatc agttccaggc gaagcggctt taatccggcc agacgttcgc
     8461 gcagaacgga ttcaaaaccg tcagaagctc tatccggatt ggtttcgctc acgcgggcag
     8521 tgccttgttg aagggtttga ccgttacccg gacgtataca ccgctggcca tatagggatc
     8581 ggcatcggcc caggtctggg cggcagccag cgaggtgaac tcagcaacaa tcagactccc
     8641 ggaaaaacct gccgggccgg ggtccggcga gtcaatggca ggaaatgggc ccgccagaat
     8701 cagccggccc tgttcttgca gcactttcag gcgctcaaca tgcgccggcc gggcctgaag
     8761 tcgggcagtg agtgaatccg gccggtcttc accgacaatg gcatagagca ttatttttcc
     8821 tcctcaatgt actttgacaa aaccagtccc tgggcgatga cgaaaacgag catcagcccg
     8881 acaccgccga aaagcttgaa gttgacccag tcgtcagtcg aaaaattgaa ggcgaatgcg
     8941 accgcgagat tggcgaatcc cataaata
//
LOCUS       DAORPM010000083         8940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1871_length_8940_cov_11.751604, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000083 DAORPM010000000
VERSION     DAORPM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1871_length_8940_cov_11.751604"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..4448
                     /locus_tag="PLD53_05615"
     CDS             375..4448
                     /locus_tag="PLD53_05615"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HRC60422.1"
                     /translation="MSKPVCATVLLFALYLIFGFFALPAIVKWQIEKQVPEKLGHPIS
                     VGEVRCNPLLLTFEVGDLVLSNTEGSPILSFKRLQIDFAMRSVVDQAWTFSLASLEAP
                     VARLNFDKEGRHSFSALLERLIADEPESQDSPLPRFVVSRLSLSDGRLEISDEFLDEP
                     LVAHIEPLSVDISNLSSLHAQPAHYRLSVRTRLGETLDASGELTLDPFASNGEVALGN
                     LKAVTLAASLSRLFRLDSLAGNISAAASYDLALDQNGAIAGTVHDVALEIAEFSLSAP
                     GVPSPLLAFEAFALKQGRVDLAAHQISLASVSLTKGRVAAVQDDHGNVDWVRLVLPAS
                     AAAAFEPPPTNAVSESAHKEANATATTLAKPWRISATDAEVSEMALSFSDAANKLKVQ
                     LASVSLDTAPSAEFGPAGLSFVLNHPRLTISGAQLKSGSDSLGVSEVVIEAGQTRFVA
                     ADTQLKLGIDEPHSTLSAVQLQLGGDTMEIGRSEIASDTLSLTQDGGDTRIEATVVRT
                     ALSNFQAQRNTNRINVRDASFATRSISFANGVDARFDDVTLKLKSADVAALDPPAGTG
                     GASAADFGAKSLRIAFADGPLDVTGDGLSASLSDAVMRSPDDASEMLRLSSVALSGGV
                     FKLEDRLMTAESLVLANGKTRIGLDAQGNFAGLNVSRKALEMADPDAQPDTARTTAQS
                     RQEQARAWRLALKSAKAADIAVGFEDHRLTPPMTLGLNVLRGEAKDFSTSSASPMSLD
                     IKARLDSGGDIAASGNVHADGGTSDLKISLAGVELVPAQAYLSEFAKLKLVSGTLSGS
                     GRLRYNNPTGSRLAYEGSVALDRLLLEEIQPKRTFLSWDSVASDDIVVTLEPNRLDIG
                     ELRVDRPSGRLIIAADQSVNLMDVLRQPNKNDATEEQKAIQVSGDKAPADEQQDDIQA
                     QIVERDLFPFTIAFVRVSGGTLEFADQSLRPQFGTRMHELKGVITGIGSDPNHGAKVQ
                     LDARVDKYGSAKIRGQISILKPERFTEIDMTFRNLEMTSLSPYVAKFAGYSIASGRLA
                     MDLQYRVKSGHLQGENKIVLKQVELGEKVESPQALDLPLELALAILKDSRGVIDVELP
                     VSGDLNDPQFDYGAVIGKAIGNLLGSVILAPFKALGALFGSGDEKLDSIDFEPGSADI
                     APPERQKLGTVARALKARPSLTLIVPPTYAANMDTVALKSRAVRSEIVQRMGIELIRG
                     EDPGPIDTSNPRVQHAIEEMFIQHYAPEVLSTLKRKALQSETMIGDPYVPAGESGPRD
                     ATQTGKPMSDPLPAFYQGLVDRLITEEAVSEEMLLQLAARRSDAIIAELVLTGGIPAA
                     RIVPGPLDKSAQANTMNESNGVNERAVTLRLQLEVSK"
     gene            4445..5815
                     /locus_tag="PLD53_05620"
     CDS             4445..5815
                     /locus_tag="PLD53_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRC60423.1"
                     /translation="MKQNAKALYGRFEKSVWGVAETPLPLWKERAQRGLRTVFILTRD
                     LVGGQLTLRAMSLVYTTLLSIVPLLALSFSVLKAFGVYNQIEPILLKFVEPLGEKGDE
                     IVHQISIFIEKMNVGVLGALGLTLLLYTAISLMQKIEESLNFIWHIPHPRPLADRFSR
                     YLSVLLVGPILVFSALGITASVMNIETVRDLMEINILGQAMQAISRLTPYFLVTAAFT
                     FIYLFIPNSRVRVVPALIGGVAGGFAWQTAGWVFAVFIASSNKYAAIYSSLAILILFM
                     IWLYLSWLILLFGASVAFYAQHPEYLYARSGEPRLSNRMRERLALSVMSLVSGRFLAG
                     EPMPSLAEFTRLLGVPMHSLQIVLVALEGNRFLAQSSDEPPRFLPSRDPSLILVTQVL
                     ETVRAAGEEQFLSPEGLPAPPPVDALLNQIQQAVESSVSNMTMRDLALQISGRALHEP
                     VFPKDS"
     assembly_gap    5914..6013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6014..6335)
                     /locus_tag="PLD53_05625"
     CDS             complement(<6014..6335)
                     /locus_tag="PLD53_05625"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRC60424.1"
                     /translation="MARPLPELVEHACELFAPLGAIRVKAMFGGYGFYCDELFFAIFA
                     YETLYLKADAESTEAFRQAGGEAFHFTYKDGRTETMNYWTVPEEAMESSAEMLPWGRL
                     ALEAA"
     gene            complement(6343..7176)
                     /gene="metF"
                     /locus_tag="PLD53_05630"
     CDS             complement(6343..7176)
                     /gene="metF"
                     /locus_tag="PLD53_05630"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRC60425.1"
                     /translation="MKRTELSIEFFPPQTPEGVDKLRIVRGKLAVLKPEFFSVTFGAG
                     GSTRDRTFSVIKEIAVEGFEAAPHLSCIGSTRENIRDILHAYKSAKIRRIVALRGDLP
                     SGMAEAGEFRYANELVEFIRSETGDHFRIEVAAYPEWHPQARSPRDDLAAFARKAGAG
                     ANSAITQYFYNADAYFHFVDEARALGVVIPIVPGIMPIANFSKLARFSDACGAELPRW
                     MRRKFEAFGDDAESIRAFGLDVVTELCERLLTGGAPGLHFYSMNQSVLTLEICRRLKL
                     G"
     gene            complement(7200..7550)
                     /locus_tag="PLD53_05635"
     CDS             complement(7200..7550)
                     /locus_tag="PLD53_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRC60426.1"
                     /translation="MRLILLWILNAVALLAVAWLLPNSIHVASFGSALLAALILGLVN
                     ALVRPVLLVLTLPVTLLTLGLFIFVLNGLMFWLAGSLIDGFVVAGFWPAVLGSMVYSV
                     VSWALSGLLVSGKN"
     gene            complement(7577..>8940)
                     /gene="ahcY"
                     /locus_tag="PLD53_05640"
     CDS             complement(7577..>8940)
                     /gene="ahcY"
                     /locus_tag="PLD53_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRC60427.1"
                     /translation="LADWGRKEIRIAETEMPGLMAIREEFAAAQPLKGARITGSLHMT
                     IQTAVLIETLTALGANVRWASCNIFSTQDHAAAAIAAVGVPVFAVKGESLSDYWDYTH
                     RIFEWGDAAYSNMILDDGGDATLLLHLGARAEKDISVLAKPTSEEETILFAAIKAKLS
                     TDPTWYSVRLAAVKGVTEETTTGVHRLYQMHERGDLKFPAINVNDSVTKSKFDNLYGC
                     RESLVDGIKRATDVMIAGKVALIAGYGDVGKGSAQAMRALSAQVWVTEIDPICALQAA
                     MEGYRVVTMEYAADKADIFVTCTGNYHVITHEHMAAMKDQAIVCNIGHFDNEIDVASV
                     EKYTWEEIKPQVDHIIFPDGKRIILLAKGRLVNLGCGTGHPSYVMSSSFANQTIAQIE
                     LFTRNADYPVGVYTLPKHLDEKVARLQLKKLNAQLSVLTDQQAAYIGVPKEGPYKAEQ
                     YRY"
BASE COUNT         1866 a         2565 c         2587 g         1822 t
ORIGIN      
        1 ttgaggcgct ggttgattcg aaactgaccg cctgaatgcc cgtgtcggat cgatctgtct
       61 cgcgcaccgg aacgcctcaa ggattgtgtt gtcgatacca atccactaca gaaatcaagc
      121 gtaggctgcc gctacgttag ctgcgtccat gcgaccccac atcgagcaac acgaagatat
      181 cctcggcgcc tagtggggcg tcgccgaaat cggccaaacc agtcccgcgt cttgggctga
      241 aactttggct ttccaacgtt caaagctagc gcgaagacct tcgccttgtt gcacgctgac
      301 cacaaacatg caggatcggc caacctccgt accgtgaaac cccaactatg cctgacagga
      361 tgcgaagagt tcttttgagc aaaccggtgt gcgccaccgt gctgctgttc gcgctctatc
      421 tgatctttgg cttcttcgcc ttgccggcaa tcgtcaagtg gcagatcgag aaacaggtgc
      481 ccgaaaaact tggacacccg ataagcgtcg gcgaggtacg ctgcaatccc ctactgttga
      541 cgttcgaagt cggcgatctc gttctctcta acaccgaagg ttcaccaata ctgagcttca
      601 agcgcttgca gatcgatttc gctatgcgca gcgtcgtcga tcaagcgtgg accttttcgc
      661 tcgcctctct tgaggcaccg gtcgcgcgcc tgaatttcga caaggaaggg cgacatagct
      721 tcagcgcact gctcgaacga ctgatcgccg acgaaccgga atcgcaggat tcgcctttac
      781 cgcgatttgt ggtatccagg ctttcgctga gcgatggtcg ccttgagatc tcggatgaat
      841 ttctcgacga gccgctggtg gcacacatcg aacctctttc ggtagatatc agcaatcttt
      901 cgagccttca cgctcagccg gcacactatc ggttatcggt gcgaacacgc cttggtgaaa
      961 cactcgacgc gagtggcgag ctgacgctgg acccgttcgc ttcgaatggg gaagttgcgc
     1021 taggcaacct caaggcggta acgctggctg caagcctttc gcgtctgttc aggctcgatt
     1081 ccctcgcagg caatatcagc gccgccgcca gctacgattt ggcgcttgat cagaacgggg
     1141 cgattgccgg aaccgtacat gatgttgctc tcgaaatcgc cgaattttcc ttgagtgccc
     1201 ctggcgtgcc ctcaccactg ttggcattcg aggcattcgc cctgaagcaa ggacgagtcg
     1261 acctggctgc acaccaaatc agcttggcga gcgtcagctt aacgaagggc cgtgtcgccg
     1321 ctgtgcagga cgaccatggc aacgtggact gggtaagact ggtgctcccg gcttcggctg
     1381 ctgcggcgtt tgaaccgccc ccgacgaatg ccgtctccga atcagcccac aaggaggcaa
     1441 acgcaacggc cacaactctc gcaaaaccgt ggcgaatctc ggcaaccgac gcagaggtgt
     1501 cggagatggc attgagcttc tccgacgcgg caaacaagct gaaagtgcag cttgcaagtg
     1561 tgtctctgga tacggccccg agcgctgaat tcggcccggc cggattgagt ttcgtgctga
     1621 accacccgcg gctcacgatc tccggggcgc aactgaaaag cggttcggac tcgttgggcg
     1681 tctctgaagt ggtcatcgaa gccggccaga cccggttcgt tgctgcagat actcagctca
     1741 aactcgggat tgatgagccg cacagcactt tgagcgctgt ccagctccag cttggcggtg
     1801 acactatgga aattggccgc tcggagatcg ccagcgacac gctgtcactg actcaggacg
     1861 gcggtgacac caggatcgag gccactgttg tgcgaacggc actctcgaat ttccaggccc
     1921 agagaaacac caatcggatc aacgtccgcg acgcatcatt cgcaacacgt tcaatcagct
     1981 ttgcaaacgg tgttgatgcg cgtttcgacg atgtaacgtt gaaactcaag tcggccgatg
     2041 tcgcggcgct cgaccccccg gccgggaccg gtggcgcgtc agcggcggat tttggcgcca
     2101 aatccctccg cattgcattc gctgacgggc cgctcgatgt tactggcgac ggtctgagcg
     2161 caagcttgtc ggacgccgtg atgcgcagtc ccgatgatgc aagcgaaatg ctgcgcttga
     2221 gtagtgtggc gctgagtgga ggcgttttca aactggagga tcggctgatg actgccgaat
     2281 cgctcgttct ggccaatggc aagacgcgaa ttggacttga tgcccaagga aattttgccg
     2341 gtctgaatgt gagtcgcaag gctttggaaa tggcagatcc tgacgcgcag cccgacaccg
     2401 cccgcacgac agcacagagc aggcaggagc aggcccgcgc ctggcggctg gcgctgaagt
     2461 cagccaaagc tgctgacatc gcggtcggtt tcgaggatca ccgcctcacg cctccgatga
     2521 ccctcggttt gaatgtactc cgcggtgaag cgaaggattt ttccacttcg agtgcgtcgc
     2581 cgatgtccct cgatatcaag gctcgcctcg atagtggggg cgacatcgcg gccagcggaa
     2641 acgttcatgc agatggtggc acttcagacc tgaaaatcag tttggccggt gtcgaactgg
     2701 tccccgcgca agcgtatctg tcggaattcg ccaagctgaa acttgtctcc ggcaccctct
     2761 cggggtctgg gcgattgagg tacaacaacc cgacaggctc ccgattggct tatgagggca
     2821 gcgttgcgct tgaccggttg ctgctcgaag agatccaacc gaagagaaca ttcctttcct
     2881 gggattcagt tgccagcgac gatatcgttg tgacactgga gccgaatcgc ctggacatcg
     2941 gcgagttgcg ggtcgatagg ccgtccggac gtttgattat cgccgcagac cagtcggtca
     3001 atctgatgga tgtgctgcga cagccgaaca agaatgatgc gacggaagaa cagaaggcta
     3061 ttcaagtttc gggcgacaag gcgcctgccg acgagcaaca ggatgacatt caggcgcaaa
     3121 tcgtggaacg cgatctgttc ccgttcacga tcgctttcgt acgggtgtcc ggaggcacgc
     3181 tcgaatttgc ggatcagagc ctgcgtcctc agttcggcac gcgaatgcac gagttgaagg
     3241 gagttatcac cgggatcgga agcgatccga atcatggcgc caaggtgcaa cttgacgccc
     3301 gtgtcgacaa atatggatcg gcaaagatcc gcggccagat cagcatactg aagcccgaga
     3361 gatttaccga aatcgacatg actttccgta accttgaaat gacctcgctc tcaccctacg
     3421 tcgcgaaatt tgccggttac agcatcgctt ccgggaggct cgcaatggat cttcaatatc
     3481 gcgtcaagag cggtcacctg cagggtgaga acaagatcgt cctcaaacag gttgagcttg
     3541 gcgagaaagt cgaaagtccg caagccctgg atctgccgct cgaactggcc ctggctattc
     3601 tgaaggattc cagaggggta attgatgtcg aactgcccgt cagcggagac ttgaacgacc
     3661 cgcagttcga ttacggtgcg gtaatcggca aagccatcgg aaacctgctc ggcagcgtca
     3721 ttttggcacc attcaaggcg ctcggcgcac tgtttggctc cggcgacgag aagctcgaca
     3781 gtatcgattt tgaacccgga agcgctgaca tcgcccctcc cgaacgtcag aaattgggca
     3841 ctgtcgcgcg cgcgctcaag gctcgcccgt ccttgactct gatcgtaccg ccgacctacg
     3901 ctgccaatat ggacacggtt gcgctgaaat cacgtgcagt tcgcagtgaa attgtgcagc
     3961 gcatgggcat cgagttgatc cgcggtgagg acccgggccc gatcgacacg tcgaacccac
     4021 gtgttcagca cgcgatcgaa gagatgttta tccaacacta cgcaccggag gttctgtcca
     4081 cgctaaaacg caaagcgctt caaagtgaga ccatgatcgg cgatccatac gttccagccg
     4141 gcgaatccgg cccgcgcgat gcaacgcaaa ccggcaaacc catgtcagac ccattgccgg
     4201 cgttctatca aggtcttgtt gatcgcctga tcactgagga ggccgtctcc gaggagatgc
     4261 tcttgcaact ggcggctaga cgcagtgatg cgatcatcgc tgaacttgtg ttgaccggcg
     4321 gcattcccgc cgcacgaatc gttcccgggc cattggacaa gtcagcccag gcaaacacaa
     4381 tgaacgaatc gaatggagtg aatgaacgag ccgttacctt gcggctacaa cttgaggtca
     4441 gcaaatgaaa caaaatgcaa aagcgcttta tggccgattc gagaagtcgg tctggggcgt
     4501 ggcggagacg ccgcttccgc tctggaagga gcgcgcccag cgaggcttgc gtaccgtgtt
     4561 tatcttgaca cgtgacttgg tgggcggtca gctcacgcta cgcgccatga gtctggtgta
     4621 caccacgctg ctgtcgatcg taccgttgct ggcactgagt ttctcggtgc tcaaggcctt
     4681 cggcgtctat aaccagattg agcccatact gctgaagttc gtcgagccac tgggggaaaa
     4741 aggcgacgaa atcgtgcacc agatttcaat attcatcgag aagatgaatg ttggcgttct
     4801 cggcgcactc ggactgacgc tgctactgta cacagcgatc tccttgatgc agaagattga
     4861 ggaatcactc aacttcatct ggcacattcc gcatccaagg ccgcttgcag atcgtttcag
     4921 ccgctatctc agcgtgcttc tggtcggacc gattctggtg ttctcggcac tggggatcac
     4981 cgcatcggtg atgaacatcg agacggttcg cgacctgatg gaaatcaaca tactgggcca
     5041 ggccatgcag gcgatcagcc gcttgacacc gtattttctg gtgacagctg ccttcacttt
     5101 catttatctg tttatcccga atagccgcgt acgcgttgtc ccggcgttga tcggcggcgt
     5161 ggcgggtggt ttcgcctggc aaaccgcggg gtgggtgttt gccgttttca tcgcctcatc
     5221 gaacaagtat gcggcgatct attcgagtct ggcgattctg atcctgttca tgatctggtt
     5281 gtatctgagc tggctgatcc tgcttttcgg cgccagcgtc gccttctacg cccagcaccc
     5341 ggagtacctg tacgcccgta gtggtgagcc gcgcctgagc aaccgcatgc gcgagcggct
     5401 ggcgctatcg gtcatgagcc tggtttctgg ccgattcctg gccggcgaac cgatgccttc
     5461 gctggcggaa tttacccgcc tgctcggagt tccgatgcat tcgctgcaaa tagttcttgt
     5521 tgcgctggaa ggaaaccggt tcctcgcgca aagcagcgac gaaccaccgc gattcttgcc
     5581 gtcgcgcgac ccctcactga ttctggtcac tcaggttctg gaaaccgtgc gcgccgcggg
     5641 cgaggagcaa tttctctcac ccgagggcct gccggcacca ccgcccgtag atgcgttgct
     5701 caatcaaatc cagcaggccg tcgagtcgtc ggtcagcaat atgaccatgc gtgatcttgc
     5761 gctgcagatt tccgggcggg ctttgcacga accggtcttt ccaaaggaca gctaagcttt
     5821 aaccctggat ggcttcttct tcggcttgcg cccgcgcacc gcagcttcaa gcgcaagacg
     5881 accccaaggc agcatttcgg cggaagattc catnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnccgcagc ttcaagcgca agacgacccc aaggcagcat ttcggcggaa
     6061 gattccatag cttcttcggg caccgtccag tagttcatgg tttcagtgcg tccatccttg
     6121 taggtgaaat ggaaggcctc gccacccgct tgacggaagg cttcggtgct ttctgcgtca
     6181 gccttcaggt aaagcgtttc ataagcaaag atagcgaaga aaagctcgtc gcagtagaac
     6241 ccgtagccgc cgaacatcgc cttgacgcgt attgcaccga gtggcgcaaa gagttcgcag
     6301 gcgtgttcga cgagttcggg taacgggcga gccacgacgg tattacccaa gcttcaggcg
     6361 gcggcagatt tcgagcgtca gcaccgactg gttcattgaa tagaaatgta aacccggcgc
     6421 gccaccagta agtaagcgct cgcatagctc ggtaacgacg tcaaggccga aagcacggat
     6481 cgactcggca tcatcgccaa acgcttcaaa cttgcgacgc atccagcgcg gaagttcggc
     6541 gccgcaggcg tccgagaagc gcgccagctt ggagaagttg gcgatcggca tgataccggg
     6601 gacaatcggg atgacgaccc ccagcgcacg tgcttcgtcg acgaagtgga agtaggcatc
     6661 ggcattgtag aagtactgcg tgatggcgga gttggcgccg gctccggctt tcctcgcgaa
     6721 cgcggcgaga tcgtcgcgcg gcgagcgcgc ttgcgggtgc cattcagggt aggcggccac
     6781 ttcgatgcgg aaatgatctc cggtctcgct acggatgaat tcgacgagtt cgttggcgta
     6841 gcggaattcg ccggcctcgg ccattcccga aggcagatcg ccgcgcaggg caacgatgcg
     6901 gcggatcttg gcggacttgt aagcgtgcag gatatcccgg atattctcgc gcgttgaacc
     6961 gatgcacgag agatgcggcg ccgcttcgaa accttccaca gcgatctcct tgatcaccga
     7021 aaacgttctg tcgcgggtcg aaccccctgc cccaaaggtg acagagaaga actccggttt
     7081 gaggaccgca agtttgccgc gcacgatgcg cagcttgtcg accccttcag gcgtttgcgg
     7141 cgggaagaat tcgatcgaga gctcagtacg tttcatgctg ttaacattca aatagggcgt
     7201 cagttcttgc cggaaaccag aagtcccgac agcgcccacg acaccacgct gtaaaccatc
     7261 gatccgagaa ccgccggcca gaaaccggcg acgacgaagc cgtcaatcag cgaaccggcc
     7321 agccagaaca tcagaccgtt gaggacaaag atgaacaacc cgagcgtcag caaggttacg
     7381 ggcagcgtca gcaccagcaa caccggccgc accagggcat tgaccagccc gagaatcagc
     7441 gcggcgagca gggctgaacc gaacgaggcg acgtgaatcg aattcggcag caaccaggcg
     7501 acagcgagta gcgcaaccgc gttcagtatc cagagcagaa tcaatcgcat ggcaagctgc
     7561 tcgtccttct tgcagatcag tagcggtact gctctgcctt gtacggaccc tccttgggaa
     7621 cgccgatata cgcggcctgc tggtcggtca atacgctaag ctgagcgttg agcttcttca
     7681 actgcaggcg ggcgaccttt tcgtcgaggt gcttgggcag cgtatagact ccgaccgggt
     7741 aatcggcatt gcgcgtaaat aattcgatct gggcgatcgt ctgattggcg aacgacgagc
     7801 tcatgacata ggaagggtgg cccgtgccgc agccgaggtt aaccagacgc cctttggcga
     7861 gcaggatgat gcgtttgcca tcggggaaga taatgtggtc gacctgtggc ttgatttctt
     7921 cccacgtgta cttctcgacc gaagcgacat cgatttcgtt gtcgaagtga ccgatattgc
     7981 agacgatggc ctggtccttc atggccgcca tgtgctcatg ggtgatgacg tgatagttgc
     8041 cggtgcaggt cacgaaaatg tctgccttgt cggcagcgta ttccatcgtt accacgcgat
     8101 aaccttccat cgccgcctgc aacgcacaga tcggatcgat ttcagtcacc caaacctgcg
     8161 ccgacagcgc acgcatggct tgcgccgagc ccttgccgac gtcgccgtaa ccggcgatca
     8221 gcgctacctt gccggcgatc atcacgtcgg tcgcgcgctt gatgccatcg accaacgatt
     8281 cccggcagcc atacaggttg tcgaacttgg acttggtaac cgaatcgttc acgttgatcg
     8341 ccgggaactt gaggtcaccg cgttcgtgca tctggtacag gcgatgaacg ccggtggtgg
     8401 tttcttcggt cacgcccttg accgcagcca gacgcaccga gtaccaggtc gggtcggtgg
     8461 acagttttgc tttgatcgcg gcgaagagga tggtctcttc ttccgaggtc ggcttggcga
     8521 gaaccgagat gtctttctcg gcacgcgctc cgaggtgcag cagcagggtt gcgtcgccgc
     8581 cgtcatcgag gatcatgttc gaatacgcag catcgcccca ctcgaaaatc cggtgcgtgt
     8641 agtcccagta gtcggacagt gactcgccct tgacggcgaa caccggaacg ccaacggctg
     8701 cgatggcggc cgcagcatgg tcctgcgtcg agaagatgtt gcacgaggcc cagcgcacat
     8761 tggcgccaag cgccgtcagc gtctcgatca gcacggcagt ctggatcgtc atgtgcagcg
     8821 agccggtaat gcgcgcccct ttgagcggct gggcggcggc gaattcctcg cggatcgcca
     8881 tcagacccgg catttcggtt tcggcgatgc ggatttcctt gcggccccaa tcggcgagcc
//
LOCUS       DAORPM010000084         8864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1892_length_8864_cov_11.558747, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000084 DAORPM010000000
VERSION     DAORPM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1892_length_8864_cov_11.558747"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="actP"
                     /locus_tag="PLD53_05645"
     CDS             <1..639
                     /gene="actP"
                     /locus_tag="PLD53_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764712.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRC60428.1"
                     /translation="NMAAIHLANAVGGNVFLGFISAVAFATILAVVSGLTLSGASAVS
                     HDLYATVFKKGQTDSAHELRVSKITTICLGIVAVFLGIAFEKQNVAYMVMLAFAIACS
                     ANFPVLFMSVLWKDCTTKGAVAGGVVGLLSSVILTIVSASVWEAVMGNPKGSALFPYS
                     SPGLFSMSAAFLTIYLVSKMDNSKQAQLERSLYPAQKVRSETGFGAAGASGH"
     gene            complement(806..2107)
                     /locus_tag="PLD53_05650"
     CDS             complement(806..2107)
                     /locus_tag="PLD53_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60429.1"
                     /translation="MNPSSPNLYCRVVLAWVLAIAFLIPAPAMAAAENLENADSGKPF
                     ASVWRLRGDVYATGKRGSSRKLQEGSTVYVGEKVRATPNSEAVLKTGDAGIVAVRPGA
                     EFIAERFAAEGKASDHQILRLITGSLRVISGWISKLNRGEHQVVTPSATIGIRGTDHE
                     PYVLPAEMASATWQQGTYDKVNRGATELDANGGNVVIDSGKVGFARDPHSAGARTRGL
                     MTLLFPVVLAKVPEFFVPGGFDQEIDRYSEAADAISQKQLDARVGKVKPAPVQKVAPA
                     PETIAVPETRISEAQVTDKGCSPLAIGRAWLNRFDRAVEGRDIKTILDLFAPEIVVKA
                     IVRSGGGTSTLEFGRVEFVQSTLDAVTGLKDYQQRRVSLEAALVEGESVTSCKRVVVT
                     SMAIEQGLMNGRPYHFEALEEYRLEQRKGEWVAVKAQTTQR"
     gene            2319..3122
                     /locus_tag="PLD53_05655"
     CDS             2319..3122
                     /locus_tag="PLD53_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HRC60430.1"
                     /translation="MAETEIQPDSDSSPPQFELDYSDEYSRFLLHSQSEILAILRVLI
                     SKGAMITVHFDEGRSFLLSSMIALIADNREFILDIGGDKEMNQRALLAKRLIFTTKID
                     KVKVQFSLNKLSPTQSEGRAAFRGSVPETLLRLQRREFFRLSTPIANPVKLKAMVRRN
                     DNSAHHVELPLVDVSGGGVGLMVTPEQAQFFQKGDTLRDCKVMLPNEGLLVATLSVRT
                     LFSVTTRNGSRLVRLGCEFVDLPALRLTMLQRYITRVERERKAQLNGML"
     gene            complement(3181..3504)
                     /gene="fliE"
                     /locus_tag="PLD53_05660"
     CDS             complement(3181..3504)
                     /gene="fliE"
                     /locus_tag="PLD53_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRC60431.1"
                     /translation="MDTQGIDQMLSVLRATAAQAGGRTTEAQSPPAGGADFAQILQNS
                     IAQVSQTQQQAEGMAANFAAGDGNANLHDVMISLQKANISFQEMVQVRNKLVSAYHDV
                     MNMQV"
     gene            complement(3582..4955)
                     /locus_tag="PLD53_05665"
     CDS             complement(3582..4955)
                     /locus_tag="PLD53_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRC60432.1"
                     /translation="MSSGLPILIVEDDPNLREAVCDTLELAGQAVVSAQGGEEALKLL
                     DAQAVALVVSDVRMMPMDGISLLKEIRSRFPHLPVVLMTAFADVDRAVEAMRSGACDF
                     LLKPFEPRSLLEHVARYRLPETMDDDRVIATDQVTRNLFALAMRVAQTDTTVLLTGES
                     GVGKEVVAKYIHNHSARCSGPFVAINCAAIPDNLLEATLFGYEKGAFTGAQQSQAGKF
                     EQAQGGTLLLDEVTEMPLGLQAKLLRVLQEREVERVGGKKPVALDIRIIATSNRDIAD
                     AVAKGVFREDVYYRLNVFPLLIPALCQRREDIVPLARRFLAEHGGRSGRTGLRLAPSA
                     EEALYSHSWPGNVRELENVMQRAVILAAGEVVGVDELHLLASASLTRAARQGAMVEAA
                     IPASPAALSDGQRTDNMRDLEREHILETLAAVGGSRKLTGERLGMSERTLRYKLKQYR
                     EAGLFDE"
     gene            complement(5072..6274)
                     /locus_tag="PLD53_05670"
     CDS             complement(5072..6274)
                     /locus_tag="PLD53_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC60433.1"
                     /translation="MNEVAQIPAPELKAQELQRAFDVFNQVSLELTQAYEGLQGRVES
                     LTAELAVANGELRRQYQEKEALSERLSLLLNALPAGVVVLDSVALVSEANPVARQMLG
                     HAIVGSDWPTLARTTLVATDAPDEWLLGARRVAIAESPLDSAGGRLLLIHDITAAHEL
                     KAKLERNQRLAAMGEMAASLAHQLRTPLATALLYSANLAQPDLADAARTRFAGKATEQ
                     LKRLERLIQDVLLFARGESIGRDVIPAASLIADAAQMMEPLCLAKAVSFCVTSEVGNS
                     IITGSRKALSGALLNLLENALQACEGRDECVAEVKLDARTSGRYLRISVRDTGGGIAP
                     EAHERVFEPFFTTRGQGTGLGLAIALGVARAHGGSIEVNSVPGQGSEFVMLLPVGKVD
                     QQNFEDSN"
     gene            6379..8094
                     /gene="fliF"
                     /locus_tag="PLD53_05675"
     CDS             6379..8094
                     /gene="fliF"
                     /locus_tag="PLD53_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRC60434.1"
                     /translation="MAATGTNTNTSAGADNLTDRLRFALTRLSNQQKILLLVSLAAIV
                     ALVVATSTWLKHADYRILFSNISERDGGAIIAALDQMNVPYKFSDSGGAVLIPGGKVH
                     EVRLRLASQGLPKSGGVGFELMENQKFGISQFAEQVNYQRGLEGELSRTIQSIGAVQS
                     ARVHLAIPKPSVFVREELKPSASVLLNLYPGRMLEPSQIAGIQNLVAASVPNLAFSAV
                     TLIDQSGAMLSQLKSKLMEVGLDPTQIRYVREIEANAIKRVDDILSPIVGQGNARVQI
                     AADVDFSQSEQTAETHRPNTTPPDVSIRSQQTSESASATPSAQGIPGALSNQPPVPAT
                     APLTQPAVPGAGPTPPAGKPLPGQLDAAGVQAPIASVAQPISTRKDSTINYEVDKTIR
                     HTKQSVGTIKRLSAAVVINHRKDAKGVSKPLADPEMKQINELVKEAMGFNKERGDTVS
                     VANAPFTAIDRDDSGIPLWKDPDIISLVKDVFKYGAIAAIVAFLLFKVVIPLGKNMLE
                     TPPRRASKPLGEQINIVAGDDDDQTPSAAETLERKLAQARDLAQQDPKVVANIIKDWT
                     SSNAS"
     gene            8084..>8864
                     /gene="fliG"
                     /locus_tag="PLD53_05680"
     CDS             8084..>8864
                     /gene="fliG"
                     /locus_tag="PLD53_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRC60435.1"
                     /translation="MPAEDGIERSAILLIALGEDFASEVLKHLGPKEVQKLGHAMASL
                     KSVPRTQVEDVLADFRKTARDSAAVHVDTDAYVRSVLTKALGDDKASNLISRILQGGD
                     TNGIEGLKWMDAPTVADLIRNEHPQIIATILVHLENDHASDILNHFTERLRNDVILRI
                     ATLEGIQPEALKELNDVMLRLLSGSANIKKSAMGGVRAVAEILNFMGTANETTVVDSI
                     REYDPDLAQKILDEMFVFENLLDLDDRGIQLLLREIQSDSLI"
BASE COUNT         1926 a         2720 c         2391 g         1827 t
ORIGIN      
        1 aacatggctg ccatccatct ggccaacgcc gtcggcggca acgtctttct cggcttcatc
       61 tccgccgtgg cattcgccac catcctggcg gtagtgtcgg gtctgacgct gtcaggtgcc
      121 tccgccgtgt cgcacgacct ctacgcgacg gtgttcaaga aagggcaaac ggactcggca
      181 cacgaattgc gagtgtccaa gatcaccacc atctgcctgg gcatcgtcgc cgtttttctc
      241 ggcatcgcct ttgagaaaca gaacgttgct tacatggtaa tgctggcctt cgccatcgcc
      301 tgttcggcca actttccggt gctcttcatg tccgtgctgt ggaaggattg tacgaccaag
      361 ggcgcggtcg ccggaggggt cgttggcctg ctcagttcgg tgatcctgac catcgtctcc
      421 gcttcggtgt gggaagcggt gatgggcaat ccgaagggtt ccgcgctgtt cccgtattcc
      481 tcgccgggcc tgttctcgat gagtgctgca tttctgacca tctacctggt ttcgaagatg
      541 gacaacagca agcaggcgca gcttgaacgc tcgctctacc cggcgcaaaa ggtccgctcg
      601 gaaaccgggt tcggtgcggc aggggcatcc ggtcactgag tcgcttcaac aagaaagttc
      661 gtaacggaaa aacggcccga gtctctcggg ccgtttttct ttccaggcca agtatcgcgt
      721 atgcccggaa gctcacgccg accgtttgcg cagtggcaga cgggcggaca gtgagccctc
      781 gttcacgaca acaccgaagt caacgtcaac gctgcgtcgt ctgcgccttc accgccaccc
      841 actcgccttt gcgctgttcg agccgatatt cctcgagcgc ctcgaaatga tagggccgac
      901 cattcatcaa accctgttcg atagccatcg aagtgacgac cacccgcttg cagcttgtca
      961 cggattcgcc ctcgaccaaa gcagcctcaa gcgaaacccg gcgctgctgg taatccttga
     1021 gtccggtaac ggcatccagc gtgctttgaa caaactccac acgaccgaat tcgagggtgg
     1081 aggtaccgcc gccactgcgc acaatggcct taactacgat ttccggtgca aacaggtcga
     1141 gaattgtctt aatgtcgcga ccttcaacgg cgcggtcaaa gcggttgagc cacgcacggc
     1201 cgatggcgag cggcgaacag cccttgtcag tcacttgcgc ttcgctaatc cgcgtttcag
     1261 gaactgcaat cgtttccggc gcgggcgcga ccttctgaac aggtgcgggc ttcaccttgc
     1321 cgacgcgtgc gtccaactgc ttctggctga tcgcatcggc ggcttccgag taacggtcga
     1381 tttcctggtc gaatccaccc ggaacaaaga actcgggaac cttggccaga accacgggaa
     1441 acaacagggt catcagcccc ctggtccgcg cgcctgcaga atgcggatcc cgggcgaaac
     1501 ctaccttgcc gctgtcgatg accacgttgc caccgttggc gtcgagctca gtggcaccac
     1561 gattgacttt gtcataggta ccctgctgcc aagtggcgct ggccatttcg gctggcaaca
     1621 cgtaaggttc atggtcggtg ccgcgaatgc cgatggtggc ggaaggcgta acgacctgat
     1681 gctcgccgcg gttcagcttg ctgatccaac cgctgatgac gcgcagggag ccggtgatca
     1741 ggcgcaggat ctggtgatcc gaagctttcc cctcggcggc gaagcgctcc gcaatgaact
     1801 cggcgcccgg acgcacggcg acgatgccgg catcgccggt cttcaatacc gcttcgctgt
     1861 tgggcgtggc acgcactttt tcgccaacgt aaaccgtgct gccttcctgc aatttacgcg
     1921 atgacccgcg tttgcccgtg gcatagacat ccccgcgtag acgccagaca ctggcgaagg
     1981 gcttgccgct atcagcgttc tccaagttct cagcagccgc catggccggt gcaggaatga
     2041 ggaatgcaat agccagcacc caggcaagga cgacccgaca atagagattt ggtgaagagg
     2101 gattcattgt gaaaggactc aagtcatgaa taggacaagt caaagtttag tcccaaatgc
     2161 cctttaatgc tcaagtacat aggaggtcgt caaatagagc ctaatgcata cggtccagtc
     2221 gaagtgagtt gcgacagtct tggggaggca gccctctctt ttctcagcag cgggatttgc
     2281 tttaaactca ttctcgcaag ccaatatcag gatggaaaat ggcagagacc gaaattcagc
     2341 cagactcaga ttcttcgcca ccgcaatttg aactcgatta ctccgacgaa tacagcagat
     2401 tcttgttgca ctcgcagtcg gaaattcttg ccattcttcg cgtcctcatc agtaaaggcg
     2461 caatgatcac cgtccatttc gacgagggtc gttctttcct gctctcttcg atgatcgccc
     2521 tgattgctga caatcgggag ttcatcctcg acattggcgg cgataaggaa atgaaccaaa
     2581 gggcgttgct ggctaaaagg ctgatattca cgacgaaaat cgacaaagtg aaggttcagt
     2641 tcagcctgaa caaactgtcg ccaacgcaaa gcgaaggacg cgcagctttc cgcggctcgg
     2701 tgccggaaac cctgctccgc ctgcagcgcc gcgagttttt ccggctgtcg acgcctatcg
     2761 ccaatccggt caagctcaag gctatggtca gacgcaacga caacagtgca catcatgtcg
     2821 aattgccgct ggtggatgtc agtggcggtg gtgtgggtct gatggtaacg ccggaacagg
     2881 cccaattctt tcagaagggg gacaccctgc gcgactgcaa agtgatgttg ccaaacgagg
     2941 gcttgctggt ggctacgctg agcgtacgca cgctgttttc tgttacgacg cgaaatgggt
     3001 cgcgtctggt tcggctgggt tgcgaattcg tagacttgcc tgcgcttcga ctgaccatgc
     3061 ttcagcgtta catcacgcgc gtcgagcgtg aacgcaaggc acaactgaac ggcatgcttt
     3121 gaggccagag aagaaacggc acctctaagg tgccgttgtt agctgaagcg aaaacaggtg
     3181 ctatacctgc atgttcatca cgtcatgata ggccgacacc agtttgttgc gcacctgcac
     3241 catttcctga aacgaaatat tggctttctg cagggagatc atcacatcgt gcagattggc
     3301 gttgccgtct ccagcggcaa aatttgccgc cataccttcc gcctgctgct gcgtctgact
     3361 gacctgtgcg atcgagttct gaagtatttg tgcaaagtcc gccccgcccg ccggggggct
     3421 ctgcgcctcg gttgtgcggc caccggcctg tgctgccgtc gcgcgcagaa cgcttagcat
     3481 ctgatcaata ccctgggtat ccatctgtac ctccattcac ttgacacacg agcatccgca
     3541 agaaatgcgc cagaccaccc atgccacatc cgaatcggtg cttactcgtc gaacaaacct
     3601 gcctcacggt actgtttgag cttgtaccgc aaggttcgtt ccgacattcc gagtcgctcg
     3661 cccgtgagtt ttcgcgatcc accaaccgca gccagtgtct ccagtatatg ctcacgctcc
     3721 aagtccctca tattatccgt tctttgcccg tctgacagtg ctgcaggaga cgcaggaatc
     3781 gccgcttcga ccatagcacc ctgcctagcg gcccgggtta aggacgccga ggccaacagg
     3841 tgcaactcgt caacaccgac gacttcaccc gcggcaagaa tgacggcacg ctgcatgacg
     3901 ttttccagtt cccgcacgtt tccgggccat gaatggctgt atagggcttc ctccgcagac
     3961 ggcgcgaggc gcaacccggt tcgtccggaa cgaccaccgt gttcggccag gaaccgtcgc
     4021 gccagtggaa caatgtcctc gcggcgctga cacaaggcgg ggatcaacaa ggggaacacg
     4081 ttaagccggt aatagacgtc ttcccggaaa acccctttgg ctacagcgtc ggcgatatcc
     4141 ctgttggatg tagcaataat gcgaatgtcg agagcaaccg gcttcttgcc acccacccgc
     4201 tccacttcgc gctcctggag aacccgcagc agcttggcct gcagccccag gggcatctcg
     4261 gtcacctcgt cgagcaagag cgttccacct tgtgcctgct cgaactttcc ggcctgtgac
     4321 tgttgggcgc cggtaaacgc acctttctcg tagccgaaaa gcgtcgcttc aagcagattg
     4381 tccggaatgg ccgcacagtt gatggcgacg aacggccccg aacaccgggc cgaatggttg
     4441 tggatgtatt tcgcaaccac ttccttgcca actccgcttt ccccggtcag cagcaccgtc
     4501 gtatcggtct gcgccacgcg catcgccaag gcaaagaggt tgcgggtgac ctgatcggtg
     4561 gcaatcaccc ggtcatcatc cattgtttca ggcagtcggt aacgcgccac atgctcaagc
     4621 aaggacctcg gctcgaaagg cttgagcagg aaatcacacg cgccggaacg catggcctca
     4681 accgcccggt cgacatcggc aaaggcagtc atcagcacaa cggggagatg cggaaaacgg
     4741 ctgcgaatct ccttgagcaa gcttatgcca tccatgggca tcatgcgcac atcgctgacg
     4801 accagcgcca ccgcctgcgc atcgagcaac ttgagcgcct cctcgccacc ttgagcggaa
     4861 acgacggcct gcccggcaag ctcgagcgta tcgcacaccg cttcgcgcaa attcgggtcg
     4921 tcttcgacga tcagtatcgg caatcccgag ctcatattct ccagtcaacc ttggtgttgg
     4981 tattcattgt ggcccttccg tcggttgatg ctacaggcac tcaaggcagt caatccttca
     5041 tggcgctctg cgtgcctgcg tactcgtgca cctaattact gtcttcgaaa ttctgctggt
     5101 ctaccttccc gactggtaac aacatgacga attctgatcc ctgacccggc actgaattga
     5161 cttcgatgct accgccgtga gcacgagcaa ctccaagcgc aatcgccaaa ccaagtcccg
     5221 ttccttgacc gcgcgtggta aagaagggct cgaagaccct ttcatgcgct tcaggcgcga
     5281 tgccgccgcc ggtatcgcgc acactgattc tcaagtacct tccactggta cgcgcatcca
     5341 gcttcacctc ggcgacgcat tcatcacgcc cctcacatgc ctgcaaggcg ttctccagca
     5401 ggttcagaag tgcgccactc agcgctttcc gactccccgt gattatagag ttgccaactt
     5461 cggatgtgac acagaaactg accgctttcg ccaagcacaa tggctccatc atctgtgcgg
     5521 catcggctat caaagacgcg gcaggtatga catcacgtcc gatactctcg ccccgtgcga
     5581 agagcagaac gtcctgaatc aaccgctcaa ggcgtttcag ttgctcggtg gccttgccgg
     5641 caaagcgtgt tcgtgctgca tcagcaaggt caggttgtgc cagattggcg ctatagagca
     5701 atgcggtcgc cagcggcgtt cgcaactggt gcgccagcga tgcggccatc tcgcccatcg
     5761 ccgccagcct ttgattgcgc tcaagcttgg ccttgagttc atgggcagcc gtgatgtcat
     5821 gtatcagcaa caggcgtcca ccggctgagt caagcggact ctcagcgatt gcaacacgcc
     5881 gcgctccaag tagccactcg tccggagcgt cggtcgcaac cagcgttgtc cttgccagcg
     5941 tcggccaatc gctcccaacg atggcgtgtc ccagcatctg ccgggcaacg ggattggctt
     6001 cactgacaag cgcgacgctg tcaagaacga ccacgccggc gggcaaggca ttgagcaaca
     6061 gggaaaggcg ctctgaaagt gcttctttct cctggtattg tcggcggagt tcgccgttgg
     6121 ccaccgccaa ctcggcggtc aacgattcaa cgcggccctg caacccctca tacgcctgag
     6181 ttagctcgag cgaaacctga ttgaaaacgt cgaacgcgcg ctgtaattcc tgggccttca
     6241 gttccggagc cggtatttgc gccacttcat tcattcgatg gaaaatcccg tgcagttaac
     6301 agctacaata gcagaagaaa tctcaatgct tttcgctttt tgcttgatta atcgcagagt
     6361 taattcatta tcgcaagcat ggcggcaacc ggaaccaaca ccaatacttc agcgggcgca
     6421 gacaatctaa ctgatcgcct gcgttttgca ctgacgcgcc tgagcaacca gcaaaagatt
     6481 ctgctactgg tttcgctcgc cgccatcgtc gccttggtcg ttgccaccag cacctggctc
     6541 aagcatgcgg attaccgaat ccttttctcc aacatctctg aacgtgacgg cggggcaatc
     6601 atcgccgccc ttgatcagat gaacgtcccg tacaaattca gtgatagtgg cggtgccgtt
     6661 ctgatccccg gcggaaaagt gcatgaagta cgtctgcgcc ttgcctcgca aggcctgccg
     6721 aaaagtggcg gtgtcggttt cgaactcatg gaaaaccaga agttcggcat cagccagttt
     6781 gccgaacagg tcaattatca gcgcggcctt gaaggcgagc tatcgcgcac gatccaatcg
     6841 attggcgccg tccagtcggc ccgcgtgcac ttggcgatac ccaagccgag cgtttttgta
     6901 cgtgaagaac tgaaaccctc ggcgtcagtc ttgctcaacc tgtaccccgg gcgcatgctg
     6961 gaaccctccc agatcgccgg cattcagaat ctggtcgctg ccagcgtacc aaatcttgct
     7021 ttctctgcag tcacgctgat tgaccagagc ggagcgatgc tctctcagtt gaagagcaaa
     7081 ctgatggaag ttggactcga tccgactcaa atcaggtacg ttcgggaaat cgaggcgaac
     7141 gccatcaagc gcgtcgacga catcctttcg cctatcgtcg gacagggaaa tgcacgtgtt
     7201 cagatcgccg cggatgtcga tttctctcag agcgagcaaa cggccgaaac ccaccgcccg
     7261 aacaccactc cgccagacgt cagcattcga agccagcaaa ccagcgaatc ggcgagtgcc
     7321 acaccgtcgg cgcaaggcat accgggggcc ttgagcaacc agcctcctgt tcccgcaact
     7381 gccccgctga cgcaaccggc cgtacccggc gcaggaccaa ctccccctgc cggcaaaccc
     7441 ttaccgggtc agcttgacgc cgccggagtt caggcgccga tcgccagcgt tgcccaaccg
     7501 atcagcaccc ggaaggactc aacgatcaac tacgaggtcg acaagacgat tcgtcacacc
     7561 aaacaatccg ttggtacgat caagcgcctg tcggctgcgg tcgtaatcaa tcaccgtaag
     7621 gatgccaaag gcgtttcgaa gccgcttgcc gatcccgaaa tgaaacagat caacgagctg
     7681 gtcaaggagg ccatggggtt caacaaggag cgtggcgaca ccgtctccgt ggccaatgct
     7741 ccattcacgg ccatcgaccg ggacgattct ggaatcccgc tgtggaaaga cccggacatt
     7801 atttcgctag tcaaagacgt gttcaaatat ggtgccatcg ctgccattgt cgccttcctg
     7861 ctgttcaagg tggttatccc gctgggcaag aatatgcttg aaaccccgcc cagacgggca
     7921 agcaagccct tgggcgagca gatcaatatc gtggcaggcg acgacgatga ccagacgccc
     7981 tctgccgcag aaactctgga acgcaaactc gcgcaggcac gcgacctcgc ccagcaggat
     8041 cccaaggtcg tagcgaacat catcaaggac tggacaagct ccaatgccag ctgaagacgg
     8101 catagaaaga agcgcaatcc tgctgattgc gcttggtgag gactttgcct ccgaagtact
     8161 caagcacctc ggtccgaaag aagtgcagaa actggggcac gccatggctt ccctcaaatc
     8221 ggtgccccgc acacaagtgg aagatgtctt ggccgatttc cgcaagacgg cccgcgactc
     8281 tgccgcagtc cacgtggata ccgacgccta cgtccgctcg gtgctgacca aggccctggg
     8341 cgatgacaag gcatccaacc tgatttcgcg gatactccag ggcggcgata ccaatggcat
     8401 cgaaggcctc aagtggatgg atgctcccac cgtcgccgat ctcatacgca acgagcaccc
     8461 gcagatcatc gccaccattc tcgttcatct tgagaacgac cacgcaagcg acatactgaa
     8521 ccacttcacc gaacgtctgc gcaacgatgt gatcttgcgc atcgccacac ttgaaggcat
     8581 tcagccggag gcactgaagg agctcaatga cgtcatgctt cgccttctct ccggctctgc
     8641 caacatcaag aagtcggcca tgggaggcgt gcgtgcggtc gctgaaatac tcaatttcat
     8701 gggcactgcc aatgaaacca cggttgtgga ttcgatccgt gaatacgacc ccgacctcgc
     8761 acaaaagata ctcgatgaaa tgtttgtttt cgagaatctg ctggacctgg acgaccgcgg
     8821 catccagttg ctcctgcgcg aaattcagtc cgactcgctg attc
//
LOCUS       DAORPM010000085         8837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1902_length_8837_cov_11.939535, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000085 DAORPM010000000
VERSION     DAORPM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1902_length_8837_cov_11.939535"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..1304
                     /locus_tag="PLD53_05685"
     CDS             225..1304
                     /locus_tag="PLD53_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60436.1"
                     /translation="MAPHLLVDISSHGLGHLAQVAPILNQLTCLLPELRLTVRSGLSH
                     ERLRARVNGDFAYIQESSDFGFVMLDAVRIDRASTAAAYRKQHTNWRQRVADEAERLR
                     RLRPDLVLTDVAYLPLAGAARAGIPSLAMCSLNWAELFIYFFGSERWAGKIHDEMLSA
                     YCCAKSFLRLTPAMPMVDLPHIRDVGPVAALGRDCRLSLREQLACKPGEKLVLIAFGG
                     FEIQLPVELWPSIEGVRWLVPESWGLDHKDKVAFEPLGLHFTDLLCSVDAVFTKPGYG
                     IFAEAACNGTPVLYLRRDDWPEQDCLIDWLKRNARCCEIEESGLVGERLQVVLTELWE
                     QFEPSKPLPTGANEAALIIAGLLDS"
     gene            complement(1306..2322)
                     /locus_tag="PLD53_05690"
     CDS             complement(1306..2322)
                     /locus_tag="PLD53_05690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRC60437.1"
                     /translation="MLTTDFTVEFAGKSHESQQRVVSQLNPGLFGSQNVLKTIFGKTF
                     GSSVAGNPQSGVAPLMKIDLERIKTLIEFFQIGKKLRYYPEFKQDIVFDTLVVAYCVN
                     GDFIYSMEAINRDSDGYPTAFLSRENGERTPLARLKEFQLLVPDTSDLEMKLDYHRRA
                     LLGRGRQFSVGNHITLISNAGVRGVSTLDTEVAKQVILPEGPYAHTKMILLTPELNAL
                     SVTDQRTKSRTRTHAPVTISLPEGKFAGPCTIVDISEGEVRIRVRDRGTTMPLMHKGD
                     EAVIEIDLGESEKHYSIKGTVIRRSSETCVFHLVGQYANGKLSSFSPLDLLELKASLL
                     NYGK"
     gene            complement(2379..3569)
                     /locus_tag="PLD53_05695"
     CDS             complement(2379..3569)
                     /locus_tag="PLD53_05695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRC60438.1"
                     /translation="MNSICIEQLPASRKTLRLAVVTETYPPEINGVAITLSRFIDGLR
                     ERNHEIQLIRPRQNATEHPALVGGFNELLTGGVPIPRYPDLKMGFPAKRMLVSQWTRH
                     RPDLVHIVTEGPLGWSALLAAIKLRIPVCSDFRTNFHAYSLHYGIGWLKKPIIAYLRR
                     FHNRCLMTMVPTRQMQAELSDCGFHNLRVVARGVDARLFNPGRRSESLRRQWGVAPDT
                     PVAIHVGRLAAEKNLTALCAAFIGIRQRHPAARLVLVGDGPETGALRIRVPDAIFVGT
                     RQGEELATHYASGDFFLFPSLTETFGNVTLEAMASGLPVVAYNYAAAAQHLRHGDNGL
                     LASYGQTIDFVAKGGEIAARYAQEPERFRAMGHRARLTAESLDWPRVVQDFESLLLAV
                     ARDD"
     gene            3783..4391
                     /locus_tag="PLD53_05700"
     CDS             3783..4391
                     /locus_tag="PLD53_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60439.1"
                     /translation="MNAFFKALQVQRWDDHRYYHHSRINQSLHFVSAASFLAAYVMVF
                     TNPVAAALTGWLVSMTTRQAGHFFFEPKGYDVVNQASHEHKEEIKVGYNLRRKLVLIG
                     IWAVAPLTLLVDPALFGMFEPAANNAELIDQVSLIWLGVGIGGLIFRTVQLFFIRDIQ
                     TGLVWMTKILTDPFHDLKLYCTAPIHLLRGELIDPMGHEQSV"
     gene            complement(4312..6051)
                     /locus_tag="PLD53_05705"
     CDS             complement(4312..6051)
                     /locus_tag="PLD53_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC60440.1"
                     /translation="MDETFALYTAGLGALALTLPRLKRRLELSRAKHPSLAGHSRLAK
                     RIARLIPFYDYGEDTFFGSDNAPPGIVQRRRTGFLALATLYKQRFARSAALTGAAGEY
                     LSDLQFTSNYRIPFQFSRFARQHLQGGSFLQSSNGVHVTDLDGNEFHDLTGSYGVNVF
                     GYDFYRQCIVEGSARVWTLGPVLGAYHPIVIDNVRRLRAISGLDEVSFHMSGTEAVMQ
                     AVRLARYHTGRRNLVRFCGAYHGWWEDVQPGIGNPLPPRDTYTLREMDERSIHVLATR
                     KDIACVLVNPLQALHPNAGAPGDSSLVDSARRAHFDRSAYTAWLLQLRDVCSRRGIVL
                     IFDEVFVGFRLAPGGAQEYFGVRADMVTYGKTLGGGLPVGVVCGRRQLMKRFRDDLPA
                     DICFARGTFNSHPYVMGAMQVFLEQLDRPEMRKHYDGLDETWSRRAATLNHRLENADL
                     PVRVANMSSIWTVFYTRPSRYNWMLQYYLRAEGLALSWVGTGRLIFSLNYDDGDFAAV
                     AERFVAAAQAMERDGWWWEAPGITDKAIRRGILFEMLAHRFGFRPIRRFAHVPSGRSI
                     RHAEDGSVPCSRA"
     gene            6125..6979
                     /gene="asd"
                     /locus_tag="PLD53_05710"
     CDS             6125..6979
                     /gene="asd"
                     /locus_tag="PLD53_05710"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HRC60441.1"
                     /translation="MLLREDINFLLTNRLPRQLLTRFMGWFSQIEHPLVRDISIGIWK
                     RFAALDLSDARQSHFRSLHDCFTRQLRAGARPLDNNPDSLISPCDGIVGACGAISGTQ
                     VIQAKGFPYALPDLLHDPALVEYYRDGTYVTLRLTSSMYHRFHAPCDCQVDRVTYISG
                     DTWNVNPIALKRVEKLFCKNERVLIETTLTGSGNRITLVPVAAILVASVRLLFLDTLL
                     HLRYRGPNVIPCRAIFSRGDEMGWFQHGSTIIVFAPKGFALCAGIAAGQHVRMGEALL
                     RVPPSSPQ"
     gene            7197..7538
                     /locus_tag="PLD53_05715"
     CDS             7197..7538
                     /locus_tag="PLD53_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HRC60442.1"
                     /translation="MIRAILLQAGATVIAVLVAGLFAGFRGAVSAGLGGLACTLPNLL
                     FALRLKCVANRPASSYPANFFLGEFVKIAATTGLLVLIVKGYADLHGPAMLIGLVLAL
                     QASFLALWKKS"
     gene            7541..8410
                     /gene="atpB"
                     /locus_tag="PLD53_05720"
     CDS             7541..8410
                     /gene="atpB"
                     /locus_tag="PLD53_05720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRC60443.1"
                     /translation="MATGHEAAANAPNAGEYVVHHLTQLNTSGHAQASIVDFSIINLD
                     SVTFSIAMGVLGLLIMWRLAKSVTSGVPGRAQAALEMMVEMVDGQAKAIIHNKESRRF
                     VAPLGLTVFVWIFLMNSMDFLPVDFLPALWQNLTGNPHAYLRVVPTADLNVTLGMSIG
                     VLLICIWYNIKIKSLGGWVHELFTAPFGNHFLLYLPNFLMQMIEFLSKTVSHGMRLFG
                     NMYAGELLFMLIALMGAAYGGSEGMTGPLLWAGHVLAGTAWAIFHILIVLLQAFIFMM
                     LALVYIGQAHESH"
     gene            8470..8718
                     /gene="atpE"
                     /locus_tag="PLD53_05725"
     CDS             8470..8718
                     /gene="atpE"
                     /locus_tag="PLD53_05725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HRC60444.1"
                     /translation="MELVLGNVALACGLIIGLGAIGACIGIGLMGGKYIEAASRQPEL
                     MNELQTKMFLLAGLIDAAFLIGVGIAMMFAFASPFVIK"
     gene            8758..>8837
                     /locus_tag="PLD53_05730"
     CDS             8758..>8837
                     /locus_tag="PLD53_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRC60445.1"
                     /translation="MNLNATLFAQIVVFFILAWFTMKFVWP"
BASE COUNT         1753 a         2456 c         2575 g         2053 t
ORIGIN      
        1 cgaccgcatt gaggtcatcc acccagccgg caatcatgaa atcgagctgg cgcgcatgga
       61 aaacgctgac ggtttgccgc tcagtgtggc gcccggcaac ggtcatcgcg tctggattcc
      121 gctcgctgcg gggttgcagg gtgcgatgtt ggcgcgcttt gtctgaatga agccggctct
      181 tatgcaatct cttccggtat tgattcatag gcgctgaggt acccatggcc ccgcatcttc
      241 ttgtcgatat atcctcgcac gggctcggtc atctggcaca ggtggcgccg atcctcaatc
      301 aattgacttg tttgctgcct gaattgcggc tgaccgtacg aagtggcctg tcgcacgaaa
      361 gactgcgtgc gcgcgtgaat ggcgattttg cctatattca ggaaagcagc gacttcggct
      421 tcgtcatgct cgatgcggta cgcatcgacc gcgccagcac tgccgccgct taccgaaaac
      481 aacacaccaa ttggcgacaa cgcgtcgccg atgaggccga gcgactgcgt cgtctgcgcc
      541 ccgaccttgt gctgaccgac gtcgcttacc tgccgctggc tggcgccgcg cgcgccggta
      601 ttccttcgct ggcgatgtgc tcgcttaatt gggccgaact gttcatctac ttttttggca
      661 gcgaacgctg ggccggcaag attcatgatg aaatgctctc ggcctactgt tgcgcgaaga
      721 gctttctacg cctgacgcct gccatgccga tggtcgatct gccgcatatc cgcgacgtcg
      781 gaccggtcgc cgcactgggc cgggattgcc gtctttcatt gcgcgaacag ctggcctgca
      841 agccgggtga aaaactcgtt ctgatcgcct ttggggggtt tgaaatacaa ctgcccgttg
      901 aactctggcc gagcatcgaa ggcgtgcgct ggctggttcc cgagagttgg ggacttgatc
      961 acaaggacaa ggtggcgttt gaaccgctcg gcctccattt cactgatctg ttatgcagcg
     1021 ttgatgcggt attcaccaaa ccgggttatg gcatctttgc cgaagccgca tgcaatggaa
     1081 cgccggtgct ttacctgcgc cgtgacgact ggccggagca ggattgtctg attgactggc
     1141 tgaagcgcaa cgcccgctgc tgcgagatcg aggaatccgg gcttgtcggt gagcgtctgc
     1201 aggtggttct gaccgagctt tgggaacaat tcgaaccatc gaagcccctt cccaccggcg
     1261 cgaatgaagc ggcgctgatc atcgcaggcc tgcttgattc atagttcact tgccgtagtt
     1321 gagcaggctg gctttcaact ccagaaggtc cagcggactg aagctgctga gtttgccgtt
     1381 cgcgtattgc cccaccagat ggaagacaca ggtctcggac gaccgccgga tcaccgttcc
     1441 cttgatgctg tagtgctttt ccgactcgcc aagatcgatc tcgatgaccg cttcgtcacc
     1501 cttgtgcatc aggggcatcg ttgtgccgcg gtcccgtacg cgaatacgca cttcgccttc
     1561 ggaaatatca acaatcgtgc acgggccggc gaacttgcct tccggcagcg atatcgtgac
     1621 cggcgcatgg gttcgggtgc gcgacttcgt gcgttgatcg gtgaccgaca gtgcattcag
     1681 ctccggcgtc agcaagatca ttttcgtgtg tgcgtatggg ccctcgggca ggataacttg
     1741 tttcgccact tcggtatcca gtgtcgatac tcccctcaca ccggcgttgg agatcagggt
     1801 aatgtggttg cctacgctga attgtcgtcc gcggccaagg agggcgcgac gatgatagtc
     1861 aagcttcatt tcaagatcgg aggtgtccgg tacgagcaac tgaaattcct tcagtctcgc
     1921 aagcggcgtt cgttcgccat tttcacgtga aagaaaagca gtcggataac catcagaatc
     1981 gcgattgatc gcctccatgg aatagatgaa atctccattg acgcaatagg cgacgacgag
     2041 cgtatcgaaa acgatatcct gcttgaattc ggggtaatag cgcagtttct tcccgatctg
     2101 gaaaaactcg atcagcgtct tgattcgctc aaggtcgatc ttcatcaagg gggcaacgcc
     2161 ggattgcggg ttgccggcaa ccgaggaacc gaatgttttt ccaaagatcg ttttcagcac
     2221 gttctgactt ccgaaaaggc cggggttgag ctggctcaca acccgttgct gcgattcgtg
     2281 cgacttcccg gcgaactcta ccgtaaaatc agtcgttagc atatccggag catggggaat
     2341 gcccggccat tcagcgcatc gggcgggttg tgccgaggtc agtcgtcgcg ggcgacggcg
     2401 agcagcagtg attcgaagtc ctgaaccacg cgtggccagt ccagggattc agccgtcagc
     2461 cgggcgcgat ggcccatggc gcggaacctc tccggctctt gcgcgtatcg ggccgcgatt
     2521 tcaccgccct tggcaacgaa gtcgattgtt tgcccgtagc ttgccagaag gccattgtca
     2581 ccatgccgaa ggtgctgtgc cgcggcggcg tagttgtagg ccaccaccgg caagccgctg
     2641 gccatggctt ccagggttac attgccaaag gtttcggtaa ggctcgggaa aaggaagaag
     2701 tcgccggaag cgtagtgtgt cgccagttct tcgccttggc gagtgccgac gaagatggca
     2761 tcgggaaccc gaatgcgtag cgcccctgtt tcggggccgt caccaacgag gaccaaccgg
     2821 gctgcagggt gacgttggcg gatgccgatg aatgcagcac aaagcgccgt gaggtttttt
     2881 tctgcggcca gtcgtccgac atggatcgcg acgggtgtat ccggcgctac tccccattgt
     2941 cgacgcaatg attcgctgcg ccggcccgga ttgaacagcc gcgcgtcgac gccgcgagcg
     3001 accacacgca gattgtggaa accgcagtca ctcagttctg cctgcatctg tcgggtgggc
     3061 accatggtca tcaggcagcg gttatggaac cggcgcaggt aggcgatgat cggcttcttc
     3121 agccagccga tgccgtaatg caggctgtag gcatggaaat tggttcggaa atcggaacag
     3181 acaggaatcc tgagcttgat tgccgccagc agggctgacc agccaagcgg tccttcggta
     3241 acgatatgaa cgaggtccgg ccgatgtcgc gtccactggc taacgagcat gcgtttggcc
     3301 gggaagccca ttttcagatc cgggtagcgc gggatcggca cgccgccagt cagcagctcg
     3361 ttgaacccgc caaccagtgc gggatgttcg gtagcgttct ggcggggcct gatcaactgg
     3421 atttcatgat ttcgttcgcg cagaccatca atgaagcgac tcagggtgat cgccacgcca
     3481 ttgatttctg gtgggtaagt ctccgttacg acggcaaggc gcaaggtctt tcggcttgcc
     3541 ggcaactgtt cgatgcatat ggaattcacg ggctgctcgc acaggaatat gaagggtctc
     3601 aaggaaacgc ttcccaatgc tcgccagtcc cggtaacagc aatgcgacag tccgattaca
     3661 ttttcgtgta agcggaccgc tttcgccaat ttttcttcat tctttaacga acccgtagca
     3721 attcatcaat accgtcggaa cctgattcga gtgtgccaat cttcaaccat cagggagcga
     3781 ccatgaatgc attcttcaaa gccctgcaag tgcagcgctg ggacgaccac cgttactacc
     3841 accacagccg catcaaccaa tccctgcact tcgtcagtgc agcgagtttt cttgctgcct
     3901 atgtcatggt atttaccaat ccagtggcgg cagcgttaac cggctggctt gtttcgatga
     3961 ccacgcgcca ggccggccat ttcttcttcg aaccgaaggg atatgacgtc gtcaaccagg
     4021 ccagtcacga acacaaggaa gaaatcaagg ttggttacaa cctgcgacgc aagctggtgc
     4081 ttatcggcat ctgggcagta gcgccattga cgctgcttgt cgatcctgca ctgttcggca
     4141 tgttcgagcc cgccgccaac aacgccgagc tgatcgatca ggtttcattg atctggctcg
     4201 gcgtcggcat cggcggtttg atcttccgca ccgtgcaact gttcttcatc cgcgacattc
     4261 agaccgggct ggtatggatg accaagatac tcaccgaccc gtttcatgac ctcaagctct
     4321 actgcacggc accgatccat cttctgcgtg gcgaattgat cgacccgatg ggacatgagc
     4381 aaagcgtctg attggacgga aaccaaagcg atgagccagc atctcgaaca ggatgccacg
     4441 ccggatcgcc ttgtcggtga tgccgggcgc ctcccaccac cagccatcgc gttccatggc
     4501 ctgcgcggca gcgacaaaac gttcggcgac cgcggcaaaa tcgccatcgt catagttcag
     4561 gctgaagatc agccgccccg ttcccaccca ggaaagtgcc aggccttcgg cgcgaaggta
     4621 atactgcagc atccagttgt agcgcgatgg ccgcgtgtag aacacggtcc agatgctcga
     4681 catattggct acccgaaccg gcaaatcggc attctctaga cgatggttaa gtgtcgcggc
     4741 tcgtctgctc caggtctcgt cgagaccgtc gtagtgtttc cgcatttccg gtcgatccag
     4801 ttgttccaga aagacctgca tggcgcccat cacgtaggga tgggaattga aggtgccgcg
     4861 cgcgaaacag atatcggccg gcaaatcatc gcgaaagcgc ttcatgagct ggcgtcgtcc
     4921 gcagacgaca ccgaccggca atccgccgcc cagcgtcttg ccgtaagtga ccatgtcggc
     4981 acgcacgcca aagtattcct gcgctccacc cggcgccagg cgaaaaccaa cgaaaacctc
     5041 gtcgaaaata agcacgatac ctcgtcggct gcatacgtcg cgcaactgca gcagccatgc
     5101 ggtgtatgcc gatcggtcga agtgggcgcg ccgggcgcta tcgaccagag aggaatcgcc
     5161 gggtgcgcca gcgttggggt gcagggcctg cagcggattg acgagcacac aggcaatatc
     5221 cttgcgtgtc gccagaacgt ggatagaacg ctcgtccatc tccctgagtg tgtaggtgtc
     5281 gcggggaggc agcgggttgc cgataccggg ctgtacatcc tcccaccagc cgtgataggc
     5341 accacagaaa cggacgaggt ttcgacgccc ggtatggtaa cgggccagcc gcaccgcctg
     5401 catcaccgcc tcggtgccgg acatatggaa cgaaacttca tcaagcccgg aaatggcccg
     5461 cagccgtctg acgttgtcga tgacaatggg gtggtaggcg ccaagtacgg gccccagtgt
     5521 ccagactcgg gcgctgcctt cgacaatgca ctggcggtag aaatcataac cgaacacgtt
     5581 gacgccgtag gagccggtga ggtcgtggaa ttcgttgccg tcgaggtcgg ttacatgaac
     5641 gccattgctg gactgcagga agctgccgcc ctgcaaatgc tgtcgcgcga atcgactgaa
     5701 ctggaaggga atccggtagt tgctggtgaa ttgcaggtcg gagaggtatt caccggctgc
     5761 gccggtgagc gccgccgatc gagcgaagcg ctgtttgtac aaggttgcca atgccaggaa
     5821 tccggtccgg cgacgctgga cgataccagg gggtgcattg tcgctgccga aaaacgtgtc
     5881 ctcgccgtag tcgtagaacg ggatcaggcg tgcgatacgt ttcgccaggc gcgaatgccc
     5941 ggccagcgac ggatgcttgg cgcgggacag ctcaaggcga cgcttgaggc gaggcaacgt
     6001 cagggccagc gctccaaggc cggcggtata cagtgcgaag gtttcgtcca ttgatttctc
     6061 cttgccctgt cttctaccga attctcttta caaagctgtg actatcccac gtcggccatc
     6121 actcttgctg ctccgggaag atatcaactt tctgttgacc aaccgcttgc cgcggcaact
     6181 gctcacacgg ttcatgggct ggttcagtca gatcgaacat ccgcttgttc gcgatatttc
     6241 gattggcatc tggaagcgct ttgccgccct tgatctttcc gacgcccgcc aaagtcattt
     6301 tcgtagcctg cacgattgtt ttacccgaca gttgcgggcg ggggcacgac ctctggacaa
     6361 caatccggac agtctgatca gtccctgcga cggtatcgtc ggcgcttgtg gcgccatctc
     6421 cggcacgcag gtgatccagg ccaagggttt cccctacgct ttgccagacc tgttgcatga
     6481 cccggctttg gtcgagtact accgtgacgg aacctacgtc actttgcgtc tgacatcgag
     6541 catgtaccat cgctttcatg cgccctgcga ttgtcaggtc gatcgggtca cctatatctc
     6601 tggcgacacc tggaacgtga acccgatcgc cctcaagcgg gtggagaaat tgttctgcaa
     6661 aaacgagcgg gtactgatcg aaacgacgct gaccggcagc ggaaatcgga tcacactggt
     6721 accggtggcc gccatacttg tcgccagtgt tcgcctgctg ttcctcgata cgctgttgca
     6781 cctgcgttat cgcggaccca atgtcatccc ctgccgagcg atttttagcc gaggagacga
     6841 aatgggctgg ttccagcatg ggtcgacgat cattgtcttt gcgcccaaag gctttgcctt
     6901 gtgcgccggc attgcagcgg gtcagcacgt gcgtatgggc gaagcgctac ttcgagtgcc
     6961 accatcatca cctcagtgat gcgccggtaa gctgatgatt tgtagtgctt attcaagcgt
     7021 tgcgtgagga tggcgttact cgatttcgcg ttggtggcat gtgcgtgact cccgcttgtg
     7081 cagtagctta agtgctataa ttttttgctg aaacaatatt cattgacgcc gaatttcaaa
     7141 tgtcttatag ttaactaaac gacttgcagt aaatagccgt gttattctcc tcataaatga
     7201 ttagagctat tcttctgcag gcaggggcta cagttattgc ggttttggtg gctgggttgt
     7261 tcgcaggttt tcggggggcg gtatcggcag gattgggtgg tttggcttgt accttaccta
     7321 accttttgtt tgccctgcgc ctgaagtgcg tggctaatcg ccccgcctct tcatacccgg
     7381 ccaatttctt tcttggcgag tttgtcaaga tagcggcgac aaccgggctg ctggtgttga
     7441 tagtaaaggg atacgccgat ttgcacgggc ctgccatgct gatcggactg gtgcttgcat
     7501 tgcaagcgag ttttttggct ctctggaaaa agtcctgatc atggctactg gtcacgaagc
     7561 tgcagcaaac gctccaaacg ctggtgagta tgtcgtccac cacctgaccc agttgaacac
     7621 ctcggggcat gctcaggctt cgatagttga cttcagcatc atcaatctgg atagcgtcac
     7681 cttctcgatc gccatgggtg tgctcgggtt gctcatcatg tggcggctgg ccaagagcgt
     7741 gacatccggc gtccctggac gagctcaggc ggcgctcgaa atgatggtgg aaatggtgga
     7801 tggtcaagcc aaggcaatta tccataacaa ggagtcgcgc cggtttgttg cgcctcttgg
     7861 gctgaccgtg tttgtctgga ttttcctgat gaattcgatg gacttcctgc cggttgattt
     7921 tttgcctgca ctctggcaga accttaccgg caatcctcac gcctacctgc gtgtggttcc
     7981 caccgcagat ctcaatgtga cgctcggcat gtcgatcggc gtgctgctta tctgcatctg
     8041 gtacaacatc aagatcaaga gtctgggggg ctgggttcac gagctcttta cggcgccgtt
     8101 cggtaatcat ttcttgctct atctgcccaa ttttctgatg cagatgatcg agtttctgtc
     8161 caagactgtt tcgcatggga tgcggctgtt cggcaatatg tacgccggcg aattgctttt
     8221 catgctgatt gccctgatgg gtgctgccta cggtggttcg gaaggtatga ccggaccgtt
     8281 gctgtgggcg ggtcatgtgc ttgcaggtac ggcttgggca atctttcaca tcctgattgt
     8341 cttgttgcag gccttcatct tcatgatgct ggcgctggtc tatatcggcc aggcgcacga
     8401 aagccattaa tcggtatcgt ttgttgtagt tatgattgtt aacgtgtagt tcaatttgaa
     8461 ggagtggtca tggaacttgt tcttggtaat gtagcgctgg cctgtggtct gatcattggt
     8521 cttggcgcga tcggggcttg tatcggtatc ggcctgatgg gtggcaagta cattgaagcc
     8581 gcttcccgcc agcctgagtt gatgaacgaa ctgcagacca agatgttcct gctggctggt
     8641 ctgattgacg cggctttcct gatcggcgtc ggtatcgcca tgatgtttgc tttcgccagc
     8701 ccgttcgtca tcaagtaatc ggacgtctcc catcaacctt taagagggaa gataaccgtg
     8761 aatctgaacg caactctgtt tgcccagatc gttgtgttct tcattctggc gtggttcacg
     8821 atgaagttcg tgtggcc
//
LOCUS       DAORPM010000086         8800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1920_length_8800_cov_10.034534, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000086 DAORPM010000000
VERSION     DAORPM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1920_length_8800_cov_10.034534"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="PLD53_05735"
     CDS             <1..1376
                     /locus_tag="PLD53_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRC60446.1"
                     /translation="GADLLRRAMGKKKAEDMALHRDLMREGAKNKGYDEKLAMQLFDL
                     MEKFAEYGFNKSHTAAYAVVTYHTAWLKAHHCSAFIAATLSSDMDNTDSVKSFYDDAI
                     ANKLTVLGPDINTSEYRFTPVDRTTIRYGLGAVKGTGEQAVNSILKARQCSGDFKDLF
                     DFCQRVDKRMVNRRTVEALIRAGAFDSLDDHRARLMASAGIAMEAAEQAERNAMQVSL
                     FDVFDSATHTEHGPKYREVSRWKERTRLGEEKLALGYFFSGHPFHAYQAEVSRFARKP
                     LAALKAGKEPQLLAGLVVGVRTKITSRGKMAFVQLDDGTTSLEVSVFNEIFEAERAKI
                     KDDEVLIIEGKVQRDDFAGEGKVRVIADRLLTLAEARGRFARHLRLSLNGQASGPNAR
                     AAVQRLHTLLVPFTPGNCPVRLAYRNADAVCELSLGETSRVRLEDDLLTALGDWLSSD
                     NVNVEYS"
     gene            1420..1689
                     /locus_tag="PLD53_05740"
     CDS             1420..1689
                     /locus_tag="PLD53_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60447.1"
                     /translation="MHFNALSRAAFLLASSLLLNACGHTISIENYNKLQIGQSYDEVR
                     QLIGEPARCDEVLGVRSCVWGDEQHGISVSFVAGKVLLMSARNLK"
     gene            1748..2770
                     /locus_tag="PLD53_05745"
     CDS             1748..2770
                     /locus_tag="PLD53_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRC60448.1"
                     /translation="MDPLTHALLGATAAQLALGPRLGRQAWLLGAVGGVLPDADTLIR
                     SSTDPLLAIQYHRHFTHALAFIPVGGIVAALPWLVRPRHHPNWRPIVAATTIGYATHA
                     VLDACTNYGTHLLWPFSTLRVAWHWITTIGPLLTLMLLIGLVFAVRRGSRFPAAVALA
                     LSVAYVGTAAWQRERALDLQVRIAAARGHPLDRAEMFPTVGNPLLWRSVYQSGNTLYT
                     DRLRVVGRDGARWKEGSTVELLLEKDLPLEARADERVRRDFMRFSYFSAGWTARASAD
                     PSVIGDARYSLRTDGFEPIWGVRFHPGTARPTEWVDRTIKNRVPVSELWRELKGTAAG
                     YGSLPR"
     gene            3042..4043
                     /locus_tag="PLD53_05750"
     CDS             3042..4043
                     /locus_tag="PLD53_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC60449.1"
                     /translation="MDSLPRLAFGNSSALEDSDVRAMNDRIQGHTGFDISYHAADTST
                     RFRHRSSVASINGLNIVATASSPLYVDVNHAERPALLVPLSGNSYTTVEKVRFQWNAG
                     EAAILLPATARRGHDTNRSALFITIDEQRIQTTARSMLGLDAYDNVDFLSLNQPRTLV
                     MRTKEIDFNTVFRHYGELIDQFDSRPDLLGLSGIDEAIYRIMVMMLLPKQFTVLPGTD
                     RKGPNVLSDKRVNEFCEYILANLERTLTMTELESVSGVSARSLQYAFRRRFNCTPGQW
                     IVEQKLLAVRSRLLNAKLDESVTQIAILFFANLGDFSRKYRHRFGELPSQTLARVCG"
     gene            4278..4685
                     /locus_tag="PLD53_05755"
     CDS             4278..4685
                     /locus_tag="PLD53_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HRC60450.1"
                     /translation="MAARETKSTERRKFLNTALKGIAVAPLGFILPVTDVLAAKGVPS
                     NDGISSEIAKLPENDRQAKALSYKEDATMVDTAQFNRKENQFCSNCQLFSGSPGDIWG
                     PCAIFSYRAHPSIKKPFEVSANGWCKSWGPRAA"
     gene            complement(5174..5776)
                     /locus_tag="PLD53_05760"
     CDS             complement(5174..5776)
                     /locus_tag="PLD53_05760"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRC60451.1"
                     /translation="MAVLVGKQAPDFTATAVMADNEIRNFTLSAETNGKYAVIFFYPL
                     DFTFVCPSELIAFDHRLAEFKQRGVEVIGVSIDSQFTHLAWKNTPVNNGGIGQVQYPL
                     IADIKHEICRAYDVEFGAAGVAFRGSFLIDKKGTVRHQVVNDLPLGRNVDEMLRMVDA
                     LQFTEEHGEVCPAGWNKGKAGMKASTAGVAEYLSAHAKEL"
     gene            complement(5884..6177)
                     /locus_tag="PLD53_05765"
     CDS             complement(5884..6177)
                     /locus_tag="PLD53_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRC60452.1"
                     /translation="MTFLTRHLSIHGRVQGVGFRWALSAEAKALGLRGWVRNRNDGSV
                     EALVLGPPEAVEALTAWAHHGPSTAIVARVVSRDEPTSEDHESLTGFRQVPTF"
     gene            complement(6193..6738)
                     /locus_tag="PLD53_05770"
     CDS             complement(6193..6738)
                     /locus_tag="PLD53_05770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRC60453.1"
                     /translation="MIPILRQILRTGIVSEPAPAVDDALRATTQRLSEAVLKQLGRAL
                     VIRHVDAGSCNGCELEIHALNNPYYNLEAIGIKFAASPRHADLLLVTGPVARNMEVAV
                     KRAYEATPEPKLVVAVGDCGCTGGIFGESYASCGRVANVIPVDVSIPGCPPTPLQLIQ
                     GILAAIAPDRAEASRRNIPAQ"
     gene            complement(6741..8288)
                     /locus_tag="PLD53_05775"
     CDS             complement(6741..8288)
                     /locus_tag="PLD53_05775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRC60454.1"
                     /translation="MQFLEQPIDFEPLAGHAALTFVARVNAQQLNAFCGAAHTAERRM
                     VALWGSDERDRDSQSGYALHVAFDLAEGLCCLTLPMSGEDPQFPDLSLPFPVANRMQR
                     AVHDMIGARLEAGDQRPWLRHDNWPADHYPLRRDTPDSRFETRNEEYAFVTVEGDGVH
                     EVAVGPIHAGIIEPGHFRFSVMGESVLRLEERLGWKHKGIERRFCGMGLGDGARLAGR
                     VSGDSTVAYAWAYCQAAEAIGGAMPPPRALWLRALLLERERVANHLGDLGYLGNDVAV
                     SFGLMQFMRLKEDWVRLNASLFGHRLLMDRIIPGGVSVDLDAAGAKAITEQCHALGRE
                     VQNLKQIYDEHAGLQDRFLTTGRVFPHLAKRLGMIGLAGRASAQKWDLRCNHPCEPYE
                     QLEPKMAIQRNGDVAARALVRFSEISESLHLIQKIIRDLPQGSICDTTRSEADKRGFG
                     WIEGWRGDVLVALETGKDGKIRRCHCHDPSWQNWPVLEHAAIDNIIPDFPLINKSFNL
                     SYAGQDL"
     gene            complement(8297..>8800)
                     /locus_tag="PLD53_05780"
     CDS             complement(8297..>8800)
                     /locus_tag="PLD53_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRC60455.1"
                     /translation="ITFAFGMGGPVASYAGLLHMTVHSLTKSAIFFAVGHAAQKAGTQ
                     VIEDIRGLLKLSPVIGWELMIGTLAILGMPPFGVFTSEFMILTTAIKHYPWATPILLI
                     SLGVAFAAMFGKVQGMVFGKPTAQRLPHRPALIPVFLHLGLVLMLGMWIPPYLADWYR
                     QAAKLIG"
BASE COUNT         1869 a         2504 c         2567 g         1860 t
ORIGIN      
        1 gtggcgccga tctgctgcgc cgtgcgatgg gcaagaagaa ggccgaggac atggctttgc
       61 atcgcgacct gatgcgcgag ggggcgaaga acaagggcta cgacgaaaag ctcgccatgc
      121 agcttttcga tctgatggag aagttcgccg agtatggctt caacaagtcg cacacagcgg
      181 cctatgccgt ggtcacctat cacacggctt ggctcaaggc gcatcattgt tcggccttca
      241 tcgcggcaac cttgtcatcg gacatggaca acaccgactc ggtgaagtcg ttttacgacg
      301 atgccattgc caacaaattg accgtcctcg ggccggatat caacacctcg gaataccgct
      361 tcactccggt tgatcgcaca accatccgct atggtctggg cgccgtcaag ggcacgggcg
      421 agcaggcggt caactcgatt ctcaaggcgc gtcagtgttc gggggatttc aaggatctgt
      481 ttgatttctg ccaacgcgtt gacaaacgca tggtcaatcg gcgcaccgtt gaagccctga
      541 ttcgcgctgg cgcctttgat tcgctggacg atcaccgggc gcgtctgatg gcttcagccg
      601 gaatcgccat ggaggccgcc gaacaagccg agcgcaacgc catgcaggtc agcctgtttg
      661 acgtgttcga cagtgcgact cacaccgaac acggaccaaa gtaccgtgaa gtttcgcgct
      721 ggaaagaacg gaccagactt ggcgaagaaa aactggcact cggttatttc ttctcggggc
      781 acccattcca tgcctatcaa gccgaggttt cgcgcttcgc gcgcaaaccg ctcgctgctc
      841 taaaggccgg caaagagccg caactgctgg ccggtctcgt ggtcggtgtg cgcaccaaaa
      901 ttacttcgcg cggaaagatg gcgtttgtgc aactcgatga cggcacgaca tctctggagg
      961 tttccgtatt caacgaaatc tttgaagccg aacgcgccaa gatcaaggat gacgaggtat
     1021 tgatcatcga gggcaaggtg cagcgcgatg actttgcagg cgaaggcaag gtgcgcgtaa
     1081 tcgccgatcg actgttgacg ctggccgagg cacgcggacg gtttgccagg catctgcgtc
     1141 tatcactcaa cggccaggca agcggcccaa atgcaagagc tgcggtgcag cgcctgcata
     1201 ccttgcttgt gccctttacc ccaggcaatt gccctgttcg actcgcctac cggaacgcgg
     1261 atgcagtctg tgaattgtcg ctgggtgaaa ccagccgggt acgactcgaa gacgatttgc
     1321 tgaccgcgct gggcgactgg ctgagcagcg acaatgtcaa tgtcgagtac agttgagcgc
     1381 tgttgagtta tccggtgcag gtttacggag aggactgata tgcatttcaa tgctttgagc
     1441 cgggcggctt ttctgctggc atccagcctg ctgttgaatg cctgcgggca tacgatcagc
     1501 atcgagaact acaacaagct ccagatagga cagtcctacg acgaggtcag gcagctcatc
     1561 ggcgagccgg cacgttgcga cgaagtgctc ggcgttcgca gctgcgtctg gggtgatgag
     1621 cagcacggca tcagcgtcag tttcgttgcc ggcaaggtat tgctcatgtc tgcccggaac
     1681 cttaagtgac agggcggttt caagtccgaa gtaaaacgca ttccccactg caagctggag
     1741 cttgaacgtg gaccccttga ctcacgcatt gctcggcgcg accgccgcac agcttgccct
     1801 gggcccgcgc cttggccgcc aggcgtggct gctcggcgcc gtcggtggcg tgctgcccga
     1861 cgccgacacc ctgatccgga gttcgacgga tcccttgttg gcgatccagt accatcgcca
     1921 cttcacgcac gcgctggcgt tcatcccggt gggcggtatc gttgcggcct tgccgtggtt
     1981 ggtgcggccg cggcatcacc cgaactggcg gccgattgtg gcggccacga ccatcggtta
     2041 tgcaacccat gccgtactcg atgcctgcac caactatggg acgcacctgc tgtggccctt
     2101 ttcgacgctg cgcgtcgcct ggcactggat cacgaccatc ggccccctgc tgactctgat
     2161 gctgctgatc ggattggtct tcgccgtccg tcgtggctca cgattcccgg cagctgtggc
     2221 gctggctctc agtgttgcct acgtcgggac ggctgcctgg caacgcgaac gcgcgctcga
     2281 cctgcaggtc cggatagccg ccgcccgtgg ccatccgctc gatcgcgccg agatgtttcc
     2341 gaccgtcggc aatccgctgc tctggcgatc ggtctaccaa tcgggcaaca cgctgtacac
     2401 cgaccgtttg cgcgtcgtcg gcagggatgg tgctcgctgg aaagagggca gtacggtcga
     2461 gttgctgctg gagaaggatc tgccgctgga ggccagggcc gacgagcgcg tccgccgtga
     2521 cttcatgcgc ttcagctatt tctccgccgg ttggactgcg cgtgccagcg ccgaccccag
     2581 cgtcatcggc gacgcccgct actcgctgcg taccgacggc ttcgaaccga tctggggcgt
     2641 tcgcttccac cccggcacgg cacggccaac cgagtgggtc gatcggacga tcaagaaccg
     2701 cgtgccagtc agcgagttgt ggcgcgagct aaagggtacg gcagccgggt acggttcact
     2761 ccccaggtga cttttccggt cggacaagcg gctggtaaaa acccctgcag cgttctgaga
     2821 gcaacttgtc ttatgacccc agcgtgagaa cgttaggaca ctgctgattg tgcgtactaa
     2881 tgcaccatcc taaggctcga tgcaatattt tagggcggaa aaaatcatgc aacggcattt
     2941 tcagacagct taaccaattg attatcaaca agtagttttt atggaataac atttgcttat
     3001 agatcaaaaa catttttaat atagctctga cgagatcatc tatggattct ctgccaaggc
     3061 ttgcttttgg aaattccagc gcgcttgaag atagcgacgt tcgtgcgatg aatgatcgca
     3121 tccagggaca caccggtttt gatatttcct atcacgctgc agatacttcc acaagattca
     3181 ggcaccgttc gtcagtcgcc agtattaacg gcttgaacat cgtcgccaca gcgagctcgc
     3241 cgctttacgt cgacgttaac catgccgagc gaccggcgtt gttggttccg ttaagcggca
     3301 atagctatac cactgtcgaa aaggtccgct ttcagtggaa tgcgggagag gcagccatac
     3361 tgttgcccgc tactgctcga cgtggccatg atactaatcg ttctgcgtta tttatcacga
     3421 tagacgagca gcggatacaa actacggccc gttcgatgct gggtctggac gcctacgata
     3481 atgtcgattt tttgtcgctg aatcaaccaa ggacgttggt gatgcgtacc aaggaaatcg
     3541 atttcaacac cgtattccgc cactacggag agttgatcga tcagtttgac agcagaccag
     3601 atctgcttgg gctgagcggg atcgatgagg caatttaccg aatcatggtc atgatgctct
     3661 tgccaaaaca gtttaccgtt ttgcctggca ccgatcgaaa agggccaaat gtgctgtctg
     3721 acaagcgtgt gaacgaattc tgcgaataca tcctcgccaa tctggaacgt acgctgacaa
     3781 tgactgaact ggagtctgtt tccggtgtat ccgcacgttc gttgcagtat gctttccgcc
     3841 gaaggttcaa ttgcactccg ggtcagtgga ttgtcgaaca gaaacttctt gccgttcgca
     3901 gccgactgct taatgcaaaa ctcgatgaaa gcgtcacgca gattgccata cttttcttcg
     3961 caaatcttgg agacttttcc cgtaaatatc gccaccgctt tggcgagcta ccttctcaaa
     4021 ccctggcaag ggtatgcggc taaaagcgct tcgttttccc gatagcattt cgcttttctg
     4081 gtagtaaacc tcacgcaatc cctttagagt tgtgcttgcg tcatgtcgaa tgcacggttg
     4141 cgttcctcga ggcggaactt tcaatttccc tgttatgtat gaggttgaag gtacgctaac
     4201 tgaacctgtc atcgcctcga ctgacataat cgcttcaaga atggatcatt tttcaaataa
     4261 tcaggagaaa attaatcatg gctgcgcgag agaccaaaag caccgagcga aggaagtttt
     4321 tgaatactgc cctaaaaggc attgcggttg caccgctggg atttattctg cctgtcaccg
     4381 acgtactggc ggctaaagga gtcccatcga acgacggcat ttcctccgaa atcgccaaat
     4441 tgccagaaaa tgatcgtcaa gccaaggctt taagctacaa ggaagacgca acaatggtgg
     4501 acaccgccca atttaaccgg aaggaaaatc agttctgtag caattgccaa ttatttagcg
     4561 gatcaccggg tgacatatgg ggaccatgcg cgattttctc ctaccgggca catccctcaa
     4621 tcaagaaacc attcgaggtt agcgcgaatg gctggtgtaa gtcttgggga ccgcgagcgg
     4681 cttgaatatc ttccgtttgt cctgccccag ccagcccctt ggttgccaat tcaaatggct
     4741 tgactgaagg tgccagatcc tcagatttcc cggcggcatt tcgtcgtgcc cgttccgttc
     4801 acgatcaccc gcctctgatc gccagatcga tcgtgcgtca tcgcctggct cccgccggct
     4861 acatagcggg aattagagcg gcgacgccta ggctgggttc cacacgctac aacggcgtgg
     4921 cacgtattga tggattgcgc acctttctgt ttcgtgtcga cggtgctgga agacgtagcc
     4981 tgtcactccc tgaacttggc tcacttgctg tgtgccccaa gatgcgctaa cgcatcccgc
     5041 gattcggaca cggttatgta cacgggcagg atcaattgat acttgacaca gaacgcatcg
     5101 ggccaagcaa tcactgtcgc aaaaaaaaga ccgcgccttc caacgcggtc ttttttgatt
     5161 gaaccccgaa gacttaaagc tccttggcgt gagccgacaa atactcggca acgccagcgg
     5221 tagatgcctt catgccggct ttgcccttgt tccagccagc cgggcagact tcgccatgct
     5281 cttcggtaaa ttgcagtgca tcgaccatgc gcagcatttc gtcgacattg cggccgagcg
     5341 gcaggtcatt caccacctgg tgacgaacgg tgcccttctt gtcgatcagg aatgaaccgc
     5401 ggaaggcgac gccggcagcg ccaaattcga cgtcgtaggc gcggcaaatc tcgtgcttga
     5461 tgtcggcgat cagcgggtat tgcacctggc caatgccgcc attgttgacc ggcgtattct
     5521 tccacgccag gtgagtgaat tgggagtcga tcgagacgcc gataacttcg acgccgcgct
     5581 gtttgaattc ggccagccgg tgatcgaagg caatcagttc ggacgggcat acaaacgtaa
     5641 aatcgagagg gtaaaagaaa atgaccgcat attttccatt ggtctcggcg gacagggtga
     5701 aattgcggat ttcattgtcg gccatgacgg cggttgctgt gaaatcaggg gcttgcttgc
     5761 caacgagtac ggccataatt cttctccttc agtgggtttt gaacaaagtg gggatcaata
     5821 acggacgagt gccgaatcca gtgccactta gtcagcatag cgtcccggca agttccccgc
     5881 aaatcaaaat gtcggtacct gcctaaatcc agtcaatgat tcgtgatctt cactggtggg
     5941 ttcatccctg ctgacaacgc gtgcgacgat cgcggtcgaa gggccgtggt gcgcccaagc
     6001 ggttagcgct tccactgcct cgggcggacc aagaacgagc gcctcaacgc tgccatcatt
     6061 gcggttgcgg acccagccgc gcagccccaa tgccttcgct tcggcactga gtgcccagcg
     6121 aaagccgacg ccctgtactc ggccgtggat ggaaagatga cgggtgagaa aggtcacgtc
     6181 tgaaacggga ttctactggg ccgggatatt cctgcgactg gcttcggccc tgtcgggggc
     6241 gatggcagcg aggatgccct gaatcagttg cagcggcgtg ggcgggcaac caggaatcga
     6301 tacgtcaacg ggaatgacat tggctacgcg gccacaggag gcatagctct ccccaaaaat
     6361 gcccccggtg cagccacagt cacccaccgc gaccaccagc ttgggttccg gcgtggcctc
     6421 gtaggcgcgc ttgacagcga cttccatgtt ccgggcaacc ggcccggtga ccagcagcag
     6481 gtcggcatga cgcgggctgg cagcgaactt gatgccgatg gcctcgaggt tgtaataggg
     6541 attgttcagg gcgtggattt cgagctcgca gccgttacat gaaccggcat cgacatgacg
     6601 tatcaccagg gcgcggccaa gttgcttgag gaccgcttcg gacagccgct gcgtggtggc
     6661 gcgcaaggca tcgtcaacgg caggcgccgg ctcgctgacg attccggtgc gaagtatttg
     6721 gcgaaggatc ggaatcatga tcagagatcc tgtccggcgt aggagagatt gaaggatttg
     6781 ttgatcaacg ggaagtcagg aatgatgttg tcgattgcag cgtgttcgag caccggccaa
     6841 ttttgccacg acgggtcgtg gcaatggcaa cggcgaatct tgccgtcctt gccggtttcc
     6901 agcgcgacga gaacgtcgcc ccgccagcct tcaatccagc cgaatccgcg cttgtcggct
     6961 tcactgcggg tcgtatcgca aatgctgcct tgaggcagat cgcgaatgat cttttgtatc
     7021 aggtgcagcg actcgctgat ttcggagaaa cggaccaacg cgcgcgcggc gacgtcgccg
     7081 ttgcgttgaa tggccatctt cggttccagc tgctcatacg gttcgcacgg atggttgcag
     7141 cgcagatccc acttttgcgc gctggcgcgg ccggcgaggc cgatcatgcc aaggcgtttg
     7201 gcaagatggg gaaagacccg gcctgtcgtg agaaagcggt cctgcaaacc cgcatgctcg
     7261 tcgtagattt gcttcaagtt ttgaacttcc ctgccgaggg cgtggcattg ctcggtgatc
     7321 gccttcgcac ccgctgcgtc aaggtcaaca gaaacgccgc ccggaatgat ccgatccatc
     7381 aacaaccggt ggccgaacag gctggcgttg agccggaccc agtcttcctt gagacgcatg
     7441 aattgcatca gcccgaacga tacagccaca tcattgccga ggtatccaag atcgcccaga
     7501 tgattcgcca cacgttcgcg ttcgagaagc aaagcacgca accagagggc gcgcggcggt
     7561 ggcattgcac caccgatggc ttcagccgcc tgacaatagg cccaggcgta ggcaaccgtc
     7621 gagtcgccgg aaacgcggcc agccagccgg gcgccgtcgc cgagccccat gccgcaaaat
     7681 cgtcgctcaa tgcccttgtg tttccagccc agtcgctcct ccaggcgaag cacgctttcg
     7741 cccatcaccg aaaagcggaa atggcccggt tcaatgattc cagcatgaat tggaccgacg
     7801 gcgacttcgt gcacgccgtc gccttcgaca gtgacgaatg cgtactcctc gttccgcgtt
     7861 tcgaatcggg agtcgggcgt gtcgcggcgt aagggataat ggtcagccgg ccagttatcg
     7921 tgacgcaacc aggggcgctg atcaccggct tcgagtctgg cgccgatcat gtcatgcacg
     7981 gcacgctgca tgcggttggc gactggaaac ggcagcgaaa ggtcggggaa ctgcggatcc
     8041 tcgccggaca tcggcagggt cagacaacac agcccctctg ccagatcgaa agcgacatgc
     8101 agggcatagc cggattgact atcgcggtcc cgttcatcgc tgccccaaag cgccaccatg
     8161 cggcgctcgg cggtgtgcgc ggcgccgcag aaggcgttga gctgctgggc gttgacccgc
     8221 gcaacgaaag tcagcgcggc gtggccggcc aggggttcga agtcgatggg ttgttcgagg
     8281 aattgcatgc gtgttctcaa ccgatcaatt tggccgcctg ccggtaccaa tcggcgaggt
     8341 agggaggtat ccacataccg agcatgagca ccagtccgag atgcagaaag acggggatca
     8401 gcgccggacg gtgcggcagg cgctgtgccg ttggttttcc gaagaccatg ccctgcacct
     8461 tgccaaacat ggcggcaaag gccaccccca gcgagatcag caggatcggc gtggcccagg
     8521 ggtagtgctt gatggctgtg gtcagaatca tgaactcact ggtgaacacg ccaaagggag
     8581 gcatgccgag gatggccaac gtgccaatca tcagttccca gccgatgact ggcgaaagct
     8641 tgagcaggcc ccggatgtct tcaattacct gtgtgccggc cttttgcgcg gcgtggccaa
     8701 cggcgaagaa tattgccgat tttgtcaaag agtgcaccgt catgtgcagc aacccggcat
     8761 agctggcgac cgggccgccc atgccgaaag caaaggtgat
//
LOCUS       DAORPM010000087         8796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1922_length_8796_cov_11.267132, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000087 DAORPM010000000
VERSION     DAORPM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_8796_cov_11.267132"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="PLD53_05785"
     CDS             <1..1580
                     /locus_tag="PLD53_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC60456.1"
                     /translation="KHGAAYVRVEQFLEWMTAGYRRVLTAALDRRWIVMLAFVFVAAT
                     SVVLLKALKSELAPVEDRGIILGVFLGPEGATLDYTDKYARQLEGIYSDTKDVERYFV
                     VAGNPTVSQGISFVGLSDWKVRKRSAGEVVKELFPKFMGIPGVLAFPVQPPSLGQSPR
                     ERPINFVVVTSATYPELLETTNAILAEVAKNPGLTNVDTDLKLNKPELSVNVNREKSS
                     DTGVQIETIGRTLETMLGGRQVTRFKQDGEQYDVIVQLADAERTRPSDIRDIFVRAKD
                     GGMIPLDSLISVTETLSPRELNHFGQRRAVTITANLAPGYTMGEALGFMEQTANRVLR
                     PGYAIDYAGQSREFKTSSSSLAITFVLALAFIYLVLAAQFESFRDPFIIMLTVPLSMT
                     GALGALWLTGGTLNVYSQIGLVTLVGLITKHGILIVEFANQLQEKGSDIKRAVIEASE
                     LRLRPILMTTGAMVLGAIPLALATGAGAESRQQIGWVIVGGLLLGTFFTLFVVPTVYS
                     LLARRKNPTLAETAQSA"
     gene            1833..2336
                     /locus_tag="PLD53_05790"
     CDS             1833..2336
                     /locus_tag="PLD53_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60457.1"
                     /translation="MSIIFVKTPKGIAEIEQRGGGLTPRVRRVLIMLDGKRTDDDIRA
                     MALVDDLEQTLETLVEAGYIESPKQPEASAATTGAGIVNTVTFREVPDKPDPKEMEMA
                     KNYIMNTLKTFCGPMAHLSIYEAVTAARTHEELRAQFVPWHNAITLTRDGRRRAEDLC
                     ADLLKVI"
     gene            complement(2355..3155)
                     /locus_tag="PLD53_05795"
     CDS             complement(2355..3155)
                     /locus_tag="PLD53_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC60458.1"
                     /translation="MHSAALKVFITGASSGIGSALATHYARKGAVLGLAARRGDQLAI
                     LADTLFSEGAKHVSCYPLDVTDADALRSAAIDFITSEGARPPDIVIANAGVSSGTLTE
                     CSEDLETFRRIFDINVYGMAATFSPFIAAMRQAAKAGIPVRLVGIASVAGVRGLPGAE
                     AYSASKSAAITYLESLRLEMRESGIKVVTIAPGYIETPMTAINPYRMPFLLTADDAAR
                     RFARAIERGTSYSVIPWQMGIVAKVLRLLPNALYDRLFVNAPRKPRVL"
     gene            complement(3188..3847)
                     /locus_tag="PLD53_05800"
     CDS             complement(3188..3847)
                     /locus_tag="PLD53_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HRC60459.1"
                     /translation="MQRMKTFITGWFAAVVLALVSMALPAHAQLAVGRDYTVIEPAQG
                     TDDPGRIEVVEFFSYACSHCNEFHPHVSKWASKLPGDVTFKRVPVSFNRPQWANLARL
                     YYALEATGDLAKLDGEVFHALHDKGLRLIDDDSIQEWAVSQGVDAKKFSDAYNSFGVV
                     SKTKRADQMAQNFRIRGVPALAVDGRYLVVGQSVKSTAELLPLTDKIINKVRAERKLK
                     K"
     gene            complement(3858..4451)
                     /locus_tag="PLD53_05805"
     CDS             complement(3858..4451)
                     /locus_tag="PLD53_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRC60460.1"
                     /translation="MKPRKPSKATARRKSGGGMLVGIFLGLVLGVLCAAGVVWYIFKT
                     PPPFSNKGQPSPSAAQQAGATVAPLALPGKPGDPVPQNGDKPRFDFYKILPGNAEAIP
                     DPKPTEVVKDKGEKENMLKESVYLQTGSFQNVDDADNQKTKLALMGVEASVQQVMLQD
                     RVWYRVRLGPFRKMDEVNNMRSDLAKQGIEASLVRKD"
     gene            complement(4485..6269)
                     /gene="argS"
                     /locus_tag="PLD53_05810"
     CDS             complement(4485..6269)
                     /gene="argS"
                     /locus_tag="PLD53_05810"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRC60461.1"
                     /translation="MTHTIKAHLAELMRAALISVVPEHADANIILERPKQSSHGDFSC
                     PLAMQLARSMKRNPRELAQLLRSELPKSPFVSSTEIAGAGFINFHLLPSAKLEVVRSI
                     LVQGESFGRSTLGGRKKVQIEFVSANPTGPLHVGHGRGAAYGASLSNLLSFAGWDVTR
                     EYYVNDAGRQMDILALSAWLRYLELYVDTLGCVAFPPNAYQGDYVREMAAQMKLAHDD
                     KYCHPAATVLRGTPGLPDLGRTDDDAKAQCESHLDALIANAKTLLGEDWNYVHNHVLT
                     EQLADGRGDLEEFGVRFDVWFSEKSLFDTGLVARCVARLEKTGHLYTQDGALWFKSTA
                     FGDEKDRVVQRDNGLYTYFASDIAYHLNKYERGFERLINIWGADHHGYIPRVRGALAA
                     LGLDPNLLDVALVQFAVLYRNGQKASMSTRSGEFVTLRTLREDVGNDACRFFYVLRKS
                     DQHLDFDLDLATSQSNENPVYYIQYAHARICSLLAQWNDNADDEGSSLGNADLARLDT
                     PHEQALAARLSEFPEVIESAAGELAPHLIAFYLKDLAGEFHSYYNAERMLVDDDALTN
                     ARIALAVAVRQVIRNGLSILGVSAPQSM"
     gene            complement(6438..7040)
                     /locus_tag="PLD53_05815"
     CDS             complement(6438..7040)
                     /locus_tag="PLD53_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRC60462.1"
                     /translation="MNVNPYHNVFEDIQALLTGTLLVAFAIVLFRQAGLLTGGTPGLT
                     FLAHYASGIPFGTLYFLINIPFYALAFKAFGTLFSLKTFCAVALISFYSEWFPHWINI
                     ESVDPVFAAILGGLLAGTGLLMLIRHQVCVGGIGILAIYLQQRHGWRAGILQMIADAI
                     IVCGALFVVDEERVVLSILSVLVLNIVLTVNHRPGRYFGQ"
     gene            7212..8627
                     /locus_tag="PLD53_05820"
     CDS             7212..8627
                     /locus_tag="PLD53_05820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC60463.1"
                     /translation="MKVKTLDGREYDLQQATLDSLRMRLKGSVLATGDAGYDESRTVW
                     NAMIDQTPALVVRCLGTADVIACVQFARDNRLLLCIKGGGHNIAGLATVDGALMLDLS
                     LMRGVWVDAQRQIAHAQAGCLLGDVDRETQLHGLATVLGFVSMTGIAGLTLGGGFGYL
                     TRRWGWTTDNVIGMDVVTADAGLVRATSDENPDLFWGLRGGGGNFGVVTGIDYALHPV
                     GPEVVGGIVAWAADNAPEVLRLYRALAEAAPPELALVTFMRPAPPAPWLPKDMHGKPI
                     VAILACHCGNPEEGEKAVAAIKSFGTPIGDVLVRRPYTQLQALLDGTQPKGRRYYWKS
                     EYLAQIDAGLCEKFIAHSAKIRSPHSAMILFQIGGALNRFEDEHSPVGNRDARYVLNI
                     SGAWEQATDDDSNIGWAREAWDDLKAFSTGGTYINFLTQDEGAERIEAALGKNMSRLA
                     EVKLRWDPENVFRTNRNIKPA"
BASE COUNT         1899 a         2461 c         2625 g         1811 t
ORIGIN      
        1 aaaagcatgg cgccgcctac gtgcgtgtcg aacaattcct cgaatggatg actgctggct
       61 accggcgggt gctcaccgct gcgcttgatc gccgctggat tgtcatgctg gcgttcgtgt
      121 tcgtcgcggc caccagcgtt gtcctgctca aggcgctcaa atcggagctg gcgccggtcg
      181 aggatcgcgg cattatcctg ggggttttcc tcgggccgga gggtgctacg cttgactaca
      241 ccgacaagta cgcgcgccag cttgagggaa tctacagcga caccaaggac gtcgaacgtt
      301 atttcgtcgt ggccggcaac ccgacggtca gccagggtat ttccttcgtt ggtctgagcg
      361 actggaaggt gcgcaaacgc agcgccggcg aggtggtcaa agagttgttt ccaaaattca
      421 tgggcatccc gggagtgctg gccttccccg tacaaccgcc ttcacttgga caaagcccgc
      481 gcgagcgtcc gatcaatttt gtcgtcgtga catcggcgac ctaccccgaa ttgctggaaa
      541 caaccaacgc tattctcgcc gaagtggcga agaatccggg gctgaccaac gtcgataccg
      601 acctcaaact caacaagcct gagctttcgg ttaacgtcaa ccgcgagaag tcttcggata
      661 ccggcgtgca gattgaaacc atcggccgga cgctggagac catgctgggt gggcggcagg
      721 tgacgcgatt caaacaggat ggcgagcaat acgacgtgat cgttcagctt gccgacgccg
      781 agcgaacccg cccttcggat attcgcgata tatttgtgcg tgcaaaggac ggcggcatga
      841 ttccgcttga cagcctgatt agcgttacgg aaacgctcag cccgcgcgaa ctcaatcatt
      901 tcggtcagcg tcgtgcggtg acgattaccg ccaatctggc gcctggctat accatgggcg
      961 aggctttggg tttcatggag caaacggcca accgagttct gaggccgggc tacgccatcg
     1021 actatgcggg ccagtcacgc gaattcaaga cttccagttc gtcgctggca atcaccttcg
     1081 tgctggcgct ggcgtttatc tatctggtgc tggccgcaca gttcgaaagc ttccgtgatc
     1141 ccttcattat catgctcacc gtgcctctgt cgatgaccgg tgctctcggc gctttgtggc
     1201 tcaccggcgg cacgcttaac gtctatagcc agattggcct ggtgacgctg gtcggtttga
     1261 tcaccaagca cggcattctg attgtcgagt ttgccaatca gttgcaggaa aagggtagcg
     1321 acatcaaacg ggcggtgatc gaggcgtccg agcttcggct gcggccgatt ttgatgacga
     1381 cgggtgccat ggtacttggc gccattcctc tggcgctggc gacaggtgcc ggtgccgaaa
     1441 gccggcagca aatcggctgg gtaattgtcg gtggcctgtt gctcggcacc ttcttcacgc
     1501 tttttgtggt gccgacggtg tatagcctgc tggcacggcg caagaatccc acgcttgcgg
     1561 aaaccgcgca gagcgcttga cacggttgcg gccggcacac gagatcgtgc gcaccgccat
     1621 gccatgtcaa ccgactggca tcgatagcga cagctcgctc atggctgcgc agaatacaat
     1681 tcacccgata caaaatcatc cgggtggatc aaataacaat cgtcaaaaac tcgcaaaaat
     1741 aggacattat cgttgttttt gcagagtcga atccgttaca gtaaacaaaa ataagtcaat
     1801 aaaaacatac tgctgtatgg aggaggtatt caatgagtat tatttttgta aagacgccga
     1861 agggcattgc ggagatcgaa cagcgtggtg gtggcctgac accgcgcgtg agacgggttc
     1921 tgatcatgct ggacggcaaa cgcacggatg acgatattcg cgccatggcg ttggtcgacg
     1981 atttggaaca gactctcgaa acgttggtgg aagccgggta cattgagtct ccaaaacagc
     2041 ccgaagcctc agccgcaacc accggggccg gtatcgtcaa tactgtcacg tttcgcgaag
     2101 taccggataa gcccgacccg aaggagatgg agatggccaa gaactacatc atgaacacgc
     2161 tgaagacgtt ctgcggcccg atggcgcatc tgtcgatcta tgaagccgta actgctgcca
     2221 gaacccatga agagcttcgc gctcagtttg tcccctggca caacgccatc actctgacgc
     2281 gcgacggacg tcgccgcgct gaggatctct gcgccgacct gcttaaagtg atttgatcgt
     2341 cagtcttgaa ccggctacag aactcttggt tttcttgggg cgttgacgaa taatcgatca
     2401 tagagcgcgt tgggcagcag gcgcagaacc ttggctacga tgcccatctg ccagggaatc
     2461 acagagtagc tggtgccgcg ttcgattgcg cgtgcgaagc ggcgcgcggc gtcatcggct
     2521 gttagcagaa atggcatccg gtagggattg attgccgtca ttggcgtttc gatgtagcca
     2581 ggggcgatgg tgacgacctt gattccggat tcgcgcattt caagccgcag gctttcgagg
     2641 taggtgatcg ctgccgattt ggaggcgctg taggcttcgg caccgggtaa gccgcgtaca
     2701 cctgccaccg atgcgatacc gaccaggcgg accggaatac ccgccttggc ggcttgccgc
     2761 atggcggcga tgaagggtga aaaggtcgct gccatgccat agacgttgat gtcgaagatg
     2821 cgccggaagg tttcaagatc ttcggagcat tccgtgaggg tgccggacga gacgccagcg
     2881 ttcgcgatca cgatgtcggg ggggcgcgcg ccttcactgg tgatgaagtc tatcgctgcc
     2941 gaacgcagtg cgtcagcatc ggtaacatca agtggatagc aggaaacgtg tttcgcgcct
     3001 tcgctgaaca gcgtatcggc gagtatcgcg agttgatcgc cacgccgggc agcaagtccg
     3061 agtactgcgc ccttcctggc atagtgcgtg gccagcgcac tgccgatgcc ggacgacgca
     3121 ccggtgatga agaccttcag tgcggcggaa tgcacgggtc ggcgagcatg gctccggcca
     3181 aagaaagcta cttcttcagt tttcgttctg cgcgcacctt gttgatgatt ttgtcagtca
     3241 gcggcagaag ttctgcggtc gacttaacgc tctggccaac gaccagataa cggccgtcta
     3301 ccgccaacgc gggaacacca cgaatcctga aattctgggc catctgatcg gcgcgcttgg
     3361 tcttgctgac cacaccaaag gagttgtagg cgtcggaaaa cttcttggca tcaacacctt
     3421 gagacactgc ccattcctgg atgctatcgt cgtcgatcag tctcaaaccc ttgtcgtgca
     3481 gggcatgaaa gacctctcca tcaagcttgg cgagatcacc ggtcgcttca agtgcgtagt
     3541 agagccgggc aagattggcc cactgtggac gattaaagct gaccggcaca cgcttgaaag
     3601 taacgtcacc cggcagtttg gacgcccact tgctgacatg cgggtgaaat tcgttgcagt
     3661 gtgagcaggc gtaggagaaa aactcgacga cctcaattcg gcccggatca tcggtccctt
     3721 gtgccggctc gatcacggtg tagtctcgtc cgacggcgag ttgggcatgc gccggcaggg
     3781 ccatcgaaac cagcgcgagt acgacggcag caaaccatcc cgtaatgaac gttttcatcc
     3841 tttgcattct cttctcctca atcctttctg accagactcg cctctatgcc ctgcttggca
     3901 agatcagaac gcatgttatt gacttcgtcc atcttcctga atggcccgag tcggacacga
     3961 taccagaccc tgtcctgaag catgacctgc tgaaccgacg cttctacgcc cattaacgcg
     4021 agtttcgtct tctggttgtc agcgtcatcg acattctgga acgagcccgt ctgcaaatag
     4081 accgactcct tgagcatgtt ttccttttca cccttgtcct tgactacttc ggtgggtttc
     4141 ggatcgggga tggcctcggc gttgccaggc aagatcttgt agaaatcgaa acgcggcttg
     4201 tcaccattct gcggaacagg atcacccggc ttgccgggca gcgccagcgg ggcaactgta
     4261 gcgccggctt gctgagcagc agaaggtgag ggctggccct tgttggaaaa gggtggcggc
     4321 gttttgaaga tgtaccagac cacacccgct gcacagagta cgccaagaac cagaccgagg
     4381 aatatgccga ccagcatgcc gccgcccgat ttccttctgg ccgttgcttt gcttggtttg
     4441 cgaggtttca tgtcacgact catgggcatc tatccggttc aatgttacat cgattggggg
     4501 gcgctcacac cgagaatgga caagccgtta cggatgactt gacgcacggc gacagccaga
     4561 gcaatacgcg cgttcgtcaa ggcgtcgtcg tcgaccagca ttcgttctgc attgtagtag
     4621 ctgtgaaatt caccggccag atccttcagg tagaaggcga tcaggtgcgg cgccagttcc
     4681 ccagctgccg attcgatgac ttccggaaac tcgctcaatc ttgcggcaag cgcctgttca
     4741 tgaggagtgt caaggcgcgc gaggtcggca tttccaaggg agctcccttc atcatcggca
     4801 ttgtcgttcc attgagcgag caaggagcag atgcgggcat gagcgtactg gatgtaatag
     4861 accgggtttt cattggactg gcttgtggcg agatcgaggt cgaagtcgag gtgctgatct
     4921 gatttgcgca gcacgtagaa aaatcggcag gcatcattgc ctacgtcctc gcgcagggtg
     4981 cgcaaggtga cgaattcgcc ggagcgtgtc gacatcgatg ctttctggcc attgcggtag
     5041 agaacggcaa actgcacgag ggccacgtcg aggagattcg ggtcgaggcc cagcgcagcc
     5101 agtgcgcccc tgacacgcgg gatgtagcca tggtggtcgg caccccagat attgatgagg
     5161 cgctcgaagc cgcgttcgta cttgttgaga tggtaggcaa tgtctgaagc gaagtacgta
     5221 taaagtccgt tgtcacgctg cacgacacga tccttctcgt cgccgaacgc cgtcgacttg
     5281 aaccacaggg cgccatcctg cgtgtaaagg tggcccgttt tttccagccg agcgacgcag
     5341 cgtgcaacga ggccggtatc gaacagcgat ttctccgaga accagacatc aaagcgcacg
     5401 ccgaattcct cgaggtcgcc gcgaccatca gcgagctgtt cggtcagcac gtggttgtga
     5461 acatagttcc agtcctcacc gagcaacgtt ttggcgttcg cgatcaacgc gtcgaggtgc
     5521 gactcgcact gcgccttggc gtcatcgtcg gtccggccaa ggtctggcag gccgggtgtg
     5581 ccgcggagca cagtggcagc gggatggcaa tacttgtcgt cgtgagcgag cttcatctgc
     5641 gcggccattt cgcgcacgta gtcgccctga taggcattcg gtggaaaggc gacgcaaccc
     5701 aaagtatcta cgtacagttc gagataacgc agccacgccg atagggccag aatatccatc
     5761 tggcggcccg cgtcgttcac gtaatactcg cgagtcacat cccagccggc gaagctcagc
     5821 aggttcgaca ggctggcgcc gtaggccgcg ccccggccat gacccacgtg cagtggcccc
     5881 gtcgggttgg cggacacgaa ttcgatctgc actttttttc ggccaccgag cgttgagcgg
     5941 ccgaatgact cgccctgcac aagaatgctg cgaacgactt ccaattttgc cgacggcagc
     6001 agatggaagt tgatgaagcc ggctccggcg atctcggtgc tgctgacgaa aggcgatttt
     6061 ggcaattcgg agcgcagcaa ttgagccagt tcacgtggat ttcgcttcat cgaacgcgcc
     6121 agttgcatcg ccagagggca agaaaaatcg ccgtgcgagg actgcttcgg gcgttccaga
     6181 atgatgttgg cgtccgcatg ctccggtacg acgctaatca gcgcagcgcg catcagttcg
     6241 gcgagatggg ccttgatagt gtgggtcatt gcggtgcttt gtcttgagaa tagggcaaga
     6301 ggcagattat acgatgccat gccgttcaag aggtcgcaca gaacggtcga tgagcgtcgg
     6361 ctgatatgtc gctctgcggg acaatagctg ccagcgaccg cactttcatg tcggacgcga
     6421 ggcggacgaa agcagaccta ttggccgaaa taacgtccag ggcgatgatt gactgtcagc
     6481 acgatgttga gcaccagcac gctcagaatc gataacacaa cccgttcttc gtcaacaacg
     6541 aacaaggcac cgcagacgat gatcgcgtcg gcgatcatct gcaaaattcc cgcacgccag
     6601 ccatggcgtt gttgcaggta gatggccagg ataccaatgc cgcccacgca gacttgatgg
     6661 cgaatcagca tcagtaaacc ggttccagcc aacaaaccgc caaggatggc agcaaatact
     6721 ggatcaacgc tttcaatgtt gatccagtga ggaaaccatt cgctatagaa cgaaatcagg
     6781 gccaccgcgc aaaaggtttt cagcgaaaac agcgtaccaa acgccttgaa cgccagagcg
     6841 tagaaaggta tgttgatcag aaagtaaagc gtaccgaaag gaatgccact cgcgtagtgg
     6901 gctagaaagg tcaatccagg ggtgcctccg gtcaacaagc cggcctgccg aaacagcacg
     6961 atcgcgaagg ccaccagcaa agtgccggtc agcaaggcct gaatatcttc gaacacgttg
     7021 tgataggggt tgacgttcat ggcacgcagt accgagagtg acgtccgagc ttagtccaga
     7081 gccatgaata cggcaaggtg cataatgccc gtgtctttcc gagaggtagt cacttgtcgt
     7141 tttaagacct tacccggtgc agcattccgt attcattagc agcaatcgaa ccagaagaag
     7201 gagcccaggc catgaaagta aaaaccttgg atggtcgtga atacgacctg cagcaggcca
     7261 ctctcgacag tctcaggatg cgtctcaagg gatcggtact tgcgaccggt gatgcgggat
     7321 acgacgaatc acgaaccgtc tggaacgcca tgatcgatca gacaccggcg ctcgtcgtgc
     7381 gttgtctcgg caccgccgac gtgatcgcct gtgtgcagtt cgctcgcgac aaccggcttc
     7441 tgctttgcat caagggcgga ggacacaaca tcgcgggtct ggcaacggtc gacggcgccc
     7501 tgatgctcga cctgtcgttg atgcgtggcg tctgggtcga tgcgcagcgg cagatcgctc
     7561 atgcgcaggc gggttgcctg ctcggcgacg tggatcgtga aacgcagctt cacgggcttg
     7621 ccaccgtcct cgggttcgtc tcgatgaccg gaattgccgg cctcacactg ggcggcggat
     7681 tcggctacct gacgcggcgc tgggggtgga cgaccgacaa cgtgatcggt atggacgtcg
     7741 ttaccgccga cgcaggtctg gtgcgggcga ccagtgacga aaacccggat ctcttctggg
     7801 gcttgcgagg cggtggcggc aacttcggcg tcgtgaccgg aatcgattac gcgctacacc
     7861 ctgtcggccc ggaagtcgtc ggcggaatcg tcgcatgggc tgccgacaat gctcccgagg
     7921 tgctcaggct gtaccgggcg ctggccgagg cggcaccgcc cgaacttgcg ctcgtgacgt
     7981 ttatgcgccc tgcgcctcca gcaccctggt taccgaagga catgcacggc aagccgatcg
     8041 tcgcgatcct tgcctgtcat tgcgggaacc cggaagaagg cgagaaggct gtcgccgcca
     8101 tcaagtcatt cggtactcct atcggcgacg tgctggtgcg tcggccatac acgcaactac
     8161 aggcactgct cgatgggaca cagccgaagg gccgacgcta ctactggaag agcgaatacc
     8221 tggcgcagat cgacgcgggg ttgtgcgaga agttcatcgc gcactcggca aaaatccgct
     8281 cacctcactc ggcaatgatc ctgttccaga tcggcggcgc ccttaaccgg tttgaggatg
     8341 aacattcccc cgttggaaac cgcgatgcgc gctatgtgct caacatcagc ggcgcatggg
     8401 agcaggcaac cgacgacgac tcgaacatcg ggtgggcgcg agaggcctgg gacgacctga
     8461 aagcgttctc gaccggtggc acctacatca acttcctgac ccaggacgaa ggcgccgagc
     8521 gtatcgaggc ggccctcggc aagaacatgt ccaggcttgc cgaggtcaag ttgcgctggg
     8581 atcccgagaa cgtatttcgc acgaaccgta atatcaaacc ggcctgagat aagggaatct
     8641 tcgcattccc gaatctcggc gcataatgct cctgtctttc caagaggact ggtttgtgat
     8701 tttgcgaaat ctcaagagtg gatacgccgc atgaaggcag cggcgcctgt tcgtcaactg
     8761 gtttccggtg aattcgaagt ccattcactc gaagac
//
LOCUS       DAORPM010000088         8739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1946_length_8739_cov_11.608591, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000088 DAORPM010000000
VERSION     DAORPM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1946_length_8739_cov_11.608591"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLD53_05825"
     CDS             <1..833
                     /locus_tag="PLD53_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HRC60464.1"
                     /translation="ELGQDYLKAGLLDRAEEVFNKLRDSAMVKEAKRNLLEIYQLEKD
                     WEKAIAIAAELPDIATQKEIAEYYCELAAAEMIRSRRDVASGYLKTALERNRKCVRAS
                     LLQGDIEAQQDRIEEAVACWQRIEQQDPTYLALVASRLLDAFRKLARRDEGLQLLRGY
                     LEHYHSLDLLDVVFQLVLESEGADAAYCLVRDELKRNPTLLGFDKLIEARLLVAPPET
                     RADLDLAKSIVQGYTRRLARYRCDNCGFKARQFYWRCPACGGWETYSPKRTEEFDLTP
                     "
     gene            852..1784
                     /gene="rfaE1"
                     /locus_tag="PLD53_05830"
     CDS             852..1784
                     /gene="rfaE1"
                     /locus_tag="PLD53_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HRC60465.1"
                     /translation="MVLGDFSTVRLLIVGDVMLDRYWFGEVSRISPEAPVPIVRVERS
                     EERPGGAANVARNAAALGAQVSLLALVGNDEPGERLRGLMKESDINASLYIDEAVTTT
                     VKLRVIGRQQQLLRIDFETIPSHEVLRAKLSEFEQRLPMCDAVVFSDYGKGGLTHISE
                     MISLARASGKIVLVDPKGEEYSKYAGATIITPNRGELRSVVGRWKDEAELERKASALR
                     TELALQALLVTRSDEGMSLFHAGGTVHEKAVAREVFDVSGAGDTVIATLAVMLARGAD
                     WTHAIHVANVAAGIVVGKLGTAVVTRAELDAALN"
     gene            1893..2888
                     /gene="rfaD"
                     /locus_tag="PLD53_05835"
     CDS             1893..2888
                     /gene="rfaD"
                     /locus_tag="PLD53_05835"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRC60466.1"
                     /translation="MYTIVTGAAGFIGSNLVKALNERGTDQIIAVDNLTRADKFKNLV
                     DCEIVDYIDKTEFLARLLGGQFNGQVDAIFHEGACSDTMESDGRYMMENNFRYSLGIL
                     DWCIDQGVQYLYASSAATYGGSSEFREERAFEAPLNVYGYSKFLFDQIVRQRLPKASS
                     QIVGFRYFNVYGPRESHKGRMASVAFHHFNQFRAEHKVRLFEGCNGFANGEQKRDFVF
                     VGDVAAVNLYFLDHPEKSGIFNVGTGRAQSFNELAAANVNSCRALEGEPAQSLDELVR
                     LGFIEYIPFPEALRGKYQSFTQADLATLRKAGYDAPFASVEQGVAKYVEWLNARV"
     gene            2881..3780
                     /gene="cysM"
                     /locus_tag="PLD53_05840"
     CDS             2881..3780
                     /gene="cysM"
                     /locus_tag="PLD53_05840"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HRC60467.1"
                     /translation="MYKTLEDFVGGTPLVRLKRLPDEGLEARGNVILAKLEGNNPAGS
                     VKDRPALSMIRRAEQRGEIKPGDTLIEATSGNTGIALAMAAAMGGYRLLLVMPENQSI
                     ERRQTMRAFGAELVLTPKSGGMELARDVAEKMQKEGSGLILDQFANPDNPLAHYEGTG
                     PEIWRDTQGRVTHFVSSMGTTGTIMGVSRYLKEQNPAVCIVGCQPEEGSQIPGIRKWP
                     EAYLPKFFEPSRVDRVESVAQGDAEAMTRRLAREEGIFAGISSGGAVVVSLRLAREIE
                     NATIVTIICDRGDRYLSTGVFPS"
     gene            3797..4585
                     /locus_tag="PLD53_05845"
     CDS             3797..4585
                     /locus_tag="PLD53_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC60468.1"
                     /translation="MKPVLVFDIETIPDVPGLRRLHDLDPALSDAEVAEMAFQQCRVK
                     SGNDFLPHHLQRIAVISCALRSGEDIRVWSLAEPEQSEAEIIQRFFDGVEKFTPQIVS
                     WNGGGFDLPVLHYRGLIHGVVAPRYWDLGDGDYGDSREFKWNNYISRYHTRHLDLMDL
                     LAMYQPRASVPLDALAKLIGFPGKLGMDGSKVWEAWQNGEISGIRDYCETDVMNTYLV
                     SLRFRLMRGELSRAEYDAEIRFVRSELERLDKPHWREFLAAWGG"
     gene            4725..4982
                     /locus_tag="PLD53_05850"
     CDS             4725..4982
                     /locus_tag="PLD53_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60469.1"
                     /translation="MLNLTLNTNDSIETVLPTVELAMHTGDVCNIHNINYLGHIHMAA
                     LTLLAMSENLLDPVTGRIFHPHPGFRLLGIDEHGVTRTLVM"
     gene            5222..6832
                     /locus_tag="PLD53_05855"
     CDS             5222..6832
                     /locus_tag="PLD53_05855"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRC60470.1"
                     /translation="MPLKIAIAQINATVGDLTGNAARILDFAEQAKAQGADLLLTPEL
                     ALCGYPPEDLLLREDFYVACERELDALAARVKGIAVIVGHPLLREGCRYNAATLLMNG
                     VRIATYCKQRLPNYEVFDEERYFESGDTACVVNINGVRCGINICADVWEAGAADLARE
                     SGAEILLVLNASPYHIGKQQRRADVLRKRIASTGLPVVYANLVGGQDELVFDGGSFAM
                     DSRGVLCCQLPKFDEALAMVDFDHGEPQPGIIVADQAVEAEVYQALVIGVRDYLGKNG
                     FPGAIIGLSGGIDSALTLCIAVDALGADKVRAVMMPSPYTAEISLSDSREMIRLLGVR
                     YDEIPIEPAMQTFASMLEKEFSGLPADTTEENLQARIRGMILMALSNKTGSLVLTTGN
                     KSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLSRWRNSVSCAIPERIITRAPSAELK
                     PGQTDQDNLPPYDVLDAIIEAYMEDDLSPREIIARGYAEADVRRVVQLLKISEYKRRQ
                     APVGIRVTQRGFGKDWRYPITNRYRDPY"
     gene            6893..7231
                     /locus_tag="PLD53_05860"
     CDS             6893..7231
                     /locus_tag="PLD53_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRC60471.1"
                     /translation="MKKIEAVIKPFKLDEVREALSEIGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIVVADDAVEGAIESIIKAARTGKIGDGKIFVSPVEQVVRI
                     RTGETGESAV"
     gene            complement(7365..7922)
                     /locus_tag="PLD53_05865"
     CDS             complement(7365..7922)
                     /locus_tag="PLD53_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRC60472.1"
                     /translation="MTQRRQPTPEDLAAFRAAVDGAVPLRVPERVAFEPPKPSPRPRQ
                     RELDEAAAVTESLYGPFEIDDLLAIGDADSYLRTGLPRNVLRDLRRGRWSIQNHTDLH
                     GLNRHEAHEEVSRFLAESHSSGKRCLRIVHGRGHGSPGREGILRQLVKSWLARRKDVL
                     AFCHAPPCDGGEGALWVLLKADFRG"
     gene            complement(8030..>8739)
                     /locus_tag="PLD53_05870"
     CDS             complement(8030..>8739)
                     /locus_tag="PLD53_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC60473.1"
                     /translation="HIHTAKLTEKPFTLIGDAGTYTCDALIISTGASAQYLGLPSEEA
                     FSGRGVSACATCDGFFYRNQPVAVIGGGNTAVEEALYLANIASHVTLVHRRDKLRGEK
                     ILQDKLFEKEKAGKVTIKWNNTLDEVLGDKSGVTGIRIKSTLNGACEDIPLMGVFIAI
                     GHKPNTDLFVGQLEMEGGYIITECGSKGNATATSIPGVFAAGDVQDHIYRQACTSAGT
                     GCMAALDAERYLDSLEN"
BASE COUNT         1793 a         2358 c         2668 g         1920 t
ORIGIN      
        1 ccgaactggg gcaggattat ctgaaagccg gtctgctcga ccgtgccgag gaagtattca
       61 ataagcttcg tgactcggcg atggtcaagg aagccaagag gaatcttctc gaaatctacc
      121 agctcgaaaa ggactgggag aaggcgatag ccatcgcagc cgaattgcct gacatcgcta
      181 cgcagaagga aatcgccgag tactattgcg agcttgccgc cgctgaaatg atccgttcgc
      241 gacgcgacgt tgcgtcgggc tacctgaaga cagcgcttga gcgtaaccgg aaatgtgttc
      301 gtgccagtct gttgcaaggt gacatcgagg cacagcaaga cagaatcgag gaggctgttg
      361 cctgctggca acgcatcgag cagcaggacc cgacctatct ggcgctggtt gcgtcccgtc
      421 tgcttgatgc attccgcaag ctggcgcgtc gcgacgaagg tttgcagctt ctgcgtggct
      481 acctggagca ttatcattcg ctcgacctgc ttgacgtcgt ctttcagctc gtgctggaaa
      541 gtgaaggcgc cgacgcggcg tattgtctgg tccgcgacga gttgaaacgc aacccgacac
      601 tgctcggatt cgacaagttg atcgaagccc gattgctggt cgcaccgccc gaaacgcgcg
      661 ctgatctgga tcttgccaaa agcattgtgc agggctacac acgccggctg gcacgctatc
      721 ggtgcgataa ttgcggcttc aaggcacgac agttctattg gcgctgtcca gcgtgtggcg
      781 gctgggagac ctactcgcca aaacgtaccg aagaattcga tttgacgcct tagtttgccg
      841 gggtaatttg catggttttg ggagattttt cgacagttcg cctgctcatt gtcggcgatg
      901 tcatgctcga ccgttactgg ttcggagagg tgtcgcgcat ttcgcctgaa gcgccggtgc
      961 cgatcgtcag ggtcgagcgt agcgaggaac gtcccggagg ggccgccaac gtggcacgca
     1021 atgcagcggc gcttggagca caggtctcct tgttggcgct ggtcggtaat gatgaaccgg
     1081 gcgaacggct aagaggcttg atgaaagaaa gcgacatcaa cgccagcctg tacatcgacg
     1141 aagccgttac gacaacggtc aagctgcgcg tgattggccg acagcaacaa ctgttgagaa
     1201 tagatttcga gacgatacct tcgcatgaag tgctgcgggc aaagctttca gagttcgagc
     1261 agcgcctgcc catgtgtgat gccgttgttt tctctgatta tggcaagggt ggattgacgc
     1321 acatcagcga gatgatcagt ttggcgcgtg cttccggaaa gatcgttctg gttgacccga
     1381 agggtgaaga gtactcgaag tatgcgggcg caacgatcat tacgccgaat cgaggtgagc
     1441 tgcgttctgt cgtcgggcgc tggaaggacg aggctgagct ggagcgcaaa gccagtgctc
     1501 tgcgcacaga actggcgctc caggcactgc tggttacgcg cagcgatgag ggcatgtcgc
     1561 ttttccatgc cgggggtacg gtccatgaaa aggccgtggc acgcgaggtg tttgatgtgt
     1621 cgggcgcggg tgatacggta attgccacgc tggcggtcat gctggcgcgc ggtgctgatt
     1681 ggacgcacgc gattcatgtt gcgaatgtcg cggccggcat tgtagtcggc aagttgggta
     1741 ctgccgttgt gacgcgtgcc gagcttgacg cggcactgaa ctgagggcgt tttatgtata
     1801 ccattgtcac aggcgcggca ggttttggct ccggcataac gcccgccact gcgggcatta
     1861 gccttctctg aaacctgccg aggagaatat tcttgtatac gatcgtcacc ggcgcggcag
     1921 gttttatcgg ctcaaatctc gtcaaggcgc tcaatgagcg cggtacagac cagattattg
     1981 cggtcgacaa tcttacgcgc gccgacaaat tcaaaaatct ggttgattgc gaaatcgtcg
     2041 attacatcga caagaccgag ttcctcgcac gactgctggg cgggcaattc aacggtcagg
     2101 tcgacgccat attccatgaa ggcgcgtgtt ccgacacgat ggagtctgac ggtcgctaca
     2161 tgatggagaa caatttccgt tattcactcg ggatactcga ttggtgcatc gaccagggcg
     2221 tgcaatacct ctatgcatca agtgcagcga cctacggggg aagcagtgaa ttcagggaag
     2281 agcgggcatt cgaggcgccg ctcaacgttt acggctattc caagttcctc tttgatcaga
     2341 ttgtgcgtca gcgtttgccg aaggcttcgt cgcagatcgt cggttttcgc tatttcaatg
     2401 tctatggtcc gcgcgaatcg cacaaagggc gtatggcctc ggtcgccttt caccacttca
     2461 accagtttcg tgccgagcac aaggtcaggc tgttcgaagg ctgcaatggt tttgccaatg
     2521 gcgagcagaa gcgtgatttc gtttttgtcg gcgatgtggc cgcagtgaac ctttacttcc
     2581 tcgatcaccc tgaaaaatcc gggatattca acgtcggcac cggacgcgcg caaagcttca
     2641 acgaactggc cgcagccaac gtgaacagtt gccgcgcgct ggagggtgag cccgcacagt
     2701 cgctggatga actcgtcagg ctgggcttca ttgaatacat accctttccc gaagcgcttc
     2761 gaggcaagta ccagagcttc actcaggcgg acctggccac gctccggaag gcgggttatg
     2821 acgcaccgtt tgccagtgtc gagcaaggtg tggcgaaata cgttgaatgg ttgaacgcgc
     2881 gtgtataaaa cgctggaaga tttcgttggc ggcacgccgc tggttcgctt gaagcgtctg
     2941 ccagatgagg gtctggaggc gaggggcaac gtcatcctgg ccaagcttga aggcaacaac
     3001 ccggcagggt cggtcaagga cagaccggcg ttgtcgatga tccgtcgcgc cgaacaacgt
     3061 ggcgagatca aaccgggcga tacgctgatc gaggcaacct cgggcaatac cggtatcgct
     3121 ctggcgatgg cggcggcaat gggcggctat cgcctgcttc tcgtcatgcc ggaaaaccag
     3181 agtatcgaac gtcgccagac catgcgtgcc ttcggtgcgg aacttgtcct cacaccaaag
     3241 agcggcggca tggagttggc ccgcgatgtg gcggagaaga tgcagaaaga aggcagtggg
     3301 ttgattctcg accagtttgc caacccggat aatccgctgg cacactacga aggcaccgga
     3361 cctgagatct ggcgcgatac gcaagggcgc gttacgcact tcgtcagcag catgggcact
     3421 acggggacga ttatgggcgt ttctcgctat ctcaaggagc agaatccggc agtctgcatt
     3481 gtcggctgcc aaccggagga gggttcgcag ataccaggaa tccggaagtg gccggaagcc
     3541 tatcttccga agttctttga gccatcgcgt gtggaccgcg ttgagtcggt cgcgcagggg
     3601 gatgccgagg ccatgacacg gcggttggca cgcgaagagg gaatttttgc cggtatttct
     3661 tccggtggcg cagttgtggt ttccttgcgg ctggcgcgtg aaatcgagaa cgccacgatc
     3721 gtgacgatca tttgcgaccg tggtgatcgc tacctgtcca ccggcgtgtt cccatcctga
     3781 acccatcgag tttcgtatga agcccgttct cgtcttcgat attgaaacca ttccggacgt
     3841 gccgggtctg cgccgtttgc atgacctcga cccggcattg tcggacgccg aggtcgcgga
     3901 aatggccttt cagcaatgtc gggtcaagag cggaaacgat tttctgccac accatctgca
     3961 acgcatcgcc gtcatctcct gcgcattgcg ttctggcgag gatattcgcg tctggtcgct
     4021 ggccgagcct gagcagagcg aagccgagat tattcaacgc ttcttcgacg gtgtcgaaaa
     4081 gtttaccccg cagatcgttt cctggaacgg gggagggttc gacctgccgg tgctgcatta
     4141 tcgtgggctg attcacggtg ttgtggcgcc gcgctactgg gatcttggcg acggcgacta
     4201 cggcgacagc cgcgagttca agtggaacaa ctatatcagc cgttaccaca cgcgccatct
     4261 cgacctcatg gatttgttgg ccatgtacca gccgcgagcc agcgtcccgc tggatgctct
     4321 cgccaagctg atcggtttcc ccggcaagct cggcatggat ggaagcaagg tctgggaggc
     4381 ctggcagaac ggcgaaattt ccgggattcg cgactactgc gaaaccgatg tgatgaatac
     4441 ctatctcgtg tcgctgcgtt tccggctgat gcgcggcgag ttatcgcggg ctgaatacga
     4501 tgccgagatc aggtttgttc gcagcgagct ggagcggctc gacaagccgc actggcggga
     4561 attcctggcc gcttggggcg gctgatgtcg cggtgctcgg gcggtacctt ccgtgagtac
     4621 atcgacaggc ttgctaaagt tgttgcgatg gaggccgtag accgacttgt ctccaacccg
     4681 aacctcacga ggtccagcgc cctaaaaaga tactgggaag tgccatgttg aatttaaccc
     4741 tgaataccaa tgactccatc gaaaccgttc tgccgaccgt tgaactggcc atgcataccg
     4801 gggatgtctg caacattcac aacatcaact acctgggcca tatccacatg gcggcactga
     4861 ccctgctggc gatgtccgaa aatctgttgg atccggtgac cggccggatc tttcacccgc
     4921 acccgggttt ccgcttgctt ggcatcgacg aacatggggt gacgcgaaca ctggtgatgt
     4981 gatctgaaca gtggtctggg cgtgcttttc ttcggacccg gtcgccatga atatggcaca
     5041 gggcgcagcc cgcatttcca tgcatcatga ccatctggtt gatttgcagt gtcgactcag
     5101 caattgcctg cgtataaccg tatacctcgc aacacgatgc gtggatcggt ttggcattga
     5161 cggctaggaa gtagtcgttg gtccgttctt taggcgggcg ctatcgttta tagttacgcc
     5221 tatgccccta aagatcgcca ttgctcaaat caacgccacg gtcggcgatc tgaccggcaa
     5281 tgcggcgcgg attctcgatt tcgccgaaca ggccaaggct caaggcgccg atcttctgct
     5341 tacccccgaa ttggcacttt gcggctatcc gccggaagac ttgttgttgc gcgaggactt
     5401 ttatgtggcc tgtgaacggg aactcgatgc actggcagcc agagtcaaag gcattgccgt
     5461 aatcgttgga catccgcttt tgcgcgaagg gtgccgctac aatgcggcca ccttgctgat
     5521 gaatggtgtg cgcatcgcga cttactgcaa gcagcgcttg cccaactatg aggttttcga
     5581 cgaggagcgc tatttcgagt ccggagatac cgcttgcgtc gtgaacatca acggagtccg
     5641 ctgcggcatc aacatctgtg ccgatgtctg ggaggcgggg gcggcggatc tggctcggga
     5701 atccggggcc gaaatacttc tcgtgctcaa cgcctcgccc tatcacattg gcaaacagca
     5761 gcgtcgagcc gatgtgttgc ggaagcgcat cgcgtcaacc ggattgccgg tggtttatgc
     5821 caatctggtc ggtggtcagg atgaactggt cttcgacggt gggtcgtttg ccatggattc
     5881 gcgcggtgtg ctgtgttgtc agttgcccaa gttcgacgaa gcgctggcaa tggttgattt
     5941 cgatcatggc gagccgcagc cgggcattat cgttgcggat caggccgtcg aagccgaagt
     6001 gtatcaggcg cttgtcatcg gcgtgcgcga ctacctcgga aagaatggtt tcccgggcgc
     6061 aatcatcgga ctttcgggcg gcatcgattc ggcgttgact ctttgcatcg cagtcgacgc
     6121 gcttggtgca gacaaggtac gtgccgtgat gatgccttcg ccatacaccg ccgagatcag
     6181 tttgtcggat tcgcgggaga tgattcgcct gctcggcgtg cgctatgatg aaattcccat
     6241 tgagccggcg atgcagacct ttgccagcat gctggagaaa gaattttccg gcttgccagc
     6301 cgacacgacg gaagagaacc tgcaagcgcg cattcgcggc atgatcctga tggcgctctc
     6361 gaacaagacc gggtcgctgg tattgacgac cggtaacaag tccgagatgg cagtcggata
     6421 ttgcacgctc tacggcgaca tggctggcgg gtttgcggta atcaaggaca ttgccaagac
     6481 gctggtttat cgtctgtcac gctggcggaa ttcggtttcc tgcgcgattc ccgagcgcat
     6541 cattacgcga gcgccttcgg ctgaattgaa gcccggtcag actgatcagg acaatctgcc
     6601 gccctacgat gtccttgacg ccatcatcga ggcgtatatg gaagacgacc tcagtccgcg
     6661 tgagattatt gccagaggtt atgccgaagc cgatgtgcgc cgtgtcgtac aactgctcaa
     6721 gatcagcgag tacaagcgac gccaggcacc ggtgggtatc cgcgtaacgc agcgaggttt
     6781 tggcaaagat tggcggtatc cgattacgaa tcgctatcgc gacccgtact gaactcatgc
     6841 tgaaacccga tttctggtac gatttggcaa gatttttccg gagtaaactg ctatgaaaaa
     6901 aattgaggct gtgatcaaac ccttcaaact cgacgaggtg cgcgaagcgt tgtcggaaat
     6961 tggcgtatcg ggcctgacag tgactgaagt gaaaggcttc ggccgtcaga agggccatac
     7021 cgagttgtat cgtggcgcag agtacgtcgt cgatttcttg cccaaggtca agatcgagat
     7081 cgttgtcgcg gatgatgcgg tcgaaggcgc cattgaatca atcatcaaag ccgcacgcac
     7141 cggcaagatc ggtgacggca agatcttcgt ctctcccgtt gaacaggttg tacgtattcg
     7201 taccggcgag acgggcgaat cagccgtttg attttcctcc cgttcgacac agacggctcg
     7261 caaattcgcg agccgttttt ggttcggccg gcaggagtat aagccggcat acctttcgaa
     7321 aactacgcca tccccaagcg attattccga ttctcagccg attctcagcc ccgaaaatct
     7381 gccttgagca acacccacaa cgcaccttcg ccgccgtcgc agggcggggc gtggcagaag
     7441 gccaaaacat ccttgcgacg cgcgagccac gatttgacca gttgacgcag gatgccctcc
     7501 cggccggggg agccgtgtcc gcgaccatgc acaatgcgca ggcatcgttt gccggagctg
     7561 tgtgattcag caagaaagcg cgaaacttct tcatgtgcct cgtgtcggtt gaggccgtgc
     7621 aggtcggtgt gattctggat cgaccagcgg ccgcggcgca aatcgcgtag aacgttgcgc
     7681 ggcagccccg tgcgcaggta ggagtcggca tcgccgatgg ccagcagatc gtcgatttca
     7741 aacgggccgt acagggattc ggtgacggct gccgcctcat cgagctccct ttggcgcgga
     7801 cgcggactgg gcttgggcgg ttcaaaggca acacgctcag gtacgcgcaa ggggacagcg
     7861 ccatcgaccg cagcacgaaa ggcggccaga tcttccgggg tcggttggcg gcgttgagtc
     7921 atcggaattg actagtgaac agaatatcca ccacaaagca tcctgagcac accgggaaag
     7981 cctggctgtc tatcctggca atccccgtat ttcttgcgct ttgtggctct cagttctcaa
     8041 gactgtcgag atagcgttcg gcgtcgagtg cggccatgca gccggtgccg gccgaggtgc
     8101 aggcttgtcg gtaaatgtga tcctgcacgt cgccggcagc gaagacaccg gggatcgaag
     8161 tcgccgttgc attccccttg ctgccacact cggtaatgat gtagccgcct tccatctcca
     8221 gttgcccgac aaagaggtcc gtgttcggct tgtggccgat ggcgatgaat acgcccatca
     8281 gcgggatatc ttcgcaggcg ccgttcagcg tactcttgat gcggattccc gtaacccctg
     8341 atttgtcgcc gagtacctcg tcgagcgtgt tgttccattt gatggtgact tttccggctt
     8401 tttctttctc aaagagtttg tcctgcagaa tcttttctcc gcgcagcttg tcgcgccgat
     8461 gtacgagcgt cacgtgactc gcgatgttgg ccagataaag tgcttcctcg acagcggtat
     8521 tgcccccgcc aataacggcc actggctggt tccggtagaa aaagccgtca caggtggcgc
     8581 aagcggaaac tccccggccg gaaaacgctt cctcggaagg caggccgagg tattgcgccg
     8641 aagcgccggt agagataatc agcgcgtcgc aggtgtaggt tccggcgtcg ccaatcagcg
     8701 tgaagggttt ctcggtcagc ttggccgtat ggatgtggt
//
LOCUS       DAORPM010000089         8725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1951_length_8725_cov_11.845329, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000089 DAORPM010000000
VERSION     DAORPM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1951_length_8725_cov_11.845329"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLD53_05875"
     CDS             <1..266
                     /locus_tag="PLD53_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60474.1"
                     /translation="PNWTIKEQALSEDTFHLALRAKSFRTGGDGEAIQIVKRRARQLQ
                     REKGYADYRILDYSEGIESSTPLTHRVSEGTIQLVKTVIPEGR"
     gene            complement(288..1067)
                     /gene="fliR"
                     /locus_tag="PLD53_05880"
     CDS             complement(288..1067)
                     /gene="fliR"
                     /locus_tag="PLD53_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRC60475.1"
                     /translation="MISLSTAELNAWIVAFFFPLARVLALLVAAPPFNNPAVTVRVKL
                     LLGLAITIAIAPTLQQIPVAEPASGMGLLILAQQIVIGFAMGFSMRLVFSAVDMAGMM
                     ISNQMGLGFATAYDPASASQTPVISEFLGVLALLIFMAINGHLMVIATLGKSFAVLPI
                     GAGAAASSTWLNIASAGGIIFSSGVLLALPLVVAMLITNIALGILVRVAPQLNLLAIG
                     FPVTILLGFSSLLLSLSYLSMPIQQMFEFGLQSMLGYFVIK"
     gene            complement(1075..1344)
                     /gene="fliQ"
                     /locus_tag="PLD53_05885"
     CDS             complement(1075..1344)
                     /gene="fliQ"
                     /locus_tag="PLD53_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRC60476.1"
                     /translation="MTPELVMNIGRQAIEMMLILAGPLLLSALFIGLIISIFQAATQI
                     NEQTLSFIPKLVGIFLVLILAGPWMLQMMVDYIRRLFESIPQMIG"
     gene            complement(1341..2105)
                     /gene="fliP"
                     /locus_tag="PLD53_05890"
     CDS             complement(1341..2105)
                     /gene="fliP"
                     /locus_tag="PLD53_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608787.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRC60477.1"
                     /translation="MKARGLIRWIAPLALLLLLLSPLATWAQTGGLPALTSTPSAGGG
                     QTYTLSIQTLITLTALTFIPAALLMMTSFTRIIIVLSLLRHALGTQTSPPNQVLVGLA
                     LFLTFFIMSPVLDKVYVDAYQPLSEDRISFVQAVELGAVPLRGFMLKQTREADIALFA
                     RMANLPKLESANDVPMRVLIPAFVTSELKTGFQIGFIIFIPFLIIDMVVASVLMSMGM
                     MMMSPVMIALPFKLMLFVLVDGWHLLLGSLVMSFAT"
     gene            complement(2102..2404)
                     /gene="fliO"
                     /locus_tag="PLD53_05895"
     CDS             complement(2102..2404)
                     /gene="fliO"
                     /locus_tag="PLD53_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRC60478.1"
                     /translation="MLIGLTVIIGILFFGAYFLRKLNGGRSFGNAGPMRIVGGLMISV
                     RERIVLVEVEDTWIVIGIVPGQIKTLLTLPKGEIPAANSDEKPFGKWLKQMTERNK"
     gene            complement(2635..3060)
                     /gene="fliN"
                     /locus_tag="PLD53_05900"
     CDS             complement(2635..3060)
                     /gene="fliN"
                     /locus_tag="PLD53_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRC60479.1"
                     /translation="MTDDTENSETGSAAEDDWAAAMAEQAIAEAAPVAQAQTAQIFPA
                     FGETDGKASMMNELDMILDIPVQMAVELGRTKITIKNLLQLAHGSVVELDAMAGEPMS
                     VFVNGTLIAQGEVVVVNDKFGIRLTDIITPSERMRKIGR"
     gene            complement(3053..4057)
                     /gene="fliM"
                     /locus_tag="PLD53_05905"
     CDS             complement(3053..4057)
                     /gene="fliM"
                     /locus_tag="PLD53_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRC60480.1"
                     /translation="MATDFLSQDEVDALLKGVTGETDQAETSEDDEAGARSYNLGTQE
                     RIVRGRMPTLELINERFARYLRIGLFNFMHRSTDISVGPIRVQKYSEFIRNLVVPTNL
                     NLVAAKPLRGTSLFVLDPNLVFLVVDNMFGGDGRFHTRVEGRDFTPTEQRIIQGLLGV
                     VFTEYARSWKPVFDISFEYIRSEMNSQFANIATPSEIVISTTFSLEFGGTVADMHICF
                     PYSMIEPIRDLLYSTMQSDQLSTDQRWIVMLRNQLKNAEVEIAAQLATTTVTLNQILK
                     MKSGDIIPISIPDVIIANVDDVPLMQCRYGQQGGQYALKIERFITPESEDVALGVEHD
                     "
     gene            complement(4064..4606)
                     /locus_tag="PLD53_05910"
     CDS             complement(4064..4606)
                     /locus_tag="PLD53_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRC60481.1"
                     /translation="MAKEAKPEAEVAEAAPPKKKSKKLLIIIAIVVVLVLVIGGAAAF
                     LLLKARSAHDGEDGATSAEMEKSGKKGKEVIPVYVALDAFTVNLVPENGDQFLQLMIS
                     VEVEDAHVGEKLNMYAPKIRNNVMLLLSGKKAAELMTKEGKETLANEVRNLMNGILDP
                     YANKGEGPVREVLFTSFIIQ"
     gene            complement(4721..5740)
                     /locus_tag="PLD53_05915"
     CDS             complement(4721..5740)
                     /locus_tag="PLD53_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRC60482.1"
                     /translation="MSVTIVSTLPNQTQATAADSVAGDDNSVAGLDFASLLFGQLAPI
                     VAEALPNAVARTPLPATEPAATDDASLIAGLVVAPQEAGLNGSVSLPDAGKIDKASTD
                     SISALQVPLAAGANPKPEGKTGALGSESEISQAPTVDNKPAKFAVDPLASTRAEQVVS
                     GKTSADALPNSAMALTGNTPGIAGNLPAQSTVSLPIPTPVRDQAWAGDFSQKIMWLTT
                     NNKHTAQLSLNPPQMGPIEVSVNLDKGNAVVSFVSANGDTRQAIESALPKLREMFATA
                     GIELGQTNVSAQSSGQHAGGWEGGRNSSAGMADRAILVADSAGALQGRAFSVSRGNGL
                     IDIFA"
     gene            complement(5851..6306)
                     /gene="fliJ"
                     /locus_tag="PLD53_05920"
     CDS             complement(5851..6306)
                     /gene="fliJ"
                     /locus_tag="PLD53_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRC60483.1"
                     /translation="MTKPFALQIVLELMQVRADDATHRLAQLISSERNAKSKLDMLRQ
                     YREEYAVRFKQAAQNGLAQREWHNYQEFLNRLDEAIDSQLQTVALQIQNTAEGQTHWQ
                     QQRTKLKAFDTLSERHYVNETNLELKREQKTQDEFAARGKTDDDSPQTE"
     gene            complement(6303..7760)
                     /gene="fliI"
                     /locus_tag="PLD53_05925"
     CDS             complement(6303..7760)
                     /gene="fliI"
                     /locus_tag="PLD53_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HRC60484.1"
                     /translation="MTNHETRAPMNDVFANEHLAGWSGFLDNCREAASLATPLLPSGH
                     LTRINGLVMEASGLKLPLGSSCRILPVGGSPIEAEVVGFNGERLFLMPTEDVYGLSPG
                     AKVVALETPTAPPRMGQPPPARRRAIDRAKLLPVGNELLGRIIDGAGRPLDKYGPLTA
                     DTLRPLQSRPINPMSRAPIEQPLDVGVRAINALLTVGRGQRMGLFSGSGIGKSVLLGM
                     MARYTTAEIIVVGLIGERGREVKEFIEQILGEEGLRRSVVIAAPADVSPLLRLQGAAY
                     ATSIAEYFRDQGRHVLLIMDSLTRYAMAQREIALAIGEPPVTRGYPPSVFARLPQLVE
                     RAGNGADAGGSITAFYTVLTEGDDPQDPIADAARAILDGHVVLSRTLADAGHYPAIDI
                     EQSISRAMVNLIPPDHLDQIRHFKQLYSRYQRSRDLISVGAYAAGSDPLLDQAIKLYP
                     AFEGFLQQSLSEKAEFSTSEEQLYALFATQPRGLE"
     gene            complement(7747..8439)
                     /locus_tag="PLD53_05930"
     CDS             complement(7747..8439)
                     /locus_tag="PLD53_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HRC60485.1"
                     /translation="MTGFVPKEMLTAYQRWEVAAFDEERQEGERAEESRSAQGNDNRS
                     TETADQTEQTPVALPTAAEIERMHIEAHQQGFASGHAEGLAEARAGAAKINALMTSLQ
                     QSLRGLDQHVADQLLATALEVASQVLRQSLRVKPELLLPVVKEAVAILHPHSGHPALV
                     VHPDDAELIRTHLGDQLAHNNWRIIEDTTLSAGGCRVELGASEVDATLETRWRRVIES
                     IGINQDWLDDKP"
     gene            complement(8453..>8725)
                     /locus_tag="PLD53_05935"
     CDS             complement(8453..>8725)
                     /locus_tag="PLD53_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HRC60486.1"
                     /translation="DDRGIQLLLREIQSDSLILAMKGASEPLREKIFKNMSQRAAEML
                     REDLESRGPVRLSEVEGEQKEILKIVRRLADEGQIVLGGAGGEAMI"
BASE COUNT         1896 a         2361 c         2517 g         1951 t
ORIGIN      
        1 cacccaactg gactatcaag gagcaggctt tgagtgaaga cacctttcat ctcgctttgc
       61 gtgccaagag ctttcgcacc ggaggggatg gcgaggctat ccagatcgtg aagcgacgag
      121 cgcggcagct tcagcgcgaa aaaggttacg cggactatcg catactcgat tattcggaag
      181 gaattgagtc gagcaccccg ctgacgcacc gcgtttccga gggcacgatt cagctagtca
      241 aaaccgtaat tccggagggg cgctagctag tgcatttcaa gatggcgcta tttgattaca
      301 aagtagccca gcattgactg caatccgaac tcgaacattt gttgaattgg catactgagg
      361 tacgacaggc tgagcaggag cgaggaaaac cccagcagaa ttgtcacagg aaaaccaatc
      421 gccagaagat tgagttgtgg tgcaacacgt acgagtatcc ccaaggcaat attggtgatc
      481 agcattgcca ctacaagcgg gagtgccaac aaaaccccgg acgaaaagat tattccccct
      541 gcgctggcga tgttcaacca ggtgctgctt gcggctgctc cagcacctat cggaagtacc
      601 gcaaaacttt tccccagcgt ggcaatgacc atgagatggc cgttgattgc catgaatatg
      661 agcaatgcaa gtacgccaag gaactccgat ataacgggag tctgggaagc actcgccggg
      721 tcgtatgcgg tcgcgaatcc gagccccatc tggttgctga tcatcatgcc cgccatgtca
      781 actgcgctga agacaagacg catggagaac cccatggcga aaccaatgac tatttgttga
      841 gcgagaatca ggagacccat tccggaagca ggttcggcaa cgggaatttg ttgcaaagta
      901 ggcgcgatgg cgatggtgat ggcaaggccg agcaatagct tgactcgaac cgtcacggct
      961 ggattgttga acggtggcgc tgcgaccagc aacgccagga cgcgcgccaa cgggaaaaaa
     1021 aaggcaacga tccaagcatt gagttcagca gtactcaggc tgatcatgag cagttcagcc
     1081 gatcatctgt ggaatactct cgaacagccg tcggatgtag tcgaccatca tctgtaacat
     1141 ccagggcccg gccagaatca gcaccaggaa gatccccacc agctttggaa taaacgacag
     1201 cgtttgctca ttaatctgcg ttgctgcttg aaagatactg atgatcaggc cgataaacag
     1261 tgccgacagc aacagcggcc ccgcgagaat caacatcatc tcgatagcct gacggccaat
     1321 attcatgacc agttcagggg tcatgtggcg aagctcatga ccagtgagcc cagcaacaga
     1381 tgccagccat cgaccagaac aaaaagcatc agcttgaatg gcaacgctat catcaccggc
     1441 gacatcatca tcattcccat ggacataagc acgctggcga ccaccatatc gatgatcagg
     1501 aacggaatga aaatgatgaa cccgatctgg aatccggtct tcaattcact ggtgacaaag
     1561 gccggaatca ggacgcgcat ggggacatca ttggccgact caagcttcgg caggtttgcc
     1621 attcgggcaa agagagcgat gtcagcttcc cgggtttgct taagcataaa gccacgcagg
     1681 ggtacagcgc cgagttcgac cgcttgcaca aagctgatcc ggtcttccga aagcggttgg
     1741 taggcgtcaa cgtaaacctt gtccagcacc ggtgacatga taaaaaacgt caagaagagc
     1801 gcgaggccaa ccagcacctg attcgggggt gacgtttgag tgcccagcgc gtgtcgaagc
     1861 aaagatagca cgatgatgat gcgggtaaaa ctggtcatca tcagcagtgc ggccgggatg
     1921 aatgtaagcg cagtgagcgt gatcagcgtc tgtatggaga gcgtgtaggt ctggccgcca
     1981 cctgcgcttg gcgtactggt cagtgcaggc agtccgccgg tctgagccca cgtcgctagt
     2041 ggagagagca gcaggagaag cagggccagc ggggcaatcc agcgaatgag gcccctggct
     2101 ttcatttgtt gcgctcggtc atttgcttta accacttgcc aaaaggtttt tcgtcgctgt
     2161 tggctgccgg gatctcgccc ttgggaagtg taagcagcgt tttgatctgc cctgggacaa
     2221 tgccgatcac aatccaggtg tcttcaactt caacgaggac tatgcgctcc cggacactga
     2281 tcatcaaacc gcctacgatt cgcatggggc cagcgttgcc aaagcttctg cctccattga
     2341 gcttgcgcag gaagtaagcg ccaaagaaca ggatcccgat tattaccgtc aggccgatga
     2401 gcatttgaat cagggccgta ccggacacgc caggcgtctc gactgcggcg ttttgcgcaa
     2461 acgagagatc ggaaagcaaa gcaagcgcca taaagcaagg gccgcttatg cgaaggggaa
     2521 gaaatccggg atgtcgaatc aagtcagtcg gccatggata cactctggcc gctatcttaa
     2581 tgcatgccgg ctgctggtga aactctgatg cgggctataa ctccgctgaa agtttcaacg
     2641 cccgatcttc cgcatgcgct cggaaggtgt gatgatgtcg gtgagacgga taccgaattt
     2701 gtcattgacg acaaccactt caccttgagc aatgagtgtt ccattgacga agacgctcat
     2761 cggttcgccg gccattgcgt caagttcaac caccgagccg tgagccagtt gaagaaggtt
     2821 cttgatggtg attttcgttc gaccgagttc gacagccatc tgaaccggga tgtccagaat
     2881 catgtcgagt tcgttcatca tcgatgcttt gccgtctgtt tccccaaagg caggaaatat
     2941 ttgcgctgtt tgtgcctgcg ctaccggagc tgcctcggca atggcctgtt cggccattgc
     3001 tgcggcccaa tcgtcttcgg cggcgctccc ggtttcgctg ttttcggtat cgtcagtcat
     3061 gctctactcc cagagcgaca tcctcgctct ctggtgttat gaatcgttcg atcttgaggg
     3121 cgtattgacc gccctgctga ccgtagcggc attgcatcag tggaacatca tcgacattgg
     3181 cgatgatcac gtccggaatg ctgatcggaa tgatgtcgcc ggacttcatt ttgaggatct
     3241 gattgagtgt gactgtcgtt gtagcgagtt gagcagcgat ttcgacctcc gcatttttca
     3301 gctgattacg tagcatgacg atccagcgtt ggtcggttga taactggtcg ctttgcattg
     3361 tgctatagag cagatcacga atcggttcga tcatcgagta ggggaaacag atgtgcatat
     3421 cggcaaccgt gccgccaaac tccagcgaga atgtcgttga gataacgatt tcggacgggg
     3481 tggcgatatt ggcaaactgc gagttcatct cggagcgaat gtattcgaag gagatatcga
     3541 atacgggttt ccatgagcgt gcgtattctg tgaacacaac gcccagaagc ccctgaatga
     3601 tgcgttgttc ggttggtgtg aaatcgcggc cctcgacacg cgtgtggaaa cggccgtcac
     3661 caccaaacat gttatcgacg acgagaaata ccaggttggg atcaagtaca aagagcgatg
     3721 tgccacgcag cggtttggcg gcgaccaggt tcagattggt cggcaccacc agattgcgga
     3781 tgaattcact gtatttctga acgcggattg gaccgaccga tatgtcggtc gagcgatgca
     3841 tgaagttgaa caaaccaata cgaagatagc gggcgaaccg ttcgttgatg agttcaagcg
     3901 tcggcatgcg cccgcggacg atgcgctcct gagttcccag attgtaggat cgggcaccag
     3961 cctcatcgtc ctcggaggtt tcagcctggt ccgtctcacc ggtgacaccc ttgagtagag
     4021 cgtcaacctc gtcctgggag agaaagtcgg tggccattgt gtgctactgg attatgaaag
     4081 aggtaaacag cacttccctg accgggcctt cgcccttgtt ggcgtaggga tcgagaatgc
     4141 cattcatcaa attgcgaacc tcgttggcca gggtttcctt gccttctttg gtcatcagtt
     4201 cagcggcttt tttcccggac agaagcagca tcacattgtt tcgtattttt ggcgcgtaca
     4261 tgttgagttt ttcgccgaca tgcgcatcct cgacctcgac cgaaatcatc agttgcagaa
     4321 actggtcccc attctccgga acaagattca ctgtaaacgc gtcaagcgcg acatagaccg
     4381 gaatgacttc cttgcccttc ttgccggatt tttccatttc ggcggaagtg gctccatctt
     4441 cgccgtcatg tgcgctacgt gccttgagca agaggaaggc ggcggcacca ccaatcacca
     4501 gaaccagcac aacgacaatg gcgatgatga tcagcagctt tttgctcttc ttctttggcg
     4561 gcgctgcttc tgcgacctct gcttcaggct tggcttcttt ggccatccgc tattcctccc
     4621 tccgattttc agtgttccca agtttccggc tgaatcgtgt tgctcatcgc tatagattct
     4681 gcattctgcc atgctgcagg cagagcgccg ggcatggagt tcaggcgaaa atatcgatca
     4741 gtccatttcc ccgcgataca gaaaacgctc ttccttgcag cgcgccagcc gagtctgcca
     4801 caagtatagc cctatcggcc atccccgcgg atgagttccg gcctccctcc cagcccccgg
     4861 cgtgctgtcc ggatgactgc gcgctgacat tggtctgtcc caactcgata ccggcagtgg
     4921 cgaacatttc ccgcagtttt ggtagcgccg attcgatggc ttgccgggta tctccgtttg
     4981 ctgaaacgaa ggacaccacc gcgttaccct tgtccaggtt aaccgatacc tctatcgggc
     5041 ccatttgtgg ggggttcagg ctgagttggg cagtgtgctt gttgttcgtg gttagccaca
     5101 tgatcttctg actgaagtcc ccagcccagg cttgatcacg gactggcgtt ggaatgggca
     5161 gcgagactgt gctttgtgcc gggagatttc ctgcgatacc aggtgtgtta cccgtcaaag
     5221 ccatggcaga attcggaaga gcgtcggccg aagtcttgcc cgaaacgact tgctccgcac
     5281 gtgtcgaagc aagtgggtcg acggcaaatt ttgccggttt gttatcaacg gttggtgctt
     5341 gggatatttc ggactcggac ccgagtgccc cggttttgcc ttcgggctta ggattcgctc
     5401 cagcagccaa gggtacttgc agtgccgata tcgagtcagt gcttgccttg tcgatcttgc
     5461 cggcatcagg aagggaaaca gacccgttca gcccagcctc ctgtggggca accaccagcc
     5521 cggcaatgag tgaggcgtcg tcggtcgctg cgggctcggt tgcgggcaag ggagtacgcg
     5581 caacggcgtt cgggagggcc tcggcaacga tcggtgccaa ttgaccgaac agtaagctag
     5641 cgaagtccag tccggccacg gaattgtcgt cgccagcgac gctgtcggca gccgtggctt
     5701 gcgtctggtt gggcagcgtt gaaactatag taactgacat ggacaatcct tgagggcacg
     5761 tgttgcgtca agtaacgcaa tatttatgcc agccagaagt ttgtggcctc tttgtaaggg
     5821 aatgccgtga ccaagccgat tgaagctttg ttattcggtt tgcggtgaat cgtcatcggt
     5881 cttgccgcgt gcggcaaatt catcctgggt cttctgctcc cgctttagct ccagattggt
     5941 ttcgttgacg tagtggcgtt cggaaagcgt atcgaaagcc ttgagtttcg ttcgttgttg
     6001 ctgccaatgc gtctggcctt ctgcggtgtt ttgtatctgc aaggcgacgg tctgtagctg
     6061 cgaatcgatg gcttcgtcaa gacggttgag aaattcctgg tagttgtgcc actcgcgctg
     6121 agccaatccg ttctgtgcgg cctgtttgaa gcgcaccgcg tattcctcgc gatactgccg
     6181 cagcatgtcc agtttgcttt tcgcgttgcg ctcactggaa atcaattgcg cgaggcgatg
     6241 tgttgcatca tcggctcgaa cttgcatcaa ttcgagtacg atttgcaggg cgaaaggttt
     6301 ggtcactcca gacctcgggg ttgagtagcg aacaaagcgt aaagctgttc ttcgctggtg
     6361 gagaactcgg ctttctcgct cagggactgc tgcaggaacc cttcgaacgc cgggtagagt
     6421 ttgatcgcct gatcaagcaa agggtcggag cctgcagcat aggcgccaac actgatcagg
     6481 tcgcgagagc gctggtagcg cgaatagagc tgcttgaagt ggcggatctg gtccaggtga
     6541 tcaggtggga tcagattgac catggcgcgt gagatcgatt gttcgatatc gatggccgga
     6601 taatgcccgg cgtcggccag cgtccgcgaa agaaccacgt gaccatcgag aatggcgcgt
     6661 gctgcatcgg ctatcggatc ctgcggatcg tcaccttcgg tgagcaccgt atagaaggcg
     6721 gtgatcgaac cacccgcatc ggcaccattg cccgcgcgct cgacaagttg gggaagccgg
     6781 gcaaaaaccg acggaggata gcctcgggtg accggcggct cgccaatggc cagtgcaatt
     6841 tcgcgttgag ccatggcgta gcgggtgagc gaatccatga tcagtaggac atgccggccc
     6901 tgatcgcgaa aatattcagc aatcgatgtg gcgtaggcgg caccctgcag acgaagcagc
     6961 ggactgacat ctgccggagc ggcgatgacg acggaccggc gcaggccttc ttcgccaaga
     7021 atctgttcga tgaactcctt cacttctcga ccgcgctcgc caatcaggcc tacgacaatg
     7081 atctcggccg tcgtataccg ggccatcatc ccgagcagga cgctcttgcc gattccggaa
     7141 ccggaaaaaa gtcccatgcg ttgtccacga ccaaccgtca gtagcgcgtt gatcgcgcga
     7201 acgccaacat caaggggttg ttcgatcggc gcgcgcgaca tcgggttgat aggacggctc
     7261 tgtagcgggc gcaaagtgtc cgccgtgagt gggccgtatt tgtctagcgg tcggccggca
     7321 ccatcaataa tccggccgag cagttcattg ccaacgggaa gcagtttggc gcgatcgatg
     7381 gctcgtcgcc gggcgggcgg gggttgtccc attcttggcg gagccgttgg tgtttccagc
     7441 gcaacgacct tggcgccggg cgacaagcca tacacgtcct cggtcggcat aagaaacaag
     7501 cgctcaccgt tgaatccgac tacctccgcc tcgattggcg acccgccgac aggcaaaatg
     7561 cggcacgaac tgccaagcgg caatttgagg cctgaagctt ccatgaccag tccgttaatt
     7621 cgtgtcagat ggccgcttgg cagcagtggt gtggccaggc tggctgcctc gcggcagtta
     7681 tcgagaaaac cgctccagcc tgccagatgc tcgttagcaa agacatcgtt cattggagca
     7741 cgcgtttcat ggtttgtcat cgagccagtc ctggttgatt ccgatggact cgatcacgcg
     7801 tcgccaacgt gtttccaggg tggcatcaac ttcacttgcg ccgagttcaa cccggcatcc
     7861 gccagcgctc aacgtggtgt cttcgataat gcgccagttg ttatgtgcga gctgatcgcc
     7921 aaggtgggtg cgaatcagtt cggcatcgtc aggatggacg acaagcgccg ggtgtccgga
     7981 atgcggatgg aggatggcga ccgcctcctt gactacgggg agcagtagct ccggcttgac
     8041 gcgcaggctc tggcgcaata cctgactggc gacttccagt gcggtagcca gcaactggtc
     8101 ggcgacgtgt tgatcaagtc ccctcagcga ttgttgcagg cttgtcatga gggcattgat
     8161 ctttgcagca ccggctcgcg cctcagcgag gccttccgca tggccggatg caaaaccctg
     8221 ctggtgcgcc tcgatatgca tacgctcaat ttcggccgcc gtcggcaggg caaccggtgt
     8281 ttgttcggtc tgatccgcag tttcggtcga tctgttgtcg tttccctgag ccgatctgga
     8341 ttcctcagcg cgttcgcctt cctgcctttc ctcgtcaaat gcggcaacct cccagcgctg
     8401 ataggcggtg agcatctctt tgggaacaaa gccggtcatc agcgcagcgt actcaaatca
     8461 tcgcttcgcc acccgcgccg ccaagcacga tctggccttc gtctgccaga cggcggacga
     8521 tcttgagaat ttccttctgt tcgccctcga cttcggagag gcgcaccgga ccgcgcgatt
     8581 cgagatcctc gcgcagcatt tcagcagcac gttgagacat gttcttgaat atcttctcgc
     8641 gcaatggctc ggacgcgccc ttcatcgcca gaatcagcga gtcggactga atttcgcgca
     8701 ggagcaactg gatgccgcgg tcgtc
//
LOCUS       DAORPM010000090         8575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_1997_length_8575_cov_11.747535, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000090 DAORPM010000000
VERSION     DAORPM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1997_length_8575_cov_11.747535"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLD53_05940"
     CDS             <1..635
                     /locus_tag="PLD53_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRC60487.1"
                     /translation="FVLDCIASGAVWVDMVANNVDVLISAPQKGWSSSPCCALVALGE
                     RARDRIDSTTSSSFAVDLKKWLQIMEAFENGGHAYHATMPTDALAGLRDVMLETEAYG
                     FDRVCQEQKDLGRRVRELLVARGFPSVAAAGFEAPGVVVSYTTDPEIQSGKKFIRTGL
                     QTAAGVPLQCDEPADFRSFRIGLFGLEKLHQPERTLSILENALNQLGELE"
     gene            663..2312
                     /locus_tag="PLD53_05945"
     CDS             663..2312
                     /locus_tag="PLD53_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HRC60488.1"
                     /translation="MTRDNASHTSPPAGKRNKIVSAAEAVRRIRDGDTVATGGFVGIG
                     FAENIAVAIEQRFLGADPQSPAGTPRNLTLTYAAGQGDGKDRGLNHLGHEGLVGRVIG
                     GHWGLAPKLQKLAVNNLIEAYNLPQGIITHLFRDIAAGKPGLLSRVGLGTFVDPRHGG
                     GKMNTITKRDLIEVMVIDGEEYLFYKTFPIHVGIVRGTTADPDGNITMEKEALTLEAL
                     AIAMAAHNSGGIVIAQVERIAERGTLNSRQVKIPGILVDCVVVSEKPEYQMQTFIETY
                     NAAFSGELRVPMSAIPAMEMDERKIIARRAAFELRPDVIVNLGIGMPEGVASVAAEER
                     IIDLMTLTAEPGVVGGIPAGGLSFGAATNAEAIIDQPSQFDFYDGGGLDLAFLGLAQA
                     DRHGNLNVSKFGTRLSGAGGFINISQNAKRLVFVGTFTAGQLSVGIADGALQILQEGQ
                     TAKFVPEVEHRTFSGEYAFRREQPVLYITERCVFRLVADGLELIEIAPGVDLERDILA
                     QMTFRPAISPQLKTMDARIFRDEPMDIRDDILSAASGDGAR"
     gene            2353..3180
                     /locus_tag="PLD53_05950"
     CDS             2353..3180
                     /locus_tag="PLD53_05950"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: protein motif:HMM:NF007821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HRC60489.1"
                     /translation="MKYRLVALDLDGTLIDSQLQIRSETIEALSRVRAQGVQVMIVTG
                     RHHGAAYPYWRQLELELPAICCNGTYMYDFRAGRPLASNPYTMQEACSLLELIRQNGI
                     YTMIYADDFMAYERESLHVNKLLEWSATLPQDVRPRIDRVDSFEHMLEGCDTVWKFAT
                     AGDDLPIMSAFAANLERSLGLSCEWSAHNRLDISHAGNSKGKRLDQWIAEHGIAREEV
                     IAFGDQQNDVEMLRVAGLGVAMGNSRAEVRECADWVAGSNDSDAIANTLHRFVLTGG"
     gene            3312..>5057
                     /gene="tkt"
                     /locus_tag="PLD53_05955"
     CDS             3312..>5057
                     /gene="tkt"
                     /locus_tag="PLD53_05955"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRC60490.1"
                     /translation="MVSQASLANAIRVLAMDAVQAANSGHPGAPMGMAEIAVALWNRH
                     LKHNPADPKWPNRDRFVLSNGHGSMLLYALLHLTGYDLPLDELKRFRQLHSKTPGHPE
                     YGHTAGVETTTGPLGQGIGNTVGFALAERLLAAEFNREGFPIVDHYSYVFMGDGCMME
                     GISHEACSLAGTWKLAKIIALYDDNGISIDGHVDGWFTDDTPGRFEAYGWNVIRNVDG
                     HDIDAVDRAIRAAKSQNDKPTLICCKTVIGKGSPNKAGTHDVHGAPLGKDEIAATRAA
                     LGITEGEFEISADVRNGWNAREAGAKLQSEWQALFDAYRKAHPALAAEFERRTKGELN
                     SEFASKTAETLQDIAAKGEAIATRKSSQNAIQALAPLMPEFVGGSADLSGSNLTLWKG
                     CRTLHAEAMAEGGNYIHYGVREFGMAVVMNGMLLHGGLRPFGGTFLMFSEYARNAIRM
                     AALMRINPIYVFSHDSVGVGEDGPTHQPIEQTATLRMMPNLDVWRPCDSVETFVAWQI
                     AIEHRTTPTALILTRQNLPHQPRDAEQIKAIRRGGYVLTEAVGTPQAVIIATGSEVSL
                     AVAAQKSLAEKGIAAR"
     assembly_gap    5058..5157
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5158..5948
                     /locus_tag="PLD53_05960"
     CDS             <5158..5948
                     /locus_tag="PLD53_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRC60491.1"
                     /translation="LARLEGLDLRLIPSSGRLRTCTSIVEATGASGHRVTELVEPSST
                     VDKSCVIAMTKTVEDLLPSASALVVAGSRAPGYPAGYPARLARMATESGVPVILDMQG
                     PDLRDALIARPAVVKINLAEFVATFLADRFKVREHSGALAETDVSPDLTKAITEVSRN
                     FVTSFVLTRGAKSTLLIRDGSMRVLPVPPLTAEEVINPIGSGDTFLAGMLAQLLKTDW
                     PSTWESTPMEALEKAIEFATACAQSNARTARPGFLEGSFKAYSD"
     gene            complement(5974..6669)
                     /locus_tag="PLD53_05965"
     CDS             complement(5974..6669)
                     /locus_tag="PLD53_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3334 family protein"
                     /protein_id="HRC60492.1"
                     /translation="MSTPENPVVFGTEDILLSLCNSVTRVLTAATQSQIHFSGMVQRI
                     TKTCLKPDIGCFVLFDGGFSGLVVINFSSQAAMELYANYMLNMGMSKAELASSHTSDE
                     VSNVMGELMNQIVGDFTGKVSRELQTHITQNQPKMLVLSKQVMLSVDTNLDKPEARRV
                     TFYTGRNNIFYLELAIDRTEFIKLFDFEAPEDNDPDELIAQANAASAPGSARPPQPSQ
                     SKDQDDFLDSLGM"
     gene            complement(6803..7060)
                     /locus_tag="PLD53_05970"
     CDS             complement(6803..7060)
                     /locus_tag="PLD53_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HRC60493.1"
                     /translation="MNTTASAVGYAAAILTTISFVPQVLKVWRTRSARDVSLGMYSLF
                     TLGIFTWLVYGVLIESWPVILANFVTLVLAGMVLAMKLKFG"
     gene            7158..7958
                     /locus_tag="PLD53_05975"
     CDS             7158..7958
                     /locus_tag="PLD53_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC60494.1"
                     /translation="MSWWLAYIGLGLFAGFFAGLLGIGGGLVMVPMLAMMFAAQAVFP
                     PNEVLHLALGTSVATILFTSISSLRTHHQHGAVLWKVVIQITPGILFGTLLGTLFASK
                     VPAKPLALFFTAFVCLVALQLVLDLKPKASRELPGAGGVFAVGSGIGAVSALVAIGGG
                     SLTVPFLTWCNVRVQNAIGTSAAIGFPIAIGSSLGYIYNGWGHANLPPWSMGFVYLPA
                     LVWMVPASMLIAPLGARLAHRLPVSTLKRIFACVLIALAAKMLWNFFS"
     gene            8181..>8575
                     /locus_tag="PLD53_05980"
     CDS             8181..>8575
                     /locus_tag="PLD53_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC60495.1"
                     /translation="MSNESTVALLDSSSLDAWKKAATKSAPGGVVDAINWVTPEGIVV
                     KPLYTKQDVAGLQYTDTLPGFAPFVRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRK
                     ALAAGGQGVSVAFDLATHRGYDSDNARVVG"
BASE COUNT         1713 a         2597 c         2500 g         1665 t
ORIGIN      
        1 tgttcgtact cgattgcatc gcctccggcg ccgtctgggt cgatatggtc gccaataatg
       61 tcgacgtgct gatcagcgcg ccgcaaaaag gctggagcag ctcgccgtgt tgtgcgctgg
      121 tggcgcttgg cgagcgcgcc cgcgaccgga tcgacagcac gacaagcagc agttttgccg
      181 tggacctgaa gaaatggttg cagatcatgg aagctttcga aaacggcgga cacgcctacc
      241 acgcgacgat gcctaccgac gcgctcgccg gcttgcgcga cgtcatgctt gaaaccgaag
      301 cctatggctt tgaccgcgtg tgccaggaac agaaagatct cgggcgtcgg gtgcgtgaac
      361 tgctggttgc cagaggtttc ccgagcgttg cggccgcggg cttcgaggcg cccggtgtgg
      421 tggtgagcta taccaccgac ccggagatcc agtcggggaa gaaattcatc aggaccggac
      481 tccagaccgc cgccggcgtt ccgctgcagt gcgacgaacc cgcggacttt cgcagcttcc
      541 gcattggcct gttcgggctg gaaaaactgc atcaaccgga acgcacgctg agcattctgg
      601 aaaatgccct gaaccagctt ggcgaacttg agtaggctga aaggccagca aggaggaatg
      661 ctttgactcg cgacaacgcc tcgcacacct cgccaccggc gggcaaacgc aacaagatcg
      721 tcagcgcggc cgaggccgtg cgccggattc gcgatggcga tacggtcgcc accggcggct
      781 tcgtcggcat cggctttgcc gaaaacattg ccgtcgccat cgaacagcgc tttcttggag
      841 ccgacccgca gtccccggcg ggcacgccgc gcaacctgac gctgacctat gccgccggcc
      901 agggcgacgg caaggatcgc ggcctgaacc atctcggtca cgagggtctg gtcggccggg
      961 tgatcggcgg ccactggggg ctcgcgccca agctgcaaaa gcttgccgta aacaacctca
     1021 tcgaagccta taacctgccg cagggaatta ttacccacct gttccgcgac atcgccgccg
     1081 gcaagccggg attgctgtcg cgcgtcggtc tgggcacctt cgtcgatccg cgccatggcg
     1141 gtggcaagat gaacaccatt accaagcgcg acctgatcga agtgatggtc atcgacggtg
     1201 aggagtatct tttctacaag accttcccga tccatgtcgg catcgttcgc ggcacgaccg
     1261 ccgaccccga cggcaacatc accatggaga aggaagcact gacgctggaa gcactagcca
     1321 tcgccatggc cgcgcacaac tcgggcggaa tcgtgatcgc ccaggtcgaa cgcatcgccg
     1381 agcggggtac gctcaattcg cgtcaggtga agattcccgg aatcctggtc gattgcgtgg
     1441 tcgtctccga aaagcccgaa tatcagatgc agaccttcat cgaaacctac aatgccgctt
     1501 tttccggaga actccgggta ccgatgtcgg cgatcccggc gatggagatg gacgaacgca
     1561 agatcatcgc ccggcgcgcg gccttcgagc tcagacccga cgtcatcgtc aacctcggta
     1621 tcggcatgcc cgaaggcgtc gccagcgtcg ccgccgaaga aagaataatc gacctgatga
     1681 cgctgacggc cgagcccggc gtggtcggcg gcattccggc cggtggattg agcttcggtg
     1741 cggcaaccaa cgccgaagcg atcatcgacc agccctcgca gttcgatttc tacgatggcg
     1801 gcgggctgga tctcgccttc ctcggtctgg cacaggccga ccggcacggc aacctcaacg
     1861 tcagcaagtt cggcacgcgg ctgtccggcg ccggcggctt catcaacatc agccagaacg
     1921 ccaagcggct ggtctttgtc ggcaccttca ccgccggtca gctcagtgtc ggcatcgccg
     1981 acggcgccct ccagattctc caggaaggcc agacggcaaa gttcgtccca gaggtggaac
     2041 atcgcacatt cagcggcgag tacgccttca gacgcgaaca accggtgcta tacattaccg
     2101 agcgctgcgt cttccgcctg gtcgccgacg ggctggaact gatcgaaatc gcgcccggtg
     2161 tcgatctgga gcgcgacatc ctagcgcaga tgactttccg cccggcgatc agcccgcaac
     2221 tgaagacgat ggatgcgcga atcttcaggg atgagccgat ggacattcgc gacgatattc
     2281 tgtccgccgc gtccggcgat ggagcccgat gaagactcac ggacatcggc cggacagctc
     2341 ccggagaaat gcatgaagta tcgcctggtc gcgctggacc tcgacggaac attgatcgac
     2401 agccagttgc agattcgcag cgagacgatc gaagcgctga gtcgcgtgcg agcccagggc
     2461 gtgcaagtca tgatcgtaac cggccgccat catggcgcgg cttatcccta ctggcgccag
     2521 ctcgaacttg aactaccggc catctgctgc aacgggacgt atatgtacga ttttcgcgct
     2581 ggccgcccgc tggccagcaa tccctatacc atgcaggaag catgcagtct gcttgagctg
     2641 atccgccaga acggtatcta cacgatgatc tacgccgacg acttcatggc gtatgaacgg
     2701 gaaagcctcc acgtcaacaa gctcctcgaa tggtcggcca ccttgccgca ggacgtgcgt
     2761 ccgcgcatcg accgggtcga ctcgtttgag cacatgcttg aaggatgcga caccgtgtgg
     2821 aaattcgcca cggccggcga cgatctgccg atcatgagcg cctttgccgc aaacctcgaa
     2881 agaagtctcg gcctgagttg cgaatggtcc gcacacaacc gtctcgacat ctcacatgcc
     2941 ggcaatagca agggcaagcg tcttgatcaa tggatcgccg agcatggcat cgcgcgcgag
     3001 gaagtgattg cctttggcga ccagcagaac gatgtcgaaa tgctgcgcgt ggccggcctg
     3061 ggggtcgcga tgggcaacag ccgggcagag gttcgcgagt gtgccgactg ggtcgccggc
     3121 agcaacgaca gcgacgccat cgcgaacacc ttgcaccgtt tcgtactgac aggtggctag
     3181 catccagata aaatagcggg ttgcgatgga tcgactttcg ggcacgatgt cgcttgcagt
     3241 tgttctccga ctgcacccgt cacggcccgc ggtttgtcgg caaatatttc aatgaatcag
     3301 ggggtgagca aatggtttct caggcttcgc tggccaatgc aattcgtgtg ctggccatgg
     3361 atgcagtaca ggccgccaat tcggggcatc cgggcgctcc gatgggcatg gctgaaatcg
     3421 cggtcgcctt gtggaaccgc cacctcaaac ataatccggc cgatccgaaa tggcccaacc
     3481 gtgaccgatt cgtgctgtcc aatggccatg gctcgatgct gctttacgct ctgctgcatc
     3541 tgacgggcta cgatctgccg ctcgacgaac tcaaacgctt ccgccagttg cacagcaaga
     3601 cgccgggaca tccggaatac ggccatacgg cgggcgtcga aacgaccacc ggtccgctcg
     3661 gacagggcat cggcaacacc gttgggtttg ctctggccga acggctgctg gccgccgagt
     3721 tcaatcgcga gggtttcccg atcgtcgatc actacagcta cgtcttcatg ggcgacggct
     3781 gcatgatgga aggcatttcc cacgaagcgt gttcgctggc cggcacctgg aagctcgcga
     3841 agatcatcgc gctctacgac gacaacggca tctccatcga cggccacgtc gacggctggt
     3901 tcaccgacga tactccggga cgattcgaag cctatggctg gaacgtcatc cgcaacgtcg
     3961 atggccacga catcgacgcg gtggatcgcg ccataagggc ggcaaaatcg cagaacgaca
     4021 aaccgacact gatttgctgc aagacagtca tcggcaaggg atcgccgaac aaggccggca
     4081 cgcacgacgt gcacggcgct ccgctgggca aagacgaaat cgccgccaca cgcgccgcgc
     4141 tgggcatcac cgagggtgaa ttcgaaatat ccgccgacgt gcgcaacggt tggaacgccc
     4201 gcgaagccgg tgcaaaactc cagtccgaat ggcaagcctt gttcgacgcc tatcgcaagg
     4261 ctcacccggc gctggccgct gaattcgaac gccgcacgaa gggcgaactc aattccgaat
     4321 tcgcgtccaa gacggccgaa accctgcagg acattgctgc caagggtgaa gcgatcgcca
     4381 cacgcaagtc gtcgcagaac gccattcagg cgctggcccc gctgatgccg gagttcgtcg
     4441 gcggttccgc cgacctttcc ggctccaatc tcacattgtg gaaaggctgt cgaaccctgc
     4501 acgccgaagc catggccgaa ggcggcaatt acatccacta cggcgtgcgc gaattcggca
     4561 tggccgtggt catgaacggc atgctactgc acggcggcct gcgtcctttc ggcggcacct
     4621 tcctgatgtt ctccgaatac gcgcgaaacg ccatccgcat ggccgcgctg atgcgcatca
     4681 atccgatcta tgtcttctcg cacgactccg ttggtgtcgg cgaagacggc ccgacgcacc
     4741 agccgatcga gcagaccgcc accctgcgca tgatgccgaa cctcgatgtg tggcgcccct
     4801 gcgacagcgt ggaaaccttc gtcgcctggc agatcgccat cgaacaccgc accacgccga
     4861 ccgcgctgat cctgacgcgt caaaatcttc cccatcaacc tcgcgacgcc gagcagatca
     4921 aggccattcg ccgcggaggc tatgtactca cggaggccgt cggcacaccg caggcagtga
     4981 tcatcgccac cggctccgaa gtgagcctgg cggtagccgc ccagaaatcg cttgccgaaa
     5041 agggaatcgc cgctcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc
     5161 ttgcccggtt ggaaggactg gatctgcggc tcataccttc ctccggacgc ctgcgcacct
     5221 gcacgtcgat cgtggaagct acaggcgcat ccggacaccg ggtgaccgag ctcgtcgaac
     5281 cctcctctac ggtggataaa tcctgcgtta tcgccatgac caagaccgtt gaggacctgt
     5341 tgccgtcagc aagcgccctg gtcgttgccg gatccagggc gcccggctat ccggcggggt
     5401 accctgctcg tctggcccgt atggcaacgg aatccggagt gcccgtcatt ctggacatgc
     5461 agggccccga ccttcgtgac gccctcatcg cccgacctgc cgtagtcaag atcaatctcg
     5521 ccgaattcgt tgcgaccttt cttgctgacc ggttcaaggt ccgggaacac agcggcgcgc
     5581 tggctgaaac ggatgtttca cccgacctca cgaaggccat taccgaagtg tcgaggaatt
     5641 tcgtaacaag cttcgtcctg acccgcggcg ccaagagcac gctgctcatt cgggacggga
     5701 gtatgcgagt cctgccggtg ccccccctca ctgcggagga ggtcatcaac cctatcggtt
     5761 ccggcgacac ttttcttgcc ggcatgttgg cacagttgct gaagaccgac tggccgtcca
     5821 catgggaaag cactcccatg gaggcgctgg aaaaagcgat tgaatttgct actgcctgcg
     5881 cccagtccaa tgcgcgaacc gctcgccccg gattccttga agggtcattc aaggcttaca
     5941 gcgactagcc ctctgtcgga atcgacttgc cctttacatc ccgagggaat cgagaaagtc
     6001 gtcctggtct ttggactgcg acggctgggg tgggcgcgcg gagcctggtg ctgacgccgc
     6061 gttagcctgt gcaatcaact cgtctggatc gttgtcctcc ggcgcctcga agtcaaacag
     6121 cttgatgaat tcggtccggt cgatggccag ttccaggtag aaaatgttgt tgcgaccggt
     6181 gtagaaagtg acgcggcggg cttcgggttt atcgagattg gtatctacgc tgagcatcac
     6241 ctgcttggac aggacgagca tcttgggttg gttctgcgtg atgtgcgttt gcaattcacg
     6301 cgacaccttg ccggtgaagt cgccgacgat ctggttcatc agctccccca tgacgttgct
     6361 gacctcatcg gaggtgtgcg agctggccag ttctgctttc gacataccca tattcagcat
     6421 gtagttcgca tacagttcca tggccgcctg ggacgagaag ttgatcacca cgagaccgga
     6481 gaagcctccg tcgaacagta cgaagcagcc gatatccggc ttcaggcagg tcttggtgat
     6541 ccgctggacc atgccggaaa agtgaatctg gctctgggtg gccgcagtca gaaccctggt
     6601 cacagaattg cacagactca acaggatgtc ttcggtgcca aatacgacgg ggttttctgg
     6661 tgtactcatg attctgcaag gcccctgatc aggaagaatg gcgtatgtat atcatagctc
     6721 ccgactggat aggcatccga tgcaaaaatt gactgtaatt gcagaatacg gatacccgct
     6781 ttttgatgtt gaaacttcgt cgtcacccaa atttcagctt catggccaat accatacctg
     6841 ccagtaccag ggtgacgaag ttggccagaa ttaccggcca cgactcgatg agaacgccgt
     6901 aaacaagcca ggtgaagata cccagcgtaa acaaggaata catgccgagc gaaacgtctc
     6961 ttgccgagcg cgttcgccag acctttagaa cctgcggcac aaaggagata gttgtaagga
     7021 tggcggcggc gtagccgact gcggaagccg tggtattcat cgagcaccta ttttcgcgca
     7081 aattgtggat cagcccggcc gtcgcctgcg agtcatacgc cctggcaagc tacaatcccg
     7141 tctgaaggga gattcatgtg agctggtggt tagcgtatat cggactgggc ctttttgccg
     7201 ggttttttgc cggcctgctg gggattggcg gcggactggt gatggtgccg atgctggcca
     7261 tgatgtttgc cgcacaagca gtgttcccgc caaatgaagt attgcatctg gcgctcggca
     7321 catccgttgc gacgatcctg tttacgtcga tttcaagtct gcgcacgcac catcagcacg
     7381 gcgccgtgct ctggaaagtg gttatccaga ttacgccggg aatactgttc ggcacactgc
     7441 tgggaacgtt gtttgcgtct aaagtgccgg cgaaacccct agcgctgttt tttacagcct
     7501 ttgtctgtct ggtagccttg caactggttc tcgacctgaa acccaaagcc tcgcgtgagt
     7561 tgccgggggc cggcggcgtt tttgccgtcg gttcgggcat tggcgctgtg tcggcactgg
     7621 tagccatcgg cggcggctcg ttgaccgttc ccttcctgac ctggtgcaat gtgcgcgtac
     7681 aaaacgcgat cggaacctct gcggcgatag gcttcccgat cgccatcggc agttccctcg
     7741 gctacatcta caacggctgg ggccatgcca accttccgcc ctggagcatg ggattcgtgt
     7801 atttgccggc tttggtctgg atggttccgg cgagcatgct gattgctccg ctaggtgcga
     7861 ggctcgctca tcgcctgccc gtttcaaccc tgaaacgcat cttcgcctgc gtgctcattg
     7921 ccctggccgc gaagatgttg tggaactttt tttcctgatc tgaactcgac tacacgccat
     7981 gcggtggaac catgatttca atcaataaag acgctcagga accggtttct tcttgccttg
     8041 aagctgaggg taaagtagga ttgtccgttc gtggcgattt ggccaccgct ggtgagcgca
     8101 acgctcgcag ccaggtcgcg ttttctgcat ccggctgagg aacgtccccc caagtttctt
     8161 acattctgaa aggtcttgtc atgtccaacg agagcactgt cgcgctactt gattcttcaa
     8221 gtctagacgc ctggaaaaaa gcggctacca aatcggctcc aggcggagtg gttgatgcca
     8281 tcaactgggt caccccggaa ggcatcgtcg tcaagcccct ctataccaag caggatgttg
     8341 ccggcctgca atacaccgat acgcttcccg gtttcgcccc atttgttcgc ggcccgcagg
     8401 cgaccatgta tgccgtacgt ccctggacga tccgccagta cgccggcttt tcgaccgccg
     8461 aggagtccaa cgcgttctac cgcaaggcgc tggccgccgg tggtcagggc gtttcggtcg
     8521 ccttcgacct ggccacgcac cgcggttatg actccgacaa cgcccgcgtc gtcgg
//
LOCUS       DAORPM010000091         8522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2014_length_8522_cov_6.539743, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000091 DAORPM010000000
VERSION     DAORPM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2014_length_8522_cov_6.539743"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLD53_05985"
     CDS             <1..734
                     /locus_tag="PLD53_05985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRC60496.1"
                     /translation="LDFVLPDDHDYVEFVLKINRQNDAVHWLEFRLARTGLRCSAHCL
                     NLGGSDKPDQPMSGMIMVLTNATARSQQDERLWTLAHYDALTDLPNRILFWDRVEQAL
                     RHAKRLDSGAAMLWIDLDGFKGVNDHLGHAAGDALLQQVAQRLKGRIRDSDTLARMGG
                     DEFAVIMPGITASESASHVATELVASLAEPFDLPQGTANISGSIGIALYPQDADSVES
                     LTQCADMAMYTAKTSGKNQVQVWTG"
     gene            784..1905
                     /locus_tag="PLD53_05990"
     CDS             784..1905
                     /locus_tag="PLD53_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60497.1"
                     /translation="MATKSSLIGASLRLFCIFQTLLLTLIGSANAEDFPALNGSVVLE
                     LNNRTTWVGPNRAQRDDLYLFAEANTALQLTESWLIRAIVTLEPLIDPYPYKNRAFED
                     EDVRWKDVFIQYDNGVAGFRGGRITADFGFAWYGAPGLEATTLAEDYAVWDRMGASGW
                     YRLQSDIFGSTTVSASMYSLDTSLFSASWLNTRQQKTASRGGPSNTDSPTSFAVALYG
                     TDIPKLPGLSWQLALLRQQVDFVTDSSGQRVYSVADEKGASANLQQRIAFDNGIDSTT
                     VGEVVYLKDKGGIPGASGRYLIVGETLRYGRWRAQTALTTRWLDGASADSSRDRLFAL
                     TAGYDFTDRWSCDAVWRQAKVFGETADSVRVRLRYQLPF"
     gene            2173..>2899
                     /locus_tag="PLD53_05995"
     CDS             2173..>2899
                     /locus_tag="PLD53_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRC60498.1"
                     /translation="MNIRQRIILLIALTFGAIAAIGGFAILQSRNNAVAVRAVTEGVV
                     PSALAAGDLVASIKDVQLTAISMVSAADDNAAGRAAEILKQLQAQIAQSVDLQSQAAD
                     SDAQRGLIAQTQESLKNYFKSIDDTTRFKLAGQKEMAEAFLFANVAEYQTELSGIVET
                     LRIEKNRSKDAAILSLNDKLGQVVRGISLVTLVAVLVLGSVGSFLYLRITRPLKQMQD
                     AIETIRTNLDLTHRIPVAGNSEID"
     assembly_gap    2900..2909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3080..3805
                     /locus_tag="PLD53_06000"
     CDS             3080..3805
                     /locus_tag="PLD53_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRC60499.1"
                     /translation="MAASVQEMAVSVAHVSDSSAVAQGIAQGSLEKSKDGAETIERTV
                     TQMVVMAEDVHLTSQAMKELGRRSIEIGGITGTIKKIAELTNLLALNAAIEAARAGEH
                     GLGFAVVADEVRKLAERTARATREIEAVIGAVQNETKSAVDDMHQMASRVVANAGEAR
                     HAGESIVQIRNESKHVVEVSSEIATALKEQSSATELFAIQIEKIASMSEKNTAVTGEV
                     KEVSGDVKRLSSELYRLVARFQV"
     gene            3964..6612
                     /locus_tag="PLD53_06005"
     CDS             3964..6612
                     /locus_tag="PLD53_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC60500.1"
                     /translation="MTQTRRLRSLMIWSVVCANIAVFLLSGYSLYRSRLQYESRAETL
                     TQNAASAAAKNVANNIDKIDLALRTVADELERQTAGRGIDDKAMASFLAKQELRLPEV
                     EAFRVVNANGLVVLGDVFGRETDGKWTEHDSFHYHRDHADNQMRVATPLIDHSTNLYV
                     LDFTRRYESKDGSFAGIVAATVVVDNITLLLSGFGLGTHGTIYLRDASLALIARFPPI
                     PNKSMGQIGNRLVSETARKSIATGERSATYRAAAADGFERIYSFKRIERTSMLIGAGM
                     AREDYLAGWTEEAYKTLATAVLFLLLSQLFAAAFLRLLNAMGRESTRNEMYLQSASDG
                     VQIWNAKENRIVEVNNRLCEVLGYTRDELLALQFGEWAVGWTAEVLNGRKLPEMLAAS
                     TPATIEMRLRRKDGALVDVEVSISNFYLEDVQYVYASYRDIRERKQAEQRILSLAHFD
                     PLTHLPNRRLLIDRLSQALLASERSLEYGAMMILDLDNFKAINDTQGHDVGDRLLVEV
                     ARLVSACVRKEDTVARLGGDEYVVLVENLGRSEALASNQTEQIAEKIRNALNQAYRTT
                     PDGQPYQSTASIGMTLFLGQSDSTDDLLKQADVALYQAKGAGRNAIRFFESRMQELIE
                     ARLLMEAALRQGLQQGELHLFYQPQVDHEQRLIGAEALLRWLPPDKAPVSPMEFIPLA
                     EATGLIIPIGLWVMQTACAQLNEWSKNAAMCHLKLAINVSARQFRQPDFVEQLRESLE
                     RSGANPSLLKLELTESVVLEDLEDVINRMLQIKALGVTFSLDDFGTGFSSLSYLKRLP
                     LDQVKIDQSFVRDVATDANDAAIVRAIIAMSRSLGMEVIAEGVETNEQLTFLSVSGCP
                     NYQGYLFSRPLPLAQFETFVEQALRI"
     gene            complement(6640..7398)
                     /locus_tag="PLD53_06010"
     CDS             complement(6640..7398)
                     /locus_tag="PLD53_06010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRC60501.1"
                     /translation="MPWQADLFRSSEKPLAQNETPRRIALGDRIVPYVLRRAKRRTIG
                     LTIDQRGLRVGAPPRASLNEVESLIHRHGDWVIQKLDEWRSRLRPEPLAIVDGVQVPM
                     LGKNLQIRIATGNNRAVWNELADPVLTLCLRSPKDAAPQLEKALREKARLLFSQRLQH
                     FAARLDVPAPPLALSSARTRWGSCSLKTGIRLNWRLIHFSEDVIDYVVAHELAHLREM
                     NHGARFWRIVEQMYPECKRARAELKALAGAVPHW"
     gene            complement(7382..>7543)
                     /locus_tag="PLD53_06015"
     CDS             complement(7382..>7543)
                     /locus_tag="PLD53_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRC60502.1"
                     /translation="KHPGLITVSIGPAIDPAGKTAAEITTLAEQWIEGEMRRLSPHRY
                     PDTACAVAG"
     assembly_gap    7544..7553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7554..8148)
                     /locus_tag="PLD53_06020"
     CDS             complement(<7554..8148)
                     /locus_tag="PLD53_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC60503.1"
                     /translation="MAFLRSTLFLLLAILITAPFGLLVTLAVVLPIACRFQLIALWRA
                     GFMALCRYVLGLRYQVIGKENIPATPSVILSKHQSAWETVGLQAIFPPLVFVLKKSLL
                     MIPFLGWAFAAVKMISIDRSAGTDALRQVIDQGRDRLKAGYWVVIFPEGTRIAPGAKR
                     RFKPGGAHLAVSAGAPAVPVAHNAGEFWARNAFVKKSG"
     gene            complement(8148..>8522)
                     /locus_tag="PLD53_06025"
     CDS             complement(8148..>8522)
                     /locus_tag="PLD53_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRC60504.1"
                     /translation="IGRGLFDMDTLNAIHDKMHHAVQDAGGRINAIFYCPHTADSKCK
                     CRKPKTGMFERIGACFNADLSKAYVVGDALRDLNPASQLGARPILVLTGKGLITQRED
                     TLPEGTLVFADLSEAVEHILKS"
BASE COUNT         1839 a         2324 c         2563 g         1776 t
ORIGIN      
        1 tcctggactt tgtgcttccc gacgaccacg actacgttga attcgttctg aaaattaatc
       61 gccagaacga tgcggtgcac tggcttgagt ttcggctggc gcgcaccggg ctgcgctgct
      121 cggcgcactg cctgaacctt ggcggatccg ataaacccga tcagcccatg agcggcatga
      181 tcatggttct gaccaatgcc accgcacgca gccagcagga tgagcgactg tggactctgg
      241 ctcactacga cgccctgacc gatttgccca accgcatcct cttctgggat cgggtcgaac
      301 aggccttgcg tcacgccaaa cgcctcgata gcggtgcggc catgctgtgg attgatctcg
      361 atggtttcaa gggcgtaaac gatcatctcg gccatgcagc gggtgatgct ctgttgcagc
      421 aggtagcaca gcggctgaag ggccggatcc gtgacagtga caccctggcg cggatgggtg
      481 gcgatgaatt tgccgtgatc atgccgggaa tcacggcgtc cgagagtgca tcgcacgtcg
      541 ccaccgagct ggtcgccagc ctggccgagc cgtttgatct gccgcaggga acggcaaata
      601 tctccggcag tatcgggatt gcactgtatc cgcaagacgc cgacagcgtc gagtcgttga
      661 cgcaatgcgc cgatatggcg atgtacaccg ccaagacgtc cggaaagaat caggtacagg
      721 tttggacggg ttgatgaaca tggctcgact gagttggatc tcaatcgaaa catcgactct
      781 cagatggcaa cgaagagcag cctgatcggc gccagcttga gacttttctg tattttccaa
      841 acgcttcttc tgactcttat cggctccgcc aatgccgagg attttccggc gctcaatggc
      901 tcggtcgtac tcgagctgaa caacagaaca acgtgggtgg gaccgaaccg tgcgcaacgc
      961 gatgacctgt acctgtttgc cgaagcgaat accgcacttc agctgaccga aagctggctc
     1021 atccgcgcca tcgtcacgct cgaaccattg attgacccgt atccctacaa gaacagagcc
     1081 ttcgaagacg aggatgttcg ctggaaggat gtcttcattc aatacgacaa cggtgttgcc
     1141 gggtttcgtg gtggccggat cacggctgac tttggtttcg cctggtatgg cgcacccgga
     1201 ctcgaagcca cgacgttggc ggaagattat gccgtctggg acagaatggg tgcatcaggc
     1261 tggtatcggc tgcagagcga tatctttggc tcgactacgg tcagcgcttc aatgtactcg
     1321 ctggacacct cgctgttttc cgcctcgtgg ctgaacaccc gccagcaaaa aacggcgtcc
     1381 cggggtggcc caagcaatac cgactcgccg acgtcgttcg ccgttgcgct ctatggcacc
     1441 gacattccaa agctgccggg tctttcgtgg caactggccc tgctgcgcca acaagttgac
     1501 ttcgttaccg atagcagcgg gcagcgcgtt tactcggtcg ccgatgaaaa aggcgcttcc
     1561 gccaaccttc agcagcgaat cgcattcgat aacggaattg actcgacgac ggtcggcgaa
     1621 gtggtctatt tgaaggacaa aggtggcata cccggggctt cggggcgcta tcttatcgtt
     1681 ggtgaaaccc ttagatacgg gcgctggcgc gcgcagactg cgctgacaac ccgatggctg
     1741 gatggagcat ccgctgactc ttcccgagac aggctttttg cgttgactgc cggatacgac
     1801 ttcacggacc gctggtcgtg tgatgccgtg tggcggcaag ccaaagtatt cggcgagacg
     1861 gcagacagcg tcagggttcg gctcagatat cagcttcctt tctaaacgca ctgctcccga
     1921 gttctctctg tgagggtcgt gtactcaaac gaagcaattg ccttccgagc tccgcctgat
     1981 accgcccggt aactgagaat cccggcaccg tccgttcggc ccatcccccg cgactgaaat
     2041 cgcacatcag ttgtaacagt agcgtcacac agaataaaca taatcgctta cctcgttttt
     2101 acattggggt gtgcggcacg cggcgttatc ggcaggccgg tggtcctcct tctctctaag
     2161 gcacgaaaaa gaatgaatat tcgccaacgg attatcctgc tgatcgctct gaccttcggc
     2221 gcaattgccg ctatcggcgg attcgcgatt ctgcaatccc gcaataacgc tgttgccgtc
     2281 agagcggtga cggaaggtgt ggtaccgagc gcccttgcgg caggcgatct ggttgcgagc
     2341 atcaaggacg tgcaactgac tgcgatctcg atggtttcgg cagcggatga caatgcagca
     2401 gggcgagccg ccgagattct gaaacagttg caggctcaga tcgcgcaatc cgtggatctg
     2461 cagagccagg cggccgatag cgatgcgcag cgcgggctga tcgctcagac tcaggagagc
     2521 ttgaagaact atttcaagtc gattgacgat acgactcgat tcaaactcgc cgggcaaaaa
     2581 gagatggccg aggcgttcct gttcgcgaat gtggcggaat accagactga attgagcggg
     2641 atcgtcgaga ccttgcgcat tgagaagaac cggagcaagg atgcggcgat tctgagcttg
     2701 aacgacaagc tggggcaggt tgttcgaggg atttcgttgg ttacgcttgt tgcggttctt
     2761 gtcctgggct cggttggctc tttcttgtac ctcaggatca cccggcctct caagcagatg
     2821 caggatgcga tcgaaacgat caggacgaat ctcgatttga cgcaccgaat tccggtagca
     2881 ggcaatagcg agattgatcn nnnnnnnnna gatcgatcgg gtcgcggcga gcgtgaactc
     2941 gctgtttgag gagtttcagt ccgttgtcaa gggcgttcag gaggccggca acgcggtttc
     3001 gatgacgtcg gatcagatat tgcagaccgt tgtgcatttg ctgacttcga tcgagataca
     3061 gaacgaagcc acttcggcca tggcggcatc ggttcaggaa atggcggtca gcgttgccca
     3121 tgtctcggat tcgtccgctg tggcgcaggg gattgcgcaa gggtccctgg aaaagtcaaa
     3181 ggacggcgca gaaacgattg aaagaaccgt cacccaaatg gtggtcatgg ccgaggacgt
     3241 gcacctcacg tcgcaggcga tgaaggaatt gggaagacga tcgatcgaaa tcggtgggat
     3301 cactggaacg atcaagaaga ttgctgagct gaccaacttg ctggcgctca acgccgccat
     3361 tgaggccgca cgcgccggag agcatgggct tggattcgcc gtcgttgccg atgaggtgag
     3421 gaaactggcg gagcgaaccg cgcgtgcgac aagagaaatc gaggcggtta ttggtgccgt
     3481 tcagaatgaa acaaagagcg ctgttgatga catgcaccaa atggcgagcc gggtcgtcgc
     3541 gaacgctggc gaagcccgcc atgccggcga gtcgatcgtg cagattcgca acgaatcgaa
     3601 gcatgtcgtc gaggtgtctt ccgaaattgc gacggcactg aaagagcaaa gttcggcgac
     3661 ggaactgttt gccattcaaa tcgaaaaaat cgcctcaatg agcgagaaaa acacggcggt
     3721 aacgggcgag gtgaaagaag tatctgggga cgtcaagcgt ttgtcatcgg agctgtatcg
     3781 attggttgcg cggttccagg tctagaggtg cagggttgaa gcccatcgac cgattgccgc
     3841 tcctgggcgt agcgtcgaag ttggcgagag caatccagaa ccacgactcg ggcgcaaacc
     3901 gatgtggctc tggtgttgca tggcgatgac gatctctcgc gatcgcatgt ttgaaaggaa
     3961 agcatgacgc aaacccgccg tctgcgcagc ctgatgatct ggagtgtggt ctgcgccaac
     4021 atcgcggtgt ttctgctttc cggctactcg ctatatcgaa gccgcctgca atacgaaagt
     4081 cgtgccgaaa cgcttacgca gaatgctgcc agcgctgcag cgaagaacgt cgccaacaat
     4141 atcgacaaga tcgatcttgc cttgcgtacc gtggccgatg aactcgaacg acaaaccgcc
     4201 ggaagaggaa tcgacgataa ggccatggca agtttcctgg ccaaacagga attgcgcttg
     4261 ccagaagtcg aagcatttcg cgtggttaat gccaatggtc ttgtcgtact tggtgatgta
     4321 ttcggccgag agactgatgg aaaatggact gagcacgaca gtttccacta ccaccgagac
     4381 catgccgaca atcaaatgcg ggtcgcgacc ccgcttattg atcacagcac aaacttgtac
     4441 gttctcgatt ttacgcggcg ctatgagagc aaggatggca gtttcgcggg tatcgtggcc
     4501 gctaccgtcg tcgtcgacaa tatcacgctg ctcctgtcgg ggtttggcct cgggacccac
     4561 ggaacaatct atcttcggga tgcctcgctc gcattgattg cccgctttcc gccgattcca
     4621 aacaagtcca tgggccagat cgggaaccgg ctggtttccg aaacggcgcg caagtcgatt
     4681 gcgaccggcg agcggtcggc cacgtatcgc gcggctgccg cggatggctt cgagcgcatt
     4741 tattctttca aacgaatcga acggacgtcg atgcttatcg gggcgggaat ggcccgcgag
     4801 gactatctgg cgggctggac cgaagaagcg tacaagacct tggctaccgc cgtcctgttt
     4861 ctattgctct cgcaactgtt cgccgcagcg tttctgcgat tgctgaacgc aatggggcgc
     4921 gagagcaccc gaaacgagat gtacctgcag tctgccagcg acggcgttca aatctggaat
     4981 gccaaggaaa acaggattgt tgaagtcaat aatcgcctgt gtgaagttct cggctacacg
     5041 cgcgacgaac tgctggcact acagtttggc gagtgggccg tcggctggac ggccgaggtg
     5101 ctgaatggca ggaaattgcc cgaaatgctg gctgcgtcca cgcctgctac catcgagatg
     5161 cggttgcgcc ggaaggatgg cgcgctggtt gacgtcgagg tcagtatcag caatttctat
     5221 ctcgaagacg ttcaatacgt ttacgcctcg tatcgagaca tccgcgagcg caaacaggca
     5281 gagcaaagga tcttgtctct ggcgcatttc gacccactga cccatcttcc aaatcgtcgt
     5341 ttgttgatcg accgactcag tcaggcgttg ctcgccagcg agcgtagtct cgagtacgga
     5401 gccatgatga ttctggatct cgacaatttc aaagccatca acgacacaca gggtcacgat
     5461 gttggcgacc gcctgctggt cgaggtcgcc cgtctcgttt cagcctgcgt gcggaaagag
     5521 gacacggtgg cccgtctcgg gggtgatgaa tatgttgtgt tggtcgagaa tctgggacga
     5581 agtgaagcgt tggcgtcgaa ccaaacggag cagattgcgg agaagattcg caacgcactc
     5641 aaccaggcgt accggaccac gccagatggc cagccctacc aaagcacggc cagcatcggt
     5701 atgacgctgt tcctcggcca atcggattcg accgacgatc ttctgaagca ggccgatgtg
     5761 gcgctctacc aagcgaaggg ggccgggcgc aatgccatac gcttctttga atccagaatg
     5821 caggaactca tcgaagcgcg cctgttgatg gaagcggcct tgcgccaggg tttgcaacaa
     5881 ggcgagcttc atctgttcta tcagccgcag gtcgatcatg aacagcggct tatcggagcc
     5941 gaagcattac tgaggtggct gccgcccgac aaggctcccg tatcaccgat ggagttcatc
     6001 ccgttggcgg aagcgaccgg cctcatcatt ccgatcggcc tttgggtcat gcaaacggct
     6061 tgcgctcagt tgaacgaatg gtcgaagaac gcggcgatgt gccacctgaa gcttgcgatc
     6121 aacgtcagcg ctcgccaatt ccgccagccc gatttcgtcg aacagttgcg cgagagtctt
     6181 gagcgctcag gggccaaccc cagcttgttg aagctcgaat tgacagaaag cgtcgttctg
     6241 gaagaccttg aagatgtcat caatcggatg ctgcagatca aggcactcgg agtgaccttc
     6301 tcgctggacg atttcggaac gggcttttct tccctgtcct acctcaagcg tttgccgctg
     6361 gatcaggtga agatcgatca gtcatttgtc cgcgacgttg cgactgatgc aaacgatgcg
     6421 gctatcgtac gcgcgatcat cgcgatgagt cgctctctcg gcatggaagt gatcgccgag
     6481 ggagtggaaa cgaatgagca gttgacgttc ttgagcgtca gtggttgccc gaattatcaa
     6541 ggatatcttt tcagtcgacc tctgccgctt gcgcagttcg aaacgtttgt cgagcaggct
     6601 ttacgaatct gactgaaaaa gcacgacaaa tccggtgaat caccagtgcg ggactgcgcc
     6661 tgcaagcgcc ttcaactcgg ctctggctcg cttgcactcc gggtacatct gttcaacgat
     6721 tcgccagaat cgcgcaccgt ggttcatttc gcgtagatgg gcgagttcat gcgccacaac
     6781 gtagtcgatt acgtcctcgg aaaagtgaat cagccgccag ttcagccgaa tgcccgtctt
     6841 aagactgcaa ctgccccaac gcgtgcgtgc agacgacagg gccagcggcg gcgcggggac
     6901 gtcgagccgt gcggcaaagt gctgcaggcg ttgggaaaac agcaggcgcg ccttttcgcg
     6961 caaggctttt tcaagttgtg gcgcggcatc cttcggcgag cgcaggcaaa gcgtgagcac
     7021 tggatccgcc agttcgttcc atacggcgcg gttattgccc gtcgcaatac ggatctgtag
     7081 attcttgccg agcatcggca cttgcacgcc gtcgacaatc gccaggggtt cggggcgtag
     7141 ccggcttcgc cattcatcaa gtttctggat tacccagtcg ccatggcgat gaatcagcga
     7201 ctcgacttca ttcagcgacg ctcgaggggg tgcgcccacg cgcaggccgc gctggtcgat
     7261 cgtcagacca atggtgcgtc gtttggcgcg gcgtagcaca taaggaacga ttcggtcacc
     7321 gagcgcaatt cttcgcggcg tttcattttg cgccagcggt ttttcgctgg accggaatag
     7381 atcagcctgc cacggcacag gcggtatccg ggtagcggtg tggcgatagt cgccgcattt
     7441 cgccttcgat ccactgctcg gccagcgtgg tgatttcagc agctgttttg ccggccgggt
     7501 caatggccgg gccgatgctg acggtgatca gtccgggatg tttnnnnnnn nnngccccga
     7561 ctttttgacg aaggcgttcc tcgcccagaa ctcgccggca ttgtgcgcca ccggaacggc
     7621 tggagcgccc gcgctgaccg ccaagtgcgc gccaccgggc ttgaagcggc gtttcgctcc
     7681 gggggcaata cgtgtccctt ccggaaatat cacaacccag tacccggcct tgagtcgatc
     7741 gcggccctga tcgatgacct ggcgtagcgc gtcagtgcca gcactgcggt cgatcgagat
     7801 catcttgacg gcggcgaatg cccagccaag gaacggaatc atgagcagtg actttttcag
     7861 gacaaagacc agcggcggaa agatggcctg caggccgacc gtctcccagg ctgactgatg
     7921 tttggaaaga atgaccgaag gcgttgccgg gatattttct ttgccgatga cctgataacg
     7981 cagcccaagt acgtacctgc acaaggccat gaagccggcg cgccagagag caatgagttg
     8041 aaaacggcat gcgattggca agacgacggc cagagtgacg agcagaccga agggggcggt
     8101 aatcagtatg gcgagcagca gaaacagggt ggaacgaaga aacgcgatca tgatttcaga
     8161 atgtgttcga ccgcttcgga aagatcggca aagaccagcg tgccttccgg caaggtgtcc
     8221 tcccgctggg tgatcaatcc ttttccggtg agcaccagta tcggcctggc gccgagttgc
     8281 gaggccgggt tcaggtcgcg cagggcatca ccgacgacgt aggctttgct caggtcggcg
     8341 ttaaagcacg caccgatgcg ttcgaacatg cccgtcttgg gcttgcggca tttgcatttc
     8401 gaatcggccg tatgcgggca atagaatatg gcattgatgc gcccaccggc gtcctgcacg
     8461 gcatggtgca tcttgtcatg aatggcattg agcgtgtcca tgtcgaacag gccgcgaccg
     8521 at
//
LOCUS       DAORPM010000092         8481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2025_length_8481_cov_12.515547, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000092 DAORPM010000000
VERSION     DAORPM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2025_length_8481_cov_12.515547"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLD53_06030"
     CDS             <1..268
                     /locus_tag="PLD53_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRC60505.1"
                     /translation="ILRSASSLVKKGGRLVYATCSLLDAENEGVVSSFLSAHPEFVTL
                     SAAEVLRRQAIEIDCDHYEGGLRLRLLPHRHGTDGFYSAVMERQ"
     gene            265..1563
                     /locus_tag="PLD53_06035"
     CDS             265..1563
                     /locus_tag="PLD53_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRC60506.1"
                     /translation="MKDRQMFALLQQLWRDLHFSEFYWVALAIVVILVFSRWLSSRLR
                     ERQGSGMPAQNALRTFGAGSLRKIVFPVLALVFVLVLRKVLKAAKLENLSLLDLAVPL
                     LVAWALASVLIYVLRYVFPQGGFLINTERVIKSSIWGWVALEITGFSGPVIELLETVS
                     FGVGSQSINLWMLMHGAVTVCVTLLLALWVASLIESRLMAAGQMDSNVRELLARLAKA
                     ILLVIALLSSLSLVGIDVTTLSVFSGALAVGLGLGLQKIASNYVSGFIILLDRSIRLG
                     NLVAVDERTSGTVTQITTRYTVLRTRTGIEVIIPNEYLVNNVVRNLSFSDTNVRVELS
                     VQVAYSADLVKAMKLLVEAAQSHTRVLDSPAPTVLLSGFADSGVNLELRFWVSDPESG
                     TGSIRSDIGLAVWKAFRENGIEIPFPQREIRILDQSSAPA"
     gene            complement(1960..2124)
                     /locus_tag="PLD53_06040"
     CDS             complement(1960..2124)
                     /locus_tag="PLD53_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60507.1"
                     /translation="MPQFSHIRERFLANARQHLLSPSTTVCECKLEVCLNTLACRTIE
                     TSLPLGNPED"
     gene            complement(2203..3615)
                     /locus_tag="PLD53_06045"
     CDS             complement(2203..3615)
                     /locus_tag="PLD53_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC60508.1"
                     /translation="MTGNSPNNSLLDILGRYKPIPWRGGYSLSRLFFNFYLLVMGSFV
                     VIAFFADFVISTAVKGITDDYTSRFMHGTIVLIEDELFRRPRSEWPKTIKSLDTKFAY
                     RLRIVDRWSLTLPKKQAEKLDSGELAIDSDHDVLYHRLKLTPKILVVGPLSPDANPDR
                     PRALPLELRIHLLTWSLIGLCLAIAVWFWVRPVWRDLEALRQTARDLGEGHFEARAPE
                     ARSRAFELLTDTLNDMAERTQRLIATQRELSSAISHELRTPIARMRFALEMLAESADH
                     PDRQRLMQMMDVDLDELDSLIDSSLTYARFEREQPELHLTPVELAPWLEEEVESMRIL
                     GGALELSVDTSAVPPVQCVELDLKSMPYAITNLLRNALKYAKSKIAVSAEVVEQQICV
                     HVDDDGIGIPPEERKRVFYAFTRLDRSRDRATGGYGLGLAIVRLVLEQHGGTATVDES
                     PLGGARFTLRWPLLQSVTQT"
     gene            complement(3602..4324)
                     /locus_tag="PLD53_06050"
     CDS             complement(3602..4324)
                     /locus_tag="PLD53_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRC60509.1"
                     /translation="MKTRILLVEDDERLAKLTAEYLSKNDFEVSVEIRGDAAESRILE
                     ENPELVILDVMLPGKDGFEVCRSVRAQYKGVILMLTARDEDLDQILGLELGADDYIAK
                     PVQPRLLLARIRALLRRSPGSSANPMKEYAEPYEKTDLVFGQFRISQSTRSTHLGNEA
                     IDLTTAEFDLLWLLARHAGSILSRDDLLQQLRGIGFDGLDRSIDARISRLRRKLGDDP
                     ENPTRIKTVRGKGYLFSKHDWQ"
     gene            4499..5662
                     /locus_tag="PLD53_06055"
     CDS             4499..5662
                     /locus_tag="PLD53_06055"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRC60510.1"
                     /translation="MYAGIVDWPWWAYVLVTLGLTHVTIASVTIYLHRHQAHRALDLH
                     PLPAHFFRLWLWLTTGMGTKEWASIHRKHHAKCETAEDPHSPQIYGINRVLWTGVFLY
                     VKESRNADTLKRYGHGTPDDWIENHLYTPWQKLGVVLMLAVDVAVFGFVYGAMIWAVQ
                     MVWIPFWAAGVINGLGHFWGYRNFNTPDASTNIFPLGLLIGGEELHNNHHTYATSAKL
                     SNKWYEVDLGWGYICILAALGLAKVKNVAPVPVVGKVRATIDFETLQAVIANRYEVLA
                     GYAKSIGQLYREELAKTKNGKQFKGLKRWLAVDASTVPEDSRGRLELLLKQSSALQTS
                     YTMRGELVALWARSNATREQLIKELQNWCHRAETSGVRQLQEFSLRLRSYSLA"
     gene            5674..6162
                     /locus_tag="PLD53_06060"
     CDS             5674..6162
                     /locus_tag="PLD53_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRC60511.1"
                     /translation="MRLSPRSVFGLLALASIGFVAAGLALGEWARLQPCYLCVFQRLL
                     YLLLAFFALCGVLIPRWRRLWCSLVSLTAIGGVITAIEQSWMQYAPEQVIECGYSDPT
                     LLERIVDWFGMQWPAMFMVTGFCTTKDWIFLGLSLANWSVVFFLGLLGVAVWLGFKRK
                     TL"
     gene            6195..7184
                     /locus_tag="PLD53_06065"
     CDS             6195..7184
                     /locus_tag="PLD53_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRC60512.1"
                     /translation="MHSFQSVLTLTFPVFALVLCGYVATRRRMLPIEAIPGLIIFILY
                     FALPAMLFRFGAATPLAQLLDVRLILLYSICALLLVAVTMVLSRNERVGMRDSAFGAL
                     VAAFPNSGFMGIPLILALLGEQAVGVVIVTILVDSFLTSSLCLSMARMQGATGESRQW
                     RTSIQAIFRSLRGAVSNPMPWAIAAGALFGWSGLTMPEPVGKTISLLSDAASPVALFA
                     IGAILARNAVRATHSSPPMDYVPVALAKLFVHPLLVFLASRVAQGMGFAFDTQTLTAL
                     ILVAALPSASNVSMLAERFGVDSDRISRIIMVSTALSFFTFSGLVWVMGIHQA"
     gene            complement(7240..>8481)
                     /locus_tag="PLD53_06070"
     CDS             complement(7240..>8481)
                     /locus_tag="PLD53_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC60513.1"
                     /translation="HVYVHGLIRDAEGQKMSKSKGNVLDPIDLIDGIGVDDLVKKRTT
                     GLMNPNDAAKIEKRTRKEFPDGIQAFGTDALRFTFLSLASPGRDIKFDLHRCEGYRNF
                     CNKLWNATRFVLMNTEGQDCGIDACTPDQCSMKLDFSFADRWIVSKLQRTEAEVTQHF
                     ADYRFDLLARAIYEFIWDEFCDWYLELAKVQMQSGTVEQARATRRTLLRVLETVLRLA
                     HPLIPFITEELWQTVAPLAGRKTHDSIMLAAYPNAQPEKIDETSEAKVKQLKELAYAC
                     RNLRGEMNLSPAQKVPLIACGDRDSLAGFAPYLKALAKLSDMQIVDVLPEDANAPVAV
                     VGETRLMLKIEIDIAAECERLEKEIARLEGEIVKAQAKLGNESFVARAPAAIVEQEKK
                     RLAEFIATVEKLKQQLARLKR"
BASE COUNT         1596 a         2238 c         2595 g         2052 t
ORIGIN      
        1 gattctgcgt tcggcatcca gtttggtgaa gaaggggggg cgtctcgttt acgcaacgtg
       61 cagtttgctc gatgcggaaa acgagggggt tgtgtcgtcg tttctcagcg cgcatcctga
      121 gttcgtcacg ctttctgcgg cggaggtgct gcgcaggcag gccatcgaaa tcgattgcga
      181 tcactatgaa ggaggcttgc gattgcgcct gctaccgcat cgccatggca ccgatggttt
      241 ctattcggcc gtgatggaac ggcaatgaaa gacaggcaga tgttcgcact gctccagcaa
      301 ctctggcgcg atttacattt ctccgaattc tactgggtgg cgctggccat cgtcgtgatt
      361 ctggtgttct cccgctggtt gtcttcgcgg ctgcgtgagc gccaaggcag tggtatgcct
      421 gcgcagaatg cattgcgaac cttcggagct ggaagcctga ggaagattgt ctttccggta
      481 ctcgcgttgg tgttcgtact ggtcttgcgc aaggtgctta aagctgcgaa gttggagaat
      541 cttagtctgc ttgatctggc cgttccgctg cttgttgcat gggcactggc tagtgttttg
      601 atctacgtgc tgcgctatgt tttcccgcag ggcggttttc tcatcaatac cgagcgcgtg
      661 attaaatctt ccatatgggg atgggtagcg cttgagatca ccgggttttc cggtccggta
      721 atcgaattgc tcgaaacggt gagttttggc gtcggcagtc agtccatcaa tctctggatg
      781 ttgatgcatg gcgcagtaac ggtatgcgtt acgctgcttc ttgcactttg ggtcgccagc
      841 ctgatcgaga gccggttgat ggccgccggg cagatggatt cgaacgtgcg cgagttgctg
      901 gccaggttgg ccaaggcgat tctcctggta atcgcactgc tcagcagtct gtctctggtc
      961 ggtatcgatg tgacgacgct ttcggtgttc agcggcgccc tggccgttgg cctcggtctg
     1021 ggccttcaga aaatcgcgag caattacgtg agcggtttca tcatcttgct ggaccgctcg
     1081 atccggttgg gtaatctggt ggctgtcgac gaaaggacat cgggtacggt gacgcagatc
     1141 accacgcgtt acaccgttct gcgcacgcgg actggtatcg aggtgattat acccaatgag
     1201 tatctggtga acaatgttgt gcgcaatctt tcgttctccg acacgaacgt tcgggttgag
     1261 ctgtccgttc aggtggctta cagtgccgat ctggtgaaag caatgaaatt gctggtcgag
     1321 gcggcccaga gtcatacaag ggtgctggac tccccggcgc cgaccgtatt gctttcaggg
     1381 tttgccgaca gtggcgttaa tcttgagcta cgcttctggg tgtctgaccc ggaatctgga
     1441 acgggtagta ttcgttcgga tattggactg gccgtctgga aagccttccg cgagaacggc
     1501 attgagattc cctttccgca gcgtgaaatc cggattcttg accaatcttc ggcacccgcc
     1561 tgattccctg taaggcagca aaaaaaatcc cgcgccggtc tggcacggga ttgaaaggcc
     1621 caccgttgcg atgggtcagg gagggtcaaa cattgccagc gggggacgct gaggcaacaa
     1681 ctgcattcta aggatcggct aatcatgtgt ctgttgcgct tgttcagcct ttgcgtgacg
     1741 gcgtgtaaca cgctgcggaa agcagtcatt cttgcttgtt tcgggtcttc gctttccagc
     1801 aacgccagcc gaaccaggcc agtgagcaac tggcggcaat gagcaaggct ctgatgactt
     1861 ccatgcgtcc atcagcgaga agatcccagg caacaactcc aacgaaaatg ccaactcctt
     1921 cggcgatcgg gaatgttgcg ctgggtcttg tcatagacct tagtcctccg ggtttccgag
     1981 aggcagcgag gtttcgatgg tgcggcaagc cagagtgttc aggcagactt caagcttgca
     2041 ctcgcacact gtcgtgctcg gggagagcaa atgctggcgg gcattggcga ggaaacgttc
     2101 gcgaatgtga ctgaattggg gcattccggg cttcactatc tgattgatca ttgacccggg
     2161 ctatgatagt tggtggaggc gcgtgcgtct tcagtgcttt cgtcaggttt gcgtgacact
     2221 ctgtaacagc ggccagcgga gagtgaaacg tgctccaccg aggggcgatt cgtctactgt
     2281 cgccgtgccg ccgtgttgtt ccagaaccag tcgcacaatg gccagcccca ggccgtagcc
     2341 gccggttgcg cgatcacgtg agcgatcgag tcgggtgaag gcatagaaca cgcgctttct
     2401 ttcttctggt gggatgccga tgccgtcgtc gtcaacatgc acgcaaatct gttgctcgac
     2461 gacttcggcg ctgacggcaa ttttcgactt ggcgtacttg agagcgtttc gtagcaagtt
     2521 ggttatggca tacggcatgc tcttcagatc cagttcaaca cactgaaccg ggggcactgc
     2581 gctagtgtct acggacagtt cgagcgcgcc gccgagaatg cgcatggatt ccacttcctc
     2641 ctcaagccag ggtgccagtt caaccggtgt cagatgcagt tccggctgct cgcgctcgaa
     2701 gcgggcataa gtcaagctcg agtcgatcag actgtcaagt tcgtcaaggt cgacgtccat
     2761 catttgcatc agtcgctggc ggtccgggtg atcagcggat tcggccagca tttcgagggc
     2821 gaagcgcata cgcgcgatcg gggtacgaag ttcgtgcgag atcgcgctgg acaattccct
     2881 ttgggtggca atcagtcgct gggtgcgttc agccatatcg ttgagtgtgt cggtcaggag
     2941 ttcgaaggct ctgctacgcg cctcaggggc tcgtgcttca aaatggcctt ctccaagatc
     3001 tcgtgctgtc tggcgcaatg cttcgaggtc gcgccagacg gggcgcaccc agaaccacac
     3061 ggcgatggcc agacagaggc cgatcaggct ccatgtcaac aggtggatgc gcaattcaag
     3121 cggcaatgcg cgcggtcgat ccgggttcgc gtcgggcgag agcgggccga ccacaagtat
     3181 tttcggcgtg agcttcagcc ggtggtagag aacgtcgtga tcggaatcaa tggccagctc
     3241 gccggagtca agtttttcgg cttgcttctt tggaagcgtg agcgaccagc gatcgacaat
     3301 tcgcaacctg taggcaaact tggtatcaag ggatttgatg gtttttggcc actccgagcg
     3361 cggcctgcga aacagttcgt cctcaatcag gacgatggtg ccgtgcatga agcggctggt
     3421 gtagtcgtcg gtaatgccct tgacggcggt cgagatgacg aaatcggcga aaaaggcgat
     3481 gacgacgaac gaacccatca ccaacaggta gaagttgaag aacagacgcg ataggctgta
     3541 gccgccgcgc cagggtatcg gtttgtatcg cccgagtatg tcaagcagac tattgtttgg
     3601 actattgcca gtcatgtttg ctgaacagat aacctttgcc gcgaaccgtc ttgatgcgtg
     3661 tggggttttc cggatcgtcg ccgagcttgc ggcgcagtcg cgaaatgcga gcgtcaatcg
     3721 agcggtcaag gccgtcgaag ccgatgccgc gcagttgttg aagtagatcg tcgcgcgaca
     3781 ggatgctgcc ggcgtggcga gcgagcaacc acaggaggtc gaattcggcg gtggtcagat
     3841 cgatggcttc attgcccagg tgggtgctgc gtgttgactg actgatgcgg aactggccaa
     3901 agacgagatc agtcttttcg taaggttcgg cgtattcctt catcgggttg gcggaggagc
     3961 ctggcgaacg gcgtaggaga gccctgatgc gcgccagaag caggcgtggc tgcaccggct
     4021 tggcaatata atcatcggct ccgagctcaa gtccaagaat ctgatcgaga tcttcgtcgc
     4081 gggccgtcag cattaggatc acgcccttgt attgcgcacg tacggaccgg cagacctcga
     4141 agccatcctt gccggggagc atcacatcga ggatgaccaa ttcgggattc tcctcgagaa
     4201 tccgggattc cgccgcgtcg ccgcggattt cgacggaaac ctcaaaatcg ttcttgctga
     4261 gatattcggc agtcagtttg gcaaggcgct cgtcgtcctc gaccagcagg attcgtgttt
     4321 tcataatcga atgataacgg gttgaagtcg cagaatatac gtccttgaaa gaaacgacga
     4381 cgcggcaggc gccatttcag ttcgatcacc tcattttgtc gggaaagcga ggcaaatatc
     4441 ggttttgtct tgcctgtcaa atgacttaga atgcttggct gaacttactg gagggaatat
     4501 gtacgctgga attgtcgatt ggccttggtg ggcttatgta cttgtgacgc tgggcctgac
     4561 ccatgtaacg attgcttcag taaccatata cctgcaccgc catcaagcgc atcgcgctct
     4621 ggatttgcat ccgctgcccg ctcatttctt ccgtttgtgg ctgtggctga cgaccggcat
     4681 gggaaccaag gaatgggcgt ccattcaccg caaacaccat gccaagtgcg agacggccga
     4741 agaccctcac agtccacaga tctatggcat caaccgcgtg ttgtggactg gcgtatttct
     4801 ttatgtcaag gaatcgcgca acgccgatac gctcaagcgg tacgggcacg gcacgccgga
     4861 cgactggatc gagaatcatc tctatacccc ctggcagaag ctcggtgtcg tgcttatgct
     4921 ggccgttgat gtggccgtct tcggctttgt ctatggcgcc atgatatggg ccgttcagat
     4981 ggtgtggatt ccattctggg ctgcgggtgt aattaatggc ctggggcatt tttggggcta
     5041 caggaatttt aatacacccg atgccagcac caacatcttc ccgctcgggt tactaattgg
     5101 cggcgaggag ttgcataaca atcaccatac ctatgcgaca tcagccaaac tctcgaataa
     5161 atggtacgag gttgatcttg gctggggcta tatctgtatt cttgcagcgc tgggattggc
     5221 gaaggtgaag aacgtggcgc ccgtcccggt tgtcgggaag gtacgcgcga cgattgactt
     5281 cgaaaccctg caggcggtta ttgccaaccg ctacgaagtc ttggctggtt atgccaagtc
     5341 catagggcaa ctctatcgtg aggaactggc caagacgaag aatggaaaac agttcaaagg
     5401 gttgaagcgc tggttggcgg tagatgccag tactgttcct gaggattccc ggggacgtct
     5461 agaactgctc ctgaagcaga gcagcgcgct gcaaacgagc tataccatgc gcggagagtt
     5521 ggttgccctg tgggcgcgtt ccaacgccac gcgcgagcag ttgatcaagg aattgcagaa
     5581 ctggtgccat cgcgctgaaa cctcgggcgt tcgccaattg caggaattct cgttgcgcct
     5641 gcgcagttat tcgctggcct gaccgaaact gcaatgcgcc tttccccccg cagtgttttt
     5701 ggcttgctgg cgctcgcatc gatcggtttt gtggcggctg gtcttgccct aggcgaatgg
     5761 gccagactgc aaccctgcta cctgtgcgtt ttccagcgtt tgctctacct tctgctggcc
     5821 ttctttgcac tctgtggcgt gctgattcct cgctggcgca ggctgtggtg ttctctggtg
     5881 agtctgacgg ccattggcgg ggtgatcacc gcaatcgagc agagctggat gcagtatgcg
     5941 cccgagcagg tgattgaatg cggctattct gatccgacac tgcttgagcg gattgtcgat
     6001 tggtttggta tgcaatggcc ggcaatgttc atggtcaccg gtttttgcac cacgaaggac
     6061 tggattttcc tcgggctttc attggccaac tggtcggttg tcttcttctt aggcctcttg
     6121 ggcgtcgccg tgtggttggg gttcaagcgc aagacactgt gaaaacgatt cattcagtca
     6181 gcagttgagc gcaagtgcac agcttccagt ctgtcctgac ccttaccttc cccgtcttcg
     6241 cactggtgct gtgcggctat gtggcaacgc gacgccgcat gctgccaatc gaagcgattc
     6301 ccggcctgat catctttatt ctgtattttg cgttgcccgc catgctgttc cgctttggcg
     6361 cggcaacacc gttggcgcag ttgcttgacg ttcgattgat actgctttat tcgatttgtg
     6421 cgctgttgct ggttgcggtg acgatggtgc tcagtcgcaa tgagcgggtc ggtatgaggg
     6481 actcggcttt cggtgcgctg gttgcggctt tccccaatag cggtttcatg ggcattccac
     6541 tgattcttgc gttgctcggc gaacaggccg ttggggtggt gattgtgacc attctggtcg
     6601 atagcttcct gaccagctcg ctctgtttgt cgatggcgcg gatgcaaggc gctacaggtg
     6661 agtcacgcca atggcgcacg tcaatccagg ctatctttcg ctcacttcga ggcgcggtgt
     6721 ccaatccgat gccgtgggcg attgccgccg gggcgctgtt cggctggtcg ggtctgacaa
     6781 tgcccgagcc ggtgggtaaa acgatcagcc tgctgtcgga tgccgcttcg ccggttgccc
     6841 tgtttgcaat tggcgcaatt ctcgcccgca acgcggtgag ggcgacgcac agcagtccgc
     6901 cgatggacta cgtgccggtg gccctggcca agctgttcgt acatccgttg ttggtattcc
     6961 tggcaagtcg cgtagcgcag ggaatgggct ttgccttcga tactcagacg cttacggcgc
     7021 ttatcctcgt tgccgcgctg cccagcgcca gcaacgtttc aatgctcgcc gaacgtttcg
     7081 gtgtcgactc cgatcgcatc tcgcgcatta tcatggtatc cacggcgctg tcatttttta
     7141 ccttttccgg actggtctgg gtaatgggta ttcaccaagc ttgaccgtga tgctgtggga
     7201 aaacgaggtt gccatcctgc gcagtaaaaa ttggcagggt tagcgtttca ggcgcgcaag
     7261 ctgttgcttg agcttctcga ccgtcgcgat gaattcggca agccgcttct tttcttgctc
     7321 gacgatcgcg gccggggcgc gggcgacaaa gctctcgttg cccagcttgg cctgcgcctt
     7381 gacgatctcg ccttcgaggc gcgcgatttc cttttccaga cgttcgcatt cggcagcgat
     7441 gtcgatttcg atcttgagca tcaggcgcgt ttcgccaact acggcgaccg gcgcgttggc
     7501 gtcttccggc aacacgtcga cgatctgcat gtcggatagc ttggccagcg ctttgaggta
     7561 gggcgcgaat ccagccagag aatcccgatc accacaggcg atcagtggaa ctttttgtgc
     7621 cggcgaaagg ttcatctcac cgcgcaggtt gcggcaggca tacgccagct ccttgagctg
     7681 cttcactttc gcttcgctgg tttcgtcgat cttttccggc tgcgcattcg ggtaggcggc
     7741 gagcatgatc gagtcgtgcg tcttccttcc ggccaatgga gcgaccgttt gccagagctc
     7801 ttcggtaatg aaggggatca gcggatgagc cagacgcagc acggtttcca gaacgcgcag
     7861 cagtgtgcgt cgcgtggcgc gggcttgctc gacggtaccg ctctgcatct gtactttggc
     7921 cagttcgaga taccagtcgc agaactcgtc ccagatgaac tcgtagatgg cgcgggcgag
     7981 caggtcgaag cggtagtcgg caaagtgctg cgtgacttcg gcttcggtgc gctgcagctt
     8041 gctgacgatc cagcggtcgg cgaaggagaa gtcgagcttc attgaacact ggtcgggagt
     8101 gcacgcatca atgccgcagt cctggccttc ggtattcatc agcacgaagc gcgtggcatt
     8161 ccacagcttg ttgcagaagt tgcggtagcc ctcgcagcgg tgcaggtcga acttgatgtc
     8221 gcgaccgggg ctggcgagcg agaggaaagt gaagcgcagc gcatcggtgc caaacgcctg
     8281 gatgccgtcc ggaaattcct tgcgcgtgcg cttttcgatc ttcgctgcat cattcgggtt
     8341 catcagaccc gtggtgcgct tcttgaccag gtcgtcgacg ccgatgccgt cgatcaggtc
     8401 gatcggatcg aggacgttgc ccttggattt gctcatcttc tggccttccg cgtcgcggat
     8461 caggccatgc acatagacgt g
//
LOCUS       DAORPM010000093         8464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2030_length_8464_cov_14.698418, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000093 DAORPM010000000
VERSION     DAORPM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2030_length_8464_cov_14.698418"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2093)
                     /locus_tag="PLD53_06075"
     CDS             complement(<1..2093)
                     /locus_tag="PLD53_06075"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC60514.1"
                     /translation="MKKNLIRIALGLLLALVFFGHAAKFYQISLLNTLDAFVYDARLR
                     LTAQGGVDERIVILDIDEKSLAEVGRWPWSRDTMAALVNKLFDSYEITLLGFDVVFAE
                     PDRSSGLASLESMADGVLKNDTAFQSALKALRGELDFDKRFADALSKRPVVLGYYFTN
                     LDQANRNGALPAPVLQPEDFKGRTVDFTSWSGFGGNLPEFQGKAISAGHFNPIVDFDG
                     TSRRVPMLVEHEGKYYESLSLAMVRVLLGNPRIVPGYPEGETNSGPEWLDLPAEQGIL
                     RIPVDEYGASLIPYRGLQGSFTYLSAADALTGRLKQEQLKGKIVLLGTTAPGLMDLRV
                     TPVGSTYPGVEIHANLVAGMLDSSIKQKPSYVLGADVLLLSLCTISLAFLLPILSPLR
                     ATVLASGILLAVACLNFLLWSSGLVMPLAAVLSAILTLYALNMSWGYFVETRSKRQFA
                     ELFGQYVPPELVDEMARDPERYSMDGKNAELTVLFSDVRGFTSISEGLDPKELTQLMN
                     AYLGAMTRVVQKNRGTLDKYIGDAIMAFWGAPVADADHARHAVTTALEMQAELRTLDE
                     PFKQRGWPALHIGIGINSGTMTVGDMGSKVRKSYTVMGDAVNLGSRLEGITKQYGVGI
                     IVSDSTKALVKDVIYRELDRVRVKGKDEPVGIFEPLGVAGQIDKAAQDELKLWNQALR
                     LYRQQDWDQAELQLY"
     gene            complement(2117..2869)
                     /locus_tag="PLD53_06080"
     CDS             complement(2117..2869)
                     /locus_tag="PLD53_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRC60515.1"
                     /translation="MAKLILSMDGLVLKEIPLSKERMSIGRKASNDIQIDNLAISGEH
                     AAVVTILNDSFLEDLNSTNGTLVNGQPAKKHFLKNGDVIELGKYKIKYVAEQVQRADA
                     ADFEKTMILRPSTLRPGSEVTAPAAATVAASATADDTQANKPMHSAPEAVTATTPPPV
                     AAPASGAVQILSGANAGRELMLTKTLTTLGKPGVQVAVIAKRPHGYFITHVEGKQYPV
                     LNGQPIDAQAHQLADHDVIEIAGIKMEFFLKG"
     gene            complement(2882..3712)
                     /locus_tag="PLD53_06085"
     CDS             complement(2882..3712)
                     /locus_tag="PLD53_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRC60516.1"
                     /translation="MVSTLSSALEIVARTDPGMVRRHNEDAVFANPNQGFVILADGMG
                     GYNAGEVASGMATMLLSTELETAFSAKPPHAIDSETGKTFAHRCILDKVALTNSAIHN
                     AAESQPQYAGMGTTLVAALFYDNQVTVAHIGDSRLYRLRGEEFSAITRDHSLLQEQID
                     SGMILAEDAKYSQNKNLVTRALGVDPEVETEIHDYAVQPGDIYLLCSDGLNDMVEDEE
                     IQITVQMLAANLELAATQLIQMANDNGGRDNVSVILVKVLSEFPATRGWWSKLLAWFR
                     "
     gene            complement(3722..6268)
                     /locus_tag="PLD53_06090"
     CDS             complement(3722..6268)
                     /locus_tag="PLD53_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRC60517.1"
                     /translation="MGKTGFWKTDWFLGLAVSCVMLVAANGDLIGSLERKAYDLGVQA
                     SSKTPLDRVAVIAIDDASIANLGRWPWPRDQLARMTDLLAAGKAKVVANTVFFFEPQI
                     DPGYAYVTRLLDIYRQASENVEPSPGLQKIGGVLQEAELALNTDRILTDSIGRAGNVL
                     LPMLFVLGEPRGRPDQVLPEFVAKSRLSQITDGSGDVWQTLSLQVPIEGLGARAAAIG
                     HLNSVVDVDGAIRTEPLVLRHYDDYFPSLSLMIAAASLNLGPADIRVAAGREVRLGSL
                     RIPTDTITQMHTFFYAGHEGKPAFQVDSFYDVLTGKIPASKYQDKIVLIGATASGVGT
                     TQVTPISPAMAPVLTLAHAVSSILQEHFFIAPEWGVWASLGAFVLVAVYLIVLLPRLS
                     AGVGAIATALLLVALVGAHFGLMVGSGLWIQLMASATLLLVGHLMLTTKRFLMTERGK
                     EKSEADSAESNRMLGLAFQGQGQLDMAFDKFRKCPLDDSLMENLYNLALDFERKRQFN
                     KAEAAFRYMADYNPKFRDLEQRLSRAKAMSETVMLGGSGGRTNASTMMLGDGKVEKPM
                     LGRYQIEKELGKGAMGVVYLGRDPKINRIVAIKTMALAQEFEEDELADVKQRFFREAE
                     TAGRLNHQNIVTMYDAGEEHDLAYIAMEFLKGRDLVPYTKPGNLLPLPQVVSILARVA
                     DALAYAHSNNVVHRDVKPANVMYEPASDQVKVTDFGIARITDSSRTKTGMVLGTPSYM
                     SPEQLSGKKIDGRSDLFSLGVTLYQMACGRLPFVGDSMAQLMFRIANEAQPDIRTLNP
                     QVPDGLAEVINRALNKDMNQRYQTGEKMASDLRTCMIQAPAQHPDVDILL"
     gene            complement(6455..7414)
                     /locus_tag="PLD53_06095"
     CDS             complement(6455..7414)
                     /locus_tag="PLD53_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRC60518.1"
                     /translation="MSLFLTEADIRQLLTMPLAIEAVEEAHRELSLGRAVDSPRQRTR
                     LPQTTLHILQGALPGSDAIGYKAYTSNRSGVRFVVHVFSASTGKLRVVLEADLLGMVR
                     TGAASGVATRWLARRDAEVLGVFGAGWQAEGHIEAVAAVRSLRQIKVFARNAERLAAF
                     CTKLSGRLGIDVRAAATAEEVVRDSDIVSTITTAATPLFEASWLSPGTHINAAGSNSL
                     IRREVGEDVIKASNTIVVDSVETALREAGDLLPSLEKGRLRERQLVELGDVISGRHAG
                     RSTPEEITLFESQGMAIQDIALAVRLEALARERGLGIELPYGG"
     gene            complement(7547..8443)
                     /gene="sucD"
                     /locus_tag="PLD53_06100"
     CDS             complement(7547..8443)
                     /gene="sucD"
                     /locus_tag="PLD53_06100"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRC60519.1"
                     /translation="MSILINKNTKIITQGMTGKTGQFHTEKCQEYANGKNCFVAGVNP
                     KKAGESLLGIPIYATVKEAAKQTGATVSVIYVPPPGAAAAIWEAVEAELDLAICITEG
                     IPVRDMLEVRNMMKKREAAGGKKTLLLGPNCPGLITPDEVKIGIMPGHIHKKGRIGVV
                     SRSGTLTYEAVGQLTEIGLGQSTAVGTGGDPINGLKHIDIMRMFNDDPDTDAVIMIGE
                     IGGPDEADAAYWCKENMKKPIVGFIAGVTAPAGKRMGHAGALISGGADTANAKLAIME
                     ECGFTVTRDPSEMGKLLKGLLK"
BASE COUNT         1780 a         2574 c         2276 g         1834 t
ORIGIN      
        1 ttgtaaagct gcaactcggc ctgatcccaa tcttgctgac gatagagacg tagagcctga
       61 ttccacagtt taagctcatc ctgcgccgcc ttgtcgatct gtcctgccac cccaagtggc
      121 tcaaaaatcc cgaccggttc gtccttgccc ttgacccgta cacgatcgag ctcgcggtag
      181 atcacgtcct tgaccagcgc cttggtcgag tcgctgacaa tgattccgac gccgtattgc
      241 ttggttatac cctccagccg tgaccccaga ttcacggcgt cgcccatgac agtataggac
      301 ttgcgcacct tcgatcccat gtcgccaacg gtcatcgtgc cgctgttgat gccaatgcca
      361 atgtgcagtg ccggccagcc gcgctgtttg aaaggctcgt cgagcgtacg caattcagcc
      421 tgcatttcga gcgccgtagt gaccgcatga cgcgcatgat cggcatcggc taccggcgcg
      481 ccccagaaag ccatgatcgc gtcaccgatg tatttgtcga gcgtgccgcg gttcttctgt
      541 acaactcggg tcatcgcgcc caagtatgca ttcatcagtt gcgtcagctc cttgggatca
      601 aggccttccg agatgctcgt aaagccgcgc acgtccgaga aaagcaccgt cagttcagcg
      661 ttttttccgt ccatgctgta gcgttcggga tcgcgcgcca tttcgtcaac cagctcgggc
      721 ggcacgtact ggccaaagag ctcggcaaac tggcgcttgc tgcgggtttc gacgaagtag
      781 ccccaggaca tattcagtgc gtaaagcgta aggatggccg aaagaacggc ggccagaggc
      841 atcacaagcc cggatgacca caggaggaag ttgaggcaag ctactgccag caggattccg
      901 gatgccaaca cggtggcacg caaaggcgat aggatcggca acaggaaagc cagcgaaatg
      961 gtgcacagtg agagcagcag gacgtcggca ccgagcacat acgatggttt ctgcttgatg
     1021 gacgaatcga gcatgccggc aaccagattg gcgtgaatct cgacccccgg ataggtgctt
     1081 ccgacgggtg ttacccgcaa atccatcaga cccggcgctg ttgtaccgag cagcacaatc
     1141 ttgcccttga gttgttcctg tttcagccgc cctgtcaatg cgtcggcagc cgaaagataa
     1201 gtgaagcttc cctgcaggcc acgataaggg atcagactgg cgccgtattc gtcaaccggt
     1261 atacgcaaaa tgccctgttc ggcgggtaaa tccagccact cgggaccact gtttgtttct
     1321 ccctcggggt atcccggtac gattcttgga ttgccgagca acacccgcac catcgccagg
     1381 gaaagggatt cgtagtattt cccttcgtgc tcgacaagca tcggtacccg ccgcgaagtc
     1441 ccgtcgaaat ccacgatggg gttgaagtgc ccggcactga ttgccttgcc ctgaaattcc
     1501 ggcagattgc cgccaaatcc gctccaggaa gtgaagtcga cagttcgtcc cttgaaatct
     1561 tccggctgta gcaccggcgc aggcaaggcg ccattgcggt tggcctggtc gagattcgtg
     1621 aaatagtacc cgagaacgac cggccgcttg ctcaatgcgt cggcgaaccg cttgtcaaaa
     1681 tccagttcgc cgcgcaaggc cttcaatgcc gactggaaag ccgtatcgtt cttgagcaca
     1741 ccgtcagcca tggattccag agacgccagc ccggaactcc tgtccggctc ggcgaagacc
     1801 acatcgaagc cgagaagggt gatctcgtaa ctgtcaaaga gtttgttgac cagtgccgcc
     1861 atcgtgtccc ggctccatgg ccagcgacca acttccgcga gacttttctc gtcgatatcg
     1921 agaatgacga tacgctcatc gacgccaccc tgcgcggtaa ggcgcagccg cgcatcgtaa
     1981 acaaaggcgt cgagcgtatt gagcagcgaa atctgataga acttggccgc atggccaaaa
     2041 aaaaccagcg ccagcaacaa accgagcgcg atccgaatga ggttcttctt caatgcgcct
     2101 ccggcccgga tgaacatcag cccttgagga agaactccat tttgatgccc gcaatttcaa
     2161 tgacgtcgtg gtcagccaat tgatgcgcct gagcgtcaat tggctgacca ttcagtacgg
     2221 gatactgctt gccttcaaca tgggtaatga aatagccgtg aggccttttg gcaattactg
     2281 caacctgaac gccaggcttg ccaagtgtgg tcagtgtctt ggtcagcata agctcacgcc
     2341 cggcattggc accactcagt atctggaccg cgccgctggc gggtgcggcg acgggtggag
     2401 gagtcgtggc cgtgactgct tccggtgccg aatgcatcgg tttgttggcc tgtgtgtcat
     2461 cggctgtagc gctggctgct acggtagccg ccgctggcgc cgtgacttcg gaccccggcc
     2521 gcagggtgct tggccgcaag atcatggttt tttcaaagtc ggcggcatcg gcgcgctgca
     2581 cctgctcggc aacgtacttg atcttgtact tgccaagctc gatgacgtca ccattcttca
     2641 ggaaatgctt cttggccggc tgaccattga ccagcgttcc attggtgcta ttcagatcct
     2701 caaggaagga gtcgttgaga atcgtcacca ccgccgcgtg ctcgccactg atcgccagat
     2761 tgtcgatctg tatatcgttg cttgccttgc ggccaatgct cattcgctcc ttgctcagcg
     2821 gaatttcctt gagaaccaga ccgtccatgc tcagtatcag cttagccatt gtttgccgtc
     2881 cttatctaaa ccatgccaaa agttttgacc accagcctcg ggtagcaggg aattcgctta
     2941 gaaccttgac cagaattaca gagacgttat cacgtccgcc attgtcgttg gccatttgta
     3001 tgagctgcgt tgccgccagt tcaagattgg cggcaagcat ctgcacggtg atctggattt
     3061 cctcgtcttc gaccatgtca ttgaggccat ccgagcacaa cagatagatg tcgccgggtt
     3121 gcaccgcgta gtcatgaatc tcggtttcga cttccggatc cactcccagt gcccgcgtta
     3181 ccaggttctt gttctgggaa tattttgcat cctcggcgag aatcatcccg ctatcgatct
     3241 gctcctgcaa tagcgagtgg tcccgcgtaa tcgcactgaa ctcttctcca cgcagacggt
     3301 aaagccgcga atcgccgatg tgcgcaaccg ttacctgatt gtcgtagaaa agtgccgcca
     3361 ccagcgtggt gcccatcccg gcatactgtg gctgactttc cgccgcattg tggatcgccg
     3421 aattggtcag cgccaccttg tccagaatac agcggtgcgc aaacgtctta ccagtctcac
     3481 tgtcgatggc gtggggtggc ttggcggaaa acgccgtttc cagctcggtg gatagcaaca
     3541 tggtcgccat gccgctcgca acctcacccg cgttgtagcc gcccatgccg tcggccagaa
     3601 tcacgaatcc ctggttcggg ttggcgaata ccgcatcttc attgtgtctt cgcaccatcc
     3661 ccggatcggt tctggcaacg atttcgagtg cgctgcttag cgtgctgacc acctcgtctc
     3721 cctataacag aatatcaaca tcgggatgct gcgctggcgc ctgaatcatg cacgtgcgca
     3781 aatcacttgc catcttttcg cccgtctggt accgctggtt catatctttg ttcaatgcgc
     3841 ggttaatcac ctctgccagg ccgtcaggca cctgtggatt caaggtccgg atatctggct
     3901 gcgcctcgtt ggctattctg aacatcagtt gcgccattga atcgccaaca aaaggcaagc
     3961 ggccgcaagc catctgatag agcgtgacac ccagcgagaa aagatccgag cgcccgtcaa
     4021 tcttcttgcc cgataattgc tcgggcgaca tatagcttgg cgtaccaagc accatgcctg
     4081 tcttcgttct tgacgaatcg gtaatgcgcg ctataccgaa gtcggtgacc ttgacctgat
     4141 cggatgccgg ttcatacatc acgttggcgg gcttcacatc gcggtgaaca acgttattgc
     4201 tgtgcgcata agccagcgcg tcagcgaccc gtgcgaggat gctcaccact tgcggcaacg
     4261 gcaacagatt tccgggtttg gtgtagggca cgagatcccg gcccttcagg aattccatgg
     4321 cgatgtaggc cagatcgtgc tcctcgcccg catcgtacat ggtgacgatg ttctggtggt
     4381 tgagtcggcc ggcagtctct gcttcgcgga aaaaacgttg tttcacgtcg gccagttcgt
     4441 cttcttcgaa ttcctgggcc aaagccatcg tcttgatcgc cacgatgcga ttgatttttg
     4501 gatcgcggcc aagatagaca acgcccatgg cgcctttgcc caactccttt tcgatctggt
     4561 accgcccaag catcggtttt tcgacctttc cgtcaccgag catcatggtg ctggcgttgg
     4621 tacgtccacc cgaacccccg agcatgacag tttcagacat cgctttggct cggctgaggc
     4681 gctgttcgag atcgcggaac ttcgggttgt agtcggccat gtagcggaac gcggcctcag
     4741 ccttgttgaa ctggcgcttt cgctcaaaat ccagcgccag attgtaaagg ttctccatca
     4801 gcgaatcgtc aagagggcac ttgcgaaact tgtcgaaggc catatcaagt tggccttgcc
     4861 cctggaaagc gaggccaagc atgcggttcg attcggccga atccgcttcc gatttctcct
     4921 tgccgcgttc ggtcatcagg aagcgcttgg tggtcaacat cagatgcccg accagcaaca
     4981 gggtcgccga tgccatcaac tgtatccaca ggccgctacc gaccatcagg ccaaaatgcg
     5041 cacccaccag cgccaccaat agcaatgccg tggcaatggc gccaacaccg gcggaaagac
     5101 gtggcaacag gacaatcaaa tacacggcga ccagcacgaa cgccccaagc gaggcccaca
     5161 ccccccattc gggtgctatg aagaaatgct cctgcagaat gcttgaaacg gcgtgcgcca
     5221 gagtcagcac cggcgccatt gcgggcgaaa tcggcgttac ctgagtggta cccacgcccg
     5281 aggccgttgc cccgatcaaa acaattttgt cctgatattt actcgccggg atcttgccgg
     5341 tgagaacatc atagaaggaa tcaacctgga acgccggctt gccctcatgc ccagcgtaaa
     5401 aaaaagtatg catctgcgtt atcgtgtcgg tcggaatacg cagactgccc aggcgaactt
     5461 ctctgccagc cgccacacga atatcggccg gaccaagatt aaggctcgcc gcggcaatca
     5521 tcaatgacag cgaggggaaa taatcgtcgt aatgacgcag caccagcggt tcggtacgga
     5581 tcgcaccatc gacatccacg acggaattca ggtgtccaat ggcagcagcc ctggcgccaa
     5641 gcccttcgat cggcacttgc agtgacaggg tttgccagac atcgccactg ccatcggtga
     5701 tctgcgacag ccggctcttc gcgacaaact ccggcaaaac ctggtccggc ctgcctctcg
     5761 gttctcccaa tacaaacagc atgggtagca ggacattccc ggccctgccg atgctgtcgg
     5821 tcagaatgcg atcggtgttc agcgcaagct cggcctcctg aagcacgccg ccgatcttct
     5881 gcagaccagg cgacggctcg acgttttccg aagcctgccg gtaaatgtcg agcaagcgcg
     5941 tgacataggc gtagccgggg tcgatctgcg gctcgaagaa aaaaaccgtg ttggccacca
     6001 ccttggcctt gccggccgcc aacagatccg tcattctggc cagttgatca cgcggccacg
     6061 gccagcgtcc gaggttggca atcgaagcat cgtcaatggc gatgaccgct actcgatcaa
     6121 gcggtgtctt gcttgatgcc tgcacgccaa ggtcataggc cttgcgttcc agactgccaa
     6181 tcagatcgcc gttggcggca acaagcatga cgcaactcac cgccagaccg agaaaccagt
     6241 cagtcttcca gaatcccgtc ttgcccatgc tcaatcccct cgtggcgcat cgatcaggac
     6301 ggtctcttcg acaccgctcg caatcatgcc gtcaggttat cgcctgcttt ggcgcaatat
     6361 gcgaaggaga aacagcagtc cttggcgcat tgttacagtt ctgcccaatc agagtcaatc
     6421 ggcgcgttcc ggtgccttcc ggtatgttcg gcgattagcc tccatagggc agttcgatgc
     6481 ccaggccacg ctcccgcgcc aaagcttcga gccgcacagc cagcgcaata tcctgaatgg
     6541 ccatgccctg cgattcgaac agcgtgatct cctcgggcgt gctgcgcccg gcatggcgtc
     6601 cgctgatcac atctcccagt tcgacgagtt gccgctcgcg caatcgtcct ttttccagcg
     6661 atggcagtaa atcacccgct tccctgagtg ccgtttccac gctatcgacg acgatggtat
     6721 tgctggcctt gatcacatcc tcgcccactt cccgcctgat tagcgaattg gaacccgccg
     6781 cgttgatatg tgtacccggc gacagccagc ttgcttcaaa caagggcgta gccgccgttg
     6841 ttatggtact gacgatatcg ctgtcgcgca ccacttcttc cgccgtagcg gccgcccgca
     6901 cgtcgatccc gaggcgcccg gacagtttcg tacaaaatgc agcgaggcgt tccgcatttc
     6961 gggcaaaaac cttgatctgc cgtagcgaac gcaccgcggc aactgcctcg atatgccctt
     7021 cagcctgcca gccggcgcca aaaacgccca gcacttcagc atcacgccgg gccaaccagc
     7081 gagtcgccac gcccgaggca gccccggtgc gcaccatgcc aagcaaatcg gcctcaagca
     7141 caacacgcaa ttttcccgtc gaggcgctga atacatggac cacaaaccgc acgcccgaac
     7201 gattgctggt gtaggccttg taaccgatgg cgtcgctccc cggcagggcg ccttgcagga
     7261 tatgcagcgt tgtttgcggt agccgcgtac gctggcgagg tgagtcgacg gcccggccga
     7321 gcgagagctc gcgatgcgct tcttccaccg cttcgatagc caatggcatg gtcaataatt
     7381 ggcggatatc cgcttcggtc aaaaacaaag acatatgctc tccacaagtt aggctagatc
     7441 acgcgtcaga ttatatgcac ccaaagaacc agccgccatt gcaacttctg aaaccacaat
     7501 aaaaaaggca gactgccgtc tgcctttttg ggtgaatcaa tcacacttac ttgagcaatc
     7561 ccttaagcag cttgcccatt tccgacggat cgcgcgtcac ggtgaaacca cactcttcca
     7621 tgatggccag cttggcattg gccgtatcgg caccgcccga aatcagcgca ccagcgtgac
     7681 ccatgcgttt gccggccgga gcggtgaccc cagcgatgaa accaacaatc ggcttcttca
     7741 tgttttcctt gcaccagtag gctgcatcgg cttcatccgg accaccgatt tcaccaatca
     7801 tgatcacggc gtccgtatcc ggatcgtcgt tgaacatgcg catgatgtcg atgtgcttga
     7861 gcccgttgat cggatcacca ccggtaccga ccgcagtcga ctggccgagc ccgatttcgg
     7921 tcagttggcc gaccgcttca taggtcagcg ttcccgaacg cgaaacgaca ccgatgcggc
     7981 ccttcttgtg aatgtggccg ggcatgatgc caatcttgac ctcgtcggga gtgatgagac
     8041 caggacagtt cggtccgagc agcaacgtct tcttgccacc ggcggcttcc cgcttcttca
     8101 tcatgttgcg cacttcgagc atgtcgcgaa ccggaatgcc ctcggtgatg cagatggcca
     8161 gatcaagctc ggcttcgacg gcttcccaga tcgccgcagc ggctccgggt ggaggaacgt
     8221 aaatcaccga gacagttgcg ccggtttgct tggcggcttc cttgaccgta gcgtagatcg
     8281 gaatgccaag aagcgactct ccggcctttt tcggattgac gccagcgaca aagcaattct
     8341 tgccgttggc gtattcctgg cacttttccg tatgaaactg accggtcttt ccggtcattc
     8401 cctgggtgat gattttggtg tttttgttga tcagaatcga catgttggct ccttacttga
     8461 ccgc
//
LOCUS       DAORPM010000094         8345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2084_length_8345_cov_8.021230, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000094 DAORPM010000000
VERSION     DAORPM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2084_length_8345_cov_8.021230"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..754)
                     /locus_tag="PLD53_06105"
     CDS             complement(329..754)
                     /locus_tag="PLD53_06105"
                     /inference="COORDINATES: protein motif:HMM:NF026041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HRC60520.1"
                     /translation="MPVNVLNLPGLRVLDFKETDTDYHVKAQPTVVSKLCPHCGRSNE
                     TVAHAQKTLFIRDLLSHGKSVAIHLDVPRLRCKPCNKGYRSSSEKFKSKVLQEQASNT
                     ATLLTLDRSGEQGGKRLRAHAHDGGLNSCRRFDHTPPLG"
     gene            complement(813..1112)
                     /locus_tag="PLD53_06110"
     CDS             complement(813..1112)
                     /locus_tag="PLD53_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60521.1"
                     /translation="MSNDDAVLLDLNVPTFQKKLFELDAGEVKKVFKTFQKLQALTWD
                     EVFRDHGLKWEQVKNEPGTFTIRLSKSYRAVVEREGALMLFQTLHLDHDGAYGKK"
     gene            complement(1109..1369)
                     /locus_tag="PLD53_06115"
     CDS             complement(1109..1369)
                     /locus_tag="PLD53_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60522.1"
                     /translation="MHNIKTVGKSGQISLGKAMAGMGFIMQELPGGDIVLKRAVVVPM
                     NERLLDPVVHAELEAAREWRRNNPAKATDLTALASKLGISEE"
     gene            complement(1951..2337)
                     /locus_tag="PLD53_06120"
     CDS             complement(1951..2337)
                     /locus_tag="PLD53_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC60523.1"
                     /translation="MAQCLLEGPSKASSRYRSEIAALKRRIEALEKQISRLERMASKK
                     GAPTADNESGTNLRFKPQGVRAQRTRLGLSASEMGALVGVSAQTIYNWEGGTSRPRAE
                     QLAVIAAVRKMGKREVKARLDQMQAD"
     gene            complement(2309..3454)
                     /locus_tag="PLD53_06125"
     CDS             complement(2309..3454)
                     /locus_tag="PLD53_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRC60524.1"
                     /translation="MSFPDKTLIIADRSEAVTEPLHILKSHPHSTDGLVRLGPLMTIP
                     VVLREFGQEPKAILVEIGLKLTQFADPDFKVSYQGVGKLLARCVAITGCEHFGLLVGQ
                     RAAPSALGVAGFMLQSAPDVGTALRDFMQHLGLHDQGGVVALQTLGRISLLGYAIHLP
                     EVEAADQIYDLSIAIGCNIMRSMCGESWNPTEVLLTRPPPQDLAPFSRFFRAPLHFNA
                     VQSALVFPTTWLNHRVRGADALLHQYLEKEAVRLHGKREINFVAEVRALLHRSIVAGQ
                     CTVANISAQLCLHERTLHRRLREHSTTFRRESEEVRSELARRLLTETTMSIKGVSAAL
                     RYSDTSAFNHAFKRWTGDTPTHWRERAKSSQPLATKKQDGAVSVGGS"
     gene            3599..5053
                     /locus_tag="PLD53_06130"
     CDS             3599..5053
                     /locus_tag="PLD53_06130"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HRC60525.1"
                     /translation="MYQSSTYQLWKRFGFVAVVLFLGIGLGTAQAGGSKLTPDEAKQI
                     AVDAYIYGYSLITTDVTRVQMSNVAKVEEIKAPTGTFFNIKRYPPATFRGVSATNADT
                     LYSVAWLDLSEPQVFSHPEIKGRFFTFELVDLWMIVKDSVGTNTSGSKAMTYLFTGPG
                     WKGTVPAGMTHISFPTRYMVVLGRTYAQDSEEDLAKVNALQAQYKVMPLSAYGKPYKF
                     VAPPVNPDPGFSMTDAPQKAIAALGTTGYFNLMTKLMGADAPPSLEDAPMLARMAKIG
                     IVPGQPFDPSKLDPAVQAALKDVPEEAVRRTTAKWESLGKDVNGWRVTTVGGRYGTDY
                     LQRGAWATRGWPSQLPNVSLYPTTYVDSAGEKLSGANKYTLTFPKGQLPPVNPLAFWS
                     ITMYENTPTGLWFYSNPLNKLTVSLRNKLKYNKDGSLTLYFQHESPGKDKEANWLPAP
                     EGLFALTMRLYWAKTSPPSILDGTWQPPGLMKVQ"
     gene            5575..6774
                     /locus_tag="PLD53_06135"
     CDS             5575..6774
                     /locus_tag="PLD53_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRC60526.1"
                     /translation="MEILFTALCAAYDLHHAANAAHNLRMNSGDYKYIWQSNDWPNWR
                     FDLAALAGPMAEVSRAQGLLMGRLADVGMALRDQASLAALTEDVVKTSEIDGEQLNVE
                     SVRSSIARRLGVDIGALAPVDRHVEGMVEMVLDATVNCHAPVSRERLFGWHAALFPTG
                     YSGLSKIKVGAWRDDASGPMQVVSGPIVRQKVHFEAPPADRIETETSRFLDWANSASN
                     EPPLLKAGLGHLWFVTLHPFDDGNGRIARAIGDLLLARADGSTQRFYSLSAQIQRERK
                     AYYNILEGTQKRSMDVTDWLAWFLDTLHRAVGQAQHMLDAVLTKARFWQRWASMPLNE
                     RQVKLLNKLLDGFEGKLSSSKWAAIAKCSPDTALRDINDLLARGVLRKTDAGGRSTSY
                     ELNDLPE"
     gene            complement(6800..7213)
                     /locus_tag="PLD53_06140"
     CDS             complement(6800..7213)
                     /locus_tag="PLD53_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60527.1"
                     /translation="MSRLKFTTAKAIDARKNYSYGSSIREIWFKDDTGKESVIQFSGP
                     FPVRTGNLLGFVYDNTRGHYLAIVNFSTEQYYNFVPEVESMEYNPFPDLGWLFNWLHT
                     STLTSSYGSETVSLNRAVEALICAEISRFNKCKFR"
     gene            complement(7289..7486)
                     /locus_tag="PLD53_06145"
     CDS             complement(7289..7486)
                     /locus_tag="PLD53_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60528.1"
                     /translation="MDATASEGVRLMIGAAVGFGLFLSLDKNGISLFNRKTSVQALGY
                     LFIFSVSWTVLATALMASFGE"
     gene            7706..>8345
                     /gene="istA"
                     /locus_tag="PLD53_06150"
     CDS             7706..>8345
                     /gene="istA"
                     /locus_tag="PLD53_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRC60529.1"
                     /translation="MEMIGKIRRMYFRDKQSLHEITKRTGLSRNTIRKWVREPKEAAP
                     PTYLRVPGPSRLTRFHGVIEQALKADSHRAKQNRRTAKDLLAQIKIEGYVGGYSQLTA
                     FIRAWRGQEGKAPHAFVPLSFELGEAFQFDWSEEGLVIGGIYRRMQVSHLKLCASRAF
                     WLVAYPSQGHEMLFDAHTRSFTALGGIPRRGIYDNMKTAVDKVNKGKGRVVNA"
BASE COUNT         1757 a         2409 c         2417 g         1762 t
ORIGIN      
        1 gagtgatagt gcaacgccta ctcccggaca gcagttcggc cttatgtgaa gctttccata
       61 atgcagaggg tttttcgctt ggcggacgcg gcctgccgag ttcagtgata ggcatggtgc
      121 cacaccgcga ccggtaggct aaaaatcctg ttggactgaa ccgcctgcgc aggcggtggt
      181 gctatgggga ttgaatcaaa gcggcctcat tgaatcgaga tgcgggcaga ggcgagaagc
      241 cggatgccac aagccaggtc gttgtactac ttctccacgg cactgaagtt gcactgcaga
      301 attgatcggg tgcacagggc gattgatttc atcctagggg aggggtgtgg tcgaatcggc
      361 ggcaggaatt gaggcctccg tcgtgggcgt gagcccgaag acgctttccg ccttgttctc
      421 cagatctgtc caaggtgagg agcgtcgcgg tattggaggc ttgctcctga agaactttgc
      481 tcttgaattt ttctgaactg cttcgatacc ccttgttgca gggcttgcag cgcaggcgcg
      541 gcacatcgag gtggatcgct acactcttgc catgcgaaag caggtcacgg atgaagagtg
      601 tcttctgggc gtgagcgacg gtttcgttgg atcggccaca gtgcgggcag agcttggaaa
      661 cgacggtcgg ctgtgccttt acatggtagt cggtatcggt ttccttgaag tccagcactc
      721 gcaagccagg gagattgagg acattaacgg gcataagcag ggttcaggtg tggtatcccg
      781 ctaagccaat tgagcaaaac ggagtgcctt ttctattttt tcccgtaggc accgtcgtga
      841 tcaaggtgga gagtttgaaa cagcatcaac gcgccctctc gttcgacaac ggcgcgatac
      901 gattttgata gtcgaatcgt gaatgtgcct ggttcgtttt tgacttgttc ccacttcaac
      961 ccgtggtcac ggaagacctc gtcccaggtt agcgcttgaa gtttctgaaa cgtcttgaat
     1021 accttcttga cctctccggc atcaagctca aacagcttct tctgaaacgt aggcacgttc
     1081 aggtctaaca gcaccgcatc gtcgttgctc actcttccga aattccgagt ttggaagcaa
     1141 gcgcagtcag gtcagttgct tttgccggat tgtttcgccg ccactcgcgg gcagcctcca
     1201 gttccgcatg gacaaccggg tcgagcagtc tttcgttcat aggaacaaca acagcccgtt
     1261 tgagcacgat gtcgcctccg ggcagttctt gcataatgaa gcccatcccg gccattgcct
     1321 tgccaagcga aatttgcccg ctttttccaa ccgttttgat attgtgcatt acgctctcct
     1381 ccgtgccgca tgatcgcatg tctgcatgat acatccaatt tggtgctatg ggaatttgat
     1441 tcgagcgagg cgacgttgtt tacaggagca ggtaccggca atagcgcata cagggaagca
     1501 tcgtcaaccc gacacgtagc acatgtgcag tgcagcactt tctctcaagg agtcttgttt
     1561 aagcactgaa ctggctatgt ctcagtacga cccaatccgg ccggtcgcct ttctgaacgt
     1621 tcaatgacag gtaactgaat acagcagccg ttacagccca gtgctcattg gcaaatgctc
     1681 ggccaaaggc gacaacggcc tttcgattga ccgcgtccgc tgtagtcgca agaccggtcg
     1741 ttggctcgcc ggtcgctcct gaacgatcgc tttcctcagt tcagttccat ttccctatcc
     1801 gtccaatgtt gcccaaaccc ttccgcgacg tcattctcgt tctagacgag ttccgcgatg
     1861 gtccagtcgg cgaatatgtc cttcaggatt tctggggtgt ggcggttctc gtacggtgga
     1921 ggggcggccg atgcggtgcg ttctggtcca tcaatccgct tgcatctggt caagccgtgc
     1981 cttgacctcg cgcttgccca ttttccggac cgccgcgatg acagcgagct gctcggccct
     2041 tgggcgcgac gtgccgcctt cccagttgta gatggtttgg gcggagacgc ccaccagcgc
     2101 gcccatctcc gacgcggata gtccgagccg cgtccgctgt gcacgcacac cctgcggctt
     2161 gaatctcaga ttcgttccgg attcgttgtc ggcggtaggc gcgcctttct tcgacgccat
     2221 tctttcaagg cgggatatct gcttttccag ggcctcgata cgccgcttta aggcggcgat
     2281 ctcggagcgg tagcggctcg acgccttgct aggaccctcc aacagacact gcgccatcct
     2341 gcttcttagt ggcgagcggt tgtgacgatt tcgcacgttc ccgccagtgt gtaggcgtat
     2401 cccccgtcca ccgtttgaac gcatggttga aggcactcgt gtcgctataa cgcagggcgg
     2461 cagatacgcc tttgatcgac atggtggttt cggtgagtag ccgccgtgca agttcagaac
     2521 ggacttcctc agattcgcga cgaaaagtcg tgctgtgctc gcggagcctg cgatgcaggg
     2581 tgcgctcatg cagacagagc tgggcagaga tatttgcgac ggtgcattgc cctgccacta
     2641 ttgacctgtg gagcagcgct cgcacttcgg cgacgaagtt tatttcgcgc tttccgtgca
     2701 gtctgactgc ttccttctcg aggtattgat gtagcagcgc atccgcaccc cgcacgcggt
     2761 ggttcaacca tgtggtaggg aaaaccagtg cactctggac ggcattgaag tgcaggggtg
     2821 cgcggaaaaa ccgactgaaa ggtgcgaggt cctgtggcgg cggccgcgtc aaaagcacct
     2881 cagtagggtt ccagctttca ccgcacatgc tgcgcatgat gttgcatcca atggctattg
     2941 agaggtcata aatctgatcg gcggcttcca cttcaggcag atggatggcg tagccaagga
     3001 gggagatgcg ccccaaggtc tgcagcgcta ctacgccgcc ctgatcatga agacccagat
     3061 gttgcatgaa gtcacgcagc gccgtaccca catcgggggc gctctgcagc atgaaacctg
     3121 caacaccgag agcggaaggt gcagcgcgct gtcccaccag taaaccgaag tgttcacatc
     3181 ccgtgattgc tacgcacctt gccagcaatt tacccacgcc ctggtaggaa accttaaagt
     3241 cgggatccgc gaattgagtc aatttcaagc cgatctcgac caggatggct ttgggctcct
     3301 ggccgaactc gcgcaggacc accggtatcg tcatcaatgg accgagacgg acaagcccgt
     3361 cggttgaatg ggggtggctt ttcaaaatat gcagtggttc cgttacggct tctgacctgt
     3421 cagcaattat caaagttttg tcaggaaacg acaatatatt gaagcagaat gcgcttactc
     3481 ttgttgctca ttatttttaa catggaacta ggggttggac ggttctgtgg gctgcccctg
     3541 ctacagcgcc gaaatggggc tggcgctgac tgacaacgat tgaatggagt aacaagatat
     3601 gtatcaatca agcacttacc aactgtggaa gcgattcgga tttgtggcag tggtcctgtt
     3661 cctgggcatt ggccttggca cggcgcaggc cggcggatcc aaactgaccc cggatgaagc
     3721 caagcagatt gcggtggatg cctacatcta cggttattcg ctcatcacca cggacgtcac
     3781 ccgtgtacag atgagcaatg tcgctaaggt ggaggagatc aaggcgccga cgggtacctt
     3841 cttcaacatc aagcgctacc cgccggccac cttccgtggc gtctcggcga cgaatgccga
     3901 cacactgtac tcggtggcgt ggctcgacct gtccgagccg caggtgttca gccatcccga
     3961 gatcaagggt cgtttcttca ccttcgagct ggtcgacctg tggatgatcg tcaaagatag
     4021 cgtcggcacc aataccagcg gctcgaaggc gatgacctac ctgttcaccg gcccgggctg
     4081 gaagggaacc gtaccggcgg gcatgacgca tatcagcttc ccgacgcgtt acatggtcgt
     4141 cctgggccgc acctacgcgc aggacagcga agaagacctc gcaaaggtga acgccctgca
     4201 ggcccagtac aaggtcatgc ccctgtcggc ctatggcaag ccttacaaat tcgtggcgcc
     4261 cccggtcaat ccggacccgg gcttcagcat gaccgatgcg ccacagaagg cgattgctgc
     4321 cctgggcacg accggctact tcaatctgat gacgaagctg atgggagctg acgcgccgcc
     4381 ttcgctggaa gatgccccca tgctggcgcg catggccaag atcggcatcg tgccaggaca
     4441 gcccttcgac ccgagcaagc tcgatccggc ggtgcaggcg gcactcaagg atgtgcccga
     4501 ggaggcagtc cgtcggacga ccgccaagtg ggaatcgctg ggcaaggacg tgaacggctg
     4561 gcgtgtcacg acggtcggtg gccgctacgg cacggactat ctccagcgcg gcgcctgggc
     4621 cacccggggc tggccttcgc aactaccaaa cgtctccttg tatccgacca cctacgtcga
     4681 cagcgcaggg gagaaactct cgggtgccaa caagtacacg ctgaccttcc cgaagggtca
     4741 gttgccgccg gtcaatccct tggccttctg gtcgatcacg atgtacgaga acacgccaac
     4801 gggcctgtgg ttctactcca acccgttgaa caagctcacc gtgagcctcc gcaacaagct
     4861 gaagtacaac aaggacggat cgctgacgct gtacttccag cacgaatcgc cgggcaagga
     4921 caaggaggcc aactggcttc ccgcacctga agggctcttc gcgctcacga tgcgactgta
     4981 ttgggccaag acttcgccgc cgtcgatcct ggatgggact tggcagccgc cgggcctgat
     5041 gaaggttcag taacagcacg cgaaggcgtg ccagagcggc gcacactcag atcaatggat
     5101 cgacgtgact gactggcaca cggcagctca aaagcccaca gcggacgtgc ttgccttgtt
     5161 ggacgtaaga ccgcttcggc cgaacagccc gcgtacggta tccggcaggt tacggagcgg
     5221 accagccgtt cgaatggcgg ctgtgtagaa gtggagaccg gcaggtcatg gccggactcg
     5281 gacctagctc aacgctcgat tttacatgat gtatcaattt tgcactgcac aacgcgtaac
     5341 caaaatctta ctcgtccacc ggaaaatccg gatgccccat taaatgatcc cggttttgag
     5401 ttttcaacag attaacttaa cggctggggt tttccgttcc actgcacctc aaaccccaac
     5461 ctctgcccgc aggcgctctc gcatcatcca gcctcgttac gtgcagggca gaagtcaaac
     5521 aaacccaaac cagtcgacac ccattccggc gtgaggcagc ccgctgtgcg gcgaatggag
     5581 atcctattca ccgcattatg tgcggcgtat gacttgcatc atgcggcgaa tgcagcccat
     5641 aatctccgca tgaacagcgg cgattacaaa tacatctggc aatccaacga ctggccgaac
     5701 tggcgcttcg atctggcggc actcgctggc cctatggctg aggtcagtcg tgcccagggg
     5761 cttctgatgg gccgtctggc cgacgtaggc atggcgctgc gcgatcaggc cagcctggcc
     5821 gcgctcaccg aggacgtggt caagaccagc gagatcgatg gcgagcagct caatgtcgaa
     5881 tccgtacgct cgtccatcgc ccgtaggctc ggcgtggaca tcggcgcctt ggccccggtg
     5941 gatcggcacg tcgaaggcat ggtcgagatg gtgctcgatg ccaccgtcaa ctgccatgct
     6001 ccggtgtcgc gggagcggct gttcggctgg catgccgcgc tgtttcccac cggctactcc
     6061 ggactctcca agatcaaggt cggcgcctgg cgcgacgatg ccagcggtcc gatgcaggtg
     6121 gtgtctggcc ccatcgtccg ccagaaagtg catttcgaag cgccgccggc cgatcgtata
     6181 gagaccgaaa ccagccgatt tctggactgg gccaatagcg catcaaacga accgccgctg
     6241 ctcaaggccg gccttggcca tctttggttc gtcaccttgc acccgttcga cgacggcaac
     6301 ggtcgtatcg cccgggccat cggcgacctg ctgctggccc gcgccgacgg cagcacgcaa
     6361 cgcttctaca gtttgtccgc gcagatccag cgggagcgca aggcctacta caacatcctg
     6421 gaggggaccc aaaagcggtc gatggacgtc accgattggc tcgcatggtt cctcgacacc
     6481 ctccatcgcg ccgtcggtca ggcgcagcac atgctcgacg ccgtgctgac gaaggcccgc
     6541 ttttggcagc gctgggcgag catgccgtta aacgagcgac aggtgaagtt gctcaacaag
     6601 ctgctcgatg gctttgaagg caagctctcc agcagcaagt gggcggccat tgccaagtgt
     6661 tcgccggaca cggccctgcg cgatatcaac gatctgcttg cgcggggagt gctgcgaaaa
     6721 acggatgcag gtgggcgcag caccagctac gaactgaacg atctgccgga gtagccgcgc
     6781 ctggaattga cttggctcat tacctgaatt tgcacttatt gaatctcgag atctcggcac
     6841 agatcagcgc ttccacggct cggttcagcg aaaccgtttc tgaaccatag gaactggtca
     6901 gcgtacttgt atgcaaccaa ttgaagagcc acccgagatc ggggaacggg ttgtactcca
     6961 tgctctccac ttcgggcacg aagttgtaat actgttccgt cgaaaaattg acaatcgcga
     7021 ggtaatgacc acgggtgttg tcatagacga acccgagaag gttgccggtt cgcaccggga
     7081 acggtcccga gaactgaatg acgctctctt tcccggtatc gtccttgaac cagatttcgc
     7141 ggatcgaact accgtaggaa tagttctttc gggcatcgat ggccttggct gtcgtgaatt
     7201 tgagtcgtga catgtctttt gctcacagga ttcgttcgtt gatgtgcccg aggcaccttc
     7261 catccttctc gtgattcggc agggccgatc actccccgaa ggacgccatg agtgcggtcg
     7321 ccaggacagt ccagctcacg ctgaagatga acaggtaacc cagcgcctgg acactcgttt
     7381 tacgattgaa gaggctgatc ccgttcttat ccagggagag gaacagcccg aacccgacgg
     7441 cagcgccgat catcagccga acgccttcac tagcggttgc gtccatggcg gcctccgcgg
     7501 cgacgggatg aacgggaggc gatacggcgt accgtttcat tcctgaagtg gtagaccaga
     7561 tcgatcaact catcctcggt gaaatgcggg ctgttgacgc tgaccgaata gtgacccagc
     7621 aataggcgaa atgctgacgt aaaactgacc caggtgttcc actagttccg cctaattttt
     7681 aggcgggaga taaaaggtga tcaccatgga aatgataggc aagattcgac ggatgtattt
     7741 ccgcgacaag cagtcgctgc acgaaatcac aaagcgcacg gggttgtcgc gcaacacgat
     7801 ccgcaaatgg gtgagagagc ccaaggaagc tgccccaccg acttacctca gggttccggg
     7861 accaagccga ctcacgcggt tccacggagt cattgagcag gcgctcaaag ccgattcgca
     7921 ccgcgccaaa cagaaccggc gcactgccaa ggatttattg gcgcaaatca agatcgaagg
     7981 ttacgtaggc ggctacagcc aactcactgc cttcatccgc gcctggcgcg gtcaagaagg
     8041 caaagccccg cacgcctttg ttcccctgag tttcgagttg ggcgaagcat tccagtttga
     8101 ctggagcgaa gaaggactcg tgattggcgg catctaccga cgcatgcaag tttctcatct
     8161 caagctgtgc gctagccgcg cgttctggtt ggtagcctac ccgagccaag gccacgaaat
     8221 gctgttcgat gcccacacgc gatcctttac cgcgctgggc ggcattcccc gtcgcggcat
     8281 ctatgacaat atgaagacgg cagtggacaa ggtcaacaag ggtaagggac gtgtggtcaa
     8341 tgcgc
//
LOCUS       DAORPM010000095         8288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2104_length_8288_cov_17.622130, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000095 DAORPM010000000
VERSION     DAORPM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_8288_cov_17.622130"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1948)
                     /locus_tag="PLD53_06155"
     CDS             complement(38..1948)
                     /locus_tag="PLD53_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC60530.1"
                     /translation="MVKLLQQLVRWIFMRIENVFNLAFGEKLNPFYHLGTLSFWQFWL
                     LVGSGLYLYIFADTGIDQAYLSVERITHDQWWAGGILRSIHRYATDGMILTMLLHMLR
                     HFAYDRYRGFRSFSWLTGVALLWMVYIAGINGFMLVWDKLAQFVVIATAEWFDALPMF
                     GGTLIRNFIYLENVGSRLFTLLAFMHIGVPLIVVVIMWVHVQRVPRAHINPPRPIAIA
                     ITLMFVALALVKPILSQGGEADMSVVPTNIEFDWFQLPVLALVYVFDPLYLWLGVMGF
                     TGLLALTPWLPPKRRGAAQPTTRITFRPDHKSVTARFGETLLDAGLRQDINLPYECRN
                     GGCGVCKCTVLQGQVDPGLYQPSALSAEELSQGKVLLCSASAINDVEIEYAASTVEKA
                     FREYAARVVKLERLTYDVMRILLKLPDDQQIGFKAGQYINIILDDGERRAFSFANPPH
                     EPEFVELQIRLVEGGRFTTHVFEKMKEGDEIRFEGPLGDFALRESERPIVFVAGATGF
                     APVKSMVEDAFKRGLTRPIFLYWGVKQLRDLYLPELPKRWASEHANFHFIPVLSEPAQ
                     EDDWHGRTGLVHEAILADFPELKGHEIYACGSVKMVESIFPFLKKQGAEDGACFSDAF
                     SVSARSMAFQPK"
     gene            complement(2022..2522)
                     /locus_tag="PLD53_06160"
     CDS             complement(2022..2522)
                     /locus_tag="PLD53_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60531.1"
                     /translation="MLKPVAIVGQIVLYGAFAVFIGYFATYPLYRQVPDDSALIKLSM
                     SHLGGRECRKRTPEELEKMPPNMRAPLDCPRHRSDISLEVDLDGQPFFRSVMHPTGLS
                     KDGVSTIYKRFEVKAGSYQLTVRMNDNLINPGFNHVKDEQITLKPAQVLVIDFNPDKG
                     GLFFRQ"
     gene            complement(2515..4086)
                     /locus_tag="PLD53_06165"
     CDS             complement(2515..4086)
                     /locus_tag="PLD53_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HRC60532.1"
                     /translation="MSFHASLRNTAQTLFQRAEEFFDVYFGGNNNPLRHLGAMGLYFL
                     WIIVGTGLYLYTVLDTSIDGVYNSIGYLSSDQWYLGGILRSLHRYASDAFILVMVLHL
                     LREWAFGRYYGFRFYSWITGVPILWLSYVSGISGYWIVWDQLAQFSATASTELLDWLP
                     IFSEPAARNFLSPGSLNDRFFTMLVFIHLGIPLMLILGLWAHVHRISRVDYLPSRQVM
                     LSTLGALLLLALIKPALSDAPANLAVVPAQLRLDWFLLFIHPLTDATSPAIVWVMLFA
                     ATILLFVLPFLPHPKVEPIAVVDATNCNGCSRCLADCPYAAITMGPHPDRPGYKLAIV
                     ASELCAGCGICAGSCPSSTPFRRQERLVTGIDMPQQSVNDLRRQLEDHLARLVGHTRI
                     VVFGCKCGANADALAAPDTAVISLLCTGELSPAFVEYALRGGADGVLVASCRDGGCEY
                     RLGDRWTAERLSGRREPHLRSKVPITRVQQVFASVNDNKVLAEALCEFRDRIEIQKDF
                     DDPLLPYVRRNTHHA"
     gene            complement(4222..5100)
                     /locus_tag="PLD53_06170"
     CDS             complement(4222..5100)
                     /locus_tag="PLD53_06170"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRC60533.1"
                     /translation="MTFKVCVALFAAVSALAAAPLVAADSGSAPENPKLTARPQGLNT
                     GLAITTLDEKAAFGLSQSVIGKPVGDFVLLDRSGKPVELSRYRGKPLLVSFIYTACFQ
                     VCPTTTRNLQKAVRNTVSVLGADRFNVISIGFNQPFDSPQALKDFSAQYGINLPNWEF
                     LSPSPAILDELTRNFGFSYVATPAGFDHLNQVTMVDAQGKIIRQVYGEKFTAEDLAEP
                     LKVMITGSPIPPQVGTLQEIMARVRIICSVYDPVTGRYRTNYSLYFQIAGFLTFVGFM
                     IYLSFNFWKNRRRSEK"
     gene            complement(5117..6031)
                     /gene="cyoE"
                     /locus_tag="PLD53_06175"
     CDS             complement(5117..6031)
                     /gene="cyoE"
                     /locus_tag="PLD53_06175"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRC60534.1"
                     /translation="MSTTLLTPMQSTPPANRAAGISVRTILGVFKLRIGVVITFTALA
                     GLAVSPGAGLSWWQIVVLALSVLISSASAGAFNQYYERDLDPLMARTSKRPFATGEIG
                     SGPVWLLIIGALSLLSVGAAWIALNAWSALYVFLGAFFYAIVYTVWLKRHTWLNIVFG
                     GLAGSWAVLAGAAAADPHIGAVPLALAFVLFLWTPPHFWSLAIAFRADYAAIGVPMLP
                     VVVGDNRAAKVILVSTLALVAASLLPLAFGLGAIYFAGAVTGGLLFIQKAWLLARDTN
                     RKTAMTCFHASLIQLTLVLLGAIIDAQF"
     gene            complement(6131..7831)
                     /locus_tag="PLD53_06180"
     CDS             complement(6131..7831)
                     /locus_tag="PLD53_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRC60535.1"
                     /translation="MATTYRTCPSSGLQFENNAEKLMRWHAVVGVLWLLVGGITALFV
                     ILTRWPAIKLLPADQFYLILTAHGIDMLILWILHFEMAVLFFASSTLLRCRLATPKLA
                     WLSFWLMVIGGVVCNVAIFQGESSVMFTSYVPMQAAPHFYLGIILVAVGALIGTGIFF
                     ATLVVAKKDKTYQGSLPLVTFGAMTAAVIATFTILAGAGILIPTFLWSVGLIKEIDAQ
                     LYRMVWWGLGHSSQQINVAAHVSIWYLTAAVLFNAKPLSEKVSRFAFLLYILFLQLAS
                     AHHLLSDPGMSAEWKVLNTSYFMYFAVMASMIHGFTVPGAIEAAQRRKGYTNGLFEWL
                     RKAPWGNPAFSGMFISLVGFGFLGGISGVVLGTEQINMLMHNTFYVPGHFHTTVVVGT
                     TLAFMSITYILVPTLFRREMICPKLCQIQPYLYGISMSVLGLVMMGAGTLGVSRRHWD
                     MAFSGAPFQYEWPGAAYLMMGLTGIFGVIAVLGGGLWILLVVGSLLFGKKLDGGKVSD
                     KPTPIPSNTEAASAVLHTDKGDHVAVPGTVVLALTLLVTFVLYYFVNWKYLSEVWLLS
                     "
     gene            complement(7845..>8288)
                     /locus_tag="PLD53_06185"
     CDS             complement(7845..>8288)
                     /locus_tag="PLD53_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60536.1"
                     /translation="FVMMVYWHIYGKQNLSNEAYKTTPEMFAAKAEKFIAENTVRSET
                     DLNGQQIPVVKVPAGGDGFLIARLWNWYPILELEKGQTYKIHLSSMDYNHGFSLQPVN
                     INIQVIPGYEHVVKMTPTKEGTYAIVCNEYCGIGHHSMLGRIYVK"
BASE COUNT         1993 a         2286 c         2474 g         1535 t
ORIGIN      
        1 gttgacagca gtacggccag atcagaagag tgcgcgttta cttcggctgg aaagccatcg
       61 agcgcgccga taccgagaac gcgtcggaga aacacgctcc atcctccgca ccctgcttct
      121 tgaggaaagg aaagatggat tcgaccatct ttaccgaacc gcaggcatag atttcgtgcc
      181 ccttgagctc gggaaagtcg gcgaggatgg cttcatggac gaggccggtt cggccgtgcc
      241 agtcgtcttc ctgcgccggt tctgaaagca ccgggatgaa gtggaagttg gcgtgttcgc
      301 tggcccagcg tttgggcagt tccggcaggt agaggtcgcg caactgcttg acgccccagt
      361 agaggaagat cggccgcgtc aaaccccgct tgaaggcatc ctcgaccatg ctttttactg
      421 gcgcaaatcc cgttgcgccg gccacgaaaa caatcggccg ctccgactcg cgcaatgcga
      481 aatcgccgag cggtccttca aaacggattt catcgccctc cttcattttc tcgaatacgt
      541 gggtcgtgaa tcggccacct tcaacgaggc gaatctgaag ctcgacgaat tcgggttcat
      601 gcggcggatt ggcgaaggaa aaggcacgcc gctcaccgtc gtcgagaatg atgttgatgt
      661 actgcccggc cttgaagccg atctgttggt cgtcaggcag cttgaggaga atgcgcatga
      721 cgtcgtaggt gagcctctcc agcttcacga cgcgcgcggc gtattcgcgg aaagcttttt
      781 ccactgtgct tgcggcatac tcgatttcaa catcattgat cgcgctggcg gaacaaagaa
      841 gtactttccc ttgcgacagc tcttctgcgg agagcgcact gggctggtac aggccgggat
      901 caacctgacc ttgcaatacg gtgcacttgc acacgccgca gccgccgttc cggcactcgt
      961 agggcagatt gatgtcctgg cgcaatccgg cgtcgagcag tgtttcgccg aatcttgcgg
     1021 tcactgactt atgatcggga cggaaggtaa tacgggttgt aggctgcgcc gctccgcgcc
     1081 gcttgggtgg cagccacggt gtgagcgcga gcaggccggt gaaacccatg acaccgagcc
     1141 acagatacag cggatcgaac acatagacca gggccagaac gggtagctgg aaccaatcaa
     1201 attcaatatt ggtgggcacg accgacatat cggcttcgcc accctggctc aggatgggtt
     1261 tgaccagcgc gagcgcaaca aacatcaagg taatggcgat cgcaatcggc cgtggcgggt
     1321 tgatgtgggc gcgaggcaca cgctgcacat ggacccacat gatgacgacc acaatcagcg
     1381 gcacgccgat gtgcatgaac gccagcaggg taaataacct gctgcccacg ttttcaaggt
     1441 aaatgaagtt gcggatcagg gtgccgccga acatcggcag ggcatcaaac cattcggcgg
     1501 tggcaatgac gacaaactgc gcaagcttat cccagacgag catgaagcca ttgatgccgg
     1561 caatgtagac catccacagc agggcgacgc cggtcagcca cgaaaaagac cggaagccgc
     1621 gatagcggtc ataggcgaag tggcgaagca tgtgcagcag catggtgaga atcatgccgt
     1681 cagtcgcata acggtggatg ctgcgcaaga ttccgccggc ccaccactgg tcgtgtgtga
     1741 tgcgttcaac cgacaaatag gcctgatcaa tcccggtatc ggcaaagata tacaggtaga
     1801 gtccggagcc gaccaataac cagaattgcc agaagctgag tgtgccaagg tggtagaagg
     1861 ggttcagttt ttcgccaaac gcaagattga aaacgttttc gatgcgcatg aagatccagc
     1921 gcacgagttg ttgaagaagt ttgaccattg tcgatgtccg ctcaggagac tgcagctggt
     1981 agggaaggga tgcgccgaat agtgaacaag tccgccatga ttcactgccg gaaaaagagg
     2041 ccgcccttgt cggggttgaa gtcaatcacc agaacctgcg cgggcttcag cgtgatttgc
     2101 tcgtccttca cgtgattgaa accgggattg atcagattgt cattcatccg caccgtcaac
     2161 tgataagaac cggcctttac ctcaaaccgc ttgtatatgg tcgagacgcc gtccttggac
     2221 agaccggtag ggtgcatcac gctgcggaaa aacggctgcc cgtccagatc gacttccaga
     2281 ctgatgtcgg aacgatgcct tggacaatcg agtggcgcgc gcatatttgg cggcattttc
     2341 tccagttctt ccggggtgcg tttacggcat tcgcggccgc ccaggtgact catcgagagc
     2401 ttgatcaacg ccgaatcatc cggaacctgc cgatagaggg ggtaggtcgc aaaatagccg
     2461 atgaaaacgg caaatgcgcc ataaagaacg atctggccga cgatggccac gggcttaagc
     2521 atgatgagta tttctccgaa catagggtag caacgggtcg tcgaaatcct tctggatttc
     2581 aattctgtct ctgaattcac acaaggcctc ggccagcact ttgttgtcat tgaccgaggc
     2641 aaacacctgc tgcactctgg ttataggcac cttgctacgc aagtgaggtt cacgccgtcc
     2701 gctcagccgt tcggctgtcc agcgatcacc caggcgatac tcgcagccgc catcacggca
     2761 actggcaacc agaacgccat cggcaccgcc gcgcagtgca tattcaacga acgccgggga
     2821 aagttcgccc gtgcacagga ggctgatcac ggccgtatcc ggtgcggcca gcgcgtcggc
     2881 gttggcaccg catttgcagc caaagacaac aatccgggtg tgacccacca gtcgggccag
     2941 atggtcttcg agctgccggc gcaagtcgtt gacggactgt tgcggcatgt caatgccggt
     3001 caccaagcgc tcctgcctgc gaaaaggagt cgatgagggg catgagcctg cacagattcc
     3061 gcaacctgca caaagctcgg aagcgacgat agccaacttg taacccggcc gatcgggatg
     3121 cggccccatt gtaattgcgg cgtaggggca gtcggcaagg caacggctgc aaccgttgca
     3181 gttggtcgcg tcaacgacgg cgatgggctc gacctttggg tggggcagga acggaaggac
     3241 gaacaggagg atcgtggcag cgaacagcat cacccagaca atggctggcg acgtggcgtc
     3301 ggtcagcgga tggatgaaca gcaggaacca gtctaggcgg agttgcgcgg ggaccactgc
     3361 cagattcgcc ggagcgtcac tgagcgccgg tttgatcagc gcgagcagca ggagtgcccc
     3421 caacgtgctc agcatcacct gtcgcgacgg aaggtaatcc acgcgactga tgcgatgaac
     3481 atgggcccac aaacccagga taagcatgag cggaattccc aagtggatga agaccagcat
     3541 ggtgaagaat cggtcgttca gcgaacccgg cgacagaaaa ttgcgtgccg cgggttcgct
     3601 gaagatgggt agccagtcga gcagttcggt gctggcggtt gccgagaact gtgcaagctg
     3661 atcccacaca atccagtaac cactgattcc ggaaacgtag ctcagccaga ggatgggcac
     3721 ccctgttatc caggaataga agcggaaacc gtagtagcgt ccgaacgccc attcccgcaa
     3781 cagatgcagc accatgacca gaatgaaggc gtccgacgcg tatcgatgca ggctgcgcag
     3841 aatacccccg agataccact gatcactgga gagatagccg atcgagttgt agacgccgtc
     3901 tatgctggtg tcaagaaccg tgtagagata gagtccggtc ccgacaatga tccagagaaa
     3961 gtacaagccc atggcaccga gatggcgcag cgggttgttg ttccctccaa aatacacatc
     4021 gaaaaactct tcggcgcgct ggaagagagt ttgcgcagta ttgcgcaagg aagcatgaaa
     4081 actcacgtaa aagtgctccg taccgcggag gcaagcataa aaattagccg ttgattatat
     4141 tcttctgggg cagcaaacgt cttgacccgt gtcaatttct tggaagatac cgcgcgtgcc
     4201 atggcgccgg gtccaagcga attacttttc gcttcggcgg cggttcttcc agaagttgaa
     4261 gctcaggtag atcatgaacc ccacgaaagt cagaaaaccg gcaatctgaa aatagaggga
     4321 ataattggtg cggtatcttc cggtcaccgg atcgtagacc gagcagatga ttctgacgcg
     4381 cgccatgatc tcctgcaggg tgccgacttg tgggggaatg ggcgaaccgg tgatcatcac
     4441 tttgagcggt tcagccaggt cttcagcggt aaacttctcg ccgtatacct gccggatgat
     4501 cttgccctgc gcgtcgacca tggtgacttg attcagatgg tcgaacccgg cgggtgtagc
     4561 gacgtaactg aaaccgaaat tgcgggtgag ttcgtcgagt attgctggcg atggactcaa
     4621 aaactcccag ttgggaagat tgatgccgta ttgcgctgaa aagtccttga gcgcctgtgg
     4681 ggagtcgaac ggctggttga agccgatgct gatcacgtta aaacggtcag ctccgagaac
     4741 gctgaccgta tttcgcacgg ccttctggag attgcgggtg gtcgtcgggc aaacctggaa
     4801 gcaggccgta taaatgaagc tgaccagcag cggtttcccg cgatagcgtg agagttctac
     4861 gggtttccct gagcggtcga gcagaacgaa gtcgccgacc ggtttgccga tcacgctttg
     4921 cgagagacca aaggccgcct tctcatccag tgtggtaatc gccaaccccg tattcagtcc
     4981 ctgcgggcgg gcggtcaact tggggttttc cggcgctgag cctgaatccg ctgcaacgag
     5041 gggagctgct gccaaggcag atacggccgc aaagagcgcg acgcaaacct tgaaagtcac
     5101 tgagaacgcc cgcaaactaa aactgggcgt cgataattgc accgaggagc accagcgtta
     5161 actggattag cgaggcgtgg aaacaggtca tggccgtctt acggttggtg tcccgagcca
     5221 atagccaggc cttctggatg aacagcaggc caccggtaac ggcgccggca aagtagatgg
     5281 caccgagccc gaatgccagc ggcaatagag acgcagccac cagcgccaag gtgctgacaa
     5341 gaatgacttt cgccgctcgg ttgtccccga cgaccaccgg cagcatgggg acaccgatcg
     5401 cagcgtaatc ggcccggaaa gcgatggcca gactccagaa atgcggggga gtccacagaa
     5461 acagcacgaa agccagagcg agcggtactg caccaatatg cgggtcagcc gccgcagcgc
     5521 cagcaaggac tgcccagcta cccgccagtc caccaaagac gatattgagc caagtgtggc
     5581 gcttcagcca gacggtataa acgatggcgt agaagaaagc ccccagaaag acatacaggg
     5641 cggaccatgc attgagagcg atccatgcag cacctacgga aagcagcgac aatgcgccaa
     5701 tgatcagcaa ccagaccggg ccactaccga tctcgcccgt cgcgaaagga cgtttgctgg
     5761 tacgcgccat caaggggtca agatcgcgct cgtaatactg gttgaaggcg ccggcgctgg
     5821 ccgaggatat cagcacggaa agcgccagaa cgacgatctg ccaccagctc agtccagcgc
     5881 cggggctgac ggccagcccg gccagcgccg taaaagtaat gacgacgcca atgcgcaact
     5941 tgaacacacc cagaatggta cgaactgaaa tgccggcagc gcggttggcg gggggcgttg
     6001 attgcatggg cgtaagcaaa gtagtgctca agaaaattcg tcgaggtggg aacttgcttg
     6061 aatcgggccg tactgtcaag cccgactgaa gttggccccc acgccatggg cgtggggaaa
     6121 ttctcccaag ttaactcaac aaccagactt cggacagata cttccagtta acaaagtagt
     6181 agagaacgaa ggtaaccagc agcgtcagcg ccaataccac tgtgccaggt acggcaacgt
     6241 ggtcaccctt gtccgtatgc agcacggccg aagcggcttc ggtattcgac gggatcggcg
     6301 ttggcttgtc tgaaaccttg ccgccatcaa gcttcttgcc gaacagcaag gagcctacga
     6361 cgagcagtat ccacagcccg ccacccagaa cggcaatgac accgaatatg ccggtcagac
     6421 ccatcatcag gtacgccgca cccggccatt cgtactggaa cggagcaccg gagaatgcca
     6481 tgtcccagtg acgccgtgaa acgcccagcg tcccggcgcc catcatcacc agaccgagta
     6541 ccgacatgct gattccgtag aggtacggct gaatctggca cagcttggga cagatcattt
     6601 cgcgccggaa cagcgtcggc accagaatgt aggtgatcga catgaaggcc agcgtcgtac
     6661 cgaccacgac cgtggtatgg aagtgccctg gaacgtagaa ggtgttgtgc atcagcatgt
     6721 tgatctgctc ggtgcccagc accacgccgg aaatgccgcc gaggaaaccg aagccgacca
     6781 gcgaaatgaa catgcccgag aaggccgggt taccccatgg tgccttgcgc aaccactcga
     6841 agaggccgtt ggtgtagccc tttctgcgtt gtgcggcctc gatggcgcca ggtacggtaa
     6901 aaccgtggat catcgacgcc atgaccgcga agtacatgaa gtaggacgtg ttcagcacct
     6961 tccattctgc actcatgcca ggatcggaca gcaaatggtg cgcactggcc agttgcagga
     7021 agaggatgta gagcaggaaa gcgaagcgtg acaccttttc cgaaagcggc ttggcgttga
     7081 aaagcacggc cgccgtcaga taccagatcg agacgtgagc cgccacattg atctgctgcg
     7141 aggagtgtcc caggccccac cacaccatgc ggtacaactg ggcatcgatt tccttgatca
     7201 ggccaacaga ccacaggaag gtcgggatca gaatgccggc acccgccaga atggtgaaag
     7261 tggcgatgac ggccgcggtc atggcaccga aggtgaccaa tggcagcgaa ccctggtaag
     7321 tcttgtcctt cttggcgacg accagcgtgg caaagaagat gccggtgccg atgagcgcac
     7381 cgaccgctac caggatgatg ccaaggtaga agtgcggcgc agcctgcatc ggcacatagg
     7441 aggtgaacat cacgctcgac tcgccctgga aaatggcgac gttgcagacg acaccaccga
     7501 tgaccatcag ccagaagctc agccaggcca gtttaggggt cgccagtcgg cagcgaagca
     7561 gagtggacga ggcaaagaag agcaccgcca tctcgaaatg cagtatccac aggatcagca
     7621 tgtcgatgcc gtgcgcagta agaatcaggt agaactggtc tgcgggcagc aacttgatcg
     7681 ccggccagcg ggtaagaatc acgaacagtg cggtgatgcc gccaaccagc agccagagca
     7741 cgccgactac ggcgtgccag cgcatcagtt tttccgcgtt gttttcaaat tgcagtccgg
     7801 agctcggaca agtgcggtaa gtagttgcca tctaggtgct ccccttattt cacgtagatg
     7861 cggccaagca tggagtggtg accaatgccg cagtattcgt tacagacgat ggcgtaggtg
     7921 ccttctttgg tcggcgtcat cttgaccaca tgctcgtaac ccggaatcac ctggatgttg
     7981 atgttgaccg gctgtagcga gaagccgtgg ttgtagtcca tcgacgaaag atgaatcttg
     8041 taggtctggc ccttttccag ctcaagaatc gggtaccagt tccagagacg ggcgatcagg
     8101 aagccatcgc cgccggccgg caccttgacg accggaatct gctggccgtt gagatccgtt
     8161 tccgagcgga ctgtgttttc agcgatgaac ttttcggctt tggcggcgaa catttcgggc
     8221 gtggtcttgt aggcctcgtt cgacaggttc tgcttaccgt agatatgcca atagaccatc
     8281 atcacgaa
//
LOCUS       DAORPM010000096         8227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2129_length_8227_cov_10.890113, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000096 DAORPM010000000
VERSION     DAORPM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_8227_cov_10.890113"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLD53_06190"
     CDS             <1..518
                     /locus_tag="PLD53_06190"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRC60537.1"
                     /translation="PEVSAEQRLEMVRLAISGNPRFELDSAEVNDARPSYTVPTLERL
                     RTTDEFGPQRPLVLLVGADAFSGMAGWHRWESLFDLAHIAVAHRPGFPVNAASLPTQV
                     AAAYQQRLSSSPAALQDAPAGRIVTFAMTQLAVSATQIRTLLLNGRSPRYLLPEAVIA
                     YIQSTQLYSKR"
     gene            535..897
                     /gene="rsfS"
                     /locus_tag="PLD53_06195"
     CDS             535..897
                     /gene="rsfS"
                     /locus_tag="PLD53_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRC60538.1"
                     /translation="MNITQLQKIVVDALEDIKGRDIEVINTSKLTPLFERIIIACGDS
                     NRQVKSLARNVQDKVREADGQIISTEGEESGEWVLVDLGDIVVHVMQPAIRSYYNLEE
                     LWGGKGPERVRRVAAAGA"
     gene            894..1364
                     /gene="rlmH"
                     /locus_tag="PLD53_06200"
     CDS             894..1364
                     /gene="rlmH"
                     /locus_tag="PLD53_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRC60539.1"
                     /translation="MRLGILAVGHKLPEWVAKGCAEYTKRLPHELPLSVVEVKPEPRG
                     SKTREQLLAAEKLRLQNALQSFSRKVVLDEKGADLTTLELARRLEGWMREGGDTAFII
                     GGADGIDETLKRQADDTIRLSSLTLPHAMARLVLCEQLYRAASVIKNHPYHREG"
     gene            1361..1990
                     /locus_tag="PLD53_06205"
     CDS             1361..1990
                     /locus_tag="PLD53_06205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HRC60540.1"
                     /translation="MMVPISQQHPRIYLASRSPRRRELLTQIGVQFDTIVLRNHPRED
                     RETDEVPLDGEGPTEYVERVARTKAEQGWRIVSWRKLISQPVLAADTTIEFEGRILGK
                     PIDEEDAKYILRRLSGNVHRVLTAVAVAFEGRIESVLSVSEVRFGVIDEAEIRRYVMS
                     GEPLDKAGAYGIQGRAGLYVEHLTGSYSGVVGLPLFETGVLLKRFNFPL"
     gene            2214..2290
                     /locus_tag="PLD53_06210"
     tRNA            2214..2290
                     /locus_tag="PLD53_06210"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2248..2250,aa:Gln,seq:ttg)
     gene            2381..3331
                     /locus_tag="PLD53_06215"
     CDS             2381..3331
                     /locus_tag="PLD53_06215"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRC60541.1"
                     /translation="MAYDSLMVFTGNANPKLAADVVKRLNISLGRAKVGRFSDGEITV
                     ELQEHVRGKDVFILQSTCSPANENLMELLVMVDALKRASATRITAAIPYFGYSRQDRR
                     VRSARVPIAAKLVADLLTAAGVHRVLTMDLHAEQVQGFFNIPVDNIYSLPIMLADVWK
                     KDIDGLMVVSPDVGGVVRARALAKRLECDLAIIDKRRPKANVSEVMNIIGEVEGRTCV
                     IMDDMVDTAGTLCKAASALKNKGANLVLAYCVHPILSGTAVSRINDSEIDELVVTDTI
                     PLSDEAQKSPRIRQLSVADVLAETIRRISNEDSVSSLFIE"
     gene            3388..4026
                     /locus_tag="PLD53_06220"
     CDS             3388..4026
                     /locus_tag="PLD53_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRC60542.1"
                     /translation="MKFELNAQKRTLQGSGASRRLRRANKVPGIVYGGTAVPTLIEIE
                     HNDLLLSLRKEAFHSSVLTIKIDGVAESVLLRDSQMHPYKPLVQHVDFLRVDATHAIH
                     QKVPLHFLNAEVSPGVKLAGGNVSPAMNDIEVSCLPQDLPPFIEVDLKDMEVGQSIHL
                     SQIVFPKGVAPTAHTDDDPVVVTISAKKAEVVEEGEGEREGEGEGEAATPAA"
     gene            4221..4790
                     /gene="pth"
                     /locus_tag="PLD53_06225"
     CDS             4221..4790
                     /gene="pth"
                     /locus_tag="PLD53_06225"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRC60543.1"
                     /translation="MTSPRLIVGLGNPGSEYADNRHNLGFWFIERLASDLKVSLTPQG
                     KFFGVVARVGELSLLQPTTFMNRSGQSVAALARFYKLMPEEILVVHDDLDLQPGGIRL
                     KKGGGNGGHNGLRDIQAHLSTPDFWRLRVGIGHPGERSEVVNFVLQAPRREERELIDH
                     ALDRCLLAWPKLCAGDYAAAQRLLHSKAT"
     gene            4817..5908
                     /gene="ychF"
                     /locus_tag="PLD53_06230"
     CDS             4817..5908
                     /gene="ychF"
                     /locus_tag="PLD53_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRC60544.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGVAAENYPFCTIEPSVGVV
                     EVPDPRLEKLSAIVKPQRVVPAVTEFVDIAGLVAGASKGEGLGNKFLANIRETDAVLH
                     VVRCFSNDNVIHVSGNVDPIRDIEIIDTELALADMATVEKALLRYRRPASAGDKEAKL
                     LVAVLDTCFAQLDQGKPIRALDLSKEEWSNLKPFCLITAKPVLYAANVAEGGFENNPH
                     LDAVRAHAATERAEVVAVCASIEAEIADLDDDEKQLFLADLGLEEPGLDRLVHAGYHL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQTIAYDDFIACGGEH
                     GAKEAGKMRAEGKEYIVKDGDVLNFLFNV"
     gene            complement(6022..>8227)
                     /locus_tag="PLD53_06235"
     CDS             complement(6022..>8227)
                     /locus_tag="PLD53_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC60545.1"
                     /translation="WITFADDGRRVLVETTKTPMHDATGKLIGVLGIARDITRRNQAE
                     QAVRESEERYRVAFRTSCDSVNINRIADGCFVDSNEGFERMTGWRREEVIGRSSLELG
                     IWVDPEDRRRMVEKLRQDGFCENMQFRFRRKDGSELIGQMSVHAIPVDGDECLLSVTR
                     DITEQVRIEEALRQREQYQRALLDNFPFLVWLKDNSSRFLAVNKPFAEACGQPSPNHL
                     VGKSDLDIWPRDLAEAYQADDRAVLESGRPTNVEEPIEVGGKRIWFETYKSPVLIDEH
                     VIGTVGFARDITERKATEEEVRRLAFYDTLTHLPNRRLLVDRLQQAIASSARSGKQVA
                     VLFIDLDNFKILNDTLGHGIGDLLLQQVAKRLEHCVREGDTVARLGGDEFVVILEDLS
                     GEVFEGARQVEIIGEKIHAELVRPYDLDGHQTNSTPSIGITLLTDRETTFEELMKRAD
                     MAMYQAKGAGRNTLRFFDPEMQAAVAARTALEGDLREGLKQGQLLLHYQPQVDGSGTV
                     TGAEVLVRWRHPLRGLISPIEFIPLAEETGLILPLGAWVLTTACNQLVSWSSDPAMAN
                     LTLAVNVSARQFRQPDFVGQVLTTLETSSANPTKLKLELTESLLLDNVEEIILKMNAL
                     RSSGVSFALDDFGTGYSSLSYLKRLPLNQLKIDQTFVRDVLSDPNDAAIARTIVALAQ
                     SLGLSVIAEGVETEEQREFLAVSGCHACQGYLFSAPLPLEEFRQFVSRKSAS"
BASE COUNT         1659 a         2350 c         2405 g         1813 t
ORIGIN      
        1 ctccggaagt gagtgccgag caacggctcg agatggttcg cttggccatt tccggcaacc
       61 cgcgtttcga gctggactcg gcagaggtca acgatgcccg accaagctac accgtgccga
      121 cccttgaacg cctgcgaacg accgatgagt tcgggccgca gcggcccctg gtcctgcttg
      181 ttggggctga cgctttttcc ggcatggcag gctggcatcg ttgggaatcg ttgttcgatc
      241 tggcgcacat tgcggtggcg caccggcctg gcttcccagt caatgcggcc agcctgccaa
      301 cacaggttgc cgcggcttac cagcagcgcc tcagtagttc acccgcagca ctccaagatg
      361 ctcctgccgg tcgtatcgtg accttcgcca tgacccaact ggccgtctcg gccacacaga
      421 tcagaacgct gctgctcaac ggtcgcagcc cgcgctattt gctgccagaa gcggttatcg
      481 cttatattca gagtactcag ctttactcca aacgatgaaa cccgcccaat cctgatgaac
      541 attacccaac tgcagaaaat cgtcgtcgac gcccttgaag acatcaaagg tcgtgacatc
      601 gaagtcatca acaccagcaa gctcacccca ctttttgaac gcatcatcat tgcctgcggc
      661 gactccaacc gtcaggtcaa gtcacttgcg cgcaacgtgc aggacaaggt gcgcgaagcg
      721 gacggccaga tcatttccac cgagggtgag gaaagcggtg aatgggtgct ggtcgatctc
      781 ggggatatcg ttgtccatgt catgcagccg gccattcgca gctactacaa ccttgaagag
      841 ctgtggggcg gcaagggccc tgaacgtgtc cgccgggttg ccgccgctgg cgcatgagac
      901 tcggtattct tgctgttggc cacaagcttc ccgaatgggt agccaaaggt tgtgccgagt
      961 ataccaagcg cttgccgcat gaattgccgc tctccgttgt cgaggtcaag cccgagccgc
     1021 gcggttcgaa aacgcgcgag caacttctgg ctgccgagaa gcttcgtttg cagaacgcct
     1081 tgcagagttt cagccggaag gtcgtactcg atgagaaggg cgccgatctg accaccctgg
     1141 agctggctcg acgcctggaa ggctggatgc gcgaaggcgg tgataccgcc ttcatcatcg
     1201 gtggcgccga tggcatcgat gagacattga aacggcaggc cgacgacacg attcgtcttt
     1261 ccagcctgac actaccgcac gccatggcac ggctggttct ctgcgaacag ttgtatcgcg
     1321 ctgcaagcgt gatcaagaac catccatatc atcgggaggg atgatggtac ctatcagcca
     1381 acagcatccg cgtatctatc tggcgtcacg cagcccacgc cgccgcgagc tgttgacgca
     1441 gattggcgtt cagttcgaca ccatcgtctt gcgtaaccat ccacgcgagg acagggagac
     1501 cgatgaagtg ccgctggatg gcgaaggccc gaccgagtac gttgaacgcg tcgcacgaac
     1561 caaggccgag cagggctggc gtatcgtcag ttggcggaaa ttgatatcgc aacccgtgct
     1621 tgctgcggat acgacgattg aattcgaagg ccggattctg ggtaagccga ttgatgagga
     1681 ggatgcaaag tacatactgc gccgcctttc gggcaatgtg caccgtgttc tcactgccgt
     1741 cgccgtggcc tttgaagggc gtatcgaatc ggtgctttcg gttagcgaag tgcgttttgg
     1801 cgtaatcgac gaggctgaaa tacggcgtta tgtaatgagt ggtgagcctc tggacaaggc
     1861 cggtgcctac ggtattcaag gccgtgccgg cttgtatgtc gaacatctga ccgggagcta
     1921 ttcgggcgtc gtgggtttgc ccttgtttga gacaggggtt ttgctcaagc gcttcaattt
     1981 cccgttgtga ccttgtgagc gatctgtcac ctggggcccg cttcgaatag cctcccaagt
     2041 agcgctgctg tcgagtaatg cggctgcgcc ctcatgaatc gcgctttctg ggctaatcct
     2101 gtctgttggc ccgtgtattg gcgccctttg gcgcacgctt cgccctgtat tttttgggca
     2161 aacacctgta taatccgcgc ctcgttcgtc aggctccaaa tgcgagcgac tgctggggag
     2221 tcgccaagtt ggtcaaggca ccggattttg attccggcat tcgaaggttc gaatccttct
     2281 tccccagcca ttcggttttt tcaagccggg cgggtagtca gcctacctgc ccgtattgct
     2341 ttatatgcag cgtcttgccg acgctacggg gatgcgcaat atggcctacg acagcctgat
     2401 ggtcttcacc ggtaacgcca accccaagct ggccgccgat gtcgtcaaac gcctgaacat
     2461 ttcgcttgga cgggccaaag tggggcgttt ctccgatggc gaaatcacgg ttgagttaca
     2521 ggagcacgtg cgcggcaagg acgttttcat actgcaatcg acctgttcgc cggccaacga
     2581 aaacctgatg gagttgctgg tcatggtcga tgcgctaaag cgcgcctcgg ctacccgcat
     2641 tactgcggcc ataccttact tcggatactc gcgtcaggat cggcgggtgc gctcggcgcg
     2701 cgtaccgatt gccgcaaaac tggttgccga cctgctgacc gccgccggtg ttcaccgtgt
     2761 gctgaccatg gatttacatg ccgagcaggt ccaggggttt ttcaatattc cggtggacaa
     2821 tatctactcg ctgccgatca tgctggctga cgtgtggaaa aaggacatcg atggcctgat
     2881 ggttgtttcc cccgatgtcg ggggtgtggt tcgcgcgcgg gcgctggcca agcgccttga
     2941 gtgcgatctg gccattatcg acaaacgtcg tcccaaggcc aacgtctctg aagtaatgaa
     3001 catcattggc gaggtcgaag gccgtacttg cgtgatcatg gatgacatgg tcgatacggc
     3061 aggcacgctg tgcaaggccg ccagcgcgct caagaacaag ggcgccaatc tggtgctggc
     3121 ctactgcgtg cacccgatcc tctccgggac agccgtcagc cgcatcaatg actcggaaat
     3181 cgacgaactg gtggtgaccg ataccatccc actgagcgat gaggcgcaga agtcaccgcg
     3241 tatccgtcag ttgtcggtgg ccgatgtgct ggccgaaacg atacgtcgta tcagtaacga
     3301 ggattccgtt tcctcgctat ttattgagta gttttatcaa ccaccctgtc tggtcgcggg
     3361 caggggctgt ttcaaccagg agccaaaatg aaatttgaac ttaacgcgca gaagcgcaca
     3421 ttgcaggggt ccggagcgag ccgccgcctg cgtcgcgcaa acaaggtgcc gggcatcgtt
     3481 tacggtggga cagccgtccc gaccttgatt gaaatcgaac acaacgatct tcttttgtcc
     3541 ttgcgcaagg aagcatttca ctcgtcggta ctaacgatca agatcgatgg cgtcgctgaa
     3601 agcgttctgc tacgtgattc gcagatgcat ccctacaagc cgctggttca gcatgtcgat
     3661 tttctgcgtg ttgacgctac ccatgcgatt caccagaaag ttcccttgca ctttcttaat
     3721 gccgaagtat ctccgggggt caagcttgcc ggtggcaatg tatcaccggc aatgaacgac
     3781 atagaagtca gttgtctgcc gcaagatcta ccccccttca tcgaggttga tctgaaggat
     3841 atggaagtcg gtcaatcgat ccacctttcg cagattgtgt ttccgaaggg tgttgcgccc
     3901 acggcacaca ccgatgatga tccggtcgtg gttacgattt cggccaagaa ggcggaagtt
     3961 gttgaggaag gcgaaggcga acgtgaggga gagggagagg gcgaagccgc cactcctgct
     4021 gcctgatctt ctgcatcaag acgaaaggcc gccgtttgga gcctggctcc ccggcggccg
     4081 tttcgttcag cagttctgac gcgcgatcga cggcaaaggc acgcaacagc attgagctcg
     4141 acaatttggc cgcgccagat gacttccgtg ttcccttgtt catttgtact catgagttga
     4201 gtttcgggtt ctaaaacacc atgacctctc cccgcctgat tgtcggcctc ggtaatcccg
     4261 gaagcgagta tgcggataac cgtcacaatc tcggtttctg gtttatcgaa cggttggcga
     4321 gcgatctcaa ggtgtcactt acgccgcagg gcaagttctt tggcgtcgtc gctcgcgtgg
     4381 gtgagctgag cctgctgcaa ccgacgacct tcatgaaccg ctccggacag tcagtcgccg
     4441 ctctggcgcg attctacaaa ctcatgccgg aagaaatact ggtcgtgcat gacgatctag
     4501 acctgcaacc gggcggcatc cggctcaaga aaggtggggg taacggaggg cataacgggc
     4561 tgcgagacat tcaggcgcac ctgtctaccc cggatttctg gcgcttgcgc gtgggcatcg
     4621 ggcatccggg tgagcgcagc gaggtcgtca atttcgtcct tcaggcgccg cgtcgcgagg
     4681 agcgggaatt gatcgatcac gcgctggatc gctgcctgct ggcctggccg aaattgtgtg
     4741 ccggtgacta cgctgcggcg caacgcctcc tgcacagcaa agccacataa caattgtcac
     4801 gaacaggaat ttcaccatga gtctcaaatg cggaatcgtc ggtctgccca atgtcggcaa
     4861 atccacgctg ttcaatgcct tgaccaaggc aggtgtagcg gcggaaaact accccttctg
     4921 taccattgaa ccttcggttg gtgttgtcga agtgccggat ccgcgcctcg aaaagctgtc
     4981 tgcgatcgtc aaaccgcagc gcgtggtgcc ggcggtgacc gagttcgttg acatcgctgg
     5041 tctggtggct ggtgcctcca agggggaagg gctgggcaac aagttcctgg ccaacattcg
     5101 cgagacggac gccgttctcc atgtcgtccg atgtttttcg aacgacaacg tgattcacgt
     5161 ctcaggaaat gtcgacccta tccgcgatat cgaaatcatc gacaccgaac ttgcgctggc
     5221 tgatatggcc acggtcgaaa aggcgctgct gcgttatcgc aggccggcca gtgccggtga
     5281 caaggaagcc aagcttcttg tcgccgtgct ggacacgtgt tttgctcagc ttgaccaggg
     5341 caagccgatt cgtgcactcg atctctcgaa ggaggagtgg agcaatctca aacctttctg
     5401 cctgattacc gccaagccgg tactgtatgc cgccaacgtt gccgagggcg gcttcgaaaa
     5461 caacccgcat ctcgatgcgg tgcgggctca tgctgcgacc gaaagggccg aagtggttgc
     5521 cgtatgcgct tcgatcgaag ctgagattgc agatctggac gacgacgaga aacaattgtt
     5581 tctcgccgat ctcggtcttg aggaacccgg gctcgaccgt ttggtgcatg ccggctacca
     5641 cttgcttggc ctgcagacgt atttcaccgc gggtgtcaag gaagtgagag cgtggacgat
     5701 ccacaagggc gatacggcac cgcaggctgc cggagtcatt cacaccgact ttgaacgcgg
     5761 tttcatccgc gcgcagacga ttgcttacga cgatttcatt gcctgtggcg gcgagcatgg
     5821 cgccaaggaa gcgggcaaga tgcgcgctga aggcaaggag tacattgtca aggatggaga
     5881 tgtccttaat ttcctattca atgtttagca gctcatgcca agcctttgaa gaagtgttcc
     5941 cacgcagtgg ccatgaattg gcacgttttg cctgcgacat cccgtgccca gggaagtgat
     6001 cgtatcgtcc gtgtatcaca cctatgacgc cgatttgcgg ctgacaaatt gccggaactc
     6061 ttccagtgga agcggagcac tgaacagata gccctgacag gcgtggcatc cggaaacggc
     6121 aaggaattct cgctgctcct ccgtttccac tccctcggcg attaccgaaa gccccaggct
     6181 ctgtgccagc gcgacgatgg tgcgagcaat ggccgcgtca ttggggtcac tcagtacgtc
     6241 acgtacgaac gtttgatcaa tcttcaactg gttcagtggc agtcgtttca ggtatgacaa
     6301 cgacgaatag cctgtgccaa aatcatccag cgcaaagctc acaccggaac tcctcagcgc
     6361 gttcatcttg aggatgatct cttcgacgtt gtcgagcaac aggctttcag tgagttccag
     6421 cttgagtttc gtcggattgg cgctcgatgt ttcaagtgtg gtcaatacct gccccacaaa
     6481 atccggctgg cgaaactgtc gggcgctgac gttgaccgcc aaagtgagat ttgccattgc
     6541 cggatcgctt gaccaagata ccaactgatt gcacgcggtc gtcagcaccc aggcgccgag
     6601 cggcagaatc agtccggttt cctcggccag aggaatgaat tcgatggggg aaatcaagcc
     6661 gcggagggga tgccgccacc tgaccagtac ttccgcaccg gtcaccgtgc cactgccgtc
     6721 tacctgcggc tggtagtgaa gcagcagctg accctgcttc agcccttcgc gaagatcgcc
     6781 ctcgagcgcg gtgcgcgcgg caaccgcggc ctgcatttcc ggatcaaaaa atcgcagggt
     6841 attgcgtccg gcccccttgg cctgatacat ggccatgtcg gcccgtttca tcagttcctc
     6901 gaaagtcgtt tcccggtcgg tgagaagggt gatgccgatg ctcggcgtgc tgttggtttg
     6961 atggccgtcc agatcgtagg gccgaaccag ttcggcgtgt atcttctcgc caatgatttc
     7021 gacttgcctg gcgccttcga atacttcccc gctcagatcc tcaaggatga cgacgaattc
     7081 gtcaccgccc aggcgcgcca cggtgtctcc ttccctgacg cagtgctcaa gacgtttggc
     7141 aacctgttgc agcagcaggt cgccgatgcc gtggccgagg gtgtcgttga gaatcttgaa
     7201 gttgtccaga tcaatgaata gcaccgccac ctgcttgccg ctgcgagcac tcgatgcgat
     7261 tgcctgctgc aggcgatcga ccagcagtcg ccggttgggt agatgggtta gcgtgtcgta
     7321 aaacgccagg cgccggactt cctcctcggt ggctttgcgt tcggtgatat cgcgcgcaaa
     7381 accaaccgtg ccgatgacat gttcgtcgat caggaccggt gacttgtagg tttcgaacca
     7441 tatcctcttg ccgccgactt caatgggttc ctctacattt gtcgggcggc cgctctcgag
     7501 tacggcccga tcgtccgctt gataggcttc agccagatca cgcggccaga tatcgagatc
     7561 ggatttgccg accaggtggt ttggcgaagg ctggccgcag gcctcggcaa acggcttgtt
     7621 gacggcgaga aagcggctgc tgttgtcttt aagccagacc aggaacggga agttgtcgag
     7681 caatgcccgc tggtattgct cacgttgtcg tagcgcctcc tcgatcctga cctgttcggt
     7741 gatgtcgcgg gtgaccgaaa gcaggcattc gtcgccatca acgggaatgg cgtgcaccga
     7801 catctgtccg atcagttcgc tgccgtcctt gcggcggaag cggaactgca tgttctcgca
     7861 aaagccatcc tgtctcaact tttcgaccat gcgccggcga tcctccgggt ccacccagat
     7921 cccgagttcc agtgagcttc ggccaattac ttcctcgcgc cgccaaccgg tcatgcgctc
     7981 gaaaccctca ttggaatcta cgaaacaacc atcggcaatg cggttgatgt tgaccgaatc
     8041 gcaactggtg cgaaaggcca cgcggtagcg ttcttcgctt tcgcgtaccg cctgctcggc
     8101 ctggttgcgg cgggtaatgt cgcgggctat gcccagcacg ccgatcagct tgcctgtggc
     8161 gtcgtgcatc ggcgtcttgg tggtttcgac caggacacgg cgaccatcgt cggcaaaagt
     8221 gatccat
//
LOCUS       DAORPM010000097         8210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2137_length_8210_cov_9.341876, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000097 DAORPM010000000
VERSION     DAORPM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2137_length_8210_cov_9.341876"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLD53_06240"
     CDS             complement(<1..770)
                     /locus_tag="PLD53_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRC60546.1"
                     /translation="MSSLMGAAALMVQSDDVEAAIEWAEHFQKNYRLMTPEEKAEARA
                     RLEKRYSAEYDKKVTVDGTEAQPGVLMGYALNIRKCIGCRRCVHACVEENNQSRGTRP
                     GEKIEWIQVLRMERGEFSQDKMNHGYPEGLGIQVGGNAYSPAGQVLEGQYYYEPEAVP
                     EKDATYMPIACMQCEKPPCVKVCPVRTTFREGDGVVVIDYNWCIGCKMCMNACPYWAR
                     RFNLTTPVLPKQAMNPVTHYMGNRPRMRGVVEKCHWCL"
     gene            complement(1547..1759)
                     /locus_tag="PLD53_06245"
     CDS             complement(1547..1759)
                     /locus_tag="PLD53_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60547.1"
                     /translation="MTKLKPVAILAIATAIIILGSELGEQLSVPGIHGVIPSAEAIVG
                     RPLTPVSYAGVARRTVRRCAVGVYYC"
     gene            complement(1756..2565)
                     /locus_tag="PLD53_06250"
     CDS             complement(1756..2565)
                     /locus_tag="PLD53_06250"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HRC60548.1"
                     /translation="MNLSVRNVLQFSARSLSVLALSVALGLGLSTGASADEADAKRLL
                     KAMSDYLAAQQTLSFSYDATLEVVTAEDQILGIASSGMVALKRPDKLHATRSGGFADV
                     DMTFDGKTLTILGKNLNKYTQIEAPGSIGQLVDLLRDKYGRPLPAADLLLTNSYDELM
                     RDVVDIKDLGSGVIGGVECDSLAFRSKEVDWQIWIAHGDKPYPCRYVITSKSMPNGPS
                     YSIQIRDWKTGKAVAASSFEFKNTSKASMVKLEDLKGTGDLPEHFTKGERK"
     gene            complement(2701..3105)
                     /locus_tag="PLD53_06255"
     CDS             complement(2701..3105)
                     /locus_tag="PLD53_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60549.1"
                     /translation="MTLNHLINILALTTLSGTLTACSLFGSQPATSSTRYEVVKGRQQ
                     QLNFKLASGIYRCELGQKVEVQRDARNANLIEIDWQRSHYTLQRYDSTSGLPRFEDRK
                     NGLLWIDLPWKSVLMDTSSGRPLANECKAMTS"
     gene            complement(3257..4186)
                     /locus_tag="PLD53_06260"
     CDS             complement(3257..4186)
                     /locus_tag="PLD53_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC60550.1"
                     /translation="MNRTVIAYLALLVLGIVLPFFVYPVLVMKVLCFALFACAFNLLL
                     GYTGLLSFGHAAFFGTAAYVAGYAIKGWGFPPELGILAGAAASALLGWVVGSLAIRRT
                     GIYFAMVTLALAQMVYFFFLQAPFTGGEDGLQGVPRGKLLGLISLANDLNLYYFVLAI
                     FVFGFCLIYRTIHSPFGQILKAIRENEPRAISLGYDVAKYKLLAFVLSAGLAGLAGAT
                     KMLVFKFATLTDAHWHSSGEVVLMTILGGMGTVLGPVVGATVIVSLQNELADKVGSLV
                     TVIMGTIFVICVLLFRRGIVGEISALYKRMVGS"
     gene            complement(4183..5070)
                     /locus_tag="PLD53_06265"
     CDS             complement(4183..5070)
                     /locus_tag="PLD53_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC60551.1"
                     /translation="MTALFDIPIQALMGQLMLGLVNGSFYAVLSLGLAVIFGMLNIIN
                     FAHGALYMMGAFLAWMGLEYMGIGYWPALILAPLAIGVFGVLIERGMLRWLYKLDHLY
                     GLLLTFGLALVIEGLFVNAYGVSGQPYQIPAQLAGGFNLGFMFLPYYRGWVIFASLMV
                     CFATWYGIEKTRLGAYLRAATENPQLTQAFGINVPIMVMLTYGFGAALAGFAGVLAAP
                     VIQVSPLMGSHLIITVFAVVVIGGMGSILGSIITGLGLGVIEGITNLLYPAASNTVVF
                     VIMAIVLMVRPAGLFGKEK"
     gene            complement(5120..6352)
                     /locus_tag="PLD53_06270"
     CDS             complement(5120..6352)
                     /locus_tag="PLD53_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC60552.1"
                     /translation="MKYKLLNVALAALLIPAANFASAEAKPAAMNISGNMVKIGVLTD
                     MSGVYSDLGGQGAVTAVQMAIDDFKAQYKPKFAIEMVYADHQNKADVGSNKVREWYDT
                     EGVDMVTDLLNSGVSLAVGKVTQEKKRIAIFVGSGSTRLTDQDCTPNTVHYAYDTYAL
                     ANVAGKAIVKNGGDTWFFLTADYAFGHSLEKDTSDVVKANGGKVLGSVRHPLNASDFS
                     SYLLQAQASKAKIIGLANAGGDTINSIKAASEFGITKNQQLAGLLMFITDIHALGLQT
                     TQGMLLTSGFYWDKDDETRKFSKRFFDKTKRNPTMVQAGDYSAVTTYLKAVMAAGTDE
                     AAAVMAKMKSTPINDFFAKNGKIRENGRMIHDMYLMQVKKPSESKYPWDYYDIKAVVS
                     GEDAFQPLSASQCPLVKK"
     gene            complement(6411..7121)
                     /locus_tag="PLD53_06275"
     CDS             complement(6411..7121)
                     /locus_tag="PLD53_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC60553.1"
                     /translation="MTSAVEYLRISDLHAFYGESHILHGMDLRVNRGECVSLLGRNGA
                     GRTTTLRAILGLTGKRTGSIMLNGRETVSMATHRIAQLGVGYCPEERGIYASLSCEEN
                     LLLPPKVGSGGMRLDEIYAMFPNLAERRHSQGTRLSGGEQQMLAMARILRSGAQLLLL
                     DEISEGLAPVIVQQLGQVIGDLKSKGFTIVLVEQNFHFAAPLADRMYIVEHGQIAAEI
                     AQNEIETKQELLQEYLGV"
     gene            complement(7118..7891)
                     /locus_tag="PLD53_06280"
     CDS             complement(7118..7891)
                     /locus_tag="PLD53_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC60554.1"
                     /translation="MNDYILETDNLVKEFKGFVAVDNVSLKVRRGHIHAMIGPNGAGK
                     TTCFNLLTKFLQPTRGAIRFNNIDITGDPPAKIARRGIVRSFQISAVFPHLTVLENVR
                     IALQRKLGGSFYFWRSDKTLSRLDTRAIELLAEVGLDAQNRMVAVELPYGQKRALEIA
                     TTLALEPELILLDEPTQGMGHEDVDRVMQLIKKVSADRTILMVEHNMKVVAGISDTIT
                     VLARGSVLAEGPYAEVAKNPQVIEAYMGTAENEASGSQA"
BASE COUNT         1680 a         2352 c         2440 g         1738 t
ORIGIN      
        1 tgcaggcacc agtggcactt ctcgacgacg ccgcgcatgc gcggccggtt gcccatgtag
       61 tgcgtcaccg ggttcatcgc ttgcttcggc aggacgggcg tggtgaggtt gaatcggcgc
      121 gcccagtagg ggcaggcatt catgcacatc ttgcagccga tgcaccagtt gtagtcgatg
      181 acgaccacgc catccccttc gcggaaggtt gtgcgtaccg ggcagacctt gacgcagggc
      241 ggcttttcgc attgcatgca ggcgatcggc atgtatgtag cgtctttttc cgggaccgct
      301 tcaggctcgt agtagtattg cccttcgagt acctggccgg cgggggagta ggcgttgccg
      361 ccgacctgga tgcccaggcc ctcgggatag ccgtggttca tcttgtcctg cgagaactcg
      421 ccgcgctcca tgcgcagcac ctggatccac tcgatcttct cgcccggccg cgtgccgcgc
      481 gactggttgt tctcttcgac gcaggcatgc acgcaacggc ggcagccgat gcacttgcgg
      541 atattcagcg cgtagcccat gagtacgccc ggctgcgcct cggtgccatc gacggtgacc
      601 ttcttgtcgt actcagcgga ataccgcttt tcaagccggg cgcgcgcttc ggctttctcc
      661 tcgggcgtca tcagacgata gttcttctgg aagtgctcgg cccattcgat ggcggcctcg
      721 acatcgtcgg actggaccat cagtgccgca gcacccatca gcgacgacat gctgagggct
      781 ccgcccgctt tcagaaagct gcggcgagtg gtcgttatgc caggtgcgga aggatcggct
      841 gacgcaagtt gcgcctggca ttgggaaggc ttttcctgga cgggctcggc tgaggggcgc
      901 tctttagtgg tcacaattgg ctccggtaaa gggtagtgat ttcatgcagg caggtcacgc
      961 ggaccggaat ccaggttggg ctgctgccac tcgggagtca cggtgtgatc gaaaatgaat
     1021 acctcaatca agcagtaggc ggtgcttgcc ctggaagcga tttggagagg tataccagcg
     1081 acgcgcattt ctgtatttga tagtgatcaa tactgctgaa gcaagtcggg ttatatttgt
     1141 gagcttcggc gagaattgag ccgtgtgcga ttgaaagagc atacatcgct gagcgagctg
     1201 gagcaagcgc ccagcgttgc gcccagcgtt gcgagcgacc ccggcctgat cagcttcgct
     1261 gaccattcct tggcagccgc gctacacgga gaccgccaat ttgtacggca acgcacccgg
     1321 aaataatgcg gttctcaggc atgctcttgt cgtcccgact ggcgacccct ggttggttcg
     1381 aacccagccc cttttgaacc caccttctga cctcttgacg cgtggcacaa ctccgtccgg
     1441 gacgggttgt gctggctgat tcaccgttgg ccagcttgcg cgtggcgcct gccttgccga
     1501 aggtggagta gcgtgatgcg gcgctccgtc ttcgggagtt gcttgcctag cagtaataga
     1561 ccccaacggc gcaacgtcgc acggtacgac gggccacgcc ggcatagctc accggcgtaa
     1621 gaggcctgcc gacgatggct tcggcgcttg ggatgacgcc gtgaatcccg ggcaccgaga
     1681 gctgttcgcc cagttcagat ccgagaatga tgattgcggt ggcaatcgcg agaattgcca
     1741 ctggtttcag cttggtcatt ttctttctcc tttggtgaag tgctcgggca agtcacccgt
     1801 acctttaaga tcctcaagct tgaccatgct ggctttgctc gtattcttga attcgaagct
     1861 gcttgcggca acggccttcc cggtcttcca atccctaatc tggatgctgt aactcggccc
     1921 attgggcatg ctcttggaag tgatgacata ccggcaaggg tagggcttgt cgccatgagc
     1981 gatccagatc tgccaatcga cctctttgct gcggaaggcc agtgaatcgc actcgacgcc
     2041 accgatcacg ccactgccaa gatcctttat gtcaacgacg tcccgcatca actcatcgta
     2101 tgaattggtc aggagaagat cagcagcggg aagggggcgg ccgtatttgt ccctaagcaa
     2161 atcaaccagt tgcccaatag acccaggcgc ttcaatctgc gtgtacttgt tcagattctt
     2221 gcccaggatg gtcaacgttt ttccgtcgaa tgtcatgtca acatccgcaa atcctcctga
     2281 gcgagtagcg tgaagcttat cgggccgttt gagtgcgacc atgcccgaac tcgctatccc
     2341 gagaatctgg tcctcggcgg taacgacctc aagggtagcg tcgtagctga acgagagggt
     2401 ttgttgtgcg gccagatagt ccgacatggc cttgagcagt cgcttggcat ctgcttcatc
     2461 agcgctcgcg cctgttgata accccaggcc aagcgccacg gaaagtgcaa gcacggaaag
     2521 gctgcgcgcg gaaaactgta atacattgcg tacggatagg ttcattcggg atacctttat
     2581 gagctgagat actgtgttgg aagccttacg gctgattcag tttagcgcac atctggccgg
     2641 tcggcactgg tttgactgaa ctttattccg aggccgcatg tgcccgccag caagtctgta
     2701 tcagctggtc atcgccttgc attcattggc caaggggcga ccactactgg tgtccatcaa
     2761 gacacttttc cagggcaggt cgatccacag caaaccattc ttccggtcct cgaagcgagg
     2821 gaggccggag gtggagtcgt agcgctgcaa agtgtagtga cttctctgcc aatcgatttc
     2881 gatcaggttg gcattgcgcg catcgcgctg aacttcaacc ttttgcccga gctcgcaacg
     2941 atagataccg ctggcgagct tgaaattcaa ctgctgctgg cggcctttga cgacttcgta
     3001 gcgggtagaa gatgtcgccg gctgcgaccc aaacaacgag caggcagtca gcgtgcctga
     3061 cagggtggtc agagcgagta tgttgatcag gtgattaagc gtcacgcgca gtgcttccgg
     3121 gtaggggcag gcaaaatagc atgacttgcg acggccgggc cggatagttc gcaatcgtga
     3181 tggaggtggc gacaagcggc ccgattggaa gatgtccggt ccgtgttggc gtcgctcatg
     3241 cgtttgattt gcctttctat gagccaacca tgcgcttgta gagcgccgat atttcgccga
     3301 cgatcccccg gcggaagagc aggacgcaga tcacgaagat ggtgcccatg atgacggtga
     3361 ccagcgagcc gaccttgtcg gcaagttcgt tctgcagact cacgatcacc gtagccccga
     3421 ctactgggcc cagcaccgtg cccatgccgc cgaggatggt catcaggacc acttctcccg
     3481 agctgtgcca gtgggcgtcg gtaagcgtcg cgaacttgaa caccagcatc ttggtcgcac
     3541 cggccagacc ggccagcccg gcggaaagga cgaaggcgag cagcttgtat ttcgctacat
     3601 cgtagccgag ggatatcgcc cgcggttcgt tctcgcggat cgccttcagg atctggccga
     3661 aaggcgaatg gatagtgcga tagatcaggc agaaaccgaa gacgaaaatc gccagcacaa
     3721 aatagtaaag attcaggtcg ttggccaaac tgatcagccc gaggagcttg cccctgggaa
     3781 cgccctggag gccgtcctct ccgccggtaa agggcgcctg cagaaagaag aaatacacca
     3841 tctgcgccag cgccagcgtg accatggcga aatagattcc ggtacgacgg atcgccaggc
     3901 tgccgaccac ccagccgagc agggcagatg cggcagcgcc agcgagaatt cccagttcgg
     3961 gtgggaaacc ccaccccttg atcgcgtaac cggcaacata ggcggcggtt ccgaagaagg
     4021 cggcatgacc aaatgagagc agcccggtat agccgagcag caggttgaag gcgcaggcaa
     4081 acagcgcaaa acagagcacc ttcatgacca ggaccgggta gacaaagaat ggcaagacga
     4141 tgcccagtac aagcagggcg aggtaagcga ttaccgttcg gttcatttct cttttccgaa
     4201 caatccggcc ggtcgtacca tcagcacgat cgccatgatg acgaagacca ccgtgttgga
     4261 cgccgccggg tagagcagat tggttatccc ttcgatcaca ccaaggccga ggccggtaat
     4321 gatcgagccc aggatagagc ccatgccgcc gatgaccacc acggcgaaga ccgtgatgat
     4381 cagatgcgag cccatcagcg ggctgacctg aatcaccggc gctgccagca cgccggcgaa
     4441 cccggccagc gctgcgccaa agccataggt cagcattacc attatcggca cgttgatgcc
     4501 gaaggcctgg gtcagctggg gattttcggt ggcggcgcgc agataggcgc ccaggcgggt
     4561 tttctcgatg ccgtaccacg tggcgaagca gaccatcagc gaagcgaaaa tgacccagcc
     4621 acgatagtag ggcagaaaca tgaagcccag gttgaagccg ccagcgagtt gagcggggat
     4681 ctggtacggt tgtcccgaga cgccataagc attgacgaac aagccctcaa tgaccagtgc
     4741 caggccaaag gtcagcagca ggccatagag atgatcgagt ttgtagagcc agcgcagcat
     4801 gccgcgttcg atcagaacgc cgaacacgcc gatggccaga ggagccagga tcagcgcagg
     4861 ccagtagccg attcccatgt attcgagacc catccaggcc aggaatgcgc ccatcatgta
     4921 gagggcgccg tgggcaaagt tgatgatgtt gagcatgccg aagataaccg ccaaaccgag
     4981 gctgagtacg gcgtagaacg aaccgttcac caggcccagc atcagctggc ccatcagcgc
     5041 ctggatcggt atgtcgaaga gtgcagtcat ggcgtttcat cacggcgaca cggcgaagta
     5101 cgccgtgtcg ccaagactgt tattttttca caagcgggca ttgtgatgcc gacagcggct
     5161 ggaacgcatc ttcgccggaa accacagcct tgatgtcgta ataatcccag gggtacttcg
     5221 actcggacgg cttcttcacc tgcatcagat acatgtcgtg gatcatgcga ccattctcac
     5281 ggatcttgcc gttcttggcg aagaagtcat tgatcggagt tgacttcatc ttcgccatga
     5341 ccgctgccgc ttcatccgtc ccggctgcca tgaccgcctt cagataggtg gtgaccgccg
     5401 agtagtctcc cgcctgcacc atggtcggat tgcgcttggt cttgtcgaaa aagcgtttgc
     5461 tgaacttgcg cgtttcgtca tccttgtccc agtagaagcc cgaagtcagc agcatgccct
     5521 gggtggtttg cagaccaagg gcatgaatgt cggtgatgaa catcaggagt ccggcgagtt
     5581 gctgattctt ggtgataccg aattcgctgg ctgccttgat cgaattgatg gtatcgccgc
     5641 cggcattggc cagaccgatg atcttggcct tcgatgcctg tgcttgcagc aggtaggacg
     5701 agaagtccga ggcattgaga gggtgccgta ccgagcccag aaccttgccg ccgttggcct
     5761 tgacaacgtc agaggtgtcc ttctccagcg agtgcccgaa ggcatagtcg gcagtgagga
     5821 agaaccaggt gtcgccgcca ttcttgacga tcgccttgcc ggctacgttg gccagcgcat
     5881 aagtgtcata ggcgtaatgc accgtattcg gcgtgcaatc ctgatcggtc agccgcgtcg
     5941 aaccggagcc gacgaatatc gcgattcgct tcttttcttg cgtcactttg ccaacggcca
     6001 gcgacacgcc ggaattgagc aagtcggtca ccatgtccac gccctcggtg tcgtaccatt
     6061 cgcgcacctt gttcgagccg acgtcggcct tgttctggtg gtcggcataa accatctcga
     6121 tggcgaactt cggtttgtac tgcgccttga agtcgtcgat cgccatctgc accgcagtaa
     6181 ccgcgccttg acctccaagg tcggagtaga cgccggacat gtcggtaagc acgccgatct
     6241 tgaccatgtt gcccgagata ttcatggcgg cgggcttggc ctctgccgag gcgaagttgg
     6301 cggccggaat cagtagcgct gcaagcgcga cgttaagcag tttgtatttc atggttttgt
     6361 ctcctgtggt tgttgtgcgc cggataatgc aatccgacag ttgaaacgcc ttagactcca
     6421 agatactcct gcaacaattc ctgcttggtt tcaatctcgt tttgagcaat ttcggcagcg
     6481 atctgtccat gctcgacaat gtacatgcga tccgccaacg gcgccgcaaa atggaaattc
     6541 tgttcgacca gaacaatcgt gaaccccttg cttttcaagt cgccgatcac ctgtccaagc
     6601 tgttgcacga tcactggtgc aagtccttcc gaaatctcgt cgagtagcaa cagttgcgca
     6661 ccgctgcgca ggatgcgcgc catggccagc atctgttgtt ccccgccgga caggcgtgtg
     6721 ccctggctat gacggcgctc ggcaagattc ggaaacattg cgtagatttc gtccagacgc
     6781 atgcctccgc tgccaacctt gggcggaagc aggagattct cttcgcaact cagactggcg
     6841 taaatgccgc gttcctccgg gcagtaacct acgcctagct gtgcgatgcg gtgcgttgcc
     6901 atgctgacgg tttcgcgtcc attgagcatg atcgaccccg ttcttttgcc ggtgaggccg
     6961 aggatggcgc gcagcgtggt ggtacggccg gcaccattcc tgccgagcag cgatacgcac
     7021 tcgccgcggt ttacccgcaa atccatgcca tgcaggatat gcgattcacc atagaaggca
     7081 tgcaggtcgc tgatgcgcaa atattcaacg gccgacgtca cgcctgactc ccggaagcct
     7141 cattctcggc cgtccccatg taggcctcga taacctgcgg atttttggcg acttcggcat
     7201 agggcccttc ggccagtacg gagccgcgcg caagaaccgt gatcgtgtcc gagatgccgg
     7261 caacgacctt catgttgtgt tcgaccatga ggatggtgcg atccgccgag accttcttga
     7321 tcagttgcat cacgcggtct acgtcttcgt ggcccatgcc ctgggtcggt tcgtcgagca
     7381 ggatcagttc gggctccagg gccagtgtcg tggcaatttc caaagcgcgc ttctggccat
     7441 agggcaactc gacggcaacc atccggttct gagcatccag gcccacttcg gccagcaatt
     7501 cgatggcgcg cgtgtcgagg cgtgacaggg ttttgtccga gcgccagaaa tagaatgaac
     7561 cgccgagctt ccgctgcaag gcgatgcgga cgttctcaag cacggtcaga tgcggaaata
     7621 ccgccgaaat ctggaaggaa cggacgatcc cgcgccgcgc gatctttgcc ggcgggtcgc
     7681 ccgtgatgtc gatgttgttg aatcggatgg cgccgcgggt gggctggaga aacttggtga
     7741 gcaggttgaa gcaggtggtc ttgcctgcgc cattcgggcc gatcatcgcg tgaatatgcc
     7801 cgcgcctgac tttcagactg acgttatcga cagcgacaaa tcccttgaat tccttgacca
     7861 gattatccgt ttcaagaatg tagtcgttca tagccctgga ttttgccctt cgtcttggac
     7921 gtcgccacgc gtgtcgcatg agcatccctc gaacgggatg catcggcgga ttatcagaca
     7981 ggcaattgct ttaggcaagc aagaatatta ctcattccga cgcgcgtgat ggaaggtgtt
     8041 tcaatcgacc agcctctgtt gcgcaacaga agaagcaggc cttccggcag gcaacttcat
     8101 gctggtcgga gccggtcgca ccctcgccgc cgggtgggcc atcgtcgcat gggcgggcat
     8161 cgtgctggtg ctcgcctcgg tcgcctcgct cgcggtcgtg acgattcgct
//
LOCUS       DAORPM010000098         8048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2203_length_8048_cov_7.133242, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000098 DAORPM010000000
VERSION     DAORPM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_8048_cov_7.133242"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1074
                     /locus_tag="PLD53_06285"
     CDS             277..1074
                     /locus_tag="PLD53_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRC60555.1"
                     /translation="MKKASPLMQNPQQAIVDITLIMLVAATAIVILAQYTDLDQIIAN
                     YYFDPERMIFPWDTTWFGRKLMHGYVKATIVWFGFLLIVVTVFDLVRPLRRFTPLRYL
                     TPLGRLKLRFLTLAAVLEPKLILSLKEASNMHCPWGVDLYGGKAPMLRLLDWVPEGWE
                     AGQCFPAGHASAGMWLSALAILWLPTHPRKALATFVAGLSVGLFMGWVQQMRGQHFLS
                     HTLATAWLSTALLLFMLAVFWRPMQRALIRDSDANSNENTPALVWQQ"
     gene            1071..2774
                     /locus_tag="PLD53_06290"
     CDS             1071..2774
                     /locus_tag="PLD53_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HRC60556.1"
                     /translation="MTTTAIDMGLKPARPFLAQLAKRPSLGVETLILLVCLWFTATSN
                     SLFWQATLDSRAGSWASIAFHGGALALLLTAIHFLLLAIFIPRRAAKPLLATLILATS
                     FAAYYMGKYHVYLDPDMLRNIMRTDYREASELFTWGMIPAVAAYALPPLLLLARTDIR
                     PRPLMRAVGIRLLCLFTAVAVTGASGFAIFKGLSSLMREKKEVRYLITPANYLYSGVR
                     AFANDSAATAREKIPVGADATLSAAWAQHKKPVLMIVVIGETARAANWGLSGYARQTT
                     PELARLDVINFSDVTACGTNTEVSVPCMLSPYGRNSYDETKIRSSESLLHVLKHAGLR
                     VSWIDNQSGCKGTCDGLESLHPDVKASPADCNSDGCMDGAMLTGVRQLAEGSRESQVI
                     FLHTLGNHGPAYSKRYPESFKRFLPACESSDLGKCTQQEIVNAYDNALLYTDHVLAQA
                     VEFLKTQQSSYDTALLYVSDHGESLGENGLFLHGIPYAIAPEIQKKVPMVAWFSDGYA
                     KRFSLDTQCLRQRAHSAVTHDHLFHTTLALLDVRTSALDDKYDFSAGCKANYRQQIAA
                     K"
     gene            3001..3663
                     /locus_tag="PLD53_06295"
     CDS             3001..3663
                     /locus_tag="PLD53_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRC60557.1"
                     /translation="MRVLLVEDDTLLGDGIRAGLKLANYAVDWVQDGEAARLAILSHD
                     YDACVLDLGLPKKDGLTVLRDLRSRGDTLPVLILTARDTNADKIAGLDAGADDYLAKP
                     FDLGELQARMRALIRRASGAATPVLAHAGVTLDPAGKRVTKDGQPLQLSAREYALLFD
                     LMTNRNQMRTRSQLEESLYAWGEEKESNTVEVYVHHLRKKLGSDFIRTVRGLGYQLGD
                     AT"
     gene            3660..5078
                     /locus_tag="PLD53_06300"
     CDS             3660..5078
                     /locus_tag="PLD53_06300"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC60558.1"
                     /translation="MSVAGLAPLNARSLRWRLLRWVTLTTIVIWGLAAGLSYHQARAE
                     VQELMDGQMAKMARLLLAQIHEDTSYLKDLPENMNSLRGTKSRRSELALEFQIGYPDG
                     TVLVNSAMAPETPLTRPLGYANVEHAGHPWRSLILETADGRYRAQVAHSFHTRDKEAL
                     EIASKTVLPLGLLLPLMVALLYFSIQRCLKPLDDLAEDVASRSPDNLTTLQPETTLLE
                     TQPLVRSLNHLLTRLATTLDNERRFTADAAHELRTPLAALKIQAQVAMATKNPEERDH
                     AMAQVLAGVDRTTRLVEQLLRLARLDPIVQLSDTQPIDLLELARGALESAHSAAATRR
                     QRLHLTAPMDAPRITGDPDLLGAALRNLLDNAVRYGPEQSTVTLTIDNDPGGLRLEVS
                     DEGPGTPEEDLSHLTNRFFRSSEAGSEGSGLGLSIVQRIAELHGARLELENRQGGGFV
                     ARLRWPIDGKTGGLATTSGWKI"
     gene            5193..6920
                     /gene="cptA"
                     /locus_tag="PLD53_06305"
     CDS             5193..6920
                     /gene="cptA"
                     /locus_tag="PLD53_06305"
                     /inference="COORDINATES: protein motif:HMM:NF007933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HRC60559.1"
                     /translation="MTHAAQAGKLSNKTDWHGLLLVYFFFWYFSGVHHLLLQVIDAIS
                     FASFRLATVASTLWLIPVLLAPKYTRQIAALIGIVLWVFSLVSLGYFFIYQNEFSQSV
                     LFIIFESNPAEASEYVAQYFVWWMIPAFVIYSLAAWLIWRKLRPVAISPKRAWFVIAI
                     VVTTLFAYPQFKNLRGGVISSAVAADAIIKRIEPAVPWQILVGYSEYRQQLAAVEELV
                     AKNHEIPPLKNLIDRNGSTPTTLVLVIGESTNRQHMSLYGYHRQTTPRLDALSKELTV
                     FDNVVASRPYTIETLQQALTFADQENPDLYLSTPSLMNIMKQAGYRTYWITNQQTMTQ
                     RNTMLTNFSKQTDEQVYLNNTRVQNSRAYDGAVLDPFGKILADGVEKRFIIVHLLGTH
                     MRYEFRYPPEDEYFKGRDGLPEWADEEQTQFINEYDNAVRYNDFVVGSLIEQLRSAQL
                     KSLLLYFADHGEDVYDTPPHKFIGRDEAKPTPAMYTVPFVLWQSESWRTAHPRQFAGI
                     TRRPYQTSHLIHTWAELVGLSFDGLDPTKSVISTKFRERPLLVGDPSAPKGLIDLRPM
                     LKPSEKPVK"
     gene            7144..>8048
                     /locus_tag="PLD53_06310"
     CDS             7144..>8048
                     /locus_tag="PLD53_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60560.1"
                     /translation="MHLAHLMQFHEESRVIAWWQANLGGMLRWFTPFRRRTILAVAGV
                     WVCIKESRHNFTPADVPVSADRLGPVLLVLALLGMSWLFYRAATGFSRLPRFVRTHPQ
                     LSLHSVYWGLLVVLWTSAPDSGLWRQVLLGVAGTFPFLLWRFGYLLLSGQHGRMTGTR
                     FSDHFLTIWPIYGGSNTPYGKGFGYLSRFEAKSEEELARSQLAGIKLYILSNIWLASM
                     KVFEGVIYGPGNELTRMLGGYTLGIPKLSYLVAMESQETAVWISWISIYCELVYQVLR
                     HAVHGHVVIAILRIFGFNVFRNTYKP"
BASE COUNT         1692 a         2500 c         2200 g         1656 t
ORIGIN      
        1 ctgtttacca gcaggaaagc ttggcccacg cattccatcg gccatgcgtg aacgtggtga
       61 ggcatggggc caccttcatg tcagaaacac agcgaacggc gttggcaccg gccgcactct
      121 agcggcgcaa ttttaaacca gtcttaaaac cacgctgtcg taaagaggca cacaaatcga
      181 cagcaagggc attctgcacg cgcatcttaa gggtcattta aggttccgac gcaacactgc
      241 ggccactcaa gtcataacgt cgatcacgag gcccccatga aaaaagcatc gcccctgatg
      301 cagaacccgc aacaggcaat tgtagacatc accctgatca tgctcgttgc cgccaccgcc
      361 atcgtcatcc tggcgcaata cacggatctc gatcagatca ttgccaatta ttatttcgac
      421 cccgaacgca tgattttccc ttgggacact acctggttcg ggaggaagct gatgcacggc
      481 tacgtcaaag ccaccatcgt ctggtttggg tttctgctca ttgttgtcac cgtgttcgat
      541 cttgtgcgcc ccctgcgccg cttcactccc ctgcgctacc tcactcccct cggccgcctt
      601 aaactgcgct tcctgacgct ggctgccgtc cttgaaccga aactgatcct ctcactgaag
      661 gaagcatcca acatgcactg cccctggggt gtagacctct atggtggcaa agcgccgatg
      721 ctgaggttac tcgactgggt gccggagggg tgggaggcag ggcaatgctt ccctgccggc
      781 catgccagtg ccggcatgtg gctctccgca ctggccattc tttggctacc cactcacccg
      841 cgcaaggcac tggcaacctt tgtcgccgga ctcagtgtcg gcctgttcat gggttgggta
      901 caacagatgc gtggccagca tttcctgtcg cacaccctgg ctacggcctg gctcagtacc
      961 gcgctgttgt tgttcatgct ggccgtattc tggcgcccga tgcagcgcgc tctgatacgg
     1021 gacagcgatg ccaattccaa tgaaaacacg cccgctctgg tttggcagca atgacaacaa
     1081 ccgccatcga tatgggatta aaaccggcaa gaccctttct ggcgcaactt gcaaagcggc
     1141 cgtcgctcgg tgttgaaaca ctgatcctgc tcgtctgcct gtggttcacc gccaccagca
     1201 attcgctgtt ctggcaagcc acgctggata gccgcgcggg ctcctgggcc agtattgcct
     1261 tccatggtgg cgccctggca ctgctgctga ctgccatcca ctttctgctg ctggcaatct
     1321 ttatccctcg gcgggccgcc aagccactgt tggcgacgct gatcctggcg acatcctttg
     1381 ctgcctacta catgggcaaa taccatgtct atctcgaccc ggacatgctc cgcaacataa
     1441 tgcgtacgga ttatcgcgaa gcgtcagagc tgttcacatg gggaatgatt ccggcggtag
     1501 ccgcctatgc tctgccacca ctcctgcttc tggcccgcac cgacatccgt ccacggccgt
     1561 tgatgcgcgc tgtcggcatc cgcctccttt gcttgttcac tgcagtggcg gttaccggcg
     1621 catccgggtt tgccatcttc aagggcttgt catcgctgat gcgcgagaag aaggaagtgc
     1681 gctatctgat tacaccagcc aactacctct actccggcgt gcgtgctttt gccaatgact
     1741 cagcggccac cgcccgtgaa aaaatcccgg tcggtgccga tgccaccctc tcggccgcct
     1801 gggcacagca caaaaagccg gtgctgatga tcgttgtcat cggcgaaacg gcgcgtgccg
     1861 ccaactgggg actatccggc tatgcgcggc aaaccacccc agaactggcc aggctcgatg
     1921 tcatcaattt cagcgacgtc accgcctgcg gcaccaacac cgaagtctcg gtcccctgca
     1981 tgctgtcgcc ctacggacgg aacagctacg acgaaacgaa gatccgcagc agcgaatcgc
     2041 tgctccatgt gctgaaacac gccggcctac gcgtctcctg gatcgacaac cagtccggtt
     2101 gcaagggcac ttgcgacggc ctggaaagcc tgcacccgga tgtcaaggcg tcgcccgccg
     2161 actgcaatag cgacggttgc atggacggtg ccatgctgac cggcgtccgc caattggccg
     2221 agggcagtcg cgaaagccag gtgatcttcc tgcacacgct cggcaatcac ggcccggcct
     2281 actcgaagcg ctatcccgag tcattcaaaa gattcctgcc ggcatgcgag tcgtcggatc
     2341 tcggcaaatg cacgcagcag gaaatcgtga atgcctacga caatgccctg ctctacaccg
     2401 accatgtcct cgctcaagcc gtcgaattcc tcaagaccca gcagtccagc tacgacacgg
     2461 ccctcctcta cgtatcggat catggcgaat cgctcggcga aaacggcctc ttcctgcacg
     2521 gcattcccta cgctatcgcc ccggagatac agaagaaagt cccgatggtg gcatggttct
     2581 ccgacggcta cgcgaagcgc ttctcgctcg acacgcaatg ccttcgccag cgcgcacaca
     2641 gcgccgtcac gcatgaccac ctctttcaca cgacactggc gctgcttgat gttcgtacaa
     2701 gcgcgctcga cgacaagtac gatttcagcg ccggttgcaa agccaactac aggcaacaga
     2761 tcgcggcaaa atgattgcgc ttgctttagc caatcagtca gcggcaaacc cccgagtggg
     2821 tacgcagcag gcttcggcgt ttgccatagg agttgcccag cgacggttct tatgcgcccc
     2881 ggcagagcaa gaggattgac cctcgttgaa attccagtaa gtatccaggc atctcgccga
     2941 aaacgccggc cacccctgta tttcggcata ctcccctact ccggacccaa ggacaaagca
     3001 atgcgtgttc tgctcgtgga agatgacacc ctgctcggcg acggcatccg tgccggactg
     3061 aaactggcga actatgccgt ggactgggtt caggacggcg aagcagcccg attggccatt
     3121 ctcagccacg actacgacgc ctgcgtactc gatcttggcc tgccgaagaa agatggactc
     3181 accgtattga gagatttgcg cagccggggc gataccctgc ccgtgctgat cctgacggct
     3241 cgcgacacca acgccgacaa gatcgccgga ctcgatgcag gtgcggacga ttacttggca
     3301 aaacccttcg acctgggcga gttgcaggcg cggatgcgtg cactcatccg ccgcgcctcc
     3361 ggcgccgcca ccccggtgct tgctcatgcc ggagttacgc tcgacccggc gggcaaacgt
     3421 gtcaccaaag acgggcagcc cctgcagctg tcagcccggg agtatgcgct gctgttcgac
     3481 ctgatgacca acaggaacca gatgcgtact cgcagtcaac tcgaagaaag cctttatgcc
     3541 tggggcgagg aaaaggaaag caacacggtg gaggtctatg tccatcacct gcgcaagaaa
     3601 ctgggtagcg atttcattcg cactgtgcgt ggtctaggtt atcaactggg agatgctacg
     3661 tgagcgtcgc tggattggcg ccactcaacg cccgctccct gcgctggcga ctgctgcgct
     3721 gggtgaccct gaccaccatc gtgatctggg gtctggctgc cgggctgagc taccatcagg
     3781 cacgtgccga agtgcaggag ctgatggacg ggcagatggc caagatggca cgcctgctgc
     3841 tcgcacagat acatgaagac accagctatc tgaaagattt gccggaaaac atgaacagcc
     3901 tgcgcggcac caaatcgcgg cgcagcgaac tggcgctgga gttccagatc ggttatcccg
     3961 acggcacagt gctcgtgaac tcagccatgg cgccggaaac gccgttgacc cggccactcg
     4021 gttacgccaa cgtcgaacac gccggccacc cctggcgcag cctgatcctg gaaacagccg
     4081 atggccgcta ccgggcgcag gttgcacatt ctttccatac ccgtgacaag gaagcgctgg
     4141 aaatcgcctc caagaccgtg ctgccgctag ggctgctgct gccactgatg gttgcgctgc
     4201 tctatttttc gatccagcgc tgcctgaaac cgcttgatga tctggccgag gatgtagctt
     4261 cgcgctcgcc ggataatctc acgacgctcc agccggaaac aaccctgctc gaaacccagc
     4321 ctctggtcag gtcgctgaac cacctgctga cacggctggc gaccacactc gacaacgaac
     4381 gacgcttcac agccgatgcc gcgcacgaac tgcgcacccc actggccgca ctgaaaatac
     4441 aggcacaggt ggcgatggcc acaaaaaacc ccgaggaacg cgatcacgca atggctcaag
     4501 tgctggccgg agtcgaccgc acgacgcggc tggtcgagca attgcttcgc ctggcgcggc
     4561 tagacccgat cgttcagcta tccgacacac aaccaatcga cctgcttgaa cttgcccgtg
     4621 gggcgctcga aagtgctcac agcgcggcag caacgcggcg acaacgcctt catctcactg
     4681 ctccgatgga cgctccccga atcaccggcg acccggacct gctcggtgcc gcgctgcgca
     4741 acctgcttga caatgcggtg cgctacgggc ctgagcagag tactgtgacc ctcaccatcg
     4801 acaacgaccc tggcgggtta cggcttgagg tcagtgatga agggccgggc actccggaag
     4861 aggatctatc acacctaacc aatcgcttct ttcgcagcag cgaagccggc agcgaaggaa
     4921 gcggcctagg cctctccatc gtgcagcgca ttgccgagtt acatggcgct cggctggagc
     4981 tcgagaacag gcagggcggg ggttttgttg cccgcctgcg ctggccaatc gatggcaaga
     5041 ctggcggctt ggctaccacc tctggctgga agatatagag actcatcggt attattcaag
     5101 tatgttctcg gacgggtgag cgatcatttc gcgtcagttg aaaagcgctg ccctgacact
     5161 ttccggcggc accattttgc aaggaaaccg gcatgaccca cgctgcccag gcaggcaagc
     5221 tctcgaacaa gaccgactgg cacggattac tgctggtcta tttcttcttc tggtatttct
     5281 ctggcgtgca ccacctgctg ctacaggtaa ttgatgccat ttccttcgcg tcgttcaggc
     5341 tggcaacggt ggccagcacg ctctggctga tcccggtact gctcgccccg aagtacacac
     5401 gccagatagc cgcgctaatc gggatcgtgc tctgggtctt ttcactggtc agcctgggct
     5461 acttcttcat ctatcaaaac gaattttcgc agagcgtcct gttcatcatc tttgaatcga
     5521 acccggctga ggcgagcgag tatgtcgcgc agtattttgt ctggtggatg atccccgcat
     5581 tcgtcatcta ctcgctggct gcgtggttga tctggcgaaa attgcgaccg gtggccattt
     5641 ccccgaaacg cgcctggttt gtaattgcaa tagtggtgac gacactcttt gcatacccgc
     5701 agttcaagaa tttgcgcggc ggcgtcattt cttcggcggt cgcagcggac gccatcatca
     5761 aacgcattga gccggccgta ccttggcaga tcttggtcgg ctactcggag tatcgccagc
     5821 aattggcggc tgtcgaggag ctggtggcaa agaaccatga gatcccgcca ctgaagaatc
     5881 tgatcgaccg taacggaagc accccgacca cgctggttct ggtgattggt gaatcgacca
     5941 accgtcagca catgagtctc tacggctatc accgccagac cacacctcgc ctcgatgccc
     6001 tgagcaagga gctgaccgtc ttcgacaatg tggtcgcctc acgcccctac accatcgaga
     6061 cactccagca ggcgttgact tttgccgatc aggaaaaccc cgatctctat ctgagcacac
     6121 catcgctgat gaacatcatg aagcaggcgg gctatcgcac ttactggatc accaaccagc
     6181 agacgatgac tcagcgcaac acgatgttga ccaatttttc gaagcagacc gatgaacagg
     6241 tttatctgaa caacacccgt gtacagaatt cccgcgccta cgacggtgca gtgctagacc
     6301 ctttcgggaa gatacttgct gacggcgtgg agaagcgttt catcatcgta cacttgctgg
     6361 gcacgcacat gcgctacgag ttccgctacc cgccggagga cgagtatttc aagggtcgcg
     6421 acggcttgcc tgagtgggcc gatgaggagc aaacccaatt catcaacgaa tacgacaatg
     6481 ctgtgcggta taacgacttt gtcgtcggca gtctgatcga acaattgcgc agcgcacagc
     6541 taaaaagcct gttgctctat ttcgccgatc acggcgagga tgtgtacgat acgccaccgc
     6601 acaaattcat tggccgcgac gaagccaagc cgacaccggc aatgtacacc gtgcccttcg
     6661 ttctctggca atccgaatca tggcgcacag cccatccacg gcagtttgcc ggaatcaccc
     6721 gccgaccgta ccagacgtca cacctgattc acacctgggc ggaactggtc gggttatcgt
     6781 tcgacggtct ggacccgacc aagagtgtga tcagcaccaa atttcgagag cgcccgttgc
     6841 tagttggcga cccatccgcc cccaaagggc tgatcgacct gcgcccgatg ctcaagccat
     6901 ccgaaaagcc cgtcaagtag cagccggttg ccgcggcatt gtatgtgcgc acccacggca
     6961 gtagtgccgg agagctcaaa caggctgcca gtgtccttca gcgaagctgg ctaggggctc
     7021 ccggatgaat ccgcggaagc agaatttgcg ccaagatcga ccacacgcca taatgccgcc
     7081 tgatttgacg cccccatacc ctgcgtgcaa cgcagttgtt ggcactttcc caccggtatt
     7141 cccatgcatt tagcccactt gatgcaattc catgaagaat ccagggtcat tgcctggtgg
     7201 caggcgaatc ttggtggaat gctgagatgg ttcacgccat tcaggcgaag gacgatacta
     7261 gcggtggctg gcgtctgggt atgtatcaag gaatcccggc acaatttcac gcccgccgat
     7321 gtgccggttt cggctgaccg cctgggtccg gttctcttgg tgcttgccct gctcggaatg
     7381 agctggctgt tctaccgggc ggccacggga ttctcccgct tgccacggtt tgtcagaacg
     7441 catcctcagt tgtccttgca ctcggtttac tggggattgc tcgtggtact ctggaccagc
     7501 gcaccggaca gcgggctttg gcggcaagtt ctgctcggcg tcgcggggac gtttccgttc
     7561 ctgctctggc gattcggata ccttctgctt tcagggcagc acggccgaat gaccggaaca
     7621 cgattctccg accatttcct cacaatctgg ccgatctacg gtggatccaa tacgccgtat
     7681 ggcaaaggct tcggatattt gtcccgattc gaagcaaaaa gcgaggagga acttgcccgt
     7741 tcgcaactgg caggaatcaa gctttatatc ctgtccaata tatggcttgc ctcaatgaag
     7801 gtattcgaag gggtcattta tggccctgga aatgagctga ccagaatgct cggcggatac
     7861 accctgggaa tccccaagct ctcttatctg gttgcgatgg agagtcagga aaccgctgtc
     7921 tggatttcct ggataagcat ctactgcgag cttgtttatc aggtgcttcg ccatgcagtt
     7981 catgggcacg tagtcattgc catcctgcgg atttttggtt tcaacgtatt ccgcaatacc
     8041 tacaagcc
//
LOCUS       DAORPM010000099         7998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2224_length_7998_cov_10.807755, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000099 DAORPM010000000
VERSION     DAORPM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2224_length_7998_cov_10.807755"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2631
                     /locus_tag="PLD53_06315"
     CDS             <1..2631
                     /locus_tag="PLD53_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRC60561.1"
                     /translation="SLYVGYGNGDFFLVRRIWNGDERKHYDAPEQTAYVVQSIDHERT
                     APRGRFIFLDSSLATLRSDERPDYATSYDPRTRDWYKTAMTTSGQIKTPPYLFYTNKQ
                     VGTTLANRAGATSAVVGADILLATLDQTMAKQKVTPGTHTVLVNRTGQILAHEDNAKV
                     IRLPPTADGRPVLATLSDFGAPVLAQLSPLIQGANDEMMQTRTLEVDGETWYVTVSPL
                     KLEGAAPLFLISAIPEVELMAAAHTLMRQLFVAMALVIALAIPITWSLARAISGPLRK
                     LAGEADAIRHFEFSKPVTVDSPVLEVSELALTMDSMKRTIRRFLDISMAVAAENNFDR
                     LLPRLLTETISAADADAGVLYLTDNDVLLPATGLLSDGSELPPSAPRPDSITGSPLLH
                     AAIQTRTARSARIAEADIDALGLVDSVKASGASHAIAVPLLNRQQHLVGAMVLLRNGE
                     TDDARLSFIGALSGSAAVSLEAKALIRAQKLLFEALIQLIAGAIDAKSAYTGGHCARV
                     PELTKMLARAACDQTSGLYKDFSLNEDEWEAVHVAAWLHDCGKVTTPEYVVDKATKLE
                     TLYDRVHEIRMRFEVLKRDAEIACLKAIAAGKSEASAREKLSAELARLDDDFAFVASC
                     NEGGEFMAPDKQERLRSIAERTWLRTLDDRIGISHEEKERKERSPAPDLPVLEPLLAD
                     KPEHRLERQAQDRMPENNRWGFKMPVPELLYNKGELYNLSVARGTLSEEERYKINEHI
                     VQTLIMLSELPFPKHLREVPEIAAGHHEKMDGTGYPKRLHREEMSPLARMMAIADIFE
                     ALTAVDRPYKKGKTLSEAIKIMSFMKKDQHIDPDLFDLFLRSGIYRDYAARFMRPEQI
                     DEVDISAYVSPPAEPASPLA"
     gene            2670..3431
                     /locus_tag="PLD53_06320"
     CDS             2670..3431
                     /locus_tag="PLD53_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRC60562.1"
                     /translation="MLSDPFNLILLLTAAFAAGALNAVAGGGSFLTLPALVFTGMPTV
                     TANATGTVALLPGYASGAWGFREDLEAPPGLSLRALTALSLIGGATGATLLLLTDDRT
                     FSRIVPWLLLAATILFAVGPMLLSKLKNHQTGHASAKKSAAGLLAVSIYGGYFNGGLG
                     ILLLALFGLLGQTSLNAMNGIKNVVSALLTAIAVGIYAWGGVVAWPQALVMMIAATAG
                     GYFGARIARRIPAPVLRASIVATGLIMTALFFLRG"
     gene            3705..4127
                     /locus_tag="PLD53_06325"
     CDS             3705..4127
                     /locus_tag="PLD53_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRC60563.1"
                     /translation="MGIQFNPEQGTILICDFKGFLQPEMIKRRPVVVVSPRLRKRHGL
                     CTVVPLSTTPPKEVAAYHFKLHVVPVLPAPYNAAFHWVKADMVYTVSFDRLFLPFGTK
                     DGSGKRIYDTRVIDKADLLKIQHCMLHGLGLSALTDYL"
     gene            complement(4349..4435)
                     /locus_tag="PLD53_06330"
     tRNA            complement(4349..4435)
                     /locus_tag="PLD53_06330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4399..4401),aa:Leu,seq:caa)
     gene            4523..5602
                     /gene="queA"
                     /locus_tag="PLD53_06335"
     CDS             4523..5602
                     /gene="queA"
                     /locus_tag="PLD53_06335"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRC60564.1"
                     /translation="MLTLDDFDFSLPPELIAQHPTPERSASRLLHLAGDAQHDRQFAE
                     LPQLVEPGDLLVFNDTKVIRARLLGQKESGGRIEVMIERIVDRDRAVAQIRASKSPKS
                     GSRIVLENAIVLTVTGRTGEQSEFFALELVGDGDLFELVEQYGHLPLPPYITHAAGPE
                     DETRYQTVFARHAGAVAAPTAGLHFDHATLAQLGERGAELAWLTLHVGAGTFQPVRVN
                     NLAEHHMHTERFEIPQATVDAIARTRARGGRVIAVGTTSLRALEAAAQNGKQGGELQA
                     GAAETDIFITPGYRFRVVDRLITNFHLPKSTLLMLVSAFAGTDIIRAAYRHAIAERYR
                     FFSYGDAMLLEKLDENSHENANNAI"
     assembly_gap    5702..5801
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5802..6302
                     /locus_tag="PLD53_06340"
     CDS             <5802..6302
                     /locus_tag="PLD53_06340"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRC60565.1"
                     /translation="DGFAIGGLSVGEPKDEMARILAHTAPRLPQNKPRYLMGVGTPED
                     LVAAVLTGIDMFDCVMPTRNARNGHLFTRYGDIKIKNAQYKNDPRPLDEGCECYTCRN
                     FSRAYLHHLHRVGEILGAQLNTLHNLHYYQTLMGELRKAIGTGELARTVARFHAGRSD
                     RGGVIQ"
     gene            6353..6679
                     /gene="yajC"
                     /locus_tag="PLD53_06345"
     CDS             6353..6679
                     /gene="yajC"
                     /locus_tag="PLD53_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRC60566.1"
                     /translation="MLISNAYAQTAAAAGPLDNVMQFLPIILMFGVLYFLMIRPQMKK
                     AKEHKALLDALGKGDEVVTQGGIAGRITKVGEDFVTMAISDNVEIQMQKPAITLVLPK
                     GTLKSL"
     gene            6758..>7998
                     /locus_tag="PLD53_06350"
     CDS             6758..>7998
                     /locus_tag="PLD53_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HRC60567.1"
                     /translation="MNRYPLWKYIIVGFALVFGFIYTLPNFFGESPAVQVSSAKATIK
                     VDIKTLERVESTLKEASVENFGIFLDTNGVKVRLKDTDTQLKARDLLEKQFNPDPTDP
                     QYVVALNLLSSSPQWLTRMGALPMYLGLDLRGGVHFLLQVDMKGALTKRLDSLSADLR
                     SQLRDKTIRHGGINREGERLVIRFRDEETRNKGRLAVEDSQPDLLLADQGEGTDLKLV
                     ATLSPAAMKRIQEFAIKQNMGTLHNRINELGVAEPVIAQQGADRIVVQLPGVQDTAKA
                     KDILGRTATLEIRMVDDTPGALEAAMAGQVPFGTELYVERGGRPLLVKKQVVLTGDRL
                     TDAQPGFDSQTQEAAVHLNLDSAGSRIFRDITRENVGKRMAILLIEKGKGEVVTAPVI
                     RTEIGGGRVQISGSMSTMEAN"
BASE COUNT         1684 a         2451 c         2244 g         1519 t
ORIGIN      
        1 tccctgtatg tcggatacgg caatggcgac ttctttctgg tccgacggat ctggaatggc
       61 gacgagcgca agcactacga cgcgccggag caaaccgcct atgtcgtgca aagcatcgat
      121 catgaacgga ctgcgccgcg cggccgcttc atcttcctgg attcgtccct ggccaccctg
      181 cgcagcgacg agcgcccgga ttatgcgacc agctacgatc cacggacgcg ggactggtac
      241 aagacggcaa tgacgacctc cgggcagatc aagaccccgc cctatctctt ttacaccaac
      301 aagcaggtcg gaacgacgct cgccaaccgg gccggtgcga cgagcgccgt agtcggcgcg
      361 gacatccttc tggcaacgct cgaccagacc atggcgaaac aaaaagtgac gccgggaacg
      421 cacacggtac tggtcaaccg cacgggccag atcctggctc atgaggacaa tgcaaaggtg
      481 atccgcctgc cgcccactgc cgatggccgg ccggttctgg caacgctctc cgattttggc
      541 gctcccgtcc ttgcacaact gtctccgctg attcaaggcg cgaatgacga aatgatgcaa
      601 acccggacgc tggaggtcga cggcgagaca tggtacgtga cggtcagccc gctcaagctg
      661 gaaggcgccg ccccgctctt cctgatcagc gcgattcccg aagtcgaatt gatggccgcc
      721 gcccatacgc tgatgcgcca gttattcgtt gccatggcac tcgtcattgc tctggcgata
      781 ccgatcacct ggagcctggc gcgcgccata tccgggccct tgcgaaaatt ggcgggcgaa
      841 gccgacgcga tccgccattt tgaattctcg aagccggtca ccgtcgactc accggttctt
      901 gaagtcagcg aactggcgct gaccatggac agcatgaaac gcacgattcg ccgattcctg
      961 gacatcagca tggccgtcgc ggcggaaaac aatttcgacc gactgttgcc gcgactcctg
     1021 accgaaacca tttctgccgc cgatgccgat gcgggggtgc tctacctgac cgacaacgat
     1081 gttttattgc ccgcgacggg actcctgtcg gatggaagcg aattgccgcc cagcgcgccg
     1141 cgacccgatt cgatcaccgg cagcccgctg ttgcacgccg ccattcagac ccgaacggcc
     1201 cggtcagcgc gtatcgctga agcggacatc gacgccctgg gtttggtcga ttcggtgaaa
     1261 gccagcggcg caagccacgc cattgcggtg ccactactca atcgccagca acatctggtc
     1321 ggcgccatgg tgctgttgcg caacggtgaa acggatgatg cacgactcag tttcatcggc
     1381 gccctctccg gttccgccgc cgtctcgctg gaggccaagg cgctgattcg tgcccagaag
     1441 ctgctttttg aagccctgat ccagttgatc gccggcgcca tcgacgccaa gagtgcctac
     1501 accggcggac actgcgcccg cgttccggag ctcaccaaga tgctggcgcg cgccgcctgc
     1561 gaccagacaa gcggcctgta caaggatttc agtctgaacg aggacgagtg ggaagcggtc
     1621 cacgtcgccg catggctgca cgattgcggc aaggtgacga ccccggaata cgttgtcgac
     1681 aaggcgacca aactcgaaac actttacgat cgcgttcatg aaattcgcat gcgctttgaa
     1741 gtgctcaaac gtgacgccga aatcgcctgc ctcaaggcaa ttgccgccgg caaatctgaa
     1801 gcgtcggccc gcgaaaagct ttcagccgaa ctggcgcgac tcgacgacga cttcgccttt
     1861 gtcgcaagct gcaacgaggg cggcgagttc atggcccccg acaagcaaga gcgtctcaga
     1921 tcaatcgctg aacgcacatg gctgcgcacc ctcgatgacc ggatcggcat ttcccatgag
     1981 gagaaggaac gcaaggaacg ctccccggcg cctgatcttc cggttctgga gccgctactg
     2041 gccgacaagc ccgaacatcg cctggaacgg caagcgcagg acaggatgcc ggaaaacaac
     2101 aggtggggat tcaaaatgcc cgttcccgag ctgctctaca acaagggcga gctgtataac
     2161 ctcagcgtcg cccgcggcac actgtccgag gaggagcgct acaagatcaa cgagcatatc
     2221 gtccagacct tgatcatgct ctccgaactt cccttcccca aacacctgcg agaggttccg
     2281 gaaattgccg caggccacca cgaaaagatg gacggcaccg gctatccaaa acgactgcac
     2341 cgcgaggaaa tgagcccgct ggcgcgcatg atggcgattg cggacatctt cgaggcgctg
     2401 actgctgttg accgccctta caagaaggga aagacgctgt cggaagcgat aaagatcatg
     2461 tcgttcatga agaaggatca gcacatcgac cccgacctgt tcgatctgtt cctgcgctcc
     2521 ggtatctatc gcgactatgc cgcgcgtttc atgcgacccg aacagatcga cgaggtggac
     2581 atttctgcct acgtcagccc acccgccgaa cctgcgagcc ctttggcata agttggccca
     2641 caactcagat cttccgacat tcgcacccaa tgctgagcga ccctttcaac ctcatcctgt
     2701 tgctcaccgc cgccttcgct gccggcgcat tgaatgcggt cgccggcggc ggcagttttc
     2761 tgacgctccc ggcgctggtc tttaccggca tgccaacggt tacggcgaat gccaccggca
     2821 ccgtggccct gttgcccggc tatgcgtccg gagcctgggg gtttcgtgaa gacctggaag
     2881 caccgcccgg attgtcgcta cgcgcactga ccgccctgag tctgatcggc ggcgcgactg
     2941 gcgccaccct gctgctgctg accgacgacc gcaccttcag taggatcgtg ccctggctgt
     3001 tgctcgcggc aacgatactc ttcgccgtcg gcccgatgct gctgagcaaa ctgaagaatc
     3061 atcaaaccgg ccacgcctcc gccaagaaat cggctgcagg cttgctggcc gtatccatct
     3121 atggcggcta cttcaatggt ggactcggca tcctgctgct ggcgctgttc ggcctgctcg
     3181 gccaaaccag tctgaacgcg atgaatggca tcaagaacgt cgtttcggca ttgctgaccg
     3241 cgattgccgt cgggatttac gcctggggcg gtgtcgttgc ctggccgcaa gcattggtga
     3301 tgatgattgc agccactgcg ggaggttatt tcggtgcgcg gattgcgcgg cggatacccg
     3361 cgcctgtgtt gcgtgccagt atcgtcgcta ccgggttgat catgacagcg ctgttctttc
     3421 ttcgcggttg agcatggata cttctggatg cgagtcgcgg cacgcgcttg acgtgcttta
     3481 tttttcgtat tccaaaacaa cctgcgggct taaagaatcg ttacggatta ttatgagcta
     3541 ctgatggtgc gggaggggga atcgaatagt ttccgctatc cattgcagct acagaagtcg
     3601 agttttgaaa aagccaaaat atacccccaa atgcaccccc aggagcaggt ggtacgggag
     3661 gggttcaaga ttgatcacag cctgctgaag aacaagggca agtaatggga attcaattca
     3721 accccgagca agggacaatc ctgatctgtg acttcaaagg gttcttacag ccggaaatga
     3781 tcaagcgtcg accggttgtt gttgtatctc cacgactgcg taagcgtcac ggtctctgta
     3841 cagttgtgcc gttgagcacc acgccaccga aagaggttgc tgcctatcac ttcaaactgc
     3901 acgtagttcc tgtgcttcca gcaccctaca acgcggcttt tcactgggtt aaggccgata
     3961 tggtctacac cgtatcgttc gacaggctgt tccttccatt cgggaccaag gatggaagcg
     4021 ggaaacgcat ttacgataca cgcgtgatcg acaaagccga cttgctaaaa atccagcact
     4081 gcatgctgca tggccttggc ctgtccgcct tgactgatta cctctaggta acctatagtc
     4141 tgcctgtacc cgctctgttt cggcatcggg cacaagtcct cttcggaggc cgctacgcac
     4201 cggagattgc aatccagcgt agtgtctggg ccctgccgta aggtggggcc tttgcttttg
     4261 tggaggctac aagctggtgc cctgattaaa aaggccccag cacgaatgga taacttcgga
     4321 acattttgga ctgctttgaa tcgttatatg gtgccgggag ggggaatcga acccccacag
     4381 tgttaccacc ggcggatttt gagtccgctg cgtctaccaa ttccgccatc ccggcccggg
     4441 ttcgcttgag acgaaccgaa ggaggcggaa ttatccttga ttcatcactg accgacaagc
     4501 cgccctgcag acaattttac gcatgctgac actcgacgac ttcgactttt ccctgccgcc
     4561 cgaactgatt gcccagcatc cgacgccgga gcgctcggcg agccgcttgc tacatttggc
     4621 gggtgatgcg caacatgacc ggcagtttgc ggagctgcct caactcgtag aacccggcga
     4681 cctgctggtt ttcaacgata ccaaggtcat cagggctcgg ctcctcggtc aaaaggaaag
     4741 tggcggccgg atcgaggtaa tgatcgagcg aattgtcgat cgcgaccgtg ccgtcgcaca
     4801 gattcgcgcc agtaaatcgc caaagtcggg aagccgaatc gttctcgaaa acgcgattgt
     4861 ccttacggtt accggccgca ccggagagca atccgaattt ttcgccctcg aattggtggg
     4921 cgatggcgac cttttcgaat tggtagagca gtatggccat ctgccgctgc caccctacat
     4981 cacccacgca gccgggcctg aagacgaaac gcgctaccag accgtgtttg cccgccatgc
     5041 gggcgccgtg gcggcaccga cggccggcct gcacttcgat cacgcgacgc tggcacaact
     5101 cggcgaacgc ggtgcggaac tcgcctggct gaccctgcac gtgggtgccg gcacctttca
     5161 gccggtacga gtcaacaacc tcgccgagca ccacatgcat acagaacgtt ttgaaattcc
     5221 gcaagctacg gtcgacgcca tcgcccgcac ccgcgcacgt ggcggccgcg tcatcgccgt
     5281 cggcaccacc agcctgcgcg cgcttgaagc cgcagctcag aacgggaaac aaggcggcga
     5341 actgcaggcc ggagcggccg aaaccgatat tttcatcacg ccgggttacc gcttccgggt
     5401 tgtagaccgg ctgatcacca acttccattt gccaaaatcg acgctgctca tgctcgtctc
     5461 ggcgttcgcg ggtactgata tcatccgcgc cgcctaccgt catgccatag ccgaacggta
     5521 tcgtttcttc agttatggtg acgccatgct tctcgaaaaa ttggacgaaa attcgcacga
     5581 aaatgcaaac aatgcaattt gaacttctcg ccgcggatgg tgacgcccgg cgcggtcgcc
     5641 tgatgctgac gcacggcgtc gtcgacacgc ccgtgttcat gccagtgggc acttacggca
     5701 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacggcttt gccatcggcg
     5821 gactctcggt tggcgaaccg aaagacgaaa tggcccgcat cctcgcacac actgcaccac
     5881 gcctgccgca gaacaagccg cgatatctga tgggcgtcgg cacgccggaa gacctggtgg
     5941 ctgccgtact caccggaatc gacatgttcg attgcgtgat gccgacgcga aacgcccgca
     6001 acggccacct gttcacccgc tacggcgaca tcaagatcaa gaatgcccag tacaagaacg
     6061 acccgcgtcc actcgacgag ggctgcgaat gctacacctg ccgcaatttc tcgcgcgcct
     6121 acctgcacca cctgcaccgc gttggcgaaa tcctcggcgc acagctcaac accctgcaca
     6181 acctgcacta ctaccagacc ttgatgggcg agctgcgcaa ggccatcggc accggggaac
     6241 ttgcccgaac tgtcgcccgt ttccatgccg ggcgcagcga tcggggcgga gtgatacaat
     6301 agcgcgctaa ttttctgacg ctttcacgtt ttcgtcttcc aaggagattc atgtgctgat
     6361 cagcaacgct tacgctcaaa cggctgccgc cgccggcccg ttggacaatg tcatgcagtt
     6421 cctgccgatc attctgatgt tcggcgtact ctacttcctg atgatccggc cgcaaatgaa
     6481 gaaagccaag gagcacaaag cactgcttga tgcgctgggc aagggcgacg aagtcgttac
     6541 gcagggcggc atcgctggcc gcatcacaaa agttggcgag gacttcgtca cgatggcaat
     6601 ttccgacaac gttgaaatcc agatgcagaa gccggcaatt accctggttc tgcccaaagg
     6661 cacgctgaaa agcctgtgag gcgtgaggtg tgaatagtgc ggagaaaacc ctccgctccg
     6721 caatccaact cccaactcct gactcctctc cgcccctatg aaccgctatc cgctgtggaa
     6781 atacatcatc gtcggcttcg cgctggtgtt cggctttatc tataccttgc cgaacttctt
     6841 tggcgagtcg ccggcggtgc aggtgtccag cgccaaagcc acgatcaagg tcgatatcaa
     6901 gacactggaa cgcgtcgaga gcacactcaa ggaagcgagt gtcgaaaact ttggcatctt
     6961 cctcgacacc aacggcgtca aggttcgcct gaaagacacg gatacccagc tcaaggccag
     7021 ggatctgctc gaaaagcagt tcaaccccga ccccactgac ccgcagtacg tcgttgcact
     7081 gaatctgctt tcgagttcgc cgcagtggct gacccgtatg ggtgcattgc cgatgtacct
     7141 gggcctcgac ctgcgcggtg gcgtgcactt cctcctccag gtcgacatga aaggggcgct
     7201 gaccaagcgt ctcgattcgc ttagcgccga cctgcgcagc cagttgcgcg acaaaaccat
     7261 tcgtcacggc ggcatcaacc gagaaggcga acgtctggtc atccgcttcc gcgacgagga
     7321 aacccgcaac aaggggcgcc tggcagtgga agacagtcag cccgatctgt tgcttgccga
     7381 tcagggtgag ggcacggacc tcaagctggt cgccacgctt tcgccggctg cgatgaagcg
     7441 catccaggaa ttcgccatca agcagaacat gggcaccctg cacaaccgga tcaatgagct
     7501 cggcgtcgcc gagccggtga tcgcgcaaca gggcgccgac cgcatcgtgg tccagttgcc
     7561 tggggtacag gacacggcca aggccaagga tattctgggg cgcactgcga cgctggaaat
     7621 ccgcatggtc gacgacactc ccggtgcgct ggaagccgcg atggccgggc aggtgccttt
     7681 cggcaccgag ctttacgtcg agcgcggcgg caggccgttg ctggtcaaga aacaagtggt
     7741 attgaccggc gaccgtctga cggacgccca gccaggcttc gacagccaaa cccaggaagc
     7801 ggcggtgcac ctgaacctgg actcggccgg atcgcgcatt ttcagggata tcacacgcga
     7861 aaacgtgggc aagcgcatgg ccatcctgct cattgaaaaa ggcaagggtg aagtcgttac
     7921 ggcacccgtc atccgtacgg aaatcggcgg cggacgcgta cagatttccg gcagcatgag
     7981 caccatggaa gccaacga
//
LOCUS       DAORPM010000100         7931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2261_length_7931_cov_9.775013, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000100 DAORPM010000000
VERSION     DAORPM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_7931_cov_9.775013"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLD53_06355"
     CDS             complement(<1..328)
                     /locus_tag="PLD53_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HRC60568.1"
                     /translation="MPEASDRAALGIFGGTFDPVHFGHLRLAEEAADSLGLERVRWIP
                     AGRPALRDSPEVSAEQRLEMVRLAISGNPRFELDSAEVNDARPSYTVPTLERLRTTEE
                     FGPQQPL"
     gene            complement(321..1097)
                     /locus_tag="PLD53_06360"
     CDS             complement(321..1097)
                     /locus_tag="PLD53_06360"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HRC60569.1"
                     /translation="MKALTLPAGRGWHWLSGGFRLFRKGRLVLSLAVLGYWLCMALIN
                     SFPIVGQIAASLLVPAFSVGLMNVCRLIEQGGTLQARELFSGLRQNQRTLLVLGAIYI
                     VAGVCVLGFVSLIDGGVLFQAIVLGDRLDERTLSSGEFVLAVQVALLLFSPIMMTYLF
                     APVLAVWHGFPAGKSLFFSFFACARNWRAFVAYAVAVILYGALLPGLLVGLLVSVIPG
                     GSELFSVLLPMLIMLIFLPTLYASFYVSYRDIFVSIDENA"
     gene            complement(1181..1918)
                     /locus_tag="PLD53_06365"
     CDS             complement(1181..1918)
                     /locus_tag="PLD53_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HRC60570.1"
                     /translation="METFPIQAPAFNGNSRAVPASNAFEWLKQGWALFIVNPGLWIAL
                     TIVLLVILLGLNIVPMIGTLAANLLMPVLSAGMLLACQKVSNGEELEIADLFAGFKQN
                     TGDMIMLGVLYMVAMLIIVVVVIVLGGGSMAGGVLMGRPAGVGLAFGGMLLAMLLWLV
                     LSVPLFMAIWFAPALVFFNRMAPVDALKASFNACLKNFLSLLVYGLIVMVLMFFAALP
                     AFLGFLVLIPVLFGSVYVSYRDIFVAN"
     gene            complement(2059..2400)
                     /locus_tag="PLD53_06370"
     CDS             complement(2059..2400)
                     /locus_tag="PLD53_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HRC60571.1"
                     /translation="MRRTHAFIALLALVAVPALAQAPAAEPPPVPDVRPPAMVPLDDS
                     IEPQVTIRKREGSTVEEYRINGRLYKIIVTPEHGVPYTLVDQQGDGSFAIQDSPGTPT
                     LSVPMWVIGTF"
     gene            complement(2442..3167)
                     /locus_tag="PLD53_06375"
     CDS             complement(2442..3167)
                     /locus_tag="PLD53_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRC60572.1"
                     /translation="MSAKEKILPVADTNASKISAEHEAWRVFDIMSEFVEATQRLSAI
                     RPAVSIFGSSRIQSDSPFYKLTEDIARQLSDAGFSVISGGGPGVMEAANKGAFHGKSL
                     SVGLNIQLPHEQTSNEFQDISQSFRHFFARKYMFVRFANAYVVMPGGFGTLDELHEAL
                     TLVQTGKSQRIPIILVHEPFWRGLVDWFRSTLVDEKLISPEDLDLIQVIDKPAEVVEA
                     IFRHYENRCFAPLPSERELMLNL"
     gene            3185..5947
                     /gene="polA"
                     /locus_tag="PLD53_06380"
     CDS             3185..5947
                     /gene="polA"
                     /locus_tag="PLD53_06380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRC60573.1"
                     /translation="MPILLLVDGSSYLYRAFHAMPDLRNSQGEPTGAIYGVLNMLRRL
                     DSDYKAQDIVYKACVFDAKGKTFRDDWYADYKATRPAMPADLVRQIEPIHVGVAASGW
                     PVLMIDGVEADDVIGTLARQAEALGIQVVISTGDKDLAQLVSPKITLINTMSNEMLDE
                     AGVLAKFGVPPERVVDYLTLVGDTVDNVPGVEKVGPKTAVKWLSQYGSLDAVIAAAAE
                     IGGAVGKNLRNAVDFLPLGRKLVTVRCDLELPVSIDDLKPREADRSQLAELFLRYEMK
                     SWLKEVRESADKPLVDSAEASRQEPPAEAPVANAASGDHRKNYACILDWAGFESWLKK
                     IEHSPLTSLDTETTSLDPFAARLVGISLAVSPGEAVYLPLSHDYAGAPAQLELETVLK
                     RLKPWLESEQHAKIGQNLKYDQHVFANHGIALAGIAHDTLLQSYVLESGKDGVRGHDL
                     GRLALRHLGLATIAFEDICGKGASQIGFNQVAVDQATDYAAEDADLCLLLHQKCFPQI
                     AANKGLERIYGEIEMPVREILFRVERTGVLIDSGLLAQQSQELGERLIELEALAHEAA
                     GQPFNVNSPKQLAEILFDKLGLPVKKKTPSGTPSTDEEVLSELALDYPLPKLLLESRQ
                     LSKLKSTYTDKLPKMVNRQTGRVHTSYAQAVAVTGRLASSDPNLQNIPVRTAEGRRIR
                     AAFIAPPGSHIVSADYSQIELRIMAHLSEDARLLEAFAQGEDVHRATAAEIFGVTPIE
                     VGADQRRVAKVINFGLIYGMSAFGLARQLDIERSAAQAYIERYFTRYPGVAKYMESTR
                     SAAKANGFVETVFGRRLWLPEIHASQAARRQGAERAAINAPMQGTAADLIKLAMIAVQ
                     RWIDSDSLKTRLVMQVHDELVLEVPDEELARVRVELPPHMTQVARLNVPLVVEVGVGD
                     NWDEAH"
     gene            5970..7304
                     /locus_tag="PLD53_06385"
     CDS             5970..7304
                     /locus_tag="PLD53_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60574.1"
                     /translation="MPLMNTLSAIADGMFFAQGSTLLRNLFLLVFVSAASICTAARAA
                     EQLPVSPVYKGEIRRGADGKLRVEASTDPLKPAVDDSAKPATMRVGSQERITTISEAA
                     KLARDGEVIEIRPGSYRGQPAVWTQNNLVIRGSGERPVMLADGKSAEDKAIWVIKGGN
                     VRIENIEFRGARVPSGNGAGIRFERGHLVVTRCTFVDNEMGILTSNLADMTLEVSDSE
                     FSAAPHDMADLHHLLYVGAIRKFTLSGSRFEQGYLGHLVKSRARENIVRNNMLADGMS
                     GRSSYELEFPNGGIAYVLGNVIGQSAGTDNPVLVSYGAEGPRWPVNKLFLVHNTLVND
                     FHTGTFVQLWDEKFPGGIESWIINNLTVGYGDLNQPAHGRFEGNQSALRRDLIDYGGL
                     PVRLNSSSPLRGSVRIPGQANGMDLIPAAEFSFPVGTKPIRIGNSLAPGAFQ"
     gene            complement(7326..>7931)
                     /locus_tag="PLD53_06390"
     CDS             complement(7326..>7931)
                     /locus_tag="PLD53_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HRC60575.1"
                     /translation="LGADGHDFEPIVNELDIDSLPGIRFIFPHAPMRPVTINGGFVMR
                     AWYDITSPDFSQRREDERGVRESAGQIEALIARENALGIADDHIVLAGFSQGGAIALH
                     TGLRHTQRLAGVLALSTYLPLDDTLTAEANTANRDVPIFMAHGHGDTVIPYEMGLRSS
                     ELLKTQGYPLTWHPYFAEHTVCMEELRDIETWLHEILGGTT"
BASE COUNT         1866 a         2275 c         2292 g         1498 t
ORIGIN      
        1 ccagtggctg ctgcggcccg aactcctcgg tcgttcgcag gcgttcaagg gtcggtacgg
       61 tgtagctagg tcgggcatcg ttgacctccg ccgaatccag ctcgaaacgc gggttgccgg
      121 aaatggccag gcgaaccatc tcgagccgtt gctcggcact cacttccgga gagtctcgta
      181 aggccggccg gccagccgga atccagcgga cgcgttccag tcccaagcta tctgcagcct
      241 cctcggcgag ccgcaagtgt ccgaagtgaa ccggatcgaa ggttccgccg aagatgccga
      301 gcgcagcccg gtcggaagct tcaggcattc tcgtcaatgg aaacaaaaat gtctcgatag
      361 ctcacgtaga aactggcgta tagcgtaggc agaaaaatga gcatgatgag catcggcaac
      421 agcacagaga acagttcgct tcctccgggg atcactgaaa caagcaaacc gacgagcaaa
      481 cccggcagaa gcgcgccata tagtatgacc gccacggcat aggcaacaaa tgcgcgccag
      541 tttcgtgcac aggcaaaaaa gctgaagaac agcgacttgc ctgcggggaa accatgccag
      601 acagccagca cgggcgcgaa caagtacgtc atcatgatcg gcgagaagag caacagggcg
      661 acttgcaccg caagaacaaa ctcgccactg gaaagcgtgc gttcgtcgag tcggtcacca
      721 aggacaatgg cctgaaagag cacacctcca tcgatcaatg atacgaatcc gagaacgcag
      781 acaccggcaa caatgtagat ggccccaaga acaagcaggg tgcgctgatt ttggcgaagt
      841 ccggagaaca gttcccgcgc ttgaagcgtg ccaccctgct cgatcagacg acagacattc
      901 atcaagccga ccgagaaagc cgggacaagc agagatgctg cgatctgtcc gacaatcggg
      961 aacgaattga tcaaggccat acacagccag tagccgagca cggcgagtga aagcaccagt
     1021 ctacctttgc ggaatagtct gaatccgccg gacagccaat gccagccgcg ccctgcagga
     1081 agagtcagcg ctttcatgga acccggtcag tgaagcgagt caggagagga acgaaggcgc
     1141 acaaggacga ccggattcaa ggaaatggtg cacgcactgt tcagttggca acaaaaatat
     1201 cccggtagga cacatacacc gatccgaaaa gaaccggaat cagcacaagg aagccgagga
     1261 aggccggcag tgcggcaaag aacatcagca ccatgacgat caaaccgtag accagcaacg
     1321 acaggaaatt cttcaggcag gcattgaagc tggccttgag cgcgtcaacc ggcgccattc
     1381 tattgaagaa caccagcgcc ggcgcgaacc agatggccat gaataacggc accgagagta
     1441 ccaaccacag caacatggcg agcagcatgc caccgaaagc caagccgact ccggcagggc
     1501 ggcccatcag cacaccaccc gccatgctgc caccacccag aacgattacg acgacgacga
     1561 tgatcaacat ggccaccatg tagagcacgc caagcatgat catgtcgccg gtattctgct
     1621 tgaatccggc aaacaggtcg gcgatctcga gctcttcccc gtttgacact ttctggcacg
     1681 ccagaagcat gcctgcggaa agtaccggca tcagaaggtt ggcggccagg gtgccgatca
     1741 tgggaacgat attgagtcca agaagaatga ccaacaagac gatggtcaat gcgatccaca
     1801 aaccggggtt gacgataaac agcgcccagc cctgcttgag ccattcaaag gcattgctgg
     1861 caggtacggc gcgactattg ccgttaaagg cgggagcttg aatcggaaag gtttccatga
     1921 ctttattcgc tcaaatcagt tgaatgatga agtacgaaag tgcagcgcct gagcatgttt
     1981 ttccggacat ttcaagcgca cattgtcgca atccagccgg ctctgagatg agtagcggca
     2041 agtaaccggc acagcaagtc agaaggtgcc aatgacccac attggcacgc tcagcgttgg
     2101 ggttcccggg gaatcctgga tcgcaaaaga accgtcgccc tgctgatcca ccagcgtata
     2161 gggaacgccg tgttccggcg taactatgat cttgtaaagc ctgccgttga tgcggtattc
     2221 ctcgaccgtg ctgccttcgc gtttcctgat ggtcacctgc ggctcgattg agtcatcgag
     2281 cggcaccatg gccggcggcc ggacatccgg cacgggtggc ggctcagcgg caggggcctg
     2341 cgccagagcc gggacggcga caagggccag cagggcaatg aaggcatgag tacggcgcat
     2401 gaagttactt tccagttggc tgtagatcag ccttgattgt attacagatt gagcatcaac
     2461 tcgcgttccg acgggagtgg agcgaagcac cggttttcgt aatgtctgaa aattgcttcg
     2521 accacttctg caggcttgtc gatcacctgg atcaaatcga gatcctcggg cgatatgagc
     2581 ttttcgtcga ccagcgtgga gcgaaaccag tcgaccaacc cgcgccagaa gggctcatgt
     2641 accaggataa tcggaatccg ctggctcttt ccggtctgta cgagagtcaa ggcttcatgc
     2701 agttcgtcaa gcgttccaaa accaccaggc attacgacat aggcattggc aaagcgcacg
     2761 aacatgtact tgcgcgcgaa gaagtggcga aagctctgcg atatgtcctg gaactcgttg
     2821 ctcgtctgct cgtgcggcaa ctgaatgttg aggccgacgc tgagcgactt gccgtggaac
     2881 gcccccttgt tggctgcctc catgacgccg gggccgcctc cggagatcac cgagaagccg
     2941 gcatcggaca gttggcgggc gatgtcttcg gtcagcttgt aaaatggcga atccgactgg
     3001 atccgcgaac tgccgaagat cgataccgca ggccgaatgg ccgacagacg ctgcgtggct
     3061 tcgacgaact ctgacataat atcgaacacc cgccaggcct cgtgctcagc actgatcttg
     3121 gatgcgttcg tatcggcgac cgggagtatt ttttctttag cgctcatttc atcttttcga
     3181 atatatgcca attttgctgc tggtcgacgg atcgtcgtat ctctaccggg cgtttcacgc
     3241 catgccggac ctgcgcaatt cacaaggcga accaacgggc gcgatctacg gcgtactgaa
     3301 catgctacgc cggctggata gcgactacaa ggcgcaggac attgtttaca aggcatgcgt
     3361 tttcgacgcc aagggcaaga cattccgcga cgactggtat gccgactata aggccactcg
     3421 accggcgatg cctgcggatc tggtccgcca gatcgaaccg atacacgtcg gtgttgccgc
     3481 ttcgggctgg ccggtgctga tgatcgacgg cgtcgaagcc gacgacgtga tcggcaccct
     3541 ggcgaggcaa gccgaggcac tgggaatcca ggtggttatc tcgaccggcg acaaggacct
     3601 cgcacaactt gtcagcccca agattacgct gatcaatacc atgagcaacg aaatgctcga
     3661 cgaagccggt gtgctagcca agtttggcgt gcctccggag cgcgtcgtcg actacctgac
     3721 gctggtcggc gacaccgtgg ataacgtacc cggtgttgaa aaggtcggcc cgaaaacggc
     3781 ggtcaagtgg ttgtcgcagt acgggtcgct tgacgccgta atcgctgcag ctgctgaaat
     3841 tggcggggca gtcggaaaga acctgcgcaa tgctgtcgat ttcctgccgc ttgggcgaaa
     3901 gctcgtcacc gttcgttgcg acctcgaact gcccgtgtcg attgacgacc tcaaaccgcg
     3961 cgaggccgat cggtcgcaac tggcggagct tttcttgcgc tacgaaatga aatcctggct
     4021 gaaggaagtg cgggagtctg cggacaagcc gttggtggat tcagccgaag cctcgcgtca
     4081 agagcccccg gcggaagcac ccgtagcgaa cgcagcttcc ggcgatcacc gcaagaacta
     4141 cgcgtgcatc ctcgactggg ccggcttcga atcatggctc aagaagattg aacactcgcc
     4201 gctaacctcg ctcgacactg aaacgaccag cctggatcct tttgcggctc gtctcgtcgg
     4261 catttcgctg gctgtgtcgc ccggcgaagc cgtttatctt ccactaagtc acgactatgc
     4321 cggcgcgccc gcccagcttg agctggagac cgtgctcaaa cgccttaagc cgtggctcga
     4381 atcggaacag cacgccaaaa tcggtcagaa tctgaaatac gatcagcatg tgtttgccaa
     4441 ccatggtatc gcgctggcag gtatcgccca cgacacgctg ctccagtcgt acgtgctcga
     4501 atcgggcaag gacggcgtgc gcggccacga tctcggacga cttgcactac gccatcttgg
     4561 cctggcaacc attgccttcg aagacatttg cggcaagggg gcgagccaga tcggattcaa
     4621 tcaggtggct gtcgaccaag ccaccgacta cgcagcagaa gacgcggacc tttgtctgct
     4681 tttgcaccag aaatgcttcc cgcagatcgc tgcaaacaag ggattggaac gcatctacgg
     4741 cgagatcgaa atgccagtgc gcgagattct tttccgcgtc gagcgcaccg gcgttctcat
     4801 tgactccgga ctgctcgcgc agcagagtca ggaactcggc gaacgcctga ttgaactcga
     4861 agcactggcg cacgaggctg ccggccaacc gttcaacgtc aattcaccga aacagcttgc
     4921 cgagatcctg tttgacaagc ttggcttgcc ggtgaaaaag aaaacgccgt caggcacgcc
     4981 gtcaaccgac gaggaagtgc tctccgaact ggcgctcgac tacccgctgc ccaaattgct
     5041 tcttgaatcg agacagcttt ccaaactgaa aagcacctac accgacaagc tgccgaagat
     5101 ggtcaaccgc caaaccggcc gcgttcacac cagctacgca caagccgtgg cggtgaccgg
     5161 acgactggct tcaagcgacc cgaatctgca gaacataccg gtacgcaccg ccgagggaag
     5221 acgcattcgc gccgccttta tcgcgccacc gggcagccat atcgtgtcag ccgactactc
     5281 gcagatcgag ttgcgcatta tggcgcacct ctcggaagac gcgcgcctgc tggaggcttt
     5341 tgcacaggga gaggatgtac atcgtgccac ggctgccgag atattcggcg tcacgccaat
     5401 cgaggtgggt gccgatcaac gtcgtgtcgc caaggttatc aacttcggcc tgatctacgg
     5461 catgagcgcc tttggcctcg cccgccagct tgacatcgaa cgcagtgcgg cacaggccta
     5521 tatcgagcgt tactttacgc gctatccagg cgttgccaaa tacatggaaa gcacccgcag
     5581 cgcggcaaaa gcaaatggct ttgtcgaaac tgtctttggt cgtcgcctgt ggctgccgga
     5641 gatccacgcc agtcaggccg cgcgccgcca aggcgccgaa cgcgcggcaa tcaatgcgcc
     5701 gatgcaaggc accgccgccg atctgatcaa actcgcgatg attgccgtgc aacgctggat
     5761 cgactccgat agtctgaaga ctcgcctagt gatgcaggta catgacgaac ttgtgctcga
     5821 agtccctgac gaagagctgg ccagagttcg cgtcgaacta ccaccgcaca tgacacaggt
     5881 cgccaggctt aacgtgccgc tggtcgtcga agttggcgtg ggcgacaact gggatgaggc
     5941 gcattgaaac agcccaaggg gagaaacgaa tgccgctaat gaatacgcta agcgctattg
     6001 ccgatggcat gttctttgcc caaggcagca cgctgctgcg aaacttgttc ttgttggtgt
     6061 ttgtgtctgc cgcctcaatc tgcacagcgg cgcgtgctgc agaacagctt cccgtatcgc
     6121 ccgtttacaa gggagagatt cggcggggtg cggacggcaa gttacgggtc gaggcgagta
     6181 ccgatccgct caaaccagct gtcgacgact ctgcaaaacc agcaaccatg cgcgtcggca
     6241 gccaggaaag gatcaccacg atctccgagg cggcgaagct ggcccgtgac ggagaggtga
     6301 tcgagatccg cccagggtcg tatcgtggac aaccggccgt gtggacccag aacaatctgg
     6361 tcatccgtgg ttcgggtgag cgtccagtga tgctggccga cggcaagagt gccgaagaca
     6421 aggccatctg ggtgatcaag ggcggcaacg tgcgaataga gaacatcgag ttccgggggg
     6481 cacgcgtgcc gtcgggcaac ggggcaggca ttcgtttcga gcgagggcat ctcgttgtaa
     6541 cccggtgcac ctttgtcgac aacgagatgg ggatactcac gtcgaatcta gcggatatga
     6601 cccttgaggt tagcgatagc gaattttccg ccgcaccaca cgatatggct gatctgcatc
     6661 acctgctcta tgtcggcgcg atcagaaaat ttacgctttc tggaagccgc ttcgaacaag
     6721 ggtacttggg acatctggtc aaatctcgcg ccagggagaa tattgtgcgc aacaacatgc
     6781 tagccgatgg catgtcgggt cgctcttcgt acgagctgga gtttcccaat ggaggaattg
     6841 cctatgtgct cggcaacgtg atcggccaga gtgccggaac ggataacccc gtcctggtct
     6901 catatggcgc cgaaggacca cgctggccgg tcaacaaact cttcctggtt cacaacaccc
     6961 tggtcaatga ttttcacacc ggaactttcg tccaactttg ggatgagaag ttccctgggg
     7021 gtatcgagag ctggatcatc aataatctga cggttgggta tggcgatctg aatcagccgg
     7081 cccatggccg gttcgaaggc aatcaatcgg cactacgtcg cgatctgatc gattatgggg
     7141 ggctgccggt acgactgaac agctcttctc cgttgcgcgg atcggtcagg attccaggac
     7201 aggccaatgg catggatctg attccggctg ctgaattcag tttcccggta ggcaccaaac
     7261 ccattcgtat tggcaattca ctggcgcctg gagcatttca gtgagcacca atacaggagc
     7321 aacgatcagg tcgtgccgcc aagtatctcg tgcaaccaag tctcgatgtc gcgcaactct
     7381 tccatacaga ccgtgtgctc ggcaaagtag ggatgccagg tcagcgggta gccctgagtc
     7441 ttgagcagtt cactcgagcg caaacccatc tcatacggaa tcaccgtatc gccatggccg
     7501 tgagccatga atatcggcac atcgcgattt gctgtgtttg cctcggccgt caaggtgtca
     7561 tccaaaggca agtacgtcga tagagcaagt actcccgcca gcctctgggt gtgccgcaag
     7621 ccggtgtgca gtgctatcgc gccaccctgg gaaaaaccag cgagcacaat gtggtcatcg
     7681 gcgataccca gtgcgttttc gcgggcaatc aatgcttcaa tctgtccggc agattcacgc
     7741 accccccgct cgtcttcgcg tcgttgtgag aaatcgggcg atgtaatatc gtaccaggca
     7801 cgcattacaa agcctccatt gatggttact ggccgcatcg gcgcatgggg aaagatgaag
     7861 cggataccgg gaagcgaatc aatgtccagt tcattgacga tgggctcaaa gtcgtgcccg
     7921 tcggctccca a
//
LOCUS       DAORPM010000101         7713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2357_length_7713_cov_17.761426, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000101 DAORPM010000000
VERSION     DAORPM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_7713_cov_17.761426"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLD53_06395"
     CDS             complement(<1..652)
                     /locus_tag="PLD53_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC60576.1"
                     /translation="MIDGITVRTIPTGGYVCMKGEPVEYWMGILDGLVKMASIWSTGK
                     ATSFLGLSTGGWFGEGSLLKDEPRKYDVIALRETRVAYMNRATFQWLHDNSIHFNRFL
                     LIQLNERLGQFIGMMEHERLLDTDSRLARCLASLYNPVLYPESNLEIRISQEEIGLLA
                     GISRQRVNQALRKLEDAGLLQIDYGSITILDLPGLRRFGESSAQKRNSLLPGRRGHP"
     gene            958..2916
                     /locus_tag="PLD53_06400"
     CDS             958..2916
                     /locus_tag="PLD53_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC60577.1"
                     /translation="MPEMASSRAMDTFPRLLFYHSQERGTKTAMREKDLGIWQTWSWS
                     EVAEQVGALACGLAEMGFKRGDNLAIIGDNRPRLYMTMAAVQCLGGVPVPLYQDGVAF
                     EMLFVMQDAAIRYALVEDQEQVDKLLEIKDQLPQLKLIGYDDPRGMRHYDQPFIHDIE
                     ALMAMGRVFQQNNPGFLDAEVAQGGSDDISVMLYTSGTTGKPKGVCLTHNAFISAARG
                     GIGFDGLSADEDILSYLPMAWVGDHLFSYAQAMYAGFTINCPESGDTVMNDLREIGPT
                     YYFAPPRIFENLLTQVMIRMEDASVIKQKLFHYFMKVARRCGADVLDGKPVPAGDRVL
                     YALGNILIFAPLKNVLGLSRVRVAYTAGAAIGPDLFRFYRSIGINLKQLYGQTETCAY
                     VCLQPDGEIKFDSVGKPAPGVEVKIADNSEILVKGPMLLKEYYKRPEATAESINDEGY
                     FMTGDAGLIDEEGHLRIIDRAKDVGKLANGAMYAPNYIENKLKFFQHIKEAVVFGDQR
                     KMVCAFINIDIGAVGNWAERRGIAYSGYTDLAAKPEVYQLIQDCIEKVNGELVGEGAL
                     ADSQIHRFLILHKELDPDDDELTRTRKVRRDFVAQKYAVLIDALYSGKSSQFIETAVK
                     FEDGRQGKVAADLLIRDIKTFGIAGRAA"
     gene            2916..3698
                     /locus_tag="PLD53_06405"
     CDS             2916..3698
                     /locus_tag="PLD53_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC60578.1"
                     /translation="MSRQIGEVILDLKGVSLAFGGVKALSDISFDVREHEIRAIIGPN
                     GAGKSSMLNVINGVYRPQQGEIILRGEHFARMNPYKAAKAGISRTFQNIALFKGMNVI
                     DNMMTGRNLKIRSNLIQQAFWWGPARREELAHRAKVEEVIDFLEIQHIRKTPVGRLPY
                     GLQKRVDLGRALAMEPSVLLLDEPMAGMNVEEKQDMCRFILDINDQFGTTIVLIEHDM
                     AVVMDISDRVVVLDYGKKIGDGDPDEVRASPEVISAYLGVSH"
     gene            3710..4639
                     /locus_tag="PLD53_06410"
     CDS             3710..4639
                     /locus_tag="PLD53_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC60579.1"
                     /translation="MGFFLETLIGGLMAGMLYSLIALGFVLIYKASGVFNFAQGAMVL
                     FAALAMARFAEWLPGWLGLESLWLANTIAFVVAAALMFVVAWLIERLVLRYLVNQEGA
                     TLLMATLGIAYVLEGVGSSLFGNDVYRIDVGMPKDPVLILDGIFEGGLLINKEDFITA
                     LIAALLVGLLTIFFQKTTTGRALRAVADDHQAAQSIGIPLNHIWVIIWSVAGLVALVA
                     GVIWGSKLGVQFSLATVALRGLPVVILGGLTSIPGAIIGGLIIGVGEKLSEVYLGPLI
                     GGGIEIWFAYQLALVFLLFRPQGLFGEKIIDRV"
     gene            4655..5731
                     /locus_tag="PLD53_06415"
     CDS             4655..5731
                     /locus_tag="PLD53_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRC60580.1"
                     /translation="MLYRENGQFKTTYAADQQLFPIDQDRWSILGVVLFAFIAVPLLV
                     DEYMFRAILIPFLILSLAAVGVNILVGYCGQITLGAGAFMAVGAYAAYNFHIRIDGLP
                     LIVSILLGGLTSALVGIFFGIPSLRVRGLYLAVATLAAQFFTDWVFLRIPWFTDNSSS
                     GSVSVSNLQVMGWQINNPVSKYLFCLFFLTVFALLAKNMTRCAIGRQWMAIRDMDVAA
                     EVIGIRPLVAKLSAFAVSSFFVGTAGALWGFVHLASWEPAAFSVDRSFQLLFMVIIGG
                     LGTIMGSFFGAAFIVILPIALNQLLPAFGGLIGVEISTAGVSHAELVIFGGLIVFFLI
                     KEPHGIARLWSTGKEKLRLWPFPH"
     gene            5827..7161
                     /locus_tag="PLD53_06420"
     CDS             5827..7161
                     /locus_tag="PLD53_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC60581.1"
                     /translation="MNLRKLALAASLSAVVLSSLATAPANAAAKEQFFPALVYRTGAY
                     AANGAPWANGYLDYLKLVNAKGGINGVKVVFEECETGYATDRGVECYERLKGKHGGAT
                     VIQPLSTGITFALTEKAPKDKIPLITAGYGRSESSNGAVFTWNFPLLGTYWVGADVAI
                     QYIAQKEGGIAKLKGKKIALVYHDSPYGKEPIALLEELSKKHGFDVQLLPVAHPGVEQ
                     KATWLQIRQGRPDYVLLWGWGVMNSTALREAQATGYPRNKMLGVWWSGADPDVKDVGE
                     GAKGYSALTLQDGADSNSKIVKDILAAVHAKGDGTGPKDEVGSILYMRGLISAMLGVE
                     GVRKAQERYGKGKVMTGEQVRWGLENLNLDQATLDAMGFAGVMRPVQTSCLDHMGSSW
                     VRIRTWNGSKWETSSDWFQADEKTLRPMVMLASGKYADDKKLTPRTPEECKM"
     gene            7265..>7713
                     /locus_tag="PLD53_06425"
     CDS             7265..>7713
                     /locus_tag="PLD53_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC60582.1"
                     /translation="MSDANIILRVNSIEVIYNHVILVLKGVSFSVPEGGIVALLGGNG
                     AGKTTTLRAVSNLLRGERGEVTKGTIELRDERIEALTPAQLVKRGVIQVMEGRHCFAH
                     LSIEDNLLTGTYTRTDGKAAIAETLDKVYTYFPRLKTRRSSQAAYTSG"
BASE COUNT         1530 a         2180 c         2350 g         1653 t
ORIGIN      
        1 aagggtgccc tcggcgtccg ggcagcaacg aatttcgctt ctgcgcagac gactcgccga
       61 agcggcgcag gccgggaagg tcgagaatgg tgatgctgcc atagtcgatt tgcagcagcc
      121 cggcatcttc cagtttgcgt agcgcctgat tgacgcgctg ccgcgagatg ccggcaagca
      181 gaccgatttc ctcttgcgat atccttattt ccagattgct ttcggggtag agcaccgggt
      241 tgtacagcga ggcgaggcat cgggccaggc gggaatcggt gtcgagcagg cgctcgtgtt
      301 ccatcatccc gataaactgg ccaagtcgtt cgttgagctg gatcagcagg aagcggttga
      361 aatgaatgct gttgtcgtgc agccactgga aagtggcgcg gttcatatag gccacccggg
      421 tttcgcgcag ggcgattacg tcgtacttcc ggggctcgtc cttgagcagc gaaccctcac
      481 cgaaccatcc accagtcgac agaccgagga aagacgtcgc ttttccggtg ctccagatgc
      541 tcgccatctt gaccaggccg tcgaggatac ccatccagta ttcgaccggc tctcctttca
      601 tgcatacata tccgccggtg ggaatggtgc gaacggtgat gccgtcaatc acgctgcgca
      661 attgattttc cgacagttca cgtgcccagc tcgaggccag cagcatttcc tttaacgacc
      721 gcataatttt tgtgccaatt gtcaggtagg cgacaattat agtgcccagc tcaacttaga
      781 ctgcgcgttg aaccggtttc tgtgccatcc tgctagcgat gagcctggga ggcattacga
      841 tcagtccggg cagcgaaatt tccgcttgca acaatactga atatcttgac tggattttag
      901 gacgacaact cactattctt cacgctgatg acggttaatt gaaccgaggg gaaatatatg
      961 ccagagatgg cgtcttcccg ggcgatggat acctttcctc gcctgttgtt ttaccattct
     1021 caggagcgcg gcacgaaaac cgcgatgcgc gagaaggatc tcggcatctg gcagacgtgg
     1081 tcgtggagcg aggttgccga gcaggtgggc gccttggcgt gcggcttggc cgagatgggc
     1141 ttcaagcgcg gtgacaacct ggcgatcatc ggcgacaacc ggccccgcct gtacatgacc
     1201 atggctgccg tgcagtgcct cggtggcgtt ccggttccgc tgtatcagga tggcgtcgcc
     1261 ttcgaaatgc tgttcgtgat gcaggacgcc gccatccgct acgcgctggt cgaggatcag
     1321 gagcaggtcg acaaactgct tgagatcaag gaccaactgc cgcagctcaa gctcatcggc
     1381 tacgacgatc cgcgggggat gcgccactac gatcagccgt tcatccatga catcgaagcg
     1441 ttgatggcga tggggcgggt ctttcagcag aataaccccg ggttcctcga cgcggaagtg
     1501 gcgcagggtg ggagcgacga catcagcgtc atgctctaca cgtccggtac gacgggcaag
     1561 cccaagggcg tttgtctgac ccacaacgcg tttatcagcg cggcacgcgg tggcatcggg
     1621 tttgacggcc tgagcgcaga cgaagacatc ctttcgtatc tgccgatggc ctgggtgggc
     1681 gaccacctgt tttcgtatgc gcaggccatg tatgccggct tcaccatcaa ctgcccggaa
     1741 tccggcgata cggtgatgaa cgatctgcgc gaaatcgggc caacctacta ctttgcaccg
     1801 ccacgcatat tcgagaatct gctgacgcag gtgatgatcc gcatggaaga cgccagcgtc
     1861 atcaagcaga agctgtttca ctatttcatg aaggtcgcgc ggcgttgcgg tgccgatgtc
     1921 ctcgatggca agccggttcc ggccggtgat cgcgtgcttt acgcactggg caacattctg
     1981 attttcgccc ccctgaagaa cgtgctgggt ctgagccggg tgcgggtggc ctataccgcc
     2041 ggcgcggcca tcgggcctga cctctttcgc ttttatcgtt cgatcggcat caatctgaag
     2101 cagctctacg ggcagaccga aacctgtgcc tacgtttgcc tgcagcccga cggcgagatc
     2161 aagttcgaca gcgtgggcaa gccggcgccg ggcgtcgaag tgaagatcgc cgacaacagc
     2221 gaaatactgg tcaagggtcc gatgctgctc aaggaatatt acaagcgacc tgaagcgacg
     2281 gccgagtcga tcaacgacga ggggtatttc atgaccggcg atgcgggcct gattgacgag
     2341 gagggccatc tcaggatcat tgaccgtgcc aaggacgtcg gcaagctggc caatggagcg
     2401 atgtacgcac ccaactacat cgagaacaag ctgaaattct tccagcacat caaggaagcg
     2461 gtggttttcg gcgatcagcg caagatggtc tgcgcgttca tcaacatcga catcggcgcg
     2521 gttggcaact gggccgagcg acgcggcatc gcctattcgg ggtataccga tctggcggcc
     2581 aagccggaag tctatcaact gattcaggac tgtatcgaga aggtcaacgg cgaactggtc
     2641 ggcgaaggtg ctctggccga ttcgcagatt caccgctttc tgatcctgca caaggagctg
     2701 gacccggacg atgacgaact gacccggacg cgcaaggtgc gccgcgactt cgtcgcccaa
     2761 aaatatgccg ttctgatcga tgcactctac agcgggaaat ccagccagtt cattgaaacg
     2821 gcggtcaagt tcgaggatgg cagacagggc aaggtggcgg ccgacctgct gatccgggat
     2881 atcaagacct tcggcattgc cgggagggcc gcctgatgag tcggcagatc ggtgaagtca
     2941 ttctcgacct gaaaggcgtt tcgctggctt tcggcggcgt caaggcattg agcgatatct
     3001 ccttcgatgt gcgcgaacac gagatccgcg ccatcatcgg cccgaatggc gccggcaaga
     3061 gctcgatgct caatgtgatc aacggtgtct atcggccgca gcaaggcgaa atcatcctgc
     3121 gcggcgagca tttcgcccgc atgaacccgt acaaggccgc caaagccggt atttcgcgca
     3181 cctttcagaa catcgccctg ttcaagggaa tgaacgtgat cgacaacatg atgaccggtc
     3241 gcaacctgaa gatcaggagc aatctgatcc agcaggcatt ctggtggggt ccggcccggc
     3301 gcgaggagct tgcccatcgc gccaaggtcg aggaggtgat cgacttcctc gaaatccagc
     3361 acattcgcaa gacgccggta gggcgcctgc cgtatggcct gcagaagcgc gtcgacctcg
     3421 gtcgcgccct ggccatggag ccgagcgtcc tgctgcttga cgaaccgatg gccggaatga
     3481 acgtcgaaga aaagcaggac atgtgtcgtt tcattctcga cataaatgac cagttcggta
     3541 cgaccatcgt attgatcgaa cacgacatgg cggtggtcat ggatatctcc gaccgcgtcg
     3601 tggtgctcga ctatgggaag aagatcggtg atggcgatcc ggacgaagtc cgcgccagcc
     3661 ctgaagtcat cagcgcctat cttggcgtca gccactgagg agtgagtcca tgggtttctt
     3721 ccttgaaact ttgatcggtg gcttgatggc cggcatgctc tattcgctga tcgcgctggg
     3781 ctttgtgctg atttacaagg cctccggcgt attcaatttt gcgcagggag cgatggtgct
     3841 gttcgcggca ctggccatgg ccaggtttgc cgaatggctg ccgggctggc ttggccttga
     3901 gagtctgtgg ctggccaaca ccatcgcttt tgtcgtcgcc gcggccttga tgtttgtcgt
     3961 cgcctggctg atcgaacgcc tggttttgcg ttatctggtg aatcaggagg gcgcgacatt
     4021 gctgatggcc acactgggta tcgcttatgt cctcgagggg gtcggctcca gcctgttcgg
     4081 caacgacgtc tacaggatcg acgtcggcat gcccaaggac ccggtgctca ttctggacgg
     4141 catcttcgag ggcggcttgc tgatcaacaa ggaggatttc atcacggcgc tgatcgccgc
     4201 cctgctggtc gggctgttga cgatcttctt ccagaaaact accactggcc gggccttgcg
     4261 cgccgtggcg gacgaccatc aggcagccca gtcgatcggc atcccgctca accacatctg
     4321 ggtaatcatc tggtccgtcg ccggtctggt cgcactggtc gccggtgtga tctggggttc
     4381 caagctcggt gtccaattct ctttggcgac ggtcgcgctg cgcggcttgc cggtcgtcat
     4441 tctcggtgga ttgacctcga tccctggggc gatcatcggt gggttgatca tcggtgtggg
     4501 cgaaaagcta tccgaggtct atcttggtcc gctgatcggc ggcggcatcg agatctggtt
     4561 cgcctaccaa ttggcactgg ttttcttgct gttcagaccg caaggtctgt ttggcgaaaa
     4621 gatcatcgac cgcgtgtaat cgaggagaac gcaagtgctc tacagggaaa acggccagtt
     4681 caagaccacc tacgcggcgg atcagcagct ctttccgatt gatcaggacc gctggtccat
     4741 tctcggtgtc gtgctgtttg ccttcatcgc cgttccactg ctggtcgacg agtacatgtt
     4801 ccgcgccatt ctcatcccct tcctgattct ttcgctggct gccgtcgggg tcaatatcct
     4861 ggttggctat tgcgggcaga tcacgctggg cgccggagcg ttcatggctg tcggcgctta
     4921 cgcggcctac aatttccata tccgaatcga tggcctgcca ttgatcgtat ccatcctgct
     4981 cggcggcctt acttcggcac tggtcgggat atttttcggg attcccagct tgcgggtgcg
     5041 cgggctctac ctggcggttg ccaccctggc agcacagttc ttcactgact gggtattctt
     5101 gcgcattccc tggtttacag acaattcatc ttccggctcg gtttcggttt ccaacttgca
     5161 ggtgatgggc tggcagatca acaacccggt aagcaaatac ctgttttgcc tgtttttcct
     5221 gacggtcttc gcgctgctgg caaagaacat gacgcgctgc gccatcggtc ggcagtggat
     5281 ggcgatacgc gatatggatg tcgccgccga agtgatcggc atccggccac tggttgccaa
     5341 gctttcggct tttgccgtca gttcgttttt tgtcggaacc gccggcgcgc tatggggttt
     5401 cgtccatctg gcatcgtggg aaccggcagc cttttcagtc gatcgctcgt tccagttgct
     5461 gttcatggtc attatcgggg gcctcggaac aatcatgggc agcttcttcg gcgccgcctt
     5521 catcgtcatt ttgccgatcg cattgaatca gctactaccg gcgttcggtg gattgatcgg
     5581 cgtcgaaata tccacggcgg gtgtgtcgca tgccgagttg gttatctttg gcggcctgat
     5641 cgtcttcttc ctgatcaagg agccgcacgg catcgcacgc ctgtggtcga ccggcaagga
     5701 gaagttgcgg ctctggcctt tcccgcattg agtcgcgtct ttcggttgtt gacctgtctt
     5761 tcgtagttgc agcagcagca ccaccagcac cacaagtacc accatttact ataagaggag
     5821 acacaaatga acctacgcaa gctcgcatta gctgcttcac taagcgctgt cgttctgtcg
     5881 tcgctggcca cagcgccggc gaacgccgca gccaaggagc aattttttcc agccctggta
     5941 tatcggactg gcgcctatgc agccaacggt gcgccatggg ccaatggcta tctcgactac
     6001 ctgaagttgg tgaatgcgaa gggtggcatc aacggcgtca aggtcgtttt cgaggaatgt
     6061 gaaacgggtt acgccaccga ccgcggcgtc gaatgctacg agcgtctgaa aggcaaacac
     6121 ggcggcgcta cggtaatcca gcctctgtcg accggcatca ctttcgcgct gacggaaaaa
     6181 gcgccgaaag acaagatccc gctgatcacg gccggttacg ggcgtagcga gtcgtcgaac
     6241 ggtgccgtct ttacctggaa cttcccgttg ctcggaacct actgggtcgg tgccgatgtc
     6301 gcgattcagt atatcgccca gaaggaaggg ggcatcgcca agctgaaagg caagaagatc
     6361 gcccttgtgt accacgacag cccatacggc aaagagccta tcgcgctgct tgaggaactg
     6421 tccaagaagc atggtttcga cgtgcagttg ctgcctgtgg cgcatccagg cgttgagcag
     6481 aaagcaacct ggctgcagat ccgtcagggc aggccggact acgttctgct ctggggttgg
     6541 ggcgtgatga actcgacggc gctgcgcgag gcccaggcga ccggctatcc gcgcaacaag
     6601 atgctgggcg tctggtggtc gggagcagat cccgatgtca aggacgttgg cgaaggtgcc
     6661 aagggctaca gtgcgctgac cctgcaggac ggtgccgatt ccaattccaa gatcgtcaag
     6721 gatatcctcg ccgcagtaca tgccaagggt gacggcaccg gaccgaagga cgaagtcgga
     6781 tccattctgt acatgcgcgg gttgatctcg gcgatgctcg gcgtcgaagg tgtccgcaag
     6841 gcgcaggagc gctatggcaa aggcaaagtg atgaccggcg agcaggtgcg ctggggcctt
     6901 gagaacctta acctcgatca ggcgacactg gatgcgatgg ggttcgctgg cgtgatgcgt
     6961 ccggtgcaga cctcgtgcct cgaccacatg ggctcgtcct gggtgcgcat ccggacatgg
     7021 aatggaagta aatgggagac ctcttccgac tggttccagg ccgacgaaaa gacgctgcgt
     7081 ccgatggtga tgctggcctc cggcaagtat gccgacgaca agaagctgac gccgcgcacg
     7141 cctgaagaat gcaagatgta gttactgtag ccgtaatgtt tcccggcgga ctcgctacgg
     7201 cgagtccatt gtccgccctc ccgcgtgcgg gagggcaatg ctgcacgttt cgcgaagaga
     7261 cgccatgagc gacgccaaca tcattctcag agtcaacagc atcgaggtga tctacaacca
     7321 cgtgatcctc gtgctcaaag gcgtttcctt cagcgtcccc gaaggaggca tcgtggccct
     7381 cttgggcggc aatggcgccg gcaagacgac gacattgcgc gcggtcagca atctgctgcg
     7441 cggcgaacgc ggcgaggtca ccaagggaac catcgagttg cgcgacgagc ggatcgaggc
     7501 gctcactccg gcgcaactgg tcaagcgcgg agtcatccag gtcatggaag gtcgacattg
     7561 cttcgcgcat ctgagcattg aagacaacct gctgaccggt acttatacga ggaccgacgg
     7621 caaggcggcg attgccgaga cgctcgacaa ggtttacacc tattttccac gcctcaagac
     7681 tcgccgcagc agccaggcgg cctacacctc ggg
//
LOCUS       DAORPM010000102         7691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2368_length_7691_cov_11.125982, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000102 DAORPM010000000
VERSION     DAORPM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2368_length_7691_cov_11.125982"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PLD53_06430"
     CDS             complement(<1..1154)
                     /locus_tag="PLD53_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRC60583.1"
                     /translation="MKITYTTSVLTALAAIFLPVAGALAADGNVGDEVPLPTGQTTVA
                     QGTWLGGDYLTGDWGGKRTELADKGYTFFAYLNGIVAKNVSGGIKTGSSSATDLYAGM
                     TVDLQKSMGWAGWTFNLSGINRAGSSIDDDVGGIYSVMQLVGGQTYFLYNVSLEKAWD
                     NGKHALKFGRITATDDFVGSPFYGYYLSNSIDGQIRAVLFDGVMTSYPFAVWGARYQY
                     TPNDGFRAKLGVYQLTENMWDPDYHGTDFAFRSSDGVSVMGQLEWNWKSAGKPGHFNL
                     GMNAVSFAMPDFDSDSTTKPFYRYYAQIDQQVTARSEGSERGLYLFGTTAYTNQQKPA
                     LVPFQVSLGAQYVGPFEGRSQDRAIFGTTYGKLSDDYAAEQQSLGNGDPT"
     gene            complement(1273..3042)
                     /locus_tag="PLD53_06435"
     CDS             complement(1273..3042)
                     /locus_tag="PLD53_06435"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HRC60584.1"
                     /translation="MAKVINIVAVTACPTGIAHTYMAAEALKKTAALMGHSISVETQG
                     AAGAKDTLSDEALAAADLVILAADTHVELTRFEGKAVYETSTSTAIRDTQGVLDAALA
                     LIGIAPAAKPAATAAAEAAPKVPQTKKIVAITACPTGIAHTFMAAEALKKAAAARGYE
                     IKVETQGSVGAKNQLSAEEIAAADVVIIGADTHVDKSRFGGKAVYETSVGRALKETDK
                     VMQEAFAVQPQAGTDVPQEVKKAKASPTEKRAGPYKHLLTGVSYMLPIVVAGGLLIAL
                     SFVFGIEAFKEKGTLAASLMDIGGGAAFALMIPVLAGYIAFSIADRPGLAPGLIGGML
                     ASQLQAGFLGGIVAGFVAGYVALAMRKHIKVPTHFEGLMPVLVIPLLATLVVGLLMVY
                     VVGGPAKAIMDGLTHFLQGMGSTNAVILGLLLGGMMAVDMGGPTNKAAYTFAVGLLAS
                     NSFLPMAAVMAAGMVPPLGIALATFIAKNRFSADEREAGKAAAVLGISFITEGAIPFA
                     AKDPLRVIPSCVVGAALTGALSMLFGCTLRAPHGGVFVLAIPNAVGNLLPYIGAIVAG
                     TIVTAVMLMLLKKPIVDGPAVAA"
     gene            complement(3084..4037)
                     /gene="pfkB"
                     /locus_tag="PLD53_06440"
     CDS             complement(3084..4037)
                     /gene="pfkB"
                     /locus_tag="PLD53_06440"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HRC60585.1"
                     /translation="MITQSIGIATVTLNPAIDQSVAIANFAAGEVNRVDWEQSDPGGK
                     GVNVASFLADLGCRVAVTGLLGNENALAFERLFAQKGMTDAFVRIPGKTRVNVKIIDA
                     AQQRITDINFPGQAASPADVAGLRDQLAALIDRHEWFVLSGSVPTGIPVGLYGEWVRM
                     LKSAGKRVLLDTSGEPLRASIGEAPFAIKPNIAELEELVGQRLPTEAAVVDAARALLS
                     KGIQCVVVSMGKQGALFVDADECLLAIPPTVDVQSTVGAGDAMVAGFVAAKIRGCSLA
                     ECARLATATAAGALTQLGPRLPPAQTIESLMQQVSLRAPAQ"
     gene            complement(4052..6523)
                     /gene="ptsP"
                     /locus_tag="PLD53_06445"
     CDS             complement(4052..6523)
                     /gene="ptsP"
                     /locus_tag="PLD53_06445"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRC60586.1"
                     /translation="MIKLGKESIRLNAQASNKTEAIRIVGSLLVDSGNMKAGYIESML
                     LREKVANTYLGNGIAIPHGLPKDRDLILDTGIAVAQFPAGVQWNSGETVHLVVGIAAR
                     SDEHIELLANLTDVLDDEQTVRRLASTEDPMDIIARLTQPREEPSALATAAAVQADDF
                     AKWVDVALGSGAGLHARPATAFVEIAKQFQSEIRVRHVSKLANGKSLVSLLKLGADER
                     ASIRIMARGVDEDAALQALAAAVRNGLEDEDESGHAAPEAGSGPILTLSGNAIAGISA
                     SPGLAIGPLRHFRREKIVVAVTANDPAREEIRLRQSIEAAKVQLRELHDEVKERSGAA
                     RAAIFRAHEEFLDDPDLVDTALERIKGGHSAGWSWRETFEERARDVANLGDALLRERA
                     VDLQDVGRRVLRLLADTLEEEPGVLDSPVILVAEDLSPSDTAKLDPAMVLGLCTAAGG
                     PTSHTAIIARSLDIPAIVGAGPAILNQPEGGLCVLDGDSGNLYCEPSEADLALARAAQ
                     EDWQARREAERLACYQPAITTDGRRIEVVGNIGSAAEAQQAVDAGAEGVGLLRTEFLF
                     LERSAPPTEEEQFETYRAMTKALNGLPLIIRTLDIGGDKEVPYLSLPAEQNPFLGVRG
                     IRLCLARLDLFKPQLRAIYRAAETGPVKIMFPMIATLEELITARAIAEEVRRELHARP
                     VEIGIMIEVPSAVLMATELAAHADFFSVGTNDLTQYVLAMDRLHPSLARQADALHPAV
                     LRMIDMTVKAATAAGRWVGVCGGIAGDPKGAAILAGLGVTELSMSIPSVAAVKARIRK
                     LSFADVEALAQRALACKTAAEVRAL"
     gene            complement(6659..7441)
                     /locus_tag="PLD53_06450"
     CDS             complement(6659..7441)
                     /locus_tag="PLD53_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRC60587.1"
                     /translation="MAEHLLDHRLQLILSVLSTRGECRTRQLAEQFQLSEMTVRRDLQ
                     ELESRGQLRRVHGGAVLLNRDVNYSLRLEHDQAQKQFIGQAATRFLKSGQTVYIDAGT
                     TALALARAIRQGLHQVTHLHIVTHGIPIATELAGNTPYSVQLIGGDVYQNALSTVGPT
                     ALAQISGLDIDLFFMGAGGVDASMGWSNSNHVEAEIKRTVIKRSKATCVIADGAKWGQ
                     QSFAPIVPLGEVSRWIVDRGLIAEGAAAAAAAGVAVTFADAL"
BASE COUNT         1340 a         2454 c         2469 g         1428 t
ORIGIN      
        1 taggtcggat cgccgttgcc cagggactgc tgctcggctg catagtcgtc gcttagcttg
       61 ccgtaggtgg tgccgaaaat cgcgcggtcc tgactgcgcc cttcaaacgg cccgacatac
      121 tgggcgccaa ggctgacctg gaagggaacc agggccggct tttgctggtt cgtgtaggcc
      181 gtggtgccga acaggtagag cccgcgctcg gaaccctcag acctggcggt cacctgttgg
      241 tcgatctggg cgtagtagcg gtagaagggc ttggtcgtac tatcggagtc gaagtccggc
      301 atggcgaagg acacggcgtt catgcccagg ttgaaatggc caggcttgcc cgcggacttc
      361 cagttccact cgagctggcc catgaccgag accccgtcgc tggaacgaaa cgcaaaatcg
      421 gtgccgtggt agtccgggtc ccacatgttt tcggtgagct ggtagacacc gagtttggcg
      481 cggaagccgt cgttgggtgt gtactggtat ctggcacccc aaacggcaaa ggggtaggaa
      541 gtcattacgc cgtcgaacag cacagcgcga atctggccgt cgatactgtt cgacaggtag
      601 tagccataga agggcgaacc gacgaagtcg tctgtagccg taatgcgacc gaacttcaag
      661 gcgtgcttgc cgttgtccca ggctttttcc aggctgacgt tgtagaggaa atacgtctgg
      721 ccaccgacca gctgcatgac gctgtagata ccgccgacat cgtcgtcgat gctcgacccg
      781 gcgcggttga tgccgctgag attgaaggtc cagccggccc agcccatgct cttctgcagg
      841 tcgaccgtca tgccggcata gagatcggtc gccgacgagc tccccgtttt gatgccacca
      901 ctgacgtttt tcgcgacaat accgttgaga taggcaaaga aggtgtaacc cttgtcggcg
      961 agctcggtgc gttttcctcc ccagtcgccg gtcaggtaat ctccgccaag ccacgtgccc
     1021 tgggcgaccg tggtctggcc agttggcagc gggacctcgt cgccgacgtt accgtccgct
     1081 gccaaggcgc cagcgacggg aagaaagatg gccgccaggg ccgtcaggac ggaagtggtg
     1141 taggttattt tcatgtttgt gccctcggta ttgagtcaag atttccgtag attcgggccg
     1201 cagattcccc tgcctttccc gataagccaa gaaacggtgc ggcgggtcgg gacatggccc
     1261 gacccgggtg ggttacgcgg cgacggccgg gccgtcgacg atcggctttt tcagcagcat
     1321 caacatcacc gcagtaacga tcgtgccagc aacgatggcg ccgatatagg gcagcagatt
     1381 gccgaccgca ttgggaatcg cgagcacgaa gacgccgccg tgcggggcgc gcagggtgca
     1441 cccgaagagc atcgacagcg ccccggtcag cgccgcgccg accacacacg aaggaatcac
     1501 gcgcaagggg tccttcgccg caaaggggat ggcgccttcg gtgatgaacg agataccgag
     1561 gaccgcggcg gccttgccgg cttcccgttc atcggccgag aagcggttct tggcgatgaa
     1621 cgtcgccagg gcgatgccca gtggcggcac catgccggca gccatcaccg cggccatcgg
     1681 caggaagctg ttgctcgcca gcaggccgac ggcgaaggta taggccgcct tgttggtcgg
     1741 cccgcccatg tccaccgcca tcatgccgcc caacagcagg ccaaggatga ccgcattggt
     1801 cgagcccatg ccctgcagga agtgggtgag accatccatg atcgccttgg ccggcccgcc
     1861 gacgacgtag accatcagca gtccgacgac cagggtcgcg agcaacggaa tgaccagcac
     1921 gggcatcagt ccctcgaaat gggtcggcac cttgatgtgc ttgcgcatgg ccagcgccac
     1981 gtaaccggcc acgaatccgg cgacgatgcc accgagaaag ccggcctgga gttgactggc
     2041 cagcatgccg ccgatcagac cgggtgccag ccccggccgg tcggcgatgg aaaacgcgat
     2101 gtagccggcg agcacgggaa tcatcagggc gaaggcggca ccaccgccga tgtccatcag
     2161 gctggccgcc agcgtcccct tctccttgaa ggcctcgata ccgaagacga acgacagggc
     2221 gatcagcaaa ccgccggcga cgacgatcgg gagcatgtag gagacaccgg tgagcaggtg
     2281 cttgtacggg ccggcgcgtt tttccgtcgg gctcgccttg gccttcttga cctcctgcgg
     2341 cacgtcggtg ccggcttggg gctgcacggc aaacgcctcc tgcatcacct tgtcggtctc
     2401 cttcaaggcc cggccgaccg aggtttcata aacggccttg ccgccgaaac gcgatttatc
     2461 cacgtgcgta tcggcgccga tgatgaccac gtcggcggcg gcaatttctt ccgccgacag
     2521 ttgattcttc gcgccgaccg agccctgcgt ttccaccttg atctcgtagc cgcgggcggc
     2581 agccgctttc ttcaaggcct ctgccgccat gaaggtatgc gcgatgccgg tcggacaggc
     2641 ggtgatggcg acgatctttt tcgtctgtgg cacctttggt gccgcctcgg cagccgccgt
     2701 cgccgccggc tttgccgcgg gcgcgatgcc aatcagggcc agggcggcgt cgagtacgcc
     2761 ttgcgtgtca cggatagcgg tactggtgct ggtttcatag actgccttgc cttcgaagcg
     2821 cgtcagctcg acgtgggtat cagcggcgag aatgaccaga tcagcggctg ccagcgcctc
     2881 atcgctgagc gtatccttgg cgcccgccgc gccctgcgtt tcgacactga tgctgtggcc
     2941 catcagcgcg gcagttttct tcaaagcttc ggcggccatg taggtatggg cgatgccggt
     3001 cggacatgcg gtgacggcga cgatgtttat gacttttgcc atttcggttt ctcctctgga
     3061 atttaccggg ctcgtgttcg cgctcattgc gccggggccc gcaagctgac ctgctgcatc
     3121 agggactcaa tggtctgcgc cggcggcagg cgcggaccga gctgcgtcag cgcgccggcg
     3181 gcggtcgccg tggccaggcg tgcgcactcc gccagcgagc agccgcgaat ctttgcagcg
     3241 acgaagccgg cgaccatggc gtcgcccgcg ccgacggtgc tctgcacgtc gaccgtgggc
     3301 gggatggcca gcaggcattc gtcggcatcg acgaacaagg cgccctgctt gcccatcgag
     3361 accacgacac actggatacc cttgctcagc agagcgcgtg ccgcatctac caccgcggct
     3421 tcggttggca agcgctggcc gacgagctcc tcaagttcgg cgatattcgg cttgatggca
     3481 aacggcgctt cgccgatcga agcacgcagc ggctcgccgc tggtatcgag cagcacgcgc
     3541 ttgccggcgc tcttcagcat cctcacccat tcgccgtaga gtcccaccgg gatcccggtg
     3601 ggaacgcttc ccgagagtac gaaccactcg tgacggtcga tcaaggcggc gagttggtca
     3661 cgcagcccgg cgacatcggc tgggcttgcc gcttgccccg ggaagttgat atcggtgatg
     3721 cgttgttgtg ccgcatcgat gatcttgacg ttgacccgcg tcttgccggg gatgcgaaca
     3781 aaggcgtcgg tcatgccctt ctgggcgaat agccgctcga aggcgagcgc gttctcgttg
     3841 ccgagcaggc cggtcacggc gacgcgacag cccaggtcgg cgaggaagga ggcgacattg
     3901 acgcccttgc cgccggggtc ggactgctcc cagtcgacgc ggttgacctc gccagcggcg
     3961 aaattggcaa tggcgacgct ctggtcgatg gccggattca gcgtcaccgt cgcgatgccg
     4021 attgattgag tgatcatggt ttctcctttg cttacagggc gcgtacttca gccgctgtct
     4081 tgcaggccag cgcgcgctgc gcgagagcct cgacgtcggc gaaagaaagc ttgcgaatcc
     4141 tggccttgac ggcggccacg ctggggatgc tcatgctcag ctcggtgaca cccagaccgg
     4201 ccagaatcgc cgcacccttg gggtcgccgg cgatgccgcc gcagacgcca acccaccggc
     4261 cggccgccgt ggccgccttc acggtcatgt cgatcatgcg caggaccgcc gggtgcaaag
     4321 cgtcggcctg gcgcgccagg ctcgggtgca gccggtccat ggccagcacg tactgggtca
     4381 ggtcgttagt gcccaccgaa aagaagtcgg catgcgcggc gagttcggta gccatcagga
     4441 ccgccgacgg gacctcgatc atgatgccga tttcgaccgg cctggcatgg agttcccggc
     4501 ggacttcctc ggcgatcgcg cgggccgtga tcagctcctc gagcgtggcg atcatcggga
     4561 acatgatctt caccgggccg gtttcggcgg cacgatagat ggcgcgcagt tgcggcttga
     4621 agagatcgag ccgggccagg cacaagcgga tgccgcgcac gccaaggaag gggttttgct
     4681 cggcgggcag cgacaggtac ggcacttcct tgtcgccgcc gatgtcgagc gtgcgaatga
     4741 tcagcggcag gccattgagc gccttggtca tcgcccggta ggtctcgaac tgctcctcct
     4801 cggtgggcgg cgccgagcgc tccagaaaca ggaattcggt acgtagcagt ccgaccccct
     4861 cggctccggc atcgaccgcc tgttgcgctt ccgccgccga accgatatta ccgaccactt
     4921 cgatgcgccg gccgtcggtg gtgatcgccg gctggtagca ggcgaggcgc tcggcttcgc
     4981 ggcgcgcctg ccagtcctct tgagcggctc gcgccagtgc cagatcggcc tcgctcggct
     5041 cgcaatagag attgcccgag tcgccgtcaa ggacgcacag cccaccctcg ggctgattga
     5101 gaatcgccgg gccggcgccg acgatcgccg gaatatcaag cgagcgggct atgatcgccg
     5161 tgtgcgacgt cggcccgccg gcggcggtgc acagaccaag caccattgcc gggtcgagct
     5221 tggcggtgtc cgatggcgac agatcctcgg ccaccaggat gaccggcgag tcgagcacac
     5281 cgggctcttc ctccagcgta tcggccaaca ggcgcaatac ccgtcgcccg acgtcctgga
     5341 ggtcgacggc gcgctccctg agcagcgcat cgcccagatt ggcgacgtcc cgcgcgcgct
     5401 cttcgaacgt ctcgcgccac gaccagccgg cgctgtgccc gccctttatc cgctcgagcg
     5461 cggtatcgac cagatccggg tcgtcgagaa actcctcgtg tgcccggaag atggctgccc
     5521 gggcggcgcc gcttctttcc ttgacttcgt cgtggagttc gcgcaactgc accttggctg
     5581 cctcgatcga ctgccgcaag cggatttcct cgcgcgccgg atcgttggcg gtgacggcga
     5641 cgacgatctt ttcgcgccgg aaatggcgca agggtccgat agccagacct ggcgaggcag
     5701 aaatcccggc gatcgcgttg ccggagagcg tcaggatggg accgctgccg gcttcgggcg
     5761 cagcgtgacc gctctcatcc tcgtcttcga gaccgtttcg cacggccgcc gccaaggcct
     5821 gcaatgcggc gtcctcgtcg acgccacggg ccatgatgcg gatcgaggcg cgctcgtcgg
     5881 cgcccagctt caacagggag accagactct tgccgttggc cagcttgctt acgtggcgga
     5941 cgcgtatttc cgactggaat tgcttggcga tctcgacgaa ggccgtggcc ggcctggcgt
     6001 gcagtccagc gccgctgccg agcgcgacat cgacccactt ggcgaagtcg tccgcctgca
     6061 ccgccgctgc agtggcgagt gccgacggct cctcccgcgg ctgcgtgagg cgcgcaatga
     6121 tatccattgg atcttcggtg ctggccaggc gtcggactgt ctgctcgtca tcgagaacat
     6181 cagtcaggtt ggccagcaac tcgatgtgct cgtcggagcg tgcggcaata cccactacca
     6241 gatgcaccgt ctcgccagaa ttccactgta cgccggcggg aaactgcgcc acggcaatgc
     6301 cggtgtcgag aatcagatcc cggtccttgg gcaagccgtg gggaatggca atgccgttgc
     6361 caagataggt gttggccacc ttctcgcgca gcagcatgct ctcgatgtac cctgctttca
     6421 tgttgccgct gtccaccagc agactgccga cgatgcgaat cgcctccgtc ttgttgctgg
     6481 cttgcgcgtt cagcctgatg ctttccttgc cgagcttgat catgtcgttc ctccttgtgt
     6541 gttgaacact tgaaatacgc cccgatgccc gtctcgccgc cgtgttatcg aggtcgctgg
     6601 aatcgcgcgg cgaatgctgt acctacctgc tgctaccgac cacacaccat cccgttgctc
     6661 acagagcatc ggcaaaggtg actgccacgc cggcagcggc cgcggcggcg gcgccttccg
     6721 cgatcagtcc gcgatcgacg atccagcgcg acacttcgcc caacggcacg atcggggcaa
     6781 acgactgctg gccccacttg gcgccatcgg cgatgacgca tgtggctttg ctgcgcttga
     6841 tgaccgtgcg cttgatctcg gcctcgacat gattggagtt actccaaccc atgctggcgt
     6901 cgacgccacc ggcgcccatg aagaagagat cgatatcgag acccgagatc tgtgccaacg
     6961 cggtcggacc gacggtgctg agcgcgttct gatagacgtc gccaccgatc agctgcacgc
     7021 tgtacggggt gttaccggcg agctcggtag cgatggggat gccgtgggtg acgatgtgca
     7081 gatgggtgac ttgatgcagc ccctggcgaa tggcgcgcgc cagcgccagc gcggtggtac
     7141 cggcatcgat gtagacggtt tggccgctct tcaggaagcg cgtcgcggcc tggccgatga
     7201 actgcttctg cgcctggtca tgctccaggc gcagggagta attgacgtca cgattgagca
     7261 atacagcccc gccatgcacg cgccgcaact ggccacgcga ctcgagctcc tgcaaatcgc
     7321 ggcgtaccgt catctcggat aactgaaact gctctgcgag ctgccgcgtg cggcactcgc
     7381 cgcgcgtcga caacaccgac aggatcaact gcagtcgatg gtccagcagg tgttctgcca
     7441 ttcttacttc ctttccttgt tgtgtcgttg cacaccctgt tttgcgacga tgattggtca
     7501 tcgggaaccc ttggaaagcg agactgaacg ggcgtaaatg tgcgaattgt ttgcttaacg
     7561 atcataatcg cacaatatcg cacattgcgc aagcgctatt tcgccaacac aacactcgaa
     7621 cgcctccgtt gcacgtgagc tgactgtcgg aagattccca cgtagcgacc gaccgcagaa
     7681 ctattgataa c
//
LOCUS       DAORPM010000103         7572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2449_length_7572_cov_9.637488, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000103 DAORPM010000000
VERSION     DAORPM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2449_length_7572_cov_9.637488"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLD53_06455"
     CDS             complement(<1..223)
                     /locus_tag="PLD53_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRC60588.1"
                     /translation="MKTRCPVCQTTFRVTPEQLKVRAGNVRCGQCHAVFNALDGLLDE
                     VGPSAPSSQQPESTETFFDDAFLSQDTPDN"
     gene            complement(227..1117)
                     /gene="prmA"
                     /locus_tag="PLD53_06460"
     CDS             complement(227..1117)
                     /gene="prmA"
                     /locus_tag="PLD53_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRC60589.1"
                     /translation="MAWLSVSIETDCSHADALADALIAAGALSASIEDADAGTPNETP
                     QFGEPGSLTTPGWERSRIVALLEPDANVGDLVADCARIAGFEITPAFTQKSIAEQDWV
                     TLTQSQFDPIRVSDGLWIVPSWHQAPDPSAIILVLDPGMAFGTGSHPTTRLCLEWLEN
                     SVTPGVSVLDYGCGSGILALAASKLGASKVVGVDIDLQAVSAAKSNAERNEVSARFVD
                     SAEEIRGQFDIVVANILSNPLMALAPAICSHVCPGGKLALSGILAEQAEKLIASYAPY
                     LQLDVADTRDGWVCLTGINI"
     gene            complement(1129..2493)
                     /gene="accC"
                     /locus_tag="PLD53_06465"
     CDS             complement(1129..2493)
                     /gene="accC"
                     /locus_tag="PLD53_06465"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRC60590.1"
                     /translation="MFGKVLIANRGEIALRIQRACRELGIKTVVIHSEADREAKYVKL
                     ADESVCIGPPPSNLSYLNVPAIISAAEVTDSEAIHPGYGFLSENADFAERVESSGFVF
                     IGPRPETIRLMGDKVSAKDAMKAAGVPCVPGSEGALPDDSKEIVKIAKAIGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVVMTRAEAQNFFGNPNVYMEKFLENPRHVEIQVLADS
                     FKNAVYLGERDCSMQRRHQKIIEEAPAPGIPPRHVARIGERCAEACRRINYRGAGTFE
                     FLYENGEFYFIEMNTRIQVEHPVSEMISGVDLVQEQIQVAAGKKLRFKQRDLSFRGHA
                     IECRINAEDPFTFVPFPGKITTYHPPGGPGIRVDSHIYQGYTVPQHYDSMVAKVIAYG
                     DTRDQAIRRMRIALSEMSIEGIKTNIALHQELMLDAGFIKGGMNIHYLEKKLAAKGEV
                     KKND"
     gene            complement(2509..2964)
                     /gene="accB"
                     /locus_tag="PLD53_06470"
     CDS             complement(2509..2964)
                     /gene="accB"
                     /locus_tag="PLD53_06470"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRC60591.1"
                     /translation="MDLRKLKKLIDLVEESGITELEVTEGEERVRIAKHSTAAPQQAY
                     TMPQATTVALPAAGPAIDAPADSKALNLPEGHVIKSPMVGTFYRSASPDSDPFVEVGS
                     IVKSGDTLCIVEAMKLLNEIESDCAGTVKAILVENGQPVEYGEPLFIVA"
     gene            complement(3042..3533)
                     /gene="aroQ"
                     /locus_tag="PLD53_06475"
     CDS             complement(3042..3533)
                     /gene="aroQ"
                     /locus_tag="PLD53_06475"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRC60592.1"
                     /translation="MKKEKATSSDNKKSGEPVKLLVLHGPNLNLLGTREPNYYGLATL
                     SDINLALARRAAAVGAELESFQSNHEGALIERVHAAREQRVRYIILNPAAYTHTSIAL
                     RDAMAASGIPFVEVHLSNVHSRETFRHHSYFSDLAVGVITGLGSEGYLLALEYLLSQI
                     NAD"
     gene            complement(3579..>3962)
                     /locus_tag="PLD53_06480"
     CDS             complement(3579..>3962)
                     /locus_tag="PLD53_06480"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRC60593.1"
                     /translation="KTLAMSQWRGRTLVVNFWATWCPPCKEEMPAFSRLQTQYAANGV
                     QFVGIALDSADNVLNFSKQHPVTYPLLLAGSEGAELSRQLGNSLLTVPYTVVLGANGG
                     VHTTRMGPFPKQELEALLQKIAIAK"
     assembly_gap    3963..4062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4063..4459)
                     /locus_tag="PLD53_06485"
     CDS             complement(<4063..4459)
                     /locus_tag="PLD53_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60594.1"
                     /translation="MNKAQQRKRIASVAARLVAEHGIGDYALAKRKAAHGLGLPEDTQ
                     LPDNAELEMELSTYQRLFHADEHTERIADLLRIASETMLIMQKFNPYLTGSVLDGTAG
                     RYAEIDIQLYTDSAKDVEIYLLNQRISYEH"
     gene            4672..6042
                     /gene="mpl"
                     /locus_tag="PLD53_06490"
     CDS             4672..6042
                     /gene="mpl"
                     /locus_tag="PLD53_06490"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HRC60595.1"
                     /translation="MHIHILGICGTFMGGIAQLAQAAGHRVTGCDSGVYPPMSTQLEA
                     SGIELVEGYGIEQLALKPDFFLVGNAISRGNPLLEEILDRGLRYVSGPQWLSDNVLQG
                     RWVLAVAGTHGKTTTSAMLAWILEDAGLSPGFLIGGVPMNFGVSARLSGNVGESPFFV
                     IEADEYDTAFCDKRSKFIHYRPRTAILNNLEYDHADIFADLASIETQFHHLVRTLPRN
                     GLLVSNGGESSLERVLARGCWTPIERFNQAFDWRMEGDDADGSLRLMHGEEVIGKANW
                     SLNGEHNRSNAVSALLAARHVGVPFDISLEALSRFKNVRRRLELRGTVRGVNVLDDFA
                     HHPTAIATTVSGLRRRIGSGAEAPRILAVLEPRSNTMKLGVMKAQLPASLADADLVFC
                     YSAKLGWDASEALAPLGHKAQVSDDLDGLIRNIVAAARHGDQILVMSNGGFGGIHDKL
                     LAALAD"
     gene            complement(6087..6989)
                     /gene="cyoE"
                     /locus_tag="PLD53_06495"
     CDS             complement(6087..6989)
                     /gene="cyoE"
                     /locus_tag="PLD53_06495"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRC60596.1"
                     /translation="MPATFRDQANSIRTARRRISDFFGLCKPRVNSLIVFTAMIGMLL
                     ASPGFPPLTVFLSASIGIALVAGAAAAINCLVEKTVDAKMARTRWRATANGKAPMVET
                     LTLATLVGSVGLWLLHAMVNPLTMWLTLATFLGYAIIYTIVLKPATPMNIVIGGASGA
                     MPPLLGWAAMTGSVGPEALVLFLIIFIWTPPHFWALACYRREDYVKAGLPMLPVTHGI
                     ELTCFHSFLYVVMLTGATVLPYTLGMSGPWYLATVLVLDVIFLAYTFALWRNYSDALA
                     RRTFRWSIIYLTLLFAALLADHFV"
     gene            complement(7009..>7572)
                     /locus_tag="PLD53_06500"
     CDS             complement(7009..>7572)
                     /locus_tag="PLD53_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRC60597.1"
                     /translation="LLLLAAVFVLPFVVGSGLFWLDWRPEKYINHGDLLQPPIPLPET
                     GLSHADGRPLPASDFRGKWLLVLPVNGACDKDCEKSLQLMQQVHIALNKEQDRLQRVL
                     VSIDSSGTHSSPALLKLQQHFPDIVVAMAKASTEVNVWRSLLGDSGQKVFIVDPLGNV
                     MMRYAEPIDMRGVLKDLERLLKYSWVR"
BASE COUNT         1617 a         1988 c         2169 g         1698 t
ORIGIN      
        1 tattgtcagg agtgtcctgc gacagaaatg cgtcatcgaa gaacgtttct gtactctcgg
       61 gttgctgtga ggagggtgca gaagggccta cctcatcgag caaaccatcg agagcattga
      121 acacggcatg acattgaccg cagcgaacat tcccggcgcg caccttcagt tgctccgggg
      181 tgacccggaa ggtcgtttgg cagacggggc agcgagtctt cattgcctag atattgattc
      241 cagtcagaca aacccagccg tcgcgcgtat cggcgacgtc gagctgcagg taaggcgcat
      301 agctcgcgat cagtttttct gcctgttctg caagaatgcc ggagagtgcc agtttgccac
      361 cggggcacac gtgcgaacag atcgcgggcg ccagcgccat taaggggttg gagagaatat
      421 tggcaaccac gatatcaaac tgccccctga tttcctctgc tgaatcgacg aagcgagcgc
      481 tgacttcgtt acgttccgca ttgcttttcg ccgcgctgac ggcctgtaga tcaatatcga
      541 caccgactac tttactggcg ccgagtttgc ttgccgcaag ggccagaata cctgaaccgc
      601 agccgtaatc aagaaccgac acgccaggag taaccgaatt ttcaagccat tcgaggcaaa
      661 ggcgggtggt tggatgcgac ccggtgccaa aggccatgcc tgggtcgagc accagaatga
      721 tggcgctggg gtcgggtgcc tggtgccacg aggggacaat ccacaatcca tccgaaacgc
      781 gaattggatc aaactgcgat tgagtgagtg ttacccagtc ttgttcagcg atgctcttct
      841 gggtgaaggc aggcgtaatt tcgaatccgg cgatgcgggc gcagtcggcg accagatccc
      901 caacattagc atccggttca agcagggcga cgatgcgcga acgttcccat ccgggcgtgg
      961 tgagtgagcc aggctcaccg aattgtggcg tttcgttcgg tgtcccggca tcagcatcct
     1021 caatgctggc cgaaagcgcg ccggccgcaa tcagcgcgtc agccagcgca tccgcgtggg
     1081 agcagtccgt ttcgatgctg actgaaagcc aggccatttt ctgctgactc agtcgttttt
     1141 cttgacctcg cctttggcgg caagtttctt ctccaggtag tgaatgttca tcccgccctt
     1201 gatgaagccg gcatccagca tcagctcctg gtggagggcg atattcgtct ttatgccttc
     1261 gatactcatt tcagacagag caatgcgcat gcggcgaatc gcctgatcgc gcgtgtcgcc
     1321 ataggcgatc actttggcga ccatcgagtc atagtgttgc ggtacggtat agccttggta
     1381 gatgtgggag tctacgcgga tgccggggcc gccaggcggg tgataagtcg ttatctttcc
     1441 cggaaatggc acgaacgtga acggatcttc ggcgttaatg cgacattcga tcgcgtgacc
     1501 ccggaagctc aggtcgcgct gcttgaagcg tagtttcttg cctgccgcga cctgaatctg
     1561 ctcctgtacc aagtcaacac cgctgatcat ctcactcacc ggatgctcaa cctgaatgcg
     1621 cgtattcatc tcgatgaaat agaattcgcc attctcgtag aggaattcga aggtgccggc
     1681 gccacgataa ttgatgcggc ggcaggcctc ggcacaacgc tcgccgatac gtgcgacatg
     1741 tcgtggcggg atgccgggtg ctggcgcttc ttcgatgatc ttctgatggc ggcgctgcat
     1801 ggaacaatcg cgttcgccga gatagacggc attcttaaag ctgtcggcca gcacctgaat
     1861 ttccacatga cgaggatttt cgaggaactt ctccatatag acgttcggat tgccgaagaa
     1921 gttttgtgct tccgcgcggg tcatcacaac cgcattgagc aacgctgcct cggtatgcac
     1981 cacacgcatg ccgcgaccgc cgccgccgcc ggcagccttg atgattactg gatatccgat
     2041 agccttggca atcttgacaa tctccttcga atcatcgggc agcgcgccct ccgacccggg
     2101 aacgcagggt acgccggccg ctttcatggc gtccttggca gaaactttgt cgcccatgag
     2161 acggattgtt tcggggcggg ggccaatgaa aacgaagccg gaggattcga cccgttcggc
     2221 aaagtcggcg ttttccgaaa ggaagccgta ccctggatgt atggcctcgg aatcagtaac
     2281 ctcggccgcc gagatgatgg ccggtacgtt caggtagctc aggttggagg gaggaggacc
     2341 tatgcacacc gattcatcgg cgagctttac gtactttgcc tcgcggtcgg cttccgaatg
     2401 aattactacc gtcttgatac ccaattcgcg gcaggcgcgc tggatacgga gtgcgatttc
     2461 tccgcgattg gcaatgagga cttttccgaa catggcggaa ggctctcctt aagctacgat
     2521 gaacaggggc tcaccgtatt cgaccggctg gccattctcg accagaatgg ccttcaccgt
     2581 tccagcgcaa tcggattcaa tctcattgag cagtttcatt gcctcgacaa tgcacagtgt
     2641 gtcgccagat ttgactatgc tgccgacctc gacaaacgga tcggagtcag ggctggccga
     2701 gcgatagaag gtgccgacca tcggcgattt gatgacgtgt ccctcaggga gattaagtgc
     2761 tttgctgtca gcaggagcgt cgatggcggg tccagcggcc ggcaaggcca ctgttgtcgc
     2821 ttgcggcatc gtgtaagcct gttggggagc ggcagtcgag tgcttggcta tgcggactct
     2881 ttcctctcct tcggttactt caagctcggt gatacccgat tcctcgacca gatcaatgag
     2941 tttcttgagt ttacgcagat ccatgaatct aatcctttgt gaagtgatgg ttgccagtcg
     3001 caaagactgg cggcagaagt ggtcaacccg ctggcacgag gtcagtcagc gttgatctga
     3061 ctgagaaggt actcgagcgc aagcaggtaa ccctcgctgc caagccctgt aatgacgcca
     3121 accgccaagt cagaaaaata cgagtgatgg cggaaggttt cgcgagagtg aacgttggat
     3181 aggtgcacct cgacaaaggg aatgcctgac gccgccatgg cgtcccgtaa cgcgatgctg
     3241 gtatgcgtgt aagccgctgg attgaggatg atgtagcgta ccctctgttc tcgcgcagcg
     3301 tgtacgcgtt cgatcagggc gccctcgtga ttgctttgaa atgattcaag ttcggcgcct
     3361 acggctgccg cacgacgggc tagggcaaga ttgatgtccg aaagtgttgc caggccataa
     3421 tagttcggct cgcgagtgcc gagaagattc aggttgggtc catgcaggac aagtagcttg
     3481 acgggttccc cggacttctt attgtctgac gatgtggctt tttccttctt catatctgca
     3541 attttgccgc atatcgcagc attttgtcca gtaatcgctc atttcgcgat ggcgatcttc
     3601 tgcagcagtg cttcaagttc ctgcttggga aagggcccca ttctggtcgt atgcacccct
     3661 ccattggcac ccagaacgac ggtgtaaggc acagttagga gcgaattgcc caattgccgg
     3721 ctcagttctg ccccttcgga gccggcaagg agaagcgggt aggtgacagg gtgctgcttt
     3781 gagaaattga gtacgttgtc ggcagaatcg agcgcgatac ctacaaattg gacgccgttc
     3841 gccgcatatt gcgtttgcaa tctcgaaaaa gccggcattt cctctttgca gggggggcac
     3901 catgtcgccc agaagttaac caccagcgtt cttccccgcc attgagacat cgccagcgtc
     3961 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgtgctca taggaaatac
     4081 gttggttgag aagatagatt tcaacatcct tggcgctatc ggtgtatagc tggatgtcaa
     4141 tttcggcata gcgaccagcg gttccatcga gtacagagcc tgtaagataa gggttgaatt
     4201 tctgcatgat aagcatagtt tcgctggcta ttcgcaggag atcagcgatt ctctcagtgt
     4261 gctcatcagc gtgaaagagg cgttggtagg tactcaattc catctcaagc tcggcgttat
     4321 ccggtagctg ggtgtcttct ggcaggccga ggccgtgtgc tgccttgcgt ttagccagag
     4381 cgtagtcgcc gatgccatgc tcggcaacca agcgcgctgc gacactggca attcgcttgc
     4441 gttgctgagc cttgttcaag ttctcgtatc gtcggttcat ggcgctggtc gctcaggtct
     4501 cggactttgg tattcgcgct ttcttcggga atggttgcat tcctggcgcc tcgctaccgc
     4561 gcggattcgc tcgtccagcc ggattcagtc gcatcgctgc gtcaagcgta caattcgctt
     4621 ggcgcgcccg cgaacactcg tcgcacagac cattctactt ggaatttttt catgcacatt
     4681 catattctcg gcatttgtgg caccttcatg gggggaattg cacagctggc gcaagctgca
     4741 ggtcatcgcg taactggttg cgatagcggc gtctatccgc cgatgagtac tcagcttgaa
     4801 gcgtcgggca ttgaactcgt tgaaggctat ggtattgagc agcttgcgct caagccagat
     4861 ttttttcttg tgggaaacgc catttcgcga ggcaaccccc tgcttgagga gattctggac
     4921 cggggtttgc gctatgtttc cggaccgcag tggctatcgg acaacgtgtt gcaggggcgt
     4981 tgggtgttag cagtcgccgg cacgcacggc aagactacga cttccgcgat gttggcctgg
     5041 attcttgagg atgccggctt gagtcctggc tttttgatcg gtggagttcc gatgaatttc
     5101 ggcgtatctg cacgattatc gggaaatgtc ggcgaatctc cgttcttcgt gatcgaggcc
     5161 gacgaatacg acacggcgtt ttgtgacaaa cgctccaagt tcattcatta ccgaccgcga
     5221 acagcaattc tgaataatct ggaatatgac catgcggata tcttcgccga tcttgcttct
     5281 atcgagacgc aattccatca tcttgtgcgt actttgccgc gaaatggcct gctggtaagc
     5341 aacggtggag aaagtagcct cgaacgtgtg ctcgcccgcg gttgctggac gcccatcgag
     5401 cgcttcaacc aagccttcga ctggcgcatg gaaggtgacg atgcggatgg ctcgttacgc
     5461 ttgatgcatg gtgaagaagt catcggtaag gcgaattgga gtctgaacgg cgagcacaac
     5521 cgttccaacg cagtatcagc actacttgcc gcgcgacacg ttggcgtgcc gttcgatata
     5581 agcctggaag ccttgtcgcg cttcaaaaat gtcaggcgac ggttggaact gcggggaacg
     5641 gtccgcggag tgaacgtcct cgatgacttt gctcatcatc ctactgccat cgcgacgacg
     5701 gtgtccgggc tgcgtcgcag aatcgggtcg ggcgcagaag cgccgcgaat tctcgctgtt
     5761 ctggaaccgc gctcgaacac gatgaagctg ggtgtgatga aggcgcaact gccagcaagc
     5821 ctcgccgacg ccgatctggt gttctgctat tccgccaagc tcggatggga cgcttcagaa
     5881 gctctggcgc cattgggcca caaggcacag gtttccgacg atctggatgg cctgatccgc
     5941 aacatcgtcg ctgctgcccg acatggcgac cagatactgg tgatgagcaa cggtggtttc
     6001 ggcggcattc acgacaagtt actggctgcg ctggccgatt aaaccggaat ctttacagat
     6061 aaagctcata tcgttcgtgc gttggattac acaaaatgat cggccaatag cgcagcaaag
     6121 agcaaagtca gatagatgat cgaccagcgg aaagtgcgtc tggccagtgc atcgctgtag
     6181 ttgcgccaaa gcgcaaaggt gtaggctaaa aagatgacat caagtaccag taccgtggcg
     6241 agataccacg gcccactcat gcccagagtg tagggcaaca ctgtagcccc tgtaagcatg
     6301 acgacataga gaaaactgtg aaaacaggta agctcgattc cgtgcgtgac gggcagcatg
     6361 ggcagaccgg cttttacgta atcctcacgg cgatagcagg cgagcgccca aaagtgcggt
     6421 ggggtccaga tgaaaatgat caggaacaga accaatgctt cagggccgac actgccggtc
     6481 attgctgccc atccgagcaa tgggggcatg gcgcctgacg ctccgccgat cacaatgttc
     6541 atcggcgtag ctggtttcag tacgatcgtg tagatgatcg cgtaacctag aaaggtcgca
     6601 agagtcagcc acatggtcag tggattgacc atggcgtgaa gcagccaaag cccaacactg
     6661 ccaactaatg tggccaaggt cagtgtttct accatcgggg ctttaccgtt tgccgtggcg
     6721 cgccaacggg tacgcgccat cttcgcgtca accgtttttt caaccaggca gttgatcgct
     6781 gctgcagcac ctgcgacgag cgcaatgcca attgatgcag acagaaagac ggttagggga
     6841 gggaagccgg gtgaggcgag aagcatgccg atcatcgctg tgaagacaat caggctattg
     6901 acgcgcggtt tgcacaaacc gaagaaatca gagatccgac gccgagccgt gcggattgaa
     6961 ttcgcctgat ctctaaaggt ggcgggcata cgatatctcc agatagcttt agcgaaccca
     7021 ggaatacttc agcaagcgct caagatcctt cagcacgcca cgcatatcga ttggctcggc
     7081 gtagcgcatc atgacgttac cgagtggatc aacgatgaag accttttgcc cactgtcacc
     7141 caacagactg cgccagacgt tgacctctgt gctggctttt gccattgcaa cgacaatatc
     7201 cgggaagtgc tgctgcaatt tcagtagtgc tggcgagcta tgagtaccag aagaatcaat
     7261 gctcacgaga acccgctgca ggcggtcttg ctccttgttt agtgcgatgt gaacctgctg
     7321 catcagttgc aggctttttt cgcaatcctt gtcgcatgcg ccgttgacgg gcagtacgag
     7381 taaccacttt cctcgaaagt ccgaggccgg caggggtcgt ccatcggcat ggctgagacc
     7441 ggtttccggc agtgggatcg gtggctggag aagatctcca tggttaatgt atttctctgg
     7501 gcgccaatcc agccagaaga gccctgaacc gacaacgaag ggaagaacaa ataccgcagc
     7561 aagtaacagc ag
//
LOCUS       DAORPM010000104         7557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2460_length_7557_cov_12.293788, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000104 DAORPM010000000
VERSION     DAORPM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2460_length_7557_cov_12.293788"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLD53_06505"
     CDS             <1..275
                     /locus_tag="PLD53_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRC60598.1"
                     /translation="ADASKQPEQPNEVSVAVTASGQVLVNKSPLVATDVNAISEALRR
                     AAGDSKDPVIVINADAKATHQSVVDIMQAAQTAGYPHISFATQSPR"
     gene            319..1365
                     /gene="lpxK"
                     /locus_tag="PLD53_06510"
     CDS             319..1365
                     /gene="lpxK"
                     /locus_tag="PLD53_06510"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRC60599.1"
                     /translation="MGSCRIAQRLPRLWYRRQLAVALLPLLPFSWLFRLIVWARFALY
                     RFGALRSYRLPVPVIVIGNLTVGGSGKTPLVIWLVERLSELGWRPGIISRGYGGAIEG
                     VQPVAADSDASVVGDEPLLLARRVNVPVFVGRDRVAAGQALLDNHPECNVIVSDDGLQ
                     HYRLQRAVEIAVFDGRGAGNARLLPAGPLREPLQRLAAVSAVVRSATPLAQKEDATRG
                     VPHFEMRLVGQRFYLLTDAQHSCSADDLQGKRLYAMAGIGDPSRFFRQLEALGLEFEA
                     RPFPDHHIYRAADLAFAHDGVVLMTEKDAVKCAALVAGVAWVLPVEAQVISASADHSL
                     LDIILEKIDGRALA"
     gene            1346..1522
                     /locus_tag="PLD53_06515"
     CDS             1346..1522
                     /locus_tag="PLD53_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRC60600.1"
                     /translation="MDARLLDILVCPICKANLEYHKAEAELICKPCKLAFPVRDDIPV
                     MLEEEARHLDAQET"
     gene            1538..2302
                     /gene="kdsB"
                     /locus_tag="PLD53_06520"
     CDS             1538..2302
                     /gene="kdsB"
                     /locus_tag="PLD53_06520"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRC60601.1"
                     /translation="MKFKVVIPARYASTRLPAKPLLDLGGKPMVARVAERAVLSGAEE
                     VWVATDHADIQEAAARHGLSVMLTDANHPTGTDRLAEVVAQRGWDDETIVVNVQGDEP
                     LIDPELVAQVASQLASSGADIATLAHPITLAADFLNPNVVKVVCKANDDALYFSRAPI
                     PYPRDHFAGDAGNDTLPSGIPAYRHIGLYAYRARFLRAFSRLKASPLEEFEALEQLRA
                     LWHGFRINVLITNHQPLPGVDTREDAVRMQECFRKM"
     gene            2431..3084
                     /gene="adk"
                     /locus_tag="PLD53_06525"
     CDS             2431..3084
                     /gene="adk"
                     /locus_tag="PLD53_06525"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRC60602.1"
                     /translation="MRLILLGAPAAGKGTQAQFICEKFGIPQISTGDMLRAAIKAGTA
                     LGIEARKVMDAGGLVSDDIIIGLVKERLKQADCAAGYLFDGFPRTIPQAEALRSAGTA
                     LDFVLEIDVADSEIIERMSGRRVHLASGRTYHVKHNPPKIEGQDDVTGEALIQRDDDR
                     EETVKKRLQVYHSQTQPLIEYYSKWFATGDVKAPKCRKVAGVGSIEQIKQAAFAALK"
     gene            3261..5324
                     /locus_tag="PLD53_06530"
     CDS             3261..5324
                     /locus_tag="PLD53_06530"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRC60603.1"
                     /translation="MSAGLVFALVCAVVAVLYGAVMTKWILSLPAGNDRMQEIARAIQ
                     VGASAYLNKQYTTIGVVGAVLAILIAIFLGKLMAVGFVIGAVLSGATGFIGMNVSVRA
                     NVRTAEAARSGVAAALDVAFKGGAITGMLVVGLGLLGVAGYYAYLRGGGADFQHAVEP
                     LVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDN
                     VGDCAGMAADLFETYAVTVVATMVLGAMMLKGVANANDNLIIYPLVLGGVSIIASIIG
                     CFFVKANDGGKIMNALYRGLAVAGILALLAFYPVTSWMLGDGIMLADGKLVSSFSIFC
                     AATVGLVLTGLLVWITEYYTGTDFSPVQHVAEASTTGHGTNIIAGLGVSMKATALPVL
                     SVCLAIYVTYALAGLYGISIAATSMLSMTGIIVALDAYGPITDNAGGIAEMSGLPQEV
                     RDVTDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALESKMGGVVMSFDLSDHM
                     VIIGLFLGGLIPYLFSAMAMEAVGRAAGSVVIEVRRQFKELPGIMDGTDKPDYSRAVG
                     MLTTAAIKEMIVPSLLPVLVPVVVGLLLGPKALGGLLMGTIITGLFVAISMTTGGGAW
                     DNAKKYIEDGHFGGKGSEAHKASVTGDTVGDPYKDTAGPAVNPLIKIINIVALMIVPI
                     LPVVA"
     gene            complement(5388..5696)
                     /locus_tag="PLD53_06535"
     CDS             complement(5388..5696)
                     /locus_tag="PLD53_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HRC60604.1"
                     /translation="MRFSSNTPFIIVGITGDGTRFRPSDWAERLCGVLSAFGAEKKMK
                     YSPYVSPGQYKAEKAVFVDGRFYEIEPMAYRFILNFAQDNDLQLIEGVRRNENKIDTI
                     "
     gene            5918..>6115
                     /locus_tag="PLD53_06540"
     CDS             5918..>6115
                     /locus_tag="PLD53_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60605.1"
                     /translation="MQLSPTAKYGFRIRTRSGAVVDNLLIFGKDAPDAERKLRQIYLG
                     CEILEAKRLLVQMPRVGMVNFE"
     assembly_gap    6116..6125
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6250..7020)
                     /locus_tag="PLD53_06545"
     CDS             complement(6250..7020)
                     /locus_tag="PLD53_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRC60606.1"
                     /translation="MRIAVFNQKGGVGKTTTTLNLAAAVARDGGQPLLLDLDPQCHLS
                     HIHGSIPRDSARSLFGFYQGLHALDELAVPWEGLGRIVPSHQQLIKVDSIFGKGPAVL
                     NKLRLALDAMDESIPGNAAQNSLIDCCPYVGVLALNAIFACDALLIPISTDYLSLLAA
                     DQMTRTLAILEPVLKRRVERRYLLTRYDRRRRMSKDVQTQLLERYGEEVCRTVISENV
                     AIAESPSMNRDVFSHNASSVGAKDYTALYGELRQQGLL"
     gene            7238..>7557
                     /locus_tag="PLD53_06550"
     CDS             7238..>7557
                     /locus_tag="PLD53_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC60607.1"
                     /translation="MSHLMNTYARLPVAFSHGDGSWVTDTEGKIYLDALSGIAVSTLG
                     HNHPELVAALAAQCGRLLHTSNLYAMPQQEQLADKLAALAGMDEVFFCNSGCEANEAA
                     IKLAR"
BASE COUNT         1501 a         1976 c         2271 g         1799 t
ORIGIN      
        1 cggccgacgc cagcaaacag cccgagcaac ccaatgaagt cagcgtagcc gtaactgctt
       61 ccgggcaggt actggtcaac aagtctccct tggttgcaac tgacgtgaac gcaatcagcg
      121 aagcgttgcg ccgcgctgcg ggcgattcca aggatccggt gattgtcatc aatgccgatg
      181 ccaaggcaac gcaccagagc gtggtcgata tcatgcaggc ggcacagacg gccggttatc
      241 cgcacatatc cttcgctacc caatcgccgc gctgatggcg catcgctggt tcgccgcctg
      301 cttgttgata atttgctgat gggttcttgc cgtatcgcac agcgactgcc ccgcttgtgg
      361 taccggcggc agctggccgt tgcgctcctc cccttgctac ctttttcgtg gctgtttcgg
      421 ctgattgtct gggcacgatt tgccttgtat cgctttggag cgttgcgctc ctaccgcctg
      481 ccggtgccgg tcatcgtgat aggcaacctg accgtcggtg gtagcggcaa gacgccgctt
      541 gtgatctggc tggttgagcg cttgagcgag ttgggctggc gaccggggat tatcagccgc
      601 ggctatggtg gtgcgattga gggagtgcag ccggttgctg ctgattccga cgcttccgtc
      661 gtaggggatg agccgctgtt gctggctcgg cgcgtcaatg tgccggtttt tgtcggtcgc
      721 gatcgcgtgg ctgcgggtca ggctttgctg gacaatcatc cggagtgcaa tgtcatcgtt
      781 tcagatgacg gcctgcagca ctaccgcttg cagcgcgctg tcgaaatcgc cgtatttgac
      841 ggacgcggtg cggggaatgc ccggttgttg ccggcgggac cgcttcgaga gcctttgcaa
      901 cgtttggcgg cggtatcggc ggtggtgcgg agcgcgaccc cactggcaca gaaggaggac
      961 gccacgcgcg gcgttcctca ttttgagatg cgcttggtgg ggcaacgctt ctacttgctg
     1021 acggacgcgc agcacagctg ttccgctgac gatctgcagg gcaaacggct gtacgcgatg
     1081 gccggtattg gcgatccttc ccgtttcttt agacagcttg aggctttggg ccttgagttt
     1141 gaggcacgtc cattccctga tcatcacata taccgtgctg ccgatcttgc ctttgcgcac
     1201 gatggagtag tgctgatgac cgagaaagat gcggtaaaat gcgccgcgtt ggttgcaggt
     1261 gtggcttggg ttctgccggt agaggcgcaa gtcatttcag catccgctga ccattccttg
     1321 cttgacatta ttctggagaa aatcgatgga cgcgcgcttg cttgatatcc tcgtctgccc
     1381 gatttgcaag gcgaatctcg agtaccacaa ggcggaggcg gagctgatct gcaagccatg
     1441 caaactggct ttcccggtgc gcgatgacat cccggtgatg cttgaagaag aggcgcgtca
     1501 ccttgacgca caggaaacct gaatttcagg tggcggcatg aaattcaagg ttgtcattcc
     1561 tgcccgttac gcatcgaccc gcctgccagc caagcccctg cttgatctcg gtggcaagcc
     1621 catggtcgcc agggtggccg agcgtgcggt cctttcaggt gccgaagaag tctgggtcgc
     1681 gaccgatcac gccgatatac aggaggccgc tgctcggcat ggcctgtcgg tgatgcttac
     1741 cgatgcaaat catccgaccg ggaccgatcg tctggctgag gtcgttgcgc agcgcggctg
     1801 ggacgacgaa accatcgtgg tcaatgttca aggcgacgag ccattgattg atccagagct
     1861 ggttgctcag gtggcgagcc aactggcttc gagcggagcg gatatcgcta ccttggcgca
     1921 tccgatcacg ctcgctgccg atttcctaaa tccgaacgtg gtcaaagtgg tgtgcaaggc
     1981 gaacgacgat gcattgtact tttcccgcgc tccgattcct tacccgcgcg atcactttgc
     2041 gggcgatgcc ggaaatgaca ccttgccttc cgggattcct gcgtatcggc atatcggctt
     2101 gtatgcctat cgcgcccgtt ttttgcgggc gttttcacgt ttgaaggctt caccgcttga
     2161 ggaattcgag gcgctggaac agctacgcgc cctctggcac gggttccgta tcaatgtact
     2221 gattaccaat catcagccat tgccgggagt cgatacgcgg gaagacgcgg ttcgaatgca
     2281 ggaatgcttt cgcaagatgt gaaaacgttt gaccgacagg ccaattgaag gtacttttca
     2341 gtaccatttt tgaagacgtt ggcaagtcac catgttgagc tggtggtata ctaaatcgtt
     2401 tagttcgatt ctctccgagg aagcagggca atgagactaa tcttgctggg tgcgccagcc
     2461 gccggtaaag gcacacaggc acaattcatc tgtgaaaaat tcggcattcc tcagatttca
     2521 accggcgata tgctgcgtgc cgcgataaag gccggtactg ccctcggaat tgaggcgcgc
     2581 aaggtcatgg atgcgggtgg tttggtgtcc gacgacatca tcatcgggct ggtcaaggag
     2641 aggctcaagc aggcggactg cgcagcgggc tatctgtttg acggattccc gcgaacgatt
     2701 ccgcaggcgg aggccttgcg gtctgccggt acggctctgg atttcgtgct ggaaatcgac
     2761 gtggccgatt cggaaatcat cgaacggatg agcggtcgcc gggttcatct ggcatcggga
     2821 agaacatacc atgtcaaaca caatccgcca aagatcgagg ggcaggacga tgtcacgggt
     2881 gaagcgctga ttcaacgtga cgatgatcgc gaagaaaccg tcaagaagcg tctgcaggtt
     2941 tatcattcgc aaacacagcc attgatcgag tattacagca agtggtttgc cacgggcgat
     3001 gtcaaggcgc ccaaatgccg caaagttgcc ggcgttggca gtatcgagca gatcaagcag
     3061 gctgcgtttg cagcattgaa atagtttcgt cgtcgaaaat ccgcagatcg ggatattcga
     3121 agggttcaaa atttggttgc ggtatagcaa gcaatacctc ccacaagagg aggcgtgaac
     3181 aggtttaaag cagatcgttg agcaatccgg tgcccaagga gtttcccgac gggccagagt
     3241 aaacttgata gggggtattc atgtcggcag gtctcgtatt tgcgctggta tgcgcggtgg
     3301 tcgcggtgct atatggtgcg gtgatgacca agtggattct ttcgttgccg gcaggcaatg
     3361 atcgtatgca ggaaatcgcc agggcgattc aggttggcgc atcggcctac ctgaacaagc
     3421 aatacacgac gataggcgtc gtcggcgctg ttcttgctat cctgatcgca atatttctgg
     3481 gcaagctgat ggctgttggt tttgtcatcg gtgccgtgct ctcgggtgcc accggcttca
     3541 ttggcatgaa cgtgtcggtg cgagccaatg tgcgaaccgc cgaggcggcg cgtagcggtg
     3601 ttgctgccgc acttgatgtg gccttcaagg gtggggcaat cacgggtatg ctggttgtcg
     3661 gcctcgggct gctcggtgtg gcgggctact atgcgtactt gcgcgggggc ggtgccgatt
     3721 ttcagcatgc agtcgagccg ctggtggggc tggctttcgg cggatcgctg atttccatct
     3781 ttgcccgact gggcggaggc atattcacca agggcgctga cgtcggcgcc gatctggttg
     3841 gcaaggtcga ggctggcatc cctgaagacg atccgcgtaa cccggcggtg attgccgata
     3901 acgttggcga caacgttggc gattgtgccg gtatggctgc cgaccttttc gaaacctacg
     3961 ccgttacggt ggtggctaca atggtgcttg gcgcgatgat gctcaagggt gtggccaatg
     4021 ccaacgacaa cctgattatt tatcctttgg ttctgggtgg tgtttcgatc attgcttcga
     4081 tcatcggttg tttcttcgtc aaggccaatg atggcggcaa gatcatgaat gctctctatc
     4141 gtggtctcgc ggtggctggt attttggcgt tgcttgcttt ctacccggta acaagctgga
     4201 tgctgggcga cgggatcatg cttgctgacg gtaaattggt cagctcgttc agcatattct
     4261 gtgcggcgac ggttggcctg gtgctgacgg gtttgctggt gtggattacc gagtactaca
     4321 cgggaaccga tttctcgcca gtacaacatg tggcagaagc ttcgacgacc ggacacggca
     4381 ccaacatcat cgctggtttg ggcgtctcga tgaaggcaac cgcgctgccg gtactctccg
     4441 tctgtctggc gatctacgtt acctatgcat tggcagggct gtatggcatt tccatcgccg
     4501 cgacctcaat gctgtcgatg accggaatca tcgtcgcgct cgatgcctac gggccgatta
     4561 cggataatgc aggcggtatc gccgaaatgt ccgggttgcc gcaggaagtg cgtgacgtga
     4621 ccgatccgct tgatgccgtg ggcaatacca ccaaggcagt aaccaagggt tacgccattg
     4681 gctcggcggg cttggctgcg ctggtgctgt ttgccgacta cacgcacgcg cttgagtcca
     4741 agatgggtgg tgttgtcatg tcttttgacc tttccgatca catggtgatc atcggcctgt
     4801 ttctcggcgg gctgattccg tatctgttca gcgccatggc gatggaggct gtcgggcgcg
     4861 ccgcgggttc ggtggtcatc gaagtgcgcc ggcaattcaa ggagcttccg ggaatcatgg
     4921 acggaacgga caagccggat tactcccggg cggtcggtat gctgaccacg gcggcgatca
     4981 aggaaatgat cgttccgtcg ctcctgcctg tgctggtgcc ggtggttgtt ggtctgctgc
     5041 tggggcctaa ggcgctcggc ggattgttga tgggaacaat cattaccggt ctgtttgtcg
     5101 ctatttccat gacgaccggc ggaggagcct gggataacgc caagaagtac attgaagacg
     5161 gtcacttcgg tggcaaaggc tctgaagccc acaaagcctc ggtgaccggc gataccgttg
     5221 gcgaccccta caaggacacg gctggaccgg ccgtgaatcc gctgatcaaa atcatcaata
     5281 tcgttgcgct gatgatcgtg ccgattcttc ctgtagtggc ctgattccag gtagtcaaag
     5341 caatgaaaag gggcagctta cgctgcccct tttcttttca tcgtcgatca gatcgtgtcg
     5401 atcttgttct cgttgcgcct gacaccttcg ataagttgca ggtcattgtc ctgcgcaaag
     5461 ttgaggatga agcggtatgc catgggctcg atctcgtaga accgaccatc cacgaaaacc
     5521 gctttttcgg ccttgtactg gccggggctg acataaggcg agtatttcat tttcttttcg
     5581 gcaccaaatg ccgatagaac gccgcacagc ctctccgccc agtcactggg gcggaaacga
     5641 gtgccatcac cggttattcc gacaatgatg aatggggtat tggaggaaaa tcgcatcttt
     5701 tggtcgcaaa aaacagtttc ttagtgttga aatcttgctt gtcttgtgaa cgcgcggatt
     5761 ctatcagaaa gtcgcactat cgccgatgcc cggcttcgga tcggcccgaa aacagccatt
     5821 tcgacctatt ggtcatacga aaataatgga cacttgcctt gtattcgtat aatttactgc
     5881 atatagtcgt cacatctctt tgatatagag gtactttatg cagctatcgc ctacagctaa
     5941 atatggtttc cgtatccgca cccgcagcgg agcggttgtc gacaatctgt tgatatttgg
     6001 caaggacgca ccggatgccg agcgcaagtt gcgccagatc tatcttggct gcgagatcct
     6061 cgaagcgaag cgattgctcg ttcagatgcc gcgtgttggc atggtgaatt tcgaannnnn
     6121 nnnnngccag gcgttctgtc attcaggcgc cgcgtaccgg cccggtgacc tatgaagatg
     6181 ttgtcgatct catatcggga ccggcttccg tcgagacaag caaatagcgg ttcctgcccg
     6241 aaactctcct caaaggagac cttgttgtcg aagctccccg tagagggccg tgtagtcttt
     6301 ggcgccaacg ctggaagcat tgtggctgaa aacatcccgg ttcattgagg ggctttctgc
     6361 aattgccaca ttctccgaaa taacggttct gcatacttct tcaccataac gctcgagcaa
     6421 ttgcgtctgc acgtccttgc tcattctgcg ccggcgatca taccttgtca gaagatagcg
     6481 gcgctcgacg cgccgcttga gtaccggttc gagaatggcc agcgttcgag tcatttggtc
     6541 tgctgccagc aaggacaggt aatcggttga aatcgggatg agcaatgcat cgcacgcaaa
     6601 tatggcattg agcgccaaaa cgccgacata agggcaacaa tcgataagcg agttctgcgc
     6661 tgcgtttccc ggtatcgact catccatggc atcaagggca aggcgcaatt tgttcagaac
     6721 tgccgggcct ttgccaaaaa tcgagtcgac cttgatcaat tgttgatggg acggaacgat
     6781 cctgccaagg ccttcccacg gaacggccag ttcatcaagc gcatgcaagc cttgataaaa
     6841 cccgaagagg ctgcgtgctg aatcccgtgg tatggatccg tggatgtgcg aaaggtggca
     6901 ctgtgggtca aggtcaagca acaatggctg cccaccgtca cgggcaacgg cggccgcgag
     6961 gtttagcgtc gttgttgtct tgccgacgcc acctttctgg ttgaaaacag cgatgcgcat
     7021 aacgcattgt agcgcgctat ctgcatcccg atcgcttttc gagcttatat acaagccact
     7081 ggttttccct tagaattcca ccccttgcaa tatcggcagg cagttctcgc gaacgagtac
     7141 gagcaggtac cggttcagtg ctgatgcgtg atgatgcgtg actctccggc ggcgccagcc
     7201 gccgtttcag tttcagagcc tcatcaaggg gttgtgtatg tctcacctaa tgaacactta
     7261 tgcccggttg ccggtcgcct tcagtcatgg cgacggtagc tgggtaaccg acaccgaggg
     7321 caaaatctat ctcgatgcgt tgtcgggaat cgccgtttcc acacttgggc ataatcatcc
     7381 tgagttggtg gccgcgcttg ctgcgcagtg tggacgcctg ttgcatacct ccaatctcta
     7441 tgcaatgccg cagcaggagc agttggccga caagctcgcc gccctggcag gaatggatga
     7501 ggtgtttttc tgcaattcgg gttgtgaagc caacgaagcg gcgatcaagc ttgcgcg
//
LOCUS       DAORPM010000105         7530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2474_length_7530_cov_12.158796, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000105 DAORPM010000000
VERSION     DAORPM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2474_length_7530_cov_12.158796"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1011)
                     /locus_tag="PLD53_06555"
     CDS             complement(115..1011)
                     /locus_tag="PLD53_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HRC60608.1"
                     /translation="MMPLPSLKQLQYLVALSEQLNFTRAAEACFVSQPTLSAGLKELE
                     NILGTQLVERDRQVVLMTRIGLEIARRARVILAATHDLADLAAGALEPMTGRLRLGVI
                     PTIAPFVLPLSLPLLRERYPELQLALREDVTANLLLRLEDGQLDFALIALPYDTANLL
                     VETLFDDALWLVGRKDDPEVQPKTIRVSPSLTDHLLLLEEGHCLRDHALYACGESSHQ
                     SSTDMEATSLLTLVQMVESGLGIALVPEMAVRSGLTKSPGLVARPMSEPSPKRTIALV
                     ARRSTSRMEELRILAGVLRTAF"
     gene            1169..2620
                     /locus_tag="PLD53_06560"
     CDS             1169..2620
                     /locus_tag="PLD53_06560"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRC60609.1"
                     /translation="MSNKLTTAAGCPVVDNQNSMTAGQRGPVLMQDVWLLEKLAHFDR
                     EVIPERRMHAKGSGAYGTFTVTNDITKFTRAKVFSRVGKKTDLFARFTTVAGERGAAD
                     AERDIRGFAVKFYTEEGNWDMVGNNTPVFFLRDPLKFPDLNHAVKRDPRTNMRSAKNN
                     WDFWTSLPEALHQVTIVMSDRGLPATYRHMHGFASHTFSLINARNERFWVKFHFKSQQ
                     GIKNLTDAEAEAIIGQDRESHQRDLFESIENGDYPKWTMYVQIMPEADAARVPYNPFD
                     LTKVWPHKDYPLIEVGVMELDRNPENFFAEVEQSAFNPAAVVPGIGFSPDKMLQARLF
                     SYGDAQRYRLGVNHHQIPVNAPRCPVHSYHRDGSMRVDGNHGGTLGYEPNDQGEWAEQ
                     PDFSEPPLSIDGAADHWNHREDTDYFSQPGNLFRLMSPAQQQVLFENTARSVGGAPKE
                     IQLRHIRNCLKADPAYGQGVANALGVPVNELGA"
     gene            complement(2617..3702)
                     /gene="hisC"
                     /locus_tag="PLD53_06565"
     CDS             complement(2617..3702)
                     /gene="hisC"
                     /locus_tag="PLD53_06565"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRC60610.1"
                     /translation="MNPFWSTLVHNLSPYVPGEQPKLPDLVKLNTNENPYPPSARAVD
                     AMREELGSDGASLRLYPDPNADRLRGAIVNHFADQALTPAQVFVGNGSDEVLAHAFMA
                     LLKHDAPILLPDISYSFYPVYCGLYGVDHITVPLNERFEICVDDFRQDNGGIVIANPN
                     APTGHLLRLAEIERLVAAHPTSVVVVDEAYIDFSEQVVPTAIALINRYPNLLVIRTLS
                     KSHSLAGLRVGYAVGNAALIDALERVKNSFNSYPLDRLAIVGATAALGDKPHLEQTRQ
                     AVMQSRASLTDALERLGFDVLPSAANFIFTRHPQHDAAQLATELRQRKVIVRHFKLPR
                     IDQHLRITVGTDTQCQLLVDALREILG"
     gene            3845..4432
                     /gene="hisB"
                     /locus_tag="PLD53_06570"
     CDS             3845..4432
                     /gene="hisB"
                     /locus_tag="PLD53_06570"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRC60611.1"
                     /translation="MRQAEISRNTLETRITVRLNLDGSGQSRLATGVPFLDHMLDQIA
                     RHGMLDLEVEAQGDLHIDAHHTVEDIGITIGQALVKAWGDKNGLRRYGHAYVPLDEAL
                     SRVVIDLSGRPGLVFNVEFKRAMVGAFDVDLTHEFFQGLVNHALMTIHVDNLRGENAH
                     HQAETVFKAFGRALRMAVEADPQAAGSIPSTKGSL"
     gene            4444..5082
                     /gene="hisH"
                     /locus_tag="PLD53_06575"
     CDS             4444..5082
                     /gene="hisH"
                     /locus_tag="PLD53_06575"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRC60612.1"
                     /translation="MGSIAVIDYGMGNLRSVWQALVHVADGRDVVVTADPAVVAAAER
                     VVFPGQGAMPDCMRELDARGLRQVVLETARTKPFLGICIGLQMLFEHSDEGDVSGLGV
                     FAGNVRRFAAERMVNASGEKLKVPHMGWNEVRQIRQHPLWTGIPDGARFYFVHSYCVT
                     PDDADCIAGMTDYGTPFTSVVARDNIFAIQCHPEKSAKAGLALLSNFLQWKP"
     gene            5143..5886
                     /gene="hisA"
                     /locus_tag="PLD53_06580"
     CDS             5143..5886
                     /gene="hisA"
                     /locus_tag="PLD53_06580"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRC60613.1"
                     /translation="MLIIPAIDLKDGECVRLKQGLMDDATVFNTSPGEQAALWLEQGA
                     RRLHVVDLNGAFAGKPKNESAIKEIVKALGDEIPIQLGGGIRDLDTIERCLDDGVSYV
                     IIGTAAVKNPGFLHNACSAFPGQIIVGLDARDGKVAVDGWSKLTGHDVIDLAKKCEDY
                     GVEGIIYTDIGRDGMLSGINIEATVKLAQALMIPVIASGGLSSMEDIRQLCAVEHEGI
                     FATIAGRAIYDGSLDFAEAQEEADRATQN"
     gene            5939..6700
                     /gene="hisF"
                     /locus_tag="PLD53_06585"
     CDS             5939..6700
                     /gene="hisF"
                     /locus_tag="PLD53_06585"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRC60614.1"
                     /translation="MGLAKRIIPCLDVTAGRVVKGTNFIDLRDAGDPVEIARRYDDQG
                     ADEVTFLDITASSDQRDIILHIVEACAAQVFIPLTVGGGVRTVADVRRLLNAGADKVS
                     MNTAAVENPDLVAEASDKVGNQCIVVAIDAKQVAPGQWEVFTHGGRKRTGLDAVEWAC
                     RVQQLGAGEILLTSMDRDGTKNGFDLPLTRAVSDAIDIPLIASGGVGNLQHLADGVTQ
                     GHADAVLAASIFHFGEYSVLQAKEFMRERGIEVRL"
     gene            6697..7110
                     /gene="hisI"
                     /locus_tag="PLD53_06590"
     CDS             6697..7110
                     /gene="hisI"
                     /locus_tag="PLD53_06590"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRC60615.1"
                     /translation="MSDLDTSLNWLDALKWDESGLITTIAQDATSGRVVMCAWMNRDA
                     LRETVASGNAVYWSRSRGRLWRKGEESGHFQKVLSIRADCDGDVLLLSIEQVGGIACH
                     TGRESCFFLELQGERWVPADPVLKDPKAIYKSNSQ"
     gene            7350..>7530
                     /locus_tag="PLD53_06595"
     CDS             7350..>7530
                     /locus_tag="PLD53_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60616.1"
                     /translation="MSSFAPRCLSIDLEVGLRDTRIHRFAAVRGDQPEASLHFRQGNL
                     PAALEQLDALAEGASF"
BASE COUNT         1561 a         2107 c         2297 g         1565 t
ORIGIN      
        1 cgaacggtct acccctattg cgtcgtcggc gatcggctgc cgctcaccag cgccgcgacg
       61 tttgaaacct cctcagctct tggcaatcac gtgcgcaagc catgcgctgg ttcttcagaa
      121 tgctgttcgt aaaacaccag ccagtattct gagttcttcc atgcgcgagg tggagcgtct
      181 cgcgacgagg gcgatggtgc gcttggggct gggctcgctc atcgggcgtg cgaccagacc
      241 ggggctcttc gtcaatccgc tccttaccgc catttccggc actagcgcga taccaagccc
      301 ggattcgacc atttgcacca gggtcagcag gctggtcgct tccatgtccg ttgacgattg
      361 atggcttgat tctccgcagg catacagtgc gtgatcgcgc aggcaatgcc cctcttcgag
      421 gagcaacagg tgatcggtca gtgatggcga cacacggatc gttttcggct gaacttccgg
      481 atcgtctttc cgcccgacga gccacagggc gtcgtcgaag agcgtctcta cgagcaggtt
      541 tgccgtgtca tacggcaggg cgatcagggc aaaatcgagt tggccgtctt ccaggcgcag
      601 cagcagattg gccgtcacat cctcgcgcag tgccagttgc aactcaggat agcgttcgcg
      661 caacaagggc agggaaagcg gtaacacgaa gggggcgatg gtcgggatga cgccgaggcg
      721 cagtcgccct gtcatcggtt ccaacgcccc ggccgccaag tcggccagat cgtgcgttgc
      781 ggcgagtatg acgcgtgccc ggcgagcgat ttccagcccg atgcgggtca tcagaacaac
      841 ctgcctgtcg cgctcgacca gttgcgttcc gagaatattt tccagctcct tgagtccggc
      901 actcagggtt ggctggctta caaaacaggc ttctgccgcg cgtgtgaaat tgagctgctc
      961 ggagagggcg accaggtatt ggagttgctt gagcgatggc aatggcatca tggtgtgctc
     1021 ggcgcgttat tgagctattc gataaacaat caaaatatta ttcatttagg aattatagcc
     1081 acattgtcta cagtgagttt gaacacgtcg tctcgacgac ctgcccattt gagctatcaa
     1141 cgttttcaac ctcgaaagga gtctcgatat gagcaacaaa cttaccactg ccgcaggttg
     1201 cccggtagtt gacaaccaga acagcatgac cgccggtcag cgcgggccgg tcctgatgca
     1261 ggatgtctgg ctgctcgaaa aactggcgca cttcgaccgc gaggtcattc ccgagcggcg
     1321 catgcatgcc aagggttctg gtgcctatgg cacgttcacc gtgacgaacg atattacgaa
     1381 attcacccgg gcgaaggtat tctctcgggt cggcaagaaa accgatcttt tcgcccgttt
     1441 cactacggtg gccggagaac gaggcgccgc cgacgccgag cgcgatatcc gcggttttgc
     1501 cgtcaagttc tacaccgaag aaggcaactg ggacatggtt ggcaacaaca cgccggtatt
     1561 ctttctgcgc gacccgctca agtttccgga cctcaaccac gcggtcaaac gcgaccctcg
     1621 caccaacatg cgcagtgcga agaacaactg ggatttctgg acatcgctac ccgaggcatt
     1681 gcatcaggtg accatcgtca tgagcgaccg tggcctgccg gcgacctatc gtcacatgca
     1741 cggctttgcc tcgcacacct tcagcctgat caatgcgcga aatgagcgtt tctgggtcaa
     1801 gttccacttc aagtcgcagc agggcatcaa gaacctgacc gacgccgagg ccgaagcgat
     1861 tatcggccag gatcgcgaga gtcatcagcg cgatctcttc gagagcatcg aaaatggcga
     1921 ctatccaaag tggacgatgt acgtacagat catgccggag gcggatgccg caagggtacc
     1981 gtacaacccc ttcgacctga ccaaggtgtg gccgcacaag gattacccgt tgatcgaagt
     2041 gggtgtgatg gagcttgacc gcaacccgga aaacttcttc gccgaagtcg agcaatcggc
     2101 tttcaatccg gccgctgtgg tgccggggat cggcttctcg ccggacaaga tgctgcaggc
     2161 gcgtctcttc tcctacggcg atgcgcagcg ttaccggttg ggcgtcaatc accatcagat
     2221 tcccgtgaac gccccgcgtt gccctgtcca tagctatcac cgcgacggca gtatgcgggt
     2281 ggatggcaac cacggcggta cgcttggtta cgaacccaac gaccagggcg aatgggccga
     2341 gcagccggac ttctccgagc cgccgctgag catcgacggc gcggccgatc actggaacca
     2401 ccgcgaagac accgactact tctcgcaacc aggcaatctc ttccgcctga tgagcccggc
     2461 acagcaacag gtgctgttcg agaatacggc acgctcggtg ggaggggcgc caaaggaaat
     2521 tcagttgcgc cacatccgca attgcctgaa agccgacccg gcctatggac aaggcgttgc
     2581 caatgccttg ggcgttccgg tgaacgagct tggcgcttag ccgagaatct ccctaagcgc
     2641 atcaaccagc aattggcact gcgtatcggt tccaaccgtg atgcgcaggt gctgatcgat
     2701 gcgcggcaat ttaaagtgac ggacgatgac cttgcgctgg cgaagttcgg tggccagttg
     2761 tgcggcatcg tgctgcggat gccgcgtgaa gatgaagttg gccgccgaag gcagaacgtc
     2821 gaagcccagt ctttcaaggg cgtcggtcag tgaggcacga ctttgcatga cggcttgtcg
     2881 cgtttgctca aggtgtggtt tgtcgcccag tgcggcggtg gcgccgacaa tggccaggcg
     2941 gtcgagcggg taggaattga agctgttctt gacgcgttcc agggcgtcga tcaaggcagc
     3001 atttccaacg gcataaccga cgcgaagtcc ggccagcgaa tgcgatttgg aaagcgtccg
     3061 gatcaccagc agattcgggt aacgattgat cagcgcgatg gccgtgggaa cgacctgttc
     3121 gctgaagtcg atgtaggctt cgtcgacgac cacgactgac gtcggatgag cagccaccag
     3181 acgttctatc tcggccagcc gcagcaggtg gccggtcggt gcgttggggt tggcgatgac
     3241 gatgccaccg ttgtcttgcc ggaagtcgtc gacgcaaatt tcaaagcgtt cgttgagcgg
     3301 aacggtgatg tgatccacgc cgtataaccc gcaatacacc ggataaaagc tgtacgaaat
     3361 gtcgggcaga aggatcggcg catcgtgttt caggagcgcc atgaacgcgt gcgccaacac
     3421 ctcgtcggag ccgttgccga caaaaacctg agccggcgtg agcgcttgat ctgcgaagtg
     3481 attgacgatt gctcccctga gccggtcggc gttggggtcg gggtagagtc gcaggctggc
     3541 tccgtcgctt cccaattcct cccgcatcgc atcgaccgcg cgggccgacg gcggataggg
     3601 gttttcattg gtgttcagtt tgaccagatc gggcagtttg ggctgctcgc ccggaacgta
     3661 gggcgacagg ttgtgaacga gggtgctcca gaacggattc atcaaaggct cgcaaggtcg
     3721 aatgcagcgg aagcggtaaa tggtatcatg cgcaagcact ggctttatct ggccggagcg
     3781 agcctcaacg gttcaaaccg atggtcggat taatcgattt ttgccacttt gcgacggaaa
     3841 ttacatgcgg caagccgaaa tcagcagaaa caccctggaa acgcggatca cggttcgatt
     3901 gaaccttgat ggcagcggac aaagccgtct ggcgaccggt gtccccttcc tcgatcacat
     3961 gctcgaccag attgcacgtc acgggatgct ggatcttgag gtcgaggcgc agggcgatct
     4021 gcacattgac gcacatcaca ccgttgaaga catcggcatc acgatcggtc aggccctcgt
     4081 caaggcgtgg ggtgacaaga acggcttgcg acgttatggg cacgcctatg tgcctcttga
     4141 cgaagcgttg tcgcgcgtcg tgatcgacct ttccgggcgt ccggggctgg tattcaatgt
     4201 cgaattcaaa cgtgcgatgg tcggcgcttt tgatgtggat ctgacccacg agttcttcca
     4261 ggggctggtc aatcacgccc tgatgaccat ccatgtcgac aacttgcgcg gcgagaatgc
     4321 gcatcaccag gccgagacgg tcttcaaagc cttcggccgt gccttgcgca tggcggtgga
     4381 agccgatccg caggcggcgg gcagcatccc ttcaaccaag ggaagccttt gatggcatca
     4441 gccgtgggca gcattgcagt gatcgactac ggcatgggaa atctgcgctc ggtgtggcag
     4501 gcactggtcc atgtggccga tggccgtgac gtggtcgtta ccgccgaccc ggcggtagtc
     4561 gctgcggccg agcgggtggt tttccctggt cagggcgcca tgcctgattg catgcgcgag
     4621 ctcgatgcgc gcggtttgcg gcaggtcgtg ctggagacgg caaggaccaa gccctttctt
     4681 ggaatctgca ttggcctgca aatgctcttt gaacacagcg acgagggcga tgtgtccggt
     4741 ctgggtgtat ttgccgggaa tgttcgccgt ttcgcagcgg aacggatggt taacgcatca
     4801 ggcgaaaagc tgaaggtgcc gcacatggga tggaatgaag tgcggcaaat cagacaacac
     4861 ccgctttgga ctggaattcc cgacggcgcg cgcttctatt tcgtgcacag ctattgcgtg
     4921 actcctgacg atgccgattg cattgccggc atgacggact acggtacccc ctttaccagt
     4981 gtggtggcac gagataatat cttcgccatc caatgccatc cggagaagag tgccaaagcg
     5041 gggctcgccc tgttatccaa tttccttcaa tggaagccct gaacgggtac gccggcacca
     5101 ttctcgttga ctctttaatt tcactacctt tcacttttca aaatgctgat cattcctgcg
     5161 attgacctga aggacgggga gtgtgtccgt ctcaaacaag ggctcatgga cgatgccacc
     5221 gttttcaaca cgtctcctgg cgaacaagcg gcactctggc ttgaacaggg tgcgcgccgc
     5281 ctgcatgtcg tcgatctgaa cggcgctttt gcgggcaaac caaaaaatga aagcgcgatc
     5341 aaggaaatcg tcaaggcgct cggcgatgaa atcccgattc aactcggcgg tggaatccgc
     5401 gatctcgata ccatcgaacg ttgcctggat gatggcgtca gctacgtcat tatcgggaca
     5461 gccgcggtca agaatccggg ttttctgcat aatgcctgca gcgcttttcc ggggcaaatc
     5521 atcgtcgggc tcgacgccag ggatggcaag gtggcggtcg atggttggtc gaagttgacc
     5581 gggcacgatg tcatcgatct ggccaagaaa tgcgaagact atggcgtcga aggcatcatt
     5641 tacaccgata tcggtcgtga cggaatgctc tccggaatca acatcgaggc aacggtcaaa
     5701 ctggcgcaag cgctgatgat tccggtgatc gccagcggcg gcttgtcgag catggaagac
     5761 atcaggcagc tctgcgcggt cgagcacgaa ggcattttcg cgaccatcgc cggtcgggcc
     5821 atctacgatg gctcgcttga ttttgccgag gcacaggaag aggccgaccg cgcgacgcag
     5881 aactagcgcg cttcacaacc aacgtttcac ctttcctacg aaagcagggg aatccgcttt
     5941 gggtctcgct aaacgaatca ttccctgtct tgacgttacc gcggggcggg tggtcaaagg
     6001 aaccaatttc atcgatctgc gcgatgccgg cgatcccgtc gagatcgccc gtcgctatga
     6061 cgatcaaggc gccgatgaag tcacttttct cgacatcacg gcatcgtccg accagcgcga
     6121 catcattctg cacattgtcg aggcttgcgc agcgcaggtg ttcattccgc tgactgttgg
     6181 cggtggcgtg cgcaccgtgg ctgatgtgcg tcgtctgctc aatgcaggcg ccgacaaggt
     6241 cagcatgaac accgcagcag tcgaaaatcc cgacctcgtt gccgaggcat cagacaaggt
     6301 gggcaaccag tgcattgtcg tcgccatcga cgccaagcaa gtggcgccgg gccaatggga
     6361 agtgtttacc catggcgggc ggaaaagaac cggtctggac gccgtcgagt gggcatgccg
     6421 tgtgcaacag ctcggtgccg gcgaaatcct gctgaccagc atggatcgcg acgggaccaa
     6481 gaatggtttc gacttgccgc tgacccgcgc cgtttccgat gccatcgata tcccgttgat
     6541 cgccagcggg ggtgtcggca atcttcagca tctcgccgat ggagtgacac aaggccatgc
     6601 cgatgcggtg ctggcggcca gcattttcca cttcggcgaa tactccgttc tgcaggccaa
     6661 agagttcatg cgcgagcgcg gtatcgaggt gcgcctgtga gcgatctgga caccagtctg
     6721 aactggctcg acgcgctcaa gtgggacgag agcgggttga ttacgaccat cgcgcaggac
     6781 gcaacgagtg gccgggtggt gatgtgcgcc tggatgaacc gggatgcgtt gcgggaaacg
     6841 gttgccagcg gcaacgcagt gtactggtca aggtcgcgcg ggcggctctg gcgcaagggc
     6901 gaggaatccg ggcattttca gaaggtattg tccatccgcg ccgactgcga cggcgatgta
     6961 ttgttgcttt ctatcgagca ggtcggcggg atcgcctgcc acaccggccg ggaaagctgc
     7021 ttctttctgg agttgcaagg cgaacgatgg gttccggccg atccggtatt aaaagacccc
     7081 aaggctattt acaagagcaa ctcacaatag cccgccttgt cttgtaaggt ccggatttta
     7141 ttaggggtct ctgatttatg gaggttcctt ttcatcaggg cgatcgtaca cgcagatgtc
     7201 ggagaccctt gaaaagggtt gtttacaggc catgggttat gcggggcggt ttgcagactc
     7261 ctgccgaagt agcctgcggt acacattaag aatcgtatca gccgcacatg tatgatgtta
     7321 ggttagtgcc cgatacctag atatctcaca tgagtagttt tgctccccgt tgtctttcca
     7381 tcgatctcga agtcggattg cgcgatacgc ggattcatcg ctttgccgcc gttcgtggag
     7441 atcagccgga ggcctcgctg catttcaggc aaggcaatct gcctgccgcg ctggagcaac
     7501 tcgacgcgct cgccgagggc gccagtttcc
//
LOCUS       DAORPM010000106         7506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2489_length_7506_cov_9.899342, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000106 DAORPM010000000
VERSION     DAORPM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2489_length_7506_cov_9.899342"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..446)
                     /locus_tag="PLD53_06600"
     CDS             complement(132..446)
                     /locus_tag="PLD53_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60617.1"
                     /translation="MKKRNLFAEIAEGFDALASERAGKQTLRTHKVEVQPVPEVTADE
                     LVTLRERLHLSRPVFARYLRTNPRTLENWEQGRAKPNAQAALLIRLVEKFPDTVDRLA
                     VV"
     gene            complement(443..781)
                     /locus_tag="PLD53_06605"
     CDS             complement(443..781)
                     /locus_tag="PLD53_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="HRC60618.1"
                     /translation="MKALFVELPAFAKYRADYLDDEGFRILQQAMLKYPEVGNVIEGT
                     GGLRKLRHGDPRRGKGKSGGLRLIYYWWDGGKQFWLFTLYDKDEMEDLSADEKKTLKG
                     MLKAELEARR"
     gene            complement(1005..1748)
                     /gene="phbB"
                     /locus_tag="PLD53_06610"
     CDS             complement(1005..1748)
                     /gene="phbB"
                     /locus_tag="PLD53_06610"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRC60619.1"
                     /translation="MTQRVAFVTGAMGGLGTSICQELAKAGHKVVAAYHPEFDKPDEW
                     TKEMAAAGFKDFICVSGDISSFDSCAAMVAEAEAKAGPIDILVNNAGITRDRMFAKME
                     LSQWDAVIATNLTSLFNMTKQVSAKMAERGWGRIINISSLNGVKGQAGQTNYSAAKAG
                     VIGFSKALAAELAAKGVTVNVIAPGYVATKMVMAIKPEVLQGIIDGVPMKRLAKPEEI
                     GGACAYLASDLAGFMTGATMNINGGLYYQ"
     gene            complement(1918..3570)
                     /gene="phaC"
                     /locus_tag="PLD53_06615"
     CDS             complement(1918..3570)
                     /gene="phaC"
                     /locus_tag="PLD53_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRC60620.1"
                     /translation="MDFLDSQTSVAGVTGSSQELPDPEHFAQLQKRFLDQHQQLWNGM
                     LLRKEDEPLVSVIEADPGDRRFASPEWRSSPIYDYVRQAYLLNSQFIRELVELFPASD
                     GKAKDRARFLARQYIDALSPANFAATNPEFIRLALDTQGKCITDGINNLIGDFGKGRV
                     SISDESAFEVGGNLAMTPGSVIFENELIQLIQYAPTTAKTAQRPLLIVPPCINKFYVL
                     DLQPENSFVRYAVEQGNTVFLVSWRNPKDEQGHLNWHDYLENGPIAAINIVREICNFD
                     QVNVLGWCVGGTILTSALAVLAARGEDKVASLTLLTTLLDYSEAGELGLFIDEQSITE
                     REVEMGKGGLMKGRELSSAFSMLRANDLVWNYVASNYLKGRTPQAFDLLYWNSDSTNL
                     PGPFACWYLRNLYLENALRVPEKLEMCGVKVDLGRVQAPNYVLAAREDHIVPWKAAYL
                     GTQLLGGESRFVLGASGHIAGVINPASKNKRSFWLNTAQQGTTADNWLLDAQEHKGSW
                     WSDWSDWLKQFSEGERGARKKPGNAKYKPIEPAPGRYVKECA"
     gene            4111..5430
                     /locus_tag="PLD53_06620"
     CDS             4111..5430
                     /locus_tag="PLD53_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRC60621.1"
                     /translation="MKFTELGLVPEILRSIADQGYETPTPIQAQAIPVVLSGRDLMAC
                     AQTGTGKTAGFTLPILNRLAASRVSGPRKIRVLILTPTRELAAQVEESVRTYGAHLPF
                     KSLVIFGGVGMNPQISALRNGVDILVATPGRLLDHAQQRNVDLSGVEVLVLDEADRML
                     DMGFIHDIKRVIALVPKKRQNLLFSATFSDEIRTLAGAFLIQPEYVESERRNTAPELV
                     KQFVYKVDRERKRDLLVELIRDKGWYQVLVFTRTKWGANGLADKLLKAGIEADAIHGN
                     KSQNARTRALANFKSGKLHVLVATDIAARGIDIDELPHVVNYELPNVAEDYVHRIGRT
                     GRAGTEGSAISLVCVDEHKLLHDIERLLKKQIEVLAQPGYEPDPSIRAEPILMRSAGA
                     RGGQRSPGGRPGGGGGPRGNSPKAPGGHRPAAGKPGGGSHRSGSRHR"
     gene            5586..6287
                     /locus_tag="PLD53_06625"
     CDS             5586..6287
                     /locus_tag="PLD53_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRC60622.1"
                     /translation="MISTPLLSIIIPVLNEAAGIVSQLEALQALRNAGAILILVDGGS
                     SDNTTELAKPLVDQLIDSSRGRAVQMNTGAQASKSEVLLFLHADTRLPPAADEHIREA
                     IASGALWGRFDIRIDAAHPLLRIVERMMNWRSRLTGIATGDQAIFVRRDLFEETGGYP
                     ELPLMEDIVFTTRLKQIAPPACLRPRVITSGRRWEKHGAARTIFLMWRLRTEFYFGAD
                     PAKLAARYGYTHQEV"
     gene            6301..6924
                     /locus_tag="PLD53_06630"
     CDS             6301..6924
                     /locus_tag="PLD53_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRC60623.1"
                     /translation="MDIAVFAKAPQAGAAKTRLIPALGASGAARLQRQLTLKALGLAK
                     RFCSTQITLWCSPDTHHHFFQALHTRFGLTLRSQSGTHLGTRMSNAFATHDGPLLLIG
                     TDCPVLETGHLVSAVSALREGHDAVFIPAEDGGYVLVGLRCPQPRLFEDIDWGSESVM
                     AQTRERLTELGLRWTEPATLWDVDRPSDLARLATLEGFADFAQRHNN"
     gene            6985..>7506
                     /locus_tag="PLD53_06635"
     CDS             6985..>7506
                     /locus_tag="PLD53_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HRC60624.1"
                     /translation="MKRLVLIGGGHAHVHVLDACARQPFPESDITLISPYPRQIYSGM
                     LPGWIAGHYSIEQCAIPIDLLARRAGIRFVQTACTDIDLESKQVRCANGQLIPFDLVS
                     IDSGPTVDLDHTPGASLHALPIRPIEEFVTEWPDLLQRARLQTPGFRQAIIGDGAAGT
                     ELAFAIAHRFSREK"
BASE COUNT         1593 a         2280 c         2051 g         1582 t
ORIGIN      
        1 cgagcagacc gatcaggaac agggcgagat agctggattc aggcatcgac aggcgagggc
       61 gaacggggaa ccgtaagggc ggcgcttgcc ttacggagcc tttcagagcc ctcgcaagga
      121 tcgcgaatcg atcaaaccac cgccaatcga tcgacggtgt cggggaactt ctcaaccagg
      181 cgaatcagca gtgcagcctg ggcgttcggc ttggccctcc cctgctccca attttccagt
      241 gtgcgtgggt tggtgcgcag ataccttgca aataccgggc gcgacaggtg aagacgctcg
      301 cgaagtgtca ccagctcatc ggcagtcacc tccggtaccg gttgtacctc aaccttgtgg
      361 gtgcgcagtg tctgcttgcc agcacgctca ctggccaagg catcaaagcc ctcggcaatt
      421 tccgcaaaca agttccgttt tttcatcgcc ttgcctccag ttctgctttc aacatcccct
      481 tgagcgtctt cttctcgtcg gcgctcaagt cctccatttc atccttgtca tacaaggtga
      541 atagccagaa ttgcttgcca ccatcccacc agtagtaaat tagtctcaat ccgccgctct
      601 tacccttacc gcgacgtgga tcgccgtgac gcagtttgcg aagaccgccc gtaccttcga
      661 tcacattgcc tacctcgggg tatttgagca tggcctgttg gagtattcga aatccttcat
      721 catccagata gtcggcacgg tacttggcga atgccggcag ttcgacgaac aatgctttca
      781 cttgatgaat atacgcaagt tacggtcctt tcgcaatatt gattcgtctg aaaatactga
      841 cattcgcaga cgagtctatt ttgcggaaac cgccggttcc gatcaaatgg acggcggcga
      901 ataccagcaa ttgttttgtt caagagagcg tcgactggca caacaaaaaa aacaggctcc
      961 gtggctgagc cgcggagcct gttctgaatc ggacaagttg tcgattactg gtagtacaaa
     1021 ccgccattga tgttcatggt cgcaccggtc atgaaaccgg cgagatcgga agccaggtag
     1081 gcacaagcgc caccaatttc ttccggcttg gccagacgct tcatcggcac gccgtcaatg
     1141 atgccttgca ggacttcagg cttgatcgcc atgaccatct tggtggcgac atagcccgga
     1201 gcgattacat tgaccgtaac gcccttggct gcaagttcgg ctgccagagc cttggaaaaa
     1261 ccgatcaccc ccgccttggc agcggagtag ttggtttgac cggcctgccc cttgacgcca
     1321 ttgagcgacg aaatgttgat gatgcggccc cagccacgct cggccatttt ggcggaaacc
     1381 tgcttggtca tgttgaacag gctggtcagg ttggtggcga tgacggcatc ccactggctc
     1441 aattccattt tggcgaacat gcggtcgcgc gtaatcccgg cgttgttaac cagaatgtcg
     1501 atcgggccgg ctttggcttc tgcctcggcg accatggccg cacacgagtc aaaactggaa
     1561 atatcccccg atacacagat gaaatccttg aagccggccg cagccatttc cttcgtccac
     1621 tcgtccggct tgtcgaactc cggatggtag gcggcaacca ctttgtgtcc cgccttggcg
     1681 agttcctgac aaattgaagt accgagaccg cccatggcac ctgtaacgaa agcaacgcgt
     1741 tgcgtcatgt tatttccttt caaaataagt aaatcaatga aacggctggt taaagccgct
     1801 gtcggtcttt agtacgaatg ctgaccaaca atgttgaagc gggtgcaact ctttgaattc
     1861 atctcctctg ctacatggta cacacaacaa gcgccatcct tgcggatgac aatacaacta
     1921 ggcacattcc tttacgtaac gccctggcgc cggctcaata ggcttgtatt tggcattgcc
     1981 aggttttttc cgcgcgccgc gctcgccctc tgaaaactgt ttcagccagt cactccagtc
     2041 gctccaccag ctgcctttgt gctcttgtgc gtcaagcagc cagttatccg cagtagtgcc
     2101 ctgctgggcg gtattcagcc agaagctgcg cttgttcttg ctcgccggat tgatcacccc
     2161 ggcgatatgg ccgctggcgc ccaggacaaa acgcgactcg ccacccagga gttgcgtacc
     2221 gagataagcg gctttccagg gcacgatgtg gtcttcacgg gccgccagaa cgtagttggg
     2281 agcctggacg cgccccagat cgaccttgac accgcacatt tccagcttct ccgggacgcg
     2341 cagagcgttt tccagataga gattgcgcag ataccagcag gcaaatggac cgggtaaatt
     2401 cgtgctgtca gaattccagt agagcagatc gaatgcctgc ggcgtgcgac ctttgaggta
     2461 attgctggcg acgtagttcc agaccaggtc attggcacga agcatcgaga aggcgctcga
     2521 caactcgcgt cccttcatga gtccgccctt gcccatttcc acttcccgtt ccgtgatcga
     2581 ctgttcgtcg atgaacaggc cgagttcacc ggcctcggag taatcaagca aggtggtcag
     2641 cagcgtcaga ctggccacct tgtcttctcc gcgagcagcg agcacggcca gcgcactggt
     2701 cagaatggtt ccgccaacgc accagcccag cacattgacc tgatcaaaat tgcagatttc
     2761 ccgaacgata ttgatcgcgg cgatcggccc attttccaga tagtcgtgcc agttcagatg
     2821 cccctgctcg tccttggggt tacgccagga aacaagaaat accgtattcc cctgctcaac
     2881 cgcatagcgg acaaaggaat tctcaggctg cagatcaagg acgtagaatt tgttgataca
     2941 cggcggtacg atcagcagcg gcctttgagc tgtcttggcg gtcgtcggcg cgtactgaat
     3001 caactggatg agttcattct cgaagatcac ggaaccggga gtcatcgcca ggtttccgcc
     3061 gacttcgaat gccgactcgt cggaaatgga tacgcgacct tttccaaaat caccaatcag
     3121 attattgatg ccgtccgtaa tgcacttccc ttgggtatcg agcgccagac gaatgaattc
     3181 ggggttggtc gcggcaaagt ttgctggcga caatgcatct atatattgac gcgccagaaa
     3241 gcgggcgcga tcctttgcct tgccatcgct ggcagggaac aactcgacca attcacggat
     3301 aaactgcgag ttgagcagat aggcctgccg gacatagtca taaatcgggc tgctacgcca
     3361 ttcgggcgac gcgaaccgac gatcacccgg atcggcttca atcaccgaca ccagcggctc
     3421 gtcctcctta cgaagcagca tgccgttcca gagttgctgg tgttgatcaa gaaaacgctt
     3481 ctgcagctgg gcaaagtgtt ctggatccgg gagctcctga gatgaacccg tgacacctgc
     3541 aacgctcgtc tgggaatcga gaaaatccat aattccctgc accaaggtcg cactgacctg
     3601 ggcgaacatc tgcgtcgcag gagcaaagga ttctgacgaa aatgccatgt gttttctcgt
     3661 gggtaccgtg gtgacgaccg aagtcaattg acgaactgcc agcaggcgat tttgcactgc
     3721 gacccaggca gtgtcaatgc ccgacgcgca ggcgacgaga tgaggaagcc gatgaatgcg
     3781 ggccagctcg tacccgaaac cccgcagtac cgtgcgtcac tcgactatcg cgcgcggggc
     3841 cgatgcgagt gataggccaa ttcgataccc caattcggcc accagcagga aatatgctcc
     3901 gggtattgtg atgccgtggt ataatcgtgc cctctcgtcg cgccgttcat cagtcttggc
     3961 gcccgtaagt tctggccagg cagtctcgcc tcgctcgatt cacaaatacc tgagtgtttg
     4021 cccagactgg ttcgcgtatt gacggttgtg gccggtcgtt ccgaccccct tcgttccgct
     4081 cgcgaatggc agacgcctgg agtcccccgc atgaaattta ccgaactggg tctcgtaccc
     4141 gagatccttc gttccattgc cgatcaaggc tatgaaaccc ccaccccgat tcaggctcag
     4201 gcaattcccg tcgtcctttc cgggcgcgac ctgatggcct gtgcgcaaac aggaaccggc
     4261 aaaactgccg gctttaccct gcccattctc aatcgattgg ccgcctccag ggtgagcggg
     4321 ccgcgcaaga tccgcgtgct gattctgaca ccgacacgcg aacttgcagc gcaggtcgaa
     4381 gaaagcgttc gcacctacgg cgcacacctg ccgttcaagt cactcgtcat ttttggtggc
     4441 gtcgggatga acccgcagat ttctgcgctc agaaacggcg tcgacatcct cgtcgcgaca
     4501 cccggccgtc tgctcgatca cgcacaacag cgaaatgtcg acctttcggg cgtcgaagtg
     4561 ctggtactcg acgaagccga tcgcatgctc gacatgggct tcatacacga tatcaaacgc
     4621 gtcatcgcac tggtcccgaa gaagcggcag aacctgctgt tttccgcgac tttctccgat
     4681 gagatccgca ccctggccgg cgccttcctg attcaacccg aatacgtaga atcggaacgt
     4741 cgcaacaccg cacccgaact ggtcaagcag tttgtctaca aggttgaccg cgaacgcaag
     4801 cgcgatctcc tcgtcgagtt gatccgtgac aaaggctggt accaggtgct cgtcttcacg
     4861 cgcaccaaat ggggcgccaa cggactggcc gacaaactgc tcaaggcagg catcgaggcc
     4921 gacgcaattc acggcaacaa aagccagaat gcgcgtacac gggcgctcgc caatttcaag
     4981 agcggcaaac tgcacgtact ggttgccacc gacatcgcgg cgcgtggtat cgacatcgac
     5041 gaattgccgc acgtcgtcaa ctacgagttg cccaacgtcg ctgaagacta tgtgcatcgc
     5101 atcggacgta ccggccgcgc gggcaccgaa ggttcggcca tctcgctggt ttgtgtcgac
     5161 gaacacaagc tcttgcacga tatcgagcgt ctgctcaaaa aacagatcga ggtgctggcg
     5221 caacccggct atgagcccga tcccagcatc cgtgccgaac caatcctgat gcgctcggcc
     5281 ggggcacgag gcggacagcg ttcgccaggc ggtcgaccgg gtggaggtgg tgggccgcgt
     5341 ggaaactccc caaaagcccc gggcggccac cgccccgcgg ccggcaaacc gggtggcggc
     5401 tcacaccgca gcggcagccg acaccgctaa cagtggttct agccgcgctt cccgagccag
     5461 gtggctccgg gcaagcgcgg acgagctggc atgtgcctat ccattgacgt cccgatcggg
     5521 acgttccaga cttgaagtcc tgattccaag tctgtcggtc gccaaatatc ccgaatcgtt
     5581 gccccgtgat ttccacgcct ctcctctcga tcatcatccc cgtgctcaat gaagcggctg
     5641 gaatcgtatc gcagctcgag gcgttgcagg ctttacgcaa cgccggcgcc attctgattc
     5701 tcgtcgacgg tggcagtagc gacaatacga cggaactggc caagccgctg gttgaccagt
     5761 tgatcgattc gtctcgtggc cgcgccgtgc agatgaacac cggtgcacag gccagcaaga
     5821 gtgaggtgct gctcttcttg catgccgaca cgagacttcc tccagcagcc gatgagcata
     5881 tccgcgaagc catcgccagc ggtgcgctgt gggggcgatt cgatatacgc atcgatgcgg
     5941 cccaccccct gttacggatc gttgaacgga tgatgaactg gcgctcgcgt ctgaccggaa
     6001 ttgccaccgg cgatcaggcc atttttgtcc ggcgagacct attcgaggaa actggaggct
     6061 atccggaact gcccctgatg gaagatatcg tcttcaccac ccgattgaaa caaattgcgc
     6121 ctcccgcatg cctgcggccg cgcgttatca cttcaggacg gcgctgggaa aagcacggtg
     6181 ctgcgcgcac catctttctg atgtggcgtt tgcgcaccga attctatttc ggtgctgacc
     6241 cggccaaatt ggctgcgcgc tacggctaca cacaccaaga agtgtgagct gggtgacctg
     6301 atggacattg cagttttcgc caaggcaccg caagcgggtg ccgctaaaac aaggctgata
     6361 ccggcgctgg gcgcctcggg ggcggcccgg ctgcaacgtc aacttacgct gaaagccctg
     6421 ggcctggcca agcgcttttg ttctacgcaa ataacgctgt ggtgctcacc cgatacgcat
     6481 catcattttt tccaggccct gcatacccgc tttggattaa cactgcgtag ccaatcgggc
     6541 acgcatcttg gaacacgcat gtcgaacgca tttgctactc acgacggccc tttgctgctc
     6601 atcggaaccg actgcccggt gctggaaacc ggacacctcg tcagcgcggt cagcgcgctg
     6661 cgcgaaggcc acgatgccgt cttcattccg gcggaggatg gcggctatgt gcttgtcggg
     6721 ctacgatgcc cgcaaccccg cctgtttgaa gacatcgact ggggaagcga atcggtcatg
     6781 gctcagacac gcgaacgtct gaccgagctt ggcctgcgct ggacggaacc cgctaccttg
     6841 tgggatgttg atcgaccatc cgatctggcg cgtctcgcca cacttgaagg ctttgcagac
     6901 tttgcgcaac gtcacaacaa ttgatggcaa caccagcgaa cggctcgaat cccgttcctg
     6961 cgacctacgc cggaaggccc ggccgtgaag cgtctggtgc tcatcggcgg cggtcatgct
     7021 cacgtgcacg ttctggatgc atgtgcccga caaccgtttc ccgagagcga tatcacgctg
     7081 atctcaccct atccacggca aatctattcc ggcatgttgc ctggctggat cgccggccat
     7141 tacagcatcg agcaatgcgc aatcccgatc gaccttcttg cccggcgcgc cggtatccgc
     7201 tttgtgcaga ccgcctgtac agatattgac ctcgaatcga aacaggtgcg ctgcgccaac
     7261 ggtcaattga tcccgtttga ccttgtttcc atcgacagcg gtccaaccgt cgatctcgat
     7321 cacacgccgg gcgcatcgct acacgcacta cccatccgtc ctatcgagga gtttgtcact
     7381 gaatggcccg acctgctgca gcgcgcccgc ctccagaccc caggctttcg gcaggccatc
     7441 atcggcgatg gcgctgcggg tacggaactg gcctttgcca tcgcgcatcg attctctcgt
     7501 gaaaaa
//
LOCUS       DAORPM010000107         7495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2494_length_7495_cov_8.162097, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000107 DAORPM010000000
VERSION     DAORPM010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_7495_cov_8.162097"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PLD53_06640"
     CDS             <1..869
                     /locus_tag="PLD53_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRC60625.1"
                     /translation="LVPLARDVDESLRLAASLEQGSEHPLGRAILARAQTLSATLSTL
                     ADFQSTAGKGVSGRVDGRILRLGSPDWFADLALPIEQIAALQAAGKTVVVLAEDDVPL
                     ALLAIADSLRDTSRAAVARLKEMGIRVVMLTGDNAATAAAIAAEAGIDEFRAGILPGD
                     KAAAVGALRTGGGVVAMVGDGVNDAPALAAADVSFAIGAGSDAAIEVADVTLVRSDLQ
                     GVADAILLSRATLGKIRQNLFLAFIYNVAGIPLAALGMLNPVFAGAAMAMSSVSVVSN
                     SLLLRRWRPGER"
     gene            919..3777
                     /locus_tag="PLD53_06645"
     CDS             919..3777
                     /locus_tag="PLD53_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC60626.1"
                     /translation="MTLTGSLSLLDELLASSEHIAYRLDFVRGGYDFISPRAANLLGI
                     ALADLHARGWELLDEHWVGDDRERCFAELVSLCAAAPGRSLSTRLEYRLRDAAGNMTW
                     YCDSMTVLSDISGRPLTACGIAVDVTLQREQEKALRDSEEKYRATMDAAQVGVFILQD
                     YKFSYVNPKLLAMFGYRDKELLNTHGPQALVIAEQHEFLIGKMRRRGAGEVSEPYELT
                     GLRKDGSTFPMMVMGRPWPIGGRPASVGTLIDLSAQRQIEQALKESSNRYAALFEGAQ
                     DAIVVADIDTGHLIDANVEAELLFLRPRENLIGMSFAALHPPALTAAYKEAYTRHIRA
                     GRGGPDEMQIVTADGDIVDVEISTNVIETAGRQRLIQGVFRNITQRKLTEAQLSLAAR
                     VFESSQEAIIITDARAVIISVNPALSGMTGYAAEEVIGRMLPFLGSDRQSAEFYSTMW
                     KTLNDEGRWQGEVWNKRKNGEIYPVELTISVYRGASGRVLNYVGIESDISERLAAQEN
                     IRQLAYYDPLTRLPNRTLLQDRVEQALAAAEREGKQVAVCFIDLDHFKVINDSLGHSA
                     GDQILVQVSQRLLDCIRRRDTVARLGGDEFVVLLSEITPDSAAEVARKILAAVARPFT
                     VERHELSVTPSLGISLFPQDGRDFESLLKYADVAMYRAKESGRNAFQFFASDMNIAAL
                     ERLVLENRLRQGLERGEFILHYQPQINVATGRIVGAEALIRWQHPEIGTVSPGKFIPV
                     AELSGLIGPLGDWVLREACRQNRAWQIAGLPSICIAVNVSSVQFRGGRLEESVREVLA
                     ETGLPAECLELELTEGTLMSDAKATVDALQRLSAMNIRLAIDDFGTGYSSLSYLKRFP
                     IDRLKIDQSFVRDIVTDPDDWAIASAIISMGHSLRLKVIAEGVEHANQLEMLAHQGCD
                     EVQGYHLSFPLPAEEFAELLQQQTFASQVRQQSFDMGD"
     gene            3780..3986
                     /locus_tag="PLD53_06650"
     CDS             3780..3986
                     /locus_tag="PLD53_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRC60627.1"
                     /translation="MARIVIGIGGMSCQGCVKNVTGVLRDLTGVESVEVSLDAGQASI
                     DYDPAIADVAQFKAAVEAAGFDAL"
     gene            4037..5140
                     /gene="selD"
                     /locus_tag="PLD53_06655"
     CDS             4037..5140
                     /gene="selD"
                     /locus_tag="PLD53_06655"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRC60628.1"
                     /translation="MSTTSDEISPTSEPSEASVPVGPIRLTQLSHGGGCGCKIAPGVL
                     EQILARVSPGLVPPQLLVGRDTRDDAAVYQINDEQAIVATTDFFMPIVDDPFDFGRIA
                     ATNAISDIYAMGGTPLFALAIVGMPVNTLPLETIGRILEGGESVCAKARIPIAGGHSI
                     DSVEPIYGLVVIGLVHPGNIRRNSGAQVGDKLILGKRLGIGLYSAAFKKGMLSPHDYE
                     LMIELTTKLNTPGPMLACLAGVHAMTDVTGFGLLGHLLEVCNGSGVSARLDYSAIPVL
                     PRAGDLADRGCVTGASARNWAACGDSVELDSKRFGNSERALLTDPQTSGGLLISCAPE
                     VVTQVLAVFLQQSCDHAAVIGEIVEGKPGVRFI"
     gene            complement(5191..5892)
                     /locus_tag="PLD53_06660"
     CDS             complement(5191..5892)
                     /locus_tag="PLD53_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRC60629.1"
                     /translation="MVHSSVTEEIQRELETRLLNGSWTVGTRLPAERILAETLGVSRN
                     SLRVAIFSLKARGLLVSKWGSGVFVTDRLQGIISSPWRQLVADHPDLRWDTLEFRREL
                     EGATAHYAALRADKSDLKKIEGIIKRLNKAYDTGATREEHKADADFHEAIAEASHNSM
                     FLYLHAGILRLLREHISLNLQALEDPSGKVTNQLRQQHLDIWHAIRRRQPDAARQAML
                     AHIDFTRSELASRDI"
     gene            6102..>7495
                     /locus_tag="PLD53_06665"
     CDS             6102..>7495
                     /locus_tag="PLD53_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC60630.1"
                     /translation="MNALISALRRRFPAERVITDELRRLAWGTDASFYRLVPRVVVVV
                     ENEADVAHVLAVARQEDIHLTFRAAGTSLSGQAITDGILVLIGEGFSGFELAADALSV
                     SLGPGIIGSEVNRRLAPFGRKIGPDPASIATCKIGGIAANNASGMCCGTAQNSYQTLV
                     GLRVMLADGSVLDTEDAVSLTAFQQSHAELLGELERLGNETRADDLLAARIRHKFKIK
                     NTTGYSLNALADYTNPIDILAHLMIGSEGTLGFISRITYRTVAEDPCKASALVFFPDI
                     ETACQAVIRLKPQPVSAVELLDRAALRSVEDKAGLPPMIRSLGESVAALLIEVRDQES
                     VGLQARIDAALSAINGIATVEPALFSTDPETGEMYWKVRKGTFPSVGAMRRTGTTVII
                     EDVAFPIESLAAATLDLQALLRHHGYHEAIIFGHALEGNLHFVFTQDFGDPAEVARYA
                     GFMDEVCKLVVDKY"
BASE COUNT         1434 a         2104 c         2338 g         1619 t
ORIGIN      
        1 atcttgtgcc actggccagg gacgtggacg agtcactgcg gctggcggcc tccctggagc
       61 agggctccga gcatccactc ggacgcgcca tcctggcacg ggcgcagacc ttgtccgcga
      121 cactgtcaac cctggccgat tttcagtcga ctgccggaaa gggcgtttca ggcagagttg
      181 acggacgcat cctgcggctg ggttcccccg actggtttgc cgacctcgcg ctgccgatcg
      241 agcagatcgc tgcgttgcaa gctgcgggca agacggtggt tgtgctcgct gaagacgacg
      301 tgcccttagc cctgctggca attgccgatt ccttgcgcga tacctcacgc gcggcagtcg
      361 cgcgtctgaa ggaaatgggg atccgtgtcg tgatgttgac cggcgataac gcggcgacgg
      421 ctgcagcgat cgctgccgaa gccggaatcg acgaatttcg tgccggcata ttgcctggcg
      481 acaaagctgc cgcggtcggt gcgttgagga ccggcggcgg ggttgtggcg atggtcggcg
      541 atggagtcaa cgacgcaccg gccttggctg ctgccgatgt cagttttgca atcggcgccg
      601 ggtcggatgc cgccatcgaa gtggccgatg tgacgctcgt gcgcagcgat ctgcagggtg
      661 ttgccgatgc catattgctc tcaagggcga cgctcggaaa aatccgacag aatcttttct
      721 tagccttcat ttacaatgtc gctggaatac ccttggccgc gttgggtatg ctcaatccgg
      781 tatttgccgg tgcggcaatg gccatgagtt cggtttcggt cgtgtcgaac tcgctgttgc
      841 tccgtcgctg gcgaccgggc gaacgatgat aatgttcatg atattgagcg aagccgcatt
      901 aatgatatga gtggtgcatt gactttgacc ggaagcctat ccctgctcga cgaactgctt
      961 gcatcaagcg agcatatcgc ttaccggctc gattttgttc ggggcggcta cgacttcatc
     1021 agtccgcgtg cggcgaacct gcttggtatt gcacttgccg acctgcatgc acggggctgg
     1081 gaactgcttg atgagcattg ggtcggagat gaccgcgagc gctgttttgc cgagcttgtc
     1141 tcattatgtg cggcggcacc tggccgttcc ctgtcaacgc gtctcgaata ccgcctgcgc
     1201 gatgcggccg gcaacatgac ctggtattgc gattcgatga cggtgctttc ggacatttcg
     1261 ggccggcctc tcaccgcctg cggaattgcc gtggatgtga cgctccagcg cgaacaggag
     1321 aaggcgctgc gcgacagcga ggagaaatac cgggcgacta tggatgccgc ccaggtcggc
     1381 gttttcatcc tgcaggacta taaattcagt tacgtcaatc cgaaactgct tgcgatgttc
     1441 ggttaccgcg ataaagaact cctcaacacg cacgggccgc aggctttggt gatcgccgag
     1501 caacacgagt tcttgatcgg caagatgcga cggcgcggtg caggtgaggt gagcgagccc
     1561 tacgaactga ccggtttgcg caaggatggt agtacctttc cgatgatggt catgggtcgt
     1621 ccgtggccca tcggcgggcg cccggcgtcg gtcggtacct tgatcgacct gagcgcgcag
     1681 cggcagatcg aacaagccct gaaagagtcg agcaaccggt atgccgcact cttcgagggc
     1741 gcgcaggacg ccattgtggt ggccgacatc gataccgggc acttgatcga cgccaatgtc
     1801 gaggccgagc tcctgttcct gcggccgcgc gaaaacctga tcggcatgtc gtttgcggcg
     1861 ctgcatcctc ccgccctgac agccgcctac aaggaggctt acacacggca cattcgcgcc
     1921 gggcgaggcg gcccggacga gatgcagatt gtcacggccg atggggacat cgttgacgtc
     1981 gagatcagta ccaacgtgat tgaaacggcg ggccggcagc gcctgattca gggcgtcttt
     2041 cgtaacatca cgcagcgcaa gctgaccgag gcgcagctca gtctcgccgc tcgggttttc
     2101 gagagcagcc aggaggcgat catcatcacc gacgctcgcg cggtcatcat ttcggtgaac
     2161 cctgctttgt ccggaatgac cggttatgct gcggaggagg tgatcggccg catgttgcct
     2221 tttctcggct cggatcgaca gtccgccgaa ttttactcga cgatgtggaa aacgctcaac
     2281 gacgaaggtc gctggcaggg tgaagtctgg aacaagcgca agaacggcga aatctatccg
     2341 gtagaactga ccatcagcgt ttatcgcggc gcctctggca gagtgctcaa ctatgtcggt
     2401 atcgagtcgg atatttccga gcgccttgcc gcacaggaga acattcgcca actggcttac
     2461 tacgatccgc tgacccgact gcccaaccgc acgctgttgc aagatcgcgt cgagcaggcg
     2521 ttggccgcgg ccgagcgtga aggcaagcag gtcgccgtgt gctttatcga tctggatcac
     2581 ttcaaggtta tcaacgactc gctcgggcac tctgccggcg accagatact cgtccaggtg
     2641 tcgcaacgtt tgcttgactg cattcgacgc agggataccg tggcgcgtct cggtggcgac
     2701 gagttcgttg ttctgctgag tgaaatcacg cctgacagcg ccgccgaagt ggcgcgcaag
     2761 atactcgctg cggttgcccg tccgtttacc gtcgagcgac acgaactgag cgtgacgccg
     2821 tcgctgggca tcagcctctt tcctcaggat ggcagggatt tcgagtcgct gctcaaatac
     2881 gccgatgtgg caatgtatcg ggccaaggaa tccgggcgaa acgccttcca gttttttgcc
     2941 agcgatatga atatcgcagc acttgaacgg ctggtgctcg aaaacagact tcgccaaggc
     3001 ctcgagcgtg gcgagttcat tctccattac cagccgcaaa tcaatgtggc taccggacgc
     3061 atcgtcggtg ccgaagcgct gattcgctgg cagcatccgg agatcggtac ggtttcgccc
     3121 ggcaagttca tcccggtggc cgaattgtcc ggactgatcg ggccgctggg cgattgggtg
     3181 ctgcgcgagg cgtgtcggca gaatcgcgcg tggcagatcg cgggattgcc gtcgatctgt
     3241 atcgccgtca atgtttcttc ggtgcaattc cgcggcggca ggcttgaaga aagtgttcgc
     3301 gaagtcctcg ccgaaaccgg gctgcctgcc gagtgtctcg aactcgaact gaccgaaggc
     3361 accctgatga gcgatgccaa ggctacggtc gatgccttgc agcgactatc ggcgatgaac
     3421 attcggctgg ccatcgacga tttcggcacc gggtattcga gcctgtcgta tctcaagcgc
     3481 tttccgatcg atcggctgaa gatcgatcag tcgttcgtgc gtgatattgt caccgacccg
     3541 gacgattggg cgatcgcctc ggcgattatc agcatggggc acagcctgcg cctcaaggtc
     3601 atcgccgagg gcgtcgagca tgccaatcaa ctggaaatgc ttgcgcatca aggctgcgat
     3661 gaagttcagg ggtatcattt gagctttcca ttgcccgccg aagagtttgc cgagctattg
     3721 cagcagcaga ctttcgccag ccaggtgcgg caacagtcat ttgacatggg agattgagca
     3781 tggcgcgtat tgtcatcgga attggcggca tgagttgtca gggttgtgtc aagaatgtca
     3841 cgggggtgtt gcgggacttg acaggggtgg agtcggtcga agtctcgctg gacgccgggc
     3901 aggccagcat tgactatgat ccggcgatcg ccgacgtcgc acaattcaag gccgcggttg
     3961 aggccgcggg attcgacgct ctttgaagca gggcgcgcca aaagatcaat cagttccagt
     4021 ttccaagaag aagagaatga gtacgacaag cgatgagatt tccccaactt cggaaccttc
     4081 cgaggcatcc gttccggttg ggccgatcag actgacccag ttatcgcacg gcgggggatg
     4141 cggatgcaag atcgctcctg gcgtgctcga acagatcctc gcaagagtct cgcctggact
     4201 ggtgccgccg caattgctgg tgggacgcga tacccgcgac gatgcggcgg tgtaccagat
     4261 caacgatgag caggcgattg ttgcgacgac cgattttttt atgccgattg tcgatgatcc
     4321 cttcgatttc ggccgtatcg ccgcaaccaa cgccatttct gatatttacg ccatgggtgg
     4381 aacgccgctg tttgctctgg cgattgtcgg aatgccggtg aatacgctgc cgctggaaac
     4441 catcgggcgc attcttgaag gaggcgagtc cgtttgcgcc aaggcgcgta ttccgattgc
     4501 cggcggccac agcattgatt cggttgagcc gatctacggc ctggtggtca tcggcctggt
     4561 gcaccccggg aacatcaggc gcaacagcgg tgcacaagtc ggggacaagt tgattctcgg
     4621 caaacgtctg ggtatcggac tgtatagcgc cgccttcaag aaaggcatgt tgtcgccgca
     4681 cgattatgag ctgatgatcg agttgaccac gaagctcaat acgcccggcc cgatgttggc
     4741 atgtcttgcc ggggtgcacg ccatgaccga cgtgaccggc tttggattgc tgggccacct
     4801 gctggaagtc tgtaacggtt ccggcgtgtc ggcgcggcta gactattcgg caatccctgt
     4861 tttgccgagg gccggtgatc ttgccgatcg gggctgcgtg accggagcgt ccgcccgcaa
     4921 ctgggccgcc tgtggcgaca gcgtcgagct tgactccaag cgattcggaa acagcgagcg
     4981 cgcgttgctc accgatccgc agaccagcgg aggcctgctc atttcctgtg caccggaggt
     5041 cgtcactcaa gtgcttgcgg tttttctgca acagagctgc gatcatgcgg cggtgatcgg
     5101 cgaaatcgtt gaagggaaac ccggggtcag atttatctag tcaatcaagc aagaccgggc
     5161 aagtgctcca atgcgctgac tgcacgttga ctagatgtct ctgctggcca attccgagcg
     5221 cgtgaaatcg atgtgagcga gcatcgcctg ccgtgccgca tccggttggc gtcggcgaat
     5281 ggcgtgccag atgtcgaggt gttgctggcg caattgattc gttaccttgc cgctggggtc
     5341 ttccaaggct tgcagattga ggctgatgtg ctcacgcaac aggcgcagga tgccggcgtg
     5401 caggtagaga aacatgctgt tgtgcgacgc ttcggcgatg gcttcgtgaa agtcggcgtc
     5461 tgccttgtgt tcttcgcgcg tcgctcccgt gtcgtaggcc ttgttcaggc gtttgatgat
     5521 gccttcgatc ttcttgagat cgctcttgtc agcacgcaag gcggcatagt gtgcggtggc
     5581 gccttccagt tcgcggcgaa attccagcgt atcccatcgc agatccggat ggtcagccac
     5641 cagttgccgc cagggtgatg aaatgatccc ctgcaggcgg tcggtaacga atacgccgct
     5701 tccccatttg ctgacgagca ggccacgggc tttcaggctg aaaatcgcaa ctcgcaagga
     5761 gttacgggaa acccccagcg tttccgccag aatccgctcg gccggcaagc gcgtgccgac
     5821 tgtccaggag ccattgagca ggcgggtttc aagctcgcgt tgaatctctt ctgtaacgct
     5881 gctatgtacc atagcgagtt tcccgaagtc cttcgtattg gcgggtgtgg cgccgaaaaa
     5941 aatattggca ctaccaattt tccggtcatt gtagctttaa ttatgattcg tgccgtcaac
     6001 cgttactgat gcaatgtgtt ggccattggt taattggccg cagtgtgctt atgaaaaccg
     6061 ttatcatcca ttgtgttata ggatagggga gcaggcggaa attgaacgcg cttatttctg
     6121 cacttcgcag gcgtttcccg gcagagcgcg tcatcactga tgaactgcga cggctggcct
     6181 ggggtactga tgccagtttc taccgactgg ttccacgggt cgtggttgtt gtggaaaatg
     6241 aggccgacgt ggcgcacgtg ctggctgtgg ctcgccagga ggatattcac cttaccttcc
     6301 gtgccgcagg caccagcctg tccgggcagg cgattaccga cggtatcctg gtcctgatcg
     6361 gagaaggctt ttccggcttt gagctggctg ccgatgcact cagcgtaagt ctcgggccgg
     6421 ggatcatcgg cagcgaagtc aatcggcgcc ttgccccctt cgggcgaaag atcggtcccg
     6481 atccggcatc gattgccacc tgcaagatcg gcggcattgc cgccaacaac gcctccggca
     6541 tgtgttgcgg cacggcccag aacagctacc agacgctcgt cgggctgcgc gtcatgctgg
     6601 ccgacggcag cgtgctggac accgaggatg cggtcagcct gaccgcattt cagcaaagcc
     6661 atgccgaact tcttggcgag cttgaacgtc tgggaaatga aactcgtgcc gacgaccttc
     6721 tggccgctcg tatccggcac aagttcaaga tcaagaacac cacgggctac agcctcaatg
     6781 cgctggcgga ttacacgaac ccgatcgata ttctggcgca tctgatgatc ggctcggaag
     6841 gaacgttggg cttcatttcc cggatcacct accgcaccgt cgcggaagat ccatgtaagg
     6901 ccagtgcgct ggttttcttt ccggatatag aaacggcgtg tcaggcggtg attcgcctca
     6961 aaccgcagcc ggtctccgca gttgagctgc tggatcgagc cgcactgcgc tccgttgaag
     7021 acaaggccgg cctgccgccg atgattcgtt cgctgggcga atcagtcgct gccctgctga
     7081 tcgaagtccg cgaccaggaa agcgtcggct tgcaggcgcg catcgatgcg gcgctttcgg
     7141 ccatcaacgg tatcgctacc gttgagcctg cgttgttttc cactgacccg gaaaccggcg
     7201 agatgtactg gaaagtgcgc aaggggacgt ttccttcagt cggagccatg cggcggacag
     7261 gcaccacggt catcatcgag gatgtggctt ttccgatcga atcgctggca gcggccacac
     7321 ttgatctgca ggcgctgctc agacaccacg gctaccacga ggcgatcatc tttggacatg
     7381 cgctggaggg caatctgcat ttcgtgttta cccaggattt tggcgatccg gccgaagttg
     7441 cacggtacgc gggcttcatg gacgaagtct gcaagctggt cgtcgacaag tacga
//
LOCUS       DAORPM010000108         7444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2528_length_7444_cov_8.742861, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000108 DAORPM010000000
VERSION     DAORPM010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_7444_cov_8.742861"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..176
                     /locus_tag="PLD53_06670"
     CDS             6..176
                     /locus_tag="PLD53_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC60631.1"
                     /translation="MVSYNPAQAAGLIDRGEISAGKRADLVAVAHVNGLVQASVVFSA
                     GKRVFEARFDHG"
     gene            169..741
                     /gene="phnN"
                     /locus_tag="PLD53_06675"
     CDS             169..741
                     /gene="phnN"
                     /locus_tag="PLD53_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HRC60632.1"
                     /translation="MAEGTLFYVVGASGSGKDSLMRYGRQRLAGDSNVIFAHRYITRP
                     VELHGENHVALTDGEFDARLAAGFFAMNWSSHGLRYGIGREINLWLAKGCNVVMNGSR
                     AYLIEARRSYPALIAVLVTVSPEILAARLKARARETDAQIVGRLLRAPQFMPPVGNVE
                     IIQNDGDLCQAGENFVQVISASTASSRAIA"
     gene            672..1523
                     /gene="phnP"
                     /locus_tag="PLD53_06680"
     CDS             672..1523
                     /gene="phnP"
                     /locus_tag="PLD53_06680"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: protein motif:HMM:TIGR03307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HRC60633.1"
                     /translation="MPGRGEFRAGDFCVYGVFESDRLIMRVTFLGTGAAGGIPVYGCN
                     CFVCTRARAVLDFRRSPCSGMVETGNTRLLIDAGLTDLTERFPPGNLSAIVLTHFHPD
                     HVQGLFNLRWGIGDRIPVFAPPDSEGCADLYKNHGLLEFHQLAKFNAVAIGDLILTPL
                     PLIHSKPTFGYAIEDSYGQRFAYLSDTAGLPVETEQFLMDWRPQSVALDCTHPPQTKY
                     PSNHNDVALALDIAETLTPADVWLTHIGHEMDGWLVKNVGDLPSRVHVASDSRVLPDI
                     DVSNAVL"
     gene            1873..4023
                     /gene="ovoA"
                     /locus_tag="PLD53_06685"
     CDS             1873..4023
                     /gene="ovoA"
                     /locus_tag="PLD53_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HRC60634.1"
                     /translation="MPNPVFPRTPNLSGKDAEKKRVEILDYFHATFDRYEQLFEALNC
                     DEAYYEKPIALRHPLIFYLGHTATFFVNKLLLAGLIEQRINPKFESMFAVGVDEMSWD
                     DLNDARYEWPSVSEVKAYRDSVRAMGDQLIRTTPLSLPIGWDHPWWIILMGIEHERIH
                     LETSSVLIRQHRLKYLAPHPAWPPCRATSAAPQNDLLEIPATQLRVGRDANGNIDPAG
                     VAYYGWDNEFGCHETSITAFQASRYLVSNQEFLGFVEAGGYADESLWLDEGAGWLKFT
                     RAEHPTFWIRDGRQWRLRLMLEEVPMPWDWPVETNYHEAKAFCNWKARTSGDPVRLPT
                     EDEWQALREFASIDDLACGAEERSSGCAVPANLHLARYASSCPVTEFAHGPLFDVIGN
                     VWQWTETPIYPFDGFQVHAVYDDFTTPTYDGRHNIIKGGSWISCGNEALPASRYAFRR
                     HFFQHAGFRYVKSGAPATSQTSQYETDRLVSEYAEFHYGDEYFDVPNFPRALAQLAIA
                     AMGEKPADKALDLGCATGRATFELARHFERVTGIDFSARFIGLGTRLAEQGRLRYTLV
                     EEGELVAFKERSLAELGLGPVAQKVEFFQGDACNLKPVFTGYDLILAANLIDRLYDPE
                     KFLESVHERLNPGGVLLLASPYTWLPEHTKREAWIGGFKKDGENFSTFDGLKAILGKH
                     FRLLRGPFSVPFVIRETKRKFQHTLSEVSIWERN"
     gene            complement(4051..4383)
                     /locus_tag="PLD53_06690"
     CDS             complement(4051..4383)
                     /locus_tag="PLD53_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRC60635.1"
                     /translation="MERHLSSSAAEPTRAEIEALQEPAVIEFGASWCGYCIAAQPLIA
                     SALGDHPRTRRIKIEDGPGRRLGRSFGVKLWPTLVFMRRGQEVARLIRPTDRSAIAQA
                     LTQIDDES"
     gene            complement(4408..5319)
                     /locus_tag="PLD53_06695"
     CDS             complement(4408..5319)
                     /locus_tag="PLD53_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRC60636.1"
                     /translation="MKTNEEQDQEQIQEPEPDPTVDKFGEALNATRFRMLEDIARELA
                     GDVVFPTYFDAALRLRKELQNPDLPTARIAKIVGIEPLTAAKLMQLANSAYYSRDGSK
                     AKDLRAAINRLGVDVVRTTALAIAMAQLLHSKDMAIFGNLAGALWDHSIRTAAAARIL
                     ARTYTRINPDEALLAGLVHDLGAFYMLYRAVQYPELLLRPDTVKYIIMRWHEGVGVTL
                     LNALGMPEEIVNATIDHDQPRPISTTVRTLSDIVYVGNIIAGTHFEWSRQDTDPDAGD
                     AGSVRQSFKDLLPEVEADTMEMLSVFK"
     gene            complement(5439..6527)
                     /locus_tag="PLD53_06700"
     CDS             complement(5439..6527)
                     /locus_tag="PLD53_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HRC60637.1"
                     /translation="MDRRAFLLALASLSTWRAGVAQADVETVEFAPVLPGRKLTFPVD
                     HGAHPDFRTEWWYATGWLNLPDGTPLGFQSTFFRVRTGIGEDNPSAFAPRQLILAHAA
                     LADPRLGRLRHEQRAARVGFGRSGFETGRTNVWIGDWRLEQREGGYQAEVRGEDFSYA
                     LALMPDGPPMLNGDAGFSAKSPQAGHASYYYSRPQLKVSGWVTLNGQRQEVTGLAWLD
                     HEWSSELLHASAQGWDWIGLNLTDGSALMAFRLRGKDGTPLWSAATFRQPGSRVQALP
                     PEVIGFTSVRRWRSPRTAISYPVEWRVRISERQLHLIPLIDDQELDSRRTTSAIYWEG
                     AVRVEEGGSEIGRGYLEMTGYGEKIRLG"
     gene            complement(6527..>7444)
                     /locus_tag="PLD53_06705"
     CDS             complement(6527..>7444)
                     /locus_tag="PLD53_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60638.1"
                     /translation="LQARIAAVTGVGRVNFTRHDRLRLVAGQTPVALIARPVSADGRE
                     LPLVGTAVLDGSSKEPAIWISEALQDIYGWQPGQQVELPLAGRPVRMRVAGVWRDYAR
                     QTGALMIDLADYRRLTGDQLVNDAAILLAPGVAPDQVAAALTATSSPGTLSIAYPGEI
                     RAISLRIFDRTFAVTYLLEAVAVIIGLAGVAASFAALAVARRREFGMLRHLGLTRRQI
                     GWMLAQEGALAAGVGVIGGLLAGAAIGLVLIEVVNRQSFHWSMDLRIPYGSLAVFASV
                     LIVLAALAAVAAGRQAMRQGAILAVREDW"
BASE COUNT         1486 a         2231 c         2199 g         1528 t
ORIGIN      
        1 tgcgactggt cagctacaat ccagcccagg ctgccggact gatcgatcgt ggagaaatct
       61 ctgccggcaa gcgagccgat ctcgtggctg tcgcacatgt caacggactg gttcaggcga
      121 gtgtcgtttt ctcggccggc aaacgggtat tcgaggcgcg tttcgatcat ggctgaggga
      181 acgcttttct acgtcgttgg tgcttccggc agcggcaagg acagcctgat gcgttatggc
      241 cgtcagcgtt tggcagggga ttccaatgtt atcttcgcgc atcgctacat tacacgccca
      301 gttgaactgc acggagagaa tcacgtcgcc ctgaccgatg gcgagtttga tgcccgtcta
      361 gccgccggct tcttcgccat gaattggagc agccacggtt tgcgctacgg aattggccgg
      421 gagatcaact tgtggctggc caagggatgc aacgttgtga tgaatggatc gcgcgcgtat
      481 ctgattgaag cacgacgcag ttatccggca ttgattgcag tcctggttac cgtctcaccc
      541 gaaattctgg ccgcaagact gaaagcgcgg gctcgtgaga ccgacgcaca gattgtcgga
      601 agattgttgc gcgcgcctca gttcatgcct cccgtcggca atgtggaaat catccagaac
      661 gatggtgatc tatgccaggc aggggagaat ttcgtgcagg tgatttctgc gtctacggcg
      721 tcttcgagag cgatcgcctg atcatgcgcg tgacgtttct cggcacaggt gcagcgggag
      781 gcataccggt ctacgggtgt aactgtttcg tctgtacgcg ggcacgggcg gttctggact
      841 ttcgccgcag cccttgctcc gggatggtgg aaacaggcaa cacccggtta ttgatcgacg
      901 ccggtttgac cgatctcacc gaacggtttc ctccgggaaa tctgtcggcg attgtattga
      961 ctcatttcca tcccgatcat gtccagggtc tgttcaattt acggtgggga atcggcgatc
     1021 gaataccggt tttcgcaccg ccggattctg aagggtgtgc tgacctttac aagaatcacg
     1081 gcctcctcga atttcaccag cttgccaagt tcaacgcagt cgcaatcggc gacctgatac
     1141 tgacaccatt gcccctgatt cattccaagc cgacctttgg ctatgcgatt gaagattcct
     1201 atggtcaacg ttttgcctat ctgtccgaca cagccggact gccagtggaa acggagcagt
     1261 tcttgatgga ttggcgtcct caatcagtcg ccctggactg cactcatccg ccacagacaa
     1321 agtacccgag caaccacaac gatgtggccc tggccctcga tatagcagaa actctaactc
     1381 ccgccgacgt ctggttgacc cacatcggtc acgagatgga tgggtggcta gtgaagaacg
     1441 tcggcgatct gccctcgcgc gtccatgttg ccagtgattc acgcgttttg ccggatattg
     1501 acgttagtaa cgcagtgctc tgattgctga ggcagccgga tctctcgcgg tgatattcgc
     1561 cggattcagg aattcaggat tctcaagaag tagttccggt cgactccttt cggcgaagcg
     1621 acaaggcccc acagcatagc tggctttgcc cggcttccgt acctcaggca tgacggagag
     1681 ggcggcaata gcgatccgtg catttcatga atatgacatt caatgcgggc gcactgaatg
     1741 ctgatacatg gaagaaaccg gtcaggtcgt gcggggcggt ctacggttca gtagccgcca
     1801 agcgttagaa tgtgccgatt gaagaacttc gatcaactct tttacaaccg cctgttgaaa
     1861 acccgaatcc gtatgccaaa cccagtcttt ccaagaacgc ccaatttgtc agggaaggat
     1921 gccgagaaga aacgagtcga gattctcgac tattttcacg cgacgtttga tcgctacgaa
     1981 caactgttcg aggcgttgaa ctgcgacgag gcctactacg aaaagcccat tgccttgcgt
     2041 catccgctga tcttttacct cggccatacc gcgacctttt tcgttaacaa gttgctcttg
     2101 gccggcctga tcgagcagcg catcaatccg aaattcgagt cgatgttcgc cgtcggggtc
     2161 gatgaaatga gttgggatga cctcaacgat gcgcgttacg aatggcccag tgtaagcgag
     2221 gtcaaagcct atcgcgattc agtgcgtgcc atgggtgacc agctgattcg aaccacgccg
     2281 ttatcgttgc cgatcggctg ggaccatccg tggtggatca tcctgatggg aatcgagcac
     2341 gagcgcattc atctggaaac ctcttccgtg ctgattcgtc agcatcgcct gaagtacctc
     2401 gcaccgcatc ccgcctggcc gccctgtcgt gcaacttctg cggcaccgca gaacgatttg
     2461 cttgaaatcc cggcgaccca attgcgcgtc ggaagggatg ccaatggcaa catcgatcca
     2521 gcgggtgttg cctattacgg ctgggacaac gagttcggat gtcacgagac ttccatcaca
     2581 gcctttcagg cgagccgcta tctggtttcc aatcaggagt ttctgggatt cgtcgaggcc
     2641 ggaggctatg ccgacgaatc gctctggctg gatgagggtg caggctggct caagtttacg
     2701 cgcgccgaac accctacctt ctggatccgc gatggcaggc aatggcgctt gcgtctgatg
     2761 ctcgaagaag ttccgatgcc ctgggactgg cccgtggaaa ccaattacca tgaggccaag
     2821 gcgttctgta actggaaagc ccgtacctct ggcgatccgg tgcgtttgcc gaccgaggac
     2881 gagtggcagg cattgcgcga attcgccagc atagacgacc tggcctgtgg tgcggaagag
     2941 cgcagttccg ggtgcgctgt cccggcaaac cttcacctcg cccgttacgc ctcatcatgc
     3001 ccggtcaccg agtttgcgca cggcccgctg ttcgatgtca ttggcaatgt ctggcagtgg
     3061 accgagaccc cgatctatcc cttcgatggt tttcaggtgc acgcggttta tgatgacttc
     3121 acgacgccga cgtatgatgg gcgacacaac atcatcaagg gcggatcgtg gatatcctgc
     3181 ggcaatgaag cgctgcctgc atcgcgctat gctttccgcc gccacttttt ccagcatgcc
     3241 ggattccgtt acgtgaagtc cggtgcgccg gcaacatcgc aaacatccca gtacgaaacg
     3301 gatcggctgg tttccgaata cgccgaattc cactacggag acgaatattt cgacgtgccg
     3361 aatttcccgc gcgcgctggc gcagttagcc atcgccgcga tgggcgaaaa accggccgac
     3421 aaggctctgg acctgggttg tgccacgggc cgcgcgacgt ttgaactggc gcggcatttt
     3481 gagcgggtga ccgggatcga tttttcggcc cgcttcattg gcctgggcac acggcttgcc
     3541 gaacagggca ggctacgtta caccctggtc gaggagggcg aactggtggc tttcaaggaa
     3601 cgcagtctcg ctgaattggg gctcggcccg gtcgcgcaga aagtcgagtt tttccagggt
     3661 gacgcctgca atctcaaacc ggtgtttacc gggtacgacc tgatcctcgc tgccaatctg
     3721 atcgatcgcc tttacgatcc ggagaaattc ctcgaatcgg tacacgagcg gctcaatccc
     3781 ggcggcgtgc tgttgctcgc ctcgccctac acctggctgc cggaacacac caagcgtgaa
     3841 gcatggatcg gcggattcaa aaaggacggc gagaatttca gcaccttcga cggtttgaaa
     3901 gccatcctcg gcaagcactt ccgcctgctg cgcggaccgt tctccgttcc attcgtcatt
     3961 cgcgaaacca aacggaagtt ccagcatacg ctgtccgaag tgagcatctg ggaacgcaac
     4021 tgatcacccg gcatttgcgg caaatacagg ctacgactcg tcgtcaatct gcgtgagcgc
     4081 ctgcgctata gcgctccgat cggtcggccg gatcaaacgc gcgacttcct gcccccggcg
     4141 catgaaaacc agtgtcggcc aaagtttcac gccaaatgaa cgccccaggc ggcgacccgg
     4201 accgtcttcg atcttgatac gccgcgttcg tggatgatca ccaagggccg acgcaatcag
     4261 cggctgcgcg gcaatgcagt agccgcacca agacgcgccg aactcgatga cggcgggttc
     4321 ctgcaaggcc tcgatctcgg cccgagtcgg ttcggctgca gaagagctga gatgacgttc
     4381 catcgattct ccgggcgcgc cattcgccta tttgaagacc gacagcattt ccatggtgtc
     4441 ggcctcgact tccggcagca ggtctttgaa gctctgccga acggaacctg catcgccggc
     4501 atccggatcg gtgtcttgtc gcgaccattc aaagtgagtg ccggcaatga tgttgccgac
     4561 gtagacgatg tccgacaacg tccgcaccgt tgtcgatatc gggcgtggtt gatcatgatc
     4621 gatggtggcg ttgacgattt cttcgggcat gcccaaggcg ttgagcagcg ttacgccgac
     4681 accttcgtgc cagcgcatga tgatgtactt taccgtatcc gggcgcagca acaactcagg
     4741 atattgaact gctcggtata gcatgtagaa ggcgcccagg tcatgcacta gcccagccag
     4801 cagcgcttca tcgggattga tccgggtgta ggtgcgcgcc aggatgcgtg cggccgccgc
     4861 agtccggatc gaatgatccc agagcgcgcc ggcaaggttg ccgaagatcg ccatgtcttt
     4921 ggaatgcagg agctgggcca tggcgatagc cagggcggtg gtgcgtacga catcgacacc
     4981 caggcgattg atcgccgcgc gcaaatcctt ggctttcgag ccgtcgcggc tgtaataagc
     5041 agaattggcc agttgcatca gcttggcagc agtcaggggt tcgatgccga cgatctttgc
     5101 aatgcgcgcg gttggcagat ccgggttctg caactccttg cgcaggcgca gagcggcgtc
     5161 gaaataggtg gggaaaacca catcgccagc cagttcgcgc gcgatatcct ccaacatgcg
     5221 gaagcgtgtt gcgttaagcg cctcgccgaa cttgtcgacc gtcggatccg gttctggctc
     5281 ctggatttgc tcctgatcct gttcttcgtt cgtcttcatg aattgcgagc tttagtcatg
     5341 ctgtcgtgac cgacaaggga gaattcaaaa tcacccctag agtatctgtg agtcatgcac
     5401 ccgacgtcaa atttgattgg acccaggctg gtgaattttc aacccagcct gattttctcg
     5461 ccatagcccg tcatttccag ataaccccgg ccaatttcac tgccgccctc ctcaactcgc
     5521 acggcccctt cccaataaat ggcgcttgtc gttcggcggc tgtccagttc ctggtcgtca
     5581 atcagcggta tcagatggag ctgtcgctcg cttattcgta cgcgccactc gaccgggtag
     5641 ctgattgcgg tacgcggcga tcgccagcgg cgcacggatg taaagccgat aacttcgggc
     5701 ggcagcgcct gtacgcggct tccgggctgc cggaatgtgg cggcggacca cagcggagta
     5761 ccgtcctttc cgcgcaagcg gaaagccatc agtgcgctac cgtcggtcag gttcaaacca
     5821 atccagtccc agccttgtgc ggacgcgtgc aggagttcgc tcgaccactc gtggtccagc
     5881 caagccaggc cggttacttc ttgtcgttgg ccgttgaggg ttacccagcc actcaccttg
     5941 agttgcggtc gactgtagta gtagctggcg tggcccgcct gcggcgactt ggcgctaaat
     6001 ccggcgtcgc cgttgagcat cggagggcca tcaggcatta gcgccagcgc gtaggaaaaa
     6061 tcctcgccgc gaacctccgc ctgatagccg ccttcgcgct gttcgagccg ccagtcgcca
     6121 atccatacgt ttgtccgccc cgtctcgaat ccgctgcgcc caaaaccgac acgtgcggcg
     6181 cgctgttcgt ggcgcaagcg gcccaggcgc gggtcggcca gggccgcgtg ggcgaggatc
     6241 agctgacggg gagcgaaagc gctcgggttg tcctctccga ttccggtacg gacgcgaaag
     6301 aaagtgctct gaaaaccgag cggagtaccg tccggcaagt tcagccaacc ggtggcatac
     6361 caccactcgg tgcggaaatc cgggtgcgca ccgtgatcga cgggaaacgt cagtttgcgg
     6421 ccgggcagta ctggcgcgaa ttcaaccgtc tccacatcgg cctgcgccac tccggcacgc
     6481 caagttgaca ggctggccag cgcgagcagg aaggcgcggc gatccatcac cagtcctcgc
     6541 gcaccgcaag aatcgcgccc tgacgcatgg cctgccgacc ggcggccacc gcggccagtg
     6601 ccgccaagac aatcaggacg ctagcgaaaa cggccagcga gccataggga atgcgcagat
     6661 ccatgctcca gtgaaagctc tgccgattga ccacctcgat cagcaccagc ccgatcgccg
     6721 cgccggccag cagtccgccg ataaccccga cgcctgccgc caaggcgcct tcctgggcca
     6781 gcatccagcc gatctggcgg cgcgtcaggc ccagatggcg gagcatcccg aactcgcgtc
     6841 gccgcgccac cgccagcgcg gcgaaactgg cggccacgcc agccagaccg atgatcacgg
     6901 cgacggcttc gagcaggtag gtgacggcga aagtgcggtc gaagatgcgc aggctgatgg
     6961 cgcgaatctc gcccggatag gcgatgctca gtgttcccgg gctgctcgtg gcagtgagcg
     7021 cggcggccac ctgatcgggc gcaacgccag gtgcaagcag gattgccgcg tcattgacca
     7081 actgatcgcc ggtcaggcga cggtagtccg ccaggtcgat catcagtgcg ccagtctgcc
     7141 gcgcatagtc acgccaaacg cccgccacgc gcatacgaac cggtcgcccg gccagcggca
     7201 gttccacctg ctggccgggc tgccagccat aaatatcctg cagtgcttcg gaaatccaga
     7261 ttgccggttc cttgctactg ccgtcgagaa ctgcggtgcc aaccagaggc agttcgcgcc
     7321 cgtcggccga caccggtcgg gcgatcaagg ccaccggcgt ctgccccgcg accaggcgca
     7381 agcggtcgtg acgggtaaaa ttcacgcgcc caacaccggt cacggcggct atccgggctt
     7441 gcag
//
LOCUS       DAORPM010000109         7437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2533_length_7437_cov_10.203603, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000109 DAORPM010000000
VERSION     DAORPM010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2533_length_7437_cov_10.203603"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLD53_06710"
     CDS             complement(<1..404)
                     /locus_tag="PLD53_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRC60639.1"
                     /translation="MSANPVIQESFADLFEASLARQEMRQGEVITAEVVRIDQNFVVV
                     NAGLKSESYIDREEFLSDQGELEVNVGDFVQVAIEQLENGFGETRLSRERAKRVAAWN
                     YLEAALNEGTLVTGTITGKVKGGLTVMANSVRA"
     gene            complement(577..2520)
                     /locus_tag="PLD53_06715"
     CDS             complement(577..2520)
                     /locus_tag="PLD53_06715"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HRC60640.1"
                     /translation="MDFVDLPPLLSAKGVVRLPGSKSISNRVLLLAALADGQTEVRDL
                     LESDDTARMIDALRLLGVVVESLGDRAYRVHGVAGKFPCRQAELFLGNAGTAFRSLTG
                     ALALAGGHYTLTGVARMHERPIGDLVDALRQLGADIRYLGNEKFPPLEIRPSAIRSGG
                     VLQVRGDLSSQFLTGLLMALPLTGVETTVEVVGDLISQPYIEITLATMARFGVQVERQ
                     GWQRFTLPAGHAYRSPGVVSVEGDASSASYFLALGAIGGGPLRVEGVGRDSVQGDVRF
                     ADALALMGARVERGPNWIETAGPLQGKLHGIDLDCKHIPDAAMTLATTALFAEGATTL
                     RNIASWRVKETDRIAAMATELRKLGAAVEEGADFIRVTPPHSSFLTPPAGIDTYDDHR
                     IAMCFSLAAFANTLRINDPGCVTKTFPDFFARFAAVTQPVPVIAIDGPSASGKGTVAA
                     RLASTLGWHYLDSGALYRLTALACRRAGVTWDDEAATATIAAGLDVVFGENSIRLSGD
                     EVNDAIRDEEISSGASQVAALPAVRDALLFRQRVFRRAPGLVADGRDMGSVVFPDALT
                     KVFLTASVEVRAERRHKQLIEKGIAASILPLLLDLRERDQRDSQRSVAPLQQSEDANL
                     LDTTDLTIEQAVSQVLSWSKQGA"
     gene            complement(2526..3410)
                     /locus_tag="PLD53_06720"
     CDS             complement(2526..3410)
                     /locus_tag="PLD53_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRC60641.1"
                     /translation="MSGSPEFGKLVIFGVGLIGGSFALGLKAAEQVEEVVGFGRSLST
                     LTQAMDLGIIDRVGANAGQEVADADLVLMATPVGQMPEIMARIAPYLGAQTVVTDGGS
                     TKADVVASAREHFSDKLGQFVPAHPIAGAENSGAAAARADLYRDKKVVLTPLPENPVL
                     NVARVRSAWEWCGAQVHELPPADHDRIFAAVSHLPHLLSFALVYELAVRENCDQFFDF
                     AASGFRDFTRIAASHPEMWRDICLANRPALLDELDRYRAQLDTLRDALQRDDGALLER
                     TFDVARKARRNWADGKGK"
     gene            complement(3407..4510)
                     /gene="hisC"
                     /locus_tag="PLD53_06725"
     CDS             complement(3407..4510)
                     /gene="hisC"
                     /locus_tag="PLD53_06725"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRC60642.1"
                     /translation="MALCDQSLPNVRAISPYLPGKPITQLAREMGIPVERIVKLASNE
                     NPLGMSPKAKVAVEKALVSLERYPDDFELKHALANHVALGMEHIVLGNGSNDVLDLIA
                     RVFLSPGRSAIFSQHAFAVYPLATLSAGAELIQVPAKDYGHDLEAMGNAIRIDTRLIW
                     IANPNNPTGTFLTYPQLKAFLRATPPDVVVVLDEAYNEYIPPLERADTTAWLAEFPNL
                     VVTRTFSKIYGLAGLRIGYALASVAIADLMNRVRQPFNCNNLALAAATAALDDHEFVA
                     RSYALNRSGMEQIVTGLKHFGFSHLPSHGNFVTFKAGDAAALNQRLLKQGVIVRPIGG
                     YGMPEWLRVTIGTETENFRFLEALDNALTRPQQ"
     gene            complement(4539..5630)
                     /gene="pheA"
                     /locus_tag="PLD53_06730"
     CDS             complement(4539..5630)
                     /gene="pheA"
                     /locus_tag="PLD53_06730"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRC60643.1"
                     /translation="MSDELQQKLSIVRAEIDAIDAQLIELLNRRAKCAQQVGEIKASH
                     GDAGYIYRPEREAQVLRRIQEINPGPLSNESVTWFFREVMSACLSLEQALGIAFLGPL
                     GTFSESAATKHFGHAARLMPQVSIDDVFREVEASHADYAVVPIENSTEGAIGRTLDLL
                     LTTPLKICGEVVLRIHQHLLSSETSLGNISKVYSHAQSLAQCHEWLNRSLPNAQRIPV
                     ASNAQAAQCAAAEAGTAAIAGEAAAERYQLPKLASSIEDEPNNTTRFVVLGRQEAGPS
                     GRDKTSLIMSAHNRTGALGKLLAPFSESGVSMTRLESRPARHTLWEYVFFVDIEGHRE
                     DPAVATALAELGQRAAYLKVLGSYPVTVY"
     gene            complement(5701..>7437)
                     /locus_tag="PLD53_06735"
     CDS             complement(5701..>7437)
                     /locus_tag="PLD53_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRC60644.1"
                     /translation="DGISDLRDESDKSGMRVVIELKRGEVPEVVLNCLYKQTQLQDTF
                     GMNMVALVDGQPRLLNLQQMLDCFLSHRREVITRRTVFELRKARERAHIQEGLAVALD
                     NVDEIIALIKASPTPADARRGLMARLWLSTTVGEMLQRAKLDATRPEGLGLEYGLSKS
                     GYLLSEAQAQAILELRLQRLTGLERDKIVADFADLLERIADLMDILARPERISEIIVE
                     ELTGIRAQFGDKRRSEIVLNTQDLGIEDFITPQDMVVTLSHTGYIKSQPLTDYRSQRR
                     GGRGKQAAAVKEDDFVDHLFVANTHDFILCFSNRGRVYWLKVYEVPQGARSGRGKPIV
                     NMFPLEEGEKINAILPIKEFTEDRFIFMCTSLGTVKKTPLSDFSNPRKAGIIAVALDD
                     DDFLIGAEITSGTHDIVLVSDAGKAVWFDEEDVRPMGRTARGVRGMKLGEGQQVLSLL
                     VAENHQETVLVATENGFGKRTVLADFRHSGRGTQGVIAIAASERNGKVVAARLVHDED
                     EIMLITTGGVLIRTRVSEIRELGRATQGVTLIALDAGEKLAGLEKVVETEDDQDVVPE
                     SGDEKAPGMDQS"
BASE COUNT         1643 a         2251 c         2051 g         1492 t
ORIGIN      
        1 gcacggacgc tgttggccat gacggtcagg ccacccttga ccttgccggt gatggttccg
       61 gtgaccaggg tgccttcgtt caaggcagcc tcaaggtagt tccatgcggc aacgcgtttg
      121 gcgcgttcac gcgacaggcg ggtctcgccg aaaccgtttt cgagttgctc aatggcgacc
      181 tgcacaaagt cgccgacatt gacttcaagt tcgccctgat cgctcaggaa ttcttcgcga
      241 tcaatatagc tttcggactt caatcctgca ttgacgacca caaaattctg gtcgatgcgc
      301 accacctcag cggtgatgac ttcaccctgg cgcatttcct gacgcgcaag gctggcttca
      361 aaaagatctg caaaactttc ctggataacg ggattagctg acatatataa aatgacctgt
      421 ccgcatggct gcgggtaaag ttaataaaaa atcaccgatt ctgatcgcta ccccagcgga
      481 acacgaagtg tcctgacggg catgcgaaat gtattgaacc ggcaacaatt aagggaacgg
      541 catttgccca cacccggcat ttacacactc ccttttctat gcgccctgtt tgctccagga
      601 caagacctga gacacggctt gttcaatggt caaatctgtc gtatcgagca aattggcgtc
      661 ttcactctgc tgcaagggcg caacgctacg ttggctatcg cgttggtcac gttcgcgcaa
      721 atccagcaaa agaggcagga tactagcagc gattcctttt tcgatcaact gcttatgacg
      781 tctttccgct cgaacttcaa cgctcgcagt gagaaaaacc ttggtcagcg catccggaaa
      841 taccactgat cccatatcgc gaccatcggc gaccaaaccg ggagcccgcc ggaagacgcg
      901 ctgtctgaag agcagcgcat cacgcactgc aggcagcgcc gcaacctggg aagcccctga
      961 ggagatttcc tcgtcgcgaa ttgcgtcatt gacttcatcg ccggacagcc gtatcgagtt
     1021 ctcgccaaac accacatcca gtccggcagc gatggttgct gtggccgcct cgtcgtccca
     1081 cgtcacgccg gcgcgtctgc aggccaaggc ggtgaggcga tacaaggccc ccgaatcaag
     1141 gtaatgccaa cccagtgtcg aagcaagacg tgccgcaacc gtacctttgc ccgaggctga
     1201 tggaccgtca atggcaatta ccggaacagg ctgcgtgacc gctgcgaaac gtgcaaaaaa
     1261 atcggggaaa gtcttggtga cacaacccgg atcattgatg cgcaaggtgt tagcaaacgc
     1321 tgccagggag aaacacatgg cgatgcggtg atcgtcatag gtatcgatac ccgcgggagg
     1381 agtgaggaac gaggaatggg gaggggtgac gcgaataaag tcggccccct cctcgaccgc
     1441 cgccccaagc tttcgcaatt ccgtcgccat ggcggcaatg cggtcggttt ccttgacacg
     1501 ccagctggcg atattgcgca gcgtcgtcgc gccttccgca aacaacgcgg tcgtcgccag
     1561 cgtcatcgcc gcatccggaa tgtgcttgca gtcaaggtca atcccgtgca actttccctg
     1621 cagtggcccg gcagtttcaa tccagttcgg gcctctttcg acgcgggcac ccatcagcgc
     1681 caacgcgtcg gcaaaacgca catcaccctg aaccgaatcg cgaccgacgc cctcaacgcg
     1741 tagcggaccg ccgccaatcg caccaagggc aaggaagtac gatgccgaag aggcatcacc
     1801 ttcaacggac accacccctg gcgaacgata ggcatggccg gcgggaaggg taaatcgctg
     1861 ccacccttgg cgctccacct gtacaccgaa gcgcgccatc gtcgccaggg tgatttcaat
     1921 gtagggctgg gagatcagat cgcccaccac ctctaccgtg gtttctacgc cggtcagcgg
     1981 caacgccatc aacaggccgg tcaggaactg gctcgacaga tcgcctcgta cctgcagtac
     2041 gccaccggaa cggatcgcgg acgggcgaat ctcgagcggc ggaaattttt cattcccaag
     2101 atagcggatg tcggcgccaa gctggcgcaa ggcgtcgaca agatcgccga tgggtcgctc
     2161 atgcatccgc gccacgccgg tcagcgtgta gtgcccacct gccagtgcca gcgccccggt
     2221 cagcgagcga aacgccgttc cggcgttgcc tagaaacagc tcggcctgcc ggcacggaaa
     2281 tttgccggcc acgccgtgca cgcgataggc gcgatcaccc agagactcca cgacaacgcc
     2341 aagcaatctc aatgcatcta tcatgcgcgc tgtatcgtcg gactccagaa gatcgcgaac
     2401 ttcagtctgt ccgtcggcga gcgcggcgag tagaagcacg cgattggata tgctctttga
     2461 gccgggcaga cgcactacgc cttttgcgga cagcagcggc ggcagatcga cgaagtccat
     2521 gaccatcact tgcccttgcc gtccgcccag ttgcgacgcg ccttgcgggc gacgtcgaag
     2581 gttcgctcaa gcagggcgcc gtcatcacgc tgcaacgcat cgcgcaatgt gtccagctgc
     2641 gcgcgatagc ggtcgagttc gtccagcaac gctggacggt tggccagaca gatatcgcgc
     2701 cacatttcgg gatggctggc agcaattcgc gtgaaatcgc ggaatccgct tgccgcaaaa
     2761 tcaaaaaact gatcacagtt ctcgcgcact gccagttcat ataccagagc aaaagacaat
     2821 aggtgcggaa gatggctaac ggcggcaaaa atgcgatcat gatcggccgg cggcaactcg
     2881 tgaacctgtg cgccacacca ttcccaggcc gaacgtacac gagccacatt aagaacgggg
     2941 ttttccggca agggagtcag caccactttc ttgtcgcgat aaagatcggc gcgcgccgcc
     3001 gctgccccac tgttctcggc tccggcaatg ggatgcgcag gaacaaattg tccaagcttg
     3061 tccgaaaaat gctcacgtgc tgacgcgacc acatccgctt tggtgctacc accgtcggta
     3121 acgaccgtct gagcgccaag atagggagca atgcgcgcca tgatctccgg catctgaccg
     3181 accggagtgg ccatcaaaac aagatcggca tcggcgactt cctgtccggc attggcaccc
     3241 acacgatcga taatacccag gtccatggcc tgagtcagcg tggacaggct acggccgaag
     3301 ccaacgactt cctcgacctg ttcggcagcc ttgagcccga gagcgaagga cccgccgatc
     3361 aggccaacgc cgaagatgac cagcttgcca aattcggggc ttccgctcat tgctgcggcc
     3421 tcgtcagcgc gttgtccaac gcttcgagaa atcgaaagtt ttccgtctcg gtgccaatgg
     3481 tcactcgcaa ccactctggc ataccgtagc cgccaatcgg acggacgatc acgccttgct
     3541 tgagcagcct ctgattcaga gccgccgcat cgccggcctt gaatgtcacg aaatttccat
     3601 gcgacggaag atgcgaaaag ccgaagtgct tcaggccggt gacgatctgc tccatgccgg
     3661 agcgattgag cgcatagctg cgtgccacaa actcgtgatc gtccagtgcg gccgtcgctg
     3721 cggccagcgc cagattgtta cagttgaagg gttgacgcac gcgattcatc agatcggcta
     3781 ttgcaaccga tgccagcgca taaccaatac gcaagccggc cagaccgtaa atctttgaga
     3841 aggtccgcgt gacgacaaga ttcggaaact cggccagcca ggcggtcgta tcggcacgtt
     3901 ccaatggagg aatgtactcg ttgtacgctt catcgagtac gacaacgaca tccggggggg
     3961 tagctcgcag aaaggccttc agttgcggat atgtcaggaa ggtgccggtc gggttgttcg
     4021 ggttggcgat ccagatcagc cgggtgtcta tgcgaatcgc attacccatg gcctcaaggt
     4081 cgtgtccgta gtcctttgcc ggaacttgga tcagttcggc acctgccgaa agtgtcgcca
     4141 acggatacac ggcaaaagca tgttgcgaaa aaatggccga acgacccggt gacaaaaata
     4201 ctcgggcaat caaatcaaga acgtcgttcg atccgttgcc tagaacgatg tgctccatgc
     4261 ccagagcaac atgattcgcc agcgcatgtt tcagctcgaa gtcatcaggg tagcgctcca
     4321 gtgaaacgag ggctttttca accgccacct tcgctttcgg gctcatgccc aagggattct
     4381 cgttggaagc cagtttgacg attcgctcaa ccggtatgcc catttcgcga gccagttgag
     4441 tgatcggctt gcccggcaga tagggtgaaa tggcacgaac gttaggcaac gactggtcac
     4501 acagcgccat ggatgatcct tgagaagttg gcgggtcgtc agtagacagt aaccgggtag
     4561 gaacccagaa ctttcagata ggctgcgcgc tggccaagct cggccagcgc cgtcgccacg
     4621 gcgggatctt ctcgatgccc ttcgatatcg acgaaaaaga cgtactccca caaagtatgc
     4681 cgcgccggcc tggattcgag ccgggtcatc gatacaccgg actcggaaaa tggcgcgagc
     4741 agtttaccga gcgcacctgt ccggttgtgc gccgacatga tcagcgatgt cttgtctcgc
     4801 cccgatggcc ccgcttcctg acgaccgaga acgacaaaac gtgtggtgtt gttcggctcg
     4861 tcttcgatgc tgctcgccag ttttggcaac tggtaccgct cggcggcggc ctccccggca
     4921 atcgccgcag ttcctgcctc ggcagcagcg cactgagccg cctgagcgtt gctggccacc
     4981 gggatacgct gggcattagg cagggaacga ttcagccatt cgtggcattg cgccagtgat
     5041 tgtgcatgcg aataaacctt gctaatgttg ccaagcgatg tttcactcga cagcaaatgc
     5101 tggtgaattc gcagcacgac ttcgccacag atcttcagcg gtgtggtcag caagagatca
     5161 agggtgcgcc cgatcgcgcc ttcggttgaa ttctcgatcg gcaccacggc ataatcggcg
     5221 tgcgacgctt caacctcacg aaacacgtca tcaatcgaaa cttgcggcat cagtcgcgcc
     5281 gcatggccga agtgtttggt tgccgcgctt tccgagaagg tgcccagcgg accgagaaag
     5341 gcaataccga gcgcttgttc aagcgacaga caggcggaca tgacctcgcg gaaaaaccag
     5401 gtaacgctct cgttcgacag gggccccgga ttgatttcct gaatgcgacg caacacttgc
     5461 gcctcgcgct cggggcgata aatgtagccc gcatccccgt gacttgcctt gatttcccca
     5521 acctgctgcg cacacttggc gcgacgattg agaagctcga tcaactgtgc atcgatggcg
     5581 tcgatctcgg cccgtactat cgagagtttt tgctgaagct catcgctcat ggtctatatc
     5641 cggcatggtg tcggcatttg cggctcattt gatcatgatc tcatgctcgg ggatcggact
     5701 ttacgattga tccatccctg gagctttctc atcgcctgac tccggaacga catcctgatc
     5761 atcttccgtt tcgacaactt tttccagacc ggcaagcttt tcgccggcat ccagcgcaat
     5821 tagtgtaacg ccttgcgtgg cacggcctaa ctcgcggatt tcgctgacgc gcgtgcggat
     5881 cagcacaccg cccgtggtaa tcaacatgat ctcgtcttcg tcatggacca atcgggccgc
     5941 gacgactttc ccattgcgtt cgctggcggc gatggcgatc acaccctgcg ttccgcgacc
     6001 ggaatggcgg aaatcggcca gaacggtacg ttttccaaaa ccattttcgg tcgccaccag
     6061 cacagtctcc tgatgatttt ccgcaaccag cagggagagc acctgctggc cttcgcccag
     6121 cttcatgccg cgcacaccac gcgccgtacg acccatcggc cgtacgtctt cctcgtcgaa
     6181 ccagacagcc ttgccggcgt ctgaaaccaa aacgatgtca tgggtgccgc tggtgatttc
     6241 ggcaccgatc aggaagtcgt catcgtcgag cgctacggca attattccgg ccttgcgcgg
     6301 atttgagaaa tcggaaagcg gtgtcttctt gaccgttccc agcgaggtac acatgaagat
     6361 gaaacgatct tccgtgaatt ccttgatcgg caggatggca ttgatcttct cgccttcttc
     6421 aagcgggaac atattgacaa tcggcttgcc gcgcccactc cgggcaccct gcggcacctc
     6481 atataccttg agccaataca ccctgccgcg attcgagaag cagagaatga aatcgtgcgt
     6541 attggcaacg aacagatgat cgacaaaatc gtcttccttc accgctgctg cctgcttccc
     6601 acgcccccca cgacgttgcg atcgatagtc ggtgagcggt tgtgacttga tgtaacccgt
     6661 atgcgaaagg gtgaccacca tatcctgcgg cgttatgaaa tcctcgatac ccagatcctg
     6721 cgtgttgaga acaatctccg agcgccgttt gtccccgaac tgggcgcgaa ttccggtcaa
     6781 ctcttcaacg atgatctcac tgatgcgctc cggtcgagcg aggatgtcca tcaggtcggc
     6841 gatgcgctca agcaggtcgg cgaaatcggc aacaatcttg tcgcgttcaa gtccagtcag
     6901 acgctgcagt cgcagttcga ggatagcttg cgcctgtgct tcggaaagaa gatagccact
     6961 tttcgacaaa ccgtattcaa gccctaatcc ttccggacgc gtggcatcga gctttgcacg
     7021 ctgcagcatt tcacctaccg tcgtcgacag ccacaaacgc gccatcaagc cgcgccgcgc
     7081 atccgcgggg gtcggtgacg ctttgatcag cgcaatgatc tcatcgacat tgtcgagcgc
     7141 tactgccaag ccttcctgaa tatgagcccg ctcgcgcgcc ttgcgcaact cgaataccgt
     7201 gcgccgggta atgacctcac ggcgatgcga aaggaaacag tccagcatct gctgcagatt
     7261 gagcaggcgc ggctgaccat cgaccaacgc caccatgttc atgccaaagg tatcctgcag
     7321 ctgcgtctgt ttgtacaggc agttaaggac gacttcgggc acttccccgc gcttgagctc
     7381 gatcaccacg cgcatacccg acttgtcgga ttcgtcacgc agatcggata tgccgtc
//
LOCUS       DAORPM010000110         7390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2550_length_7390_cov_9.148875, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000110 DAORPM010000000
VERSION     DAORPM010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_7390_cov_9.148875"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLD53_06740"
     CDS             complement(<1..261)
                     /locus_tag="PLD53_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60645.1"
                     /translation="MTPFFGVPGWAIHLARRVLYFWVRTTVLPERPEKLGLDPSKPVC
                     YVLQHPHLSNLLVLFRESRLAGLPSAEAPFRLGSDDFPRSVFF"
     gene            complement(292..2349)
                     /gene="fusA"
                     /locus_tag="PLD53_06745"
     CDS             complement(292..2349)
                     /gene="fusA"
                     /locus_tag="PLD53_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRC60646.1"
                     /translation="MGHNTTVNIRTVALVGHGATGKTTLAETLLATAGAISSHGSVER
                     GNTVCDFDAQEKELGHSLNSAMFTLDWLNTQVHMIDTPGYPDFAGQAIGALAAVDTAL
                     IVINAQTGIELSTERMMRLAAGRNLCRMIVINKIDADNVDLPRLVGEIRERFGRECML
                     LDLPAHHAQDVVELLGHDSGDSDFESVANAHRALIDQIVEEDEDLLARYLDEGTDPSI
                     DELHAPFEKALREGHLIPILFVSARTGAGIPQLLDVLARLAPNPTEGNPPPFYKGEPG
                     DPDSVPFHAEPDANQHVLAHVFKVVSDPFIGKLGVFRVHQGTIRKDSQLFVGDTKRPF
                     KVGHLYRLQGKENVEVEALVPGDIGAIAKIDEIEFDCVLHDSHDEDHIHLKPLEFPQP
                     MQGLAVETQRKADEQRLFDVLHKLEIEDPCFKVERHPTTNETVIRGLGDMHLRAKLAR
                     MAQQYKIELNTRPPQIAYRETITAKAEGHCRHKKQSGGAGQFGEVMLRVEPLERGAGF
                     EFVDIVKGGTIPGVFMAAVEKGVRQALEDGVVGGFPVHDLRVIVHDGKSHSVDSKDIA
                     FFSAGRKATIDAIRSSSPIILEPVVDIEVLAPEAAIGDLTGDLSSKRGQVTGTQPRGV
                     GTISISGKVPLAELDDYQGRLKSLTGGQGSYSISFSHYAPVPAGTQQQLASQRKSVGL
                     EDD"
     gene            complement(2512..5217)
                     /locus_tag="PLD53_06750"
     CDS             complement(2512..5217)
                     /locus_tag="PLD53_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRC60647.1"
                     /translation="MSGQFQTSNNAMHIAHLSIPAALNSLRTSVDGLSPIEAEKRLRE
                     YGHNRIEEVKSDPQWRRLLREFTHFFAVILWVAAALAVFAETRSPGEGMWQLGLAILA
                     VILINGAFSYWQEYRAERAISALRNLLPSQVKVIRDGELLTLAAELLVPGDIVLLEEG
                     DNVPADCRLIEASGIRVNTATITGESLPKARTADGEAAIGAAASDASNLLLAGTALVS
                     GQGRAIVFATGMRTEFGKIAHLTQTAGKSVSHLQLEIARLSKLVAVFATGLGLVFFLV
                     GTLIGLPFWTNLMFAIGIIVANVPEGLLPTVTLSLAMATQRMAKRNALIRHLPAVETL
                     GSTTVICSDKTGTLTQNRMTVQQVFFENTLCNANEHHLGDAAASLLRNAQYCHNLKQS
                     LRDGKASWLGDPMEVALLSFARKVQDFGEAPLLSEIPFDSQRRRMSVVVEHEGQHWLY
                     CKGAPEAVLPLCTRTGQDGSTALDETSRQRIGEAQERMADQGLRVLAFAYRPLQEQES
                     PAERELIFSGLIGLHDPPRPEVPEAIARCQSAGIKVIMVTGDHPHTALAIAREIGLVT
                     GDRPLIVAGESLRKMTTAQLQIALDNPEALFTRVTAEQKMLVVQALKNKGEIVAVTGD
                     GVNDAPALKTAHIGIAMGVCGTDVAKEAADMILLDDNFASIVNAIEEGRAVFENIRKF
                     LTYILTSNIPEIVPYLAFVLFKIPLPLTVIQILAVDLGTDMLPALALGAERPDPALMR
                     EPPRPRDQRLLSWSLVARSYLWLGALQAGIAMSAYFFVLYLGGWHYGQTLGKTDPQYL
                     QATTACLAAIVIAQMVNVFMCRHPRLSALSFSLRQNHLLLLGLGVELSLILLIVYTPL
                     GNRIFGTLAFPPAVWPVIFLLALGFWVLEELRKFVLRVCAESPRQ"
     gene            5538..5837
                     /locus_tag="PLD53_06755"
     CDS             5538..5837
                     /locus_tag="PLD53_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60648.1"
                     /translation="MRKSLIASAALVAVFSAASVSADEQKFELKPSAGMNEVLTENVG
                     KRVTLRLGSGDEIEGTVTQVGKTLVHVSRISGKEYYDAVISIDKVSAVRMRVREK"
     assembly_gap    5868..5967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6164..7012
                     /locus_tag="PLD53_06760"
     CDS             6164..7012
                     /locus_tag="PLD53_06760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRC60649.1"
                     /translation="MNRIRRLCFLFVCSALGIMAPAHGAGADTAQAVLPVFDAHLHYN
                     WEPAPHMPLDTVLSLFREHKVTGILATSRPNDGTRALYEARPKDIWVVPFIRPYRVRP
                     DIQTWMNDPQTEELIATEFKRGYYRGIGEFHLTGSAAEASQVKNTVEFAVKHNLYLHA
                     HADDEALEILYRHSPKARIIWAHTGFSTEPAKVEAYLQRYPGLWCELSYRYGITEGGK
                     LTTEWRRLFEKYPERFLIGSDTWVNERWDNYGNIMATYRGWLAQLPAEVAEKIAFRNG
                     ERLFGR"
BASE COUNT         1506 a         2121 c         2167 g         1496 t
ORIGIN      
        1 aaagaagacg gaacgcggga agtcgtcgct gcccagcctg aagggcgcct cggccgatgg
       61 caggccggca agtcgtgatt cccggaagag aaccagcagg tttgataaat ggggatgctg
      121 caatacatag caaaccggct ttgacggatc aaggccaagc ttctccgggc gctccgggag
      181 cacggtggtc cgtacccaga aatacagtac ccggcgggcc agatgaatgg cccagccggg
      241 gacgccgaaa aagggcgtca tgacgcctag cctggcgaat tcacgatccg ctcaatcatc
      301 ctcaaggcca accgacttgc gttgcgacgc caactgctgc tgggttcctg cgggcaccgg
      361 agcgtagtgc gagaagctga tgctgtagga cccttgtcca cccgtcagcg acttgaggcg
      421 tccctggtag tcatcgagtt ctgcgagcgg aaccttgccg ctgatcgata tggtgcccac
      481 gccacgcggc tgtgttccgg taacctgccc gcgcttgctt gaaagatcgc cggtcaggtc
      541 gccgatggct gcctccggag ccagcacttc gatgtcaacc acgggttcaa gaatgattgg
      601 cgaagaggag cggatcgcgt caatggtggc tttccggccg gcagaaaaaa aggcgatatc
      661 cttgctgtcg acagagtgcg acttgccgtc atgcacgatg acgcgcagat cgtgtaccgg
      721 gaagccgccg acgacgccgt cttccaaagc ttgcctcaca cccttttcaa ccgcagccat
      781 gaatacgccg gggatggttc ctcccttgac gatatcgacg aactcgaagc cggccccgcg
      841 ttccaggggc tcgacgcgca acatgacctc accgaactgg ccggctccgc cgctttgctt
      901 cttgtgacgg cagtggcctt cggcttttgc cgtaatcgtt tcgcggtagg cgatctgtgg
      961 cggccgggtg ttgagttcga tcttgtactg ctgcgccatg cgcgccaatt tggcgcgcaa
     1021 gtgcatgtct ccgaggccgc gaatgacggt ctcattggtc gtcgggtggc gttcgacctt
     1081 gaagcagggg tcttcaattt cgagcttgtg cagtacgtcg aacagacgtt gttcatccgc
     1141 tttgcgttgc gtttcgacgg ccagcccctg catcggctgc gggaactcca ggggcttcag
     1201 atgaatatga tcttcatcat gagaatcatg aagcacgcag tcgaactcga tctcgtcgat
     1261 cttggcgatg gcgccgatgt cgccggggac cagcgcctcg acttcaacat tctccttgcc
     1321 ctgcagacga tacaggtggc cgaccttgaa cggccttttc gtgtcgccga caaatagctg
     1381 cgagtccttc ctgatcgtgc cctgatgcac gcggaagacg ccaagcttgc cgatgaaagg
     1441 atcgctgacg accttgaaca catgcgccag tacgtgctgg ttcgcatcgg gttcggcgtg
     1501 aaacggaacg ctgtccgggt cacccggctc gcctttgtag aacgggggag gattgccttc
     1561 ggtcggattg ggggccagcc tggccagcac atcgagcaat tgcggaatac cggcaccggt
     1621 tcgcgctgaa acgaaaagaa tcggtatcag gtgcccttcg cgcagcgctt tctcgaacgg
     1681 ggcgtgcaat tcgtcaatgc tcggatcggt tccttcgtcg agatagcgcg caagaaggtc
     1741 ttcatcttcc tcgacgatct ggtcgatcag cgcgcggtgg gcgttggcga ccgactcaaa
     1801 atcgctgtcg ccgctgtcgt gaccgagtaa ttcgacgaca tcctgggcgt gatgcgccgg
     1861 taaatcgagc agcatgcatt cgcggccgaa acgttcgcgg atttcgccca ccagcctggg
     1921 cagatcgacg ttgtccgcgt cgatcttgtt gatgacgatc attcggcaga ggttacggcc
     1981 tgcggccaga cgcatcatgc gctcggtcga tagctcgatt ccggtctgcg cgttgatcac
     2041 gatcaatgcc gtgtcaacgg cggctagagc gccaatggcc tgcccggcaa agtccgggta
     2101 acccggcgta tcgatcatgt gcacctgggt gttcaaccag tcgagggtga acattgcgga
     2161 attcagcgag tgtccgagtt ccttttcctg agcatcaaaa tcgcaaacgg tattgccccg
     2221 ttcaaccgat ccgtggctgg agattgcgcc ggccgtggcg agcaaagtct cggccagtgt
     2281 cgtcttgccg gttgcgccat gaccgacgag agcaacggtg cgaatgttga cggtggtgtt
     2341 gtgccccatg ttatctcctg aagatgattt gcaaatagtt gaggcgaggg taggcccacg
     2401 ctcatcgcgg cacagatgct ccctcgattt cttgtcagaa cccgcccatt ctacctcgtg
     2461 tggctgtatt ccaaagcagc tgcagcggca tgtataggct cttgtcgtga gttactgcct
     2521 tgggctctct gcgcatacgc gaagtacgaa tttccgcagc tcttcgagca cccagaagcc
     2581 cagcgcgagc aggaatatga caggccatac ggccggagga aaggcgagag tgccaaaaat
     2641 ccggttaccc agtggcgtat agacaatcag cagaatcagc gacagttcaa cgccaagtcc
     2701 gagcagcaac agatgattct gccgcagtga gaaacttagc gccgacagcc tggggtgtcg
     2761 acacatgaag acattgacca tttgggcgat gacgatggcc gccaggcagg ccgtggtggc
     2821 ctgcaagtac tgcgggtctg ttttccccag agtttgaccg taatgccaac cgcccagata
     2881 cagcacgaaa aaataggcgg acatcgcgat gcccgcttgc agcgccccca gccacaagta
     2941 cgaccgggcg accagcgacc aggaaagcaa gcgttgatct cgtggccgcg gcggttccct
     3001 catcagcgcg gggtcagggc gttcggcgcc cagcgcaaga gccggtagca tgtctgtgcc
     3061 aagatcaact gccagaatct ggatgacggt cagcggcagc ggaatcttga agagcacaaa
     3121 ggcgaggtaa ggaacgatct ccgggatgtt cgaggtcagg atgtaggtca gaaacttgcg
     3181 aatgttctcg aaaacggcgc ggccctcctc gatggcattg acaatgctcg caaagttgtc
     3241 gtcgagcagg atcatgtcgg cggcttcttt cgcgacatcg gttccgcaga cgcccattgc
     3301 aatgccgata tgtgccgtct tcaatgccgg agcgtcattc acgccgtcac cggtgacggc
     3361 cacgatttct cccttgttct tcagcgcctg aacgaccagc atcttttgtt ccgcggtgac
     3421 ccgggtgaac agggcttcgg ggttgtccag cgcaatctgc aactgtgccg tcgtcatctt
     3481 gcgcaatgat tcgcccgcga cgatcagcgg ccggtcgccg gtgaccagac cgatttcgcg
     3541 cgcaatggcc agcgccgtat gcggatggtc tccggtcacc atgatcacct tgatgccggc
     3601 ggactgacaa cgcgctatgg cttcgggcac ctcggggcgg ggcggatcgt gcagcccgat
     3661 cagcccggag aagatcaatt cccgttcagc cggactttcc tgttcctgca acgggcgata
     3721 cgcgaaggcc agcacgcgca ggccctggtc ggccattctt tcctgcgctt caccaatgcg
     3781 ctggcgggag gtctcgtcaa gcgcggtact gccgtcctgt ccggttcgcg tacacagcgg
     3841 caaaactgct tccggcgcac ccttgcagta cagccagtgt tgcccctcgt gctcaaccac
     3901 gaccgacata cggcgccgct ggctgtcaaa cgggatttcg ctcaacagtg gcgcttcgcc
     3961 gaaatcctgg accttcctgg cgaaagacag cagggcgact tccatggggt cgcccagcca
     4021 gcttgccttt ccgtcccgaa ggctctgttt caggttgtga cagtactggg cattgcgcaa
     4081 caatgacgcg gcggcgtcgc cgagatggtg ttcgttcgca ttgcacaatg tgttctcaaa
     4141 gaagacttgc tgcaccgtca tgcggttctg ggtcagcgtc ccggtcttgt cgctgcagat
     4201 taccgtcgtt gaaccgaggg tttcaacggc cggcaggtgc cggatcaggg cattcctttt
     4261 cgccatgcgc tgcgtggcca tggccagcga caaggttacc gtcggcaata gtccttccgg
     4321 gacattcgcg acgatgatgc cgatggcgaa catcagattt gtccagaacg gcaggccgat
     4381 cagcgttccg acgagaaaga ataccagccc aagccccgtg gcaaacacag cgaccagttt
     4441 tgaaaggcgg gcgatttcaa gttgcaggtg cgagaccgat ttgccggcgg tctgcgtcag
     4501 gtgggcaatc ttgccgaatt cggttcgcat gccggtggca aagacgatgg cccggccctg
     4561 accggaaacc agcgcagtgc cggcgagtag cagattgctg gcatcagatg ccgccgcgcc
     4621 gatagccgcc tcgccatcgg cggtgcgtgc cttgggcagc gattcgccgg taatggtggc
     4681 ggtattcacc cgtattccgg acgcctcgat caatcgacag tcggccggta cgttgtcgcc
     4741 ctcttcaagc agcacgatgt cgccggggac aagcaattcg gcagccagcg tcagcagctc
     4801 gccatcccgt atgactttga cctgactcgg caaaaggttg cgcaaggctg agatggcccg
     4861 ttcggcacga tattcctgcc agtacgagaa cgcgccattg atcaggatca ccgcaagaat
     4921 cgccaggccg agctgccaca taccctcgcc cgggctgcgc gtttcggcaa aaaccgctag
     4981 cgcggccgcg acccacagaa tcactgcaaa gaagtgggtg aactcccgga gcaggcgccg
     5041 ccattgcgga tcgctcttga cttcctcgat ccggttgtgg ccgtattccc gcaaacgttt
     5101 ctccgcctcg atcgggctca agccgtcaac ggacgtgcga aggctgttca gggcagccgg
     5161 aatgctgagg tgtgcgatgt gcatggcgtt gttggatgtc tggaattgtc cggacaagac
     5221 acggcggatt tcagagcctc attatccgat tcgccacacg atatgcaaat ataatcgact
     5281 ggtgcggcga ataggagtac aaccccgcaa atgaagtggc tttttccggc attttgctct
     5341 ccttactgac ccatcgtccg aacggcgatg ccgagtgcgg acacaagggc aagttcggag
     5401 ataccacttc actgcagtgt ggcgtagatt gatttgatcg tcagtaaaat agcctcagcg
     5461 ttgcccgagc caacgcgggc atctttgcgg cgattcattg gattgatatt ttggttcatc
     5521 cacgggaggt aagtgaaatg aggaaaagcc ttatcgcatc tgcagcgttg gttgctgttt
     5581 tttccgcagc atccgtctcg gccgacgagc aaaaattcga gttgaaaccg tccgccggga
     5641 tgaatgaagt cctgaccgag aacgtgggca agcgagtaac tctccgtctg ggttctggcg
     5701 acgaaatcga agggactgtc actcaggtcg gcaagaccct ggtgcatgtt tccagaatat
     5761 cggggaaaga gtattacgac gctgtgatca gcatcgacaa ggtcagcgct gtcaggatgc
     5821 gagtgcgcga gaaatagcgt cgcttgcatc agccactatg tttccagnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnncag gatgcgagtg cgcgagaaat agcgtcgctt
     6001 gcatcagcca ctatgtttcc agtggtacgg cgttacggga agctgccgcg ttcatgctta
     6061 gtggggcgca cactggtact gccgtctcac caacgccgac tctctaccct gcgcttgacc
     6121 gccaatcaac caagagcctt gccaataccg gctggagatc atcatgaacc ggattcgtcg
     6181 tttgtgtttc ttgttcgttt gttcggcatt gggaatcatg gccccggcgc acggcgccgg
     6241 cgccgacact gcgcaggccg tgctccccgt ttttgacgca catctgcact acaactggga
     6301 accggcgccg catatgccgc tggacacggt gctgtccctg ttccgagaac acaaggtcac
     6361 cggtatcctc gccaccagcc gacccaatga tggcacgcgc gcgctctacg aggcacggcc
     6421 gaaagatatc tgggtggtac ccttcatccg tccttaccgt gtgcgccccg acattcagac
     6481 atggatgaac gatccgcaga ccgaagaact gattgccacc gagttcaaac gcggctatta
     6541 ccggggcatc ggcgagtttc acctgacagg cagcgccgcc gaagcgtcac aggtcaagaa
     6601 caccgtcgag ttcgcggtga agcacaatct ctatctgcat gctcacgccg acgacgaagc
     6661 gctggagata ctctaccggc acagtccgaa ggcacgaatc atctgggcgc acaccggttt
     6721 ttccaccgag ccggcgaagg tcgaagccta tctgcagcgc tatcccggac tgtggtgcga
     6781 actgtcctac cgctatggca tcacggaagg cggcaaactc acgaccgaat ggcggcgcct
     6841 gtttgaaaag tacccggagc gctttctgat cggctccgac acatgggtca acgaacgctg
     6901 ggacaactac ggcaacatca tggctacgta caggggatgg ctggcgcagc tgcccgccga
     6961 ggtggcggag aagatcgcct tccgcaacgg cgaacgactc ttcggacggt aggcggtttc
     7021 tctccgaaca tgaggatatt gatgctgcct tcgacgaaaa acacccctcc cgattgcggt
     7081 ccctgtaagt gcgttatccg aattgacctc tttcatgcct caaagcaacc tcgctacttt
     7141 cgctcggagt cgggagtgtc agccggcaag aacagaatgt ggccactggg caaaatcgtg
     7201 gtgggaatag gaactatcgg ccacgaagag acattgcccc tgtgaagtat cgaaggccgc
     7261 gaacggcagg aacttgatca agttcctgcc aatcgatctt gctgggggca agtccattca
     7321 tcggcaagac caggcgggtt cgtcgggtac agcgaattca tttacccctt ttttacctgg
     7381 cgtgtagaac
//
LOCUS       DAORPM010000111         7350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2572_length_7350_cov_12.342838, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000111 DAORPM010000000
VERSION     DAORPM010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2572_length_7350_cov_12.342838"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLD53_06765"
     CDS             <1..299
                     /locus_tag="PLD53_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC60650.1"
                     /translation="QLRLRPILMTSFAFILGCVPLAIASGAGALSRQVMGGAVIGGML
                     AATGIAIFIIPVTYYFVEKMSKKGNSGVAQPAGGETGVGLATEKTAIKGGDTHA"
     gene            292..1737
                     /locus_tag="PLD53_06770"
     CDS             292..1737
                     /locus_tag="PLD53_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRC60651.1"
                     /translation="MRKSLTAPLLALLVSGCMIGPDYVRPPADAPAAWRLSDVQAEQL
                     ANADWWEQFNDPVLNDLVTTALRENKDIMIASARIEEYAGRYGVTRSQLFPQVGAGYN
                     AARQKNVLPGATNATTYNTYEALLGASWEIDLWGKIRRQSEAARAQIVATEEAKQGVI
                     LSLVASVAGGYINLRDLDRQLEIARDTARSRGESYKIFQDRYDGGVISILELSQNKSQ
                     YEEALASIPPIEFAIAQQENALSILLGRNPGPITRGRTIDELVPPVIPEGLPSDLLER
                     RPDILAAEQELIAANAEIGVAKAAYFPSISLTGVLGFASGSLSNLFNSGSKVWQYAGA
                     VGMPIFTAGGLAGQLLVSEAQQQQALLRYQQAIQNAFREVDDALVAQDRARVQLAAQR
                     QQVASLEQYAGTARLRYDNGYTSYLEVVDSERSLFNAQLAYTKTQQTQLQSMVILYKA
                     MGGGWMTKPEVAAEAKPEAVSEAESGVEEKK"
     gene            complement(1759..3189)
                     /locus_tag="PLD53_06775"
     CDS             complement(1759..3189)
                     /locus_tag="PLD53_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRC60652.1"
                     /translation="MTELISQIEALIVRHWEVLYSLLIVVGLAIYAAASHSLHQRRHP
                     AAAIAWVLGIVLLPYVALPLYLAFGSRKIVAKRAARGVRRVAAVAPTPPATAARAQQL
                     GMAMGLPDPVAYEGLSIHEDGTQALNALRCMIDGAKRTLEISTFVFGRDVLGDEIAER
                     LKQAARAGIKVRLLIDGIGIYLGGYPDLKGFAQAGVDVVRFVPPFRSPKRGRTNLRNH
                     RKMVIADGMHLWCGGRNLAAEYFEGDPRPILGRKPWVDLSFDLHGELARQAQSQFEQD
                     WAFATERPAPEVAVSATPSPVSGAMAQLVASGPDQPDDTLFTLLVSACFTSQWRILAV
                     TPYFVPDNSLLTALSLAARRGVDVNLVLPARSNHRLADFVRHRQLRDLVAAGGKVWLF
                     PRMIHAKLVLIDEELALAGSANLDGRSLFLNYEMMVAFYDHKAVHDFSQWIEVRRLES
                     VPYVAHRPGVVRELAEGMLLWLGFQL"
     gene            complement(3182..4762)
                     /locus_tag="PLD53_06780"
     CDS             complement(3182..4762)
                     /locus_tag="PLD53_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60653.1"
                     /translation="MTSPAYGLFPAADFMVTMGYRADSATIPQARWFFREETIAVPAH
                     GRPIEGLDPRLSATEALLGWNALTLPGSARDYPDLVWIGSPELIEQAWIDAKGENLRF
                     AGGLTGFSLTPRLESNRSYYNADSTDFFAQRELRLRGKTEGGRFVARSIWPADFRLDP
                     DAAFKPIAATPEAIRAFVRSEPRGGAQSNFTTQVIWQRDADSRRHRAGSPLLGIMLNG
                     AQGDDDEAHGGHFGLFTGRVGSQGQMHDWLMANFYTLETESEKGIIAAMLPLECYLAD
                     LNGGQAWYRPSCLLVATLRDERTALHLSSALARVFNQFYRHQFAYHHAAANCTGICIS
                     TLRTIGWRVPSLGPSSWLKAIAGLPVVAATTGSLSKGKAIFDYLTEDQTRLFPAIAFE
                     QAGADLLRLLKGSATRELTPYEELLRQDVEEIMLVRVPQLPSDRAWGDCPVASVDEYR
                     QRLPKDPAKQQIVPVDPRHFPPGLKDPSAPDKKPLRSDYAVAAYAALAVLIGGFLLRF
                     MSHRRRGKRERVLEQRDD"
     gene            4944..5561
                     /locus_tag="PLD53_06785"
     CDS             4944..5561
                     /locus_tag="PLD53_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRC60654.1"
                     /translation="MYHGEKFNAWTHFVGAVLSIAGAVVLISRASATGDPWKIVSASV
                     FGASLILLYSTSTLYHSLRGRAKIILRKLDHLTIYLLIAGTYTPFCLVTLRGPWGWSL
                     LGVVWGLAVVGMLQEIKPRSEARILSLVIYAVMGWVVLVAIKPLLEQLETAGFVWLAS
                     GGLLYTLGIVFYANDERFKHWHGIWHLFVIGGSLAQYIAVAGYVI"
     gene            complement(5584..6060)
                     /locus_tag="PLD53_06790"
     CDS             complement(5584..6060)
                     /locus_tag="PLD53_06790"
                     /inference="COORDINATES: protein motif:HMM:NF018946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60655.1"
                     /translation="MAQQYDKLASKFNELYLAGKARGRESMTEALDIAHKQMTALGEF
                     SAEQGEELKKYLSRDLDQTISDAQQLGEEAKERFNPSRLGAGALSSIANVMEFTGNAL
                     RSLSEKTKETLTYKTGEMTSAGTLTCKACGQSMQLKHTGHVPPCSKCSGTLFSKGY"
     gene            6422..7264
                     /locus_tag="PLD53_06795"
     CDS             6422..7264
                     /locus_tag="PLD53_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRC60656.1"
                     /translation="MRFEGSEQYVATRDLTLAVNAAITLQRPLLIKGEPGTGKTMLAE
                     EVAGALGLPLFQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDARVADIANYIVKGVLWE
                     AFNCDTPSVVLIDEIDKADIEFPNDLLRELDRMEFFVYETRQLVKARHRPILFITSNN
                     EKELPDAFLRRCFFHYIKFPDKETMVKIVDVHFPGLKKNLLREALEVFFELREIPGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRSQDQKVAIPPLHGALLKNEQDVHLFERLIFMA
                     RQNR"
BASE COUNT         1508 a         2216 c         2185 g         1441 t
ORIGIN      
        1 cgcaactgcg cctgcggccg atcctgatga cctcgttcgc cttcatcctc ggctgcgtgc
       61 cgctggccat cgccagcggc gccggcgcgc tttcgcggca ggtcatgggg ggcgcagtga
      121 tcggcggcat gctggcggcc accggcatcg ccatcttcat cattccggtg acctattact
      181 ttgtcgaaaa aatgagtaaa aaggggaata gcggtgtggc gcagcccgcg ggcggagaaa
      241 ccggggtcgg cctagcgact gaaaaaactg ccatcaaggg aggggatacc catgcgtaag
      301 tctttgaccg ctcccctgct ggcgctgcta gtcagcggtt gcatgatcgg gccggattat
      361 gtgcgcccgc cggctgacgc cccggcggct tggcgcctga gcgatgttca ggccgaacaa
      421 ctggccaatg ccgactggtg ggaacagttc aacgatccgg tactcaacga tctggtgacc
      481 accgctttgc gcgaaaacaa ggacatcatg atcgccagcg cgcgtattga ggaatacgca
      541 ggccgctatg gtgtgacgcg ttcccagctc ttcccgcagg tgggtgcggg ctacaacgca
      601 gcacggcaga agaacgtgct gcccggcgcg acgaacgcca caacctacaa cacctacgaa
      661 gctctgctcg gcgccagttg ggaaattgac ctgtggggca agatccgtcg ccagagcgaa
      721 gcggcacgtg cgcagatcgt agctacggaa gaggccaaac agggcgtcat tctgtcgctg
      781 gttgccagcg tcgccggcgg gtatatcaac ctgcgcgacc ttgatcgcca gcttgagatc
      841 gcccgggata cggccagatc acgcggagag tcctacaaga tttttcagga tcgttacgac
      901 ggcggcgtca tctcgatcct cgaactgagc cagaacaaat cccaatatga ggaagcgctg
      961 gccagcattc cgccgattga attcgccatt gcccagcagg aaaacgcgct cagcatcctg
     1021 ctcggccgga atcccggccc cataactcgg gggcgaacaa ttgatgagct ggttccgccg
     1081 gtcatcccgg aaggcttgcc ttccgacctg ctcgaacgcc gccccgacat tctcgcagcc
     1141 gagcaggagc tgattgccgc caatgcggaa atcggcgtgg ccaaggccgc ctacttcccc
     1201 tcgatctcgc tcacgggtgt cttgggtttc gccagcggca gtctttccaa tctatttaac
     1261 agcgggtcga aggtctggca gtacgcggga gccgtcggta tgccgatctt cacggcgggc
     1321 ggtctcgcgg ggcagctcct ggtttccgaa gcgcaacagc aacaggctct gctccgctac
     1381 caacaggcca tccagaacgc cttccgcgag gttgatgatg cgctggtcgc tcaagacaga
     1441 gcacgagtcc agctagccgc gcaacggcag caggtcgcct cgcttgagca atatgcaggc
     1501 actgcccgtc tgcgctacga caatggctac accagctacc tggaggtcgt tgactccgaa
     1561 cgcagcctgt tcaatgcgca actggcctac accaagacgc agcaaaccca gttgcagtcg
     1621 atggtcatcc tttacaaagc catgggtggc ggctggatga cgaaaccaga ggtggcagca
     1681 gaagccaaac ccgaagccgt atccgaggca gagtccggcg tcgaagagaa gaaataggcc
     1741 ttcgcccgtc gcttgcggct agagctggaa gcccaaccac aggagcatgc cttcggccag
     1801 ctcacgcacg acacccggac gatgcgcaac atagggaacg gattccagac gtcgcacttc
     1861 gatccactgc gaaaagtcgt gaacggcctt gtgatcgtaa aaggcaacca tcatctcgta
     1921 attgaggaaa agacttcgcc cgtcaagatt ggccgatccg gcaagggcca gttcttcgtc
     1981 aatcaatacg agcttggcgt gaatcatgcg cgggaacagc cagaccttgc ccccggcggc
     2041 gaccagatcc cgcaactggc gatgccgcac aaaatcggcc aaccggtgat tggacctggc
     2101 cggcaaaacc agattcacgt ctacgccgcg tcgtgctgct agcgacaggg ccgtgagcaa
     2161 cgaattgtcc ggcacgaagt acggggtcac tgccaggatt cgccactgcg aggtaaagca
     2221 ggccgagacc agcagggtga acagcgtatc gtccggctgg tccggaccgc tcgccaccaa
     2281 ctgggccatc gctccggaaa cgggtgacgg tgtcgcagaa acagccacct ccggcgccgg
     2341 gcgttctgtc gcaaaagccc aatcctgttc gaactgggac tgggcttgtc tggcgagctc
     2401 tccgtgcaga tcaaagctca aatcaaccca cggcttacgg ccaagaatcg gacgaggatc
     2461 gccttcgaaa tactcggctg caagatttct tccgccgcac cacaagtgca tgccatccgc
     2521 gatgaccatc ttgcggtggt tgcgcaggtt ggtacgtccg cgttttgggg agcgaaacgg
     2581 cggaacgaag cgaaccacgt cgacacccgc ttgcgcgaag cctttcaaat ccggatagcc
     2641 acccagatag atgccgatac cgtcgatcag cagccgaacc ttgattccag cgcgggcggc
     2701 ctgcttgagc cgttcagcaa tctcgtcgcc gagaacatcg cgcccgaaca caaaggtgga
     2761 gatttccagc gtgcgttttg cgccgtcgat catgcagcgc aacgcgttca gcgcctgcgt
     2821 accgtcctcg tgaatactga gtccctcgta ggccaccggg tcgggcaaac ccatcgccat
     2881 gcccagctgc tgcgcgcggg ctgccgtggc aggtggcgtc ggggccacgg cagcaacccg
     2941 ccgcacaccg cgcgcagcgc gtttggcgac gatcttgcgg ctgccaaatg caagatacag
     3001 tggcagcgcg acgtagggaa gaagcacaat gcccagcacc caggcgatgg ccgcggccgg
     3061 gtgacggcgc tggtgcaggc tgtgggaagc ggccgcatag atagccagtc cgacgacaat
     3121 gagcaggcta tagagaactt cccagtgcct gacaatcaac gcttcgatct gggatatcag
     3181 ctcagtcatc gcgttgctcc agcacccttt cacgcttgcc acggcgcctg tgtgacatga
     3241 aacgcagcag gaatccgccg atcagaaccg ccagcgcagc gtatgccgcc accgcatagt
     3301 ccgaacgcaa aggctttttg tctggtgctg aggggtcttt gaggcccggc ggaaagtgtc
     3361 ggggatccac cggaacaatc tgctgcttgg ccggatcctt cggcaggcgt tgtcggtact
     3421 catcgacgct ggccaccggg caatcgcccc aggcgcgatc cgatggcagt tgcgggacgc
     3481 gtaccagcat gatttcctcg acatcctgcc tgagcagttc ttcgtagggc gtcagttcgc
     3541 gagtggcact gcccttgagc agtcgcagga gatcggcgcc cgcctgttcg aatgcaatcg
     3601 ccggaaaaag ccgggtctga tcttcggtca gataatcaaa gatcgccttg cctttgctca
     3661 agctgccggt cgtcgccgcg accacgggca gcccggcaat cgccttcagc cagctgctcg
     3721 ggcccagaga cggaacgcgc cagccgatgg tgcgcagcgt cgaaatgcag attccagtgc
     3781 aattggcggc ggcgtgatgg taggcaaact ggtgtcgata gaactggttg aatacgcgag
     3841 ccagcgccga gctgaggtgt agcgccgtac gctcatcgcg caaggtggcg accagcaggc
     3901 aggagggccg ataccaggcc tgaccgccat tcagatcagc caggtagcat tccagcggca
     3961 acatggcggc gatgatgcct ttctcgcttt ccgtttcaag ggtatagaag ttggccatca
     4021 gccagtcgtg catttgcccc tgtgaaccaa cgcgtcccgt aaacagcccg aaatgtccgc
     4081 cgtgtgcttc atcgtcatcg ccttgcgcgc cgttgagcat gatgccgagc agcgggctgc
     4141 cggcacgatg ccgccgagaa tcggcatcgc gttgccatat gacctgggtt gtgaagttgc
     4201 tctgtgcacc tccgcgcggc tcactgcgaa cgaatgcgcg tattgcttcc ggggtagcgg
     4261 cgatgggttt gaatgcggca tccgggtcaa gccggaaatc tgccggccag atgctgcgcg
     4321 cgacgaatcg gccgccttca gtttttccac gcaggcgcaa ctcgcgctgc gcgaagaagt
     4381 cagtgctgtc ggcgttgtaa tacgaccggt ttgattcaag ccgtggcgtc aaggaaaagc
     4441 cggtcagtcc cccggcaaag cgcagattct cacccttggc gtcgatccac gcctgctcga
     4501 tgagctcggg cgagccgatc cagacaaggt ccggataatc gcgcgccgaa cccggcagtg
     4561 tcaatgcatt ccagcccagc agagcctcgg tggcagacaa tcgaggatcg agtccttcaa
     4621 tcgggcgccc atgtgctggc acagcaattg tttcctcgcg aaagaaccac cgcgcctgcg
     4681 gaatggtggc actatcagca cggtagccca tcgtgaccat aaaatcggcg gcggggaaca
     4741 agccatatgc cggtgaagtc acatgggcat cggttgattc gataaccatc agggtagtgg
     4801 caagagtgtt ttgctcgaat acatcgtcct attctagcgt gtggtggggg tttttgattt
     4861 cggcagatca acgcaaccac ctataacctg cccgaacttg gtgcactcgc cactctcaac
     4921 gccatcggcg aaagccttca tccatgtatc acggagaaaa attcaacgcc tggacgcatt
     4981 ttgtaggcgc cgtgctttcg attgcgggag cggtggtgct gatcagcagg gcgtctgcga
     5041 cgggtgatcc gtggaagatc gtaagcgcgt ccgttttcgg cgcatcgctg atccttctct
     5101 atagcacctc gacgctctat cacagcctgc gcggacgggc caagatcatc ctgcgcaaac
     5161 tcgaccacct gacgatctac ctgctgatcg ccggaaccta cacacccttc tgcctggtca
     5221 ccttgcgcgg gccctggggc tggtcgctgc tcggcgtcgt gtgggggctg gccgtcgtcg
     5281 gcatgctgca ggaaatcaag ccccgctcgg aggctcgaat cctgtcgcta gtcatctacg
     5341 cggtcatggg ctgggtggtt ctcgtcgcga tcaagccctt gcttgagcag ctggaaaccg
     5401 ccggcttcgt gtggcttgcc agcggcggac tgctctatac cctgggcatc gtgttctacg
     5461 ccaatgacga gcgcttcaag cactggcacg gcatctggca tctgtttgtc atcggaggca
     5521 gtctggcaca atacatcgct gtcgccggct acgtcatata gaagcacagc ctcccctgtg
     5581 cgatcaatag cctttgctga agagggtgcc actgcatttg gagcaaggtg gtacatgacc
     5641 cgtgtgcttg agttgcatcg actgtccgca tgccttgcag gtcaatgtgc cggcggatgt
     5701 catctcgccg gttttgtacg taagggtttc ctttgtcttc tcactcaggg agcgcaaggc
     5761 attgccggtg aattccatca cgttcgcgat ggaagaaagc gcgcctgcac ccaatctgga
     5821 cgggttgaac cgctcttttg cttcttcacc cagttgttga gcgtccgata tggtctgatc
     5881 caggtcgcgg gaaaggtatt tcttcagttc ttcgccttgt tccgcgctga attccccgag
     5941 cgcggtcatc tgcttatggg caatatccag cgcttcggtc atcgattcgc gcccgcgcgc
     6001 ctttccagcc aggtagagtt cgttgaattt gctggccagc ttgtcgtact gttgcgccaa
     6061 tctggccttg tcctgatcgt tgttttccat ggcaattctc ctgaaaacgc ggcatagaag
     6121 gcaacaccgc aacgcctgac gaatgatggg ggctgacctg ccccgagaat tcgggaatcc
     6181 agcgagcctc gataggggaa acccataggc attatggccc aaggggtcga tctcgccaag
     6241 aacgttattt gttatcagct gcgcctgtca tcttcagggc agtcagcggg catcatgtca
     6301 aggtgcttgg caggacacct ggcttcagac atccgcggga tagtgaaacg tcggtagctg
     6361 ccgccagctc gcggcaaagc ggcgattcgc tataatcgtc caattcacga aaaggattgc
     6421 catgcgtttc gaaggttcag aacagtacgt cgccacgcgc gatctgacgc tcgccgtcaa
     6481 tgccgccatc accctgcaac gccctctgct gatcaagggt gagcctggca ccggcaagac
     6541 catgcttgcc gaggaagtgg ctggcgcgct cgggttgccg cttttccagt ggcatatcaa
     6601 gtcgacgacc aaggcacagc agggcctgta tgagtacgat gccgtctccc gtttgcgcga
     6661 ctcgcagttg ggtgatgcca gggtggccga catcgccaat tacatcgtca agggtgtcct
     6721 gtgggaagct ttcaactgcg atacgccgtc ggtggtgctg atcgacgaaa tcgacaaggc
     6781 cgacatcgaa ttccccaacg acctgctgcg cgaactcgac cgcatggagt tcttcgttta
     6841 cgagacacgg caactggtca aggccaggca tcggccgatt ctgttcatca cctcgaacaa
     6901 cgagaaggaa ctgccggacg ccttcctgcg ccgctgcttc ttccactaca tcaagtttcc
     6961 ggacaaggag acgatggtca agatcgtcga cgtgcacttt ccggggctga agaagaatct
     7021 gctgcgcgag gcgcttgagg ttttcttcga attgcgcgaa atccctgggc tgaaaaagaa
     7081 gccatccact tcggaactcc tcgactggct caagctgctg ctggccgaag acatcccgcc
     7141 cgaggccttg cgcagccagg atcagaaggt tgccatcccg ccgctgcacg gcgcactgct
     7201 caagaacgag caggacgtgc atctgtttga gcggctgatc ttcatggcgc gtcagaaccg
     7261 ttagggcagg gttcggcccg aatgctgatc gacttcttcc tccacctcaa ggccagcaag
     7321 ctcccggtat cgaccaagga gtttctggtg
//
LOCUS       DAORPM010000112         7282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2618_length_7282_cov_8.959319, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000112 DAORPM010000000
VERSION     DAORPM010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2618_length_7282_cov_8.959319"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLD53_06800"
     CDS             complement(<1..431)
                     /locus_tag="PLD53_06800"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRC60657.1"
                     /translation="MPNAPINLNADLGEGFGPWSMGDDEAMLDIVSSANIACGGHAGD
                     PETMRRTVRLAKARGVSIGAHPSYPDLQGFGRRAMALTSAELEGQIAFQVGALTGIAT
                     LEAARVTHVKPHGALNNLACIDRGVADTLCRAIRAIDRNLIL"
     gene            complement(412..1455)
                     /locus_tag="PLD53_06805"
     CDS             complement(412..1455)
                     /locus_tag="PLD53_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRC60658.1"
                     /translation="MIEILDCNGYATVQDLGRPGYRHLGVPLSGALDSGLLQIANALA
                     QNPVSAAAIEMRLIGPRLRTRMPLIISLAGSVDGVIESTSGDRRPARAWASHRLLAGD
                     TLKLGPVHSGVAYLGIAGGIDVPPVLGSRSTYERAHFGGFEGRRLAAGDRIEIVRAGQ
                     PERGEENSRRELRLPEPPLLKDRAIRVLAGPHSEHFTDKARARLTSEEFVVSRQADRM
                     GLRLEGSRLDHDPQLGSDIISDAVTPGAIQVPGDGQPIVLLADCQTVGGYPVIATVIS
                     ADLPRLGHAMPGQSLRFVKVDYQQAVVARGCAATELTDRIAGIRPNHVELDSEALFAA
                     NLIDGVFDAQRAY"
     gene            complement(1452..2180)
                     /gene="pxpB"
                     /locus_tag="PLD53_06810"
     CDS             complement(1452..2180)
                     /gene="pxpB"
                     /locus_tag="PLD53_06810"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRC60659.1"
                     /translation="MKPRILDLGDTALTLELGDRVGLALNARVLASRDALGKLRGITD
                     VVPTYRSLTVHFDPAIVDREEVVAKLTHAAESSFSREASLDTTGAQWRIPVLFGGTHG
                     PDLAAVARIAERSEDAVVEALCSLQLRVFMIGFLPGFPYLGELPEWLRLPRLSTPRNA
                     VPPHSVAIAGAQAAIYPWSSPGGWHLIGQTPLRMFDLAVPERPALLVAGDTVQFTPVN
                     LDEFARLTSAEEAGQLRREQFFTS"
     gene            complement(2177..3475)
                     /locus_tag="PLD53_06815"
     CDS             complement(2177..3475)
                     /locus_tag="PLD53_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRC60660.1"
                     /translation="MDLAITFGLILALFALLGSGIWIGLALLGVGWLGMELFTARAAG
                     DAMAVTIWGASSSWTLTALPMFLWMGEVLFRTRLSSDMFKGLSPWLEKLPGRLLHVNV
                     IGCTIFAAVSGSSAATCATIGKITLPELKKRGYPEDITIGTLAGAGTLGLLIPPSIIM
                     IVYGVAAEVSIAKLFIAGVLPGILLASLFMGWIVVWALLHPGAIPEATEKTSVGQKLF
                     ASRHLIPVLLLIGLILGSIYTGVATATEAAALGVVGSLVLAATQGSLSWATFRDSLLG
                     ATRLYCMIALILAGSAFLTLSMGYMGLPRHLAEWIGSLGLGQFGLLFGLMIFFIILGC
                     FLDGISMVVLTIAVIQPTVVAAGIDLIWFGVFLVVVVEMAQITPPVGFNLFVIQGMTG
                     KQLPWIARTAMIPFALMMVASGLLYVFPAIITWLPQRMAG"
     gene            complement(3491..4012)
                     /locus_tag="PLD53_06820"
     CDS             complement(3491..4012)
                     /locus_tag="PLD53_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRC60661.1"
                     /translation="MTVRRALDRVYGAAAWMAALFMIGTLVMILLSIGGRLLNFHVRG
                     TDAYAGYSMAAAAFLALAHTFKRGEHIRVTLLLDHAGRGGRHILELWSHVAGLLCAAV
                     LAWFSVRLVWQSLQFNDISQGTDATPLWIPQLGMAIGSVIFTVAVAEELIATLRGGKL
                     RVRTAADEAARSE"
     gene            complement(4026..5000)
                     /locus_tag="PLD53_06825"
     CDS             complement(4026..5000)
                     /locus_tag="PLD53_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRC60662.1"
                     /translation="MNTRLCTLIAAAAIGFAAPTVSAQTTWDMPTPYPAANFHTENIM
                     QFAGEVDKATAGKLKITVHAGGSLFKANEIKRALQGGQAQIGEIILGGYANENPLFGL
                     DNVPFLATSYPEAKKLAVTQKPALDAMLAKQGLKMLFAVPWPPQGIFSANPINSAADL
                     KGVKWRAYSPQTSRIAELVGAQPVTIQAAELSQALATGAVAAFMTSGATGYDSKVWEQ
                     IKYFYDAQAWLPKNAVLVSQKAFDALDKATQAAVLKAAADAESRGWKTSEDKTKFYLE
                     QMAKNGMSVTPPSAALNTDLKKVGDTMIAEWLKTAGAEGQSIVDAYKK"
     gene            5125..5685
                     /locus_tag="PLD53_06830"
     CDS             5125..5685
                     /locus_tag="PLD53_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRC60663.1"
                     /translation="MENSKSENKSVGRSTITKPGVSRIVSAEHLVSEKCPELSELEFG
                     LIIASHAFGRWMTRCMAGAGVKDMTETEILVVHHVNHRDREKKLADICFVLNIEDTHV
                     VSYALKKLANLGLVLSNRRGKEVFWSTTEAGQALCERYRQVREACLMPGFTGAQDENQ
                     RIGDLARFLRTLSGRYDQGARAASSI"
     gene            5793..6791
                     /locus_tag="PLD53_06835"
     CDS             5793..6791
                     /locus_tag="PLD53_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRC60664.1"
                     /translation="MPELVCPAGSLPALKAAVDNGADTVYFGYKNDTNARNFAGLNFD
                     HKAMLEGIRYAHSRGKHVLMAINTFPQPGRESDWQDAVDGAADLGVDAVILADIGLLD
                     YARTRHPDLRLHLSVQGSATSYEAVNFAHREFGVRRAVLPRVLTLAQVEQVIRNTPVE
                     IEIFGFGSLCVMNEGRCWLSSYATGESPNTVGACSPAKYVKWERSPGQMSTRLNGILI
                     DRCADNEPAGYPTLCKGRFEVNGETYYALEEPTSLNVLEILPEIVRIGVSAIKVEGRQ
                     RSPTYVAQVTRTLRAALDELGQDFERFAVKPAWQAALGKVSEGIQATLGAYNRPWR"
     gene            6878..>7282
                     /locus_tag="PLD53_06840"
     CDS             6878..>7282
                     /locus_tag="PLD53_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRC60665.1"
                     /translation="MKLALGPLLYFWPKHEVLEFYAEISQVDAIDIIYVGEVVCSRRQ
                     QLRTSDWIGLARDLTDAGKEVVVSAQALLESESDLKALRRLMTELTEMHGCKVEANDL
                     GAVNLAAGRPFVAGPHLNIYNEATLATFARYGM"
BASE COUNT         1544 a         2153 c         2198 g         1387 t
ORIGIN      
        1 agaatcagat ttcgatcgat tgcccgaatc gcgcggcaaa gcgtgtcagc gacaccacgg
       61 tcgatgcagg caagattgtt cagtgcgccg tgtggcttga catgggtcac ccgggccgct
      121 tcgagcgtgg ctattccggt gagcgccccg acctgaaatg cgatctgtcc ttcgagctcg
      181 gccgaagtga gcgccatggc acggcgaccg aagccctgca gatcaggata gctcggatgg
      241 gctcctatcg aaacgccacg cgcttttgcc agacgaaccg tgcggcgcat tgtttccgga
      301 tcaccggcat gccctccaca agcgatgttg gccgaactga cgatatcgag catggcttcg
      361 tcgtcgccca tgctccaggg accaaaacct tcaccgaggt cggcgttgag gttaataggc
      421 gcgttgggca tcgaacactc cgtcaataag gttggccgcg aaaagcgcct cggaatccaa
      481 ctcgacatgg ttgggccgaa tgccggcaat gcgatcagtc agctccgttg ctgcgcagcc
      541 acgggcgaca acggcctgct gatagtctac tttgacaaat cgcagtgatt gccccggcat
      601 ggcatgaccc aagcgtggca ggtcggcact gatcacagtg gcaatgactg ggtagccgcc
      661 gaccgtctga caatcggcca gaagaacgat gggttggcca tcacctggaa cctgaatcgc
      721 gcctggggta accgcgtccg agatgatgtc ggaaccaagc tgcggatcgt gatccaggcg
      781 agatccttcg agccgtagcc ccatgcggtc ggcctgccgg ctgacaacga actcctcgct
      841 cgttagccgg gcgcgcgcct tatcggtgaa atgttccgag tgcggcccgg ccagcacacg
      901 tatcgcgcga tctttcaaca ggggcggctc aggtagtctg agctcgcgac gcgagttctc
      961 ttccccccgc tctggctgtc ctgcacgcac tatctctatc ctgtcgcccg cagcaagtcg
     1021 tcggccttca aaaccaccaa agtgcgcgcg ctcgtaggtc gagcggctgc caagcaccgg
     1081 cggcacgtca attcctcccg cgatgccaag ataggcgacg ccgctgtgca ccgggcccag
     1141 cttcagcgta tcaccggcga gcagccggtg gctggcccag gcgcgggcag ggcgacgatc
     1201 gccgcttgtc gattcgataa ccccgtcgac cgatccggcg agcgaaatga ttaacggcat
     1261 cctggtgcga aggcgcggcc cgatgagacg catttcaatc gctgcagcag acacagggtt
     1321 ctgcgcaagg gcgttagcaa tttgcagcag cccggaatcc agtgcccccg acagtggcac
     1381 gccaagatga cggtagcccg gacgacccag atcctgcacc gtggcgtagc cgttgcagtc
     1441 cagaatctcg atcatgatgt gaagaactgc tctcgacgca attgccccgc ttcttctgcg
     1501 gaagtgagtc gcgcgaattc gtcaaggttg actggtgtga attgaacagt gtcgccagca
     1561 acgagcaggg ccggtctttc aggcacggcc aagtcaaaca tgcgcaaggg ggtttgaccg
     1621 atcaggtgcc aacctccggg gcttgaccag ggatagatgg ctgcctgggc gccggcgatg
     1681 gccacgctgt ggggtggaac ggcgtttcgc ggggtggata gccgcggtaa tcgcagccat
     1741 tcaggtaatt cgcccagata gggaaatccc ggcagaaatc cgatcatgaa aacgcgcagt
     1801 tgcaggctgc atagcgcctc aaccacagca tcttcgctcc gctcagcgat gcgggcgacc
     1861 gcggccaaat ccggcccgtg cgtaccgccg aacaagacag gtatgcgcca ttgagctccc
     1921 gtcgtatcca aagaagcttc tcgcgagaag gacgactcgg cggcgtgggt caactttgct
     1981 accacttcct cccgatcaac gattgccgga tcgaagtgca cggtcagcga acgataggtc
     2041 ggcacgacgt ccgtgatgcc gcgcaatttg ccaagcgcat cccgggacgc caacacgcgt
     2101 gcgttcaaag ccagacccac ccgatcgccc aattccaagg tcagcgccgt gtcaccaaga
     2161 tcaagaattc gtggtttcat ccggccatgc gctgcggcag ccaggtgatg attgccggga
     2221 agacgtagag caatccgctg gcaaccatca tcagtgcgaa agggatcatc gccgttcggg
     2281 caatccacgg taattgcttg ccggtcatcc cctgaatgac aaacaggttg aagccgacgg
     2341 gcggcgtgat ctgcgccatt tcgacgacca cgacaagaaa tacaccgaac cagatcaggt
     2401 cgatgcctgc ggcgacgacg gtcggctgaa ttacagcgat cgtcagcacc accatcgaga
     2461 tgccatcgag aaagcagcca agaattatga agaagatcat aagcccgaac agcagaccaa
     2521 actggcccag gcccaggctg ccgatccact cggccagatg gcgtggcaac cccatatagc
     2581 ccatcgatag cgtcaggaac gcgcttcccg cgaggatgag cgcgatcatg caatatagcc
     2641 gggtcgcgcc aagcagacta tcgcgaaaag tagcccacga aagcgaaccc tgggtggctg
     2701 ccagtaccag actgccgact acacccagcg cggcggcctc ggtggctgtg gcgacaccgg
     2761 tgtatatgga ccccaggatc aggccgatca acagcagcac gggtatcaaa tgacgcgagg
     2821 caaagagctt ctggccgacg ctggtttttt cagtcgcctc cgggatggcg ccgggatgaa
     2881 gcagcgccca gacgacgatc cagcccatga acaggctggc cagaaggatc cccggcagca
     2941 cgccggcgat gaacagcttg gcgatggaga cttcggcggc cacgccgtag acaatcatga
     3001 tgatcgacgg cgggatcagc aggcccagcg tgccggcacc ggccagagtg ccgatcgtga
     3061 tgtcctcggg gtatccgcgt ttcttgagtt ccggcagagt gatcttgccg atcgtggcgc
     3121 aggttgccgc agaggagccg gagacggcgg caaagatggt gcagccgatg acgttgacat
     3181 gcagcaggcg gcctggcagt ttttcgagcc acggcgaaag tcccttgaac atgtcggacg
     3241 aaaggcgcgt gcgaaacagc acttcgccca tccagagaaa catcggcagt gcggtcagcg
     3301 tccaggatga cgaagccccc cagatcgtga cggccatggc gtcgccagcg gcacgagccg
     3361 tgaacagttc catccccagc cagccgacgc cgagcagcgc cagcccgatc cagattccac
     3421 tgccgagcaa tgcgaagagg gcgagaataa gtccgaaagt gattgccaga tccattgctt
     3481 tgtctccaga ctactcgctg cgtgcggctt cgtcggcagc agtgcggacg cggagtttcc
     3541 cacctcgcag cgtggcgatc aattcttcag cgacggccac ggtgaatatc acgctgccga
     3601 tagccatgcc caattgcgga atccatagcg gagttgcatc cgttccttgc gagatgtcat
     3661 tgaattgcag ggactgccaa accagacgta cggaaaacca ggccagcaca gcagcacaga
     3721 gtaatccggc tacgtgactc cagagttcca gaatgtgtcg gccgccacgg ccggcatgat
     3781 cgagcagcag agtcacgcgg atgtgttcgc cgcgtttgaa cgtatgtgcc aaagccagaa
     3841 atgccgccgc cgccatgctg tagccggcgt aggcatcggt tccccgcaca tggaaattca
     3901 gcagacgacc gccgatcgaa agcaggatca tgaccaatgt gccgatcatg aacagcgctg
     3961 ccatccaggc tgcagcgcca taaacccggt cgagcgctcg ccgaactgtc acgagagcct
     4021 cgcgctcact tcttgtaggc gtcgacgatg gactgccctt cagcaccggc ggttttcagc
     4081 cattccgcga tcatcgtgtc gccgactttt ttcaggtcgg tgttcagtgc cgcggacggc
     4141 ggtgtaacgc tcatgccgtt cttggccatt tgttccaaat agaacttggt tttatcctcg
     4201 ctggtcttcc agccgcgcga ctcggcatcc gcggcggcct tcagaacagc cgcctgagtc
     4261 gccttgtcga gagcatcgaa agctttctga gaaaccagta cggcgttttt tggcagccag
     4321 gcctgggcat catagaaata cttgatctgt tcccacacct tgctgtcgta gcctgtcgcg
     4381 cccgaagtca tgaaagcggc cacggccccg gtggccagcg cctgcgagag ctccgcggcc
     4441 tggatggtca ctggctgtgc tccgaccagc tcggcgatgc gcgaggtttg cgggctgtag
     4501 gcgcgccact tgacaccctt cagatcggcc gcgctgttga ttgggtttgc cgaaaatatg
     4561 ccctgaggtg gccagggtac ggcgaacagc atcttcaggc cctgtttcgc cagcatcgca
     4621 tccaatgccg gcttctgcgt tacggcgagc ttcttcgctt cggggtaaga ggtggccaga
     4681 aatggaacgt tgtcgagacc gaacaacgga ttctcgttgg catagccacc cagtatgatt
     4741 tcaccgatct gcgcttggcc gccctgtaac gcgcgcttga tttcattggc tttgaaaagc
     4801 gagcccccgg cgtgaacggt gatcttcaac ttgccggcgg tggctttatc gacctcgcca
     4861 gcgaattgca tgatgttttc agtatgaaag ttggctgctg gataaggggt aggcatgtcc
     4921 cacgtggttt gagccgaaac ggttggggct gcaaagccga tagcggcggc tgcgatgagc
     4981 gtgcaaaggc gtgtattcat caccggactc ctcgttaaat taaggatcaa agggattttg
     5041 cagttacgac gttaataata tatatacact ttgtcaatat aatgatgaaa tagcacaatc
     5101 gtatttcact tggaagttgc tgccatggaa aacagtaagt cggaaaacaa atcagtcggg
     5161 cgttcgacga tcactaagcc cggggtcagt cgcatcgtct ccgccgagca tctggtttcg
     5221 gagaagtgcc ccgagctttc cgaacttgaa ttcgggctca ttattgccag tcatgccttc
     5281 ggacgctgga tgacgcgttg catggccggc gccggagtaa aggacatgac cgagacggag
     5341 atactcgtcg tccatcacgt caatcatcgt gaccgcgaga agaaactggc tgacatctgt
     5401 ttcgtgctga atatcgaaga cacccatgtc gtttcctacg cgctgaaaaa gctcgccaat
     5461 ctgggcttgg ttttgagcaa ccgacgcggc aaggaagtgt tctggtcgac caccgaagcg
     5521 gggcaggccc tctgcgaacg ctatcgtcag gtacgcgaag cctgcctgat gccgggcttc
     5581 accggtgcgc aggacgagaa ccagcgcatc ggcgatctgg cacgctttct gcgcaccttg
     5641 tcaggtcgct acgatcaggg tgcgcgggcg gcctcttcga tttgaggaat agcccgcaag
     5701 ggcccggaaa ttgatcaaag tcatgcgctt tgaacgcgcg ctgggtatac ttgcggcttc
     5761 gctttcagag aattctcatg cggcacgaga aaatgcccga actcgtttgc ccggccggta
     5821 gcctcccggc gctcaaggcc gcggtcgaca acggtgccga caccgtctat ttcggctaca
     5881 agaatgacac caatgcgcgc aatttcgccg gcctgaattt tgaccacaag gcgatgctcg
     5941 aggggattcg ttacgcccac tcgcgtggca agcatgtgct gatggcgatc aatacctttc
     6001 ctcaaccggg tcgcgagtcg gactggcagg atgccgtgga cggcgcagcc gatctgggcg
     6061 tcgatgcggt cattctggcc gatatcgggc tgctcgatta cgccagaacg cgtcaccctg
     6121 atctgcgtct tcatctctcg gtgcagggct ccgcaaccag ctacgaagcg gtgaatttcg
     6181 cgcatcgcga gttcggcgtg cggcgtgccg tgctgccacg cgtgttgacg ctggcccagg
     6241 tcgaacaggt gattcggaac acgccggtcg agatcgaaat cttcggcttc ggtagcttgt
     6301 gcgtgatgaa cgaggggcgc tgctggcttt cgtcctatgc cacgggcgaa tcgccgaaca
     6361 ctgtcggtgc ctgctcgccg gccaagtatg tgaaatggga gaggtcgccg ggacagatgt
     6421 ccacgcggct caacggtatt ctcatcgacc ggtgcgccga caacgaaccc gctggctacc
     6481 cgaccttgtg caagggacgc ttcgaggtca acggcgaaac ctattacgcg ctggaagagc
     6541 cgaccagttt gaacgttctg gagatcctgc cggagattgt gagaatcgga gtttcggcga
     6601 tcaaggtcga agggcggcag cgcagcccga cctatgtcgc ccaggtaacg cgcaccttgc
     6661 gcgccgcgct cgacgaactg ggtcaggatt tcgagcgctt cgcggtcaag ccggcctggc
     6721 aggccgcgct cggcaaggtc tcggaaggta tacaggctac gctgggcgct tacaaccgtc
     6781 cgtggcgata gagtatgaag cgcctcaagg ctgagatcac tggggcacag agaagcggga
     6841 atgcccagag atgtccgtgt cgaaaaaggc tttgcccatg aagctcgctt taggcccctt
     6901 gctctacttc tggccgaaac atgaagtgct tgagttttat gccgagattt cgcaggtcga
     6961 cgccatcgac atcatctatg tcggcgaggt ggtctgctcg cgccgtcagc aactgcgcac
     7021 cagcgactgg atcggtctgg cgcgcgatct gaccgacgcc ggcaaggagg tcgttgtctc
     7081 cgcccaagca ctactcgaat ccgaaagcga cctcaaggca ctgcgccgcc tgatgacgga
     7141 attgacggaa atgcacggct gcaaggtcga ggccaacgat ctgggtgcgg tgaatctcgc
     7201 agccggaagg ccgttcgtcg ccgggccgca cctcaacatt tataacgaag cgacgctggc
     7261 gaccttcgcc cgttacggca tg
//
LOCUS       DAORPM010000113         7177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2679_length_7177_cov_14.819573, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000113 DAORPM010000000
VERSION     DAORPM010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2679_length_7177_cov_14.819573"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1147)
                     /locus_tag="PLD53_06845"
     CDS             complement(23..1147)
                     /locus_tag="PLD53_06845"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRC60666.1"
                     /translation="MSDVATLSRLTPAVSQLPVDWYFDEKVFELEKKLIFDAGPGYVG
                     HQLMVPEAGDYRSLEWKDHGQMLLNGGNEGANAGNWQMSNVCRHRQAVMLQGSGKLQG
                     NIVCPIHRWTYDTGGELIGAPHFPQNPCLNLNKAKLENWHGLLFKGPRSANADLAGMN
                     VGSGLDFAGYKLDHVEMHECNYNWKTFIEVYLEDYHVAPYHPGLGNFVSCDDLTWQFG
                     DWYSVQRVGITSLKKSGSKVYERWQKAVLDIYGTEGKTPEHGAIWLTYFPNIMVEWYP
                     HVLVVSTLIPQAVNKTLNVVEFYYPEEIVDFERDFIEAERAAYMETAIEDDDIGERMD
                     RGRYALMKEGRNEVGPYQSPMEDGMQHFHEFYRRIMQVHI"
     gene            1252..1491
                     /gene="xseB"
                     /locus_tag="PLD53_06850"
     CDS             1252..1491
                     /gene="xseB"
                     /locus_tag="PLD53_06850"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRC60667.1"
                     /translation="MDQSPIADMKFETALADLESIVSSMEGGKLELEDSIAAYRRGME
                     LMKHCQAQLTHAEEQIRILENGQFKDVDRSTLEAQ"
     gene            1488..2381
                     /locus_tag="PLD53_06855"
     CDS             1488..2381
                     /locus_tag="PLD53_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRC60668.1"
                     /translation="MSLSPFTEWMAATQNRVETALTQHLPGHDCIPARLHEAMRYATL
                     GGGKRVRPLLAFAAGELSNAAPEKLDIVACAVEMIHAYSLVHDDLPCMDNDDLRRGRP
                     TCHIEFDEPTALLVGDSLQTLAFELLASQPISEPARQLEMIALLGHASGSRGMAGGQA
                     IDLASVGKALNQPELELMHALKTGALIRAAVMLGALAGQALSAEERQNLDRFAKRAGL
                     LFQVVDDILDCTASTATLGKTAGKDEAAAKPTYVSLLGLDAARDYADELRNDALDALA
                     IFGERATRLTQLADFICHRQF"
     gene            2383..4245
                     /gene="dxs"
                     /locus_tag="PLD53_06860"
     CDS             2383..4245
                     /gene="dxs"
                     /locus_tag="PLD53_06860"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRC60669.1"
                     /translation="MNAFSLLETINSPADLRRLDRKQLPQLATELRAFLIDSVSQTGG
                     HLSSNLGTVELTIALHAVFNTPEDRLVWDVGHQCYAHKILTGRRERMSTLRMHNGLSG
                     FPKRCESPYDTFGVGHSSTSISAALGMALAAKHKGEDRKSIAIIGDGAMSAGMAFEAM
                     NNAGVADADMLVILNDNEMSISPPVGALNNILTRLMSGKTFNAAREGARNMLGFAPPL
                     LELARRAEEHVKGMIAPGTLFEEFGFHYYGPIDGHDLDALIPTLENLRQLKGPKFLHV
                     ITKKGQGYKLAEADPILYHGVAKFEPGVGIKASKGGKLTYTQVFGDWLCDMAQADSRL
                     VGITPAMREGSGLLRFSIEYPDRYFDVGIAEQHAVTFAAGLACEGLKPVVAIYSTFLQ
                     RAYDQLIHDVALQNLPVIFAVDRGGLVGADGPTHHGTFDLSFVTCIPNMVVMAPADEA
                     ECRRMLSTAYTLDGPSMVRYPRGGGTGTVPEMNLDTLPVGKGEICRAGKEVALLAFGS
                     MVAAALAAGEELDATVVNMRFVKPLDIDLIVELAGNHSLLVSIEENAIIGGAGSEIER
                     ALAERGLNVPLLRLGLPDRFIDHGDQTRLLAELGLDKDGILRTVREKCATTNTQ"
     gene            4264..5064
                     /gene="folE2"
                     /locus_tag="PLD53_06865"
     CDS             4264..5064
                     /gene="folE2"
                     /locus_tag="PLD53_06865"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRC60670.1"
                     /translation="MSTPTPTIPDVQNSADTRQLAINKVGIKAIRHPIKVKDKSTGVQ
                     HTIAVFNMYVGLPHNFKGTHMSRFVEILNSHEREISVENFPTMLRDMLVKLEAETGHI
                     EMNFPYFINKTAPISGVQSLMDYDVTFIGDISHGEIAMSVKVVVPVTSLCPCSKKISE
                     YGAHNQRSHVTVTARTNKFVWIEEIVQLVEQEASCELFGLLKRPDEKYVTERAYDNPK
                     FVEDMVRDVAARLNAEVRVDSYVVESENFESIHNHSAYALIEKDKNQG"
     gene            complement(5159..5533)
                     /gene="rplS"
                     /locus_tag="PLD53_06870"
     CDS             complement(5159..5533)
                     /gene="rplS"
                     /locus_tag="PLD53_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRC60671.1"
                     /translation="MNLIEQLEQEEIARLGKTLPDFAPGDTIVVQVKVKEGTRERLQA
                     YEGVVIAKRNRGLNSAFTVRKISSGEGVERTFQTHSPLVASVEVKRRGDVRRAKLYYL
                     RERSGKSARIKEKLTRKVVVAA"
     gene            complement(5561..6325)
                     /gene="trmD"
                     /locus_tag="PLD53_06875"
     CDS             complement(5561..6325)
                     /gene="trmD"
                     /locus_tag="PLD53_06875"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRC60672.1"
                     /translation="MIRFDCVTIFPEMFVAVTQSGITRRALEEQRWVWQGWNPRDFAE
                     NAWRRVDDRPFGGGPGMLMQPGPLERTIEAAKAQQHLAGLSKSRVIYLSPQGRPLTHE
                     RVMQLATGEEGLILLCGRYEGIDERLIERCVDEEISIGDFVLSGGELPAMALIDAVVR
                     QLPGVLGDAASAVEDSFVTGLLDCPHYTRPEEYAGQRVPDVLLSGDHKRIRRWRLKQS
                     LVRTRKRRPDLLAHRVLSAEETQLLTEIVGEEQCGE"
     gene            complement(6322..6819)
                     /gene="rimM"
                     /locus_tag="PLD53_06880"
     CDS             complement(6322..6819)
                     /gene="rimM"
                     /locus_tag="PLD53_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300530.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRC60673.1"
                     /translation="MVVLGRLADPYGMQGWLRLHAFGDDPLAWAEMPVWWISNDGESW
                     RECKLKRLKSHANGLVVLLDGVVDRTAAEAMKGILVGAPREALPTTDEGEFYWSDLIG
                     LDVINNAEERLGKVASLIETGASSVLRVVSDDNTERLLPFVAAVVLTVDKEARLIRVE
                     WGSDW"
     gene            complement(6847..7101)
                     /gene="rpsP"
                     /locus_tag="PLD53_06885"
     CDS             complement(6847..7101)
                     /gene="rpsP"
                     /locus_tag="PLD53_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRC60674.1"
                     /translation="MVVIRLARGGAKKRPFYNMVVTDSRNRRDGRFVERIGFYNPVAS
                     GNAESLRVATDRLAYWQGQGAQLSPTVARLVKQHAAQQVA"
BASE COUNT         1640 a         2283 c         1801 g         1453 t
ORIGIN      
        1 ccctatctgg tgcagaaagc gcttaaatgt gcacctgcat gatccgccgg taaaactcat
       61 ggaaatgctg catgccatct tccatcgggc tctggtaagg gccaacctcg ttacgccctt
      121 ccttcatcaa ggcatagcgg ccgcgatcca tacgctcgcc gatatcgtcg tcttcaatgg
      181 ccgtttccat ataggcggcg cgctcggctt cgatgaaatc gcgctcgaaa tcgacaattt
      241 cttccgggta gtagaactcg acgacattca gcgtcttgtt caccgcctgc gggatcagcg
      301 tcgacacgac cagcacatgc ggataccact cgaccatgat gttcggaaaa taagtcagcc
      361 agatcgcgcc atgctcaggc gtcttgccct ccgtgccata aatatcaagc accgcctttt
      421 gccaacgctc atacaccttg gagccggatt tcttcagcga agtgatcccg acacgctgca
      481 ccgaatacca gtcaccaaac tgccaggtca aatcgtcgca actgacgaaa ttacccagac
      541 cggggtgata gggtgccacg tgataatcct ccagatagac ctcgataaag gtcttccagt
      601 tgtaattgca ctcgtgcatc tcgacgtgat caagcttgta gccggcgaaa tcgagcccgc
      661 tacccacgtt catcccggcc agatcagcat tggccgagcg cggccccttg aagagcaggc
      721 catgccagtt ctccaacttg gccttgttca gattcaaaca ggggttttgc ggaaaatgcg
      781 gcgcaccgat cagttccccg ccggtatcgt aggtccagcg atgaatcggg cagacaatat
      841 tgccctgcaa cttgccggaa ccctgcaaca tgaccgcctg gcgatgacgg cagacattgg
      901 acatctgcca gttcccggcg ttggcacctt cgttgccgcc attgagcaac atctggccgt
      961 ggtccttcca ttcaagggaa cggtaatccc ccgcctccgg aaccatcaac tggtgaccca
     1021 cataccccgg gccggcatcg aagatgagct ttttctccag ctcgaaaact ttttcatcga
     1081 agtaccagtc caccggcagt tgggaaactg ccggggtcaa acgggacaaa gtcgccacgt
     1141 cggacattcc atgcctctag cgggttaaaa gaacggattt tactctcccc acgatttgac
     1201 ctgaagcccc ttgatagagt attttcgcgt gtttccctga agcccgatga catggatcaa
     1261 tcccccatcg ccgacatgaa attcgagacg gccctcgccg atcttgaaag cattgtctcc
     1321 agcatggaag gcggcaagct tgaacttgaa gactccatcg ccgcttaccg ccgtggtatg
     1381 gaacttatga agcactgtca ggcgcaactg acccatgccg aagagcagat ccgcattctc
     1441 gaaaatggcc aatttaaaga cgttgaccgc agcacgctgg aggcccagtg agcctgagtc
     1501 cgtttaccga atggatggcg gcaacgcaaa accgcgttga aaccgccctc acccagcatc
     1561 tgccgggaca cgactgcatc ccggcccgcc tgcatgaggc catgcgttat gcgacgctgg
     1621 gcggcggcaa acgcgttcgt ccattgctcg ccttcgccgc cggcgaactg agcaacgcgg
     1681 cgccggaaaa actcgacatc gtcgcctgcg ccgtcgagat gatccacgcc tattcgctgg
     1741 tccatgacga cctgccgtgc atggacaatg acgacctgcg ccgcggccgg ccaacctgcc
     1801 acattgaatt cgacgaaccc accgccctcc tggtcggtga cagcctgcaa acgctggctt
     1861 ttgaactatt ggccagccag cccatttccg aaccggcccg ccagctggaa atgatcgccc
     1921 tgctcggtca cgccagcggc tcgcgcggca tggccggcgg tcaggcgatc gacctcgctt
     1981 cagtcggcaa ggcgctcaac cagcctgagc tggagctaat gcatgcgcta aagaccggcg
     2041 ccctgatccg tgccgccgta atgctgggcg ccttggcggg acaagcgcta tcggccgagg
     2101 aacgccagaa tctggaccgt tttgccaaac gcgccggcct gcttttccag gtggtcgatg
     2161 acattcttga ctgtacggcg agcacggcca ccctcggcaa aacggccggc aaggacgaag
     2221 ctgctgccaa gccaacttat gtcagcctgc tcggcctcga tgcggcgcga gactacgccg
     2281 acgaactacg taatgacgca ctcgacgccc ttgccatttt cggcgagcgc gccacccgcc
     2341 tgacgcaact ggccgacttc atctgtcacc gacaattctg aaatgaacgc cttctccttg
     2401 cttgaaacca tcaacagccc ggccgacttg cgccgcctgg atcgcaagca attgccgcaa
     2461 ttggcgactg aactgcgcgc ttttctgatc gactccgtct cgcaaaccgg cggccatctc
     2521 tcatccaatc ttggcaccgt cgagctgacc atcgccctgc acgccgtctt caacaccccg
     2581 gaagatcgcc tggtctggga tgtcggccat cagtgctatg cccacaaaat actgaccggg
     2641 cgccgcgaac gcatgagcac gctgcgcatg cacaacggcc tttccggctt tcccaagcgt
     2701 tgcgaaagcc cgtacgacac ctttggtgtc ggccattcat caacttcgat ttccgctgcg
     2761 cttggcatgg ccctggccgc caaacataaa ggtgaggacc gcaaatccat cgccatcatc
     2821 ggcgacggcg ccatgtcggc gggcatggcc ttcgaagcca tgaacaatgc cggcgttgcc
     2881 gatgccgaca tgctggtcat cctcaacgac aacgaaatgt cgatctcgcc gccggtcggt
     2941 gcgctcaaca atatcctgac ccgcctgatg tccggcaaga ccttcaacgc cgcgcgcgaa
     3001 ggcgctcgca acatgctcgg cttcgccccg ccgttgctgg aacttgcccg ccgcgccgaa
     3061 gagcacgtca agggcatgat cgcgccgggc acgctgtttg aagagttcgg gttccattac
     3121 tacggcccga tcgacggtca cgatcttgat gccctgatcc cgacgctgga aaacctgcgc
     3181 cagctcaaag gcccgaaatt tctccacgtc atcaccaaaa aaggccaagg ctacaaactg
     3241 gccgaagctg accccattct ttaccacggc gtcgccaagt tcgaaccggg cgttggcatc
     3301 aaggccagca aaggcggcaa actaacctac actcaggttt tcggcgactg gctgtgcgac
     3361 atggcccagg ccgactcccg actggtcggc atcaccccgg ccatgcgtga aggctccggc
     3421 ctgctgcgct tttccatcga atatccggat cgttattttg atgtcggcat cgccgagcaa
     3481 cacgctgtca ccttcgccgc cggcctcgcc tgcgaaggcc tcaaaccggt cgttgccatc
     3541 tattcgactt tcctgcaacg ggcctacgat caattgattc acgacgtcgc cctacagaat
     3601 ttaccggtca ttttcgcagt tgaccgtggc ggtctggtcg gcgccgacgg cccgactcac
     3661 cacggcacct tcgatctatc cttcgtcacc tgcatcccca atatggtggt catggcgcca
     3721 gccgacgaag ccgaatgccg tcgcatgctc tccaccgcct acacgctgga tggacccagc
     3781 atggtccgct acccccgcgg cggcggcaca ggtacggtgc cagagatgaa tctcgacacg
     3841 ctgccggtcg gcaaaggtga aatctgccga gccggcaaag aggtcgccct gctcgccttt
     3901 ggcagtatgg tcgccgcggc actggctgcg ggtgaagaac ttgacgccac agtggtcaac
     3961 atgcgcttcg tcaaaccact cgatattgat ctgattgtcg aactcgccgg gaaccattcg
     4021 ctgttggtca gcatcgaaga gaatgcaatc atcggtggcg cgggttcaga aatcgagcgg
     4081 gcgcttgcag aacgtgggtt aaatgtgccg ctgttgcgcc ttggccttcc ggaccgcttt
     4141 atcgatcacg gcgatcaaac ccggctattg gccgaactcg gcctcgacaa agatggcatc
     4201 ttgcgcactg ttcgtgaaaa gtgcgccaca accaacacac aataattcat taaagaacag
     4261 cccatgagca ccccaactcc aaccatcccc gacgttcaga actctgccga cacccgccaa
     4321 ctggcgatca acaaggtcgg catcaaagcc attcgccatc cgatcaaggt caaggacaaa
     4381 tcgaccggcg tgcagcacac cattgctgtt ttcaacatgt acgtgggcct gccgcacaat
     4441 ttcaagggca cgcacatgtc gcgctttgtg gaaattctca atagccacga acgtgaaatt
     4501 tcggtcgaaa acttcccgac catgctacgc gacatgctcg tcaaactcga agctgagacc
     4561 ggccacatcg agatgaattt cccgtatttc atcaacaaga cggccccgat ctccggggtt
     4621 caaagcctga tggattacga cgttaccttt attggcgaca tctcccacgg ggaaattgcc
     4681 atgtcggtca aggttgtcgt cccggttacc agcctttgcc cctgctccaa gaaaatttcc
     4741 gagtatggtg cgcacaacca gcgctcacac gtcaccgtca ccgcccgtac caacaaattt
     4801 gtctggattg aggaaattgt ccagttggtc gaacaggaag cctcctgcga actcttcggc
     4861 ctcctcaaac gtcccgatga gaaatatgtc accgaacgcg cctacgacaa ccccaagttc
     4921 gttgaggaca tggttcgcga cgttgccgcc cgcctgaatg ccgaagttcg agtcgactca
     4981 tatgtcgtcg aatccgagaa tttcgagtcc atccacaatc actctgccta cgcgctgatc
     5041 gaaaaggaca aaaatcaggg ttgatcacga cggctgcggt caacgccctt attcgggtaa
     5101 aaaaccaccc atgaaaaaag ggcgccgagg cgcccttttt gctatccgaa gctatcgctt
     5161 atgcagcgac aacaaccttg cgggtcagct tttccttgat acgtgcggac ttgcctgaac
     5221 gctcgcgcag gtaatacagc ttggcgcgac gaacatcacc gcgacgcttg acttcaaccg
     5281 aagcaaccag cggggaatgt gtctggaaag tacgctcgac gccttcgcca gaagagattt
     5341 tgcgaacggt gaaagcagag ttcagaccgc ggttacgctt ggcgataacg acgccttcgt
     5401 aagcctgcag acgctcgcgc gtgccttcct tgacctttac ctgaacgaca atggtgtcgc
     5461 ccggtgcaaa atcgggaagc gtcttgccca aacgggcaat ttcttcttgt tctagttgtt
     5521 caatcaggtt catgtgagat ccttaaaatt ttataaacac ttactcaccg cattgctcct
     5581 ctccgacgat ttccgtgagg agttgcgttt cctccgcgct taacacgcgg tgcgccagta
     5641 aatccggccg gcgcttccgg gttcgaacca gcgactgctt cagccgccag cgacgaattc
     5701 tcttgtggtc gccggataac aacacatccg gcactctctg cccggcatat tcctcgggcc
     5761 gagtatagtg cggacaatcc agcagacccg tcacaaacga atcttctact gccgaagcag
     5821 catctccaag cacccccggc aactggcgga caacggcatc aatcaaggcc attgccggca
     5881 actcgccacc ggaaagcaca aaatccccga ttgaaatttc ctcatcgaca cagcgctcaa
     5941 tcaaacgctc gtcaatccct tcatagcggc cgcaaagcag gattaaacct tcttcacccg
     6001 tcgccagttg catcaccctt tcatgggtga gcggccggcc ctgtggcgag agataaataa
     6061 ctctgctctt ggataacccg gccaaatgct gctgcgcttt tgctgcttca atcgtcctct
     6121 ccagcggccc aggctgcatt agcattccag gaccaccacc aaagggccga tcatcgactc
     6181 gacgccaggc attctcggcg aaatctcgag gattccagcc ttgccaaacc caacgctgtt
     6241 cttccagcgc ccgacgagta attccgcttt gcgtgacggc gacaaacatt tccggaaaaa
     6301 tggtaacgca atcgaagcga atcaccagtc gctgccccac tccacacgaa tgagtcgcgc
     6361 ttctttatct accgttaaca ccactgccgc aacaaaaggc agcagtctct cggtattgtc
     6421 atcggacaca acccttaaaa ccgaactggc gcctgtttcg atcaaactgg ccaccttgcc
     6481 caagcgttct tcagcattgt tgatgacgtc cagcccgatc agatcggacc agtaaaactc
     6541 gccttcgtcg gtcgtcggca atgcttctcg cggcgcacca accaagatac ctttcatggc
     6601 ctctgctgcc gtccggtcta caaccccgtc aagcaacaca accagaccgt tagcgtgaga
     6661 tttcaagcgt ttcagcttgc attcacgcca cgactcacca tcgttactga tccaccagac
     6721 aggcatctca gcccatgcca gcggatcatc accaaaagca tgcaacctga gccagccttg
     6781 cattccgtaa gggtcggcca accgacccag tacgacaata tcgttgccag ccaaagcggg
     6841 aactacttaa gcaacctgct gggcagcgtg ttgcttgacc aggcgggcga cagtcggcga
     6901 caactgggcg ccttgacctt gccaataggc aagacgatcg gttgcaacac gcaggctttc
     6961 tgcattaccg gaggctaccg ggttgtaaaa gccgatacgc tcaacgaaac gaccatcacg
     7021 acgattacgt gagtcggtaa caaccatgtt gtagaagggg cgcttcttgg cgccaccacg
     7081 ggcaagacgg ataacaacca tgggaactct ttcgttagct ggaaaaaaga cccgagatta
     7141 tagcgctttt tcaaggaaaa acaagccact tagaacc
//
LOCUS       DAORPM010000114         7071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2736_length_7071_cov_10.071266, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000114 DAORPM010000000
VERSION     DAORPM010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_7071_cov_10.071266"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLD53_06890"
     CDS             complement(<1..577)
                     /locus_tag="PLD53_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRC60675.1"
                     /translation="MSEKRSGRFESWARYTAGLLMLLVVAVAPCAEMESRAGHANGGR
                     TLTVVMDDNYPPYVFRDSSGMLDGYLVDLWKLWESKTGVRVDLVATDWQKAQQLMADG
                     QADVIDTMFMTQERQRTLDFTPPYELIPVTIFSHSGLGGITGPDTLRGFAVGVKAGDA
                     CISKLRDLGITHLQEFGNYEALVAAAVAGQIR"
     gene            complement(1132..1746)
                     /locus_tag="PLD53_06895"
     CDS             complement(1132..1746)
                     /locus_tag="PLD53_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRC60676.1"
                     /translation="MVATSAMAGIEELVNRRLDELLPQKIEEALRQREEAKTPSMAII
                     ASRGTLDWAYPPFILGSTAAALGWDVSIFFTFYGLNLLKKDISDLKVSPLGNPGMPMK
                     MPFGPDWFKNINWNIPNLIQAGIPGFETVATLLMQQTIRNNGVASIEELRSLALEANV
                     KLIACQMTVDLFGFGHDDFIPGLDYAGAATFLPVAQKADVALFM"
     gene            complement(1857..4043)
                     /gene="glgB"
                     /locus_tag="PLD53_06900"
     CDS             complement(1857..4043)
                     /gene="glgB"
                     /locus_tag="PLD53_06900"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRC60677.1"
                     /translation="MNQSHIDSERQRISEARHHDPFAILGRHPEGKKVIVRAFMPFAA
                     EVRIAEGGMTMTRLPDTDHFEWQGDGKALPEHYSLIWTDSHNREHIGRDPYTFMPQIG
                     DLDLYLFSEGRHRHAHNFLGAHEHEVDGVGGVLFAIWAPNAERASVIGDFNGWDGRRH
                     QMRVRGGSGVWELFIPDLDAGQIYKYEIRNRHTGQVLQKTDPYGQRHEYRPRTGAIVP
                     ALGNYGWGDGDWMERRRSHDWQHAPMSVYEVHLGSWQRGQEGEFLNYREAAHRLVDYV
                     KYMGFTHIELMPITEHPYDPSWGYQTTGYFAATSRFGDPDDCRYFVDYCHRNGIGVLL
                     DWVPAHFPKDAHGLASFDGTPLYEHADPRLGEHKDWSTLIFNFSRNEVKCFLLSSAVY
                     WLEDMHIDGLRVDAVASMLYLDYSRNEGEWIPNQYGGRENIAAIDFLRELNVAVHDQC
                     PGVLMIAEESTSWPQVSRPTYIGGLGFDIKWNMGWMNDTLDYMALEPIYRQYNQGILT
                     FSMLYCFTENFMLPFSHDEVVHGKQSMLHKMPGDEWQRHANLRTLYAYQFTHPGKKLM
                     FMGTEFAQGKEWDSCGVLDWYVLEYPLHQGMQRLVRDLNHLYQASPALHRHEFDWNGF
                     EWIDCHDAQQSVISYLRKGVGDEMMVVVVNLTPVPRYEYRIGVPSPGRYREVLNSDSE
                     IYGGSNVGNGSAPLQADDWPWMNRSHSLLLTLPPLSGVVLAYEGDR"
     gene            complement(4472..4699)
                     /locus_tag="PLD53_06905"
     CDS             complement(4472..4699)
                     /locus_tag="PLD53_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRC60678.1"
                     /translation="MNFDKEVDARGLGCPLPILRSKKALAELQSGQVLHVISTDPGSV
                     KDFAAFARQTGNELLASAENNKEYEFFIKRK"
     gene            4918..5571
                     /locus_tag="PLD53_06910"
     CDS             4918..5571
                     /locus_tag="PLD53_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC60679.1"
                     /translation="MLEIENLCRRFNGRDVLHDVSLRLVPGEYVAIVGESGVGKSTLL
                     NLIAGLDRPDSGRLALDGNSYDGFDDDALTRLRRDKLGFVFQAFHVLPHLTVRQNVAL
                     PLWLQGLDGPAADGPAQQLLDAVGLGDRAASWPRELSGGEMQRVAIARALVHRPRLVL
                     ADEPTGNLDPLNAAKVLSLLAGRIREAGAIGILVTHSLEAAGTADRVLHLRADGLHS"
     gene            5568..>6399
                     /locus_tag="PLD53_06915"
     CDS             5568..>6399
                     /locus_tag="PLD53_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60680.1"
                     /translation="MKLAPVFLGSLSRRWMATLFSFVAIVLGVALGMAVQAVHEAALT
                     EFGRGLRTLAGDADLQVMGPRGGFDEHLYALLAARPEVAAASPVVEVQAKIIGREEPL
                     HLLGLDVFALARVSPALLPRSVEIARSGEQGRAARDRFATLADDKLFLSAAAQTDLGV
                     NVGETIRLQAGAHILDFQIAGDLPGVAENDRIAMLDIAAVQNRFAMLGRLSRIDLRLN
                     AGILPETARTDLQAILPAGVMIEPPQAAENQAANLSRAYRVNLTLLAAMALLTGGFLV
                     F"
     assembly_gap    6400..6409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6410..>7071
                     /locus_tag="PLD53_06920"
     CDS             <6410..>7071
                     /locus_tag="PLD53_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60681.1"
                     /translation="RRRTEFAFLRAIGLARRSLFAWLLAEGALVGLAGGVVGAALGHG
                     LAWLMVATLGGDLGAGYFAGLKPQLQFQPAVTAIYIVLGVVAGVAGAWLPAREAATVS
                     PAQALKAGDEAALPGGRGYPTIGLALLAASLAACWIPPADGLPVGGYLAVVFLLAGSV
                     MLLPVVAAVIAGLISERGSVPSRLAAARLEAAPGHAVVAAAGVLTSVALAAAMAIMVA
                     SF"
BASE COUNT         1357 a         2085 c         2122 g         1497 t
ORIGIN      
        1 cacggatctg ccctgcaacg gcagccgcca ccagcgcttc atagttgccg aattcctgca
       61 aatgcgtaat gcccaaatct ctgagcttgc tgatgcaggc atcgccggct ttgacgccga
      121 ccgcaaagcc acgcagagta tcggggccgg taattccgcc gagcccagaa tggctgaaga
      181 tcgtcaccgg aatcagttcg tagggcggcg tgaaatccag cgtcctttga cgctcttgag
      241 tcataaacat ggtatcgatg acatcggcct gaccgtcagc catcaattgc tgcgcctttt
      301 gccagtcggt ggcgaccaga tcgacgcgta ctccggtctt gctctcccag agtttccaga
      361 gatcgacaag gtatccgtcc agcatgccgc tcgaatcgcg aaatacataa ggcgggtagt
      421 tgtcgtccat caccaccgtt agtgtgcgac cgccattggc atgccctgcc ctggactcca
      481 tttcggcaca gggcgccact gcgactacca gcagcatgag caaaccggcc gtgtatctcg
      541 cccaggactc aaacctgccg ctccgttttt cgctcatccg caagacctcc aagggcaggc
      601 cagcattgct tcggagccat tatggtccgg aacagaaatg gcatcaaggt gacacggccg
      661 acgaggcgaa acgtcagagc ggggtgccgt tgaagcgggc gccgactccg gcgcagagaa
      721 ccggaagcga attcgccaac ctttacaagt cacgttaatg cgctttattc gaatcaagcc
      781 accgagaacg atatctcgcg tcaattttgc gttcgcgctt tcagcaacac gtttgttgac
      841 agaaatcacg tccaacaacc ggcgagtggc cgaatcggcg accacggcaa cgatccagcc
      901 cagcgcagga attgcaatag cacccaatac ttgacggata tcgccttcga cggaagttta
      961 tcgtgtcgtt gcagaagcgt tgaagcccat atcgtggtcc tacaatgata caaaacagac
     1021 gaagatgacg tcgttgtgac gacgcaacat gaaaaacgtt ccctgaattg ttcccaagcc
     1081 ggtttcggaa cgccaactga ccaatcagaa atgccagccg tttcggcagg attacatgaa
     1141 cagggctacg tcggcttttt gcgccaccgg cagaaaggtc gccgcgccgg cgtagtccaa
     1201 acctggaatg aagtcgtcgt ggccaaagcc gaaaaggtcc acggtcatct ggcaagcgat
     1261 gagcttgaca ttggcctcca gcgccaggct acgcagttct tcaatgctgg caacgccgtt
     1321 gttcctgatg gtttgttgca tcagcagcgt ggctacggtc tcgaaacccg gaatgccggc
     1381 ctggatcaag ttgggaatat tccaattgat gttcttgaac cagtccgggc cgaagggcat
     1441 cttcatcggc attccgggat tgccgagcgg actcaccttg aggtcggaga tgtccttctt
     1501 cagcagattc aagccataga acgtgaagaa gatcgaaaca tcccacccca gcgcagcagc
     1561 cgtggagcct aggatgaagg gtgggtaggc ccaatccagc gtgccgcggg acgcaatgat
     1621 ggccattgaa ggcgttttcg cttcttcgcg ctgacgcagt gcctcttcga tcttctgcgg
     1681 cagcaactcg tccaagcggc gattgacgag ttcttcgata ccggccattg cggaagtggc
     1741 aaccatatcg tctcctgatc agccgagtcg ccttgtagat gtttacaggc tatccgggct
     1801 tgatgaaaaa aacatactcc tggctacctt ccgcactggc cggcagttgc atcgatttag
     1861 cgatctccct cgtaggccag aacaactccc gagaggggtg gaagcgtcag cagcagtgaa
     1921 tgcgaacggt tcatccatgg ccaatcgtcg gcttgaagcg gcgcacttcc attgccgacg
     1981 ttgcttccgc cgtaaatttc cgagtcggag ttcaatactt cgcgatagcg cccgggcgag
     2041 ggcacgccaa tccgatattc gtaacgcggc acgggtgtga ggttgacgac cactaccatc
     2101 atttcgtcgc caacgccctt gcgcagatag ctgatcaccg actgctgggc gtcatggcaa
     2161 tcgatccact cgaatccatt ccagtcgaat tcgtggcggt gcagtgccgg gcttgcctga
     2221 tagagatggt tcagatcccg caccaggcgt tgcatgcctt gatgcagggg gtattcgagc
     2281 acgtaccagt cgagtacccc gcagctgtcc cactccttcc cttgagcgaa ctctgtgccc
     2341 atgaacatga gcttcttgcc aggatgcgtg aactggtagg cgtagagcgt acgcagattg
     2401 gcatgccttt gccactcgtc tccgggcatc ttgtgcagca tcgactgctt gccgtgtacc
     2461 acctcgtcat gcgagaaggg cagcatgaag ttctcggtga agcaatagag catgctgaag
     2521 gtgagaatgc cttggttgta ttggcggtat atgggctcga gtgccatgta gtccagagtg
     2581 tcgttcatcc agcccatgtt ccacttgatg tcgaaaccca gaccaccgat gtaggtcggg
     2641 cgactgacct gtggccacga agtcgactcc tcggcaatca tcaggacacc ggggcactgg
     2701 tcgtgtaccg cgacgttgag ttcgcgcagg aaatcgatcg ccgcaatgtt ttcgcgcccg
     2761 ccgtactggt tgggaatcca ctcgccttcg ttgcgcgagt aatcgagata gagcatcgag
     2821 gccacggcgt caacgcgcag gccgtcaatg tgcatgtctt ccagccagta aaccgcactc
     2881 gacaggagaa agcacttcac ttcgttgcgg ctgaaattaa agatcagcgt ggaccagtcc
     2941 ttgtgttcgc caaggcgggg gtcagcgtgc tcgtaaagcg gcgtgccgtc gaagctggca
     3001 aggccgtggg catccttggg gaaatgcgcg ggaacccagt cgagcagtac gccgatgcca
     3061 ttccggtggc agtagtcgac aaagtaacgg cagtcgtcgg ggtcgccaaa acggctggtt
     3121 gccgcgaagt agccggtggt ttggtaaccc caggaggggt cgtaggggtg ctcggtgatc
     3181 ggcatcagtt cgatatgcgt aaagcccatg tacttcacgt aatccacaag tcggtgtgcc
     3241 gcctcgcgat agttgaggaa ctcaccctcc tggccgcgtt gccaggaacc cagatggact
     3301 tcgtacacgg acatcggagc atgctgccaa tcgtgggagc gacgccgctc catccagtcg
     3361 ccgtctcccc agccatagtt gccgagtgcg ggaacgatgg cgccggtgcg cggacgatac
     3421 tcgtggcgtt gaccgtaagg atcggtcttt tgcagaacct ggccggtgtg gcgattacgg
     3481 atttcgtatt tgtagatctg tccggcatcg agatcgggga taaacaactc ccagacgccg
     3541 ctaccaccac gcacgcgcat ctggtggcga cgcccgtccc acccattgaa gtcgccaatg
     3601 acactggcgc gctcggcgtt tggcgcccag atggcaaaca gcacgccgcc gacgccatct
     3661 acttcgtgct catgggcgcc gaggaaattg tgggcgtgcc ggtgacgccc ctcgctgaac
     3721 agatacagat cgagatcgcc gatctgcggc ataaatgtat agggatcgcg cccgatatgc
     3781 tcgcgattgt gactatcggt ccagatcagg ctgtaatgtt ccggcagtgc tttgccgtcg
     3841 ccttgccact cgaaatggtc tgtgtccggc agccgggtca tcgtcattcc gccttcggcg
     3901 atgcgcacct cggcggcgaa gggcatgaat gcacggacga tgaccttctt gccttcagga
     3961 tgccgcccaa ggattgcgaa gggatcatgg tggcgtgctt cgctgatgcg ttgccgttcc
     4021 gaatcgatgt ggctctggtt catagtcaat ctccaagaag tcgtgagtga aggcgtgaaa
     4081 gattctatat actaatattc caattgcgta aaacgtgggc aaacaaattc ttcgttgggc
     4141 gttcaacgtc cgggaaatct tctggagcgg gtattgggtc gacaaaagac gaggcagtgc
     4201 gcgatacaag tcgaggcgtt ggattcaagg cccaactttt tcggtatgaa ctgttggaag
     4261 tgcccattca tgtttttgtc gacaccgtaa ttccacgaag gcaggcaacg cttgactcgc
     4321 gtcaccgcat gagtggtctg cgcttcgtta ttacccactt tcagcgtacg tttattattg
     4381 taccacccca tacacagcat cgcagacaga ctgcgtcatt caggccgcgg tcgcctgctc
     4441 atgtgcgcat ggcggagccg gtaaacacag gctatttgcg cttgatgaag aactcgtatt
     4501 ccttgttgtt ttccgcactg gccagcagtt cgtttccggt ttgcctggca aatgcggcaa
     4561 aatcctttac cgatcccgga tcggtggaaa tcacgtgaag cacctggccc gactgaagtt
     4621 cggccagagc cttttttgag cgcaggatgg gcagagggca gccgaggccg cgggcgtcga
     4681 cttccttgtc gaagttcatg ttcattcctc tctgttgagt ctgcagcggc acgacggatc
     4741 gggtgcttca atgaaccaga atatacatca agcccattcg tcattcacat ccaaatgggg
     4801 cggcgtccga gcctcccgtt aaacgccaag caatccgttt ggcaggaaat acgggcacgc
     4861 caggagtatt ctggagtatt ggcggaaccg accgcaagct gacacctcct agcatctatg
     4921 ctcgaaatcg aaaatctttg ccgccgcttc aacggccgcg acgtgctaca cgacgtctcc
     4981 ttgcgcctcg taccgggcga gtacgtcgcc atcgtcggcg aatcaggtgt tggcaaatcc
     5041 acgctgctca acctcattgc cggtctggac cgccccgaca gcggtcggtt ggcgctggac
     5101 gggaacagct acgacggatt tgacgatgac gcgctcaccc gcttgcgccg ggacaagctg
     5161 ggcttcgttt tccaggcctt ccatgtgctg ccgcatctca ccgtgcgcca gaacgtcgca
     5221 ttacccctgt ggttgcaggg tttggacggt ccggccgccg acggcccggc gcagcaactc
     5281 cttgatgccg tgggtctggg cgatcgcgca gcaagctggc cgcgcgagct ttccggcggt
     5341 gaaatgcagc gcgtggcgat tgcccgcgcg ctggttcatc gcccacgcct ggtgctggcc
     5401 gacgaaccga ctggaaatct tgatccgctc aatgccgcca aggtgctgtc cttgctcgcc
     5461 ggacgtattc gcgaggcagg cgccatcggt atcctggtca cccattcgct ggaggctgcg
     5521 ggcactgcag atcgcgtcct gcaccttcgc gctgacgggc tgcactcatg aaactggcac
     5581 cggtatttct cggctccctg tccaggcgct ggatggcaac cctgttctca ttcgttgcca
     5641 tcgttcttgg cgtcgcgctg ggcatggcgg tacaggcggt tcatgaggcg gcgctcaccg
     5701 agttcggccg cggtctgcgc actctggccg gcgacgctga cctccaggtg atggggccgc
     5761 gcggcggctt tgacgagcat ctgtatgctc tgcttgccgc ccgtcccgag gtggcggccg
     5821 ccagcccagt cgtcgaggtt caggcgaaaa tcatcggccg ggaggagccc ttgcatctgc
     5881 tgggcctgga cgtctttgcg ctggcccgcg tctcgccggc cctgttgccg cgcagcgtgg
     5941 aaattgccag gagtggagag caggggaggg ctgcgcggga tcgctttgcc acgcttgctg
     6001 acgacaagct ttttctcagc gcagcggcac agaccgacct cggcgtgaat gtcggtgaaa
     6061 caatccggct gcaagcgggc gctcacatcc tcgactttca gatagccgga gacctgcccg
     6121 gtgtggctga aaacgaccgt atcgccatgc tggacatcgc cgccgttcaa aaccggttcg
     6181 caatgctcgg gcggctttcg cgcatcgatc tgcgcttgaa tgccggtatc ctccccgaga
     6241 cggcgcgcac cgatctgcag gccattctgc ccgccggggt gatgatcgag ccaccgcaag
     6301 cggccgagaa ccaggccgcc aacctttcac gcgcctaccg ggtcaatctg accctgctgg
     6361 cggcgatggc gctgctcacc ggcggcttcc tcgtgttttn nnnnnnnnnc cgccgacgca
     6421 ccgagtttgc cttcctgcgc gccatcgggc tcgcccgccg cagcctgttc gcctggctgc
     6481 ttgccgaggg cgcgctggtt ggcttggccg ggggcgtggt cggtgctgct ctcggtcacg
     6541 gcttggcctg gctgatggtc gccaccctcg gcggtgatct gggagcaggc tattttgccg
     6601 ggctgaagcc gcagttgcag ttccagcctg cagtcacggc catttacatc gtgctgggcg
     6661 tagtcgccgg cgtagcgggt gcctggctgc cggcgcgcga ggccgctacg gtgtctccgg
     6721 cacaggcatt gaaagccggc gacgaagcag ccttgcccgg tgggcgcggg tatccgacta
     6781 tcggcttggc ccttcttgcg gccagtcttg ccgcgtgctg gattccgcct gcggacggac
     6841 tgccggtggg tggctatctg gcggtcgttt tcctgctggc gggaagtgtg atgcttttgc
     6901 cggtggtggc tgccgtaatc gccgggttga tttcggagcg gggttcggtg ccctcccgcc
     6961 ttgccgccgc acggctggaa gcggcgccgg gacacgcggt ggttgcggca gccggagtgc
     7021 tgaccagcgt cgcgctggcg gcggcgatgg cgatcatggt cgcctcgttc c
//
LOCUS       DAORPM010000115         7014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2769_length_7014_cov_11.330938, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000115 DAORPM010000000
VERSION     DAORPM010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2769_length_7014_cov_11.330938"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..953)
                     /locus_tag="PLD53_06925"
     CDS             complement(186..953)
                     /locus_tag="PLD53_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRC60682.1"
                     /translation="MSIDYSVLAVLSCAALFAGFIDSIVGGGGLIQIPALFTALPKEL
                     PATLFGTNKIASVFGTANAAWHYSCRVRMPWRTTLPAAVAAFGFSFLGAMAVAWLPRD
                     LLRPMILALLVVSAAYIFWRKDFGSIHRPQHAGRRELAYALIVGGAIGFYDGFFGPGA
                     GSFLIVLFIRFFGFDFLHASAASKVVNVATNLAAISFFVPNGFYLPLAALCMAVLNVL
                     GSVIGSQLALRRGSGFVRLVFLAVVSALILKFAWDTF"
     gene            complement(957..2333)
                     /gene="mnmE"
                     /locus_tag="PLD53_06930"
     CDS             complement(957..2333)
                     /gene="mnmE"
                     /locus_tag="PLD53_06930"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRC60683.1"
                     /translation="MAEKPPTTPRPDSGPYPETIAAIATATGRGGIGVIRISGGGLLD
                     FARALSGKQLRARQATLVDFRAADGSSIDSGLLIYFPAPCSFTGEDVLELQGHGGAVV
                     MQMLLARCLDLGARLAEPGEFTRRAYHNGKLDLAQAEAVIDLIDAATTTAARSAVRSL
                     QGEFSNEVNVLLEQLIELRALVEATLDFPEEEVDFLEAADAYGRLERLQQSLARVFDR
                     AHQGRLLQSGLHVVLAGQPNVGKSSLLNRLAGDDLAIVTSVAGTTRDVVRSTLQIEGI
                     PLHIIDTAGLHATEDEIEKIGMERTWREIERADVVVLLADARSGIGTAERVILDQLPA
                     HLRRITVFNKIDLAGHQAECHDEAGGLAISLSAKYGEGIELLRQQLLKIAGWHPAEDV
                     FIARERHLRALADTRKHIETALARLPELELFAEELRLAQAALASITGKFGADDLLGEI
                     FGRFCIGK"
     gene            complement(2299..3996)
                     /gene="yidC"
                     /locus_tag="PLD53_06935"
     CDS             complement(2299..3996)
                     /gene="yidC"
                     /locus_tag="PLD53_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRC60684.1"
                     /translation="MDNRRLILLLVFSFSLVMLWDAWQKYNQPKVAAQIAAVTVAADG
                     SPVPVPSATQASPASPVSVPVVSAKSAVAVGEIVSVNTDLFTADISTQGGDIVGLELN
                     NYKAIDDKKKKFSLFSAKHQYAAQSGLIGENLPNHKTKFTVVDGNRELAPDAKTVKLR
                     LEAPAAGGVKVAKVYTFTRGSYLINVDYEIDNGSNKELSTHAYYQLQRDTVAPAGENM
                     MVSTFTGPAVYTEQDKYQKIPFPDIEKGKAKFNAMADNGWIAMVQHYFVSAWIPQEKL
                     SREFYVRNLEGGVNPAVTAGVIVPIPVVAPGAKITQSTSLFAGPQIQSRLEQLAKPVA
                     DGGIDAQGLPLVVDYGWLTIVAAPIFWCLDFIHKIVGNWGWAIVLLTIGIKAIFFPLS
                     AASYKSMAKMKAVTPRLTALRDRYGDDKQRLNQEMMNLYKTEKINPLGGCLPILVQIP
                     VFIALYWALLGAVEMRDAPWILWITDLSTQDPYYVLPVIMVLTMMIQTKLNPKPPDPI
                     QAKITAYMPFIFGAMFFFFPAGLVLYWVVNNVLSIAQQWQITRMIERGGKAANDAKA"
     gene            complement(4020..4229)
                     /gene="yidD"
                     /locus_tag="PLD53_06940"
     CDS             complement(4020..4229)
                     /gene="yidD"
                     /locus_tag="PLD53_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRC60685.1"
                     /translation="MKLLLLALIRIYKYAISPFLGRRCRFFPSCSEYAAEAVEKHGAF
                     RGTRLVLARISRCHPWNPGGFDPVP"
     gene            complement(4234..4629)
                     /gene="rnpA"
                     /locus_tag="PLD53_06945"
     CDS             complement(4234..4629)
                     /gene="rnpA"
                     /locus_tag="PLD53_06945"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRC60686.1"
                     /translation="MNASKLAGEHQVSNFAFPRDQRLIRTDDYSSVFGFRKAIRSTHF
                     LLHYRTRVAEEVPGARLGLVVAKRYLKRSVDRNLVRRLARENFRLMHCRLRSRDLILR
                     LAAKPEPLDRRVLNEEIRGLLGKMISPER"
     gene            complement(4626..4760)
                     /gene="rpmH"
                     /locus_tag="PLD53_06950"
     CDS             complement(4626..4760)
                     /gene="rpmH"
                     /locus_tag="PLD53_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRC60687.1"
                     /translation="MKRTYQPSVTRRKRTHGFLVRMATRSGRAVIRARRAKGRHRLAV
                     "
     gene            5006..6409
                     /gene="dnaA"
                     /locus_tag="PLD53_06955"
     CDS             5006..6409
                     /gene="dnaA"
                     /locus_tag="PLD53_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRC60688.1"
                     /translation="MSSFWIACLSQFEQELPPQQFNTWIRPLRLEGEDDFANGMRLLA
                     PNGFILKWVKERYLTRIEELGSAFFSTPVRVSLILGERTTVNHEPSPSSSTQQVAPTE
                     NPDKLKSSPLAPQIRSLEKKSSHYETTRLISGFTFDNLIVGKANDLARAASAQVSINP
                     GGAAYNPLFIYGGAGLGKTHLIHAIGNRILEQNHVKVVRYVHAEDYYSDVVRAYQTKS
                     FDAFKRYYRSLDVLLLDDVQFFNGKNRTQEEFFFVFNALIEAKKQIVISCDTYPKDIS
                     GLEDRLITRFDWGLTVQIEPPEIEMRVAILKKKAEVENVDLDDEVAFYIAKHLRSNVR
                     ELEGALKKVLAYASFHGLKIDLDLAKTSLKDLIGAVNRQITVENIQKTVADYFKIKVS
                     DLFSKKRTRQIVRPRQVAMWLAKNLTSQSYPSIGEAFGGRDHTTVLHAVRTIEMLRGK
                     DNELNHDVHLLLQVLKG"
     gene            6620..>7014
                     /locus_tag="PLD53_06960"
     CDS             6620..>7014
                     /locus_tag="PLD53_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRC60689.1"
                     /translation="MLFIKTQRDALLTPLQSVSGIVEKRHTLPILSNVLLEKKADILT
                     LVATDIEIQITTSTGGAVGDGDGAVTVGARKLQDILRSLPDSSEVSLNLEDKRLQVRA
                     GKSRFVLQTLPADDFPRMALSDGEQKKIST"
BASE COUNT         1744 a         1832 c         1849 g         1589 t
ORIGIN      
        1 aaaatgccgc ctccgcatgc tttcgatgac gcggcgtggg tcggttacag gctaagcgaa
       61 gctgttccag tgcaggcgct cgccaagcaa aagctgttgg aacttgaaga cccggtatcc
      121 cggctcgaaa ttctccacac ctaccttgcg caacgcaatc tggttgcata ggggccgaaa
      181 caggatcaga aggtatccca cgcgaatttg agtataagcg cactgacgac cgccagaaat
      241 accagccgga cgaatccgct tccgcgccga agggcaagct gcgagccgat cactgaaccg
      301 agtacattga gaaccgccat gcacagcgca gccaggggaa ggtaaaaacc gtttggaaca
      361 aagaaactga tcgcggcgag gttggtcgcg acgttgacca ccttcgatgc ggccgaggca
      421 tgcaggaaat caaaaccgaa gaagcggatg aaaagaacga tcaggaaact ccccgccccg
      481 ggtccgaaaa acccatcgta aaagccgatc gctccaccga caatcagcgc gtaggccagt
      541 tcgcggcgac cggcgtgttg tggtcggtga atggagccga aatccttgcg ccagaagata
      601 taggctgctg aaacgaccag caacgcaagg atcatcgggc gcagcaaatc ccgcggcagc
      661 caggctaccg ccatcgcccc cagaaaggaa aatccgaagg cggcaacggc ggccggcagc
      721 gtggttcgcc agggcattct taccctgcag gaatagtgcc aggcggcgtt cgcagtaccg
      781 aaaacactgg caattttgtt cgtgccgaag agggtggccg ggagctcctt cggcaacgcc
      841 gtgaacagtg cgggaatctg aatcagccct ccaccgccaa cgatagaatc gataaacccg
      901 gcaaacaaag cggcacacga gagtacggca agcacggaat aatcgatact cacgatctac
      961 tttccgatac aaaaccggcc aaatatttca cccagcagat cgtcggcgcc aaacttgccg
     1021 gttatcgaag caagtgccgc ctgagccagc cgcaattcct cggcaaagag ttcgagttct
     1081 ggcaaccggg ccaacgcagt ttcgatgtgc ttccgggtgt ctgccagtgc gcgcaaatgg
     1141 cgttcgcggg caatgaacac atcctccgcc ggatgccaac cggcaatctt cagcaactgc
     1201 tggcgcagca gttcaatccc ttctccgtac ttggcagaaa gcgatatggc caagccgccg
     1261 gcctcatcat ggcattcagc ctgatgtccg gccaggtcga tcttgttgaa aacggtgatg
     1321 cgccgaagat gcgcaggaag ctggtcaaga atgactcttt cggcagttcc gataccgctg
     1381 cgggcgtctg ccagcaacac caccacatcc gcgcgctcga tctcgcgcca tgtccgttcc
     1441 atgccgattt tttcgatttc gtcttcggtg gcgtgcaggc cggcagtatc aatgatatgc
     1501 agtggaatgc cttcgatttg cagcgtgctg cgcacgacat cgcgcgtggt gcctgccacc
     1561 gatgtaacaa tcgccagatc gtcaccggca agacgattga gaaggctgga ctttccgaca
     1621 ttgggctggc cggcgagcac gacatgcagg ccactttgca ggagccggcc ctggtgagcg
     1681 cggtcgaaaa ccctggccag actctgctgc aagcgctcaa ggcggccata ggcgtcagcc
     1741 gcttcgagaa aatcgacttc ttcttcggga aaatcaagcg ttgcctcaac caatgctcgc
     1801 agttcgatca attgctcaag cagcacattg acctcgttcg agaattcgcc ctgcagcgaa
     1861 cgaacagcac tgcgcgcggc cgttgtagtg gccgcatcga tcagatcgat taccgcttca
     1921 gcttgagcca ggtcgagctt tccgttgtga taggcacggc gggtaaattc gcctggctcg
     1981 gccaatcgag cacctagatc aaggcaccgg gcgagcagca tctgcattac gacggcaccg
     2041 ccatgtccct gcaattcgag aacatcctcg ccggtaaatg agcacggggc ggggaagtag
     2101 ataagcagtc cgctgtcgat tgagctaccg tcagccgcac gaaaatcgac cagcgttgcc
     2161 tggcgggccc gtagctgttt accactcagt gcgcgggcaa agtccagcag gccgccgccg
     2221 gaaatgcgga taacgccgat gccgccacgg ccggtagccg tagcgatagc tgcgatggtc
     2281 tccgggtacg ggccggaatc aggccttggc gtcgttggcg gcttttccgc cacgttcgat
     2341 catccgggtg atctgccatt gctgggcaat tgaaaggacg ttattgacca cccagtacag
     2401 caccaaaccg gcagggaaga agaagaacat ggcaccgaaa atgaatggca tatacgccgt
     2461 gattttggcc tgaatgggat cgggcggctt cggattgagc ttggtctgta tcatcatggt
     2521 gagtaccatg atcaccggca gaacgtagta cggatcctgc gtggacaaat ccgtaatcca
     2581 cagaatccaa ggcgcatccc gcatttcgac ggcacccagt agcgcccagt aaagtgcgat
     2641 aaagacagga atctgcacca gaatcggcaa gcaaccgcca agcggattga tcttctctgt
     2701 cttgtagaga ttcatcatct cttggttcag acgctgtttg tcgtcaccgt aacggtccct
     2761 caatgctgtc aaacgagggg tgacagcctt catcttggcc atcgatttgt agctggccgc
     2821 ggacaaaggg aagaagatcg ccttgatgcc aatcgttaac aagactatgg cccaacccca
     2881 gttgccgaca atcttgtgga tgaaatccag acaccagaaa atcggggcgg cgacaatggt
     2941 cagccagccg tagtcgacaa ccaagggcag gccctgggca tcgataccac catccgccac
     3001 cggcttggca agttgttcga gtctcgactg gatctgtggt ccggcaaaaa gtgaggtcga
     3061 ctgagtgatc ttggcgcctg gcgcgacaac cggaatcggg acaataacgc cggccgtaac
     3121 cgccggattg accccaccct ccagattacg gacataaaac tcgcgtgaca gcttttcctg
     3181 tggaatccac gccgatacaa aatagtgctg caccattgct atccaaccgt tatcggccat
     3241 agcattgaac ttggccttgc ccttttcgat atcggggaag ggtatctttt gatacttgtc
     3301 ctgctccgtg tacacggctg gtccggtaaa ggtgctgacc atcatgtttt cacctgccgg
     3361 cgctaccgta tcgcgctgca attgatagta ggcatgcgtg gataattcct tgttactgcc
     3421 attgtcaatt tcatagtcga cattaatcag gtagctgcca cgggtgaagg tataaacttt
     3481 ggctaccttg acaccgcctg cagcgggcgc ttccagccga agcttgaccg ttttcgcgtc
     3541 gggcgcaagt tcacgatttc catcaacaac ggtgaatttc gttttgtgat ttggtaggtt
     3601 ttcaccgatc aaaccacttt gcgcggcata ctggtgtttc gctgaaaaga gcgagaattt
     3661 cttcttcttg tcatcgatcg ccttgtagtt attcagctcg aggccgacga tatccccacc
     3721 ctgcgttgag atatcggcag taaagagatc cgtattgacg ctaacgattt cgcccactgc
     3781 aaccgctgac ttggcggaaa ccactggcac agaaacgggg cttgccggag aggcttgtgt
     3841 tgcagacggc accgggacag gagaaccatc cgcggcgacg gtaacggcgg ctatctgagc
     3901 cgccaccttg ggctggttgt atttttgcca ggcgtcccag agcatgacca gcgagaacga
     3961 aaaaactaat aaaaggatca ggcgtcggtt atccatgaac gacctatgca attgagactt
     4021 cagggtacgg gatcaaaacc gcccgggttc cacgggtggc aacgggatat gcgtgcaagt
     4081 acaagacgtg tgcctctgaa tgccccgtgt ttttccaccg cttcggctgc atactccgag
     4141 cagctcggaa aaaatcggca acgacggcca agaaaagggc tgattgcgta tttgtagatg
     4201 cgaatcagtg ccagcagcaa caatttcatc ggctcatcgt tcgggtgaga tcattttgcc
     4261 cagcaagccc cggatttctt cgttcaggac tcgccggtcc agtggttccg gctttgccgc
     4321 caagcgtaga atcagatccc gcgaacgtag gcgacaatgc atgaggcgga agttttctcg
     4381 ggccagtcgc cggacaaggt ttctatctac cgaacgtttc agatatcgtt ttgcaacgac
     4441 tagccccaat cgggcaccgg gtacttcttc ggccacccta gtccggtaat gtaacaggaa
     4501 atgagtgctc ctaatcgcct tcctgaaacc aaaaacggat gaatagtcat ccgttcgaat
     4561 aagacgctgg tccctgggga acgcgaaatt tgaaacttga tgttcgcctg ccagtttgga
     4621 ggcgttcaaa cggccaaacg atgacggcct ttagcgcgac gcgcgcgaat cacagcccga
     4681 ccgctacggg tagccatacg gacaagaaag ccgtgggtgc gcttgcggcg tgtaaccgag
     4741 ggttgatagg tacgtttcat gagtagttcc ttgaattcat tacaaagcag gtcggcaatt
     4801 agacattgtc agggttgctt ttgtcaagac caattaggtt caccggtcca acaatatgag
     4861 cgagctgtgg ataactttca gccgaagggt tagaatcatc ccttcctaaa aagctcaaaa
     4921 acctgctgga aaaggaaatc gagtagcgat ttttttctcc aagccagcac ctgaccggaa
     4981 tcctattaga ttgtagattg caaacatgag tagtttctgg atcgcctgcc tgagccagtt
     5041 tgaacaagaa cttccgcctc agcaattcaa tacttggatt cgtccacttc gacttgaagg
     5101 tgaagatgat ttcgccaatg gtatgcgcct gcttgcgccc aacggtttca ttctgaagtg
     5161 ggtaaaggaa cgctacctga ctcgtattga agaactcggg agtgcgttct tctccacacc
     5221 tgtcagggtt tcattgattc tgggcgagcg cacaacagtt aaccatgaac cctcaccgtc
     5281 gtcctcaacg caacaagttg cgccaacaga aaaccccgac aaactgaaaa gtagtccgtt
     5341 ggcccctcag attcggtcac tggaaaagaa gtcttctcat tacgaaacaa ctcgtcttat
     5401 ttccggtttt accttcgata acctgatcgt cggaaaagct aatgacctgg cgcgagccgc
     5461 ttcggcacaa gtctcaatta acccaggggg cgctgcctat aacccgctgt tcatttatgg
     5521 gggagccggc cttgggaaga cccaccttat tcacgcaata ggcaatcgta ttctcgaaca
     5581 gaatcatgta aaagttgttc gttatgtaca tgctgaggat tattactcgg atgtggtgcg
     5641 tgcttatcag accaaatcgt ttgatgcttt caagcgttac tatcgatccc ttgatgtgct
     5701 gttgcttgac gacgtgcagt tcttcaatgg aaagaaccgg actcaggaag aattcttctt
     5761 tgttttcaac gcactaattg aagcaaaaaa gcagattgtc atatcgtgcg atacgtatcc
     5821 gaaggatatt tctggtttgg aagaccgcct gattacccgc ttcgattggg gtctaacggt
     5881 tcagatcgaa cccccggaaa tcgagatgcg cgtcgccatt ctgaagaaga aagcagaagt
     5941 tgaaaacgtt gatctcgacg atgaagtcgc tttttatatt gccaaacacc ttagatccaa
     6001 tgttcgtgaa ctcgaaggcg ccctcaaaaa ggtacttgcc tacgcgtctt tccatggcct
     6061 gaaaatcgat cttgatcttg cgaaaacctc tctgaaagat ctgattggcg cagttaatag
     6121 gcagattacc gttgaaaaca tccagaaaac agtcgcagat tacttcaaga tcaaagtatc
     6181 agacctgttc tcaaagaagc gtacgcgcca gattgttcgc ccgcgacagg tcgccatgtg
     6241 gctcgctaag aatctaacat cgcaaagcta tccgtctatt ggagaggcgt ttggtggtcg
     6301 tgaccataca accgttttac atgctgtgcg cacaattgaa atgcttcgtg ggaaggataa
     6361 cgagctaaac cacgatgttc acttgctgct gcaggtattg aaaggctaat gaaaacatgt
     6421 agaagttcat gcattggctg tgaacaataa gtgcataagg tctgaattac gagtgtgtta
     6481 acaacttgtt cttttttatc cacaagagga tacagagcct ttacagtctg tttttttgct
     6541 ctaagtatat gataattaat gataaattgt ttatatccct aaagttgtct ttgatattat
     6601 ctttatagga tttatatata tgctttttat taaaacccaa cgtgacgcac tgttgacacc
     6661 tttgcagtct gtttccggaa ttgttgagaa aagacatacg ctgccaattc tgtcgaatgt
     6721 gttgcttgag aagaaggcag atatattgac cttggtggct accgatatcg agatccagat
     6781 cactacttcg actggtggcg ccgtcggcga tggtgatggg gcggtaaccg taggcgcgcg
     6841 gaaacttcag gatattcttc gatccttgcc ggatagttca gaagtaagtt tgaaccttga
     6901 ggataagcgt ttgcaggttc gagccggaaa gagccgtttt gttctgcaga ctttgccagc
     6961 cgatgatttc cctcgtatgg cattgtcgga tggtgagcag aagaaaattt cgac
//
LOCUS       DAORPM010000116         6957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2804_length_6957_cov_13.506085, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000116 DAORPM010000000
VERSION     DAORPM010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2804_length_6957_cov_13.506085"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PLD53_06965"
     CDS             complement(<1..1114)
                     /locus_tag="PLD53_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRC60690.1"
                     /translation="MEIFKADVVIVGGGGAGLRAAIAVAETDPSLKIALVSKVYPMRS
                     HTVAAEGGAAGVKKPTDSFEYHFHDTVGGGDWLCEQDVVEYFVKQAPVELTQLEHWGC
                     PWSRTEDGHVNVRAFGGMKIERTWFAADKSGFHILHTLFQTSIKYPSIKRFDEHFCVD
                     LVEEDGRCQGVVAIEMSTGEFSLIEAKSVIIATGGAGRVFRESTLGGIVTGDGMALAY
                     RHGVALRDMEFVQYHPTCMPVTGLLFTEACRGEGGFLVNKDGYRYLQDYGLGPLPDKP
                     KNKYMELGPRDRLSQAFFFEQQKGRTIEHPTLGSVVHLDLRHLGEAYLHERLPLIYEM
                     AETFMGLDPAKEPIPVRPGVHYTMGGIKVDGNCAASL"
     gene            complement(1292..2110)
                     /locus_tag="PLD53_06970"
     CDS             complement(1292..2110)
                     /locus_tag="PLD53_06970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRC60691.1"
                     /translation="MLIDTHCHLDAHEFADDRRALVHAAHQAGVSAMIIPSVEAGNFS
                     AVRDCCTTYAACFPAYGIHPLSLDRASEVDLITLRQWLKTESAGALAPVAVGEIGLDF
                     YMPGFDAARQEHFFIEQLRIAKDLDLPVLLHVRRSVDQVLKCLRRIGSPGGIAHAFNG
                     SRQQAEAFIGLGFKLGFGGAMTYPGSSRIRRLAATLSMDSIVLETDAPDIPPVWLSGE
                     RNTPVELVRIARTLSDLRGISHEEVLKVTSANVLSLLPRVLPSLSDSASPQVAG"
     gene            complement(2144..3391)
                     /locus_tag="PLD53_06975"
     CDS             complement(2144..3391)
                     /locus_tag="PLD53_06975"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HRC60692.1"
                     /translation="MNVLVFGAGVIGVSSAWYLAQAGHKVTVMDRQPGAGMETSFANG
                     GQISVSHAEPWSNPHAPLRALCWMGREDAPLLFRLRWDAALFEWSLRFLSECTAQRTR
                     ANIRDIVTLASYSRNQLKALREETGIQYDHLERGILHVYTDRKELETAVEASKVLREY
                     GCDRRTINAYECVAIEPALETAQPLLVGGQYTAEDESGDAHRFTKQLAELCRLQGVTF
                     RYGTTIDQIEAADGHVTGALVTSSTGRDVVKADAYVVALGSYTPLLLRPLGIRIPVYP
                     AKGYSATVALDDSSIAPSVSMTDDAYKLVFSRLGQRLRIAGTAEFNGYNTELNSSRCN
                     ALMQRTRQLFPELRPAGKPEFWCGLRPSTPSNVPLIGRSALSNLYLNTGHGTLGWTMA
                     CGAGKALADIVGGRSPEIELRRI"
     gene            complement(3395..5524)
                     /locus_tag="PLD53_06980"
     CDS             complement(3395..5524)
                     /locus_tag="PLD53_06980"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRC60693.1"
                     /translation="MYENEDPAYRVFVDTLSYLEPDEIALVVDSYRFSEEAHKGQSRL
                     SGEPYITHPLAVAGALAEWRMDAQAVIAALLHDVMEDTAVTKDEIAVRFGKQVADLVD
                     GLSKLEKIEFQPFQVAQAENFRKMLMAMAHDLRIVMIKLADRLHNLQTMAHIRLEKRR
                     RIARETLEIYVPIATRLGLNNISRQLQDLAFKHVHPMRFRVLKRAIKAVRGNRRELLS
                     KTLDGIYSKLHEDNIEAGVFGREKSLYSIYRKMVEKHLSFSQVLDIYGYRIIVRDIPT
                     CYLALGSLHSRYKPVPGKFKDYIAIPKGNGYQSLHTTLIGPYGTPIEIQIRTQSMHNL
                     AEEGIASHWLYKDSEDSGAHLQVKMHKWLHSLLELQSSTGDSSEFLEHVKVDLFPDEV
                     YVFTPKGKILALPRGSTVIDFAYAVHTDIGHHCVAARIDHELISLSTELANGNQVEII
                     TAPQASPNPVWLTYVKSGRARSKIRQFLRASQQNGSVDLGENMLKQELHVLGVLPSEI
                     SEEVWERVIRDSGKKSKREFFADIGLGRGLATIFAHRLAARGESTQAETVKPTPLLVR
                     GTEGTAVQLASCCLPIPGDPIIGFLRQGQGLQIHTTTCSVALKSRNTEQQNWIHVGWE
                     PDPGQHFDVRIKVTALNVRGVLGRVATGISQAGANIENVSMDGENLGMYTVLHFLVQV
                     SDRLHLATLMRGLRRMTDVVRIVRDQE"
     gene            complement(5625..5831)
                     /gene="rpoZ"
                     /locus_tag="PLD53_06985"
     CDS             complement(5625..5831)
                     /gene="rpoZ"
                     /locus_tag="PLD53_06985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRC60694.1"
                     /translation="MARVTVDDCMQFIPNRFQMTLAATYRARQITAGATPMIDIDRDK
                     PTVIALRELALGMYGLEVLNRGHA"
     gene            complement(5848..6456)
                     /gene="gmk"
                     /locus_tag="PLD53_06990"
     CDS             complement(5848..6456)
                     /gene="gmk"
                     /locus_tag="PLD53_06990"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRC60695.1"
                     /translation="MPGQLFIVAAPSGAGKTTLVSLLIGNDPGIHVSISTTTRTPRPG
                     EENGREYHFVDVQNFREMVHRNEFVEWAEVHGNYYGTSLQWIKMEMDAGRDVLLEIDW
                     QGAQQVRKSFPEAVSVFVMPPSLEALGERLSGRGTDSVETILRRIGAARDEMRHADEF
                     DYVIINDELSRALDDLQSIVNASRLKYTIQCQRHAALFAALL"
     gene            6597..6950
                     /locus_tag="PLD53_06995"
     CDS             6597..6950
                     /locus_tag="PLD53_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60696.1"
                     /translation="MSRRMALLPKMFLVAALATTGAGAVTLKVVGFDDMSCHAWVQSK
                     DDSEQRRVYLAWIRGVLTGHNYARLKQQVSDISENTVENFVDRYCVEKPNGQFSDAAL
                     RMSDRFSGRDEAITR"
BASE COUNT         1553 a         1957 c         1954 g         1493 t
ORIGIN      
        1 tgagcgatgc ggcgcagttg ccgtcgacct tgatgccgcc catggtgtaa tgcactccgg
       61 ggcgcaccgg gatcggttcc ttggcggggt caagccccat gaaagtctcg gccatttcgt
      121 aaatcagtgg caggcgttcg tgcaaatacg cttcgccgag atgacgcagg tcaagatgaa
      181 ccaccgagcc gagagtcgga tgctcgattg ttcggccttt ctgctgttca aaaaagaagg
      241 cctggctgag gcggtcgcgc ggaccgagtt ccatatactt gttctttggc ttgtccggca
      301 acgggccgag accgtaatcc tgcaagtagc ggtagccgtc cttgttgacc agaaagccgc
      361 cttcgccgcg acaggcctcg gtaaagagca agcccgtaac cggcatgcag gtcgggtgat
      421 actgcacgaa ttccatgtcg cgcaaagcga cgccatggcg ataggccagg gccatcccgt
      481 cgccagtgac gatgccgccg agtgtgcttt cgcggaagac gcgccccgct ccgccggtgg
      541 cgataatcac ggacttggct tcaatcaggc tgaattcgcc ggtcgacatc tcgatggcaa
      601 caacaccctg acaacggcca tcttcttcga cgagatcgac gcagaagtgc tcgtcgaagc
      661 gcttgatcga gggatacttg atcgaggtct ggaacagggt atgcagaata tggaatcccg
      721 acttgtcggc cgcgaaccat gtacgctcga tcttcatgcc gccaaaagcg cgcacattga
      781 catggccgtc ttctgtacgg ctccaaggac agccccaatg ttcgagctgg gtcagttcaa
      841 ccggcgcctg cttgacgaag tattcgacga catcctgttc gcacagccag tcgccaccgc
      901 ccacggtgtc gtggaagtga tactcgaaac tatcggttgg tttctttacg cccgccgcac
      961 cgccttcagc ggccaccgtg tggctacgca tcggatacac tttggagaca agcgcgatct
     1021 tcagcgaagg atctgtctct gctacagcga tagctgcgcg caaaccggcg ccgcctcctc
     1081 ctacaattac cacatccgcc ttgaaaattt ccacgcccaa tcctttctcg ctttacgcga
     1141 actcatcgac gacctgcgca aatcaaatct ttgacaaagc gcattgtggc ggcgcttcat
     1201 gcctgacagg ctcggattat ggcgttattt gtacgctctg gcaacttcgg ttcagaactt
     1261 ctctcacgag aaaagaacca tccgaagatc gtcatccagc cacttgcggc gatgcagaat
     1321 ccgacaaact cggcaacacc cgaggcaata gcgaaagcac attggccgac gtgactttca
     1381 gcacttcctc gtgcgatatg ccgcgtaagt ccgacaaggt tcgggcgatc cggaccaact
     1441 caaccggcgt attgcgctcc ccggacagcc agaccggcgg aatatcaggt gcgtcggttt
     1501 caagaacaat tgaatccatc gacagggtgg cagcaagccg acggattcgc gatgaccctg
     1561 ggtaggtcat ggcaccgcca aaacccagct tgaagccgag accaatgaat gcttcagctt
     1621 gctggcggct gccgttaaag gcatgcgcga taccacccgg agaaccaatc cggcgcaggc
     1681 atttcagtac ctgatcgact gaacggcgta cgtgcaggag cacgggcaaa tccagatcct
     1741 tggcaattct cagctgctcg ataaagaaat gttcctgacg cgctgcatca aaacccggca
     1801 tatagaaatc gaggccgatt tcaccaacgg cgactggtgc aagcgctccc gccgactcgg
     1861 ttttcagcca ttgacgcagc gtgattaggt ctacctcact cgctcggtca agggaaagcg
     1921 gatgaatgcc ataggccgga aaacacgccg cgtaagtcgt gcagcaatca cgaacggccg
     1981 agaaattacc tgcttcgaca gaagggatga tcattgcgga gacgccggct tgatgtgcgg
     2041 cgtgaaccag tgcgcgccga tcgtcagcga actcgtgggc gtccagatgg cagtgggtgt
     2101 cgatcagcat atgcaatgta atagtggcgt tgcgagcccg gcctcagatg cgccgcagct
     2161 cgatttcagg tgatcggcca ccgacgatat cggccagtgc cttgccggcg ccacaagcca
     2221 tggtccagcc gagtgtgccg tgtccggtat tgaggtacag attggatagc gccgagcgtc
     2281 caatcaaagg tacgttcgac ggcgtagagg gccgcaggcc gcaccagaac tcaggcttgc
     2341 ccgcaggtcg aagctctgga aacaattggc gtgtgcgctg catcaaggcg ttgcagcgcg
     2401 aactgttgag ttcggtgttg taaccgttga attcggctgt tccggcgata cgaaggcgct
     2461 ggccaaggcg tgaaaagacc agcttataag catcgtcggt catggacacg ctgggcgcga
     2521 tactggaatc gtcgagagcg accgttgccg agtagccctt ggccggatag acgggaatac
     2581 gtattcccaa ggggcgcaac aagagcggcg tgtagctgcc gagtgcgacg acgtaggcgt
     2641 cagccttcac tacatcacga cccgtcgacg aggtcactag agcgcctgtc acgtgcccat
     2701 ccgccgcttc aatctggtct atcgtagtcc catagcggaa tgtgacaccc tgcaatctgc
     2761 acagctccgc gagttgtttt gtaaaacgat gcgcatcgcc cgactcgtct tcagccgtat
     2821 attgaccacc gaccaggagg ggctgcgccg tttccagcgc gggttcgatc gctacgcact
     2881 cgtaagcgtt gatcgttcgc cgatcacaac catactcgcg taataccttg gacgcttcaa
     2941 cggctgtctc aagctctttg cggtcggtat agacatgcag gatgccgcgt tcgagatggt
     3001 catactgaat acccgtttct tcgcgcagtg ctttgagctg gttgcgactg tatgacgcca
     3061 aggtaacgat gtcgcgaata ttggcgcgcg tgcgttgcgc cgtgcattcg ctcaagaaac
     3121 gcaaactcca ttcgaaaagc gctgcgtccc agcgcaaacg aaataagagc ggtgcatcct
     3181 cgcgccccat ccagcagagc gcacgcagtg gagcgtgcgg attagaccag ggttcggcgt
     3241 gcgaaacgga tatctggccg ccattggcaa aactggtttc catgccagcg cccggctggc
     3301 gatccattac cgtgacctta tggccggctt gggccagata ccaggcacta ctgacaccaa
     3361 tgacaccggc gccgaagacc aggacgttca acaactactc ctgatcgcga acgatacgca
     3421 caacgtcagt catacggcgc agtccgcgca tcagtgtggc cagatgcagg cgatctgaaa
     3481 cctgcacaag aaagtgcagc accgtgtaca tgcccaggtt ttccccgtcc atgctgacat
     3541 tctcgatgtt ggcgccagcc tgcgatatgc ctgtcgcgac tcgacccagg acgcctcgga
     3601 cattcaatgc ggttaccttg atgcgaacat caaagtgctg gccgggatca ggttcccacc
     3661 cgacgtgaat ccagttttgc tgctcagtgt tgcgtgattt cagggccaca gagcaggttg
     3721 tagtatgaat ctgcaagcct tgaccctggc gcagaaaacc gataatcgga tctcccggaa
     3781 ttggcaggca gcatgatgcc aactggactg ctgttccttc ggtgcctcgt acgagtagcg
     3841 gcgttggttt aacagtctct gcctgtgtcg attcgccacg cgccgccaga cgatgagcaa
     3901 aaatggttgc cagaccgcgg ccaagcccaa tatcggcaaa aaattcccgt ttcgatttct
     3961 tccccgaatc gcgaatcact cgctcccaga cttcctccga aatctcggat gggagtacac
     4021 cgagaacatg caactcctgt ttcagcatgt tctcgccaag gtcaaccgag ccattctgct
     4081 gactagctct taagaactga cgaatcttgc tgcgagcccg cccagacttg acgtaagtca
     4141 gccagaccgg attcggactg gcctgcggcg cggtgatgat ttcaacctga ttgccattgg
     4201 ccagttcggt ggacagggaa atgagttcat gatcgatgcg ggccgcaacg caatggtggc
     4261 caatatccgt atggacggcg taagcaaaat caatgaccgt ggaaccacga gggagtgcaa
     4321 gtattttccc cttgggcgta aacacgtaca cctcgtccgg aaacagatca accttgacgt
     4381 gctcaagaaa ctcggaggaa tctccggtcg aactctgaag ttcaaggagc gaatgcagcc
     4441 acttgtgcat cttgacttgc aggtgggcgc cactgtcttc gctatccttg taaagccagt
     4501 gtgaggcgat tccctcctct gccagattgt gcatgctctg ggttcggatc tgaatctcga
     4561 ttggggtgcc gtaggggcca atgagcgtgg tgtgcaaaga ctgatagcca tttcccttcg
     4621 gaatggcgat gtaatccttg aactttccgg gcaccggctt gtaccgggaa tgcaatgaac
     4681 ccaaagccag gtagcaggtg ggaatgtcgc gaacgataat gcggtacccg tagatatcga
     4741 gcacctgcga gaagctgaga tgcttctcga ccattttgcg ataaatcgaa tacaggcttt
     4801 tctctcggcc gaacacgcct gcttctatat tgtcctcatg caacttgctg taaataccgt
     4861 ccagtgtctt ggagagcagc tcacgtcgat tgccgcgcac tgccttgatc gcgcgcttca
     4921 acacccggaa acgcatcgga tgaacgtgtt tgaaggcaag gtcctgcagt tgccgcgaga
     4981 tgttgttcag tccgagccgg gtggctatcg gcacgtagat ttccagcgtt tcccgggcga
     5041 tccgccgacg cttttcaagg cggatatggg ccatcgtttg cagattgtga aggcggtcgg
     5101 cgagcttgat catcacgatg cggagatcgt gcgccatggc catcagcatt ttgcggaaat
     5161 tctctgcctg cgctacctga aagggctgaa actcgatttt ctcgagcttt gaaagcccgt
     5221 cgaccaggtc ggccacttgt ttgccaaaac gaacggcgat ctcgtctttg gttaccgctg
     5281 tgtcctccat cacatcgtgc aggagcgcgg cgataacggc ctgtgcgtcc atacgccact
     5341 cagccagggc gccagccact gccagcggat gcgtgatata cggttcaccg gagaggcgag
     5401 actgcccctt gtgcgcttcc tcgctaaagc gataagaatc gacgacgagc gctatttcgt
     5461 ccggttcgag ataactgagg gtgtcgacga aaacccggta cgccgggtct tcattctcat
     5521 agattgcgct aactggctct ggctgagtag ttgagccgat agcaagcttg gcggctgtgg
     5581 cgtcggtatt cacgattgcg caccacgtag cctgccgggg tcagtcaggc gtgaccgcgg
     5641 ttcaaaactt ccaggccgta catgccaagc gccagctcgc gcagtgcaat gaccgttggc
     5701 ttgtcgcgat cgatatcgat catcggtgtt gcgccggcgg taatttgtcg ggcacgatag
     5761 gtggcggcca gggtcatctg gaatcggttt ggaatgaatt gcatgcagtc gtcaacggtt
     5821 acgcgtgcca tcggcttggt cctaaattca gagtagtgcg gcgaacagcg cagcgtggcg
     5881 ctgacactgg attgtgtatt tcagacgcga ggcgtttact atggactgaa gatcgtcaag
     5941 cgcccgcgat aactcgtcgt taataataac ataatcgaat tcatctgcat gccgcatctc
     6001 gtcgcgggcg gcgccaatgc ggcgtagtat ggtttcgacg gagtcggtgc cccggccgga
     6061 aagtctctct ccgagagctt ccagtgaagg cggcattacg aaaacgctga ccgcctccgg
     6121 gaaggacttt cgaacctgct gcgccccctg ccagtcgatc tccagtagaa cgtcacgccc
     6181 ggcatccatt tccatcttga tccactgcaa cgacgttcca tagtagttac catggacctc
     6241 agcccactca acaaactcgt tacggtgcac catctcgcga aagttctgga catcgacgaa
     6301 atggtactca cgaccatttt cctcgcctgg tcgtggtgtt cgcgtggttg tagatatgga
     6361 cacatgaatt cccggatcgt tcccgatcag cagacttacc agtgtcgtct tcccagcccc
     6421 cgatggggct gccacaataa aaagttgacc tggcataagc ttttacctgc agataatcgg
     6481 ggatgatcga cagacttgat tgtaccttcc tgatcagcaa aatctctccc ctagacgaca
     6541 agggacataa gggcgagaat caatgcgttt tcgaatcacc tgctcggaaa ggatttatgt
     6601 caagaagaat ggcgttgttg cccaagatgt ttctggtggc tgccctggcg acaacaggtg
     6661 ccggtgcggt cacgttgaaa gttgtcggct ttgacgacat gtcgtgccat gcctgggtac
     6721 aatcaaaaga cgattctgaa cagcgccgag tctacttggc ctggatacgt ggcgtgctga
     6781 ccggtcataa ctacgccaga ctgaagcagc aggtttcgga catatcggaa aacacggtgg
     6841 aaaacttcgt tgaccgctat tgcgtcgaaa aaccaaatgg ccagttcagc gatgcagcgt
     6901 tgcgtatgag tgacaggttt tcgggacgcg acgaagccat tacgcggtaa ggaatgc
//
LOCUS       DAORPM010000117         6937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2822_length_6937_cov_12.834060, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000117 DAORPM010000000
VERSION     DAORPM010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2822_length_6937_cov_12.834060"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1066
                     /gene="ppk2"
                     /locus_tag="PLD53_07000"
     CDS             152..1066
                     /gene="ppk2"
                     /locus_tag="PLD53_07000"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRC60697.1"
                     /translation="MSTDPTYEQRMNDAMLDSFDEELEMEFDDNRMDSLIDELASTGG
                     DETELGRNFYFKELFRLQGELVKLQDWVVHKKLKVVVLFEGRDAAGKGGVIKRITQRL
                     NPRVCRVAALPAPSAREQSQWYFQRYVSHLPAGGEIVLFDRSWYNRAGVEKVMGFCNE
                     DEYEEFFRSVPEFEKMLIRSGIVLIKYWFSITDDEQNLRFSLRIKDPLKQWKLSPMDM
                     ESRRRWEQYTKAKEIMLERTHIPEAPWWIVEAVDKKKARLNCIHHFLSQIPYEEIERE
                     SVVLPERVHNPDYLRHPVPKNMIVPPVY"
     gene            complement(1177..2916)
                     /locus_tag="PLD53_07005"
     CDS             complement(1177..2916)
                     /locus_tag="PLD53_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRC60698.1"
                     /translation="MNTFRNWRRSGVLLALALPFAISHALAEEKPAKAMSVEEKGYQA
                     GGSSPVGEAEMHQNINPKAPAMSKAEFDKAKNIYFERCAGCHGVLRKGATGKPLTTDK
                     TLAGGTEYLKVFIKYGSAGGMPNWGTSGEMTDDEVDMMARYIQQEPPVPPEWGMKEME
                     ASLKVIVPPDQRPKKKMNDIDINNLFSVTLRDSGQVALIDGKSKKIVTILPTGYAVHI
                     SRMSKSGRYLYVIGRDARVNLIDLWMPVPGNVAEIKVGLEARSVETSKYKGYEDKYAI
                     AGTYWPPQFVIMEGDTLKPLKIESTRGTTVDSQDYHPEPRVASIVASHYNPEFIVNAK
                     ETGKIMAVNYSDLNNLKITTIGAARFLHDGGFDSTGRYFLVAANASNKIAVVDTKEDK
                     LAALIDVGKIPHPGRGANFMHPKFGPVWATSDLGDDGISIIGTDPVKHKAQAWKVVQT
                     LKGNGSGSLFIKTHPKSTNLWVDAPLNPDAKISQTIAVFDIKNLDKGFEVLPIGEWSG
                     ITDDGARRVVQPEYNQAGDEVWFSVWSAKDKQSAVVVVDDKTRKLKAVIKDPKLITPT
                     GKFNVHNTQHDVY"
     gene            3141..4088
                     /gene="gluQRS"
                     /locus_tag="PLD53_07010"
     CDS             3141..4088
                     /gene="gluQRS"
                     /locus_tag="PLD53_07010"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRC60699.1"
                     /translation="MVHSALANSSAPIGYRGRFAPSPTGPLHFGSLVAAVGSYLDARA
                     NGGEWLVRIEDVDTPRSVPGAAALILRTLDDLGFEWNGEVLVQSRRLDLYHAALVDLQ
                     LAGEAYPCACSRSEIAAATSKKSVDGGLLYPGTCRTGLADGKAARAWRLRVPDREFVL
                     EDRVQGKTRQNLEREVGDFVLLRADGQYAYQLAVVVDDAAQDVNAIVRGVDLLDSTVR
                     QMWLQQRLGLPTPSYAHLPVVVNAQGEKLSKQTKAAAVDPSQGSSILASAMQFLGHPV
                     PTDIRHGPVADFWRWAISAWSIERIPSVKGIYPGPCVPP"
     gene            complement(4167..4952)
                     /locus_tag="PLD53_07015"
     CDS             complement(4167..4952)
                     /locus_tag="PLD53_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRC60700.1"
                     /translation="MTRLPLLHVVTYNIHKGFSQFNQHMMVHELREQLRAIGSDIVFL
                     QEVQGLHSRHARNIGNWPEASQHDFLAEDVWSATAYGSNVIYDHGHHGNAILSRFPIE
                     HSHNQDVTHMRFERRGLLHCAIKVPGLPKAVHCVCVHLSLFAHSRRRQMAALADYLAD
                     IADPDAPLIIAGDFNDWRNEADDLLATRLGLNEVFGGAGGSFGSPGRSFPAKLPVLRL
                     DRIYVRGFSVRHAQVHYGVPWSKISDHAALSAHLWHEAEGNSR"
     gene            complement(4958..6055)
                     /gene="nadA"
                     /locus_tag="PLD53_07020"
     CDS             complement(4958..6055)
                     /gene="nadA"
                     /locus_tag="PLD53_07020"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRC60701.1"
                     /translation="MNIAHIAFDRFNELQDKACHDRIRAARAKLGNKAVLLCHHYQRA
                     DVYQYADLTGDSLKLSRLASQAEAEYIVFCGVHFMAEVADIMSKPHQKAILPDLAAGC
                     SMADMANLAKVERCWRELNETLNAGETITPVTYINSAADLKAFCGDHGGIVCTSSNAG
                     TIAQWAFKQREKILFFPDQHLGRWTGYKMGIPLDEMMVWDPDLEMGGLTADEIRKAKL
                     LLWKGHCSVHQMFQPAHILKFRNQYPDGLVIAHPECSFEVCKQSDFVGSTEHIVKTIT
                     EAAPNTRWLVGTELNLVNRLAEEMRPEGKIVQFMAPTVCMCSTMQRIDPQHLAWTLEN
                     LAEGNIVNQIKVPEHEAILARLALERMLAAS"
     gene            complement(6116..6565)
                     /locus_tag="PLD53_07025"
     CDS             complement(6116..6565)
                     /locus_tag="PLD53_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRC60702.1"
                     /translation="MGKLTDLLNLARTRARELKLPYAGALTPKEAHEIWQLAPGAKIV
                     DVRTRAEWDWVGRIPGAEEIEWLHYPTNQPNSHFLAQLKQQVDPEALVMFICRSGARS
                     HNAACLASQAGYSECYNVLEGFEGDKDAHGQRGKTGGWRHSGLPWIS"
BASE COUNT         1439 a         2005 c         1947 g         1546 t
ORIGIN      
        1 acaaagtact gtctgccggg gaagcgcgcg cgcactcgta acacgactga aatactcgcc
       61 ttccgcgaat tgtgagaaca tcaggcaagt cttcggaccg ctatcgaatt tgagccatct
      121 gcacctgcat acaacccgta ctggagtacc catgagcacc gacccgacct acgaacaacg
      181 catgaacgac gccatgctgg acagctttga cgaagagctg gaaatggaat tcgacgacaa
      241 ccgcatggat tcgctgatcg acgagctggc aagtacaggc ggcgatgaga ccgagctggg
      301 ccggaatttc tatttcaagg aactgttccg tctgcagggc gaactcgtca aattgcagga
      361 ctgggtggtc cataagaagc tcaaggtggt ggtcctgttt gaaggacgcg atgcggctgg
      421 caaaggcggt gtcatcaaac gcatcaccca gcgcttgaat ccgcgcgtgt gccgggttgc
      481 cgcgttaccg gcgcccagcg cgcgggaaca gagccagtgg tatttccagc gttacgtatc
      541 ccacctgcct gccggcggcg aaatcgttct cttcgaccgt agctggtaca accgcgccgg
      601 cgtcgaaaaa gtcatgggct tttgcaacga agacgaatac gaggaattct ttcgcagcgt
      661 tcccgagttc gagaagatgc tgatccgctc aggcatcgtc ctcatcaagt actggttttc
      721 aatcaccgac gacgaacaga accttcgctt ctcgttgcgc atcaaggatc ctctgaagca
      781 atggaagctc agcccgatgg acatggaatc acgtcgtcgc tgggagcaat acaccaaggc
      841 caaggaaatc atgctggagc gcacccacat cccggaagcg ccgtggtgga tcgtcgaggc
      901 cgtcgacaag aagaaagcgc gcctcaattg cattcatcac tttttgagcc agattcccta
      961 cgaagaaatc gagcgggagt ccgtggtcct gccagagcgc gtgcataacc cggattacct
     1021 gcgacatccg gtgcccaaga atatgatcgt gccgccggtg tattgaagaa ggatcttgat
     1081 tcgggagcaa tacgagcatt actcggccgc acaactatgc gccagaatga aaaacggggc
     1141 gcatggcccc gtttttcatc gacagcgagt tacgacttaa tagacatcat gctgcgtgtt
     1201 atgaacattg aatttgccgg tcggcgtgat cagcttcgga tccttgatca ccgccttcag
     1261 cttgcgcgtc ttgtcgtcaa ccactacaac ggctgactgc ttgtccttcg cactccatac
     1321 cgagaaccag acttcatcac ctgcctggtt gtactccggc tggacaacgc gcctagcgcc
     1381 atcgtcggtg attcccgacc actcgccaat cggcagcacc tcgaaccctt tgtccaggtt
     1441 cttgatatcg aagaccgcaa tcgtctggct gatctttgca tccggattca gcggcgcgtc
     1501 aacccacaga ttggtggact tcgggtgcgt cttgatgaac agcgagccgc taccattgcc
     1561 cttcagcgtc tgcacaactt tccaggcctg cgccttgtgc ttgaccgggt cggtaccgat
     1621 gatgctgatc ccgtcgtcac ccaagtcgct ggtcgcccaa accggaccaa acttcggatg
     1681 catgaaattg gcgccgcggc ccggatgcgg aatcttgcca acgtcgatca aggcagccag
     1741 cttgtcttcc ttggtatcga ccacggcaat tttgttcgaa gcattggcag ccaccaggaa
     1801 gtagcgcccc gtcgaatcaa atccaccatc gtgaagaaaa cgtgcggcgc cgatggtcgt
     1861 gatcttcagg ttgttgaggt ccgaatagtt cacagccatg atcttgccgg tttccttggc
     1921 attgacgata aactcggggt tgtagtgcga agcgacaatg gatgctacac gcggctcggg
     1981 atggtagtcc tgcgagtcca ccgtcgtgcc acgcgtcgat tcgatcttca gcggcttcag
     2041 tgtgtcgcct tccatgatca caaactgcgg cggccagtag gtgccagcaa tcgcgtactt
     2101 gtcctcatag cccttgtact tcgacgtctc gaccgaacgc gcctccagcc cgaccttgat
     2161 ctcggcaacg ttgcccggta ccggcatcca cagatcgatc aggttgacgc gagcatcgcg
     2221 cccgatgacg tatagataac ggccggactt cgacatccgt gaaatgtgca ccgcgtagcc
     2281 ggtcggcaga atggtgacga tcttcttgct cttgccgtcg attagggcga cctgacccga
     2341 gtcgcgcagg gtgaccgaga agagattgtt gatatcgatg tcgttcatct tcttcttcgg
     2401 ccgctggtcc ggcggaacga tgaccttcag cgaggcctcc atttccttca tgccccactc
     2461 gggtggaaca gggggctcct gctggatata gcgcgccatc atgtcgactt cgtcatcggt
     2521 catctcgccc gacgttcccc agttcggcat gccaccagcc gagccatact tgatgaagac
     2581 cttgaggtac tcggtgccgc cggcaagtgt cttgtcggtg gtcagcggtt tgcccgttgc
     2641 gcccttgcgc agtacgccat gacagcccgc acagcgctcg aagtaaatat tcttggcttt
     2701 atcgaactcc gccttgctca tcgcaggcgc tttggggttg atattctgat gcatctcggc
     2761 ctcaccaacc ggcgaagagc cgcctgcctg ataacccttt tcttccacgc tcattgcctt
     2821 ggcgggcttt tcttcagcca gggcgtgcga tatcgcgaag ggtaacgcca gcgccagcag
     2881 cactccggac cggcgccaat ttctgaatgt gttcatgctc aaacctccca tggtgtattt
     2941 gaactacaga tacgtgctag caagttgctt ttcgctgccc accattttgc actaatagcc
     3001 tgaatacgtt ttgatattga tcaattctgc cccggttggc gtgacaggtg ccccacttca
     3061 acacatctgc aatctaaaat gctgctggcg acaacgctcg ctcaatattc agggtggagc
     3121 ttgcgatttt tagggaaacc atggtccatt ctgcccttgc caattccagc gcgccaatcg
     3181 gctatcgagg acgttttgcg ccctcaccca ccggcccatt gcattttggt tccctggtag
     3241 ccgcggtggg cagctatctc gacgcccgcg ccaatggtgg cgagtggctg gtacgcatcg
     3301 aggacgttga cacgccccgc tccgtgcccg gtgcagccgc attgattttg cgcacccttg
     3361 atgacttggg cttcgagtgg aacggcgagg tgcttgtcca gagccgtcgc ctcgacctct
     3421 accatgcggc actcgtggac ttgcaacttg caggcgaggc ctatccgtgt gcctgctcgc
     3481 gcagtgaaat cgccgcggcg acgagcaaga agtcggtcga tggcggactg ctctatcccg
     3541 gcacctgccg gaccggactg gccgacggca aggctgcccg cgcctggcga ctacgcgttc
     3601 ccgatcggga gtttgtcctg gaggatcgcg tgcagggtaa aacgcgacag aatctggaac
     3661 gcgaggtcgg agacttcgtt ctattgcgtg ccgatgggca gtatgcctac caactggcgg
     3721 tcgttgtcga tgatgctgca caggacgtaa atgccatcgt gcgtggtgtc gatctgcttg
     3781 attcgacagt gcgccagatg tggttgcaac aacgtcttgg cttgccgact ccgagctacg
     3841 cgcatctgcc ggtagtggtc aacgcccaag gcgaaaaact ctcaaagcag acaaaagccg
     3901 ctgcagtcga tccgtcccaa ggcagctcga ttctggcctc cgcaatgcag tttctcgggc
     3961 atccggtgcc gacggatatt cgccatggac ccgtggccga tttctggcgc tgggcgattt
     4021 ctgcctggtc gatcgagcgc ataccgtcag tcaagggcat ctatcccggt ccgtgcgtgc
     4081 cgccgtaaag caacgctatc ggtttgccat acgacaactg aatcgcgcaa tcgagcaagg
     4141 tgcgcccaat gaggctctgg accggctcaa cggctgttgc cctcagcctc gtgccacaga
     4201 tgcgcggaaa gcgcggcgtg gtccgagatt tttgaccatg gcacaccata atgcacctgg
     4261 gcatgtctga ccgagaatcc gcgcacatag atccggtcca gcctcaggac aggcagtttg
     4321 gccggaaagc ttcgccccgg cgatccgaag ctaccgcctg ccccaccgaa gacttcattt
     4381 aggccaagtc gcgttgccag caggtcgtcc gcctcgttgc gccaatcatt gaagtcgccg
     4441 gcaatgatca gcggcgcatc ggggtcggcg atatctgcca gataatcggc cagcgcagcc
     4501 atttgccgac gccgcgagtg agcgaacagc gaaaggtgaa cacaaacaca gtgaactgct
     4561 ttcggcagac ccggaacctt gattgcacaa tgcaaaaggc cacgccgctc gaaacgcatg
     4621 tgggtgacat cctgattatg tgaatgctcg atcgggaagc gcgaaagaat cgcattgcca
     4681 tggtgtccgt ggtcgtagat cacgttgctg ccgtaggctg tggccgacca gacatcttcg
     4741 gcgagaaaat cgtgctgtga agcttccggc cagtttccga tgttgcgcgc atgtctgctg
     4801 tgcaggccct ggacttcctg caagaagaca atatccgacc ctattgcacg cagctgctcg
     4861 cgcagttcat gcaccatcat gtgctggttg aactgggaga agcctttgtg aatgttgtag
     4921 gtcacgacgt gaagcagcgg aagtcttgtc atggcgctca agatgctgcg agcattctct
     4981 ccagtgccag cctggcgagg atagcttcat gctccggcac cttgatctga ttgacgatat
     5041 tgccttcggc cagattctcg agcgtccagg ccagatgctg aggatcgatg cgctgcatcg
     5101 tagagcacat gcaaacggtc ggcgccatga actgcacgat tttgccctcg ggccgcattt
     5161 cctccgcgag ccggttgacc agattcaatt cggttccaac cagccagcgc gtattcggcg
     5221 ccgcttccgt gatcgtcttg acaatgtgct cggttgagcc gacaaagtcc gactgtttgc
     5281 aaacctcgaa actgcactcc gggtgcgcga tgaccagccc gtcagggtac tggttgcgga
     5341 acttcaggat gtgcgccggc tggaacatct ggtgcaccga gcagtgccct ttccagagca
     5401 acaatttggc tttacgtatc tcgtcggcgg tgaggccgcc catctcgaga tcgggatccc
     5461 ataccatcat ctcgtcgagc ggaatgccca tcttgtaacc tgtccagcgc ccgagatgct
     5521 gatcggggaa gaacagaatc ttctcgcgtt gcttgaatgc ccactgggca atcgttccgg
     5581 cgttcgacga agtgcacacg atgccgccgt ggtcgccaca gaaggccttg aggtctgcgg
     5641 cggaattgat gtaagtcacc ggcgtgatgg tttcgccggc attcagcgtt tcattgagct
     5701 cgcgccagca acgttcgacc ttggccagat tggccatgtc ggccatcgag cagccggcag
     5761 caaggtcagg cagaatggct ttctggtgtg gcttggacat gatgtcggcg acttcggcca
     5821 tgaaatgcac accgcaaaaa acgatatatt cggcttcggc ttgcgacgca agccgcgata
     5881 gcttgagcga gtcgccggtc agatcggcgt attgatacac gtcggcacgc tgatagtgat
     5941 gacagagcag gacagccttg ttaccgagct ttgcacgggc agcgcgtatg cgatcgtggc
     6001 aagccttgtc ttgcagttcg ttgaatcggt caaaggcgat atgtgcgatg ttcatttacg
     6061 gatctcgaat ctgttcgatg cctgaataag cagactgtcg ctgatcaaaa aggtttcagc
     6121 ttatccacgg caggccgcta tggcgccaac cgccagtttt tccgcgttgt ccgtgggcat
     6181 ccttgtcgcc ctcgaacccc tcaagtacgt tgtagcattc ggaatagccg gcctggctgg
     6241 ccagacacgc ggcgttgtgc gagcgggcac cgctgcgaca gatgaacatg acgagcgcct
     6301 cgggatcaac ctgctgcttg agttgtgcca ggaaatggct attcggctga ttggtcggat
     6361 agtgcaacca ttcgatttcc tcggcaccgg gaatgcggcc cacccagtcc cactcggcac
     6421 gggttcgcac gtcgacgatt ttggctccgg gcgccaactg ccagatttca tgggcctcct
     6481 tcggcgtcag tgcgccagca tagggaagct tgagttcgcg ggcacgcgtc cgcgcaagat
     6541 tcagcaggtc ggtcagtttt cccataagta tcgaagcaat ccagattagg gaaacgaagt
     6601 ataaatcact tgatctgccc cttgctgttt cagcaaggat tatgctgctg caccaatgtg
     6661 gtgcgcttgt ctcaatttac gcactggtat tgcgcatagc acatgtcctg aacgtacaaa
     6721 atgcctctag ttgctaggcg ggttcccgga gcgctgtttg gcatggtttc tgctaatatt
     6781 catcgtacct tttttcattg tcaccggtgt caccggtatc cgctgaggag atttttcata
     6841 tggcaagccc gcaagacgtt atcaatatgg tcaaggaaaa cgacgtcaag ttcgtcgact
     6901 tccgttttac cgacacccgt ggcaaggagc agcacgt
//
LOCUS       DAORPM010000118         6920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2835_length_6920_cov_8.242535, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000118 DAORPM010000000
VERSION     DAORPM010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_6920_cov_8.242535"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..372
                     /locus_tag="PLD53_07030"
     CDS             163..372
                     /locus_tag="PLD53_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60703.1"
                     /translation="MPSLNANHHPLMSRMHKPDAKLAPDQQDERSVISIELADVDAWL
                     RGLTDEAARLVRVPPGEVFDAAPAK"
     gene            complement(518..1405)
                     /locus_tag="PLD53_07035"
     CDS             complement(518..1405)
                     /locus_tag="PLD53_07035"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRC60704.1"
                     /translation="MVLKPGDGASIIAPASQLRGGEQGLLGAAVNLLESWGLRVFVRV
                     DNTHHFYLAGPDAVRAEHLNTSLADTDTKVIFCLRGGYGCLRLLPYLNGDMSPPDKIL
                     VGYSDFTGLHLAARKLWPQVELIHGPNVATRQLIGDEIDCALTRQSLHNALFAPETVE
                     EQVEFLRPGSAEGRLEGGCLSLVVATLGTKYALNTVDAILFLEDTGEAPYRIDRMLTH
                     LRNAGKFEAVRGIVFGKMRNCTDPYNDLRDVLRDFFQDAPFPVAFGLNSGHGPSNLSL
                     RLGTTAELISEGLCFRMEP"
     gene            complement(1408..2139)
                     /locus_tag="PLD53_07040"
     CDS             complement(1408..2139)
                     /locus_tag="PLD53_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRC60705.1"
                     /translation="MNDYERIPEIHPDLPADAVAVDECGEDLCTLTTTAKICVAPAYH
                     ARGFTSAPNAVQVRTTVRDALHRAALGLPTGLFLLVWDGLRTLQTQREIAQRFAISLR
                     LCADEKERETLFQRYVSPLPKSLAQFTRQPPPHATGGAVDLSLCDADGNSLDLGAEFD
                     QFDENAWLTWFEHRCSNPRSSRADNKRRRLRRILYWAMVGAGFAPYAWEYWHFELNTR
                     AAAAFYHRDVAAYGPAVPWSGNSGD"
     gene            complement(2136..3128)
                     /locus_tag="PLD53_07045"
     CDS             complement(2136..3128)
                     /locus_tag="PLD53_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60706.1"
                     /translation="MTRKALLVIGSRTRENGYGITSGLRVAEALRRRGWQADTIQATD
                     SRLLLQLLLHGSFDVVVPVGFGAPCEDGHVFAAARLAGIPCAGPTPAAGSIMLDKSMT
                     SRLVDALFAPSSGVRSPSGRTLAKGQSTANIELLIRQLTPPLVVKPNFSGSSDGLYVA
                     STYVEALAAVCGMLAYEGRVCVQTLEHPVAHEVSCTVLDAPDGPLFLPIVELRRDDVH
                     VLGAAEKFGSEGLNRHIIPARLRTETVAKLKHVVLTLHEEIGATGLTRTDVLVLPDDT
                     LVVLEINGIPGLLESSIACDAAHAAGICFDELCIKYAESAYLPRAEPRIWEGLP"
     gene            complement(3125..3913)
                     /locus_tag="PLD53_07050"
     CDS             complement(3125..3913)
                     /locus_tag="PLD53_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60707.1"
                     /translation="MLSYAACAAEAIRSDVHAIVGQPHQIVIFSSGPKAGRTDVFSSL
                     AGIGALAGALRLARVDIPIVLDVASAADELPQGLPLALPEPLATWVFHRAEIHGADLT
                     NPYRYAYEHASPTMFGDLLASQTSAIRMTVGGATEAPFWAVRMLVRQTALARGYFVAP
                     AAGVILKALKRPWYSATQLEPPLAMMQEPKLAITALDRAANPERGGNVGLCREARALR
                     QFVNNPGLAGFLDAMHSVDTIARFVRASGFVVGPRLAELMGGNK"
     gene            4841..5132
                     /locus_tag="PLD53_07055"
                     /pseudo
     CDS             4841..5132
                     /locus_tag="PLD53_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235906.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(5483..5833)
                     /locus_tag="PLD53_07060"
     CDS             complement(5483..5833)
                     /locus_tag="PLD53_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60708.1"
                     /translation="MEQVTALELRKNLSEIARQVRDESQKKMLVVHGEPNIALVPVSW
                     GRVADQADEISAQHDISMNKELADIFRRQAEIIDESSVPVSLSTMINTFVDHATIVSK
                     YKELTKRRSSISQD"
     gene            complement(5880..6224)
                     /locus_tag="PLD53_07065"
     CDS             complement(5880..6224)
                     /locus_tag="PLD53_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60709.1"
                     /translation="MKPQQVSRALAGQASGSHLAASLHSLFHMVGSELPAADLRRLVV
                     KISRNLCSAISNAIRRFEPAEAENLHIVQLSHRINVRSSDGCVVLIIWFVFFVSVGFV
                     HMVEPMVDHRLV"
     gene            6444..6911
                     /locus_tag="PLD53_07070"
     CDS             6444..6911
                     /locus_tag="PLD53_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60710.1"
                     /translation="MVKVSSAGTSGEVMEQHDMVEGVAGKRYTVGKDMRLAASANDAV
                     EELVGDFVLTDERGARLFGAMPCLNTQPETAIDWLLLGLPLTTDEAKSDELDKRVVGR
                     HIVTPNESRPISETQGSSCNRSVRGSNTADPGGIGPRGSQRPRQASSFQAVFD"
BASE COUNT         1513 a         2007 c         2007 g         1393 t
ORIGIN      
        1 gtgatttcca ggacggccat gggccgattt tactattgat aaaatatgtt atcaagagtt
       61 agcgccaatt gcagcgaggc ccgatgagcc ggccatgaga tcgacgggct atggaatgca
      121 tggaccgaca agggcaccgg taaagtcatc gagagctata cgatgccctc cctcaacgcg
      181 aaccatcacc cgctgatgtc gcgcatgcac aagcccgacg caaaactggc gccagaccag
      241 caagacgaac gcagtgtcat ctccatcgag ttggccgatg tggatgcttg gctgcgcggg
      301 ttgaccgacg aggcggctcg gttggtgcgg gtaccgcctg gcgaggtgtt cgacgccgcg
      361 cccgcgaagt gaaacgaggt ccgcaaccaa gccaccgccg ccccgccgaa ggagggcgga
      421 acgtgagatc ggcaaccccg ccttgcgccg ggcgtcccgc ttttgacact tcggcacggt
      481 gtgttcaatg tcggccgaac agcgttcgtg gccgccttta tggctccatg cgaaagcata
      541 gtccttcgct gatcaactcg gcagtggtac ctaacctaag agacaaatta cttggaccgt
      601 gtccgctatt gaggccaaag gcaaccggga agggcgcatc ctgaaagaaa tcgcgtaaca
      661 catcgcgcaa gtcgttgtac gggtcggtac agttccgcat tttcccaaaa acaatgccgc
      721 ggacggcctc aaactttccg gcatttctca aatgcgtcag catgcggtca atgcggtagg
      781 gggcctcacc tgtgtcctcc aagaaaagaa tggcatcgac tgtgttaagc gcgtactttg
      841 tgcccaatgt cgcgactacc agcgatagac atcctccctc cagacgccct tcggcgctgc
      901 cggggcgcag gaactcgact tgttcctcca ccgtctctgg ggcgaacaag gcattgtgca
      961 aagattgacg agtcaacgcg caatcgatct catcgccaat gagctggcgg gtggcaacat
     1021 tcggtccatg gatgagttcg acctgcggcc ataactttct cgcagcgaga tgcagccccg
     1081 tgaaatcgct atagcccaca aggattttgt ccggtggcga catgtcacca ttcaggtatg
     1141 gcaggaggcg caaacagccg tagcctcccc gcaagcagaa gatcactttt gtgtcggtgt
     1201 cggccaagct tgtgttgaga tgttcggcac gaacggcatc tggccctgcg agatagaagt
     1261 gatgcgtgtt gtcgacgcgc acgaaaacgc gcaagcccca ggattccagc aggtttacgg
     1321 ctgcccccag gagaccttgt tcgccaccgc gaagttgcga agcgggcgcg ataatcgatg
     1381 ctccgtcgcc gggcttgaga accatggtca atctcccgag tttccgctcc aaggcacagc
     1441 tgggccgtat gccgcaacat cccggtggta gaacgcagca gctgcacgcg tgttcagttc
     1501 aaagtgccaa tactcccagg catagggcgc gaagccggcg ccgaccatgg cccaatatag
     1561 aatgcgccgc agacggcggc gtttgttatc ggcacgtgat gatctcggat tgctgcagcg
     1621 gtgctcgaac caggtaagcc acgcgttctc atcgaactgg tcaaattcgg caccaagatc
     1681 aagcgagttg ccatcggcat cgcacagact tagatccacg gcgccgccgg tcgcgtgcgg
     1741 cggtggctgc cgtgtaaact gcgccagcga ctttggcaat ggcgatacgt atcgttggaa
     1801 cagtgtttcg cgttccttct cgtcggcgca gagacgcagg gaaatcgcaa agcgctgtgc
     1861 gatctctcgt tgtgtttgga gcgttcgtag tccgtcccaa accaagagga acagacccgt
     1921 cggcaaacca agcgcggcac ggtgcagtgc gtcacggacg gtcgtcctga cttggacagc
     1981 gttgggggcg ctcgtaaacc cgcgcgcatg gtaagccggg gctacgcaga ttttcgccgt
     2041 tgtggtcagg gtgcataggt cttcgccgca ctcatctacc gccacagcgt cggcgggtag
     2101 atcaggatgg atttccggaa tgcgctcgta gtcgttcacg gcaaaccttc ccagatccta
     2161 ggttcagccc gcggcaggta tgcggattcg gcgtatttaa tgcagagttc gtcgaaacag
     2221 atccctgccg catgcgccgc atcgcaggca atcgagctct ccagcaagcc cggaatgccg
     2281 ttgatttcga gtacgacgag cgtgtcgtcg ggcagaacaa ggacgtctgt gcgcgtaagc
     2341 cctgtggccc cgatttcctc gtgtagagtg agtacaacgt gtttgagttt tgctacggtc
     2401 tccgtccgaa gtcgcgccgg aattatgtgg cggttgaggc cttcactccc gaatttttcg
     2461 gcggcgccaa gcacgtgcac atcatctcgc cgcaactcaa caattggcaa aaacagcggc
     2521 ccatcgggcg catcgagtac cgtgcagctc acctcgtgtg cgactggatg ctctaacgtt
     2581 tgcacacaca ccctgccctc gtaagcgagc atgccgcaaa cagccgcgag cgcctcaaca
     2641 taagtcgaag cgacgtaaag gccgtcgctc gagccggaga agttaggttt aaccacgagc
     2701 ggtggcgtca gttgccgaat cagaagttcg atattcgctg ttgactgccc ttttgcgagc
     2761 gtgcgccctg acggcgaacg aacaccactg gatggtgcga acagcgcgtc aaccaagcgc
     2821 gaggtcatcg atttatccag catgatgcta ccggcggccg gcgtcggtcc ggcacacggg
     2881 atgccggcaa ggcgtgcagc ggcgaataca tgtccgtctt cgcaaggggc gccaaagcca
     2941 actgggacca cgacatcgaa cgacccatgg agtaagagtt gcagaagcaa acggctgtcg
     3001 gtcgcctgga tcgtatcggc ctgccaaccg cgccggcgaa gtgcctccgc cacgcgcaga
     3061 cctgaagtta ttccgtaccc gttctcgcgc gtgcgcgagc cgatgacaag cagtgccttc
     3121 cgggtcattt gtttccgccc ataagttcgg ctagccgcgg cccgacaaca aatcccgagg
     3181 cgcgaacgaa gcgggcgatc gtgtcgacgc tgtgcattgc atcgagaaaa ccggccagtc
     3241 ccgggttgtt cacaaattgg cgcagggcgc gagcctctct gcacaagccc acgttgccgc
     3301 cacgctccgg attcgccgcg cgatccagcg ccgtaattgc cagtttcggc tcttgcatca
     3361 tcgccagggg tggctccaac tgggtggctg aataccatgg gcgtttaagc gccttcagaa
     3421 tgacgcctgc tgcgggcgcg acaaagtacc ctcgcgcgag agcggtttgc cgtaccagca
     3481 ttcgaacggc ccaaaacggc gcctctgtgg ctccgccgac cgtcatgcgg atagcggatg
     3541 tttggctagc aagaagatcc ccgaacatcg taggagaggc gtgctcgtag gcgtagcggt
     3601 acggatttgt cagatcggcg ccgtgaatct ctgcgcggtg aaatacccag gtggcgaggg
     3661 gttcgggaag tgcaagggga agcccttgcg gcaattcatc ggctgcgctc gccacgtcca
     3721 aaacaattgg tatgtccact cttgctaacc gaagcgcgcc ggccaaagca ccaatgccgg
     3781 ccaatgaact aaatacgtcc gtcctgcctg cttttggtcc cgaggagaag atcacaattt
     3841 ggtgaggctg tccgacaatc gcgtggacat cggagcgaat tgcctccgcg gcgcacgccg
     3901 catagctcag cacgcgagcg gggatcccgt cggaaagcgg cccgacccca atgcgcacct
     3961 cgaccctttg attaccgacg cgcgcccgct ggaccgcacg gacggtttgg tccaagtctg
     4021 tttcgccctg tggccatgcg acggcgacag aatcggaaag caccatcatg aaaccgactc
     4081 ctgcgccctg aggctggtgc cggccgcaat tatccggccc atcgcgtcaa caacgtgccg
     4141 cgaatgaacc cctagcgggg tcgcaaaaag cacacccgca gccgaggtag gctggcctac
     4201 tctatcgagg cgcgtgcctc caacggagga aaagcccgtt ccaagttgct gccgttgcat
     4261 cgttgtcatc gctgtgaccc ccatagccgt agttaactaa taatcacact atgagtatct
     4321 caaatactac taaatatgtc aatcataaaa tatcaaaata gtgaataaaa agttaacata
     4381 agcggcagag gctctcctgg acgatgatct tcggcccgtc caagcatgtg catgtcggtg
     4441 gcgccggccg cctggcagac ccgcgtaccg gacgggatga tcatgaccgc cgcagttcgg
     4501 cgagaagaag gcgaagcgtc cgtgatccgg ctggattcga tcagcaggca ccagaggcac
     4561 tctgtgctgc ccgggccgga cgcttacgct gattccgatg gcgtgactga attccgtgat
     4621 ttgtcggaat cggcagttac aatttcggag cgagcattgg atgtgtggca aagacgttta
     4681 gcgagacggc caccctaacg gtatattttt acaaggaacg gatgctggcg ggtaacggat
     4741 tgccatgcac aggatcaagg atctgttgcg gcttgagagg aatggcgccg cccctgcgct
     4801 cctctgtgaa gaatatcgac aggcgcaagg cgaggcggac ttccgaatac tcgcgcttct
     4861 atgatttcta ccgcgaattc gccaagcgcc tcaaatgctc gatgcggcag atccatcgcg
     4921 ccggcgagaa ctgttcgccg acttcgccgc tacaccgtgc cgatcgtcga tgccggcagc
     4981 ggcgagatcc ggcgcgcgca tcgtcgtcgt ccgcggcgcc tcgaactacg cctacgcctg
     5041 cgtgacggcc tgattgtccc caggacagcg ccaagatcga agttggccta cagatcgtcc
     5101 agtgctggat tctcgtccgc ttgcgcaacc gacgtagtgg aatacgtgca aagccgactc
     5161 gattactacg tctgagcgat gagcattact acagcgtcac caatgcgctg gtgctcgaaa
     5221 aaatcaatgt ccagacgacc ggatcgttcg agggttttcg tccggggcaa gcgcgttccg
     5281 cgcacgcatg tgcactaccg acgtggtttt cacgccacct tgcccgaaca aatgttggcc
     5341 tcgcagtggg cgcattcgga gtagtcaccg gccggatgct caactcggcg atgacgaatg
     5401 gtcacgcact gatgatgtcg tgcgctacca actcgaatcg cgcccgcgcg ccgtcgtgtt
     5461 cggtgcgatg cgctaccggc gatcaatctt gcgagatcga gcttcgccgt ttcgttaact
     5521 ctttgtattt gctaacgatc gtcgcgtgat cgacgaaagt attgatcata gtactgagcg
     5581 ataccggcac gctggattcg tcaatgattt cggcttgcct gcggaagatg tcggccaact
     5641 ccttgttcat gctgatatcg tgctgggccg agatttcatc ggcttgatca gcgacccttc
     5701 cccagctgac tgggaccaga gcaatgttcg gctctccatg cacaacgagc atcttcttct
     5761 gactctcatc gcgcacttgg cgagcaatct cggaaaggtt ctttcgcagt tctagtgctg
     5821 taacttgttc catatatggg cctataaatt ttatggagca cacttggtgc ccctcttggt
     5881 taaacgagcc tgtggtccac cattggctcg accatgtgta caaaacctac ggaaacgaag
     5941 aacacgaacc aaatgatcag tacaacgcat ccatcggaag atcgcacgtt gatcctgtgt
     6001 gaaagctgga cgatgtgcag gttttcggct tccgcgggct cgaacctccg aatcgcattg
     6061 gagatggcag agcaaaggtt gcgcgagatc ttcaccacca ggcggcgaag gtccgctgcg
     6121 ggcagttcac tgccaaccat atgaaataga ctatgaaggc tagcagctaa gtggctaccc
     6181 gaagcttggc ctgccaacgc tcgcgatacc tgttgcggct tcatgacgaa ctccaaaaat
     6241 atggggctgc acaccaatca gtaaacacta gacttctata tataaatgac taagttgtca
     6301 atatttatgt aacgtatttg ttgagctgcc aaagcaacgc gttgatactg cctcttcatt
     6361 tcatgagaga aggttcggtt ttgagaggag cagatgtcgc gtggtcgttg cgttcctcac
     6421 aggaacggaa tgtggcgatg aacatggtga aagtatcctc cgcgggcacc tccggcgagg
     6481 tgatggagca acacgatatg gtcgaggggg tcgccggcaa gcgatacacc gtcggaaaag
     6541 acatgcgact ggccgcgagt gccaacgatg ccgtggaaga actggtcggc gatttcgtgc
     6601 taaccgacga acgcggagcc cgactctttg gcgcgatgcc ctgcctgaat acccaacctg
     6661 agacagcgat cgactggctc cttctcggtt tgccgcttac caccgacgag gcgaaaagcg
     6721 acgaactgga caagcgtgtc gtcgggcgcc acatcgtcac acccaacgag tcgaggccga
     6781 tatccgaaac gcaagggtct tcctgcaacc gctccgtgcg gggttccaat acggcagacc
     6841 ctggtggaat cggacccaga ggtagccaga ggccccgtca agcctcgtcg tttcaggcgg
     6901 ttttcgacta atgtcacacc
//
LOCUS       DAORPM010000119         6882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2870_length_6882_cov_9.226600, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000119 DAORPM010000000
VERSION     DAORPM010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2870_length_6882_cov_9.226600"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..607)
                     /gene="kefF"
                     /locus_tag="PLD53_07075"
     CDS             complement(47..607)
                     /gene="kefF"
                     /locus_tag="PLD53_07075"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HRC60711.1"
                     /translation="MILIIHAHPYPQRSRAGRALLEAVRDLPDLEVRSLYDLYPDFDI
                     DVAAEQAALARADLVVWLHPIYWYSAPAMLKHWFDVVHLRGWAYGEGGNALRGKHCLW
                     VATTGGEASSFSESGAHRLPFAAFEAPIRQTAKFCGMVWEPPIVLHGANVVSDDALEA
                     AAIVFRKRLAAWAPSPARGEAFPEVE"
     gene            complement(755..2509)
                     /gene="phaC"
                     /locus_tag="PLD53_07080"
     CDS             complement(755..2509)
                     /gene="phaC"
                     /locus_tag="PLD53_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRC60712.1"
                     /translation="MNTEQSAQSAASALNFFQVGQALTQACLDFVTKQQLAVLLPASA
                     PLAPSAPLPMPNLEPLASLQQELATGHAHLVQKMMESWSGMAPEPVVRPEGGDRRFKA
                     PEWSESPFYSYLQQAYLLNASYLTKLAEALPINDGPTRTRVQFLTRLYVDAMAPSNFA
                     ATNPEFIKTALETKGESITAGIRNMLADLEKGHISMTDESAFEIGGNIATTPGSVIYE
                     NDLMQLIQYHPSTSKVAERPILLVPPCINKYYLMDLQPENSFVGYIVEQGFTVFLISW
                     RSATEDQGHYTWDDYLEMGPIKALKVVREVTGVAKPNALGFCIGGPLLCSALAVLKAR
                     GEDPVESLTLMTALLDYSDPGEIGALVTEATVTAYEASIGKGGVLHGRELSNVFSALR
                     ANDLIWQYVVGNYLKGGKPPPFDLLYWNSDSTNLPGPCFTWYLRHTYLTNMLRIPGKL
                     EMLGEKVDLGKIKVPAYIMAAREDHLVLWQAAYLSRKILGGPTLFTLAASGHIAGSIN
                     PASKNRRSYWVNDSEALLTSDEWLASATEKRGSWWPHWAEWLGRRSGKRIAPPRTTGS
                     TSYGEIEPAPGRYVKVRI"
     gene            2848..3915
                     /locus_tag="PLD53_07085"
     CDS             2848..3915
                     /locus_tag="PLD53_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60713.1"
                     /translation="MKYVNHSNLFLPLLFLLLMVIAGGVAAQTVDGARITSTCQVERP
                     SDSGHGARSEDAPSGSGLSFSIGGFALGGSRPRSPSYESESENTPREESHSHGNVGVG
                     LSINLGELFRHLGKPEIPQQLFEDGPRFESAFSMACIPVRGFVRGGWPLVIDYQADPG
                     TVASVEIHVEGRDPVVIPLRRGSEHNLLKLRLPDQLGNELSPALLLVRAVKDQPGAPV
                     LGRINVFGLGAGPRAVGSVAIDQVDFQPGTMSLGKKETARFSFYSRSDFNRAAAEVLR
                     VINRDGDLQVRLARSIPFDGGVAAGTWVGRDKPLIWDGTDADRQPSLGQHLMQVRAWL
                     SAQAGGDWVAAWSAWTVEVTR"
     gene            3920..5479
                     /locus_tag="PLD53_07090"
     CDS             3920..5479
                     /locus_tag="PLD53_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRC60714.1"
                     /translation="MFASILRYRWLRELRALWPWAVGLLLLALAGQLALTSPRTSVNL
                     DSIGSLRALPGQAITLSHPVSDRVLVTYTSGREGGLDLRFEQARLAPETLELLRRVGI
                     MAPAVEAPASWITRAEPGIGAESMSSFEVAAVDSGKLPRTLLLRVLAGATPRQVRLEL
                     LARGAPLEVTLALPWTGDAHAPRKMLRLGETELLLPGALPIKLLVPPEASMRAIISLP
                     DKPQASQSADLEFGTFTGREGGGLAVSALGIEAAPGAGSLLACGAPAGSLLWRAAGRL
                     AAGDCPESVGSISVRSLELRADMADLTVGGNAWVLKDGQPLGEDLLSRLRQQPVWWAL
                     LLLVDGLLLLWALLAIVPGPRRITVRGVFISYRRADSGPRVGRLHDRLVARLGAERVF
                     LDLESIPAGEDFDRFIADGLSRVDIVLAVIGPQWLELRDEHGQRRLDAESDLVRREIA
                     AALLAGVRVIPVLVGGAPMPTADQLPSDIAALAGRNAMTIGDLKFLRDADDLIDQFEY
                     APVHSTGNGTP"
     gene            complement(5487..6581)
                     /locus_tag="PLD53_07095"
     CDS             complement(5487..6581)
                     /locus_tag="PLD53_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRC60715.1"
                     /translation="MKELAERFGFSFLAEPWLAKALIAIVATVALNFFANWLLRKATR
                     KPKDSTDAWNDILFHAARRPLPAVIWVVGIAFAAGIVGRHADDPLFDLFPPLRDVGVV
                     TCFAWFLFRMIHLASAHYVSRKVREGDEVDKTTVDAFSKLGRLTVVLTAALVIMQTLG
                     FSVAGILAAGGIGGIAVGFAAKDIIANFFGGLTIYLDRPFSVGDWIRSPDKQIEGTVE
                     VISWRHTRIRAFNKNPIYVPNAVFTTIVVENPSRMSHRRIKETVGIRYADIDKMAAIV
                     ADVRAMLEQHPEIDAKQTLIVNFDKFADSSLNFFIYTFTNTTQWVHYHEIKQDVLLRI
                     AGIIARHRAEIAFPTQTLHIEQTLEEPPLK"
     gene            complement(6646..>6882)
                     /locus_tag="PLD53_07100"
     CDS             complement(6646..>6882)
                     /locus_tag="PLD53_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60716.1"
                     /translation="TWQTRAMIVAPVVLGMKVEDSSSKQGLASSHFWTDALKAPVKPL
                     PGRMLVVCFFCQTLSTSVKRRAGRVARCQRLTYA"
BASE COUNT         1355 a         1962 c         2124 g         1441 t
ORIGIN      
        1 acgcagatga ctgcagcggc aaggtagatc agtgcatcgg tcaacatcac tcaacctccg
       61 gaaatgcctc cccacgtgca ggagaagggg cccacgcggc caatcgtttt cgaaagacga
      121 tagccgccgc ttcgagtgca tcatccgaga ctacatttgc gccatgcagt acgattggtg
      181 gctcccagac catgccgcag aatttggctg tctgacgaat cggtgcctcg aaagccgcaa
      241 agggcaaccg atgggcaccg gactcgctaa acgatgacgc ctccccgccg gtggttgcca
      301 cccagaggca gtgtttcccg cgcagtgcat tgccgccttc gccgtacgcc cagccgcgca
      361 gatgcaccac atcaaaccag tgtttcagca ttgccggtgc gctgtaccaa tagatcgggt
      421 gcagccagac caccagatcc gcgcgcgcca acgcggcttg ttcagcggcg acgtcgatat
      481 cgaaatcagg atagagatcg tagagcgagc ggacttcgag atcaggaaga tcgcgcactg
      541 cctcaagcaa ggcgcggccg gctcgggaac gctgcggata aggatgcgcg tggatgatca
      601 gaatcatcgg cgaagaatat cgcactatcc cgaaaatcag ctatctcgac gacgccccat
      661 ttcgcccacc tggattgaac tcaggccgga agtagcaaag atggctctga cctggctctg
      721 caggatgcct cttgcattga ttggccgcag gaaactagat cctgaccttg acgtagcgtc
      781 ctggcgctgg ctcgatctct ccatagcttg ttgagccggt agttctgggt ggcgcgatcc
      841 ttttgccact gcgccggccg agccattcgg cccagtgcgg ccaccagctc cccctcttct
      901 cggtggcgct ggccagccac tcatcggagg tgagaagtgc ctcactgtcg tttacccagt
      961 agctgcgccg gttcttcgac gccgggttga ttgatcccgc gatatgcccg ctggcggcca
     1021 gcgtgaagag cgtcggaccg ccgagtatct tgcgcgacag gtaggccgct tgccagagca
     1081 ccaggtggtc ttcgcgtgcc gccatgatgt aagccggcac cttgattttc cccaggtcga
     1141 ccttctcgcc gagcatttcc agcttgcccg gaatgcgcag catattggtc agatacgtgt
     1201 ggcgcaggta ccaggtaaaa cagggccccg gcagattggt gctgtccgaa ttccaataaa
     1261 gcaggtcgaa gggcggcggt ttccctcctt tgaggtagtt gccgacgacg tactgccaga
     1321 tcagatcgtt cgcgcgcaag gcagagaaga cgttcgagag ctcgcgccca tgcagtacgc
     1381 cgcccttgcc gatcgatgcc tcgtaggctg tgactgtggc ttcggtcacc agggcgccga
     1441 tttcgccagg atcggagtaa tcgagaagtg ccgtcatcaa tgtcaggctc tcgaccggat
     1501 cctcgccgcg cgccttgagc accgccagag cagaacacag aagcggccca ccgatacaga
     1561 agccgagggc gtttggcttc gcgacgccag tgacctcgcg caccaccttc agtgccttga
     1621 tcggccccat ttcaaggtag tcgtcccagg tgtagtggcc ctgatcctcc gtggcgctgc
     1681 gccaggaaat caggaagacc gtgaaaccct gctcgacgat gtagccaacg aaggaattct
     1741 cgggttgcag atccatgagg tagtacttgt tgatgcaggg cggtacgagc agaatcggcc
     1801 gttcggcaac cttgctggtg ctcggatggt actggatcaa ttgcatcaga tcgttctcgt
     1861 agatcacgga tccgggcgtc gtcgcaatgt tgccgccgat ttcgaatgcg ctttcgtcgg
     1921 tcattgaaat atggcccttc tccagatcgg ccagcatgtt tctgatgccc gcagtgatgc
     1981 tctctccctt ggtctcgagg gctgtcttga tgaactccgg attggtcgcg gcgaagttgg
     2041 acggcgccat ggcatcgaca tacaggcgcg tcaggaactg cacgcgtgtc cgggtgggcc
     2101 cgtcgtttat cggcagcgct tccgcgagct tcgtcagata gctggcgttg agcagatagg
     2161 cttgttgcag gtaagaatag aaaggacttt cgctccactc tggcgctttg aagcggcgat
     2221 cacccccttc cggcctgacc accggttcgg gcgccatgcc cgaccatgac tccatcattt
     2281 tttgcaccag gtgcgcgtgt ccggtagcca gttcctgctg aagtgaagcg agcggttcga
     2341 gatttggcat cggcaacggt gccgaaggtg ccaaaggcgc cgaggccggc aacaggacag
     2401 ccaactgttg cttggtgacg aaatcgaggc aggcttgcgt caatgcctga ccgacctgga
     2461 aaaaattcag tgccgaggct gcggattggg cagattgctc tgtgttcatg gacatcgtcc
     2521 tttcatcaca gtttgtcgca gaaaaacaga gggtacggac gctgtttctt tcgtatgctc
     2581 tcaattctga gcataggtaa cgaggaaaac atattcaagc cggcgattcg gaggcttccg
     2641 tgtcctgata ggcattgcgt gacttattgg tcagaatcca gtctgaaacg aaaccgaatc
     2701 gcgagtgctt gaaagccgga tcgtcggtga gaactttctc cggtttccct ttcctaacgc
     2761 gtggactagg gtggtcatgc gactttgtca atgggcacag cacttaacaa tatgtagcga
     2821 aacgcccagt tcagcgaggt agcgcttatg aaatacgtaa atcattccaa tctctttctc
     2881 cccttactct ttctgcttct gatggtgatc gcgggtggcg ttgcggcgca aacggtggat
     2941 ggagccagga ttacatctac ttgccaggtt gagcgaccgt cggacagcgg gcacggggca
     3001 aggtcggagg atgcgccttc gggcagcggt ttgagcttct ctatcggtgg ttttgccctc
     3061 ggtggcagcc gcccccgttc gccatcctat gaaagcgaaa gtgaaaatac accgagagaa
     3121 gaaagccatt cgcatggcaa tgtcggggtc ggcttgtcca tcaatctcgg cgaactgttc
     3181 cgacatttgg gcaaacccga aataccgcaa caactcttcg aggatgggcc gcgatttgaa
     3241 agtgctttca gcatggcctg tattccggta cgcggatttg tccgcggcgg ttggcccttg
     3301 gtcattgatt atcaggccga ccccggtacc gtcgcgtcgg tggagattca cgtcgagggt
     3361 cgcgatccgg tggtgattcc cctgcgccga ggcagcgaac acaatctact caaattgagg
     3421 ctgcccgatc agttggggaa cgagcttagc cccgcattgt tgttggtgcg tgcggtgaag
     3481 gaccagcccg gcgctcctgt gctcggccga atcaatgtgt ttggtctggg ggcaggtccc
     3541 cgggcagtag gttcggtagc catcgaccag gtcgacttcc agccgggaac gatgagtctc
     3601 ggcaagaagg aaaccgctcg attcagtttt tattcccgct ccgatttcaa ccgggcggcc
     3661 gctgaagtat tgcgtgtgat caaccgcgac ggcgacctgc aggttcgcct ggcccgttcc
     3721 attccattcg acggcggggt ggctgcggga acctgggtgg gccgtgacaa acccctgatc
     3781 tgggacggaa ccgatgccga ccggcaacct tcactgggcc agcatctgat gcaggttcgc
     3841 gcctggctga gcgcgcaggc cgggggcgac tgggttgcag cgtggtcggc ctggacggtt
     3901 gaagtcacgc gctgacgcga tgttcgcgtc catattgcgc taccgctggc tgcgggaact
     3961 gcgggccctc tggccgtggg ccgtggggct gctactgctt gccctggcag gccagctcgc
     4021 gctcacctcg ccgcgcacca gcgtcaatct cgattcgatc ggttcgctaa gagcattgcc
     4081 cggacaggcg attacgctga gtcacccggt ctcggacaga gtcctcgtta cttacacttc
     4141 gggccgtgaa ggcggccttg acctgcgttt cgagcaggcg cgtttggcgc cggaaacact
     4201 ggagcttctg cgtcgtgtgg ggatcatggc gccagccgtc gaagcgccag cgagctggat
     4261 tacccgtgcc gaaccgggga tcggagccga aagcatgtca agtttcgagg tggcggctgt
     4321 cgactctggc aagcttcccc gcacgttgct gctgcgcgtt ctggccggcg ccacccctcg
     4381 ccaggtgcgc ttggaattgc ttgcacgtgg cgcgccgctg gaagtcactc ttgcactgcc
     4441 gtggactggc gatgcacacg cgccgcgcaa gatgttgcgg cttggcgaga ccgagctttt
     4501 gctaccagga gccctgccaa taaagttgct ggttccgccg gaggcaagca tgcgggcaat
     4561 catcagtctg ccggacaagc cgcaagcctc acagtctgcc gaccttgaat tcggaacctt
     4621 cacgggtcgg gaaggcggcg gcctggcggt gtcagcactc ggtatagagg ccgctcctgg
     4681 cgcaggatcc cttctcgcgt gcggtgcccc ggcggggagc ctgctctgga gggctgcagg
     4741 gcgactggcc gctggcgatt gtcccgagag tgtcgggtcg atcagcgtac gttcgctgga
     4801 gctgcgggcg gacatggctg atctgacggt gggcggcaac gcctgggtgc ttaaggatgg
     4861 acagcccctg ggcgaagatc tccttagccg cctgcgccaa cagccggtct ggtgggcgct
     4921 gctcctgctc gttgatggat tgctcctttt gtgggcattg cttgccatcg tgccggggcc
     4981 gcgccgaatc accgtgcgtg gcgtattcat cagctaccgg cgagctgaca gcggtcccag
     5041 ggtagggcgg ctgcatgacc ggctggtggc acgtcttggt gccgaacggg tgttcctcga
     5101 tctggagtcg atcccggctg gagaagattt cgatcgtttc atcgccgacg gtctgtcccg
     5161 ggtcgatatc gttctggcgg tgatcggacc ccaatggctc gagttgcgag atgaacatgg
     5221 ccaacgccgg ctggatgccg aaagcgatct ggtacgccgc gagattgcgg ctgcactgct
     5281 tgccggagta agggtgatcc cggtgctggt tggcggggct ccgatgccga cagccgatca
     5341 gttgccgagc gacattgcgg ctctcgccgg acgaaatgcc atgaccatcg gcgatctcaa
     5401 gtttctgcgc gatgccgacg atctgatcga tcaattcgag tacgccccgg tgcattccac
     5461 ggggaatggc acgccctgat attgattcat ttcagcgggg gttcttccag agtttgctcg
     5521 atgtgcagcg tttgtgtcgg gaaggcgatc tcggcacgat gacgggcgat gattccggct
     5581 atccgcagga gaacatcctg ttttatttcg tgataatgca cccactgggt ggtattggtg
     5641 aaggtgtaga taaagaagtt aagcgacgaa tccgcaaatt tgtcaaagtt gacgatgagc
     5701 gtctgcttgg cgtcgatctc cgggtgctgc tcaagcatgg ctcttacatc ggcaacgatg
     5761 gcagccattt tgtcgatatc cgcgtaacgg atgccgacgg tttccttgat gcggcgatgg
     5821 ctcatgcgcg aaggattttc gaccacgatg gtagtgaaca cggcgttcgg tacgtaaatc
     5881 ggatttttgt tgaaggcccg aatgcgggtg tggcgccaac tgatcacctc gacggtgcct
     5941 tcgatctgtt tgtcggggga gcgaatccag tcgccgacgc tgaatggacg gtccagatag
     6001 atcgtcagac cgccaaagaa gttggcgatg atgtccttcg ctgcgaagcc gacggcgata
     6061 ccgccgatac cgccggccgc gagaataccg gcaacgctga atcccagcgt ctgcatgatg
     6121 accagtgccg cagtaaggac gaccgtcagg cggccaagct tggagaatgc gtcgacggtg
     6181 gtcttgtcga cttcatcgcc ctcacgcacc tttcgcgaaa cgtaatgcgc gctggcgaga
     6241 tgaatcatgc ggaacaggaa ccacgcaaag caggtcacta cgccaacgtc tcgaagcggg
     6301 gggaacaggt cgaacaacgg gtcgtcggca tgacggccta cgatcccagc agcgaaagcg
     6361 atgccgacca cccagattac ggcgggtaac ggacgccgag ccgcgtggaa aagaatatcg
     6421 ttccacgcat ctgtcgaatc cttcggtttt cgagtcgcct ttcgcagcag ccagttggca
     6481 aaaaagttca gcgccaccgt ggcgacgatc gcgatcaacg ctttcgccag ccagggttcc
     6541 gccaaaaaag aaaaaccgaa acgttcagcg agctctttca tcggaaatct ccatgtatgc
     6601 tcctccacga gcgatggcat tacatgttaa cccggtaagg tgcaatcacg cataggtgag
     6661 ccgctgacaa cgggcgacgc gacccgctcg acgtttgacg ctggttgaaa gggtctggca
     6721 gaaaaagcac acgaccagca tacggccggg caacggcttg acaggagcct tgagcgcgtc
     6781 tgtccagaaa tgacttgagg caagcccttg ttttgagcta gaatcctcga ccttcatccc
     6841 aagcacaact ggagcaacaa tcatggcgcg agtctgccaa gt
//
LOCUS       DAORPM010000120         6853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2891_length_6853_cov_7.784201, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000120 DAORPM010000000
VERSION     DAORPM010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2891_length_6853_cov_7.784201"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="PLD53_07105"
     CDS             <1..1676
                     /locus_tag="PLD53_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRC60717.1"
                     /translation="MQHIPGPDYPGGGQIISSAAEIAGAYSTGRGSLRVRACYEFEEM
                     ARGAWQLVIRELPPGVSSARVLSEIGALMNPQPKPGKKSIEQSAAQLKALLASQLERA
                     RDESGKDDDIRLVFEPISSRLGRDEFVALLLAHTSLETTAPINLVAVGIDGRPCQKTL
                     ADLIGEWCRFRLQTVRRRTEFRLAKANDRIHILEGRHIVFLNIDEVIRIIRESDEPKP
                     ALIARFALSDRQAEDILEIRLRQLARLEGIKIEQELAKLRDEKEGLLKLLDSDTLLRK
                     LVVREIKADASKHGDERRTLIQPAATSSRSEVATVADEPATLIVSEKGWARVRQGHGL
                     DLSGVVFKDGDGPGSTHECRSVDSLVIISSEGRAFTVPISSLPDGRGMGAPLPSFIEI
                     GAGKIAHVLIGKPEDELLVAKSSGYGFVCTFGDLLSRQKAGKAFLSMEVGATVLPPAP
                     IVRNAGMDHIAALSSDGRLLVFPLDQMKRLSGGKGVQIIGLKGKETLKSIIAGKGPSV
                     SIKGTFRNKLKTLLSEEKHVGQRARRGATVGLVNQPEIAYPKAEEPGAK"
     gene            1742..2281
                     /locus_tag="PLD53_07110"
     CDS             1742..2281
                     /locus_tag="PLD53_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60718.1"
                     /translation="MSSRVLLATLLALCSTLATAQGTVYESKDRAGPVFSDAPSSGAK
                     SLELPPLSVIQEPQAPPQPPPAQTVAPAPYSGLAIVAPANEGTIHTNTGAFDLHVQIQ
                     PALRISDGDAIIVRLDGNQLARGYHDTAIHIKAADWARAATAESIEHSLQVAITDRTG
                     AVLIESAPVRFYAHRAVRR"
     gene            complement(2424..3227)
                     /locus_tag="PLD53_07115"
     CDS             complement(2424..3227)
                     /locus_tag="PLD53_07115"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HRC60719.1"
                     /translation="MNKPKHLSLRGLGICLSVSAIVLFSGCATTSGSNVTVSAPTLMT
                     TAGFLTDYARLKTNPDIGGTECWRAPAIDASKYDKVLISRILVSLAPAKKEDSPKAID
                     PSDLTLLTDYFHKALTKNLEKQMTVVDKAGPGVLVIRIALTDLVPTQVSRSVAGTLIP
                     YGFVAEAGSGVAAGLPAGSTPYLGETGMEMQFRDGASNTVLAECRDTQIGRKYAADVD
                     AGASGATETWVDGYMNSFQSWSYAKNAMDKWSLLLATRLAHLRGVTPTK"
     gene            3442..>6853
                     /gene="purL"
                     /locus_tag="PLD53_07120"
                     /gene_synonym="purI"
     CDS             3442..>6853
                     /gene="purL"
                     /locus_tag="PLD53_07120"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRC60720.1"
                     /translation="MADILFLRGAPAFSAFRLLGLQQRFQAVAPGSSIASAEYWHIAK
                     LNKALSDAGRKQLAALFEARAENEAGGVECGELFLVTPRIGTISPWSSKATDIAWNCG
                     LEAVERIERVERGIAYRIKTANGTLSADERTQISTAIHDRMTETVLGDFEQTAELFRH
                     FPPTPMTSIDLLGGGRNALVEANGRLGLALSDDEIDYLVELFVNKTQRNPTDVELMMF
                     AQANSEHCRHKIFNASWVIDGETHNETLFGMIRETHQAHPQGTVVAYSDNSSVFEGAR
                     VARFCPGADGAYGWTEELTHILAKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGS
                     KPKAGLCGFSVSNLYLPDAPRPWEAEMASYGRPSRIASALDIMLEGPIGAAAFNNEFG
                     RPNLCGYFRSYEQRVGTGEEAVVRGYHKPIMVAGGIGNIAADQSFKAATFPPGTLLIQ
                     LGGPGMLIGLGGGAASSMSTGSNSESLDFASVQRGNPEMQRRAQEVLDRCWQMGQPRE
                     TAKDNASTPGNGNPILSVHDVGAGGISNAFPELAHGAGCGAHFELRDVKIEEPGMSPA
                     EIWSNEAQERYVLAIPPSRLPEFTTICERERCPFAVVGIATDDNLLRVSDRHFGDLPV
                     DMDMDALLGKPPRMTRDVDHLLSLEVAFDPSSLELKDAAYRVLHLPAVADKTFLISIG
                     DRSVGGLTARDQMVGPWQVPVADVAVTTMGYRTNLGEAFAMGERTPLAILSAPASGRM
                     AVGEALTNIAAANISGLGDVKLSANWMAAAGSPGEDASLFDTVRAVSDLCQQIGIAIP
                     VGKDSLSMRTAWDDEGLRKQVVSPLSLVVTAFAPVQDARRTLTPQLCLDVGETDLLLI
                     DLGAGRNRLGGSALAQVFNATGSEAPDVDVPEKLLAFFGAVQTLASDNLLLAYHDRSD
                     GGLFATVAEMAFAAHCGVSLDMDGLCYDPLTNDIDGAEKKPDLLRGRAFELMMRALFN
                     EELGAVLQIRRTERAQVMGVLRAAGLGACTHFIGSLNPWDEFRVIRNAKAVLREKRVD
                     LQRAWSETSYRMQALRDNPECAQQEYDRILDATDAGLSASLTFDPAEDIAAPFINSGT
                     RPKIAILREQGVNGHVEMAAAFDRAGFASIDVHMSDILSGRVSLAGFKG"
BASE COUNT         1379 a         2146 c         2024 g         1304 t
ORIGIN      
        1 tgatgcagca catccccggg ccggattatc ccggtggcgg ccagatcatt tcttccgccg
       61 cggaaatcgc cggcgcctac agcacgggtc gcggcagcct gcgcgttcgc gcctgctacg
      121 agttcgagga aatggcgcga ggcgcctggc aactggtgat ccgggaactg ccgcccggcg
      181 tttcctcggc ccgcgtgctc tccgaaatcg gcgcgctgat gaacccgcag ccgaagccgg
      241 gcaagaaatc gatcgaacaa tccgccgctc agttgaaagc ccttctggcc tcgcagctcg
      301 aacgcgcgcg cgacgaatcg ggcaaggacg acgacatccg cctcgtcttc gagccgatca
      361 gctcgcgact cggccgggat gaatttgtcg ccctgttgct ggcccatacc agccttgaaa
      421 ccaccgcacc gatcaacctg gtcgccgtcg gcatcgacgg ccggccatgc cagaaaacac
      481 tggcggacct gatcggcgaa tggtgccggt tccgcttgca gaccgtacga cggcgcaccg
      541 aattccgcct cgccaaggcc aacgaccgca tccacatcct ggaaggccgg catatcgtct
      601 ttctcaacat cgacgaggtg atccgcatca tccgcgaatc ggacgaaccc aagccggcgc
      661 tcatcgcacg tttcgcgctc tccgatcgcc aggctgaaga catcctcgaa atccgtctgc
      721 gccagttggc tcgactcgaa ggcatcaaaa tcgagcagga acttgcgaag ttgcgcgacg
      781 aaaaagaagg gctgctcaag ctgctcgaca gcgacacctt gctgcgcaag ctcgtcgtgc
      841 gcgagatcaa ggctgacgca agcaaacacg gcgacgagcg ccgcacgctg atccagccgg
      901 cagcgacgtc ctcgcgctcg gaagtggcca cggtcgccga cgaaccggcg acactgatcg
      961 tttccgaaaa aggctgggcg cgcgtccgcc aggggcatgg actcgacctc tccggagtcg
     1021 tcttcaagga cggcgacggc ccggggtcga cgcacgaatg ccgctcggtc gactcgctgg
     1081 tcattatctc gagcgaaggc cgcgccttca ccgtgccgat ttccagcctc cccgatgggc
     1141 gcggcatggg ggcgccgtta ccttccttca tcgaaatcgg cgccggcaag atcgcgcacg
     1201 tgctgatcgg caaaccggaa gacgaactgc tggtggccaa atcctccggg tatggcttcg
     1261 tctgcacctt cggcgacctg ctctcgcgac agaaggccgg caaagccttt ctgtccatgg
     1321 aagtcggcgc gactgttctg ccgcccgccc caatcgtacg caacgctggc atggaccata
     1381 tcgccgccct ctccagcgac ggccgccttc ttgtattccc gctcgaccag atgaagcgcc
     1441 tgtccggcgg caagggcgtg cagatcatcg gactgaaagg caaggaaacg ctcaagtcga
     1501 tcatcgccgg taaaggcccc tccgtcagca tcaagggaac gttccggaac aagctgaaga
     1561 cactgctttc ggaggagaag catgttggac agcgtgcgcg aagaggagcg accgtggggc
     1621 tagtgaatca gccggagatc gcgtatccga aggcggagga accgggagcg aagtaaacgg
     1681 atattcggct gtagagtaat gatgcataat caatactcat gaacagacaa aggaggcgac
     1741 tatgtcatcc cgtgttttgc tggcaacctt gcttgcactc tgctccacgc tggcgacggc
     1801 tcaagggaca gtctacgaat cgaaggaccg ggccgggcct gtattttcgg acgcaccttc
     1861 ttccggtgcc aaatcacttg aactgccacc actgagcgtc attcaggaac cccaggcgcc
     1921 gccacaaccg ccgccggcgc aaactgtcgc acctgcgcca tacagcggcc tggccatcgt
     1981 cgctccagcg aacgagggca cgatccatac gaacacgggt gcattcgacc tgcacgtaca
     2041 gatacaaccc gcgctaagaa tcagcgacgg cgatgcaatc atcgtaagac tcgacggaaa
     2101 ccagcttgcg cggggatatc acgacacggc gatccatatc aaggcggccg attgggcgcg
     2161 tgcagccacc gcagagagta tcgagcacag tttgcaggtg gcgattaccg atcgaaccgg
     2221 agccgtgctg atcgagtctg ctcctgtcag gttctacgca caccgcgccg tacggcgcta
     2281 gcacgctgct ccacatgccc ggtagccagg acaaggcgcc acctgctact tttgattttc
     2341 tccaaccggc aaccatatct gctggctgtc ggcaccgtct gacccacggg tcagacgggt
     2401 tcccgcaatc agcacaacgc aacctatttg gtcggggtaa ccccacgcaa atgggcgagg
     2461 cgcgtcgcta acagcaatga ccacttgtcc atcgcattct tggcatacga ccaggattgg
     2521 aaggagttca tataaccatc cacccatgtc tcggttgcgc ccgacgcgcc cgcatcaaca
     2581 tcagccgcat atttgcgacc aatttgcgta tctctgcatt cagcaagcac ggtgttggag
     2641 gccccatcac ggaactgcat ttccataccc gtttcgccca gatacggtgt tgaacccgcc
     2701 gggaggccgg cggcgacgcc tgaacctgcc tcagccacaa agccataggg aatcagcgta
     2761 ccagctacac ttcggctcac ttgagtcggc accagatcgg tcagcgctat gcgaatcacc
     2821 agcacacccg gaccggcctt gtcgacgacg gtcatttgtt tttccaagtt cttggtcagc
     2881 gctttgtgaa aatagtccgt tagcaacgtg agatcactcg ggtcaatcgc tttcgggcta
     2941 tcttctttct tggccggtgc caaagagaca agaattcgtg aaatcagcac cttgtcgtac
     3001 ttgctggcat caatcgctgg ggcacgccag cactcggttc cgccaatatc cgggttggtc
     3061 ttcaggcggg cgtaatcagt caaaaacccg gccgtcgtca tgagtgtggg cgcactgacg
     3121 gttacgttcg aacccgacgt tgtggcacag ccggagaaca atacaattgc gctaacggaa
     3181 aggcatatgc ccagaccacg aagagaaaga tgcttcggct tattcattac tcactactcc
     3241 tatgttcgcc cgttaacagt ttaacgaagc agtcgaaaac gcgcattctg acacaattcg
     3301 agtctgagaa gcccccgtac ttatcgcacc aatacaccga ttccattgtc gttgccggtc
     3361 cgactgcaag ccgctctcgg ctataatccc ggcttcgcct tgtatccctc gcaggcttcc
     3421 agcccttatt caaaactcac catggccgac attctatttc tgcgcggagc ccccgctttt
     3481 tccgcttttc gcctgctggg tttgcagcaa cgctttcaag ccgtcgcgcc tggttcgagc
     3541 atcgccagcg ccgaatactg gcacattgcc aagttgaaca aagcgctcag cgatgcaggc
     3601 cgcaaacaat tggcggcgct gttcgaggcg cgcgcggaga atgaagccgg gggtgttgag
     3661 tgtggagaac tattcctcgt caccccgcgc atcggcacca tctcgccgtg gtcgtccaag
     3721 gcgaccgaca tcgcgtggaa ctgtggcctg gaagccgtcg agcgaatcga acgcgtcgag
     3781 cgcggcattg cctatcgcat caagaccgcc aatggaacgc tgtcagccga cgagaggaca
     3841 cagatttcaa cggccatcca tgaccgcatg accgagaccg tactcggcga tttcgagcaa
     3901 acggctgagc ttttccgtca cttcccgccc acaccgatga cttcgatcga tttgctgggc
     3961 ggcggacgca acgctctggt cgaagccaac ggccgcctcg ggctggcgct gtcggacgac
     4021 gagatcgact atctcgtcga gttgtttgtg aacaagaccc agcgcaatcc gaccgacgtc
     4081 gagctgatga tgttcgcgca agccaactcc gaacactgcc gccacaagat attcaacgcc
     4141 tcgtgggtca tcgatggcga aacgcacaat gaaacgctgt tcggcatgat ccgcgagacg
     4201 caccaggcac atccgcaagg cacggtcgtc gcctactccg acaactcctc ggtgttcgag
     4261 ggcgcacgcg tagcacgctt ttgtccggga gcggacggcg cctatggctg gaccgaggag
     4321 ctgacgcaca ttctggccaa agttgaaacg cataaccatc cgactgcgat ttcgcccttc
     4381 cccggtgccg cgaccgggtc gggcggcgag attcgcgacg aaggggcgac cgggcgcggt
     4441 tcgaagccga aggccggtct ctgcgggttt tcggtctcca atctgtatct gccggatgcg
     4501 ccgcgaccgt gggaagccga aatggcctcg tatggccgcc cgtcacgcat tgcctcggca
     4561 ctggacatca tgctcgaagg cccgattggc gcagcggcct tcaacaacga atttggccgc
     4621 ccgaatctct gcggttactt tcgcagttat gagcaacgtg tcggtacggg tgaagaggct
     4681 gtcgtacgcg gttaccacaa gccgatcatg gttgccggtg gcatcggcaa cattgctgcc
     4741 gatcagtcct tcaaggctgc gacgttcccg ccgggcaccc tgctcatcca gcttggcggg
     4801 ccaggcatgc tgatcggact gggtggtggc gcggcgagtt cgatgagcac cggcagcaat
     4861 agcgaatccc tcgactttgc gtcggtgcag cgcggcaacc ccgaaatgca gcgacgggcg
     4921 caagaagtgc tcgaccgttg ctggcaaatg ggacagcccc gtgaaacggc gaaggataat
     4981 gcttcgacgc ccggtaacgg caatccgatt ctctcggtgc atgacgttgg cgcgggcggc
     5041 atctccaacg cctttcccga gttggcgcac ggcgccggtt gcggcgcgca ctttgaattg
     5101 cgcgacgtga agattgagga gcccggcatg tcgccggccg aaatctggag caacgaggcg
     5161 caggagcgtt acgtgctggc gatcccgccg agcaggctgc ccgaattcac caccatctgc
     5221 gaacgcgagc gttgcccgtt tgccgtagtt ggcatagcca ccgacgataa tctcctgcgt
     5281 gtaagcgacc gtcatttcgg cgacctcccg gtcgatatgg acatggacgc attgctcggc
     5341 aaaccgccgc gcatgacgcg cgatgtggat catctgctgt cgctggaggt ggcgtttgat
     5401 ccatcctcgc tcgaactgaa ggatgccgcc tatcgcgtgc tgcatctgcc agcggtggcc
     5461 gacaagacct tcctgatatc aattggcgac cgctcggttg gcgggctgac ggcgcgcgac
     5521 cagatggtcg gcccctggca ggtgccggtt gccgacgtgg ctgtgacgac catgggctac
     5581 cggaccaatc ttggcgaagc ctttgccatg ggtgagcgta cgccgctggc gatcctcagc
     5641 gctccggctt cgggccgcat ggcggtaggc gaggcgctga ccaacattgc tgcggccaat
     5701 atctccggtc tgggcgacgt gaagctctcg gccaactgga tggcggctgc aggttcgccg
     5761 ggagaggatg ccagtctgtt cgatacggta cgcgccgtct ccgatctctg tcagcagatc
     5821 ggcattgcga ttcccgtcgg caaggattcg ctgtcgatgc gcacggcttg ggatgatgag
     5881 gggctgagga agcaggttgt ctcgccactg tcgctggtcg tcactgcgtt tgcgccggtg
     5941 caggatgccc gccgaaccct gacaccacaa ctctgtctcg acgtcggaga aaccgatctg
     6001 ctgttgatcg accttggcgc cggcaggaat cgtcttggtg gttcggcgct ggcgcaggtg
     6061 ttcaacgcca ccggctcgga ggcaccggac gtcgacgtcc cggaaaagct attggcattt
     6121 ttcggagcgg tacagacgct cgccagcgac aatcttttgc tcgcctatca cgaccgatcg
     6181 gatggcggcc tgttcgccac tgtcgccgaa atggccttcg ccgcgcattg cggcgtatca
     6241 ctggatatgg atggcctgtg ttacgacccg ctgaccaacg atatcgacgg tgccgagaag
     6301 aaaccggact tgctccgcgg tcgcgctttc gagttgatga tgcgcgcact gttcaatgag
     6361 gaactcggtg ccgtgctgca gatccgtcgc actgagcgcg cacaggtgat gggcgtgctg
     6421 cgcgctgccg gcctcggggc gtgcacccat ttcatcggct cgctcaatcc gtgggacgaa
     6481 ttccgcgtca tccgcaatgc caaagccgtg ctgcgcgaaa agcgcgtcga tttgcagcgc
     6541 gcctggtcgg agacaagcta ccgcatgcag gcgctgcgcg acaacccgga atgcgcgcag
     6601 caggaatacg accgcattct cgatgctacc gatgccggcc tttcggcttc gctgaccttc
     6661 gaccctgctg aagatatagc cgctccgttc atcaacagcg gcactcgccc caagattgca
     6721 atcctgcgcg aacaaggcgt caacggccat gtggaaatgg ccgctgcatt cgatcgcgcc
     6781 ggctttgcct cgattgatgt gcatatgagc gacattctct cgggccgtgt atcgctcgcc
     6841 gggttcaagg gaa
//
LOCUS       DAORPM010000121         6827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2910_length_6827_cov_10.410662, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000121 DAORPM010000000
VERSION     DAORPM010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2910_length_6827_cov_10.410662"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLD53_07125"
     CDS             complement(<1..361)
                     /locus_tag="PLD53_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRC60721.1"
                     /translation="MSNKFVYVFGASKTEGSADMKNLLGGKGANLAEMCRLGISVPPG
                     LTISTEACTVYTEKGAESIQKLLDAEVRQGVAFLEKEMGKKFGDAADPLLVSVRSGSR
                     ASMPGMMDTILNLGLNDE"
     gene            complement(649..1128)
                     /gene="ispF"
                     /locus_tag="PLD53_07130"
     CDS             complement(649..1128)
                     /gene="ispF"
                     /locus_tag="PLD53_07130"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRC60722.1"
                     /translation="MIRVGQGCDIHALVLGRKLIIGGVEIPYEYGLLGHSDADVLLHA
                     IIDALLGAAGLGDIGRHFPDSDERYRGVDSRVLLRCTGELLAQAGWRIVNVDSTINVQ
                     RPKMWPHLPQMVSNIASDLLLPEGRVNIKAKTAERLGFVGRGEGVSADAVVLIELNE"
     gene            complement(1125..1814)
                     /gene="ispD"
                     /locus_tag="PLD53_07135"
     CDS             complement(1125..1814)
                     /gene="ispD"
                     /locus_tag="PLD53_07135"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRC60723.1"
                     /translation="MPCHYAIVPAAGSGSRFGSEVPKQYLQIDGQPLIHHSLAALCHC
                     DRIERVWVVLAPEDEWWGLYDWSSLGSKLQAVFCGGATRAESVLNGLATAARILADDD
                     WVLVHDAARPCLSQTMIAALCDELADDPIGGILAVPVADTLKRADVDQRVADTESRDG
                     LWLAQTPQMFRYGVLRKALAENITVTDEAGAVEAAGLRPRLVRADATNLKVTFPADIQ
                     LAKLILQDRNS"
     gene            complement(1934..2443)
                     /locus_tag="PLD53_07140"
     CDS             complement(1934..2443)
                     /locus_tag="PLD53_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60724.1"
                     /translation="MNISGVLISSTVVVLLVGCASAPTGPSVLSLPGTGKSFGDFRLD
                     DAQCRQYAAQQTGALPSDPGVRNAVIATAVGALAGAAINGSQGAGIGAGAGLVVGSVS
                     GAGEARGATYSSQHQYDNAYIQCMYASGNKVPVPAAMVNSQSRVPVAPSPGRYYPPPP
                     PDYYPPPAR"
     gene            complement(2465..2851)
                     /locus_tag="PLD53_07145"
     CDS             complement(2465..2851)
                     /locus_tag="PLD53_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60725.1"
                     /translation="MKEIESVKRGIVLLLLVLTGIGSAWADRGYGHRYGHGYGHRSAQ
                     FGFVITPGWGPWYYPPPFYYPPTQPIVIERAPPVYIEQVLPAPGPPETQTNYWYYCGA
                     TRAYYPYVNECPGGWQKVLPRPPGQP"
     gene            complement(2948..3856)
                     /locus_tag="PLD53_07150"
     CDS             complement(2948..3856)
                     /locus_tag="PLD53_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC60726.1"
                     /translation="MGGADATARSDAQREKRTAILSLLTGALVWGVIWYPYRILRDAG
                     IDGVVALAITYGVAFVSGLFFFRRELASFSPSWTLLWLALASAGCNLGYVLATLNGEV
                     VRVLLLFYLAPLWTVLLSHFLLGERLNRFGAFVISLSLAGAATMLWHPRIGLPVPQDA
                     GDWFGLGAGFSFALLNVLSRRAQGVSIEIKSMATFAGAVLVGMALVLAGFAQPQMPDS
                     PTIWLLLGLIGCVLLVVNLVVQYGLARVAANRAIVIMLSEVGFAALASWLLAGEVIGH
                     REWVGGAMIVAASVFSAKMERIDCEA"
     assembly_gap    3901..4000
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4087..5292
                     /gene="dapC"
                     /locus_tag="PLD53_07155"
     CDS             4087..5292
                     /gene="dapC"
                     /locus_tag="PLD53_07155"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HRC60727.1"
                     /translation="MNPNLARLHPYPFERLRQLFSGMTPNPKLREIKLSIGEPQHATP
                     AFIKDALTAGLGGLSNYPTTLGIPALRHAVAEWMERRYGLPDIDPEAHIIPVNGSREA
                     LFAFAQTVIDPSGSSNRSYIPLVVSPNPFYQIYEGAAYLAGADLRFLNTLPENNFAFD
                     YDTLSDEEWQRVQLLFVCSPGNPTGKTLDLEDWKLLFALSDKYGFIIASDECYSEIFF
                     DEANPPLGCLQAARTLGRSFDRLVMFSSLSKRSNVPGMRSGFVAGDAAILKQFLLYRT
                     YHGSAMNPAVQAASIAAWNDESHVVANRDLYREKFAAVTPLLTRVLGTAMPDASFYLW
                     ARTPLADTEFARALAADYNVVVLPGSFLARQSCGVNPGAGFVRIALVAPLAECLEAAD
                     RIREFVSKL"
     gene            5302..6123
                     /gene="dapD"
                     /locus_tag="PLD53_07160"
     CDS             5302..6123
                     /gene="dapD"
                     /locus_tag="PLD53_07160"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRC60728.1"
                     /translation="MQQFQQTIEEAWEDRTSLQPGSAPAKVGEAVSAALTELDEGRLR
                     VAEKVNGEWITHQWLKKAVLLSFRLEDNQLIDGGPMRYFDKVPTKFARYDAKAFERGG
                     FRVVPPATARRGAFIARNVVLMPSYVNIGAYVDEGTMVDTWSTVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVGEGSVISMGVYIGQSTKIFDRE
                     TGEVSYGRIPPGSVVVSGNLPATDGSYSLYCAVIVKKVDAKTRAKTSINDLLRGD"
     gene            6184..>6827
                     /locus_tag="PLD53_07165"
     CDS             6184..>6827
                     /locus_tag="PLD53_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRC60729.1"
                     /translation="MILDKLFQLMAEKQASDIFVSAGVPIHIKIQGNSVPVNQQVMDS
                     AMIEKIAFELMSPDQLRTFEATMEMNLSFGVPNVGNFRVNIFRQRASISIVVRYILGN
                     IPSLDSLMLPSVLGELIMEKRGLILIVGSTGSGKSTTIASMLDYRNANRSGHILTIED
                     PIEFLFRHKKSIVNQRELGMDTLGWEPALKNAMRQAPDCILIGEIRDKETMQAA"
BASE COUNT         1551 a         2055 c         1771 g         1350 t
ORIGIN      
        1 cctcgtcatt gagacccaga ttgagaatcg tgtccatcat gccgggcatc gaggcacgcg
       61 atccggagcg caccgagacc agcaaggggt cggcggcatc gccgaatttc ttgcccattt
      121 ccttttcgag gaaggctacg ccctgcctta cctcggcgtc gagcagcttt tggatgctct
      181 cggcaccctt ttcggtatag accgtgcaag cttcggtact gatcgtgagg cctggtggca
      241 ccgagattcc caagcgacac atttctgcga gattggcacc tttgccaccc agcagattct
      301 tcatatcggc cgagccttcg gtcttcgaag cgccaaaaac gtaaacgaac ttattcgaca
      361 tattgacctc actaaaacgg aaaaattaaa aagtacgacg tgaacggtcc acgcattttt
      421 gaaatattta tctgaccgaa cggagagagt tgatagttta ttaggacaaa ccttacgctc
      481 ttgacccgca actataccaa ggatcaagga gtcgtcccaa ttttgttgac ttcggcaagc
      541 gacccaaaag cctttacctg aaaattactg aattctgagc aaacaaaccg aatccattga
      601 aaacccgtcg tgacccaaga acaatggccc ataaagaagt gggaaccttc attcattgag
      661 ctcgatcaag accactgcgt ctgcagaaac cccttccccg cgcccgacaa accccaagcg
      721 ttcggcagtc ttcgccttga tgttaacccg gccctcaggt agcagcaaat cactggcaat
      781 gttgctaacc atctgcggaa gatgtggcca catcttcggg cgctgcacat tgatcgtcga
      841 atcgacgttg acaatgcgcc agccggcctg ggcgagcaat tccccggtgc aacgcaatag
      901 cactcggctg tcaacaccgc gatagcgctc gtcgctatcg gggaaatgcc gaccgatatc
      961 gcccagcccc gccgcgccaa gcaacgcatc gatgattgcg tgcagcagca cgtcagcatc
     1021 ggaatggccg agcagcccgt actcataggg aatctccacg ccgccaatga tcagcttccg
     1081 gccgaggacc agcgcatgaa tgtcgcaccc ctggccaacg cgaatcatga gttcctatcc
     1141 tgaaggataa gcttcgccag ctgaatgtcg gcaggaaaag taaccttgag attggtcgcg
     1201 tcggcacgca ccaaccgtgg cctgagaccg gcagcttcga ctgcacctgc ctcatcggtt
     1261 accgtgatgt tttcggccag agccttgcgc agtacaccgt agcgaaacat ctgcggtgtt
     1321 tgcgccagcc acagcccgtc tcgcgactcg gtatccgcga cacgctgatc cacatcggcg
     1381 cgcttcaagg tgtcggcaac aggaacggcc aggatgcctc caatcggatc gtcggccagt
     1441 tcgtcgcaaa gagcggcgat catggtctgc gacaggcaag gacgtgcggc gtcatgcacc
     1501 agcacccagt catcgtcagc caatattcga gcggcagtcg caaggccatt caacacgctt
     1561 tcggcgcgag tggccccgcc acagaaaacg gcctgcaatt tcgagccgag gctactccag
     1621 tcatacaacc cccaccactc atcctccggt gcaagtacaa cccagacgcg ctcaatccgg
     1681 tcgcaatgac acagggcagc gagtgagtga tggatcagtg gctgcccgtc aatctgcaga
     1741 tactgtttcg gcacttccga tccgaagcgc gaaccgctgc cggcagcggg aacaatggcg
     1801 taatggcaag gcatggcgcc attctagcgg aaacgcttgc aacgcgagct gattcaggaa
     1861 agacagaaca atcgagaaga gtgcgcttca caaaccaaca aagatgcatc gtcgtgaaac
     1921 ctctacggac gattcaacgc gctggcggcg gataataatc cggaggcgga ggcgggtagt
     1981 atctgcccgg cgacggtgct accggcaccc gcgactgact gttgaccatc gccgcgggaa
     2041 cggggacctt gtttccgctg gcatacatgc actggatgta ggcattgtca tactgatgct
     2101 gcgaactgta cgtagcacca cgcgcttctc ctgcccctga cacactcccg acgaccagtc
     2161 cggcacccgc tccaatgcca gcaccctgac tcccattgat cgccgcaccc gccaaggcac
     2221 ctacggccgt tgcgatgact gcattgcgaa ctcccgggtc gctcggcaga gcacctgtct
     2281 gttgcgcggc atactgacgg cactgcgcat catccaggcg aaagtcgccg aaactcttcc
     2341 cggttccagg caaagacaga acactcggac ctgttggtgc gctggcgcac ccaactagca
     2401 gcaccacaac cgttgaggag atcaatacac cggaaatatt catgtcaatg ctttctttgc
     2461 agcatcaagg ttgtcctggg gggcgcggca acaccttctg ccagccaccg gggcattcat
     2521 tcacataggg atagtacgct cttgttgcgc cacagtaata ccagtagttg gtttgtgttt
     2581 ctggtggccc aggcgcaggc aatacctgct cgatgtagac gggcggagca cgttcaatca
     2641 ctatcggctg ggtcggtgga tagtagaagg gaggtgggta ataccagggg ccccagccgg
     2701 gagtgatcac gaaaccgaat tgggcagatc gatgaccata cccatggcca tatcggtggc
     2761 catagccgcg atccgcccag gcactaccaa tacctgtcag gacaagcagc aacagtacga
     2821 tgcctcgttt gaccgattcg atttctttca tcttgcctgc tccagtattc attcgccccg
     2881 aattctttct ggggcattac acaaatcaac gggcaacttc catccgggtt gacgacagcc
     2941 aaaaacctca ggcttcacag tctattctct ccatctttgc tgagaataca cttgcggcga
     3001 cgatcatcgc gcctcctacc cattcgcgat ggccgatcac ctcgccggca agcaaccagg
     3061 aagccagcgc cgcaaagcca acctctgaaa gcatgatgac aattgcccgg ttggcggcga
     3121 ctcgtgcaag cccatactga acaacgagat tgacaacgag caatacgcaa ccgatcaggc
     3181 cgagcagtag ccagatcgtg ggtgagtccg gcatttgcgg ctgagcaaat ccggcaagaa
     3241 caagcgccat accgaccagc accgctccgg caaaggtcgc catcgatttg atttcgatgc
     3301 ttacaccttg cgctcgcctg gaaagcacat tgagcagagc aaacgagaag cccgcaccca
     3361 atccgaacca atcgcccgca tcctgaggca cgggcagacc gatgcgcgga tgccagagca
     3421 tggttgctgc gcccgccaac gaaagcgaaa tgacgaaagc accaaagcga ttgaggcgct
     3481 cgccgagcag gaagtgcgaa agcagcacgg tccacaacgg ggcgaggtaa aacagcagaa
     3541 ggacgcgaac gacctcgcca ttcagcgtgg caagcacata gccaaggttg cagcctgccg
     3601 aggccagggc aagccagagc agcgtccatg agggggaaaa actggcaagc tcgcgcctga
     3661 agaagaacaa ccctgataca aaggccacac cgtaggtaat tgctagtgcc acaacgccgt
     3721 ctattccggc atcgcgcaga atgcggtagg gataccagat gaccccccag accagcgcac
     3781 ccgtaagcag cgagagaatg gccgtacgtt tctcgcgttg tgcatcgctc ctggctgttg
     3841 cgtctgcccc tcccattacc gtcaagcgat atcccctaat tcagtgatgc cccttataat
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcccctccca ttaccgtcaa
     4021 gcgatatccc ctaattcagt gatgcccctt ataattccct atcccatacg gcgtattgca
     4081 aataacgtga atccaaatct cgccagactg catccctacc cgtttgaaag actgcgccag
     4141 ctcttttccg gcatgacgcc gaaccccaaa ctgcgggaga tcaagctctc gattggcgag
     4201 ccgcaacacg cgacaccggc tttcatcaag gacgcgctga ctgccggact cggcggtctg
     4261 tcgaattatc ctaccacgct gggcataccc gccttgcgtc atgccgtcgc cgaatggatg
     4321 gagcgccgct atggccttcc cgacatcgat ccggaggcgc atatcatccc ggtcaacggc
     4381 tcgcgcgagg cgctgttcgc ctttgcacag acggttattg atccgtcggg gagcagcaac
     4441 cgaagttaca tccctctggt tgtgagcccc aatcccttct atcagattta cgagggtgcg
     4501 gcttacctcg ccggcgccga tctgcgcttt ctgaataccc tgccggaaaa caactttgct
     4561 ttcgattacg acaccctgag cgacgaagaa tggcaacggg ttcagctcct ttttgtttgc
     4621 tcgcccggca acccgaccgg gaaaacactc gatcttgaag actggaagct cctcttcgcc
     4681 ttgtctgaca aatatggctt tatcatcgcc tccgacgagt gttactcgga gattttcttt
     4741 gacgaggcga atcctccgct cggatgcctt caggcagcca ggacacttgg ccgctctttc
     4801 gaccgactgg tgatgttttc cagcctgtcg aaacgttcga atgttccggg catgcgctcc
     4861 ggcttcgttg ccggcgatgc ggcgatcctc aagcagtttc ttctctaccg tacctatcat
     4921 ggaagtgcga tgaatcccgc cgttcaggct gccagtatcg ccgcctggaa tgacgaatct
     4981 cacgttgtcg caaaccgcga tctttaccgg gagaagtttg cagccgttac accgctactg
     5041 acccgcgtgc tgggaacggc aatgcccgat gccagcttct acctgtgggc aaggacccca
     5101 ctggccgata ccgaattcgc ccgcgcgctg gccgccgatt ataatgtagt cgttctgccg
     5161 ggcagctttc tcgcccgtca gtcctgcggc gtcaatccgg gcgccggatt tgtgcgtatc
     5221 gcactggtcg caccgctcgc cgaatgcctc gaagccgcag atcgaatacg agaattcgtt
     5281 tcaaaacttt aggaaaaata catgcagcaa tttcagcaaa ccattgaaga agcctgggaa
     5341 gaccggactt cgttacaacc cggcagcgca cccgccaaag tgggtgaagc cgttagcgca
     5401 gcgctgaccg aactcgacga aggccggctt cgcgtggctg aaaaggtcaa cggcgaatgg
     5461 attacccatc agtggctcaa gaaggctgtt ctgctctctt tcaggcttga ggacaaccag
     5521 ctgatcgacg gtggcccgat gcgctatttc gacaaggtac cgaccaagtt cgcacgatac
     5581 gacgccaagg cattcgagcg cggcggtttc cgcgtggtgc caccggccac cgcccgacgc
     5641 ggcgccttta tcgccaggaa cgtcgtactg atgccctcct acgtgaatat cggtgcgtat
     5701 gtcgacgaag gaacgatggt cgatacgtgg tctaccgtcg gttcgtgcgc gcagatcggc
     5761 aagaacgtgc acctttccgg cggcgtcgga attggcggcg tactggagcc cttgcaggcc
     5821 aacccgacaa tcatcgaaga caactgcttc atcggcgccc gctcggaggt cgttgaaggc
     5881 gtaatcgtcg gcgaaggctc cgtgatctcg atgggcgtgt acatcggcca atcaacaaag
     5941 atcttcgatc gcgaaaccgg ggaagtcagc tatgggcgca tcccccccgg ctcggttgtt
     6001 gtttccggca acctgccggc aacagacggc agctacagtc tctattgtgc agtgatcgtc
     6061 aagaaggtcg acgccaagac acgcgccaag accagtatca acgacctctt gcgcggcgac
     6121 taactcctct tcgttccctc attctttttc acttttcgta ttcttgacaa cggcggaggc
     6181 cacatgattc tggacaaact attccagttg atggcagaaa agcaggcttc ggatattttt
     6241 gtctctgccg gcgtcccgat tcacatcaag atccagggca attcggttcc ggtcaatcaa
     6301 caggtcatgg actcggcgat gatcgaaaag atcgccttcg aactgatgtc gcctgaccag
     6361 ctccggacct tcgaagcgac gatggagatg aatctctcct ttggcgttcc caacgtaggc
     6421 aatttccggg tcaatatctt ccggcagcgc gcctcgatct cgatcgttgt gcgctatatc
     6481 ctcggcaaca ttccgtccct tgactccctc atgctgccct cggtgctggg tgagctgatc
     6541 atggagaaac gcggcctgat cctgattgtc ggctcaaccg gttcaggaaa gtcgacgacc
     6601 atcgcgtcca tgctcgacta ccgcaatgcc aaccgcagcg gtcacatcct gaccatcgag
     6661 gaccccatcg agtttctttt caggcacaag aaatcgatcg tcaatcagcg cgaattgggc
     6721 atggacaccc tgggttggga accggctctg aaaaacgcca tgcgccaggc ccccgactgc
     6781 atcctgatcg gcgaaatccg cgacaaggaa accatgcagg ccgccat
//
LOCUS       DAORPM010000122         6825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2912_length_6825_cov_13.553028, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000122 DAORPM010000000
VERSION     DAORPM010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_6825_cov_13.553028"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1824
                     /locus_tag="PLD53_07170"
     CDS             85..1824
                     /locus_tag="PLD53_07170"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HRC60730.1"
                     /translation="MYRYDSIDQQIINERVAQYRDQMDRHQAGVLSEDELRPLRLQNG
                     LYIQRHGPMLRIAIPYGMLSAAQLRKLAEISRRYDRSVGHFTTRQNMQLNWPKFEETP
                     EILGELASVEMHAVQTSGNCIRNITTDQFAGIAPDELTDPRPYCEVLRQWSTFHPEFA
                     FLPRKFKIAVNAAREDRAVIRAHDVGLEIVNNDAGEIGVRVFVGGGLGRTPVIGKVIR
                     EFLPRKHLLSYLDAVLRVYNRYGRRDNMYKARIKILVNALGVEEFARQVEADWSRMKD
                     GETTLTDAEFDRIAQHFSPPSYENLPADDFAHATRLKEDKAFASWAKRCVHAHKVPGY
                     AAVTLSLKAPGAAPGDITDLQMEAVADLADRFSFGELRVTHEQNVVLADVRKCDLYEV
                     WKIARANALATPNIGLLTDMICCPGGDLCALANARSVPIAEAVQSKFDDLDYLYDIGD
                     ISLNISGCMNSCGHHHVAHIGILGVDKNGEEWYQVTIGGQQGNETTIGKVIGPSFYAD
                     EVPLVIEALINVYTEQRTPNERFIDTLQRIGIEPFKLRAYAGRDKRKPANAAAAHIKA
                     DSANETLTKVANA"
     gene            1817..2326
                     /locus_tag="PLD53_07175"
     CDS             1817..2326
                     /locus_tag="PLD53_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HRC60731.1"
                     /translation="MPKIIKNGQVVADHRQLLKLDGDQTPASLTLPDGDIMVPLAVWL
                     ARQEEILSRDNSPGVWLDSHEDVEAIAGDLEHFAVIGVNFPKFTDGRGYSTARLLRDR
                     YAYRGEIRAIGDVLQDQLFYMKRCGFDAFALREDKDIEAALSGLNVFSETYQAAVDQP
                     QPLFRRRAA"
     gene            2336..3058
                     /locus_tag="PLD53_07180"
     CDS             2336..3058
                     /locus_tag="PLD53_07180"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRC60732.1"
                     /translation="MAININLNDQALIDSVTAKTTAARNLLRQIATSYSPAVFANSLG
                     AEDMVLTDLIVGDTAKIEIFSLDTGRLPLETYALIDEVKKHYGLKLRLYYPRHDLVET
                     YTRENGINAFYESVELRKACCHVRKVEPLQRALAGRKAWITGMRAQQSATREGLPIQA
                     FDEGNGLEKFNPLSDWSEKEVWAYIKLNKVPYNALHDNFYPSIGCAPCTRAITPGEDV
                     RSGRWWWESPESKECGLHVKKA"
     gene            3173..4087
                     /gene="cysD"
                     /locus_tag="PLD53_07185"
     CDS             3173..4087
                     /gene="cysD"
                     /locus_tag="PLD53_07185"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRC60733.1"
                     /translation="MTTLTKQTLSHLDWLESEGIHIMREVAGQCSNPVLLFSGGKDSV
                     CMLRIAEKAFRPGKFPFPLMHIDTGHNYKEVIDFRDKRAAELGERLIVRSVEDSMARG
                     TVVLKSADESRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIFSFRDEFGQW
                     DPKNQRPELWDLYNARSFKGENIRVFPISNWTEFDVWQYIERENLELPSIYFAHQRQI
                     VRRNGGMLPVTDITPARPGDTIENVLVRFRTVGDISCTAPVESDADTVAKIIAETATT
                     TITERGATRLDDQTSDASMEQRKKEGYF"
     gene            4087..5376
                     /locus_tag="PLD53_07190"
     CDS             4087..5376
                     /locus_tag="PLD53_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRC60734.1"
                     /translation="MSAIENIQAVEIKDNGLLRFLTCGSVDDGKSTLIGRLLFDTKTI
                     LADTLHAIERTSKKRGMEAIDLSLLTDGLQAEREQGITIDVAYRYFTTGTRKYIIADA
                     PGHEQYTRNMVTAASTADLAIILIDARKGVLTQTRRHSYLTHLVNIPHVVVAINKMDL
                     VDYSQDVYDRIKADYMAFAAGLGILDVRFIPMSALNGDMIVDRGERLDWYQGPTLIDL
                     LEGATTAHVEHEECFRFPVQFVCRPQDSANPELHDYRGFMGRVESGVITVGDAVTVLP
                     AGRETRVKDIQVMGNSLPSAFAEHSVTLLLDDEIDISRGDMIVKTGELPIVARQFDAM
                     LCWLSESPLDPRRKYLIRHTTRDSKAMLAGIEFRMDINTMAHQAAEKLAMNDIAKVSF
                     KLAQPIFADRYAENRGTGAFIVIDESSNNTVGAGMIV"
     gene            complement(5399..6163)
                     /locus_tag="PLD53_07195"
     CDS             complement(5399..6163)
                     /locus_tag="PLD53_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60735.1"
                     /translation="MSVHLYFSLIPEALIASMLPPEQFGQYYATGHKFKSKGQAVFFE
                     IDPSFRHEYFDIEGALARCKAKPDGSPKNSVYVSMYRVMEHLSLAAIGRLYLTTTNGA
                     TLELSRGSRLPEPEKELHMYQDLTPINSMVVSVLDPAAYYADVTVKPSHSFSFPGMCF
                     VELELGPLALDPENGQEGDLPYSFLQHLREALMELRPETKENKLVHRVHSLVFPFRMV
                     KNGFYLGIGGELVFYPMLTLDVLRKDHSNWWRSANL"
     gene            complement(6284..>6825)
                     /locus_tag="PLD53_07200"
     CDS             complement(6284..>6825)
                     /locus_tag="PLD53_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRC60736.1"
                     /translation="THIRLDEVVQASQKDSKKVVEICTIYAQKGMFNIFMAVFFGTLA
                     CAFIEPFFFIGYLISLAIIGLYQAVFMANAGGAWDNAKKIVETEMHAKGTDLHNASVV
                     GDTVGDPFKDTASVAMNPIIKFTTLFGLLAVEMAVGLFAQGQQSVVYALAAVFLAINL
                     FFVFRSFYGMRIIAKKEAL"
BASE COUNT         1577 a         2003 c         1865 g         1380 t
ORIGIN      
        1 acgtatcctc gccagcatgc tggtgttgat tggcggaaaa ctgattacct tgtaaaaacg
       61 gtttgattta agaaggagcg acgcatgtat cgctacgaca gcattgacca acagatcatc
      121 aacgaacgcg ttgcgcagta tcgcgaccag atggaccgcc accaggccgg tgtactctcc
      181 gaagatgaac tgcggccatt gcgcctgcaa aatggccttt acatccagcg ccacggcccg
      241 atgctgcgaa tcgccattcc ctacggcatg ctttctgccg ctcaattgcg caaactggcc
      301 gaaatctcgc gccgttatga ccgcagcgtc gggcacttca ccacgcgcca gaacatgcag
      361 ctcaactggc cgaagtttga agagacgccc gagatacttg gcgaactggc gagcgtcgag
      421 atgcatgccg tacagacttc cggcaactgc atccgcaaca tcacgaccga tcaatttgcc
      481 ggaattgcac cggacgaact gaccgacccg cgcccgtatt gcgaagttct ccgtcagtgg
      541 tcgacattcc acccggagtt tgcttttctg ccacgcaagt tcaagatcgc cgtcaatgcc
      601 gcccgagaag atcgcgccgt gattcgcgcc cacgatgtgg ggctggaaat cgtcaacaac
      661 gacgccggtg agatcggtgt ccgggtgttt gtcggcggcg ggctcgggcg gactccggta
      721 ataggcaaag tcattcgcga gtttcttcca aggaagcatc tgctctcgta tctcgacgcc
      781 gtgctgcgcg tgtataaccg ctacggtcgg cgcgacaaca tgtacaaggc gcgcatcaag
      841 atactcgtca atgcgcttgg agttgaagaa ttcgcacgcc aggtggaagc cgattggtcg
      901 cgcatgaaag acggggaaac gacgctgacc gacgccgagt tcgatcgaat cgcgcaacat
      961 ttctctcccc cttcctacga aaacctgccg gcggacgact tcgcccatgc gacacgcctc
     1021 aaagaggaca aggccttcgc cagttgggcc aagcgctgcg tgcatgcgca caaggtgccg
     1081 ggttatgccg cggtcacgct ttcgctcaag gcaccggggg ctgcccccgg cgacatcacc
     1141 gatttacaga tggaagcggt tgccgatttg gctgaccgtt tcagctttgg cgaactgcgt
     1201 gtcactcacg agcaaaacgt ggtgcttgcc gatgtcagga agtgcgatct ttacgaggtt
     1261 tggaaaatcg ctcgcgccaa tgcactggcc acgcccaaca tcggactgct taccgacatg
     1321 atctgctgcc ccggcggtga tctttgcgca ctggccaacg cccgctcggt gccgattgcc
     1381 gaggcggtac aaagcaagtt tgatgatctc gactatctct acgacatcgg cgacatctcg
     1441 ctcaacattt cgggttgcat gaattcctgc ggtcatcatc atgtggccca catcggcatc
     1501 ctcggcgtcg ataagaatgg cgaggagtgg tatcaagtca ccatcggcgg ccagcaaggc
     1561 aatgagacca caatcggcaa ggtgatcggc ccgtcctttt atgccgatga agtgccgctg
     1621 gtcatcgaag cgctgatcaa cgtctatacc gaacagcgca cgccaaacga acgctttatc
     1681 gacacgctgc agcggatcgg catcgaacca ttcaagctgc gcgcctatgc cggacgcgac
     1741 aagcggaaac cggcaaatgc tgctgccgcc catatcaagg ccgactcggc caacgagacg
     1801 ctcacgaaag tcgccaatgc ctaagatcat caagaatggc caggtcgtcg ccgaccaccg
     1861 gcagctactc aaacttgacg gtgatcagac accggcatcc ctgaccctgc ctgacggaga
     1921 catcatggta cccttggcgg tgtggctggc gcgccaggag gaaatcctct cccgagacaa
     1981 ttcgccgggg gtctggcttg acagccacga ggacgtagaa gccattgccg gcgaccttga
     2041 acactttgcc gtcattggcg tcaattttcc gaagtttacc gacggccgtg gctactcgac
     2101 cgcccgcctg ctacgcgatc gctatgccta ccgtggcgaa atccgcgcca tcggcgatgt
     2161 actgcaggat caactgttct acatgaaacg ttgcggcttt gacgccttcg ccttgcgcga
     2221 ggacaaggac attgaagcgg cgctgtccgg tctcaacgtt ttcagcgaaa cctaccaggc
     2281 cgctgtcgac cagccacaac ctctgttccg ccgccgtgcg gcctaaggga tagccatggc
     2341 catcaacatc aacctgaacg accaggcatt gatcgactct gtaaccgcca agacaacggc
     2401 agcccgcaac ctgctccgcc agatcgcgac aagctattcg cctgcggttt ttgccaacag
     2461 tctcggcgct gaagacatgg tgctgaccga cctgatcgtc ggcgacacgg cgaaaattga
     2521 aattttctcg ctcgatactg gccgtctgcc attggaaacc tatgcgctga tcgacgaagt
     2581 gaaaaagcat tacggtctga agctcaggct gtattatccg cgccacgatc tggtcgagac
     2641 ctacacgcgc gagaacggca tcaacgcgtt ctacgaatcg gtcgaactgc gcaaggcgtg
     2701 ctgccacgtg cgcaaggtcg aaccgctgca acgggcgctg gccggcagga aggcctggat
     2761 aaccggtatg cgggcacaac agtcggcaac gcgtgaaggc ttgccaatac aggcgtttga
     2821 tgaaggaaat ggcctggaga agttcaatcc cttgtccgac tggtcggaaa aggaagtatg
     2881 ggcctatatc aaactcaaca aggttccgta caatgcgctg cacgacaatt tctacccaag
     2941 catcggctgc gcgccctgta cccgcgccat cacccccggc gaggatgtcc gctcgggacg
     3001 ctggtggtgg gaatcgccgg aatccaagga atgcggtctg catgttaaaa aggcttgaac
     3061 caccaagcca ccaaggacac caagtcgcac caagaagagc ataagaattt cttggtgaaa
     3121 ctttgtgctg ttggtgtctt ggtggtgaga tttttaagta tttaaggttc ttatgacgac
     3181 tctcaccaaa caaaccctct ctcacctcga ctggcttgaa tccgaaggca tccacatcat
     3241 gcgcgaggtg gccggacagt gcagcaatcc tgtgcttctc ttttccggcg gcaaggactc
     3301 ggtctgcatg ctgcgcattg ccgaaaaagc tttccgcccg ggcaagtttc ctttcccgct
     3361 gatgcacatc gacaccggac acaactacaa ggaagtcatc gactttcgcg acaaacgtgc
     3421 ggccgaactc ggcgagcgcc tgatcgttcg ttcggttgaa gattcgatgg cgcgtggaac
     3481 ggttgttctg aaatcggcag acgaatcgcg caacaagcac cagtcggtca cactgctcga
     3541 agcaattgaa gaattcggtt tcgacgcgtg catcggtggc gcacgccgcg acgaggaaaa
     3601 ggctcgtgcc aaagagcgta ttttttcgtt tcgagacgag ttcggccagt gggatccgaa
     3661 gaaccagcgt cccgaactgt gggacctgta caacgcgcgc agcttcaagg gtgaaaacat
     3721 tcgcgtcttc ccgatctcga actggactga atttgacgtg tggcagtaca tcgagcgcga
     3781 gaacctcgaa ttgccttcga tatattttgc gcatcagcga cagatcgtgc gtcgtaacgg
     3841 cggcatgctg ccggtcaccg acattacacc ggcccggccc ggcgacacca tagaaaacgt
     3901 gctggtacgc ttccgcaccg tgggcgacat aagctgtacg gcgccggtcg aatccgatgc
     3961 tgacacggtt gccaagataa tcgccgaaac ggcgacgaca acgattaccg agcgcggcgc
     4021 aacgaggctg gacgaccaga catcggatgc gtccatggaa caacgtaaaa aagagggtta
     4081 cttctgatgt ccgccattga gaacatccag gccgtcgaaa ttaaagacaa cggcctcctg
     4141 cgcttcctga cctgcggcag tgtcgacgac ggcaagagca cgctgatcgg ccgccttctg
     4201 tttgacacca aaacgattct ggccgacacc ctgcacgcca tagagcgcac gtcgaaaaaa
     4261 cgaggcatgg aagccattga cctctcgctg ctgaccgacg gacttcaggc cgagcgcgaa
     4321 cagggcatca ccatcgacgt cgcctatcgt tacttcacta ccggcacgcg caagtacatc
     4381 attgccgacg cgccggggca cgaacagtac acccgcaata tggtcaccgc cgcctcgacc
     4441 gccgatcttg ccatcatcct gatcgacgcg cgcaaaggcg tgctgacaca gacacgacgt
     4501 cactcctacc tcacccatct ggtcaacatc ccgcatgttg tcgtggccat caacaagatg
     4561 gatctggtcg actattcgca ggacgtctat gaccggatca aagccgacta catggccttt
     4621 gcggccggtt tgggcatact ggacgttcgc ttcataccga tgtccgcact gaatggcgac
     4681 atgatcgttg atcgcggcga acgactggac tggtatcagg ggccgacact gattgacctg
     4741 ctggaaggag caaccacggc gcacgtggag catgaggaat gctttcgctt cccagtgcag
     4801 ttcgtctgcc gtccacagga ttcagccaat cccgaactgc acgactaccg tggattcatg
     4861 gggcgtgtcg agtcgggtgt cattaccgtt ggcgatgccg tgaccgtcct ccccgccgga
     4921 cgggaaaccc gcgtcaagga catccaggtc atgggcaatt ccttgccttc ggcctttgcc
     4981 gagcactccg tcacgctcct gcttgatgac gaaatagaca tttcgcgtgg cgacatgatc
     5041 gtgaaaaccg gcgaactgcc aatcgtcgcc agacagttcg acgcgatgct ctgctggctt
     5101 tccgaatccc cgctcgaccc gcgccgcaag tacctgatcc ggcacaccac gcgcgacagc
     5161 aaggccatgc ttgccggcat cgagttccgc atggatatca acaccatggc gcatcaggcg
     5221 gcagagaagc tggccatgaa cgatatcgcc aaggtcagct tcaaactggc acaaccgatc
     5281 ttcgccgatc gctacgccga aaaccgtggt accggtgcct tcatcgtcat cgacgaatca
     5341 agcaacaata cggttggcgc ggggatgatt gtctgagctg aagcgcacga caatcaggtc
     5401 agagattggc cgagcgccac cagttgctgt gatccttcct aaggacatct agtgtcagca
     5461 ttgggtagaa gacaagttcg ccaccgatgc caaggtagaa gccgttcttg accatgcgaa
     5521 atgggaacac cagcgaatga acacggtgta ccagcttgtt ttccttcgtc tccggcctca
     5581 gttccatcag ggcttcccgc aggtgttgca ggaatgagta cggcaggtct ccctcctggc
     5641 cgttctccgg atcaagagcg agcggtccca gttcaagctc gacaaaacac ataccgggaa
     5701 aactgaagga atgcgagggc ttgacggtaa catcggcgta gtaggctgcc ggatccaata
     5761 cactcaccac catgctgttg atgggggtaa ggtcctgata catatgcagt tccttttcag
     5821 gctccggtag cctgctgccg cggctcaact ccagcgttgc gccgttggtc gtggtcaggt
     5881 acaacctgcc gatcgcggcc agcgaaaggt gctccatcac acgatacatg gagacgtaaa
     5941 ccgagttctt gggtgaacca tccggtttag ccttgcaacg ggccaaggcc ccttcgatgt
     6001 cgaagtattc atgacggaac gacggatcga tctcgaagaa tacggcttgc cccttcgatt
     6061 tgaacttgtg ccccgtcgcg tagtactgcc cgaactgctc tggcggcagc atggaggcga
     6121 tcaatgcttc gggaatcagc gagaagtaaa gatgtaccga catattgtat ctcccttaaa
     6181 agtgtcgact aggctcatca attgccgccg ttgccgcggc catttgtatg cgggaatagt
     6241 acgccatctc cattatatcc agaactcagc gcaccaaata ccgctacagc gcttcttttt
     6301 tggcgattat tcgcatgccg taaaaactgc ggaatacaaa aaagaggttg atggcaagga
     6361 acactgccgc caacgcatat accaccgatt gctgtccttg cgcgaatagc ccgactgcca
     6421 tctcgacagc aagcaaaccg aacaacgtgg tgaacttgat gatcggattc atggcaaccg
     6481 aagccgtatc tttgaacggg tcaccgaccg tatcgccgac taccgaggca ttgtgcagat
     6541 ctgttccctt ggcatgcatt tcggtttcaa cgatcttctt ggcattgtcc caggccccac
     6601 cggcgttggc catgaagact gcttggtaca agccgatgat cgccagcgag atcaggtagc
     6661 cgatgaagaa gaagggttcg atgaaggcac aagccagcgt gccgaagaac acggccataa
     6721 agatattgaa catgcccttt tgggcgtaga tcgtgcatat ctcgacgact ttcttgctgt
     6781 ctttctgcga ggcttgtacg acttcatcaa gcctgatgtg cgtgc
//
LOCUS       DAORPM010000123         6744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_2960_length_6744_cov_12.733293, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000123 DAORPM010000000
VERSION     DAORPM010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2960_length_6744_cov_12.733293"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /gene="flgA"
                     /locus_tag="PLD53_07205"
     CDS             <1..385
                     /gene="flgA"
                     /locus_tag="PLD53_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRC60737.1"
                     /translation="VTGNYLISTRAMPAGYVVGSTDVVVRSGDLSRLPVNVVTDQTQA
                     IGKIVKNGFSAGQPVRGDLLVDPWAVQQGQNVRTVSNGPGFSVSSEGKALNNAIAGQV
                     VQVRTASGQTVSGIARPGGIVEVSH"
     gene            534..818
                     /gene="flgM"
                     /locus_tag="PLD53_07210"
     CDS             534..818
                     /gene="flgM"
                     /locus_tag="PLD53_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRC60738.1"
                     /translation="MKIESSTKPAVAPRVNEPHGHSSSKAASVANDDVKLSFVSAQLS
                     TTDDQPPFDATRVSEIKQAISDGRFAINAGAIADRLIASAKELVDNQRQA"
     gene            833..1309
                     /locus_tag="PLD53_07215"
     CDS             833..1309
                     /locus_tag="PLD53_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HRC60739.1"
                     /translation="MTAPTTAFQQTLDAEAKAVQQFIGLLQLEQTALSTGKTDELPAL
                     IDKKAGLAARLNALAAQRNQSLAEQGFAADLVGVESWCAKNPQEKNVLDAWQRILSLA
                     SEARELNRLNGELIQMRMQYNAKALEALQGGKDSFDLYGPDGQSKSPSKRRISDAV"
     gene            complement(1320..2108)
                     /gene="motD"
                     /locus_tag="PLD53_07220"
     CDS             complement(1320..2108)
                     /gene="motD"
                     /locus_tag="PLD53_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HRC60740.1"
                     /translation="MARRKHEEEHDNHERWLVSYADFITLLFAFFVVMYALSTVNEGK
                     YRILSDSMSSAFRNVQVNSSSPLPVTLTPPIPVIQKPRAAERAQEAVKQKRRDKMRNV
                     AQDILEVMAPLIEQGKVRVIETSRGVTIEINDSILFSPGQALLQPALVKAMGAIADVL
                     APTDFPITIEGHTDNVPINTLQFPSNWELSAVRATTVLRLFVDSGVAPDRLTAIGFAD
                     TRPVEPNLLADGRARNRRVTILIDSADPEKGKEVDLESRPVSQP"
     gene            complement(2112..2852)
                     /locus_tag="PLD53_07225"
     CDS             complement(2112..2852)
                     /locus_tag="PLD53_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HRC60741.1"
                     /translation="MDKISVLGLLFGIVAIIGGQLLEGGHIASLSQPTALLIVLGGTM
                     GAVMLQSPYATFMRGMRMVRWVWYPPVVDHQQLIKQISNWSQVSRREGLLALEGVINQ
                     LKDDFSRKGLQLLVDGAEPERLREVLEVDISTFEVEMKLSARIWEAAGGYSPTIGILG
                     AVLGLIHVMENLSDPSKLGAGIAVAFVATIYGVGLANLVFLPIANKLKAHIGRLIVQR
                     EMIVDGLVGIANGDNPRIIESRLKGYIF"
     gene            complement(2845..3588)
                     /locus_tag="PLD53_07230"
     CDS             complement(2845..3588)
                     /locus_tag="PLD53_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HRC60742.1"
                     /translation="MYNAAGQIDKDQLIQRFAPLVKRIAYHLMARLPSSVQVDDLIQN
                     GMMGLLDAINRFEAGMGAQFETYAAQRVRGAMLDGLRENDWLPRSLRRDFRRIEVAIS
                     QLEQEYGRTPSESELAETLGMALAEYQKMLQDARGHQLISFEDMIDDGNEDFLERHLS
                     DDSNEPSRILEDESLRKSLVQGIELLPEREKLMMALYYEQDLNLREIGEVMGVTESRV
                     CQLHSQAVARLRARIFGDTGLRKKAKKNG"
     gene            complement(3601..4485)
                     /locus_tag="PLD53_07235"
     CDS             complement(3601..4485)
                     /locus_tag="PLD53_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC60743.1"
                     /translation="MAEAQRDQAAGLRRLFGRKPPRIVTFAAGSVGVGKSILVANLAA
                     ALARQGKEVLVLDENTKKNVASCFGALARHDLLHVINRERALSEAILTVAPGVRVLPA
                     ARAVTKLGRLSLAQQESFLESITGMERQADVILVDASLDHPLGFSPLGLAAHDTVVVI
                     SPTGSSITDAYALIKKVSLGYARKNFRILVNKVRSLDEAKSIYGNISKLARSRGLARL
                     EYTGYVLLDECLRQASHLSQPVDGLFPDSPAARAYRTIASDLLTWPSSGEEPGGLEEF
                     VQQLLHLSKQIDPIAIYA"
     gene            complement(4478..5815)
                     /gene="flhF"
                     /locus_tag="PLD53_07240"
     CDS             complement(4478..5815)
                     /gene="flhF"
                     /locus_tag="PLD53_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRC60744.1"
                     /translation="MNVRKFIAASARDALHKVKELLGPDAIILSNRAIPGGVEIMAVA
                     ARDMEMIVPTPTREKQSPNLNPKPNDDYTVRLSTPTTNLQPKAPPKVTVAAPQQQPQR
                     FPPLAPRAAPPPVAETAAAVKPQAEIVPAEVMDEIRSLRKMFEQHLAGVAWGETARSE
                     PVKTEILRQMLDAGFSPQLARELLAALPSELNAAKALAWVKVGADRSLLTIAPENDIV
                     DRGGIYALVGPTGVGKTTTTAKLAARCVLRHGASKVALVTTDGYRIGAHEQLRIYGRI
                     LGVSVHLVKDAEELSQTLSDLQHKHMILVDTMGMSQRDRMVGEQVAMFENSNVKRLLL
                     LSATGRGDTLDDVVRAYSGPDLAGCILSKVDEAAGIASAIDVIIRHGLRLHYVSNGQR
                     VPEDLHLPNRAYLLHRAFKDVPEASPHRLGGFEPGLMMASAGSGMVAAGGRRG"
     gene            complement(5812..>6744)
                     /locus_tag="PLD53_07245"
     CDS             complement(5812..>6744)
                     /locus_tag="PLD53_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HRC60745.1"
                     /translation="IRGIRKKFAQEVGFLVAPVHIRDNLELKPNAYRILLKGVEIGQG
                     EAFPGNLLAINPGRVAGQLPGAETKDPAFGLPAVWIDTALRDQAQAYGYTVVDASTVA
                     ATHLNHLILSHAAELLGRQETQALVDHLAKEMPKLVEDLVPKVLPLGVLQKVLQNLLE
                     EDIPIRDMRSIIETLAENATRSQEAEVLTAQVRVALGRSITQQLYPSGAEMQVMSLDP
                     QLERILQQAIAGGGDNASIEPSLADSLIRETSTAAQRQEELGLPAVLLVSGNLRLLLS
                     RFLRRGIPQLKVLAHNEVPESKIIKVTSIIGGRV"
BASE COUNT         1502 a         1966 c         1824 g         1452 t
ORIGIN      
        1 cgttaccggc aactacttga tctcaacgcg ggcgatgcct gccggatacg tagtcggttc
       61 aacagacgtt gttgtccgca gtggcgatct gagcaggttg cccgtaaacg tcgtcactga
      121 tcagacacag gctatcggca agatagtgaa gaatggattc tcggccggtc agccagttcg
      181 tggggacctg ttggtggacc catgggcggt ccagcagggg caaaatgtca gaaccgtctc
      241 gaacggcccc ggattcagcg tcagcagtga aggcaaggca ctcaacaatg cgatcgcggg
      301 tcaagtggtt caggtccgca ccgcctctgg tcagacggtt agcggcatcg cccggccagg
      361 tgggatagtt gaagtatctc attgagccaa agagttggtt taacggcagc ttttcactcc
      421 attaccgccg gcattgctcc agacaaacct aatgacaccg tgaattggtg ctaaagtttc
      481 atcggttgga gccgttagtt cgcctaagta gaatactttt ttgaggcatc atcatgaaga
      541 tcgagagcag taccaagccc gctgttgcgc cacgtgtcaa tgaaccgcat gggcattcca
      601 gcagcaaagc agcgtcggta gcgaacgatg acgtcaagtt aagttttgtt tccgcgcagt
      661 taagcaccac ggatgatcag ccgcccttcg acgcgacccg tgtgtcggaa atcaagcagg
      721 ccatctcgga tggtcgtttc gcgatcaatg ctggggccat cgccgaccgc ttgattgcct
      781 ccgccaagga acttgtggac aaccagcggc aggcctgact ttggctatta ctgtgacggc
      841 cccaacgacc gcctttcagc agactttgga tgccgaggct aaggccgttc agcagtttat
      901 tggtctgctt cagcttgagc agactgcgct aagcaccggg aaaaccgatg aattgcccgc
      961 cctgatagac aaaaaagcgg gccttgccgc tcgtttgaac gctctcgctg cacagcgcaa
     1021 ccaatcgctt gccgagcagg gatttgccgc cgacctcgtt ggcgtggaat cctggtgcgc
     1081 aaaaaatccg caagaaaaaa acgttcttga cgcatggcaa cggattctgt ctctggccag
     1141 cgaagcccgg gagttgaatc ggctcaatgg tgaattgata cagatgcgta tgcaatacaa
     1201 tgccaaggct ttggaagccc tgcagggcgg caaagattcc tttgatctct acggcccgga
     1261 cgggcaatcg aaatcgccga gcaaaaggcg catttccgac gctgtgtgat ccccgtaatt
     1321 tatggctgcg ataccggccg cgattcgaga tcgacctctt tgcctttctc tggatcagcc
     1381 gagtctataa gaatcgtcac ccggcggttg cgcgcccgac cgtcggccag aaggtttggc
     1441 tctacggggc gcgtatcggc gaaaccaatg gcggtcagtc tatccggcgc cacgccggaa
     1501 tcaacgaaca ggcgcaggac tgtcgttgca cgaacagccg aaagctccca attggaggga
     1561 aactgtaggg tattgatcgg cacgttgtca gtatgtcctt cgatggtaat cgggaaatcg
     1621 gtcggcgcca gtacgtccgc aatcgcgccc atggccttga ccaaagcagg ctgcaaaagc
     1681 gcctgcccag gtgagaatag aatgctgtcg ttgatctcga tcgtcacgcc acggcttgtt
     1741 tcgatcactc gcaccttgcc ctgctcgata agtggtgcca tgacttccag aatgtcctgg
     1801 gcgacatttc gcatcttatc gcgtcgcttt tgtttcaccg cctcctgcgc cctctccgcc
     1861 gctctcggtt tttgaattac cggtatcggc ggcgtcaatg ttactggcag cggcgacgaa
     1921 ctattgactt gcacgttgcg aaaggcgctc gacattgaat cgctcagaat acgatacttc
     1981 ccctcattga ccgttgaaag cgcatacatc accacaaaga acgcaaacag cagagtaatg
     2041 aaatccgcat acgaaaccag ccagcgctcg tggttatcgt gctcttcttc gtgtttgcgg
     2101 cgtgccataa gctaaaaaat gtaacccttg agacggcttt cgatgatccg aggattgtcc
     2161 ccgttggcaa taccaactag gccatcgaca atcatctcgc gctggactat caggcgaccg
     2221 atatgcgcct tgagtttgtt ggctatcggc agaaatacca gattggcgag ccccacgcca
     2281 tagatggtag cgacaaacgc aaccgcgatc ccggcaccca gtttggatgg atcggaaaga
     2341 ttttccatga cgtggatcag acccagcaca gcgcccagaa tgccgatggt cggcgagtag
     2401 cctcccgcgg cttcccatat tcttgccgac agtttcattt ccacttcaaa cgtgctgatg
     2461 tcgacctcaa gtacctcgcg caaacgctcc ggctcggccc catcgaccag caattgcagg
     2521 cccttgcggg aaaaatcatc tttcagttga ttgatcacgc cttccagcgc caacaagccc
     2581 tcacggcggg aaacctggct ccagttggat atctgcttga tcaattgctg gtggtccacg
     2641 accggcggat accacaccca tcgaaccatc cgcattccgc gcataaaggt tgcgtaggga
     2701 ctctgcagca tgactgcacc catcgtaccg ccaagcacga tcagcagcgc cgtcggctgc
     2761 gagagcgatg cgatatggcc gccctcgagt agctgaccgc caatgatcgc cacaataccg
     2821 aacagcaatc cgagaacgct gatcttatcc attcttcttg gccttcttcc tgaggcccgt
     2881 atcgccaaaa atccgtgcac gcaacctggc tacagcctga ctatgcaact ggcagacacg
     2941 tgattctgtg acacccatga cctccccgat ctcgcgcagg ttgagatcct gttcgtaata
     3001 gagtgccatc atcaattttt cacgctcggg cagcaactcg ataccctgca ccagtgattt
     3061 gcgcagactc tcgtcctcca gaatccgtga cggttcgttc gagtcgtcag acaaatgccg
     3121 ttcgagaaaa tcctcattgc catcatcgat catatcctcg aaggaaataa gttgatgccc
     3181 tcgtgcatcc tgtagcatct tttggtattc ggcaagtgcc atgccgagcg tttcggctag
     3241 ctccgactcg cttggcgtac gcccgtactc ctgttcgagt tgggaaatgg cgacttcaat
     3301 gcgacgaaaa tcccgccgca aactgcgcgg caaccagtca ttctcgcgca aaccatccag
     3361 catggcaccg cgcacccgct gggcagcata ggtttcaaac tgcgccccca ttcctgcctc
     3421 aaagcgattg atggcatcca gcaaacccat catgccattc tggatcagat cgtcgacctg
     3481 aacacttgaa ggcaatcggg ccatcagatg ataggcgatg cgcttaacga gcggagcgaa
     3541 gcgctgtatc aactgatcct tgtcgatctg cccagcagcg ttatacatgt gttggtggcg
     3601 tcaagcgtaa atcgctatcg gatcgatctg cttgctcagg tgtaatagct gttgcacgaa
     3661 ttcttcgagt ccgccgggct cctcgccgga cgacggccag gtgagcagat cactggcaat
     3721 cgttcgatac gcccttgcgg caggggagtc gggaaagagg ccatcgacag gctgggacag
     3781 gtgcgatgcc tggcgcaagc actcgtccag aagcacgtaa ccggtgtatt caaggcgcgc
     3841 caggccgcga ctgcgagcaa gcttggaaat attgccataa atcgacttgg cttcatccag
     3901 actgcgcacc ttattcacca ggatccggaa gttcttgcgc gcgtagccca aactgacttt
     3961 cttgatcagt gcataagcat cggtaattga cgaaccggta ggcgaaataa ccaccaccgt
     4021 atcgtgcgcg gctaggccaa gcggggaaaa gccaagcgga tggtcaaggg aggcgtccac
     4081 cagaatcaca tccgcctgac gctccatgcc agtgatgctc tccaaaaaag attcttgttg
     4141 cgcaaggctc aatctcccga gtttcgtcac cgcacgcgct gccggcaaga ctcgaacgcc
     4201 aggcgccacc gttaaaatcg cctcggaaag cgcccgctct cgattgatta catggaggag
     4261 gtcgtggcgc gcgagcgctc cgaaacagga cgcgacattc ttctttgtat tctcgtccag
     4321 aaccaggact tccttgcctt ggcgagccag ggcagcggcg aggttggcta ccagtatgct
     4381 ttttccaacg ccgacactac cagccgcaaa agtgacgatg cgcggcggct ttcggccaaa
     4441 aagccgacgc agaccagcag cctggtccct ttgagcctca gccacgacgg cctccggcgg
     4501 caaccatgcc ggaaccggca ctggccatca tcaggcccgg ctcgaagccg cccaagcggt
     4561 gcggcgaagc ttccggcaca tccttgaaag cacggtgcaa tagataggca cgattgggca
     4621 gatgcagatc ttctggcacg cgctgcccat tcgagacgta gtgcagccgc aaaccgtgac
     4681 ggataatgac atcgatcgcc gatgcgatcc ctgccgcctc gtcgaccttg ctcaaaatgc
     4741 atccggcaag gtctgggccg ctataagcgc gcactacgtc gtcgagggtg tctccacgcc
     4801 ccgtggcgga aagcagcagc agacgtttga cattgctgtt ttcaaacatc gccacctgtt
     4861 caccaaccat tctgtcacgc tgactcatgc ccatcgtatc gacaagtatc atgtgcttgt
     4921 gttgaagatc ggacagcgtc tggctcaact cttcagcgtc ttttaccaga tgcaccgaca
     4981 caccgagaat gcgaccataa atccgcagtt gttcgtgagc cccgattcgg taaccatccg
     5041 tcgtgaccag cgccactttg ctcgctccgt gacgcagaac gcagcgggcc gcaagcttgg
     5101 cggtagtcgt cgttttgccg acaccggtcg ggccaaccag tgcgtagatt ccgccacggt
     5161 cgacgatatc gttctcgggt gcgatggtca gcaaggaacg atcagcaccg accttaaccc
     5221 aagccagtgc cttcgccgca ttgagttcgc tgggcaatgc agcaagcagt tcgcgagcca
     5281 attgtggaga gaagcccgcg tcaagcattt gacgcagaat ttccgtttta acgggctccg
     5341 agcgcgcggt ttctccccag gcaacaccag ccagatgttg ctcgaacatc tttcgtaacg
     5401 agcgaatttc gtccatcact tccgccggaa ctatttcagc ctgcggcttg acggcagctg
     5461 ccgtctcggc aactggcggc ggtgcagcac ggggcgccaa tggcggaaag cgctgcggct
     5521 gttgctgagg cgcggctaca gttaccttgg gaggtgcttt gggttgcaag ttggtcgtcg
     5581 gagtcgaaag acgtaccgta tagtcatcat ttggcttcgg attcagattg ggactctgct
     5641 tctccctggt gggggtcggc acaatcattt ccatatcgcg ggcggcaacc gccatgattt
     5701 cgacgccccc cggaatcgcc cggttggaca ggatgatcgc atcggggccc agtaactcct
     5761 tgaccttgtg caatgcgtcc cgagcggaag ccgcgatgaa ttttctgacg ttcataccct
     5821 gcctccaatg atcgaggtga ccttgattat tttgctttca ggtacttcgt tgtgagctag
     5881 caccttcaat tgaggaatgc ctcgcctaag gaagcgggac agaagtaggc gaagattccc
     5941 tgaaaccagc aatacggccg gcagcccaag ttcttcctga cgttgcgccg ctgtcgatgt
     6001 ctcgcgaatc agtgagtcag caagactcgg ctcgatactg gcattgtcgc caccacccgc
     6061 gatggcctgc tgaagaatgc gctcaagctg cggatcaagc gacatgacct gcatttctgc
     6121 tccactcgga tagagttgct gagtgatcga gcgaccaagt gcaacgcgca cttgtgcagt
     6181 cagcacttcg gcctcctgcg agcgcgtcgc gttttctgca agcgtctcaa tgatggaacg
     6241 catgtcgcgt atggggatgt cttcttcgag aaggttctgc aagacctttt gtagaacgcc
     6301 aagtggcagc actttgggca ccagatcttc aacgagtttg ggcatttcct tcgccagatg
     6361 atcgacaagc gcttgcgttt cctgacgtcc aagcagttct gccgcatgcg acagaatcag
     6421 gtgatttaga tgcgtagcgg caacagtgct ggcatcgacc acggtatagc catacgcttg
     6481 agcctgatcg cgcagcgcag tgtcaatcca gacagcaggc aaaccaaagg ccggatcctt
     6541 ggtttcggca cccggcaact ggccggcaac acgccccggg ttgatcgcca acaggttgcc
     6601 cggaaatgct tcgccctggc cgatctcgac accttttagc agaatccggt aagcattggg
     6661 cttcaactcc agattgtcgc gaatgtgcac cggggcaacg agaaaaccta cttcctgtgc
     6721 aaacttcttg cgtattccgc gaat
//
LOCUS       DAORPM010000124         6681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3008_length_6681_cov_13.749774, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000124 DAORPM010000000
VERSION     DAORPM010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3008_length_6681_cov_13.749774"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1208
                     /locus_tag="PLD53_07250"
     CDS             153..1208
                     /locus_tag="PLD53_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HRC60746.1"
                     /translation="MQAARWSDLPGWLEDDLAASLPGFLSSCRALASKPQWLLWRAAC
                     EEAKGLAPNDSAAVRRFYESRFEPYLLTNPDNTTNGLVTGYYEPLLHGSRRRSANFAQ
                     PVLGVPSDLLTIELADVLPDLKNMRLRGRLQGNKVIPYYPRADIISREQDYADRVLLW
                     VDDPVELFFLQIQGSGRVKLPDGKLVRIGYADQNGHPYRSVGRVLIDRGELQPDQASM
                     QGIQAWARANPSKLDEVLNANPSYVFFREVPSNGNENAGPQGALGVPLTPGRSIAVDP
                     RHVPLGAPVFLSTTQPNSSAPLRRLTLAQDTGGAIRGVVRADFFWGFGPDAGSQAGRM
                     KQQGQMWVLLPPGAGPK"
     gene            1369..2571
                     /locus_tag="PLD53_07255"
     CDS             1369..2571
                     /locus_tag="PLD53_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRC60747.1"
                     /translation="MEALKSGSDVLFILLGGIMVLAMHSGFAFLELGTVRKKNQVNAL
                     VKILVDFSISTIAYFFIGYSIAYGVNFFAGAETLMQKNGFELTRFFFLLTFAAAIPAI
                     ISGGIAERARFNPQLVATFLIVAFIYPFFEGIAWNQAYGVQAWLKASFGEEFHDFAGS
                     VVVHAMGGWIALPAVVLLGARYGRYTKEGRVSAHPPSSIPFLALGAWILTVGWFGFNV
                     MSAQTLDKISGLVAMNSLMAMVGGTLVAVVLGKNDPGFVHNGPLAGLVAVCAGSDIMH
                     PMGALVVGGVAGALFVVMFTLTQNRWKIDDVLGVWPLHGLCGAWGGIAAGIFGQKALG
                     GLGGVAFVPQLLMTGMAISVALAGSTIVYSVLRSTIGLRLDREEEFDGADLSIHRISS
                     TPEHESNW"
     gene            2694..3155
                     /locus_tag="PLD53_07260"
     CDS             2694..3155
                     /locus_tag="PLD53_07260"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRC60748.1"
                     /translation="MSALELKVPPVAVVLVFGVAMWLVSVNVPSLAIGSPWRIVVATA
                     LAGAGLVISVIAVVAFRKARTTVNPTRPGTTSALVTSGVYGLSRNPMYVGILLALTGW
                     AVFLSHTLAFAFLPAYVAYMNRFQISPEERALSVNFGSEFVAYKQSVRRWL"
     gene            3264..4253
                     /locus_tag="PLD53_07265"
     CDS             3264..4253
                     /locus_tag="PLD53_07265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011254937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRC60749.1"
                     /translation="MKTRQLGRNGPQVSALGLGCMGMSDFYTGRDDAESIATIHRALE
                     LGINFLDTADIYGPHTNEELVGRAIKGRRSDVFLATKFGILRDPKDPLVRGFDSRPEY
                     VRRAVESSLQRLGVTTIDLYYQHRVDPNVPIEDTVGVLADLVRQGKVRYIGLSEASAA
                     TLARAHNIHPITALQTEYSLWTRDPELEVLAACRRLGISFVPYSPLGRGFLTGAITRP
                     EDFAADDYRRSSPRFQGGNFSKNLELVEKVKSFAVEVGCTPAQLALAWVLAQGDDIVP
                     IPGTRRRKNLEENMGALDVQLTSAQLAELSEVFPFKAASGDRYPANMMGAVNR"
     gene            complement(4396..6384)
                     /locus_tag="PLD53_07270"
     CDS             complement(4396..6384)
                     /locus_tag="PLD53_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HRC60750.1"
                     /translation="MFKKILIANRGEIACRVIKTARRMGIATVAVHSEADKDSLHVLS
                     ADEAVCIGPAASKDSYLKIDNIIAACKQTGAEAVHPGYGFLSENQEFSKRLEENGITF
                     IGPKHHSVAAMGDKIESKKLALKAKVNTIPGYNDAIADAKQAVEIAKGIGYPVMIKAS
                     AGGGGKGLRVAFNDTEASEGFDSCRNEARNSFGDDRVFIEKFVEGPHHIEIQIIADAH
                     GNTVYLLERECSIQRRHQKVIEEAPSPFIDDAIRKAMGEQAVALAKAVDYQSAGTVEF
                     VVGSDKSFYFLEMNTRLQVEHPVTEMITGLDIVELMIRIAAGEKLPFAQADIKPKGWA
                     IECRINAEDPFRGFMPSTGRLIRFQPPEAVENEVRVDTGVYEGGEISMHYDSMIAKLI
                     CHGDDRNQAIERLREALNVFLIRGLSSNIPFQAALVQHPRFVSGIFTTAFLAEEYPEG
                     FVAADVPYDDPGLLAAVAAFARRRYIERAVKISDQMPGHERKVGRDWVVAIEGTHHKV
                     TLDPIPGGYSAHCGDKHYDIVSDWLFRELVFRGTCNGKPFCMQVERDGLAYRISHYGK
                     QVKAVVMTPKADLLLARMPVKLPPDLSKFLLSPMPGLLREILVQDKQQVRAGEKLAVI
                     EAMKMENILKAEKDCLVKRIVAEPGEILSVDQVIIEFA"
     gene            complement(6535..>6681)
                     /locus_tag="PLD53_07275"
     CDS             complement(6535..>6681)
                     /locus_tag="PLD53_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRC60751.1"
                     /translation="NPFVAGARGFIDDVIMPNETRKRICRSLAMLHDKQLTNPWRKHG
                     NIPL"
BASE COUNT         1257 a         1916 c         1978 g         1530 t
ORIGIN      
        1 ttcccctgct cgctgcaatc cttcttgcga ccttgctcgc cgcctgtgcc acggtaaagg
       61 aggcgcccgt cgcgtcctgc ccggtgtgtc ccgtgtgcca gcaatgtccg gcacaaccag
      121 ttacccccgt ggttttgcca ccggccaagc cgatgcaggc cgcgcgctgg agcgatctgc
      181 cgggctggtt ggaggacgac ctggccgcct cgctaccggg ctttttgagt tcgtgccgtg
      241 ctttggcgag caagccgcaa tggttgttgt ggcgggccgc ctgcgaagaa gcgaaagggc
      301 tggcaccaaa tgacagcgct gcggtgcgcc gcttttacga gtcccgtttc gaaccctacc
      361 tgctgaccaa tcccgacaat acgaccaacg ggttggttac cggctattac gaacctctcc
      421 tgcacggctc gcgcagacgc agtgcgaatt ttgcgcagcc ggtattgggc gtaccgtccg
      481 acctgttgac catcgaactg gccgacgtgc tgccggatct gaagaacatg cgtctgcgcg
      541 gccggctgca gggaaacaag gtcataccgt actatccccg cgcagatatc atttcccgcg
      601 aacaggacta cgctgatcgc gtgctgctct gggtggatga tcccgttgaa ctctttttcc
      661 tgcagattca gggctccggt cgtgtcaaac tgcccgatgg aaagctggtg cgaatcggtt
      721 acgccgacca gaacggtcac ccctaccgtt cggtcgggcg cgtactgatc gaccgcggtg
      781 agctccagcc tgaccaggcc tccatgcagg gtattcaggc ctgggcgcga gccaacccgt
      841 caaagctgga cgaagtgctc aatgcaaatc caagctacgt gttcttccgc gaagtgccgt
      901 ccaacggaaa tgaaaacgcc ggaccgcaag gtgccctggg cgtgccactg acccccggac
      961 gcagcattgc cgttgatccg cgacatgtgc cgctcggagc gccggttttc ctgtccacga
     1021 cacagcccaa ttcgtccgca cccttgcgcc gtttgacgct ggcgcaggat accggcggtg
     1081 cgattcgcgg ggtcgtgcgt gccgactttt tctggggttt tggccccgat gcaggaagtc
     1141 aggccggacg aatgaagcag caaggtcaga tgtgggttct tctgccgcca ggcgccgggc
     1201 caaaataggg cacggaagag gttccatttc ggtgcacatt gcagtgcgag cgcaccaacg
     1261 cggtgccaag ttccttgttg gcttgttcct aactcattga aatataagaa cacgcctcga
     1321 tggaatagaa cttgcaagcg tgccccaaat tgaaatccag gaggtcgcat ggaagcactc
     1381 aagtcgggta gtgacgtact gttcatcttg ttgggcggca tcatggtgct ggccatgcat
     1441 tctggctttg cctttctcga actgggcacg gttcggaaga agaatcaggt gaacgcgctg
     1501 gtcaagattc tcgtcgactt ttcaatatcg acgatcgcct atttcttcat cggttattcg
     1561 attgcctacg gcgtcaactt ctttgctggc gctgaaacgc tgatgcagaa aaacggtttc
     1621 gagctgaccc gtttcttttt cttgctcacc tttgccgcag caattcccgc aatcatttcc
     1681 ggaggcatcg ccgagcgcgc cagattcaat ccgcagctgg tcgccacgtt tctgatcgtc
     1741 gccttcatct atcccttctt cgagggcatc gcgtggaatc aggcctatgg ggttcaggct
     1801 tggctgaagg ccagtttcgg ggaagagttc cacgacttcg ccggctcggt tgtcgtacat
     1861 gccatgggcg gctggattgc gctgcctgcg gtggtattgc tcggcgcacg ttatggacgc
     1921 tacaccaagg aaggacgggt ttcagcgcat ccgccgtcat caattccttt tcttgccctc
     1981 ggcgcgtgga tcctcaccgt tggctggttc ggcttcaacg tcatgagcgc tcaaacgctc
     2041 gacaagattt ctggcctggt cgcaatgaac tcgttgatgg ccatggtcgg tggcacgctg
     2101 gtggcggtcg tgcttggcaa gaacgacccc ggatttgttc ataacggccc gctggcaggg
     2161 ctggtggccg tgtgtgcagg ttcggacatc atgcacccga tgggcgcgct ggtggtgggt
     2221 ggcgtcgccg gcgcattgtt cgtcgtcatg ttcacactga cccagaaccg ctggaagatt
     2281 gacgacgtgc tcggtgtctg gccgttgcac ggcctgtgcg gcgcctgggg cggcattgcc
     2341 gccggcatct ttgggcaaaa ggctttgggc ggactcgggg gtgtcgcgtt cgtaccgcag
     2401 ttgctgatga ccggcatggc gatttccgtt gcgctggccg gcagtaccat cgtatacagt
     2461 gtgctcaggt caacaattgg ccttcgtctt gatcgggagg aagaattcga tggcgcagat
     2521 ctcagcatcc accgtatctc atccacgccg gagcatgagt cgaactggtg attctgtcac
     2581 ggtagggatt tgtccggtcc agccgccgga tgcatgcccg tacgtccggc ggtgtgggag
     2641 ggaggcgccg taaacgcccc tttctattcc gattgtgcat cggcctcgac cgcgtgagcg
     2701 ctctcgaact caaagtcccg ccggttgctg tcgtgctggt ttttggcgtc gccatgtggc
     2761 tcgtctctgt taacgtgccg tcgctggcaa ttggctcgcc gtggcgtatc gtggtcgcca
     2821 ccgcccttgc cggtgcaggc ctcgtcatca gcgtgatcgc ggtggttgcg ttccgcaaag
     2881 ccaggacgac ggtcaacccc accagacccg gcaccacgtc ggccctggtt acctcgggtg
     2941 tttacggcct gagccgaaat cccatgtacg tcggtatcct cctcgcgctc accgggtggg
     3001 cagtctttct ctcccataca ctggccttcg catttctgcc cgcctatgtt gcgtacatga
     3061 atcgcttcca gatctcaccc gaggagcggg cgttgtccgt taacttcggc tcagagtttg
     3121 tggcttacaa gcagtccgtt cgcaggtggc tatgaggcac gacaggtttt ttgcaggcga
     3181 aaccgccaat gattgcctga gcaacggtgt ctgggttgat gcagtgtgtt aaccttagat
     3241 cttgcttttc cgtaaggggg tcaatgaaga cacgacagtt gggccgtaat ggtccgcaag
     3301 tatcggctct tggcctcggc tgcatgggga tgagcgattt ttatacgggg cgagacgacg
     3361 ccgaatcgat tgccacgatc catcgcgcgc ttgagcttgg catcaacttt ctcgacaccg
     3421 ccgacatcta cggtccgcac accaacgaag aacttgtagg gcgcgccatc aaaggtcgga
     3481 gaagcgatgt cttcctcgcg actaagtttg gcatcctgcg cgacccgaag gacccgctgg
     3541 tgcgcggctt cgacagccgg cccgagtatg tgcgccgtgc ggtggaaagc agcctgcagc
     3601 gtctcggcgt gacgacgatc gacctctatt accagcaccg ggtcgatccg aatgtgccga
     3661 tcgaggatac ggtgggtgtg ctcgccgatc tggttcgaca gggaaaggtg cgctacatcg
     3721 gtctgtccga ggcttccgcg gccacgcttg cgcgcgcaca caacatacat cccattaccg
     3781 cgttgcagac cgagtattcg ttatggacac gcgacccgga acttgaggtg cttgccgcct
     3841 gtcgccgact ggggatcagc ttcgttccgt acagtccgct cggacgcgga tttctcactg
     3901 gcgcgattac ccggcccgag gatttcgccg ctgacgacta ccgacgttcg agtccacgtt
     3961 tccagggagg caatttttcg aagaaccttg agctggtcga aaaggtcaag tcctttgccg
     4021 tcgaagtggg ctgcactccc gcgcaacttg cattggcctg ggtcctcgct cagggtgacg
     4081 acatcgttcc gatcccgggc acccggcggc ggaagaatct cgaagaaaac atgggtgcgc
     4141 tggacgttca acttacatcc gcccaacttg ccgagctgag tgaggtgttt ccgttcaagg
     4201 cagcgagtgg tgaccgatat cccgcgaaca tgatgggcgc agtcaatcgc tgagctctga
     4261 gcactgtcgt ccgagcgctt tttgaacagg ctcaaataaa gaaaagccgg ggagtgcaga
     4321 acgtctcccc ggctttgcct tgttgtttcg aaggcagagg cgtcagatac gcctctagcc
     4381 ctcgaatgga ttgacttacg cgaactcgat aatgacttga tcgactgaca ggatttctcc
     4441 gggctcggca acgatcctct ttaccagaca gtccttctcc gccttgagga tgttttccat
     4501 cttcatcgct tcgatgacag ccagcttttc tccggcgcga acctgttgtt tgtcctgaac
     4561 cagaatttcc cgcagcaggc cgggcattgg ggagagcagg aactttgaga ggtccggcgg
     4621 cagtttcacc ggcatccttg ccagcagaag gtctgccttt ggcgtcatta caacggcctt
     4681 gacctgcttg ccgtagtggc tgatgcgata cgccagaccg tcacgctcga cttgcatgca
     4741 gaacggcttg ccgttgcagg ttccacggaa taccagttcc ctgaaaagcc agtccgatac
     4801 gatgtcatag tgtttgtcgc cgcagtgggc cgaataaccg ccgggaatcg gatcaagagt
     4861 gaccttgtga tgtgttcctt cgatggctac cacccagtcc ctgcccactt tgcgctcatg
     4921 gccaggcatc tggtcgctga tcttgaccgc gcgttcgata taacggcgcc gagcaaacgc
     4981 ggcgacggcg gccagcaacc cggggtcgtc atagggaacg tcggcagcga cgaagccttc
     5041 cgggtattct tcggcgagaa aggccgttgt aaagatgccg gaaacaaaac ggggatgctg
     5101 caccagcgcg gcctggaagg ggatattcga gctgaggccg cgaatcaaga agacgttcaa
     5161 cgcttcacgc aggcgctcga tggcctgatt gcgatcgtcg ccgtggcaga tgagtttggc
     5221 gatcatcgaa tcgtagtgca tcgatatttc gccaccctca tatacgccgg tgtccacacg
     5281 cacctcgttt tcgacggctt ccggcggctg gaatctgatc agtcgcccgg tcgaaggcat
     5341 gaaaccccgg aagggatctt cggcattgat tcgacactcg atggcccagc cctttggctt
     5401 gatatcggct tgagcaaagg ggagtttttc gccagcagcg atccggatca tcaattcgac
     5461 aatgtcgagc ccggtgatca tctcggtcac cggatgttcg acctgcaagc gcgtgttcat
     5521 ttcgaggaag tagaacgatt tgtccgagcc gacaacgaac tcgacggttc ctgcgctctg
     5581 gtagtcgact gctttggcca gcgcgactgc ctgctcgccc atcgctttcc tgatcgcgtc
     5641 atcgatgaat ggcgacggcg cttcctctat gactttctgg tggcggcgct gaatcgaaca
     5701 ctcccgctca agaagataaa cggtgttgcc atgggcgtca gcgatgatct ggatttcgat
     5761 gtggtgcggt ccctcaacaa acttctcaat gaagacgcga tcgtcgccaa agctgttgcg
     5821 cgcttcattg cggcaggaat caaatccttc tgaggcctca gtatcgttga aggcaacgcg
     5881 cagccctttg ccgccaccgc cggccgaggc cttgatcatc accgggtaac cgattccctt
     5941 ggcgatctcg accgcttgtt tggcgtcagc gatggcgtcg ttgtaaccgg gaatggtgtt
     6001 gaccttggcc ttcagggcga gtttcttcga ttcaatcttg tcgcccattg cagcgacgga
     6061 gtggtgcttg gggccgataa aggtaatacc gttctcttca aggcgcttcg agaactcctg
     6121 attctcggag agaaaaccgt aaccgggatg gacagcttca gcgccggttt gcttgcaggc
     6181 agcaataata ttgtcaatct taaggtacga gtctttcgaa gccgctggcc caatgcagac
     6241 ggcttcgtcc gcactcagta catgcaggga atccttgtct gcttcagagt gtacggcgac
     6301 ggtagcgatg cccatgcggc gggcggtttt gatgacgcgg caggcgattt caccgcgatt
     6361 ggcgatcaga attttcttga acatttttta acctctttca acgaagcaat ccccttgggg
     6421 ggcagaatcc gcagaaacca tggatagcgc ggacaatgca gtgaaatgcc gggacagggt
     6481 tcgtgtggat ctctgtgcct cttgcgctca ctgtattgac tgcgtgccgg gtatttacag
     6541 tggaatattc ccgtgcttgc gccagggatt ggtcaactgc ttgtcgtgca acatggcgag
     6601 cgaacggcaa atccgtttac gcgtttcgtt cggcatgatg acatcgtcaa tgaacccgcg
     6661 tgctccggcg acgaaagggt t
//
LOCUS       DAORPM010000125         6681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3011_length_6681_cov_6.921069, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000125 DAORPM010000000
VERSION     DAORPM010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3011_length_6681_cov_6.921069"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..671
                     /locus_tag="PLD53_07280"
     CDS             159..671
                     /locus_tag="PLD53_07280"
                     /inference="COORDINATES: protein motif:HMM:NF040080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HRC60752.1"
                     /translation="MSPSEKVPKAMTDKFAAITTLVDAFCTQHLNEEYRQIIHHVVGA
                     LARKRPSPLSKGKENVWAAAAVHAVGRVNFLDDPSQTPHCKSKAIYDFFGIGESTGQN
                     KSKEIRDLLKMGPMSPEWTLPNRLADNPMVWLLQVNGIAVDIRHASIELQRLAFEKGL
                     IPFIPAERDM"
     gene            683..1669
                     /locus_tag="PLD53_07285"
     CDS             683..1669
                     /locus_tag="PLD53_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HRC60753.1"
                     /translation="MPDLDPTPHSSPLTQSVTRDGKSVQIDIYRDGEGGWILEVVDEY
                     GNSTVWDDSFATDQDALDEALKTIAEDGIDSLIGTSSEGHGMQELDQPLSADELDELD
                     DFLANESIEDTSMDVSTLEGFLTAIAIGPRTVMPSEWLPWVWDMEDGESEAGFESVEQ
                     ANRIISLVMRYYNAVVHAFMDDPASFEPVFWSGDQWGAAEWCEGFLLGIQFSDEAWSL
                     LAAGQPTWFTPFLRLGTDDGIDITKRAGDAEKWINEIELSLVKMHAYWKDSRASQPGG
                     LVDDDFHFGGQQEVGQVIRGGPKIGRNDPCPCGSGKKFKKCCGANDAPRTFH"
     gene            1834..2766
                     /gene="cysK"
                     /locus_tag="PLD53_07290"
     CDS             1834..2766
                     /gene="cysK"
                     /locus_tag="PLD53_07290"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRC60754.1"
                     /translation="MRIANSVTELIGNTPLVRINRLAEGAGATIAAKLEFFNPAHSVK
                     DRIGESMITAAEKAGLIKPDTLLVEPTSGNTGIALAMVAAARGYKIVLTMPETMSKER
                     RALLRAFGAELILTPGPDGMGGAIKKAEELAASDPRYLLLQQFNNPANPDVHRRTTAE
                     EIWRDTDGKVDILISGVGTGGTITGVGEVIKERKPSFQAIAVEPDASPILSGGAKGPH
                     PIQGIGAGFVPKVLNTRIYDEVIRVKNDDAFDFARRAAREEGLLVGISSGAALWAAVE
                     VAKRPENAGKLIVVIIPSFGERYLSTPLFAGLVD"
     gene            3230..3718
                     /locus_tag="PLD53_07295"
     CDS             3230..3718
                     /locus_tag="PLD53_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRC60755.1"
                     /translation="MADDHYSSAPASAGPTEKPAHAIDPEFLRGLREMAETIFPKQCR
                     SCGNVYSDSRDFIERTVGIGPKKGVKTGFDDDDTRVLEFFRNCACGSTLHGLYAERRD
                     YSENGERQREKFDSLLSQLVGHGIDRGLARLELRKLLRGEESPVLAPFLGHERKDIDL
                     AP"
     gene            complement(3744..4373)
                     /locus_tag="PLD53_07300"
     CDS             complement(3744..4373)
                     /locus_tag="PLD53_07300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC60756.1"
                     /translation="MAAPLDIEKIARLTLTHYNQRADEFRDGTRDHDVSQNINALLRH
                     IGGAPPFTILDFGCGPGRDLKVFAGLGHRVIGLDGAERFVRMACAETGCEVWHQDFLG
                     LDLPEAFFDGVFANASLFHVPTQELPRVLIQLRDALKIGGVLFASNPHGKNVEGWNGG
                     RYGAFHDPERWRFFMTRAGFAELEHYYRPAGLPRAQQPWLASVWRRLAS"
     gene            complement(4379..4729)
                     /locus_tag="PLD53_07305"
     CDS             complement(4379..4729)
                     /locus_tag="PLD53_07305"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRC60757.1"
                     /translation="MNTSNHDLKTKHCKPCEGGVPPLDRQEIDHLLKDLAGWILVGNE
                     LVKAFDFSNYYETMAFVNATAWVSHQEDHHPDLEVGYRQCRVRYSTHAIGGLSENDFI
                     CAAKIDALRTSASE"
     gene            complement(4743..5402)
                     /locus_tag="PLD53_07310"
     CDS             complement(4743..5402)
                     /locus_tag="PLD53_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HRC60758.1"
                     /translation="MSHELPLLTPLEARILGVLVEKEKTTPDAYPLTLNSLTSGCNQK
                     TSRDPVMNVGESEIQAALEELRSRVLVLETYGASGRVLRYAQNFGKCYGLPAASVALL
                     TALMLRGAQTVSELRANCERLYRFNDASSVEAYLDELAALASGALVTRLPKQPGSREH
                     RWAHLLCGPPSLPDHQTIGNDSGVNNTADLEERVSRLEIEVGELRNLIGRIGVDATGQ
                     S"
     gene            5542..>6681
                     /locus_tag="PLD53_07315"
     CDS             5542..>6681
                     /locus_tag="PLD53_07315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC60759.1"
                     /translation="MADPQYSACPMSDLTITPRHSAEAIPRDVWARLCPPCHPFLNAD
                     FLEILERHGAAGRKWGWVARHLVASDEQGIVVGVLPLYVRFNSHGDFIHDWSWASAYE
                     QLGRAYYPKLLSGLPHTPASGPRMLVDGSLDASSASAIRRALIDGAMALAVDYNASSW
                     HVAFPGEQDYTALQDTGLLTSHNVQFHWGNEGDGTYDDFDGYLATFSAEKRRKVRAER
                     RRVSESGLRIEVRHGDEIDPEEWPALHALYASTFEKFNNIAVLSSACLADLALALRRH
                     MVVFMAFDRGRLVAMSLCFRSDETLYGRYWGCSGNYHSLHFELCFYQGIAYCLRDGLR
                     RFEPGAGGEHKLARGFTPTVVRSSHWIADPAMRELIGHHLQRQGVM"
BASE COUNT         1425 a         1979 c         1946 g         1331 t
ORIGIN      
        1 gccaacctgt ccgaaggacg ctgccgattt gtaatggtca aggagaagcg gtgggactgg
       61 atcgaagagc agttggcgtg aacggggatg aaggaatacc gatttgtttg aataaccgtg
      121 ttgccatcgc catccggcaa tgtttgtgag gtaaggaaat gtcaccgtct gaaaaagtcc
      181 ccaaagccat gaccgacaag ttcgcggcca tcaccacgct tgtcgatgcc ttttgcaccc
      241 agcacctcaa cgaggaatat cggcagataa tccaccatgt cgtcggtgct ctggccagaa
      301 agcggccctc gccgctatca aagggcaagg agaacgtgtg ggccgctgct gcggtgcacg
      361 ctgtcggacg ggtcaatttt ctcgatgacc cgtcccaaac acctcactgc aaatcgaagg
      421 caatctacga tttttttggt atcggggaga gcaccggaca gaacaagtcg aaggaaatcc
      481 gcgacctact gaaaatgggt ccgatgtctc cggaatggac cttgcccaac cggctcgccg
      541 ataacccgat ggtgtggctg ctgcaggtca acggtattgc ggtcgacatt cgtcacgcgt
      601 cgatcgaatt acagcgtctc gccttcgaga agggactgat tccgtttatt cccgctgagc
      661 gggatatgta gggagacgcg caatgcctga ccttgatcca accccacatt cgtcaccgct
      721 cacgcaatct gtcacgcggg atggcaagtc ggttcaaatc gatatctaca gagacggcga
      781 aggtggctgg attctggaag ttgtcgatga gtacgggaac tccaccgttt gggacgattc
      841 gttcgcgacc gaccaggacg cgctcgacga agcgctgaaa acgattgctg aggatgggat
      901 tgactcactg atcggcacat catccgaagg acatggaatg caggaactgg atcagccact
      961 ttcagcggac gaactcgacg agctggacga ttttcttgcc aacgagtcca ttgaagatac
     1021 gtccatggat gtttcgacac tggaaggctt tctgacggcg attgccattg gaccgcgcac
     1081 cgtaatgccg tccgagtggc tgccgtgggt gtgggatatg gaagacggcg agtccgaggc
     1141 gggattcgag agtgtcgaac aggccaaccg gatcatctcg ctcgtcatgc gatactacaa
     1201 cgccgtggtg cacgcgttca tggatgatcc ggcatccttc gaaccggttt tctggtctgg
     1261 cgaccaatgg ggcgccgccg aatggtgcga gggtttcctg ctcggcatcc agttcagcga
     1321 cgaggcgtgg agcctgttgg ccgctggcca gcccacatgg tttacgccct tcctgcgttt
     1381 gggcaccgac gatggcatcg atatcaccaa gcgcgcgggc gatgcggaga aatggatcaa
     1441 cgaaatcgaa ctgtcgctgg tgaaaatgca tgcctactgg aaagacagtc gagccagcca
     1501 gccgggtggc ctggttgacg atgattttca cttcggcgga cagcaagaag tcgggcaggt
     1561 gattcgaggc gggccaaaga tcggccgcaa cgatccgtgc ccctgcggta gcggcaagaa
     1621 attcaaaaag tgctgcggag cgaacgatgc tccgcgcact tttcattgag aatcatgcga
     1681 tgaagaggag gtgcgcggct aaccttcaga tatcaacgat ctcgggagta ttattaccac
     1741 taccgtctcg ggcctcgacg gaaaatgacc ttcggcacgt acgagccaca accgaatcca
     1801 agcacccctc acgaacctga caggagaacc accatgcgca tcgccaacag cgtcaccgaa
     1861 ctgatcggca atacgccgct tgtccgcatc aatcgactgg ccgaaggcgc cggcgcgacg
     1921 atcgccgcca agctggagtt ttttaatccg gcgcacagcg tcaaggatcg catcggcgag
     1981 tcgatgatca ccgccgccga aaaggctggc ctgatcaagc cggacaccct cctcgtcgag
     2041 cccaccagcg gcaacaccgg tatcgcactg gccatggtcg ctgccgcgcg aggttacaag
     2101 atcgtgctga ccatgccgga aaccatgagc aaggaacggc gcgccctgct acgcgccttc
     2161 ggcgccgaat tgatcctgac cccagggccg gacggcatgg gcggtgcgat caagaaagcg
     2221 gaggaactgg cggcgtccga cccgcgctac ctgctgctgc aacagttcaa taacccggcc
     2281 aaccccgacg tccaccgccg cacgacggcc gaagaaatct ggcgcgatac cgatggcaag
     2341 gtggacatcc tgatttccgg cgtcggcacc ggcggcacga ttaccggcgt tggcgaggtg
     2401 atcaaggaac gcaaaccctc gttccaggct atcgccgtag aaccggatgc ttcgccgata
     2461 ctttcgggcg gcgccaaagg accgcacccg atccagggta tcggcgccgg cttcgtgccc
     2521 aaagttctca acaccaggat ttacgacgaa gtgatccgcg tcaagaatga cgatgcattc
     2581 gattttgccc gacgcgccgc tcgcgaggaa ggactgctcg tcggaatttc atcaggtgct
     2641 gccctgtggg cggccgtgga agttgccaaa cggcctgaaa acgcgggcaa gctgatcgtc
     2701 gttatcattc cctcattcgg ggaacgctac ctcagcacac cgctttttgc gggtctggtg
     2761 gattgacaat gcggtgactt gcgatgctcc cctgccctgg ataggataat tactcggggc
     2821 ttgggagcct gaagagcaac ggaaggagcg gatcaaaccc cggcgtgttt tgcgcctgca
     2881 gcgttttgcc gatggcgagc aacccagtca accagcaaac ctacgcacgg cgacgacaag
     2941 caggcaacca tgaacacgat ggcaaaacct gcgcccaaaa ccgagattcc cgtttccact
     3001 ttccttctcc ttaaaactcg aaaactactt acctgcaccc aacccgcccg gtacgcgcaa
     3061 accgttgttt ctgaaaggag acaactggta ttcggcgggt ccgaattgac tatatttcgc
     3121 caatgccgtt ccggcaaatg gtcaaatctc atccgacgcc tgagaatatt tcatccattg
     3181 aatattcgat tttttcatct cagcgccgaa aacgcaggac aattccacca tggccgatga
     3241 tcattactcc tcagcacccg catcggccgg acccacggaa aaaccggcgc acgcgatcga
     3301 cccggagttc ctgcgcggct tgcgcgaaat ggcggaaacc atcttcccca agcaatgccg
     3361 gagctgcggc aatgtgtatt ccgattcgcg agattttatc gagcgcacgg tcggtatcgg
     3421 cccgaagaaa ggggtcaaga cgggtttcga cgacgatgac actcgcgtac tggaattctt
     3481 ccgcaattgc gcctgtggat caaccctgca cggtctctat gccgaacgcc gcgattactc
     3541 tgagaatggc gagcgccaac gcgagaagtt tgattcgttg ctaagtcagc tggttggcca
     3601 cggtattgat cgaggattgg cgcgactcga actgcgcaaa ctgctgcgtg gagaggaaag
     3661 cccggtgctt gcgcccttcc tcggccacga aagaaaagat atcgatctgg ccccgtagct
     3721 acgtcattgc ggagtgacga gggtcatgac gccagcctgc gccacacgct ggccagccac
     3781 ggctgctgcg cacgtggcaa accggcgggc cggtagtagt gctcaagctc ggcaaacccg
     3841 gcccgtgtca tgaagaagcg ccaccgttcc gggtcgtgaa aggcgccgta gcgtccgccg
     3901 ttccaacctt cgacattctt gccatgcgga ttggaagcga acagaacgcc accgatcttc
     3961 agtgcgtcac gcagttgaat cagcacgcga ggcaattcct gtgttggcac atgaaacagc
     4021 gacgcgttgg caaacacgcc gtcgaaaaac gcctcgggca gatccaggcc cagaaaatcc
     4081 tgatgccaca cctcgcagcc ggtttcggcg cacgccatcc ggacaaaacg ctcggcgcca
     4141 tccagcccga tgacccggtg accaagtccg gcgaacactt tgaggtcgcg acccggaccg
     4201 caaccaaaat ccagaatcgt gaaaggtggc gcgccgccga tatgccgcaa caaggcgttg
     4261 atgttctgac ttacgtcgtg gtcacgcgtg ccatcgcgga attcgtcggc acgctggttg
     4321 tagtgcgtca atgtcagcct ggcgatcttc tcgatatcca gcggcgctgc catgggtctc
     4381 attcagatgc ggaagttcgc agggcgtcta ttttcgcggc acaaatgaag tcgttttccg
     4441 acaagccacc gatggcatgc gtcgagtagc ggacacggca ttgtctgtag ccgacctcca
     4501 ggtcgggatg atgatcttcc tggtgggaaa cccaggccgt tgcattcacg aaagccatgg
     4561 tttcgtagta gttactgaag tcgaacgctt taaccagttc atttccaacc agtatccagc
     4621 cagccaaatc cttcaatagg tgatcaatct cctgccgatc cagtggcgga acaccgcctt
     4681 cgcagggttt gcagtgtttt gtcttcaagt catggttcga ggtattcatg gtaatttccc
     4741 tatcaggatt gacctgttgc atcgacacca atccggccaa tgaggtttcg aagttcgccg
     4801 acctcgattt caaggcgact caccctttcc tccaggtcgg cagtgttgtt cacgccgcta
     4861 tcgttaccga tagtctggtg gtcgggcagg cttggcggcc cgcagagcag atgcgcccag
     4921 cgatgttcgc gcgatccggg ctgcttcggc aggcgcgtaa ccagcgcgcc actagcaagt
     4981 gccgcaagtt cgtcaagata cgcttcaacg gaggaggcat cgttgaagcg atacagccgc
     5041 tcgcaatttg cgcgcaactc gctgacggtt tgcgcgccac gcaacatcag ggcggtcaac
     5101 aacgctacgc tggcggccgg caagccatag catttgccga agttttgcgc gtagcgcaac
     5161 acccggcccg aagcaccata ggtttcgagc accagcacgc ggctgcgcag ttcctccagc
     5221 gcggcttgta tctcgctttc gccaacattc atcaccggat cgcgtgaggt tttctggttg
     5281 cagcccgagg tcagactgtt caaggtcagt ggataggcat cgggagtggt cttttccttt
     5341 tcgaccagca ccccaaggat gcgggcttcg agcggggtca gcaaaggcag ttcgtggctc
     5401 atgtcggatt ctcatttctc gtgttcgcgg ttctgtcgat ggtcgcattc tccattctcc
     5461 caagcgcgtg ctcgtcatac aagacaaacc taactggctt atcgtcatac attgcgcctt
     5521 gggccgcgcg atgcggcaca gatggccgat ccgcagtatt ctgcctgtcc gatgtccgat
     5581 ctcaccataa ccccccgcca ttccgccgaa gccatcccgc gcgacgtatg ggctcgcctc
     5641 tgtccgccgt gccatccctt cctcaacgcc gattttctgg agatcctcga acggcacggc
     5701 gcggcgggac gcaagtgggg ttgggtggcc cgccatctgg tggccagcga cgaacagggt
     5761 atcgtggtcg gcgtgctgcc gctctacgtc aggttcaatt cgcacggcga tttcatccac
     5821 gactggtcgt gggcgtcggc ctacgagcag ctcgggcggg cgtattaccc caagctgctg
     5881 agcgggctgc cgcatacgcc ggccagcggg ccgcggatgc tggttgacgg cagcctcgat
     5941 gcatcctcag catccgcgat ccgtcgggcg ctgatcgacg gcgcgatggc cctggcggtg
     6001 gactacaacg cctcgtcgtg gcacgtggcg tttccaggtg aacaagacta cacagcgttg
     6061 caagacacag ggctgctgac cagccacaat gtccagttcc actgggggaa cgaaggcgac
     6121 ggcacttacg acgacttcga tggctatctg gcgacttttt cggcggagaa acggcgcaag
     6181 gtcagggccg aacgccggcg cgtcagtgaa agcgggttgc gcatcgaagt ccgccacggc
     6241 gacgaaatcg atccggaaga gtggcctgcc ctgcacgctc tctacgcttc aacgttcgag
     6301 aagttcaaca acatcgcggt gttgtcatcg gcgtgcctgg ccgatctggc actggcattg
     6361 cgccggcaca tggtcgtctt catggccttt gaccgcggcc gcttggttgc catgtcgctc
     6421 tgtttccgca gcgacgagac cttgtatggc cgctactggg gatgcagcgg caactatcac
     6481 agcctgcatt tcgagctgtg tttctaccag ggaatcgcct actgcctgcg tgacggactg
     6541 cggcgtttcg agcccggcgc cggcggcgag cacaagcttg cccgcggctt cacgccgacc
     6601 gtcgtccgtt cgtcgcactg gatcgctgac ccggcaatgc gcgagctgat cggacaccat
     6661 ctgcagcgcc aaggcgtgat g
//
LOCUS       DAORPM010000126         6618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3051_length_6618_cov_9.686881, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000126 DAORPM010000000
VERSION     DAORPM010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_6618_cov_9.686881"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PLD53_07320"
     CDS             complement(<1..1054)
                     /locus_tag="PLD53_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HRC60760.1"
                     /translation="MRVAPKIVLSEEERGELTKLSRSRRASVRLAQRARIVLLAADGM
                     RNDEIADQLGIGRVQVSRWRGRYAKSRLAGIERDLPRGAPPVKVDVARLVELTTQSTP
                     EAATHWSTRKMAAELGVSAASISRHWRANGVKPHLVRGFKVSRDPRFVEKLEDIVGLY
                     MSPPEHALVLCCDEKSQVQALDRTQPGLPLKKGRAATMTHDYKRNGTTTLFAALNVLD
                     GSVIAQCEQQHRHTEWLKFLRKIDRETPKDKALHLIADNYATHKHPVVQEWLAKHPRI
                     QMHFTPTSASWLNMVERFFRDITTERLRRGVFTSVPALVSAIDDYIAHHNTKPKPFIW
                     TKSARDILQKVIRANSR"
     gene            complement(1132..2538)
                     /gene="ccoG"
                     /locus_tag="PLD53_07325"
     CDS             complement(1132..2538)
                     /gene="ccoG"
                     /locus_tag="PLD53_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRC60761.1"
                     /translation="MPQQPAPRKVIKITPVVDPDAKVYPRSISGPFTRWRWIFVWLTQ
                     IVFYGLCWVPWHGRQAILFDLETRKFYFFDLVLWPQDTIYLVLLMVLGALALFLFTAV
                     AGRLWCGYACPQTVYTEIFLWFEKLIEGERPQRIKLDAAPWSLRKLATKTAKHAIWLL
                     FSLWTGFTFVGYFTPIRDLAQDLVTFSAGAWSSFWVVFYGFATYGNAGFLRDQMCRQI
                     CPYARFQFVMFDPDTLIIAYDVERGESRGARARGIDPRTQGLGDCVDCSICVQVCPTG
                     IDIRDGLQNECIGCAACIDACDQVMDKMNYPRGLIRYSTENAVSKRYTPMDIVRRAFR
                     PRVIIYSAILVLLVAATSWSLKTRVPLKVNILKDRNTLSREADDGSIENIYRLQIMNT
                     GDLTRTFRISIAGIENIHLDDDQTITVTGGEIGTRTAIVRTNPGAAKSGANPIRFSVE
                     DIDDPTVAVTELSKFWMP"
     gene            complement(2576..2977)
                     /locus_tag="PLD53_07330"
     CDS             complement(2576..2977)
                     /locus_tag="PLD53_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRC60762.1"
                     /translation="MKRHPELLTLSREHHAALKLARDARRAAKSGEASELGNLAQRVV
                     SFFSAELDPHFRIEEKHLLVFLAHAGERDLVQRTLTEHAELRSIASTLCCPDPAMLLR
                     FADLLAEHVRFEERELFEVAQARLDGNPIQI"
     gene            complement(3011..3181)
                     /locus_tag="PLD53_07335"
     CDS             complement(3011..3181)
                     /locus_tag="PLD53_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60763.1"
                     /translation="MHLIIADLGTAAEALAGNDQKMSRQEGAMRLQNVLKTYGEFFDH
                     PNWRLSTSAHEH"
     gene            complement(3172..3504)
                     /locus_tag="PLD53_07340"
     CDS             complement(3172..3504)
                     /locus_tag="PLD53_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60764.1"
                     /translation="MSYQTRNWLIIAATLALNIGSAFAIDHAHDLEGHGATTLTLNNG
                     TKWPIDAPLRQGMTSIKAALLPQLPAIHENRLKASQYLRLAKQTNTQIGFMVTNCKLS
                     PDTTRNCI"
     gene            3612..4043
                     /locus_tag="PLD53_07345"
     CDS             3612..4043
                     /locus_tag="PLD53_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRC60765.1"
                     /translation="MKLTTFSDYTLRVLMFLALNRDRLATIPEIAAAYDISENHLMKV
                     VHQLALAGVIETLRGKGGGIRLAREPEDIRLGQIIRTSEGSTPIVECLSDGGACRIAP
                     ACRLTAVLVRAFDALFATLDEYTLADLVCAPRSLNALLVRG"
     gene            complement(4357..5349)
                     /locus_tag="PLD53_07350"
     CDS             complement(4357..5349)
                     /locus_tag="PLD53_07350"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRC60766.1"
                     /translation="MARTSAVEYERVAEACSSLFLEGKSPSFESVYELIGRKGGAKVV
                     QGMIADWRKEMANRFMAGRTNPDLPEELITESDKVLTVVWRLALEKSDTAYADERSRL
                     DQARVDMAAAVTQAQERAAEFEREALSLQGERQALQARLQGSQAEIEDLRQRLADTTT
                     LLRARDEQIGQMREDGARLANTLESERRNHEVELLAERDRHEQAVNAERQRAHESVER
                     EREIAAGERQYLMRQTDEIRQAAKANEAVLKEQLQEAKSFAESFQARAGRAESEAAFW
                     RGKAEAAAEESSRWQRASEEAAGKIDGLQGRIRILEHELTERAPEESPTEKAAN"
     gene            complement(5336..5509)
                     /locus_tag="PLD53_07355"
     CDS             complement(5336..5509)
                     /locus_tag="PLD53_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60767.1"
                     /translation="MDTPQAQIIEQEIHDLSGLLCDLYRRVAENAPERAGEIAGALQA
                     NARQFAEIPSGTH"
     gene            complement(5624..5950)
                     /locus_tag="PLD53_07360"
     CDS             complement(5624..5950)
                     /locus_tag="PLD53_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRC60768.1"
                     /translation="MRVRLLDQAKNDLVALGQYYRDVGGDVLARKMVARIKTPVLSLK
                     DNPDIAPSYELAPGIRRLVVANGAFLVFYRVRADVEVLHIRRAERDSATGDGLEQIAI
                     KESTVL"
     gene            complement(5940..6365)
                     /locus_tag="PLD53_07365"
     CDS             complement(5940..6365)
                     /locus_tag="PLD53_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60769.1"
                     /translation="MQNMTLEQFRATVESGGILSVVLKAQGAAFAIQAETQRGDAVLV
                     DTRRKLPRLFGDPRKALALLREMGIRKAAVDTEAWRPEQADSLHPPRPDKSVKLKAAH
                     EAAELKRVLDERIAMADAQGAIWHEAEDVFDELVAAHAG"
BASE COUNT         1269 a         1965 c         1940 g         1444 t
ORIGIN      
        1 agcggctgtt ggcgcgaatg accttctgca ggatgtcgcg agcgctcttg gtccagataa
       61 agggcttggg cttggtgttg tgatgggcga tgtagtcgtc gatggcagag accaacgcgg
      121 ggacgctggt gaacacgcca cggcgcagtc gttcggtcgt gatatcgcgg aagaagcgct
      181 cgaccatgtt cagccaggag gccgaggtgg gggtgaaatg catctgaatc cgcggatgct
      241 tggccaacca ttcctgtaca acgggatgct tgtgtgtggc gtagttgtcg gcgatcaggt
      301 gcagcgcctt gtccttcggc gtttcccggt cgatcttgcg aagaaacttg agccactctg
      361 tgtgacggtg ctgctgttcg cactgtgcga tcacgctgcc gtccagcacg ttgagcgcgg
      421 cgaacagcgt ggtcgtgccg ttgcgcttgt aatcgtgggt catggtggct gcacgccctt
      481 ttttcagcgg cagcccgggc tgcgtacggt cgagtgcctg cacctggctt ttctcatcgc
      541 agcacaacac cagcgcatgc tcgggtggcg acatgtacag accgacgatg tcttcgagtt
      601 tctcgacgaa cctggggtca cgagagacct tgaagccacg taccaggtga ggcttcacgc
      661 cgttggcgcg ccagtggcgc gagatgctgg ccgcactgac gcccagttcg gcggccatct
      721 tgcgggtact ccagtgcgtc gctgcttcgg gtgtgctttg cgtggtcagt tccaccagtc
      781 gcgccacatc caccttcacc ggaggggcgc cgcgcggcaa gtcgcgctcg attcccgcca
      841 gccgcgactt cgcataacgc cctcgccaac gcgacacctg tacgcggcca atccccaact
      901 gatccgcgat ctcatcgttt cgcatcccat cagcggccag cagcacaatc cgcgcacgct
      961 gtgccagccg cacgctggcg cgcctggaac gagaaagctt ggtcaactca ccacgctcct
     1021 cttcggacaa gacaatcttc ggggcaactc gcatctgcat ctccactccg cacacgacgt
     1081 gcattggaga cgtggtatcc agacaagttc cctcaagaat gcaacactac actagggcat
     1141 ccagaacttt gagagttcgg taacggcaac ggtaggatcg tctatatcct cgacactgaa
     1201 cctgatcggg ttggcgccag attttgccgc tccgggattg gtcctgacaa tggcggttcg
     1261 ggtaccgatt tcgccgcctg tcaccgtgat cgtttggtcg tcgtcgaggt gtatgttttc
     1321 aatgccggca atgctgatcc ggaaggtccg ggtcaaatca cctgtgttca tgatctgcag
     1381 tcggtagatg ttttcgattg acccatcgtc agcttcgcgt gagagcgtgt tgcggtcctt
     1441 cagtatattg actttgagcg gtacgcgcgt tttcagtgac caagaggtcg ccgccaccag
     1501 caagacaagg atcgcgctgt agatgatcac tcgtggccga aacgctcgcc gcacgatatc
     1561 catcggcgtg tagcgcttgc tgaccgcgtt ctccgtggag tagcggatca ggccgcgagg
     1621 ataattcatc ttgtccatca cttgatcgca ggcgtctatg caggcggcgc agccaatgca
     1681 ctcattctgc aaaccatccc ggatgtcgat tccggtggga caaacctgga cgcagatgct
     1741 gcagtcgacg caatcgccga ggccctgtgt tcgcgggtca atacctctgg cacgtgcccc
     1801 acgcgactcg cctcgttcga catcgtaggc gatgatcagg gtgtcgggat cgaacatgac
     1861 aaactgaaat cgcgcgtatg ggcaaatctg tcgacacatc tggtcgcgca ggaagccggc
     1921 attgccatac gtggcgaaac catagaagac tacccagaag gatgaccatg cgccagccga
     1981 gaaggtaaca agatcctgcg caagatcgcg aatcggcgtg aaatagccga cgaaagtaaa
     2041 acctgtccac agggagaaga gcagccaaat ggcgtgcttg gccgtcttgg tggctagctt
     2101 gcgtaacgac cacggcgcgg catcgagctt gatgcgctgc ggccgttcac cttcgatgag
     2161 tttctcgaac cacaggaaaa tttccgtata caccgtttgc ggacaggcat agccgcacca
     2221 caggcgtccc gctaccgcgg taaacaggaa cagggcgagc gctccgagaa ccatcagcag
     2281 aacgagatag atcgtgtcct gcggccacag taccagatcg aagaagtaga atttgcgcgt
     2341 ctcgagatcg aacaggatgg cctgccgacc gtgccatggc acccagcaaa ggccgtagaa
     2401 aacgatctgc gtcagccaga caaaaatcca gcgccagcga gtgaatggcc cgctgatgga
     2461 gcgcgggtaa accttggcat ccggatcaac gaccggtgtg atcttgatga cttttctggg
     2521 ggctggctgc tggggcattt tttttccgac ggccgcaatc cgaatggatt gcgcctcaaa
     2581 tttgaattgg attcccatcg agcctggcct gcgccacctc gaatagttcg cgctcctcaa
     2641 agcggacatg ttccgccagc agatctgcaa atcgcaacaa catggctgga tcggggcaac
     2701 acagtgtgct ggcgatactg cgcaattctg catgttcagt cagcgtgcgc tgaaccaggt
     2761 cacgttctcc ggcatgcgcc agaaacacca gaaggtgctt ctcctcgata cggaagtgcg
     2821 gatcgagttc ggccgagaag aaactgacga cgcgttgtgc cagattcccc agttcgctcg
     2881 cctcgccaga tttcgcggca cgtcgggcgt cacgcgcgag cttgagcgcg gcgtgatgct
     2941 cacgggaaag cgttagcaac tccggatgcc gcttcatgct tgagatctgt ccgccaaatg
     3001 atgtcgccga tcagtgttca tgcgcggaag tggacagcct ccagttggga tgatcgaaga
     3061 attcgccgta ggttttaaga acgttctgca gccgcattgc gccctcctgg cgcgacatct
     3121 tctggtcgtt tcccgccaag gcttcggcgg cggtgccaag atcggcaatg atcagatgca
     3181 attgcgcgtt gtgtcaggcg aaagcttgca attggtgacc ataaagccaa tctgcgtgtt
     3241 cgtctgtttt gccaatcgca ggtattgaga agccttcagc ctgttttcgt gaatcgcagg
     3301 taattgcggt agcagtgcgg ccttgatact cgtcattccc tggcgcaatg gagcgtcgat
     3361 cggccacttc gtgccgttgt tcaatgtcag cgtggtggca ccgtggcctt cgaggtcatg
     3421 cgcgtggtcg atggcgaagg ccgaaccgat attcagcgct agagtggcgg cgatgatcag
     3481 ccagttgcga gtttggtatg acatggcgaa cgtccttgag aaagcggtat aattaatatt
     3541 gcttcatcgt aacaccctct ttttcgtaaa gcaatatagt ttatacagct tatgatcgct
     3601 cacacgctgg cgtgaaactc actaccttct ctgactacac cctgcgcgtc ctcatgttcc
     3661 tggcgctcaa tcgtgaccgg ctcgccacca ttccggagat cgccgccgcc tacgacatct
     3721 cggagaatca cctgatgaag gtggtgcatc aactggcgct ggcaggggtc atcgaaactt
     3781 tgcgagggaa aggtggcggc atccgcctgg cgcgcgagcc tgaggacatt cggctggggc
     3841 agatcattcg caccagcgaa ggcagtacgc ccatcgtcga atgcctgtcc gacggcggtg
     3901 cctgtcgcat cgcaccggct tgccgactga cggcagtact ggtccgcgcc tttgatgcgc
     3961 tcttcgcgac gctggacgaa tacacgctgg ccgatctagt gtgtgcgccg cgcagcttga
     4021 atgcattgct ggtccgcggc taaccaggaa gtggcaaccc ggcagatcat tatgttctgg
     4081 ctgatggcac cttgagtgtg aagcaggttt ccaagccgct ggagcggttt cggcgagttc
     4141 gcacgcaaaa caccgtattt ttctggcgcg ctcatcttgg ttgttggcct ttacctccgc
     4201 gctggcctga ttggtagcag gctatcgagg aagcattgcg acgggttccc ttgtccatcc
     4261 ttggtcattt gcagtgtgcc gctggccagg tacggcacct gttggagaac gatcacgccc
     4321 gtgcctggga agaactcaac ccggaatagt cgctgctcag ttcgccgcct tttccgtagg
     4381 agactcctct ggtgctcgct cggtgagttc gtgctccagg atccgtatgc gcccctgcaa
     4441 gccatcgatt ttcccggccg cttcctctga cgctcgctgc cagcggctgg actcttccgc
     4501 cgccgcttcg gccttgccgc gccagaacgc cgcttcgctt tctgcccggc cggctcgcgc
     4561 ctggaagctc tcggcgaagg atttggcctc ctggagttgt tccttgagca cggcttcatt
     4621 cgcctttgca gcttgccgga tctcatcggt ctggcgcatc aggtactgac gttcgccggc
     4681 ggcaatttcc cgctcgcgct cgaccgattc gtgcgcccgc tgtcgttcgg cgttcaccgc
     4741 ttgctcgtgt cggtcacgtt cggcgaggag ttccacctcg tgattacgtc gctcactctc
     4801 cagggtgttg gcaagcctgg cgccatcttc gcgcatctgg ccgatctgct cgtcacgggc
     4861 gcggaggagg gtggtggtgt cggccaggcg ttgccgcaag tcttcgatct cggcctggct
     4921 tccctggagt ctcgcctgaa gggcttgccg ctctccctgc aaggaaaggg cttcgcgctc
     4981 gaactcggca gcgcgttcct gggcttgcgt caccgctgca gccatgtcga cgcgagcctg
     5041 gtcgagtctg cttcgttcat cggcgtacgc ggtatcggac ttttccagcg ccaggcgcca
     5101 gacgacggtg agcaccttgt cgctctcagt gatcaactct tcggggagat cggggttggt
     5161 gcggccggcc atgaaacggt tggccatctc cttgcgccag tcggcgatca tcccctggac
     5221 gaccttggcg ccacccttgc ggccaatcag ctcgtagacc gattcgaacg acggactctt
     5281 gccttcgaga aagagactgg agcaggcctc ggcgacccgc tcgtactcga ctgcgctagt
     5341 gcgtgccact ggggatctcg gcaaactgcc gggcgttggc ctgcagcgcc ccggcgatct
     5401 cgccagcacg ctccggtgca ttctcggcaa cgcgacggta cagatcgcac agcaagccgc
     5461 tgaggtcgtg gatttcctgc tcgatgatct gtgcctgcgg cgtatccata tcccgtctcc
     5521 ttgaatagca gtgcccaacg ctcgggcagc ggtacttgcc cggcccctca ccgaggatag
     5581 tctattggcg atatatcgtc aaatagcaga cgtatggcgc tttttacagg acggttgatt
     5641 ccttgatggc gatctgctcc aatccatctc ctgttgccga atcccgctcg gcgcggcgaa
     5701 tgtgcagtac ttcgacatcg gcacgcacgc ggtagaacac caggaacgcg ccgttcgcga
     5761 caaccaggcg gcgaatgcct ggcgcgagtt catacgatgg agcaatgtct ggattgtcct
     5821 tgagcgacag cactggcgtc ttgatccgtg ccaccatctt gcgcgccagc acatcaccgc
     5881 caacgtcccg ataatattgc ccaagcgcca ccaagtcgtt cttggcctgg tcgagcaacc
     5941 taacccgcat gagccgctac caattcgtca aagacgtcct ctgcttcatg ccagatggcc
     6001 ccttgcgcgt cggccatcgc tatccgttcg tcgaggacgc gcttgagttc agcagcttcg
     6061 tgcgcggctt tcagcttcac cgacttgtct ggccggggcg gatgcaagga atcggcctgc
     6121 tccggtcgcc aagcttcggt atcgacagcc gcctttctga tgcccatctc gcgcagcagg
     6181 gccagcgcct tgcgcggatc accgaacagg cgcggcaatt tcctgcgcgt atcgaccaga
     6241 acagcatcgc cgcgctgcgt ttctgcctgg atggcgaagg ccgctccctg cgccttgagg
     6301 acaactgaca ggataccgcc ggattccacc gtggcgcgga actgctctag tgtcatattt
     6361 tgcataagtc acctcatcgt ataacagcct gattgcagta tgcgctataa cgcttcacaa
     6421 acaataattg tttgtgtcac tcggttcgac aaacacctga taggggtcgc ctcagaaaac
     6481 ggaaaataaa gcgcgctaac agctggcgtg tcaggttgaa acctgagaag tcgggccgag
     6541 agtgtcagac aaggccgaaa actgccctaa tctgacgcaa tcaaggaacg catctgacgt
     6601 cagattgacg tgtacttc
//
LOCUS       DAORPM010000127         6601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3055_length_6601_cov_15.149099, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000127 DAORPM010000000
VERSION     DAORPM010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3055_length_6601_cov_15.149099"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..882)
                     /locus_tag="PLD53_07370"
     CDS             complement(109..882)
                     /locus_tag="PLD53_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HRC60770.1"
                     /translation="MKNAHNIGGILFDLDGVLYVGNQVIDGAYDAIRAVQERHIPHRY
                     VTNTTTQARDALAHKLQALGFPIEPKHILSAPAAAQVYLRHRQFRSCHFLVADAIREE
                     FHEFPTSQTAPEAVVIGDIGRAWTYDLVNQVFQMVMAGAELIALHKAKYWQTPEGLQV
                     DIGAFVAAVEYATGKEATVIGKPSPAFFQAAVQELDCPKDEIVMIGDDIDADVGGAQK
                     CGIRGILVRTGKYRQAYVSASAITPDAVLDSIAQLPRYL"
     gene            complement(966..1802)
                     /locus_tag="PLD53_07375"
     CDS             complement(966..1802)
                     /locus_tag="PLD53_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HRC60771.1"
                     /translation="MTPELRDKLIDGLKNGSIVPYLGPGVLADVTNATTGAPIPADSN
                     SLILAMNDGRPMSPKLMYEFPRAAMNVELKRGRSTVTRFLTRTYGDTLWTRGAVHDWI
                     RAIHPPYVIDINRDTQLQDSYARLKHNLIVGIARLGGTDFRYKLYYWDTVAYHKSDVI
                     DPNLPILFKPMGTPKPAPDYVASDADYVDYITELMGGFSIPPVVKALRKGKQYLLMGL
                     RLNRDTERMVMSDIIYDAAQPAGWAMIQEPTDKERRYCKKLGLELIESDVFELIGAES
                     FA"
     gene            complement(1944..2852)
                     /locus_tag="PLD53_07380"
     CDS             complement(1944..2852)
                     /locus_tag="PLD53_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HRC60772.1"
                     /translation="MKSENMTVPDAAQTCDKSHVLCRGDDKASLPRDARLPINRCNLP
                     AVVLGSLSYQKYPIPLLLDGVAELHRDLFDRLNATPPEALGAVFADYLTVHFRLERPE
                     EMGLRAGFGGDRKNRAKANYIRMIRGWSFDSDSREGAVLKGWVESRFGLPPRYHGQAL
                     RDFSGEAYRRYLEMRSHGLYGTGALEAQFDLVYAFCQLQLALGYPPQNPPQTHLTLYR
                     GVNRMSDHEVLSKGEGGRHIILLNNLNSFSRSRERAGEFGDYIMTADIPLTKIFFHCG
                     LLPGVLKGEDEYLVIGGVVEVTLSTL"
     gene            2969..3859
                     /gene="nifH"
                     /locus_tag="PLD53_07385"
     CDS             2969..3859
                     /gene="nifH"
                     /locus_tag="PLD53_07385"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HRC60773.1"
                     /translation="MAKLRQCAIYGKGGIGKSTTTQNLVAALAESGKNVLIVGCDPKA
                     DSTRLILHSKAQTTVMHLAAEAGSVEDLELEDVLSVGFGGIKCVESGGPEPGVGCAGR
                     GVITAINFLEEEGAYTDDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAM
                     YAANNICKGIVKYANSGGVRLAGLICNSRNTDREDELVMALAERLGTTMIHFVPRDNA
                     VQHAEIRRMTCVEFDPTHKQTDEYRALASKIVNNTNFVIPTPIEMEELEELLMEFGIM
                     SVEDQTIIGKTAAELAAEAA"
     gene            4009..5481
                     /gene="nifD"
                     /locus_tag="PLD53_07390"
     CDS             4009..5481
                     /gene="nifD"
                     /locus_tag="PLD53_07390"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HRC60774.1"
                     /translation="MTTLSREETEALIAEVLEVYPEKAKKDRSKHLAVNDQSIEQSKK
                     CITSNRKSLPGVMTVRGCAYAGSRGVVWGPIKDMIHISHGPIGCGQYSRAGRRNYYVG
                     TTGVDTFSTMNFTSDFQEKDIVFGGDKKLAKLIEEVDQLFPLNKGISVQSECPIGLIG
                     DDIESVSKKASANLGKPVVPVRCEGFRGVSQSLGHHIANDTIRDWVLDKRDGNTFEST
                     PYDVSIIGDYNIGGDAWSSRILLEEMGLRVVSQWSGDGTIAEMMNTPKVKLNLLHCYR
                     SMNYISRHMEEKYGIPYLEYNFFGPTKIIESLRKIAAFFDDSIKEKCEAVVARYQPMM
                     DAIIAKYKPRLQGKKVMLYVGGLRPRHIIGAYEDLGMEVVGTGYEFAHNDDYDRTIKE
                     MGSATLLYDDVTGYELEEFVKKIRPDMVGSGIKEKYIYQKMGIPFRQMHSWDYSGPYH
                     GYDGFAIFARDMDIALSNPVWGNLVPPWKKAVTEEVQKAA"
     gene            5591..>6601
                     /gene="nifK"
                     /locus_tag="PLD53_07395"
     CDS             5591..>6601
                     /gene="nifK"
                     /locus_tag="PLD53_07395"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HRC60775.1"
                     /translation="MQTVENIKPSYPLFSDDDYKKSIADKRELFEEGHSPEKVRETFM
                     WTTTMEYQELNFKRSALTVDPAKACQPLGAVLCASGFHKTLPYVHGSQGCVAYFRTYF
                     NRHFKEPCSCVSDSMTEDAAVFGGQKNMYDGMANAKALYKPEMIAVSTTCMAEVIGDD
                     LNAFINNTKKEGHIPQEFPVPFAHTPSFVGSHTTGWDNMFEGIIRSFTLNTMEGKKPG
                     SNGKINIVPGFETYLGNYRVIKRMLGEMDVDFTLLCDPSEVFDTPTDGEFRMYAGGTT
                     QDEIKDAPNAYTTVLLHPAQLDKSRKFIENTWNHEVPKLNIPMGVEWTDDFLMKVSEV
                     TGK"
BASE COUNT         1474 a         1948 c         1888 g         1291 t
ORIGIN      
        1 ggacagtggt cgtcccatta ttccggtcaa aatcgcgaaa aatggtcgtt caacgctgcg
       61 catcccaatc ttctcagaaa ttcgtgtctt caccttccgc gcgaactgtc acagataccg
      121 tggcagctgg gcaatcgagt ccagaacagc atcaggagta atggcggacg cgctcacata
      181 cgcctgccga tattttcccg tccgaaccag aattccccgt attccgcact tttgtgcccc
      241 tcccacatcc gcgtcgatat cgtccccgat catcacgatc tcgtccttgg ggcaatcgag
      301 ttcttgcaca gcggcctgaa aaaaagcggg agaaggtttc ccgataacgg tggcctcttt
      361 cccggtggcg tattccactg ccgcaacgaa ggcgccgatg tccacctgga gtccttccgg
      421 tgtttgccag tatttggcct tgtggagcgc aatcaactcc gcgcctgcca tgaccatctg
      481 aaacacctga ttcaccaggt catacgtcca ggcgcggccg atatctccga tcacaaccgc
      541 ctccggagcg gtctgcgacg tgggaaattc gtggaattcc tcccggatgg catcggccac
      601 caggaaatgg cacgaacgga actgtcgatg acgcaagtac acctgcgccg cagcgggagc
      661 actcaagatg tgcttcggtt cgatcgggaa tcccagggcc tgcaatttgt gcgctagcgc
      721 gtcccgcgct tgagtggtgg tattggtcac gtagcggtgc gggatatgcc gctcctggac
      781 ggcgcgtatc gcgtcgtatg cgccgtcaat gacctgattg ccgacataca acacgccgtc
      841 aaggtcgaac aggattccgc caatattgtg tgcattcttc atccgttgcc tcgccgtcgt
      901 gccgatcacc aactgcgcga aaccatcgct tgcctcacat actattgtgt ctcgatcagg
      961 ccgtgtcaag caaaggattc agcaccgatc agttcgaaaa cgtcggactc gatcagttcc
     1021 agccccagct ttttgcagta gcgtcgttcc ttatcggtcg gttcctgaat catggcccag
     1081 ccagccggct gcgcggcgtc gtaaatgatg tcggacatca ccatgcgctc ggtgtcgcgg
     1141 ttcagacgca gacccatcag caggtactgc ttgcctttgc gcaacgcctt gacgacgggc
     1201 ggaatcgaaa agccgcccat cagctcggta atgtaatcga catagtcggc gtcggaagca
     1261 acgtagtccg gcgcgggctt cggtgtgccc atgggcttga acaggatcgg cagattcggg
     1321 tcgatgacat cggatttgtg gtaggccacg gtatcccagt aatacagctt gtagcgaaaa
     1381 tcggtcccgc caagacgggc aatgccgacg atcaggttgt gtttcaggcg ggcgtaggaa
     1441 tcctggagct gcgtgtcgcg gttgatgtca atcacgtagg gcgggtggat tgccctgatc
     1501 cagtcatgga ctgcgccgcg cgtccacagc gtgtcgccgt aggtgcgcgt gaggaagcgg
     1561 gttaccgtgc tgcgcccgcg cttgagttcg acattcatcg cggcgcgcgg gaactcgtac
     1621 atcagcttgg gtgacatcgg ccggccatcg ttcatcgcca ggatcagcga attactatcg
     1681 gcagggattg gcgcgccagt cgtagcgttg gtcacgtcgg ccagtacgcc ggggccgaga
     1741 tagggaacga tgctgccgtt tttgaggccg tcgatcagct tgtcgcgcag ttccggggtc
     1801 atgacaattg ctccgatttg aagggatact catcccgagc aagttccacg cctgtcgcca
     1861 atccgcgcat cggcacgggt gtcctgaccc tggcgtcggc gattgtagga aacacgacag
     1921 cgaatgttgc cgcttgcgtg acatcagagg gtcgacaaag tcacctcgac gaccccgcca
     1981 atcaccagat actcgtcctc gcctttcaac acccccggaa gcaggccgca gtggaagaag
     2041 atcttggtca gcgggatgtc ggccgtcatg atgtaatcgc cgaactcgcc ggcgcgctcg
     2101 cggctgcggg aaaaggaatt caggttgttg agcaggatga tgtgacgccc gccttcacct
     2161 ttgctcagca cttcatggtc ggacatgcga ttgacgccgc ggtaaagcgt gagatgggtc
     2221 tgcggcggat tctgcggcgg gtagccgagc gccagctgca actggcagaa cgcatagacg
     2281 aggtcgaatt gcgcctccag cgcgccggtt ccatacaagc catgcgagcg catttcaagg
     2341 tagcggcggt aggcttcgcc ggagaagtcg cgcaaggcct gcccgtggta gcgcggtggc
     2401 agaccgaaac gcgactcgac ccagcccttg agcacggcgc cttcgcggct gtcggaatca
     2461 aaactccagc cgcgaatcat gcgaatgtaa ttggccttgg cgcggttctt ccggtcgcca
     2521 ccgaagcctg ctctcagccc catctcttcc ggccgttcca gccggaagtg caccgtcagg
     2581 taatcggcga agacggcgcc cagcgcttcg ggcggggtgg cgttcaggcg atcgaagagg
     2641 tcccgatgca gttcggcgac gccgtcgagc agcagaggaa tcggatattt ctggtaggac
     2701 aggctaccca gcaccacggc cggcagattg cagcgattga tcggcaaacg cgcatcgcgc
     2761 ggcagcgatg ccttgtcatc acccctacaa aggacgtggg atttgtcgca agtctgcgcc
     2821 gcgtcaggca ccgtcatgtt ttccgatttc attgatattt caatcgcttg cgatgctttc
     2881 gacgacctgg cacgcaacat gctgcaggac aggtgcgact ctgattgttt cattcgcaaa
     2941 tcgactgaca gaataaggag attacagcat ggccaagttg cgtcaatgtg ccatttacgg
     3001 caaaggcggc atcggcaagt ccaccaccac acagaatctg gtcgcggcgc ttgccgaatc
     3061 cggcaaaaat gtattgatcg tcggttgtga cccgaaagcc gactccactc gcctgatcct
     3121 gcatagcaag gctcaaacga ccgtcatgca tctggcggct gaagcaggct cggtggaaga
     3181 cctcgaactc gaggacgttc tctccgttgg cttcggcggc atcaagtgcg ttgaatccgg
     3241 cggtcctgaa cccggagtcg gctgcgccgg gcgcggtgtc attaccgcca tcaacttcct
     3301 cgaagaagag ggtgcctata cggacgacct cgacttcgtt ttctacgacg tgctcggtga
     3361 cgtggtttgc ggcggctttg ccatgccgat ccgcgaaaac aaggcgcagg aaatctatat
     3421 cgtctgctcg ggcgaaatga tggccatgta tgccgccaac aatatctgca agggcatcgt
     3481 gaagtacgcg aactccggcg gtgtgcgtct ggcgggattg atctgcaata gccgcaacac
     3541 cgaccgcgaa gacgaactgg tcatggcctt ggccgaacgt ctgggtacca cgatgatcca
     3601 cttcgttccg cgcgacaacg ccgtgcagca tgcggaaatc cgccgcatga cttgtgttga
     3661 attcgatccg acgcacaagc aaaccgacga ataccgtgct ttggccagca agatagtcaa
     3721 caacaccaac ttcgttatcc cgactccgat cgagatggaa gagctggaag agctgttgat
     3781 ggaattcggc atcatgagcg ttgaagacca gaccatcatc ggcaagacgg cggcagaact
     3841 ggccgctgaa gcagcctgag ccgcacaggt gattggcata aggaacatgc agtaggcagt
     3901 gggcaacaag caataactag gcacgatacg gcggggttgc tagagcgaat ccgccacttt
     3961 caaccagtgc gccggccacg gatgggtgac cgcgtcagga gaaaaaagat gacaacgcta
     4021 tctcgcgaag aaaccgaagc cctgatcgcc gaggttcttg aggtttatcc tgaaaaggcc
     4081 aagaaagacc gcagcaagca tcttgcggtc aatgaccagt cgattgaaca atccaagaag
     4141 tgcatcacgt ccaaccgcaa gtcgctgccc ggcgtgatga ccgtccgcgg ctgtgcctac
     4201 gcgggttccc gcggcgtcgt ctgggggccg atcaaggaca tgatccacat ctcgcacggc
     4261 cccatcggct gtggccagta ttcccgtgcc gggcgccgca actactatgt cggcacgacc
     4321 ggcgtggaca ccttttcgac gatgaacttc acctcggact tccaggaaaa ggacatcgtt
     4381 ttcggcggcg acaagaagct cgccaagctg atcgaggaag tcgatcagct gtttcccctc
     4441 aacaagggca tctccgtcca gtccgagtgt cccatcggtt tgatcggcga cgacatcgaa
     4501 tcggtctcca aaaaggcctc ggccaacctt ggcaagccgg tcgtcccggt gcgctgtgag
     4561 ggtttccgcg gcgtgtccca gtcgctgggc caccatatcg ccaacgacac catccgcgac
     4621 tgggttctcg acaagcgcga cggcaacacc ttcgagtcga cgccctatga cgtctcaatc
     4681 atcggcgact acaacatcgg cggcgatgcc tggtcttccc gcatcctgct tgaggaaatg
     4741 ggtctgcgcg tggtttccca gtggtccggc gacggcacga tagctgaaat gatgaacacg
     4801 ccgaaggtga agctcaacct gctgcactgc tatcgctcga tgaattacat cagccgccac
     4861 atggaagaga agtacggcat tccctacctg gaatacaact tcttcggtcc aaccaagatc
     4921 atcgaatcgc tgcgcaagat cgccgctttc ttcgacgatt cgatcaagga aaagtgcgaa
     4981 gccgttgtcg cccgctatca gccgatgatg gatgccatca tcgccaagta caagccgcgc
     5041 ctgcagggca agaaggtcat gctctatgtc ggcggactga ggccgcgcca catcatcggc
     5101 gcctacgaag acctcggcat ggaagtggtc ggtaccggct acgaattcgc acacaacgac
     5161 gactacgacc gcacgatcaa ggaaatgggc agcgccaccc tgctctatga cgatgtcacc
     5221 ggctacgagc tggaggaatt cgtcaagaag atcagaccgg acatggtcgg ttcaggcatc
     5281 aaggaaaagt acatctacca gaaaatgggc attcccttcc gccagatgca ctcctgggat
     5341 tactccggcc cctatcacgg ctacgacggc ttcgccatct tcgcccgcga catggacatc
     5401 gcactgtcca atccggtctg gggcaacctg gtgccgccct ggaagaaagc ggtaaccgaa
     5461 gaagtgcaga aggccgcctg agttggcgga gatcgcgaag ttggcgaaca ggaacgaata
     5521 ggaacgaaca ggaccatcca tcagcttgta cccgcagttg cagatgagca ctgcgggcaa
     5581 ggagacagca atgcaaaccg tagaaaatat caagcccagt taccccttgt tcagcgatga
     5641 cgactacaag aagtcaatcg ccgataaacg ggaattgttt gaagaaggcc acagccccga
     5701 gaaggtacgc gaaaccttca tgtggacgac cacgatggaa taccaggagc tcaacttcaa
     5761 gcgcagcgcc ctgacggtcg atcccgccaa ggcctgccag ccgctcggcg ccgtgctttg
     5821 cgcgtccggc tttcacaaga ccctgcccta cgtgcatggt tcgcaaggct gtgtcgccta
     5881 ctttcgcacc tacttcaacc gtcacttcaa ggagccgtgc tcgtgcgtct ccgactcgat
     5941 gactgaggat gcagccgtgt tcggcgggca gaagaacatg tacgacggca tggccaacgc
     6001 gaaggcgctc tacaagccgg agatgattgc cgtatccacc acgtgcatgg ccgaagtgat
     6061 tggcgacgac ctcaacgcgt tcatcaacaa caccaagaag gaaggccata ttccccagga
     6121 attcccggtc cccttcgcac acacgccgtc cttcgtcggt tcgcatacga ctggctggga
     6181 caacatgttc gagggcatca ttcgctcctt cacgctgaac accatggagg gaaagaaacc
     6241 cggttccaac ggcaagatca acatcgttcc gggcttcgaa acctacctcg gcaactatcg
     6301 cgtgatcaag cgcatgctcg gcgagatgga tgtcgatttc accctgctgt gcgacccgag
     6361 cgaggtattc gacacaccga ccgacggtga attccgcatg tatgccggcg gcacgacaca
     6421 ggacgaaatc aaggatgcgc ccaacgccta cacgaccgtt ctcctgcatc cggcgcaact
     6481 cgataaatcc agaaagttca tcgagaacac gtggaatcac gaggtgccca agctgaatat
     6541 tccgatgggc gtcgagtgga ccgacgattt cctgatgaag gtttccgaag tcaccggcaa
     6601 g
//
LOCUS       DAORPM010000128         6536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3109_length_6536_cov_7.882117, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000128 DAORPM010000000
VERSION     DAORPM010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3109_length_6536_cov_7.882117"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..1436
                     /gene="ydiK"
                     /locus_tag="PLD53_07400"
     CDS             357..1436
                     /gene="ydiK"
                     /locus_tag="PLD53_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HRC60776.1"
                     /translation="MAEIHRDLTRTMLAILCILLLIATSLWVLRPFLAAAVWAVMIVV
                     STWPLLKSLQLRFGGRRGLAVTVMTLAMLLLLVVPLWLAIDTIASHAEAIVNLARKFA
                     ASGLPQPPAWVGGVPLIGGKLSALWEQLADAGSQGLMAKAEPYAAQSGKWILAKVGGL
                     GFMLLQFLLVVVFSAILYASGEAGASTVLRFGRRLAGERGENSVILAGQAIRGVALGV
                     GVTAIVQTVLGGIGLAIAGVPFAALLSAVMLMLCIAQLGPGLILFPAVGWMFWMGDTG
                     WGSFLLVWSLVVASIDNFLRPVLIKKGADLPLLLIFAGVIGGMLGFGLIGLFIGPVAL
                     AVTYTLFEAWIDDDLTSAPAERQVN"
     gene            1522..2184
                     /locus_tag="PLD53_07405"
     CDS             1522..2184
                     /locus_tag="PLD53_07405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC60777.1"
                     /translation="MGNPKSATQRLAREYADQGDPNGWFEEFYARACGDIGKVYWADL
                     KPNPLLLDWLKERGASDGMRAITIGCGLGDDAEALSRYGYRVTAFDISPSAIAMCRQR
                     YPASTVDYRVADLFDPPSAWLRGFDLVYECNTIQILAGLSRVQALAAISDLAAPGGEI
                     VVSCRSRNAGEQLDSFPLALDRDEIDGFLRAGLTEIHFVAYDDDQEPPVPHFFAVYRR
                     PA"
     gene            2470..3303
                     /locus_tag="PLD53_07410"
     CDS             2470..3303
                     /locus_tag="PLD53_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRC60778.1"
                     /translation="MSDVRSLSDSGIHAATAILERLVSYLPSFAAAIVLVFVGWVVAK
                     TMRALAIRTALLIDTVLPRLGLPAGLSRFRVARGSVVVGTVVYWLVLLFFVTTATQVL
                     GLQAFTDWLSKLIDYLPTLIVGLLILTAGWLMSGFAAELMQATVTGLEPGQSAMLERV
                     VRISILVGAILVGADQIGIRITFLAIFIGAIAITVGGGLALAVGFGARGHVANLIAAH
                     HLRQAFTVGQTLQFAGHEGRLLEITATNLIIETADGRVMLPASLFNEHPLTVKSRGSN
                     G"
     gene            3296..4144
                     /locus_tag="PLD53_07415"
     CDS             3296..4144
                     /locus_tag="PLD53_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRC60779.1"
                     /translation="MAEAGTLTLAFVSSHPVDAAKVLERLSVADAAALFTNLPARAAA
                     PVLEAMLSPAAARILVALDDQAALGLLSATGVQSAVAMLRHVREPRRTTLIEGLSTTT
                     ALASRMLLGYPDDTVGAWADPEAIVVGSDTATGEALARVRGNPGLEANEIYVVDESQR
                     LQGIVGLQVLLRAHETMSLAALMRPPAGVLPVAMPLSGAAAHPAWKQTNIMPVVERGD
                     RFIGVLHAAKLAETLSRGHSGHTDTPDETLSGMLARGYWDTVSGLLRVGLAVLPPAQP
                     VLPGDK"
     gene            4141..5451
                     /locus_tag="PLD53_07420"
     CDS             4141..5451
                     /locus_tag="PLD53_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRC60780.1"
                     /translation="MNTANLNAVAALNRRYLLDFPYEAARQLESMSPDDGAELLASQP
                     LHAVVRAWQVLGPDVARAILERVPEAMARHLLAEAEPIASVAVLAKWDESERERRLTE
                     LDAQVAHELRELLRYPENSAGRLMDPDVSPLHAGLSVGQALERLRRLKRHGLRQLFVV
                     DNEGHLTGRVEIQDLAVAEHARPLREIVLPVLAVVYALDPQEDVVSILERQPITELPV
                     INVDGRFVGIIRHAALLATLEEETSIDIQTMVGASRDERALSSSIFAVRKRLPWLQIN
                     LLTAFLAAAVVGLFEGTIAKFTALAVLLPVVAGQSGNAGAQALAVTMRGLVLREISLR
                     HWPRVVAKEAGVGLINGLAVAATTAIGVYAWSGSLGLVSVISSAMVIAMVIAGIAGAL
                     VPIALSRFGMDPAQSSSIVLTTVTDVAGFFSFLGIATLLADTLA"
     gene            5502..5915
                     /locus_tag="PLD53_07425"
     CDS             5502..5915
                     /locus_tag="PLD53_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60781.1"
                     /translation="MPPMIRHRFCLLSFAFFAQTFASHTALAAEEVSAQKYPDVLSAT
                     VQARGADSFDFDVTVSSPYDSPRRYADAFRAMGRDGTVFGERTLFHDHAGEQPFTRDL
                     YGVKIPPGVRAVVIQARDQKYGYGGKTVEVVLPGR"
     gene            5974..>6536
                     /locus_tag="PLD53_07430"
     CDS             5974..>6536
                     /locus_tag="PLD53_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRC60782.1"
                     /translation="MSIEHAKQLADNGANPRLTGDMELLLAISQDFVGTPAMDETLAN
                     AIQQIMSYMDTEAASIFLLDEIDEKLTCLACAGPVSIKGLTIDRNQGIVGRALAENAV
                     QMVRDVSADGDFSTAVDDHTGFTTRSILCAPLMVKGRMLGALEVINKRKGDGLFDLAD
                     KQFLQVLAGMVSLAVHNAQMTEALVEQ"
BASE COUNT         1132 a         1942 c         2053 g         1409 t
ORIGIN      
        1 ggctggatgg tggaaaattt catgccaatg gcctcctgag tggcaattgt ggatgtctca
       61 agcaaggcag gggaaagatg cattttccgg catgcggtct actcagcaat gctggactct
      121 gcctgctcca tgggatggca cgctcggccc gtcgagtggc cgaaatctcg tgcgaagagt
      181 cgtgcgaact gcggctgacc gataggcgtg gtgtcaatgc cctggaacga cgcgtgcgga
      241 ctcaggcgca agattttagc atgagggggc caagaccaaa aacccgacgc ctgaacacga
      301 gggttccgca tccttgcggt aatatccgtc ttccgtgttc tccagcgaga cctgatatgg
      361 ctgaaatcca ccgcgacttg acccgcacaa tgctcgcaat tctctgcatc ctgttgttga
      421 ttgcgacttc gctatgggtt ctgcgcccgt ttctggccgc cgcggtgtgg gcggtcatga
      481 tcgtcgtttc cacgtggccg ttgctaaagt cactgcaact gcggtttggc ggtcggcgcg
      541 gtctggcggt aaccgtgatg acgctggcca tgcttctgct gctggtcgtg ccgctctggc
      601 tggcgattga caccattgcc agccatgccg aagcgattgt caatctggcc agaaagtttg
      661 ccgcatcggg cttgccgcag ccgcccgcgt gggtgggtgg cgtgccgctg atcggcggca
      721 aactgtcagc cttgtgggag caactggccg atgcgggctc gcagggcctg atggcgaaag
      781 ccgaacctta cgcagctcaa tccggcaagt ggattcttgc caaggtgggt ggtctgggtt
      841 tcatgctgtt gcagtttctg ctcgtcgtgg tcttctccgc aatcctctac gccagtggag
      901 aagcgggagc gtccactgtc ttgcgttttg gccgcaggct tgcgggggag cgtggggaaa
      961 attccgtcat tctggccgga caggccatcc gtggcgtggc tctcggagtg ggcgtcaccg
     1021 cgatcgttca gaccgtactc gggggtattg gtctcgccat tgccggggtt ccgtttgccg
     1081 cgctgctttc ggcggtgatg ctgatgctct gtattgctca actcggccca gggctcattc
     1141 tctttcccgc cgtcggctgg atgttctgga tgggcgacac gggctgggga tcattcctcc
     1201 tggtatggag tctggtcgtc gccagtatcg acaactttct gcgcccggtg ctcatcaaga
     1261 agggcgccga tttgcccctg ctcctcatct ttgccggcgt catcggcggt atgctcggtt
     1321 ttggcctgat cggacttttc atcggtccgg tggcgctggc cgtgacctac actctattcg
     1381 aggcctggat tgacgacgat ctgaccagcg ctccggccga acgacaggtc aactgaaggc
     1441 tggtcaggat cggaagcgat gttcttcgtc tgttgaattg cgatgccttc atcatgtgat
     1501 gagacactag aggagccgca catgggcaat cccaaatccg caacgcaacg tctggccagg
     1561 gaatacgccg accagggaga tccgaacgga tggttcgagg agttctacgc ccgagcctgt
     1621 ggcgacatcg gcaaggtgta ctgggccgac ctgaaaccca accccttgct gctcgactgg
     1681 ttaaaggaac gcggggcgtc ggacggcatg cgcgcgatca cgatcggctg tggtttgggc
     1741 gacgatgccg aggccttgtc gcggtacggt tatcgcgtta ccgctttcga catttccccg
     1801 tcggctatcg ccatgtgccg gcagcgctat cccgcgagca ccgtagacta ccgggtcgcc
     1861 gacctgttcg acccgccttc agcatggttg cgcggattcg atctggttta cgagtgcaac
     1921 acgattcaga ttctggccgg cttgagccgg gttcaggcgc tggccgccat ctcggacctg
     1981 gccgccccag ggggcgaaat cgtggtttcg tgccgtagcc ggaatgcggg cgaacaactt
     2041 gactcatttc ctctggcctt ggaccgggac gagatcgacg gtttcctgcg tgccggtttg
     2101 acggagatac atttcgtagc ctacgacgac gatcaggagc cgccggtacc gcatttcttt
     2161 gcagtctatc ggcgacccgc ttgaaatcct ttccggtacg ggcgtcagga gggtattcat
     2221 gactgaccgt cgccaagccg ttcgggaagt ttggtgcaaa ccttactgaa caaaacgctt
     2281 gtcgagattg tcatttgccg atagaaccaa ctgcgcagtg tgagtagtca gtctgaatca
     2341 gttgcttgac agacatgcgc tgacaaaagg atgctgtatc ggggggcgac ttacgctgga
     2401 tggtttcccc gcatttgtat ttccttttgt atccccattt ctctcgagcc tttcagtgga
     2461 gcacagcgga tgagtgatgt acgtagtttg agcgattcag gtatccacgc cgcgacggcg
     2521 attctcgagc gtctggtgtc gtatcttccg agttttgccg ccgcgattgt gctcgtattc
     2581 gtcggctggg tcgtggcaaa aacgatgcgc gcactggcca tacgcacggc attgctgatt
     2641 gacaccgtgt tgccgcggct gggactgcct gccggtttgt cgcgcttccg cgttgcgcgc
     2701 ggctccgtgg tcgtgggcac tgtggtctat tggctggtgc tcttgttctt cgtcaccacc
     2761 gccacacagg ttctcggatt gcaggcgttc accgactggt tgagcaagct tatcgattat
     2821 ctgccgacac tgatcgtcgg gctgctgatc cttaccgcag gctggttgat gtccggcttt
     2881 gccgccgaac tgatgcaggc gaccgtgacc ggactggagc ctggccaaag cgccatgctg
     2941 gagcgtgtcg tccgtatcag cattctggtg ggcgccatcc tggtcggcgc ggaccagatc
     3001 ggcattcgca tcaccttcct ggccattttt atcggcgcta tcgcgataac ggtcggcgga
     3061 gggttggcgt tggcggtcgg cttcggtgcc cgcgggcatg tggccaacct gattgccgcg
     3121 caccatctgc ggcaggcctt taccgtgggt caaaccctgc agttcgccgg gcacgaagga
     3181 cgcctgctcg agattacggc aaccaacctg attatcgaaa ctgccgacgg gcgagtcatg
     3241 ctgcccgcca gcctgttcaa cgagcacccc ctaaccgtca aatcgcgagg cagcaatggc
     3301 tgaagccgga acacttacgc tggccttcgt ttcttcccac ccggtcgatg cggccaaggt
     3361 gctggaaagg ctttccgtgg ctgatgccgc agcgttgttc acgaatctgc cggcccgcgc
     3421 ggcggcacct gtgctggagg cgatgctgtc gcctgccgcc gcgcgcattc tcgttgctct
     3481 ggacgatcag gctgcacttg gcctgctctc ggctaccgga gtgcagagcg ctgtggcgat
     3541 gctgcgacac gtccgggagc cgcgccgaac aacactcatc gaagggctgt ccaccacgac
     3601 ggcactggct tcgcgaatgc tgctcggcta tcccgatgat acggtcggcg cgtgggccga
     3661 cccggaagcc attgttgtgg ggtcggacac ggcaacaggc gaagcgctgg cccgagtgcg
     3721 cggcaacccc ggtctggagg ccaacgaaat atacgtggtg gatgaaagcc agcggctgca
     3781 gggaatcgtc ggattgcagg tgctgctgcg cgcgcacgag acgatgtcat tggccgcgct
     3841 gatgcgtccg cctgccggcg tactgccggt cgccatgccg ctctctggag ccgccgctca
     3901 tccggcctgg aaacagacca acatcatgcc ggtcgtcgaa cgtggcgatc gattcatcgg
     3961 tgttctgcac gcggcgaagc tggccgaaac cctgtcacgt gggcactccg gacacaccga
     4021 cactcccgat gaaacgctct cgggtatgct cgcgcgcggt tactgggata cggtctcggg
     4081 ccttctgcgc gtcggcctgg ccgtcctgcc tccagcgcaa cccgtactgc cgggggacaa
     4141 atgaataccg ccaatctcaa cgcagtcgcc gctctgaatc ggcgttacct gctcgatttc
     4201 ccctatgagg ctgcacgcca actggagtcc atgtcgcccg acgacggtgc cgagctgctg
     4261 gcatcacaac ccctgcacgc ggtagtgcga gcatggcagg tactcggacc cgacgtggcg
     4321 agagccatcc tggagcgcgt accggaggcc atggcacggc atctgttggc cgaggccgaa
     4381 ccgattgcca gcgttgccgt actggccaaa tgggacgaga gcgaacgcga acgccgtctc
     4441 accgagcttg acgcccaggt ggcgcacgag ctgcgcgaac tgctgcgcta tccggaaaat
     4501 tccgcaggac gtttgatgga tcccgatgtc agtccactgc atgccggcct ttccgtgggt
     4561 caggcactgg aacgcctgcg ccggctgaag cgtcacggct tgcgccagtt gtttgtagtg
     4621 gacaacgaag gccacctcac cggccgtgtc gagattcagg atctggcggt ggcagagcat
     4681 gcccggccgc tgcgcgagat cgtactgccg gtgctggcgg tcgtgtacgc gcttgatccg
     4741 caggaggacg tggtgagcat tctcgaacgc cagccgatca ccgaactgcc ggtgatcaat
     4801 gtcgatgggc gctttgtcgg catcattcgc catgcggctc tgctcgccac gctcgaagaa
     4861 gaaacgagca tcgacatcca gaccatggtc ggcgcgagcc gcgacgagcg ggcattgtcc
     4921 agttcgatat ttgctgttcg caagcgcctg ccgtggctgc agatcaatct gctcacggct
     4981 tttctcgccg ccgccgtggt ggggttgttt gaaggcacca ttgccaaatt cacggcgctg
     5041 gcagtactgc tcccggtggt ggcgggccag tcgggtaacg ccggtgctca ggccctggct
     5101 gtgaccatgc gtggtctggt cttgcgtgag atcagcctgc gccactggcc gcgcgtggtc
     5161 gccaaggagg ctggggtggg gctgatcaat ggcctcgccg tggccgcgac cacggcgatt
     5221 ggcgtctacg cctggagcgg ttcgcttggc ctggtgagtg tcatttccag cgccatggtg
     5281 atcgccatgg tcattgccgg tatagccggg gcgctggtgc cgattgcctt gtcccgcttc
     5341 ggcatggacc ccgcccaatc gtcatccatc gtactcacca cagtcaccga cgtggctggt
     5401 ttcttctcct tcctcggaat tgcaaccctg ctggccgaca cgctggcctg agcgaccatc
     5461 aagacggtta acctcttcat gtctgtcgga gctgccctgc aatgccaccc atgattcgcc
     5521 accgattctg cctcctctcc ttcgcgtttt tcgcccagac cttcgcgagt cataccgcct
     5581 tggcagcgga ggaagtatcc gcccagaaat atcctgatgt tctttccgcc acggtgcagg
     5641 cgcgcggagc ggattcattc gactttgacg tcacggtttc ttcaccgtac gactcacccc
     5701 gaagatacgc cgatgcattt cgcgccatgg gccgcgacgg tacggtcttc ggtgagcgta
     5761 cgctcttcca tgatcacgcc ggcgagcagc catttacccg ggatttgtac ggagtgaaga
     5821 tacctcccgg tgtgcgcgca gtcgtgatcc aggccagaga tcagaaatac gggtatggag
     5881 gcaaaaccgt tgaagttgtc ttgcctggtc ggtgaggtgc ggaatcaagg ataatgcgtc
     5941 ttccagcaac atgacgtatc ggagccgcct gcagtgtcga tcgaacacgc caaacaactg
     6001 gccgacaatg gtgccaatcc ccggctgacg ggcgatatgg aattgctcct ggcgatcagc
     6061 caggacttcg ttggcacgcc ggcgatggac gaaacgctgg ctaacgcgat tcagcagatc
     6121 atgagttaca tggacaccga agcggcgtcc attttcctgc tcgacgagat cgatgaaaag
     6181 ctgacctgtc tggcctgtgc cgggccggtc tcgatcaagg ggctgaccat cgacaggaat
     6241 cagggcatcg tcgggcgcgc gctggcggaa aatgccgtcc agatggtgcg cgacgtgtcg
     6301 gccgacggcg acttcagcac cgccgtggac gaccataccg gctttacgac ccgttccatc
     6361 ctgtgcgccc cgctgatggt caagggacgc atgctgggcg cattggaagt gatcaacaag
     6421 cgcaagggtg acggcctttt cgatctggcg gacaagcagt tcctgcaggt gctggccggc
     6481 atggtttcgc tggccgtgca caacgcgcaa atgaccgagg cactggtcga gcagga
//
LOCUS       DAORPM010000129         6506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3126_length_6506_cov_11.390637, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000129 DAORPM010000000
VERSION     DAORPM010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3126_length_6506_cov_11.390637"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(659..1501)
                     /locus_tag="PLD53_07435"
     CDS             complement(659..1501)
                     /locus_tag="PLD53_07435"
                     /inference="COORDINATES: protein motif:HMM:NF004824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRC60783.1"
                     /translation="MTSNNSVWLITGCSTGFGRELARILLQRGHSVVVTARKPSTLDE
                     FSSAENALVAALDVTVPGQIADVVWQAKARFGQIDVLVNNAGYGYLSAIEEGEDDEVR
                     AMFETNVFGLANMTKAVLPGMRERGCGHIVNISSMGGLIGFPGIGYYNATKFAVEGLS
                     EALAKETASLGIKVTIVEPGPFRTDWAGRSLRTPKNAIADYAQSAGARRAAVRGYSGS
                     QQGDPVRGAKAIIQAVESPEPPLHLLLGRPAYEMTVAKFKEFSAEMEKWREVSLGADF
                     PDAG"
     gene            complement(1538..>2061)
                     /locus_tag="PLD53_07440"
     CDS             complement(1538..>2061)
                     /locus_tag="PLD53_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRC60784.1"
                     /translation="SEGGHADFAPRNELETALLLHLRSEHGRVSTERVVSGPGLYNIY
                     RFLRDAGHFPETPAVAEAMRQGDRSAAIAKAALAGECTLCDKALDLFVSLYGAEAGNV
                     ALRVLATGGIYLGGGIAPKIIERLKGPGFMLAFCAKGRLSPLLENVPVRVILNDRTAL
                     FGAARCAMCHKTE"
     assembly_gap    2062..2161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2344..5097)
                     /locus_tag="PLD53_07445"
     CDS             complement(2344..5097)
                     /locus_tag="PLD53_07445"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60785.1"
                     /translation="MKKAEYTRLEEAREGKIGWKKWGPYLSERQWGTVREDYSENGDA
                     WNFFTHDHARSRAYRWGEDGLAGISDDKQRLCFALALWNGKDPILKERLFGLTNSEGN
                     HGEDVKEYYFYLDSTPTHSYMKYLYKYPHAAYPYADLVDTNRSRSRHEMEYELLDTGA
                     FSDDRYFDVFVEYAKDGAEDILAKITVANRGPDAAELHVLPTLWFRNDWAPWIAKSNR
                     ADQKPNLKQIKAAAGTNAVAVSHPSLGEFVLACEGEVPLLFTENDTNSARLFPGQNNA
                     SPYVKDGINNYVVDGSRDAVNPEKQGTKVAAHYRLMVEPGQSTSVRLRLTNQAAVRER
                     GKPKTAATVFGGPFDKIFDARQQEADDFYQAVIPPSVSPDEAGVMRQALAGMLWSKQY
                     FFFDGDNWLDEHNTNPLHSGYQKGRNSEWFHMLNEDIISMPDKWEYPWYAAWDLAFHT
                     LPLAIVDPDFAKEQMKLMLKGTYLHPSGQMPAYEWNFSDVNPPVHAFATLFLLRTEQA
                     QRGAMDVDFLRETFNKLVLNFTWWVNRKDRFGKNVFEGGFLGLDNISVFDRSAPLPTG
                     GHLEQADGTAWMSLFSQNMLELAIALAAHDRTYEDMVFKFAEHFYYIAMAMNKTGQDG
                     MWDEEDGFYYDLLRLPDGSATRLKVRSMVGLLPLCATTVIEKAQRESIPGTLAQVFER
                     LRRMPELKQSIHPTGVGNFGVEERGILSMVNPERLRRILTKMLDENEFLSPYGIRSLS
                     KFHEKHPFVFHVEGQEFRVDYLPGESDSGMFGGNSNWRGPIWVPVNTMIIRALLNFYL
                     YYGDSFKIECPTGSGVMMNLFEVSREIAGRLIRIFTRDENGQRAVYGGEQTFQSDPQW
                     REYILFYEYFHGDNGAGLGASHQTGWTGIVAKLIELFGFMDAEKLLKEGKKSAFALRT
                     KAT"
     gene            complement(5319..>6506)
                     /locus_tag="PLD53_07450"
     CDS             complement(5319..>6506)
                     /locus_tag="PLD53_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60786.1"
                     /translation="LATSGLSLAFGAFEGAWGAFLLFFANFLTILAVAAAIFILAGFV
                     TRAELGTRVDFLKRFSAAGIGLLAVAAILTQQLVATIDDWRLKQSIVTTIEHALADDP
                     GARLANVIYDQVSNGPVNVLATVNTPRSFSPIKVKAMQQSISENVKRDINLFVRCTIT
                     HDVAAAGSANLLPAVNLDGKFVAASVTPNVRMTETAEQTLRELVSHGQTFDLQDVQLV
                     QLPTGPVIVATVSGARPPVPSQVRFAEDTIRKRLGDQTVTLLVSATTTSNVTAKGRVL
                     LGDAQFVPMSAADRTVQQKLEAFGKTRLEQFPSTFVNGLDAAKSGDGWEVRAEVVSAK
                     LLQPADVGMVEKGLAEIAGTSISLSVLTPDGLIVGPKGFTTVQRSVEERFARQLATTT
                     REK"
BASE COUNT         1272 a         1937 c         1830 g         1367 t
ORIGIN      
        1 ttttttcgcc gcttgcccag taatcaggtg cccggcaagc ttatcccaga agtacgagcg
       61 gcttctggtt ttgatgacaa atcattggat taagacgcct catcgaaggt tcactttcgt
      121 tcgtctctct gatccatacc tgcccaaggt atttctcgga ctattgcccc aacgctcacc
      181 accacggctc ttgaccgcag caccttgggg tggtttgagg cctgctcctt aaagccgata
      241 ccgagggacc aacctaccct catcttccgt gcaatttcaa cacaaatctt cagttcgtca
      301 gttcatagcg aacttccttc ctgtgtgcct gcggcacacg cccacattct ccaatgacga
      361 tgatcgaatg gctgcaatga cgagatgagc ggcctctcaa acagcattgc cccaatcatc
      421 gcgtcaccgg aaaaatattc ttcgatcatt ccccctggcc gtccggaatc gctgcattgc
      481 tgccactgct gcggaaatcc tgaatgtctt ttgtgggcac aaatgaggca gttaaaaaac
      541 cataagctca tcatgattcg ccgctaaatg ctgcaacgca gcactggcta cggaatgtga
      601 acacggaata cggatatcca catttagtgt ggccgaagtc tgagcagacc ttcggttatc
      661 agcccgcgtc agggaaatcc gcgcccagag aaacttcgcg ccatttctcc atttccgctg
      721 aaaactcctt gaacttggcg acagtcattt cgtatgccgg ccgacccagg agcaaatgca
      781 gcggtggttc aggcgattcg acggcttgaa tgatggcttt cgcaccacgc accgggtcac
      841 cttgctgact gccgctatag ccccggactg ctgcgcgtcg tgcaccggcg gattgtgcat
      901 agtcggcaat cgcgttcttt ggagtcctga gggagcggcc cgcccagtcc gtgcggaaag
      961 gcccaggctc gacgatcgtg accttgatgc cgagggacgc ggtttccttg gccagggctt
     1021 cggagaggcc ctcgacggcg aacttggtgg cgttgtagta gccgatgccg ggaaagccga
     1081 tcaggccgcc catcgacgaa atattgacga tgtggccgca accccgctct cgcatgccgg
     1141 ggagtacggc cttggtcatg ttggccaggc cgaaaacgtt ggtctcgaac atggcgcgga
     1201 cctcatcgtc ctcaccttcc tcgatggcag aaagatagcc gtagcccgcg ttattcacca
     1261 gcacgtcgat ttgtccgaac cgcgccttgg cctgccagac cacgtcagcg atttgcccgg
     1321 gtacggtgac gtccaatgcc gcgacaagtg cgttttcggc cgagctgaat tcatcgagag
     1381 tcgacggttt gcgtgccgtc accacgacgc tatggccacg ctgaagcagg atgcgcgcaa
     1441 gttcgcggcc gaagccggtc gaacaaccgg taatcagcca aacagagttg ttggaggtca
     1501 tggcatttgc ttcctgttgt tttccgccct atgccgtcta ttctgtcttg tggcacatcg
     1561 cgcagcgcgc tgcgccgaaa agggctgttc ggtcattgag aataacccgc accgggacat
     1621 tctcgagcag cggactgaga cgccccttgg cgcaaaaggc cagcataaag ccgggcccct
     1681 tgaggcgctc gatgattttc ggcgcaatgc cacccccgag atagataccg ccggtggcca
     1741 acaccctgag cgcgacattg ccggcctccg cgccgtacag agaaacaaac agatccaggg
     1801 ctttgtcgca aagcgtgcat tcgccggcca aggctgcttt ggcaatagcg gcagaccggt
     1861 cgccttggcg catggcctcg gctacggcgg gagtttccgg gaaatggccg gcgtcgcgta
     1921 agaagcggta gatgttgtac agcccggggc cggaaaccac ccgctccgtg ctgacccgtc
     1981 catgctcgct gcgcaagtga agcagcaacg ccgtttccaa ttcgttacgg ggagcgaagt
     2041 cggcgtgccc cccttcgctc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 ncgacgattt cctccaagcc ccctgcatcg cgacttgaaa accgatcttc gacaaccggc
     2221 gtcagctttc cgtcagcagt gtcgaaatag gccaatcggg tcgatgttcc accgatgtcg
     2281 cccgcaagaa tcaacatggc tcatccagca tagcgcctgt tgacgtcgcc gcaacccaga
     2341 atttcatgtc gccttcgtgc gcaatgcgaa cgctgacttc ttgccttctt tcaacaactt
     2401 ttcggcgtcc atgaatccga agagctcgat gagcttggcc acgatgcccg tccaaccggt
     2461 ctggtggctg gcgccgagtc cggcaccatt gtcaccgtgg aaatactcgt aaaacaggat
     2521 gtattcacgc cattgaggat cggactggaa tgtctgttca ccgccataga ccgcgcgttg
     2581 gccgttttca tcacgagtga agatccggat gagccgcccc gcaatctccc gactcacctc
     2641 gaagagattc atcatcaccc ccgaacccgt cgggcactcg atcttgaagc tatcgccgta
     2701 gtacaggtag aaattcaaca gggcacgaat gatcatggtg ttcacaggaa cccagatcgg
     2761 ccctcgccag ttggagttac cgccaaacat gccgctgtcc gactcgcccg gcaggtagtc
     2821 cacccggaac tcctggccct cgacgtgaaa gacgaacggg tgcttctcgt gaaatttgga
     2881 cagcgagcgg atgccgtatg gactcaagaa ctcgttctcg tcgagcatct tcgtcagtat
     2941 ccggcgcaaa cgctccggat tgaccatgga caagatcccc cgctcctcta cgccgaaatt
     3001 ccccacgccg gtagggtgga tcgattgctt gagctcgggc atacggcgca ggcgttcaaa
     3061 aacctgggcc agcgtacctg gaatggactc gcgctgtgcc ttttcgatga ccgtggtggc
     3121 gcacaggggc aacaacccaa ccatcgagcg caccttgagt cgcgtcgcgc tgccgtcggg
     3181 caagcgcagc aggtcgtaat agaagccgtc ctcctcgtcc cacatgccgt cctggccggt
     3241 tttgttcatg gccatggcga tgtagtagaa gtgctcggca aatttgaaga ccatgtcctc
     3301 ataggtgcgg tcatgggcgg cgagggcaat cgcgagttcc agcatgttct ggctgaacag
     3361 cgacatccag gcggtaccgt ccgcctgctc aaggtgcccc ccggtaggca gcggtgcgct
     3421 gcggtcgaat acgctgatgt tgtccagccc cagaaaaccg ccctcgaaaa cgttcttgcc
     3481 aaagcgatcc ttgcggttca cccaccaggt gaaattcagt accagtttgt tgaaagtttc
     3541 cctgagaaaa tcgacgtcca tcgcaccgcg ctgcgcctgt tcggtgcgca gcaggaagag
     3601 cgtggcgaag gcatgaaccg gcggattcac gtcgctgaaa ttccactcat aggccggcat
     3661 ctggccgctc gggtgcaggt aggtgccctt gagcatcagc ttcatctgct ccttggcgaa
     3721 gtccgggtcc acgatagcca gcgggagcgt gtgaaaagcc agatcccagg ccgcatacca
     3781 ggggtactcc catttgtcgg gcatcgagat gatgtcttca ttcagcatgt ggaaccactc
     3841 cgaattccgg cccttctgat atccggaatg aagggggttc gtattgtgct catccagcca
     3901 gttgtcgccg tcgaagaaga agtactgctt gctccacagc atcccggcaa gtgcctgacg
     3961 catcactccg gcttcgtccg gcgaaacact cgggggaatc acggcctggt agaagtcgtc
     4021 tgcttcctgc tgccgtgcgt cgaagatctt gtcaaacggt ccgccaaaaa cagtggccgc
     4081 tgtcttgggc ttgccacgct cgcggactgc agcctgattc gtcaggcgca aacgcaccga
     4141 ggtcgactgg ccaggctcca ccatcagccg ataatgcgct gcgactttgg tgccctgttt
     4201 ctccggattc acggcatccc gactgccatc cacgacgtag ttgttgatgc cgtccttgac
     4261 gtacgggctt gcgttgttct ggccgggaaa gagccgcgcg ctgtttgtgt cgttctcggt
     4321 gaacagcagc ggcacctcgc cttcgcaggc gagcacgaat tcgcccagcg acggatgcga
     4381 caccgcgacc gcgttcgttc ccgccgccgc cttgatctgc ttgaggtttg gtttctgatc
     4441 cgcccggttg gatttggcaa tccacggcgc ccagtcgtta cgaaaccaga gcgtcggcaa
     4501 gacgtgcagt tcggcggcgt ccggcccgcg gttggccacc gtaatctttg cgaggatgtc
     4561 ttccgccccg tctttggcat actccacgaa gacgtcgaaa tagcggtcat cactgaaggc
     4621 gccggtatcc agcagttcat actccatctc gtgccggctg cgactgcggt ttgtatccac
     4681 caggtcggcg taggggtagg ccgcgtgcgg atacttgtag agatatttca tgtacgagtg
     4741 ggtcggcgtg ctgtcgaggt agaagtagta ctccttcaca tcctcgccgt ggttgccttc
     4801 gctgttggtc aggccgaaga gtcgttcctt gaggatggga tccttgccgt tccacagcgc
     4861 cagcgcgaag cacagccgct gcttgtcgtc ggaaatgccg gccagaccgt cttcgcccca
     4921 tcggtaggcg cgcgagcggg catggtcgtg ggtgaagaaa ttccaggcgt cgccgttctc
     4981 actgtagtcc tcacgcacgg ttccccactg acgttcgctg aggtaggggc cccatttctt
     5041 ccagccaatc tttccttccc gggcttcttc aagtctggtg tattcggctt ttttcatttc
     5101 ctgttttctc cgttgctgtg gtggcgacta ctcagttcgc tttactgcct tatccccaat
     5161 cgcattgggc cgaaaaaagc tcggttagtt ctcgggcaag aggcaaagac acctcgtcgg
     5221 aaatccggat acctgtcatt ttccctggcc gccgctgtgg caggaatctt ccgggcagac
     5281 gactttcggc caccccgtgg cggcttcgac caatcgattc atttttcccg agtggtggtg
     5341 gcaagctgcc gggcaaacct ctcctcgacc gagcgttgca ctgtggtgaa acccttcggc
     5401 ccgacgatca agccgtctgg cgtgagtacg gaaagggaga tgcttgtacc ggcaatctcg
     5461 gcaaggccct tttccaccat tcccacatcg gctggctgaa gcagcttggc actgacaact
     5521 tccgcccgca cttcccaacc gtctccagac ttcgccgcat caaggccgtt gacgaaggta
     5581 ctcggaaact gctccagcct ggttttcccg aacgcttcaa gtttctgttg aacggtccgg
     5641 tcggccgcgg acatcggtac gaactgtgcg tctccaagca gcacgcgccc cttggcggta
     5701 acgttgctgg tggtcgtggc gctcaccagc agggtgaccg tctgatcgcc gagccgcttg
     5761 cggatcgtgt cttcggcaaa ccggacctgc gagggaacag gcgggcgcgc accggacacc
     5821 gtagcaacga tcaccggacc cgtcggcaat tggacgagtt gcacgtcctg caagtcgaaa
     5881 gtttgaccat ggctcaccaa ttcccggaga gtctgctcgg cggtttccgt catccgtaca
     5941 ttgggcgtca cgctggcggc aacgaacttg ccgtcgaggt tcactgccgg caggagattc
     6001 gccgaaccgg ccgctgccac gtcgtgagtg atagtgcacc tgacgaacag gttgatatcc
     6061 cgcttgacgt tttccgagat cgactgctgc atggccttga ccttgatcgg ggagaatgat
     6121 cgcggtgtat tgactgtcgc cagcacattg accgggccgt tcgacacctg atcatagatg
     6181 acattcgcaa gtcgggcacc gggatcgtct gccagggcat gctctatggt ggtgacgatc
     6241 gactgcttga ggcgccagtc gtcaatggtg gcaacgagct gctgcgtaag gatggcggcg
     6301 acggcgagca ggccgatacc ggccgcggag aagcgcttca ggaagtcgac gcgcgtgcca
     6361 agttcggcac gggtgacgaa tccagccaga atgaagatgg cagccgcgac agccaatatc
     6421 gtcaggaagt tggcgaagaa cagcaggaag gctccccagg cgccttcgaa agctccgaat
     6481 gccaacgaga gtccggaggt ggcgag
//
LOCUS       DAORPM010000130         6496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3137_length_6496_cov_17.801894, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000130 DAORPM010000000
VERSION     DAORPM010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3137_length_6496_cov_17.801894"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            576..1763
                     /locus_tag="PLD53_07455"
     CDS             576..1763
                     /locus_tag="PLD53_07455"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC60787.1"
                     /translation="MSGIRVIDVGTFLAGPYAASMLGEFGAEILKVEHPINGDPMRRF
                     GTASKRHDATLAWLSEGRNKKSVTMDLRQREGVELFLKLVAKSDVLIENFRPGTMEEW
                     GLGWDVLSKANPALVMLRVSGYGQTGPYRSRSGFAHIAHGFAGLSYLAGFPGETPVVP
                     GTTPLGDYLSSLYGAIGILLALRHSEATGEGQIIDIGIYEAVFRQMDEIAAAYGMFGK
                     VRQREGAGSFVAVPHGHFRTIDDKWVAIACTTDKMFERIAEAMGRPELASAEYYGNQR
                     KRLAARDEVNRIVIEWVGSLTRDEIMKLCIDGEVPIGKLNSIADIFEDEHFLARGNLA
                     HIKEDGVGDVVIPGVVPTLSETPGRISNLGPPLGNATYEVLRELLGITGPEIQRLRQR
                     KII"
     gene            1788..3014
                     /locus_tag="PLD53_07460"
     CDS             1788..3014
                     /locus_tag="PLD53_07460"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRC60788.1"
                     /translation="MNTEQTETRLPLEGIRVIDAATVIAAPYSACILGEFGAEVIKVE
                     NPKGGDPMRYFGTPTKRKDTLAWLSEARNKKSVTLDLHLPEGVELFKKLIENSDVLCE
                     NFRPGTLEKWGLGWEVLHKINPRLIMLRVTGYGQTGPYKDRPGFARVAHAVGGIAFLS
                     GMPRGTPVTPGSTTIGDYLTGLYGCIGILMALRHRERTGCGQYIDAALYESVFRCTDE
                     LAPAYGMYGTVRERHGPTHNDFACPHGHFPTKDNGKWVAISCVTDKLFERLAAAMGRP
                     ELAAPHVFGDQKVRMEHRHDVNEIVRDWCGSLHREELLARCFAAGAPAGPLNSIADVF
                     GDRQFHSRRNLVAIDEPDIGETVIVPSVMPRLSVTPGRIKHLGPKLGQNTDEVLSGLL
                     GLEPQEIEELRKKRVI"
     gene            3056..4276
                     /locus_tag="PLD53_07465"
     CDS             3056..4276
                     /locus_tag="PLD53_07465"
                     /EC_number="5.4.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylfumaryl-CoA isomerase"
                     /protein_id="HRC60789.1"
                     /translation="MDGILKGLRVVEGTAFVAAPLGGMTLAQLGADVIRFDPLDGGLD
                     NSRWPLTLDGKHSLFWAGLNKGKRSIAVDFRKPEGQEILTRLICAPGENAGLFSTNFP
                     AKGWLSYETLKAHRQDLIMINFLGRRDGGSEVDYTVNPQVGLPFLNGPINSSEVVNDA
                     FPAWDYTSGHMIAIGLLAAERHRRLTGEGQLVRLSLKDVALAMLGNLGLLAEVMVNNA
                     DRPKYGNYLYGSFGRDFRTLDGKRLMIVGLTDMQWRCLTKATGLQDAISELGVRLGLD
                     LTDEGNRFRARQEIAALMEPWFQARTMAEAARILDANRVTWGPYRGVREAIATDPDCS
                     LDNPMFSTVDQPGIGSYLAPATPLDFNYVPRLPAKLAPVLGEHTDQILLDILGMSEGE
                     VGRLHDQGIVAGPG"
     gene            4342..5385
                     /locus_tag="PLD53_07470"
     CDS             4342..5385
                     /locus_tag="PLD53_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRC60790.1"
                     /translation="MKLPVHFYKPLAIGAPQPLRELPVRPERMIHFFPPHIEKIRSKA
                     PETAKQCDVMCGNLEDAIPIESKDAARAGFIELLSNNDFGDTGMWVRINALNSPWVLD
                     DLSEIIKHVGNKVDVIMVPKVEGPWDIHFVDQYVSLLEAKYAIKKPILLHALLETAQG
                     VTNVEAICGASPRMHGLSLGPADLAASRGMKTTRVGGGHPGYGVLVDEEEGKSQRAFY
                     QQDLWHYTIARMVDAAVAHGLHSFYGPFGDLKDEAACESQFRNAFLMGCSGAWSLAPN
                     QIAIAKRVFSPDVKEVLFAKRILEAMPDGSGVATIDGKMQDDATWKQAKVIVDLAQLV
                     AKKDPELAAAYGW"
     gene            5424..6476
                     /locus_tag="PLD53_07475"
     CDS             5424..6476
                     /locus_tag="PLD53_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRC60791.1"
                     /translation="MKNRLGNYFEDFSIGQKIVHATPRTVTEGDVALYTALTGSRFAV
                     GSADAFALGLGFPRSPVDSMLAFNIVFGKTVPDISLNAIANLGYASGRFGQPVYPGDT
                     LSSTSTVIGLKENRDGKTGVVYVNSRGTNQRGEMVLDYCRWVMVRKKDAASPAPAAVV
                     PELPDSVAAADLVVPVDLEIEKYDCALAGSTDLWNDYQVGEQIDHVDGMTIEESEHMM
                     ATRLYQNTAKVHFNQHAEKSGRFGRRIVYGGYVISMARSLSFNGLANAFSIAAINAGR
                     HVSPAFAGDTIYAWSSVVDKMALPGRNDLGALRLRTVATKDRPCADFPYKLDDGSYDP
                     SVLLDFDYTVLMPSRA"
BASE COUNT         1351 a         1811 c         1951 g         1383 t
ORIGIN      
        1 gggaagaaag tagaaccagt gccacagcgg gggcaatgcg ctcccattct cgaatcgagt
       61 aagcctatcg tcgagcgttg ccgccgcagc cagtgtaggt gatacgctca agtcgtcatc
      121 cgtagcctct ttgcggccaa tccattcagc gaaatctgtt gtcgattcgg tcatcaacct
      181 gttcctttcg ggttttccgg ataaggttgg caagttaaac acagccggtt ttctaactca
      241 agttgcttga tgaattttgc tcggacggga cgaaacagta ggataatccg ccgttgggat
      301 agccgtttgc tcatttgcta ttgaatttat gttttgctat atttcgcaac cccgcttggc
      361 gggaattggc ggtattgaag cgtttcaagg cattgcagtc gagaggaact actatgaaaa
      421 agggagctgg aagcgcgaag tctgagtctg ctccgctgca gtctgaatct gcgaccgctg
      481 tggacgaatc atccgtcagt gtctcaggca cgaacactga tgcttctcgg gcgggctctg
      541 accaggatcg ctcgcccgat acagagaatc tgccgatgag cggtattcgc gtcatcgacg
      601 tcggaacctt cctggcagga ccgtatgcag cgtcgatgct aggcgagttt ggtgccgaaa
      661 tactcaaggt cgagcaccca atcaacggcg acccgatgcg aagattcggc accgcgtcga
      721 agcgccacga tgcgacattg gcctggctca gcgaaggccg caacaagaaa tcggtcacca
      781 tggatctgcg ccagcgggaa ggcgtcgaac tgtttctgaa actggtcgcc aagtccgatg
      841 tgctgatcga gaatttccgg ccgggaacga tggaggaatg gggtctcggc tgggatgtgc
      901 tgagcaaggc caatccggcg ctggtgatgc tccgtgtttc ggggtacggg cagaccggac
      961 cttatcgctc gcgctcggga ttcgcgcaca tagcccatgg cttcgccggt ttgtcctacc
     1021 ttgccggctt cccgggtgaa accccggtgg ttcccggcac caccccgctg ggtgactacc
     1081 tgtccagcct ctatggtgcc attggtatac tcttggcatt gcgtcacagt gaagcgaccg
     1141 gcgaagggca gatcattgat atcggtattt acgaggccgt tttccgccag atggacgaga
     1201 tcgccgcagc ctatggcatg tttggcaagg ttcgccaacg cgaaggcgcg ggcagtttcg
     1261 ttgccgtccc gcacggacat tttcgcacca tagatgacaa atgggttgcc attgcttgca
     1321 ccacggacaa gatgttcgaa cgcattgcgg aggcgatggg caggccggaa cttgcttcgg
     1381 ccgagtatta tggaaatcag cgcaaacgct tggcggcacg cgatgaagtt aacaggatcg
     1441 tcattgagtg ggtcggttcg ctgactcggg acgagatcat gaagctctgt atcgatggtg
     1501 aagtgccgat cggcaaactc aacagcattg ccgacatctt tgaagacgaa cacttcctgg
     1561 cgcgcggcaa tctggcgcat atcaaagaag atggcgtggg tgatgtggtg attccgggag
     1621 tcgtgccgac actgtctgaa acgcccggcc ggatttccaa tctcgggcca ccactgggta
     1681 acgcgaccta cgaagttctg cgcgagttgc tcggaattac aggaccggaa attcagcgct
     1741 tgcggcaacg caagatcatc tgatcatttc atcgcacaag gattcccatg aacacagagc
     1801 agaccgaaac caggctaccg ctcgaaggca ttcgtgttat cgatgcggcc acggtcattg
     1861 ctgcgcctta cagcgcctgc atcctcggtg aatttggcgc cgaggtcatc aaggttgaaa
     1921 accccaaggg cggtgatccc atgcgttact tcggtacgcc gaccaagcgc aaggatacgc
     1981 tggcctggct gagcgaagca cgtaacaaga aatcggtgac gttggacctg catttgcccg
     2041 aaggcgtcga gttattcaag aagctgatcg agaactcgga tgtgctgtgc gaaaacttca
     2101 ggcccggtac gctcgaaaaa tgggggcttg gctgggaggt gctgcacaag atcaatcccc
     2161 gcctgatcat gctgcgtgtt accggctatg gtcaaaccgg cccttataag gaccggcctg
     2221 gttttgcccg cgttgcgcac gctgtcggtg gaatcgcttt tctctcgggc atgccgaggg
     2281 gaacgccggt tacaccgggg tcgaccacca tcggtgacta cctgaccgga ctctatggct
     2341 gcatcggcat attgatggcc ttgcgccatc gtgaacgtac cggctgcgga cagtacatcg
     2401 acgccgcact ctacgaatcg gtgtttcgtt gcaccgacga gttggcgccg gcttatggca
     2461 tgtacggaac ggtgcgtgag cgacatgggc cgacgcacaa cgattttgca tgtccgcatg
     2521 gccacttccc gaccaaggac aacggcaagt gggtggcaat ttcgtgtgtg actgacaaac
     2581 tgttcgagcg gctggcagcc gccatgggtc gccccgaact ggccgccccc cacgttttcg
     2641 gggaccagaa ggtgcgcatg gagcaccgtc acgacgtcaa cgagattgtc cgcgactggt
     2701 gcggttcgtt acatcgcgaa gagctgctgg cgcgctgctt tgccgctggt gcgccagctg
     2761 gtccgctgaa cagcatcgcc gatgttttcg gtgaccgtca gtttcactcg cgacgcaatc
     2821 tggtggccat cgacgaaccg gacatcggcg agacggtcat tgtgccatcg gtgatgccac
     2881 gcctttcggt aacgccaggg cgcatcaagc atctcggtcc gaaacttggc cagaatactg
     2941 atgaagtcct gtccggactt ctcggactgg aaccgcaaga gatcgaagag ctgcgcaaaa
     3001 agcgtgtgat ctgaaaccac tctagctgag caccacttcg gaataggtag acaggatgga
     3061 tggaattctc aaaggtttgc gtgtcgtcga gggcacggct tttgtcgccg cgccactggg
     3121 cggcatgact ctggcgcaac tgggcgccga tgtcatacgt ttcgacccgc ttgacggcgg
     3181 gcttgacaac agccgctggc cgctgacgct ggatggcaaa cacagcctgt tctgggccgg
     3241 cctgaacaag ggcaagcgct cgatcgctgt cgattttcgc aagccggagg gacaggagat
     3301 attgactcgg ttgatctgcg cgccgggtga aaatgccggc ctgttcagca ccaattttcc
     3361 ggccaagggc tggctcagct atgaaacgct gaaagcccat cgtcaggatc tgatcatgat
     3421 caactttctc ggtcgtcgtg atggtggttc ggaggtcgat tacacggtaa atcctcaggt
     3481 cgggctgcct ttcttgaatg gcccgatcaa ttcgtcggag gttgtgaacg acgcgtttcc
     3541 ggcgtgggat tacaccagcg ggcacatgat cgccattggc ctgctcgctg ccgaaaggca
     3601 tcgacgcctg accggcgaag ggcaacttgt gcgcctgtcg ctgaaggatg tggcgctggc
     3661 gatgctcggc aaccttggct tgctcgccga agtgatggtc aataacgcgg accggcccaa
     3721 gtacggcaac tacctctatg ggtctttcgg acgtgatttc agaacgctcg acggtaaacg
     3781 tctgatgatc gttggcctga ccgacatgca gtggcgttgc ctgaccaagg ctaccggcct
     3841 ccaggatgca attagtgaac tcggtgtccg gcttggcctt gatctgactg acgagggcaa
     3901 tcgatttcgt gctcgccagg agatcgcggc gctcatggag ccctggttcc aggcccgcac
     3961 aatggctgaa gcagcccgga ttctcgatgc caatcgagtc acctggggtc cttaccgtgg
     4021 cgttagggaa gccatcgcca ccgatccgga ttgctcgctc gacaatccga tgttctcgac
     4081 cgtcgaccag ccgggtatcg gttcctatct ggcgccagcc acaccgcttg atttcaatta
     4141 cgtaccccgt ttgccggcaa agctggcgcc agtactcggg gagcataccg accagattct
     4201 gctcgacata ctcggtatga gcgagggcga agtcggccgt ctgcacgatc agggtattgt
     4261 cgccgggcca ggttgaaaca ctgcgtgtag catgatcgct ccaaccaatc caccaccgct
     4321 gtctagatta aggaaactgc catgaagcta cccgtccatt tctacaagcc gcttgccatt
     4381 ggcgcgccac agcccttgcg tgaacttccg gtgagaccgg aacgaatgat tcatttcttt
     4441 ccgccgcaca tcgaaaaaat ccgctccaag gcgcccgaga ccgccaaaca atgcgatgtc
     4501 atgtgcggca atcttgagga cgccatcccg atcgaatcca aggacgctgc gcgcgccggc
     4561 ttcatcgaat tgctgagcaa caacgatttc ggcgacaccg ggatgtgggt gcgcatcaat
     4621 gccttgaaca gcccctgggt tctcgacgac ttgagcgaga tcatcaagca cgtcggaaac
     4681 aaggtcgatg tcatcatggt tcccaaggtc gaggggccgt gggatattca tttcgttgac
     4741 cagtatgtct cgctactcga agccaagtac gcgatcaaga aacccattct gttgcacgct
     4801 cttctcgaaa cggcacaggg tgtgaccaat gtcgaagcca tctgcggcgc aagtccgcgc
     4861 atgcatggct tgagcctcgg cccggccgat ctggccgctt cgcgcggcat gaagacaacc
     4921 cgcgtgggtg gcggacatcc cggctacggt gttctggttg acgaggaaga aggcaagagc
     4981 cagcgggcgt tctatcaaca ggatctctgg cattacacca ttgcgcgcat ggttgacgcc
     5041 gccgtggcgc acggtctgca ttcgttctac ggaccgtttg gcgatctcaa ggacgaagcc
     5101 gcctgcgaat cgcagttccg caatgccttc ctgatgggct gctcgggcgc ttggtcgctt
     5161 gcgccgaatc agatcgccat cgccaagcgg gtattcagcc ccgacgtcaa ggaagttctg
     5221 tttgccaaac gcatcctgga ggcgatgccg gatggcagcg gcgttgcgac aattgacggc
     5281 aagatgcaag atgacgctac ttggaagcag gccaaggtta tcgtcgatct ggcgcaactg
     5341 gtcgccaaaa aggatccgga gctggccgca gcatacggct ggtagggctt aactcagcac
     5401 acgggagtga aaatcgaatg agcgtgaaga acaggttggg caactacttt gaggacttca
     5461 gtatcgggca gaagattgtc catgcgacgc cgagaacggt gaccgaaggc gatgtggcgc
     5521 tgtacaccgc attgaccggt tcccgtttcg ccgtcggttc ggcagacgcc tttgctctcg
     5581 gtctgggctt tccgcgatcg ccggtggact ccatgctggc tttcaacatc gtgttcggca
     5641 agaccgtgcc cgacatttcc ctgaatgcca ttgccaacct cggctacgcg tcggggcgct
     5701 tcggacaacc ggtctatccg ggggatacgc tgtcctccac ctcgacggta attggcctga
     5761 aggagaatcg cgacggcaag acaggcgtcg tgtatgtgaa ctcgcgcggg accaatcagc
     5821 gcggtgagat ggttctcgac tactgtcgct gggtcatggt acgcaagaag gatgccgcct
     5881 cccccgcccc agcggcggtg gtgccggagc ttcccgattc tgtcgcggcg gcggatctgg
     5941 ttgtcccggt ggatttagaa attgaaaagt atgactgcgc gttggccggc agcaccgacc
     6001 tgtggaacga ttatcaggtc ggcgaacaga tcgatcacgt agatggcatg accatcgagg
     6061 aaagcgaaca catgatggct acccggctgt accagaacac ggccaaggtg cacttcaacc
     6121 agcatgccga gaaatcggga cgatttggcc gccgtatcgt gtacggtggt tacgtcatca
     6181 gtatggcgcg ttcgctgtcc ttcaatggac tggcgaatgc cttcagcatc gcggcgatca
     6241 atgctggtcg gcacgtatcc ccggcgtttg ccggcgatac gatttatgcc tggtcgtcgg
     6301 tggtcgacaa gatggcgttg ccaggccgaa acgatctcgg cgcgcttcgc ttgcgcaccg
     6361 tggcgaccaa ggaccgtcct tgcgctgatt tcccgtacaa actcgacgac ggcagctacg
     6421 acccgagcgt cctcctcgac tttgactaca cggtattgat gccgagcaga gcgtagccgt
     6481 ttttttggat gcttag
//
LOCUS       DAORPM010000131         6442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3175_length_6442_cov_7.593863, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000131 DAORPM010000000
VERSION     DAORPM010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3175_length_6442_cov_7.593863"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLD53_07480"
     CDS             complement(<1..515)
                     /locus_tag="PLD53_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRC60792.1"
                     /translation="MEYLAAFIDIVLHLDKHLAVLVQQYGPWIYAILFTIIFSETGFV
                     VTPFLPGDSLLFVAGALAALGGMDIGVLLAVLIAAAVLGNMLNYQIGRFLGPKVFHWE
                     NSRFFNKAALVKTHAFYEKHGGKTLVISRFLPLFRTFAPFVAGIGAMSYSRFTFFNLA
                     GAISWVASLSLA"
     gene            578..2425
                     /gene="mutL"
                     /locus_tag="PLD53_07485"
     CDS             578..2425
                     /gene="mutL"
                     /locus_tag="PLD53_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRC60793.1"
                     /translation="MSDTPTRRSGIHILPDLLISQIAAGEVVERPASVLKELLENALD
                     AGSSSVQIQLEEGGVKLIRISDDGCGIARDELALALTRHATSKIASLNDLERVATLGF
                     RGEALASVAAVARVTLTSRQAGDESASAHAWRLTAEPQAQPAPAALHRGTVVEMRDLY
                     YNTPARRKFLKSEATEFAHCAETVKRIALSHPDVAFTLSHNGRVSLHLASNDLRGRAG
                     AILGNEFLTESRAVDTGKISDAVGDSAGNGHRLRVYGYCALPAHSRARSDAQYCYVNG
                     RFVRDKMLSHALRAAYQDMLHGSRFPAYCIFVDLDPASVDVNVHPAKTEVRFRDARAV
                     HQFVFHAVQRTLSSSLAGMRDALPARNEDEARPAFNHWAPRQESLRISEPAVASYLAF
                     AQLANVATTAQSVQHTTYTALSDALSLSEAPPLGYALAQLHGIYILAQNAQGLIVVDM
                     HAAHERILYEKLKNALDGHQVATQALLIPAVFNADALDVAAAEEQTEALHALGFDLAP
                     MGPTQLAVRAVPALLQAADPATLTRALLGELREHGISQLMAARRNELLATMACHGAVR
                     ARRLLSIQEMNALLRQMEETERVDQCNHGRPTWTQVSIDELDRLFQRGR"
     gene            2422..3369
                     /gene="miaA"
                     /locus_tag="PLD53_07490"
     CDS             2422..3369
                     /gene="miaA"
                     /locus_tag="PLD53_07490"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRC60794.1"
                     /translation="MSAATPYPPAILLMGPTASGKTAVAMALAQRFPVELISVDSAQV
                     FRDMNIGTAKPDADTLTTFPHHLIDLISPEESYSAARFCVDALAVMADVTARGNIPLL
                     VGGTMLYFKALLEGLADLPQACPETRADIDAQAIAQGWPAMHAELATLDPETAKRLHP
                     GDSQRIQRALEIVRLTGRSMTALIADGKKKVPPYAFLSLGLLPSQRGVLHERIAERFD
                     AMLKAGLEEEVRMLRSKYRLNLNLPSMRCVGYRQVWEVQDGLAPRPEMRDRGIYATRQ
                     LAKRQITWLTNTLRPERFDCLAENLESQILGRVASALGG"
     gene            complement(3390..6077)
                     /locus_tag="PLD53_07495"
     CDS             complement(3390..6077)
                     /locus_tag="PLD53_07495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HRC60795.1"
                     /translation="MSQLQLRKLFSPSSIAVFGVTEEEGSIGYALLQNLLVSYKGAVF
                     PIHPELESILGRTCYASLADVKVEMGDTVDLAAIIAPAAEVPLLIEACGLAGVRAIVL
                     FSEGFRDAGSSGARIERAMLDVARRYRVRIIGPRSFGLFCPRLGLNLTPIAKQAPVGN
                     IALISQSSTICASILDWSYNNEFGFSSIVSLGNAADLDFAEIIDYLAMDINTHCILVY
                     MENLRDSRRFMSAVRAAARIKPVIAVKAGKAQTSETIALAQTGPVVGDDKVYDAALRR
                     AGVVRVQTIGDMFSAARALTSPKRPAGNRLAVITNGGGPSVMAADLAVSLGIEIPALS
                     AQTISSLNACLPRSWSHCNPVDVLFDATADRYRSALSICLNDENVDAVLVIFAPSTMV
                     EPESVAQAVLEAAADNRKPVLTCWMGEMQVRGARGAFAAARIPTFRSPENAIEAFEFM
                     VSYFRSQNILLEAPASFSNEDEPDIENARLIIEAALADRRRVLSTLEAKAVLAAFRLP
                     VSHSVMARSPNEAMLIAQQLGFPVAMKVESPEIAHKTDIGGVRLNLSNAQAVRSAYNE
                     IVANVAELAPGTRIDGILIEPYISKPHGRELAVGLTCDPLFGPVITFGEGGQVAEVFN
                     ERSVALAPLNQRLIDDLVGRARVAPLLEPFRRMPAVDRSALDSVLQRVSEMACELPWI
                     RKMEINPLIIDDQGAIAVDARISIITHVTSSEPYGHMAICPYPAYLESSWQFTGGTNF
                     TIRPVRPEDAEMIQKFVQSLSGDARYKRFMHAMNELSLSALARLTQIDYDRALVLIAV
                     SHSYGRDAVVGIANYGVLPDGETCDFGLVTSEDVRNKGLGARLMNSLMEVAHNRGIKT
                     MEGEVLVNNPTMLKLVRALGFEIFEHEEDEDLQRAIRQL"
BASE COUNT         1348 a         1996 c         1888 g         1210 t
ORIGIN      
        1 gccaaggaca gcgatgccac ccatgaaatc gcgccggcca ggttgaagaa ggtgaaacgc
       61 gaatagctca ttgcgccgat tccggcaaca aaaggtgcaa aggtgcggaa cagcggcagg
      121 aagcgcgaaa tcaccagcgt cttgccgcca tgcttttcgt agaaggcgtg cgtcttcacc
      181 agtgccgcct tgttaaaaaa ccgcgagttt tcccagtgaa aaacctttgg cccgagaaaa
      241 cggccaatct ggtagttgag catgttgccc agtaccgccg ccgcgatgag tacggcaagc
      301 aacacgccaa tgtccatgcc acccagtgcg gccagcgcac cggcgacgaa aagcagcgaa
      361 tcaccgggta gaaagggcgt caccacaaag cccgtttcgc tgaagatgat ggtgaagaga
      421 atggcgtaga tccatggccc gtactgctgc accagcacgg cgagatgctt gtccaggtgc
      481 aggacgatat cgataaaggc ggcgaggtat tccatcgtgg tgattgcccg gaacaaaggc
      541 gcgattctac ccgaagcccg gccggtataa tctgtccatg tccgacaccc ccactcgccg
      601 ttccggaata cacatcctgc ccgatttgct gatcagccag atcgccgcgg gcgaagtggt
      661 cgagcgtccg gcttcggtgc tcaaggaatt gctcgaaaac gctctggatg cgggcagctc
      721 aagcgtccag atccagctcg aggaaggcgg tgtcaaactg atccgcatca gcgacgatgg
      781 ctgcggcatc gcccgggatg agctggcact cgcgctcacc cgtcatgcca catcgaagat
      841 cgcctcactg aacgatctgg agcgtgtcgc cacgctgggc tttcgcggcg aagcgctggc
      901 ctcggtcgcc gccgtggcgc gcgtcacatt gaccagccgg caagcgggcg acgagtcggc
      961 ctcagcccac gcctggcgac tcaccgccga acctcaagcg caacccgcgc cggcggcatt
     1021 gcaccgcggc acggtcgtcg agatgcgcga tctgtactac aacacgccgg cgcgtcgcaa
     1081 gtttctcaaa tccgaagcga cagagttcgc gcattgcgcc gaaaccgtca agcgcatcgc
     1141 gctttcgcac cccgacgtgg cgttcacgct gtcgcacaat ggccgcgtca gcctccatct
     1201 ggccagcaat gatttgcgcg gacgcgctgg ggccatcctg ggcaacgaat tcctgaccga
     1261 atcgcgcgct gtcgacaccg ggaagatcag cgacgccgtt ggcgatagcg cgggtaacgg
     1321 tcaccgcttg cgcgtttatg gttactgcgc cctgcccgcc cattcgcgtg cgcgcagcga
     1381 tgcccagtac tgttacgtca atgggcggtt tgtgcgcgac aagatgctca gccacgcgct
     1441 gcgtgcggcc taccaggaca tgctgcacgg aagccgcttc ccggcgtact gcatctttgt
     1501 cgacctcgat ccggcgagtg ttgacgttaa cgtccatccg gccaagacgg aagtccgctt
     1561 ccgcgacgct cgcgcggtgc atcagtttgt ctttcatgcg gtacagcgaa cgctgtccag
     1621 ctcgctagcc gggatgcggg atgcattacc ggcgcgcaat gaagatgagg cccgcccggc
     1681 attcaaccac tgggcaccgc gtcaggagtc tctgcgtatc agcgaaccgg ctgtcgcgtc
     1741 gtatctggct ttcgcccaat tggcgaatgt tgcgacgacg gcgcaatcgg tgcaacacac
     1801 gacctacacg gcgctgtccg atgcactctc gctcagtgaa gccccgccat tgggctatgc
     1861 gctggcgcaa ctgcatggca tctacattct cgcccagaat gcgcaggggc tgatcgtcgt
     1921 cgacatgcac gcggcgcatg aacgaatcct ttacgagaag ctcaagaatg cgcttgacgg
     1981 acatcaggtg gcgacgcagg cgctgctcat ccctgccgta ttcaacgccg acgcgctcga
     2041 cgtcgccgcc gccgaagagc agaccgaagc gttgcacgcg ctcggttttg acctcgcacc
     2101 gatgggtccg acgcaacttg ccgtgcgcgc cgtgcccgcg ctattgcagg ctgcggatcc
     2161 ggcgacactg acccgcgcgc tgctcggcga acttcgcgag catggcatct cgcaactgat
     2221 ggccgcgcga cgtaacgaac tgctcgccac catggcctgc cacggtgcgg tacgcgcgag
     2281 acggctgttg tcgattcagg aaatgaacgc cctgctgcgc cagatggagg aaaccgaacg
     2341 cgttgaccag tgcaaccatg gtcgaccaac ctggacgcaa gtgtcgatcg acgaactcga
     2401 ccgcctgttt cagcgcggcc gatgagtgcc gcaacgccct atccgccggc cattctcctg
     2461 atgggtccga ctgccagcgg gaaaaccgcc gtggcgatgg cactggcgca acgctttccc
     2521 gtcgaattga tcagcgtcga ttcggcgcag gtgtttcgcg acatgaatat tggcaccgcc
     2581 aagccggatg ccgacacctt gaccacattt ccccaccacc tgattgacct gatcagcccc
     2641 gaggaatcct actcggcggc ccgtttctgc gtcgatgcat tggcggtgat ggccgatgtc
     2701 acggcacgcg ggaacattcc actgctcgtt ggcggcacga tgctctactt caaagctctg
     2761 ctcgaaggcc tcgccgacct gccgcaagcc tgcccggaaa cacgcgccga cattgacgca
     2821 caggccatcg cacagggttg gccggcgatg cacgccgaac tggccacgct cgacccggaa
     2881 accgccaagc gccttcatcc tggcgattcc cagcgcattc agcgcgcgct ggagatcgtc
     2941 cgcctgacgg ggcgttcgat gacggcactg atcgccgacg ggaagaagaa ggtaccgccc
     3001 tacgcgtttc tatcgcttgg gttgctgcct tcgcagcggg gcgtgctgca cgaacgcatc
     3061 gctgagcgct tcgacgccat gctcaaggcc gggctcgaag aagaagtccg aatgcttcgg
     3121 tcgaaatacc gattgaacct caacctgccg tcgatgcgat gcgtcggcta ccggcaagta
     3181 tgggaggtgc aggatggact ggcgccgcgc ccggaaatgc gcgaccgagg catctacgcc
     3241 acgcgccaat tggccaaacg gcagatcacc tggctaacca acaccctgcg cccggaacgc
     3301 ttcgactgcc tggcggaaaa tcttgaatcg cagatccttg gacgggttgc cagcgccctg
     3361 ggcggctgag ccacactcgg gcacggcccc tacaactgcc tgatggcgcg ctgcaaatct
     3421 tcgtcttcct cgtgctcgaa gatctcgaaa ccaagcgccc gcaccagttt aagcattgtc
     3481 ggattgttga ccagcacttc gccttccatt gtcttgatgc cgcgattgtg agccacttcc
     3541 atcaggctgt tcatcaggcg cgcgccgagc cctttattgc ggacatcttc cgaggttacc
     3601 aagccaaaat cacaggtttc accatcgggt agcacgccat agttggcgat gccgacaacg
     3661 gcgtcccgac cgtaactgtg actgacggcg ataaggacga gcgctcggtc gtagtcgatt
     3721 tgagtaagac gcgccagtgc cgatagcgac agctcgttca tcgcgtgcat gaatcgtttg
     3781 taacgcgcgt cacccgacag ggattgcacg aacttttgta tcatttcggc gtcttcggga
     3841 cgaacggggc gaatggtgaa atttgtaccg ccggtaaact gccacgacga ctcaagatag
     3901 gccggatagg gacagatggc catgtggcca tacggttcgg acgaggtcac atgggtgatg
     3961 atgctgattc gcgcatcgac ggcgatggcc ccctgatcat caatgatcag gggattgatc
     4021 tccattttgc gtatccacgg caactcgcaa gccatttcgg agacacgctg cagcacgctg
     4081 tcaagcgctg accggtcaac cgccggcatt cgccggaacg gttcgagcag gggcgcgacg
     4141 cgtgcgcgac cgaccagatc gtcaatcagg cgctggttga gcggcgccag agcgaccgac
     4201 ctttcattga agacttcggc cacctgcccg ccctcgccga aggtgatgac cgggccgaac
     4261 agcggatcgc aggtcaagcc taccgccagt tcgcgcccgt ggggtttcga tatgtacggt
     4321 tcgatcagga tgccgtcgat acgcgttccg ggcgccagct cggcaacatt ggcaacgatc
     4381 tcgttgtaag cgctacgtac cgcttgcgcg ttcgaaaggt tgagccttac gccgccaata
     4441 tccgttttgt gggcaatttc cggtgactca accttcatcg caaccgggaa cccaagctgc
     4501 tgggcaatca gcatggcctc attcggcgaa cgtgccatca cggagtgcga tacgggcagt
     4561 cggaaagcgg caagcacagc cttggcttca agcgtggata gcactctgcg tcgatcggcc
     4621 agtgcggcct cgatgatcag tcgcgcgttc tcgatgtccg gctcgtcttc gttcgagaac
     4681 gacgcgggag cttcaagcag gatattctgg cttcggaaat acgacaccat gaactcgaag
     4741 gcttcaatgg cattctcggg tgagcggaag gttggaatcc gagccgccgc gaatgcacca
     4801 cgcgcaccgc gtacctgcat ctcacccatc cagcaggtca gtaccggttt tcgattgtcg
     4861 gcggcggctt cgagaaccgc ctgcgcaacc gactccggct caaccatcgt gctgggggca
     4921 aaaatcacca gaacggcatc gacgttttca tcgttcaggc agatcgatag tgcgctgcgg
     4981 taacgatctg ccgtggcgtc gaaaaggacg tcgaccgggt tgcagtgcga ccagctccgc
     5041 ggcaaacagg cattcaggct gctgatggtc tgtgcagaca gggcgggtat ctcgatgccc
     5101 agcgaaaccg ccagatccgc cgccatgacc gatggcccgc cgccgttggt gatcacggcc
     5161 agacggttgc cggccggtcg cttcggactg gtcagcgcgc gcgccgcgga aaacatgtca
     5221 ccgatggtct ggacacgaac gacgcccgcc cggcgcaacg ccgcgtcata cactttgtcg
     5281 tcccccacaa ccgggccggt ttgcgccaat gcaattgttt cactcgtctg agccttgccc
     5341 gccttgactg cgatgactgg tttgatgcgc gccgcagcgc ggacggcgct catgaagcgc
     5401 ctggaatcac gcaggttctc catatagacc agaatgcaat gggtattgat atccatcgcg
     5461 aggtaatcga tgatctcggc gaaatcgaga tcggctgcat tgcccagtga aacgatggaa
     5521 gaaaaaccga actcgttgtt gtaagaccaa tccagtatcg aggcgcagat cgtacttgac
     5581 tgcgaaatga gcgcgatatt cccgaccggc gcctgcttgg cgattggcgt caggttaagg
     5641 cccagtctcg ggcagaacag cccgaacgaa cgcggtccga tgatccgcac acggtatcgg
     5701 cgcgccacat ccagcatcgc gcgttcaatt cgcgcaccgc tggagccggc atcgcgaaaa
     5761 ccttccgaga acaggacgat ggcgcgcacg ccagccagac cacaggcttc aatgagcaag
     5821 ggcacttcag ccgctggcgc gataatcgca gcgaggtcga ccgtgtcccc catttccacc
     5881 tttacgtcgg ccagactggc atagcaggtg cggccgagga tactctcaag ctcgggatga
     5941 atcgggaata ccgcgccttt gtaactcacc aaaaggttct gcaacaaggc gtagccgatc
     6001 gaaccttctt cctcggtaac accgaatacc gcaatcgaag aaggcgaaaa caacttgcgt
     6061 agctgcaact ggctcatggt cttgagactc cctggttcaa gattcagaag acgtcagtcc
     6121 catcttgtag cgggtgcaaa cgtccttgta tgcatgttcg gacggcttcg gcgcagtgac
     6181 cgtgatgatc ctgccgtcac gcgtcagatg atagtccttt acaaacaaag tacaagcgca
     6241 tccaaacaac tgctcttggg tttccaggaa acctggcaat actggcgctg ctgacgttgc
     6301 atccgttatc attcacgtcg gtactcgcga taccatcaac gcaaggtgtg gtcgaccgtc
     6361 tgaatccgat actcgtaagt tgaacgttcg acacacaatt ctgatatgca aatgacggga
     6421 gaatgcattg tcgatactgt cg
//
LOCUS       DAORPM010000132         6434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3181_length_6434_cov_7.661389, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000132 DAORPM010000000
VERSION     DAORPM010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3181_length_6434_cov_7.661389"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLD53_07500"
     CDS             complement(<1..234)
                     /locus_tag="PLD53_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60796.1"
                     /translation="MGSLSNESFDEFLDSLKLAGVAISNERELRERLAEAQRWRFAFA
                     TLASNGRTLGIRFQDRSAGENEAKIRRAFNQFQF"
     gene            complement(448..876)
                     /gene="fliW"
                     /locus_tag="PLD53_07505"
     CDS             complement(448..876)
                     /gene="fliW"
                     /locus_tag="PLD53_07505"
                     /inference="COORDINATES: protein motif:HMM:NF009792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRC60797.1"
                     /translation="MKIDIERLGLKNVTIDPETLFSFPEGIAGFEDCRRFKVFHEEGK
                     TTVFWLQSVDDVAVAFPIVAPESLDIEYQIELSDADCALIDLQDAKDIAVAVIVYRDE
                     AEGGKIGANTRSPVILNLNSRKGMQKVLQSVHPTLLYRAR"
     gene            complement(968..2950)
                     /locus_tag="PLD53_07510"
     CDS             complement(968..2950)
                     /locus_tag="PLD53_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC60798.1"
                     /translation="MASAVTLKRILAARFFVLASVFSLSMMLVMLFWLKPRLQDLVTR
                     SNESLATALAVQAEKYLAAPQRTLEVLAERIVASEAGQQMPLTPLLDALVESSDFFEV
                     IYVVDRKGRIEHLGLPDTVKNRNDFVDLDLSGNPLVDRTRHGAKVGPSIWSNIFLSAV
                     SGRLAVALALSAGEHTVIGEVGLANLSAFVRRVANQEGTSMIIVDGQGQVLAHPDVEV
                     ARQQLNISYLPLLKAGEHSLGTAVTRFEGVEVIATRAPIAGIDWLVLVMQPLSIAREP
                     MITTLVVLSVVGLLATLLAIGLGIGFARRLAGVFERLVGTAESVAVGRYPGVWPRSRV
                     VEFGRLIDSLLRMSSAVREREEALASSQNQLRDLNQSLEQRVEERTAQLVSANAELQS
                     TLAQLSVAQDELQRAERLAALGALVAGVAHELNTPIGNCLVVATACAAKQREFEAEIE
                     HGLKRSSLNALVEGNRKAMDLLQVNLRRSAELISSFKQVAVDQTTVQRRQFSLHDTVH
                     EIVVTVSPTVKISGCRVVFDVPEDIRMESFPGPLGQVLSNLINNAVIHAYAGCATPEH
                     GEISIEARLLENDWVVLTVADKGEGIPPEHIKRVFDPFFTTRMGQGGTGLGLHIVYNI
                     VNDLLGGRIRVESEPGRGSRFIIQLPCVAPVSAIVV"
     gene            complement(2953..4056)
                     /locus_tag="PLD53_07515"
     CDS             complement(2953..4056)
                     /locus_tag="PLD53_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC60799.1"
                     /translation="MPRMSLSLVVLLVLLSACGEKEPLRIGFIGGLSGRFADLGTPGR
                     NGAMLAVELRNQQGGIGGRKIELLIRDDQQDPEVAKKGIDDLLAHQVKAIIGPMTSSI
                     AAAVVPRMNDARVLLMGATVTTASLSGRDDYFYRVVGTTATYASYAARYHYEKLGLRT
                     AALVLDVANLDYTESWVSEYAKTFESLGGKVVARVPFDSRADKSYLRIAGVLSEAKPE
                     IVALACSSVDGALIAQNLRKLDKSVRLAGSGWTATERLTELGGQAVEGMLVEQYFDRF
                     DESPRYKAFHKAFFDRFGQEPGFAGTTAYDAAMAVFAALEKDPSVKDLKKSLAEMKRF
                     QGVQGEIVFDEFGDASRKTYLNEVSRGQFVPVK"
     gene            4200..>6434
                     /locus_tag="PLD53_07520"
     CDS             4200..>6434
                     /locus_tag="PLD53_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC60800.1"
                     /translation="MKNTSSARKRETTDATDLVEFLDEGNDSGEEDDPRSPPSWHVLI
                     VDDDQAVHDATCYALNNTRIEGRPLHFLHAFSAGEALSLLNNRSDVAVLLLDVVMEQE
                     HSGLELVRTLRNDLNMHAMRIILRTGQPGYAPEAEVIRDYDINDYKLKSELTHAALVT
                     TLTSAIRSYVQICNILASQRGLSLIVDSATDLLAHLSLAEFAQKILPRLAELIGQAPE
                     GMLFCCTDQSTAKAGAANAKVVHATGAYAPWIDKPLEQIENQPVLSAVKQCLNDKGNV
                     YGKHAVTLFLGGAESRDAAIHFILQKPLSLTEKQLLDVFCVNVAIGLKNIGLFGDLHS
                     LAYSDPLTGLLNRIGFIEAIDKHASNQETGWTIALIDIDHFTEVNDALGHQRGDTLLI
                     GIARRLREYWGAGCTLARVGSDVFGLLAPEARLDPARLHEAFYEPIVVDGYLLPIEST
                     IGLTRMTDKCGNGLELFKTTSIALKHAKRVMRGRWLYHTPDMTASALERLDLTYKLRN
                     AINLKQGLHVHYQPQIELATGRLIGVEALLRWTTSDGTHVAPDRFIKLAEHAGLMVNL
                     GEWVFRTAVTQIANWDAAGLPPIRLAVNLSLMQFRDPFFVGRLRKIVNELKVPAHRLE
                     LEITESIAMLDTDSVITSLNEFKAMGMDVSIDDFGTGFSSLSYLHRLPLDRLKIDRSF
                     VQSMSSMGDSGSTIAHMVINLAHSLGLSVVAEGVESEDHALLLKTMGCEFGQGYLYSP
                     AMPPE"
BASE COUNT         1504 a         1952 c         1660 g         1318 t
ORIGIN      
        1 aaactggaac tggttaaaag cgcgacggat cttggcttcg ttctctccgg cgctgcgatc
       61 ctgaaagcgg atgcccagcg tgcgaccgtt ggatgccagg gtggcgaagg caaagcgcca
      121 gcgctgagct tcggcaagac gctcacgcaa ctcgcgttcg ttgctgattg caacacctgc
      181 cagtttcaga gaatccagaa attcgtcaaa ggattcatta ctcagactgc ccattttcag
      241 ctccgttggt ttcggtggat gcccaccatg agccgaataa cgcagcttct cgaaagaggt
      301 tgacacaaaa ttccaggaag cctggtggca atcactgccg tggtatcgat tcagcgccat
      361 tccctatgat tcaggaactg tcagttacgc ctaagagcga ccaacacaat ccacaagctg
      421 atgcgacaaa tgcaatccag cagatgctta tcgggcgcgg taaagcaatg tcggatgaac
      481 gctctgaagc accttctgca tccccttgcg actattcagg ttgaggatca ctggcgagcg
      541 tgtattggcc ccgatctttc cgccctcggc ctcgtcacgg tagacgataa ccgcaaccgc
      601 gatatccttg gcgtcctgca agtcgatcag cgcacagtcg gcatccgaga gttcgatctg
      661 gtactcgata tcgagcgact cgggagccac aatcggaaac gcgaccgcca catcatcgac
      721 ggactgcagc cagaaaacgg tggttttgcc ttcctcgtga aaaaccttaa agcgcctgca
      781 atcctcgaaa ccggcgatgc cctccggaaa gctgaacaag gtctccggat caatcgtgac
      841 attcttcaaa ccgaggcgtt cgatatcaat cttcatttaa gctcccttat aaatcccgaa
      901 aatccgtgca gcttcaattc atacatattg attcgaacaa agcaagcctt ccccgacagc
      961 acggcactca gacaacaata gccgaaacag gagcgacgca gggaagctga ataatgaagc
     1021 gcgaacctcg accgggttcg ctttcaaccc gaattctccc gccgagcaga tcgttgacga
     1081 tgttatacac aatatgcagc ccgagacccg ttccgccctg ccccatcctg gtcgtaaaga
     1141 aggggtcaaa aacccgcttg atatgctccg gtggaattcc ctcgcctttg tcggcaacag
     1201 tcagtaccac ccagtcgttc tcaagcaatc gcgcctcgat gctgatttcg ccatgttccg
     1261 gagtcgcaca tcctgcataa gcgtggatca cggcgttatt gatcagattg ctcagaacct
     1321 gaccgagcgg ccccggaaag ctttccatgc gaatgtcttc aggcacatcg aaaacaaccc
     1381 ggcaaccgct gatcttcacc gtcggggaaa ccgtgaccac aatttcatga accgtatcgt
     1441 ggagggaaaa ctggcgtcgc tgcaccgttg tctgatcgac cgccacttgc ttgaaactcg
     1501 aaatcagttc ggcagaacga cgaagattca cttgcagcag atccattgct ttccggtttc
     1561 cttcgaccag cgcattcagg gaagaccgct tcagtccgtg ttcgatctct gcttcgaatt
     1621 cacgctgttt cgccgcacag gccgtcgcaa cgaccaagca gttgccaatc ggcgtattga
     1681 gttcgtgcgc gacaccggca accaaagcac ctagggcggc gagacgctcg gcacgctgaa
     1741 gttcatcctg ggctaccgat aactgcgcga gggtagattg cagctcggca ttggcgctta
     1801 ccagctgggc cgtacgctct tcgacacgct gttcgagact ctggttgaga tccctgagct
     1861 ggttctgact gctggccagc gcttcttccc gttctcgtac ggcagatgac atgcgtaaca
     1921 aactatctat caatcggccg aactcgacca cacgggaacg cggccagacg cctggatagc
     1981 ggccaacagc caccgactct gcggtaccga ccagccgctc gaaaactccg gcaaggcgac
     2041 gggcaaatcc tatccccaag ccgattgcca gtagcgtcgc gagcaagcca acaaccgaca
     2101 aaacgaccag cgtcgtgatc atcggctcac gggctatcga cagcggctgc atcaccagta
     2161 ccaaccagtc aatcccggca atcggcgcac gcgttgcgat cacctcgaca ccctcaaagc
     2221 gcgtcacggc cgttccgagc gagtgttctc cggctttgag caaaggcaaa tagctgatgt
     2281 tcaattgctg gcgtgccacc tcgacgtccg gatgggcaag aacctgcccc tggccatcaa
     2341 cgatgatcat cgaggtgcct tcctggtttg ccacgcgacg aacaaacgct gacagattgg
     2401 ccagaccaac ctcgccgatc actgtgtgtt ctcccgcaga aagggccaga gcaaccgcca
     2461 ggcgaccgct gactgcggac agaaaaatat tcgaccagat tgacgggcca actttggcgc
     2521 cgtgtctcgt tcggtcaaca agcggattgc ctgacagatc gagatccaca aaatcatttc
     2581 ggttcttgac cgtgtcaggc agaccgagat gctcgattcg gcccttccgg tcgacgacgt
     2641 agataacttc gaagaaatca ctgctttcga ccagcgcgtc gagcaacggg gtaagcggca
     2701 tctgctgccc ggcctcgctg gcaacaatcc tttccgccaa cacctccagc gtgcgttgcg
     2761 gcgccgccag atatttctcg gcctgaaccg ccagtgcagt agccagtgat tcgttactcc
     2821 gggtcaccaa atcctgcaga cgcggcttca accagaaaag catgacgagc atcatgctca
     2881 aactaaaaac cgaggccaga acaaagaatc ttgcggcaag gatacgtttt agtgtaacag
     2941 cgctggccat acctatttga ccggaacgaa ctgcccgcga cttacctcgt tcagatacgt
     3001 tttgcgggag gcatcgccaa actcgtcaaa aacgatttca ccctgaacac cctgaaaacg
     3061 cttcatttcg gcgagtgact ttttcagatc ctttaccgag ggatcctttt caagcgctgc
     3121 aaaaacagcc attgccgcat cgtaggcagt ggtccccgca aaacccggct cctgtccgaa
     3181 acggtcgaaa aacgccttgt gaaacgcctt gtatcgcggt gactcgtcaa agcgatcaaa
     3241 atactgttca accagcatgc cctctaccgc ctgaccaccc agctcggtca gtcgctcggt
     3301 tgccgtccag cccgaaccag ccaggcgaac cgacttgtcc aatttgcgca ggttctgcgc
     3361 aatcagcgca ccatcgaccg agctgcacgc cagagcaaca atctcgggtt tggcttctga
     3421 caggacgccg gcaattcgca gatagctctt gtcagcgcgt gaatcgaagg ggaccctggc
     3481 gacaaccttc ccacccagcg actcaaaggt cttggcatac tcgctgaccc agctctcggt
     3541 gtaatcaaga ttagccacat cgagcaccag tgccgccgtg cgcaacccaa gcttctcgta
     3601 gtgatagcgt gccgcgtagc tggcgtaggt agccgtggtg ccgaccaccc ggtaaaaata
     3661 atcatcgcga ccggataatg atgccgtggt gaccgtcgcc cccatcagca gcacgcgcgc
     3721 gtcattcatt cttggcacga cagcggcggc gatgctgctg gtcatcggcc cgatgatggc
     3781 tttaacctga tgtgccagca aatcatcaat gccctttttc gccacttcgg gatcctgctg
     3841 gtcgtcccga atcagcagtt cgatcttgcg cccgccgata cccccctgct ggttccgcaa
     3901 ctccacggca agcattgcac cattgcgccc cggtgttcca agatcggcaa agcgtccgga
     3961 cagcccgccg ataaatccga tgcgcagggg ttccttctca ccacacgcag agagcagcac
     4021 aagcaaaaca accagactca ggctcatcct cggcatatca tcctccgatc aaacgtgttg
     4081 cggtcctgaa acaaagcgcg ccggacggat tggcagtgat tcgttccgcc ccatgaaaat
     4141 ctagactttg ccatcagaat cagtatattg aacatcatgc gaaaatgcag agattattca
     4201 tgaagaatac gagttccgcg cgtaagcgtg aaacgactga tgcaaccgat ttagtcgaat
     4261 tcctcgatga gggaaacgat tcaggcgagg aagacgaccc gcgctcgcca cctagctggc
     4321 atgtgctgat tgtcgacgat gatcaggccg tgcatgacgc cacctgctat gccctcaaca
     4381 acacacgaat agaggggcgt ccgcttcact ttctccatgc gttctccgct ggcgaagcac
     4441 tcagtctatt aaacaatagg agcgatgttg cggtactgct gcttgacgtc gtcatggagc
     4501 aggaacactc cggactggaa ctggtgcgca ccctgcgcaa cgatttgaac atgcacgcca
     4561 tgcgcattat tctgcgcacc ggccagcctg gctatgcacc ggaagccgaa gttatccgcg
     4621 actacgacat caatgactac aaactcaagt cggagctgac ccatgccgct ctcgttacga
     4681 cactgacttc ggcgattcgc tcctatgtgc agatctgcaa catcctggca agccagcgcg
     4741 ggttgtcgtt gattgtcgat tccgcgactg atctcctcgc acacctttcg ctcgccgagt
     4801 tcgcccagaa gatactgccg cgtcttgccg agctaatcgg tcaagcaccc gaaggcatgc
     4861 tgttctgctg taccgatcaa tcgacagcca aggcaggcgc agcgaacgcc aaggtcgtcc
     4921 acgcgaccgg cgcatatgcc ccgtggatcg acaagccgct tgagcagatt gaaaaccagc
     4981 cggtgctcag cgcagtcaag cagtgcctga acgacaaagg caacgtttac ggcaagcatg
     5041 cggtcacgct ctttctgggc ggagccgaaa gccgggatgc cgccatccat ttcattctgc
     5101 agaaaccgct gtcgctgacc gagaaacaac tgctggacgt attctgcgtc aatgttgcca
     5161 tcggcctgaa aaacattggc ctcttcggcg atttgcactc cctggcctac tcggatccgc
     5221 ttaccggtct gctgaaccgt atcggcttta ttgaagcgat agacaaacac gcttcgaacc
     5281 aagaaaccgg gtggacgatt gcactgatcg atatcgacca ctttaccgaa gtcaatgacg
     5341 cactcgggca ccaacggggc gacacactac tgatcggaat cgctcgccga ctgcgggagt
     5401 actggggcgc aggctgcacc ctggcgagag tcggctcgga tgtcttcggg ttgctggcgc
     5461 ccgaggcaag actcgacccg gcccggctgc acgaagcctt ttacgaaccc atcgtcgttg
     5521 atggttatct gctgcccatc gaaagcacca ttggcctgac ccggatgacg gataaatgcg
     5581 gcaacggtct cgaactattc aagaccacca gcattgccct gaagcacgcc aagcgtgtca
     5641 tgcgagggcg ctggctgtat cacacgcccg acatgacggc cagcgctctg gagcgactgg
     5701 atcttaccta caagctgcgc aatgccatca acctcaagca gggactgcat gtgcattatc
     5761 agccgcaaat cgaacttgcc actggccggc tgatcggcgt cgaagccctg ctgcgctgga
     5821 ccaccagcga cggtacgcac gtggcaccgg atcgcttcat caagctggcc gaacacgccg
     5881 gtctgatggt caatcttggc gaatgggtat tccgcactgc cgtcacccag attgcaaatt
     5941 gggatgcggc cggacttccg cccatccggc tggcggtcaa cctttcgctg atgcagttcc
     6001 gtgacccgtt ctttgtcggg cgcctcagaa aaatcgtcaa tgaattgaag gttcccgccc
     6061 atcgcttgga actcgaaatt accgaatcga ttgccatgct ggatacggat tccgttatca
     6121 ccagcctgaa cgagttcaag gcaatgggga tggatgtatc catcgatgat ttcggaaccg
     6181 gattttcgtc gctctcctac ctgcaccgac tgcctttgga ccgtctcaag atcgatcgaa
     6241 gcttcgtgca gtcgatgtca tcgatgggcg atagcggaag cacaatagcc cacatggtga
     6301 tcaatctcgc tcattcactg gggctgtcgg tcgttgctga aggcgtcgag tcggaggatc
     6361 atgccctgct gctgaaaacc atggggtgtg aattcggaca ggggtatctt tactcgccag
     6421 cgatgccgcc cgaa
//
LOCUS       DAORPM010000133         6414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3204_length_6414_cov_10.779840, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000133 DAORPM010000000
VERSION     DAORPM010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3204_length_6414_cov_10.779840"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLD53_07525"
     CDS             complement(<1..319)
                     /locus_tag="PLD53_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC60801.1"
                     /translation="MQDFLPIRYVEPVFRPPSEAQSLILPVTDGCSWNKCTFCEMYTA
                     PQKGFRARDEAEVLQSIQQTGEQYAEEIRRVFLADGDALVLSTRRLLTILQAIREHLP
                     AVRR"
     gene            complement(348..827)
                     /locus_tag="PLD53_07530"
     CDS             complement(348..827)
                     /locus_tag="PLD53_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRC60802.1"
                     /translation="MIACIFAFLLAACGSAPPSPRPSSDALARSEAYSRQGNEVTLFA
                     LGLIDTGYRFGGKNPEAGLDCSGMVSYIYNHAAGVTIKGSAADIARKGRPIERNELRA
                     GDLVFFNTRNFRFSHVGVYIGGDRFIHAPSSNGHVRIDQMSARYYAQRYEAARTFFE"
     gene            969..2420
                     /locus_tag="PLD53_07535"
     CDS             969..2420
                     /locus_tag="PLD53_07535"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRC60803.1"
                     /translation="MYLADPRLLRSNCFIDGRWEAADDGNVLPVNNPATGELIGEVPM
                     MGNTETLRTIVAANAAWKSWRETTAKDRAALLDRWFDLILANADDLARLITAECGKPL
                     AEARGEVGYGASFVQWFAEEAKRAYGESIPSPTAGKRLLTLRQPVGVAAAITPWNFPL
                     AMVTRKVAPALAAGCTIILKPAEQAPLSALALARLAAEAEIPAGVFNVLTGDPAIIGG
                     ALTASTLVRKLSFTGSTEVGRLLMAQCAPTIKKLSLELGGNAPFIVFDDADVDAAVEG
                     ALIAKFRNSGQTCVCANRFLVQAGVYDAFARKLAERVATLKVGAGDEDGVSLGPLIDD
                     AARVKVEEHIADAIGKGALLLTGGHHPTLGGNFFRPTILTDVTAEMRVAREETFGPVA
                     PIFRFTSEAEAIAQANDTESGLAAYFYSRDVARCFRVGEALEYGMVGINTGLISNEVA
                     PFGGVKQSGLGREGSKHGLDEYLEIKYLCFGIE"
     gene            complement(2474..3613)
                     /locus_tag="PLD53_07540"
     CDS             complement(2474..3613)
                     /locus_tag="PLD53_07540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRC60804.1"
                     /translation="MQTFVWGEEFYTGIGPVDEQHHGLVDLFNKLSASLTDTEMAGNT
                     DVQLAFSQLMDYTKYHFSNEAAIMQQEGVDQRHTATHLKLHGEFAEQVRAMWGARSTL
                     SNPAEFFLTFLTSWLCLHVLGVDQSLGRQVALIKSGMTPQQAYESELLRPKDKSAEAI
                     IKALRNTYRVVSRLSLDLISANQLLEEKVAARTAELQLANVALTTANSRLEAYSQTDG
                     LLGIANRACFDSRLANEWSRAIREQYPVGILMIDVDFFKNYNDHYGHQAGDTCLQAVA
                     KAVGGRIVRALDLLARYGGEEFVVILPNTSAQGAQKLALDMCDAVSGLNIPHTASTVA
                     DHVTVSIGVASILPDRHSSPDEAVAAADRALYAAKHQGRNQVCLA"
     gene            complement(3708..5162)
                     /gene="rng"
                     /locus_tag="PLD53_07545"
     CDS             complement(3708..5162)
                     /gene="rng"
                     /locus_tag="PLD53_07545"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HRC60805.1"
                     /translation="MPEDILINFTPQETRVAVMNQGAVQELHIERSSSRGLVGNTYVG
                     RIVRILPGMQSAFVDVGLDRTAFLHVADIWESRQQTEPVRPIERLLFEGQSLVVQVIK
                     DPIGTKGARLSTQISLAGRMLVYLPQDRHIGISQRIEKESDREALREKLAHLVGTNEA
                     GGFIVRTMAENATDAELANDIDYLRKIWHDIQFQAKTMEPPGLLFQELSLGQRVLRDF
                     VNPETARIVIDSRENFQKLSAFAAEYTPTVAPLLCHYTGENPLFDQFGVEDEIQRALA
                     RRVDLKSGGYLIIDQTEAMTTIDVNTGGFVGIRNFEDTIFKTNLEAAQTIARQLRLRN
                     LGGIIITDFIDMESEEHKNAVLSEFNRALEQDHTRLTVNGFTALGLVEMTRKRTRESL
                     AHVLCEECSTCQGRGEIKTARTVCYEILRELLRESRQFNAREFRVLGSVAVIDILLDE
                     EAQGLAMLSDFIGRPISVQAEPSYGQEQFDIVLM"
     gene            5237..>6414
                     /gene="yegQ"
                     /locus_tag="PLD53_07550"
     CDS             5237..>6414
                     /gene="yegQ"
                     /locus_tag="PLD53_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HRC60806.1"
                     /translation="MSDASALPQAELLAPAGSLAMMRTAFAFGADAVYAGQPRYSLRV
                     RNNDFGNLEMLGSAIGEAHAGGKQFYVVSNILPHNSKVRTYLADMAPVLALKPDALIM
                     ADPGLIDLAREAWPEMPVHLSVQANTVNFAAVRFWKKLGVSRIILSRELSLDEVAEIR
                     QECPDIELEVFVHGALCVAYSGRCLLSGYFNHRDSNQGSCTNSCRWDYKVEAGEEDAG
                     GNVRASASVAPKAAATTWLLEEKERPGELMPIEEDEHGTYIMNSKDLRAVEHVERLIG
                     IGVDSLKIEGRTKSPYYVARTAQVYRRAIDDAVAGCPFNPRLLGELEGLANRGYTDGF
                     FQRHHEHEMQNYLRGHSESGRSLYVGDVIAWDPARELAGVEVKNRFSVGDRIEVIHPA
                     G"
BASE COUNT         1256 a         1922 c         1940 g         1296 t
ORIGIN      
        1 cgcggcgtac cgccggcagg tgttcgcgga tcgcttgcag tatggtcagc agacggcggg
       61 tggagagcac cagcgcgtcg ccgtcggcga gaaagacgcg gcgaatttct tcggcgtact
      121 gctcgccggt ttgctggatg ctttgcaata cctcggcctc atcgcgtgca cgaaacccct
      181 tctgcggcgc cgtgtacatc tcgcaaaagg tgcacttgtt ccacgagcag ccgtcggtca
      241 ccggcaggat cagcgattgg gcttcgctcg gcggtcggaa gaccggttca acgtagcgga
      301 tgggcaggaa atcttgcaat cgggtttggg cctgagaata tcaacggtca ttcaaagaag
      361 gtacgagccg cttcgtagcg ttgggcgtag tagcgggcac tcatctggtc gatgcgcaca
      421 tgaccattgc ttgagggcgc atgaatgaag cggtcgccgc cgatgtaaac gccgacgtgc
      481 gaaaagcgga agttgcgcgt attgaagaag accagatcgc ctgcgcgcaa ttcgttgcgc
      541 tcgatgggtc gacccttgcg ggcaatgtcg gcggcactgc ctttgatggt cacgccagca
      601 gcatggttgt agatgtagga caccatgccg ctgcaatcca gcccggcttc cggattcttg
      661 ccgccgaagc ggtaaccggt atcgatcaaa cccagcgcga acagcgtgac ttcatttcct
      721 tgcctgctat acgcctcgct cctggccagc gcatccgacg acggccgggg cgagggcggc
      781 gcactgccgc aggcggccag cagaaatgcg aaaatacagg cgatcaatgt gtgtatctgc
      841 atgccgtgaa gtataaactg tgatcagaac ccgtccacct gaaaaaggtg ggacttcccg
      901 acgatgcgca aagctgtcat ggctcaatcg gggcaaattc atccactcac tgaatcacta
      961 cacgacctat gtatcttgcc gaccccagac tacttcgttc caactgtttt atcgacggcc
     1021 gctgggaggc ggcggatgac ggcaacgtac tgccggtcaa caaccctgcg accggtgaat
     1081 tgatcggtga agtgccgatg atgggcaaca ccgaaacgct gcgcaccatc gtagcggcaa
     1141 acgcggcctg gaaaagctgg cgcgaaacca cggcgaaaga tcgggctgcg ctcctcgacc
     1201 gctggttcga cctgattctt gccaacgccg acgatctggc ccgcctgatc accgccgagt
     1261 gcggcaagcc gttggccgag gcgcgcggtg aagtcggcta tggcgcgtcc ttcgtccagt
     1321 ggttcgccga agaagccaag cgtgcctatg gcgagagcat tcccagtccg accgccggca
     1381 aacgactgtt gacgctgcgt cagccggtgg gtgtcgccgc cgccatcacg ccgtggaatt
     1441 tccccctggc gatggtcacg cgcaaggtcg ctccggcgct ggccgccggc tgcacgatca
     1501 ttctcaaacc ggccgaacag gcgccgctca gcgcgctggc actggcccga ctggcggccg
     1561 aagcggaaat accggccggc gtcttcaacg tgctgaccgg cgacccggcg atcatcggcg
     1621 gtgcgctgac cgccagcaca ctggtgcgca aactgtcctt taccggatcg accgaggttg
     1681 gtcgcctgtt gatggcgcag tgcgcgccga cgatcaagaa gctgtcgctt gagctcggcg
     1741 gcaatgcgcc cttcatcgtg ttcgacgacg ccgatgtcga cgctgccgtc gaaggcgcgc
     1801 tgattgccaa gtttcgcaat tccggacaaa cctgcgtttg cgccaaccgc tttctcgtcc
     1861 aggcgggtgt ctacgacgct tttgcccgga agctggcgga acgcgtcgca acgctcaaag
     1921 tcggcgcggg tgacgaagac ggcgtatcac tggggccgtt gatcgacgat gccgcacggg
     1981 tcaaggtcga ggagcacatc gccgatgcca tcggcaaggg cgcgctgttg cttaccggtg
     2041 gacaccaccc taccctgggt ggcaacttct tccggccgac gatactcacc gatgtcaccg
     2101 cggagatgcg cgttgcgcgc gaagaaacct tcggtccggt ggcgccgatc ttccgcttca
     2161 ccagcgaggc cgaggcgatc gcccaggcca acgacacaga atccggactt gccgcgtatt
     2221 tctactcgcg cgacgtcgcc cgctgctttc gcgtcggaga agcgcttgaa tacggcatgg
     2281 tcggaatcaa caccggactc atctcgaacg aggtcgcgcc cttcggcggc gtcaagcaat
     2341 ccggtctggg acgcgaaggc tcgaagcacg gactcgacga gtatctggag atcaagtatc
     2401 tgtgcttcgg catcgaatga ccggaatgag ccgcctgccg ggctgcttca tgtacttctc
     2461 acgatgcggc gtttcaggcg agacagacct ggttcctgcc ctggtgcttc gcggcataaa
     2521 gggcacggtc ggcagccgcc acggcctcat ccggagatga atgccgatcg ggcagtatcg
     2581 atgccacgcc gatactgacc gtaacgtgat ccgccaccgt cgatgccgtg tgcggaatgt
     2641 tcagaccgga tacggcatcg cacatgtcaa gcgcaagctt ctgggcgcct tgtgccgacg
     2701 tattgggcag gatcacgacg aattcttcac cgccgtagcg cgccagcagg tccagcgccc
     2761 gcacgatcct gccgccaacc gctttggcga ctgcctgcag acaggtatca cccgcctgat
     2821 gtccgtagtg gtcgttgtaa ttcttgaaaa aatcgacatc gatcatcaat atacccaccg
     2881 gatactgctc gcgaatcgca cgactccatt cgttggccag acgggaatcg aaacacgccc
     2941 gattggcgat gccgagcaga ccgtcggtct gggaatacgc ttcgagcctg ctgttggctg
     3001 ttgtcagcgc cacgttggcc agttgcagct cggcggttcg cgcggcgacc ttctcttcaa
     3061 gaagctggtt cgccgaaatc agatcgaggc tgagccgtga aaccacccgg taggtgttgc
     3121 gcagcgcctt gatgatggct tccgcgctct tgtccttcgg cctgagcaac tccgactcgt
     3181 aggcctgttg cggcgtcatg ccggatttga tcagcgcaac ctgtcgcccc aacgattggt
     3241 ccacacccag aacgtgcaag caaagccagg acgtgagaaa ggtcagaaag aactccgccg
     3301 gattgctcaa ggtggatcgt gctccccaca tggcgcgcac ctgctcggcg aactctccgt
     3361 gcagcttgag atgcgtcgcg gtgtggcgtt gatcgacacc ctcctgctgc ataatggccg
     3421 cttcgttgga gaagtggtat ttcgtgtagt ccatcaactg tgaaaaggcg agctggacgt
     3481 cggtgttccc cgccatttcg gtgtcggtca gcgacgcgct cagcttgttg aatagatcaa
     3541 ccagcccgtg atgctgctca tcgacgggtc cgatgcccgt atagaattct tcgccccaaa
     3601 caaaggtttg catgctttgg tctcaggtag ttttgcgatg cctacgcctt aggttggcaa
     3661 tataccggga aatcaaggcc aaaagaccca gagtccagca tgctttttta catcagaacg
     3721 atatcgaatt gctcctgccc ataactgggc tcggcctgca ccgaaatcgg tcggccgatg
     3781 aagtcggaca gcatggccag cccctgtgct tcctcgtcga gaagaatatc gattactgcg
     3841 acacttccca gaacgcgaaa ctcccgggca ttgaattggc gcgattcgcg caggagttcg
     3901 cgcaggatct cataacatac ggtgcgggca gtctttattt cgccacgacc ctgacaggtc
     3961 gagcattctt cacacagcac atgcgccaga gattcgcggg tgcgcttgcg tgtcatttcg
     4021 accagcccca gcgcagtgaa gccgttgacc gtcaaacgcg tgtggtcctg ctcaagcgcc
     4081 cggttgaatt cgctgagtac cgcgtttttg tgctcctcgc tttccatgtc gatgaagtcg
     4141 gtgatgatga tgccaccgag gttgcgcagg cggagctggc gggcaatcgt ctgcgccgct
     4201 tcgagattgg tcttgaatat ggtgtcttcg aagttgcgaa ttccgacgaa tccgccggtg
     4261 ttgacgtcga tggtggtcat cgcctcggtc tgatcgatga tcagataccc gcccgatttg
     4321 agatcgacgc ggcgagccag cgcccgctga atctcgtctt ccacgccgaa ctggtcaaac
     4381 aggggattct cgccggtgta atgacagaga aggggggcaa cggtcggtgt gtattcggcg
     4441 gcgaaagcac tgagtttctg gaagttttcc cgtgagtcga taacgattcg agccgtctct
     4501 ggattgacga aatcgcgcag cacacgctgg ccgagcgaca gttcctgaaa aagcagcccg
     4561 ggcggctcca tcgttttcgc ctgaaactga atgtcatgcc agatcttgcg caggtaatcg
     4621 atatcgttgg ccagttcggc atcggtcgca ttttccgcca tcgtgcggac aatgaagccg
     4681 cccgcttcgt tggttcccac cagatgcgcg agtttttcgc gcagcgcttc gcgatcggac
     4741 tctttctcga tgcgctggga aatgccgata tgccggtcct gcggcagata caccagcatc
     4801 cggccggcca gcgagatttg cgtcgacagt cgcgctccct tggtgccgat cggatccttg
     4861 atgacctgta cgaccaggct ttggccttcg aacagcaggc gctcgattgg ccgcacgggt
     4921 tccgtttgct ggcgcgattc ccagatgtcg gcgacgtgca ggaaggccgt gcggtcgagg
     4981 ccgacatcga cgaaagccga ctgcatgccg ggcagaatgc gaacaatccg gccgacatag
     5041 gtattgccga ccaggccgcg gcttgaactg cgttcgatgt gcaattcctg cacggcacct
     5101 tgattcatga cggcaacccg ggtttcctgc ggcgtaaagt tgatcaggat atcttcgggc
     5161 atgggcggac gattcggaga aagcaggtaa aatcggaaat tcgcagcatt ctaccccttt
     5221 cccggtattc gctcccatgt cagacgcctc tgcgttgcct caagccgagt tgcttgcacc
     5281 cgccggttcg ctggccatga tgcgcacggc ttttgccttt ggcgccgacg cggtctatgc
     5341 cggccagcca cgctacagcc tgcgtgtacg caacaacgat ttcgggaatc tggaaatgct
     5401 gggcagcgcg atcggtgaag cgcatgctgg cggcaagcaa ttctacgttg tcagcaacat
     5461 ccttccgcac aactccaagg tgcgcaccta tctggcagac atggcgccgg tgcttgcact
     5521 caaacccgat gccctgatca tggccgatcc cgggctgatc gacctggcgc gggaggcctg
     5581 gcccgagatg ccggtgcatc tctcggtgca ggccaatacc gtgaactttg ccgccgtccg
     5641 tttctggaag aagttgggcg tttcgcgcat cattctttcg cgcgaactgt cgctcgatga
     5701 agtcgccgaa atccgtcagg aatgtccgga tatcgaactc gaagtcttcg tgcacggcgc
     5761 gctgtgcgtt gcttattccg gccgttgttt gctgtccggc tacttcaacc accgtgattc
     5821 gaaccagggg tcgtgtacca actcctgccg ctgggactac aaggtggaag caggcgaaga
     5881 agatgccggt ggcaatgtgc gcgccagtgc ctcggtcgcg cccaaggcag cggcgaccac
     5941 ctggctgctc gaggagaagg agcgtcccgg cgagctgatg ccgatcgagg aagacgagca
     6001 cggtacctac atcatgaatt cgaaggacct gcgcgccgtc gaacacgtgg aacgcctgat
     6061 cgggatcggc gtcgattcgc tgaaaatcga agggcgcacc aagtcgccct attatgtggc
     6121 gcgcacggca caggtctatc gtcgcgccat cgacgatgcc gtcgccggct gcccgttcaa
     6181 cccccgactt ttgggcgaac tcgaagggct ggccaaccgt ggctacaccg acggtttctt
     6241 ccagcgccat cacgaacacg agatgcagaa ctatctgcgc ggccactcgg aatccgggcg
     6301 cagtctctac gttggcgacg tgatcgcctg ggatccggca agagagctgg caggcgtcga
     6361 ggtgaagaat cgtttttcgg tcggcgaccg catcgaggtc atccacccgg ccgg
//
LOCUS       DAORPM010000134         6404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3215_length_6404_cov_10.105528, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000134 DAORPM010000000
VERSION     DAORPM010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3215_length_6404_cov_10.105528"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..3095)
                     /locus_tag="PLD53_07555"
     CDS             complement(102..3095)
                     /locus_tag="PLD53_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HRC60807.1"
                     /translation="MPVSFLSNEQRESYGRYTGAPSLDDLARYFHLDDSDLALIAQKR
                     GEHNRLGFAVQLCTVRYLGTFLEDPLTVPTQVLHTLAKQLRIAIIPDAHLYSASRQRL
                     QHAAEIQTHYGYVEITERQIGFHLTRWLYAICWTGTDRPTVLFERAVTWLVTHKVLLP
                     GSSTLERYIARLRSRVEERLWLALGRGISSEQQGRLESLLLVPLGSRSSPLDQLRKGP
                     VMVSSRSLVIALMRLHSVRELGIRLPAAARIPATRVAALARFAGAAKVSAILRLPNPR
                     RLATLVAFVHCLEASAQDDGLEVLEVLLRELFGDAIKADKKSRLRTLKDLDQAAATLA
                     SACQMLLDTSLPDGDLRTRLFEKIPRNALTLALEGVNALIRPADNVYYQELDAKYKTV
                     RCFLPALVEHIRFGANVAGEPVVAAFDWLRSNIARKKPGNDAPREVIRKPWQRHVLRE
                     DGSVDFHAYTFCVLDELHTALRRRDVFVAPSWRYADPRAGLLDGAEWEATRPIICRTL
                     GLSAQPEPTLSALTNELDRTYRAVAARLPDNPAVRFDQVGDKQELVLSPLDKLDEPAS
                     LIALRERVAGMLPRVDLPELILEVAARTGFTDAFTHLSERTARATDLPISLCAVLMAE
                     ACNTGPEPLIRGDIPALKRDRLSWVDQNYVRDDTLTAANAILVAAQSRMALANLWGGG
                     EVASADGIRFVVPVRTVHAGPNPKYFGVGRGVTWYNLISDQFSGLNDITVPGTLRDSL
                     ILLAVVLEQQTDLQPTQIMTDTGAYSDVVFGLFRLLGYRFSPRLADVGGTRFWRIDPQ
                     ADYGLLNTISAHNLSLQKIIPHWDDMLRLVGSLKLGLVPATGIMRTLQVGDRPTRLAQ
                     AIAEFGRIDKTLHTLTYIDDEAKRRGTLTQLNRGEGRHSVARAVFHGKRGELRQHYRE
                     GQEDQLGALGLVLNMIVLWNTIYMEAALDQLRADGYTVKNEDVARLSPLLHEHINMLG
                     RYSFSVPEAVAKGELRPLHDPADND"
     gene            complement(3125..3742)
                     /locus_tag="PLD53_07560"
     CDS             complement(3125..3742)
                     /locus_tag="PLD53_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRC60808.1"
                     /translation="MNIARIYLRVSTEEQDLTRQAEIELSTRAAGYYIAGVYREKASG
                     ARADRPELLRMIADLQPGEVVVAEKIDRISRLPLAEAEQLVGSIRAKGARLAVPGLVD
                     LSDFAAEADGVAKIVLESVQELLLKLALQMARDDYETRRERQRQGVQLAKASGKYKGR
                     KPDGPTHHRIVALRGAGHTIQKTAELAGCSTSQVKRIWAMHQGKP"
     gene            3917..4297
                     /locus_tag="PLD53_07565"
     CDS             3917..4297
                     /locus_tag="PLD53_07565"
                     /inference="COORDINATES: protein motif:HMM:NF018050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRC60809.1"
                     /translation="MRVFKTRVFDRWAGKAGVTDTALLKAVSDIERGLIDADLGSNLF
                     KQRVALPGRGKSGSTRTLLATRFAGTLYFLFGFEKKDRDNITQRELGIYQSLASTLVV
                     LSNDQIETALKANELMEVQHEPND"
     gene            4281..4580
                     /locus_tag="PLD53_07570"
     CDS             4281..4580
                     /locus_tag="PLD53_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRC60810.1"
                     /translation="MSRMINEALETMRGLHDIGAVNKQTLRDFETQCLPPPAYTAEMI
                     RRLRESLHISQAVFAAYLNASISTVQKWENGDKKPSGAAARLLSVIERKGLEVLV"
     gene            complement(4812..5579)
                     /locus_tag="PLD53_07575"
     CDS             complement(4812..5579)
                     /locus_tag="PLD53_07575"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRC60811.1"
                     /translation="MNKNINLNADLGESFGAWSMGDDESLLDIVASANIACGFHAGDP
                     LVMRRTVAAAKKRGVSIGAHPAFPDLQGFGRRRMDIPAAELEAMIIYQIGALKAMAAA
                     EGSCVSHVKPHGALSNMACADPALADCVARAVHAISPQLILLAPAYSRLATAGERIGL
                     PVILEIFADRAYQDDGQLMPRSQPGAMIHGTDAALAHVLRMLDEQAIVSVNGKRLPTR
                     VQSICVHGDNPEAVATASDLRRGIEAADYSIVSLDRL"
     gene            complement(5596..>6404)
                     /locus_tag="PLD53_07580"
     CDS             complement(5596..>6404)
                     /locus_tag="PLD53_07580"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRC60812.1"
                     /translation="TNEQSMAWMMDTYSMNVGGTATGVVTGKPIPLGGSLGRHEATGR
                     GVFIAAREAGKRIHLPVEGARVVVQGFGNVGGIAARIFQEHGAKVIAVQDHSATLANE
                     NGIDIHAALKHVEINGGLAGFPGASPISGDDFWLLKSDFLIPAALEGQILPERAQKIS
                     TKVVVEGANGPTTPAADDILQQRDILVVPDVLANAGGVTVSYFEWVQDFSSFFWTEEE
                     INARLEQIMVGAFDAIWKLSEERNVSLRTATFIIACQRVLAAREQRGLYP"
BASE COUNT         1260 a         1898 c         2000 g         1246 t
ORIGIN      
        1 atctgcaggc cgcgccagtg tttgcgcctc tgtgacgctg gctgcgaagg tgtccggggt
       61 ttggggtgca gaggaacggc agaagcagtt aagcgtttgt tctaatcgtt atcagcggga
      121 tcatgcaggg gacgcaattc gcctttggca acggcttccg gcaccgagaa cgaataccgc
      181 cccagcatgt tgatgtgctc gtgcagcagc ggtgacaacc gcgccacgtc ttcattcttc
      241 accgtgtagc cgtcagcgcg tagttggtcc agggcggctt ccatgtagat cgtgttccac
      301 aacacgatca tgttcagcac caaaccgagc gcacccaatt ggtcttcctg cccttcgcgg
      361 taatgctggc gcaactcgcc gcgcttgcca tgaaacacgg cccgcgcaac gctgtggcgc
      421 ccttcgcccc ggttaagctg tgtcagcgta ccccggcgct tggcttcgtc atcgatgtag
      481 gtcagcgtgt gcaacgtctt gtcgattcga ccgaattccg cgatagcctg cgccagtcgg
      541 gttggccgat caccgacctg gagagtgcgc atgatgccgg tggccggcac cagaccgagc
      601 ttgagtgatc caaccaggcg cagcatgtcg tcccaatggg gaatgatctt ttgcaagcta
      661 aggttgtgcg ccgaaattgt gttgagcagg ccgtagtcag cctgcgggtc gatgcgccag
      721 aagcgcgtgc cgccgacatc ggccagtcgc gggctgaagc gatagccgag caggcggaac
      781 aggccgaaca ccacgtcgct gtaagcaccg gtatcggtca tgatctgggt aggctgaaga
      841 tccgtctgtt gttcaagcac cacggccagc aggatcagac tatcgcgtaa ggtgcccggt
      901 acggtgatgt cgttcaggcc agagaactgg tcggagatca ggttgtacca agtgacgccg
      961 cgaccgacgc caaagtattt gggattcggg ccggcatgca ccgtgcgcac cggcaccacg
     1021 aaacggatac cgtcggctga tgccacctcc ccgccgcccc acaggttggc caacgccatg
     1081 cgactttgag ccgccaccag tatcgcgttg gcggccgtca gcgtatcgtc gcgcacataa
     1141 ttttggtcta cccaggacaa gcggtcgcgt ttgagcgccg ggatgtcgcc gcgaatcagc
     1201 ggctccggcc cggtattgca ggcttcggcc atcagcaccg cgcacaagct gatagggaga
     1261 tcggtggcgc gggccgtacg ctccgagagg tgggtgaagg cgtcggtgaa gccggttcgc
     1321 gcagccacct caagaatcag ttcgggcaag tccacgcgcg gcagcatgcc ggcgaccctc
     1381 tcccgcaatg cgatcagcga agccggttca tccagcttgt ccaatggact gagtaccaac
     1441 tcttgtttgt cgccaacctg atcgaaacgt accgccgggt tgtcgggcaa ccgagcggct
     1501 acggcgcgat aggtacgatc cagctcgttg gtcaaggcgc tcaacgtcgg ctcgggttga
     1561 gccgacagtc ccaacgtccg gcagatgatc ggccgggtgg cttcccattc cgcgccgtca
     1621 agcaggccag cacgaggatc ggcatagcgc caactgggcg ccacgaagac gtcgcgccgg
     1681 cgtaaggcgg tgtgcaactc atccagcacg cagaaggtgt aggcatggaa atcaacgctg
     1741 ccatcctcgc gcagaacatg tcgctgccac ggttttctga tcacctcacg cggagcgtcg
     1801 ttacccggtt tcttgcgggc gatgttgctc cgcagccaat caaaggcagc aacaaccggc
     1861 tcgccagcca cattcgcacc gaaacgaatg tgctcgacca aggccggcag gaaacaacgc
     1921 accgtcttgt acttggcgtc cagctcctgg taatacacgt tgtcggccgg tcggatcagc
     1981 gcattgacgc cttcgagcgc caacgtgagc gcgttacgtg gaatcttctc gaacaatcgg
     2041 gtgcgcagat cgccatccgg caaactggtg tccagcaaca tctggcaggc gctggccagc
     2101 gttgccgccg cctgatcgag atccttgagc gtgcgcaggc gcgacttctt gtccgccttg
     2161 atggcgtcgc cgaacaattc gcgcaggagc acctccagca cttccagtcc atcgtcctgc
     2221 gcgctggctt ccaggcaatg cacgaaggcg accagcgtgg ccaagcggcg cggattcggc
     2281 aggcgcagga tggcgctgac cttggcggca cctgcaaacc gggccaaggc cgccacacgg
     2341 gtcgccggaa tgcgggcggc tgcgggcagc ctgataccca gttcgcgcac cgagtgcagt
     2401 cgcatgagcg cgatgaccaa ggatcggctg ctcaccatga ccggcccttt gcgcaactga
     2461 tccaggggcg aactccggct gccgagagga acgagcagca ggctttccag ccgtccctgc
     2521 tgttcactgc tgatgccccg gccgagcgcg agccacagac gctcctccac ccggctgcgc
     2581 aggcgggcga tatagcgttc gagtgtgcta ctgccgggta gcagcacctt gtgcgtgacc
     2641 aaccaggtca cggcccgctc gaacagcacg gttggccggt cggtgccggt ccagcaaatc
     2701 gcgtagagcc agcgggttag gtggaagccg atctggcgct cggtgatttc gacatagccg
     2761 tagtgggttt gaatttctgc ggcatgctgc aagcgctgcc tgcttgcgct gtagagatgg
     2821 gcatcgggga tgattgcgat gcgcaactgc ttggccagcg tatgcagtac ctgcgtcggt
     2881 acggtcagag ggtcttccag gaaagtgccg agataacgca ccgtgcagag ctgcacggcg
     2941 aaacccagcc ggttgtgttc accgcgtttc tgcgcgatca atgcgaggtc gctgtcgtcg
     3001 aggtggaagt aacgggcgag gtcgtcgagt gatggcgctc cggtgtatcg gccatagctt
     3061 tcgcgctgtt cgttggagag aaagctgacc ggcatgagtg ctcctgtaga ggtgccacaa
     3121 cccgttacgg cttgccttgg tgcatggccc agattcgctt gacctggctg gtgctgcatc
     3181 cagccaactc ggcagttttc tggatcgtat gaccagctcc ccgcaaggcc acaatgcggt
     3241 gatgggtggg gccatccggc ttccgcccct tatattttcc ggaagccttg gcaagttgca
     3301 cgccttggcg ctgccgctca cggcgggttt cgtaatcgtc gcgtgccatt tgcagggcca
     3361 gcttaagcag gagttcctga actgactcca gcacgatctt ggccacgcca tcggcttcgg
     3421 cggcgaagtc agagagatca accaaacccg gcacggccag ccgggcgcct ttggcccgga
     3481 tcgagccaac caattgctca gcctcggcca aggggagtcg gctgatgcgg tcgatcttct
     3541 cggcaactac cacctcgccc ggttgcaggt cagcgatcat ccgcagcaat tcgggccggt
     3601 cggcgcgggc acccgacgcc ttctcacggt agacgccagc gatgtaatag ccggccgccc
     3661 gcgtgctgag ctcaatctcc gcctgtcgag tgagatcctg ctcttcggta ctgacgcgaa
     3721 ggtaaatgcg ggcgatgttc atgggtgctg gccaaaatgg gtatacggaa cttggctaac
     3781 tatagacggt atggccaata aggcaaaaca cgatgtaaat tgtccacaac caatttagct
     3841 attcatattg gccagatcga cgccagagta gcgtcggcca ttgacttcag tgttgcacca
     3901 agtgatacaa tgagtcatga gagttttcaa gacacgggta tttgatcgtt gggctgggaa
     3961 agctggcgtc accgacaccg ctttgttgaa ggccgtttca gatattgaac gcgggctgat
     4021 tgatgcggat ctgggtagca acctgttcaa gcaaagggtg gcgctacctg gacgaggcaa
     4081 aagcggtagc acgcgcactt tgctggccac acggttcgct ggcacgttgt actttctttt
     4141 cgggtttgag aagaaagatc gcgacaacat cacccagcgc gagttgggga tttaccagag
     4201 cctcgcgagc acattggtcg tgctcagtaa cgatcagatt gaaaccgcgt tgaaagccaa
     4261 cgaattgatg gaggtgcagc atgagccgaa tgattaacga agccctggaa acgatgcgtg
     4321 gtttgcatga catcggcgca gtgaacaagc aaacattgcg tgactttgaa acacaatgcc
     4381 tgccaccgcc ggcctacacg gcggaaatga tccggcgctt gcgggagagc ctgcacatca
     4441 gtcaggccgt ctttgctgcc tacctcaacg ccagtatctc taccgttcag aagtgggaaa
     4501 acggagataa aaaacccagt ggggctgcgg cacgattgct ttcagtgatt gaacgcaagg
     4561 ggctggaagt gctggtttga acaagctgcc tgtagcgaga gacgcagcaa ctgatcaaat
     4621 tttggtgaag tcattcgtca tttttctatg atcggcagca ttcggccaca accggccggt
     4681 gatcacgtcc acgaagcggc cactcgaagg tccgccgtac tccgatatct gccgtcggat
     4741 cgaatgacac tacaatgtag tgcccccgct cgcggacggg ggccggttaa ctgcagtatt
     4801 ttccccctgc cttacagtcg atcgaggctg acgatgctgt agtcggccgc ctcgatgccg
     4861 cgtcggagat cgctggcggt ggcaaccgcc tccgggttgt cgccgtgaac gcaaatgctt
     4921 tgcacgcggg tcggtaggcg cttgccgttg acgctgacga tggcttgttc atccagcatg
     4981 cgcaggacgt gggcgagtgc ggcgtcagtg ccatggatca tggcgccggg ctggctgcgt
     5041 ggcatcaact ggccgtcgtc ctgataggca cgatcggcga aaatctcaag gatgaccggc
     5101 aggccgatgc gttcgccggc tgtagcgagt cgggagtagg ccggggccag cagtatcagt
     5161 tgcgggctga tggcatggac ggcacgagcg acgcaatcgg ccagcgccgg atcggcacag
     5221 gccatgttgc tgagggcgcc atgcggcttg acgtgactga cgcagctgcc ttcggccgcg
     5281 gccatcgcct tcagggcgcc aatctggtag atgatcatcg cttccagctc ggcagccggg
     5341 atatccatgc gccgccggcc gaagccttgc agatcgggga acgccgggtg ggcgccaatg
     5401 ctgaccccgc gctttttggc cgccgcgact gtcctccgca tgaccaaagg atccccggca
     5461 tgaaagccgc aggcaatatt ggcgctggcg acgatgtcga gcagggactc gtcatccccc
     5521 atggaccacg caccgaagct ttcaccgagg tcggcattga gattgatgtt tttgttcatg
     5581 agcaggttcc ccaggttaag gatacaagcc gcgctgttcg cgcgctgcga gtactcgctg
     5641 acaggcaatg atgaaggttg cggtgcgcag cgacacattg cgctcctcgg acagtttcca
     5701 gatcgcgtcg aaggcgccga ccatgatctg ctcgagacgg gcattgatct cttcttcggt
     5761 ccagaagaac gaggagaaat cctgtaccca ctcgaagtac gacaccgtta cgccgccagc
     5821 gtttgccagc acgtcgggaa cgaccagaat gtcgcgctgc tggaggatat cgtcggccgc
     5881 cggggtggtc gggccgttgg cgccctcgac cacgactttg gtgctgatct tctgtgcccg
     5941 ctcgggcagg atctggccct caagggcggc cggaatcagg aagtcagact tgagcagcca
     6001 gaaatcgtcg cctgaaatcg gcgacgcgcc cgggaagccg gccaggccgc cattgatctc
     6061 cacatgcttg agcgcggcgt ggatgtcgat gccgttttca ttggccagcg tcgccgaatg
     6121 atcctgcacc gctatcactt tggcgccgtg ttcctggaag atgcgagccg cgataccgcc
     6181 gacgttgcca aaaccctgaa ccacgacacg ggcaccttcg accggcaggt gaatccgttt
     6241 accggcttca cgggcagcga tgaacacgcc gcgaccggtg gcttcgtgac ggccgagtga
     6301 gccgccaagg ggaatcggct tgccggtgac gacgcccgtc gcggtgccac cgacgttcat
     6361 cgaataagtg tccatcatcc acgccatgct ctgctcgttg gtgc
//
LOCUS       DAORPM010000135         6337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3273_length_6337_cov_21.134352, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000135 DAORPM010000000
VERSION     DAORPM010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3273_length_6337_cov_21.134352"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="nuoG"
                     /locus_tag="PLD53_07585"
     CDS             complement(<1..939)
                     /gene="nuoG"
                     /locus_tag="PLD53_07585"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRC60813.1"
                     /translation="MLDIEIDGKQVQVPAGGTIMDAAQLVGAYIPHFCYHKKLSISAN
                     CRMCLVQVEKAPKPLPACATPVTSGMKVFTHSELAVNAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGSASRYQEAKRVVFHKDVGPLVSMQEMARCIHCTRCVRFGQEI
                     AGVMELGMANRNMHSEIQTFVGRTVDSELSGNMIDICPVGALTSKPFRYSARSWELSR
                     RKSVSPHDSLGSNLTVQVKSDRVMRVLPRENEEINECWISDKDRFSYEALNSSDRLQK
                     PMIRVDGVLREVEWNVALDYVSHGLKDIASQHGGEQI"
     gene            complement(949..2283)
                     /gene="nuoF"
                     /locus_tag="PLD53_07590"
     CDS             complement(949..2283)
                     /gene="nuoF"
                     /locus_tag="PLD53_07590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRC60814.1"
                     /translation="MAILGAINPMLTVGLDYESDRAWRLDGYVERGGYSALKRILAEK
                     MPQDQVIGEVKKSVLRGRGGAGFPTGLKWSFMPRAFPGDKYVVCNSDEGEPGTFKDRD
                     ILLFNPHSVIEGMAIAAYAMGASRGYNYIHGEIWEVYTRFEDALDEARAAGLLGKDIL
                     GSGFDFDLFAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASYGLYGKPTTINNT
                     ETFGAIPFILELGGEAYLEAGKANNGGTKLFSISGHVNKPGNYEIPLGTPFSTLLEMA
                     GGMRGGRQLKGCIPGGSSSPVVPGDIMMECTMDYDSISKAGSMLGSGAVIIMDETTCM
                     VRALERLSYFYYEESCGQCTPCREGTNWLYRIVHRIESGQGRPEDLDLLNSVTTNIMG
                     RTICALGDAASMPVQSFVKHFRDEFAYHIEHKKCLVPLDVQRAGSGFFKATV"
     gene            complement(2283..2756)
                     /gene="nuoE"
                     /locus_tag="PLD53_07595"
     CDS             complement(2283..2756)
                     /gene="nuoE"
                     /locus_tag="PLD53_07595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRC60815.1"
                     /translation="MLTTESLQRIDREIAKYPADQKQSAVMAALAIAQEEQGRLSSEA
                     IEFVAHYLGMAPMAAYEVASFYSMYDLTPVGKYKLTVCTNLPCMLSGGVDAAEYLKNK
                     LGVGFNETTLDGKITLKEGECMGACGDAPVLLVNNRRMCSFMQPEQIDKLLAELE"
     gene            complement(2756..4009)
                     /locus_tag="PLD53_07600"
     CDS             complement(2756..4009)
                     /locus_tag="PLD53_07600"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRC60816.1"
                     /translation="MPDIQNYTLNFGPQHPAAHGVLRMVLELDGEVVVRADPHIGLLH
                     RATEKLAETKTWVQSVPYMDRLDYMSMMCNEHAYCMAIERLLEIEVPIRAQYIRVMYD
                     EITRILNHLLWIGAHGLDVGAMTMVLYAFREREDLVDCYEAVSGARMHAAYYRPGGVY
                     RDLPDSMPQYRESKWKSAAEVRKINEVRSGSLLDFLGDFVSRFPKILDEYETLLTDNR
                     IWKQRLVNVGIVSPERAMQLGFTGPMLRGSGIAWDLRKKQSYEVYDRLDFDIPVGVTG
                     DSYDRYLVRVEEMRQSNRIIKQCIDWLRVNPGPVITDNHKVAPPSRADMKSNMEELIH
                     HFKLCTEGIHVPEGQVYSAVEHPKGEFGIYAISDGANKPYRLKIRSPGYVHLSAMNEL
                     TRGHMIADVVTMVGTIDIVFGEIDR"
     gene            complement(4002..4607)
                     /locus_tag="PLD53_07605"
     CDS             complement(4002..4607)
                     /locus_tag="PLD53_07605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRC60817.1"
                     /translation="MSAKLDLLSQNLQQHCGNRITSQQIAFGEITIDVDVGDYLEVMT
                     TLRDEPELRFEELMDLCGVDYSTYKNSAWTGKRFAAVSHLVSIAHNWRVRVRCFAPDD
                     DFPSIPSITSIWNSVNWFEREAFDLFGIVFPGHNDLRRILTDYGFIGHPFRKDFPISG
                     YVEMRYDPDQQRVIYQPVTIEPREITPRIVREEGYGEVDNA"
     gene            complement(4636..5112)
                     /locus_tag="PLD53_07610"
     CDS             complement(4636..5112)
                     /locus_tag="PLD53_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRC60818.1"
                     /translation="MGIEGILQEGFITTTADKLINYMRTGSMWPMSFGLACCAIEMIH
                     AGCSRYDLDRFGIVFRPSPRQSDVMIVAGTLTNKMAPAMRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYAYSVVRGCDRIVPVDIYVPGCPPTAEALIYGLIQLQNKIRRTSTIAR"
     gene            complement(5117..5491)
                     /locus_tag="PLD53_07615"
     CDS             complement(5117..5491)
                     /locus_tag="PLD53_07615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRC60819.1"
                     /translation="MLENYFPILVFILIGIVFGFAPVITGMIVAPHRPDSEKLSPYEC
                     GFEAFEDARMKFDVRYYLIAILFILFDLEVAFLVPWASIFKEIVQTDSVKLFGFVEML
                     VFILILVVGYVYAWVKGALDWE"
     gene            complement(5550..5634)
                     /locus_tag="PLD53_07620"
     tRNA            complement(5550..5634)
                     /locus_tag="PLD53_07620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5598..5600),aa:Leu,seq:gag)
     gene            complement(5702..6073)
                     /gene="secG"
                     /locus_tag="PLD53_07625"
     CDS             complement(5702..6073)
                     /gene="secG"
                     /locus_tag="PLD53_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRC60820.1"
                     /translation="MEYLFSVVLVVHILAAIGVIGLVLVQHGKGADMGAAFGSGASGS
                     LFGATGSANFLSRTTAVLAAVFFVTSLSLAYIASNKPKTTGSVMQDAVRSQAVQEPVA
                     AGADKAGTPVESGSKTKDIPQ"
     gene            complement(6083..>6337)
                     /locus_tag="PLD53_07630"
     CDS             complement(6083..>6337)
                     /locus_tag="PLD53_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRC60821.1"
                     /translation="PVWAIGTGRTATSEQAQEVHAAIRAQIAAADAKVAEGLQILYGG
                     SVKPNNAVELFGMADIDGGLIGGASLVAADFLAICRAAAQ"
BASE COUNT         1432 a         1879 c         1687 g         1339 t
ORIGIN      
        1 gatttgctcg ccaccgtgct ggctggcgat atccttgagt ccatgtgaga cgtagtccag
       61 cgcgacattc cattcgactt cacgcaggac gccgtcgacg cgaatcatcg gcttctgcag
      121 gcgatcactg gagttcagcg cctcatagga aaagcgatcc ttgtcggata tccagcattc
      181 gttgatctct tcattctcgc gaggaagaac tcgcattacg cgatcgctct tgacctgtac
      241 cgtcaggttt gagcccaagc tgtcgtgtgg actgaccgac ttgcggcgcg acagctccca
      301 gctgcgggcc gagtaccgga atggcttcga agtcaatgcg ccaaccggac aaatgtcgat
      361 catgttgccg gacaattcgg aatcgaccgt gcggccaacg aaggtttgga tctccgaatg
      421 catgttgcgg ttggccatgc caagctccat tacgccggct atttcctggc caaaacggac
      481 acaacgcgtg caatggatac aacgtgccat ttcctgcatc gacaccagcg gacccacgtc
      541 cttgtggaaa acgacgcgct tggcttcctg ataacgtgag gcgctcccgc cataacccac
      601 tgccaaatcc tgcagctgac actcgccacc ctgatcacag atcgggcaat cgagcgggtg
      661 attgatcaac agaaactcca taacgccctt ctgggcattg acggcaagct ccgagtgggt
      721 aaacaccttc atgccactgg tgaccggcgt tgcgcaagcc ggcaagggct tcggagcttt
      781 ctcgacctgc accaggcaca tccggcaatt ggccgagatc gagagtttct tgtgatagca
      841 gaaatgcggg atgtaagcgc cgacgagctg ggcggcatcc atgatggtgc cccccgctgg
      901 tacttgaacc tgtttgccgt cgatttcaat atcaagcata tcgctacctt agaccgttgc
      961 cttgaagaag ccgcttcccg cgcgctgaac atccagcgga acaaggcact tcttgtgttc
     1021 aatgtggtac gcaaactcgt ctcgaaaatg cttgacgaag ctctgcactg gcatcgaggc
     1081 ggcatcaccg agcgcacaaa tggtgcggcc catgatgttg gtggtcacgc tgttgagcag
     1141 atcaaggtct tccgggcgcc cctgtccgct ttcaatgcgg tggacaatcc tgtagagcca
     1201 gttggtacct tcgcggcatg gcgtacactg cccgcacgat tcttcgtagt agaagtagga
     1261 aagtcgctca agcgccctca ccatgcaggt cgtctcatcc atgatgatga ccgcgccaga
     1321 accgagcatt gagccggctt tgctgatgga gtcgtaatcc atcgtgcact ccatcatgat
     1381 gtcgcccggc accacggggg atgatgaccc accggggatg cagcccttga gctggcgacc
     1441 gccacgcatg ccgccggcca tctcgagcag cgttgagaag ggcgtaccga gcggaatttc
     1501 ataatttccc ggcttgttga cgtggccgga aatcgagaac agtttggtgc cgccgttgtt
     1561 ggctttgccg gcttcgagat aagcctcgcc acccagctca agaatgaacg gaatggcgcc
     1621 aaacgtctcg gtgttgttga tcgtcgtcgg cttgccgtaa agaccgtacg atgcaggaaa
     1681 tggcggcttg aagcgcggct gccctttctt gccctcgatc gattcgagca gtgcggtttc
     1741 ttcgccgcag atgtaagcgc cgtaaccatg gtgcgcaaag agatcgaaat caaatcccga
     1801 gcccagtatg tcttttccca agaggccggc tgcacgggct tcgtccagcg catcttcgaa
     1861 acgcgtatag acttcccaga tttcaccgtg aatgtagttg taaccgcgtg aagctcccat
     1921 ggcataggcg gcaattgcca tgccttcaat caccgaatgc ggattgaaga gcaggatgtc
     1981 gcggtccttg aaggttcccg gttcaccctc gtccgaattg cagaccacgt atttgtcgcc
     2041 cggaaaggca cgcggcatga agctccactt caagccggtc gggaaaccgg caccgccccg
     2101 accgcgcagt acggatttct tgacttcgcc gatcacttgg tcctgaggca ttttctcggc
     2161 aagaattctt ttcagcgccg aatagccgcc acgctcgacg tagccatcca gtcgccaggc
     2221 acgatcgctt tcatagtcga ggccgacggt gagcatcggg ttgattgcac cgaggatcgc
     2281 cattattcga gctccgccag cagtttgtcg atttgctccg gctgcataaa gctgcacatg
     2341 cgacggttat taaccaacag cacgggcgca tcgccacagg cgcccataca ctcgccttcc
     2401 ttgagcgtga tcttcccatc gagcgtcgtt tcattgaaac caacgcccag cttgttcttc
     2461 aggtactcgg ccgcatcgac accaccggac aacatgcacg gcaggttggt gcagacagtc
     2521 agcttgtatt tgcctaccgg ggtcaggtca tacatgctgt agaagctggc cacttcgtag
     2581 gcagccatcg gcgccatgcc aagatagtgg gcaacaaact caatcgcttc gctcgacaac
     2641 cggccctgtt cttcctgggc gatcgccagc gctgccatca ccgccgactg cttctggtca
     2701 gccggatact tggcgatctc tcgatcaatt ctttgcaagg attctgtagt cagcatcagc
     2761 ggtcaatctc gccaaaaacg atgtctatcg tgccgaccat cgttactacg tcggcaatca
     2821 tgtggccacg cgtcagttca ttcattgccg acagatggac gtaacccggc gagcgtatct
     2881 tcagtcggta aggcttgttc gcgccatccg aaatcgcgta gatgccgaac tcacccttcg
     2941 ggtgctcaac ggccgagtag acctggcctt ccgggacatg aatgccctcg gtgcacaact
     3001 tgaagtggtg gatcaactcc tccatattcg acttcatgtc cgctcgcgac ggcggcgcca
     3061 ctttgtggtt atcggtaatt acgggacccg ggttgacacg cagccagtcg atgcattgct
     3121 tgattatgcg attcgactga cgcatttcct cgacgcgaac aaggtaccgg tcatagctgt
     3181 ctcccgtcac gccaaccgga atgtcaaaat cgagtcggtc ataaacctcg taggactgct
     3241 tcttgcgcag atcccaggcg atgcccgaac cacgaagcat cgggccggta aaacccaact
     3301 gcatggcgcg ctcgggagaa acaattccca cgttgaccaa acgctgcttc catatccggt
     3361 tgtcggtcag cagggtttcg tactcgtcga gtatttttgg aaaacggctg acgaaatccc
     3421 cgaggaagtc cagcagagag ccgctgcgca cttcattgat cttcctgact tctgccgcgc
     3481 tcttccactt cgactcgcga tactgcggca tgctgtccgg caggtcacga tagacgcccc
     3541 ccggcctgta atacgccgcg tgcatccggg cgccggacac ggcttcgtag cagtcgacca
     3601 gatcttcacg ctcacggaag gcgtaaagca ccatggtcat cgcgccgacg tcaaggccat
     3661 gcgcgccaat ccataacagg tggttcagaa ttcgcgtaat ttcgtcatac atcacacgga
     3721 tgtactgggc gcgaatcggc acctcgattt ccagcagccg ttcaatcgcc atgcagtagg
     3781 catgctcgtt gcacatcatc gacatgtagt cgagacgatc catgtacggc acggactgca
     3841 cccaggtctt ggtttccgcc agcttttcag tcgcgcgatg cagcaaccca atgtgcgggt
     3901 cggcacgcac gacgacttcg ccatcgagct ccagcaccat gcgcaatacg ccgtgtgcgg
     3961 ccggatgctg tggcccgaag ttgagcgtgt aattctggat atcaggcatt gtcgacttcc
     4021 ccgtaaccct cttccctgac aattctcggc gtgatctcac gcggctcgat cgtcacgggc
     4081 tgataaatga cgcgttgctg atcggggtcg tagcgcatct cgacataacc ggatatcgga
     4141 aagtccttgc ggaaaggatg cccgatgaat ccgtaatcag taagaatccg gcgcagatcg
     4201 ttgtgccctg ggaaaacgat gccaaacaga tcgaatgcct cgcgctcgaa ccagttgacc
     4261 gagttccaga tcgaagtaat cgaggggatc gaggggaaat catcgtccgg cgcgaaacag
     4321 cggacacgga cacgccagtt gtgcgcaatc gaaaccagat gacttaccgc agcgaatcgc
     4381 tttccggtcc acgcactatt cttgtaagtg gaatagtcga caccgcagag atccatcaac
     4441 tcctcgaaac gcaattcggg ttcgtcgcgc agtgtcgtca ttacttcaag ataatcgccc
     4501 acatcgacat cgatcgtgat ttcgccaaag gcgatctgct gggacgtgat gcggtttccg
     4561 caatgctgtt gcaggttttg gcttagcaga tcaagcttgg cggacattgg aaatactccg
     4621 ggcttctcgt ttggttcagc gggcgatggt gctggtgcgg cgaatcttgt tctgcaactg
     4681 gatcaggcca taaatcagcg cctcggcggt cggcggacaa ccgggaacgt agatatcgac
     4741 tggaacgatg cggtcacatc cacggacaac cgaatacgca tagtggtaat aaccgccgcc
     4801 attggcgcag gaacccatgg agatcaccca gcgcggctcg gccatctggt catagacctt
     4861 gcgcatggcc ggcgccatct tgttggtcag ggtgccggca acaatcatca catccgactg
     4921 tcgcggactg ggtcgaaaca cgatgccgaa acggtccaga tcgtagcgcg agcaaccggc
     4981 atgaatcatc tcgattgcgc aacaggccag accgaagctc atcggccaca tcgaaccggt
     5041 tcgcatgtag ttgatcagct tgtcagctgt cgtcgtgatg aagccttcct ggagaatgcc
     5101 ttcaataccc atttgctcac tcccaatcca gcgcaccctt gacccaggca tagacgtagc
     5161 ccacgacaag aatgaggatg aagaccagca tctcgacaaa accgaacagc ttgaccgaat
     5221 ccgtctggac gatttccttg aagatcgacg cccatggcac aaggaacgcc acttcgaggt
     5281 caaacagaat gaacaggatg gcgatcaggt aataccgcac gtcgaacttc atacgtgcgt
     5341 cctcgaaagc ctcgaagccg cattcgtagg gcgaaagttt ttcgctatca gggcgatggg
     5401 gcgctacgat catgccggtg atgaccggcg cgaaaccaaa aacgattcca atcaggatga
     5461 aaacaagtat cgggaaatag ttttccagca tgatccgcca cactatcaat tacatttcaa
     5521 ccagagcttc ccaatcgggt aactccactt ggtgccgacg atgagactcg aactcatacg
     5581 acctaagtca ctaccccctc aagatagcgt gtctaccaat ttcaccacgt cggcactgct
     5641 acagtcaggc ggcgatgtgc ctgaacgagg gaaagcacac cgccgctttc atacagtcaa
     5701 tctactgcgg aatatctttg gtcttggagc ctgactccac gggagttcca gccttgtcgg
     5761 caccggccgc aaccggctcc tgaacagcct gcgatcttac cgcatcctgc atcacactgc
     5821 cagtcgtttt tggtttattg gaggcgatat aagccaacga aaggctggtc acaaaaaaaa
     5881 cagcggctag caccgctgtt gtccggctca ggaagttggc agaccccgtc gcaccaaaca
     5941 ggctgcccga agcgccacta ccgaaagctg cgcccatgtc agcgcccttg ccgtgctgaa
     6001 ccagcacgag accgatgaca ccgatcgccg ccagaatgtg aacgaccaaa accacagaaa
     6061 acagatattc cattttgtct ctttattgag cagctgcgcg gcaaatcgcc aggaaatcag
     6121 cagcaaccaa agacgcgccc ccaatcaggc caccatcaat atcggccatg ccgaaaagct
     6181 cgactgcgtt gttgggcttt acactgccgc cgtaaaggat ttgtagcccc tcggccacct
     6241 tggcatcagc agcggcaatc tgcgcgcgaa tggctgcgtg cacttcctgc gcctgctcgg
     6301 aagtcgccgt gcggccggta ccgatggccc ataccgg
//
LOCUS       DAORPM010000136         6277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3328_length_6277_cov_12.710543, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000136 DAORPM010000000
VERSION     DAORPM010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_6277_cov_12.710543"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PLD53_07635"
     CDS             <1..689
                     /locus_tag="PLD53_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRC60822.1"
                     /translation="VQCNEMNQLALSGQLDPCMSRAFTYEELPVAHQLMADNKHPHGN
                     MSVLIGAPGFGLGASGKPAVTTVHPTLPKGDVHTTPHPYPMSEPLPSIEPAEALTIAD
                     DGTKVRDLMHRGIISCTPDDTVGAVAKIMVDKEIHAVVVMEQGKATGVVSQTDMVLAR
                     QGRTPDQARSMKASEVMTPGCATCDADMLLSDAVSLMTGRRMHRLVVTEKDQPVGVIS
                     MTDVVRKIIG"
     gene            complement(822..1349)
                     /locus_tag="PLD53_07640"
     CDS             complement(822..1349)
                     /locus_tag="PLD53_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC60823.1"
                     /translation="MIVTEVASMIIHDSQRGTGFLLTDSARLLRKLIDRRLQPLGLSR
                     AQWSILAILSNQEGLSQSQISSKLEIEKSTAGRLIDQVEKSGWIERRPIVGDRRVWGI
                     YLTNQARQLLAQVETVILHSRTEMLNGLSAEQQQSLSEMLQTVKSNLSTALECDQRPI
                     PAVAETAPYDGNHAN"
     gene            1861..2523
                     /gene="can"
                     /locus_tag="PLD53_07645"
     CDS             1861..2523
                     /gene="can"
                     /locus_tag="PLD53_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HRC60824.1"
                     /translation="MKYLPELFAANTRWAAETRAADPNFFSGLANLQRPEYLWIGCSD
                     SRVPANQITGLKPGEVFVHRNVANAVVHTDLNCLSVMQYAIDVLKVRHIIVCGHYGCG
                     GVRAALEGPSLGLIDNWLRHIQDVRDRHMEFLAALPDDTARWCALCELNVIEQVRNVA
                     RTTLVGDAWQRGQPLMLHGWIYGLEDGRLQDLQSSLDNAGEGDTVVAQAVATTRSRYS
                     AS"
     gene            2525..3205
                     /locus_tag="PLD53_07650"
     CDS             2525..3205
                     /locus_tag="PLD53_07650"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC60825.1"
                     /translation="MPTRIYLVRHGATDLTAEDRFAGSSDVALSDEGRKQVACLAERL
                     KGQQLDAVYASPLQRTMETARTLAGPHGLQPIAEPGLREIDYGHWEGLRRSEVESAFQ
                     AEYAIWQEDPFTIAPLDGESGVNVLNRALPVMRGIVERHRRRSVLVVSHKGTNRLLIS
                     SLLGFDMRGYRDRLDQSPAALNILDFMSEVRARLRLFNDVSHYENIPERILTERLSSW
                     WGNPQGTK"
     gene            3399..4376
                     /locus_tag="PLD53_07655"
     CDS             3399..4376
                     /locus_tag="PLD53_07655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HRC60826.1"
                     /translation="MRAVVCHEFTGPAALRLEDIPEPRPGTGQVRILVEACGVNFADS
                     LISRGQYQKQPQPPFSPGFEVSGKVLELGAGVEELKIGDRVIAMTPHGGYAEQVVADS
                     NRCVQMPATMSWQDGAAFPVVFGTSHIALWHRARLQAGETLVVHGASGGVGLTAVAIG
                     KRLGATVIATANGPGKLEVAREHGADHLIDSGQEDVRLRIKELTDGRGANVVYDPVGG
                     ELFTASLRSIAFEGRILLIGFASGTVPQIPANHLLVKNVDVIGVNWPAYAELHPEVMT
                     ESFRTLIQWYLDGAIKPHISASYPLEQAVEALNQVVSRKSTGKVVIVPK"
     gene            4522..6171
                     /locus_tag="PLD53_07660"
                     /pseudo
     CDS             4522..6171
                     /locus_tag="PLD53_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
BASE COUNT         1281 a         1773 c         1917 g         1306 t
ORIGIN      
        1 ccgtgcagtg taacgagatg aaccagttgg cattgagcgg tcagcttgat ccctgtatgt
       61 cgcgggcgtt cacctatgaa gaattaccag ttgcgcatca gttgatggct gacaacaaac
      121 atccacatgg caacatgtcg gtgctgattg gcgcccccgg gtttggtctc ggtgcgagcg
      181 gcaagccggc tgtgacgact gtgcatccga ccctgcccaa gggcgatgtg catactacgc
      241 cgcatccgta cccgatgtcc gagccgttgc ccagtattga gcctgccgaa gccttgacca
      301 ttgccgacga cggcaccaag gttcgcgact tgatgcatcg cggcattatt tcctgtacgc
      361 cggatgatac ggttggcgct gtggccaaga tcatggtgga caaggagatt cacgcggtag
      421 tggtcatgga gcagggcaag gccaccggtg tcgtatcgca gaccgatatg gtgctggcgc
      481 gtcagggccg gacgccggat caggcgcggt cgatgaaggc cagcgaagtc atgactcccg
      541 gctgcgcgac ttgcgatgcg gatatgctgc tcagcgatgc agtcagtctg atgactggcc
      601 gtcgcatgca tcgcctggta gtaaccgaga aggatcagcc ggtcggcgtg atttccatga
      661 ccgacgtggt acgcaagatc atcggctagt agtgcggttt tgccgcggag cccgatacag
      721 gcattctgta ttgggtcgtg gtgaagtagt tatagggcgc gggctgtcag accgaaggaa
      781 gatttcgggc aggcagcccg cgttgttttt gaagcggcga atcagttagc gtggtttccg
      841 tcgtatggcg ccgtctccgc aacggctgga attggtcgct ggtcgcattc aagtgcagtt
      901 gacaggttgg acttcactgt ttgcagcatc tcggacaggc tctgttgctg ttcggctgac
      961 aggccgttca gcatttccgt gcgactgtgg agaatgactg tttcgacctg cgcaagcagt
     1021 tgtcgcgcct ggttggtcag ataaataccc cacacacgac ggtccccgac aatcggacgt
     1081 cgttcgatcc atccgctttt ttcgacctga tcgatcaacc gacctgcagt ggacttctcg
     1141 atttccagtt tgctggatat ctgcgactgg gaaagacctt cttggttgga gaggatcgcc
     1201 aggattgacc attgcgctcg tgacaagcct agcggctgca atcgacggtc gatcagtttt
     1261 cgcagcaatc gggcagagtc agtgaggaga aaacccgtgc ctcgctgcga atcgtggatg
     1321 atcatggatg cgacctcagt gacgatcaag tgatggcatt cgtctgccgt cgattggcgc
     1381 ggcttttagc gcaggcatta ccggtagact aacccagaac tctcgtcagg cggcgagcgt
     1441 gcccagaccg gcaaggtgga tcatagaagc ttccgcaaga cgattgagga ttgagggcgc
     1501 gtctcgtcgg tcgcagaaag ccagaaatct gtagtaagct tgcttattgt atgttgacta
     1561 ctggttagtt gaaaaggtaa aattttgcgt cagatgaagg agataccgag cgcgttgctt
     1621 catcgaagac cggttcgacg ttctacccgc cggtttctgt cgcgattgcc atggccgatg
     1681 cgtgattgaa ggcggggaag cgcctgatac ccggtgtttc ctgtctgaac cggcgcccgg
     1741 acgatcatcg aaatctaccg gacgccgagt agaaaacgag gtttgaccac tcggacaagg
     1801 tagtcgagaa gctgaccgag cttgccaagc gtttcccttt aatcatggaa tgacttcaac
     1861 atgaaatacc tgccggaact gtttgccgcc aacacccgtt gggctgcgga aacacgagcc
     1921 gccgatccga atttcttctc gggactggcc aacctgcaac gtccggaata tctgtggatc
     1981 ggttgttccg atagccgggt accggccaat cagatcacag gcctgaaacc gggcgaagtc
     2041 ttcgtgcatc gcaacgtcgc caacgcggtc gtgcataccg atctcaactg cctgtcggtg
     2101 atgcaatacg cgattgatgt actcaaggtc cggcacatca ttgtctgcgg tcattacggc
     2161 tgcggcggtg tcagggcagc tctggaaggg ccttcgttgg gactgatcga caactggttg
     2221 cgccatattc aggacgtgcg cgatcgacat atggaattcc tcgccgcgct gccggacgat
     2281 actgcccgct ggtgtgccct gtgcgaactg aacgtcatcg agcaggtgcg caatgttgcc
     2341 cgcacaacgc tggtcggcga cgcatggcag cgcggccagc cactcatgct acatggatgg
     2401 atctatggtc tggaggacgg acgtctgcaa gacctgcagt cctcgttgga caatgccggc
     2461 gaaggggata cggtcgtcgc ccaggcggtg gctacgaccc gctcgcgtta ttcagccagc
     2521 tgatatgcca acgagaatct acctggtgcg tcatggtgcc accgatctca ctgcagaaga
     2581 ccggttcgcg ggttcgtcgg atgtggctct gtccgacgag ggccgcaaac aggtggcgtg
     2641 cctcgcggag cgcctgaagg ggcagcaact ggacgccgtc tatgccagtc cgctacagcg
     2701 caccatggaa accgcgcgca ctcttgcggg tccgcacggc ctgcaaccga tcgccgaacc
     2761 gggattgcgc gaaatcgact acggtcactg ggagggactc cgccgatccg aggtcgagag
     2821 cgcgttccag gccgagtacg ccatctggca ggaggatccg ttcaccatag cgccattgga
     2881 tggcgaatcc ggggtcaatg tgctcaatcg tgcattaccc gtgatgcggg ggatcgtcga
     2941 acgtcatcgc cgccgcagtg tactcgtcgt atcgcacaag ggaaccaatc gtttgctgat
     3001 cagcagcctg ctgggtttcg acatgcgggg ctaccgcgac cggctggacc agagcccggc
     3061 ggcattgaac atcctcgact tcatgagcga ggtgcgcgcc cgcttgcgct tgttcaacga
     3121 tgtttcacat tacgagaata taccggagcg tatcctgact gaacgcttgt caagctggtg
     3181 gggaaatccc caagggacca agtaaacgta gccccggtct cgactcagac cgcgattgga
     3241 atggaattca ctagcccagt catccacggt tggccatgtc gcagtccaag gatggcgcta
     3301 gcgcctgccc tcctgttcta gaacggatac actagtcgtc tgcctcgtcc cgcaggacga
     3361 agtacggtct tttgttcaac gatggaggat tcacaaccat gcgagccgtc gtctgccatg
     3421 aattcaccgg gccagctgcc ctgcggctgg aggatatccc ggagccgcgg cccggtaccg
     3481 gccaagtgcg tatccttgtg gaggcctgtg gcgtcaattt cgccgatagc ctgatctccc
     3541 gcggacaata tcagaagcag ccacaaccgc cgttcagccc cggctttgaa gtctctggca
     3601 aggtgctgga actgggggca ggagtggaag agctgaaaat cggggatcga gtgatcgcca
     3661 tgacgccgca cggtggctat gccgagcagg ttgtcgcgga cagcaaccgc tgcgtgcaaa
     3721 tgccggcgac aatgtcctgg caggatggcg ccgctttccc agtggtgttt ggtacgtccc
     3781 atatcgcctt gtggcatcgg gcacgcttgc aggctggaga gacgctggtg gtgcatggtg
     3841 cctcaggcgg cgttggactg accgccgtag ccattggcaa gcgcttgggc gcaacggtca
     3901 tcgccacagc taacgggccg ggaaagcttg aagtcgcgcg ggaacacgga gcggaccatc
     3961 tgatcgattc cgggcaggag gacgtgcgct tgcggattaa ggaactcacc gatggccgcg
     4021 gtgcgaacgt ggtttacgat ccggtcggtg gtgagttgtt caccgcgtcg ctgcgcagca
     4081 tcgcctttga aggtcgcatt ctgttgatcg gtttcgcgag tggcaccgtg ccgcagattc
     4141 ccgccaacca tctgctggtc aagaatgtgg atgtgatcgg cgttaactgg ccggcctatg
     4201 cggaactgca tcccgaggtg atgaccgaga gcttccggac gctgattcaa tggtatctgg
     4261 acggtgcgat caagccgcac atttcggcaa gttatccact ggaacaggcg gtagaggcgc
     4321 tcaatcaggt tgtgagccgc aagtccaccg gtaaggtcgt catcgttccg aagtagggct
     4381 gttgagcagt agaggagtca ttcgttttcg gctctttttc cgctgcatca gggccgacgc
     4441 cgccgatgcc gacttcctgc attggacgta atatcttaga atcgcttata tacccgacaa
     4501 cagactacgg acgcgaggag caagatgcaa cgagaatcca tggaatacga tgtcgttatc
     4561 gtcggtggcg gcccggccgg gctgtctgcc gcgatccgcc tcaagcaact tgccgctgaa
     4621 aaggaaggtg agatcaacgt ttgcctgatc gagaaagcgg ctgagatcgg ggggcatatt
     4681 ctttctggag cagttctcga tccgcgagcg ctggccgagc tctttcccga ttgggagtcc
     4741 ctgggcgcac cgctcaaggc gccggtcagc gaagaccgct ttctgatgct caaggaaggc
     4801 ggcggctttc agataccgaa ctggatgtta cccggctgct ttcacaatgc gggcaaccac
     4861 gtgatcagtc tgggcaatct ctgccgctgg ctcggcgagc aggccgaagc gctcggcgtc
     4921 gaaatctatc ccggttttgc cggtgccgag gtgctctaca acgaggaagg ctcggtcaag
     4981 ggcgtggcca ccggtgacat gggtgtgctc aaggatggca gccagggccc ggcctaccag
     5041 cccggcatgg aactgctcgc caaatacacc ttgtttgccg aaggttgtcg cggccacttg
     5101 ggcaagcaac tcgaagagaa attcaatctg cgcgagaacg ccgaagcgca ggtttacggc
     5161 atcggtctca aggaactctg ggaagtcaaa ccggagaagc ataaatccgg gctggtcatt
     5221 cataccggcg gttggccgct tgacccgcag acgtacggcg gcggcttcat gtaccacatg
     5281 gaagacaatc aggtatcggt cggcttcgtc gccggactcg gctacagcaa cccctatttt
     5341 tcgccctatg aggaattcca gcgtctgaaa acccatccgg cgctgcgcac gtttctcgaa
     5401 ggtggcaagc gcatcgccta tggtgcccgt gccctcgccg ccggcggtct gcaatccctg
     5461 cctaaactga tctttcccgg cggtgcgctg attggcgacg atgccggctt cctcaacgcc
     5521 gctcgcatca agggctcgca ttgcgccata aagacgggtt cattggcagc ggaggcctgc
     5581 ttcgacgccg ttgcggccgg ccgcagcagt gatgaactgg acgcgtatcc cgaagccttc
     5641 cgcagcagct ggctgtttga cgagctgcac aaggcacgta acttcaaacc ctggatggca
     5701 agaggcttgc atcagggcag tctgctattc ggcatcgacc agatcgtgtt ccgtggcaag
     5761 gcgccgtgga cattgcacca cagtgccgca gaccatacca agctgaaaaa agcggccgat
     5821 tgccagccca tcgcctaccc caaacccgat ggcgtcatta ccttcgaccg actgtcgtcg
     5881 gtctatctct cgaataccaa ccatgaggaa gaccaactct gccatctgac gctcaaggat
     5941 gccgcgctgc cgatcacggt caacctcgcg ctctatgacg cgcccgaaca gcgttactgc
     6001 cccgccgggg tgtacgaaat cgtacgcgat gatgccggcg acaatccccg cttgcagatc
     6061 aatgcgcaga actgtgtgca ttgcaagacc tgcgatatca aggaccccac gcagaacatc
     6121 acgtgggtgg taccacaagg cggcgagggg ccgaattatc cgaatatgta gctttggttc
     6181 ctcaggccgg tatcagcgtt gactttcagt cttcgctgac gccgtcccct gctcacccca
     6241 gcgcgttggt caatgcggga gccagctcga acagatc
//
LOCUS       DAORPM010000137         6271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3331_length_6271_cov_8.046654, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000137 DAORPM010000000
VERSION     DAORPM010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3331_length_6271_cov_8.046654"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..958
                     /gene="nifM"
                     /locus_tag="PLD53_07665"
     CDS             128..958
                     /gene="nifM"
                     /locus_tag="PLD53_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifM"
                     /protein_id="HRC60827.1"
                     /translation="MMQHGYLELKLSWQLFEKTPDTLTEDERRRLLVVAEKQQAIEQC
                     ILASAEAANVVVVRRTLSTRLEEIHKRYASEDEFTQDLERVGIGEVELEKAVERDLRV
                     DAVLEKVASSAATVSAVDAEIFYRLHPEAFDRPESRRLRHILLTFSNAKKKARAFAQL
                     EACRYNVKGAERFGEMALRHSHCPTATDGGKLGVVKRGQLYPELEPAAFSLAEGEISR
                     VLESPIGLHILHCDEILPGGIMPFVEVRQRIIDHLFEKRRHKKQRDWIKRRQLRRAKS
                     "
     gene            complement(1029..2261)
                     /gene="nifS"
                     /locus_tag="PLD53_07670"
     CDS             complement(1029..2261)
                     /gene="nifS"
                     /locus_tag="PLD53_07670"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HRC60828.1"
                     /translation="MDAPIYLDNNATTRVDPVVVEAMLPFFTEHFGNASSIHAFGSEV
                     GKALKKARSQVQELLGAEHDSEIIFTSCGTESDSTAILSALKAQPDCNTVITTTVEHP
                     AVLNQCEWLEKDGYIVHKLRVDKKGRVDLDEYKSLLHERVAVVSVMWANNETGTLFPV
                     LEMAELAAAKGIMFHTDAVQAVGKIPIDLKSGKIDMLSLSGHKLHAPKGIGVLYLRRG
                     CRFRPLLRGGHQERGRRAGTENSASIVGLGKACELATQHMEEENTRVKGLRDKLEAGI
                     LSQVSHCFATGDTHYRLPNTSNIAFEYIEGEAILLLMNKLGIAGSSGSACTSGSLEPS
                     HVMRAMGIPYTAAHGTIRFSLSRFNTEDEIDRVIAGVPPIVAQLRKLSPYWSDSGDSA
                     GKSGPAVKPEGAFAPTYA"
     gene            complement(2313..3221)
                     /gene="nifU"
                     /locus_tag="PLD53_07675"
     CDS             complement(2313..3221)
                     /gene="nifU"
                     /locus_tag="PLD53_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HRC60829.1"
                     /translation="MWEYSDKVREHFFNPRNSGPLEDANGIGDVGSIQCGDALRLMLK
                     VEPETEIIRDAHFQTFGCGSAIASSSALTEIVKGMTLDEALKVSNQNIADYLDGLPPE
                     KMHCSVMGREALQAAIANYRGEVWSDDHEEGALICKCFAVDAVMIEDVVKANKLNTVQ
                     EVTFYTKAGGGCAACHEGIEEILARVMAESAGPGEVSPPSAAILPPVAKPEVSKPASV
                     KLTLVQKIRKVEEVLDSVRPMLLRDNGDVELADIQGKKIYVHLKGACSGCMMEAATLG
                     GIQQKMIETLGELVQVLPSSHMPVEA"
     gene            complement(3236..3559)
                     /locus_tag="PLD53_07680"
     CDS             complement(3236..3559)
                     /locus_tag="PLD53_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRC60830.1"
                     /translation="MINMTPAAVKAVQRFIRGSETPVAGLRLVISGGGCSGLQYGMKL
                     EEGKAEDDWELEVDGVKLLVDPMTFPMIDNVTIDFIDSLTHTGFKFDNPNASAQCSCG
                     SSFKL"
     gene            complement(3700..3972)
                     /gene="fdxB"
                     /locus_tag="PLD53_07685"
     CDS             complement(3700..3972)
                     /gene="fdxB"
                     /locus_tag="PLD53_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HRC60831.1"
                     /translation="MITGLTKGGTEWTPEFVTDLDQAKCIGCGRCYKVCPRDVLDLVE
                     RELDDDDEDNDDDNMMVMALANAADCIGCGACGRVCPKGCYTYAAA"
     gene            complement(3969..4187)
                     /locus_tag="PLD53_07690"
     CDS             complement(3969..4187)
                     /locus_tag="PLD53_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HRC60832.1"
                     /translation="MTDEELKDLDKEVKKLKRISSEWAAQMHDLVEDRLPAAYQEIPA
                     LSQSTFAACQAWAEASARLTEALKGDSA"
     gene            complement(4202..4651)
                     /locus_tag="PLD53_07695"
     CDS             complement(4202..4651)
                     /locus_tag="PLD53_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HRC60833.1"
                     /translation="MSVDPIFETDFVKEMARQMRALDTYGSYAGWSVEKVLDPYVMTK
                     ERKANVPLIGDPDEETISRVKAFYNAIAVLIEKECKLLAVPLVHLTHEGFGRALITVG
                     KLVAVDRTLRDVHRFGFASLSKMKDEADKILSVAIERIGQYPAVAGL"
     gene            complement(4629..5111)
                     /locus_tag="PLD53_07700"
     CDS             complement(4629..5111)
                     /locus_tag="PLD53_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HRC60834.1"
                     /translation="MNAGIIEHRGTVQRVEEGKAVVAMETKGCGGCGHESACGIGQLA
                     NGRAASLISLPVHSDIKVGDQVNIALRESCLTLFALFGYLFPVFAMMLGAWFGVMHDG
                     SDGAAALGAMAGFIVALLIVRLVIRLLPGLAPAPQVFVISDHPTLSQQEYHHECRSHL
                     "
     gene            complement(5108..5611)
                     /locus_tag="PLD53_07705"
     CDS             complement(5108..5611)
                     /locus_tag="PLD53_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRC60835.1"
                     /translation="MANAKGNPVSTRRLQLVWSIKREPVAVIRVAFATTDRVRVNQHF
                     GAAEGFAIYDVVPDKATLVGVAEFADELMDGNEGKLSAKVEFLDGCNAVYVTAIGASA
                     IKQLTARGIQPIRINEVDHVDVLLHDIALAMSEGGVSWIDRAIAAQAKSRSDDRFAAM
                     EEEGWEG"
     gene            complement(5550..>6271)
                     /locus_tag="PLD53_07710"
     CDS             complement(5550..>6271)
                     /locus_tag="PLD53_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HRC60836.1"
                     /translation="TLVIGPSLDKAAAILKTRTGVPDYRFAGLMGLDDCDAFTQALAD
                     ISGKPVPAKLERHRAQLQDAMVDCHFALGFARIALAADPDLLGMLVRFLTGMGAEMVS
                     IVSPAKAESLASLPIPDVIVGDLEDLEKQASASHAQLLIANSHAAETARRLGLPLLRA
                     GFPQYDHVGGYARTWVGYRGTRQALFDLANLMQSQHHELEPYRSLYWAESRDDVGVEQ
                     HLGQRQGQPGVDAPAATGLVH"
BASE COUNT         1283 a         1851 c         1848 g         1289 t
ORIGIN      
        1 aggtcattcg cgacgcgccg ggcgacgttg tctatcacat ccacttcgac tgtctgccag
       61 gacgcgtact gcaaatcccc gaggatgcgc tggaagccgc ggagtgtggc ggatacagag
      121 gcgcaatatg atgcaacacg gctatcttga gctgaagctc tcctggcaac tgttcgaaaa
      181 gacacctgac acgctcaccg aagatgagcg tcgccgccta cttgtcgtcg cggaaaagca
      241 acaggcgatc gaacagtgca ttctggccag cgccgaagcc gccaatgtcg ttgtcgtgcg
      301 ccgcacattg tccacgcggc tggaggaaat ccacaagcgg tacgcgagcg aggacgaatt
      361 cactcaggat ctggagcgcg tcggcatcgg ggaagtcgag ctggaaaagg ctgtcgagcg
      421 cgatctgcgt gtcgacgcgg tgctcgaaaa ggtggccagc tcggcagcga cggtcagcgc
      481 cgtcgacgcc gaaatcttct accgcctgca cccggaagcc tttgaccgcc ccgagtcgcg
      541 acgactgcga catattctgc tgaccttcag caatgccaaa aagaaagcca gggccttcgc
      601 gcaactcgaa gcgtgtcgat ataacgtaaa gggtgctgaa agattcggcg agatggcgct
      661 acgccattcg cattgcccga cggcgaccga tggcggcaaa ctcggcgtgg tcaaacgcgg
      721 gcaactctac cctgaactgg agcctgccgc cttttcactt gccgaaggtg aaatcagcag
      781 ggttcttgag tcgcccatcg gtctgcacat cctgcattgc gacgagattc tgccgggcgg
      841 catcatgccc tttgtcgaag taaggcaacg aataatcgac catctgttcg agaaacgccg
      901 gcacaagaag caacgagact ggatcaagcg tcgccaattg cggcgggcca agtcctgatc
      961 gccgataaga caacggacaa cgagaacaac gcgggcaaag caagtctatg gactgctcta
     1021 aagactattc aggcatacgt cggagcaaat gcgccttcag gtttgacggc ggggccgctc
     1081 ttgcccgcgc tgtcaccact gtcgctccag taaggtgaga gcttgcgcaa ctgagcgacg
     1141 ataggcggta cgccggcgat cacgcggtca atttcgtctt ccgtgttgaa acgcgacagc
     1201 gagaagcgga tcgtgccgtg cgcagccgtg tagggtatcc ccatggcccg catcacatgc
     1261 gagggctcga gtgagccaga ggtgcaggcg gagcccgaac tgccggcaat ccccagcttg
     1321 ttcatcaaca agagtatggc ctcgccctcg atgtattcaa aggcgatgtt gctggtgttg
     1381 ggcaggcggt aatgcgtatc gccggtcgca aagcagtggc tgacctgact gaggattccc
     1441 gcttcgagtt tgtcgcgcag ccctttgacg cgggtgtttt cctcttccat gtgttgcgta
     1501 gccagttcgc aggctttgcc gaggccgacg atggacgccg agttttcggt gccggcgcgg
     1561 cggccgcgtt cctggtggcc gccgcgcaac aacgggcgaa agcggcagcc gcggcgcaga
     1621 tagagaacgc cgataccctt gggcgcatgc agtttgtgcc cggaaaggga gagcatgtcg
     1681 attttgccgc tcttcaggtc gatcggtatc tttcctaccg cctgcacggc atcggtgtgg
     1741 aacatgatcc ccttcgccgc ggccaattcg gccatttcca atacgggaaa cagggttccc
     1801 gtttcgttgt tggcccacat caccgagacg acggctaccc gttcatgcag caatgatttg
     1861 tattcgtcaa ggtcgacccg ccccttcttg tccacgcgta atttgtggac gatgtaaccg
     1921 tccttctcca gccactcgca ttggttgagg acggccggat gttcaactgt ggtggtgatg
     1981 accgtgttgc agtcgggctg ggctttgaga gcggaaagaa tggcggtgga gtcggattcg
     2041 gtaccgcacg aggtgaagat gatttccgaa tcgtgttcgg cgccgagcag ctcctgcacc
     2101 tggctgcgcg ccttcttgag cgccttgccc acttcgctgc caaaggcgtg gatcgacgat
     2161 gcattgccga aatgctccgt aaagaatgga agcatggctt ccaccacgac gggatcaacg
     2221 cgtgtggtgg cgttgttgtc caggtagatc ggagcatcca tggcaaattc ttcctgttgg
     2281 tggttggggc aactggctgg aaaatccaga atctaggctt cgaccggcat atgcgatgag
     2341 ggcaaaacct gcaccagctc gccgagcgtt tcgatcatct tttgctggat gccgccgagc
     2401 gtggcggcct ccatcatgca acccgagcag gcgcctttca gatgcacgta gatcttcttg
     2461 ccctggatgt cggccagttc aacatcaccg ttatcgcgca ggagcatggg gcgcacgctg
     2521 tcgagcactt cctcgacctt cctgatcttc tgcaccagcg ttagcttgac cgatgctggc
     2581 ttcgaaactt ccggcttcgc tacaggtggg aggatcgcgg cggacggggg cgaaacctct
     2641 ccggggccgg cgctttcggc catgaccctg gccaatattt cttcgatccc ttcgtggcag
     2701 gcagcgcagc cgccaccggc cttcgtatag aaggtcactt cctggacggt attcagcttg
     2761 ttcgccttga ccacgtcttc gatcatcacg gcatcgacgg cgaagcactt gcagatcaac
     2821 gcaccctctt cgtggtcatc gctccacact tcaccccggt agtttgcgat ggccgcctgc
     2881 agcgcttcgc ggcccatcac cgagcagtgc atcttttcgg gaggcagacc gtcgagatag
     2941 tcggcaatgt tctggttgga gaccttgagc gcctcatcaa gcgtcattcc cttgacgatt
     3001 tcagtcagcg ccgaggagga agcgatggct gagccgcaac cgaaagtctg gaaatgcgcg
     3061 tccctgatga tctcggtttc cggctcgacc ttgagcatca ggcgcagcgc atcgccgcac
     3121 tggatggaac cgacatcgcc aattccattc gcatcctcca gcgggcccga attgcgcggg
     3181 ttgaagaaat gttcccgaac cttgtcagag tattcccaca tgctatgagt gctccctaaa
     3241 gcttgaatga cgagccgcag gagcactgcg cgctggcgtt cgggttgtcg aacttgaatc
     3301 cggtgtgcgt cagcgagtcg atgaaatcga tcgtaacgtt gtcgatcatc ggaaaggtca
     3361 tcgggtcgac cagcaacttg accccgtcga cttcaagctc ccagtcgtct tcggccttgc
     3421 cttcttcgag cttcatgccg tattgcaggc cggagcagcc accacccgaa atcaccaggc
     3481 gcaagccggc aaccggcgtc tcggaaccgc gaatgaagcg ttgtacggct tttacggcag
     3541 cgggcgtcat atttatcatg tcggttctcc cagagtccat gtaatgcatt agtgcaaacc
     3601 ttgtgccaac tgcgcatttt ggctaagccc ttgtattgac taacttgtct cgacgccgag
     3661 ggatgtcgtg tttgcgacaa tccgccataa gcttcagagt caggcggctg cgtaggtata
     3721 gcagcccttc ggacagacgc gaccgcaggc accgcaaccg atacagtccg ccgcgttggc
     3781 cagcgccatg accatcatgt tgtcgtcatc gttgtcttcg tcgtcgtcat caagctcgcg
     3841 ttcgaccaga tcgaggacat cgcgcgggca aaccttgtaa cagcggccac agccgatgca
     3901 cttggcctga tcgagatcgg tgacgaattc cggcgtccat tcggtgccgc ccttggtgag
     3961 tccggtaatc atgctgagtc tcctttaagt gcttctgtga gacgggcgct ggcctcggcc
     4021 caggcctggc aggcggcaaa agtcgattgc gaaagtgccg ggatctcctg ataagccgct
     4081 ggcaggcggt cttcgacaag atcgtgcatt tgcgccgccc attcggaaga gatgcgcttc
     4141 aatttcttga cctccttgtc gaggtctttc agttcttcgt ccgtcatgct ttctcccgac
     4201 cttatagacc ggcaacggcc ggatactgcc cgatgcgttc aatggccacg ctcagaatct
     4261 tgtcggcctc gtctttcatc ttggacaggc tggcaaagcc gaagcgatgg acgtcgcgca
     4321 gggtacgatc gacagcgaca agcttgccga ccgtgatcag cgcacggcca aatccctcgt
     4381 gtgtcagatg aaccaatggc acggcgagca acttgcactc cttctcgata agaacggcga
     4441 tggcgttgta aaaggccttg acgcgggaaa tggtttcttc gtccgggtcg ccgatcaacg
     4501 gcacattggc cttgcgctcc ttggtcatga cgtaggggtc gaggactttt tcgacgctcc
     4561 agccggcata ggagccatag gtatccaggg cgcgcatctg gcgggccatt tccttgacga
     4621 aatcggtttc aaagatggga tcgacactca tggtggtact cctgttggga cagtgtggga
     4681 tgatcggaaa tgacaaagac ttgcggcgca ggcgccaggc ccggaagcaa tcgaatgacc
     4741 aaacgcacga taagcagcgc gacgatgaaa ccggccatcg cgcccagtgc ggctgcaccg
     4801 tcgctgccat catgcataac gccaaaccag gcgccgagca tcatggcaaa taccggaaag
     4861 agataaccga atagcgcgaa gagggtcagg cagctttcgc gcagcgcgat attgacctgg
     4921 tcgccgacct tgatgtcaga gtgaaccggc agagagatca aactggcggc acgcccgttt
     4981 gccaactggc caataccgca ggcgctctca tggccgcagc cgccgcagcc tttggtttcc
     5041 atggcgacaa cggccttgcc ttcctcgact cgctgcacgg tgccgcgatg ctcgatgatt
     5101 ccggcattca tccttcccac ccttcttctt ccatggcggc gaagcgatca tcgcttctgg
     5161 atttggcctg ggcggcgatg gcgcgatcga tccaggagac accaccttca ctcatggcga
     5221 gggcgatatc gtgcaaaagc acatcgacgt gatcgacctc gttgatccgg atcggctgga
     5281 ttcccctggc cgtcaactgc tttatcgccg acgcgccgat agccgtcaca tagaccgcgt
     5341 tacagccatc aagaaattcg accttggcgg aaagtttgcc ctcgttgccg tccatcaatt
     5401 cgtcggcaaa ctcggccacg ccaacgagtg tcgccttgtc ggggacaacg tcatagatgg
     5461 caaagccttc ggccgcacca aaatgctgat tgacccgaac acggtccgtg gtggcgaagg
     5521 caaccctgat cacggcgaca ggctcccgct taatggacca gaccagttgc agccggcgcg
     5581 tcgacaccgg gttgcccttg gcgttggcca aggtgttgtt caacgccgac gtcgtcacgg
     5641 gattcagccc agtacagtga tcgataaggt tcgagttcat ggtgttggct ctgcatcagg
     5701 ttagcgagat caaacaaggc ctgccgtgtg ccacggtagc ctacccaggt gcgggcataa
     5761 ccgcccacgt ggtcgtactg cgggaatccg gcacgcaaca acggcaggcc aagccgacgc
     5821 gccgtttcgg cggcgtgcga attggcgatg agcagttgtg cgtgcgaagc gctcgcctgt
     5881 ttttccaggt cctccagatc gccgacaatg acatctggaa tcggcaggct ggccaggctc
     5941 tccgccttgg ccggactgac aatgctgacc atttcggcgc ccatgccagt caaaaagcga
     6001 accagcatgc ccagcagatc agggtcggca gccagcgcga tgcgtgcgaa gcccagcgca
     6061 aagtgacaat ccaccatcgc gtcctgcaac tgtgcacggt gacgttcgag cttcgccggc
     6121 accggtttgc cggagatatc ggccagggcc tgggtgaagg catcgcaatc gtccagcccc
     6181 atcaagccgg caaagcggta atcgggaaca ccggttcgtg tcttcaggat cgcggcggcc
     6241 ttatcgagcg acgggccaat caccagtgtg g
//
LOCUS       DAORPM010000138         6248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3357_length_6248_cov_8.839658, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000138 DAORPM010000000
VERSION     DAORPM010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3357_length_6248_cov_8.839658"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PLD53_07715"
     CDS             complement(<1..1291)
                     /locus_tag="PLD53_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC60837.1"
                     /translation="MFKTLARILFKALFRVQLSGQPSTFINTRTLIVANHESFIDGLL
                     LGLMMPVEAVFVVHTQIANRPLFRFLLRFVPHLAVDSTSPLAMKQIVKLVETGQPVVI
                     FPEGRITKTGSLMKVYDGAAFVAAKTGATIVPVRIDGAARSYFGRLAGVYPRKLFPKV
                     TISIQPRRHIPMPDLPSAKLRRRRAGELLRQILLDMLVATRPQRTLFEAFLEGKETFG
                     ANYKLVEDVRLVEESYGSLLKMALGMMRLMSRLTAPGEVVGVLTPNAAPTLGLVLALS
                     AGRRVPALLNYTAGSDGLQAACIAANIKTIVSSRGFLEKARLTQVVEKLSGIRIEYLE
                     DLKLTIGLNDRLWVLWHLAFPGGAALAQVPDDPAIVLFTSGSEGKPKGVVHSHTSVLS
                     NVAQIRAVADFTPHDKFMMALPLFHSFGLTCGVLLPLV"
     gene            complement(1311..2141)
                     /locus_tag="PLD53_07720"
     CDS             complement(1311..2141)
                     /locus_tag="PLD53_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60838.1"
                     /translation="MKPWRKHLPLLVTVLLSGAALVHGPIAQLPDYHDFADQTRLFGI
                     PHFADVFSNLGFALVALWGLVKLTPSGRHSRIENGWAGYRLFLMGLLLTAIGSSYYHL
                     DPDNARLVWDRLPIALACGGLLAGVWGDTRQRSSTMLAAFLGVSAILSVAWWYFTEQA
                     GNGDLRPYLLLQGLPILLIPLWQWIYDMPRHDRRVFAGALLLYVIAKLAEIGDHQIAT
                     ATGAFTGHTLKHLLATGAAALIVANLIERTRVSSLAHVMPLPEASDPPNGSVAKSSSQ
                     "
     gene            2282..3016
                     /locus_tag="PLD53_07725"
     CDS             2282..3016
                     /locus_tag="PLD53_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC60839.1"
                     /translation="MNEIDQLVNTLKRRLKIQGMTYRDLAVALGVSEPSVKRMFALRR
                     FSVDRVVAISHLLGFTLAELSNEAAVSESRLHTLTEEQEGKLVSDTKLLLVAVCVLNQ
                     WEIRDIVETYALTEAECIQHLIKLDRLRLIHLLPGNRIRLNVARDFDWRPKGPIRMFF
                     LGQGLGDFLNSDFSEGDSVMAFSHAMLTEPAMVKMQKEIRKLRQKFAEIHEESLAAPL
                     SKRRGTGMLLAMREWEIGAFTKLRRN"
     gene            complement(3104..3691)
                     /locus_tag="PLD53_07730"
     CDS             complement(3104..3691)
                     /locus_tag="PLD53_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HRC60840.1"
                     /translation="MEHQLPQLPFAMDALAPHMSKETFEYHYAKHHQAYVTNLNNLIK
                     GTEYENLDLEAIVKKAPAGGVYNNSAQVWNHTFFWNCLKPNGGGAPSGALATAINAKW
                     GSFDEFKKAFQTSAVGNFGSGWTWLVKKADGTVDVVNMGAAGTPLTTGDKALLCIDVW
                     EHAYYIDYRNMRPKFVETFLNSLANWGFAEKNFAG"
     gene            complement(3722..5116)
                     /gene="xseA"
                     /locus_tag="PLD53_07735"
     CDS             complement(3722..5116)
                     /gene="xseA"
                     /locus_tag="PLD53_07735"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRC60841.1"
                     /translation="MPESTNSAVGLAAATPVIPVSMLNRLARERLESAFPLCWVAGEV
                     SNLTAAASGHVYFSLKDSVAQVRCVMFRNRAQLLGWRLENGQHIEARVLVTLYEARGD
                     FQLNIEAARKAGLGNLYEQFVRLKQKLESEGLFAVECKRRLPAFPRRIGIVTSLQAAA
                     LRDVLSTLARRASHIGLVLYPTPVQGEGAAAQIAAAIRRAGERGECDVLILCRGGGSL
                     EDLWAFNDEALARTIRSCPIPVICGVGHETDFTLADFAADQRAPTPTGAAELAAPERT
                     ALLSQLAEYRNALRRQIRQDQNQRSQHLDWLAHRLQDPAQHLARQREKLLNLQRRLDS
                     GLLQTSTRCRTRLTGLSHRLFLARPDTSRLARRLEALMPRLHGQWRTNLHAKSTDLAR
                     LAASLTLLNPDAILARGYSIVTDKAGSILRDSRNLEPNDHIVVSFRSGRVEATVTSVA
                     VEALQPNAQDRPTR"
     gene            5433..6041
                     /locus_tag="PLD53_07740"
     CDS             5433..6041
                     /locus_tag="PLD53_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRC60842.1"
                     /translation="MFSIILAAGWPIWPLLLASIIAVALIIERMVALRRVKVLPAGLL
                     QRVVAEYRKNGVSDVMLANLEAQSPLGRVLAAGLRNVGSSREVMRDAIEETGGVVAHE
                     LERYLTTLGTIASISPLMGLFGTVVGMIEIFGSQSPTGSNPMQLAHGISVALYNTGFG
                     LVIAIPGMIFWRHFRALVNGFVIEMQQQALRLVEVLHNERKL"
     gene            6052..>6248
                     /locus_tag="PLD53_07745"
     CDS             6052..>6248
                     /locus_tag="PLD53_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRC60843.1"
                     /translation="MNFQRGRGHDVPEINLIPLIDVLLVIIIFLMLTTTYAKFSGLEI
                     NLPTADASKQPEQPNEVSVAVT"
BASE COUNT         1325 a         1649 c         1912 g         1362 t
ORIGIN      
        1 acaccagcgg cagcaggacg ccacaggtca gaccaaacga atggaacagt ggcagggcca
       61 tcatgaactt gtcgtgtggc gtgaaatcgg cgacagcacg tatctgggcg acgttcgaga
      121 gtaccgaggt gtgcgaatgg acaacgcctt tgggtttgcc ctccgagccc gaggtgaaga
      181 gaacaatcgc cggatcgtcc ggaacttgcg ccagcgcagc acctccgggg aaagcaaggt
      241 gccagagcac ccacaaccgg tcgttcaacc cgatcgtcag cttgaggtct tcgaggtact
      301 cgatgcgaat tccggacagt ttttcgacga cttgcgtgag ccgggctttc tccagaaagc
      361 ctctggagga aacgatggtc ttgatgttgg cagcgatgca ggcggcttgc agaccgtccg
      421 aacccgccgt gtaattgagc agcgccggca cgcgcctgcc tgcggacagg gcgagaacca
      481 gtccgagcgt cggtgcggca ttgggcgtca gcacaccgac gacttcgcct ggggcagtca
      541 gacgggacat cagccgcatc atgccgagcg ccatcttgag cagtgagccg taggattcct
      601 cgaccaggcg aacgtcttcc accagcttgt agttcgcgcc aaaggtctcc ttgccctcca
      661 gaaaggcttc gaacagggtc cgttgcggac gtgtggcgac cagcatgtcg agcaggatct
      721 ggcgcagaag ctctccggcg cgacgacggc gcagcttggc ggagggcagg tcgggcatgg
      781 gaatatgtcg gcgcggctgg atcgaaatcg ttactttggg aaaaagtttg cgcgggtaga
      841 caccggccag ccggccgaaa tagctgcgtg cggcgccgtc aatgcgaacc ggtacgatgg
      901 tggcaccggt cttggcggcg acgaaggctg cgccgtcata cactttcatc agcgagccgg
      961 ttttggtaat tcggccttcc ggaaagatca ccaccggctg tccggtttcc accagcttga
     1021 cgatctgctt catcgccagc gggctggttg aatcgaccgc caggtgtgga acaaagcgga
     1081 gaagaaagcg gaacaggggc cgatttgcga tttgtgtatg cacgacgaag acggcttcga
     1141 ccggcatcat caagccaagg agcagaccgt cgataaagga ctcatgattg gccacgatca
     1201 gcgtcctggt gttgatgaat gtgctcggtt ggccgctgag ctggacacga aagagtgcct
     1261 tgaagaggat gcgcgctaag gtcttgaaca tgattattcc ccgatcattc ttattgagaa
     1321 ctgctttttg cgactgatcc gtttggcgga tcgcttgctt ccggcaaagg catcacgtgc
     1381 gcgagtgacg acacccgagt gcgctcaatg aggtttgcga cgatgagtgc ggcggctccg
     1441 gtggccagca gatgcttcag tgtgtgcccc gtaaatgctc ctgttgctgt cgcaatctgg
     1501 tggtcgccga tctcggccag cttggcaatg acatatagta gcagcgctcc ggcaaatacc
     1561 cggcgatcat gtcgaggcat gtcatagatc cactgccaga gcggaatgag caggatgggc
     1621 aagccctgta gaagcaaata cggacgcagg tcgccgttac ccgcctgttc tgtgaaatac
     1681 caccaggcca cactcagaat ggcggaaacc ccaaggaatg ctgcaagcat ggtgctgctc
     1741 ctttgccggg tgtcgcccca gacgccggca agcaaaccac cacaagccag cgcgatgggc
     1801 aggcgatccc agaccaggcg tgcgttatcc ggatcgagat ggtagtacga cgatccgatt
     1861 gcggtcagca aaagacccat cagaaacagc ctgtagcctg cccagccgtt ctctatccgg
     1921 ctgtgtcgac cggacggggt cagcttcacc aggccccaca gggcaaccag tgcaaacccg
     1981 agattcgaga agacatcggc gaaatgtgga atgccaaata gccgcgtttg atcggcgaag
     2041 tcgtgatagt cggggagttg cgcaatcgga ccatgcacca gggcggctcc tgaaagcaac
     2101 acggtaacga gcagaggaag atgcttgcgc cagggtttca tgtggtttct cttgagtgtg
     2161 cgaggactca gaatgcacat aagtttataa aaaataaacc agaaattaaa aagaatcgcc
     2221 atataaagca aaaggtatca gtatacgata ccgagttaca ttgccgagtg cctcaaatgc
     2281 catgaacgaa atcgatcagt tggtgaatac cctcaagcgc cgtctcaaga ttcagggcat
     2341 gacctaccgc gatctggcgg tggcgttggg tgtttcagag cctagtgtaa aacgcatgtt
     2401 cgctctgcgc cgattcagcg ttgaccgcgt cgtcgcgatc agccatttac tcggatttac
     2461 cctggcggaa ctgtcgaatg aagccgcagt gagcgaaagc cgtttgcata ccctgactga
     2521 agaacaggag gggaagttgg tgtccgacac gaagcttctt ctggttgcgg tttgcgtgct
     2581 caatcagtgg gaaatccggg acattgtaga aacctatgcg ctgacggaag cggaatgcat
     2641 tcagcatctc atcaagcttg accgcttgcg gctgatccat ctactgcccg gcaaccggat
     2701 caggctcaac gtggcgcgtg atttcgactg gcggccaaaa gggccaatcc gcatgttttt
     2761 tctgggacag ggactagggg attttctgaa cagcgatttc tcggaaggcg actcggtcat
     2821 ggctttctcg catgcgatgc tgaccgaacc ggcgatggtg aaaatgcaga aggaaattcg
     2881 caagctgcga cagaaattcg cagagattca cgaagaaagc ctggcagcac cgctatcgaa
     2941 acgtcgcggt acgggaatgc tgctggcgat gcgcgagtgg gagatcggcg cgtttacgaa
     3001 gttgcggcga aactgaatgg gacgattgag tgccaaaaaa aatctggtga gcatcatgct
     3061 ccccagattt ttagggaact agttcctgcc aataatcagg caattatcca gcaaagtttt
     3121 tctctgcaaa gccccagttg gccaggctgt tgaggaaggt ctcaacgaat ttcggacgca
     3181 tattcctgta gtcgatgtag taggcatgtt cccacacgtc gatgcacagc agtgccttgt
     3241 cgccggtagt cagtggggtg ccggcggcgc ccatgttgac tacatcgaca gtaccgtctg
     3301 ccttctttac cagccaggtc cagccggaac cgaagttgcc gacggctgaa gtttgaaagg
     3361 ctttcttgaa ttcatcaaag gagccccact tggcgttgat cgccgtcgcc aaagcgcccg
     3421 agggtgcacc tccgccgttg ggtttgaggc agttccagaa gaaggtgtgg ttccatactt
     3481 gagcggagtt gttgtagacg ccgccggccg gtgccttctt gacgatggct tcaaggtcga
     3541 ggttttcgta ttcggtgccc ttgatcaggt tgttcaggtt ggtgacgtag gcctgatgat
     3601 gtttggcata gtggtactcg aaggtttctt tcgacatgtg gggtgccagt gcgtccatcg
     3661 cgaaaggcag ttgcggcagt tgatgttcca tcatttctcc ttggctttct ttacgggggt
     3721 gtcagcgggt cgggcggtct tgcgcgttcg gttgaagcgc ttcaacggcc accgaagtaa
     3781 ccgttgcctc tacccgacca ctgcggaagc tgacaacgat atggtcgttt ggttccagat
     3841 ttcgactgtc tcgcaggata ctccctgctt tgtcggtgac gatgctgtaa ccgcgggcca
     3901 gtatggcgtc tggattgaga agggtcaagc tggcagcaag gcgggcgagg tcggtagact
     3961 tggcgtgcag gtttgtccgc cattgaccgt gcagtctggg catcagtgcc tcaagtcgcc
     4021 gggcgagccg gctggtatcc ggccgagcca ggaataggcg gtggctcagg ccggtgagcc
     4081 tggttcggca gcgtgtgctg gtttgcagca ggccactgtc caggcgtcgc tgcaggttga
     4141 gcagcttttc tctttgtcgt gccagatgtt gcgctggatc ttgcagtcga tgcgccagcc
     4201 aatcgagatg ctggctgcgt tggttctggt cttgtcggat ctggcggcgt agcgcattcc
     4261 ggtactccgc aagctgcgac agcagtgcag ttcgttccgg cgccgcaagc tcagcagctc
     4321 ctgttggggt gggagcgcgc tgatcggcgg cgaaatcagc caaagtgaag tcggtctcat
     4381 gaccgacccc gcagattacc gggattgggc atgagcggat ggtgcgagcc agcgcttcgt
     4441 cattgaatgc ccacagatct tcgaggctgc ctcctcccct gcagaggatg agcacgtcgc
     4501 actcaccgcg ttcgcctgcg cggcgaatgg cggcggcgat ttgtgcggct gcgccttcac
     4561 cttgaaccgg cgtagggtac agcacaagac cgatgtgtga tgcgcgacgc gcgagcgtgc
     4621 tgagaacgtc tctgagcgcg gctgcctgga gtgaggtgac gatgccgatg cggcgcggaa
     4681 aggcgggcag cctgcgcttg cattcgacag caaaaagtcc ttcgctttca agcttttgct
     4741 tgaggcggac gaactgttcg tacagattgc caaggcctgc cttgcgcgcc gcttcgatgt
     4801 tgagctggaa gtccccccgt gcctcgtaca gcgttaccag aacccgcgct tcgatgtgct
     4861 ggccgttctc cagccgccag ccaagaagct gggcgcggtt gcggaacatg acgcaacgta
     4921 cctgtgctac cgaatccttc aatgaaaaat agacgtgtcc agaggctgcg gcagtcaggt
     4981 tggatacttc gcccgcgacc cagcagagcg ggaaagctga ttccagtcgc tcacgcgcaa
     5041 gccggtttag cattgaaacg gggatgacgg gtgttgccgc ggcaaggccc acagctgagt
     5101 ttgtgctttc tggcatgcgc caattctaca tccgtgttgg ttcatgcaca ccctgcgggt
     5161 gtttcagggc aaagttcggc tgaaaccgtt gtggcgtcag aggattttgt taagtgattg
     5221 attgtaaata gaattaggat cggttgatat ttccggcaaa gttggaaaag tccttttgcc
     5281 aagggcactt agcgttcacg agcgggtttt atccacagcg ttgtccacag attggtcgaa
     5341 taagaattct gacattactt tgggcgcagg gcttgccgct ggcgtcggct caggctaaag
     5401 tctccggctg aaaagatctt tgaggggaaa gcgtgttctc aatcattctg gctgccggct
     5461 ggcccatctg gcccttgttg ttggcgtcaa tcattgccgt cgccctgatc atcgaacgca
     5521 tggttgcgtt gcgccgtgtc aaggtgcttc ctgctggttt gctacaacgc gtcgtcgccg
     5581 agtaccgcaa gaatggcgtc agtgatgtca tgttggccaa ccttgaagcg caatcaccgc
     5641 ttggtcgcgt actcgccgcc ggattgcgca atgtcgggag ttcgcgcgag gtcatgcgcg
     5701 atgcaatcga agaaaccggc ggagttgtgg ctcatgaact tgagcgctac ctgacgacgc
     5761 tgggcaccat cgcatcgatc agccccctaa tgggtttgtt cggcaccgtg gtcggcatga
     5821 tcgagatttt cggttcgcag tcaccgacgg gatcaaatcc gatgcaactt gctcacggta
     5881 tatcggtggc gctttacaac acgggttttg gtttggttat cgccataccg ggaatgattt
     5941 tctggcgcca ctttcgcgct ctggtcaatg gcttcgtaat cgaaatgcag cagcaagcct
     6001 tgcgtttggt cgaggttctg cacaacgagc gaaagcttta aggagcaagg aatgaatttc
     6061 cagcgtggcc gtggccatga cgtacctgag atcaacctga ttccgctgat cgacgtgctg
     6121 ctggtgatca ttatttttct gatgctcacg actacctacg ccaagttctc ggggctggaa
     6181 atcaacctgc cgacggccga cgccagcaaa cagcccgagc aacccaatga agtcagcgta
     6241 gccgtaac
//
LOCUS       DAORPM010000139         6237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3366_length_6237_cov_12.039469, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000139 DAORPM010000000
VERSION     DAORPM010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3366_length_6237_cov_12.039469"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /gene="gcvPB"
                     /locus_tag="PLD53_07750"
     CDS             <1..1475
                     /gene="gcvPB"
                     /locus_tag="PLD53_07750"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRC60844.1"
                     /translation="HTEHEMLRYLKRLQNKDLALDSSMISLGSCTMKLNATSEMIPVT
                     WPEFGDLHPFAPLDQAQGYLEMIGELENWLKVITGFDAVCMQPNSGAQGEYAGIVAIS
                     RYHASRGEGHRNICLIPKSAHGTNPATAQMCGLQIVVVDCDNNGNVDVTDLKAKAEKH
                     AARLSCLMITYPSTHGVFEEAIRDICAIVHSHGGQVYMDGANLNAQVGLTSPGLVGAD
                     VSHMNLHKTFAIPHGGGGPGMGPIGLKAHLAPFMADHVVQATGGAERLHRGQGAVSAA
                     PWGSASILPISWMYIAMLGGKGLKRATEVAILNANYVAARLNEHYPVLYTGKNGRVAH
                     ECILDIRPIKAATGGNSGIAEIDIAKRLMDYGFHAPTVSFPVAGTIMVEPTESESKVE
                     LDRFIAAMIAIREEIRKIEQGVWPVDNNPLKNAPHTQADIVVAKWDRPYSREDAVFPL
                     PWVASNKFWPSVNRIDDVYGDRNLFCACVPIGDYQESQES"
     gene            1477..>2513
                     /locus_tag="PLD53_07755"
     CDS             1477..>2513
                     /locus_tag="PLD53_07755"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003470.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HRC60845.1"
                     /translation="MRIEQDMKLDFKDVLIRPKRSTLTSRSEVDISRDYVFLHSKRKY
                     QGIPIIASNMDATGTFDMARALAAHQLSVALHKYYDEAELLAFFAEPSGSSASFYSMG
                     ITLPDYEKFRRVKEQAGDKIEYVCVDVANGYTKALVGFIQKLRSAYPDITLMAGNVVT
                     GEMTEELILDGVDIVKVGIGPGSVCTTRRVSGVGYPQLSAIIECADAAHGLHGLICAD
                     GGCTSPGDLAKAFGAGADFVMLGGMLAGHDECGSDIVEQDGMLKMRFYGMSSREAMEK
                     YAGGVADYRAAEGKDVLLDYRGPVENTVQEILGGVRSACTYVGARKLKELSKRTTFIR
                     VTQQLNEVFGS"
     assembly_gap    2514..2613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2625..3095
                     /locus_tag="PLD53_07760"
     CDS             2625..3095
                     /locus_tag="PLD53_07760"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRC60846.1"
                     /translation="MSAFSAERLQGTNQPFADYAGKILLIVNTASKCGFTPQYTGLES
                     LYQRFRDRGLLVLGFPCNQFGAQEPGDASEIASFCQTNYGVSFQMFAKVDVNGDNAHP
                     LYRYLKKSAPGLLGSEAIKWNFTKFLLNRKGEVTKRYAPATTPDAIAEDIETLL"
     gene            complement(3113..4039)
                     /locus_tag="PLD53_07765"
     CDS             complement(3113..4039)
                     /locus_tag="PLD53_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRC60847.1"
                     /translation="MRFTLRQMTVFVAVARQESVSNAAKELSLSQSATSTSLGELERL
                     YDTQLFDRIGKALRLNELGQSLLPQAVELIERAAAIEAALERRAGFGRLRIGATLTIG
                     NYLATLIVADYLKRHPESSAQLQVHNTATIIDQVARYDLDLGLIEGNCRHSDLVAEPW
                     VADELVVFCAPTHRLATKGVATLADLADEHWIVREPGSGTRETLDQAMRLHHFELRAR
                     LELEHTEAIKRAVEFGLGIGCISRLALREAFRRGSLVAIETPELELLRHFQFLWHKRK
                     FQTAGMQEFISLCRGMTAGIRRSDQIELPHIP"
     gene            4138..4914
                     /locus_tag="PLD53_07770"
     CDS             4138..4914
                     /locus_tag="PLD53_07770"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRC60848.1"
                     /translation="MSNLSSQRVLSVHHWNDNLFSFRTTRDASLRFINGQFVMLGLEQ
                     EGRPLTRAYSIASANHDDFLEFFSIKVTNGPLTSKLQHLSVGDAVVVSRKPTGTLVLR
                     DLKPGKNLYLLATGTGLAPFISLIQDPESYERFDKVILVHGVRTVNELAYRDFITEEL
                     PKHEYFADLVQDKLIYYPTVTREPFQNQGRITHLIENGKLFSDIGLPTLNPAKDRVMI
                     CGSPAMIKDCCTLLNERGFKMSPHIGATGDYVIERAFVEK"
     gene            5002..5544
                     /locus_tag="PLD53_07775"
     CDS             5002..5544
                     /locus_tag="PLD53_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRC60849.1"
                     /translation="MSSSNISLVLTAIGDDRPGLVEQLATAISRHQGNWLESSMSHLS
                     GKFAGIVRVSVPGTQLEALKSALAELTGLRVTSEACEPSPSTETAAHTRRLKFSLVGH
                     DRIGIVREVSQVFARHEVNVEDLSTHTASAPMSAEILFHATAALTASSTLDVGALTDD
                     LESISNDLMVDITLDETIPT"
     gene            5606..>6237
                     /locus_tag="PLD53_07780"
     CDS             5606..>6237
                     /locus_tag="PLD53_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC60850.1"
                     /translation="MAQYVFTMNRVGKIVPPKRQIIKDISLSFFPGAKIGLLGLNGSG
                     KSTVLRIMAGLDKDIIGEATPMPGLNIGYLPQEPQLDPELSVRQEVESGMGEVMQAQE
                     KLEAVYAAYAEEDADFDKLAEEQARLEAIIAAAGNDLASQLELAADALRLPPWDAPIK
                     NLSGGEKRRVALCKLLLSRPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTV"
BASE COUNT         1339 a         1869 c         1708 g         1221 t
ORIGIN      
        1 atcacaccga acacgagatg ctgcgctacc tcaagcgctt gcagaacaag gatctggccc
       61 tcgacagctc gatgatctcg ctgggttcat gcacgatgaa gctgaacgcc acgagtgaaa
      121 tgataccggt cacttggccc gagttcggcg acctccaccc cttcgccccg ctcgatcagg
      181 cgcagggcta cctggaaatg atcggcgaac tggaaaactg gctcaaagtc atcaccggtt
      241 tcgacgcggt ctgcatgcaa cctaactccg gcgcacaggg tgaatatgct ggaatcgttg
      301 ccatttcgcg ctaccacgcc agccggggcg agggccaccg caatatctgc ctgatcccga
      361 agtcggcgca cggcaccaat ccggcgaccg cgcagatgtg cggcctgcag atcgttgtcg
      421 tggattgcga caacaatgga aatgtcgacg ttaccgacct gaaagccaag gccgaaaagc
      481 atgcagcccg gctatcctgc ctgatgatca cctatccctc gacacacggc gtattcgaag
      541 aggcgatccg ggacatctgc gcgatcgttc attcgcacgg tggccaagtg tatatggatg
      601 gggccaacct caacgcgcag gtcggactca cctcgccggg attggtcggc gccgatgtga
      661 gccacatgaa tttgcacaag accttcgcca tcccccacgg cggcggtggt ccgggcatgg
      721 gaccgatcgg cctcaaagcc catctggcgc cgttcatggc ggatcacgtg gtgcaagcga
      781 cgggcggggc ggaacgtttg catcgcggac aaggcgcggt ctcggcagcg ccgtggggtt
      841 ccgcatcgat cctgccgatt tcctggatgt acatcgccat gctgggcggc aagggcctca
      901 aacgggcaac agaagtggcg attctcaacg ccaattacgt cgccgcccgt ctcaacgagc
      961 attacccggt gctctacacg ggcaagaacg gccgcgttgc ccacgagtgc atactcgaca
     1021 tccgaccgat caaggcagcg acgggcggca attcggggat cgccgaaatc gacatcgcca
     1081 agcgactgat ggactacggc ttccatgcgc caacagtcag cttcccggtc gccggaacga
     1141 tcatggtcga gccgaccgag tcggagtcaa aagttgaact tgaccgcttc atcgctgcaa
     1201 tgattgccat ccgcgaggaa atccgcaaaa tcgaacaagg tgtctggccg gtagacaaca
     1261 atccgctcaa gaacgcgcca catacgcagg ccgacattgt cgtcgccaag tgggaccgcc
     1321 cctacagccg tgaagacgcc gtatttccgc tgccatgggt ggcatccaac aagttctggc
     1381 cgagcgtcaa tcgcatcgat gatgtgtatg gtgaccggaa tctgttctgc gcctgtgtgc
     1441 cgatcggaga ctaccaggag agtcaggaga gctgagatgc gtatcgaaca ggacatgaaa
     1501 ctcgatttca aggacgttct catccgtccg aagcgctcga cgctcacctc gcgctcggaa
     1561 gtcgacattt cccgcgatta cgtttttctg cattccaagc gcaaatatca gggcataccc
     1621 atcatcgcct ccaacatgga tgcgaccgga acatttgata tggcgcgggc gctggccgcc
     1681 catcagctct ccgtcgccct ccacaaatat tacgacgaag cggagttgct cgcctttttt
     1741 gccgaacctt cgggttcttc ggcttcgttc tattcgatgg gcatcacgct gccggactac
     1801 gagaagttcc gtcgcgtcaa ggagcaagcc ggagacaaaa tcgaatacgt ctgcgttgac
     1861 gtcgccaacg gctataccaa ggctcttgtc ggctttattc agaaattgcg cagcgcctac
     1921 ccggatatca ccctcatggc cggcaatgtc gtgactggcg aaatgaccga ggaattgatc
     1981 cttgacggtg tcgatattgt caaggtcggg attggcccgg gctcggtgtg cacgacgcgc
     2041 agggtgtccg gcgtcggata cccccagctc tcggcgatca tcgaatgtgc cgatgcggcg
     2101 cacggcctgc acggtctgat ctgcgctgac ggcggttgta cttcaccagg agacttggcc
     2161 aaggcttttg gtgctggcgc cgatttcgtc atgcttggcg gcatgctggc cggtcacgat
     2221 gaatgtggtt cggacatcgt cgagcaagat ggcatgctca aaatgcgttt ttatggcatg
     2281 agttcgcggg aagcgatgga aaagtatgcc gggggcgtcg ccgactaccg cgccgccgaa
     2341 ggcaaagatg ttctgctgga ttatcggggt cctgttgaga acaccgtgca ggagattctg
     2401 ggcggagttc gctcggcctg cacttacgtc ggcgcgcgaa aactcaaaga gttgtccaag
     2461 cggacgacct ttatccgggt tacccagcaa ctgaacgagg ttttcgggtc gaannnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncagacta cccactgtcc gcgttttcgg
     2641 cggaacgatt gcaggggaca aaccagccat tcgccgacta tgcgggaaag attctcctca
     2701 tagtaaacac cgccagcaaa tgtggtttca cgccacaata caccggtctc gaatcactct
     2761 accagcggtt ccgggatcgc gggttgctgg tgctcggttt cccgtgcaac cagttcggcg
     2821 cgcaggaacc gggcgacgct agcgaaatcg cgagtttctg ccagacgaac tatggcgtca
     2881 gttttcagat gttcgccaag gtcgatgtca acggcgacaa tgcccacccg ctctatcgat
     2941 atctgaagaa atcggcgccc gggctgctcg gcagcgaagc tatcaaatgg aacttcacaa
     3001 aattcctgct caaccgcaaa ggtgaagtga ccaaacgtta cgcgccggcg acgactccgg
     3061 atgccattgc cgaggatatt gaaaccttgc tctgaatcgg gctcgccgct cgctacggga
     3121 tatgcggcag ttcgatctgg tcgctgcgcc ggatacctgc ggtcatgccg cggcatagcg
     3181 agatgaactc ctgcattccg gccgtctgga atttgcgttt gtgccagaga aactgaaaat
     3241 ggcgcagcaa ctcgagttcc ggcgtttcaa tggccaccag actgcctctc cggaacgcct
     3301 cgcgcagtgc caaccgtgat atgcagccga ttccaagccc aaactcgacg gcccgcttga
     3361 tcgcttcggt atgttccagt tccagccggg cccgcaactc gaaatgatgg aggcgcatcg
     3421 cctgatccag cgtttcgcgc gtccctgaac ccggttcgcg aacgatccaa tgttcgtccg
     3481 caaggtcagc cagcgtcgcc acacccttgg tcgccaaacg atgcgtaggc gcgcagaaca
     3541 ccaccagttc gtccgcaacc cagggttcgg caaccagatc ggagtgccgg caattgcctt
     3601 cgatcaggcc cagatcgaga tcgtatcgcg ccacctgatc gataatcgtt gccgtgttgt
     3661 gcacctgcaa ctgcgcggag ctctcgggat gccgcttgag atagtcagca acaatcagcg
     3721 tcgccagata gttgccaatg gtcagggttg cgccaattcg cagcctgccg aagccagccc
     3781 gcctctccag ggcggcttca atggctgctg cgcgttcaat caattccacc gcctgcggca
     3841 acaaggattg cccgagttcg ttgagacgca gggccttgcc gatgcggtca aaaagctggg
     3901 tatcgtagag acgctccagt tcgcccagcg aggtactggt cgccgattgc gacagcgaca
     3961 actcctttgc tgcattggaa acactttcct gtcgggcaac cgcgacgaat accgtcatct
     4021 gacgcaaagt aaatcgcata tctactccat agatatttct tatctaaaca atctgtttta
     4081 cggatatatt agcgtggact accattcagt tcatcaaaac cgtcgagaaa caaggccatg
     4141 agcaatcttt cctcccagcg cgtcctttcc gtccatcact ggaacgacaa cctgttcagc
     4201 ttccgcacca cacgcgatgc gagcctgcgc ttcatcaacg gacagtttgt gatgctcggt
     4261 ctcgaacagg aaggacgtcc gctaacccgc gcctacagca ttgccagcgc caatcacgac
     4321 gattttctgg aatttttcag catcaaggtc accaacggcc ccctgacatc gaagctgcag
     4381 catctatccg tgggcgacgc cgtcgtcgtc agccgcaagc ccacgggaac gctggtcttg
     4441 cgtgacctta aacccggcaa gaacctctat ctgctggcca cgggtaccgg tctcgcgccg
     4501 ttcatcagcc tgattcagga tccggaaagt tacgagcgct tcgacaaagt tattctggtt
     4561 cacggggtgc gcacagtgaa tgaactggcc taccgcgact tcattaccga agaactcccg
     4621 aagcacgaat acttcgccga tctggtacag gacaaattga tctattaccc gaccgtcacg
     4681 cgcgagccct ttcagaatca gggtcggatc acacatttga tcgaaaacgg gaagctgttt
     4741 tccgatatcg gcctgcccac gctgaacccc gccaaggacc gcgtcatgat ctgtggcagt
     4801 ccagcgatga tcaaggattg ctgcacactg cttaacgagc gcggattcaa gatgtcgccc
     4861 cacattggcg ccacgggtga ctatgtcatc gagcgtgctt tcgtcgaaaa atagggcatc
     4921 cacccggcac aacgcgccaa gcatgaactg aaggagtcgg cccggcgtac aatggcggca
     4981 ttactgcgtc cggagtacgc catgagcagc agcaatatct ctctcgtttt gaccgccatc
     5041 ggcgatgatc gtccgggcct ggtcgagcaa cttgccaccg ccatcagccg acatcaggga
     5101 aactggctgg aatcatcgat gtcgcacctg tcggggaaat tcgccggtat cgtgcgcgtc
     5161 agcgtccccg gtacgcaact cgaagcgctc aagtcggcgc ttgcagaatt gaccggcctg
     5221 cgtgtcacct ccgaagcgtg cgaaccatcg ccttccacgg agacagccgc acatacccgg
     5281 cggctgaagt tttctctggt tggccatgat cgtatcggga tcgtgcgcga ggtttcgcaa
     5341 gttttcgccc ggcacgaggt caacgtcgaa gatctcagta cacatacggc cagcgcgccg
     5401 atgtcggcag aaatactttt ccatgccacg gccgcactga cggcctcgtc gacgctggat
     5461 gtcggcgccc tgaccgatga tctggaaagc atctcgaacg acctgatggt cgatatcacc
     5521 ctggacgaaa ctatccccac ttgatgaaat tgagataccg gtcagcgtat cgtcagtttc
     5581 agttattgtc acaaagaacc cagccatggc tcagtacgta tttacgatga accgcgtggg
     5641 caagatcgtc ccgcccaaac gccagattat caaggacatc tccctttcct tctttcccgg
     5701 cgccaagatc ggcctcctgg gactcaacgg ctccggcaaa tcaaccgtgc tgcgcatcat
     5761 ggccggcctc gacaaggaca tcattggcga agcaacgccg atgcccggcc tgaacatcgg
     5821 ctacctgccg caggagccgc agctcgatcc cgagttaagc gttcgccagg aagtcgagtc
     5881 gggcatgggc gaagtcatgc aggcgcagga aaaactcgaa gccgtctatg ccgcttacgc
     5941 cgaggaggat gccgatttcg acaagctggc ggaggaacaa gcgcggcttg aagcgatcat
     6001 cgccgcagcc ggcaacgacc tggccagcca gctcgaactc gccgccgacg cgttgcgcct
     6061 gccaccctgg gacgcaccca tcaagaacct ctccggtggc gagaagcgcc gcgttgccct
     6121 gtgtaagctg ctgctctccc ggcccgacat gctgcttctc gacgagccga ccaaccacct
     6181 cgacgccgaa tcggtcgaat ggctggagca gttcctcgta cgctttcccg gcaccgt
//
LOCUS       DAORPM010000140         6186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3410_length_6186_cov_8.398793, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000140 DAORPM010000000
VERSION     DAORPM010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_6186_cov_8.398793"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLD53_07785"
     CDS             complement(<1..360)
                     /locus_tag="PLD53_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60851.1"
                     /translation="MSSELEEDFEIEDPAEELFRACEKVLRSGIWLIRNGYGRLAILP
                     YLSPSGCHWRCEFHPVGFRSRTMFRYTSADGHKYLAAHCGGTLRKDASPEKLAKAALV
                     GADEDLLSACSGEASPET"
     gene            complement(517..1542)
                     /locus_tag="PLD53_07790"
     CDS             complement(517..1542)
                     /locus_tag="PLD53_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60852.1"
                     /translation="MSDVPGATVPASRLSTVLENIEQDKKLSANAFEYLRQQGYLALL
                     SLASGESNYQEFSNLARVEQGRRESAAAEVRQKEEAERLRAIAESAAKEAARTAEYER
                     QRRIQESDPKYVAKMKNQRLRARYGLDQFIEKESFNRLMGILHRIDGGARFSDEDVLW
                     LTTEGSDYYSDELRFEFHSREAAFFVTEFARTRDPWNAVNASGHFRKCDRSGAAHELL
                     EGVSLEGLKSPKLKSAVRTTHGGVMRDLGRFDVALRYGTEAHAITDSDFRPCTLLGAV
                     NFELGNYESARDWYAKAAERGASERSIDDELRGIYMRADKSKRDEIRAFLIREDPVRY
                     KWAHSLR"
     gene            complement(2094..2615)
                     /locus_tag="PLD53_07795"
     CDS             complement(2094..2615)
                     /locus_tag="PLD53_07795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRC60853.1"
                     /translation="MSEAPFGIESLNAGHDRLSFRSGSDPLDRYFREQATQDVKRRVA
                     ACFVAVDDSRAIAGYYTLASAGILLADLPHELGKRLPRYPTVPAVRMGRLAVSQNFKG
                     MGLGGALLADALNRSACSEIAAYALVVDAKDGEAAGFYLHHGFVALPSTPLTLFLPLA
                     TALASRKVGKAAQ"
     gene            complement(2612..2893)
                     /locus_tag="PLD53_07800"
     CDS             complement(2612..2893)
                     /locus_tag="PLD53_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HRC60854.1"
                     /translation="MSAAVSTARLEARISADLHSMLKRAAELQGRTMTDFVVTAVQAA
                     ARSAIEQAEVVRMSLADQECFAQALLSPPSPSPALDRAFARRSKLLRSE"
     gene            complement(3263..3853)
                     /locus_tag="PLD53_07805"
     CDS             complement(3263..3853)
                     /locus_tag="PLD53_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60855.1"
                     /translation="MLKHIWGHQIPRVLREPALFDKTGLPKYWATVWSMFHTADLAPS
                     SKRKVLSHVEALYQFSETLHGPGMLDDALFSVDIEKLGQLLKAYFVSIRNLPNINEAA
                     QLKWQTAFRFVSDIIRRLSKSDLPIAQLQQFQGRLIGLTLARLSAPVTYSSNKFRLFG
                     VGPYERKNRIHEPAGGVANSWSGMIGSSMAESDSFG"
     gene            4457..5965
                     /locus_tag="PLD53_07810"
     CDS             4457..5965
                     /locus_tag="PLD53_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60856.1"
                     /translation="MNAPLLERYGDRIAGVLTCYDRMVITGTLPGPCYAAGMTSFLNA
                     RQIRIFDYPRFAEPLRDTIRANAEQLAEAASVKIEYLAKAHIRKEDVIAGVIKTRGDH
                     PGLVHVISVMEACSTYKAWHDKRTHQTSLRPTTGKCLHYYFYFIDAELGLVYLRVPTW
                     CPFRLQFYCNGHRWLARQLTAAGVGYTLADNAFIRIDDWDRAQQLADRLSPDVLHRIL
                     DRYAQACCPVLDVFAQRYHWSLMQIECSTDLAFRSDAIMKPLYAALSREAILSVKAEQ
                     VMHFLGKKMTPQLAQEVDSRLSTRIEGICIKHRMGKNSVKMYDKFGRVLRIETTTNDV
                     SFFKHHRQVEHRDGQATRELATVKKSIYSLIDLREILLGCNRRYLEFLSALDDFSAGS
                     RRLDKLTAAKTVDGHRVKGFNFFDKTEHTLLCSLQRPQFNISGVRRADLKPFLPNLSV
                     ASITRYLKRLRVLGLIKKIAHAYRYYLTRLGRNAIAAACHLTEQIIVPALAA"
BASE COUNT         1280 a         1809 c         1763 g         1334 t
ORIGIN      
        1 ggtttccggt gaggcttctc cggagcacgc cgaaagcagg tcttcgtcgg cgcccacgag
       61 ggcggccttg gcaagctttt cgggagaggc gtccttcctg agcgttccgc cgcaatgggc
      121 ggccaggtat ttgtgtccgt cggcgctcgt gtagcggaac atggtcctcg atcggaatcc
      181 gaccggatgg aattcgcacc gccaatggca gccggacggg gagaggtacg gcaggatcgc
      241 gaggcggccg tacccgttcc ggatgagcca gatgccggag cgtaggactt tctcgcacgc
      301 cctgaagagt tcctctgcgg gatcctcgat ctcgaaatct tcttccaatt cgctcgacaa
      361 cgtccgttcc tcgtctattc gcacttggcg gcggaatgtg ccatggtaaa ccatgatgta
      421 tggattgtgc cgttggtaca aaaaccgcca ctccggcccc agtgggcggg aaaagcggtt
      481 ttcgcagacg cgacgactcg gcgtcttgga ctgcggctac cggagactat gggcccattt
      541 gtatcggacc ggatcttccc tgatgaggaa cgccctgatt tcgtcccttt tcgatttgtc
      601 ggcccgcata tagatgccgc gcagttcgtc gtcgatcgag cgttcgctcg caccccgttc
      661 cgccgccttg gcgtaccaat cccgtgccga ttcgtagttg cccagctcga aattcacagc
      721 accgagcagc gtgcagggtc ggaaatcact gtccgttatg gcatgcgcct cggttccgta
      781 gcggagcgcc acgtcgaacc ggcccaaatc gcgcatgact ccgccgtggg tcgtacggac
      841 ggcggacttc agcttcggtg atttcaggcc ttccaaggaa acgccctcca atagctcgtg
      901 agcggctccc gagcggtcgc attttcggaa atggccgctg gcgttgacgg cgttccaagg
      961 gtcgcgggtt cgggcgaatt cagtcacgaa gaacgcggct tccctggagt ggaactcaaa
     1021 cctgagttcg tcggaatagt aatccgagcc ctccgttgtt agccacagca cgtcctcgtc
     1081 actgaatcga gcgcctccgt cgatccggtg gaggatgccc atgaggcggt tgaacgattc
     1141 cttctcgatg aattggtcca ggccgtatcg ggcccggagc cgttggttct tcatcttggc
     1201 gacgtatttc ggatcgcttt cctggatccg tcgctggcgc tcgtactccg ccgtccgggc
     1261 ggcttccttg gccgcagatt ccgcaatggc ccgcagacgt tcggcttctt ccttctgcct
     1321 gacctcagcg gcggcggatt cccgccttcc ctgttcgacg cgggccagat tagagaattc
     1381 ttgatagttc gactcgcccg aagccaggct caataaggcg agatacccct gttgccgtag
     1441 gtattcgaac gcattcgccg aaagcttctt gtcctgttcg atattttcga ggacggtgct
     1501 gagccggctg gcgggaacgg tcgctcccgg gacgtccgac aagaaatact tgccggcgat
     1561 cgcggatcgt cccggatcta tgactacccg ctgttgagga cccgcttcgg gacgcccgga
     1621 gcgtctgaaa ttcggatttg gctgcctaag gattcgtggg gttctgctca tggtaagctc
     1681 ttgtcggcgc tcgtcggcag gctgccgagt attccatgcg gattctaata ccaaaggttg
     1741 ccggccgcgg catcgggagc gcgccggtac cgacgcggtt cgcccttcgc aatctaggta
     1801 atttcgggta atgatgcaca ctggatagtg tcggtataac aggatccatt gacattttgt
     1861 tgaaaactgc cgttgcgttt aaggtatccg tctattgttg cgcactctat attcgattgg
     1921 cgcaacagtt aagcccctaa ttttttcaag ttgcgcaaca cctgcagaaa gggggttgcg
     1981 cttcacactt ctagtgtcaa tcagcgggcg gcacctacaa gaaccgcgtg ggcggcgcca
     2041 tctgcatcac caacaaggcc tcttcggtgg cctttgtcat gcaagagact tgcctattgg
     2101 gcggccttgc ccaccttccg ggatgccagc gccgttgcca atggaaggaa aagagttaaa
     2161 ggcgtactgg gcagggcgac aaatccgtgg tggagataga agcccgcggc ctccccgtcc
     2221 ttggcatcaa ccaccaaggc ataggcggca atctcggagc aggccgagcg attcaaggca
     2281 tcggccagca acgcgccgcc gagccccatg cccttgaaat tctgggagac tgccagccgc
     2341 cccatgcgca ccgcgggcac cgttggatag cgtggcagac gcttcccgag ttcgtgaggg
     2401 aggtcagcca gaaggatgcc ggctgaggcc agcgtgtaat agccagctat cgcgcgtgag
     2461 tcatccaccg ccacgaagca cgccgccacg cggcgcttca catcctgcgt cgcctgttcc
     2521 cggaaatacc gatccagcgg atccgaccca ctgcggaagg acagtcgatc atgcccggcg
     2581 ttgagcgact cgatgccgaa cggagcctcg ctcattccga acgcaacagc ttgctacgac
     2641 gggcgaaggc gcgatccaaa gcgggtgaag gggatggcgg ggacagcaat gcctgggcaa
     2701 agcactcctg atccgccaga gacatccgca ccacctcggc ctgctcaatg gcggagcggg
     2761 cggcggcctg gacggcggtg acgacgaaat cagtcatggt acgcccctga agctcggcag
     2821 cacgtttgag catcgaatgc agatctgcgc tgatgcgggc ttcgaggcgg gcagttgaaa
     2881 cagctgctga catgggtgaa tcctcctagt cgaattatac ggccaactgc cgtacaaagc
     2941 caatctatca tggtgttgct gtgcacgttg gagttgagct tttccgctac tgtcagcaac
     3001 tgaggtaggc tggaaaaaat tgatgtcatc gttgatcaag ccgctgccgc gttctcggcc
     3061 agccaacgat gtccggggat gtggcgagtg ttggctgatg aaattgattg gtcatggggc
     3121 gagattgccg ccagatttct aatctgtagc gggggcgaac tggatcatcc agtcgatgaa
     3181 gctggactat aatgcgcttc gcattctggc aacgaagagt cgctctggct cttggatgtc
     3241 aggcgaggcc gtgcggcttc gttcaaccaa acgaatcgga ttcagccatg gaagatccaa
     3301 tcattccgga ccaagagttc gccactcccc ctgcgggctc gtggattcga tttttgcgct
     3361 cgtatggtcc gacaccgaac aacctgaact tgttcgacga atatgtgacc ggcgcgctga
     3421 gtcgggccaa ggtcaagccg attagccgcc cttgaaactg ttgaagctga gcgataggca
     3481 aatcgctctt gctcaatcgg cggatgatgt cgctgacgaa tcgaaaggct gtttgccact
     3541 tcaattgagc tgcttcgttg atgttcggaa gattccgaat cgaaacgaaa taagctttga
     3601 ggagttggcc cagcttctcg atatcgacac tgaacaatgc atcgtcaagc atgccgggcc
     3661 catgcaaagt ttcagagaac tggtagagag cctcaacatg actaagtact ttacgcttgc
     3721 tggatggcgc caagtctgcg gtatgaaaca ttgaccagac cgttgcccaa tattttggta
     3781 atccggtctt gtcgaaaaga gctggctcac gtagaacacg aggaatttgg tggccccaaa
     3841 tgtgttttag caactcgtca ccattcccaa gaaaaaacca atgctcaaaa acgagaccct
     3901 gtggatttgt ggacagtcgg ctacgccgat acccgcctaa ccgcgcaagc gcggttccct
     3961 atggacaagc cgtggaaaac gcgatgaacc atgcgtttcc caccacttgc ccacaggtcg
     4021 gcagctgccc tctcgctacg ttgatcgacc gcttctggcc ggttgtgtga gtacgatccg
     4081 gcgctcggta gccgttgcgc atcaatgcca aaagtattgt ccattaccat atggacagac
     4141 tggcaaaacc ttctatcccc agattttgtg gataaacctg tgagtgggct ggtggcagtc
     4201 ttctataaga cctgcctggc caaggattta ggcgacctgc ctacaaacta ggcacacctt
     4261 ttttagggaa tcgctgcgtt ggctgacact cagtctcgtt gaccatgcaa ccaagccgcc
     4321 gcagaaatcc ttggcgggag caaccaagcg accccgcgct gtttttcatc atagaacatg
     4381 acttctccta agccggacga gccggaacca aacaggttaa agtaagagcg cgctgtgacg
     4441 cgaagtggag gttgccatga acgcaccgct tcttgaacgc tatggtgacc ggatcgccgg
     4501 cgtgttgacc tgttacgacc ggatggtgat cacgggcaca ctgcccggcc cgtgttatgc
     4561 ggcggggatg acgagcttcc tgaatgcccg tcagattcgt atctttgact atccgcgctt
     4621 tgccgaaccg ttgcgtgaca ctatccgggc gaatgccgag cagttggcgg aagcggcaag
     4681 cgtgaagatt gagtaccttg ccaaggcgca cattcgcaag gaagacgtca ttgctggggt
     4741 gatcaagacg cgtggcgatc atcccgggct ggtgcatgtc atttcggtca tggaagcctg
     4801 ctcgacctac aaggcgtggc acgacaagcg cactcaccag acctccttgc gcccgacgac
     4861 gggcaagtgt ttgcattact acttttactt catcgatgcc gagttggggc tcgtctacct
     4921 gcgcgtgccg acctggtgtc cgttccgcct ccagttttac tgcaacggcc atcgctggct
     4981 ggcccggcaa ctgaccgccg ccggcgtggg ttacaccctg gccgacaatg ccttcatccg
     5041 catcgatgac tgggatcggg cccaacagtt ggcggatcgc ctgtccccgg acgtattgca
     5101 ccggattctt gaccgttatg cccaggcctg ctgtcctgtc ctggacgtct tcgctcagcg
     5161 ttaccactgg agcctgatgc agatcgagtg ttcgacggat ctcgccttcc gttcggacgc
     5221 gatcatgaag ccgctctacg cagccctttc gcgggaggcc attctttcgg tcaaggccga
     5281 acaggtgatg cactttctgg gcaaaaaaat gacgccgcaa ctggcgcagg aagtggatag
     5341 ccgcctgtcc acgcgcatcg aagggatctg tatcaagcat cggatgggca agaactcggt
     5401 caagatgtac gacaagtttg gtcgcgtcct gcgcatcgaa accaccacca acgatgtttc
     5461 cttcttcaag caccaccgcc aggtggaaca ccgggatggc caggccaccc gggaactggc
     5521 gaccgtcaag aagtcgatct acagtctgat cgatctgcgc gagattctcc tcggctgtaa
     5581 ccgccgctat ctcgaattcc tctccgcgct cgatgacttc tccgccggca gtcgccgcct
     5641 cgacaaactc accgctgcca aaacggtcga cggtcatcgc gtcaagggct tcaacttctt
     5701 cgacaagact gagcacacct tgctctgctc cctgcaacgc ccgcaattca acatcagtgg
     5761 cgtgcgccgt gccgatctca aacccttctt gcccaacctc tccgtggcca gtatcacccg
     5821 ctacctgaaa cgtctccgtg tcttgggcct catcaagaaa atcgctcacg cttaccgcta
     5881 ttacctgacc cgacttggcc gcaacgccat cgccgccgcc tgccatctca ccgaacaaat
     5941 catcgtccca gcactcgccg catgacaaaa tctgctcaga gtttgtgaag agttgagtca
     6001 taagggacta aatacgtaca gtaacgaaat ttgtactcga tgccatgtgg ggaattcccg
     6061 aagggagcag ggcgtttggc aagtgccaca cgccctgcgg gaagtttagt ggaaatgcac
     6121 ctcgtcatgt ccttcgactg gcgccagcag attggccccc aggacacgcg acatcaaagt
     6181 gacaaa
//
LOCUS       DAORPM010000141         6174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3427_length_6174_cov_7.824971, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000141 DAORPM010000000
VERSION     DAORPM010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3427_length_6174_cov_7.824971"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..299
                     /locus_tag="PLD53_07815"
     CDS             84..299
                     /locus_tag="PLD53_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60857.1"
                     /translation="MKKSRWLPEIGSVVTYIGRASKLARGAFNVRVVAETSADRMVVE
                     AIGRSGSPVRFTVKRENLAPLQPGLFD"
     gene            complement(362..646)
                     /locus_tag="PLD53_07820"
     CDS             complement(362..646)
                     /locus_tag="PLD53_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRC60858.1"
                     /translation="MIECIDGSIYTGIATDVAARYAAHASGKGARYTRAHPPTRLLVS
                     VEYPDRSSALKAEYRIKQLKPAAKRNFVTTMAMPTEELGASDNPGETTQP"
     gene            complement(732..1631)
                     /locus_tag="PLD53_07825"
     CDS             complement(732..1631)
                     /locus_tag="PLD53_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HRC60859.1"
                     /translation="MWFRNLQLYRMPKNWAISVTQLEEQLSTLTLQPCNATDAQSIGW
                     ISPGGKGTLVHTVNRQWLLALGVEQKLLPAAVVRQFADEHAKAIEEHEGRRIGRKEMR
                     ELREAMTSELLPRAFIRRRSTFGWIDPLNGWLVIDAAAPAKADEFLEHLHKSVDGLPA
                     RLLKVTQSPSAAMTGWVADGQAPGGFTLDQDLELRSAENAAVRYVKHTLDGEEIRQHI
                     ATGKVVTRLALTWGERISFLLDEKLQIKRLGFLDILKEEADSQAENEEERFDLDFTLM
                     TGELARLLNDLLDALGGELEKPA"
     gene            complement(2311..2508)
                     /locus_tag="PLD53_07830"
     CDS             complement(2311..2508)
                     /locus_tag="PLD53_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60860.1"
                     /translation="MMNTRTEPDNRSANYGPAMQAGNTEKPFIVAGNDDYQQMVAEAA
                     FIEAQRRATELVLSGSGRHEF"
     gene            complement(2712..3359)
                     /locus_tag="PLD53_07835"
     CDS             complement(2712..3359)
                     /locus_tag="PLD53_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC60861.1"
                     /translation="MKIKIILVDDHTMVREALRVVLEQDNRIQVLAEAGDGETALRLA
                     DKLAPDVVVMDVAMPGQSGIETTRRLLAKHPGIKVLALSTYLDRRIVQQMLDVGALGY
                     IVKSAAGTELKEGIRRVAEGHIYLCSAVNSLVAINLQEHKSQLKKPDRPTLSRRELQV
                     ATLLAEGKSAPEIAVKLSISPGTVDVHRRNLMRKLEVHNVVELTKYAMRTGLIQP"
     gene            complement(3356..4897)
                     /locus_tag="PLD53_07840"
     CDS             complement(3356..4897)
                     /locus_tag="PLD53_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRC60862.1"
                     /translation="MIPCKLPSDTEAPPDADQAPQQAHEQRSNETLPLAHYRQLFDLA
                     PMGYCLLDAQGLIIEANLAAARLLDQTPETLVGQKLSGFIFRDDREDYKRIHNRLLES
                     CETQACELRLARNGGTPSWVRLEATAVAKSDQPRLLHVALTDIDLHKCNEEQLKLAAG
                     VFTHAREGIMITAADGTIVDVNESFSRITGYSRNEVVGQNPRLLSSGRHEKAFYAALW
                     RDLIEKGRWYGEIWNRRKNGGVYAELLSISALRNPDGRNRNYVALFADITERKHAEEQ
                     MLETKVNLEAKVNDRTKQLRAVAAQLMMSEERERRMLAQELHDNLGQLLAIIKIKLTS
                     LATGLPQPTVNNLVELVDQADQAVRTITLNLSPPILHTLGLRSAFEWLAEEMERRYQL
                     TVHLDSEGELKPIVDEVQAMLFRSARELLINVARHAETSEASLSFLCNESRLTLAVSD
                     NGRGFDPGPSSGDKNAQNGFGLSSINERVVNIGGEMEIDSSPGNGTTITLTIPISISA
                     KEHRP"
     gene            complement(4957..5676)
                     /gene="dnaQ"
                     /locus_tag="PLD53_07845"
     CDS             complement(4957..5676)
                     /gene="dnaQ"
                     /locus_tag="PLD53_07845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRC60863.1"
                     /translation="MRQIFLDTETTGLEHKLGHRIIEIGCVEMRNRRLTNHHFHRYLN
                     PDRDIDAGALAVHGISLEFLQDKPRFADIAVEFIDFVRGAELIIHNATFDLGFLNSEL
                     ALLDMAPIDTVCPEVNDTLRMAKDLHPGKKNNLNALCERYGVDNSNRTLHGALLDAEI
                     LAEVYLAMTRGQESLMMELASNDEVRVNSAEDIQTSGSRKALLILRASEEERSRHLQQ
                     LAAIQEESKGRCLWLSSEKTI"
     gene            complement(5732..6169)
                     /gene="rnhA"
                     /locus_tag="PLD53_07850"
     CDS             complement(5732..6169)
                     /gene="rnhA"
                     /locus_tag="PLD53_07850"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRC60864.1"
                     /translation="MYADGGCRGNPGPGGWGALLQTGDIEKELWGGEPDTTNNRMELT
                     AVIRALEALKRPAAVHVHTDSQYVQKGISQWIHNWKRNGWRTSDKKPVKNDDLWQELD
                     GLTKQHEIKWLWVKGHAGHPGNERADALANRGIDELLTKRGEP"
BASE COUNT         1197 a         1674 c         1817 g         1486 t
ORIGIN      
        1 gcacctaatt gttcagtggg cgaaggcgcc ggtttcaact cggtaggtcg gaacggtcca
       61 aggaaatggt tcgacgaaat ggtatgaaga aatcccggtg gctgcctgag attggttcgg
      121 ttgtaacgta catcggccgt gccagcaaac tggcacgcgg cgctttcaac gttcgtgttg
      181 tggcggaaac cagtgcggat cgtatggtgg tggaagccat tgggcgtagc ggctcaccgg
      241 tgagatttac ggtcaagcgt gaaaatctcg ctccgcttca gcccggcttg ttcgattagg
      301 cattccaggg aaatacggga aggcgcaaag tctgtctgtc cagcaaagct gtgagcgcca
      361 ctcagggctg agttgtttcc cctgggttat cagaagcacc gagttcttcc gtgggcatgg
      421 ccattgtggt gacaaagttg cgcttggccg cgggcttcag ttgtttgatc cggtactccg
      481 ccttcaatgc actggagcga tcggggtatt ccacgctgac gagcagtcgg gtcggcggat
      541 gagcgcgcgt gtaccgagcg cctttcccgc tggcatgcgc agcgtagcgt gccgcaacat
      601 cggtcgctat gccggtgtaa atgctgccgt cgatgcattc gatcatataa aggaaccacg
      661 ggccatgctg tagcgccctg gaattttcga tgcttgatga attatccggc attctctgat
      721 ggcgcaaggg ctcaggctgg tttttccagc tcgccaccca gcgcgtcaag caggtcgttc
      781 agtagacgcg caagctcgcc ggtcatcaac gtgaaatcca gatcaaaacg ttcttcttcg
      841 ttttcggcct gactgtcggc ttcttccttg agaatatcga ggaagccgag gcgcttgatc
      901 tgcaattttt cgtccagcaa aaaggaaatg cgctcacccc aggtcagcgc cagtcgcgta
      961 acgaccttgc cggtggcgat atgctgacgg atctcctcgc cgtccagggt gtgctttaca
     1021 tagcgcaccg cagcgttttc ggccgagcgc agttcgagat cctgatcgag cgtaaatcca
     1081 cccggcgctt gaccgtcggc gacccagcca gtcatcgcgg ccgatggcga ttgtgtgacc
     1141 ttgagtagcc tggccggcag gccatcgacc gatttgtgca gatgctccag aaactcgtcg
     1201 gctttggccg gtgccgcggc gtcgatgacc agccagccgt tgagcggatc gatccagcca
     1261 aacgtgcttc ggcggcgaat gaaggcacgc ggtagcagtt cgctggtcat cgcttcgcgg
     1321 agttcgcgca tttccttgcg gccgatgcgg cggccttcat gttcctcaat cgctttagca
     1381 tgctcatcgg caaactgcct gaccactgcg gcgggcaaca gtttctgctc gacgccgagg
     1441 gccagcaacc actggcgatt gacggtatgg accagtgtgc ctttgccacc gggcgatatc
     1501 cagccgatgc tttgcgcgtc agttgcattg cagggctgca atgtcagggt ggaaagctgt
     1561 tcttcaagtt gggtaacgct gattgcccag ttcttgggca ttcggtagag ctggagattt
     1621 ctgaaccaca tgggagagac tcgaaaaacc aggaattata gggctgaatg cggcagtgaa
     1681 tgactcgatt ccatctattt gtgcatctga ttttctgtga atgttgaacg aggcctgcgg
     1741 tgtggttcat aatgcgcgta tgaatgatcc agaaccgatt gatccgcgtc gccggattcg
     1801 cgagcttacc gccatccccg agcgggatcg caccgacgaa caatgggatg agcttaacga
     1861 gcttgagatc cgcacggcac caggaaaccg cgagtcagac cggaatcagg atcggcagtt
     1921 ggggaagcgg ccgggttctc cgatgccggt gcgacgcccc gatcgcaatc agggtgtcaa
     1981 tagcaatggt ccgaggccgg attcacggcc gcaagaggga aggtccgata gtcgcccttc
     2041 caaacggcag catcggcgat ccaagcgtcc ggtcggtacg cccggtaaca gctgacggcc
     2101 gatttttccg aagtgccaag aaagcagatg cttggaatta ctctggttcc ttgtgtctgt
     2161 tagcgcaccg ggtccatcca ttccattttg tacaagcaat gacggcatca aaaaaaaccg
     2221 gttagcgtcg ttacgctaac cggtcttgat agaaaccgtt acgggattga tgcggggtta
     2281 caccggacac cagtcccctt cagcaatcgg tcagaattcg tgccggccgg atccgctcaa
     2341 tacgagttcg gtggcgcgcc gctgagcttc gataaaggca gcttctgcga ccatttgctg
     2401 gtagtcgtca ttacccgcaa caataaacgg cttttccgta ttacccgctt gcatcgcagg
     2461 accatagttt gcggacctat tgtcaggttc ggtacgggta ttcatcatgt ttgttctcct
     2521 gaggtatggg gttattgggc aaattattga tggctggaag aagtgccaca acccttaagg
     2581 ggcatccgcc tttcgccatt actgtttatg caatatattt cccataccga gacatgggaa
     2641 tacccgtttc cccatatttt tcgatcagac aagcaaaatt tctgaatgct gctggcgcgt
     2701 ggcaacgtga ttcaaggctg gatcaggccg gtacgcatgg cgtatttggt cagttcaacc
     2761 acgttgtgca cctcgagttt tcgcatcaga ttacggcggt gaacgtcgac cgtacctgga
     2821 gaaatactca atttgacagc aatttccggc gcgctcttgc cctcggcaag cagggtcgca
     2881 acttgcaatt cgcgccgtga caatgtgggc ctgtcaggct tctttagctg tgatttatgt
     2941 tcctgaaggt tgatagccac cagtgagttg actgcggagc aaaggtagat gtgcccttcg
     3001 gcaaccctgc gaattccttc tttcaactcg gtgcccgccg cagatttgac gatgtacccc
     3061 aaagcaccga cgtcgagcat ctgctgtacg atgcgccggt cgaggtaggt tgacaaggcc
     3121 agtaccttga ttccagggtg tttggcgagc agacgtcgtg tcgtttcgat acccgactgt
     3181 cccggcattg cgacatccat gacgactacg tcaggcgcca gcttatccgc aagtctcaat
     3241 gccgtttctc cgtcaccggc ttcggcgaga acctgaatgc ggttgtcctg ttccagcaca
     3301 acgcgcaatg cttcacggac catcgtatgg tcatcgacga gaattatctt gatcttcatg
     3361 gacgatgttc cttggcgctg atggaaatcg ggatggtcag agtgatcgtg gtgccatttc
     3421 cagggctgct gtcgatttcc atttcgcctc caatattgac tacgcgttca ttgatcgaac
     3481 tcaggccaaa accattttgt gcgttcttgt cacccgacga agggccaggg tcgaagccgc
     3541 ggccattgtc gctcaccgcc agcgtcagtc ggctttcatt gcatagaaat gacaggctgg
     3601 cctcgctggt ctcggcatgc ctggcgacat tgatcaggag ttcgcgggca gagcggaaga
     3661 gcatggcctg gacctcatcg acaatgggct tgagttcacc ctcggagtcg aggtgcaccg
     3721 tcagctggta tctacgttcc atttcctcgg caagccattc gaatgccgac ctgaggccaa
     3781 gcgtgtgcag tattggcggg ctcaggttca gggtgatcgt gcgcacagcc tggtcggcct
     3841 ggtccacgag ttcgaccaga ttgttaactg tcggttgcgg caatcctgtg gcaagcgagg
     3901 tcagttttat cttgataatc gccaacaact ggccaagatt gtcatgcagc tcctgcgcca
     3961 gcatgcgccg ctcgcgttct tcgctcatca tcagttgcgc cgcaaccgcg cgtaactgct
     4021 tggttcgatc gtttactttg gcctcgaggt tcactttcgt ttcgagcatc tgctcttcgg
     4081 catgcttgcg ttcggtaatg tctgcaaaca gcgcgacgta attcctgttc ctgccgtctg
     4141 gattgcgcaa ggcgctgatc gacagcaact cggcatacac ccccccgttc ttgcgccggt
     4201 tccagatttc gccgtaccag cggcctttct cgatcaggtc gcgccatagt gcagcataga
     4261 acgccttttc atggcggccc gaactgagca ggcgtggatt ctggccgaca acctcatttc
     4321 ggctgtagcc ggtaatgcgg ctgaacgact cattgacgtc gacgatcgtg ccgtccgccg
     4381 ccgtgatcat gatgccttcg cgggcatgcg tgaacacccc ggcggccagc ttgagctgtt
     4441 cttcattgca cttgtgcagg tcgatgtcag tgagagcgac gtgaagtaag cgcggttgat
     4501 cgcttttcgc tacagcagtg gcttccagac gtacccagga tggcgttccg ccattcctcg
     4561 ccagtcgcag ctcgcaggcc tgcgtttcgc aggactcgag cagtcggtta tggatgcgct
     4621 tatagtcctc ccggtcgtcc ctgaagatga atccggacag cttctgcccg accagcgttt
     4681 cgggtgtttg atcaagcaat cgggcggcgg caaggttggc ctcaatgatc aggccctgcg
     4741 cgtccaggag gcagtagccc atcggcgcaa gatcaaaaag ttggcgatag tgcgccagcg
     4801 gcagagtttc gttcgaccgc tgctcatgag cctgctgtgg tgcttgatca gcgtccgggg
     4861 gggcttcggt atcggagggg agtttgcagg ggatcatggc ttctcactga caggcaaggc
     4921 cgggtggtac gaggccaggt tggccatcgc atggattcat attgtcttct cggaggagag
     4981 ccagaggcat cggcctttgc tttcttcctg aattgcggcg agctgttgca gatgccggga
     5041 gcgctcctcc tcgctggcgc gtagaatcaa taacgccttg cgggagccgc ttgtttggat
     5101 gtcctctgcg ctattgacgc gtacttcgtc gttgctggcg agttccatca tcaggctttc
     5161 ctgaccacgt gtcatcgcaa gatagacttc ggcaagaatc tcggcgtcga gcagggcgcc
     5221 atgcagggtg cggttcgagt tgtctacgcc gtagcgctca caaagtgcat tcaggttgtt
     5281 cttcttgccg ggatgcaaat ccttggccat acgcagggtg tcgtttacct cggggcaaac
     5341 ggtatcgatc ggtgccatat cgagcagggc gagttcggaa ttcagaaagc cgagatcgaa
     5401 tgtcgcattg tggataatca gctcggcgcc gcgtacaaag tcgataaatt cgacggcgat
     5461 atcggcaaag cggggtttgt cctgaaggaa ttcgaggctg atgccgtgca ccgcaagcgc
     5521 tcccgcgtcg atgtcgcggt ccgggttgag gtaacggtga aagtgatggt tggtcagtcg
     5581 ccggttacgc atttcaacac acccaatttc aataattcga tgtccgagtt tgtgctcaag
     5641 tccggtcgtt tcggtatcga ggaatatctg gcgcatgctt ggattgtccg gtcaagttct
     5701 atgttgtgga aggcggctca acagtctaat actatggctc tccgcgcttg gtcagcaact
     5761 cgtcgattcc gcgattggcg agcgcgtcgg cgcgttcgtt gccaggatgt ccggcatgcc
     5821 ctttgaccca aagccatttg atttcatgtt gtttggtgag gccgtcgagt tcctgccaga
     5881 ggtcatcgtt ttttaccggc tttttgtcgg atgtgcgcca gccgttgcgt ttccagttgt
     5941 gaatccactg actgatgcct ttttgcacgt attgcgagtc ggtgtgaaca tgaactgcgg
     6001 ccgggcgctt gagggcctcg agtgcgcgga tgacggcggt cagttccatg cggttgttgg
     6061 tcgtgtccgg ttcgccgccc cacagctcct tctcgatgtc acccgtctgt aggagagcgc
     6121 cccagccgcc cggccccggg ttgccacgac agccgccgtc ggcgtagatg gtga
//
LOCUS       DAORPM010000142         6165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3433_length_6165_cov_10.545990, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000142 DAORPM010000000
VERSION     DAORPM010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3433_length_6165_cov_10.545990"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1700
                     /locus_tag="PLD53_07855"
     CDS             195..1700
                     /locus_tag="PLD53_07855"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRC60865.1"
                     /translation="MNMNTIKELQHFIDGHLVYGTSPRWGDVFNPATGQLAARAPLAS
                     ADETGKAIAAAVAALPEWSDTPPLRRARVLFKFRELLERHLDDLTAIVTAEHGKVLDD
                     AKGEIIRGMEVVEFACGIPHLLKGEYSEQIGNGVDSWSMRQPVGVCAGITPFNFPVMV
                     PMWMFPVALACGNTFILKPSERDPSASLFIADLLAQAKLPDGVFNVVHGDKVAVDTLL
                     TDPRVAAISFVGSTPVAEYIYSTGTAHGKRVQALGGAKNHMVVMPDADLEQAVDALIG
                     AAYGSAGERCMAISVAVAVGDVGDELVATLCGRAHSLKIGPGTAPAMEMGPLVTRQHL
                     AKVRAYVDLGVEEGADLVVDGRNLRIAGHENGYFLGACLFDHVSPEMRIYREEIFGPV
                     LCVVRVPDFEAAVELINAHEYGNGVAVFTRNGNTAREFVRRIQVGMVGVNVPIPVPMA
                     FHSFGGWKRSLFGDHAVHGPDGVHFYTRLKTATGRWPEDTASCASFVMPTH"
     gene            complement(1810..3768)
                     /locus_tag="PLD53_07860"
     CDS             complement(1810..3768)
                     /locus_tag="PLD53_07860"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HRC60866.1"
                     /translation="MIPKNYPRVIIEAVQPQIDCGRFPIKRVVDEKVVVSADIYKESH
                     DKLAAVVKYRKHGAKVWQENPMVFVDNDRWQGEFEVAEIGPWEYTVEGYAERYLSWVD
                     EITKKNVPGAILTSELLEGLAILKTSAGLAKGEDKAQLNAQIATIEGMMADEDQEVAI
                     DQAICSTMQRLMAKYPDRSEGAECTPALQVYVIRPITRFAAWYEFFPRSQSTVYGQHG
                     TLQDSRRRLADIKQMGFDVIYLPPIHPIGHAFRKGKNNSLVANPDDVGSPWAIGNEHG
                     GHMALEPKLGTWSDWDQFVATCRELKIEIALDYVMNCSPDHPYVTKHPDWFYHRPDGS
                     IKYAENPPKKYQDVYPLNFGTEDREGLWKEMLKIFLFWIEKGVTTFRVDNPHTKPVSF
                     WEWVIAEVHKKHPQVVFLAEAFTKPKMMRLLAKAGFAQSYTYFTWRDSKQDLTDYVSE
                     LTAGPMKDYFTGNFFANTPDILPPILQIGGRPAFIMRAVLAATLSTVYGIYSGYELCE
                     NEPIPGKEEYLDSEKYEIKQRDWNAPGNIVAVITRINKIRRERPALHNYNDVAFLAAD
                     NPQIIAYYKMTADRSDIVICVVNLDPFGKQSSFIHLPLEKMGIREDEQFQAHDMLSDE
                     RYLWSGRTAYVELSPSGKMAHIFKIYRW"
     gene            complement(3836..5041)
                     /gene="glgA"
                     /locus_tag="PLD53_07865"
     CDS             complement(3836..5041)
                     /gene="glgA"
                     /locus_tag="PLD53_07865"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRC60867.1"
                     /translation="MKVMFLTREYPPHVYGGAGVHVEYLAAELAKMMHVEVRCFGDQD
                     TVKECVRVKGFPFGKGSFSHVDKKLSGALDTFETNLETLSEQVDADLVHVHTWYAHLG
                     GILAKILYGIPLVHTVHSLEPLRPWKREQLGCGYDMSSWIERTSLDLADAVIAVSEGT
                     KTDILEHFDIDPAKITVIYNGINTEQYQQTSDTNTLEKYGIDPEKPIVLFVGRITRQK
                     GIIHLVNAVKYINADAQVVLCAGAPDTPEILAEMETAVKQVRKAGFKYLIWIKEMLSK
                     EEVIQLYSHARVFCCPSIYEPFGIINLEAMACHTAVVASAVGGIKEVVIDGVTGCLVP
                     LEQLHVAPFEPVDPDVFSRDMAAAINKVLDDPALAESMASAGQKRAHDVFSWSSIAQQ
                     TKDLYRSLV"
     gene            5704..>6165
                     /locus_tag="PLD53_07870"
     CDS             5704..>6165
                     /locus_tag="PLD53_07870"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRC60868.1"
                     /translation="MSALSRFIFTLTAIFLLVLSHVVSGVEPEIVINIERNGDAFIVD
                     TKFDLPVPLRTAWEVLTDFDNMTGILSNLTSSKIVRRDGKILYVAQEGLARFGILSYA
                     FASEREIRLEPMKRILARQITGNAERFESELELTQSGSATRVHYRAEVVPDS"
BASE COUNT         1359 a         1731 c         1662 g         1413 t
ORIGIN      
        1 gctccagctt ttgatatcga gcgtcttctt tatccatccc aattcgacat cagcacaatc
       61 agaaagttca ttcttcattg tgccgaatca atagcatcgt tccgttgtcc tcgctgtcgt
      121 cgacatgaac acgattgaca cttcaaaggt tataaaccta ctataggcaa aattcaaaat
      181 caagcacaac gttcatgaat atgaatacca tcaaagagct gcagcatttc atcgatggac
      241 atctggttta cggaacaagt ccgcgatggg gcgacgtttt caatccggca accggtcaat
      301 tggctgcgcg ggcgccgctg gcaagcgccg acgagaccgg caaggcgatc gccgctgccg
      361 ttgccgcact gccggaatgg tcggatactc cgccattgag gcgcgcacgc gttctcttca
      421 aattccgcga actacttgag cgccatctcg acgatctcac ggccatcgtc accgccgagc
      481 atggcaaagt actcgacgat gccaagggcg aaatcattcg cgggatggag gtcgtcgagt
      541 ttgcctgcgg cattccacac ctgttgaaag gggaatattc cgaacagatc ggtaatggcg
      601 tcgatagctg gtcgatgcgt cagccggtcg gcgtctgtgc cggtattacg cccttcaact
      661 ttcctgtgat ggtgccgatg tggatgtttc ccgtcgctct ggcctgtggc aataccttta
      721 tcctcaagcc ttccgagcgt gacccgtcgg ccagcctgtt catcgcggat ctgctggctc
      781 aggccaaact gccggatggg gtgttcaacg ttgtccatgg cgacaaggtc gctgtcgata
      841 ccttgcttac cgatccacgg gtggctgcga tcagttttgt cggctcgacg ccggttgccg
      901 aatacatcta ttcgacgggc actgcacatg gaaagcgggt gcaggcgctc ggtggcgcca
      961 agaatcacat ggtggtgatg ccggacgcgg accttgagca agcggtcgat gcgctgatcg
     1021 gcgcggctta cggttctgcc ggtgaacgtt gcatggcgat ctcggtggcg gtagcggtgg
     1081 gcgatgtcgg cgacgaattg gtggcgacac tatgcgggcg agcccactcc ctaaaaatcg
     1141 gtcccggcac cgcgccagcc atggaaatgg gcccgctggt gacgcggcaa catcttgcca
     1201 aagtacgcgc ttatgtcgac ctgggcgtcg aagaaggtgc ggacctcgtg gtcgatgggc
     1261 gcaatctgcg gattgccggt cacgaaaacg gctattttct tggcgcctgc ctgttcgacc
     1321 atgtgtcgcc ggaaatgcgc atctatcgtg aagaaatctt cggcccggtg ctgtgtgttg
     1381 tgcgtgtacc cgatttcgag gcggcagtcg aactgatcaa tgcccacgaa tatggcaacg
     1441 gtgtagctgt ctttacccgt aacggcaata ccgccagaga gtttgtacgg cggattcagg
     1501 tcggcatggt cggcgtgaac gtacccatcc ccgtcccgat ggccttccat agcttcggcg
     1561 gctggaagcg ttcgttattc ggtgaccatg cagtccacgg tcccgatggg gttcatttct
     1621 acacccgcct caagacggcg accggtcgct ggccggagga tacggcaagc tgcgccagtt
     1681 tcgtgatgcc gacccactga cctggtgtta ctggattcca aacgcgagta tgtccgcagt
     1741 gcccgaatgc agaaccacaa ctaacccgtg aaactcgggc ttgactgagt caaacccggc
     1801 tcctgtctgc taccagcggt agatcttgaa gatgtgcgcc atcttgccac tgggagaaag
     1861 ttcaacataa gccgtacggc ccgaccacag atagcgttcg tcgctgagca tatcgtgcgc
     1921 ctggaactgc tcgtcttcgc gaatacccat cttttcgagc ggcagatgga tgaacgagga
     1981 ttgtttgccg aaaggatcga gattgacgac gcatataacg atgtccgacc tgtctgcggt
     2041 catcttgtag taggcgataa tctggggatt atccgccgca aggaacgcaa cgtcgttgta
     2101 gttgtgcaga gccgggcgct cgcgacgaat cttgttgatg cgcgtgatca ccgccacgat
     2161 atttccgggt gcattccagt cgcgctgctt gatctcgtac ttttccgaat caagatactc
     2221 ttccttgcct ggaatcggct cgttttcgca caactcgtag ccgctgtaaa tgccgtagac
     2281 agtggagagt gtcgccgcca aaaccgcgcg catgataaat gcgggacgac cgccgatctg
     2341 cagaatcggg ggcaggatat caggcgtgtt ggcgaagaaa ttgccggtga agtagtcttt
     2401 catcggcccc gcagtcagct cactgacata gtcagtcaaa tcctgctttg aatcgcgcca
     2461 ggtgaagtag gtgtacgact gggcgaaacc tgctttggcc agcaaacgca tcattttggg
     2521 tttggtaaag gcctcggcca gaaaaacgac ctgggggtgt ttcttatgca cctcggcaat
     2581 cacccactcc cagaaactga ctggcttggt gtgtggattg tcgacgcgga aagtggtgac
     2641 ccctttttcg atccagaaca ggaagatctt cagcatctcc ttccacaggc cttcacgatc
     2701 ctcggtaccg aaattcagcg ggtacacatc ctgatacttc ttgggtggat tctcagcgta
     2761 cttgatcgag ccgtcgggcc gatggtagaa ccagtccgga tgctttgtca catagggatg
     2821 gtccggcgag caattcatca catagtccag cgcgatctcg atcttgagtt cgcgacaggt
     2881 cgcgacgaac tgatcccaat cactccaggt gcccagcttg ggttccagcg ccatgtggcc
     2941 gccgtgttca ttgccgattg cccaggggct gccaacatcg tcaggattgg ctaccagact
     3001 gttgttcttg cctttgcgga aagcatggcc aatggggtga atcggtggaa gatagatgac
     3061 gtcgaagccc atctgcttga tatcggccaa gcggcgacgg ctatcctgca aggtgccgtg
     3121 ctggccgtaa acggtactct gcgaacgggg gaaaaactcg taccatgcag cgaaccgcgt
     3181 gattggccga atcacataaa cctgcagtgc aggcgtgcac tcagcgccct cggaacgatc
     3241 aggatatttc gccatcagcc gttgcatcgt cgaacaaatg gcctgatcga tagccacttc
     3301 ctgatcttca tcggccatca tgccttcgat ggtggcgatc tgggcattca actgtgcctt
     3361 gtcctcgcct ttcgccaatc cggcggaagt cttcaggatc gccagacctt ccagtaactc
     3421 gctggtcagg atggcgcccg gcacattctt cttggtgatc tcgtcaaccc acgaaagata
     3481 acgctcggcg taaccctcga ctgtatattc ccaaggacct atctcggcaa cttcaaactc
     3541 gccctgccat ctgtcgttgt ctacaaagac cattggattt tcctgccaca ccttcgctcc
     3601 atgcttgcgg tacttgacga cagccgccag cttgtcgtga ctctccttgt agatatcggc
     3661 gctaacgacg accttctcgt caaccacgcg cttgatggga aatcgaccgc aatcgatctg
     3721 cggctgaacg gcttcaataa tgactcgggg gtagttcttg ggaatcatgg gggaaaacct
     3781 ctctcgtggc gaccagacct attttctgcc aaggcccaat caaggggaca tgaaatcaga
     3841 ccagcgaccg atatagatcc ttcgtctgct gtgcaatgct cgaccagctg aatacgtcgt
     3901 gtgcccgctt ttggccagcg ctggccatgg attctgccag tgccggatcg tccagaacct
     3961 tgttgatcgc agcggccata tcacgcgaaa acacatcggg gtcgaccggc tcgaagggcg
     4021 caacgtgcaa ctgctccaac ggcaccaggc aaccggtaac gccgtcgatc actacttcct
     4081 taataccacc cacagcggac gccactacgg ccgtgtggca cgccatggct tcaaggttaa
     4141 taatcccgaa gggctcgtag attgacgggc agcagaatac tctcgcatgg gaatacaact
     4201 gaatcacttc ttccttgctc agcatttcct tgatccagat caggtacttg aaccccgcct
     4261 tgcgtacctg cttgaccgca gtttccatct cggcaaggat ctccggggta tccggcgccc
     4321 cggcgcagag aacgacctgt gcatccgcat tgatgtactt gacggcattg accaaatgga
     4381 tgatgccctt ctgacgcgtg atccggccaa cgaaaagaac gatcggcttc tcaggatcaa
     4441 tcccgtattt ttcaagcgta ttcgtatcgg acgtctgttg gtactgctcg gtattgatgc
     4501 cgttatagat taccgtaatc ttggcaggat cgatgtcgaa atgctccagg atatcggtct
     4561 tggtgccctc cgacacggcg atcaccgcat ctgcgagatc gagcgaagtg cgctcgatcc
     4621 acgacgacat gtcgtagcca cagccgagct gctcgcgctt ccatgggcgt aacggctcca
     4681 gtgagtgcac cgtgtggacc agcggaatgc catagaggat cttcgccagg atgccaccaa
     4741 ggtgcgcgta ccaagtgtgc acatgcacca ggtcggcatc gacttgctcg ctcaaggtct
     4801 caagattggt ctcgaaagtg tccagcgcgc cgctgagctt cttgtcgacg tgggaaaaac
     4861 tgccttttcc gaacgggaag cccttcaccc gcacgcattc cttcacggta tcctggtcac
     4921 cgaagcaacg gacttcgacg tgcatcattt tggcgagttc tgcggccaga tactcgacgt
     4981 gaacaccggc tccgccataa acgtgaggcg gatattcacg ggttaggaac ataactttca
     5041 tcggtaaacc ttcctttaat tgagttcggg ggttgccaag acgctcgggc ggcaatggct
     5101 accgcgctac cgcacacttc aaaggatagg catttcaacc gcacagcctc aagtatccta
     5161 gagctgcgca ttccggtcaa atactcattt tatgagtagg ggtaattgct tgaatcataa
     5221 gcaagtctct gaaggcaagc acaagcgctg gcgacgacag gtggcggtca atgagcgaat
     5281 ttcaagccgc ttctcgttac ccaaaaccga gttcactccc gcagatctgc tttccgcgaa
     5341 ggtcgcctcc agcacacccg gtttgaaccc attcgaaacg gcaagaaacg cagtgaaaaa
     5401 tctgagcttg caaattccaa ctgtcgcctc gcaacctaca agtatttacg aatagctgat
     5461 tccttccgtg tccggatttc aaatacactt gctccacaac ctgaacgctg catcattcca
     5521 actaacttaa ttggggaatt cagtcatgct cacagaaaaa aagaagagca agaacacgcc
     5581 tgcgccacat taattccgga cgggctcggc ctgtttcgcc ctttctgggc gagcacgagt
     5641 gatcgaagta tctcatcgtg actaggacag aaccagcctg actcaatcca gcccgactca
     5701 actatgtctg cactgtctcg tttcatcttc accctgacag cgatttttct gttggtcctg
     5761 agccatgtcg tgtcgggtgt cgagccggaa attgtgatca acatagagag aaatggcgat
     5821 gcctttattg tcgatacgaa gtttgatcta ccggtgccct tgcgcacggc ctgggaagtt
     5881 ctcaccgatt tcgacaatat gaccggaatt ctgagtaacc tgacgtcgag caagatcgtc
     5941 cggcgcgatg gcaaaatctt gtatgtcgcc caggaaggac tcgcacgatt cggcatcctg
     6001 tcctatgcat tcgcttctga acgagagatt cggcttgagc caatgaagcg aattcttgcc
     6061 aggcagatta ctggaaatgc ggagcgcttt gagagtgagc ttgaattgac gcaatccgga
     6121 tccgccacac gggtgcacta ccgcgccgag gtagtcccgg attcg
//
LOCUS       DAORPM010000143         6154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3440_length_6154_cov_7.861945, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000143 DAORPM010000000
VERSION     DAORPM010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_6154_cov_7.861945"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PLD53_07875"
     CDS             complement(<1..1306)
                     /locus_tag="PLD53_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRC60869.1"
                     /translation="MTERRVVGPFNRVEGDLEITLDIAAGRIDAAYVNSPLYRGFEQI
                     LQGKIPADALTLVPRICGICSVAQSSASALALADAAGIVPPTNGRLTANLTLATENLA
                     DHLTHFYLFFMPDFARADYAGRSWHAETVERFRATLGTAANEMLSARAHFMQMMGLLA
                     GKWPHTLAVQPGGSTRPLLSSDIFRLHRILRDFRHFLESVTFGGTLEEVANLASRREL
                     NAWQSGSGGGDLGRFLRIADDLDLWHLGRATDRFMSYGCYDEPAGRLFPAGLWDEQLL
                     PLDAAAISEDISHAWYVAADLPQHPSGGGTVPVADKTGAYSWCKAPRLAGQVVETGAL
                     ARQLVAGHPLALDMVQAEGGSVGARVVARLLELAIVIPAMERWLAEITLGERWCVPAT
                     LPDEATGAGLIEAARGSLGHWLSVKRGLIHSYQIIAPTTWNFS"
     gene            complement(1303..2295)
                     /locus_tag="PLD53_07880"
     CDS             complement(1303..2295)
                     /locus_tag="PLD53_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupU protein"
                     /protein_id="HRC60870.1"
                     /translation="MNLLWLQSGGCGGCTLSWLGYEHGPLFQVLADEGIELVWHPSVS
                     EAGVDEVRELITRCADGRVELDVLCIEGSLLRGPQGSGAYHRFGGGGQSMAEWVRQIA
                     GRVRHVVAVGSCSAFGGVTAAGVNPTDACGLQYDGELEGGLLGPHFRDPSGLPVINIS
                     GCPPHPDWVTETLAMLARGRLDARDLDDYGRPRFYADQLVHHGCPRNEYYEYKASAGK
                     HSDQGCLMEQLGCKGTQAHADCNQRLWNGNGSCLRGGFACIRCTGPGFEEPGHPFETT
                     PKIAGIPLGLPSDMPKAWFVALAALSKSATPARVRENAREDHQVRTPPVKRGRK"
     gene            complement(2292..3725)
                     /locus_tag="PLD53_07885"
     CDS             complement(2292..3725)
                     /locus_tag="PLD53_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRC60871.1"
                     /translation="MIKHAPLSVLVVDDEQRSLETLRRTLEEDFTVFTALSAEEGNAV
                     MGREFVHIVLSDQRMPGTSGVEFLRGVRDQWPDTVRLILSGYTEAEDIIAGINEAGIW
                     QYLMKPWHADQLLLTLQSAADLWRLQQENQRLSLELRDSPEYLARQVASRRVTAKAQA
                     GFDRLTRAADSPLNEVCELAKKIAGHDLSILITGDSGTGKELLARAIHYASPRAEGSF
                     VVENCSALPDTLLEGELFGHKRGAFTGATEDRIGLFQQADGGTLFLDEIGETSPAFQV
                     KLLRALQEGEVRPVGSPRPVAVDVRIIAATNRDLEDDVASGRFREDLYYRIAGMTLYL
                     PSLRERPQDIPLIVDELFKRSSATDLRMSADAMNLLVGYSWPGNVRELQNEVRRAIAL
                     GSGAVLGPELLSKRLTKPAKTPGKRMQTAEGGSGLLKHQLERLEEQVIRDAMLRHKGN
                     KTRVADELGLTRLGLRMKLTRLGLEDE"
     gene            complement(3722..5419)
                     /locus_tag="PLD53_07890"
     CDS             complement(3722..5419)
                     /locus_tag="PLD53_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protein"
                     /protein_id="HRC60872.1"
                     /translation="MRILLLTHAFNSLTQRLSSELQQRGHEISIEFDIADSVTEEAVA
                     LFRPDLIVAPYLRRAIPESVWSRHICLIVHPGIVGDRGPSALDWAIQGSAGEWGVTVL
                     QAEAQMDAGPVWASADFPMRAVKKSSIYRHEVTQAAVSAVLQAVECHMQGGFEPERPP
                     ESWWRPLMTQAERCIDWQRDDSATILCKIAASDGFPGVADSLFGQACHLFDAWPEAEL
                     RGSPGEVIGRRETALLRATVDGAVWIGHVKRSDSIKLPASLAFERESAMIPERPLEGW
                     WRASSPTWQDIAYEEAGNVGFLHFEFYNGAMSTGQCQRLRTALLWARQRPVKVLLLMG
                     GADFWSNGIHLNVIEAASLKEGSAADESWRNINAMNDLALELINTGQQLTVAALAGNT
                     GAGGCFLARAADFMWARQAVILNPHYKNMGNLYGSEYWTYLLPRRVGADAARAIMRDR
                     QPLTAQRAVEIGFADACLDGDLSAFRSEVARRAAELAADPEFAKKIDEKRATRLRDES
                     VKPLSAYREDELTHMRRNFYGFDPSYHIARHHFVRKSPASWTPRHLARHRDLGWSIP"
     gene            complement(5430..>6154)
                     /gene="hypE"
                     /locus_tag="PLD53_07895"
     CDS             complement(5430..>6154)
                     /gene="hypE"
                     /locus_tag="PLD53_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRC60873.1"
                     /translation="LEEGMPLSDLARIVESMANAARQAGVPVVTGDTKVVERGKGDGV
                     FITTTGVGVVPEGLQLGGNRARPGDAVLVSGSLGDHGVAIMSRRENLSFDAPIESDTA
                     ALHGLIAAMLAVRADLRCLRDPTRGGLAATLNEIAGQSGVGMMLDEKAIPVKPVVAAA
                     CEFLGLDPLYVANEGKLIAICAAADAERLLAAMRAHPLGQDAARIGEVIADDHHFVQL
                     TTAFGGRRIVDWLAGDQLPRIC"
BASE COUNT         1193 a         2002 c         1846 g         1113 t
ORIGIN      
        1 gcgaaaaatt ccaggtcgtc ggtgcgatga tctgatagct gtggatcagt ccgcgcttca
       61 cgctcaacca gtggccgagc gaaccgcggg cggcttcgat gaggccggct ccggtggcct
      121 catcgggcag cgtggcggga acgcaccacc gctcacccag ggtgatttcg gccagccagc
      181 gttccatagc cggaatcaca atggccaatt caagcaagcg tgccaccact cgcgcaccga
      241 cactgccgcc ttcagcctgc accatgtcca gcgcgagcgg gtgtccggcg accagttggc
      301 gcgccagcgc accggtctcg acaacctggc ccgccagtcg cggtgccttg caccaggagt
      361 acgcgcccgt cttgtcggct accggcaccg tacctccacc ggatggatgt tgcggtaaat
      421 cggccgcgac ataccacgca tgcgaaatat cttcgctgat tgcggcagca tcgagaggta
      481 aaagctgttc gtcccacaat ccggcaggaa agagtcggcc cgctggctcg tcgtagcagc
      541 cgtagctcat gaaccgatcg gtcgctcgtc ccagatgcca caaatcaaga tcgtcggcaa
      601 tgcgcaggaa acggcctaga tcgccaccac ctgaacccga ctgccatgca ttcaattcgc
      661 gccggctggc caagtttgcg acttcttcaa gtgtgccgcc aaaggtcacg ctttcaagga
      721 agtggcggaa atcgcgcagg atgcgatgca gacggaagat atccgagctc agtagcggac
      781 gagtcgatcc accgggctgt acggccaggg tatgcggcca tttcccggca agcaggccca
      841 tcatctgcat gaaatgcgcg cgtgccgaca gcatttcatt cgccgcggtt ccgagcgttg
      901 cgcgaaagcg ctcgacggtc tccgcatgcc acgacctgcc ggcatagtcg gcgcgcgcga
      961 aatccggcat gaaaaacagg tagaaatgcg tgagatggtc ggccagattt tcggtagcaa
     1021 gtgtcaggtt ggcggtcagc cggccattgg ttggcggaac gattcccgcc gcatcggcca
     1081 gcgccagcgc actggccgac gattgggcca ccgagcaaat gccgcaaatg cgcggtacca
     1141 gcgtcaaggc gtcggcaggt atcttgccct gcaaaatctg ctcgaaaccc cggtacagcg
     1201 gcgaattgac atacgcggcg tcgatccgtc cagcggcgat atcaagggtt atctcaagat
     1261 cgccttcgac ccgattgaag ggcccgacga cacggcgttc cgtcatttgc gccctcgctt
     1321 gactggcgga gtgcgcacct gatgatcttc cctggcgttt tcgcgcacac gcgccggcgt
     1381 cgctgatttc gacaatgccg cgagggccac aaaccacgcc ttcggcatgt cggagggtag
     1441 gcccagtggt atcccggcga tcttcggagt ggtttcaaag ggatggccag gctcctcgaa
     1501 accgggaccg gtgcagcgga tgcaggcaaa accgccgcgc agacaggagc cgttgccatt
     1561 ccatagccgc tggttgcaat cggcatgcgc ttgcgtcccc ttgcaaccca attgttccat
     1621 caggcagccc tggtccgagt gcttgcccgc gctggccttg tattcgtaat actcattgcg
     1681 cggacagccg tggtgcacca gttgatcggc gtagaaacgc ggacggccgt agtcgtcgag
     1741 atcgcgcgca tccagccggc cgcgtgcgag catcgccagc gtctcggtga cccagtccgg
     1801 atgcggcggg caaccggaaa tgttgatcac cggcagtccg gaggggtcgc gaaaatgagg
     1861 gccgagaagt ccgccttcga gttccccatc gtattgcagg ccgcaggcat cggtcggatt
     1921 gacgccggct gcggtgacgc cgccaaacgc cgaacaacta cccacggcga cgacatgccg
     1981 cacccgcccg gcaatctgtc tgacccactc ggccatcgac tgcccaccac caccgaagcg
     2041 gtggtaggcg ccgctccctt gcggcccgcg tagcagggag ccttcgatac acagaacatc
     2101 aagctcaacc ctgccgtccg cgcagcgcgt gatcagttcc cgcacttcat cgacgccggc
     2161 ttccgaaaca ctcggatgcc agaccagctc gatgccctcg tcggccagca cctgaaataa
     2221 agggccgtgt tcgtaaccga gccaggacag cgtgcagccg ccacaaccgc cggattgcag
     2281 ccagagcaga ttcattcgtc ctccagtcca agacgcgtca gcttcatgcg caagccgagc
     2341 cgggtaagcc ccagttcgtc cgccacacgc gtcttgtttc ccttgtggcg cagcatggcg
     2401 tcacggatga cctgttcctc caggcgttcc agttgatgct tgagcaagcc ggagccgccc
     2461 tcggccgtct gcatccgctt tccgggtgtc ttcgccggtt tcgtcagccg ctttgacagc
     2521 agttcgggcc caagaacggc gccgctgccc agtgcaatgg cacgacgcac ctcgttttgc
     2581 agttcccgaa cgttgccggg ccacgaataa ccgacgagaa gattcatggc atcggcggac
     2641 atacgcaaat ccgtcgccga cgatctcttg aacaactcgt cgacgatcag tggtatatcc
     2701 tgaggccgtt cgcgcaagga tggcaggtaa agcgtcatgc cggcaattcg ataatagaga
     2761 tcttcgcgaa aacggccgct ggcgacgtcg tcctcgaggt cgcgattggt ggcggcgatg
     2821 atgcgaacat cgacggcgac cgggcgcggg ctgccgaccg ggcgcacttc accctcctgc
     2881 aaggcgcgaa gcaacttgac ctggaaagcc ggcgaggtct cgccgatttc gtccaggaac
     2941 agcgttccgc catcggcctg ctgaaacagc ccgatgcggt cctcggtggc gcctgtaaac
     3001 gcgccgcgct tgtggccgaa aagctcgcct tcgagcagcg tgtcgggcag cgcgctgcag
     3061 ttttccacga caaatgaacc ttcggcacgc ggactggcat agtgaatggc gcgtgccagc
     3121 agttccttgc cggttccgga atcaccggtg atcagtatcg acagatcgtg tccggcgatt
     3181 ttttttgcca gttcgcagac ttcattcagc ggactgtcgg cggcccgggt gagacggtcg
     3241 aaaccggcct gtgccttggc ggtgacgcgc cggctggcca cttggcgcgc caggtattcc
     3301 gggctgtcgc gcaattccag cgacagtcgc tgattctcct gttgcaggcg ccacagatcg
     3361 gccgcactct gcaaggtcag caataattgg tctgcatgcc agggcttcat caggtattgc
     3421 cagatgccgg cttcattgat gccggcgatg atgtcctcgg cttcggtata ccccgaaagg
     3481 atcagacgca ccgtgtccgg ccattgatcg cgcactccac gcaggaactc gaccccggag
     3541 gtgccgggca tgcgctggtc ggacaacacg atgtgcacaa actcgcgccc catcacggca
     3601 ttgccttcct cggcggacaa agcggtaaac acggtgaaat cttcttcgag cgtgcgccgc
     3661 agcgtctcca gggaacgctg ttcgtcgtcg accaccagga cggacaaggg agcgtgcttg
     3721 atcacggaat gctccagccc aggtcgcggt gacgcgccag atgccgcggc gtccaggacg
     3781 ccggcgattt gcgaacgaaa tgatgacggg cgatgtgata actcgggtcg aagccgtaaa
     3841 aattgcgccg catgtgcgtc aactcatcct cgcggtaagc agaaagcggt ttgaccgact
     3901 cgtcgcgaag gcgtgtcgca cgcttctcgt cgatcttttt ggcgaattct ggatcggcag
     3961 caagttcggc cgcgcgacgc gctacttcgc ttcgaaatgc ggacaaatca ccatccaggc
     4021 aggcgtcggc gaagccaatc tccacggcac gctgcgcggt cagcggttga cggtcacgca
     4081 tgatcgcacg cgccgcatcc gcgcccacac ggcgcggcag caggtaggtc cagtattccg
     4141 agccgtaaag attgcccatg ttcttgtaat gcggattcag gatcacggct tgtcgcgccc
     4201 acatgaaatc ggcggctcgc gccagaaagc agccgccggc cccggtgttg ccggcgagcg
     4261 ccgcgacggt aagttgttgt ccggtgttga tcagttccag cgccagatcg ttcatcgcat
     4321 tgatgttacg ccaggattcg tcggctgcgg aaccctcttt caggctggcc gcttcgatca
     4381 cgttgaggtg gatgccgttc gaccagaagt cggcgccgcc catgagcagc agaactttca
     4441 ccggccgctg cctagcccac agtaacgccg tgcgcagacg ttggcattgc ccggtggaca
     4501 tcgcgccgtt gtagaactcg aagtgcagga aaccgacgtt gccggcttct tcgtaggcga
     4561 tgtcttgcca ggtaggcgag ctggcccgcc accagccctc caagggtcgc tcgggaatca
     4621 tcgccgattc gcgttcgaaa gccagactcg cgggcagctt gatgctgtcg ctgcgcttga
     4681 catggccgat ccacacggca ccatcgaccg ttgcccgcag caaggccgtc tcccgccgcc
     4741 caatcacttc accgggcgag ccacggagtt cagcttcggg ccaggcgtca aacaaatggc
     4801 aggcttggcc aaacaggctg tcggcgactc cgggaaatcc atccgaagca gcgatcttgc
     4861 acaatatcgt cgcgctgtcg tcacgctgcc agtcgatgca tcgctcggcc tgcgtcatca
     4921 acggtcgcca ccagctttca ggtgggcgtt ccggttcaaa gccaccctgc atatggcact
     4981 cgaccgcttg cagcacagca ctgactgccg cctgcgtcac ctcgtgccga tagatgctcg
     5041 atttcttcac tgcccgcatc ggaaaatctg cgctcgccca aaccggcccg gcatccattt
     5101 gcgcctcggc ctgcaatacg gtgacgcccc actcgcccgc actcccctga atcgcccagt
     5161 ccagcgccga cggcccgcga tcaccaacga taccgggatg aacgatgagg cagatgtgtc
     5221 gcgaccagac ggattcagga atcgcgcgac gtaagtaggg cgcgacgatg agatcgggcc
     5281 gaaacagcgc cacggcttcc tcggtcactg aatcggcgat atcgaattcg atcgagatct
     5341 cgtgcccacg ctgctgcagc tcggagctta gtcgctgcgt cagactattg aaggcgtgcg
     5401 tgagcaacag gatgcgcatc gctcgctctt cagcagatac gcggcaactg atcaccggcc
     5461 agccagtcga caatccgccg gccgccaaaa gccgtggtca gttgtacgaa atgatggtcg
     5521 tcggcaatga cctcaccgat ccgcgcagcg tcctgcccca gcggatgcgc gcgcatcgcg
     5581 gcgagcaggc gctcggcatc ggccgccgcg caaatcgcga tcagtttgcc ctcgttggcc
     5641 acgtagagcg gatcgagacc gaggaattcg caggccgcgg cgaccaccgg cttcaccgga
     5701 atcgctttct cgtcgagcat catgccgaca cccgactggc cggcgatttc gttgagcgtc
     5761 gccgccagtc cgccacgcgt cggatcgcgc aaacagcgca ggtcggcgcg gacggcgagc
     5821 atggctgcaa tcagtccatg cagcgcggcg gtatcggatt cgatcggtgc gtcgaaggaa
     5881 agattttcgc gcctgctcat gatcgccaca ccgtgatcgc ccagcgatcc ggaaaccagc
     5941 accgcatcgc ccggccgcgc tcgattgccg ccgagttgta agccttcagg cacgacgccg
     6001 acgccggtag tggtaatgaa caccccgtcg cccttgcccc gctcgaccac tttggtatct
     6061 cccgtcacta ccggcacgcc ggcctgacgc gcggcattag ccatcgattc gacgatgcgc
     6121 gccagatcgc tcaacggcat gccctcttcg agga
//
LOCUS       DAORPM010000144         6051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3542_length_6051_cov_13.165777, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000144 DAORPM010000000
VERSION     DAORPM010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3542_length_6051_cov_13.165777"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLD53_07900"
     CDS             complement(<1..673)
                     /locus_tag="PLD53_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRC60874.1"
                     /translation="MMNFSGARAHKFAESPVLKLRLPAWRSRLMALLILTCFATLIGR
                     SFYLQVLNNDFLKEKGESRYLRDLEISASRGSIADRHGDVLAISTPMKSVWAMPADAR
                     LSQEQTKLLARLLEMDIKELSRKLASDKSFVFLKRQLPPDVAEQVAAQKLPGIGQDKE
                     YRRFYPSGEMTAHIVGFTGIDDKGLEGVELAFQGQLLGHPGSRRVIKDRRGQIVEDVG
                     SIKLPQ"
     gene            complement(670..945)
                     /gene="ftsL"
                     /locus_tag="PLD53_07905"
     CDS             complement(670..945)
                     /gene="ftsL"
                     /locus_tag="PLD53_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRC60875.1"
                     /translation="MVRLNILLLLAVVICALSVVTSQHKARKLFQSLESEQARTRQLD
                     VEYGQLQLEMQTWATSPRIEKIARDKLKMRTPEAAKIITASPKGISQ"
     gene            complement(947..1912)
                     /gene="rsmH"
                     /locus_tag="PLD53_07910"
     CDS             complement(947..1912)
                     /gene="rsmH"
                     /locus_tag="PLD53_07910"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRC60876.1"
                     /translation="MSSALPHQTVLLQEAVEALDVKPSGTYIDGTFGYGGHSRAILER
                     LGPSGRLLALDRDPKAVAANEIDDQRLTLAHHHFGELAVVVRQAGIDAVDGVLLDIGV
                     SSPQLDEGGRGFSFRFDAPLDMRMDTTQGETAASFLALAGIKEITEVIRNYGEERFAF
                     QIAKKIVAARGERPVATTSELAALVREAVRTREPGQDPATRTFQALRIHVNQELDQLA
                     LVLPQAMDILKCGGRLVVICFHSLEDRIVKRFMRDLASPDRLPKNLPLRSADLPSPRL
                     RLVGKPVKAGAAEIATNPRARSAVMRVAEKLIGNTVGINLAGGRA"
     gene            complement(1909..2355)
                     /gene="mraZ"
                     /locus_tag="PLD53_07915"
     CDS             complement(1909..2355)
                     /gene="mraZ"
                     /locus_tag="PLD53_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRC60877.1"
                     /translation="MFQGATALNLDVKGRLAIPARHREALVAASEGRLVLTAHLHHCL
                     LLYPATAWEPIRDQLLAVSSFNKQSAAIQRVMVGNARDEVLDSAGRLLIAPELRRFAQ
                     LEKQVWLVGLGSHFEIWSDAGWQQQLDVTMDTADQPLPPGLEDLAL"
     gene            2803..3654
                     /locus_tag="PLD53_07920"
     CDS             2803..3654
                     /locus_tag="PLD53_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRC60878.1"
                     /translation="MTATDPTERKPSEKGAAENNISQLQAVIFDVDGTLAETELAGHR
                     VAFNRAFKEFGLDWHWSAALYGELLAVTGGKERIRLYVETYANQMLGRADLDAWVASL
                     HQRKSEIYSELVLSGAIPLRPGVARLIQELRTAGIRIAIATTTTPAGLRSLIMANFKD
                     DMDMLFEVIGAGDIVAKKKPAPDIYQWVLDRLNLPPEACLAVEDSYPGLTAARAAGIP
                     TLVTVNAYTANQKFEGALSVVSDLGEPNVPARHIEGLPLARKCVDVAQLRTWHPLCSE
                     TSQSAVL"
     gene            3727..5106
                     /locus_tag="PLD53_07925"
     CDS             3727..5106
                     /locus_tag="PLD53_07925"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HRC60879.1"
                     /translation="MDQSNRYADLSLKEEDLIKGGKHILVAYKMKPKAGYGYLEAAAH
                     FAAESSTGTNVEVSTTDDFTKGVDGLVYHIDEATEDMRIAYPIDLFDRNMTDGRMMIV
                     SFLTLVIGNNQGMGDIEHGKIYDFYMPERAIQLFDGPSKDISDMWRILGRPIKDGGYI
                     AGTIIKPKLGLRPEPFAAAAYQFWLGGDFIKNDEPQGNQVFCPARKVYPLVYDAMKRA
                     MDETGEAKLFSANITADDHYEMLHRADFILETFGPDADKVAFLVDGFVGGPGMVTTAR
                     RQYPNQYLHYHRAGHGMITSPSAKRGYTAFVLGKISRLQGASGIHVGTMGYGKMEGDR
                     SDKIICYMLERDECQGPVYNQKWYGMKPTTSIISGGMNALRLPGFFENLGHGNVINTS
                     GGGAYGHVDSPAAGAISLRQSYECWKSGADPIEYAKEHKEFARAFESFPGDADKLFPG
                     WREKLGVHK"
     gene            5145..5948
                     /locus_tag="PLD53_07930"
     CDS             5145..5948
                     /locus_tag="PLD53_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HRC60880.1"
                     /translation="MTAKENYRIHKEPFYQAQGNEVVLFEAAYAARLPVMVKGPTGCG
                     KSRFVEYMAWKLGKPLITVACNEDMTASDLVGRYLLEANGTRWLDGPLTTAARIGAIC
                     YLDEIVEARQDTTVVIHPLTDHRRTLPIDKKGELVEAHQDFQLVISYNPGYQSLMKDL
                     KQSTKQRFTAFDFDYPEAALEAQIVATETGLDGESSAKLVKVGQAGRNLKGHGLDEGI
                     STRLLVYAATLIKGGVDARDACRMAMARPITDDVDIRTTLDHAIDATFT"
BASE COUNT         1369 a         1812 c         1651 g         1219 t
ORIGIN      
        1 cctgtggcag cttgatcgag cccacgtctt cgacgatctg gccgcgccgg tcctttatca
       61 cacggcgact tccgggatgt cccagcagtt gcccttgaaa ggccagttcg acaccctcaa
      121 gacccttgtc gtcaattccg gtgaatccga cgatatgcgc ggtcatctcg ccacttggat
      181 agaagcgacg atactccttg tcctgaccga taccgggcag tttctgtgct gccacctgct
      241 cggcgacatc gggaggcaac tggcgtttca agaacacgaa cgacttgtcg ctggccagct
      301 tgcgtgacag ttccttgatg tccatttcaa gtagcctggc aagcagtttg gtctgttcct
      361 gtgaaagtcg ggcatcggca ggcatcgccc aaaccgactt cattggcgtg gaaatcgcca
      421 gcacgtcacc gtggcgatcg gcgatactgc ctcgcgaggc ggaaatctca agatcccgca
      481 ggtagcgcga ttcacctttt tctttcaaaa aatcgttatt gagcacttgc agatagaacg
      541 atctgccaat caaggtggca aaacaggtca ggataagcag cgccatcaga cgcgaacgcc
      601 acgccggcaa acgcagcttc agcaccgggc tttcagcaaa cttgtgcgct cgggcaccac
      661 tgaaattcat cattgcgaaa tgcccttcgg cgacgcggta atgatttttg ccgcttcggg
      721 cgtacgcatc ttcaacttgt cgcgagcgat tttttcgatt cgcggcgagg tcgcccaggt
      781 ttgcatttcc agttgaagtt gcccgtattc gacgtcgagc tgcctggtgc gcgcctgttc
      841 gctttcgagc gactggaaga gcttgcgcgc cttgtgctgc gaggtcacta cgctgagtgc
      901 gcagatcacc acagccagaa gcaggaggat attcaggcga accatctcag gcacgccctc
      961 cagccagatt gattccgacc gtatttccaa tgagtttttc ggcaacccgc ataacggcgc
     1021 tacgcgcacg gggattggtc gcaatctcgg ccgctcccgc cttgaccggc ttacccacca
     1081 gacgcagcct gggcgacggc aggtcggctg aacgcaaggg aaggttcttg ggtaacctat
     1141 ccggcgaagc cagatcacgc atgaagcgct ttacgatgcg atcttcgagc gaatgaaaac
     1201 agatgaccac cagtcgaccg ccgcacttga gaatatccat tgcctgtggc aacactagcg
     1261 caagttgatc gagctcttga ttgacgtgaa tccgtaaagc ctgaaaggta cgcgtcgccg
     1321 gatcctggcc gggctcgcgg gtccgcacgg cctcgcgtac aagcgcggcg agctcgctcg
     1381 tggttgcaac aggccgctcg ccccgagcag ccacaatctt ctttgcaatc tggaaagcaa
     1441 accgttcttc gccatagttt ctaatgacct ccgtgatttc ctttattccg gcaagcgcca
     1501 gaaacgaagc tgccgtctcg ccctgcgtgg tatccattcg catgtcgagt ggcgcatcga
     1561 agcgaaagct gaagcctcgc cccccctcat cgagttgcgg cgacgacact ccaatatcca
     1621 gaagcacgcc atccacagca tcaatccctg cctgccgcac gaccacggca agctcgccga
     1681 aatggtggtg cgccaaggtc aatcgttgat cgtcgatttc attcgctgcc accgccttcg
     1741 ggtcgcgatc cagcgccagt agccgaccgc tcggcccaag ccgttcaaga atcgcccggc
     1801 tgtgcccgcc gtaaccgaag gtgccatcga tgtaagtacc cgagggtttt acatccaacg
     1861 cctcgactgc ctcctgcaac aacaccgtct ggtgcggcaa ggcagaactc acagcgccag
     1921 atcctcgagg cccggcggca agggctgatc agccgtgtcc atggtcacgt ccagctgttg
     1981 ctgccagccg gcatccgacc agatttcgaa atgactgccc agaccgacca gccagacctg
     2041 tttttcaagc tgggcgaatc gacgcagttc cggcgcaatc aggagacggc cagccgaatc
     2101 cagaacctcg tcgcgcgcat tgccgaccat cacgcgctgg atggccgcgg attgcttgtt
     2161 gaaactggat acggccagca gctggtcgcg aattggctcc caagccgtgg ccgggtagag
     2221 cagcaggcag tgatgcagat gggcggtcag caccagccgg ccctcggatg cagccacgag
     2281 cgcttcccga tggcgcgctg gaatggcgag ccgacccttg acatcgagat tcagcgcagt
     2341 tgcaccttga aacatcaaga ccggcccctc ctgacaactt gatgaaagac ttcccaattt
     2401 cccacttttt accactcgcc cccactttaa gacaaagatt taggcaaagc aagcggtttg
     2461 ttgcaatatt ttccaatgac tacaaagact tataaatcat ttctaagaaa ttctccctgc
     2521 cgtttttctt ttataaaaca acgataagaa aaaataactt aaagtggttt gttaccaaat
     2581 ggcgctgtac agccaccatt gaatccgaag tcccgtcgta caaagcaaac tctccccgtg
     2641 acgacaccct aggatcacgt tcgcactgcg ggaacccagc aagaccacgt atgatgcgtg
     2701 gatatatagt tagttattac ttgctataaa tagaaataag tttaactttt cataatgatc
     2761 ctctcccctt agactttgcg tatgaatgca aaggcacatc cagtgacggc tacagaccca
     2821 actgaacgga aaccatcgga aaaaggagcg gcagaaaaca acatttccca gttgcaggct
     2881 gtgatcttcg acgtagatgg gacgctggct gaaaccgagc ttgccggcca ccgcgtagcg
     2941 ttcaatcggg ctttcaagga attcggcctc gattggcatt ggtccgccgc gttgtatggc
     3001 gaattgctcg ccgttactgg tggcaaggag cgcattcgcc tctacgtcga aacctatgcc
     3061 aatcagatgc ttgggcgggc cgatttggac gcctgggtcg ccagcttgca tcagcgcaag
     3121 agcgagattt attcggagct ggtgctaagt ggcgcaattc cactccgccc tggcgttgcc
     3181 cggctgattc aggagctgcg tacggccggt attcgcatcg cgatcgccac gacgaccacg
     3241 ccagccggtc tgcgcagctt gatcatggcc aatttcaaag acgacatgga catgctcttt
     3301 gaagttatcg gcgccggcga catcgttgcc aagaaaaaac ccgcaccgga tatttaccaa
     3361 tgggtactag atcggctgaa cctgccaccg gaagcctgcc tggctgtcga ggattcctat
     3421 ccgggactga cggcggcgcg tgctgccggc ataccaacgc tggttacggt caacgcgtat
     3481 accgccaatc agaaattcga aggcgcgctc agcgtcgttt cggatctcgg cgaaccgaat
     3541 gtgcccgcac gccacattga aggtttgccg ctcgcccgaa aatgcgtcga tgtagctcag
     3601 ctgcgaacct ggcacccact ttgctcggag acatcgcaaa gcgcagtact atgactaatt
     3661 ggcaattggc cggactgtca ctcggcccac tcaaattttg caccattccc aaccacggag
     3721 aaaaccatgg accaatcgaa tcgttacgct gaccttagcc tgaaggaaga agacctcatc
     3781 aagggtggca agcacattct tgtcgcttac aagatgaagc ccaaggcagg gtacggctat
     3841 ctcgaagccg cggctcactt tgccgccgag tcatccacag gaaccaatgt cgaggtgtca
     3901 acgaccgacg atttcaccaa gggcgtggac gggctggtct atcacatcga cgaggccacc
     3961 gaggacatgc gcatcgcgta cccgatcgat ctgttcgacc gcaatatgac agacgggcga
     4021 atgatgattg tctccttcct gacgctggtg attggcaaca atcagggcat gggcgatatc
     4081 gagcatggca agatctatga cttctacatg cccgaacgcg ccattcagct gttcgacggg
     4141 ccgtccaagg acatctccga catgtggcgg atcctcggtc gcccgatcaa ggacggcggc
     4201 tacattgccg gcaccatcat caaacccaaa ctgggtctgc gccccgagcc gttcgcggcg
     4261 gcggcgtacc agttctggct gggcggcgat ttcatcaaga acgacgagcc gcagggcaac
     4321 caggtgttct gtccggccag aaaggtctat cccctggttt atgacgcgat gaagcgcgcc
     4381 atggacgaaa ccggcgaagc caagctgttc tccgccaaca tcaccgccga cgatcactac
     4441 gagatgctgc atcgcgccga cttcattctg gaaaccttcg gaccggacgc cgacaaggtg
     4501 gctttcctgg tcgatggctt cgtcggcgga cccggcatgg tcaccacggc ccgccgccaa
     4561 taccccaacc agtacctcca ctaccatcgc gccggacacg gcatgattac ctcgccttcg
     4621 gcaaagcgcg gctacacggc cttcgtcctg ggcaagattt cacgccttca aggggcatcc
     4681 ggcatacatg tgggcaccat gggttacggc aagatggagg gcgatcgctc cgataagatc
     4741 atctgctaca tgcttgagcg cgacgagtgc cagggcccgg tctacaacca gaaatggtat
     4801 ggcatgaagc cgaccacttc aattatttcc ggtggcatga atgcactgcg cctgcccggt
     4861 ttcttcgaga atctgggtca cggcaacgtg atcaatactt ccgggggtgg cgcctatggt
     4921 cacgtcgata gcccggctgc cggtgcgata tcgctgcgtc aatcgtacga gtgctggaaa
     4981 tccggtgctg atccgatcga atacgccaag gagcacaagg aatttgcccg cgcctttgaa
     5041 tcattccccg gcgacgctga caagttgttc cccggttggc gcgaaaaact cggtgtccat
     5101 aagtagtccg ttcggccttg ttaccgtatg cgaagaatga agccatgacc gccaaggaaa
     5161 actacaggat tcataaggag cctttttacc aggcacaagg caacgaagtc gtgctgttcg
     5221 aagctgccta tgccgcacgc ctgccggtga tggtgaaagg gccgaccggg tgtggcaaat
     5281 cgcgtttcgt cgaatacatg gcatggaagc tgggcaagcc gctgatcacc gtggcctgca
     5341 acgaggacat gacggcgagc gatctggtcg gccgctacct gctcgaagcc aacggaacac
     5401 ggtggctcga tggcccgctg acgacggcgg cgcggatcgg tgccatctgt tatctggatg
     5461 aaattgtcga ggcacgccag gacacgacgg tggtaatcca tccgctcacc gatcaccgcc
     5521 gcactttgcc gatcgacaaa aaaggcgagc tggtcgaagc gcatcaggac tttcaactgg
     5581 tgatctccta taaccccggc taccagagcc tgatgaaaga tctgaagcaa tcgacgaaac
     5641 agcgtttcac ggcattcgat ttcgattacc ccgaagccgc gcttgaggcg caaatcgttg
     5701 ccacggaaac cgggcttgac ggagagtcgt ccgccaagct ggtcaaagta ggtcaggcgg
     5761 gacgaaacct caagggccac ggcctcgacg agggcatttc gacccggctc ctggtctatg
     5821 ccgctacgtt gatcaaggga ggagtcgacg ccagggacgc ctgccggatg gccatggcgc
     5881 gcccgataac tgacgacgta gacatacgca ccacgctcga tcacgccatc gatgccacct
     5941 ttacctgagc gcccgatcca ggccggtcgg caacacaggc tcgcaacgta gcagcacatg
     6001 attaccggat tgcgacctct ggccgacgcc cgcaagcagg ccatgttcca c
//
LOCUS       DAORPM010000145         6038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3551_length_6038_cov_10.088417, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000145 DAORPM010000000
VERSION     DAORPM010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3551_length_6038_cov_10.088417"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..303)
                     /gene="rpsU"
                     /locus_tag="PLD53_07935"
     CDS             complement(91..303)
                     /gene="rpsU"
                     /locus_tag="PLD53_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRC60881.1"
                     /translation="MTAIRLKENEPFEVAIRRFKRTVEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRHHKRMRSQTLPPKLF"
     gene            437..1462
                     /gene="tsaD"
                     /locus_tag="PLD53_07940"
     CDS             437..1462
                     /gene="tsaD"
                     /locus_tag="PLD53_07940"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRC60882.1"
                     /translation="MLILGIESSCDETGIALYNTDAGGALLSHALHSQVLMHQEYGGV
                     VPELASRDHIRHVVPLLRKVLADSGRSLNELDAVAYTQGPGLAGALLVGAAFAEGLAA
                     SLGIPTVPVHHLEGHLLSPLLSSRPPQFPFIALLVSGGHSQLMQVNGVGEYQLLGETL
                     DDAAGEAFDKTAKMLGIPYPGGAELAELAESGHPDVVKLPRPMLRSGDLNFSFSGLKT
                     AVLTKVREIGAPSEQQRADIARGFQDAIVEVLVHKSLRAVTDSGLKRLVVAGGVGANR
                     ELRRRLDTQAANAAISVFYPALELCTDNGAMIALAGALRFQAGASIKPAGAFAVRPRW
                     PLAELTA"
     gene            complement(1451..2083)
                     /gene="plsY"
                     /locus_tag="PLD53_07945"
     CDS             complement(1451..2083)
                     /gene="plsY"
                     /locus_tag="PLD53_07945"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRC60883.1"
                     /translation="MSNVLIVLSAVVAAYLLGSIPFAVVSSRLFGLADPRSYGSKNPG
                     ATNVLRSGNKIAAATTLLGDLAKGWLAVFLARKYGADYGLKEAYVGLVALAVFFGHLY
                     PVFLKFKGGKGVATAAGVLLAIDPRLGLATLATWLIVLYALRYSSLAALVAAAAAPLF
                     AFFLWGSNVLLLTVCIIAMALIGKHWSNLQRLMAGAEPKIGAKKQLRSGR"
     gene            2219..2575
                     /locus_tag="PLD53_07950"
     CDS             2219..2575
                     /locus_tag="PLD53_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRC60884.1"
                     /translation="MDIIFIDDLRLSTLIGIYPREKAMPQTVEISLQIGVSTANAGTS
                     DDIGDTIDYAAVVDRLRTELAVRHFNLLEKLAEYVADLLLKDFGANWVRVSIAKLGMM
                     RGVRRVGVVIERGFPT"
     gene            complement(2578..3066)
                     /gene="ybaK"
                     /locus_tag="PLD53_07955"
     CDS             complement(2578..3066)
                     /gene="ybaK"
                     /locus_tag="PLD53_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRC60885.1"
                     /translation="MKSENAPDTPATKFLRQHGIAHSNHLYDYEEHGGTGVSSRELNV
                     DEHAVVKTLVMEDETAKPLIVLMHGDRKVSTKELARQIGCKKIEPCKPEVANRHTGFL
                     VGGTSPFGTRKKLPIYIQKSILDLPLIYINGGRRGFLVGVHPHDLMRALSPTVVDVAL
                     KG"
     gene            complement(3139..>6038)
                     /locus_tag="PLD53_07960"
     CDS             complement(3139..>6038)
                     /locus_tag="PLD53_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC60886.1"
                     /translation="RADGTLSIVCAILELDWVTQRFELQRVKPNGSITIIKDNGVTLF
                     RSPTIEGSIGRSFAGSPEFTDHLNTPGRGQYRIKGAFDGVSRLISHAHLARYPLIIAV
                     TASIDDALLAWRTELIKSIAFILFITVAAVLATRRYLYIDNESRTRLFKSERRFRSLL
                     ERAPDPTWIVDGSRIVECNDAAIEVLGYSSREDLLKLHPSKLSPPNQPDGENSRAKAR
                     RMTAIAKKQGLHRFEWILSKADASEFVAEMTFSVVDIEDRENIYCVCRDITERKRVES
                     LAERERVRLRTILETASDGIHILDSEGVLVEANEAFLEMLGYDDTVIGKLHVTDWDVQ
                     DSWEVISGRIEDMIVQRKKMVFETRHHRRDGSVLDVEISASGIEIEGKGYIYASSRDI
                     SERKRIEADLRIAATTFDSQEGIFITDANAGILRTNRAFEEITGYSSEEAVGQNPRLL
                     SSGRHPPAFYTAMWESIVSAGTWRGEIWNRRKNGESYPQYLSITAVKKEDGTVTHYVA
                     SFTDITERKVAADKIEHLAFYDSLTDLPNRRLMLDRLRQAVTNSARHNRHGALMLIDL
                     DNFKTLNDTLGHAIGDQLLKEVALRLKASIREGDTVARLGGDEFVVILEDLDESDAAP
                     RQAESVAEKILARLRYPYQLDLARADGSPGSRSHHCTSSIGISLFRDHSIQGDDLMKR
                     ADLAMYQAKAAGRNTIRFFDPGMQAAVTERVVMEADLRKAIADQQFLLHYQVQIDVSG
                     RAIGAEVLLRWQHPERGMVSPAEFIPLAEETELILPLGLWVLETACRQLSTWARTPGT
                     AHLTLAVNVSARQFRQPDFADQILAVLNDSKADPAKLKLELTESLLLDDVDRIIAKME
                     ILRAKGVGFSLDDFGTGYSSLAYLKRLPLSQLKIDQSFVRDVTTNPNAAAIARTVVAL
                     AQNLELGVIAEGVETEAQRDFLAQAGCFNYQGYFFGRPLPIEDFESLLKQV"
BASE COUNT         1226 a         1756 c         1778 g         1278 t
ORIGIN      
        1 gcatccggac atcaggcggc cacgaggcca gaaatgccaa aagccgccca tcgcaaaacg
       61 ggcggctgat cggcagactg caatccggta ttagaaaagc tttggcggca gggtctggct
      121 gcgcatgcgc ttgtgatggc gcttcactgc cgcggccaac ttgcgtttgc gctcggcagt
      181 gggtttttcg tagaactcgc gggcgcgcaa ttcggtcagc agaccggttt tttcaacggt
      241 gcgcttgaaa cggcgaatcg ctacttcgaa cggctcattt tccttcaggc gaatggcggt
      301 catcaactct atcctaattt caaaaatcga cagtttgcaa ctgagccgag cattataaac
      361 aaaggcgggc gcaaatacca agcgctttgt tcctgttttg tccggaggcg gtgggcgaca
      421 ggttagaatg ccgcccatgc tgattcttgg aattgaatcc tcctgcgacg agacaggtat
      481 tgcgctttac aacaccgatg ctggcggtgc gctactgtcg cacgcgctgc actctcaagt
      541 gctgatgcac caggaatacg gtggcgtggt tccggaactc gcctctcgag accatatccg
      601 gcatgtcgtg cctttgctgc gcaaggtatt ggccgatagc gggcgctcgc ttaacgagct
      661 tgatgccgtc gcttacacac agggccccgg actggcaggc gccttgttgg tcggcgccgc
      721 gtttgcggaa ggtctggccg cttcgctcgg cattccgacc gtaccggtac atcatcttga
      781 aggacatttg ctctcgccgc tgctttcgag ccggccgcca cagttcccct tcattgccct
      841 gcttgtctcc ggggggcaca gccagttgat gcaggtcaat ggtgtcggcg agtaccaatt
      901 gctcggtgaa acactggacg atgccgccgg cgaagccttc gacaagacag ccaaaatgct
      961 gggcattcct taccccggcg gtgcggagct ggccgagctg gctgagtctg gtcatccgga
     1021 cgtggtcaaa ctgccgcgcc cgatgttgcg ctcaggcgat ctgaacttca gtttcagcgg
     1081 tctgaaaacc gccgtgctga ccaaagtgcg cgaaatcggc gcgcccagcg agcaacaacg
     1141 tgccgacatt gcgcgcggct tccaggacgc cattgtcgaa gttctggtcc acaaatcgct
     1201 gcgcgcagtg acggacagtg gcctgaaacg gctggtcgtt gccggtggcg ttggtgccaa
     1261 ccgcgaactg cgccgacggc tcgatacgca ggcggcgaac gcggcgattt ccgtctttta
     1321 cccggcgctt gagttgtgca ccgacaacgg cgccatgatt gcgttggccg gcgctttgcg
     1381 ctttcaagcc ggggcttcga tcaagccagc cggcgcgttc gccgttcgac cgcgctggcc
     1441 gctggctgaa ttaacggcct gatcggagct gtttcttggc gccgatcttt ggctcggcac
     1501 ccgccatcag ccgctgcaga tttgaccagt gcttgccgat cagagccatt gcgatgatgc
     1561 agaccgtcag caacagcaca ttgctgcccc agaggaaaaa agcgaagagt ggtgcagcgg
     1621 ctgccgcaac cagcgctgca agggaagaat agcgcagtgc atacaagaca atgagccagg
     1681 tcgcaagcgt cgccagccca agccgtggat cgatggccag caacacgccg gccgccgtgg
     1741 cgacgccttt gccacccttg aatttcagga acaccgggta caggtgccca aagaagacgg
     1801 caagcgcgac caatccgaca taggcttctt tcaagccata gtcggcgccg tatttccggg
     1861 ccagaaaaac cgccagccag cccttggcca gatcgccgag cagcgttgtt gcagcggcaa
     1921 tcttgttgcc gctgcgcagc acattggtcg cccccgggtt cttagaaccg tagctgcgtg
     1981 ggtcagccag cccgaaaagc cggctggaaa ccacggcaaa cggaatggaa cccagaagat
     2041 aagcggcaac gacggcagac aagacgatca aaacgttgga catggacgaa ccccggaaac
     2101 tcgaagtgtg gaaagatggc tgggttagaa tgcgcacact acgcacactg ccggcctgtc
     2161 atctgactca gtccttccag gcggccaacg attttacacc gagcaggcac attcccagat
     2221 ggacatcatc ttcatcgacg acctacgact ctcgacgctg atcgggatct acccgcgcga
     2281 aaaggcgatg ccgcaaacgg tcgagatttc gttgcagatt ggcgtttcaa ctgccaacgc
     2341 tggcaccagt gacgatatcg gagacaccat tgactacgca gcggtcgtcg accgcctgcg
     2401 caccgagttg gcggttcgtc atttcaacct gctcgaaaaa ctcgccgaat atgttgccga
     2461 ccttttgctt aaagacttcg gcgcaaactg ggttcgcgtt tcgattgcca agcttggcat
     2521 gatgcgtggc gtgcggcgtg tcggtgtggt catcgagcgc ggcttcccca cctgatttca
     2581 gcctttcagc gccacatcga caacggttgg cgacagcgca cgcatcaagt cgtgcggatg
     2641 cacgccgacc aggaacccgc gccggcctcc gttgatgtag atcagcggca gatcgaggat
     2701 gctcttctga atgtatatcg gcaatttctt cctggtgccg aacgggctgg tcccgccaac
     2761 cagaaaaccg gtatgccggt ttgcgacctc cggcttgcag ggctcgatct tcttgcaacc
     2821 gatctgtctg gccagttcct tggtcgacac tttgcgatcg ccatgcatca agacaatcaa
     2881 gggcttcgcc gtctcgtctt ccatgaccag cgtcttgacc acggcgtgtt cgtcgacatt
     2941 gagctcgcgc gacgagacac ccgtgccgcc atgctcttca tagtcgtaca gatggttcga
     3001 gtgggcaatg ccgtgctggc gcagaaattt cgtcgctggt gtatcggggg cgttctcgga
     3061 tttcatgttg tttccgccaa agttatttct taagacagca tagtagaccg aggacggcag
     3121 tgtttcagca gccgagaact aaacctgttt caaaagcgac tcgaagtcct cgatgggcaa
     3181 ggggcggccg aagaaatagc cttgatagtt aaaacagccg gcttgagcca gaaagtcccg
     3241 ttgcgcttcc gtctcgacgc cttcggcgat gacgccgagt tcgagattct gggccaaagc
     3301 cacgacagtt ctggcgatcg cggcagcgtt cgggttggtc gttacgtcac ggacaaagga
     3361 ctggtcgatc ttcaattggc tcaagggcag gcgcttcagg taagcaagag aggaataacc
     3421 ggtgccgaag tcatccagcg aaaaaccgac gcctttggcc ctgagtattt ccatcttggc
     3481 gatgattctg tcgacgtcat cgagcagcag gctctcggtc agttccagct tcagctttgc
     3541 cggatcggct ttgctgtcgt tgagaacagc caggatttga tcggcgaaat ccggctgacg
     3601 gaactggcgg gcgctgacgt tcacggctag ggtcaggtgg gcggttcccg gcgttcttgc
     3661 ccaggtgctt aattgtcggc atgcggtctc aagaacccac aggccgagcg gcaagatcaa
     3721 ctctgtctct tcggccagcg gaatgaactc ggctggggat accatgcctc gctcgggatg
     3781 ttgccaacgc agcagtactt cggcgccgat tgcccggccg gatacgtcga tctgcacctg
     3841 gtagtgcaaa aggaactgct ggtcggcaat cgccttgcgc agatccgcct ccatgacaac
     3901 acgctcggtc accgccgcct gcatgccggg atcgaaaaaa cggatcgtgt tgcggccggc
     3961 ggccttggcc tgatacatcg ccaggtccgc acgtttcatc agatcatcgc cctggatcga
     4021 atgatcgcgg aacaggctga tgccgatgct cgaggtgcag tggtggctgc ggctgcccgg
     4081 cgagccgtcg gcccgagcca gatcgagctg atagggatag cgcaacctgg ccagaatttt
     4141 ctcagctacg ctttcagcct gcctcggggc ggcatcggac tcgtcgagat cttcaaggat
     4201 tacgacgaac tcgtcgccgc ccagacgcgc cacggtatcc ccctcgcgaa tgctggcttt
     4261 gagacgcaag gcgacctcct tcagaagctg gtcgccgatc gcatgaccca gggtgtcatt
     4321 gagcgtcttg aagttgtcga gatcgatcaa catcagtgcg ccgtgcctgt tgtgtcgcgc
     4381 actgttggtc acagcctgcc gcagacgatc aagcatcaag cgacgattgg gcagatcggt
     4441 cagcgagtcg tagaacgcca agtgctcgat cttgtcggca gccaccttgc gttcggtgat
     4501 gtcggtgaat gaagcgacgt aatgggtgac tgtcccgtct tccttcttca cggcggtaat
     4561 cgaaaggtac tgcggatagc tttcgccgtt cttgcgccgg ttccatatct cgccacgcca
     4621 tgttccggcc gaaacgatgc tttcccacat cgccgtatag aatgccgggg ggtggcggcc
     4681 tgaactgagc agacgcgggt tctggccgac cgcctcttcc gaggaatagc ctgtaatttc
     4741 ttcaaacgcg cgattggttc gcagtattcc ggcattggca tcggtaatga agataccttc
     4801 ctgggaatcg aaagtggttg ccgcgatgcg cagatcagct tcgatccgct tgcgctcgga
     4861 gatatcgcgc gaggatgcat agatataccc cttgccttca atttcgatgc ccgaggcgct
     4921 gatctcgacg tccagaacgc tcccgtcacg tcggtgatgg cgggtttcaa agaccatttt
     4981 cttgcgttgc acgatcatat cctcgatccg cccgctgatg acctcccacg aatcctgcac
     5041 atcccaatcg gtaacatgca gcttgccgat caccgtgtcg tcatacccga gcatttcaag
     5101 aaaggcttcg ttggcttcaa cgagcacgcc ttcgctatcc agaatgtgga tgccgtcgct
     5161 ggcggtttcc aggattgtcc gcagacggac acgctccctt tccgccagac tctcaacgcg
     5221 cttgcgctcg gtaatgtcgc gacagacaca gtagatgttc tccctgtcct caatgtcgac
     5281 gacggagaac gtcatctcgg caacaaattc cgaagcgtcg gccttgctga gaatccactc
     5341 gaaacgatgc aacccctgtt tcttggcgat cgccgtcatg cgtcgggcct tggccctgga
     5401 gttttcaccg tcgggttgat tgggtggtga gagctttgat gggtgaagct tcaacagatc
     5461 ttcgcgactg gagtagccaa gcacttcaat ggcggcgtca ttgcattcaa cgatgcgact
     5521 gccatcgacg atccaggtgg gatccggcgc gcgttccaag agcgagcgaa accggcgctc
     5581 gcttttgaac aggcgcgttc gtgactcgtt gtcgatgtaa aggtagcgcc gggttgccag
     5641 tacggccgca acagtgatga acaggatgaa tgcgatgctc ttgatcagct cggtgcgcca
     5701 ggccagcagc gcgtcgtcga tgctcgccgt cacggcgatg atcaacggat agcgggcgag
     5761 gtgggcgtga ctgatcagac ggctcacacc gtcgaaggcg cctttgatgc gatattgacc
     5821 acggccgggg gtattcagat ggtcagtgaa ctcagggctg ccggcaaagg atctgccgat
     5881 tgatccctcg atcgttggcg agcggaacaa ggtaaccccg ttgtccttga tgatcgtgat
     5941 cgagccgttc ggtttgaccc gctgcaactc gaaacgctgg gtaacccagt ccagctccag
     6001 aatggcgcaa acgatggaca aggtcccgtc cgctctgg
//
LOCUS       DAORPM010000146         5952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3620_length_5952_cov_16.652535, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000146 DAORPM010000000
VERSION     DAORPM010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3620_length_5952_cov_16.652535"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLD53_07965"
     CDS             <1..435
                     /locus_tag="PLD53_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRC60887.1"
                     /translation="ALSHYTDWTIGHVHSGALGWVAMVSIGTIYYLLPRLFGKSEMYS
                     VKLMTVHFWVATIGVVLYIASMWIAGVMQGLMWRSVNADGTLAYSFVESVKVSYPFWG
                     IRFIGGVLFLVGMLIMAYNMFKTMAGGSTEDAPVLVPAGQHA"
     gene            452..1066
                     /gene="ccoO"
                     /locus_tag="PLD53_07970"
     CDS             452..1066
                     /gene="ccoO"
                     /locus_tag="PLD53_07970"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRC60888.1"
                     /translation="MKFTHDAIERNVGLLVILTVLVISIGGLVEIVPLFFQKSTTTPV
                     EGLKPYDPVRLSGRDIYIREGCFLCHSQMIRPFRAETERYGPYSVAGEFVYDHPFQWG
                     SKRTGPDLQRVGGRYSDEWHRVHLINPRDVVPDSNMPAFAWLAEKPADAAGVSAKMSA
                     LRKVGVPYTDEDIAGASKQVEGKSEMDVLIAYLQGMGLERKGVK"
     gene            1071..1268
                     /locus_tag="PLD53_07975"
     CDS             1071..1268
                     /locus_tag="PLD53_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRC60889.1"
                     /translation="MDINDLRSVVTVVSMLSFLGIVWWAYGVKSNKQRFEEAANLPFT
                     DEETDRAELGLSSSSELRKTS"
     gene            1265..2197
                     /gene="ccoP"
                     /locus_tag="PLD53_07980"
     CDS             1265..2197
                     /gene="ccoP"
                     /locus_tag="PLD53_07980"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRC60890.1"
                     /translation="MSDFVNEFWNWYVILGVLVSILACGVVLWMQSIHHPSHEQTTDT
                     TGHVWDETLEEFNNPLPKWWMWLFYATVVFALIYAVLYPTLGGFQGVFGWSTAGEHQK
                     EVKSVEDKVKPLFDNYLKMDLKAVAADQKAMEMSGRLYQTYCMQCHGATAQGSRDQGF
                     PNLTDADWQWGGEPAQIVETITNGRMGMMPAYGGNPDAIGGEAGAKEVANYVRSLSGL
                     SNDSALSAKGKTKYETVCSACHGQDGKGMQALGAPNLTDKSWLYGSSEAKIVETITQG
                     RANQMPPWKDFLGEAKIHLLSAYILNLGAGANKQ"
     gene            2338..3756
                     /gene="ccoG"
                     /locus_tag="PLD53_07985"
     CDS             2338..3756
                     /gene="ccoG"
                     /locus_tag="PLD53_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRC60891.1"
                     /translation="MSKVVNNPDRPIVINSDGLYEKHKTVYARSVKGVFNNWRWVMVC
                     FTQALFYGLCWSDWDGRQAVLFHLVERKFYIFGMVFWPQDAIYLAILLVISAYALFLV
                     TAVGGRLFCGYACPQTVYTEIFMWIERKLEGERPARMKLDAQSMSARKFRVKFSKHFL
                     WLAIALWTGFTFVGYFTPIKELGHSVASFSLGPWESFWILFYAGFLYLMAGFMREQVC
                     KYMCPYARFQGVMFDPDTLIITYDVDRGEPRGRRRKNAGPETQQNLGDCVDCSICVQV
                     CPTGIDIRHGLQLECIACAACIDACDEVMDKVGSPRGLIRYSTENALAKRYKPRDILA
                     HLMRPRTILYSSILMTIILATIWNLTTRIPLKVDVLRDRSTLYREGDDGRIENVFTLQ
                     VKNTDVEAHRYMISVSGIDGIDIMGERIAEVPAASNKSILVVAAVREGVADKGSNKIS
                     FEVRSIANDNIAVQEKTTFFMP"
     gene            3753..4316
                     /locus_tag="PLD53_07990"
     CDS             3753..4316
                     /locus_tag="PLD53_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRC60892.1"
                     /translation="MNTKLNETVQPWYRSPWPWILMAGPVIVIVAGVITTYLAISSND
                     GLVDDDYYKQGLAVNQTTARIRNAISLGLQAELMQNAEGTRIRILLSGKPDTVLPDVL
                     KLHITHPTRAGFDQNVRLETDGSGFYSGVLSARLAGRWHIALEDEKREWRLTGEWIIE
                     GTESLRLPAELQGVTGPGMRTDTRRNE"
     gene            4430..5818
                     /locus_tag="PLD53_07995"
     CDS             4430..5818
                     /locus_tag="PLD53_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HRC60893.1"
                     /translation="MMGERQGGQERLFYAFNLEDHIPPGHLLRGIDQCLDLSELRHHL
                     ADFYSTTGRPSIDPELMIRMLIIGYSYGIRSERRLCEEVHLNLAYRWFCRLGIEEGVP
                     DHSTFSKNRHGRFRESGAFRWLFDEVVRNCMASGLVKGEGFAVDASLIAANASHQHSV
                     QVGEHCDWNRPEIDTRAMREYLAALDDEALAEVVPRKVSLSDPHSRWTAATGGIAFFA
                     YSTNYLIDTAHGIILDVEATPAHRTAEVDSTKVMIERIEARFDLKPERLIADTAYGTA
                     PMLAWMVDEKGIEPHVPVFDKTERKDGTFERDAFQWDEAADEYRCPAGQTLRRERRQY
                     KNPRHHITKANTIIYRAGQRDCAACPLKSRCCPNMTHRKIVRSVHERARDVARRIATT
                     PAYQRSRCERKKVEMLFAHLKSILKLDRLRLRGLNGANDEFMLAAIAQNLRRMSRLVT
                     HDPPIQGIGAPA"
BASE COUNT         1295 a         1528 c         1760 g         1369 t
ORIGIN      
        1 gccctgtcgc actacaccga ctggactatt ggtcacgtgc attcgggggc tctgggctgg
       61 gtcgcgatgg tgtcgatcgg gacgatctac tatcttctgc cgcgcctctt cggcaagtcg
      121 gaaatgtata gcgtcaagct gatgacggtg cacttctggg tggccaccat tggcgtcgtt
      181 ctctacattg cctcgatgtg gattgcaggc gtgatgcagg gccttatgtg gcggtctgtt
      241 aacgcggatg ggacgctggc gtacagtttt gtcgagtcgg tcaaggtgtc ctacccgttc
      301 tggggaatcc gtttcattgg cggtgtgctc tttctggtag gtatgttgat catggcctac
      361 aacatgttca agacgatggc cggtggtagc acggaagatg cacccgtgct ggttccagcc
      421 ggtcagcacg cctaacaggg aggctccaaa aatgaaattt actcatgacg cgatagaacg
      481 taacgtcggc ttgctggtca tcctgaccgt actggtgatc agtattggcg gcctggtaga
      541 aatagtgccg ctgttcttcc agaaatcgac aacgactccg gtcgagggtt tgaaacccta
      601 tgatccggtt cgattgtccg ggcgcgacat ctacatccgc gaaggctgtt tcctctgcca
      661 ctcgcagatg atccgcccgt ttcgcgccga gaccgagcgc tatgggcctt actcagtggc
      721 cggtgagttt gtctatgacc accccttcca gtggggttcc aagcggacag gcccggacct
      781 gcaacgggtt ggcggtcgat actcggatga gtggcatcgt gtgcacttga taaatccgcg
      841 tgacgtcgtt ccagactcca acatgccagc tttcgcctgg ctggccgaga aaccggcgga
      901 tgcggcaggc gtttcggcaa aaatgagtgc cctgcgcaag gtcggcgttc cctacaccga
      961 cgaagacatc gccggtgcca gtaagcaagt ggaaggcaag agcgaaatgg atgttctgat
     1021 tgcctacctg cagggcatgg ggctagaacg gaaaggggtc aagtaaagcc atggatatca
     1081 atgatctgcg gtctgtcgtg acggtggttt ccatgctgag ctttcttggg atcgtctggt
     1141 gggcttatgg cgtaaagagc aacaaacagc gatttgaaga agctgccaac ttgccgttta
     1201 cagatgagga aacggaccga gccgaacttg gcctttcttc cagtagcgaa ctgaggaaaa
     1261 catcatgagc gactttgtaa atgaattctg gaactggtat gtcatcctgg gggtgctggt
     1321 cagtatcctg gcgtgcggcg tcgttctctg gatgcaaagt attcaccatc cttcccacga
     1381 acaaacaacg gacaccaccg ggcacgtgtg ggacgagact ctggaagagt tcaacaatcc
     1441 gcttcccaag tggtggatgt ggctgttcta cgctaccgtt gttttcgcgc tgatttacgc
     1501 agtcttgtat ccgactctcg gtggttttca gggggtcttc ggttggtcga cggctggtga
     1561 gcaccagaag gaagtcaaga gcgtagagga caaggtcaag ccgctgttcg acaattacct
     1621 gaaaatggat ctgaaagccg tggctgcaga ccagaaggcc atggaaatga gtgggcgcct
     1681 ttatcagacc tattgcatgc agtgccacgg cgcgacggct cagggttcaa gagaccaagg
     1741 ctttcccaat cttaccgacg ctgattggca gtggggggga gagccggcac agattgtgga
     1801 gacgattacc aatgggcgta tgggtatgat gcctgcctat ggcggaaacc cggatgctat
     1861 cggtggcgag gctggcgcca aggaagtggc aaactacgtc cgctcgctct ccggactgag
     1921 caacgacagc gcgctttctg ccaagggcaa gacgaagtac gaaaccgtgt gcagtgcatg
     1981 ccacgggcag gacggcaaag gaatgcaagc cctgggcgcc cctaacttga ccgacaaatc
     2041 ctggctttac gggagttccg aggccaagat agtggaaacc atcacccagg ggcgcgccaa
     2101 ccagatgccg ccctggaaag atttcctcgg tgaggcaaag attcacttgc tctccgccta
     2161 catactcaat ctgggcgctg gggccaataa gcagtaagcg ttccggacac gggcaacgag
     2221 gttgctcgtg tctatttact gggcgggggt tgacggggac tctgtcacaa attgtttttc
     2281 aatcgtgttg tatattagag taacgtgaaa ttctgaaatt gtttggggct ggtagtcatg
     2341 agcaaggtgg ttaataaccc ggacaggccg atcgtcatca attcagacgg gctgtatgag
     2401 aagcacaaga cggtttacgc gcgtagtgta aaaggagttt tcaataattg gcgctgggtc
     2461 atggtttgct ttacccaagc cttgttttat ggcctgtgct ggagcgactg ggacggccgc
     2521 caagccgtcc tcttccacct tgtcgagcgg aagttttata ttttcggtat ggtgttctgg
     2581 cctcaggatg cgatttatct ggctatcctg ctggtcattt cggcctacgc gctgtttctc
     2641 gtgacggctg tcgggggtcg gctcttctgt ggttacgcct gtccgcagac ggtctatacg
     2701 gaaatattca tgtggatcga gcggaagctc gagggcgaac ggccggcgcg aatgaagctt
     2761 gacgcgcaat ccatgtcggc gcgcaagttc cgtgtgaaat tcagcaagca tttcctgtgg
     2821 ctggccattg ccttgtggac gggtttcacc tttgtcggct atttcacgcc gatcaaggag
     2881 ctcgggcatt ccgttgcctc gttttcactc ggcccgtggg aatctttctg gattcttttt
     2941 tacgccggtt tcctgtatct gatggccggc tttatgcgcg agcaggtgtg caagtatatg
     3001 tgtccgtatg ctcgtttcca gggggtaatg tttgacccgg atacgctgat tatcacttac
     3061 gatgtcgatc gtggcgaacc acgcggcagg agaaggaaga acgccgggcc agaaacacaa
     3121 caaaatctgg gcgactgtgt cgactgcagt atctgtgttc aggtttgccc gaccgggatc
     3181 gatattcgtc atgggttgca gcttgagtgc attgcttgtg ccgcgtgtat tgatgcctgt
     3241 gatgaagtaa tggacaaggt ggggtcgccg cgcggtctga ttcgctattc caccgagaac
     3301 gctctcgcca agcgctataa gccgcgcgat attctggccc atctgatgcg gccgcgcaca
     3361 attctgtact cgtcgattct gatgacgatt atcctcgcta cgatctggaa tctgaccacc
     3421 cgcatcccac tcaaggtcga tgttctgcgt gatcgctcaa cgctttaccg tgaaggagac
     3481 gacggacgga tcgagaatgt cttcacgctt caggtcaaga ataccgacgt tgaagcacac
     3541 cgctacatga tcagtgtcag tgggatcgat ggaattgaca tcatgggtga gcggatcgct
     3601 gaagtgccgg ctgcgtcgaa caagagcatt ctggttgttg cagcggtaag agaaggcgtc
     3661 gcagacaagg gttccaacaa gatcagtttt gaagttcgct caatagccaa tgacaatatt
     3721 gcggtccagg aaaaaacgac tttcttcatg ccatgaatac caaactgaat gaaacagtcc
     3781 aaccttggta tcggtctccc tggccgtgga tactcatggc ggggcctgtg atcgttatcg
     3841 ttgccggtgt gattaccact tacctggcga ttagcagcaa cgacggattg gtcgatgacg
     3901 actactacaa gcagggtctg gcggtgaatc agacaacggc tcgcattcgt aatgccatca
     3961 gtctgggttt gcaggcagag ttgatgcaaa acgcagaagg aacccggatt cgaattctcc
     4021 ttagcgggaa gcccgacacc gttctgccgg acgtgctgaa attgcatatt acccatccaa
     4081 ctcgcgcagg gtttgatcag aatgtgcgct tggagaccga tggttcggga ttctattcag
     4141 gcgttctcag tgcccgtctg gcaggccgtt ggcatatcgc cctggaagac gagaagcgcg
     4201 agtggcgttt gacaggtgag tggattatcg aaggaactga gtctttgcga ttgccggcgg
     4261 aactgcaagg agtaacgggg cccggaatgc ggactgacac ccggagaaat gagtagccgc
     4321 ttggggaatg tcgttgaaag gcagagcttg ggcgattctg ttgaaaaact cgccacgatt
     4381 catcgcgtgc gatacaatat gcgtatagca tttgtatcga ggtgcgaaga tgatgggaga
     4441 acggcaaggt ggtcaggaac gtttgttcta cgccttcaat cttgaagatc atattccgcc
     4501 agggcacctt ttacgtggaa tcgatcagtg tcttgacctg agcgaactgc gccaccacct
     4561 ggccgacttc tacagcacca ccggacgccc ctcgattgat cctgaattga tgatccggat
     4621 gctcatcatt ggctacagct atggcatccg ttctgaacgc cgtttgtgcg aggaagtaca
     4681 tttgaatctc gcctaccgct ggttctgtcg cctgggtata gaggagggtg tgccggatca
     4741 ctcgacgttc tccaagaatc ggcacgggcg atttcgagag agcggagcct ttcgctggct
     4801 atttgatgaa gttgttcgga actgcatggc ttcgggactg gtcaagggcg aaggattcgc
     4861 tgttgatgcc agcctgatcg cggcgaatgc aagccaccaa cacagcgttc aggtcggcga
     4921 gcattgcgat tggaaccgtc cggaaatcga cacgcgtgcc atgcgcgagt accttgccgc
     4981 ccttgatgac gaggcgcttg ccgaggttgt tccgcgcaag gtttcgctga gcgatccaca
     5041 ctcacgttgg acggcggcta ccggaggcat tgccttcttt gcttattcca ccaactatct
     5101 gatcgatacc gcccatggca tcatcctcga cgtggaggct acaccggcgc accgcacggc
     5161 agaagtggat tccaccaagg tcatgatcga gcgtatcgag gcgcgtttcg accttaagcc
     5221 cgagcgcctc attgcggata ccgcgtatgg aacggcgccg atgctggcct ggatggtcga
     5281 cgaaaagggc atcgagccgc atgttccggt cttcgacaag acagaacgca aagatggcac
     5341 ctttgagcgc gatgcgttcc agtgggatga ggcggccgac gaatatcgct gtccggccgg
     5401 tcaaacgcta cggcgcgaac ggcgccagta caagaatccg cgccatcaca tcacgaaggc
     5461 caacaccatc atctatcgtg cgggtcagcg tgattgcgcc gcctgccccc tgaaatcgcg
     5521 gtgttgcccg aacatgacgc atcgcaagat cgtgcgcagc gtccatgagc gtgcacgcga
     5581 cgtggctcgc cggatcgcaa cgacgccagc gtatcaacgc tcacgttgcg agcgcaagaa
     5641 ggttgagatg ctcttcgcgc acctcaagtc gatcttgaaa ttggatcgtc ttcggctacg
     5701 cggtctgaac ggtgccaacg acgaattcat gctcgcggcc atcgcgcaaa atctccggcg
     5761 catgtccaga ctcgtcaccc acgatccgcc cattcagggg atcggtgcgc ccgcgtaagg
     5821 cgcaaacgct tgaaatatcc aacaaaccgg gcattcaagc tcggatacgt ccccgagagg
     5881 gtgcaattcc ccgatccttg aacactacga agatgctgtc tcgaacacta cggggagttt
     5941 ttcaacagaa tc
//
LOCUS       DAORPM010000147         5952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3621_length_5952_cov_9.748855, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000147 DAORPM010000000
VERSION     DAORPM010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3621_length_5952_cov_9.748855"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PLD53_08000"
     CDS             complement(<1..914)
                     /locus_tag="PLD53_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRC60894.1"
                     /translation="MPSRHRALLILVALSGLALLSIALALMVGSIRVSPGEVWTSLVN
                     AGGGIAGDVVRSLRLPRALAGFACGGLLALAGALLQVLLRNPLADPYILGISGGAGVG
                     AMLAILFGLSAAGINGFAFLGALGAMVVVFGLAHGDGSWTQTRLLLTGVIVAAGCGAV
                     VALMLSIAPEHKLRGMLFWVMGDLSQTNDPVLALVILAAALLVSLPFARELNLLARGA
                     DTAQTLGVAVVRLRRGVFVVASLATAAAVTHAGSIGFVGLIVPHLVRLATGNDQRLLL
                     PASALAGGSLLVIADTLARAVVAPQQLPV"
     gene            complement(923..2785)
                     /locus_tag="PLD53_08005"
     CDS             complement(923..2785)
                     /locus_tag="PLD53_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC60895.1"
                     /translation="MYPRRAFTTVSIALLSAIPAIATGQDASQVSDPIVVTATRIPTR
                     ENELISDVSVIRREEIEQAAQSTLAELLGRQPGISFSSDGGPGSTQSLFIRGTNSDQA
                     VILVDGIRLNSATLGTTSLSRIPLSQIDHIEILRGPASSLYGADAIGGVIQIFTRQGG
                     GPAQFNGELAYGTYDTTRAVAGVSGSHEGFRYSFQYAQDRTDGFSNVHDSRSFAYNKD
                     LDGFKNESYTANLGYSFNADHEIGINAFASDGTNRYDGGFGASAAKDYKNKLNVSGTS
                     ITLRDRFLPAWQSTLRIGQGVDDAKYLTDGVTASSARTESNQFQWMNDIALPLGKLLA
                     GVEQLTQKVDASQTYTATKRTINSALLGWTASVGANRWQMNVRRDDISNADAKNTGTV
                     AYGYQFTDTLRASASAGTAYKAPSINALYFPYTPFVGQGNPNLKPETAKSAEIAMHWE
                     TASQKASATYYDNRIKDLILWTETPTGSYFYVPHNVSKARINGLTLAYTGYFGAWKLH
                     GSADFLNPEDESTGNQLARRAKQFGTVGLDYSGGHWSVGGEIVATGQRYSDAENTQRL
                     GGYTLFNLFGNYRLDKDFSLFVRANNVFDKYYEQVQYYQTPGANVLAGIRYQPR"
     regulatory      complement(2929..3147)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3308..3496
                     /locus_tag="PLD53_08010"
     CDS             3308..3496
                     /locus_tag="PLD53_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60896.1"
                     /translation="MLESKVVQTVSLCQELRAENDLLRQQLAATEEVRKGLSERMESA
                     RSRLEQLARQLPEAKATV"
     gene            3505..3849
                     /locus_tag="PLD53_08015"
     CDS             3505..3849
                     /locus_tag="PLD53_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRC60897.1"
                     /translation="MPNDANYLDVTLLGKEYRVACPPEEHDALLDAVAYVDEKMHDIA
                     EKTKSTISERIAVMAALNIAHEYLSQPQSPTETPPPDESETGLDITVVKRRISSMGAK
                     LEAVLAPQEKLL"
     gene            4109..4684
                     /locus_tag="PLD53_08020"
     CDS             4109..4684
                     /locus_tag="PLD53_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HRC60898.1"
                     /translation="MEAFLVSTGIVALAEIGDKTQLLAFILAARFRKPVPIILGILAA
                     TLTNHFLAGMLGSWITTLLGPETLRWVLGVSFIAMALWTLVPDEFEEKDAKLARFGVF
                     GTTLIAFFFAEMGDKTQVATVALAAQYRTVIEVVAGTTFGMMLSNVPAVLLGDRVAAR
                     MPARAVHGVAAAIFAVLGIATLLGAGERIGF"
     gene            4762..>5952
                     /locus_tag="PLD53_08025"
     CDS             4762..>5952
                     /locus_tag="PLD53_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HRC60899.1"
                     /translation="MLHFDNRLVRELPGESDTRNHSRQVHGAIWSPVMPTRVTAPELL
                     AYSREMAQLLGLDASDLASPQWVNALAGNALLPGMTAYATCYGGHQFGNWARQLGDGR
                     AIFLGEVVNGAGQRFELQLKGAGPTPYSRRADGRAVLRSSIREFLCSEAMHHLGVPTT
                     RALSLVTTGEAVVRDMFYDGNPTEEPGAVVCRVAPSFIRFGHFELPASRGDEALLRRL
                     VEFTITRDFPDFSADPDDGLIRWFAEICERTARLMVHWMRVGFVHGVMNTDNMSILGL
                     TIDYGPYGWVDDFDPSWTPNTTDASGRRYCFGRQPEIARWNLERLAEALAVIEPDHDG
                     LGLGLTRYDDIYNKEFQLAFAAKFGFTAWRDEDAALVEECFGLMHRAEVDMTIFFRGL
                     ARIDI"
BASE COUNT         1249 a         1685 c         1735 g         1283 t
ORIGIN      
        1 accggcagtt gctgcggcgc gacgacggca cgcgccagcg tatcggcaat gaccagcagg
       61 ctaccgccag cgagcgccga ggctggtagc agcaggcgtt gatcgttgcc ggtggcgaga
      121 cgcaccagat gtggaacgat caggccgacg aaaccgattg aaccggcgtg cgtgacggca
      181 gcggcggtgg ccagcgaggc cacgacaaac accccgcggc gcaagcgaac aacggccaca
      241 ccgagtgttt gcgccgtatc cgcgccccgc gccagcaggt tgagttcacg cgcgaaagga
      301 agcgagacga gcaatgcagc tgccagaatg acgagggcaa gaaccggatc gttggtttgc
      361 gaaagatcgc ccatcaccca gaacagcatc ccgcgcagct tgtgctcggg ggcgatggag
      421 agcatcagtg cgacaactgc gccacagccg gcggcaacga tgacaccggt aagcagcagg
      481 cgggtctgcg tccagcttcc gtcgccgtgt gccagaccga aaacgacgac catggcgcca
      541 agtgcgccga gaaaggcgaa gccgttgatg ccggcggcag acagaccgaa caggatggcc
      601 agcatcgcac ctacgccggc gcctccagaa attccaagta tgtaaggatc ggcaagcgga
      661 ttacgtagca ggacctggag caatgcgcca gccaacgcca gcaatccgcc acatgcaaaa
      721 ccggccagtg cgcgcggcaa acgaaggctg cgcaccacgt cgccggcgat tccgccgccc
      781 gcgttaacca gactcgtcca cacttcgccc gggcttacgc gaatgctacc caccatcaac
      841 gccagcgcaa tgctcaataa cgccagcccg gagagggcga cgagtatgag caaggcgcgg
      901 tggcggctgg gcatcgtcag aattatcggg gctgatatcg aattccggcc aacacgttgg
      961 cgccgggtgt ctggtaatac tgtacctgtt cgtagtattt atcgaacacg ttgttggcac
     1021 gaacgaacaa cgaaaaatcc ttgtcgagac gataattacc gaaaagatta aacagtgtat
     1081 agccgccaag gcgttgagtg ttctctgcat cgctgtagcg ctggcccgta gcgacaatct
     1141 ctccgccgac gctccagtgt ccaccgctat agtccagtcc cactgttccg aattgtttgg
     1201 cacgacgcgc caactggtta cctgtggatt catcttcagg gttgagaaag tcggcgctac
     1261 catgcagttt ccaagcacca aagtatcccg tatacgccag cgtcaaaccg ttgatgcgcg
     1321 ctttgctgac gttatgtgga acatagaaat acgaccctgt tggcgtctcg gtccacagaa
     1381 tcagatcctt tatccggttg tcgtaatagg tagccgaagc cttctggctt gcggtttccc
     1441 agtgcatggc gatttctgcg ctcttggctg tctcgggttt cagatttggg ttgccctgac
     1501 cgacaaaggg cgtatacggg aaatacaagg cattgattga cggcgccttg taggccgtac
     1561 cggcagaggc actcgctcgc agcgtatcgg tgaactggta gccataggcc acggtgccgg
     1621 tgttctttgc atctgcgttg gaaatatcat cacggcgtac gttcatctgc caacgatttg
     1681 caccgaccga tgccgtccaa ccgaggagcg cagaatttat cgtccgtttt gtcgcggtgt
     1741 aggtttgcga ggcatcgacc ttctgagtca gttgctcaac gcctgcgagc aacttgccaa
     1801 gtggcaaggc aatgtcattc atccactgga actggtttga ttcggtgcgt gccgagctcg
     1861 cagtcacgcc atcggtcaga tacttcgcgt cgtccacgcc ctggccgata cgcaatgtgc
     1921 tttgccaggc cggcaggaag cggtcacgca aggtgatgct cgtgccggaa acgttcagtt
     1981 tgttcttgta gtctttggca gcagacgccc cgaaaccgcc atcgtaacga ttggtgccat
     2041 cactcgcaaa cgcgttgatt ccgatttcat gatctgcgtt gaagctgtac ccgagattcg
     2101 ctgtatagct ttcgttcttg aaaccgtcga gatccttgtt gtacgcaaaa cttcgcgaat
     2161 cgtggacatt cgagaagcca tcggtcctgt cctgcgcata ttgaaagctg taacggaacc
     2221 cttcgtggct gccgctgacg cccgctacag cccgcgtggt gtcgtaggtg ccgtaggcaa
     2281 gttcgccatt gaattgtgcc gggccaccgc cttgccgggt aaaaatctgt atgactccac
     2341 caatcgcgtc cgcgccgtaa agcgatgatg ctggaccacg caaaatctcg atatggtcaa
     2401 tttgcgagag cggaatgcgc gacaatgatg tggtacccaa ggtggctgaa ttgagtcgga
     2461 tgccatcaac caggatcacc gcctgatctg aatttgtgcc gcgaataaag aggctttggg
     2521 tactgccggg gccgccatca ctggagaagc tgatacccgg ttggcgaccg agcaactcgg
     2581 ccaaggtcga ctgtgctgct tgctcgattt cttcccggcg gatgacgctg acgtcactaa
     2641 tcaactcgtt ttcccgtgtg ggaatccgtg ttgcggtgac cactatgggg tcgcttacct
     2701 gggaagcatc ctggcctgtg gcgattgccg gtatagcaga aagtagtgcg atagaaactg
     2761 ttgtgaaagc gcgacgtgga tacatttcgg atttctcccg atggccgatt gacgtccccg
     2821 caaaccggcg agcaataaat cggaactgcg cgatggagaa gtcttcgaga tgggcgcgtg
     2881 cttctgcaaa ctgcgctgag catgtcgtgt ccgcctcccc gcggcacttc cgtctgtttg
     2941 cgcgaaggcc ggtatccggg ctcacgagtc ttgatgtgtc gccttcccag gcgcttgccc
     3001 agtggcctat cgacacaacc tcactcgctt accgttgcgg gggcagcaca ggcatgatgt
     3061 cttgcagaca cgcacctgtt tcccgtttaa ccgctgcaga gaatttggaa aaccctgcgt
     3121 tgcggcacct tgcacgctat atttcgccct atgccagcaa cgaagggcgc gattatagcg
     3181 caaagcccga ttgagttgct caacgagttg agccgtgtga aataaatcac atttaaaacc
     3241 aaggcttaat ccttgacttc gcttggtttt taaaactata gtccattgca tgattgacga
     3301 actgaatatg ctcgaaagca aagttgtcca gactgtgtcg ctttgccaag agttgcgtgc
     3361 ggaaaacgac ctacttcggc agcaacttgc cgccactgag gaggtcagga agggcttgtc
     3421 cgaacgcatg gagtcggccc gcagccggct tgaacaactt gcccggcaat tgccggaagc
     3481 caaagccaca gtttgaacga acccatgccc aacgacgcca actatctcga cgttaccctg
     3541 ctcggcaagg aataccgtgt ggcctgcccg ccggaagagc acgatgccct gcttgatgcc
     3601 gtggcctatg ttgatgaaaa gatgcacgat attgctgaaa aaacgaagag tacaatttcc
     3661 gagcgcatcg ccgtcatggc ggcactgaac atcgctcacg aatacctttc tcagccgcaa
     3721 tcaccgaccg aaacgcctcc gcctgacgaa tcggaaacag gactggacat taccgtcgta
     3781 aagcgtagaa tttccagcat gggggcaaaa cttgaagcgg tgctcgcacc gcaggaaaag
     3841 ttgctctgag cctccgacag ttgttccctg cggtgtttgc caaggccata cattctttga
     3901 accaatgcta attggcgtcg gttgcagacc atagaagctg ctgttgtgcg cgccttactc
     3961 gatgggtgtg cctgatgcgg aaggaatgcg accaatctga actccggttc aggatgccgg
     4021 cctagcggca ctcgcgggga cataattcaa cagcgcgcga attaatttcg cgcgctgttt
     4081 ttttgtccag tcgcgaagaa cttgattgat ggaggcattt ctcgtatcaa ccggcattgt
     4141 tgccttggca gaaattggcg acaagaccca actgctggcc ttcatcctcg ctgcaagatt
     4201 ccgcaagccg gtcccaatca ttctgggcat cctggccgcg acgctgacca accatttctt
     4261 ggctggtatg ctgggttcct ggattaccac gctactcggt cccgaaacgc tgcgctgggt
     4321 gcttggggtt tccttcatcg ccatggcgct gtggacgctt gttcccgacg aatttgagga
     4381 aaaggacgcc aaactcgcgc gatttggcgt tttcggtacc acgttgattg cctttttctt
     4441 cgccgagatg ggtgacaaga cccaagtcgc gacagtcgcg ctggccgcac agtatcgaac
     4501 tgtcattgag gtggtggccg gcacaacctt cggcatgatg ctgtccaatg tgccggccgt
     4561 cctgctgggt gaccgcgtag cagccagaat gccggcacgt gccgtgcatg gcgtcgctgc
     4621 ggccattttt gccgtgctag gcattgccac cttgcttggt gcaggtgagc gtatcggttt
     4681 ctgaggagga gcacaggcgc aggaacttgt catgctgctt tcgtgcccaa ataaaacctt
     4741 cttcagcaac cggaatccgt gatgcttcat ttcgataacc gactggtccg cgaattgccg
     4801 ggcgagagtg acacacgcaa tcattcgcgc caggtgcacg gtgcgatctg gtcgccagtc
     4861 atgccaacgc gggttacagc gccggaattg ctggcctact ctcgcgaaat ggcgcaactt
     4921 ctcggccttg atgcgagtga tctcgcttca ccgcagtggg tgaacgccct ggccggcaac
     4981 gccctgctac cggggatgac ggcctacgcc acgtgctacg ggggccacca gttcggcaac
     5041 tgggcgcggc aactgggcga cggccgtgca atttttctcg gagaagtcgt caatggcgcc
     5101 gggcagcgat tcgagctgca gctcaagggt gccggcccga caccctattc gcgccgcgct
     5161 gacggccgtg ccgtgttgcg atcgtcgatc cgggaatttc tgtgcagcga agccatgcat
     5221 catctcggcg tcccgacgac ccgagcgctc tcgctggtaa cgacgggaga agcggtggtg
     5281 cgtgacatgt tctatgacgg caacccgacc gaagagccgg gcgcggtggt gtgccgcgtt
     5341 gcgccatcat ttatccgctt cgggcatttc gagctgcctg catcacgcgg cgacgaagcg
     5401 ttgctgcgcc ggttggtgga gttcacgatc acgcgcgatt tccctgattt ttctgccgac
     5461 ccggacgacg gcctgatacg ttggtttgcc gaaatatgcg agcgcaccgc gcgcctcatg
     5521 gtgcactgga tgcgggttgg ctttgttcat ggagtgatga ataccgacaa catgtcgatt
     5581 cttgggctga ccatcgatta tggtccttac ggttgggttg atgatttcga cccctcatgg
     5641 acgccgaaca cgacggatgc ctctggtcgt cgttattgtt ttggccggca acctgagatt
     5701 gcccgctgga acctcgaacg gcttgccgag gcattggccg ttattgagcc agaccatgac
     5761 ggactggggc tcgggctgac gcgctacgac gatatctaca acaaggaatt ccagctggcc
     5821 ttcgccgcca agttcggttt cacggcgtgg cgcgatgagg atgcggcctt ggttgaagaa
     5881 tgttttggcc tgatgcatcg ggccgaggtc gatatgacga tctttttccg cggcctggcg
     5941 cgaatcgata tt
//
LOCUS       DAORPM010000148         5947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3633_length_5947_cov_10.896809, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000148 DAORPM010000000
VERSION     DAORPM010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_5947_cov_10.896809"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="PLD53_08030"
     CDS             <1..2085
                     /locus_tag="PLD53_08030"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRC60900.1"
                     /translation="TGDLAKLVSQDAGLATRILSVANSPALRRGAELRSLDNCLTALG
                     TRLVRSIATCLSIQNLFDRQTGVSANELSGFWLHSLLVAELSRNLAGSTGYAHPDEAY
                     LGGLLHDVGELILLSALGEPYAQLLSTYRDEASLEAMETVRFATHHGEIGTWLADKWQ
                     LDSTFADGILFHHAKLEQITTAGTLPKLIWLAHTLVSTDEVTPEHDTQHQLLFGFASK
                     LSLATLRDQAEARTRQIAEALGLATPDKLVNIHVWANLKVTPLIEAPTNDAEGELSAM
                     ISGMALLQPLQQDLFALESDAEVLLSLRESARILFDLNRVAFLLCDQGENKLSAGSIG
                     GQPTIFRQVDVPLSGNGSLVTRAALQREICSSFDTGETAQVSLIDVQFARAFNSPGLL
                     CIPMIARKSVFGVMICGLSQSQHARLSRRLPWLMNFGRIAAISLETIQEARSYRANAE
                     QEASSLFSSRARKMVHEAGNPLGIIRSYLKILDRKLPEDTSVRQELEILTEEIDRVAT
                     IVGRMNEAPKQQAATSSQDVGNLINELMLLYREPLFDAKGIRVEIKQPGGELRVSCER
                     NSLKQILLNLWKNASEALNSGQQIRILLTDHVIHNGSVHIQVRLDDNGPGMSEAVMRS
                     IHRKPDITASSKRGLGLSIVGELASSQGISVTCRSELGKGTSIALLLPKFIAATQVIE
                     PTLNHLSSVEAK"
     gene            2082..>4228
                     /locus_tag="PLD53_08035"
     CDS             2082..>4228
                     /locus_tag="PLD53_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRC60901.1"
                     /translation="MNVASGIDTLTRFSRVAESQRILIVDDEPRYRSAYRELLAGAGR
                     IIEECGTGKEAILRLDRRDIDVVVLDLKLPDIDGTEIMAWLSRNGISTSIVVFSADDS
                     IDSAIDALRQGASEFIRKHSNPNDLIQSVDRALRRHRLEQEHAVMTATLEQSERLHRF
                     LVEQSPDLIYTLDERGHFIFVNSRVESLLGYRREDLIGQHYSLIVHDDDIEQARYAFH
                     ERRIGDRATTNFEVRLINKQNGVQHFDNRMVVAILSSQGIYMPEKNVSQQSFLGTSGF
                     ARDITERKRAEETINFQAFHDLLTGLPNRTLFMDRLELALPQAKRRNQRVGVMFLDLD
                     RFKLINDTYGHPEGDKLLQDFALRIRNCLRSGDTLARQGGDEFTVLLPDINNTEDVIM
                     IAQKMLAELKQPFSVAEQDYIATVSIGIAIYPEDGETSEDLIRNADLAMYQTKSQGKN
                     GYLEFTSAMNTTNGERLSLENDLRLALKKGDQFELYYQPQISLSKRCVVGVEALIRWH
                     HPTQGLISPDSFIPLAEETGLIVPLSDWVLNAGCIQLAKLRSMGFTNLKLGINLSPKE
                     FERGDLFERIATPLGTHALPPEMLEIEITESLLMKDAEAVITKVRQLRRAGIRISIDD
                     FGTRFSSLNYLRRFSVNSIKIDQSFIRDICATHGSSGIVHAIMGLARSFGLGVLAEGV
                     ENATQLSTLAGMGCDEMQGYHFSRPLPVDQLEN"
     assembly_gap    4229..4328
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4514..4657
                     /locus_tag="PLD53_08040"
     CDS             4514..4657
                     /locus_tag="PLD53_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60902.1"
                     /translation="MEKTRNKAKKFSSLDKLGQRILHIARSIAKERKDRAERAFERRM
                     MLN"
     gene            complement(4671..5915)
                     /locus_tag="PLD53_08045"
     CDS             complement(4671..5915)
                     /locus_tag="PLD53_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRC60903.1"
                     /translation="MWQVGALLVCLVLASLFANWWRRRHEEGAGRLSDASFRLAFPLT
                     GMVLVGIAMLVLQNFTKVNLFRLALPLLGSMALVRIVVFVLRQAFPTAAWLTTWERII
                     VALVWGWLALYITDLAPYVIDALEQVNFPIGKQRVDLWMILHGLVTVFLTVVFALWVA
                     GMIEARLMRFETVDSSLRMVGVRLAKSLLTVIAIFASLALVGIDMTALSVFTGALGVG
                     LGFGLKNIASNYISGFIILLDRSIRLGNVIQVGADGGMVTQITTRYTVLRHPGGTEFI
                     VPNDSLISGVVQNQTYSDSRLRLSTTVGVAYNCDLDLAMRLMVEAAATQLRVLADPGP
                     KVFLTLFADSSINLELGFWIADPEEGRGNVTSDINIAIWRAFREHGIEIPFPQRDVRV
                     VYEKSAVGPVSQESADQGKGVV"
BASE COUNT         1438 a         1669 c         1529 g         1211 t
ORIGIN      
        1 acgggcgact tagccaaact cgtttctcag gatgccggtc tggcgacccg aattctgagt
       61 gtcgccaact ctccggcact aagacggggc gccgagttgc gcagcctgga caactgcctt
      121 actgcacttg gcacacggct ggttcgttca attgcaacct gcctctcaat tcagaatctt
      181 tttgaccgcc agacaggcgt ttcggcaaat gaactgtcgg gtttctggct ccactcactg
      241 ttggtcgccg agctttcccg aaatctggcg ggttcgaccg gctatgcgca tccagatgaa
      301 gcctatctcg gcggactgtt gcacgatgtc ggcgaattga tcctgctctc cgcactcggt
      361 gagccctacg cgcaactgct atcaacttat cgtgatgaag cttcacttga ggcaatggaa
      421 acagtccgat ttgctaccca tcatggagaa atcggtactt ggctagccga taaatggcaa
      481 cttgattcga cctttgctga cggcattctg tttcaccatg ccaaactaga acaaataacc
      541 accgcaggca ccttacccaa actgatctgg ctagcccata cgctggtctc gacggatgag
      601 gttacgccgg agcacgacac ccaacatcag ctgttgtttg gtttcgctag caaactcagc
      661 cttgctacgt tgcgcgatca agcagaagca cgaacccgtc agatcgccga agcgcttggc
      721 ctggcaacac ccgacaaact ggtcaatatt catgtttggg caaatctgaa ggtaacaccg
      781 cttattgaag caccgaccaa cgatgcagag ggcgaactgt cggcaatgat tagcggaatg
      841 gcattattgc agccactaca gcaggatctt tttgccttgg aaagtgacgc tgaggtcctg
      901 ctttcgctgc gcgaatcggc cagaattctc ttcgacctga accgcgtggc cttcctgctt
      961 tgcgaccaag gtgaaaacaa gttatcggca ggatcaattg gtggacaacc gacaattttc
     1021 cggcaagtcg acgttcccct ctccggcaac ggatcattgg tcacccgcgc tgcattacaa
     1081 cgggaaattt gcagttcttt tgataccggc gaaacggcgc aggtttcatt gattgatgtt
     1141 cagtttgctc gcgccttcaa cagccccggt ctgctttgca ttccgatgat tgctcgcaag
     1201 agtgtctttg gcgtaatgat ttgtggcctt agccaatctc agcatgcccg actctcccgt
     1261 cgcctcccct ggttgatgaa ctttgggaga atcgccgcga tcagtctgga aactattcag
     1321 gaagcacgga gctatcgggc aaatgcagaa caggaagcat ccagtctatt ttccagccgg
     1381 gctcggaaga tggttcacga agcagggaat ccgctgggca ttatcagaag ctacctgaaa
     1441 atactggatc gaaagttgcc agaagacacg agcgtgcgtc aggagctgga aatactgacg
     1501 gaagaaatcg atcgtgtcgc aactattgtc ggacggatga atgaggcgcc aaaacaacag
     1561 gcagccacga gcagccagga cgtcggcaat ctaatcaacg aattgatgct tctctaccgt
     1621 gagcccttgt ttgacgcgaa gggcatccgg gtcgaaatca agcagcccgg cggggaactc
     1681 cgggtctctt gcgagcgaaa cagtctcaag caaattttgc tcaatctctg gaaaaacgcc
     1741 tctgaagcgc tgaacagcgg tcagcaaata aggatattgc tgaccgacca cgtcattcat
     1801 aacggcagcg tccacattca ggtgcgcctt gatgacaacg gtcccggcat gtccgaagcg
     1861 gtcatgcgat ccattcaccg caaaccggat attacggcgt ccagcaaacg tggcctgggc
     1921 ctgtcgattg tcggggaact cgccagcagt caaggcattt cggtgacctg ccgcagcgag
     1981 ttgggcaagg ggacaagcat tgcactgctc ttgccaaagt ttattgcagc gacgcaggtg
     2041 attgaaccga cgctcaatca cctgtcaagc gtggaggcaa aatgaacgtc gcctcgggga
     2101 ttgataccct gactcgcttt tcgcgagtcg cagaaagcca gcgcattctg atcgtggatg
     2161 acgagcctcg ataccgcagc gcctatcgcg aattgctcgc cggcgcgggc cggattatcg
     2221 aggagtgcgg caccggcaag gaagcgatct tgcgccttga tcgccgcgat attgatgtgg
     2281 ttgttctcga cctcaagttg cccgacattg acggcactga gatcatggcc tggctatcgc
     2341 gcaatggcat atcgacctct atcgtcgtct tcagtgccga cgactcgatc gactcggcga
     2401 ttgatgcctt gcgtcaggga gcctccgaat tcattcgcaa gcacagcaac cccaatgatc
     2461 tgatccagag cgtcgaccgc gcactacgcc gtcatcgact ggaacaggag catgcggtca
     2521 tgaccgcgac actggagcaa tcggagcgtt tgcaccgttt tctcgtcgaa cagtctcccg
     2581 atctgatcta cacgctggat gagcgcggtc acttcatttt cgtcaatagc cgggtcgaat
     2641 cgctgctcgg ctatcgccgt gaggacctga tcgggcaaca ctattcattg atcgtccatg
     2701 acgatgatat cgaacaggcg cgttacgcct tccatgagcg gcgaatcggc gatcgggcca
     2761 cgacaaattt cgaagtcagg ctcattaaca agcaaaacgg cgttcagcac tttgacaacc
     2821 ggatggtggt tgcaatcctg agttcacaag gcatttacat gccggaaaag aacgtctcgc
     2881 agcagagttt ccttggcaca tcgggctttg cacgggatat caccgaacgt aaacgtgccg
     2941 aagaaaccat caacttccag gcatttcacg accttctgac cggcctgccc aaccgcactt
     3001 tgttcatgga tcggcttgaa ctggcgcttc cccaggccaa gcggcgcaat cagcgggtcg
     3061 gtgtcatgtt cctcgacctt gatcgattca agctgattaa cgacacctat ggtcaccctg
     3121 agggtgacaa gttgctgcag gattttgccc tgcgaatccg caattgcctg cgttccggag
     3181 atacgctggc acgtcagggc ggcgatgaat tcacggtatt gctgcctgac atcaacaata
     3241 ccgaagacgt catcatgatt gcccagaaaa tgctggctga actcaagcag cccttctccg
     3301 tggcagagca ggactatatt gcgaccgtca gtatcggtat cgcgatttat cctgaagatg
     3361 gtgaaacctc ggaagacttg attcgcaatg ccgatctggc gatgtaccag accaagtctc
     3421 aaggcaagaa cggctacctt gagttcacct cggcaatgaa cacaaccaat ggcgagcgct
     3481 tgtcactgga gaacgatctg cgcctggcac tgaaaaaagg cgaccaattc gaactctact
     3541 atcagcccca gatcagtctc tccaaacgtt gcgtcgtcgg cgtcgaagcg ctgattcgct
     3601 ggcaccatcc gacgcagggg ctgatcagcc cggattcgtt tattcccctt gccgaagaaa
     3661 ccggtttgat cgtgccactc agcgactggg ttctcaacgc tggctgcatc cagcttgcca
     3721 agctgcgcag catgggcttt accaatctga aacttggcat caatctttcg cccaaagagt
     3781 ttgagcgtgg tgatctgttc gagcgtattg ccaccccgct tggcacccat gcgctgccac
     3841 cggagatgct tgagatcgaa atcaccgaaa gtctgttgat gaaggatgcc gaagctgtca
     3901 tcaccaaagt acggcaactt cgccgtgccg gcataagaat atcgattgac gattttggaa
     3961 cccgcttctc ctcactcaat tatctgcgcc gcttttcagt gaatagcatc aagatcgatc
     4021 aatcctttat ccgtgacatc tgcgccacgc acggctcaag cggaattgtg cacgcgatca
     4081 tgggtctggc ccgcagtttc ggcttgggcg tattggccga gggcgtcgag aatgccaccc
     4141 agctcagcac tctggccgga atgggctgcg atgaaatgca gggctaccat ttcagtcggc
     4201 cactgccagt ggatcaactg gaaaacttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnntg cgcacgctgg cagatggaga gccccgaaaa atcgacccat tacatctgca
     4381 ggcgcggctt gcgcacgctg caaaaagtaa attcgtcgtc gaaaatcgtt acaaacgaat
     4441 aacgaagcaa aacagacgcg cttgccccgc atcgctaaag ttaaatctcc tgtagttcac
     4501 caaggagaaa gtcatggaaa aaaccagaaa caaggcaaag aaattttcca gcctggacaa
     4561 gctcggccag cgcatccttc atatcgcacg ctctattgcc aaagaacgta aggatcgagc
     4621 tgaacgagct tttgaacgac gcatgatgct gaattagacc gcatcctgaa tcaaacaaca
     4681 ccctttccct gatcggcgga ttcctggctc actgggccaa ccgccgattt ttcgtaaacg
     4741 acccgcacgt cgcgctgcgg gaagggaatt tcaatgccat gctcacggaa agcccgccag
     4801 atggcgatat tgatatccga ggtgacattg ccacgccctt cttcgggatc ggcaatccag
     4861 aagccaagtt cgaggttgat actactgtcg gcaaataacg tcaggaaaac cttcgggcct
     4921 ggatcagcca gaacgcgcag ctgggtggcg gccgcctcaa ccatcaggcg catcgccaga
     4981 tcaagatcgc agttgtaggc aacccccacc gtggttgaca gacgcaggcg gctatccgaa
     5041 taggtctggt tctgcacgac gcccgagatc agtgagtcat tgggcacgat gaattcggtg
     5101 ccaccgggat ggcgcaacac cgtgtagcgc gtggtgatct gcgtcaccat gccaccgtcg
     5161 gcaccgacct gaatgacatt gccgagacga atcgagcgat caagcaaaat gatgaagccc
     5221 gagatgtagt tgctggcgat gttcttcaaa ccaaaaccca ggccgacacc gagcgcgccg
     5281 gtaaaaaccg agagcgcggt catgtcgatg ccgaccagcg ccaaactggc gaaaatggcg
     5341 atcaccgtca acagcgactt ggcaagccgc acgcccacca tgcgcaggct ggaatccacc
     5401 gtttcaaagc gcatcagccg cgcttcgatc atgccggcca cccagagcgc aaaaactacg
     5461 gtcaggaaga cggtgaccag accgtgcaaa atcatccaca aatcaacgcg ctgtttgccg
     5521 atcggaaaat taacctgctc cagcgcatca atcacataag gcgccagatc agtgatgtag
     5581 agcgccagcc agccccagac cagagcgacg ataatgcgct cccatgtcgt taaccaggca
     5641 gccgtcggaa aagcctgacg cagcacaaaa acaacgatgc gcaccagcgc catcgagccg
     5701 agcagcggca gggccaggcg gaacagatta accttggtga aattctgcaa aaccagcatc
     5761 gcaataccga ccaggaccat gccagtcagc ggaaaagcca ggcgaaaact ggcatcgctc
     5821 agccgccccg ccccttcttc atgtctcctc cgccaccagt tggcaaacag ggaggccaac
     5881 accagacaaa ccagcagcgc cccaacctgc caaatgaaat tggggtcgct tacgtcgtcc
     5941 cagagat
//
LOCUS       DAORPM010000149         5928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3658_length_5928_cov_14.393155, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000149 DAORPM010000000
VERSION     DAORPM010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3658_length_5928_cov_14.393155"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLD53_08050"
     CDS             <1..422
                     /locus_tag="PLD53_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235834.1"
                     /note="involved in the export of lipooligosaccharides
                     during nodulation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nodulation factor ABC transporter ATP-binding
                     protein NodI"
                     /protein_id="HRC60904.1"
                     /translation="GLDPQARHLIWDRLKQLTARGKTLILTTHFMDEAERLCDRLAVL
                     DNGHLVTEGAPRELIESHIEPQVVEVFGEAAATWARSRNTLGERLEIAGETAFFYCRN
                     TVPLLAALADEPGLRYIHRAASLEDVFIKLTGRELRD"
     gene            425..1228
                     /locus_tag="PLD53_08055"
     CDS             425..1228
                     /locus_tag="PLD53_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60905.1"
                     /translation="MGDAFNARPSIYSFPRPGLRWLPVWRRNFLVWRKLAIPSVLGNL
                     ADPLLYMLGLGYGLGGLLPQISGSSYVSFLAAGTVVASTMNAATFEALYSAFSRMHVQ
                     KTWDAILNTPLSLDHVLTGELVWAASKSLLSGLAILLVIAALGLTSSLLALWVIPVVF
                     LTGLAFAALGLIVCALAPSYDFFMYYFTLFITPMLLVSGVFFPADRLPAAVHAIASWL
                     PLSHAVQLARPLLQGEVPANVIGHIAALLTIATIAFGIATVLTRRRLLK"
     gene            1326..2543
                     /locus_tag="PLD53_08060"
     CDS             1326..2543
                     /locus_tag="PLD53_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC60906.1"
                     /translation="MARAAFATMFTAYSAVLPLVKLEWGMSAWQAGLIQSVHHAGFLI
                     SLFSVGFLTDRFGARRTYLAASFAAAISAALFAFIADDFLSALLLYGLTGLCSGGSYT
                     PGLAIISQQMTGRQQGQAMGFYLAAASLGYAFGLFIASAMIPLAGWRGSFVVTSLGPV
                     LGLLVGFWSLRNVPNVLHYRSGSRSIFKGLSRVIGNRAAMLSIWGYTFHAWELLGLWA
                     WLPAFLTAASIASGNSAQQAASVGATLTAITYLVAAGGNALGGALSDRHGRTLVALFM
                     GISSLVFSFSFGWMLGLPMWLLLLIATLYNATSVADSSILSTTLTELVDPASLGAAYS
                     LRSVLGFSAGAVAPWVFGVVLDAGRAAGGVSDTIVWALAWSTLGLGGLLGPLAIWRLR
                     QMPDARRLAGGKR"
     gene            2576..3139
                     /locus_tag="PLD53_08065"
     CDS             2576..3139
                     /locus_tag="PLD53_08065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRC60907.1"
                     /translation="MKTRLLAISGSSRQDSVNMRLLNTATDIARRQGAEVNVLDLRTL
                     ALPLYDGDLEAASGLPEGCLHLKQAIREHHALLVASPEYNGFFTPLLKNSIDWATRPQ
                     EGVPSPFPGKVAAIIAASPGALGGIRGLPALRILLSGIGVLVTPGQMSLPLAHKAFDE
                     KGDLINPQQQKVLEGVVGELLRTVHTD"
     gene            complement(3239..4861)
                     /locus_tag="PLD53_08070"
     CDS             complement(3239..4861)
                     /locus_tag="PLD53_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRC60908.1"
                     /translation="MDKPFAITLDPGSSLANHTGSWRTERPVYVDRLPPCNNQCPAGE
                     DIQGWLFHAESGNYEKAWQHLTKDNPFPAIMGRVCYHSCESVCNRAKIDSAVGINSVE
                     RFLGDEAIKRKWKLSQTAAETGKHVLIVGAGPSGMSAAYHLRRFGHRVTVYDAGPFMG
                     GMMRFGIPKYRLPRDVLDAEMQRVVDLGVEVKLNSKVDNLLETMESGKFDAAFLAVGA
                     HIGKRAFIPAGAVSKIVDAISVLRSMENEEKPMLGRRVIVYGGGNTAIDVARTAKRMG
                     AEPIIVYRRNREKAPAHDFEIEEAMQEGVMIKWLSTIKEAGDANITIEKMELDDTGFP
                     QPTGEFETLEADSVVLALGQDVDLGLLDGVPGLVVADGVVTVDPNMMTGHAGIFAGGD
                     MVPADRNITIAVGHGKKAARNIDAWLRGTSYIAPPKHAVANFESLNTWYYADAPKTVR
                     PMLDIIRRRSTFEEVQGGLDESTALFEARRCLSCGNCFECDNCYGVCPDNAVIKLGPG
                     NRFKFNLDYCKGCGMCVAECPCGAITMEPENI"
     gene            complement(4861..5874)
                     /locus_tag="PLD53_08075"
     CDS             complement(4861..5874)
                     /locus_tag="PLD53_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC60909.1"
                     /translation="MSLQPVKFYQTGTFTVGNRLLAPEERSVQANMERTNSLNSGHRA
                     CQGCGEALGARYAIDSAMIASNNQLIAANATGCLEVFSTPYPETSWQIPWIHSLFGNT
                     AAVATGIAAAIKVKQAKGRSGDVRVVAQGGDGGTTDIGFGCLSGMFERNDDVLYICYD
                     NGGYMNTGVQRSSATPPSARTATTMPVGPEPGNVFGQGKFVPAIAMAHEIPYVATATV
                     ADLRDLEYKVKKAMSFRGARYIQILVPCPLGWGAASQDTIRLSRLAKESGTFPVFEAE
                     YGELTGSSKIRRRVPVEEYLKPQKRFAHLFGKTPAVDTIARLQAMADKNIRRYELLDK
                     EAH"
BASE COUNT         1118 a         1791 c         1702 g         1317 t
ORIGIN      
        1 ccggcctcga tccacaagcg cgccacctga tctgggatcg tctgaagcaa ctcacggcac
       61 gcgggaagac tctgatcctg acgactcatt tcatggatga agccgagcga ctatgcgacc
      121 gtctggcggt gctcgacaac ggccatctgg tcaccgaagg cgcgccccgc gaattgatcg
      181 agtcgcacat cgaaccgcag gtggtagagg tattcggtga agccgcggcg acctgggcac
      241 gctcccggaa cacgttgggc gaacgcctcg agattgctgg cgaaaccgct ttcttctact
      301 gccgcaatac ggtgccgctg ctggctgctc tcgctgacga accgggcttg cgttacatcc
      361 accgtgccgc cagccttgaa gacgttttca tcaagctcac cgggcgagaa ctgagagact
      421 gagcatggga gacgctttca acgcaaggcc ctcgatctat tccttccccc gacccggcct
      481 gcgctggcta ccggtttggc ggcgaaactt tctcgtctgg cgcaagctgg cgataccttc
      541 ggttctcggc aacctcgccg acccgctgct ttacatgctc ggtctcggct acggacttgg
      601 cggcctgctg ccgcagatca gtggcagcag ttatgtcagc tttctagccg cggggacggt
      661 ggtcgccagc accatgaatg cagccacctt tgaagcgctt tactcggctt tctcgcgcat
      721 gcatgtgcag aagacctggg acgccattct caataccccg ctctcgctcg atcacgtcct
      781 gaccggcgaa ctggtctggg cagcaagcaa gagcctgctc tccggactag ccattctgtt
      841 ggtcatcgca gcgctggggt tgaccagttc actgctggcg ctgtgggtga ttccagtcgt
      901 tttcctcacc ggccttgcct tcgccgccct gggcctgatc gtctgcgcac tggcgccgtc
      961 ctacgatttc ttcatgtact acttcacact cttcattaca cccatgctac ttgtctccgg
     1021 agtcttcttt cctgccgacc ggttaccggc ggcagttcat gcaatcgcca gttggcttcc
     1081 tctttcgcat gcagtgcaac tcgcccgccc cttgttgcaa ggggaagtac cggccaatgt
     1141 gatcggacac attgccgcac tgctgaccat cgccacgatc gcctttggca tcgcaacggt
     1201 gctgacgcgt cggcgattgc ttaaatgaac ttcggccgca cgaagaacgt tgtgcacaaa
     1261 cccagtattt gtacgggtgt cgattgcagg ggctgcgctc aatgaaatgg ctaatgcagt
     1321 tttgtatggc acgtgcggcc ttcgccacga tgttcacggc ctattcggcg gtgttgcccc
     1381 tggtcaaact cgagtgggga atgtccgcat ggcaagcggg attgatccag tccgtccatc
     1441 acgctggttt tttgatatcg ctgttttcgg taggctttct tacggaccgc ttcggcgcac
     1501 gtcgaaccta tctcgccgcc agctttgccg ctgccatcag tgctgcgctg ttcgcattta
     1561 tcgccgacga tttcctttcc gcgctcttgc tctacgggct taccggactt tgctccggcg
     1621 gctcctacac gcccggactg gccatcatca gtcaacaaat gactggccgg cagcaagggc
     1681 aagcgatggg cttctatctt gccgccgcat ctctcggcta cgctttcggc ttgtttattg
     1741 ccagtgccat gattcccctc gcgggctggc gaggatcgtt tgtcgtcacg tcgctggggc
     1801 ctgtcctggg actgcttgtc ggattctggt cgctgcgcaa tgttcccaat gtgctgcatt
     1861 atcggtcggg cagtcgcagt attttcaagg gattgagccg cgttatcgga aatcgggcgg
     1921 caatgctgtc gatctggggc tataccttcc atgcttggga gttacttggt ttgtgggcgt
     1981 ggttaccggc tttccttact gcagcctcga ttgcttcggg caacagtgcg caacaggcgg
     2041 ccagcgtcgg cgccacgctg actgcgatca cctacctcgt tgcggcggga gggaacgccc
     2101 ttggcggagc actgtcggat cgtcatggcc gtactttggt cgcactgttc atgggtataa
     2161 gcagtcttgt tttctcattc agcttcggct ggatgctcgg gctgccaatg tggctactgc
     2221 tgctgatcgc aacgctttac aacgctacca gtgtggccga ttcctcgata ctctcgacga
     2281 cccttacgga acttgtcgac ccagccagcc ttggcgcagc gtattccttg cgctcagttc
     2341 tcgggtttag tgcgggggct gttgcaccct gggttttcgg tgttgtgctc gatgcggggc
     2401 gcgctgccgg cggggtttcg gatacgatcg tatgggccct ggcctggtcg acgctcggac
     2461 tgggaggatt gctcggcccg ctggcgattt ggcggttgcg acaaatgccc gatgcccgtc
     2521 gccttgccgg agggaagaga taagcttcgt taccaaccca ttcacaggag attccatgaa
     2581 aaccagactt ctcgccattt ccggcagtag ccgccaggat tcggtcaaca tgcgcctgct
     2641 caacaccgcg acggacatcg cccgcagaca aggtgccgaa gtgaatgtgc tcgatctgcg
     2701 cacactggcg ctgccgctat atgatggtga tctcgaagcc gcctccggcc tgcccgaagg
     2761 gtgcctccac ctgaaacagg cgattcgcga gcatcacgca ctgcttgttg cctcgccgga
     2821 atacaacggt ttctttacac cgctcctcaa aaattcgatc gactgggcaa cgcgcccaca
     2881 ggaaggcgtg ccttctccat tccccggcaa ggttgccgcg atcatcgccg cttcacccgg
     2941 cgcactgggc ggaattcgcg gattgcccgc cttgcgtatc ctgctcagcg gcatcggcgt
     3001 tctcgtgacg ccagggcaga tgtcattgcc gctggcgcac aaagcctttg atgagaaggg
     3061 cgatctgatc aatccgcagc agcagaaggt gcttgagggc gttgtcggcg aactcctcag
     3121 aacggtccac acagactgag cgcgtgttcg tcgtcggcgt gcatcctgca ggaacgaaaa
     3181 gaaacggggc ggtcgagccg ccccgtttgc cacatcaaat atctactgaa acacgctgtc
     3241 agatgttctc tggctccatg gtaatggcgc cgcaaggaca ctcggccacg cacatgccac
     3301 aacctttgca atagtcaaga ttgaacttga agcgattgcc ggggccgagc ttgatgactg
     3361 cattatccgg acagacgccg taacagttgt cgcactcgaa acagttgccg caggaaagac
     3421 agcgacgcgc ctcgaacaat gccgtgctct catccagacc gccctgcact tcttcgaagg
     3481 tcgatcgacg acggatgatg tcgagcatcg gccgcacggt cttcggcgca tccgcgtagt
     3541 accaggtatt gaggctttcg aagttggcca ccgcgtgctt gggaggagcg atgtagctgg
     3601 ttccgcgaag ccaggcgtcg atgttgcgcg ccgccttctt gccatgcccg acggcaatgg
     3661 tgatgttgcg atcggcagga accatgtcgc caccggcgaa gatgcctgca tggccagtca
     3721 tcatgttggg atcgacggtg acgacaccgt cggccacgac cagacccggc acaccgtcaa
     3781 gcaggccaag atccacatcc tgaccgagtg ccaacacaac cgaatcggcc tccagcgttt
     3841 caaactctcc ggtcggttgc ggaaagccgg tgtcgtcgag ttccattttt tcgatggtga
     3901 tgttcgcgtc gcctgcctcc ttgatggtcg acagccactt gatcatcacg ccttcctgca
     3961 tcgcttcctc gatctcgaaa tcatgcgccg gtgccttttc acggttacgg cgataaacga
     4021 tgatcggctc ggcacccatg cgtttggccg ttctcgccac gtcgattgcc gtattgccgc
     4081 caccatagac gatgacacga cgaccgagca ttggcttttc ttcgttttcc atgctgcgca
     4141 acacagaaat cgcgtcgaca atcttcgaga cggcacccgc cgggataaag gcgcgtttgc
     4201 caatatgtgc cccgacagcc aggaaggccg cgtcgaactt gcccgattcc atcgtttcaa
     4261 gaaggttatc tactttggag ttcagcttca cctcgacgcc cagatcgacg acacgctgca
     4321 tctcggcatc gagaacatcg cgtggcagac ggtacttggg gataccgaag cgcatcatcc
     4381 cgcccatgaa tggtccggca tcgtaaacgg taacgcggtg gccgaagcga cgcagatgat
     4441 aggccgccga catcccagaa ggccccgctc cgacgatcag aacatgcttt ccggtttcgg
     4501 cggcagtctg tgacagcttc cacttgcgct tgatcgcctc gtcgccgaga aagcgctcca
     4561 ccgagttaat gccgaccgcc gagtcgatct tcgcgcggtt acagacgctc tcgcaactgt
     4621 ggtagcaaac gcggcccata atcgccggaa aaggattgtc cttcgtcaga tgctgccagg
     4681 ctttttcgta gttgccggat tcggcgtgga acaaccagcc ctgtatgtct tcaccggccg
     4741 gacattggtt gttgcagggc ggtagccggt cgacatagac cggacgttcg gtacgccacg
     4801 aacccgtgtg gttggcaagc gacgagccgg ggtccagtgt aattgcaaac ggcttgtcca
     4861 tcagtgggcc tccttgtcga gcaattcgta tctgcgaata ttcttgtctg ccattgcctg
     4921 caaacgggca atggtgtcaa cggcaggcgt tttgccaaaa aggtgggcaa aacgcttctg
     4981 tggtttcagg tattcctcga cgggcacccg ccgacgaatc ttcgatgaac cggtgagttc
     5041 gccgtactcg gcttcgaaca ccggaaacgt cccgctctct ttggccagac gggacaagcg
     5101 gatggtgtcc tgagacgctg caccccaacc gagcggacaa ggcacgagaa tctgaatata
     5161 gcgcgcaccg cggaagctca tggctttctt gactttgtat tcgagatcac gcaagtcagc
     5221 aactgtggcc gtcgcgacgt agggaatctc gtgcgccatg gcgatggcag gtacaaactt
     5281 gccctgaccg aagacgtttc caggctccgg gccgaccggc atggtcgttg ccgtacgcgc
     5341 ggagggcgga gtggccgaac tgcgctgaac accggtgttc atgtaaccgc cattgtcgta
     5401 gcagatatac agcacatcgt cgttgcgctc gaacatgcca gagagacaac cgaaaccgat
     5461 atcggtagtt cccccgtcac cgccttgcgc aacaacgcgg acatcgccgc tccggccctt
     5521 ggcctgtttc accttgatcg ccgcagcgat accggtggct accgcagccg tatttccaaa
     5581 tagcgagtga atccatggaa tctgccatga agtttccggg tagggcgtag agaacacttc
     5641 gaggcaaccg gtggcgttgg ccgcaatcaa ctggttgttg ctggcgatca tcgccgagtc
     5701 gatagcgtaa cgcgctccga gagcctcgcc gcagccctgg caggcgcgat gcccggagtt
     5761 aagcgagttg gtgcgctcca tgttggcttg aacgctacgc tcttcaggag ccagcagacg
     5821 attgccgacg gtgaaggtgc cggtctggta aaacttgaca ggttgcaatg acatggttgg
     5881 cctcctttag gcgatcttcg aggcgacagt accaatgtcg cgcagcat
//
LOCUS       DAORPM010000150         5896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3693_length_5896_cov_7.364664, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000150 DAORPM010000000
VERSION     DAORPM010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3693_length_5896_cov_7.364664"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLD53_08080"
     CDS             <1..260
                     /locus_tag="PLD53_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC60910.1"
                     /translation="IALSGGEQQRLAIARAVVNRPAILLADEPTANLDAESAADIFEI
                     FRAFHQVGVTIAIATHDPQWIAHFSPRVLKLDRGRLLGAAS"
     gene            257..1156
                     /gene="ftsX"
                     /locus_tag="PLD53_08085"
     CDS             257..1156
                     /gene="ftsX"
                     /locus_tag="PLD53_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRC60911.1"
                     /translation="MSIFFAQHFTALKDALRRLATSPLNTMLSLLVIGIALALPCAGW
                     VVLDNLHDIAGSASGAQQISIFMSLDASRKDVDEIESRLRDAKPGNWRFVSRDDALKH
                     LQASEGMSEIIANLPKNPLPDAFVVEPVDAQPETLELMAKSFSGWPKVAHVQLDSAWI
                     KRFDAFLRIGKLSVTLLASLFASALVAVTFNTIRLQILGQAAVIEVAKLIGATDAFIR
                     RPFHYFGALQGALGGVLAALLVTLGGELLSAPVGELVALYGGSFVLHGLSPGNIAALA
                     AIGAALGWLGAQMSVSIHLHRIG"
     gene            1192..1851
                     /locus_tag="PLD53_08090"
     CDS             1192..1851
                     /locus_tag="PLD53_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRC60912.1"
                     /translation="MTDADNDRQHQLLGLFDSPFVRRVAVTMNHYGIPFKHVSLSVFR
                     HMDAMRPLNPLFKVPMLTLPNGETLYESAYQLDYLEELAADRGLSPLIPRSGPERRQV
                     QQVMALAIIAAEKSVAMQYERNRPLELQSPDWSRRLRSQMRQAFSMLEERMEGSERTT
                     GNFLFNDRLTQADITTVAGIGFVRYIQPDEWPAGEYPALEALAARLEASEAFRKVPLD
                     E"
     gene            complement(1878..3302)
                     /locus_tag="PLD53_08095"
     CDS             complement(1878..3302)
                     /locus_tag="PLD53_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRC60913.1"
                     /translation="MDDEIELLRYERLAEDLSGIIAAGNLRPGERLPSVRRLSRERRL
                     SVSTVLQALRQLEDRGLVEARPQSGYFVRHASARRAQPSVRSTPEEAVPVDVSQRLMR
                     VLQTGVKPGVAPLSAALPSPALLPLAALQRLYGGVARRHPKLLEGSSHINMDEPALVR
                     QLVLRAVGWAGPQAASEFLITNSCTEALGLCLRATTRPGDTVAVESPAYYLMLQLLEV
                     LGLKALEIPTDPRTGLSIEALDLATRTGRIAACLLVPNASNPLGSIMPDEHKKRLAKL
                     TAERGVAVIEDDIYGELYFGQTPPRPIKAFDQAGNVMLCSSFSKCLSPALRIGFVAAG
                     RYRQQIALHKTVSSGSTNPITQLVLAEYLESGSYDRHMRGLRRAYEHQVDAMRAAVAR
                     HFPPATRITQPLGGFVLWVELPDDIDTTTLYDRAIAAGVAYVPGELFSASGMYRNCLR
                     LNCGNPHTPEIEDAVRRLGELMAS"
     gene            complement(3606..5093)
                     /locus_tag="PLD53_08100"
     CDS             complement(3606..5093)
                     /locus_tag="PLD53_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRC60914.1"
                     /translation="MISRPVERGVGRAMGTDGLVRRMTASWPLLVVSLLMLGGCATRP
                     INPPIAEVHPERGYRLESRQAHVKDKSNLVVLAFSGGGTRAAAFSYGVLEFLRRTRIV
                     GATGKEARLLNQVDVISGVSGGSFTALAYGLYGDKLFSEYESRFLKRDVQGEITARFF
                     SPRYWPNLWSSNWGRSELAADLYDEILFNGATFGDLDRSNGPLIMASATDISTGARLV
                     FDQDFFDLLCSDLDEVPLSRAAAASSAVPVVLSAVTLNNYGGSCNYAAPRWLQLLTGP
                     TGPPRPAARAAREIDERLAFADGAHRPYIHLVDGGIADNVGMRSVLTVLELLEVLHRA
                     GVPTRLDGVRRIIVFVVNSVSSPRTQWDESESPPGILDTLVKAAGVPIDHYSYEAMEV
                     LKDKAARWQSLRRLRQSAAFANNTDPVITDELKAPDIDVYAVDVSFQALDDKAEMDYL
                     NELPTSFVLPAEAVDRLRAAAGSIILQSPEFQRLLKDAGAQVVSQ"
     gene            complement(5083..>5896)
                     /locus_tag="PLD53_08105"
     CDS             complement(5083..>5896)
                     /locus_tag="PLD53_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRC60915.1"
                     /translation="DAVEVRDFVTKEWSLIALAGPANRQGRIAADVIAGRDSRFRGTQ
                     GTSIIGLFSASAAWVGASEKTLKRLGDSDYEKIYLYPNSHAGYYPGARPIAMKILFRK
                     SDGRFLGAQAVGEDGVDKRISALAMALQMGATIYDLEEAELCYAPQFGSAKDPVNFAG
                     MVAADILRGDMPLSHWDATDDAFLLDVREPAELAVESVPGAHNIPMGQLRARLAELPK
                     DREILIVCRSGQRAYYATRVLLQNGFKARTVAGGMLSRVILSMTGESACSDL"
BASE COUNT         1162 a         1911 c         1741 g         1082 t
ORIGIN      
        1 cgattgccct ttccggcggg gaacagcaac ggctcgccat cgcccgggcc gttgtcaatc
       61 ggcctgccat tttgctggca gatgagccaa cagccaatct ggacgcggaa tcggcagcag
      121 acattttcga gatatttcgc gccttccatc aggtgggcgt cacgatcgcc atcgccacac
      181 acgatccgca gtggatcgcc cacttctcgc cgcgagtgct caagctcgac cggggacgac
      241 tgctcggagc tgcgtcgtga gcatcttctt cgcccagcac ttcacggccc tgaaggatgc
      301 gctgcgccga ctcgccacct cgcccctcaa taccatgctg tcgcttctgg tgatcggtat
      361 cgcgctggcg cttccgtgcg ccggctgggt cgttctcgac aatctgcacg atatcgccgg
      421 cagcgcatcc ggcgcccagc agatcagcat tttcatgtcc cttgatgcca gccggaagga
      481 cgtcgacgag attgagtcgc gactgcgcga cgccaagccg ggcaactggc gtttcgtctc
      541 ccgggacgac gctttgaagc atctgcaagc gtccgagggc atgtccgaga tcatcgccaa
      601 cctcccgaag aacccgctgc cggacgcctt tgtcgtcgag cccgtcgatg cgcaaccgga
      661 aacactggag ctgatggcca agtcgttttc cggctggccg aaggtcgcac atgtccagtt
      721 ggattccgca tggatcaaac gttttgacgc tttcctgcgc atcggaaaac tatccgtcac
      781 cctgctcgcc agcctgttcg ccagtgcgct ggttgccgtg acttttaaca ccatccgcct
      841 gcagattttg gggcaagcgg cagtaatcga agtagccaaa ctgatcggcg ccaccgacgc
      901 cttcatccgt cggccgttcc actatttcgg cgctctgcaa ggcgcgcttg gcggtgttct
      961 tgccgcactg ctcgtcacac tgggtggaga gctgctcagc gctccggtag gcgaactcgt
     1021 cgcgctgtac ggtggcagct ttgttctgca cggactgtcg cccggaaaca tcgcggcgtt
     1081 ggccgcaata ggtgccgcac tgggctggct gggcgcacaa atgtcggtca gcattcacct
     1141 gcaccgcatc ggctgaaccc gggtgcacga ccctgacaat cggagatgag tatgacggac
     1201 gcggacaacg ataggcagca ccagcttctc ggccttttcg attcaccctt cgtgcgtcgc
     1261 gtcgccgtga ccatgaatca ctacggcatc cccttcaagc acgtctcgct gtcggttttt
     1321 cggcacatgg acgcgatgcg gccgctcaat ccgctgttca aggttcccat gctgaccctg
     1381 cccaatggcg aaacgcttta cgagagcgcg tatcaactcg attaccttga agaactggcg
     1441 gctgatcgcg gcctctcacc cttgataccg cgctccggcc cggagcggcg gcaggtgcaa
     1501 caggtgatgg cgctggccat catcgccgcc gaaaaatcgg tggcgatgca atacgaacgc
     1561 aatcggccgc tcgaactgca gtccccggac tggagcaggc gcctgcgcag tcagatgcgc
     1621 caggccttca gcatgctcga agaacgcatg gagggaagcg aacgaacgac cggcaacttc
     1681 ctgttcaacg accgcctgac gcaggcggac atcaccacgg tcgccggtat cggctttgtg
     1741 cgctacatcc agccggatga gtggccagcg ggcgaatatc ctgcgctcga agccttggcg
     1801 gcacgtctcg aagccagtga agcgttcagg aaggtgccac tggacgagta acagggttta
     1861 tacgaactac ctcgacttca ggacgccatc agttcgccga ggcggcgcac ggcatcctcg
     1921 atctccggcg tatgcggatt gccgcagttg agtcggaggc agttccgata catgccgctc
     1981 gccgaaaaga gttcgcccgg cacgtacgcg acgcccgcgg caatcgcgcg gtcatagagc
     2041 gtggtggtat cgatgtcgtc gggcagctcg acccacagga cgaagcctcc caacggctga
     2101 gtgatccgcg tcgcaggcgg gaaatgccgg gccaccgccg cacgcatggc atcgacctgg
     2161 tgctcgtagg cccgccgcaa tccgcgcatg tggcggtcgt aagatcccga ctcgaggtat
     2221 tcggcgagaa ccagttgcgt gatcggattg gtgcttccac tgctgaccgt cttgtgcaaa
     2281 gcgatctgct gccggtagcg gcccgcggca acgaaaccga tacgcagcgc aggcgaaaga
     2341 cacttggaaa aagacgaaca gagcatcacg ttgcccgcct gatcgaacgc cttgatcggt
     2401 ctcggcgggg tctgcccgaa gtaaagctcg ccgtagatgt cgtcctcgat caccgccaca
     2461 ccgcgctctg cggtcagctt cgcgagccgc ttcttgtgct catcgggcat gatgctgccc
     2521 agcggattgc tggcattggg taccagcagg caggccgcga tccggcccgt gcgcgtggcc
     2581 agatcgagcg cctcgatcga cagcccggtg cgtggatcgg tcggaatttc cagtgccttt
     2641 agccccagca cctcaagcag ttgcaacatc aggtaatacg ccggcgattc gactgccacc
     2701 gtgtcaccgg ggcgggtcgt tgcgcgcaga cagagcccga gtgcttcggt gcaggagttg
     2761 gtgatcagaa actcgctcgc cgcctgcggg cctgcccagc cgacagcgcg cagtaccaac
     2821 tggcgaacca gcgccggctc gtccatgttt atgtggctgc taccctcgag caacttcgga
     2881 tgacgccggg caacaccacc gtacaggcgc tgaagagcag caagcggcaa cagcgctggc
     2941 gaaggcaatg cggcgctgag gggggcaacc ccgggcttga cgccggtctg cagcacgcgc
     3001 atcaggcgct ggctgacatc gaccggtact gcttcctccg gggtcgagcg gacgctcggc
     3061 tgcgctcttc gtgccgacgc gtgacgaacg aaatacccgg actgcggccg tgcttcgaca
     3121 agccctctgt cttccagctg acgcaatgcc tgcaaaaccg tcgacacgga caaccgccgc
     3181 tcgcgcgaca gacgccgcac cgaaggcaga cgctcgcccg gccgcaaatt gccggcagcg
     3241 ataatcccgc tcagatcctc cgccagccgt tcgtaacgca agagttcaat ttcatcatcc
     3301 atcgaattca ccgaattcac cgaaagggtt gctgccgaaa acgaccggat tacccgccgg
     3361 tgcttcgaca aatacgcatc ctgacgcgaa ataaaccacc acatcaacgc ccgttccccc
     3421 gacagcaagc cgaaggatgt agccgcaccc ctgagcgcgc tccccagcca ctgacgcccc
     3481 catcgtccga cattcagcga agtaccggaa cgccgtagat tatgaatgca gtatatgggt
     3541 gccgatgaat cgtggtcaat ctcgcgggcc agcggctcgc cgccggctac atgaacgatg
     3601 aacgactact gggaaacgac ctgtgcgccg gcatccttca acagccgctg gaattccggt
     3661 gactgcaaga tgatgctgcc tgcggcggca cgcagacgat ccaccgcctc agcgggcagc
     3721 acgaaggacg ttggcaattc gttcaggtaa tccatttcag ccttgtcgtc cagggcctga
     3781 aatgaaacgt ccacggcata aacatcgata tccggggctt tcagctcatc ggtgatcacc
     3841 ggatccgtat tgttcgcgaa cgccgccgat tgccgaagcc gtcgcaggga ctgccagcgt
     3901 gccgccttgt ctttcaacac ttccatcgct tcgtaagaat agtggtctat cggaacacca
     3961 gccgctttga ccagggtatc caggatacca ggtgggctct ccgattcatc ccactgagtc
     4021 cgtggcgagg acaccgagtt cacgacaaag acgatgatcc tacgcacgcc gtcaagccgc
     4081 gttggcacgc ctgcgcgatg caatacctcc agtaattcca gtacggtaag cacactgcgc
     4141 ataccaacgt tgtctgcaat gccaccgtcc acaagatgaa tgtacgggcg atgagcgcca
     4201 tcggcaaacg ccagccgttc atcgatctcg cgcgccgcgc gtgcggccgg ccgcggtgga
     4261 ccggtgggtc cggtcaacag ttgtagccag cgaggtgccg cgtagttgca gctgccgcca
     4321 tagttgttga gcgtaacggc cgaaagcact accggaaccg ccgatgatgc ggcagcggcg
     4381 cgcgacaacg gcacctcatc aagatcggag cacaacagat cgaagaagtc ctggtcgaag
     4441 accagacgag ccccagtcga gatgtcggtt gccgacgcca taatcagcgg gccattgctc
     4501 cggtcgagat cgccgaaggt cgcaccattg aacagtattt cgtcatacag gtcggccgcc
     4561 agctccgagc gcccccagtt gctcgaccac agattcggcc agtaacgcgg gctgaagaat
     4621 cgggccgtga tctcgccctg cacatcgcgc ttcaggaagc gactctcgta ctcggagaac
     4681 aacttgtcgc cgtagagtcc atatgccagc gccgtaaagc tgcctccgga aacgccgcta
     4741 atcacgtcga cttggttcag aaggcgggct tctttacctg tggctccgac gatccttgtg
     4801 cgtcgcagga actccagcac gccatatgaa aaagccgcgg cacgggtacc cccgccggaa
     4861 aaggcgagaa caaccagatt ggatttgtcc ttgacgtgcg cctgccgact ttccaggcgg
     4921 tagccccttt ccgggtgcac ttccgcgatc ggcggattga tcgggcgcgt cgcgcaaccg
     4981 cccagcatga gcagtgaaac gaccagtaac ggccatgaag ccgtcatccg cctcacaagc
     5041 ccatccgtcc ccatggcccg cccgacgcca cgttctaccg gcctagagat cactgcaagc
     5101 gctttctccc gtcatcgaga ggatgacccg tgacagcatt cctcccgcaa cagttcgggc
     5161 cttgaacccg ttttgaagaa ggactcgcgt cgcgtagtag gcccgctggc cggagcggca
     5221 aacgatgagg atttcccgat ccttgggcag ttcggcgaga cgtgcccgca gttgccccat
     5281 cgggatgttg tgcgcacccg gaacgctttc gaccgcgagt tccgcgggct cgcgcacgtc
     5341 gaggagaaat gcgtcgtcgg tggcatccca gtgactcagc ggcatgtcgc cgcggaggat
     5401 atcggcggca accatcccgg cgaaattcac cggatccttg gcgctgccga attgcggcgc
     5461 gtagcacagc tcggcttcct cgaggtcgta gatcgtcgca cccatttgca gcgccatggc
     5521 cagcgcgctt atccgcttgt ccacgccatc ctcgccgacc gcctgcgcgc cgaggaaacg
     5581 cccgtcggat ttccggaata gtatcttcat cgcgatcggc cgcgcacccg ggtagtagcc
     5641 cgcgtgcgaa ttcgggtaga ggtagatctt ctcgtagtcg ctgtcaccga gccgcttgag
     5701 cgtcttttcg ctggcgccga cccaggcggc ggatgcactg aaaagtccga tgatcgaggt
     5761 gccctgggtg ccgcggaagc gggagtcgcg tccggcaatc acatcggcag cgatgcggcc
     5821 ctgtcggttt gccggtccgg caagcgcgat gagactccac tccttggtca cgaaatccct
     5881 gacttcgacg gcgtcg
//
LOCUS       DAORPM010000151         5860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3732_length_5860_cov_11.595521, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000151 DAORPM010000000
VERSION     DAORPM010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3732_length_5860_cov_11.595521"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PLD53_08110"
     CDS             complement(<1..933)
                     /locus_tag="PLD53_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60916.1"
                     /translation="MSFLPVLLWSDVLIWLLVLAAIVLGVLSSRNPPLRAAWQRVGRS
                     RQGMAAATVLMVFILVGLLDSVHYRPRLESREADQPDVYAIEVLSLLDALVTPLRTRN
                     EKTYSEPLATHAYAKESIEVRDASGGVGQVRDFPRLKHGGVHLGDRDDQRNADVGQRV
                     AAAGVLACALWGVMALGCVHLLARRNARPARRMWSMIWRGETDFAWNAVLATLGLLLV
                     LLLPLVALCVDYHVFGTDKVGQDVLYQVLKSIRTALVIGLVTTLVMLPLSILLGILAG
                     YFRGWVDDGIQYLYTTLSSIPGVLLIAAAVLMMQV"
     gene            complement(1039..2016)
                     /locus_tag="PLD53_08115"
     CDS             complement(1039..2016)
                     /locus_tag="PLD53_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC60917.1"
                     /translation="MLAYLLRRLLYAIPILIGVNLITFALFFVVNTPDDMARMQLGVK
                     RVTPEAIAKWKVERGYDKPLFINSQAKGIGVLTDTVLYTKSARMFVGDFGRAEDGRDI
                     AREITTRMGPSLAIALPTFILGLFVTVSFALLLAFFRASYLDFWGVVLCVALMSISSL
                     FYIIGGQFLISKLWRLVPISGFSGGLEAWKFVMLPVAIGVISGIGSSARWYRTIFLEE
                     IGKDYVRTARAKGLSELIVFIRHVLRNAMIPILTGVVVVIPLLFMGSLLTESFFGIPG
                     LGSYTIDAINAQDFAVVRAMVFIGSVLYIVGLMLTDISYTFVDPRIRFE"
     gene            complement(2325..2696)
                     /locus_tag="PLD53_08120"
     CDS             complement(2325..2696)
                     /locus_tag="PLD53_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRC60918.1"
                     /translation="MILVDTSIWIDHLRSGSPALGTLLENEAVCTHDFVIGELACGNL
                     RNRTEVLGLLQSLPRLSAATEDEALFFIEQQQLMGCGIGYIDVHLLAAAVIRNFPIWT
                     KDKRLMAVAEEKGWAYVPGAH"
     gene            complement(2693..2890)
                     /locus_tag="PLD53_08125"
     CDS             complement(2693..2890)
                     /locus_tag="PLD53_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRC60919.1"
                     /translation="MRTTVTLDETLLNRAQLLSGVQERSALLKEALNALIQRESARRL
                     ARLGGTDPQLESVARRREGKR"
     gene            complement(3132..5312)
                     /locus_tag="PLD53_08130"
     CDS             complement(3132..5312)
                     /locus_tag="PLD53_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC60920.1"
                     /translation="MLVFRLACLSVFLLLAACSSEQNDPYPLSERGKNIYYAAFTERP
                     KHLDPVQSYTEDEITFTAQIYEPLLQYHYLKRPYTLIPATAEAVPVPRYYDAGGKALP
                     ADAPASDIAHSIYEIRIRPGIHYQPHPAFALDQDGKPVYADLDPAKLADIETIADFTR
                     TGTRELTADDYIYEIKRLAHPRLHSPIFGMMAERIVGLKELGEVLHKEAKSLPAGTWL
                     DLDRFALKGVTRVNRHTLRIELRGKYPQFLYWLAMPFFAPVPREVDRFFGQPGMAEKN
                     LTLDWYPVGTGPYMLTENNPNRRMVLERNPNFHGETYPCEGEPGDRAAGLLADCGKPL
                     PLIDKAVFSREKESIPYWNKFLQGYYDASGISSDSFDQAVRFNVGGDVQLSDEMRAKG
                     IRLLTSVRLSTFYMGFNMLDAVVGGKDGIGSERSAKLRQALSIAIDQEEFISIFMNGR
                     GIAAMSPLPPGIFGYLDGEAGMNPVVYDWVDGKARRKPVAVAKRLLAEAGWPDGRDSR
                     TGAPLVLNLDTTSGGMGDKSRLDWLTRQFAKLDVQLVVRSTDFNRFQEKLRKGAEQLY
                     YLGWNADYPDPENFFFLLAGSESKVEKGGENTSNYASPEFDRLFAEMKDMESDGPSGP
                     RRLSIITRMNRILQHDAPWIFGFHPKSYTLGHSWLYNRKPMDIGNNILKYQRIDAAER
                     EKLRRQWNEPVLWPLALIVVVLAAVILPAVISYRRRERGTARGP"
     gene            5501..>5860
                     /locus_tag="PLD53_08135"
     CDS             5501..>5860
                     /locus_tag="PLD53_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRC60921.1"
                     /translation="MGFLAGKRILITGLLSKHSLAYGIAKACHREGAQLAFTYQNERF
                     LDRINKFSAEFGSTLVFQCDVSDDAQIDKVFADLATHWEGLDGLLHSIAYAPTEAIEG
                     DFLDGITREAFRIAHDVS"
BASE COUNT         1293 a         1725 c         1691 g         1151 t
ORIGIN      
        1 cacctgcatc atcagcacgg cggcggcgat cagcagcacg ccggggatcg aactgagcgt
       61 cgtgtaaagg tactggatgc cgtcatcgac ccagccacga aagtagccgg cgagaatgcc
      121 cagaagaatc gacaggggca gcatgaccag tgtggtgacc agcccgatga ccagcgccgt
      181 gcgtatgctc ttgaggacct ggtagagcac gtcctgaccg accttgtcgg taccgaaaac
      241 gtggtaatca acgcagagcg cgaccagcgg caacagcagc acgagcagca ggcccagcgt
      301 ggccagcacg gcgttccacg cgaaatccgt ttcgccgcgc cagatcatgc tccacatcct
      361 gcgcgcaggg cgtgcattcc tgcgcgccag caaatgcacg catcccagag ccatcactcc
      421 ccacaacgca caggcaagca caccagccgc cgccacccgt tggccaacat cggcgttgcg
      481 ctggtcgtcg cgatccccca gatggacgcc accgtgcttc aggcgcggga aatcgcgaac
      541 ctggccaacg ccgccgctcg catcgcgcac ttcaatggat tccttggcgt aggcgtgcgt
      601 ggccagcggt tcggaatagg ttttctcgtt gcgcgtgcgc agcggtgtta ccagcgcatc
      661 gagcagcgaa agcacttcga tggcatagac atccggctgg tccgcctcgc ggctttcgag
      721 ccggggccga taatgaaccg agtcgagcag accgaccagg atgaaaacca tcagcaccgt
      781 cgcggccgcc attccctgcc ggctacgccc gacccgctgc caggcggcgc gcaagggcgg
      841 atttcgcgac gacagaacgc cgagcacaat cgccgccaat accagcagcc agatcagcac
      901 gtcggaccac aacaggacgg ggagaaaact cattacaagc gcaccaccaa gtcaccaaga
      961 tcacgaaggc tcaccaagaa aaccattaag aatgcttggt gcaacttggt gctcttggtg
     1021 acttggtggt gaaaatcgtt attcaaatct gatcctcgga tcgacgaaag tgtaggagat
     1081 gtcggtgagc atcaagccga cgatgtacag caccgagccg atgaaaacca tggcgcgaac
     1141 cacggcgaaa tcctgcgcat tgattgcatc gatggtgtag ctgcccagcc ccggaatacc
     1201 gaagaaggat tcggtgagca gcgaacccat gaacagcagc ggaatgacca cgacgacgcc
     1261 tgtcagaatt ggaatcatgg cattgcgcaa cacatgtcgg ataaacacaa tcagctcgga
     1321 aagccccttg gcgcgcgccg tgcgcacgta atccttgccg atttcttcga ggaagatcgt
     1381 tcgataccag cgcgccgacg aaccgatgcc ggaaatgacg ccgatggcca ccggcagcat
     1441 cacgaatttc caggcttcca ggccgccact gaagcccgag atcggcacca gtcgccataa
     1501 cttgctaatc aggaattgtc cgccgatgat gtagaagagg ctggaaatcg acatcaaggc
     1561 aacgcagaga acgacgcccc agaaatcaag ataactggca cgaaagaaag ccagcagcag
     1621 cgcaaaactg accgtcacga agaggccgag gatgaatgtc ggcaaggcaa tggccagcga
     1681 aggccccatg cgcgtggtga tttccctggc aatgtcgcgg ccgtcctccg cccgcccgaa
     1741 gtcgcccaca aacatgcgcg ccgacttggt gtacaggacg gtgtcggtca gcacgccaat
     1801 acctttcgcc tgcgaattga tgaacagcgg cttgtcgtag ccgcgctcga ccttccactt
     1861 ggcaatcgcc tcgggcgtca cgcgcttcac cccgagctgc atccgcgcca tgtcgtcggg
     1921 cgtattgacg acgaaaaaca gcgcaaaggt gatcaggttc accccgatca gaatcggtat
     1981 cgcataaagc agtcggcgca ggaggtaggc aagcatgggt gacttatgag tgaataaaac
     2041 cgataaccac agaggcgcag agacacagag ggctcacaga gaaggcgtcg ttcttatctg
     2101 tggattttct ctgtgtctct gtgtctctgt ggttcatatg ggtctgactc atttcacagt
     2161 cctcgcgatg catgggattg tcgcagttca cactgccgcc attcgacaat tgccggagaa
     2221 atctcgggac tttcaagccg agcgtccacc aatcgaaaga actgctcaca ctatcgaacc
     2281 atagccgacc gtcactattc ggttgatctt tgacgttttc ggcatcagtg tgctcctggg
     2341 acataagccc agcccttttc ttcggcaacg gccatgaggc gcttgtcttt cgtccatatc
     2401 gggaagtttc ggatcacggc agcggcaagc aggtgaacat cgatgtaacc gattccacat
     2461 cccatgagtt gctgctgctc gatgaagaat agagcttcat cttcggtcgc agcggacagt
     2521 cgcgggagag attgaagcag gcccaacact tccgtcctgt tacgcagatt gccgcacgct
     2581 agttcgccga tcacgaagtc gtgggtgcag acggcttcgt tttccagcag cgtaccgagt
     2641 gccgggctgc cgctgcgcaa gtggtcgatc cagatcgatg tatcgaccag tatcaccgct
     2701 ttccttcgcg acgtcgggcg acactttcca actgcggatc ggtaccgccc aaccgcgcca
     2761 ggcgacgggc gctttcccgc tggataagag cgttcaaagc ttctttgagc agggcactgc
     2821 gttcttgaac accagagagc agttgtgccc ggttgagaag ggtttcatcc agggttactg
     2881 tagttcgcat cgtgtggctc ctaacgtatc tgcaccaatt atagtatcac tcgatgccaa
     2941 caaagatgat cctgttggat cggcgtacca gcctgaaacg cgaatggaat gcacccatcg
     3001 agcgaactgt tctggccaag gcggccattc gacacgttga agacagagag agaatccaaa
     3061 aggaaggacg ccttctccgt gagtcctctg tgtctttgtg cctctgtggt tcaaacgctt
     3121 tttgactcaa ctcatggccc ccgcgccgta ccacgttcgc gtcggcgata gctgatcacg
     3181 gccggcagaa ttaccgccgc aagaacaaca acaatcagtg ccagtggcca cagtaccggc
     3241 tcgttccatt ggcggcgaag tttttcacgc tcggccgcgt cgatgcgctg gtacttgagg
     3301 atgttgttgc cgatatccat cggcttgcgg ttgtacaacc aggagtgacc gagcgtatag
     3361 ctctttggat ggaagccgaa gatccacggc gcatcgtgtt gcaggatgcg gttcatgcgc
     3421 gtgatgatgg acaaccggcg cgggccgctc gggccatcgc tttccatgtc cttcatctcg
     3481 gcaaagaggc ggtcgaattc gggactggca taatttgagg tgttctcgcc gcctttttcc
     3541 accttgcttt cggaacccgc cagcaggaag aagaaattct ccgggtcggg atagtccgcg
     3601 ttccagccaa gataatagag ctgctccgcg cccttgcgca gcttctcctg aaaacggttg
     3661 aagtcggtcg agcgcacgac gagctgaacg tcgagcttgg cgaactggcg cgtcagccag
     3721 tcgagccgcg atttgtcgcc cataccaccg ctggtggtgt cgaggttgag caccaacggt
     3781 gcaccggttc gagaatcgcg gccatccggc cagcccgcct cggccagcag tctcttggcc
     3841 acggcaaccg gcttgcgcct tgccttcccg tcgacccagt cgtagaccac cggattcatt
     3901 cccgcttcgc cgtcaagata gccgaaaatc ccgggcggca gcggactcat cgccgcgatt
     3961 ccgcgaccgt tcatgaagat cgaaatgaac tcctcctgat cgatcgcaat cgacagggct
     4021 tgccgtagct ttgcgctgcg ctcgctaccg atgccgtcct tgccgccgac caccgcatcc
     4081 agcatattga agcccatata gaaggtcgag agccgcacgc tggtcagcag gcgaatcccc
     4141 ttggcgcgca tttcgtccga gagctgcacg tcgccaccga cattgaagcg cactgcctga
     4201 tcgaagctgt cggacgaaat acccgaagca tcgtagtagc cttgcaggaa cttgttccag
     4261 tagggaatgc tttccttttc gcgggaaaac accgctttgt cgatcagcgg caggggtttg
     4321 ccgcaatccg ccagcagtcc ggcggcccgg tctccaggct cgccttcgca ggggtaggtc
     4381 tcgccgtgaa aattgggatt gcgttcaagc accattcggc ggttcgggtt gttctcggtc
     4441 agcatatagg gaccggtgcc caccgggtac cagtcgagtg tcaggttctt ctcggccatt
     4501 cccggctggc caaagaagcg atcgacctcg cgcggtaccg gggcaaaaaa aggcatcgcc
     4561 agccagtaga gaaactgcgg atacttgcca cgcagctcga tgcgcaaggt atggcggttg
     4621 acgcgcgtca cacccttgag cgcaaagcgg tcaaggtcaa gccaggttcc ggcgggcaga
     4681 ctcttggcct ccttgtgtag tacttcgccg agttctttca gcccgacgat gcgttccgcc
     4741 atcatgccga aaatcggcga atgcaggcgg ggatgggcca gccgcttgat ctcgtagatg
     4801 tagtcgtcgg cggtcagttc gcgcgttccg gtgcgcgtga aatcggcgat ggtttcaatg
     4861 tccgccaact tggccgggtc aaggtccgca tagaccggct tgccgtcctg atcaagcgcg
     4921 aaagcgggat gcggctgata gtggataccg ggacggatgc ggatctcgta gatactgtgt
     4981 gcgatgtcac tggctggcgc atcggccggt agcgccttgc ccccggcatc gtaatagcga
     5041 ggtaccggaa cggcctccgc cgtcgctgga atcagcgtgt aggggcgctt caggtagtgg
     5101 tattgcagca gcggctcgta aatctgcgcc gtgaatgtga tttcgtcttc ggtgtaggac
     5161 tgcaccggat caagatgctt ggggcgctcg gtgaacgccg catagtagat gttcttgcca
     5221 cgttcgctga gcggatacgg atcgttctgt tcgctgctgc aagcagccag cagcaaaaat
     5281 acagaaaggc aggcaaggcg gaaaacaagc atgatgcgaa gtgtaacccg tgtgcggctt
     5341 tcgcagacgc ggtaaaatcc acaatgattg taggggctga ttgccggtca gaaaacggtc
     5401 attggcggtt gggcaatatc acctgcgaaa tcacctataa tgccggctca ggctggcttt
     5461 ggctgcctgg gtacaacaac attgagtcat gggagaacac atgggattcc tcgctggaaa
     5521 gcgcattctc atcaccggcc tgctgtcgaa gcattcactt gcctacggga tcgccaaagc
     5581 ctgccatcgc gaaggcgcgc aattggcctt tacctatcag aacgaacgtt tcttggaccg
     5641 aatcaacaag ttttcggcag agttcggcag caccctggtt ttccagtgcg acgtctccga
     5701 cgacgcacag atcgataagg tcttcgccga cctggccaca cactgggaag gactcgacgg
     5761 cctgctgcat tcgattgcct acgccccgac cgaagccatc gagggcgact tccttgacgg
     5821 catcacgcgt gaagcattcc gtatcgctca cgatgtgtca
//
LOCUS       DAORPM010000152         5851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3747_length_5851_cov_13.062284, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000152 DAORPM010000000
VERSION     DAORPM010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3747_length_5851_cov_13.062284"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLD53_08140"
     CDS             <1..1335
                     /locus_tag="PLD53_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRC60922.1"
                     /translation="LTNFRVTPDPGVIEVNIQPSASWDQLVERTTHLYEMAHLSRLTS
                     EKFMLDGRHTGTGGGNHFVFGALTALDSPFLRRPDLLRSLISYWHNHPSLSYLFSGLF
                     IGPTSQAPRVDEARNDSVYELEIAFSQFPHPEETVAPWLIDRLLRNLLIDATGNTHRA
                     EFCIDKLYSPDGPTGRLGLLEMRAFEMPPHARMSLAQQLLLRALIARFWKQPYAPGRL
                     LRWGTELHDRFMLPHFVKQDFDDVIGELRDAGYAMESEWFAPHFEFRFPLHGSFVAEG
                     IRFELRHALEPWHVMGEEGSAGGNVRYVDASCERVQVKVDGMSGDRYVLTCNGKPVPL
                     QPTGNVGEFVAGVRYRAWLPESSLHPTIGVHAPLVFDLVDTWMKRSLGGCQYHVEHPG
                     GRNPERYPVNANEAEGRRLARFMAIGHTPGRIDIPLAPRNRSFPFTLDLRQS"
     gene            1373..3949
                     /locus_tag="PLD53_08145"
     CDS             1373..3949
                     /locus_tag="PLD53_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRC60923.1"
                     /translation="MPHSLLSIYPKNTDRFDEMLAEGGALRSSWQTFFDHLVAATPEQ
                     MRHRLDYVRRRIQENGVTYNVYADPKGTDRPWELDPLPLILSASEWHEVSSAVTQRAR
                     LLNAVLGDLYGEQTLLRDGTLPSALVYGHNGFLWPCHGVKPPGDTWLHLYAADLARSP
                     DGRWWVIRDRTQSPSGAGYALENRIIVSRVFPEQFRDMGVQHLASFFRSVRDSLSHWA
                     PHNGKSEREGGDAPLIVLLTPGPYNETYFEHSYLARYLGFPLVEGQDLTVRGECVYLK
                     TLTGLCRVHAILRRLDDDFCDPLELRGDSALGIPGLLQVVRAGKVLVANALGSGLLES
                     AALPGFLPGACEKLLGEPLAMPSVGTWWCGESAALEFTIQNIDRLVIKGTFPSQRMDP
                     IFGNQLDGQQREDLIARLRARPLAYVAQELVNFSQAPVWDSTDDRHLLPRGVALRVYV
                     AATPQGYMVMPGGLTRVSAAANARMISMQRGGSSKDTWVLTDEEVNAFTLLKNAIGVA
                     DLVRSGSNLSSRVVENLFWFGRYSERCEDVARILRVGLSRLVDTGTEAMPALLSTYDL
                     CRSLKLLPGSDAEADGKGKRERADQEEVHRPELESQMLAAISDDEWQDGLAGNIRRLL
                     WVGSQVREHFSLDNWHALNRLQHHLQVYSEFGREIGDALAFLDQILLSSSSLAGFAMD
                     NMTRDDGWRFLFIGRRIERLIFLSSSISQFLRLESTRARGGIEWLLELTDSIITYRSR
                     YMTQPELLPALDLIVFNEDNPHAVYFQLLDLKQYLNRLSTEHGDPDEDLLNQAMSRLR
                     AFDLSSFEGQDFSQCRNCNPCFELAGLLDEIANAAASLSDRMALRYFAHIGEIGRQTL
                     TA"
     gene            3957..4865
                     /locus_tag="PLD53_08150"
     CDS             3957..4865
                     /locus_tag="PLD53_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRC60924.1"
                     /translation="MNSLPARYHVIHETGYNYDSPVSLSRQQLHLTPRECPWQQSITH
                     RLLIEPEPTFSHARFDHFGNPVSMFAIEAPHSSLVVRAESVIEVQPHLPADGLSASPP
                     WESVRNALVYGAAPVLLEANRYRFESPYVRVKHEFNDFASHCFTPNRPLLEAVGALMS
                     HIYNEFEFDPKATTVSTPVLKLLEERKGVCQDFAHLMLACLRSHGLAARYVSGYLLTK
                     PPPGKPRMVGADASHAWVSVYCPSAGGEFWIDFDPTNNLMPDTEHITLAWGRDFGDVS
                     PLRGVILGGDTQEPEVAVTVTPSSES"
     gene            complement(4905..5759)
                     /locus_tag="PLD53_08155"
     CDS             complement(4905..5759)
                     /locus_tag="PLD53_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC60925.1"
                     /translation="MILLFRLLSRLPLGGLHALGAVLGWLAWLLSPTYRRHLRENMVL
                     ALGEDGMRRVRPAAIAHAGRASLEVARIWLRPLEEATALVVKVSGWELVEAATKTGKG
                     IVYLTPHLGCFEVTAQYLSERAPITVLYRPPKQAWLQTLIESGRARAQLRIAAADLAG
                     VRALLKALRRGETVGMLPDQAPRAGEGRWLNFFGRPAYTMTLAARLTESGATTIMVWA
                     ERLAGGAGYHFHLQQPTQAIQGTTEERAQQISHEIEHLIRQCPEQYLWGYNRYKRPRG
                     VEPPPERI"
BASE COUNT         1078 a         1768 c         1728 g         1277 t
ORIGIN      
        1 ctgaccaact tccgcgttac ccccgacccc ggcgtcatcg aagtcaatat tcagccttct
       61 gccagttggg atcaattggt cgagcgtacg acgcacctct acgagatggc gcatctgtcg
      121 cggttgacca gcgagaagtt catgctcgac ggccgccata ccggcaccgg tggcggcaat
      181 cattttgtat tcggcgcgct gaccgcgctc gattcgcctt tcctgcgccg ccccgacctg
      241 ctgcgcagcc tgatcagtta ctggcataac catccttcgc tgtcctatct cttctccggc
      301 ctgttcatcg gcccgaccag ccaggcgcca cgcgttgacg aggcgcgcaa cgattccgtc
      361 tatgagctgg aaatcgcctt cagccagttt ccgcacccgg aagagaccgt tgctccgtgg
      421 ctgattgacc gcctactgcg caaccttctg attgacgcca ccggtaacac gcaccgcgcc
      481 gaattctgta ttgacaaact ctactcgccg gatggtccaa cgggtcgcct cggactactt
      541 gagatgcgcg cattcgagat gccgccgcac gcgcgcatgt cgctcgctca gcaactgctg
      601 ctgcgtgcgc tgatcgcgcg cttctggaaa cagccgtatg cccctggccg gctgttacgc
      661 tggggaacag agttacacga tcgcttcatg ctgccgcatt tcgtgaaaca ggatttcgac
      721 gatgtgatcg gtgagttgcg tgacgccggt tatgcgatgg agagcgaatg gttcgcgccg
      781 catttcgagt tccgcttccc gctgcacggc agttttgttg ccgagggcat ccgttttgaa
      841 ctgcgccatg cgctggaacc ctggcatgtg atgggcgagg aaggaagtgc cggtggcaac
      901 gtgcgctatg tcgatgcctc atgcgagcgc gtgcaggtca aggtcgacgg catgtcgggc
      961 gaccgctatg tgctgacttg caacggaaag cctgtgcctt tgcagccgac cggaaatgtc
     1021 ggcgagtttg ttgcaggtgt ccgttatcgt gcttggttgc cggaatcctc tctgcatccg
     1081 accatcggcg tccatgcacc gcttgttttc gaccttgtcg acacctggat gaagcgctcg
     1141 ctgggcggtt gtcagtatca tgtcgagcat cccggcgggc gcaatcccga acgttatccg
     1201 gtcaacgcca acgaagccga aggccggcgt ttggcgcgct tcatggcgat cggccatacg
     1261 ccgggacgga ttgatattcc gcttgcgcca cgcaaccgga gcttcccgtt tacgctagac
     1321 ttgcggcaat cctgaaacac gggagccgta tagtcgccgg ctcccttttt tgatgccgca
     1381 tagcctactc tccatatatc cgaagaatac cgaccgcttt gacgagatgc tcgccgaagg
     1441 cggcgctctg cgctcgtcgt ggcaaacgtt ttttgatcac ctggtcgcgg ctacaccgga
     1501 gcagatgcgt caccggcttg attatgtccg gcgacgcatt caggaaaacg gtgtcaccta
     1561 caatgtttac gccgatccca aaggcaccga ccgcccttgg gagctcgatc cactaccgct
     1621 aatcctgtcc gccagcgagt ggcacgaagt atccagcgcc gttacacagc gcgcgcgcct
     1681 gctcaacgca gtgctgggcg atctttacgg cgagcagacc ctgctcaggg atggcaccct
     1741 gccgtccgcg ctggtctatg gtcacaacgg gtttctttgg ccgtgtcatg gcgtcaaacc
     1801 gcccggcgat acctggctgc atctttatgc cgccgatctt gcgcgctcgc cggatgggcg
     1861 ctggtgggtg atacgcgacc gcacacagtc accctcgggc gcgggctatg cactggaaaa
     1921 ccggatcatc gtatcgcgtg tgttcccgga gcagttccgc gacatgggtg tgcaacatct
     1981 ggcctcgttc ttccgatcgg tgcgtgacag cctgtcgcat tgggcgccgc ataacggcaa
     2041 atccgagcgg gagggtggag atgcgccgct aatcgtcctg ctgactcccg gtccctacaa
     2101 cgagacctat ttcgagcact cctatctggc gcgctacctc ggctttcctt tggtcgaagg
     2161 gcaggatctc acggtgcgtg gcgagtgtgt ctatctcaaa acactgaccg gactgtgccg
     2221 ggtgcacgcc attctgcgcc ggctggacga tgatttctgc gatccgctcg aacttcgcgg
     2281 cgactcggcg ctgggtatcc caggcctgtt gcaggtcgta cgtgccggca aggtgctggt
     2341 tgcgaatgcg ctgggtagcg gtctgctcga atcagcggca ctgcctggct tcttacccgg
     2401 tgcatgcgaa aaattgcttg gcgaaccgtt ggccatgccg tcggtcggca catggtggtg
     2461 tggagagtcc gcggctctcg agttcacgat tcagaatatc gaccgactgg tcatcaaggg
     2521 tacgttcccg tcacaacgca tggatccgat cttcggcaat cagctcgacg gtcagcaacg
     2581 cgaagatttg atcgcccgcc tgcgcgcccg gccgctggcc tacgtagcgc aggaactggt
     2641 caatttttcg caggcaccgg tgtgggattc gaccgatgac aggcaccttc tgccgcgcgg
     2701 tgtggcgctg cgggtttatg ttgcggccac gcctcaggga tacatggtca tgccgggggg
     2761 gttgacgcgc gtttccgccg cggccaatgc gcgcatgata tcgatgcagc gtggtggatc
     2821 gagcaaggac acctgggtgc ttaccgacga ggaagttaac gcctttacct tgctgaagaa
     2881 cgcgatcggc gtagccgatt tggtacgaag cgggagcaat ctgtcttcgc gcgtggttga
     2941 aaatcttttc tggttcggcc gctattccga gcgttgtgag gatgtggcac gcattctgcg
     3001 cgtcggactt tctcgtctgg tcgacacagg aaccgaagcg atgccggcac tgctgtcgac
     3061 ttacgacctt tgtcgttcgc tcaagctgtt acccggaagt gatgctgaag ccgacggcaa
     3121 ggggaaacgc gaaagggccg accaagagga ggttcatcgg cctgaacttg aatcgcagat
     3181 gcttgccgcc atcagcgacg atgagtggca agacggactg gccggcaaca tacgccgcct
     3241 gctgtgggtt gggtcgcagg tgcgcgagca tttttcgctc gacaactggc acgcgctcaa
     3301 ccgtttgcag caccatttgc aggtgtatag cgagttcggg cgtgaaatcg gtgacgcact
     3361 ggctttcctt gatcaaatac tgctctcctc ctcgtcgctg gccgggttcg ccatggacaa
     3421 catgacgcgc gacgatggct ggcgtttcct gttcatcggc cgtcgaatcg aacgactgat
     3481 tttcctgtcc agttcgatca gtcaattcct gcgcctggaa tctacgcgag ccaggggggg
     3541 tatcgagtgg ctgctcgaat tgaccgacag catcatcacc taccggtcgc gttacatgac
     3601 tcaacccgaa cttttacccg cgctcgatct gatcgttttc aacgaggaca atccgcacgc
     3661 ggtgtatttc cagttgctgg acctgaagca gtatctcaac cggctgtcca ccgagcacgg
     3721 tgatccggac gaagacctgc ttaaccaggc aatgtcacgc ctgcgggctt tcgatctgag
     3781 cagcttcgag ggacaggact tctcacagtg ccgcaattgc aatccctgtt ttgaactggc
     3841 cggtctgctc gatgaaatag ccaatgcagc cgccagcctt tcggaccgga tggccctgcg
     3901 ctatttcgcg catatcggcg aaatcggccg gcaaacgttg acggcgtgac agcttcatga
     3961 attcactccc cgctcgctat catgttatcc acgaaacggg ttacaactat gacagcccgg
     4021 tttcgctgtc gcgtcagcaa ctgcatctga cgcctcgcga gtgcccgtgg caacagagca
     4081 ttacgcatcg gttgcttatc gaacctgaac cgaccttttc gcatgcgcgc tttgaccact
     4141 tcggcaatcc ggtcagcatg ttcgccatcg aggcaccgca tagcagtctg gtcgtgcgcg
     4201 ccgaaagcgt tatcgaagtg caaccgcatc ttcctgccga cgggttgtca gcgtcgcctc
     4261 cctgggaaag cgtgcgtaac gcgctggtct acggtgccgc accggtcttg ctggaggcca
     4321 atcgctatcg attcgaatcc ccttacgttc gcgtcaagca tgaatttaac gacttcgcca
     4381 gtcactgctt tacaccgaat cgtccgctgc ttgaggcggt cggtgcgctg atgagccata
     4441 tctataatga attcgagttc gatcccaaag ccaccacggt ctctacgccg gtgctcaaat
     4501 tattggaaga gagaaaaggc gtttgccagg attttgccca cttgatgctg gcctgcctcc
     4561 gttcgcacgg gctggctgca cgttacgtca gcggctatct gctcacgaaa ccgccgcctg
     4621 ggaaaccgcg catggttggt gccgacgcct cgcatgcctg ggtatccgtc tattgcccga
     4681 gcgccggtgg agagttctgg atagattttg atccgaccaa taacctgatg cccgacacag
     4741 agcacatcac gctggcatgg ggacgggatt tcggcgatgt gtctccgcta cgcggcgtca
     4801 ttctgggcgg cgatacgcag gagcccgagg tagccgtaac ggtcacccct tccagcgaaa
     4861 gctgagcccg acttttggcg catttcaggc gtacacaggt cgcgtcaaat cctctctggt
     4921 ggcggttcga cgccgcgcgg gcgtttatag cggttgtagc cccacagata ctgctccgga
     4981 cactggcgga tcagatgttc gatctcgtgg ctgatttgtt gggcgcgttc ctcggttgtt
     5041 ccctggatgg cttgcgtcgg ttgttgcaga tgaaaatgat agccggcgcc gcctgccagc
     5101 cgctcagccc agaccatgat tgtcgtcgcg ccgctttcgg tcagccgcgc cgccagcgtc
     5161 atcgtatacg ccggccggcc gaagaagttc agccatcgcc cctcgcctgc ccgcggtgcc
     5221 tgatcgggca gcataccgac cgtctcgccg cgtcgcagcg ctttcagcag agcacgcacg
     5281 ccggccagat cggccgccgc gatacgcaat tgcgcgcgtg cacggccact ctcgataagc
     5341 gtttgcagcc aggcttgctt gggtgggcga tacagcacgg tgatcggcgc gcgctccgac
     5401 aggtactggg cggtcacctc gaaacagccc agatgcggcg tcagatacac aattcccttg
     5461 ccggttttcg ttgccgcttc gaccagttcc cagccgctta ccttgacgac cagggcggtc
     5521 gcttcttcca gcgggcgcag ccagattctg gctacttcaa gcgatgcacg gccagcatgc
     5581 gcgatagctg ctggccgcac acggcgcatt ccgtcttcac cgagcgccag taccatgttt
     5641 tcgcgcaggt ggcggcggta ggttggtgac aggagccagg ccagccagcc gagcacagca
     5701 ccgagtgcat gcaacccccc caagggcaaa cgactaagca gacgaaagag aaggatcacg
     5761 ggttagccct gttggaaggg gattgttggg ttgttcgatt gtttgattgt tgaaagcggg
     5821 tttgacccgc ggcacacgaa aaactacaat t
//
LOCUS       DAORPM010000153         5802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3805_length_5802_cov_14.876805, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000153 DAORPM010000000
VERSION     DAORPM010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_5802_cov_14.876805"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /gene="secA"
                     /locus_tag="PLD53_08160"
     CDS             complement(<1..666)
                     /gene="secA"
                     /locus_tag="PLD53_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRC60926.1"
                     /translation="MIPGLLKKFFGSRNDRLIKQYSAAIRKINALEAGISALSDDDLR
                     GKTAEFKARVANGEELDALLPEAFAVVREAGKRSLGMRHFDVQLVGGMVLHDGKISEM
                     RTGEGKTLVATLPAYLNALAGKGVHIITVNDYLASRDSEWMGRLHRFLGLTVGVNLSQ
                     MAHDDKQLAYAADITYGTNNEFGFDYLRDNMVYTTRERVQRKLSYAIVDEVDSILIDE
                     ARTP"
     gene            complement(779..1807)
                     /locus_tag="PLD53_08165"
     CDS             complement(779..1807)
                     /locus_tag="PLD53_08165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRC60927.1"
                     /translation="MHIILVSNRLATAKTLNLSYRTLLALLSGVIILAFATSFLFSWV
                     GVRFNMPFAAELVAAVQQVHNRKTEEYMRYNVSSMAVKLGEMQAQLIRLDSLGERISR
                     LSGVKPAKPEAIDKSGQGGPLVYSVQPHAADELQRDIDRLAEIVEQRSETLTELESQL
                     MERRIKNALLPTMVPIKAEHVGSTFGYRVDPIAGVRAMHEGIDFVADKGTRVIASAGG
                     VVQAAEYHPQYGNMIEIDHGNEFSSRYAHLSKIDVKAGQIVKRGQAIGASGNTGRSTG
                     PHLHFEVRFKGVAQNPARFVRQSTQMALNSPLAARPVPLPEKRIAPPANTANTPNTRA
                     TATFSAPR"
     gene            1831..2265
                     /locus_tag="PLD53_08170"
     CDS             1831..2265
                     /locus_tag="PLD53_08170"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRC60928.1"
                     /translation="MQKSLENYLEAADGAGKVLAHAKLLMKLTHLYQEIAPAHLGQTS
                     SLANFKSGTIVIHAVSGAAAAKLRQLAPTLVDGFCKRGIECNGVQIKVQARKISIQSM
                     TSAQKPLGSRARRELEGLRDSLPNAPLRTALETLLARAATKE"
     gene            complement(2252..2440)
                     /locus_tag="PLD53_08175"
     CDS             complement(2252..2440)
                     /locus_tag="PLD53_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60929.1"
                     /translation="MWLLRLLAVLTVISVAIGVVLFFLTRDQRYLQFAWRLFRYALIV
                     ALLVFGLLILERVAVIPL"
     gene            complement(2458..3399)
                     /gene="lpxC"
                     /locus_tag="PLD53_08180"
     CDS             complement(2458..3399)
                     /gene="lpxC"
                     /locus_tag="PLD53_08180"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRC60930.1"
                     /translation="MLKQRSLKSLIRASGVGLHSGVKVNMVMRPAAPDTGIVFRRIDL
                     DPVVDFRASALTVGDTRMCSCLEQEGPQGKIRVGTIEHLMSAFAGLGIDNAFVDLDAA
                     ELPILDGSASPFVFLIQSAGIEEQPVAKKFIRVKRPIEVREADPSGEKWARFEPYDGF
                     RLTFSILFNHPAIDRTGQEVTVDFAEHSYVREVARARTFGFMQEVEWLRENGLAQGGG
                     LDNAVVLDEYRVLNADGLRYADEFVKHKVLDAIGDLYLLGHPLLAAFTAHKSGHALNN
                     ALARELLANPDAWEYVSFEDQAQMPQAIERWMALRAT"
     gene            complement(3579..4760)
                     /gene="ftsZ"
                     /locus_tag="PLD53_08185"
     CDS             complement(3579..4760)
                     /gene="ftsZ"
                     /locus_tag="PLD53_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRC60931.1"
                     /translation="MFEIIEKEEATSDWGTVIKVIGIGGAGGNAVDHMIREGVMGVEF
                     VAANTDSQALKRSVAHKKIRLGKTGLGAGAKPEAGRSAAVEERDQIAESLKGAHMVFI
                     TAGMGGGTGTGAGPIIAEVARELGILTVAVVTKPFAFEGKRLKVAEEGIAELQKHVDS
                     LIVILNDKLMDVLGEDVSMDEAFKAADNVLRNAVGGIAEIINFPGLVNVDFEDVRTVM
                     GEMGMAMMGSANASGVDRARIAAEQAVASPLLEGINLSGAKGVLVNITSSRGLKMKEV
                     NEVMNTVREFAADDAHIIFGAVYDEAMDEEIRVTVVATGLGQAQAKRQSFEVINTPVM
                     KANGTNGGFAQEGTTGVDYGQLDAIPAVVRKGRNATVEALANSGVDRYDIPAFLRKQA
                     D"
     gene            complement(4799..>5802)
                     /gene="ftsA"
                     /locus_tag="PLD53_08190"
     CDS             complement(4799..>5802)
                     /gene="ftsA"
                     /locus_tag="PLD53_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRC60932.1"
                     /translation="DVYTGIAGSHIKSFNSNGMVAIKDKEVTPMDVERVIETARAMPI
                     PADQEILHILTQEFIIDDQDGIREPIGMSGFRLEVKVHIVTGAISAAQNIVKCVRRCG
                     LEVNDLVLQPLASSYAVLSEDEKDLGICLIDIGGGTTDLAIWTQGAIRHTSVIPIAGD
                     QITNDIAMALRTPTREAEDIKRKYGCALSDLADSEAVLDVAGVDDRPSRKLSRRALAD
                     VIQPRVEELFELIQAELRRSGFEEVLSSGIVLTGGSSVMPGMIELGEEVFHMPVRLGI
                     PKYHGALADVVQAPRFATASGLLIEAQTQRKRGLKVRERRDVKQVFGRMKSWLEKNF"
BASE COUNT         1295 a         1774 c         1521 g         1212 t
ORIGIN      
        1 cggagtgcgc gcctcgtcga tcaggatcga gtcgacctcg tcgacgatgg catagctcag
       61 tttgcgttgc acgcgctccc tggtcgtgta gaccatgttg tcgcgcaagt agtcgaagcc
      121 gaattcgttg ttggtaccgt aagtgatatc ggctgcatag gctagctgct tgtcatcatg
      181 cgccatctgc gacagattga cgccgaccgt cagcccgagg aaacgatgaa gacgccccat
      241 ccactcggaa tcgcgactgg ccagataatc gttcacggta atgatatgca cgcccttgcc
      301 ggcaagcgca ttgaggtagg ccggcagggt agcgaccagc gttttgcctt caccggttcg
      361 catttcggag atcttgccat catgcagcac catgccaccg accaattgga catcgaagtg
      421 acgcatgccg agcgaacgct tgcctgcctc acgaacaacg gcaaaggcct ccggcaacaa
      481 cgcatcaagc tcttccccat tggccacgcg ggccttgaac tcggccgtct tgccacgcag
      541 gtcatcgtct gaaagcgcgc tgatgcctgc ttccagcgca ttgatcttac ggatggccgc
      601 agaatactgc ttgatcagcc gatcattgcg gctgccgaaa aatttcttga gtaggccggg
      661 aatcattctg ggaacagtcg aagcacataa aggatgggat tctatcacgc gcaacccatg
      721 cagcggttcc ggaaaaactc cccggaatgc cggccatttc ggactcccgg atgattcctt
      781 atcgaggcgc gctgaaggtg gcagtggccc gtgtattcgg cgtgttcgct gtattcgccg
      841 ggggcgctat tcttttttca ggcagtggaa ccggccttgc agccagcggg gaattgagcg
      901 ccatctgcgt gctctgacga acgaaacgcg ccgggttttg cgcaacgcct ttgaagcgca
      961 cttcgaaatg caggtgcggg cctgtggagc gacccgtatt gccgctggcg ccgatggcct
     1021 gaccgcgctt tacgatctgc ccggccttga catcgatctt cgaaagatgg gcatagcgcg
     1081 aggagaactc attgccatgg tcaatctcaa tcatgtttcc gtactgcgga tgatactcgg
     1141 ccgcctgaac cacaccccct gcagaagcaa tgacgcgcgt ccccttgtca gcgacaaaat
     1201 cgatcccttc gtgcatggcc ctgacgccgg cgatcggatc gacacgatag ccaaatgtcg
     1261 aacccacatg ctcggctttg attggcacca ttgtcggcag cagtgcgttc ttgatgcgcc
     1321 gttccatgag ttgcgactca agctcagtca gtgtttcgct gcgttgctcg acgatctcgg
     1381 ccaacctgtc gatgtcgcgt tgaagttcat cggccgcatg cggctgaact gaatagacga
     1441 gcggcccacc ctgaccggat ttgtcgattg cctcgggctt tgctggcttt acacccgaga
     1501 gcctggatat ccgctccccg agcgaatcga ggcgaatcaa ttgcgcctgc atctcaccca
     1561 gcttgaccgc catcgaagaa acgttgtacc gcatgtactc ttcggtcttg cgattgtgta
     1621 cctgctgcac agccgccacg agttcggcag caaaaggcat gttaaaacga acaccgaccc
     1681 acgaaaacag gaacgaggtg gcaaaggcca ggatgatgac gcctgaaagc aacgccaaca
     1741 aggtgcggta cgatagattc aatgtcttgg cggttgccag tcgattggag acgagaataa
     1801 tatgcaagtt gctcctcctc gatgttaccg atgcaaaaat ccctcgaaaa ctatctggaa
     1861 gcagccgatg gtgccggaaa agtcctggca cacgcgaaac tgctcatgaa actgacgcat
     1921 ctgtatcaag agattgcgcc ggcacacctg ggccagacaa gcagcctggc caatttcaag
     1981 tcaggaacta ttgttattca cgccgttagc ggcgcggccg ctgccaagtt gcgtcagttg
     2041 gcgcccacgc tggtcgacgg tttctgcaaa cggggaattg agtgtaacgg cgttcaaatc
     2101 aaagtgcaag ccagaaaaat aagcatacaa tcaatgacat ccgcacagaa accattggga
     2161 tcaagggcca ggcgggagct tgaaggcttg cgcgactcgc tcccgaacgc cccgttacgg
     2221 actgctctcg aaactttgct cgcgcgcgca gctacaaagg aatgactgcg acccgttcca
     2281 gaatcagcag gccaaacacg agcaatgcaa caatcaaggc atagcggaac aggcgccaag
     2341 cgaactgaag atacctttga tcccgcgtga gaaaaaacag taccacgcca attgccactg
     2401 aaataaccgt caatacagcc agcaaacgta gcagccacac ggttcgtgac tccgaaacta
     2461 agtggctcgc aatgccatcc agcgctctat ggcctgaggc atctgcgcct gatcctcgaa
     2521 ggatacatac tcccaggcgt ctgggttggc cagcagctca cgggccagcg cgttgttcaa
     2581 ggcatggccc gacttgtgtg cggtaaaggc ggccaggagc ggatggccca gcagataaag
     2641 atcgccgatc gcgtcgagca ccttgtgttt gacgaattcg tcggcatatc gaagtccgtc
     2701 ggcattcagc acgcgatact cgtcgagcac cacggcgttg tccagaccac cgccctgcgc
     2761 cagaccgttc tcgcgcaacc actcaacctc ctgcatgaat ccaaaggtac gtgcgcgcgc
     2821 cacttccctc acatacgaat gctcggcgaa atcaacggtc acctcctgtc cggtacggtc
     2881 gattgcagga tggttgaaca ggatggagaa ggtcagcctg aatccgtcgt agggctcgaa
     2941 acgagcccat ttttctcccg aaggatcagc ctcgcgaact tcaatcgggc gttttacacg
     3001 aatgaatttc ttggcgaccg gctgttcttc gatacctgca gactgaatca gaaagacgaa
     3061 gggcgaagcc gacccgtcaa gaatcggcag ctcggccgca tcaagatcaa caaacgcatt
     3121 gtcgataccc aagccggcaa aggccgacat caggtgctca atggttccga cccggatttt
     3181 cccctgcggt ccctcctgct caagacacga gcacatgcgt gtatcgccga ccgtgagcgc
     3241 cgacgcccga aaatcgacca ctggatcaag atcaatacgc cgaaagacga tacccgtatc
     3301 gggcgctgcc ggacgcatta ccatattgac cttgacgccg gaatgcaggc caactccgga
     3361 agcgcggatc aaagatttta agctgcgttg tttaagcatc tatttgataa attggaaaat
     3421 ttcacgattg tatcacaagc gcgtcctgca tttggctgac cgaagcatct cccggaatca
     3481 cgcgcgacga caagagcctg gttgccaacc cactgccagc aggggaaaag ttccccggaa
     3541 ttcgaaagct tgcaggcagt gggtgtgcat ccccattgtc agtccgcctg ttttctgaga
     3601 aatgccggaa tatcgtagcg atcgacgccg ctattggcca atgcttcgac ggtcgcgtta
     3661 cgccccttgc gaacaacggc tgggatcgcg tccagttgac cataatcgac gccggtcgtg
     3721 ccttcttgtg caaaaccgcc attggtgcca ttggctttca tgactggcgt attgatcact
     3781 tcgaagctct gcctcttggc ctgagcctga cccaagccgg tggcgacgac cgtcacgcga
     3841 atttcctcat ccatcgcctc atcgtaaact gcgccaaaga taatgtgcgc gtcgtcggcg
     3901 gcaaactcgc gaacggtatt catcacctca ttgacttcct tcatcttcag gccccgtgac
     3961 gaagtgatgt tcaccaggac gcccttggcg ccggacagat tgattccttc gagcaagggc
     4021 gaagccaccg cctgctcggc agcaatgcgc gcacgatcga cccctgatgc gttggccgaa
     4081 cccatcatgg ccatacccat ctcgcccatc acggtacgta cgtcttcgaa gtcgacgttg
     4141 accagacccg ggaaattgat gatttcggcg attccgccca cggcgttgcg cagcacgtta
     4201 tccgccgcct tgaatgcttc gtccatcgac acatcttcac cgaggacatc catcaacttg
     4261 tcgttgagaa tgacgatcag cgaatcaacg tgcttctgca actccgcaat accttcctcc
     4321 gcgactttca gtcgctttcc ctcgaaagcg aagggcttgg tcaccacggc gacggtcaga
     4381 ataccgagct ctcgcgccac ctccgcaatg atcggcccgg cacctgttcc tgtgccgccg
     4441 cccatgccgg cggtaatgaa caccatatgt gctcccttta gcgactcggc aatctggtca
     4501 cgctcttcca ccgcggcact gcgtcccgcc tccggtttgg cacccgcacc cagacccgtc
     4561 ttgcccaagc ggatcttctt gtgcgccacg ctacgcttca gtgcctggga atcggtgttc
     4621 gcggcaacga actccacccc catcacgcct tcccgaatca tgtgatctac agcattgcca
     4681 ccggcgccgc caatgccgat caccttgatc acggtacccc aatcgctagt ggcttcttcc
     4741 ttttcgatga tttcaaacat ggctctctcc tccgcaaaaa actgataaaa aagtgtgact
     4801 aaaaattttt ctcaagccag gacttcatcc ggccgaatac ctgcttgaca tcccttctct
     4861 cacgcacctt caggccgcgt tttcgttgtg tttgcgcttc gatcaaaagt ccgctggcgg
     4921 tagcgaaacg cggtgcctgc acgacatcgg cgagcgctcc gtgatacttc ggtattccga
     4981 gtcgaaccgg catgtgaaag acttcttcgc caagctcgat cattcccggc atcaccgacg
     5041 aaccaccggt cagcacgatg ccggaggaca acacctcttc gaaacctgag cgacgcagtt
     5101 ccgcctggat cagctcaaac aactcctcga cgcgcggctg gataacgtcg gccagcgccc
     5161 gccgcgacaa tttgcgtgaa gggcggtcat caacacccgc cacatcgagc accgcttccg
     5221 aatccgccag atcggaaagc gcgcaaccat atttgcgctt gatgtcttcc gcctcgcgtg
     5281 taggcgtgcg caacgccatg gcgatgtcgt tggtgatctg gtcgccggca atcggaatga
     5341 ccgacgtgtg gcgaatggca ccctgggtcc agatcgccag atccgtcgta ccgcctccga
     5401 tatcgatgag acagataccc agatcctttt cgtcctcgga aagtacggcg taactcgatg
     5461 ccagcggttg cagcaccaga tcattcacct ccaggccaca acgacgcacg cacttgacga
     5521 tgttctgcgc ggcggaaatc gcaccggtca cgatatgcac cttcacttca aggcgaaaac
     5581 cgctcatccc gatcggttcg cgaatgccgt cctgatcgtc gatgatgaat tcctgcgtca
     5641 ggatgtgcag gatttcctga tccgccggaa tcggcattgc gcgcgccgtt tcgatgacgc
     5701 gctcgacgtc catcggcgtc acttccttgt ccttgatggc gaccatcccg ttcgagttga
     5761 agctcttgat gtggctgccg gcaatcccgg tataaacatc ct
//
LOCUS       DAORPM010000154         5800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3807_length_5800_cov_8.172498, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000154 DAORPM010000000
VERSION     DAORPM010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3807_length_5800_cov_8.172498"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..2304
                     /locus_tag="PLD53_08195"
     CDS             280..2304
                     /locus_tag="PLD53_08195"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRC60933.1"
                     /translation="MRLTVHYGLTIFLSAFLLFQVQPLIGKMILPWFGGSASVWTTCM
                     LFFQLVLLLGYFYSHWVVRFLSPSRQSLVHGALLLISLLLIPISPSADWKPTGAENPT
                     LRILGLLTVSIGLPYFVLSTTGPLLQAWFARERSGLVPYRLFALSNFGSMLALLAYPI
                     AVEPVFPTRGQSWLWSGLFACFVVSCALLAWRGRKGKPIAVQHHEEGPAPRWTDMVLW
                     AALAACPSILMVADTSFLTTNIAPIPMLWVLPLALYLLSFILSFERHGWYQRKLFLPL
                     LVVGLAALTYLPMLGLSEIPLYLSMTINLGAFFVACMVCHGELARLQPHPSHLTGYYL
                     MLAVGGVVGGFFVGIIAPYWFNSNYELSIGILLTGVVAAIAVIPKANFPSPLWRKASI
                     ASTVFLLLGLGWIQVDEHLGEISGSEVTVRNFYGTLQVFANPEGDYRSMLHGQIIHGR
                     QHLAKDKLDLPTTYYSAEGGAGRAMQIKAASGPLRVGVVGLGVGTLVSYGRKEDYFRL
                     YEIDPLVIDIARDNFSYLSRTAASTEIVLGDARLQLELEPAQQFDILVVDAFSGDSVP
                     VHLLTLEAFAQYFRHLKPDGVLAVHITNRFLDLQPVVKSAADHFDKDIRLVSFEGDRE
                     RLIFRSRWALISADRAFFNKPQLIDATPIAARRNFQPWKDDYSSIFSILM"
     gene            complement(2357..2788)
                     /locus_tag="PLD53_08200"
     CDS             complement(2357..2788)
                     /locus_tag="PLD53_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRC60934.1"
                     /translation="MKQVFGTRNGWAARCLVAMMLGYAVLVPLASADSDAVVREAGGL
                     SYVSGGVGEESLDRMKVLVTDFNLKLVLALNTGDYLSDVRVAIADSKGKTLVDTTSEG
                     PWFLAKLPAGTYQIVATYAGTEVKRQVTVGPERLNTVHLRW"
     gene            2926..4506
                     /locus_tag="PLD53_08205"
     CDS             2926..4506
                     /locus_tag="PLD53_08205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRC60935.1"
                     /translation="MTARKAVKQTRRRSIAAAPDAGASAQHASDTPFSNGMTGELRLR
                     AEALALKKAAKSQKRLEGLTPEAIRKTLFELSVHQIELEMQNETLRQSQVLLDTARAR
                     YFDFYDLAPVGYCTVCTQLLITEANLFAASLLGVTRSVLVKQPFLRFIHGNDQGVFMQ
                     FCKQIAETGKSQTCTLRLMKPDQSLFWVQLNAITLPDRDRAPEIHMVLNDIGELKRTE
                     EALRAQEEFFHLIAENIGDFIAVLDLDGRRIYNSPSYRQFFGSPKKLRGTDSFTVIHP
                     DDRERVKQVFRETVQTGIGQQIVYRLQLADGSSHTMESSGSVIRDNEGRIARVVVVSR
                     DITERKRMEEQVRQLAFYDPLTQLPNRRLLSDRLNQAMAASKRSACYGAVMFLDLDNF
                     KLLNDTQGHVVGDLLLVEAAKRLKSCVREMDTVARFGGDEFVLMISELDADKTESTVQ
                     VGVIAEKIFSALSQPFVLNTSSEREPETTIEHRCSASVGVALFVNQEASQDDILKWAD
                     AAMYRAKEAGRNLVRFHE"
     gene            complement(4539..>5800)
                     /locus_tag="PLD53_08210"
     CDS             complement(4539..>5800)
                     /locus_tag="PLD53_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRC60936.1"
                     /translation="INPNPITETWRNIGFARENRTVFLSILGISWFWLFGALLLAQFP
                     AYAKNVLGGTVTCVTLLLGIFTLGIGIGSLLCERLSSKQVEIGLVPFGSIGLTVFGLD
                     LAFASPATPPASALSIMPFLMAEGTIRILFDLFAIGLFGGFFIVPLYALMQIRSEASH
                     RARIIAANNIVNALFMVVGAGAAAVLLSSGLSIPMLFAVAAICNAAVALYIYGLVPEF
                     LLRFIVWLLVHSVYRLEKKDLDRIPESGPALIICNHVSYVDALVITAACHRPIRFVMD
                     HHIFRWPILSFVFKAGRAIPIASARDDPVLLEKAFDEVSAALAAGELVGIFPEGQITS
                     DGELCPFRPGISRILERNPVQVVPVALSGLWGSIFSRRDGPAMSALWKARPFRRIAIA
                     AGEPVNASQALPDSLRADVLALRGELR"
BASE COUNT         1178 a         1707 c         1699 g         1216 t
ORIGIN      
        1 ttgaacacta cgaagatgct gtctcgaaca ctacggggag tttttcaaca gaatcggtcg
       61 atatgggtat ttgccaacgc aaattgccgc tcgacgactg actatttcat cacgaataca
      121 acattcgcct gtcctcttcg taggtgcgaa tccagctcac aaagattctg atgctccagc
      181 atgtttttct actggataat ccgggtgctc ggcaaacgta gttccagata aacaggccat
      241 ccggcgaaac ctccaactcc tatctccatg aaaattgact tgcgactcac ggtccactac
      301 ggactgacga tctttctcag cgcgttcctt ctgttccagg tacaacccct gattggcaaa
      361 atgatcttgc cgtggtttgg cgggtcggcg tcggtgtgga ccacctgcat gctgttcttc
      421 cagttggttt tgctgcttgg ctatttctac tcgcattggg tcgtgcgatt cctgtcgcct
      481 tcgcggcaaa gcttggtaca cggcgccctg ttgctgatca gcctgctgct gattccgatc
      541 agcccgagcg ccgactggaa gccgaccggc gccgagaacc cgacactgcg catcctgggc
      601 ctgctgaccg tttcgatcgg cctgccttac ttcgtgctgt ccacgaccgg cccgctgctg
      661 caggcctggt tcgcccgcga acgatccggt ctggtgccgt accggctctt cgccttgtcg
      721 aatttcggtt cgatgctggc gctattggcg tatccgatcg ccgtcgagcc agtgttcccg
      781 acgcgcgggc aatcctggct ttggtccggg ctgtttgcgt gcttcgttgt ttcctgcgcg
      841 ctgctagcct ggcgcgggcg caaggggaaa cccatcgccg tgcagcatca tgaggaaggg
      901 cccgcaccgc gctggacgga catggttctg tgggcggctc tggccgcgtg cccgtcgatt
      961 ctgatggtgg ccgataccag tttcctgacc accaacattg caccgattcc gatgctctgg
     1021 gtgctgcctt tggcgctgta tctgctctcg ttcatcctga gtttcgagcg ccacggctgg
     1081 tatcagcgca agctgttcct tccgctgctg gtggtcggcc ttgcggcgct cacctacctg
     1141 ccgatgctgg gcttgagcga gatccccctt tacctttcca tgaccatcaa tctcggggcc
     1201 ttctttgtgg catgcatggt gtgtcacggc gaactcgccc gactgcaacc gcaccccagc
     1261 catttgaccg ggtattacct gatgctggcg gtcggcggtg tcgtgggcgg attcttcgtc
     1321 ggaatcatcg cgccttactg gttcaacagc aattacgaac tgtcgatcgg catcttgctc
     1381 accggggttg tcgccgccat cgcggtcatt ccgaaagcaa actttccaag cccgctctgg
     1441 cgcaaagcaa gtattgcctc gaccgtgttc ctgttgctgg gtttgggctg gatccaggtc
     1501 gacgagcatc tcggcgagat cagcggctcg gaagtcacgg tgcgcaactt ctacggcaca
     1561 ttgcaggtct ttgccaaccc cgagggcgac taccgcagca tgctgcacgg gcagatcatt
     1621 cacggccgtc agcatctcgc gaaggacaaa ctggatttgc cgacgaccta ctacagtgcc
     1681 gaaggcggcg ccggcagggc gatgcagatc aaggcggcca gcggcccgtt gcgcgtcggc
     1741 gtggtgggac tcggtgtcgg cacgctggtc agctacgggc gcaaggagga ttacttccgg
     1801 ctttacgaaa tcgatccgct ggtgatcgac attgcgcgtg acaacttcag ctatctttca
     1861 cgcaccgccg ccagcacgga aatcgtcctc ggcgacgccc gtctgcaact cgaactggag
     1921 ccagcgcagc agttcgatat tctggtcgtc gacgcctttt cgggcgattc ggttcccgtc
     1981 catctgctga cccttgaggc attcgcccag tatttcaggc atctgaagcc ggatggcgtg
     2041 ctggccgtgc atatcaccaa ccgctttctc gatttgcaac cggtggtgaa atccgccgcc
     2101 gaccacttcg acaaggacat tcgtctggtc agtttcgagg gcgatcgcga acgcctcatc
     2161 ttccgttcgc gttgggcgct gatcagcgcg gatcgcgcct ttttcaacaa gcctcagctc
     2221 atcgatgcga cgccgattgc cgcacggcgc aactttcagc cctggaaaga cgattacagc
     2281 agcattttct cgatcctgat gtaacgaata ccggatggcc tgtcgggatg gcgcctgatc
     2341 gctctcctta ccccgctcac cagcgtaaat ggacggtgtt cagtctttcc gggccgacgg
     2401 taacctgccg cttgacctcc gtgccagcgt aggtcgcaac aatctggtac gtgccagcag
     2461 gcagcttcgc caggaaccag gggccttccg acgtggtgtc taccagcgtc ttgcccttgc
     2521 tgtcggcgat ggcgaccctg acatcgctca ggtaatcgcc agtattcagc gcaagcacca
     2581 gcttgaggtt gaagtcggtg acgagcacct tcatccggtc gagcgattcc tcgccaacgc
     2641 ctcccgacac ataactcaaa ccgccggctt cgcggacgac ggcgtcgctg tcggcgctgg
     2701 ccaagggaac aagtacggca tagcccagca tcatggccac gaggcagcgt gcagcccatc
     2761 catttctcgt tccaaaaacc tgtttcatag ctggttcctc caataggtgg tgaaacaatt
     2821 cggcaatcga gctctgggct cgtggccgaa cgattgcccc aaactgggaa tcttcggaaa
     2881 ttgtactatg ctgaaaagcg gtgccaagac gcgctgaggg tttttatgac cgccagaaag
     2941 gcagtgaagc aaaccaggcg ccgctccatt gctgctgcac ctgacgccgg tgcttccgcg
     3001 cagcatgcca gtgacacccc cttttcgaat gggatgactg gggaactccg cctgcgtgcc
     3061 gaagcgcttg ctctcaagaa agcggcaaaa tcgcaaaagc gccttgaagg gttgacgcct
     3121 gaggcaatcc ggaagacgtt gttcgaattg tccgtgcatc agatcgagct ggaaatgcag
     3181 aacgagacgc tgcgccagtc gcaggtgttg ctggacaccg ctcgcgcgcg ctatttcgat
     3241 ttctatgatc tggcgcctgt gggctactgc actgtctgca cgcagctgtt gatcaccgag
     3301 gccaaccttt ttgccgccag cttgctgggt gtgacgcgta gcgtgctggt caagcagccg
     3361 ttcttgcgct ttattcacgg caatgaccag ggcgtgttta tgcagttctg caaacagatc
     3421 gccgaaaccg gtaagtcgca gacctgcacg ttgagattga tgaagccgga ccaatcgcta
     3481 ttctgggtac aactgaacgc cattacctta ccggatcgcg accgggcacc ggaaatccat
     3541 atggtgctga atgacatcgg cgagctcaaa cgcactgaag aagccttgcg tgcacaggag
     3601 gagttctttc atctgatcgc ggaaaatatc ggcgatttca ttgccgtcct tgatctggac
     3661 gggaggcgca tctacaacag cccctcgtac aggcagttct tcggttcgcc aaagaagttg
     3721 cgcggcaccg attccttcac cgtgatccat ccggacgacc gcgagcgcgt caaacaggtt
     3781 ttcagggaga ccgtgcaaac cggtatcggc caacaaatcg tttatcgcct gcagttggcc
     3841 gatggcagta gccacaccat ggagtcgagc ggcagcgtaa ttcgggacaa cgagggccgg
     3901 attgcacggg tggtcgtcgt gtcgcgcgac atcaccgagc gcaagcggat ggaagagcag
     3961 gtgcgccagc tagcgtttta cgatccgctc acgcagttgc cgaaccgtcg cctgctttcc
     4021 gaccgcctga accaggccat ggcggccagt aaacgcagcg cctgctatgg cgccgtgatg
     4081 ttccttgatc tggataattt caagttgctg aatgacacgc agggccatgt cgttggtgat
     4141 ttgctgctgg tcgaggcggc aaaacgtctc aagagctgtg tgcgcgaaat ggacaccgtg
     4201 gcgcgttttg gcggcgatga atttgtgttg atgatcagtg agctggacgc cgacaagacc
     4261 gagtctaccg ttcaggtcgg cgtcatcgcc gagaaaatat tctccgccct gtcacaaccc
     4321 tttgtgctga atacgagttc cgaacgggag cccgaaacca ccatcgagca tcgctgttcg
     4381 gcgagcgtcg gcgtagccct gtttgtcaat caggaagcca gccaggacga catcctcaag
     4441 tgggctgacg ccgcgatgta tcgggccaaa gaggccggac gcaatctggt ccggtttcac
     4501 gagtaaggtt tctggtcggg ctgccaacag gcaccgggtc acctgagctc tccccgcagt
     4561 gccaggacat cggccctgag cgaatccgga agcgcctggc tggcattgac cggctcgccg
     4621 gccgcaatgg cgattcttct gaagggtcgt gccttccaca gggcgctcat cgccgggcca
     4681 tcgcgtcggg agaaaatgct gccccaaagc ccggacagcg ctacgggaac aacctgcacc
     4741 ggattgcgtt cgagaatacg gctgatcccg gggcggaatg ggcaaagctc gccatcggaa
     4801 gtgatctgtc cttccgggaa tattcccacc agctcgcctg cggccaatgc cgcggaaacc
     4861 tcgtcgaacg ctttttccag cagcaccgga tcgtcccggg ccgaagcaat cgggatggcg
     4921 cgtccggcct tgaaaacgaa cgacagaatg ggccagcgaa agatgtggtg gtccatgacg
     4981 aagcgaatcg gacggtggca tgcggccgtg atgacaagag cgtcaacata gctcacgtga
     5041 ttgcagatga tcagcgcggg gccgctctcg ggaatgcgat caaggtcctt cttttcgaga
     5101 cggtacaccg aatggaccag cagccagaca atgaatcgca ggaggaattc tggcaccagt
     5161 ccgtagatgt agagcgccac cgccgcgttg cagatcgccg caacggcaaa cagcatgggg
     5221 atgctcagac cgctggacag tagcacggca gcggcaccgg cgccgacgac catgaacaac
     5281 gcattgacga tattgttcgc ggcaatgatt cgcgcccggt ggcttgcctc cgagcgaatc
     5341 tgcattagcg cataaagcgg gacgatgaag aaaccgccaa aaagaccaat ggcgaagagg
     5401 tcgaacagaa tgcgaattgt tccctcggcc atcaggaacg gcatgatcga caaggcgctg
     5461 gcgggtggcg tcgccgggga ggcaaaggcc agatcaaggc caaatacagt gaggccgatt
     5521 gagccgaaag gtaccagacc gatctcgacc tgtttcgacg acaggcgttc gcagagcagt
     5581 gagccgatgc cgatacccag cgtgaagatg ccgaggagca gggtgacgca cgtcaccgtg
     5641 ccaccgagta cgttcttggc ataggcggga aattgcgcga ggagcagcgc gccgaacagc
     5701 cagaaccatg agattccgag gatcgacaaa aaaaccgtgc ggttctcgcg ggcgaaaccg
     5761 atattgcgcc aggtttcggt aatcggattc ggattgatct
//
LOCUS       DAORPM010000155         5778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3826_length_5778_cov_9.539402, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000155 DAORPM010000000
VERSION     DAORPM010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3826_length_5778_cov_9.539402"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2417
                     /locus_tag="PLD53_08215"
     CDS             <1..2417
                     /locus_tag="PLD53_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC60937.1"
                     /translation="TVLFVVISQLKINVTNAYAGSLAWSNFFARLTHSHPGRVVWVVF
                     NILIALLLMELDVFQALGQILGLYSNIAISWMAAVVADLVINKPLGLSPPHIEFKRSH
                     LYDINPVGIGAMCLASLLSIAAFSGLLGPDVQPYASFIALSAAFVAAPLIAWITGGRY
                     YLVANKTVAAAGMQRCCICEREYESEDTAHCPAYRGTICSLCCSLDARCDDLCKPGAN
                     LAAQWNGTLRRILPAAAIPYLETGLGHYLLLMVCIVPLLAGLLGLLYTHETLALGPEA
                     SELIPHLQQTFIKAFSALLLFAGVGAWWMVLTHKSRQVAQEESNRQTRLLMQEIDSHQ
                     RTDEQLQKAKLAADQANQAKSRYITAISHELRTPLNSILGYAQILAGDEAIPAHRRQA
                     VGVIRKSGDHLLSVIEGTLDIARIEGGKLTLEVKALDFPEFLQQIVGMFELQARNKGL
                     SFSYEPVGELPGVVRVDQRRLQQILINVLGNAVKFTVHGGVNFRVECRREMAIFDIRD
                     SGPGIPPEDLERIFEPFVRGSQTQAGGSGVGLTIARMLTDLMGGEMTVSSIPGEGSSF
                     RIRLFLPQVHSAQAARDLPRTNRIGYVGIRRRILVVDNEKVDRDMLQSVLDPLGFQVE
                     QAASGHECLQVLPRFAPHLVFMDLAMPGIDGWETIRRIRQQNLTDAHIAIISANAFDR
                     GLENDAGISNDDFIVKPLRVDELLDWIGRKLSLEWVSAESPVIPPTRVVPKPAQLIPP
                     GQEHLSALDELVGLGYVRGILNKLGEIERLDPAHGEFVRVLRDLARQFQFDAMKEILG
                     KGRDVE"
     gene            2407..3342
                     /locus_tag="PLD53_08220"
     CDS             2407..3342
                     /locus_tag="PLD53_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC60938.1"
                     /translation="MLNDPPLDRTISDIVLIVDDIPENLSFLHDALDESGYTVLVATN
                     GESALQRARQSLPHVILLDAMMPGMDGFEVARRLKADFATQHIPIVFMTGLTETEHVV
                     AAFAAGGADYVTKPIRAAEVLARIAAHMQNAHQIKQARTALDAFGQATVAIRAADGKL
                     VWQTPLARKLLKDYFSTGEEQTPQRLLAWIADAESARRDGREPAALLLAEDRRRLLAS
                     FHDQTGDQEWLVVLREENDATAIDSLIAAFRLTQREAEVLYWVTLGKTSKDIGDILGS
                     SPRTVNKHLEHVFEKLGVETRTAAANLAIGKIRGS"
     gene            complement(3385..3867)
                     /locus_tag="PLD53_08225"
     CDS             complement(3385..3867)
                     /locus_tag="PLD53_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRC60939.1"
                     /translation="MELLLWRHAEAVEGTPDLKRKLTVRGEKQARQMAEWLGKHAPKK
                     LRILVSPALRCQQTAQALGLPFETDKRLGCESNVPELLAATGWPNWGAESAASGAVLV
                     VGHQPTLGQTAALLLSGTESDWSIKKGAVWWFSNRTRQGETQNVLRAVIPPDFVRQLA
                     "
     gene            complement(3938..4627)
                     /locus_tag="PLD53_08230"
     CDS             complement(3938..4627)
                     /locus_tag="PLD53_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC60940.1"
                     /translation="MPPSQGVNIEALLSRVPLFNDLEADEVARIARGTREINALRGDI
                     LFHKGDSPTGFYLIVYGQVKLAFTSSQGGEKVVDILNQGQTFGEAVMFMDKPCMVYAQ
                     ALSDSQLLHISKNAIIDELERDPKLGRKMIAGLSMRLHHLLIDVESYSLHSGRQRIIG
                     YLLRDNVKSDAKSLTVTLPTNKGVIASRLNLTQEHFSRILHELSDKGLIVVEGRKIGI
                     PDVEKLRCHEI"
     gene            complement(4707..5348)
                     /gene="ureG"
                     /locus_tag="PLD53_08235"
     CDS             complement(4707..5348)
                     /gene="ureG"
                     /locus_tag="PLD53_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HRC60941.1"
                     /translation="MNTLNSNSASTTHNAPLRVGIGGPVGSGKTALTLALCRALREQI
                     EMAVVTNDIYTAEDAKFLVDHAALEPTRIIGVETGGCPHTAIREDASINLEAIDRLQR
                     TFPQVQLILVESGGDNLAATFSPELSDLTLYVIDVAAGEKIPRKGGPGITKSDLLIIN
                     KIDLAPMVGASLDVMARDARTQRGERPFVFTNLKTGEGLEPVIAFIRERGMLI"
     gene            complement(5355..>5778)
                     /locus_tag="PLD53_08240"
     CDS             complement(5355..>5778)
                     /locus_tag="PLD53_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HRC60942.1"
                     /translation="SREGAELRAETLQMGFSLNRLLADLRDPTLSGVQATLTSLPEIS
                     FPVAWTGIAAAWNIDPPAACAAYLWSWAENQVMAALKAAPLGQAAGQRLLAELGSRIP
                     AVVRGAQNLPPAAWSNFTPAYAICNVRHETQYSRLFRS"
BASE COUNT         1161 a         1733 c         1689 g         1195 t
ORIGIN      
        1 caaccgttct ttttgttgtc atttcacagc tcaagatcaa tgtaaccaat gcctatgccg
       61 ggtcgctggc ctggtcgaat ttttttgccc gcttgacgca cagtcatccc ggacgcgtcg
      121 tctgggttgt cttcaacatt ctgatcgcct tgctgctcat ggaactggat gttttccagg
      181 cgttgggcca aatcctcggc ctctattcaa atatcgccat ttcatggatg gcggccgtgg
      241 tcgccgacct cgtgatcaac aagccgcttg gcctctcgcc gccgcatatc gagttcaagc
      301 gcagccatct ctacgatatc aacccggtcg gtatcggcgc catgtgcctc gcctcgctgc
      361 tctcgatcgc agcgttcagc ggactgctcg gccctgatgt gcagccctat gcctccttca
      421 tcgcgctatc ggccgccttt gtcgccgcac ctctgattgc ctggataacc ggtggccgct
      481 attaccttgt tgccaacaag accgttgccg ccgccggcat gcagcgttgc tgcatttgcg
      541 aacgcgaata cgaaagcgag gacacggcgc actgcccggc ctaccgtggc acgatctgct
      601 cgctgtgctg ctcactcgat gcgcgctgtg atgatctgtg caaaccgggc gccaatctcg
      661 cagctcaatg gaacggcacg ctgcgtcgaa tcctgcccgc agcggcgatt ccctatcttg
      721 aaaccggact gggccattat ctgctgctga tggtgtgcat cgtccccctg cttgctggcc
      781 tgctcggtct gctttacacg cacgaaacgc tggcgctcgg cccggaagcc tctgaattga
      841 ttccgcacct gcaacagacc tttatcaaag ccttttcggc attgcttctc ttcgccggcg
      901 ttggcgcctg gtggatggtt ctgacccaca agagccgaca ggtcgcgcag gaggaatcga
      961 accggcagac acgtctgctg atgcaggaaa tcgactcgca ccagcgcacc gatgagcaat
     1021 tgcagaaggc caagctggcg gcggaccagg caaatcaggc caagagccgc tatatcactg
     1081 cgatcagcca cgagttgcgc acgcccctca acagtatttt gggttacgcg caaattctcg
     1141 ccggcgacga ggccatcccg gcacaccgtc ggcaagcggt cggcgtgatc aggaagagcg
     1201 gcgaccattt actgtcggtg atcgaaggca cgctcgatat cgcccgaatc gaaggcggca
     1261 aactgacact ggaagtcaag gcgctggact tcccggagtt cctgcaacag attgtcggca
     1321 tgttcgagtt gcaggcccgc aacaagggct tgagcttcag ttacgaacct gtcggtgaac
     1381 tcccaggcgt cgtgcgggtt gatcagcgac ggcttcagca aatcctgatc aacgtcctcg
     1441 gcaacgccgt caaattcacg gtgcatggcg gcgtcaattt ccgcgtcgaa tgcaggcgcg
     1501 aaatggccat cttcgatatc cgcgatagcg gccctggcat tccaccggaa gatctggaac
     1561 gtatttttga gccttttgtc cgtggtagcc aaacgcaggc tggcggaagc ggtgtcggac
     1621 tgaccattgc gcggatgctg accgacttga tgggcggcga aatgaccgtc agcagcatac
     1681 cgggtgaagg cagcagcttc cgcatccgac tattcctgcc tcaggtacac tcggcgcagg
     1741 cagcgcgcga tttgccacga accaatcgga tcggttatgt cggtattcgt cgccgtattc
     1801 tggtggtcga taacgagaag gttgatcgcg acatgctgca gagtgtcctt gaccctctgg
     1861 gtttccaggt tgaacaggcg gccagcgggc atgaatgcct gcaagtgctg ccccgcttcg
     1921 ccccgcatct ggtgttcatg gatctggcca tgcccggcat cgatggctgg gaaaccatcc
     1981 ggcgtattcg ccagcaaaat cttacggatg cccatatcgc catcatctcg gccaacgcct
     2041 ttgacagggg tcttgaaaac gatgccggca tcagcaacga cgacttcatc gtcaaaccgc
     2101 tgcgtgtcga tgaactgctc gactggatcg gccgcaagtt atcactggag tgggtcagcg
     2161 ccgaatcgcc ggtcatccct ccaacccgtg tcgtgcccaa gccagcccaa ctgatcccac
     2221 ccggacaaga gcatctttcg gctctcgacg aactggtcgg cctcgggtat gttcgcggca
     2281 tactgaacaa acttggtgaa atcgagcgac ttgaccctgc gcacggcgag tttgtgcgtg
     2341 tcctgcgcga tctggcccgt cagttccagt tcgacgccat gaaggaaatt ctcggaaagg
     2401 gacgcgatgt tgaatgaccc tccacttgac cgcaccatct ccgatatcgt tctgatcgtg
     2461 gacgatatcc cggagaacct ctccttcctg cacgacgccc tcgacgaatc gggctacacc
     2521 gtactcgtcg cgaccaatgg cgaatccgcc ctgcaacggg cgcgccaaag cctgccgcac
     2581 gtcattctgc tcgatgccat gatgccgggc atggacggct tcgaagttgc gcgacgcctg
     2641 aaagccgatt tcgccactca gcatattccg attgttttca tgaccggcct gaccgaaacg
     2701 gaacacgtcg tggctgcctt tgcggccggc ggtgccgact acgttaccaa gcccatacgc
     2761 gccgccgaag tgctggcccg catcgccgct cacatgcaga acgcccacca gataaagcag
     2821 gcccgaacgg ccctggatgc cttcggccag gctacggtgg ccattcgtgc cgccgacggc
     2881 aagctggtct ggcagacgcc cctggccaga aaactgctca aggactattt ctccaccggg
     2941 gaagaacaaa cgccgcagcg cctactggcc tggatcgccg acgccgagtc cgcccgtcgc
     3001 gacgggcgag agccagccgc actgctactc gccgaagaca gacggcgact gctcgcctcc
     3061 ttccacgacc agacgggtga ccaggagtgg ctggtcgtcc tgcgcgaaga aaatgatgcc
     3121 accgccatcg attccctgat cgccgctttc cggctcactc aacgtgaagc agaggtgctc
     3181 tattgggtaa cgctgggcaa gaccagcaag gacatcggcg acatactcgg cagcagtcca
     3241 cggacggtga acaaacacct ggagcacgtt ttcgaaaaac tcggcgtgga gacccgcacc
     3301 gcggcggcca atctggcgat cggcaagatc cggggaagct gaatgtggaa gcgggacgct
     3361 accctgttct cgtccagcgc ccgatcatgc caattggcgc acgaaatccg gaggtatgac
     3421 cgcccgcaaa acattctgcg tctcgccctg ccgcgtgcga ttgctgaacc accagacagc
     3481 gcctttcttg atcgaccagt cggattccgt tccggacaag agaagggcgg cagtctggcc
     3541 gagggtgggc tgatggccga caacaagtac cgcaccactg gctgcgcttt cagcacccca
     3601 gttcggccat ccggtcgcgg ccagtagttc ggggacgttg ctctcgcatc ccaaccgttt
     3661 gtcggtctcg aaaggaagtc cgagtgcttg cgccgtctgc tggcagcgaa gcgcagggct
     3721 gaccaggatg cgcagtttct ttggcgcgtg cttgccgagc cattcggcca tttgccgggc
     3781 ctgcttttcg ccacgcaccg taagcttgcg cttcaggtcg ggcgtgccct cgacagcttc
     3841 cgcgtgacgc cataacagga gttccatgtg agtttccgtt gacatcaagg acggcatgtt
     3901 ggcctgtgat tgtttcatgg gtatttccgg tcaaatttca aatttcgtgg cagcgcaatt
     3961 tctcgacatc gggaatgccg atcttccgcc cttcgacaac gatcaaaccc ttgtcggaga
     4021 gttcgtgcag gatgcgcgag aagtgttcct gcgtcaggtt gaggcgcgag gcaatcacgc
     4081 ccttgttggt gggcaaggtg acagtcagcg acttggcatc gctcttgaca ttgtcacgta
     4141 gcaggtaacc gatgatgcgc tgacgacccg agtgcagtga ataggattca acgtcgataa
     4201 gcaggtgatg aagtctcatc gagaggcccg cgatcatctt gcgaccgagc ttggggtccc
     4261 tttccaactc gtcgatgatg gcattcttcg agatatgcaa cagctgagaa tcgctcagcg
     4321 cctgcgcgta gaccatgcag ggtttgtcca tgaacattac ggcttcgcca aaagtctgcc
     4381 cctggttgag aatgtctaca accttctccc ccccttgcga cgaagtgaac gcgagtttta
     4441 cttgaccgta aacgatcagg taaaagccgg taggtgaatc gcccttgtga aacaggatgt
     4501 cgcctcgcaa ggcattgatc tcgcgcgttc cgcgcgcgat acgggcaact tcatccgctt
     4561 caaggtcgtt gaataggggc acacgcgaaa gtagtgcctc aatgttgaca ccctgacttg
     4621 ggggcatagg cgatatccaa aataacgatt tccgttgcgg caaatggtcg cacttgtcgg
     4681 agaatcaggc aagcgcgata ggcacttcag atcagcattc cacgctcacg aatgaaggcg
     4741 atgaccggtt cgagaccctc tcctgtcttg aggttggtaa agacaaaggg ccgctcgccg
     4801 cgttgcgtcc tggcgtcacg cgccatgacg tcgagcgacg caccaaccat cggagccaga
     4861 tcgattttgt tgatgatcag caaatccgac ttggtgattc ccggaccgcc cttgcgcggg
     4921 attttttctc cggcggcgac atcgatgacg tacagcgtca ggtccgaaag ttcgggcgaa
     4981 aacgtcgcgg cgaggttgtc gccgcccgat tcgaccagga tcagttgcac ctgcggaaaa
     5041 gtccgttgca ggcggtcaat ggcttccaga ttgatggatg cgtcctcgcg gatggccgta
     5101 tgtgggcagc cgccggtctc gacgccgatg attcgcgttg gctccagcgc ggcatggtcg
     5161 acgaggaact tggcgtcttc ggcggtgtag atgtcgttgg tcaccaccgc catttcaatc
     5221 tgttcgcgca aagcccggca cagcgccagg gtcaatgcgg tcttgccgga gccgacggga
     5281 ccgccgatgc cgacgcgcag gggagcgttg tgagttgtgg atgcggaatt tgaattgagc
     5341 gtgttcatgg atggtcaaga ccgaaaaaga cgtgaatact gtgtttcgtg gcggacatta
     5401 cagatggcat aggccggcgt gaagttggac cacgccgctg gcggcaggtt ttgcgcgcca
     5461 cgaacaacgg ccgggattct tgatccaagt tctgcgagca gccgctggcc ggcagcctgg
     5521 ccaagaggcg cggctttcag cgcggccatc acctgatttt cggcccacga ccacaggtag
     5581 gcggcgcagg ccgctggcgg atcgatgttc caggcagcgg caataccagt ccaggcgacg
     5641 gggaaggaaa tctcgggcaa cgatgtcagg gttgcttgta caccgctaag cgttgggtcg
     5701 cgcagatcgg ccagcagacg gttgagcgaa aaccccatct gaagagtttc ggcgcgcaac
     5761 tcggcgcctt cgcggctg
//
LOCUS       DAORPM010000156         5746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3859_length_5746_cov_10.536110, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000156 DAORPM010000000
VERSION     DAORPM010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3859_length_5746_cov_10.536110"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLD53_08245"
     CDS             complement(<1..599)
                     /locus_tag="PLD53_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRC60943.1"
                     /translation="MRFSLFRSFVLLSSLFLALFLSACASPQLRGTGDLGLVIERASG
                     SVILVDTSKRAPYAHIEGLGDLSHASAVYSRDGRYAYIFGRDGGLTKIDLLEARIVKR
                     VMQAGNSIGGSISQDGRIVVAQNYTPGGIKAFDAETLDLLSEVPAEYAPGKFSKVVGL
                     ADLSGNRFGYALFDGGEIWVSDFSDPKHPKTQRYPAGMQP"
     gene            759..>1180
                     /locus_tag="PLD53_08250"
     CDS             759..>1180
                     /locus_tag="PLD53_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRC60944.1"
                     /translation="MPNNRDALELLAPAKNAEFGIEAISHGADAVYIGGPAFGARVNA
                     GNSVADIERLASHAHRFNARVLVAINTLLKDDELEEARRLIWECYEAGADALIVQDMG
                     LLELDLPPIELHASTQTDIRTPEKARFLEDVGFSQIVLA"
     assembly_gap    1181..1280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1281..2459
                     /locus_tag="PLD53_08255"
     CDS             <1281..2459
                     /locus_tag="PLD53_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HRC60945.1"
                     /translation="FKIEGRLKDLSYVKNITAHYRRQLDDIIEDIPSYRRSSSGVTTL
                     FFTPQTEKTFNRGATDYFLNDRKSDIGAFDSPKFVGEKIGTVIGIGRDSIDVCSDAPL
                     HNGDGITCFDADLKLVGLRINRAQQVASGYRIFPTPGEGRFPTDLRIGETIYRNHDQE
                     FVRQLEKKSAERSIRVRMQFEETGDGFALHITDDEGVGAVAHVAHDKKAANNPELAMT
                     RLRENLGKLGNTIYRSETIDLAIDTAWFIPDSVLNALRRAAVEALDAARNAAYKQPER
                     RAAMVPPAPYPDDALSYLGNVLNAKARAFYEKHGVSVIAPAYECNAEKGEVSLMITKH
                     CLRYSFNLCPKQVKGIRPDPMILMNGNEKLTLRFDCKPCEMHVIGKLRKNRTIQLAIR
                     "
     gene            complement(2474..2677)
                     /locus_tag="PLD53_08260"
     CDS             complement(2474..2677)
                     /locus_tag="PLD53_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HRC60946.1"
                     /translation="MKDRLTELEIKTSLNEDLLEELNRTVYRQQQQIDLLQEQMRLLY
                     QQIKDNAEPDENSSLRDEIPPHY"
     gene            2836..3093
                     /locus_tag="PLD53_08265"
     CDS             2836..3093
                     /locus_tag="PLD53_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60947.1"
                     /translation="MSLEPCRACGHQVDTSALACPGCGATDPAKRISRQQHNARVFVI
                     QLIVCLSLLGFGGWYVWSTLVPVIKQFIAKPQAEQTQAGQQ"
     gene            complement(3130..4020)
                     /gene="ylqF"
                     /locus_tag="PLD53_08270"
     CDS             complement(3130..4020)
                     /gene="ylqF"
                     /locus_tag="PLD53_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HRC60948.1"
                     /translation="MAVQWYPGHMTSARKKAAETMASIDVVIEVLDARLPEASTNPLV
                     RELRLHRQRPCLKVLNKADLADPEATRSWIDFYNRQENVKAVALSSKNQGEVARIPAL
                     CQRLAPHRNDNIKPLRMMIMGIPNVGKSTLMNALVKRKIAKVGDEPAVTKSQQTHQIS
                     VRHSITDTPGLMWPKIEYPSDGLMLAASHAIGRNAVIDEEVAVFLAGLLLVRYPALLS
                     SRYGFVLDGVDAVAVVEGVAKKRSLILRGGGPDFEKASAVLVQDYREGKLGRISLETP
                     ETRRMMIGATNETPALSTGQ"
     gene            4116..4952
                     /locus_tag="PLD53_08275"
     CDS             4116..4952
                     /locus_tag="PLD53_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRC60949.1"
                     /translation="MKPVIPIPEIKPEQSIELLKELHILTREGKLNQDSRRKLKQVYH
                     LYHFIEPLLAELLAGNEAMTLADHGAGKSYLGFILFDLFFKNRTGGHIFGIETREELV
                     DKSRELAQRLQFGRMSFLNLSIEDSIRVATLPERIDMVTALHACDTATDDAIRFALCK
                     KARFIVLVPCCQAEVAAALRRNKNDSFSKTPLSELWRHPIHTREFGSQITNVLRCLQL
                     EACGYQIQVTELVGWEHSIKNELIIARKTGQVRKNAADRLEAILHELNLDELRERFMF
                     SG"
     gene            5001..5318
                     /locus_tag="PLD53_08280"
     CDS             5001..5318
                     /locus_tag="PLD53_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60950.1"
                     /translation="MDNWITRIAAALCAAGSTGLFWMFGVFIAVPWREGRMLALTKTE
                     LQVVGIPLVIGFAVAWGALHIFAISDRAANPKVYATIRWVVILIAIAAVIGGKAWTDA
                     RIA"
     gene            complement(5377..>5746)
                     /locus_tag="PLD53_08285"
     CDS             complement(5377..>5746)
                     /locus_tag="PLD53_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60951.1"
                     /translation="VDVGNRKGKAREMVYSDSWVRSGPEQEDSAMEAGSVSSSINTAS
                     ASALQAQVRARPPEQDEQTQQSQQSQQSQQAQGTQETQSNQSVEESRAADRAQSDVEG
                     SRPTVNANGQTVGTRVNTTA"
BASE COUNT         1248 a         1686 c         1527 g         1185 t
ORIGIN      
        1 ggctgcatgc cagctggata gcgttgggtt ttgggatgtt tcggatcgga aaagtcactc
       61 acccagattt caccgccgtc gaacaaggca tagccgaaac ggttgcccga caaatcggcc
      121 agtccaacca ctttcgaaaa cttccccggc gcatactcgg ccggaacctc cgagagcaga
      181 tcgagtgttt ctgcgtcgaa cgccttgatg cctcccggcg tgtaattctg tgcgacgaca
      241 atgcggccgt cctgcgaaat cgatccgccg atcgagttgc cggcctgcat cacgcgcttg
      301 acgattcgcg cctcaagcag atcaatcttg gtcaggccac cgtcgcgacc gaagatgtag
      361 gcatagcgcc cgtcgcgcga ataaacggcc gaagcatgcg aaagatcgcc gagaccttca
      421 atgtgcgcat aaggcgcgcg tttgctggta tcgaccagta tcacgctgcc actcgcgcgc
      481 tcaatcacca gaccgagatc gccggtaccg cgcagttgcg gcgacgcaca ggccgaaaga
      541 aacaatgcaa gaaacagcga agacagcagc acgaaagaac gaaacaggga gaatctcatc
      601 atcaactcat acaaaggtag caacggcagg agcatgcacc atctattatc ggaatacttt
      661 ggtatacgtc aaagggtttg tttgattcct tgttgcttat gaggccgcag ccggctgcca
      721 gaagcttaca atagccgcct ctgctgtaaa acgacaccat gcccaacaat cgcgacgccc
      781 tggaactcct ggccccggcc aagaatgccg aattcggtat tgaagcgatc agtcacggcg
      841 ccgatgccgt gtatatcggc ggcccggctt ttggcgcgcg cgtaaatgcc ggcaactcgg
      901 ttgccgatat cgaacggctg gccagccacg cacaccgctt caatgcccgc gttctggtcg
      961 cgatcaatac cctgctcaag gacgacgaac tggaagaagc ccgacggctg atctgggagt
     1021 gctacgaagc cggcgccgac gcgctgatcg tccaggacat gggcctgctc gaactcgacc
     1081 tgccgccgat cgaactgcac gccagcacgc aaaccgatat ccgcacgccg gaaaaagccc
     1141 gctttctcga agacgtcggc ttttcgcaga tcgttctggc nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn ttcaagatcg aagggcgctt gaaggatctt tcctacgtca
     1321 agaacatcac cgcgcactac cgtcggcaac ttgacgacat catcgaggat attccctctt
     1381 atcggcgcag ctcatcgggt gtaactacac ttttctttac cccgcagacg gagaagacct
     1441 tcaaccgggg agcgaccgat tatttcctga atgatcgcaa atcggatatc ggcgcctttg
     1501 attcgccgaa atttgtcggc gagaaaatcg gcacggtcat cggaatcgga cgcgacagta
     1561 ttgacgtttg cagcgatgct ccgctgcaca acggcgacgg cattacctgc ttcgatgccg
     1621 atctcaagct cgtcggcctg cgcatcaacc gtgcgcagca agttgcttcg ggttaccgga
     1681 tcttcccaac tccaggcgaa ggtcgattcc cgaccgatct aaggattggc gagacgatct
     1741 atcgcaacca tgatcaggaa ttcgtgcgtc aactggagaa gaaatctgcc gagcgaagca
     1801 ttcgggtacg catgcaattc gaggaaacgg gtgatggctt cgccttgcat atcacggacg
     1861 atgaaggtgt cggtgcggtg gctcacgtgg cgcacgacaa gaaagccgca aacaaccctg
     1921 aattggcgat gaccagattg cgcgagaatc tgggcaaact gggcaacacc atttaccgca
     1981 gcgaaacgat cgatcttgcc atcgacacgg cgtggttcat tcccgattcc gtgctgaatg
     2041 cactgcgccg cgccgccgtc gaagcgctcg atgcggccag gaatgccgcc tacaagcagc
     2101 ctgaacgccg cgccgcgatg gtgcctcccg ctccctaccc ggatgatgcg ctcagctatc
     2161 tgggcaatgt gctcaacgcg aaggcgcgcg ccttttatga gaaacacggg gtcagcgtca
     2221 tcgcacctgc ctacgagtgc aacgccgaaa aaggcgaagt ctcgctgatg atcaccaagc
     2281 actgcctgcg ctacagcttc aatctctgcc ccaagcaggt caaaggcatc cgtccggacc
     2341 cgatgatcct gatgaacggc aacgagaagc tgaccctgcg ctttgactgc aagccctgcg
     2401 aaatgcatgt catcggcaag ctgaggaaaa accgaacgat ccagctggcg atcaggtagt
     2461 tcccaaactg ggttcagtaa tgcggcggga tttcatcacg caggctggaa ttttcatccg
     2521 gttcggcatt gtccttgatc tgttggtaaa gcagccgcat ctgctcctgc aacaaatcga
     2581 tctgctgctg ttgccgatac accgtgcgat tgagctcctc gagcagatcc tcgttgagac
     2641 tcgtctttat ttccagctcg gtcagtcggt ctttcatggc aagctctttc tggtcaagat
     2701 gaaatgcaca gtgaatggcg gacagcgaca aatcgctttc cggcgtaagg atcactgtgg
     2761 cctgattggc ccgattacga cgcgtcggac tataatccct ggaaattcct cggtcatcat
     2821 gtaaggaaca acggcatgtc actcgaaccc tgccgcgctt gtggacatca ggtcgatact
     2881 tcggcgctcg cgtgtcccgg ttgcggcgct accgatccgg caaaaaggat cagtcgtcag
     2941 cagcacaatg cgcgcgtctt tgtcattcaa ctgatcgtgt gtctcagcct tcttggattc
     3001 ggcggctggt atgtctggag taccctggtt cccgtgatca agcagtttat cgcgaaacca
     3061 caagccgaac agactcaggc cggccagcag taagcgatca gcccaaaaac cttgcacatt
     3121 tgcacaatgc tattggcctg ttgacagcgc cggcgtctca tttgtcgcac cgatcatcat
     3181 gcggcgtgtt tccggtgtct ccagactgat ccgaccaagc ttgccttcac ggtaatcctg
     3241 caccagtacg gccgaagcct tctcgaaatc gggaccgcca ccgcgcaaaa tcagggaacg
     3301 tttcttggcg acgccttcaa cgaccgccac cgcgtcgaca ccgtcaagta cgaaaccata
     3361 ccgtgacgaa agcaacgccg gatagcgtac cagcaagagc ccggcaagaa acaccgccac
     3421 ctcctcgtcg atcacggcat tacggccgat ggcgtggctc gcagcgagca tcaggccatc
     3481 gctcgggtat tcgattttcg gccacatcag acccggcgta tcggtgattg agtggcgaac
     3541 gctgatctga tgcgtctgct gcgacttggt cactgcgggc tcgtcaccaa ccttggcgat
     3601 cttgcgcttg accagcgcat tcatcaaagt cgatttgccc acattgggaa tacccatgat
     3661 catcatgcgc aagggtttga tgttgtcatt gcgatgcggt gcgaggcgct gacacagtgc
     3721 agggattcgc gccacctcgc cctgattctt gctcgacagc gcaacagctt tcacgttttc
     3781 ttgccggttg tagaagtcaa tccaggatcg ggtggcttcc gggtcggcaa gatccgcctt
     3841 gttcaatacc ttgagacagg gtcgctgacg gtgcaggcgg agttcgcgca ccagcggatt
     3901 ggtgctggct tcgggcagac gagcgtccag cacctcgatc accacgtcaa tcgacgccat
     3961 cgtttcggcc gccttcttgc gtgccgaggt catatgcccg ggataccact gaacagccat
     4021 gacgctggaa cctctgaaaa aggacgccat tatcccattg cttcgacgat ccacgtcaat
     4081 gcgcttaaaa tgggcgttta ttgcaaacgt gcatcatgaa gcccgtaata cccattcccg
     4141 agatcaaacc cgagcaatcc atcgaactgc tcaaggagct gcacatcctg acccgcgaag
     4201 gcaagctcaa tcaggacagt cgccgcaagc tcaagcaggt ttatcatctt taccacttca
     4261 tcgagcccct gctcgccgaa ttgctggctg gcaatgaagc catgacgctg gccgatcacg
     4321 gcgccggcaa gtcctatctg ggcttcattc tcttcgacct gtttttcaag aaccggactg
     4381 gcggccatat tttcggcatc gaaacacgcg aggaactggt cgacaaatcc cgcgaattgg
     4441 cgcaacgact gcaattcggg cgcatgagct tcctcaatct gagtattgag gactctatcc
     4501 gcgtagccac cctgccggag cgtattgaca tggtcaccgc gctacatgcc tgcgacacgg
     4561 cgaccgacga cgcaatccgt ttcgccctgt gcaagaaagc acggttcatc gtgctggtcc
     4621 cctgttgtca ggccgaggtt gcggccgcac tgcgccggaa caagaacgat tcgttttcaa
     4681 aaacaccgct ctcggaactc tggcgccacc cgatccatac ccgtgaattt ggcagtcaga
     4741 tcaccaacgt gctgcgctgt ttacagctcg aagcttgcgg ctatcagata caggtaaccg
     4801 aactggtcgg ctgggagcat tcgattaaaa atgaactgat catcgccaga aagaccggcc
     4861 aagtgcgcaa gaatgccgcc gatcggctgg aagcgatatt gcacgaactg aacctcgacg
     4921 agttgcggga acggttcatg ttctccgggt aaatggacag aattttcttc aagcgcgcac
     4981 aatcgcacac taggactgac atggataact ggatcacccg aatcgccgca gccctctgcg
     5041 ccgcaggcag cactggtttg ttctggatgt ttggcgtgtt catcgccgtg ccttggcgcg
     5101 aaggtcgcat gctggcactc accaagaccg aattgcaagt cgtcggcatt ccgctagtga
     5161 ttggatttgc cgtcgcctgg ggggcgctgc atatcttcgc gatatcggat cgcgctgcaa
     5221 acccgaaggt ttacgcgacg atccgctggg tggtgatcct catcgctatc gccgccgtga
     5281 tcggcggcaa ggcatggacc gacgcgagaa ttgcctgagt gcaggcaata acaaccacaa
     5341 gcaagaaacg ccttaggccg ctgctcgggc cgttgctcag gccgtagtat tgacccgtgt
     5401 ccccaccgtc tgcccgttgg cgttgaccgt cggtctactg ccttccacat cggactgcgc
     5461 acgatcggct gccctgctct cttcaaccga ctggttgctt tgcgtttcct gcgttccttg
     5521 ggcctgctgg gactgttgcg attgctgcga ctgctgcgtc tgctcatcct gctctggcgg
     5581 acgcgcgcga acctgtgctt gcaaggccga ggccgaggct gtgttgatgc tggatgatac
     5641 tgaacctgct tccatggctg aatcctcctg ctccgggcct gaacgaaccc agctatcact
     5701 atagaccatt tcgcgcgctt ttcccttgcg gttgccaaca tcgacc
//
LOCUS       DAORPM010000157         5731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3876_length_5731_cov_14.237491, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000157 DAORPM010000000
VERSION     DAORPM010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3876_length_5731_cov_14.237491"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLD53_08290"
     CDS             complement(<1..277)
                     /locus_tag="PLD53_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRC60952.1"
                     /translation="MNNFLFYLAAFAPLLGALIIFHEYGHYLVARLAGVKVLRFSIGF
                     GRAIWSKRIGRDGTEWCLGAFPLGGYVKMLDEREGEVDPRELHRSFNR"
     gene            complement(274..1464)
                     /gene="ispC"
                     /locus_tag="PLD53_08295"
     CDS             complement(274..1464)
                     /gene="ispC"
                     /locus_tag="PLD53_08295"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRC60953.1"
                     /translation="MTLQKLTILGSTGSIGVNTLDVVRRHPQRYRVIALCAHNQIDRL
                     FEQCLEFRPRFAVVRDAELAAELQRRLRLAASSIHVESGPEALVRMAELPEVDSVMAA
                     IVGSAGLQPTLAAAVAGKKILLANKEALVMAGPVFMRAVRESNSTLLPIDSEHNAIFQ
                     SLPSDYSGSLLKSGVRSILLTASGGPFRNTPVADLEHVSPEQACAHPNWVMGRKISVD
                     SATMMNKGLEMIEAHWLFNVPAEQIEVVIHPQSVIHSLVQYVDGSVIAELGNPDMRTP
                     IAHGLAYPERINAGVEPLDLCKIASLHFERPDFERFPCLDLAFRALRAGHSAPTTLNA
                     ANELAVQAFLDGRIGFTAISRIIATVMDMSPVTELAALSDVIDADSAARKLAEQIIGE
                     MVSV"
     gene            complement(1474..2310)
                     /locus_tag="PLD53_08300"
     CDS             complement(1474..2310)
                     /locus_tag="PLD53_08300"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRC60954.1"
                     /translation="MLKTRILTAIFLLSAFFLALFYLPPLGWSMFATLIAAIAAWEWG
                     GLMGFGNNLRIMLGGAFIFVCAALVALEPAALGLEDGFSEAAWALGRWFYLPAAAFWL
                     LGVPVWLRRRLPLPKSLLGLLTGCVLILPTWLALIQLRQAGATTLVVIMATVWVADTG
                     AYFFGRTFGSHKLAPSISPGKTWEGAVGGGLTVIVYGFVFSSKLPAVLSDSFVLLLSA
                     LILLTVISIMGDLFESLLKRQAGLKDSSNVLPGHGGVLDRIDSLTSTLPLVALVWLTV
                     VY"
     gene            complement(2303..3133)
                     /gene="uppS"
                     /locus_tag="PLD53_08305"
     CDS             complement(2303..3133)
                     /gene="uppS"
                     /locus_tag="PLD53_08305"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRC60955.1"
                     /translation="MAIFTSSTRDIPTTAAVPRHVAIIMDGNGRWAKKRCLPRFAGHR
                     QGVETVRDTIKYCLEAGIEYLTLFAFSSENWRRPEEEVSLLMQLFMRALQQEVSKLER
                     NGVRLKVVGDLSPFDRDLQSLILASEQQTAVNDKLTLTIAANYGGRWDILQAVNRMVL
                     AHPEKKGVWSESDLAPYLAMNYAPEPDLFIRTGGEERVSNFLLWQLAYAEFYFTDTLW
                     PEFDSTAFAAAISSYQQRERRFGRTSEQLIRGPLSVVNADHSPENAGLVRPANLKINA
                     "
     gene            complement(3157..3717)
                     /gene="frr"
                     /locus_tag="PLD53_08310"
     CDS             complement(3157..3717)
                     /gene="frr"
                     /locus_tag="PLD53_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRC60956.1"
                     /translation="MSIAEVKKTTEHKMQKSLEALRVDLAKVRTGRAHIGLLDHVQVD
                     YYDSMVPVNTVANITLIDSRTLGVQVWEKTLAPKVEKAIRDSDLGLNPASQGELIRVP
                     MPALTEERRRDLIKVVKHECEGARVAMRNLRRDANATLKDLLKQKECSEDDERRAQDD
                     IQKLTDRHVAEVDKQFAQKETELMAV"
     gene            complement(3729..4454)
                     /gene="pyrH"
                     /locus_tag="PLD53_08315"
     CDS             complement(3729..4454)
                     /gene="pyrH"
                     /locus_tag="PLD53_08315"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRC60957.1"
                     /translation="MTETAIPKFKRILLKLSGEALMGSDAYGINRQTISEVVSEVKSV
                     VDLGVQVGVVIGGGNIFRGVAPAATGMDRAQADYMGMLATVMNGLALQDAMRVAGMVA
                     RVQSALNIEQVVEPYIRGRAIRYLEQGRTVIFAGGTGNPFFTTDTAAALRGAEIGAEI
                     VLKATKVDGIYSADPKKDPLATRFDRISFNEAISMNLAVMDSTAFALCRDQKLPINVF
                     SIFKPGALKRVVLGENEGTLVYS"
     gene            complement(4475..5368)
                     /gene="tsf"
                     /locus_tag="PLD53_08320"
     CDS             complement(4475..5368)
                     /gene="tsf"
                     /locus_tag="PLD53_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRC60958.1"
                     /translation="MAAITASMVAELRAKTDAPMMECKKALTEANGDLGKAEEILRVK
                     LGNKATKAATRITAEGAVTISISADGKVGSIIEINCETDFVAKNDEFLQLLTGCADLV
                     VDKNPADVAALSLLPMKDGSVESTRSALVGKVGENMSIRRFARVEAKGKLASYIHGGS
                     KIGVLLDLQGGDEQLGKDLAMHIAASKPKALDSKGVSVELLDTERRIAIEKARADNKP
                     EAMLEKIAEGTVQKYLKEVTLLAQVFVKAEDGKQTIEQLLKIKNASVAGFTLYVVGEG
                     IEKRSNDFAAEVAAQAAAAQK"
     gene            complement(5487..>5731)
                     /locus_tag="PLD53_08325"
     CDS             complement(5487..>5731)
                     /locus_tag="PLD53_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRC60959.1"
                     /translation="HKIAITEANKLGIPVIAVVDTNHTPEGIDYVIPGNDDSSSAIRL
                     YARGVADSILEGRSQFVEEIVAAAESGDEFFEEAEQ"
BASE COUNT         1375 a         1669 c         1438 g         1249 t
ORIGIN      
        1 gccggttgaa gctgcgatgc aattctcgtg gatcgacctc accctcgcgt tcatcgagca
       61 ttttgacgta gccgccgagg ggaaacgctc caaggcacca ttcagtgccg tcgcgaccaa
      121 ttcgttttga ccatatcgcc cgaccgaaac cgatagagaa tcgcaaaacc ttgacaccgg
      181 ctaggcgtgc cacgaggtag tgtccatatt cgtggaagat aatcagcgcc ccaagaagcg
      241 gagcaaacgc agccaaatag aaaaggaagt tgttcatacc gaaaccatct cgccgatgat
      301 ttgctcggcg agcttgcgtg ctgcactatc agcgtcaatc acatcgctga gcgcggccag
      361 ctctgtaacc ggcgacatat ccataactgt tgcaataatc ctcgatatcg ctgtgaatcc
      421 gatccgccca tcgagaaacg cctgaaccgc aagctcattg gcggcattca gggtcgtcgg
      481 cgcgctatga ccggcgcgca gggcgcggaa agccagatca agacagggga aacgctcgaa
      541 gtcagggcgc tcaaaatgca atgaagcgat tttgcacaga tcaagcggct cgacgccggc
      601 attgattcgc tccggatagg ccaaaccgtg ggctatcggc gtgcgcatgt ccggattgcc
      661 cagttcggcg atcaccgagc catccacgta ttgcaccaac gagtgaatga cgctttgtgg
      721 gtgaatgaca acctcgatct gttcggcggg gacgttgaac agccagtgag cctcgatcat
      781 ctcgagcccc ttgttcatca ttgtcgccga atctaccgat atctttcgtc ccataaccca
      841 gttcggatga gcacaggcct gctctgggct gacgtgttcg aggtcggcga caggggtatt
      901 ccggaacgga ccgccggaag cagtcagcaa tatgctacgc acaccgcttt ttaataaact
      961 acctgaataa tcactaggaa gtgattgaaa tatagcgtta tgttcgctat ctataggcaa
     1021 cagagttgaa ttgctttcac gcacggcccg catgaagacg ggtccagcca tgaccaaggc
     1081 ttccttgttt gccaacagga ttttcttgcc ggccactgcg gcggccagtg ttggttgcag
     1141 ccctgcagaa ccgacaatgg cggccataac cgaatcgact tccggtaatt ccgccattcg
     1201 gaccagggct tcaggcccgg actccacatg aattgagcta gcagccaacc ttagtcggcg
     1261 ttgcaactcc gcagccagtt cagcatcccg taccacggcg aaacgcggcc gaaactccag
     1321 acattgttca aacaagcggt cgatctggtt atgggcacaa agggcaatta cccgataacg
     1381 ttgcggatga cgtcgaacca catccagtgt attgacacct atggatcccg tagatccgag
     1441 aatcgtgagt ttctgcaaag tcatcacaaa ctgtcaatac acgacagtca gccagaccaa
     1501 ggccaccagc ggcagtgtcg aggtaagact atcaatccga tcaagcacgc cgccgtgccc
     1561 aggcagaaca ttgctgctgt ctttcagacc ggcctgacgc ttgagcagcg attcaaagag
     1621 gtcgcccatg atactgatca ccgtcagaag aataagcgca gataacaaca aaacaaagct
     1681 gtcagaaagt actgccggca actttgagga gaagacaaag ccataaacga tcactgtcaa
     1741 gccaccgccc accgcacctt cccaagtctt gcctggactg atgcttggcg ccagtttgtg
     1801 acttccgaag gtgcgcccga aaaaataggc gccagtatcc gctacccaga cagtagccat
     1861 tatcacgact agcgttgtcg caccagcctg cctcagttgt atcaaagcca gccaggtcgg
     1921 caggatcagc acgcagccag tcagcaaacc gagtagagat ttcggcagcg gtaagcggcg
     1981 tctcagccac acgggcacgc cgagcaacca gaacgccgcc gccggaagat aaaaccagcg
     2041 ccccaaggcc caagccgctt ctgagaatcc atcctccagc cccagtgctg cgggttccag
     2101 agcaacaagt gccgcacaaa cgaagataaa cgccccgccc aacatgatac gcaagttatt
     2161 gccgaaaccc atcagcccgc cccactccca ggcggcaata gcggcaataa gggtagcaaa
     2221 catgctccag ccgagtggag gcaaataaaa cagtgccagg aaaaaggccg ataaaaggaa
     2281 gatcgcggta agtatccgcg ttttaagcat ttatcttcaa gtttgccggg cggaccaaac
     2341 ctgcgttctc cggcgaatga tcggcgttca caacagacaa agggccacgg atcagttgtt
     2401 cgctcgttcg cccaaaacga cgttcacgct gctgataaga ggaaatcgcc gcagcgaagg
     2461 ccgtcgagtc aaattcgggc cagagggtgt cggtaaagta aaattcagcg taggcaagtt
     2521 gccacagcag aaaattactg acccgttctt ccccgcccgt acgaatgaaa aggtcgggct
     2581 ccggtgcgta gttcatggcg agataaggcg caaggtcaga ttcactccag acccccttct
     2641 tttccggatg cgccagaacc atgcgattaa ctgcctgcaa gatgtcccat cgcccgccgt
     2701 agttggccgc gatggtcaga gtcaacttgt cattgacggc tgtctgctgt tcggaagcga
     2761 gaatcagcga ctgcaaatcc cgatcaaaag gtgaaagatc accaacgact ttcaggcgaa
     2821 caccattgcg ttccagcttg gaaacctcct gctgcaacgc acgcatgaaa agctgcatga
     2881 gcaggctaac ttcctcttcg gggcggcgcc agttttctga actgaaagca aagagggtca
     2941 ggtactcgat tccggcttcg agacaatact tgatcgtatc gcgaaccgtc tcaacacctt
     3001 gacgatgccc ggcaaagcgt ggcagacagc gctttttggc ccagcgtcca ttgccatcca
     3061 taattatggc aacgtgacgc ggcactgcgg cagtggtcgg aatatcccga gttgaactgg
     3121 taaagatcgc catttagagt gcctttgcga cggacttcaa acggccatca actctgtttc
     3181 tttttgggca aactgtttgt cgacctctgc aacatgccta tcagtcaatt tttggatatc
     3241 atcctgtgcc cggcgttcat catcctccga gcattctttc tgcttgagaa gatctttaag
     3301 ggtggcattc gcgtcccgtc gcaggttacg catggcaaca cgcgcgcctt cacactcgtg
     3361 cttgaccacc ttgatcaggt cgcgacgtcg ttcttcagtc agcgcaggca tcggaacgcg
     3421 tatgagctca ccctgtgacg ccggattaag tccaagatcg gaatcgcgaa tggccttttc
     3481 gacctttggc gccaaagttt tctcccagac ctgaacaccc agtgttcgag agtcaatcaa
     3541 ggtaatgttg gcaacggtat tcaccggcac catcgagtcg taatagtcga cttggacatg
     3601 atccagcagc ccgatatgag cacggcccgt acgaaccttc gccaaatcga cccgtagcgc
     3661 ctcaagcgat ttctgcatct tgtgttcagt ggtcttcttc acctcggcaa tcgacatttc
     3721 ttcctccctc agctatacac cagcgttcct tcgttctcgc cgagcaccac ccgcttcagc
     3781 gcgcccggtt tgaagatcga gaagacattg atcggcagtt tctggtcacg acacagcgca
     3841 aaagctgtcg aatccatcac ggccaggttc atggaaatcg cttcgttgaa actgatccga
     3901 tcaaatcttg tcgccagggg atccttcttc ggatcggcag aataaattcc atcaaccttg
     3961 gttgctttga gtacgatctc tgccccaatt tccgcaccgc gaagggcagc tgccgtatcc
     4021 gtcgtgaaaa aaggatttcc cgtaccgcca gcaaagatca cggttctacc ctgctccaga
     4081 taacggattg cccgaccacg aatatagggc tcgacgacct gttcgatatt cagtgccgac
     4141 tggacgcggg caaccattcc agcgacgcgc atcgcatcct gcagtgccag accgttcatc
     4201 accgtggcca gcattcccat gtagtcagcc tgcgcacgat ccattcctgt agcagcggga
     4261 gcaacgcccc gaaagatatt tccgccgccg atcacaacgc ctacctgaac ccccaaatcg
     4321 acaacgctct tgacctccga aacgacttcg gaaattgtct ggcggttgat gccgtaagca
     4381 tcagaaccca tcagtgcttc acctgagagt ttgagcagta ttcgtttgaa tttggggatc
     4441 gcagtttcgg tcatggcagt cggaattccc tggtttactt ctgtgcagcc gccgcttgag
     4501 cagcaacttc ggcagcaaag tcgttcgacc gcttctcaat gccttcgccg accacgtaca
     4561 aggtaaaccc ggcaaccgaa gcattcttaa tcttgagcaa ctgctcaatg gtctgcttgc
     4621 cgtcctcagc cttgacgaac acctgagcca gcagagtcac ttccttcagg tatttctgaa
     4681 ccgtaccctc ggcgatcttc tcgagcattg cttccggttt attgtcggcg cgcgcctttt
     4741 cgatagcaat gcggcgctcg gtatcgagga gttcaactga aacgcccttg ctgtccaatg
     4801 ctttcggctt ggaagcagcg atatgcatcg ccagatcctt gcccagttgc tcatctccac
     4861 cctgcaggtc cagcagcacg ccgattttgg agccgccgtg gatgtatgaa gcaagcttgc
     4921 ccttggcctc tacgcgggcg aaacggcgaa tcgacatgtt ttcgccgacc ttgcctacga
     4981 gcgccgatcg ggtcgattcg acactaccgt ctttcatcgg cagcagcgac agggcggcga
     5041 cgtcagcagg attcttgtcg actaccagat ctgcacaacc cgtgagcagt tgcaagaact
     5101 cgtcattctt tgcaacaaag tctgtttcgc aattgatttc gatgatcgaa cccactttgc
     5161 catcggctga aatactgatg gtaaccgctc cttcagcggt gattcgagta gcggccttgg
     5221 tcgctttgtt gcccaatttc acacgtagaa tttcttcggc cttgcccaga tcgccattgg
     5281 cttcggtcag cgccttcttg cattccatca tcggtgcgtc ggtctttgca cgcagttctg
     5341 ccaccatgct tgcggtaata gccgccattt ttctctccac ttgcaggcat ggccggacaa
     5401 ccggccgcgc ctgattaaaa cagttcaaac gaattgtttc cggtcacact tgccattgca
     5461 ggcaagtgga gcaaaacgat cgaggattac tgctctgctt cctcaaaaaa ttcatcgccg
     5521 gattcagcag cggcaacaat ttcttcaacg aactggctgc gaccttcgag aattgagtcg
     5581 gctacacccc gcgcatacaa acggatcgcg cttgaagagt cgtcgttgcc cggaatgacg
     5641 taatcaatgc cctccggtgt atggttggta tcgacaacgg cgataaccgg aataccaagc
     5701 ttgttcgctt cggtaatggc aatcttgtgg t
//
LOCUS       DAORPM010000158         5716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3885_length_5716_cov_14.193075, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000158 DAORPM010000000
VERSION     DAORPM010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_5716_cov_14.193075"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..478)
                     /locus_tag="PLD53_08330"
     CDS             complement(74..478)
                     /locus_tag="PLD53_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HRC60960.1"
                     /translation="MLPKMLTMLPAFLAYFAVGVVLITVFFMVYVNVTPYDEIALIRQ
                     GNIAAAIGLSGALFGFALPVANVIAHSDTLIDLAAWGAIAGVIQILAYLVARYTVPHL
                     AEDIPAGKVAPAIFVATLSLTVGLTNAACMTY"
     gene            complement(531..1262)
                     /locus_tag="PLD53_08335"
     CDS             complement(531..1262)
                     /locus_tag="PLD53_08335"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRC60961.1"
                     /translation="MKLIAIDAGNTRIKWGVHDGAIWIARGALPTSEAASLSEVSAGW
                     PGAARIVACNVAGQVVEDVIGTALGTRFPAPLWLRSSAGICGVRNAYDQPGSLGADRW
                     AALIGARGLSSTDCLVVGAGTATTVDWLDASGVFRGGLILPGIDLMRASLARDTAQLP
                     LAEGKFSTTPRSTVDAIASGCLHAQIGAIERMFSELPASPETICLLTGGAAHRIAPHL
                     LIPFKLTENLVLDGLIRFAVMHPSN"
     gene            complement(1259..2071)
                     /locus_tag="PLD53_08340"
     CDS             complement(1259..2071)
                     /locus_tag="PLD53_08340"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRC60962.1"
                     /translation="MIDSSSSPMIDPVSVQSMLGDARGRFDIDSLQECDSTSTLLLKR
                     AAEGAPAGSVIVTDRQTAGRGSRGRGWLASPESSLTFSVLWRFDGGVGQLAGLSLAVG
                     VAVVHALEAFGANGIMLKWPNDILYDNAKLGGILVELQSAPDSALAVVGIGLNLSMPE
                     SIGEAADFALPAAALDDIILPLPERHALFAQLLVELAHVFDRFSYGGFAVLRDQWQAL
                     NAWQNHPVRLLRNGLLEKEGVCRGADLDGALLVQTSAGVERCLSGDLSLRAR"
     gene            complement(2131..3555)
                     /gene="ntrC"
                     /locus_tag="PLD53_08345"
     CDS             complement(2131..3555)
                     /gene="ntrC"
                     /locus_tag="PLD53_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HRC60963.1"
                     /translation="MKPVWIVDDDKSIRWVLEKTLSREQVPFKSFASASEALSQLDLG
                     REPPQVLLSDIRMPGESGLDLLKQVKERFPSLPVIIMTAYSDLDSAVAAFQGGAFEYL
                     PKPFDVDQALELIRRAIDESLHQVGASDEVGLVPEILGQAPAMQEVFRAIGRLSQSNA
                     TVMITGESGSGKELVARAVHRHSLRSDKPFIAINTAAIPKELLESELFGHERGAFTGA
                     AVQRRGRFEQAEGGTLFLDEIGDMPSELQTRLLRVLSDGNYYRVGGHSPIKANVRIIA
                     ATHQNLENRVKEGLFREDLFHRLNVIRVRLPSLRERREDIPILARHFMHKSAQELGVE
                     AKRFSEAALKHLASLDFPGNVRQLENICHWLTVMAPGQQVDLADLPAELRDTALQSAA
                     ADWESALAQEVNRLLVDSGGKVHEILTNSFERVLITRALAHTGGRRIEAALALGIGRN
                     TITRKIQELGLDDGRNSDPLRATG"
     gene            complement(3620..>3766)
                     /locus_tag="PLD53_08350"
     CDS             complement(3620..>3766)
                     /locus_tag="PLD53_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRC60964.1"
                     /translation="PLVSGRQGGSGLGLTLAQSFIQQHQGTVECETRPGRTCFTIRLP
                     LITE"
     assembly_gap    3767..3866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3867..4516)
                     /locus_tag="PLD53_08355"
     CDS             complement(<3867..4516)
                     /locus_tag="PLD53_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRC60965.1"
                     /translation="MSDTTQNPFGSLDLLSSSVILLDANFNILYMNSAAENLLAISSK
                     TFSGCRFDSVVECPSALQDVFDNAFSSDWSFTGQSIKLNRSDGVALHLNCTVNPTDTM
                     DARLLVELWPIDQQLRATREERLLEQTIASRELIRNLAHEIKNPLGGIRGAAQLLEHE
                     LNNPGLREYTQVIINEADRLQDLMKRLLSPHRPMQPGPVNIHEILERVRSLLSAEFP"
     gene            complement(4552..5043)
                     /locus_tag="PLD53_08360"
     CDS             complement(4552..5043)
                     /locus_tag="PLD53_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRC60966.1"
                     /translation="MNSQRLYFPLALLFLVAIPAQAAVMYQCVDESGHKSFSNMKSAT
                     KGAKCTAMDLGPASAPAAKGATKTPTPTNFPKVEENTQRARDTDRLRILEGELDAEKR
                     NLAQAQKDLAEQEATTLPSERMQGGGISGGKVAERVQPFKDKVALHERNIEAIQKEIS
                     KLR"
     gene            complement(5206..>5716)
                     /gene="glnA"
                     /locus_tag="PLD53_08365"
     CDS             complement(5206..>5716)
                     /gene="glnA"
                     /locus_tag="PLD53_08365"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289488.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRC60967.1"
                     /translation="GIIKHAKALNAITNPGTNSYKRLVPHFEAPVKLAYSAKNRSASI
                     RIPHVASDKARRIETRFPDPIANPYLCFTALLMAGLDGIQNKLHPGDAADKNLYDLPP
                     EEDAKIPTVCASLEQALDALDKDRDFLTRGGVFSNEWIDAYLELKMDEVNKVRMTTHP
                     VEFDLYYSC"
BASE COUNT         1185 a         1621 c         1659 g         1151 t
ORIGIN      
        1 atgacgtcaa taaactgttt cttgatgaaa tccatgagtg ccatgaggtt ctccctgaac
       61 gaaagcagcc tgattagtag gtcatgcacg ccgcgttggt cagcccaacg gtcagcgaaa
      121 gtgtggcaac aaagatcgcc ggggctactt tgccagcggg aatatcttca gccagatgcg
      181 gcacggtata gcgtgccacc aggtacgcca gtatctggat cacgccggca atagcgcccc
      241 aggcagcgag gtcgatcagc gtgtcgctgt gggcaatgac attggctacg gggagggcga
      301 agccgaacag ggcgcccgac aggccaattg cggccgcgat gttgccctgc cggatcaagg
      361 cgatttcgtc gtacggcgtg acgttcacat agaccataaa aaatacggta atcaggacga
      421 cgccaaccgc gaaataggcg agaaaggcgg gcaacatggt caacattttg ggcaacatag
      481 aactctccag tatgcgaatg ctgcgtcgcc gttccagacc ggcaaatact tcagttcgac
      541 gggtgcatga cagcaaaacg aatcagtccg tcaagaacca gattctctgt caatttaaac
      601 gggataagca agtgcggtgc gatgcgatgc gcggcgccgc ccgtcagcag acagattgtt
      661 tcgggggagg caggcaattc cgagaacatt cgctcgatgg cgccaatctg ggcgtgcaaa
      721 cagccactgg cgatggcatc cacggtactc cgtggcgtcg tgctgaattt gccttccgcc
      781 aatggcagtt gtgcggtgtc acgtgccaaa gaggcccgca tcagatcgat acccggcagg
      841 atcaatccgc cacggaatac tccgcttgcg tcgagccagt cgacagtggt cgccgttccc
      901 gcgcctacga ccaggcagtc cgttgagcta agcccgcgag cgccgatcaa cgccgcccag
      961 cggtcggcgc ccaggctccc gggctgatca taggcattgc gcactccgca aattccggcg
     1021 cttgaacgca accatagcgg ggcaggaaag cgtgtgccca atgccgttcc gattacgtcc
     1081 tcaaccactt ggccggccac gttacaggcg acgatgcgtg cagcgccagg ccacccagcg
     1141 gagacttcgg acaagctcgc ggcttcgctg gtgggcaggg cgcctcgggc aatccagatc
     1201 gcgccgtcat ggacgcccca cttgatgcgc gtattgccgg cgtcgatggc gatgagcttc
     1261 atcgggcgcg caaggaaaga tcgccggaca ggcagcgctc gacgccggcc gaagtctgca
     1321 ccagcagggc gccgtcaagg tcggcgccgc ggcagacgcc ttccttttcg agaaggccgt
     1381 tgcgcagcag acgtaccgga tggttctgcc aggcgttcag cgcctgccac tgatcacgca
     1441 agacggcaaa accgccgtac gagaagcggt cgaatacatg cgccagttcg acgagaagct
     1501 gcgcgaacaa ggcgtgtctt tccggcaatg gcaagatgat atcgtccagc gcggcggcag
     1561 gaagtgcgaa gtctgcagcc tcgccgatgc tttcgggcat gctcaggttg aggccgattc
     1621 cgacgacggc cagcgcgctg tccggcgcgc tctgcaattc gacgagaatg ccgccgagtt
     1681 ttgcgttgtc atacaggatg tcgttcggcc acttgagcat gatgccgttg gcgccgaagg
     1741 cttcaagggc gtgaaccacg gcgacgccca ccgccagcga aaggccggcc agttgcccga
     1801 caccaccgtc gaaacgccaa agcacagaaa aagtcagact cgattcaggc gatgccagcc
     1861 agccacgacc gcggctgccg cgtccggcgg tctgccggtc tgtgacgatg acgcttccgg
     1921 ccggtgcgcc ttcagcggca cgcttgagca gcaatgtgct ggtcgaatcg cactcctgga
     1981 gggaatcgat atcgaatcgg ccgcgcgcat ctccgagcat ggattgcacg ctgaccgggt
     2041 cgatcatcgg tgacgaggaa gaatcaatca ctgcagtggc atcagaattc aatgccgcta
     2101 ttatcgtcaa taaccgcttc gactaccgaa ctatcccgtc gcacgcagcg gatcgctatt
     2161 gcgtccgtcg tcaagtccca gctcctggat cttgcgcgtg atggtgttgc gaccgatacc
     2221 cagtgccagc gctgcttcga tccgccgccc tcccgtgtgt gccagcgcac gggtaatgag
     2281 aacgcgctcg aagctgttgg tcagtatttc gtgaaccttg ccgccactat ccaccagaag
     2341 acgattgacc tcctgcgcca acgcactctc ccagtcggca gcggcggact gcagcgcagt
     2401 atcgcgcagc tccgccggaa gatcggcgag gtcaacctgc tgacctggcg ccatgacagt
     2461 aagccagtga cagatgtttt ccaattgacg aacgttgcca gggaaatcaa gcgacgcgag
     2521 atgtttgagc gctgcctcgg aaaaacgctt ggcctccacg cccagctcct gggcgctttt
     2581 gtgcatgaaa tgacgggcca ggatcggaat gtcctcgcgc cgttctcgca agctcggcaa
     2641 acgcacgcgg atcacattca ggcggtgaaa cagatcctcg cgaaagaggc cttccttgac
     2701 gcgattttcc agattctggt gtgttgcggc gatgatgcgg acgttggcct tgatcgggga
     2761 atgcccgcca acgcggtagt agtttccatc ggagagcacg cgcagcaagc gtgtctgcag
     2821 ttcagagggc atgtcgccga tttcgtcgag aaaaagcgtg ccgccttcgg cttgttcgaa
     2881 gcgtccgcgc cgctgcaccg cggctccggt aaaggcgcct cgctcatggc cgaatagctc
     2941 ggactcaagc agctccttgg gaatggctgc cgtgtttatg gcgatgaatg gcttgtccga
     3001 tcgcaaactg tggcgatgta cggcacgggc gaccagttcc tttcccgaac ccgactcgcc
     3061 ggtgatcatg accgttgcgt tcgactgact caggcggcca atggcgcgaa agacttcctg
     3121 catggcgggg gcctgcccga ggatctcagg aaccagacca acctcgtcgg acgcaccaac
     3181 ctggtgcaag ctctcgtcaa tggcgcgacg gatcaactcc agcgcctggt cgacatcaaa
     3241 cggtttgggc aaatactcga atgcgccacc ctggaaagct gcaacggcac tgtccagatc
     3301 cgagtaggca gtcatgatga tgaccggcag agaagggaag cgctctttga cctgctttaa
     3361 cagatccagc ccggattcgc ccggcatgcg gatatccgac aacaacacct gcggaggttc
     3421 cctgcccaga tcaagctgcg acagggcttc gctggccgaa gcaaaactct tgaatggcac
     3481 ctgttcgcgt gacagcgtct tttcgagaac ccagcgtatt gatttgtcgt cgtcgacgat
     3541 ccacacaggc ttcattgtat ttcctgacat tacgaataag tatggttagc ctttagcaaa
     3601 tagcttgcca ttttggcgat tactccgtaa tcaggggcaa tcggatcgta aaacaggtac
     3661 gaccagggcg tgtttcgcat tcgaccgtcc cctggtgctg ctggatgaag ctctgagcca
     3721 gggtcaagcc aaggccgcta cccccctgac gacctgacac cagcggnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnggga actcggcaga cagcaggctg cgcacacgtt
     3901 caagtatttc gtggatattc accgggcctg gctgcatcgg tcgatgcggc gaaagcaggc
     3961 gcttcatcaa atcctgcagg cggtccgctt cattgatgat gacctgcgtg tactcgcgca
     4021 atcccgggtt gttgagttca tgctccagca attgcgccgc cccacgaatg ccgcccagcg
     4081 ggttcttgat ttcatgcgcc agattgcgga tcagctcacg gctggcaatc gtctgttcga
     4141 gcaggcgctc ttcacgcgtc gcccgcaact gctggtcgat tggccagagc tcgacaagta
     4201 gccgggcatc catcgtgtcg gtcgggttca ccgtacagtt cagatgtagg gcgacaccgt
     4261 cactccggtt caacttgatg ctttgtccgg taaagctcca gtcactggaa aacgcattgt
     4321 cgaacacgtc ctggagcgcg ctggggcatt ccacaacact gtcgaaacga catccggaaa
     4381 aggtcttgct gctgatcgcc agcaggttct ctgcggcgga gttcatgtaa agaatgttga
     4441 aattcgcgtc gagcagaatg acagacgatg agagcaggtc cagactgcca aacggatttt
     4501 gcgtcgtatc agacatgcac gaatgagagc gatgggcgca ccatgtccgg atcagcgcag
     4561 ctttgaaatt tctttctgga tggcctcgat gtttcgctcg tgcagcgcca ctttgtcctt
     4621 gaaaggttgc acacgctcgg caaccttgcc tccgctgatg ccgccgccct gcatgcgttc
     4681 acttggcaat gtggtggctt cctgctcggc aagatctttc tgggcttgcg ctagattcct
     4741 cttttcagcg tccagttcgc cttcaagaat acgtagacga tctgtgtcgc gagccctctg
     4801 cgtattttct tccaccttcg gaaagttcgt cggcgtcggc gtcttcgtag cgccctttgc
     4861 tgccggtgcg gaggcggggc ccagatccat cgcggtgcac ttggccccct tggtggccga
     4921 cttcatgttt gaaaacgatt tgtgtccgct ttcatcgacg cactgataca tcacggctgc
     4981 ctgtgccgga attgcgacca ggaaaagtag tgctaacggg aaatacagcc tctgggaatt
     5041 catcacgctc ctattcattc gtggcagtaa gtgaccaaag atcaaacacc ggtttcagtg
     5101 tattggatga gccaatgaaa tacaaacgca ttaagctcga gtacggtgga caaaaaaagg
     5161 acgggcaggc ccgtccttta ttattgcaac cctgctaacg ttgcatcagc agctgtagta
     5221 caggtcgaac tcaactgggt gggtcgtcat gcgtaccttg ttgacttcat ccatcttgag
     5281 ttcgaggtag gcgtcgatcc actcgttgga gaaaacgccg ccacgggtca ggaaatcgcg
     5341 atccttgtcc agtgcgtcga gcgcctgttc gaggctggcg caaacggtcg gaatctttgc
     5401 atcttcttcc ggcggcaaat cgtaaaggtt cttgtcagcg gcatcaccag ggtgaagctt
     5461 gttctggatg ccgtccagcc ctgccatcag aagtgcagtg aagcacaggt aggggttggc
     5521 gatcggatcc gggaatcgtg tttcgatgcg gcgagctttg tccgaagcaa cgtgtggaat
     5581 gcggattgaa gccgagcggt tcttggccga ataggccagt ttgaccggcg cttcaaagtg
     5641 cggaaccagg cgcttgtacg agttggtgcc ggggttggtg atggcattca gcgccttggc
     5701 gtgcttgatg atgccg
//
LOCUS       DAORPM010000159         5694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3910_length_5694_cov_10.771413, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000159 DAORPM010000000
VERSION     DAORPM010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3910_length_5694_cov_10.771413"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(439..1248)
                     /locus_tag="PLD53_08370"
     CDS             complement(439..1248)
                     /locus_tag="PLD53_08370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRC60968.1"
                     /translation="MHPALNIIIKAARRASQIINRSANELEHLKVTTKRQSDFVTEVD
                     KAAEAAIIEVLSEAYPEYGILAEESGQTAGRGAGSDYQWIIDPLDGTTNYIHGFPQYA
                     ISIALTHMGQVTQSVVYDTMRNEMFTASKGAGTFLNDRRVRVSKCLKLEDALIGTGFP
                     YHSFEHIDAYLGTLKELMQKSSGIRRPGAASLDLAYVACGRLDGFWEFGLAPWDIAGG
                     ALLVSEAGGLIGDLSGNENYLETGNVIAGTPKVFAQLLQVVDSHRTARLGA"
     gene            1333..2133
                     /locus_tag="PLD53_08375"
     CDS             1333..2133
                     /locus_tag="PLD53_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRC60969.1"
                     /translation="MNPALASLGARSPHRLVTPLDRVHVVLSHPSHPGNIGAAARAMK
                     TMGLSHLTLVNPKRFPDDEAIARAAGAEDVLVQATVCESLELALSDTIFAVAISARHR
                     NLGPEPMQARQAAPEILAQAENGKVALVFGNETAGLSNAEVQRCQRTVFIPANPDYSS
                     LNLASAVQLLCYELRLAAFDGSPPVITKAVPFASPLATNDDVERFYAHLERVMIDTGF
                     LDPQQPRRLLPKLRRLFGRTELERDEINILRGILDAVEKRIDKQGADR"
     gene            2219..2971
                     /gene="cysE"
                     /locus_tag="PLD53_08380"
     CDS             2219..2971
                     /gene="cysE"
                     /locus_tag="PLD53_08380"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRC60970.1"
                     /translation="MFSRLREDIRSVFDRDPAARTAWEVLTCYPGVHAVIMHRLSHWL
                     WCLQLRWLGRLVSHLARFLTGIEIHPGATIGRRFFIDHGMGVVIGETAVIGDDVTLYH
                     GVTLGGTSWNKGRRHPTLENGVVIGAGAQVLGPITIGAQAKVGSNAVVVKSVPAGATA
                     VGNPARIIDTDLAQKREEMAGQMGFSAYALASNQDDPLAKAIHGLVDHAVETDRRIGV
                     LLKKLESSGVCLEDELATADKFDPNYLSKIVD"
     gene            3029..3520
                     /gene="iscR"
                     /locus_tag="PLD53_08385"
     CDS             3029..3520
                     /gene="iscR"
                     /locus_tag="PLD53_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HRC60971.1"
                     /translation="MRLTTKGRFAVTAMIDLALRSGEGPVTLAGISERQKISLSYLEQ
                     LFGKLRRCSLVDSVRGPGGGYCLARPGTQITVADIVRAVDETLDATQCGGKENCKDEE
                     RCMTHELWTTLNTKMYDYLTSVTLSDLVDSQLKKVGGDVSVLRDARRIGPNPRAKSTP
                     AFA"
     gene            3525..4682
                     /locus_tag="PLD53_08390"
     CDS             3525..4682
                     /locus_tag="PLD53_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRC60972.1"
                     /translation="MFAPVYLDHNATTPLEPAVREAMLPWLSEQCGNASSRHDYGRSA
                     RRAIDEARSQVASAIGAHFSEIVFTSNGTEANNLFVKGAAACMKPGLLAVSAIEHPSV
                     IRPAEQLVRRGWQLHKLAVENDGQVDAQSYRTALAGKPRLVSVMLANNETGVLQAVDA
                     LAAQARAAGALFHSDAVQALGKISVDFRSLNAAGVNALTLSAHKIGGPKGAAALVLDK
                     RVEIEPLIAGGGQERGLRSGTENVAAIVGFGVACELAVRNLAAVAPRLLALRDQLERG
                     LRAMGATLFGLGAGRLPNTTYFALDDVDGETLVSHLDRAGFAVASGAACSSANPEPSH
                     VLRAMGVAPELARGAVRVSLGAASTAEQVSDFLRTLQITVSKLLRLTAVAV"
     gene            4724..>5694
                     /locus_tag="PLD53_08395"
     CDS             4724..>5694
                     /locus_tag="PLD53_08395"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRC60973.1"
                     /translation="MLKLPIYLDYSATTPVDPRVAEKMIPYLVEKFGNPASRSHAFGW
                     EAEAAVEEARAEVAKLVNADPKEIVWTSGATESNNLAIKGAAHFYSGKGKHVITVKTE
                     HKAVLDTCRELERGGFEVTYLDVKESGLLDLDVFKAALRPDTILVSVMLVNNEIGVIQ
                     PIAEIGEICRGKGIIFHVDAAQATGKVAIDLTQLKVDLMSFSAHKSCGPKGIGALYVR
                     RKPRVRLEAQMHGGGHERGFRSGTLATHQIVGMGEAFRIAREEMGAENERIRMLRDRL
                     LKGLSDIEQVFINGDLEHRVPHNLNISFAYIEGESMLMAIKDLAVSSG"
BASE COUNT         1141 a         1562 c         1784 g         1207 t
ORIGIN      
        1 ctccatacgc ataactgctt tatatgttat acgaaggagg tgcgaattgg aaggtgtcgg
       61 agagtgatag tgcaaccacc agtatggagt agcaatcttg accggccgtt tgacagctga
      121 aattcaggcg ggcgattcaa ggtggacttg gttcaatggc cgcttacggg aaatcaggat
      181 ggtccagcgt ccgcctgagg gtgtcgaatg aagcgcgccc cgtaccatcc gtccttcgag
      241 ggagccgaag aatcaagcat gtcggctctg gtggaacgga tcatcgatag cggcaaggcg
      301 atctcgccct gaagcgttcc gagttggccg gcagcggaga agatgttata cgcgtagccg
      361 accggttgaa gccattcgcc ttgcatccag ccctggtcgt gcgaaaagct tgataacaca
      421 gcccggcaca acggccaatc aggcgccgag tcgggctgtg cggtggctgt cgacgacctg
      481 gagcaattgc gcgaaaacct tgggcgtgcc ggcaatcacg ttcccggtct ccagatagtt
      541 ctcgttgccg gaaaggtcgc cgatcaggcc gcccgcttct gaaaccagca gcgcgccacc
      601 ggcgatatcc cacggcgcaa gaccgaattc ccagaaacca tcgagccggc cgcaggcgac
      661 ataggcgaga tccagcgacg cggcacctgg gcgacgaatg ccggacgact tctgcatcag
      721 ctccttgagc gtgccgagat aggcgtcgat gtgctcgaag ctgtggtaag gaaaaccggt
      781 gccgatcaag gcatcttcaa gtttgaggca tttgctgacg cggacgcggc gatcattaag
      841 aaaggtgccg gcgcccttgc tggcagtgaa catttcgttg cgcatcgtgt cgtagacgac
      901 tgactgtgtt acctgaccca tgtgcgtcag tgcgatcgat atcgcatatt gcggaaaccc
      961 gtggatgtag ttggtcgtgc catcgagcgg atcgatgatc cactggtagt cgctgccggc
     1021 gcctcgcccg gcggtctggc ctgactcttc tgctagaatt ccgtactccg gataagcctc
     1081 gctgagcact tcgataattg cggcttcggc ggccttgtcc acttcggtaa cgaagtcgct
     1141 ctgacgcttg gtcgtcacct tgaggtgttc aagttcgttc gcagaacggt tgatgatctg
     1201 gctggcccgc cgcgcggcct tgatgatgat gttcagtgct ggatgcatgg ttttaaagaa
     1261 ctctacgggc ggggaagggt gccgggctgg cacctgattc ggcccgattc atttgcggag
     1321 cgcgtattct atatgaaccc tgccttggcg tctcttggtg ctcgttcccc tcatcgcttg
     1381 gtcacgccgc tcgacagggt gcatgtcgtg ctgtcgcatc cgagccatcc aggcaatatc
     1441 ggtgccgcgg cgcgcgcgat gaagacaatg ggcctgtcgc acctgacgct ggtcaatccg
     1501 aagcgctttc cggatgacga agccatcgcc cgcgcggcgg gtgccgaaga cgttctggta
     1561 caggcaacgg tttgcgagag tctcgaactt gctctttccg atacgatctt tgcggtggcc
     1621 atctcggcgc gtcatcgcaa cctgggtccc gaaccgatgc aggcgcgtca ggccgccccc
     1681 gaaattctcg ctcaagcgga gaacggcaag gtggcactag tgttcggcaa cgagacggcc
     1741 ggcctgtcga acgccgaggt gcagcgctgc cagcgtacgg tattcatccc ggccaatccg
     1801 gattattcct cgctcaatct tgcttcggcg gtgcaactgc tttgttatga gctgcgattg
     1861 gcggcgttcg acggcagtcc gccggtgatt accaaggctg taccctttgc ttcgccgctt
     1921 gcgaccaacg acgatgtcga gcgtttttac gcgcatcttg agcgcgtcat gatcgatacc
     1981 ggattcctcg atccgcagca gcccaggcgg ctgttgccaa aactgcgtcg gctgttcggt
     2041 cgcacggagt tggagcgcga cgagatcaac atcctgcgcg gcatcctcga cgccgtcgaa
     2101 aaaaggatcg acaagcaagg ggctgaccgc tgagacaccg ttcagcgtgc ttgaatcttg
     2161 accgaatcag tcgggtatta tagaatcgcc ccttttcgca gcaaggaata tcacttcaat
     2221 gttcagccgt ttgcgtgaag acatccgttc ggtttttgat cgcgatcccg ccgcccgtac
     2281 tgcgtgggag gtgttgacct gctatccggg ggtgcacgct gtgatcatgc atcgactgtc
     2341 gcactggctg tggtgccttc agttgcgttg gctggggcgc ctggtttcgc atctggcccg
     2401 gtttcttacc ggcatcgaga tccatcccgg ggcgacgatt ggtcgacgtt tcttcatcga
     2461 ccacggcatg ggtgtcgtga ttggcgagac cgcggttatt ggcgatgacg tgacgctcta
     2521 tcatggggtg actctcggtg gtacgtcgtg gaacaaggga cggcgtcacc cgacgctgga
     2581 gaacggcgtc gttatcggtg ccggagcgca ggttctcggg ccaattacga ttggtgcgca
     2641 ggccaaggtt ggttccaatg cggtggtcgt caagagcgtc ccggccggag ccacggccgt
     2701 ggggaacccg gcacgcatca tcgacaccga tctggcgcag aaacgcgagg aaatggccgg
     2761 ccagatgggc ttttcggctt acgcactggc gagcaatcag gatgaccctc tggcgaaggc
     2821 aattcacggc ttggtggatc atgccgtgga aaccgatcgg cgtattggcg tattgctcaa
     2881 gaagcttgag agtagtggcg tctgcctgga agatgagctg gctacggcag acaagttcga
     2941 tccaaattac ctgagtaaga ttgttgacta attcggggaa cttttcccat agttgacaga
     3001 actaatcaag tattaaccag gaggcactat gcgactgaca accaaaggac gttttgcggt
     3061 aaccgcgatg atcgatttgg cgctgcgtag cggcgaaggc cctgttacct tggccggcat
     3121 cagcgaacgg cagaagatat cgctctcgta tctcgagcaa ttgttcggaa aattgcgtcg
     3181 ctgcagtctg gtcgatagcg tgcgcgggcc cggaggcggg tactgtctgg cgcgacccgg
     3241 tactcagatc actgtcgccg acattgtgcg agccgttgat gagacgcttg acgcgaccca
     3301 gtgtggcggc aaggagaact gcaaggacga agaacgctgc atgacacatg agctgtggac
     3361 tacgctcaac actaaaatgt acgactacct gacctcggtg acgttgtccg atctggtcga
     3421 tagtcagctc aagaaggttg gcggtgacgt ctcggtgctc cgggatgcgc ggcgtatcgg
     3481 cccgaatccg cgcgccaaat cgaccccggc ttttgcctga cagcatgttt gcgcctgtct
     3541 atcttgatca caacgcaacc acaccgctgg aaccggcggt gcgggaggcg atgttgccct
     3601 ggctttccga gcagtgtggc aatgcgtcca gtcggcacga ctacggccga tctgcacggc
     3661 gcgctatcga cgaggcgcgt agtcaggtgg cctcggcaat cggcgcacat ttcagtgaaa
     3721 tcgtattcac cagcaacgga acggaagcga ataacctgtt tgtcaaaggt gcggccgctt
     3781 gcatgaagcc tggtctgctg gcggtcagcg cgatcgagca tccgagcgtg atcagacccg
     3841 cagagcaatt ggttcggcgc ggttggcagc tgcacaagct tgccgttgag aacgatgggc
     3901 aggttgatgc gcagagttac cggacggcat tggcaggaaa gccaagattg gtctcggtga
     3961 tgcttgccaa caacgaaacc ggcgtgttgc aggccgtcga tgcgctggcc gcgcaggccc
     4021 gagcggcggg tgcattgttt cactccgatg cggtgcaggc actgggcaag atcagcgttg
     4081 atttccgcag cctgaatgcg gcaggcgtca atgcgctgac gctctctgcg cacaagattg
     4141 gcggaccaaa gggagccgct gcgctggtac tcgacaagcg tgttgaaatc gaaccgctta
     4201 ttgccggtgg tggtcaggag cgcggcttgc ggtccggaac ggagaatgtc gccgctatcg
     4261 tcggattcgg tgtcgcttgt gaattggccg tgcgaaatct ggccgcggta gcaccgcgct
     4321 tgctcgcgtt gcgagatcaa ctggaacgcg gcttgcgcgc aatgggtgcg acgcttttcg
     4381 gtcttggcgc cgggcgtctg cccaacacaa cctactttgc gctggacgat gtggatggcg
     4441 aaacgctggt cagtcatctg gaccgcgccg gatttgccgt ggccagtggt gccgcgtgtt
     4501 ccagcgccaa tccggagcca tcgcatgtgc tgcgcgccat gggagttgcg cccgaacttg
     4561 cgcgtggcgc cgtgcgcgtc agcctgggtg ccgccagtac ggccgagcaa gtgagcgatt
     4621 ttttaagaac attgcagatc acagtatcca aactgctgcg actgaccgct gttgcggttt
     4681 aacccatgaa tattgaaatc atgaaccatc tggagtccgc gaaatgctga aactgccgat
     4741 ctatcttgac tattcggcaa ccacaccggt cgatccgcgc gtggcggaaa aaatgatccc
     4801 ttatctcgtc gaaaagttcg gcaaccccgc ctcgcgttcg cacgccttcg gctgggaggc
     4861 tgaagccgcg gttgaggagg cgcgtgccga agtcgccaaa ctggttaatg ccgaccccaa
     4921 ggaaatcgtc tggacttcgg gtgcgaccga gtcgaacaac ctggcgatca aaggggcggc
     4981 gcatttctat tccggcaagg gcaagcatgt cattaccgtc aagaccgagc acaaggccgt
     5041 tctggacacc tgccgcgaac tggaacgggg aggttttgaa gtcacgtatc tcgatgtcaa
     5101 ggaaagcgga ctgctcgatc ttgacgtatt caaggccgca ctgcgtcccg acaccattct
     5161 cgtgtcggtg atgctggtca ataacgagat cggcgtcatt cagccgattg ccgaaattgg
     5221 cgaaatctgc cgcgggaaag gcatcatttt tcatgtcgat gcagctcagg cgaccggcaa
     5281 ggtagcgatc gatctgacgc agttgaaagt tgacctgatg agtttcagtg cgcacaagtc
     5341 ctgcggccca aagggcatcg gagcgctcta tgtgcgccgc aagccacgcg ttcgtctgga
     5401 ggcgcagatg catggcggtg gccacgaacg cggtttccgt tcgggaacat tggcaacgca
     5461 tcagatcgtc ggtatggggg aagccttccg cattgcgcgt gaggagatgg gcgccgagaa
     5521 cgaacggatc cgcatgctgc gcgatcgact gctcaagggc ttgtccgata ttgagcaggt
     5581 gtttatcaac ggtgatctgg agcatcgtgt tccgcacaac ctgaacatca gctttgccta
     5641 tatcgaaggc gaatcaatgc tcatggcgat caaggatctc gcggtgtcgt cggg
//
LOCUS       DAORPM010000160         5676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3927_length_5676_cov_9.602740, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000160 DAORPM010000000
VERSION     DAORPM010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3927_length_5676_cov_9.602740"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2475)
                     /gene="cphA"
                     /locus_tag="PLD53_08400"
     CDS             complement(<1..2475)
                     /gene="cphA"
                     /locus_tag="PLD53_08400"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRC60974.1"
                     /translation="MEVSRIRALRGPNLWSRHTAIEAIITCAEIECFIDRIPGFEARL
                     RERFPEIALLQPTISDEVIAMAHALEFAALGLQAQAGCPVTFSRTAQTLEPGVYQVVV
                     EYSEEAVGRLAFELAQELCQAAIDDQPFDLDDALRRLRELDEDVRLGPSTGSIVYAAV
                     ARGIPYRRLTEGSMVQFGWGSKQRRIQAAETDLTSAVAESIAQDKELTKTLLHAAGVP
                     VPAGRPALSAEDAWAAACEIGCPVVVKPQDGNQGKGVAVNLSTREQIESAYAVAAEIS
                     DEVLIERFIPGHDYRVLVVGNKLIAAARREPPHVTGDGTHTVRELVELVNSDPKRGVG
                     HATSLTRISFDNIALARLATEGLTSDSIPARGARVVLRDNANLSTGGTATDVTDDVHP
                     EFAARAVAAAQTIGLDIAGVDIVCNSVLRPLEEQGGGIVELNAAPGLRMHLQPSYGKG
                     RAVGEAIIANMFADGDDARIPVVAVAGTNGKTTTVRLIASIFSSLGLRVGMTSTDGVY
                     VEGRRIDTGDCSGPKSARNVLLHPDVDAAVLETARGGVLREGLGFDYCDVAVVTNIGK
                     GDHLGLSYISTVDELAVVKRVIVRNVRPDVGVAVLNAADPMVARMADACPGKVTFFAL
                     DRNHPVMAMHRAQGKRVVYLDADSIVATEGGFEQRIPLAEIPVTANGAIGFQIENAMA
                     AAGAGWALGIDWTIIRAGLADFVNDAETAPGRFNLFNYRGATLIADYGHNPDAIMALV
                     GAIDNMPATASTRRSVVISGAGDRRDEDIRQQTEILGDAFDQVVLYQDQCQRGRADGE
                     VLALLQLGLKNARRTTDIREIHGEFVA"
     gene            complement(2557..4728)
                     /gene="cphA"
                     /locus_tag="PLD53_08405"
     CDS             complement(2557..4728)
                     /gene="cphA"
                     /locus_tag="PLD53_08405"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRC60975.1"
                     /translation="MKTKDIKFLEIRHLRGPNIWTYRPVIEAVIDIGELEDYPSNTLP
                     GFVDRLKGFLPSLVEHRCSYGERGGFLRRLDEGTWPGHILEHVSLELQNLAGMPGGFG
                     KARETSIRGVYKVVIRAWHEEVTRACLEAGRELVLAAIHDTTFDVDANVERLRDMAER
                     KLLGPSTGCIVEEATSKARRIPAIRLLPTGNLVQLGYGIRSRRIWTAETDRTSAIAET
                     ISRDKDLTKSLLQSCGVPVPEGRLVNSPADAWDAAEDIGIPVVVKPYDGNHGRGVFTN
                     LMTRDEIEAAYTAAIEEGSGVIVERFVRGSEHRLLIVGGKLVAAARGETATVVGDGKS
                     TINELIDLQINIDPRRGAAEEFPLDVIDLSKNSVPLHEITRQGFTPDSVPPAGREVLV
                     VRTGNHTCDITDKVHPSVADSASLAARIVGLDIAGVDLVCEDISRPLAEQHGAIVEVN
                     AGPGLLMHIKPATGEARPVGRAIVEHLFPNGEDGRIPVVGVTGSFGKTIVCRLIARLL
                     TLSGKHTGLACSDGLFLDHRQVDKSDCAQWEAATRILKNRAIEAAVFENGSDTILSEG
                     LAYDRCQVGVITNVDVERHTGRYYIETAEQVANVLRTQIDLVLPKGVALLNAGEPMLV
                     DMAPLCDGEVIYFALDPQLPVVAEHRAQGKRAVIVRDGQIQLASGKDELPLTRLEHIP
                     VTKSGQSTHQVENVLAAVGAAWALGISPDVMRTGIETFFVE"
     gene            complement(4865..5329)
                     /locus_tag="PLD53_08410"
     CDS             complement(4865..5329)
                     /locus_tag="PLD53_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HRC60976.1"
                     /translation="MNDYRLQRDAFGRLVLTDADGESHAGVVPVRAFPIAAPDQGIAL
                     VDPHGHELAWIDRLTDLPDELRALVEAELASREFMPVISRIVSVSSFATPSTWRVKTD
                     HGETDFVLKGEEDIRRLALPALLIADSHGIHYLIHDRYALDHHSRKILDRFL"
     gene            complement(5335..>5676)
                     /locus_tag="PLD53_08415"
     CDS             complement(5335..>5676)
                     /locus_tag="PLD53_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60977.1"
                     /translation="IARALLIDPKILILDEATSSVDTTTEKEIQKALDNLVRGRTTIA
                     IAHRLSTLRDANRLVVLDRGRIVEVGNHDDLMAQEGHYYRLYQAQARNVDTEDDSARI
                     EISGKDEGDRE"
BASE COUNT         1088 a         1669 c         1749 g         1170 t
ORIGIN      
        1 ggctacgaac tcgccatgaa tttcccggat atcggtggtg cgcctggcat ttttcagacc
       61 aagctgaagg agcgccagca cttcgccgtc ggcacgtccg cgttgacact gatcctggta
      121 aagaacgacc tgatcgaacg catcgccgag gatttcggtt tgctggcgga tgtcctcatc
      181 gcgccggtct cccgcgccgc tgatgactac cgagcgtcgc gtgctcgccg tggcaggcat
      241 attgtcgatg gctccaacca gcgccatgat cgcgtccggg ttgtggccgt aatcggcgat
      301 cagtgtggcg ccacggtagt tgaacaggtt gaagcggccg ggagccgttt ctgcgtcgtt
      361 cacgaagtcg gccaggccgg cgcgaatgat tgtccagtcg atgcccagcg cccagcccgc
      421 gcccgcagca gccatcgcgt tttcgatctg gaagccgatg gcgccattgg cggtgaccgg
      481 gatttccgcc agcggaatgc gctgctcgaa gccgccctcg gtggcgacga tagaatcggc
      541 gtccaaataa accacgcgtt tcccctgagc ccggtgcatg gccattaccg ggtggttgcg
      601 atcgagggcg aagaaggtca ctttgccggg gcacgcgtcg gccatgcgcg caaccatcgg
      661 gtcggccgcg ttgagcacag caacgccgac gtccggcctg acgttgcgga cgatgacgcg
      721 ctttacgacg gccagttcgt ccacggtgct gatgtatgac aggccaaggt ggtctccctt
      781 gccgatattc gtgactaccg ccacatcaca gtagtcaaaa cccaagcctt cgcgcagcac
      841 gccaccgcgt gcggtctcca atacggcggc atcgacatcg ggatgcagaa ggacatttct
      901 ggcgcttttc gggccgctgc agtcgccggt gtcgatgcgc cgtccttcga cgtatacacc
      961 atccgtactg gtcatgccga cacgcaggcc gaggctggag aaaatactgg cgatcaggcg
     1021 taccgtggtg gtcttgccgt tggttccggc cactgcaacc acggggatgc gcgcatcgtc
     1081 tccatcggca aacatgttgg cgatgatcgc ttcgccaacc gcacgcccct tgccgtacga
     1141 cggttgcagg tgcatgcgca gaccgggtgc ggcattgagt tcgacgatgc cgccgccctg
     1201 ttcttcgagc gggcgcagga cgctgttgca aacaatgtct acgccggcaa tgtcgaggcc
     1261 gatggtttgc gcggcagcca cggcgcgtgc ggcaaattct gggtggacgt cgtcggtgac
     1321 gtcggtcgcc gttccgccgg tcgatagatt ggcgttgtcg cgcagtacca cacgggcgcc
     1381 tctggccggt atagagtcag aagtcaagcc ctcggttgct aggcgggcta gggcgatgtt
     1441 gtcaaagctg atccgcgtca gtgacgtcgc atgaccaacg ccgcgtttcg gatcactgtt
     1501 gaccagctcg acgagttccc ggacggtgtg cgtgccgtcg ccggtcacat gcggcggttc
     1561 acgccgggcg gcggcgatca gcttgttgcc gacgaccagc acgcggtagt cgtgaccggg
     1621 aatgaaacgc tcgatcagta cctcgtcgct gatctcggcc gccacggcgt aagccgattc
     1681 gatctgctca cgcgttgaca gattgaccgc tacgcccttg ccctgatttc catcctgtgg
     1741 cttgacgaca accgggcaac cgatttcgca ggcggcggcc caggcatctt cggcactcag
     1801 tgccggccgg ccggccggca ccggaacgcc ggcagcgtgc agcaaggtct tggtcaactc
     1861 cttatcctgc gcgatggact cagccaccgc gctggtgagg tcggtttcag cggcctgaat
     1921 gcggcgctgt ttgctgcccc atccgaattg caccatgctg ccttcggtca gccggcggta
     1981 gggaatgccg cgtgcgacag cggcgtaaac gatggagccg gtgcttggcc cgagacgcac
     2041 gtcctcgtcg agctcgcgca ggcgacgcaa ggcgtcgtca agatcaaagg gctggtcgtc
     2101 aatggccgcc tggcagagtt cctgtgccag ctcgaaagcg agcctgccta ccgcttcctc
     2161 actgtattcg acaaccacct ggtaaacgcc gggctccagc gtttgcgccg tgcggctgaa
     2221 cgtcaccggg cagcccgcct gtgcctgtag tccgagcgcg gcaaattcaa gagcgtgcgc
     2281 catggcgatg acctcatcgc tgatggtcgg ctgaagcaac gcaatctcgg ggaagcgttc
     2341 gcgcaaacgc gcttcaaagc caggaatgcg atcaatgaaa cactcgattt cggcgcaggt
     2401 gatgatcgcc tcaatcgccg tatgccggct ccacagattg gggccgcgca aggctcggat
     2461 gcgtgatact tccataaacg gattcctctt ggttttcggt tgccggggat tttttggcat
     2521 ccgtgctgag gttaatgttt cttcctgctt gaatgattac tctacaaaaa aagtttctat
     2581 gccggtgcgc atgacatcgg gcgaaatgcc gagtgcccag gcggctccga cggcggccag
     2641 aacgttttcg acctgatgag tcgactgccc gcttttggtc acgggaatgt gttcgagccg
     2701 ggtcagcggc aattcgtcct tgccgctggc caactggatc tgcccgtcgc ggacgataac
     2761 ggcgcgtttt ccctgcgctc ggtgttcggc gacgaccggc aattgcgggt caagcgcgaa
     2821 gtaaatgact tctccatcgc acagcggagc catgtcgacc agcatgggtt cgccggcgtt
     2881 gagcagcgct acgcctttgg gcagcaccaa gtcgatttgc gtgcgcagaa cgttggctac
     2941 ctgctcggcc gtttcgatgt agtagcgacc ggtgtgacgt tcgacgtcga cattcgtaat
     3001 gactccaacc tgacagcggt cataggccag tccctcgctg aggatagtgt cactgccgtt
     3061 ttcgaagacg gccgcttcaa tggcgcggtt cttcaaaatg cgtgtagcgg cttcccattg
     3121 ggcacaatcg cttttatcga cttgccggtg atcaagaaag aggccatcgc tgcaggccag
     3181 cccggtgtgc ttcccggaaa gcgtcagcaa tcgcgcgatg agacggcaga caatggtctt
     3241 gccgaagctg ccagtgacgc caactaccgg gatgcggcca tcttccccat tcgggaaaag
     3301 gtgctcgacg atagcgcggc cgaccggacg cgcctcaccg gtggctggct tgatgtgcat
     3361 cagcaatccc ggtccagcat tcacttcaac gatggcgcca tgctgttcag caagcggacg
     3421 ggaaatatct tcacagacca gatcaacacc ggcgatatcg agaccgacaa ttcgcgccgc
     3481 cagagaagcg ctgtcagcga cactgggatg aaccttgtcg gtgatgtcgc aggtatggtt
     3541 gccggtgcgt acaacgagca cttcacgacc ggcgggcgga accgaatccg gtgtgaaacc
     3601 ctggcgcgta atttcgtgca gcggtaccga attcttactg agatcgatga catcaagcgg
     3661 gaactcttcg gctgcgccgc ggcgcgggtc aatgttgatc tgaagatcga tcagctcatt
     3721 gatggttgat ttgccgtcac cgacaactgt tgccgtctca ccacgagcgg cggcgaccag
     3781 tttgccgccg acgatcagca agcggtgttc ggagccgcgt acaaagcgtt cgacaatgac
     3841 accgctgcct tcttcaatgg ctgccgtata agccgcttct atttcgtcac gagtcatcag
     3901 attggtaaat acgccgcgcc cgtgattacc gtcgtagggt ttgacgacaa ccgggatgcc
     3961 gatgtcttca gccgcgtccc aggcgtcggc cgggctattg accagacgtc cttcgggaac
     4021 agggacgccg caggactgaa gcaatgattt cgtcagatcc ttgtcgcgcg agatggtctc
     4081 ggcaatggcg ctggtgcggt cggtttcggc cgtccagatg cgacggctgc ggatgccgta
     4141 acccagttga acgaggttgc ccgtgggtag cagacggatt gccggaatgc gccgcgcctt
     4201 ggacgtggct tcctcgacga tacacccggt gctcggtcca agcagctttc gctcggccat
     4261 gtcgcgcaag cgttcaacat tggcatcgac gtcaaaggtc gtatcgtgaa tcgcggccaa
     4321 taccagttcc cggccggctt ccaggcaggc acgggtcacc tcttcatgcc aggcgcggat
     4381 gaccacttta tacacgccgc ggatcgacgt ttcgcgtgcc ttgccgaatc ctccgggcat
     4441 gccggcgagg ttttgcagtt cgagcgagac gtgctcaaga atgtgtcccg gccaggtgcc
     4501 ttcatcaagc cggcgcagaa aaccgccgcg ttcgccgtag ctgcagcggt gctcgaccag
     4561 tgagggcaga aatcctttca gacgatcaac aaaaccgggc agggtgttcg atggataatc
     4621 ttcaagttcc ccgatatcaa tgacagcttc aatgaccggc cggtaggtcc agatgttcgg
     4681 tccgcgcagg tggcggattt caaggaattt gatgtctttc gttttcatat tgtccgtatt
     4741 ggcaacagaa gaggcgagcc ggtggcatga ggagagcacg tatcgtacga ataacgctgt
     4801 ccatgatatt cggctgacgc cgcagcgaaa tattcagccg gcttgtttat cggattgaaa
     4861 agattcacag gaatcgatcc agaatcttgc ggctgtggtg gtccagcgca tagcgatcat
     4921 ggatcaggta atgaatgccg tggctatcgg cgatgagcag agcaggcaaa gccagacggc
     4981 gaatatcctc ttctcccttc agaacgaagt cggtttcgcc atgatcagtt ttgacccgcc
     5041 aggtgctggg tgttgcgaag ctcgaaacgc tcacaatgcg gctgatgacg ggcatgaatt
     5101 cgcggctggc aagttccgcc tcaaccagcg cgcgcagttc gtcgggcagg tcggtcaatc
     5161 ggtcgatcca ggccagctcg tgaccgtgcg ggtcgaccag cgcgatacct tgatccggtg
     5221 cggcgatcgg gaaagcgcga accggaacca cgccggcatg agattcgccg tcagcatcgg
     5281 tcaataccag acgcccgaaa gcatcgcgtt gcagccgata atcattcatc ctggtcactc
     5341 gcggtctcct tcgtccttgc cggaaatttc aatccgggcg gaatcgtcct cggtgtcgac
     5401 gttgcgcgcc tgagcctgat aaaggcggta gtagtgccct tcctgcgcca tcagatcgtc
     5461 gtgatttcct acttccacga tacggccgcg atcgaggaca accagacggt tggcgtcgcg
     5521 cagggtggac aagcggtggg cgatggcgat ggttgtacgg ccgcgaacga ggttgtcgag
     5581 cgccttctgg atttcctttt cagtggtggt atcgaccgac gaggtggctt cgtcaagaat
     5641 cagaatcttc ggatcgatca acaaggctct ggcaat
//
LOCUS       DAORPM010000161         5652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3955_length_5652_cov_6.379310, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000161 DAORPM010000000
VERSION     DAORPM010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3955_length_5652_cov_6.379310"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2520)
                     /locus_tag="PLD53_08420"
     CDS             complement(<1..>2520)
                     /locus_tag="PLD53_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRC60978.1"
                     /translation="YIVGIDLGTSNTVVAYAAPGKQHVELFEVEQLVGPGQVAARPAL
                     PSVRFHPAPGEISPGDLQLPWTEADSADQVLTGRFALDLGAQVPGRLVASAKSWLSHA
                     SVDRMAPILPWGADDDVDKVSPVDASASYLAHVRAAWLHRFPHAPLEKQQIILTVPAS
                     FDEGARALTLEAAKQAGLNNLRLLEEPQAAFYDWLYRHQHCLADELADTKLLMVCDVG
                     GGTTDLTLIQVAMRDGDPELTRIGVGNHLMLGGDNMDLSLAHLVESRLPASEGKLTAA
                     RFSQLVARCRAAKELLLAPDAPESVPLTLLGGGSRLIGGARTIELKREEVRQMIVDGF
                     LPRVAPDEPILQRRAAIIEFGLPYPADPAITRHVADFLKRHAHASREALADDSSGQRM
                     PVPDTLLLNGGVFRSEAIAERLQDTLGDWRGDRLRVLHNDNPDVAVARGAVAYALVRQ
                     GQGQRIGGGSARSYFLVLEGEGEEERGICILPRGSEEGHEVQLPERIFSLRLGQPVRF
                     SLVSTVADNRYQPGEIAILTGEDWILLPPIATVVQAPPGNTQRDVRVKLISTMTEIGT
                     LELHCVNLEEASRRWLLEFQLRGVGDAGGDGREPGDCVVSAEALPPNFPDAIQCIESI
                     FGSRSLAAEKTEMRKQVRHLRGQLEHLLGKRENWDMPLLRALFDALMQRARRRRRSAE
                     HERLWLNLTGYCLRPGLGYALDEWRVEQLCQLLPQGIEYAGESQNWSEWWTLWRRAAG
                     GLPTAIQEKLLDELSPSLLAEAAKRRNRLMTAIAGSQDDMLRLVASLEGLPIERKVEI
                     GDWLLGRLRKSSVKALGWWVIGRIGARESLYGNAHQVVPPEV"
     assembly_gap    2521..2620
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2621..4434)
                     /locus_tag="PLD53_08425"
     CDS             complement(<2621..4434)
                     /locus_tag="PLD53_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRC60979.1"
                     /translation="MNTLNKMNDPKFSIGIDLGTTHCALSYVDISQCDGEQVTQSVLP
                     IPQLTGPGSVESPGLLPSFIYLPHADELAAGDLSLPWTAEQDFAIGEMARSRGAATPI
                     RLVSSAKSWLCHAGVDRRAAILPNDAPPEVPHLSPFEASQRYLAHLRAAWDQSHPDAP
                     FVDQDIVVTIPASFDPAARELTAEAAKEAGCSHMILLEEPQAALYSWIQKSAGGWRKM
                     VRPGEIILVVDVGGGTSDFSLIAVLEQEGVLELHRVAVGEHILLGGDNMDLALAYAVA
                     GKLSTQGTKLDAWQLRGLTHACRTAKETLLNDVQITAAPLVIASRGAKLIGGSIRSEL
                     TREELTATLLEGFFPAVSIADHPQGRGRAGLTQLGLPYAQDAAITRHLAAFLARQVAA
                     TNDLEGFTGQLPAGATFLHPTAVLFNGGVFKSELLAQRTLASLNSWLAGEGAPAARSL
                     EGADLDLAVARGAAYYGYVRRGQGVRIRGGSARSYYVAIESSMPAVPGMQPPVQALCL
                     APFGMEEGSEAALSSQEFGLVVGEQVRFRFFGSSVRRQDQVGTLLDDWEPDELQELDE
                     IQATLPSEGREAGEVVRVRLQARLTEAGTLELEALPHNG"
     gene            complement(4440..5003)
                     /locus_tag="PLD53_08430"
     CDS             complement(4440..5003)
                     /locus_tag="PLD53_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HRC60980.1"
                     /translation="MKQDTPNFLSRISIAFGSFFSIISDSQFAAKVQALRTGAQVTEA
                     SASAPAAEKPVVIPMRETAPEAALQLLGLLQREARFIDFIQEDVAAYQDADIGVAARV
                     VHDGCRKVLRDNFTISPVRDEAEGSRITLPEGFDAAAIRVTGNVVGQPPFTGSITHRG
                     WRVVETRLPKLADGHELRVIAPAEVEL"
     gene            complement(5392..>5652)
                     /locus_tag="PLD53_08435"
     CDS             complement(5392..>5652)
                     /locus_tag="PLD53_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC60981.1"
                     /translation="VATVAGAVGGGYAGNKIQKNMQDKDVVTTTEQRCKTVYDTSERI
                     VGYDVSYRLEGKQGMVRMDHNPGQQIPVKDGQLVLNGPVATK"
BASE COUNT         1070 a         1735 c         1675 g         1072 t
ORIGIN      
        1 gacctcgggc ggcacaactt gatgcgcatt gccgtaaagc gactctcggg ctccgattcg
       61 gccgataacc caccaaccca gcgcctttac agacgatttg cgcagtcttc caagcagcca
      121 gtcgccgatt tccacctttc tttcgatggg caacccttcg agcgaagcga cgaggcgcag
      181 catgtcatcc tgacttccgg caatcgcagt catcaggcgg ttacgccgct tcgctgcctc
      241 agccagcagc gaggggctca gttcgtccag cagtttttcc tggatggctg tcggcaagcc
      301 gcccgctgcg cgtcgccaca aggtccacca ttccgaccag ttctggctct cgcctgcgta
      361 ctcaatgcct tgcggaagga gttgacacaa ttgctcgacg cgccactcgt cgagggcgta
      421 gccgaggccg gggcgtagac agtaaccggt gagattgagc cacaagcgtt catgctcggc
      481 cgaacgccga cgacggcgtg cgcgctgcat cagagcgtcg aatagcgccc gcaagagagg
      541 catatcccag ttctcacgtt tgcccagcag atgttccagc tggccgcgca agtgccgtac
      601 ttgcttgcgc atttccgtct tttccgctgc cagagaacgc gatccgaaaa tgctttcaat
      661 acactgaatt gcatcgggaa agtttggcgg caaggcctcc gcagaaacca cacaatcgcc
      721 cggttcacgg ccatcgccgc ctgcgtcgcc cacgccacgc aactggaatt cgagcagcca
      781 gcgtcgactg gcttcttcca ggttgacgca gtgcagttcc agcgtgccga tttcggtcat
      841 cgtgctaatc aatttcacgc gaacatcgcg ctgcgtattg ccggggggcg cctgaacgac
      901 ggtggcaatt ggcggcagca aaatccagtc ctcgcctgtc aggatggcga tttcgcccgg
      961 ctgatatcgg ttatcggcta ccgtagaaac gaggctaaag cgaaccggct gacccagccg
     1021 cagactgaaa atgcgctccg gcaactgcac ttcgtgccct tcctcgctgc cacgcggcag
     1081 gatgcaaatg ccacgttcct cctcgccctc cccttcgagc acgagaaaat aactccgtgc
     1141 cgatccacca ccgatccgct gcccttgacc ctggcgaacc aacgcgtagg cgactgcacc
     1201 acgcgccacc gcgacgtcgg ggttgtcgtt gtgcagaact cgcaacctat cgccacgcca
     1261 gtcgccgagc gtgtcttgca gtcgctcggc aatcgcctcg gagcgaaaca cgccaccgtt
     1321 gagaagcagc gtatcgggca ccggcatccg ttgcccgctg ctgtcgtcgg ccagagcctc
     1381 ccgtgacgca tgggcatgcc gcttcaggaa atcggcgacg tggcgagtaa tcgccgggtc
     1441 ggcgggatac ggcaagccga actcgatgat tgcggcgcgt cgctgcagta tcggctcatc
     1501 cggcgcgaca cgagggagaa aaccgtcgac aatcatttgc cggacttctt cccgcttcag
     1561 ctcgattgtc cgtgcgccgc cgatcagcct ggagccgccg cccagtagcg tcaatggaac
     1621 cgactccggc gcgtctggcg ccagcagcaa ttctttcgcc gcacggcaac gcgccacgag
     1681 ttgcgagaag cgcgctgctg tcagcttgcc ctcgctggcc gggagccgtg attcaaccag
     1741 atgggcgagc gagagatcca tgttgtcgcc gccgagcatc aagtggttgc cgacaccgat
     1801 tcgggtcagt tccgggtcgc cgtcgcgcat cgccacctgg atcaaggtca gatcggtcgt
     1861 gccgccaccg acatcgcaaa ccatcagcag cttggtatca gcaagttcat ccgcaaggca
     1921 gtgctgatgg cgatacagcc agtcgtagaa ggccgcctgc ggctcttcga gtagtcgtag
     1981 attgttcaga cccgcctgtt tcgcggcttc cagcgtcagg gcacgcgcgc cttcgtcgaa
     2041 agaagcggga acagtcaata tgatctgctg cttttccagc ggcgcatgcg ggaagcggtg
     2101 cagccaggcg gcgcggacat gggcgagata gctcgcactg gcgtcgaccg gcgagacctt
     2161 gtcgacatca tcgtcggcgc cccatggcag gatgggcgcc atgcggtcta ccgaagcatg
     2221 cgagagccag ctcttggcgc tggccaccag ccgccccggg acctgggccc cgaggtcaag
     2281 cgcgaagcga ccggtaagca cctgatctgc actgtccgcc tctgtccatg gcaattgcag
     2341 atctcccggg ctgatttctc ccggcgctgg atgaaaacgc acggacggca gcgcgggtct
     2401 cgcggcgacc tggcctggcc cgaccagttg ctcgacctcg aacagctcga cgtgctgttt
     2461 cccaggggct gcgtaggcaa caaccgtgtt gctggttccg aggtcaatgc cgacgatgta
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgttgtgcg gcaatgcttc
     2641 aagttcgagc gtcccggctt cggtcaaacg tgcctgaagt ctgacgcgaa cgacctcacc
     2701 cgcctcgcgg ccttcgctgg gcagggtggc ctgaatctca tcgagttcct gaagctcatc
     2761 gggctcccag tcatcgagca gggttcccac ctgatcctga cggcgcaccg acgagccgaa
     2821 gaagcgaaag cgaacctgct cgccaacgac caggccgaat tcctgactcg acagggcggc
     2881 ttcactgccc tcctccattc cgaagggagc caggcacagc gcctggaccg gtggttgcat
     2941 ccccggtacg gcgggcatcg acgattcaat ggccacatag taagaacggg ccgagcctcc
     3001 ccggatacga acgccctgcc cgcggcgcac gtagccgtaa tacgcggcac cgcgcgcgac
     3061 ggccagatca aggtcggcgc cttcaagcga gcgcgcggcc ggcgcacctt cgccggcgag
     3121 ccatgaattg agcgacgcca gcgtacgctg ggcaagcaat tcggacttga agacgccgcc
     3181 gttgaacagc acagcggtcg gatgaagaaa cgtcgcgccg gccggcaact ggccggtgaa
     3241 tccttcaaga tcgtttgttg cggcgacctg gcgagccaga aatgcggcaa gatgacgggt
     3301 tatcgcggca tcctgtgcgt acggcaggcc gagctgggtc agtccggcgc ggccgcgacc
     3361 ctgcggatgg tcggcaatgg acaccgccgg gaaaaaacct tcaagcaacg tggccgtgag
     3421 ctcttcgcgc gttagttcgc tgcggatcga cccgccgatc agtttcgcgc cacgactggc
     3481 gatgaccaag ggcgcggcgg tgatctgtac atcgttgagc agcgtttcct tggcggtacg
     3541 gcatgcatgc gtcaagcccc ggagctgcca ggcatcgagc ttggtgcctt gtgtggacag
     3601 ctttccggca acggcgtacg ccagagccag atccatgttg tcaccgccaa gcaggatgtg
     3661 ttcgccgact gcaacgcggt gcaattcgag aaccccttcc tgctcaagca cggcgatcag
     3721 ggagaaatcg ctggttccgc cgccgacgtc gacaaccaga atgatttcac ccggccgcac
     3781 catcttccgc caaccgccgg cgcttttctg aatccagctg taaagagcgg cctgcggttc
     3841 ttcgagaagg atcatgtgtg aacagcccgc ttctttggcc gcttccgctg tcagttcccg
     3901 cgctgccgga tcgaaagaag ccggaatcgt gacgacaata tcttgatcaa caaagggggc
     3961 atcagggtga gactgatccc aggcggcgcg caggtgcgcc agataacgtt gcgaggcttc
     4021 gaacggcgac aggtgcggca cttccggagg cgcgtcgttg ggcagaatgg ccgcgcgccg
     4081 atcgacgcca gcgtggcaga gccagctctt ggcgctcgat accagtcgaa tgggtgttgc
     4141 cgcaccacgg ctacgggcca tttcgccaat cgcaaaatcc tgctccgcag tccacggcag
     4201 ggatagatcg ccggccgcca gctcgtccgc atgcggaaga taaatgaacg atgggagcaa
     4261 gcccggcgat tcgaccgaac cgggaccagt cagctgcgga attggcaata cgctctgggt
     4321 tacctgctcg ccatcgcact ggctgatatc aacgtaggaa agagcacaat gcgtcgttcc
     4381 cagatcgatg ccgatcgaaa atttcggatc attcatctta ttcaacgtgt tcatttcgct
     4441 catagttcca cctctgcagg cgctatgacg cgaagttcat gaccatcggc aagcttgggc
     4501 aggcgcgttt cgaccactcg ccagccgcgg tgagtaatac tgccggtaaa gggcggctgg
     4561 ccgacaacgt tgccggtcac acgaattgcg gccgcgtcga aaccttcggg cagcgtgatc
     4621 cggctgcctt ccgcttcgtc acgtaccggg ctgatcgtga agttgtcgcg cagtaccttg
     4681 cgacagccgt catgcactac gcgcgcggca acaccgatgt cggcgtcctg atatgcggcg
     4741 acgtcttcct ggatgaaatc gatgaatcgc gcctcgcgct gcaacaggcc gaggagctgt
     4801 agcgccgcct ccggtgccgt ttcacgcatt ggaataacga cgggtttttc agccgcagga
     4861 gcagaagccg atgcttcggt cacttgggcg ccggtacgca aggcctggac cttggcggcg
     4921 aactggctgt cggaaatgat tgaaaagaag ctgccaaaag caatggaaat tctgctcagg
     4981 aagtttgggg tatcttgttt catgggatgt tcctggtatg agtcgctgcc gctaaagcag
     5041 cgcggtgatg tgtttttgag ttcgtctgcg agagcacttg gcttcgtatg cgagatgtgc
     5101 cagttgccgg atctgcttcg aagccgggga aagtatccca agcgtgcgct caagtagtcg
     5161 cagggcgagc tgcacggacg atgcgccaca cctgtgggtg atgacgtcta gctagtctgg
     5221 gagtagccca ggtgcgtcag ccgcggcgca atataaacca ttttcttgct ttcgggtagg
     5281 tgtcacgtgg cggaatcagg gcaatctcct tctcgaggcg tgacctgctg gaccccgcgc
     5341 gccttcacct ttcgtgccga aatccgccgc gtggcttcgg tgacggcaag attacttcgt
     5401 agcgactggt ccgttgagta ccagttgacc gtcctttacg gggatttgct ggcccgggtt
     5461 gtgatccatg cgtaccatgc cctgcttgcc ttccaagcgg tagcttacgt cgtagccaac
     5521 gattctttcc gaggtgtcgt agaccgtctt gcaacgctgt tcggtggtcg tgacaacgtc
     5581 tttgtcctgc atgttctttt gaatcttatt gcccgcatag ccgcccccga cggcccccgc
     5641 tacagtggcc ac
//
LOCUS       DAORPM010000162         5635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_3974_length_5635_cov_17.167384, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000162 DAORPM010000000
VERSION     DAORPM010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3974_length_5635_cov_17.167384"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /locus_tag="PLD53_08440"
     CDS             complement(<1..1940)
                     /locus_tag="PLD53_08440"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRC60982.1"
                     /translation="MTTMNQLSDSTMFFGGNAPFVEELYENYLDNPTAVPAEWRDYFD
                     RLAQMPGFVARDVAHAPVIAAFTELAKDGGFRPAATVSGGDIKKQSAVGQLVTAYRSM
                     GTRWAELDPLKRLPRPKIDELELGFYGFNDADLNQPFNVGSLKGLPETAKLGDIVETL
                     KQTYCGTVGVEYMYMTDYDEKRWLQERLESTRSRPSYTAEQKKRILERLTAAETLERY
                     LHTRYVGQKRFSLEGGDSLIVAMDETIRAGGELGIDEVVIGMAHRGRLNVLVNTLGKA
                     PSMLFAEFEGKKKSDLSAGDVKYHMGFSSDVSTPKGPCHLTLAFNPSHLEIVNPVVEG
                     SVYARQVRRGEGSKSKVLPVIIHGDSAVAGQGVNQEMLNFAQTRGYGTGGTLHIVVNN
                     QIGFTTSDPRDYRSGHYCTDIFKMADAPIFHVNGDDAEAVAMVTQLAVEFRQKFAKDV
                     VVDIVCFRKLGHNEQDEPMVTQPLMYKIISKHPGTRKLYGDKLIAEGVLPAEGPDQMI
                     QEYREHLDKGELLYNPVLAGYKHPNMIDWTPFLTKKYIETCDTTVPMKELQRLSQRLT
                     TFPEGFTLHSRVKKIAEDRAAMGEGKLPVDWGMAENLAYASLLVSGYGVRISGEDVGR
                     GTFFHRHAAFHDQNRESWDEGT"
     gene            complement(2047..3345)
                     /gene="gltA"
                     /locus_tag="PLD53_08445"
     CDS             complement(2047..3345)
                     /gene="gltA"
                     /locus_tag="PLD53_08445"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRC60983.1"
                     /translation="MTTERKATLTIDGQAPVDFQIMTPTHGNDCIDIRTLGAKTGLFT
                     YDSGFLSTASCKSKVTFIDGDVGQLLYRGYPIEQLAENCNFLEVTYLLKNGELPNAKQ
                     KIDFENTIKNHTMVHEQLSKFFSGFRRDAHPMAVMTGVVGALSAFYHDAMDFSDAEHR
                     NVSFNRLVAKLPTIVAMAYKYNTGAPFMYPDNSLDYTANFMRMMFGNPCEKYVPNPVL
                     VRALDTIFTLHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEACLQ
                     MLEEIGDVSRVPEFIARAKDKNDSFKLMGFGHRVYKNFDPRAMLMRKVCNEVLTELGL
                     ENDRLFKLAMELERIALEDPYFVEKKLYPNVDFYSGIVQKAIGIPTEMFTCIFALART
                     VGWMTQWEEMITDPEYKIGRPRQLYIGAAKRDVVPLAQRG"
     gene            complement(3429..3671)
                     /locus_tag="PLD53_08450"
     CDS             complement(3429..3671)
                     /locus_tag="PLD53_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HRC60984.1"
                     /translation="MLREDFEKLRWRCVRRGLLEVDISLTRFLDEQFEGLTDEEQLAF
                     VELADMEDYDVWHLISGQAECTDPRISSIVAMLRKS"
     gene            complement(3671..4381)
                     /locus_tag="PLD53_08455"
     CDS             complement(3671..4381)
                     /locus_tag="PLD53_08455"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HRC60985.1"
                     /translation="MSKRTVQFSIYRYDPDKDDAPYMQEISVDLEPTDRKLLDALTKL
                     KAKDDTIAYRRSCREGICGSDAMNINGKNGLACLTDIDELKQPIVLRPLPGLPVIRDV
                     IVDMTQFFKQYHSIKPYLINNDPPPERERLQSPEDREELNGLYECILCACCSTACPSF
                     WWNPDKFVGPAGLLAAYRFIADTRDQATNERLDNLEDPYRLFRCHTIMNCVDVCPKGL
                     NPTKAIGKIKDMMLRRAI"
     gene            complement(4394..>5635)
                     /locus_tag="PLD53_08460"
     CDS             complement(4394..>5635)
                     /locus_tag="PLD53_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRC60986.1"
                     /translation="AMEMETGQIVIFHARATIFATGGAGRIYYSSTNAFINTGDGLGM
                     AARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILRNSSKERFMERYAPNAKDL
                     ASRDVVSRAMATEIKEGRGCGVNKDYVLLDVTHLDPATIMKRLPGIREIGIQFAGVDC
                     IKEPLPVVPTCHYQMGGIPTHYSGRVVMPQGGDMSAVVPGFYAGGECACASVHGANRL
                     GTNSLLDLLVFGKSAGDSAVEDLKGGRAHRDLPKDAGDLALERISAIDNRKGGASVAE
                     TRLAMQRTMQDHAGVFRFGDMLKQGVTKILEVEKAARQMEIKDKSKVWNTARIEALET
                     ENLIEVAKATMISAEARKESRGAHVRDDAPDTAEFPNGRNDAEWLKHTLYFPQDNKIM
                     YKPVNMQPLTVEAIELKTRSY"
BASE COUNT         1280 a         1488 c         1580 g         1287 t
ORIGIN      
        1 taggtgcctt catcccagct ttcacgattt tggtcgtgga aggcggcatg acggtggaag
       61 aaggtgccgc gaccgacgtc ttcgccggag atgcggacgc cgtagccgga gacgagcagc
      121 gaagcgtaag cgaggttttc tgccataccc cagtcaaccg gcagcttgcc ttcgcccata
      181 gcggcgcgat cttcggcgat tttcttgact cgggagtgca gcgtgaagcc ttccgggaac
      241 gtggtgagac gctgggaaag acgttgcagc tccttcatcg gcaccgtggt atcgcaggtt
      301 tcgatgtatt ttttagtcag gaacggtgtc cagtcgatca tgttcgggtg cttgtagcca
      361 gccagcaccg ggttgtagag caactcaccc ttgtcgagat gttcgcggta ttcctggatc
      421 atctggtccg ggccttcggc cggcaggacg ccttcggcaa tgagtttgtc gccgtagagc
      481 ttgcgggtac cggggtgctt gctgatgatc ttgtacatca gcggctgggt gaccatcggt
      541 tcgtcttgtt cgttgtggcc gagtttgcgg aagcagacga tgtcgacgac aacgtccttg
      601 gcaaatttct gacggaactc aacagcaagc tgggtcacca tggcgacagc ttcagcgtcg
      661 tcaccgttga cgtggaagat cggggcatcg gccatcttga agatgtcggt acagtagtgg
      721 ccggagcggt agtcgcgcgg gtcgctggtg gtgaagccga tctggttgtt gacaacgatg
      781 tgcagggtgc caccggtgcc gtagccacgg gtctgggcga agttgagcat ttcctgattg
      841 acgccctggc cagcgacggc agagtcgccg tggatgatga ccgggaggac tttggatttg
      901 ctgccttcgc cgcgacggac ttggcgggcg tagacggagc cttcgacgac cgggttgacg
      961 atttcgaggt gggaaggatt gaaggccagg gtcaggtggc acgggccctt cggcgtcgag
     1021 acgtcggagg agaagcccat gtggtatttg acgtcacctg ccgagagatc gctcttcttc
     1081 ttgccttcga attcggcaaa caacatggac ggtgctttgc ccagggtgtt aacgaggacg
     1141 ttgaggcggc cacggtgggc cataccgatg acgacttcgt caatgccaag ctcaccgcca
     1201 gcacggatgg tttcatccat agccacaatc agggagtcgc cgccttcgag ggagaaacgc
     1261 ttctggccga cgtagcgggt gtgcaggtaa cgctcaaggg tttctgctgc ggtcaaccgt
     1321 tcaagaatgc gttttttctg ttcagcggta taggacggac gcgaacgcgt actttcgagg
     1381 cgctcttgca accagcgctt ttcgtcgtag tcggtcatgt acatgtactc gacacccacg
     1441 gtgccgcaat acgtctgctt gagggtttcg acgatatcgc caagcttggc agtttcgggc
     1501 aggcctttga gcgagccgac gttgaagggt tggttcaggt cagcatcgtt aaaaccgtag
     1561 aagccgagtt cgagttcgtc gattttcggc cggggcaggc gcttgagcgg gtcgagttcg
     1621 gcccagcggg tgcccatgga acgataggcg gtgacgagct ggccaacggc ggattgtttc
     1681 ttgatatcgc caccggagac ggtcgctgca gggcggaatc cgccgtcctt agccagttcg
     1741 gtaaaggcgg caataaccgg cgcgtgggcg acgtcgcggg cgacaaagcc cggcatctgt
     1801 gccaagcggt cgaagtagtc gcgccattca gcaggaacgg cggtcggatt gtcgagatag
     1861 ttttcgtaaa gctcttctac aaacggcgcg ttgccgccaa agaacatcgt gctatctgat
     1921 agttggttca tcgtggtcat aggcgtcccc tctagggtgt tgtctttttt gggtagctac
     1981 agttcacggc atcactaaac gccgcatgaa aaaaagggcg accgattggc cgcccgattt
     2041 tttaacttag ccgcgttggg cgagcggcac cacatcgcgc ttggcagcgc cgatgtagag
     2101 ctgacgcgga cggccgatct tgtattccgg atcggtgatc atttcttccc actgcgtcat
     2161 ccagccaacc gtacgtgcca aggcgaagat acaggtgaac atttcggtcg gaatgccaat
     2221 ggccttctga acgatacccg aatagaagtc gacgttcggg tagagtttct tctcgacgaa
     2281 atacggatct tccagagcga ttctttccag ctccatggcc agcttgaaca agcggtcatt
     2341 ttccagaccc agctcggtca gcacttcatt gcaaactttg cgcatcagca tggcgcgtgg
     2401 gtcgaagttc ttgtagacgc ggtgaccgaa gcccatcaat ttgaaggaat cgttcttgtc
     2461 cttggcgcga gcaataaatt cggggacgcg cgaaacatca ccgatttctt cgagcatctg
     2521 cagacaggcc tcgttagcgc cgccgtgagc cgggccccac aaacaggcaa taccggcagc
     2581 gatacaggca aacggattgg caccggagga gccagccagg cgtaccgtgg aggtcgatgc
     2641 attctgttcg tgatcagcgt gcaaggtgaa gatggtatca agggcgcgaa cgagcactgg
     2701 attcgggaca tacttttcgc acgggttacc aaacatcatg cgcatgaagt tggcggtgta
     2761 atccagcgag ttgtccggat acatgaaagg cgcaccggtg ttgtacttgt aggccatcgc
     2821 gacaatagtc ggcagcttgg cgaccaaacg attgaagctg acattgcgat gctcggcgtc
     2881 cgagaaatcc atggcatcgt ggtagaaggc agaaagcgca ccgaccacac ctgtcatcac
     2941 cgccatcggg tgagcatcgc gacggaaacc ggagaaaaac ttggaaagct gctcatgcac
     3001 catcgtgtga ttcttgatag tgttttcgaa atcgattttc tgcttggcat tgggcagttc
     3061 cccgttcttc agcagatagg taacttccag aaaattgcag ttttcagcca gttgctcaat
     3121 cgggtatcca cgataaagca gttggccgac atcgccatca atgaacgtta ctttggattt
     3181 gcagcttgcg gtggacagaa agccggagtc ataagtgaac aagccggttt tggcacccaa
     3241 ggtacggatg tcaatacagt cgttgccgtg cgtcggggtc atgatctgga aatcaaccgg
     3301 agcctgtccg tcgattgtca aagttgcttt gcgttcggtc gtcatggatt cttcccttta
     3361 caggtgttgg ttgttactac agtgtcaaaa cagcgatcaa tttgcctaat ttagaacgta
     3421 aagcttactt aactcttacg tagcatcgcc acaatcgagg aaatacgggg atcagtacat
     3481 tctgcctggc cactgattag atgccaaaca tcataatctt ccatatcagc aagctcaacg
     3541 aacgcaagct gctcttcgtc cgtcaggcct tcaaactgtt catcgagaaa tcgcgtcagc
     3601 gaaatatcga cttccagcaa gccccgacga acgcagcgcc agcgtagttt ttcaaaatcc
     3661 tcgcgaagca ttaaatcgct ctacgaagca tcatgtcctt tattttgccg atcgccttgg
     3721 tcggattcaa tcctttaggg caaacgtcaa cgcagttcat gatggtatgg caacggaaga
     3781 gtcggtacgg atcttcaaga ttatccaaac gttcgttggt ggcctggtcg cgggtatcgg
     3841 caataaaccg ataagcagcc aacaatcctg ccggcccaac aaatttatca ggattccacc
     3901 agaacgacgg gcaagctgtc gaacagcagg cacacagaat gcactcatac aagccattca
     3961 actcttcacg atcttctggc gactgaaggc gctcgcgctc cggtggcgga tcgttgttga
     4021 tcaaataggg tttgatcgag tgatactgtt tgaagaactg ggtcatatcg acgatcacat
     4081 cgcgaatcac cggcaaaccg ggaagcggac gaagcacgat gggctgtttg agctcatcga
     4141 tatcagtcaa acaggccaaa ccattcttac cgttgatatt catggcatca gaaccacaaa
     4201 taccttcacg gcaggatcgg cgataggcaa tagtgtcgtc ctttgccttt agcttagtca
     4261 gcgcgtccag taatttgcgg tcagtcggtt cgagatcaac cgaaatttcc tgcatgtaag
     4321 gcgcatcgtc cttgtccgga tcgtagcggt agatgctgaa ttgaaccgtg cgtttgctca
     4381 ttctgatctc caattagtac gagcgcgtct tgagctcgat agcctcgacg gtcaagggtt
     4441 gcatattgac cggcttgtac atgatcttgt tatcctgcgg gaaatacagg gtgtgcttga
     4501 gccactcggc atcattccga ccattcggaa actcggccgt atccggcgca tcatctcgga
     4561 cgtgggcgcc acgggactcc ttgcgggctt cggcagagat catggttgcc ttggcaactt
     4621 caatcaggtt ctctgtctca agggcttcaa tacgagcggt attccagacc ttggatttat
     4681 ccttgatctc catttgacgc gcagccttct caacttcgag aatcttggtg accccctgtt
     4741 tgagcatgtc gccaaaacgg aaaacccctg cgtgatcctg catggtgcgt tgcatcgcca
     4801 aacgcgtctc ggcaacgctg gcgccgcctt tgcgattgtc gattgcactg atacgttcca
     4861 gagccagatc tcctgcatcc ttcggtagat cacgatgagc gcgtccgcct ttcaaatcct
     4921 caaccgcgga atcaccggcc gatttaccaa aaaccagcag gtctagcaga gaatttgtac
     4981 ctaggcgatt ggcaccatgt acagaagcgc aggcacactc accaccggca tagaagccag
     5041 gaacaacggc agacatgtca ccaccttgcg gcatcacaac acgacccgaa taatgcgtag
     5101 gaataccacc catctggtaa tgacaggtcg gaaccaccgg caacggctct ttaatgcagt
     5161 cgacaccagc aaactggata ccaatttcac gaatacccgg caaacgcttc atgatcgtcg
     5221 caggatcaag gtgtgttacg tcgagtagca cataatcctt attcacgcca cagccacgac
     5281 cttccttaat ttcagtcgcc atggcgcggg aaaccacgtc acgggaagcc agatccttgg
     5341 cgtttggtgc atagcgctcc ataaagcgct ctttactgct gttacgcaga atgccacctt
     5401 caccacgcac gccttcggta atcagtacgc ccgcaccagc aacaccggtc gggtggaatt
     5461 gccagaattc catgtcctcg agaggaatgc cagcacgcgc tgccataccc aaaccatcgc
     5521 cagtattgat aaaggcattg gtagaagaat aataaatacg accagcgccg ccagtagcaa
     5581 aaatggtggc acgagcgtgg aagatcacga tctggccggt ttccatttcc atggc
//
LOCUS       DAORPM010000163         5573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4036_length_5573_cov_19.768213, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000163 DAORPM010000000
VERSION     DAORPM010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4036_length_5573_cov_19.768213"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1650)
                     /locus_tag="PLD53_08465"
     CDS             complement(184..1650)
                     /locus_tag="PLD53_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRC60987.1"
                     /translation="MGKPTGFMEFQHLSEAYEATDQRLKHYREFIITLSDAHASVEGA
                     RCMDCGIPFCNNGCPVNNIIPDWNDLVYRGNWKQALDVLHSTNNFPDFTGRICPAPCE
                     AACTLNINNDPVGIKSIEHAIIDKGWEMGWIVPQPAQAKTGKRVAIVGSGPAGLAAAQ
                     QLARVGHDVTVFEKNDRIGGLLTYGIPDFKLEKLHIERRIEQMEAEGVSFRTGVMIAC
                     KDLPAGIVSDAGEFISADQLTSDFDAVILAAGAEVPRELPIPGRDLNGIYPALEFLIP
                     QNREVSGGKANPISAKDKHVVVIGGGDTGSDCVGTSYRHGAASVTQFELMPQPPEQEN
                     KALTWPYWPLKLRTSSSHLEGNTQRKFAIATKEFVGEEGVLKSLKTVELDWKDGKMNE
                     ISGSEKTVQADLVFLAMGFLSPVGGLLDAFGVDKDARGNAKATTDGEGCYATNATKVF
                     AAGDTRRGQSLVVWAIREGRQCAREVDIYLMGESTLPR"
     gene            complement(1651..>5573)
                     /locus_tag="PLD53_08470"
     CDS             complement(1651..>5573)
                     /locus_tag="PLD53_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRC60988.1"
                     /translation="GEINTLRGNYNWMRAREKGTSSPLMGADLEKIWPVIYPNQSDSA
                     SFDNALELLVMGGYSLAHAMMLLIPEAWESHTLMDEKRRAFYEYHAAMMEPWDGPAAV
                     AFTDGCQIGATLDRNGLRPARYLVTDDDLVVMSSEAGVLPIPEENIIKKWRLQPGKMF
                     LIDLDQGRIIDDLELKETLSRQKPYRDWVARINVKLSGLDLGMHVTAPEYSVPLLKRQ
                     QAFGFSQEDIKFILEPLARTGEEATGSMGNDTPLAVLSNKDKSLFMYFRQLFAQVTNP
                     PIDSIREQLVMSLVSFIGPKPNLLGINEINPPYRLEVSQPVLNFADMVRLRNIAACTE
                     DKFLSAELDICYPLAWGNEGVEARIASLCAEAEDAVHRGCGILIVSDRHMNQDNVAIP
                     ALLATSAVHQHLVTQGLRTLVGLVVETGSVRETHHFAVLAAYGAEAVHPYLVLETLQQ
                     LAGESEAEREKAIKHFVKGIGKGLLKVMSKMGISTYMSYTGAQIFEAIGLQRKMVDKY
                     FTGTETQIEGIGIFEVMEEAIRQHRHAFSTDPVLTNMLDAGGEYAFRIRGEEHMWTPD
                     AIARLQHSTRTGQYESYKEYATIINDQTKRHMTLRGLFEIKPAGPAVPLEEVEPAKEI
                     VKRFATGAMSLGSISTEAHSTLAIAMNRIGGKSNTGEGGEDPARFKPVKAGQMVSEII
                     GKGRIERDLQLKEGDSLRSAIKQVASGRFGVTVEYLSNADQIQIKMAQGAKPGEGGQL
                     PGHKVSEYIGFLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNANSQASISVKLVS
                     EIGVGTVAAGVTKAKADHLVIAGHDGGTGASPLSSIKYAGSPWELGLAETQQTLVLNR
                     LRGRVRVQADGQMKTGRDVLIGALLGADEFGFSTAPLVVEGCIMMRKCHLNTCPVGVA
                     TQDPVLRRKFSGQPEHVVNYFFFVAEELRELMAQMGVRNFNDLIGRADLLDMRQGIDH
                     WKARGLDCSRIFHCPDRQPDEPVYHCEEQDHGLDKALDKQLIELARPAIEEGNQVNIE
                     LPIRNVNRTVGAMLSGRIARKYGHAGLPDSTIQITLNGTAGQSFGAFLARGVTMDLIG
                     EGNDYAGKGLSGGRIIVRPHGGFRGDTTSNIIIGNTVLYGAIAGEAFFAGVAGERFAV
                     RNSGATAVVEGVGDHGCEYMTGGTVVVLGITGRNFAAGMSGGVAYVLDEDGSFESRCN
                     MAQVALEPVEEEIAARKGSGAGDDLESHGKVDVRHLGMEDEILLKGLVEQHARRTGSV
                     RAGKILEDWATYRARFVKVMPHEYRRALTEMAAQKAGNQQREAA"
BASE COUNT         1051 a         1699 c         1598 g         1225 t
ORIGIN      
        1 gtgttggttg gcggggtgac ctgcaaggat ttctgcaatt ccgggttcat tgacttctcc
       61 tgcagtctga gcagccgcta ctatgccata gccttgccca gtgcaaaata aaaaccccct
      121 tccgttagcc taaagggggt ttttacagga gaggtcgatg cccttctcaa cctggattgc
      181 agatcaacgt ggaagcgtac tctcccccat caggtagatg tcaacctccc tggcgcactg
      241 gcggccttcg cggattgccc agacaaccag cgactgtccg cggcgtgtat cgccagcggc
      301 aaacaccttg gtggcattcg tcgcgtaaca gccttcgccg tcggtggtcg ccttggcatt
      361 tccgcgcgca tccttgtcga cgccgaaggc gtcgagcagt ccgccgactg ggctcaggaa
      421 gcccatggcc agaaagacca gatcagcctg aacagttttt tcgctgccgg aaatttcgtt
      481 catcttcccg tctttccaat ccagctcgac tgttttcagc gatttgagaa caccctcttc
      541 gccgacaaac tccttggtcg cgatggcaaa cttgcgttgc gtattgcctt ccagatgaga
      601 agacgaggtt cgcagtttca gcggccaata cggccaagtc agcgccttat tctcctgttc
      661 gggcggttgc ggcatcagct cgaattgcgt caccgatgca gccccgtggc gatagctcgt
      721 ccctacgcag tcggaaccgg tatccccgcc accgataacg acaacatgct tgtccttggc
      781 gctgatcgga ttggctttgc cgccactcac ttccctgttc tgcggaatca ggaactctag
      841 tgccggatag atgccgttca gatcgcgccc cggaatgggc agctcgcgtg gaacttcggc
      901 accggccgca agtatcacgg cgtcaaaatc gctggtcagt tggtcagccg agatgaattc
      961 gccggcatca ctcacgatgc ctgcgggcag atccttgcaa gcgatcatta cgccggtacg
     1021 gaagctgacg ccctcggctt ccatctgttc gatgcgtcgt tcaatatgca atttctcgag
     1081 cttgaagtcc ggaatgccgt aggtcagcaa cccgccgata cgatcgttct tctcgaatac
     1141 cgttacatcg tggccgacac gtgcaagttg ctgggccgcc gcaagaccag cggggccgga
     1201 accgacgatg gcgacgcgct tgcccgtttt cgcctgcgcc ggctgaggaa cgatccagcc
     1261 catctcccag cccttgtcga taatcgcatg ttcgattgac ttgataccca ccgggtcgtt
     1321 gttgatgttg agcgtacagg cggcttcgca gggtgccgga caaatccgcc cggtgaagtc
     1381 cgggaagttg ttggttgaat gcagcacgtc cagcgcctgt ttccagttgc cgcgatagac
     1441 caggtcattc cagtccggaa tgatgttgtt gaccgggcac ccgttgttgc agaaaggaat
     1501 gccgcaatcc atgcagcgcg cgccttccac gctggcgtgt gcatcgctca gtgtgatgat
     1561 gaactcgcgg taatgcttca accgttgatc ggtggcctca taggcctccg acaggtgctg
     1621 aaactccata aagccagtag gcttacccat ttatgcggcc tcccgttgct gatttccggc
     1681 tttctgcgcg gccatttctg ttagcgcccg cctgtattca tgcggcatca ccttgacgaa
     1741 gcgtgcgcgg tatgtcgccc agtcttcgag gatctttccc gctcgcacac tgcctgtgcg
     1801 tcgggcgtgt tgctcgacca gccccttgag cagaatctcg tcttccatgc caaggtgacg
     1861 gacatcaacc ttgccgtggc tttcaagatc gtcgccggcc ccgctcccct tgcgcgcggc
     1921 gatttcttcc tccacgggtt ccagtgccac ctgcgccatg ttgcagcgcg actcgaagct
     1981 gccgtcctca tccagaacat aggccactcc ccccgacatg ccggcggcga agttccgccc
     2041 ggtgatgccg agcacgacaa cggtaccgcc tgtcatgtac tcgcacccgt gatcgccaac
     2101 gccctcgacc acggccgtag cgcccgagtt gcgaaccgca aagcgctctc ccgcgacacc
     2161 ggcgaaaaac gcctcaccgg caatcgcgcc atacagcacg gtattgccga tgatgatgtt
     2221 ggaggtcgta tcgccgcgaa atccgccatg tggacgaaca atgatgcgcc cgcccgatag
     2281 ccccttgccg gcgtagtcgt tgccctcgcc gatcagatcc atcgtaacgc cgcgcgccag
     2341 aaacgcgcca aaactctggc ctgccgtgcc gttcagggtt atttgaatcg tgctgtcagg
     2401 caatcccgca tggccgtatt ttctggcgat ccgtccggaa agcatggcgc cgacagtgcg
     2461 attgacattg cggatcggca gctcgatatt gacctgattg ccttcttcga tagccggtct
     2521 ggccagctcg atgagttgct tgtcgagcgc tttgtcgaga ccatgatcct gttcttcgca
     2581 gtgataaacc ggttcgtcag gctgtctgtc ggggcaatgg aatatacggc tacagtcgag
     2641 acctcttgcc ttccagtgat cgataccctg acgcatgtca agcaaatcgg cgcgcccgat
     2701 caggtcgttg aagttgcgga cacccatctg tgccatcaat tcgcgcagtt cttcggcgac
     2761 gaagaagaaa tagttgacga catgctccgg ctgaccggag aactttctgc gcaaaaccgg
     2821 atcctgtgtt gccacgccca ccgggcatgt gttgaggtgg cacttgcgca tcatgatgca
     2881 gccctcgacg accagaggcg ccgtggaaaa gccgaattcg tcggcgccaa gcagggcgcc
     2941 gatcaataca tcgcgcccgg tcttcatctg gccgtcggcc tgaacgcgta cgcggccgcg
     3001 cagacggttg agtaccagcg tctgctgtgt ctcggcaagg cccagttccc acggcgaacc
     3061 ggcgtacttg atcgatgaaa gggggctggc gccggttcca ccgtcgtgac cggcgatcac
     3121 cagatgatcg gccttggcct tggtgacacc cgcggcaacc gtgccaacgc cgatctcaga
     3181 caccagcttg acgctgatcg acgcctgcga attggcgttc ttcaggtcgt gtatgagctg
     3241 cgccaggtct tcgatggagt agatatcgtg gtgcggtggt ggcgaaatga gtccgacgcc
     3301 cggtaccgag tacctgagga agccgatata ttcggaaacc ttgtgccccg gaagctggcc
     3361 gccttcgccc ggcttggcgc cttgcgccat cttgatctgg atttgatcgg cattggacag
     3421 atactccacc gttacgccga aacggccgga ggcgacttgc ttgattgccg aacgcagact
     3481 gtcaccttcc ttaagttgca gatcgcgctc gatgcggccc ttgccgatga tttcggagac
     3541 catctgaccg gctttgaccg gtttgaatcg cgcagggtct tccccgccct cgccggtgtt
     3601 cgacttgccg ccgatccggt tcatggcgat ggccagggtg ctgtgcgcct cggttgaaat
     3661 cgaaccgagc gacatggctc cggtggcgaa tcgcttgacg atctccttgg ccggctcgac
     3721 ctcttccaat gggaccgccg gtccggccgg cttgatctcg aacagcccgc gcagtgtcat
     3781 gtgccgcttc gtctggtcgt tgatgatggt ggcgtattcc ttgtagcttt cgtactgccc
     3841 ggtgcgcgtt gagtgctgga gcctggcaat ggcatcgggc gtccacatgt gctcttcacc
     3901 acgaatgcgg aaagcgtatt cgccaccggc gtccagcatg ttggtcagca ccggatcggt
     3961 gctgaaggcg tgccggtgtt gccgaatggc ctcctccatc acctcgaaaa tgccgatccc
     4021 ctcgatctgc gtctcggtgc cggtaaagta cttgtcaacc atcttccttt gcaggccgat
     4081 ggcctcgaag atctgggcgc cggtatagga catgtaggtc gatatgccca tcttggacat
     4141 gaccttcagc aatcccttgc cgatgccttt gacgaagtgc ttgatggcct tttcgcgctc
     4201 cgcttcgctt tcacccgcca gttgctgcaa ggtttcgagt accaggtagg gatgcacggc
     4261 ttcggcgcca taagcggcca gaacggcgaa gtgatgcgtc tcacgaaccg agcctgtctc
     4321 gacaacgagg ccgaccagag tgcgcaaacc ctgggttacc agatgctggt gcaccgccga
     4381 agtggcgagc agggccggga tggccacgtt atcctggttc atatggcgat ccgaaacgat
     4441 caggattccg cagccacgat gcacggcatc ctccgcctcg gcgcacaatg aagcgatacg
     4501 tgcctcaacg ccttcgttgc cccatgccag cgggtagcag atatcgagct cggcagacag
     4561 aaacttgtcc tccgtgcatg cggcgatgtt gcgcaacctg accatgtcgg caaagttcag
     4621 caccggctgc gacacttcaa gccggtaggg cgggttgatt tcgttgatgc caagcaggtt
     4681 gggcttgggg ccgatgaagg agaccagcga catgaccagt tgctcgcgaa tcgagtcaat
     4741 gggcgggttg gtcacctgcg caaacagttg cctgaaatac atgaacagcg atttgtcctt
     4801 gttcgagagt accgccagcg gcgtgtcgtt acccatcgag ccagtggcct cctcgccagt
     4861 cctggccagc ggctccagaa tgaactttat atcttcctgg ctgaagccga atgcctgctg
     4921 gcgcttgagc aagggcaccg agtactccgg cgcagtcaca tgcataccga ggtccaggcc
     4981 gctgagcttg acgttgatgc gtgccaccca gtcccgatag ggtttctgcc gcgacagtgt
     5041 ttccttgagc tcgaggtcgt caatgatgcg cccctgatcg agatcgatga ggaacatttt
     5101 gcccggttgc agacgccact tcttgatgat gttttcttcg ggaatcggca acaccccggc
     5161 ttccgatgac atgacaacga gatcgtcgtc ggtaaccaga tagcgtgccg ggcgcaggcc
     5221 attgcggtca agcgttgcgc caatctgaca gccatcggta aaggccacgg cggccggacc
     5281 gtcccagggc tccatcatgg ccgcatgata ttcatagaag gcgcgacgtt tttcgtccat
     5341 caaggtgtgt gattcccatg cctccggaat cagcagcatc atggcgtgcg ccagtgagta
     5401 accgcccatc accagcaact ccagcgcgtt gtcgaacgac gccgaatcgg actggttggg
     5461 atagatcacc ggccagattt tttccagatc ggcacccatc agcggcgagg aagtgccttt
     5521 ttcgcgtgcg cgcatccagt tgtagttgcc gcgcagggtg ttgatttcgc cgt
//
LOCUS       DAORPM010000164         5557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4054_length_5557_cov_20.384224, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000164 DAORPM010000000
VERSION     DAORPM010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4054_length_5557_cov_20.384224"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3077
                     /gene="carB"
                     /locus_tag="PLD53_08475"
     CDS             <1..3077
                     /gene="carB"
                     /locus_tag="PLD53_08475"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRC60989.1"
                     /translation="ILVNSNPATIMTDPEMADVTYIEPITWNVLEKIIEKERPDALLP
                     TMGGQTALNCALDLARHGVLARFSVEMIGASKDAIDKAEDREKFKNAMTRIGLGSARS
                     AMAHSMEEAQQVQAALGFPTIIRPSFTMGGSGGGIAYNQEEFVEICKRGLEASPTNEL
                     LIEESLIGWKEFEMEVVRDKNDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQ
                     IMRDASVAVLREIGVDTGGSNVQFSICPEDGRMIVIEMNPRVSRSSALASKATGFPIA
                     KVAAKLAVGYTLDELANEITGGRTPVSFEPSIDYVVTKVPRFAFEKFPQADSHLTTQM
                     KSVGEVMAIGRTFQESFQKALRGLEVGVDGMNQRTTDREELERELGEPGPERIWYVGD
                     AFENGFTLDEVHTLTHIDPWFLAQIEDIVRIEMKLDDMAFEEIDAGALRELKRKGFSD
                     RRLAKLLKSTEALVRARRHSLGIRPVYKRVDTCAAEFSTSTAYMYSTYEEECESKPSD
                     RKKIMVLGGGPNRIGQGIEFDYCCVHAALALREDGYETIMVNCNPETVSTDYDTSDRL
                     YFEPLTLEDVLEIVAIEKPVGVIVQFGGQTPLKLARDLEANGVPIIGTSPDMIDAAED
                     RERFQKLLQDLGLKQPPNRTARTQPDALRLAAEIGYPLVVRPSYVLGGRAMEIVHEPR
                     DLERYMREAVKVSNDSPVLLDRFLNDAVEVDVDALSDGETVIIGGVMEHIEQAGVHSG
                     DSACSLPPYSLSAEVQDELRRQTSLMARALNVCGLMNVQFAIQDDVVYVLEVNPRASR
                     TVPFVSKATGLQLAKIAARCMAGQSLARQGVTREIVPPYYSVKEAVFPFNKFPGVDTI
                     LGPEMKSTGEVMGVGKTFEEAFVKSQMAASVKLPASGKVFISVKESDKSKVAEIARDL
                     HSAGFAILATRGTASTIASAGIPVTVVNKVTEGRPHVVDMIKNNEISLIINTVDEKRQ
                     AVSDSRSIRTTGLAARVTMYTTIWGAEAAAVGIKSCGELVVYPIQSLHEQLG"
     gene            3225..3701
                     /gene="greA"
                     /locus_tag="PLD53_08480"
     CDS             3225..3701
                     /gene="greA"
                     /locus_tag="PLD53_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRC60990.1"
                     /translation="MSKVPLTVTGAEKLRAELHQLKTVERPSVIAAIAEARSHGDLSE
                     NAEYDAAKERQGFVEGRIKEVESKLSNAQIIDPKLLDADGRCVFGATLDLEDQDSGLP
                     VTYQIVGEDEADIKDGKISVNSPIARALIGKYAGDIAQVQAPGGLREYEVLDVRYE"
     gene            3758..4219
                     /locus_tag="PLD53_08485"
     CDS             3758..4219
                     /locus_tag="PLD53_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HRC60991.1"
                     /translation="MRRIPEALFFVALTLWVGGLWAIGYLVAPTLFATLADRHLAGMI
                     AGKLFTLIGWVGLGCGAYLLSFMLFSQGSTVLKRRAFWVVLVMLLLTAVGQFGIHPLL
                     AQLKADALPREVMDSVLRDRFVAWHGISSILYLLQSLLGLMVVVCAGRGAR"
     gene            complement(4240..4596)
                     /locus_tag="PLD53_08490"
     CDS             complement(4240..4596)
                     /locus_tag="PLD53_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HRC60992.1"
                     /translation="MLDITADQRRELRARAHALNPVVSISQNGLSESVMQEISTSLDS
                     HELIKIRVFSDDRDVREQYLASICEQLGAAPVQHIGKLLVVWRPLPAPRVPPRLRRSP
                     PVPRRSKRSFQGGSRG"
     gene            4640..5260
                     /locus_tag="PLD53_08495"
     CDS             4640..5260
                     /locus_tag="PLD53_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRC60993.1"
                     /translation="MKRTRTSNAWLREHVNDHYVQRAKAEGYRSRASFKLMEIDDRDR
                     LFRPDEIVVDLGAAPGGWSQVAVQRMKGRGRVIALDLLEMDPVGGVDFIQGDFRDEDV
                     LQKLETTLAGVSVGLVLSDMAPNMSGILVTDQARVMHLAELGLEFSRNWLKPEGVFLV
                     KVFHGYGYEDFVREMRAAFRTVSSRKPGASRDRSTEVYLLGRGLKT"
     gene            5407..>5557
                     /locus_tag="PLD53_08500"
     CDS             5407..>5557
                     /locus_tag="PLD53_08500"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRC60994.1"
                     /translation="MAVWLVIGLVLMTVFNQFNSRQVAQASMEYSQFMDEVQRGSISK
                     VVIEGR"
BASE COUNT         1154 a         1424 c         1757 g         1222 t
ORIGIN      
        1 tgatcctggt caattcgaac ccggcgacga tcatgaccga tccggaaatg gccgatgtga
       61 cctacatcga gccgatcacc tggaacgttc tcgagaagat catcgaaaag gagcgccccg
      121 acgctctgct gccgaccatg ggcggccaga ccgcgctcaa ctgtgcgctc gatctggcta
      181 ggcacggcgt actggccaga ttcagcgtcg agatgatcgg cgcatcgaag gatgccatcg
      241 acaaggccga ggaccgcgaa aaattcaaga acgcgatgac caggatcggc ctcggttcgg
      301 cacgttcggc aatggcgcac tcgatggagg aagcgcagca ggtacaggct gcgctgggtt
      361 tcccgaccat tatccgtccg tcctttacga tgggcgggtc gggtggaggc attgcctaca
      421 accaggagga gttcgtcgaa atctgcaagc gcgggctcga agccagcccg accaacgagc
      481 ttttgatcga ggagtcgttg atcggctgga aggagttcga gatggaggtg gtccgcgaca
      541 aaaacgacaa ctgcatcatc gtctgctcga tcgagaacct tgatccgatg ggtgtgcata
      601 ccggcgattc gatcaccgtt gcgccggcgc aaacactgac cgacaaggaa taccagatca
      661 tgcgcgacgc ctcggtggcc gtcctgcgcg agatcggggt ggatactggc ggatcaaacg
      721 tgcagttttc gatctgtcct gaagacggcc gcatgatcgt catcgaaatg aacccgcgtg
      781 tttcgcgttc ttcggcgctg gcgtcgaaag cgaccggctt tccgatcgcc aaggtggcgg
      841 ccaagctggc cgtggggtac acgctcgatg aactggccaa cgaaatcacg ggtggcagga
      901 cgccggtttc tttcgagccg tcgattgact acgtggtgac caaggtgccg cgctttgcct
      961 ttgagaagtt tcctcaggcc gattcgcatc tcaccacaca gatgaagtcg gtcggcgaag
     1021 tgatggccat cggtcgcacg ttccaggaat ccttccagaa ggcgctgcgc ggccttgaag
     1081 tgggcgtcga tgggatgaat caacggacaa ccgaccgcga ggaacttgag cgtgaactcg
     1141 gcgagcccgg cccggagcgc atctggtatg tcggcgatgc tttcgagaac gggtttacgc
     1201 tggatgaggt tcacaccctg acgcacatcg atccctggtt tctggcgcag atcgaggata
     1261 tcgtcaggat cgagatgaag ctcgatgata tggcgtttga agagatagat gccggggctt
     1321 tgcgtgaact caagcgcaag ggattttcag atcggcgtct ggccaagctg ctcaagtcaa
     1381 ccgaagcgct ggttcgcgcc cgtcgtcatt cgctgggcat tcgtcctgtc tataagcgtg
     1441 ttgatacctg cgcggcagaa ttctcgacca gtaccgccta tatgtactcg acctacgagg
     1501 aagagtgcga gtcgaagccg agcgacagaa agaagatcat ggtgctcggc ggtggtccga
     1561 accgcatcgg gcaggggatc gagttcgact actgttgcgt gcatgcggca ctcgcgttgc
     1621 gcgaagacgg ctacgaaacg atcatggtca attgcaatcc ggagacggtg tcaaccgact
     1681 acgatacctc ggatcgcctc tatttcgagc cgttgactct cgaagacgtg ctcgaaatcg
     1741 ttgccattga aaagccggtt ggcgtcatcg tgcagttcgg cgggcagacg ccgctcaagc
     1801 tggcgcgaga ccttgaagcg aacggggtgc ccatcatcgg caccagtccg gacatgattg
     1861 atgcggccga ggatcgggag cgctttcaga agctgcttca ggatcttggc ctcaagcaac
     1921 ccccgaatcg cacggcgcgt acccagcccg atgcgttgag gctggctgcc gaaattggct
     1981 acccgctggt cgttcggcca tcttacgtac ttggtggccg ggcaatggaa atcgtgcacg
     2041 agccacgcga ccttgagcgc tacatgcgcg aagcggtcaa ggtatcgaat gattcgccgg
     2101 tgctgcttga tcgatttctc aacgacgcgg tcgaagttga cgtcgatgcc ctgtccgacg
     2161 gcgagacggt catcatcggc ggtgtgatgg agcatatcga acaggccggg gtgcattccg
     2221 gcgactcggc ctgctcgcta cccccctatt cgctgtcggc ggaagtgcag gacgaacttc
     2281 gtcggcagac cagtttgatg gccagggcac ttaatgtctg cggtctgatg aacgttcagt
     2341 ttgccatcca ggacgatgtg gtctacgttc tggaagtgaa tccgcgtgcg tcacgcacag
     2401 tgcctttcgt ttccaaggct acggggcttc agttggcgaa gatcgccgcg cgctgcatgg
     2461 ccggtcagtc gctggcccgt cagggggtca cccgggaaat cgttccgccc tactactcgg
     2521 tcaaagaggc tgtgtttccc ttcaacaagt ttcccggtgt ggataccatt ctcggccccg
     2581 agatgaaatc gaccggtgaa gtgatgggtg tcgggaagac cttcgaggaa gcattcgtaa
     2641 agagccagat ggcggcaagt gttaaacttc ccgcgtccgg caaggtgttc atcagcgtca
     2701 aggagtcgga caagagcaaa gttgcggaaa tcgcccggga cttgcactcc gctgggtttg
     2761 ccatcctggc cacgcgtgga actgcatcga cgatcgctag tgccgggatt cccgttaccg
     2821 tggtcaacaa ggtgaccgaa ggacggccgc atgttgtcga tatgatcaag aacaatgaga
     2881 tttcgttgat catcaacaca gtggacgaga aacgtcaggc ggtcagcgat tcacgctcca
     2941 ttcgcacaac cggactggca gcacgcgtta cgatgtacac cacgatatgg ggcgctgagg
     3001 cagcggccgt aggcatcaaa agctgtggtg agctagtcgt ctacccgatc cagtcgttgc
     3061 acgaacaact gggttgacgg gagtggggtg gggtttgctt cgggccttgc gccattcggt
     3121 tttgtgattc aggcgcgtct tgcgcggaaa tgataaatga ttgttgaacc gcccgtgttg
     3181 cctatgatca ggtcgcaggc ggttattctc aggtagtaaa cgaaatgagc aaagttccct
     3241 taactgttac gggcgcggaa aaactgcgtg ccgaactgca tcaactgaaa acagtcgaga
     3301 ggccaagcgt gattgcggcg atcgccgagg cgcgctcgca cggtgatctt tcggagaatg
     3361 ccgaatacga tgccgccaag gaacgtcaag gtttcgtcga aggtcgtatc aaggaggtcg
     3421 agagcaaact gtccaatgca cagatcatcg accccaagct gcttgatgcc gatgggcgct
     3481 gtgtcttcgg cgcgacgctt gaccttgagg atcaggattc cggattgccg gtaacctatc
     3541 agatcgttgg tgaagacgag gccgatatca aggacggaaa gatttcagtc aattccccga
     3601 tagcgcgcgc actgatcggg aaatatgctg gagacattgc gcaggttcag gcgccgggtg
     3661 gtttgcgcga atacgaagtg ctcgatgtgc gttacgaata accggctgat tggtgattcg
     3721 ctttcttaac gcttgttgtt agttccaggg cctgaccgtg cgacgcattc cagaagcgct
     3781 tttctttgtg gcactgacct tgtgggtcgg cggtttgtgg gcgattggtt atctcgtcgc
     3841 accgactctt tttgcgacgc tggccgatcg gcatttggcg ggcatgattg ccggcaagtt
     3901 gttcacgctg atcggctggg tgggcctggg ttgcggggcc tatctgctgt ccttcatgct
     3961 gttcagccaa ggcagtaccg tcctgaaacg acgggccttc tgggtcgttc tggtgatgct
     4021 gctgctgact gcggttggcc agttcggaat tcatccattg cttgcgcaat tgaaggccga
     4081 tgcattgccg cgcgaggtga tggacagtgt cttgcgtgat cggttcgtcg cctggcatgg
     4141 catttcgagc attctctacc tgctgcagag tctgctgggc ttgatggtcg tggtgtgtgc
     4201 ggggcgtggg gcaaggtagg cgaaatccgg cggtcagatc tagccgcgtg atccgccctg
     4261 aaagctgcgc ttgctgcggc gcggcactgg agggcttcgg cgcagtcgag gtggtactct
     4321 aggcgcgggt agcggacgcc agacaacaag caatttgccg atgtgttgta ctggtgcggc
     4381 cccaagttgc tcgcagatgc tggccagata ctgttcgcgc acatcccgat cgtcgctgaa
     4441 gacacggatt ttgatcaatt catggctgtc aaggctggtg ctgatctctt gcatgaccga
     4501 ttcggacagc ccgttctggc tgatggaaac gacggggttg agagcgtggg cacgggcacg
     4561 cagctcacgt cgctggtcgg cagttatatc aagcattcag taaccttgcg caaagggcgc
     4621 attctaccga gtttaggcaa tgaaacgaac cagaacgagt aatgcgtggt tgcgcgaaca
     4681 cgtgaatgat cactacgttc aacgcgccaa ggccgagggt tatcgatccc gtgcttcgtt
     4741 caaactcatg gagattgatg accgggaccg tttgtttcgt cccgatgaaa tcgtcgttga
     4801 cctcggtgcg gctcccggcg gctggtcgca ggttgccgtt cagcggatga aaggcagggg
     4861 ccgggtcatc gcgctggatt tgctggaaat ggatcctgtg ggcggcgttg acttcattca
     4921 gggcgatttt cgtgatgaag atgtgctgca gaagcttgaa acgacattgg ccggcgtgag
     4981 tgtggggctt gtgctttccg atatggcccc caatatgtca ggtatcctcg ttaccgatca
     5041 ggcgcgggtg atgcatctgg cagagttggg gctggagttc tcaaggaact ggttgaaacc
     5101 agaaggcgta ttcttggtca aagtctttca cggctacggt tatgaagatt ttgtgcggga
     5161 aatgagagcg gccttcagga ccgtttcgtc gcgcaagccg ggcgcgtcgc gtgatcgcag
     5221 taccgaggtg tatttgctcg gtagaggatt gaaaacctga tgtttcctcg gaagcactcc
     5281 cgggattgca gcgcataaat cgctggagaa ggtttagaat gcagtattcg gtaatgggca
     5341 tcccttcagg agtttcctga ggagcaatga ggaagaggta cagcattgaa taatatgttc
     5401 aagaatatgg cggtgtggct ggtgatcggt ctggtgctaa tgaccgtctt caaccagttc
     5461 aacagccgcc aggttgcgca ggcgtccatg gagtattcgc aattcatgga cgaagtacag
     5521 cgcggctcaa taagcaaggt cgtgatcgaa ggacgtg
//
LOCUS       DAORPM010000165         5509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4112_length_5509_cov_13.408691, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000165 DAORPM010000000
VERSION     DAORPM010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4112_length_5509_cov_13.408691"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLD53_08505"
     CDS             complement(<1..224)
                     /locus_tag="PLD53_08505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC60995.1"
                     /translation="MNPFFHRFLMFAVGLILSTAAAAAPSVEIQTSMGKVVIELDSEK
                     APVTVKNFLQYAKDGFYNGTIFHRVMDGFM"
     gene            complement(224..1465)
                     /locus_tag="PLD53_08510"
     CDS             complement(224..1465)
                     /locus_tag="PLD53_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRC60996.1"
                     /translation="MAYSAGTRRLMRRAMLAITLIAACAAGTAASVDGNPHPAATFSD
                     SGPEPQLSRILVEIEKNRLDTALEETEALLKRYPNFRLAHLIKGDLLLARSAPLATFG
                     NASNAPKEQLADLRDEVVARLKAYRNRTNTSNYVPRYLLQMPPDQKYAIVVDTQKSRL
                     YLYKNDTDAGGRPRFVADYYITHGKLGSDKRREGDKKTPVGVYHVTSSLPTQRLGDFY
                     GSGAFPINYPNEWDKRQGRNGHGIWLHGTPSNTYSRPPKASDGCVVLANMDLNAISRD
                     LQIGLTPVIISNSIEWLSLDDWQNERSALQKNIDEWRKDWESKDTNRYLKHYSKKFQT
                     KDQNLEQFSQQKRQVNAGKEWIKVKLSNLSMFRNPGKEELVIVSFEQDYQSNNLNNQM
                     KKRQYWIREDGDWKIIFEGAA"
     gene            complement(1435..2511)
                     /locus_tag="PLD53_08515"
     CDS             complement(1435..2511)
                     /locus_tag="PLD53_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRC60997.1"
                     /translation="MRPNSHMPSLARLSAVFLGLLLSVSAALVNAQTLPDIQRLMKQN
                     QMPQALEKVDIYISTKPKDAQGRFLKGLILTEMGRPADAIAIFTKLTEDFPELPEPYN
                     NLAVLYAQQKQYDKARTALEMAIRTHPSYSIAHENLGDVYAKLASQAYDKALQLDSSN
                     TATQTKLSMIREMISTSSKPGAKAATPARPVAEPVKPPPAEPPKAAPTVPASKPVEKV
                     PEPAVSSKPPEAKPAETKAPVQTAPAATGDEADITKALQAWANAWSRKDVKSYLSHYA
                     RDFQTPNGMPRKKWEDERSRRIDKPGKLQVSVEDVNVAIDGEVATVKFRQQYASASFS
                     SSVIKTLVFVKSGGKWLIQQERVG"
     gene            2632..4008
                     /gene="cysS"
                     /locus_tag="PLD53_08520"
     CDS             2632..4008
                     /gene="cysS"
                     /locus_tag="PLD53_08520"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRC60998.1"
                     /translation="MLRIYNSLTREKQSFVPNEPGKVGMYVCGMTVYDLCHLGHARVL
                     VVFDMVQRWLRESGLDVTYVRNITDIDDKIIARALENKESISDLTNRFIRLMDQDATA
                     LGVEKPQYEPRATEYVPQMLSLIGLLEKNGLAYQAADGDVNFSVRKFPGYGKLSGKSL
                     DDLRAGERVEVDTAKQDPLDFVLWKHAKAGEPFWSSPWGNGRPGWHIECSAMSSDLLG
                     QHFDIHGGGQDLQFPHHENEIAQSEGAHNCDFANFWMHNGFVRVDDEKMSKSLGNFFT
                     IREILKKYDAEVVRFFILRAHYRSPLNYSDVHLDDARHALARLYTALKAVAADSVQPV
                     DWSEKHAVRFRKAMDDDFNTPEACAVLFDLAAEINRGKSPVLATQLRSLAGVLGLLQR
                     EPQLFLQASPKSAEELSVERIEALIADRVLAKREKNFAESDRIRSDLLAAGVVLEDSA
                     QGTTWRRA"
     gene            4177..4785
                     /locus_tag="PLD53_08525"
     CDS             4177..4785
                     /locus_tag="PLD53_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HRC60999.1"
                     /translation="MTQITAIGSAQASFGFENLARPGAQKASPTANEDDSSVRSGRSG
                     VSGSGKTGELSPEEQQEVQQLKQADRKVRAHEQAHLAAGAGLVRSGPSFTYQIGPDNQ
                     RYAVSGEVSIDVSPGRTPTETIPKAQQIRAAALAPVDPSPQDHSVAAKAGRMEGDARI
                     ELATQQRDEAKSAGEGTSRLYQNVEQGESEKDLLGGRLDLYA"
     gene            complement(4818..5267)
                     /gene="fur"
                     /locus_tag="PLD53_08530"
     CDS             complement(4818..5267)
                     /gene="fur"
                     /locus_tag="PLD53_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HRC61000.1"
                     /translation="MSKSTDLNSMGLKATFPRLKILELFENAEVRHLTADDVYRLLLN
                     ENIEIGLATVYRALTQFEQAGLLERHHFESGKAVFEIKAGTHHDHLVCINCGRVEEFF
                     DEEIERRQKKIAKERGFAIQEHALYLYAECTKVACQHRDTREKSRHQ"
BASE COUNT         1129 a         1418 c         1604 g         1358 t
ORIGIN      
        1 atcatgaagc cgtccatgac gcggtgaaag atcgttccgt tgtaaaagcc gtctttcgca
       61 tattgcagga agttctttac ggtgacgggt gctttctccg aatcgagttc aatgacgacc
      121 ttgcccatgc tggtctgtat ttcgacgctc ggggctgccg ccgcggctgt cgacaggatc
      181 aggcccacgg cgaacatcag gaaacggtga aaaaaggggt tcatcaggcg gctccttcaa
      241 agatgatctt ccagtcaccg tcttcacgga tccagtactg tcgttttttc atctggttgt
      301 tgagattgtt gctttgataa tcctgctcaa aagaaacgat caccagttct tctttaccgg
      361 ggttacggaa catggacaga ttcgacagct taaccttgat ccattccttg ccggcattca
      421 cttggcgctt ctgttgtgag aactgttcca ggttctggtc cttggtctgg aattttttcg
      481 agtaatgttt caggtagcgg ttcgtgtctt tgctctccca gtcttttcgc cattcgtcga
      541 tattcttctg gagtgcgcta cgctcattct gccagtcgtc cagcgagagc cattcgatcg
      601 agttgctgat gatgactggc gtcaggccaa tctgcagatc cctcgatatc gcattgagat
      661 ccatattggc cagtacaaca caaccgtcgg aagcttttgg tgggcgcgaa taggtgttgg
      721 aaggtgtccc gtgcagccag atgccatgac cgttgcggcc ctggcgtttg tcccactcgt
      781 tcggatagtt gatcggaaag gcgccactac cgtagaagtc gccgagtctt tgcgtcggca
      841 agctggaggt gacgtgatag acaccgaccg gtgttttctt gtcaccttcc cgtcttttgt
      901 cggagccgag cttgccatgc gtgatgtaat agtcggcgac aaaacgcggc cgcccgcccg
      961 cgtcggtgtc attcttgtag agatagagcc tggatttttg tgtgtctaca acaatcgcgt
     1021 atttctggtc ggggggcatc tgcagtagat aacgcggaac gtagttggac gtgtttgtgc
     1081 ggttgcggta cgccttcagg cgcgcgacga cctcatcacg caggtcggca agctgctctt
     1141 taggagcatt gctggcgttt ccgaaggttg caagaggagc gctgcgagcc aacagcagat
     1201 cgcctttgat caggtgcgcc aggcggaagt tcggataccg cttcagcagg gcttcggttt
     1261 cttcaagcgc ggtgtcgaga cggttttttt caatctcgac aagaattctt gacagctgtg
     1321 gctccgggcc agaatcagaa aaggtcgccg ccgggtgggg atttccatcg acagaagccg
     1381 ccgtacctgc cgcgcacgca gcgatcaggg tgatggcgag catcgctcgc cgcatcagcc
     1441 gacgcgttcc tgctgaataa gccattttcc tccggatttc acgaacacca gggttttgat
     1501 gaccgacgag ctgaacgatg ccgaagcgta ttgctggcga aacttgacgg tcgccacctc
     1561 accgtcaata gccacattga catcttcaac actgacctgc aatttgccag gcttgtctat
     1621 gcgccggctg cgttcatcct cccacttctt ccgcggcatg ccgttcggag tctggaaatc
     1681 tcttgcgtag tggctgagat agctcttcac gtccttgcgg gaccaggcgt tggcccaagc
     1741 ctgcaaggct ttggtaatat cagcttcgtc acccgtggct gcaggggcgg tttgaacggg
     1801 cgccttggtt tccgctggtt tggcctcggg tggctttgac gatacggctg gctccggtac
     1861 tttctcaacg ggtttgcttg ctggaactgt cggcgccgct ttgggcggct ctgctggagg
     1921 cggtttcacc ggctcggcaa ccgggcgcgc gggcgttgct gctttggcgc cgggctttga
     1981 cgacgtgctg atcatttccc tgatcatcga cagcttcgtt tgcgtggcgg tgttcgatga
     2041 gtcaagctga agcgctttgt cataagcttg gctggcgagt ttggcgtaaa cgtcaccgag
     2101 gttttcgtgc gcgatggagt agctcggatg cgtccggata gccatttcaa gcgctgtgcg
     2161 cgccttgtcg tattgctttt gctgggcgta gagaacggca agattgttgt agggttcggg
     2221 tagctcggga aaatcctcgg tcagtttggt aaatatagcg atggcatctg cagggcggcc
     2281 catttcagtc agaataaggc ctttcagaaa acgaccctga gcgtccttgg gtttggttga
     2341 gatgtagata tcgacttttt caagcgcctg tggcatctga ttctgcttca tcaggcgctg
     2401 gatatccgga agcgtttggg cgttaacgag ggcggcggaa acgcttaaga gcaggccaag
     2461 aaaaacggcc gaaaggcgcg ccagtgaagg catgtgggag tttggacgca tcgttatggt
     2521 atacttcagt cgggttggtg actcaaccga acaattctaa caagaagcac gtcgaatcga
     2581 aagtattcct tatgctcccg ttttccgtgg ggcaccctcg tgagttgaaa aatgctgaga
     2641 atctacaatt ccctgaccag ggaaaagcag agttttgttc ccaacgagcc cggaaaggtc
     2701 ggtatgtatg tatgcggcat gaccgtttac gatctgtgcc accttgggca tgctcgggtt
     2761 ctggtggtct ttgacatggt gcaacgctgg ctccgggagt ccggacttga cgtgacctat
     2821 gttcgcaata ttaccgatat cgatgacaaa atcattgctc gtgcgcttga aaacaaggaa
     2881 agcatcagcg atctgaccaa tcgtttcatt cgcctgatgg atcaagacgc aacggcattg
     2941 ggggtcgaaa agccacagta cgaaccgcgt gcaaccgagt atgtgccgca gatgctaagt
     3001 ttgatcggat tgttggagaa gaacgggctg gcctatcagg cggcagacgg tgatgtgaat
     3061 ttctcggttc gaaaattccc cggctacggc aagctttccg gcaagtcact agacgacctt
     3121 cgcgcaggtg agcgtgttga agttgacacg gcaaaacagg acccgcttga tttcgtattg
     3181 tggaagcacg ctaaggcggg cgagccattc tggtcgtcac cctggggcaa tggccggccg
     3241 ggttggcaca ttgaatgctc ggcgatgagc tccgatctcc ttggccaaca ctttgacata
     3301 catggtggcg ggcaggatct gcaatttccg catcacgaaa atgaaattgc gcagtccgaa
     3361 ggtgctcata actgcgactt tgcgaatttc tggatgcaca acggctttgt gcgcgttgac
     3421 gacgaaaaga tgtccaagtc gctcggcaat ttctttacga tccgtgagat tctcaagaaa
     3481 tacgacgccg aggtcgttcg cttcttcata ttgcgtgcgc actatcgcag cccgctgaat
     3541 tattccgatg tgcaccttga cgatgcgcgt catgctctgg cccggttgta cacggcgctc
     3601 aaggctgttg cggcggattc ggtgcaaccc gtcgattgga gtgagaagca tgccgttcgt
     3661 tttcgtaagg caatggacga cgatttcaat acacccgaag cgtgtgccgt gctgtttgat
     3721 ctggccgccg agatcaatcg cgggaaatcc ccggttctgg cgacgcaatt gcgctcactg
     3781 gccggtgttc ttggcctgtt gcagcgggag ccacaactgt tcctgcaagc ctcgcccaag
     3841 agtgccgagg aactgtccgt cgagagaatt gaggcattga ttgccgatcg ggttctggct
     3901 aaacgggaaa agaacttcgc cgagtccgac cgcatccgtt ccgatttact tgctgccggt
     3961 gtggtgctcg aggatagtgc gcagggaacc acgtggcgtc gcgcctgaga tcgatctggc
     4021 cggtgttgga actccgcaat ctcttggccc gaagcatcca atacgctggc cagaacttcg
     4081 tcactggaaa accggtcgct tttggtctag gctgaacagc gtagcccgtt gttttcaagg
     4141 tgttatcacc atggcgtctt cttgattgtt tctacgatga ctcagatcac ggcaattggt
     4201 tctgcccagg caagcttcgg ttttgagaat ctcgcgcgcc ccggtgcgca aaaagcgagt
     4261 ccgacggcga atgaagatga ttcgagtgtc cgctcgggca gatccggcgt ttccgggtcg
     4321 ggcaagacag gcgaattatc gcccgaagag cagcaggagg tgcaacagtt aaagcaggcc
     4381 gaccggaagg tgcgcgctca cgagcaggct cacctcgcgg ctggagccgg tctggtgcgc
     4441 agtggaccgt ccttcacgta tcaaataggg ccggacaatc aacgctatgc cgtatccgga
     4501 gaagtttcaa tagatgtttc acccggccgg acaccaacgg aaacgatccc caaggcgcag
     4561 cagattcgcg ctgcagcgct tgctcccgtc gacccttctc ctcaggatca cagtgtcgca
     4621 gcgaaggcag gccgtatgga aggtgatgcg cgaatcgaac ttgcgacgca gcagcgggat
     4681 gaagcgaaaa gcgcgggtga gggcacgtcc cgcctatacc agaatgtcga gcagggtgag
     4741 agtgaaaaag atttgctcgg cggtcgcctg gatctctatg cctgatccgt cggattggcc
     4801 atcgttcgcg tgatagctta ctggtgtcga gatttctcgc gcgtgtccct atgctggcat
     4861 gccaccttgg tacattccgc ataaaggtaa agtgcgtgtt cctgaatggc aaaaccgcgc
     4921 tcctttgcga tttttttctg gcgacgctcg atctcctcgt cgaaaaactc ctcaacgcga
     4981 ccgcagttga tacacacgag atggtcgtga tgtgtcccgg cttttatttc gaataccgcc
     5041 tttcctgatt cgaaatggtg gcgctcaagc aagccggctt gttcaaattg ggtgagcgcc
     5101 cggtaaaccg tcgccagccc gatctcgata ttctcgttga gcagcaggcg atagacatcg
     5161 tcggccgtca gatgacgcac ttcagcattt tcgaaaagct cgagtatttt caggcggggg
     5221 aaggttgctt tgagccccat gctgttcagg tcggtggatt tgctcatggc taggccttgt
     5281 cgtcggataa aggaggcgtg cttatcgggt atgatatacc gcctgacacg acatgaagag
     5341 gctctggcgt cattcagcag accatattcg acactttact tagaaacatc caacgcatga
     5401 ctttttcacg acttgccggt acggtgttct tgatagccgc tatttccggt tgttcctcgg
     5461 tgccacgtat cgtcaacgag tacaagattg acgttcagca gggcaacgt
//
LOCUS       DAORPM010000166         5484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4147_length_5484_cov_11.273531, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000166 DAORPM010000000
VERSION     DAORPM010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4147_length_5484_cov_11.273531"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLD53_08535"
     CDS             complement(<1..198)
                     /locus_tag="PLD53_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61001.1"
                     /translation="MTHRTNAFLPFLIGAVFTVCSLSSLAMPKAAEHEDAPVTMGADV
                     PKPKRSTKGAEKPVAKKGKPAT"
     gene            complement(267..1856)
                     /locus_tag="PLD53_08540"
     CDS             complement(267..1856)
                     /locus_tag="PLD53_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRC61002.1"
                     /translation="MKFRLWLACCGFSIGLFGAASPALSADVGALYKTHCAACHGENR
                     FGGIGPALLPENLARLRKPEALKVILEGRVATQMPGFGDRLNAEEAQQLADFIYTEVL
                     PAPEWGELQIRQSHIVNADAKHLSSKPGPGLKGADPLNLFIVVETGDHHVSVLDGDTF
                     ERLYRFPSRYALHGGPKFSPDGRFVYFASRDGWITKFDIYNLTIVAEIRAGLNTRNAA
                     VSGDGKWVMVANYLPHSLVLLDAEDLSLVRIIPTLNAAGDKTSRVSAVYDAAPRKSFV
                     AALKDVPEAWEISYDPNAEPVAEGLVHDYRYKEGVFKPGQFHPKRTMLKDYLDDFFFT
                     QDYAFILGSSRSASKGQVIYLDGRKKIADLDLPGMPHLGSGITWQWKDPSGKERTVMA
                     SPNLNEGLITVIDLNTYENIKQIKTLGPGFFLRSHENSRYAFTDSMMSKEKNNVLQVI
                     DKQKLEVVAQIKADPGQTLAHVEFTRDGRYAVASLWEMDGALLVINTETLKVVKRIPA
                     KKPVGKYNVWNKITRSEGTSH"
     gene            complement(1856..3049)
                     /gene="nirJ"
                     /locus_tag="PLD53_08545"
     CDS             complement(1856..3049)
                     /gene="nirJ"
                     /locus_tag="PLD53_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HRC61003.1"
                     /translation="MFRISQYMQEIASPTPLGQKRNPPAPVVIWNLIRRCNLTCKHCY
                     SISADKDFPGELTTGEIFKVMDDLKGFRVPVLILSGGEPLLRPDIFDIAQHAKAMGFY
                     VGLSSNGTLIDAGNIDRIAECDFNYVGVSLDGIEETHDKFRRMEGAFEASLRGIRLCR
                     DLGLKIGVRFTMTQDNAHDLPKLLKLVEDEGIDRFYFSHLNYAGRGNKNRKDDAQHKL
                     TRWAMDLLFDTCWEYEQRGLNKEFTTGNNDADGVYFLYWVRKNFPEKAEHIHAKLAQW
                     GGNSSGVNVANIDNLGNVHPDTMWWHYTLGNVRQRAFSEIWADTSDPLMAGLKRFPRS
                     VTGRCGECLHFDICNGNTRVRAQQLTGDPWAEDPGCYLDDEEIGVAPGSERLSVTPYI
                     RIRRA"
     gene            complement(3197..4939)
                     /locus_tag="PLD53_08550"
     CDS             complement(3197..4939)
                     /locus_tag="PLD53_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRC61004.1"
                     /translation="MPDQNTPARIVLALLKPYRNRILLAAIALVVAAMAMLGVGQGLR
                     AVIDRGFSAGDPAWLDRALMAMFGIIVLLAAATYTRFYNVSWLGERVTADLRRRVFDH
                     LLTLPPSYFEQGRTGAVISRLTSDTTLIENAIGSSLSIALRNILILIGGLTMLFTTNL
                     KLTLMVLAGVPLVVTPIVLFGRRVRRLARISQDRVADLGNRIDETIHEIRIVQAYGHE
                     NADRRDFADLVERGFTTAVARIRNRATLVAAVMVLVFGAIALILWVGGHDVLLGRITP
                     GQLSAFVFYAALVAGSVGALSESWGDLQRAAGATERLLELLAAQPDVGMPLHPLPLPA
                     RARGEITFEGVDFRYPSRPDTPALDSFSLSVSPGESVALVGPSGAGKTTVFQLLLRFY
                     DPQSGSLCLDGIPLASADPQAVRRCIALVPQEAVIFATSVLENVRYARPEASREEVRE
                     VCAAAYADDFVSRLPEGYETELGERGVRLSGGQRQRIAIARALLADRPILLLDEATSA
                     LDAESERLVQAALEKLMKNRTTLVIAHRLATVQQANRIVVMDQGRIVETGSHAELVRQ
                     DGLYAHLARLQFTS"
     gene            5043..5438
                     /locus_tag="PLD53_08555"
     CDS             5043..5438
                     /locus_tag="PLD53_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRC61005.1"
                     /translation="MSSLPMAAFVTLLTVLLLFVTALNVGRARVRYGIKAPAVSGHEV
                     FERAFRIQMNTLENTLLMLPALWLYAGLIGDRGAGMMGIVWLVGRVWYAVAYQINPDK
                     RGGGFGLAMLAFAGLWFGALWGVVRVMAH"
BASE COUNT         1089 a         1569 c         1695 g         1131 t
ORIGIN      
        1 ggtagcaggt ttgcctttct tggcaaccgg tttttcggcc cctttggtgg aacgctttgg
       61 cttgggcaca tcggcaccca tggtgactgg cgcgtcttca tgctcggctg cttttggcat
      121 ggcgagcgac gacaggctgc aaacagtgaa cacggctcca atcagaaagg gcaggaaggc
      181 gtttgttcgg tgtgtcatgt gtgggctccg gtattgcttt cagtgtcggc gggacgattc
      241 aaacaggtaa gagggttggc agcacatcaa tggctggtgc cttcggagcg ggtgatttta
      301 ttccagacat tgtatttgcc aaccggtttt ttcgccggaa tacgtttgac cactttcagt
      361 gtttcggtat tgatgacgag cagggcgccg tccatttccc acaggctggc cacagcataa
      421 cgcccgtcgc gcgtgaattc gacatgcgcc agtgtctgac ccggatcagc cttgatctgg
      481 gcgaccactt cgagtttctg cttgtcgatc acttgcaata cgttattttt ttccttgctc
      541 atcatcgaat cggtaaaggc ataccggctg ttttcgtgac tgcgcaggaa gaagccaggg
      601 ccgagagtct tgatctgttt gatgttctca taagtattga gatcaataac cgtgatcagt
      661 ccttcgttga gattgggcga ggccatcacc gtgcgttcct tcccgctcgg gtccttccac
      721 tgccaggtga tgcctgaacc gagatgaggc attcccggca agtcgagatc ggcgattttc
      781 ttgcggccat cgagatagat gacctggcct ttgctggctg agcgcgaact gccgagaatg
      841 aaagcgtagt cctgtgtgaa gaagaaatcg tcaagataat ccttgagcat tgtccgcttt
      901 ggatggaact gccccggctt gaagacacct tccttgtatc tgtagtcgtg caccaagccc
      961 tcggcaaccg gctcggcatt tgggtcgtag ctgatctccc aagcttcagg aacgtccttc
     1021 agcgcggcga cgaagctctt gcgcggcgca gcgtcataca cggccgatac gcgcgaagtc
     1081 ttgtcgcccg cagcattcag ggtcgggatg atcctgacca ggctgaggtc ttcagcatcg
     1141 agcagcacca ggctgtgtgg caagtaattg gcgaccatga cccacttgcc gtcaccggaa
     1201 acggccgcgt tgcgcgtgtt caacccggcc cggatttcag cgacgatggt caggttgtag
     1261 atgtcgaact tggtaatcca gccatcgcgc gaagcaaaat agacaaagcg cccatccggc
     1321 gagaacttcg ggccgccgtg caaggcatat cggctgggga atcgataaag gcgctcgaag
     1381 gtgtcgccat ccagcaccga cacgtgatgg tcgccggttt caaccacgat gaaaaggttg
     1441 agcggatcgg ctcctttgag tccggggccg ggtttggacg aaaggtgttt ggcatcggcg
     1501 ttgacaatgt gtgattggcg tatctgcagc tctccccatt ccggcgccgg cagcacttcc
     1561 gtgtagatga aatcggccaa ctgttgcgct tcctcggcgt tcaggcggtc gccaaagcct
     1621 ggcatctgcg tcgccacgcg cccttcgaga atcaccttga gggcttccgg tttgcgcaag
     1681 cgcgccagat tctcgggcag cagggcaggg ccgatgccgc caaaccggtt ctcgccatga
     1741 caggcggcgc aatgcgtctt gtagagcgct ccaacgtcgg ccgatagagc cggtgatgcg
     1801 gcgccaaaaa gcccgatcga gaacccgcag cacgccagcc acagacgaaa tttcatcaag
     1861 ctctcctgat tctgatgtag ggagtgacgc tcaatcgctc gcttcccgga gcaaccccga
     1921 tttcctcgtc gtcgaggtag cagcccgggt cttcggccca ggggtcaccc gtcaattgtt
     1981 gcgcgcgaac gcgggtgttg ccgttgcaga tgtcgaaatg caggcattcg ccgcagcggc
     2041 cggtgacgct gcgcggaaac cgcttgaggc cggccatgag cgggtcggac gtgtccgccc
     2101 aaatctcgga aaacgcccgt tggcgaacat tgcccagcgt gtaatgccac cacatggtat
     2161 ccggatgcac attgcccaga ttgtcgatat tggcgacgtt cacgcctgat gagttgccgc
     2221 cccactgtgc cagcttggcg tgtatatgct cggccttctc ggggaaattc ttcctcaccc
     2281 agtaaaggaa atacacgccg tcggcatcgt tgttgccggt tgtgaactcc ttgttgaggc
     2341 cgcgctgttc atattcccag caggtatcga agagcagatc catggcccag cgcgtcagct
     2401 tgtgctgggc atcgtccttg cggtttttgt tgccgcgccc ggcatagttg aggtgtgaaa
     2461 aatagaagcg gtcgatgcct tcgtcctcga ccagtttcag cagcttgggc agatcgtgcg
     2521 cgttatcctg ggtcatggtg aaacgcacgc cgatcttcag ccccagatca cggcacaggc
     2581 ggatgccgcg gagcgaggcc tcgaaagcac cctccattcg gcggaacttg tcgtgcgttt
     2641 cctcaatgcc atcgagtgaa acgccgacat aattgaagtc gcactcggcg atgcgatcga
     2701 tgtttccggc atcgatcagc gtgccgttgg acgagagacc aacgtaaaag cccatcgcct
     2761 tggcgtgttg cgcaatatcg aaaatatccg gacgaagcag tggttcgcca ccggacagaa
     2821 tgagcaccgg aacgcgaaag cccttgaggt cgtccatcac cttgaaaatt tcgccggtag
     2881 tcagttcacc ggggaaatcc ttgtcggcgg aaatcgaata gcagtgcttg caggtcaggt
     2941 tgcagcgtcg aatcaggttc cagatgacga ccggcgcagg cggattcctt ttttgcccga
     3001 gcggagtggg cgaagcgatt tcctgcatgt attgcgagat acggaacatg gttgggctca
     3061 ctcgggagga cgcgtcattc tatagcagac gcttcggccc ggtattgaca tcgatcaatc
     3121 gaccgaggat ttgccaccat gggtatatcg ctgagcgcca gacgcagtat tccgacagcg
     3181 gtttgcgccg tttttctcag gacgtgaact gcaagcgtgc cagatgagcg taaagcccat
     3241 cctgccgcac cagttcagcg tggctgcctg tttcgacaat gcgcccctgg tccatgacca
     3301 cgatgcggtt ggcctgctgc accgtggcga gccgatgggc aataaccagc gtcgtacggt
     3361 tcttcatcag cttctccagc gcggcctgga ccagacgttc gctttcggcg tcaagcgccg
     3421 aggttgcctc gtcgagcagc agaatagggc ggtcggcaag cagcgcgcgg gcaatggcga
     3481 tgcgctggcg ctggccaccg gaaagtcgca cgccgcgttc acccagttcg gtttcatacc
     3541 cttcgggcaa gcggctgaca aaatcgtcgg cgtaggcggc cgcgcaaacc tcgcgtactt
     3601 cttcccggct ggcctccggt cgggcatagc gcacgttctc cagtacgctg gtggcgaaga
     3661 ttacagcctc ctggggtacc agggcgatgc agcggcgcac tgcctggggg tccgcagacg
     3721 caaggggtat tccgtcgaga caaaggctgc ccgattgggg atcgtagaag cgaagcaaaa
     3781 gctggaaaac cgtagttttg cccgcgccgg acggaccaac cagggcgacg ctttcaccgg
     3841 gtgaaacgga aaggctgaag ctgtccaggg cgggtgtatc aggccgggaa gggtagcgga
     3901 agtcgactcc ctcgaaagtg atttctccgc gcgctcgcgc gggcagggga agcggatgca
     3961 gcggcatgcc cacatcgggt tgggccgcga gcagttccag caggcgctcg gtggcgccgg
     4021 cggcccgctg caaatcgccc caggattcgg acagggcgcc aaccgagccg gcaaccagcg
     4081 ccgcatagaa gacaaaggcc gagagctggc cgggggtgat gcggccgagc agcacatcgt
     4141 gcccgccgac ccacaggatc aaggcgatcg ctccgaatac cagcaccatg acggccgcta
     4201 ccagcgtggc acggttgcgg atacgcgcga ccgccgtcgt gaaacctcgc tcaaccagat
     4261 cggcgaagtc gcgccggtcg gcgttctcat gcccataggc ctggacgata cggatttcgt
     4321 ggatcgtctc gtcgatccga ttgcccagat ccgccacccg gtcctggctg atgcgcgcca
     4381 ggcgtcgcac tcgacgtccg aacagcacga tgggtgttac caccagcggt acgccggcca
     4441 gcaccatcaa ggtcagcttg agattggtgg tgaacaacat ggtcagcccg ccgatcagga
     4501 tcaggatatt gcgcagggcg atcgacaggc tggaaccgat agcattctct atcagagtcg
     4561 tgtccgaggt aagccgagaa atcacggcac cggtgcgacc ctgttcgaaa tatgagggag
     4621 gcagtgtcag caggtggtcg aagacgcgcc gacgaagatc cgcagtgacg cgctcgccga
     4681 gccacgagac attgtagaag cgggtgtaag tcgccgccgc gagcaggacg atgatgccga
     4741 acatggccat cagggcgcga tcaagccagg ccgggtcacc ggcggaaaac ccccggtcaa
     4801 tcaccgctcg caatccctga ccgacgccca gcatggccat ggcagcaacc accagggcga
     4861 tggccgcaag cagtatgcga ttgcggtacg gcttgagcaa agccaggaca atgcgggcag
     4921 gagtgttttg gtcgggcatg gttcggtttc gtgaatcggc ctgcattgta agtcccgatt
     4981 ggcatggggt atagttccga gttcgcccag tgccacgcta gctcaattct caaggagcaa
     5041 ccatgtcttc ccttcccatg gcagctttcg ttaccctgct gacggttctg ctgttgttcg
     5101 tcacggcgct gaatgtcggt cgggccagag tacgctacgg catcaaggct cccgccgttt
     5161 ccgggcatga ggttttcgag cgcgcgttcc gcatccagat gaatacgctg gagaacacat
     5221 tgctgatgtt gccggccttg tggctgtatg ccggactcat cggcgaccgc ggtgccggca
     5281 tgatgggaat tgtctggctt gtcggccgcg tctggtatgc agttgcctat cagatcaatc
     5341 cggacaagcg cggcggcgga ttcgggctcg ccatgctggc ctttgccgga ctctggtttg
     5401 gggcgctatg gggcgtcgtg cgggtaatgg cgcactgaag cgcatcgagt cattcaacgt
     5461 aggcatctgg ccatggacat ccac
//
LOCUS       DAORPM010000167         5482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4149_length_5482_cov_11.690989, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000167 DAORPM010000000
VERSION     DAORPM010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_5482_cov_11.690989"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /gene="rpmE"
                     /locus_tag="PLD53_08560"
     CDS             <1..180
                     /gene="rpmE"
                     /locus_tag="PLD53_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRC61006.1"
                     /translation="EVTCSCGSVFKTQSTMKKALHVEVCSSCHPFYTGKQKIIDTAGR
                     VEKFNRKYGAMHKPA"
     gene            277..>1665
                     /locus_tag="PLD53_08565"
     CDS             277..>1665
                     /locus_tag="PLD53_08565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRC61007.1"
                     /translation="MPFLPEHPRFKGITLPPSGWVLAILLATYIFTGLIGHDPWKHDD
                     AVSIGVAYDMVVNGNWLIPQLAGNPYPDAPLYYWTSAITAHLFSWLMPLHDAARLSSG
                     IFTLLALEFILLAARELHGRENAAAAPLILAGSIGFLFHAHEAQPMLAALTAHTAAYW
                     ALTLLPRRPALATLAFGAAIALGFLANGLLPVLTLLPVTVYTLWQSGTRRTCALLVTI
                     AIALALFLSALWLLPLRMASPYFFSIFLQSELDSLTKSVSFFSNTLRYLNLLLWYAWP
                     ALPLAAWALWSKRRILRSRPMALPIFSFLSVLLILGLSVEVRSSAALLLLPPLVLLAV
                     PGVATLRRGAANAFDWFGMMTFTFLMTVVWIVWSAMVFGWPRQLARQAVRLEPGFVGH
                     FSLMAVSVSMLATMIWFWLIITSPRSPMRGIMHWMAGLTVFWLLIVALWMPWIDYGKS
                     YRPLSASLAKKLP"
     assembly_gap    1666..1765
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1766..2298)
                     /gene="orn"
                     /locus_tag="PLD53_08570"
     CDS             complement(<1766..2298)
                     /gene="orn"
                     /locus_tag="PLD53_08570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HRC61008.1"
                     /translation="MAQDANHLVWLDMEMTGLDPENERIIELAMIVTDSELLTLAESP
                     VWVVHQSDAQLAAMDEWNKKTHGRSGLIEKVKASALDEAMVEAQALAFLQEHVPKGAS
                     PMCGNSIGQDRRFMVRYMPLMEAWFHYRNIDVSTLKELCRRWKPELAKGFVKKSNHTA
                     LADIRESIEEMKYYREH"
     gene            2343..3581
                     /locus_tag="PLD53_08575"
     CDS             2343..3581
                     /locus_tag="PLD53_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRC61009.1"
                     /translation="MINNFSLAFLTALSLTIVLRLYLAYRQISYVLARRCKVPKQFSE
                     RVELSAHQKAADYTAQRTRFGILTLAVETGLLLAFTFGGGLQALHDFWSVRLSGLAYG
                     IAMIFSVMFVSGLVDLPLSVYSQFVIEEKFGFNRMTLKLFIADLLKQTALGIAIGTPV
                     LVAVLWLMAQRGSFWWLYVWLFWCVFNLLILFIYPTWIAPLFNKFTPLDDAPLKARIE
                     ALLQRCGFATSGLFVMDGSRRSNHGNAYFTGFGKTKRIVFFDTLLGRLQPVEMEAVLA
                     HELGHFRHRHVIKRIVLIFALSLAFLAALGQLIDADWFFSGLGVSAKDTVLALILFFM
                     VTPVFTFLLTPLTSLISRRHEFEADRYAAEHASADGLIEALVKLYEDNAATLTPDPLH
                     SLFYDSHPPASLRIARLNGC"
     gene            3604..4467
                     /gene="rsgA"
                     /locus_tag="PLD53_08580"
     CDS             3604..4467
                     /gene="rsgA"
                     /locus_tag="PLD53_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRC61010.1"
                     /translation="MIEGTIVSAHGRQYLVELTAGETLLCFPRGKRSEVACGDLVHVQ
                     RSSDTQGVIDSILPRSTLLYRSNEFRQKLIAANVTQIIIVVATEPPYSDELISRCIVA
                     AESQDMKTLIVFNKCDLSDRIALTSMALAPFEKIGYPVLRLSARSDAEALRPYLSGQT
                     SVLVGQSGMGKSTLTNALLHDANAPTREISTALKAGKHTTTHATLYRFDTHSALIDSP
                     GLQEFGLRHLSKNAIEHGFLELRSLQGQCRFRNCHHGREPDCAVHSAVRRGEIDPRRY
                     AAFRTLCAEVG"
     gene            4486..4764
                     /locus_tag="PLD53_08585"
     CDS             4486..4764
                     /locus_tag="PLD53_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HRC61011.1"
                     /translation="MSVITFQRKHGKSESDARAAAENMALQLKKEFDLEYVWDGEVMQ
                     FKRPGVSGELTLDKQNVILTIRLGLFLSALRPRIEREVHRFLDENFQA"
     gene            complement(4797..5333)
                     /locus_tag="PLD53_08590"
     CDS             complement(4797..5333)
                     /locus_tag="PLD53_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61012.1"
                     /translation="MTPHSEEPEIAAEPPVAKSNISLVVVLSTSLGILLTVLAAGAYL
                     HFQKSSAMESELAAANDVLKEKNLLLDEMKAQIEVLSKQVHVLKEHSVARSHTVREND
                     KNAESADPAAGSVEDASKSPGEKGKTVSPETPDAQSAKKKKPEAENCELVGKSPEEQA
                     ATLRRCVGMMDAPKGALR"
BASE COUNT         1077 a         1629 c         1353 g         1323 t
ORIGIN      
        1 gaagtcacct gctcctgcgg cagcgtgttc aagacccagt cgacgatgaa gaaggcgctt
       61 cacgttgaag tatgctcttc ctgccatccg ttctataccg gcaagcagaa gatcattgat
      121 actgcaggcc gcgtcgaaaa attcaataga aagtatggcg caatgcacaa gccagcctga
      181 atctgaccaa gagttcatgc ggactcagcg aaaaggcagc cgcacggctg cctttttcat
      241 ttagcccaac agatatccaa taaccataac ctggccatgc catttctacc ggaacatcca
      301 cgcttcaagg ggattaccct tccgcccagc ggctgggtgt tggccatcct gctggcaacc
      361 tacattttta ccggcctgat cggccatgat ccgtggaagc acgacgatgc agtctcgatc
      421 ggtgtcgcct acgatatggt cgtcaacgga aactggctga ttccgcagct cgcaggaaac
      481 ccttacccgg acgctccgct ctattactgg acgtcggcga tcaccgccca tctcttttcc
      541 tggctgatgc cgctacacga cgcggcgcgc ctaagcagcg gaatattcac cctgttggcg
      601 cttgagttca tccttctcgc tgctcgcgaa ctgcatggtc gcgaaaacgc ggccgccgcc
      661 ccattgattc ttgccggaag cataggattc ctgttccacg cacacgaagc gcaacctatg
      721 ctcgccgcgc tcaccgctca cacggcagct tactgggcgc tgaccctgct cccacgacgc
      781 cccgctcttg caaccctggc ctttggtgca gccatcgccc ttggttttct ggccaacggt
      841 ctgctccctg tgctgactct gctgcccgtc accgtgtata ccttgtggca atcgggaacc
      901 aggaggactt gcgcattgct cgtgaccatt gccatcgccc tggcgctttt cctctccgct
      961 ttgtggttgc tgcccctgcg aatggcttcg ccctatttct tctcgatctt cttgcagagc
     1021 gaattggatt ccttgaccaa gtccgtatcg ttcttttcga acacgcttcg ctacctgaac
     1081 ttgctgctct ggtacgcctg gcctgcgcta ccgctggcgg catgggcatt gtggtcaaag
     1141 cgtcgcattc tccgcagtag gccaatggca ttaccaatat tctctttctt atcggtactg
     1201 ctcattctag gtctgagcgt agaggttcgc agctctgcgg cactattgct actgccgccg
     1261 ctcgttttgc tggccgtccc tggcgttgcc acgctacggc gcggtgcggc gaatgcattc
     1321 gactggttcg gaatgatgac ctttactttc ttaatgaccg tcgtctggat cgtctggagc
     1381 gcaatggtct tcggctggcc tcggcaactg gctcgccagg ccgttcgact ggaacccggt
     1441 ttcgtcggcc acttcagcct catggcggta tcggtctcga tgcttgcaac gatgatatgg
     1501 ttctggctga tcatcaccag tccacgctcg ccgatgcgcg gcatcatgca ctggatggca
     1561 ggcctgacag tgttctggct acttattgtg gcactctgga tgccctggat cgactatgga
     1621 aaatcttacc gtccgctctc tgcctctctg gccaaaaaac tgccgnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnaaatg ctcgcggtag tatttcatct cttcaattga
     1801 ttcacgtata tcggcaagcg ccgtgtgatt ggatttcttt acgaatccct tggcaagctc
     1861 tggcttccag cgccggcaca gctccttgag cgtgctgaca tcaatgttac ggtaatgaaa
     1921 ccacgcttcc atcagcggca tgtagcgcac catgaagcgg cgatcctgtc cgatcgaatt
     1981 gccgcacatc gggctggcgc ccttgggtac atgctcctgc aggaacgcaa gcgcctgcgc
     2041 ctcgaccatc gcctcgtcaa gcgcagaggc cttgaccttt tcgatcagac cggagcggcc
     2101 atgcgtcttc ttgttccact cgtccatggc tgcaagctga gcatcggact gatgaacgac
     2161 ccataccggc gattcagcaa gggtaagcag ttcgctgtcg gtaacgatca tcgccagttc
     2221 gatgatgcgc tcgttctccg gatcaaggcc ggtcatttcc atatccagcc aaacaaggtg
     2281 atttgcgtcc tgtgccatat aatctgtccg aactcttcaa accggcattc tgtcacagct
     2341 tgatgatcaa taatttctcg ttggcctttc tcaccgcact ttccctgacg atcgtgcttc
     2401 gcctgtacct ggcttaccgt cagatttcct acgttctcgc tcgccggtgc aaggtgccca
     2461 aacagttttc tgagcgtgtc gagctttcag cgcatcagaa ggcggcagac tacacggccc
     2521 aacgaacgcg cttcggaatt ctgacgcttg ccgttgaaac cggtcttctt ctggcgttca
     2581 cgtttggggg tgggttgcag gcacttcatg atttctggtc tgtcaggctt tccgggctgg
     2641 cctatggcat agcaatgatt ttcagcgtca tgttcgtatc gggccttgtc gatcttccct
     2701 tgtccgtgta ctcgcaattc gtcatcgaag agaaatttgg cttcaaccgc atgactctca
     2761 agttattcat cgccgatctt ctcaaacaaa ccgcacttgg cattgccatc ggcacgccgg
     2821 tactggtggc cgttctatgg ctgatggcgc aaagaggcag cttctggtgg ctctatgtct
     2881 ggctattctg gtgcgttttc aatctactta ttctcttcat ctatccgacc tggatcgcac
     2941 cattgttcaa caagttcacg ccactggatg atgcaccgct caaagcgcgt atcgaagccc
     3001 tcctccagcg ttgcggtttt gccacttccg gcctgtttgt catggatggt tcccggcgct
     3061 caaaccatgg caacgcctac ttcaccggct ttggaaaaac caagcgcatc gttttcttcg
     3121 acaccctgct tggccgactg caaccggtcg aaatggaagc tgttctggcg cacgaacttg
     3181 ggcatttcag acatcgtcac gtaatcaaac gcattgtcct gatctttgcc ttgtcgctgg
     3241 cctttctcgc cgcactcggc cagctgattg acgcggattg gttctttagc ggactcgggg
     3301 tatcagcgaa ggacaccgtt ctggcgctga ttctcttctt tatggtaacg ccggtcttca
     3361 ctttcctgct gacaccgctg accagcctga tctcgcgtcg ccatgagttc gaagcggatc
     3421 gctacgccgc cgaacatgct tcggctgatg gtctgatcga agctctcgtc aagctctatg
     3481 aagacaatgc ggcgacactt acgcccgacc cgctgcactc tctgttctac gattcgcacc
     3541 cgcccgcatc gctgcgcatc gcgcgcctga acggatgctg agttcggcac gattagcatt
     3601 ttgatgatcg aaggtaccat cgtctccgcc catgggcgtc aatatctggt tgagcttaca
     3661 gcaggtgaaa cgctgctctg cttcccccgc ggcaagagaa gtgaagtggc ttgcggtgat
     3721 ctggtccacg ttcaacgcag cagtgatacc cagggtgtaa ttgactcgat tctgccacgc
     3781 agtacgctgc tttatcgctc gaacgagttc cggcagaaac tgatcgccgc aaacgtgacg
     3841 caaatcatca tcgtcgtcgc taccgagccc ccatattccg acgaactgat atcgcgatgc
     3901 atagttgccg cggaaagcca ggacatgaag acactgatcg ttttcaacaa atgtgacctg
     3961 agtgaccgta tagcactaac cagcatggca ttggcgccat tcgaaaagat aggctacccg
     4021 gtactcaggc tttcggctcg ctccgacgcc gaggcgctac gcccatacct atcaggtcag
     4081 acaagtgtac tcgtcggtca gtccggcatg ggcaagtcaa cgctgaccaa cgcgctgctg
     4141 catgatgcca atgccccgac acgcgagatc tccacagcgc taaaagccgg caaacacacc
     4201 acgacacacg ccacgctcta tcgcttcgat acccactccg cgcttatcga ctctcccgga
     4261 ctgcaagagt ttggcttgcg ccacctctcg aaaaacgcca tcgagcatgg gtttctggaa
     4321 ctccgctcac ttcaaggtca atgtcgtttc cgcaactgcc accatggccg cgagccggat
     4381 tgtgcagtac attctgctgt caggcgcggc gaaattgacc cgcgccgcta tgcggctttc
     4441 cgcactctgt gtgctgaagt cggctgaccc tacaggaatc tcatcatgag tgtcattact
     4501 ttccagcgca aacacggaaa gagcgaatcc gatgcccgcg ccgcagccga gaacatggcc
     4561 ttgcagctca agaaagagtt cgacctcgag tacgtgtggg atggtgaagt catgcaattc
     4621 aagcgtcccg gcgtatcggg tgaactcaca ctcgacaagc aaaatgtcat cctgacaatt
     4681 cgtctcggcc tttttctctc agcgctcaga ccaagaatcg aacgtgaagt gcaccgattc
     4741 cttgacgaga acttccaagc ctgagccaag ccgacagaat tgaattgact tcagcgttaa
     4801 cgcagcgccc ccttgggcgc gtccatcatg cctacgcaac gccgcagcgt cgcggcttgc
     4861 tcttcagggg atttaccaac cagttcgcag ttctccgctt ccggtttctt tttcttggct
     4921 gactgtgcgt caggtgtttc gggagacacg gtctttccct tctcaccggg cgacttcgat
     4981 gcatcttcca cggaacctgc tgcggggtca gcgctctccg cgttcttgtc attttcacgc
     5041 actgtatgcg aacgagcgac cgaatgctct ttcaggacat ggacctgttt ggaaagcact
     5101 tcgatctgcg ccttcatttc gtcaagcagc aggttctttt ccttgagtac gtcatttgcc
     5161 gcagcaagtt cggattccat agcactgctc ttctggaaat gcagatacgc acccgccgcc
     5221 aataccgtca gcaaaatgcc gagtgacgtg gacagaacga cgacaaggga aatattcgat
     5281 ttggcgactg gtggctctgc ggcaatttcc ggctcttcac tgtgcggtgt cacggtgttg
     5341 tcctttgact atccgtttgg caagcgatga tgatcaaata caggatcaca ctcgcgcata
     5401 acgtatccca aaggaaacac tttggcaatc cgcgcagatc tgtccaagct cccagggagt
     5461 cccatcgcgc acccgtgcaa cg
//
LOCUS       DAORPM010000168         5463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4173_length_5463_cov_10.905880, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000168 DAORPM010000000
VERSION     DAORPM010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4173_length_5463_cov_10.905880"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /gene="ubiB"
                     /locus_tag="PLD53_08595"
     CDS             complement(<1..1193)
                     /gene="ubiB"
                     /locus_tag="PLD53_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HRC61013.1"
                     /translation="MRIFRLAKIVGIVSRYGIDEMIFEHEPSGTLAALSRIVNFWRRN
                     TLPRAVRLRLALESLGPIFVKFGQVMSTRRDLLPLDIADELAKLQDRVPPFSPGLALA
                     QIEEAYGRPASEVFAEFNATPVASASVAQVHFARLHPEDGGHEVAVKILRPNMRGIIA
                     HDLALLDTLAGLLEKVWSDGKRLKPREVVAEFAKYLYDELDLMREAANCSQLRRNFLG
                     SKLLLVPSVYWDHCTTTVMVMERMHGIPISQTTALTAAGIDLPTLSRAGVEIFFTQVF
                     RDGFFHADMHPGNIFVASDEVHHGKYVALDFGIVGTLTDVDKNYLAQNFLAFFQRDYK
                     RVAMAHIEAGWAPADTRVDEFESAIRAVCEPIFDRPLHEISFGRVLLRLFQTSRRFNV
                     DIQPQL"
     gene            complement(1258..1524)
                     /gene="minE"
                     /locus_tag="PLD53_08600"
     CDS             complement(1258..1524)
                     /gene="minE"
                     /locus_tag="PLD53_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRC61014.1"
                     /translation="MSLFSLIFGSKPKTATIAKERLQLIIAHERSGQNTKPDFLPALQ
                     QELIAVISKYVLVNPDDIKVSLEKQGNYEVLEVNIVLPEPVSAR"
     gene            complement(1527..2348)
                     /gene="minD"
                     /locus_tag="PLD53_08605"
     CDS             complement(1527..2348)
                     /gene="minD"
                     /locus_tag="PLD53_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRC61015.1"
                     /translation="MTRIVVVTSGKGGVGKTTTSASFASGLALRGFKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLVNVLKEEATLTQALIKDKHCSNDNLFVLPASQTRDKDALTEE
                     GVEKVLKELEHMGFDYVVCDSPAGIERGAVMALTFADEAIVVTNPEVSSVRDSDRILG
                     IIQAKSRRAISGDEPVKEHLLITRYSPKRVEDGEMLSYKDVQEILRVPIIGVIPESEV
                     ILQSSNQGSPAVHVSGSDVSEAYLDVVSRFLGETPPLRFVDYTKPGLFKRMFGGK"
     gene            complement(2439..3245)
                     /gene="minC"
                     /locus_tag="PLD53_08610"
     CDS             complement(2439..3245)
                     /gene="minC"
                     /locus_tag="PLD53_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRC61016.1"
                     /translation="MARTSKTSSLIEFKGTTLPVVSVTLHSLQSGALAGAATELFGEE
                     AFFDRDAAILELDRIDAGEVSEAVDWAALCSTFSQHGLRVIGVRGGSDALHESAANAG
                     LPSFTAFKRSSRRIEKSAPAAAVQDEPVQEAAVVAEAVSEPPVVLPASSPTLVIDRPL
                     RSGQQIYARGGDLVVLAAVNAGAEVIADGSIHIYAPLRGRALAGASGARDARIFTTRF
                     EAELVSIAGIYRTFEAGIPDQLASQSAQIKLIETQGGTDSRLSIEALKTN"
     gene            3547..4689
                     /locus_tag="PLD53_08615"
     CDS             3547..4689
                     /locus_tag="PLD53_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC61017.1"
                     /translation="MKPIHTENNAGLRLVQARVCLAVLLGVLLAACGKAPGEADASTQ
                     PVKAALTVSTTVPQRLDWPETLRANGNIAAWQEAVIGPELSNYRITEVNANVGDVVKK
                     GQVLARIAADTVENELSESRAAVAEAEATLAEARANNARAKQLRDKGFYSPQQGIQTQ
                     TVADTALARLNAARARLQTAQLRRSKAAVLSPDDGIISARSATVGTLTQPGQELFRLI
                     RGSRLEWRAEVTEAEIARLKPGQAAALSSPTGDVLEGVVRTVAPTVDPQTRTALVYVD
                     LPANAAQALSAGMFIRGEFSLGQNPALTLPQSAVVLREGFAYVFLLENDKVSQLKIET
                     GRRIGDRIEVSGLDPQAEVVASGAGFLADGDTVHVVAAQPVPVTPR"
     gene            4686..>5463
                     /locus_tag="PLD53_08620"
     CDS             4686..>5463
                     /locus_tag="PLD53_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC61018.1"
                     /translation="MNVSTWSIRNPIPAVLLFLMLTLLGLMAFRGMKIQQFPDIDLPT
                     VTVTASLPGAAPAQMETEVGRKIENAVATLQGVKHIYTRAQEGTVVVSVEFRLEKPTQ
                     EGIDDVRDAVSRIRADLPGELKDPVIARVNLAGAPILTYTVASSRMDDEALSWLVDNT
                     LTKAMLSVRGVGAVARVGGVSREIRIELDPARLLALHATAADVSRQLRQMQQDASGGR
                     ADIGGVEQTLRTIGTVNSAEEIGAMEIALVDGRRVRLDELA"
BASE COUNT         1127 a         1658 c         1692 g          986 t
ORIGIN      
        1 agttgcggct ggatatcgac attgaagcgg cgcgaggtct ggaagagtcg cagcaagacg
       61 cggccaaagg aaatttcgtg caggggccgg tcgaagatcg gttcgcaaac cgcgcggatg
      121 gccgattcga attcatcgac gcgggtgtcg gccggggccc agccggcctc gatgtgcgcc
      181 atcgccacac gcttgtagtc gcgctggaag aaggcgagaa aattctgcgc gagatagttc
      241 ttgtcgacgt cggtgagcgt accgacaatc ccgaaatcga gtgcgacgta cttgccgtga
      301 tgcacttcgt cactggcgac aaaaatattg ccggggtgca tgtcggcatg gaagaagccg
      361 tcgcggaaaa cctgcgtgaa gaagatctcg accccggcgc gcgacagtgt cggcagatcg
      421 atcccggcgg cggtcagcgc tgtggtctgg ctgatcggaa tgccgtgcat gcgttccatg
      481 accatcactg tcgtcgtgca gtgatcccaa tacacgcttg gcaccagcag caatttcgaa
      541 cccaggaaat tgcgccgaag ttgtgaacag ttggccgcct cgcgcatgag atcgagttcg
      601 tcgtagagat acttggcgaa ctcggcaacg acctcgcgcg gcttcagccg cttgccgtcg
      661 gaccagacct tctccagcag accggccagc gtgtcgagca atgccagatc gtgagcgatg
      721 atgccgcgca tgttcgggcg caggatcttg accgctactt cgtgcccgcc atcctccggg
      781 tgcaagcggg cgaaatgcac ctgggcgacc gaagcgctgg ccaccggggt ggcattgaat
      841 tcggcgaaga cttcgctcgc tggccggccg taagcttcct cgatttgcgc cagcgccaac
      901 cccggtgaga agggcggcac acgatcctgc agttttgcca gctcgtcggc gatgtccagc
      961 ggcagcaggt cgcggcgcgt cgacatgacc tgtccgaact tgacgaagat cgggcccagc
     1021 gactccagtg ccagcctgag ccgcacggca cgcggcaagg tgttgcgtcg ccagaagttc
     1081 acaatgcgcg acaatgcggc cagtgttcca ctcggctcgt gttcgaaaat catctcgtcg
     1141 atgccgtaac ggctgacgat accgacgatt ttggcgagac ggaaaatgcg cattgggaga
     1201 cttgggcaaa aatgcgagtc taccctgaat cggtaccagc cgattacttc gatacgttca
     1261 gcgcgcgcta acaggctccg gcagcacgat attcacttcc agcacttcgt agttgccctg
     1321 tttttccagc gacaccttga tgtcgtccgg attgaccagg acatatttgg aaatgacggc
     1381 gatcaattcc tgctgcagag ccggcaggaa atcgggcttg gtgttctggc cactgcgctc
     1441 gtgcgcaatg atcagctgca ggcgttcctt ggcaatagtg gctgttttcg gcttgctgcc
     1501 gaagatgaga gagaacaggg acatccttat ttccccccga acattcgctt gaacagaccc
     1561 ggttttgtgt aatcgacaaa gcgtagtggc ggggtttcac caagaaatcg gctgacgaca
     1621 tcgaggtagg cctcggagac gtcgctgcca gagacatgga cggctggaga gccctgattg
     1681 gacgactgca gaatgacctc ggactcggga atgacaccga tgattggcac ccgaaggatc
     1741 tcttgcacat ccttatagga aagcatttcg ccgtcctcga cgcgcttcgg cgagtagcgc
     1801 gtgatgagca ggtgctcctt gaccggctcg tcgcccgaga tggcgcggcg cgatttggcc
     1861 tggatgatac cgagtatgcg gtcggagtca cgcaccgagg acacttccgg attggtgacg
     1921 acaatggctt cgtcggcgaa agtcaatgcc atgacggcgc cacgctcgat tccggccggc
     1981 gaatcgcaga cgacatagtc aaaccccatg tgttcaagtt ccttgagcac tttttcgacg
     2041 ccttcttcag tcagcgcatc cttgtcgcgc gtttgcgaag cgggcagtac gaagaggttg
     2101 tcattgctgc agtgcttgtc cttgatcagc gcctgcgtga gcgtggcttc ctctttgagg
     2161 acgttgacca ggtcataaac cacgcgacgt tcgcagccca tgatcaagtc gaggttacgc
     2221 aggccgacgt caaagtcgat gaccgcggtt ttaaagccac gcagggcgag gccggaagca
     2281 aaactggcgc tggtggtggt tttgcccaca ccacccttgc cggaggttac gacaacgata
     2341 cgagtcaagg atgtttctcc ataaaaaaag gggtagattt tacctgtgga tcggattgct
     2401 tgccgcgccg cagccagcgg ggcgaccacg tggggcgatc aatttgtctt cagcgcctcg
     2461 atcgacaagc ggctatccgt tccgccttgg gtttcgataa gcttaatctg cgccgactgg
     2521 ctggccagtt gatccgggat tccggcctcg aaagtacggt aaatgccggc gattgaaacc
     2581 agttccgctt cgaagcgggt ggtgaagatg cgtgcatccc tggcaccgct ggctcccgcc
     2641 agcgcccggc cacgcaatgg ggcgtaaatg tgaatactgc cgtcggcaat gacttccgca
     2701 ccggcattga cggcggccaa taccaccagg tcgccgcccc gcgcgtagat ctgctgcccc
     2761 gaacgcaacg gacggtcgat aaccaaagtc ggggaagagg ccgggaggac gaccggaggt
     2821 tcactgactg cttcagcaac aacagcggcc tcctgaaccg gttcgtcctg aacggctgcc
     2881 gctggagcag atttctcaat gcggcgtgaa gatcgcttga aagccgtaaa actcggcaag
     2941 ccggcgttgg cagcactttc gtgtagcgca tcgctgccgc cacgaacacc gatgacacgc
     3001 aggccgtgct ggctaaaggt actgcataga gccgcccaat caacagcctc ggaaacctcg
     3061 ccagcgtcga tccggtcaag ttcaaggatg gcggcgtcac gatcaaagaa cgcttcctcg
     3121 ccgaacagct cggtggccgc cccggccaac gctcctgact gcaatgaatg cagcgtgacg
     3181 ctgacaacgg gcagcgtggt gcccttgaat tcgattaacg agcttgtctt gctggtacgt
     3241 gccatggggg ttcgcaaaag tgatgcggca atcctgaagg aagcgaagtt tagcccagtc
     3301 cagagcggaa acgaaagcgg aaacgaaatg cctggcaagg caaagctgaa agtaagtcaa
     3361 ctggagttac aatcctgctg gtcgaacaaa acgcccgcgc ggcagtccaa gcagccgatt
     3421 gcggctacgc accggaaagc ggcgggattt cgctccaggg gccgagccgg gacacggcga
     3481 acgacaatcg gacaaccaca aacgcaccat ggaaagccac cgcggaccga gcgtccggca
     3541 cgaatcatga agcccatcca taccgagaac aacgccggcc tgaggctcgt ccaggccagg
     3601 gtatgccttg cagtcctgct tggtgtcttg ctcgctgctt gcggcaaagc cccaggtgag
     3661 gcggatgcgt cgacgcaacc cgtcaaggcg gcgctgaccg tcagcaccac cgtaccgcag
     3721 cggctcgatt ggccggaaac cctgcgtgcc aacggcaaca tcgccgcctg gcaggaggcc
     3781 gtgatcggcc cggaactgag caattaccgg attaccgagg tcaatgccaa cgttggcgat
     3841 gtcgtcaaga aaggccaggt tctggcgcgc atcgccgccg atacggtcga aaacgaactg
     3901 tccgaaagtc gggccgccgt cgccgaagcc gaggccacgc tggccgaagc ccgcgccaac
     3961 aacgcgcgcg ccaagcagct ccgcgacaag ggtttctaca gcccgcagca gggcatacag
     4021 acgcagacag ttgccgatac cgcactggcg cgcctcaatg ccgccagggc gcgactgcaa
     4081 accgcgcagt tgcggcgctc caaagccgcg gtactgtctc ctgacgacgg aatcatctcg
     4141 gcacgctccg ccaccgtggg gacgctgacc caacccggcc aggaactgtt ccggctgatt
     4201 cgcggtagcc ggctggaatg gcgtgcggaa gtcaccgaag ctgaaatcgc acgcctcaag
     4261 cccggacagg cagcggcctt gagcagcccc accggcgatg tgctcgaagg cgtggtgcgt
     4321 acggtggcac cgaccgtcga cccgcaaacc cgcaccgcgc tggtctatgt cgacctgccg
     4381 gcgaatgcgg cgcaggcgct cagtgccggc atgttcatcc gcggcgaatt cagcctcggc
     4441 cagaacccgg cgctgacctt gccgcaatcg gccgtggtct tgcgcgaagg ctttgcctat
     4501 gtcttcctgc tcgaaaacga caaggttagc cagctcaaga tcgagaccgg ccgccggatc
     4561 ggagatcgca tcgaagtcag cggactcgac ccgcaggccg aagttgtcgc cagcggtgcc
     4621 ggcttcctgg cggatggcga tacggttcac gtcgttgcgg ctcagcccgt cccagtcacg
     4681 cctcgatgaa cgtttcaacc tggtccattc gcaaccccat tccggcggtg ttgctgttcc
     4741 tgatgctcac cctgctcggc ctgatggctt tcaggggaat gaagatccag caattcccgg
     4801 acatcgacct gcccactgtc accgtcacgg cgagcctgcc gggtgcggct ccggcgcaga
     4861 tggagaccga agtcgggcgc aagatcgaga acgccgttgc cacgctgcag ggcgtcaagc
     4921 acatctacac cagggcccag gaaggcacgg tcgttgtctc ggtcgaattc cgtctggaga
     4981 agccgacgca ggaaggcatc gacgacgttc gcgatgccgt ttcgcgaatc cgcgccgatc
     5041 tccccggtga actgaaggac ccggtgattg cccgcgtcaa tctcgccggt gcgccgatcc
     5101 tgacctacac cgtcgcatcg tcccgcatgg acgacgaggc cttgtcgtgg ctggtggaca
     5161 acacgctaac caaggccatg ctcagtgtgc gcggcgtcgg cgccgtcgcc cgtgtcggcg
     5221 gggtcagccg cgagattcgc atcgaactcg atccggcccg gctgctcgca ctgcacgcca
     5281 cggcggccga tgtttcgcgc cagttgcgcc agatgcagca ggatgcctcc ggggggcgcg
     5341 ccgatatcgg cggcgtcgag cagacactgc ggacgattgg cacagtgaat tccgccgagg
     5401 aaatcggcgc catggaaatc gctctggtcg acgggcgccg ggtccgtctt gacgagttgg
     5461 cca
//
LOCUS       DAORPM010000169         5457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4182_length_5457_cov_8.965198, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000169 DAORPM010000000
VERSION     DAORPM010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4182_length_5457_cov_8.965198"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLD53_08625"
     CDS             complement(<1..301)
                     /locus_tag="PLD53_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRC61019.1"
                     /translation="MSESPLYAELAAQSLQLAENGLTRRRRTLDAPCGPLARVDGRDL
                     VSFCSNDYLGLANAPELVEAACAGARVWGVGSGASHLVSDHMRPHDELEHQLAAFT"
     gene            complement(510..2708)
                     /locus_tag="PLD53_08630"
     CDS             complement(510..2708)
                     /locus_tag="PLD53_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRC61020.1"
                     /translation="MKRIAIFCGIITPSMFALAVESDPANIVVTAKSNETPNAIAVAP
                     NTLRAQQAATSDTASLLRDVPGVSLYGAGGTSSLPVIHGLADDRLRIKVDGMDLIASC
                     PNHMNPALSYLDPSNVAKLKVYAGISPVSVGGDSIGGTILAETPTPEFAAAGAGTLLK
                     GEVGAFYRSNGGATGGNISATMATESFNLSYSGSAAKSDNYKAGGDFKTRTDTGRPGH
                     TLPLDEVGSTAYDTQNQSIGVAFKSGQHLIEAKFGYQNVPEQLFPNQRMDMLENTQHR
                     ANLRYVGDFDWGSLEARAYRETVEHYMNFGADKQLAYGTAINGMPMNTKGKTTGGSIK
                     ASINLSLQDLLRIGGEVQHYTLNDWWPPSGTGMMSPNTFININDGERNRTALFGEWEK
                     QLSPQWMTLLGARYELVTTNAGPVHGYNLATFPTVGVPAGMMNQTRDAANFNNADRDK
                     TDNNLDLTALGRYTVNANHDIEFGLARKVRSPNLYERYSWSTANMMAIMNNYAGDGNG
                     YIGDINLKPEKAYAISATFDWHAANREWEFKTTPYYTRVSDYIDAIKTAAFTANQFNV
                     LQFANQSARLYGLDVSGHMPLARTSFGEFGLKGLLSYTNGKNRDTGDDLYNIMPLNAK
                     LMLTQQLGAWDNSAELVMVKAKDQVSDVRNEIQTAGYSLVNLRASYQWRQARIDFGVE
                     NLFDKRYDLPLGGAYVGQGSTMALNAANMPWGIAVPGAGRSLYAGLNFKF"
     gene            complement(2779..3267)
                     /locus_tag="PLD53_08635"
     CDS             complement(2779..3267)
                     /locus_tag="PLD53_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRC61021.1"
                     /translation="MNFSACTTRIAISLAATLISLSAAAQVSVSNPWVRATVPAQKVT
                     GAFMDLTATQDARLVAVKSPGAGTVEVHEMSTDGGVMKMRAMPDGLELPAGRSVELKP
                     GGYHIMLMDLKAQVKEGDRVPMTLIIEYKDGKRQTLDVTAPVKALSTSAAPKGHSNHV
                     AH"
     gene            complement(3300..3917)
                     /locus_tag="PLD53_08640"
     CDS             complement(3300..3917)
                     /locus_tag="PLD53_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRC61022.1"
                     /translation="MTALSAESYGRAPVAATPNASPYFYFSDDRPIYLAGTTGPSWRI
                     RRGVVRLSHPSPDGNTPVFAGIARAGDILGSEIQLFGTYTCDAYPIGEVELEAWLQAD
                     TRSLLRTIAAAERRTVEAISLRAGEAMDRIRRLFVILSGETAQETIPVFIPCLKDMAD
                     ITGLTQETVSRVVSQLRRLGILTRQGRSHGVIVMNALKKLPLQAS"
     gene            complement(4485..>5457)
                     /locus_tag="PLD53_08645"
     CDS             complement(4485..>5457)
                     /locus_tag="PLD53_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRC61023.1"
                     /translation="IQRGLDYSGPALFSVFSGAATGSGELPPYLIAAAAMESRVFPSF
                     TFDPAAGRDWASRFSLDTNPQSALDWPLREFSYEDEKHQSVAQQTPFTLIDFVACDSR
                     YSKHFARVPRARWNGTLTPVGETVAHAERVPLDSVPCLMMVDPENRLQKVIVDEKLIR
                     EARRCRDMWNSLQELGGINNSHADRLLAREKEIRLETVDLAGVAAASAITAVAAVAAA
                     PSAATAPADPPADQGTESEAERSSDEAYIETSRCSTCNECTQINNKMFAYDDNQQAYI
                     ADLGAGTYAQLVEAAESCQVSIIHPGKPRNPNEPGLDELLKRAEMFL"
BASE COUNT         1031 a         1493 c         1733 g         1200 t
ORIGIN      
        1 cggtgaaggc ggcgagctgg tgttcgagtt cgtcgtgcgg acgcatgtga tcgctgacca
       61 gatgcgaagc gccgctgccg acgccccaga ctctggcgcc ggcgcaggct gcttcgacca
      121 gctcgggggc attggccaga ccgaggtagt cgttgctgca gaaactgacc agatcgcggc
      181 catcgacgcg tgccagtggc ccgcacgggg cgtccagggt gcgtcggcgg cgcgtgagtc
      241 cgttttcggc cagttgaagt gattgcgcgg caagttctgc gtagagtggg gattcagaca
      301 tcggtgttca gtgagggtgg cgggtattga gggcattcgg gcattgaatc aagcatcaaa
      361 gcgatggccc tgaagcggtg gacaggttat cagttttgtt gggtgttatt tctgtccaga
      421 ttgggcaagc tcggcaagcc gtagcttgcc gaggctgaag taggccgtat cgagagtctt
      481 tcagtcaagt gttgcgtgca aaagacgacc taaaatttga aattgaggcc tgcatacagg
      541 gagcgtccag cgcctggaac ggcaatgccc cacggcatgt ttgccgcgtt cagcgccatc
      601 gtgctgccct gacccacata ggcaccaccc agcggcaggt cgtaacgttt gtcgaagagg
      661 ttttccacac caaaatcgat gcgcgcttgc cgccactgat agctggcgcg cagattcacc
      721 aggctatagc cggcggtctg gatttcattg cgcacgtccg atacctgatc cttggctttc
      781 accatcacca gttcggcgct gttatcccac gcgccgagtt gttgcgtgag catcagcttg
      841 gcattgagcg gcatgatgtt gtacagatca tcgccggtat cgcggttctt gccattggtg
      901 tagctcagca gacccttcaa gccgaactct ccaaagctgg ttctggctag cggcatgtgg
      961 cctgatacat caaggccgta cagccgcgct gactgattgg caaattggag tacgttgaac
     1021 tggttagccg tgaaggcagc cgtcttaatg gcgtcaatat agtcactgac tcgcgtgtaa
     1081 tacggtgtgg tcttgaattc ccattcgcgg tttgcggcat gccaatcaaa ggtggcgctg
     1141 atggcgtaag ctttttccgg tttgaggttg atgtcgccga tgtagccgtt gccgtcgccg
     1201 gcgtagttgt tcatgatcgc catcatgtta gccgtcgacc acgagtagcg ctcatacagg
     1261 ttcggcgagc gaacctttcg tgcaaggccg aattcgatgt cgtggttggc gttgacggtg
     1321 tagcgaccga gcgcggtgag atccaggtta ttgtcggttt tgtcgcgatc ggcattgttg
     1381 aaattggcgg cgtcccgggt ctggttcatc attcccgcag gtacgcctac ggtcgggaat
     1441 gtcgccagat tgtagccatg taccggaccc gcattggtcg tgacgagctc gtagcgcgcg
     1501 ccgagcagtg tcatccattg ggggctcagc tgcttttccc attcaccgaa gagcgcggtg
     1561 cggttgcgtt cgccatcgtt gatgtttatg aacgtattgg gagacatcat gccggtgcct
     1621 gagggtggcc accagtcatt gagcgtgtag tgctgcactt cgccgccaat gcgcaggagg
     1681 tcctgcaaac tcaagttgat actggctttg atgctgcccc cggtcgtctt gcctttggtg
     1741 ttcatcggca tgccattgat ggctgtccca taggccagtt gcttgtcggc accgaaattc
     1801 atgtaatgct caaccgtttc ccgatacgca cgggcttcca gcgaacccca gtcgaagtca
     1861 ccgacatagc gaaggttggc gcgatgctgg gtgttttcga gcatatccat gcgttggttg
     1921 gggaagagtt gctcgggcac gttctgatag ccgaatttgg cttcgatgag gtgctggccg
     1981 ctcttgaaag ccacgccgat ggattggttc tgcgtatcgt aggctgtgga gcccacctca
     2041 tccaagggca gggtgtggcc gggccggccc gtgtcggtgc gggtcttgaa gtcgccaccc
     2101 gctttgtagt tgtccgattt ggctgcggag cccgagtagc tcaagttgaa ggattcggtt
     2161 gccatggtcg cagatatgtt gccgccggtc gcgccgccat tgctacggta aaaggctccc
     2221 acttcgccct tgagcaaagt acctgcgcct gcggcagcga attcaggggt gggtgtttcc
     2281 gcaagaatgg tgccaccgat gctgtcgcca ccgacgctga ccggtgaaat ccctgcatag
     2341 actttaagct tggcaacatt gctcgggtcg aggtaagaca gtgccgggtt catatggttg
     2401 gggcaggagg cgatcaggtc catgccatcg accttgatac gaagccggtc gtcagccagg
     2461 ccatggatga cgggaagact cgatgtgcca cccgcgccgt acaggctgac gccgggcaca
     2521 tccctgagca ggctggccgt gtcgctggtc gctgcctgct gggcgcgcag cgtgttggga
     2581 gcgacggcta tcgcattggg ggtttcatta ctcttggcgg tgacaacgat gttcgccggg
     2641 tcgctctcta cggccaaagc gaacatagac ggcgtgatga tgccgcagaa aatagcaata
     2701 cgtttcattg ttaaacctat cgaaagatgt cattggatcg tgcgctaccg acactcctgg
     2761 caagcgcact tctcgtattc agtgcgcaac gtgattgctg tggcccttcg gtgcggcgct
     2821 ggtcgacagt gccttgaccg gagcggtgac atcgagggtc tggcgcttgc catccttgta
     2881 ttcgatgatc agggtcattg gcactcggtc gccttccttc acttgagcct tcagatccat
     2941 gagcatgatg tggtagccgc ccggtttgag ttccaccgat ctgccggcag gcagttcgag
     3001 gccgtcaggc atcgctcgca tcttcatgac gccgccatca gtgctcattt catgcacttc
     3061 gaccgttccg gcgcccggtg atttgaccgc gaccagacgg gcatcctgtg tggcggtcag
     3121 atccatgaat gcgccggtga ctttttgtgc gggcacggta gcgcgcaccc aggggttact
     3181 gacgctgacc tgggcggcgg ccgatagtga aatgagcgtg gcggccaggg aaatggcaat
     3241 acgggtcgta caagcagaga agttcatagg taagtccttg attaggaatg tttgaggcat
     3301 taagaagcct gaagaggcag ttttttgagt gcgttcatta cgatgacacc gtggctgcga
     3361 ccttgtcggg tgaggatgcc caggcggcgc agttggctga cgactcggga tacggtttcc
     3421 tgcgtcaggc cggtgatgtc cgccatgtcc ttcaggcacg ggataaacac cgggatagtc
     3481 tcctgcgccg tttcccccga aagaatgacg aacaagcgcc ggatgcgatc catggcttcg
     3541 ccggctcgca gggaaatcgc ctccaccgtg cggcgttccg cagcggcaat tgtgcgcagc
     3601 aggcttcggg tgtcggcttg gagccaggct tccagttcaa cctctccgat gggataggcg
     3661 tcacaggtgt aggtgccaaa gagctgaatc tcgctgccga gaatatcgcc ggctcttgca
     3721 atgccggcaa agacgggggt attgccgtca ggagatgggt gactcaggcg tacgacgccg
     3781 cgccgaatgc gccaactggg tccggtcgta ccggccaggt agatcggccg atcatcgctg
     3841 aaatagaaat agggtgatgc attgggcgta gcagcgacag gcgcgcgccc gtagctttcc
     3901 gcagaaagtg ccgtcatggt cttctgtcct tccgagtaat gacaaagctc agacgccggc
     3961 gtcagccggt cagtctgtca tcgtgatgac gcaggatcag aagtgcggtg gtgcgtggga
     4021 aggcggcagg cggtatcgac ccgcaggcag atttacgcca gaatcgttcc atgtgcgggc
     4081 aacgaaagcc agtgcctgtg agacggcgcc aggaccagcc agcgccaaca acgtgggagt
     4141 gctgccacag taggtacagc agctatggtc gctgtgatta tcgtgtgacg ggacgctgtc
     4201 gcccggcccg gccgttggca attgaaccgt tatcaagcca ttgacggtac agatctggcc
     4261 tacgaccgaa ccgtctgccc ctgccttggg catgatgccc aaggcagccc agagctgaag
     4321 ggcaagcgcc agcaggccaa gaaccaggaa ggtccggccg cgttttcgga agtgccagaa
     4381 taaggcggaa gagtctgtaa tcacgtgcgc ggaattgtat agcacgcgag agatatccac
     4441 caagcactgg ccgcgaactg agcgcgggca gtgtgctgaa ttgattacag aaacatctcc
     4501 gcccgcttga gcagttcatc cagccccggt tcgttgggat tgcgcggctt gccggggtga
     4561 atgatcgaca cctggcaact ctcggcggct tcgaccaact gggcgtaggt tccggcgccg
     4621 agatcggcga tgtaagcctg ctggttgtcg tcataggcga acatcttgtt gttgatctgc
     4681 gtacattcgt tgcacgtgga gcagcgcgat gtttcgatgt aagcctcgtc ggatgatcgt
     4741 tccgcttcac tttccgttcc ctgatcggct ggtgggtcag cgggggctgt cgccgcagac
     4801 ggcgcggcgg caacggcggc gacggctgtg atcgcgcttg cagccgctac gccagccaga
     4861 tcgacggtct ccagccggat ttccttctca cgcgccagca acctgtcggc atgcgaatta
     4921 ttgattccac cgagttcctg caggctgttc cacatgtcgc ggcaacgacg tgcttcgcga
     4981 atcagctttt catcgacgat gactttctgc aacctgtttt ccgggtccac catcatcagg
     5041 cagggaacgc tgtcaagcgg cacgcgctct gcgtgcgcca ccgtctcgcc gaccggcgtc
     5101 agggtgccgt tccagcgcgc tctcggaacc cgcgcgaagt gtttgctgta gcgcgagtcg
     5161 caggcgacaa agtcaatcag cgtgaacggc gtttgctgcg ctaccgactg gtgtttttcg
     5221 tcttcgtaag aaaattcgcg cagcggccag tcaagcgccg actgcgggtt ggtatccagc
     5281 gagaagcgcg aagcccagtc ccgacccgcc gctgggtcaa aggtgaagct cggaaaaacg
     5341 cgtgactcca ttgccgctgc agcgattagg tagggcggta gttcgccgct gcccgtggct
     5401 gcacccgaaa agacgctgaa cagggccggg ccggaatagt cgagcccgcg ctggatc
//
LOCUS       DAORPM010000170         5420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4229_length_5420_cov_6.191426, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000170 DAORPM010000000
VERSION     DAORPM010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4229_length_5420_cov_6.191426"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4832)
                     /locus_tag="PLD53_08650"
     CDS             complement(<1..4832)
                     /locus_tag="PLD53_08650"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRC61024.1"
                     /translation="MPSMLPILFSRLRLLGLAAVMLLCGVASAAGIRQFSPQGQVDQQ
                     IRATAVFTSDMVPLGRSGAPSPFSIDCGEVKGSGRWGDARSWSYTLVRPLQPGERCDF
                     RLRPGLKAASGEVVAGESSYAFFAPGPWPRSLTPRPGGAIEEDQAFVIDASGALKTES
                     VERNVWCEADGVGNRIPVRIVPETTRNEILATLHRGMQGGTLIITCAERLPAGAKMRL
                     VWGKGVEAENAGKPGAKSSRDESFHYVVRQPFRASFSCEREKPGAPCSPLSDLRIEFS
                     VPVDAKQAAGARLISPEGTRAPQAQNQEGYRENNVRDLVFKRPLPQNAELTIELPADI
                     KDEAGRSLSNAASFPLKLRTGSLPPLAKFPGAFGIVELKEGGIVPVTLRNVEAGLPVA
                     NLKMPSEKAHRFSTERLTDDAEVIAAIRALARFEEQSRLVKIGEGKEQRDYEDPYYAR
                     ELSFLKGKAGVVSGELPSPGGSAELEVIGIPLQKPGYHIVEIESRLLGAALLASPAPM
                     YVRTSVLVTNLAVHLKRGKDNALVWVTSLDQGKPVGGAEVRVSNCDGKPLWQGRTDNE
                     GRALVDVALPENGCEDDGFIFASARLGADYSFVRSDWNEGIEPWRFGVETWGEFETRK
                     IHTILDRSLLRAGQTVSMKHIARARTSRSFAYPDTQSLPTELVIRHEGSGDEFRQPLS
                     WDSRGVATSQWKIPPSVKRGNYQIELRNPANGEGGGVTTGTFGVSDFRLPVFSGSVQG
                     VSGRLVAPKEVPLALGLAFLNGGAAKGAKVEVSATLRPRWPEYKGYDGFTFLVGFDEQ
                     GRSAFAVDDGREREKLLSNRQPVTLDQVGAGKVVIPLQAKVKGPSELYAEMTFADPNG
                     EIQTLHGSVELSPSGVVAGIHVKDWASVKEDGSVEVVVLDTQGQPAKDAPVKVSAKRR
                     IDYSHRKRIVGGFYAYENHTEFSDLGAVCQGRTDTRGKFLCQPRTTDPGNIYLLVETS
                     DKDGNIALAGTSYWVSGIGDLWFTAGNQDRIDVIPEKRSYAPGEIARLQVRTPFREAT
                     ALVSVEAGGIIETQVMPLSRFKPVIELPVKGEWGPNVFVSVLLVRGRVEPLKWYSLFQ
                     WGWREPVAWFREWWNPLQPTAMVDLAKPAFKLGLAAIEVGVDAFKLKVDVTPEKQDYQ
                     IRDQALVKIRVTQPDGKPVPAGTEVAFAAVDQALLELRPNESWKLLDAMLQKRAYQVQ
                     TATAQSQVIGKRHFGKKAVPPGGGGGRAPARELFDTLLLWNPRVVLDATGSALVSVPL
                     NDSLTEFRLVVVADAGVGLFGTGSASLRTRQDLQLISGLPPLVREKDRFSALLTLRNG
                     TARAMAVRVSARQGDRALEGREVKLDAESAAELVWPGEAPENESMLVWEFEAVETGEA
                     DKKDGVGKDRLRITQQIAPAVPIAVQQASFARVEGRLEVPAALPAGAVSGKRGPLGGI
                     EVGLSARLSTPPPGLRRFFEDYPFVCLEQKTSVAIGLRDAARWQQVIDALPTYLDANG
                     LARYFPDGGGQRGNGGSPALTAYLLDASIAAGFAIPPELAQRMEGGLAAFAEGRIKPE
                     HWSPQSDLTVRKLAAIEALTRRGGSSYSPLSAISSLEIEPLRLPTSALIDWYLIVKRL
                     P"
     gene            complement(4945..>5420)
                     /locus_tag="PLD53_08655"
     CDS             complement(4945..>5420)
                     /locus_tag="PLD53_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC61025.1"
                     /translation="VETHRAVRLRHVLKTPTLHNNRIWALVDKRGFLREMSPAFIVYL
                     RENWPGWNGNKLPEHLLSSIQSGQPVRVASVRFEVTPCGQFHYILGKPDGALEQLSAR
                     EREIATRYARGDTYSAIAASLSLSPATVRNHIAHCFRKLAVSNKLELARRLDGKP"
BASE COUNT         1021 a         1792 c         1626 g          981 t
ORIGIN      
        1 ggcagacgtt tgacgatcaa ataccagtcg atcagcgctg acgttggaag acgcagaggc
       61 tcgatttcca gactggagat ggccgagaga ggcgagtatg acgatccgcc gcgccgggtc
      121 agcgcctcga tcgccgcgag tttgcgcacc gtcagatcgc tttgcggcga ccaatgctcg
      181 ggcttgatgc gtccctcggc aaacgccgcc aggccgccct ccatgcgctg cgccagctcc
      241 ggcggaatgg cgaatccagc cgcgatgctg gcatccagca ggtaggcggt cagcgccggg
      301 ctaccgccat tgcctcgctg tccaccacca tcgggaaaat aacgggccag cccattggca
      361 tcaagatagg taggcagggc atcgatcacc tgctgccagc gcgcggcgtc gcgcagtccg
      421 atggcgaccg atgtcttctg ctccagacag acaaacgggt agtcctcgaa gaagcgcctc
      481 aaacccggcg gcggcgtgga caggcgagcc gagagaccga cctcgatgcc gccaagcggg
      541 cctctctttc ccgaaaccgc accggccggt agtgcggcgg ggacttccag cctgccttcg
      601 acgcgggcga agcttgcctg ttgcacggcg atcggcaccg ccggggcgat ctgctgcgtg
      661 atgcgaaggc gatccttgcc gacgccgtcc ttcttgtcgg cctcacccgt ctcaacggcc
      721 tcgaactccc agacgagcat gctttcgttc tccggtgctt cacccggcca gaccagctcg
      781 gcagcacttt ctgcgtcgag tttcacctcc ctgccctcaa gcgcgcggtc accctgccgg
      841 gccgagaccc tcaccgccat cgcccgggcc gtgccgttgc gcagggtcag caatgcgctg
      901 aagcggtctt tctcgcgcac cagtggcggc aagccggaaa tgagttgcag atcctgccgg
      961 gtgcgcaggc ttgcactgcc ggtgccgaac aggccgacgc ctgcatcggc caccactacc
     1021 agcctgaact cggtcagcga atcgttgagc ggcacgctga ccaaagcgct gccagtggcg
     1081 tcgagcacca cgcgcgggtt ccacaacaac agcgtatcga acagttcgcg cgccggcgca
     1141 cgaccaccgc cgccgcccgg cggtacggcc ttcttgccga aatgccgctt gccgatgacc
     1201 tgcgattgcg ccgtcgcggt ttgcacttga taggcccgct tctgcagcat ggcatcgagc
     1261 agtttccagc tctcgttcgg gcgcagttcg agcaaagcct gatcgaccgc ggcaaaggcc
     1321 acctcggttc ccgccggaac cggcttgccg tccggctgag tgactctgat ctttaccagc
     1381 gcctgatcac gaatctggta gtcctgcttc tcaggcgtaa cgtcgacctt cagcttgaac
     1441 gcatcgacac ccacttcgat cgccgccagc ccgagtttga aagccggttt ggccaggtcg
     1501 accatggctg tcggctgcag gggattccac cattccctga accaggcgac cggctcgcgc
     1561 cagccccact ggaaaagcga ataccacttg agcggctcga cgcggccgcg cacaagcagc
     1621 accgagacga acacgttcgg cccccactcg cccttgaccg gcagttcgat gaccggcttg
     1681 aagcgtgaca gcggcatgac ctgcgtttcg atgatgccgc cggcttcgac agaaaccagc
     1741 gccgtcgctt cgcggaacgg cgtgcgcacc tgcaagcgcg cgatttcgcc cggcgcgtag
     1801 ctgcgctttt cgggaatcac gtcgatgcga tcctggttgc cggcagtgaa ccacagatcg
     1861 ccgatacccg agacccagta gctggtcccg gccagggcaa tgttcccgtc cttgtcgctg
     1921 gtttcgacga gcaggtagat attgccggga tcggtggtgc gcggctggca gaggaacttg
     1981 ccccgcgtat cggtacgccc ctggcatacg gcgccaaggt cgctgaattc ggtgtgattc
     2041 tcataggcgt agaaaccgcc gacgatacgc ttgcgatgcg agtaatcgat gcgccgcttg
     2101 gcgctaacct tgaccggcgc gtccttggcc ggctggccct gggtatcgag cacgacgacc
     2161 tcgaccgaac cgtcttcctt gaccgacgcc cagtccttga catggattcc cgcgacgacg
     2221 ccggacggtg agagttcgac cgaaccgtgc agggtttgaa tctcgccgtt cggatcggca
     2281 aacgtcatct cggcgtagag ttcgctcggc cctttgacct tggcttgcag cggaatgacg
     2341 accttgccgg cgccaacctg atcgagcgtc actggctggc gattgctcag cagtttctcg
     2401 cgctcacggc cgtcatcgac ggcgaaggcc gaacggccct gctcgtcaaa accaacaaga
     2461 aaggtgaaac cgtcataacc cttgtactct ggccagcgcg gacgcaaggt tgccgacacc
     2521 tcgaccttgg cacccttggc cgcacctccg ttgaggaagg cgagcccgag cgccagcggc
     2581 acttccttgg gcgcgaccag acgccccgat acgccctgca cgctgcctga aaaaaccggc
     2641 aggcggaaat cggaaacacc gaaggtgccg gtcgtgacgc caccgccctc gccgttggcc
     2701 ggattgcgca gctcgatctg gtagttgccg cgcttgaccg aaggcggaat cttccactga
     2761 ctggtggcca cgccccggct gtcccaactc agcggctggc ggaattcgtc gccgctgcct
     2821 tcatggcgga tcaccagttc ggtaggcagc gactgcgtgt ccggataggc aaagctgcgg
     2881 ctggtgcgcg cgcgcgcgat atgcttcatc gatacggtct gcccggcgcg cagcaggctg
     2941 cgatccagga tcgtgtggat cttgcgtgtt tcgaactcac cccaggtttc gacgccaaag
     3001 cgccagggct cgatgccttc gttccagtcc gagcggacga agctgtagtc tgcgccaagg
     3061 cgcgcactgg caaaaatgaa gccgtcatcc tcgcagccat tctcaggcaa ggcgacatcg
     3121 accagcgccc gcccttcgtt gtcggttcgc ccctgccaca gcggctttcc gtcgcagttc
     3181 gaaacacgca cttcggcgcc gcccaccggc ttcccttgat cgagcgaagt cacccagacc
     3241 aacgcattgt ccttgccgcg cttgagatgc accgccaggt tggtgaccag caccgaggtc
     3301 cgcacataca ttggcgcggg cgaggcgagc agcgcggcgc cgagcaggcg gctttctatc
     3361 tcaacgatgt ggtaacccgg cttttgcagc ggtatcccga tcacctccaa ctctgcactg
     3421 ccaccggggc tgggcagttc accgctgaca accccggctt tgcctttaag gaaagacaat
     3481 tcgcgcgcgt aatagggatc ctcgtagtcg cgctgctcct tgccctcacc gatcttgacc
     3541 agacgcgact gttcctcgaa acgcgccagc gccctgatcg cggcaatgac ttcggcatca
     3601 tcggtaaggc gctcggtgga aaagcgatgt gccttttccg agggcatttt cagattggcc
     3661 accggcaaac cggcttcgac gttgcgcaac gtcaccggaa cgatgccgcc ctctttcaat
     3721 tcgacgatgc cgaacgcacc gggaaattta gccaaaggcg gcaggctccc ggtacgcagc
     3781 ttgagcggaa agctggcggc attgctcagg ctgcgccccg cctcgtcctt gatatcggcg
     3841 ggcaactcga tggtcagctc ggcattctgc ggtaaaggac gcttgaagac cagatcacga
     3901 acgttattct cgcggtagcc ttcctgattc tgcgcttgcg gagcgcgcgt gccttccggc
     3961 gaaatcaacc gggcgccggc cgcctgtttg gcatcgaccg gcaccgaaaa ctcgatacgc
     4021 agatcggata gcggcgaaca tggtgcaccg ggcttttcgc gttcgcaact gaagctggcg
     4081 cggaacggct ggcggacgac atagtggaaa ctctcgtcgc ggctggattt tgcgccgggt
     4141 ttcccggcgt tctcggcttc aacccccttg ccccatacca aacgcatttt cgctcccgcc
     4201 ggcaatcgct cggcgcaagt gataatcaac gttccccctt gcatgccgcg atgcagcgtg
     4261 gcgagaattt cgttgcgcgt tgtctcgggc acgatacgca ccgggatgcg gttgccgacg
     4321 ccatcggcct cacaccagac attgcgttca accgactcgg tcttcagcgc gccactggcg
     4381 tcgatcacga aggcctggtc ttcctcgatc gccccgcctg ggcgcggcgt cagcgaacgc
     4441 ggccaggggc cgggcgcaaa aaaggcgtag ctcgattctc cggcgacaac ctcgccactg
     4501 gccgccttga ggccgggcct gagcctgaag tcgcaacgct cacccggctg caatgggcgc
     4561 accagcgtat aggaccagct gcgcgcatcg ccccagcgtc cactgccttt gacttcgccg
     4621 caatcaatac tgaacggcga tggcgcgccg gaacgaccca gcggaaccat gtcagacgta
     4681 aacaccgctg tcgcccgaat ctgctgatcg acctggccct ggggagaaaa ctgacgtatc
     4741 ccggcggcac tggcgacccc gcagagcagc atgaccgcag ccaagccaag caaacgcaaa
     4801 cgcgaaaaga gtattggcaa catggacggc atgacgaccc cggcatgaca actggcagac
     4861 cgcccgatta tagaccaggc aacacgctgc cctgcatgca atcttccgta ccacttcctt
     4921 gatgggaaac agcagcgttg aacgtcaggg cttgccgtca agtcgacgtg cgagttcaag
     4981 cttgttgctc accgccagct tgcggaagca atgggcaatg tggttgcgca ccgttgccgg
     5041 cgacaaggag aggctggcgg cgattgcaga ataggtgtcg ccccgggcat aacgggtggc
     5101 aatctcgcgt tcgcgcgcag acagttgttc gagcgcgccg tcaggcttgc cgaggatgta
     5161 atggaactgc ccgcaaggcg tcacttcaaa gcggacagac gccaccctga ccggttgccc
     5221 gctctggatt gaagacaaca ggtgctccgg tagcttgttt ccattccagc ccggccagtt
     5281 ttcgcgcagg tagacgatga aagccggtga catttcacgc agaaaaccac gcttgtcgac
     5341 cagcgcccaa attcggttgt tatgcagtgt cggtgttttg aggacgtgac gcagccgcac
     5401 cgcgcgatgc gtttcaacaa
//
LOCUS       DAORPM010000171         5409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4248_length_5409_cov_10.533806, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000171 DAORPM010000000
VERSION     DAORPM010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4248_length_5409_cov_10.533806"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLD53_08660"
     CDS             <1..212
                     /locus_tag="PLD53_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61026.1"
                     /translation="AQHTGRRYGALFSDEADGPLDPERKRMFMAMKREVLRLGGYQRE
                     FFVSQTPELTAMADAVIDMDLQGRL"
     gene            782..2446
                     /locus_tag="PLD53_08665"
     CDS             782..2446
                     /locus_tag="PLD53_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HRC61027.1"
                     /translation="MTATVVASVDANPPSEQFQAVSLWTFVHLKTPRFTSEAALDVVR
                     HLMPTADEDPKHLAKRLRKSLLEHGIALKHVNSLDAAARLLGHADWHAANRAQPKTTL
                     KLTPMADAAEELFEDWRQLAPRLCAWCDAWLRGKGGKVFEVRFGPGYVMVSIPTPKEG
                     GPAGSMDELPLLMVNPVGDAEHWLQEAPAAFETLRRHLEESGQAVLDGVAVLQLCNRY
                     SREVLDRLPSIPQPVRPTDAGNSELVLLREDDELMPGSGYEIARGDELTCWAQLHLAM
                     KDHKSEGITLDDGAWRIGGGRYVWQLSTIHPKDFVPGLVITMLSETDSEKLLRRYKLV
                     QRVLSQNFKHHEVTKRLQYLSGPSDIYRVDLHKLLLALNDAGLTWEGFCQEVEMQQAM
                     VPELPVGFAMTIIERLKPKDPNLFFALPSRAELARADDDSLLRTLLPRIDIVRYRIVR
                     GVSDEVKQTVREAIDEFGTSIRMQALTAAGQLTDPNDPLPYLVYAGDGEELRLKLESE
                     GLVMYAGVMPHLFPTEGVVEKLPNMWSYAFGHSLFLDVDFAEGGAQ"
     gene            2443..3426
                     /locus_tag="PLD53_08670"
     CDS             2443..3426
                     /locus_tag="PLD53_08670"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HRC61028.1"
                     /translation="MNATIENLRIFLEERDIGGFLAAYETMVPGADVLSSQLTKITQQ
                     IFPTVLHEKLTRGALVAGDTVRLWRLVRCGRLLVVDNDLLEGINALLDAAWREVEGPD
                     APLPTKLLTYREAPEGSVQGRRSARPAAAAATSQGDVVPMRRIVMASAFTLGPVHLSD
                     LMTFRKSVCASSQEREFLQAVRQFFPSLRAYPNVPLRNFMDVDGFVLRLSDRHRRYSW
                     AAQVDVLLCTEDEDPVACFELDSALHDDDDVRERDQLKDELFALAAIPLLRVRADDTA
                     NVRAEDFYDLLVADSETLDRLRPRRLRPRRNHDMLVPAEVQVRRSAAKAYC"
     gene            3438..4781
                     /locus_tag="PLD53_08675"
     CDS             3438..4781
                     /locus_tag="PLD53_08675"
                     /inference="COORDINATES: protein motif:HMM:NF039631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6035 family protein"
                     /protein_id="HRC61029.1"
                     /translation="MGPTVLDERGAFAVENPEVAEVMEIASGDHLDHGEVIGTDYEKA
                     IQLRMALRTDIKRGTPHYACSLCGVPVYLVSRAEERRFFFRHTLEDGRCLARTRGELS
                     QEEIDARRYNGVKESARHLQMKEWVAQCLAADPRFTDVATEKRWSGTLTAEWRKPDVR
                     AIYRGIPVVFEIQLSTTYVNVIAERREFYLQEGGLLIWIFAHFDGGARRLTQDDVFFN
                     NNRNAFVVTQATRDASVQRGRFMLDCIWAEPTLMGNSDLQRRVVGFDDLTLERESQRA
                     YYFDFDGARDALQEQARERERQRLAEVREKFETWWLDYETSDTPDYRGWAELRRALAA
                     ERVELPQYPNHVPRTLLNALYSAKHGRVIGWRFKTFIEVAHRVEPAHRRYLHYFRRAL
                     AAYDRADQLRAEDKSGKWALKVKHYKAQMQINDPAYTPDTSDMKLVVLLFPEIFV"
     gene            complement(4858..5196)
                     /locus_tag="PLD53_08680"
     CDS             complement(4858..5196)
                     /locus_tag="PLD53_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61030.1"
                     /translation="MSRHHIRLMHQGQPVLVVAGYDRPLRELFLQVFLCQESGPAGDD
                     DIVYDSLHEPALDWTVIGTLTDKLTALGICVPEKLIEAVYLDQRFTTGNVVVWHHADQ
                     APEVLPTNRA"
     gene            complement(5193..>5409)
                     /locus_tag="PLD53_08685"
     CDS             complement(5193..>5409)
                     /locus_tag="PLD53_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61031.1"
                     /translation="LADDELSVNAYPTDCGGILYVGMPCHRIPTEADLAAIFEVAQLA
                     GVVWLNFDSEAAVIDGLPIFEMPDPAS"
BASE COUNT         1085 a         1513 c         1692 g         1119 t
ORIGIN      
        1 tcgcccagca cacggggcga cgctacggtg cactgttctc ggacgaggcc gatggaccgt
       61 tggaccccga gcgcaagcgc atgttcatgg cgatgaagcg cgaggtgctg cggctggggg
      121 ggtatcagcg ggagttcttc gtgtcccaga cgccggaact gaccgccatg gcggatgcgg
      181 tgattgatat ggatcttcag ggccgcttgt aggattgcca tggcttctct ggcgccccgc
      241 cgctaaaatg tctgcatgtc gaaaatcccc gaatccacgc ctgcgttgaa caggctgcgt
      301 gcggcggccg gcttaatccc tcttattgag gatggcctcc gccactcgaa aatcaccgcc
      361 gagaaggctt ctttaatgtc ggagttttgc agctgggcgg cccagcaagg aacagaaggc
      421 ggcccggagg ccttacgcct tggcgatgac atcaaagcgg gcttggaacg gcttaaaacg
      481 ctactggcat agcggtggcc gggcatgtcc ggccaccgtc actatcgttt ggccccgttg
      541 tgagagctgg attgccgact cacggctcgg gcgttgacgg aatttgtttc attgtcctat
      601 catgtactca cggttcaagc cgaatcgtgg gcaagttggc gcccgtcagt gcacgcaccg
      661 gagtctttcc ggcacggcaa agccagttga atacccgcaa gtaacctccc ggctaggtga
      721 actcctggaa agccggacaa cgcgtgccgt cctgaagtgc tttccgatat ggagctcatc
      781 aatgacagcg accgttgtgg catctgtgga tgccaatccc ccctctgaac agtttcaggc
      841 cgtctcactc tggactttcg tccacctgaa aacacctcgc tttacttcgg aagcagcact
      901 cgatgtggtt cggcacctga tgccaaccgc tgacgaggat cccaagcatc tcgcgaaacg
      961 cctgcgtaaa tccctgctag agcacggcat cgccctcaag cacgtcaatt cgctcgatgc
     1021 ggcggcgcgc ctgcttgggc acgccgattg gcacgcggca aatcgcgcgc agccgaagac
     1081 gaccctcaag ctgacgccga tggccgatgc cgcggaagag ttgttcgaag actggcgcca
     1141 acttgcacca cgtctgtgtg catggtgcga tgcatggctt cggggaaaag gtggcaaggt
     1201 ctttgaggtg cgtttcggcc ccggctatgt catggtcagc attcctacac ccaaggaggg
     1261 tgggccggca ggaagcatgg acgagctgcc gctgcttatg gtcaatcccg tcggggatgc
     1321 cgaacattgg ttgcaggaag ccccagcggc attcgaaact ctgcgccgtc acttggaaga
     1381 gtccggacag gcagttctgg acggtgttgc cgtgctgcag ttatgcaatc gctacagtcg
     1441 ggaagtattg gaccgtttgc cgtcgatacc gcagccggtg cgaccaacgg atgccggcaa
     1501 ttcggaattg gtcttgctgc gcgaggacga tgagctcatg ccggggtcag gctacgagat
     1561 tgcccgtggc gacgagctga catgctgggc acagctccat ctggcgatga aggaccacaa
     1621 gtccgaggga attactttgg atgacggggc ctggcggatc ggtgggggcc gctatgtatg
     1681 gcagttatcg acaatccatc ccaaggactt tgtccccggg ctggtgatca cgatgctgag
     1741 cgagaccgat tcggagaagc tgctgcgtcg ctacaagctt gtacagcggg tactttcgca
     1801 aaacttcaag catcatgagg tgaccaagcg cttacagtac ttgagtggcc cgtcggatat
     1861 ctaccgtgtt gatttgcaca aactgctatt ggcattgaac gacgcgggtc tgacctggga
     1921 gggcttttgc caggaggtcg agatgcagca ggcaatggtc cccgagctgc ccgttggttt
     1981 cgcgatgacc atcatcgaac ggctcaagcc gaaggacccg aatctcttct tcgcactgcc
     2041 gagccgtgca gagctggccc gagccgatga cgattccctg ctgcggacct tgttgccccg
     2101 cattgacatc gtgcggtacc ggatcgtgcg tggcgtgtcc gatgaagtta agcagaccgt
     2161 ccgcgaagcg attgacgagt tcggtacgtc gatccgtatg caggcgctga cggctgcagg
     2221 gcagctgacc gatccgaacg atccgctgcc gtatctggtc tatgccggcg atggcgaaga
     2281 actccgacta aagctcgaat ctgaagggct cgtaatgtac gcgggagtca tgccgcattt
     2341 gtttccaacc gaaggcgtgg tcgagaaact gcccaacatg tggtcttacg ccttcggaca
     2401 tagcctattt ctggacgtcg acttcgcgga aggtggtgcg caatgaatgc caccatcgag
     2461 aacctgcgga tattcctgga agagcgcgac atcggcggct tcctcgctgc ctacgaaacg
     2521 atggtgccgg gtgccgacgt ccttagcagc cagctgacca agattacgca gcagatcttt
     2581 ccgacggtgc tccacgagaa actcacaagg ggggctttgg ttgcaggcga cacggtccgc
     2641 ctttggcgtc ttgttcgttg cggtaggctg ctggtagtcg acaacgacct gcttgaaggc
     2701 atcaatgcac tgcttgatgc cgcatggcgc gaggtcgagg gaccggatgc cccgctcccc
     2761 acgaagttgt tgacctatcg cgaggcgccg gagggatcag tacagggccg acgttctgca
     2821 aggccagcag cagctgccgc gaccagccag ggtgatgtgg tgcccatgcg ccgcatcgtg
     2881 atggccagcg catttactct cgggccagtg catttgagcg atcttatgac cttccgtaag
     2941 agcgtttgcg ccagctccca ggaacgcgaa tttttacagg cggtgcggca attcttcccc
     3001 agcctgcgcg cctaccccaa cgtgccgcta cgtaatttca tggatgtcga tggcttcgtc
     3061 cttcgtcttt cagatcgaca ccggcgctat tcgtgggcgg cacaagtcga tgttctgtta
     3121 tgcaccgagg acgaagatcc cgtggcttgt ttcgagctgg actcagctct gcatgatgac
     3181 gatgatgtcc gcgaacgcga ccaattgaag gacgaattgt tcgcattggc tgcgattccg
     3241 ttgctccgcg ttcgggctga cgatacggcg aacgtgcgcg ctgaggactt ctacgacctg
     3301 ctggtcgccg atagcgagac cctggacagg ttgcgtcccc gtcgcctgcg accgcgaagg
     3361 aaccatgaca tgctggttcc tgccgaggta caggtgcgcc gatctgcagc caaggcctat
     3421 tgttgagagg gctagtcgtg ggacctaccg tactggacga acgtggtgct tttgctgtcg
     3481 agaatcccga ggttgccgag gtaatggaga tcgcctcggg ggatcatctc gatcatggcg
     3541 aagtcatcgg caccgattac gaaaaggcga tccaattgcg gatggcgctc cgcaccgaca
     3601 tcaagcgagg cacgccccac tatgcctgct cattgtgtgg ggtgcctgtc tacctagtgt
     3661 cgcgcgccga agagcggcgt ttcttcttcc ggcatacgct ggaagatggg cgttgcttgg
     3721 cgcgcacgcg gggtgagcta tcgcaggagg agattgatgc aaggcgttac aacggcgtca
     3781 aggaaagcgc ccggcacctg cagatgaaag agtgggtcgc acagtgtttg gcggctgacc
     3841 cccggttcac ggacgtggcc accgaaaaac gatggtctgg cacactgacg gcagagtggc
     3901 gaaagcctga tgtgcgtgca atctatcggg gcattccagt ggttttcgag attcagttgt
     3961 ccaccacgta tgtgaatgtg attgccgagc gccgcgagtt ctacttgcag gaaggtggtt
     4021 tgttgatctg gatctttgcc cacttcgacg gcggggcgcg gcggttgact caggacgacg
     4081 tgttttttaa taacaaccgt aatgcctttg tagtgacgca ggcaactcgc gatgcttccg
     4141 tgcaacgggg acggttcatg ctggattgca tctgggccga gccgacattg atgggaaatt
     4201 cagacctgca acgccgggtg gttgggtttg acgacctgac cttggagcgg gagagccagc
     4261 gtgcctacta cttcgatttc gacggagcac gggacgctct gcaagagcag gcccgtgaac
     4321 gagaacgaca acgtcttgcc gaggtacggg agaagttcga gacttggtgg ctcgactacg
     4381 aaacgagcga cacgccggac tatcgtggct gggctgagct acggcgggca ttggcggctg
     4441 aaagggttga acttcctcaa taccccaacc atgtaccccg cacattgttg aatgcgctgt
     4501 actccgcaaa acatgggcgc gtgatcggtt ggcgcttcaa gaccttcatc gaggtcgcac
     4561 accgtgtcga accggcgcac aggcgctacc tgcattattt ccgccgggca ctggctgcct
     4621 acgaccgcgc cgaccagttg cgagccgagg ataaatcggg gaagtgggcg ctcaaggtga
     4681 agcattacaa ggcgcagatg cagatcaacg atcctgccta cacgccggat acctcagata
     4741 tgaaactggt tgttctttta ttcccagaga tctttgtata gcgctgatac ctccggaagc
     4801 cccagagaaa gggcaaggag cgttggccgc gtggcgcaac gccctgccgt agataagtta
     4861 tgctcggttg gtgggcagga cttcaggtgc ttggtcggca tggtgccaaa ccacgacgtt
     4921 gcctgtagtg aagcgctgat ccagatacac cgcctcgatc aacttctcgg gcacgcagat
     4981 gccgagcgca gtgagtttat cggtcagcgt accaatgacg gtccagtcca gggccggttc
     5041 atgaaggctg tcgtagacga tgtcatcatc gccggccggc ccgctctcct ggcagagaaa
     5101 cacctgcagg aaaagttcgc gcaggggccg gtcgtagccg gccactacca agaccggctg
     5161 gccctggtgc atgagccgga tgtggtgccg gctcatgatg cggggtccgg catctcgaag
     5221 atcggcaggc cgtcgatcac ggctgcctcg ctgtcgaagt tcagccagac gacgccggct
     5281 aactgagcga cctcgaaaat cgcggccagg tcggcttcgg tcgggatccg gtggcacggc
     5341 atgccgacgt agaggatgcc gccgcagtcg gtcggatagg cattaaccga caactcgtca
     5401 tcggccagt
//
LOCUS       DAORPM010000172         5362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4299_length_5362_cov_8.504993, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000172 DAORPM010000000
VERSION     DAORPM010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4299_length_5362_cov_8.504993"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1473
                     /gene="tolB"
                     /locus_tag="PLD53_08690"
     CDS             181..1473
                     /gene="tolB"
                     /locus_tag="PLD53_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HRC61032.1"
                     /translation="MLRHLLKLRGLSLIAFSALALFQTVARAELTIEITGAGANRIPV
                     AIAEFAGDPGSSRLLTSVIRGDLERSGLFKLVDANGVAMNESTSPAYSDWKNRGADAL
                     AAGSIVPSGDGRQEARFRLYDINKQATLAGAAFVTSTPMLRAAGHRIADVIYEKLIGE
                     PGYFATRIAYVVKGGPKHYELHIADADGQNAQAALKSKEPIISPSWSPDGSRLAYVSF
                     ENKKPVVYVHSLATGKRIVVANFKGSNSAPTWSPDGRKLAVVLTKEGGSQIFTVNTDG
                     SGAQRLTPTSGINTEPFFSPDGQSIYFTSDRGGSPQIYRISADGGAAQRISFEGSYNV
                     SPRISPDGRSMAFISRRESGFRLAVMDLASKQVQILTESHKDESPTFAPNGRMILIAT
                     EIGGRGVLSAVSIDGRIKQRLSIQAGDVREPAWGPFAR"
     gene            1557..2078
                     /gene="pal"
                     /locus_tag="PLD53_08695"
     CDS             1557..2078
                     /gene="pal"
                     /locus_tag="PLD53_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRC61033.1"
                     /translation="MKQLIIPALLALMIAGCSSTPDGSEQDGAPVESRNSGVATVNAG
                     GIDGRNLPAVLTDPKSILSKRSVYFDYDKYEVKAEYKDLVTAHAKFLVGNRQFKMLIQ
                     GNTDDRGSREYNLALGQKRADAIKQMLTLLGAREDQVESVSLGEEKPKNEGQNETAWA
                     ENRRGDMLYSGEF"
     gene            2149..2898
                     /gene="ybgF"
                     /locus_tag="PLD53_08700"
     CDS             2149..2898
                     /gene="ybgF"
                     /locus_tag="PLD53_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRC61034.1"
                     /translation="MTALTVLLAVFGAQHAQAGVFDDEEARRQIKDLSIKNNERLDTL
                     SKAQFDLVNQIQALREETAKLRGQVETLNYELESTKKRQQDFYIDLDGRLRNLEPQSA
                     APGENNPADGAKPGSDPGKKSAVDPAAVTREYEAALNLFKANKIKEAASAFESFVKAH
                     PDSTLTPNAQYWLGNAHYALRDCNKAIAVHKVVVSKWPQHPKAPDAMINIATCQQELG
                     DNKGEEATLKSVIAKYPDSTAAATAKKRLKK"
     gene            2903..3562
                     /gene="queE"
                     /locus_tag="PLD53_08705"
     CDS             2903..3562
                     /gene="queE"
                     /locus_tag="PLD53_08705"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRC61035.1"
                     /translation="MVGSNNRLSLKVSEIFFSVQGESSRAGLPTVFVRLTGCPLRCVW
                     CDTEYAFTGGQSMTLNRILEEVGTFKTRLVCVTGGEPLAQKNCLLLLTALCDSAYEVS
                     LETSGAIDIAIVDPRVSRVVDLKAPGSGESGKNRWQNLELLTPHDELKFVIKDRTDYE
                     WARNTIVENRLDTVCPVLFSPVQGAIEPRMLAEWILADRLPVRFQLQLHKLLWGNIRG
                     K"
     assembly_gap    3644..3743
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3744..4113
                     /locus_tag="PLD53_08710"
     CDS             <3744..4113
                     /locus_tag="PLD53_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRC61036.1"
                     /translation="EQPEDRRQFSRIAFHTPASLDLPDGRVDVVLLDISLKGALIRLP
                     AETAFGEGAVCVLRIQLDEAESGDLISMDARVAHVEGRNAGLLCRTIDIDSVTHLRRL
                     VELNLGDPILLDREFSALVA"
     gene            4137..4850
                     /gene="queC"
                     /locus_tag="PLD53_08715"
     CDS             4137..4850
                     /gene="queC"
                     /locus_tag="PLD53_08715"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRC61037.1"
                     /translation="MNAAFRSSPQRAVVLLSGGLDSATTLAIARDAGFDCYCLSLDYG
                     QRHRIELEAAATVATALCASEHRVLHLALDEFGGSALTDTRIAIPVDGVQPGIPATYV
                     PARNTIMLSLALAWAEVLGSRDIFVGVNAVDYSGYPDCRPEYIAAFETMANLATKAGV
                     EGRKLTVHAPLIDLSKAEIIRIGSKLGIDYRATVSCYQADQQGMACGVCDSCRLRREG
                     FAAAGVADPTPYMRNPQEA"
     gene            4927..5002
                     /locus_tag="PLD53_08720"
     tRNA            4927..5002
                     /locus_tag="PLD53_08720"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Lys,seq:ttt)
     gene            5049..5124
                     /locus_tag="PLD53_08725"
     tRNA            5049..5124
                     /locus_tag="PLD53_08725"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5082..5084,aa:Lys,seq:ctt)
BASE COUNT         1139 a         1535 c         1482 g         1106 t
ORIGIN      
        1 atgttgaatg tcctatcggg agcgtaaaga cagcctaatt ctacgccgaa acggcatgca
       61 aacagcaata ggcaggcaat acagatccag gcgattgccc cgtaaaatta cgtcccgtta
      121 caaacgcttc acacaccgag gggatataat cgcgcattgt ttatcggaag attttgcccc
      181 atgcttcgac atttgctcaa actgcgcggc ctgagcctta tcgcattcag cgccctggcg
      241 ctttttcaga ccgtagcccg tgctgaactg acgattgaga tcaccggcgc cggggctaac
      301 cggattcccg tggcgattgc cgaatttgct ggagatccgg gtagctcccg tcttctgaca
      361 tcggtcatac gcggcgatct ggaacgcagt gggctgttca agctggtcga tgccaatggt
      421 gtcgcaatga acgaatccac cagcccggcc tatagcgact ggaagaaccg gggtgccgac
      481 gcactggcgg ctggcagcat cgtcccgagc ggtgatggcc gacaggaagc acgcttccgg
      541 ctttacgaca tcaacaagca agccaccctc gccggagcgg ctttcgtcac gtcgacgcca
      601 atgctgcgcg cggcgggcca ccgaattgcc gacgtgatct atgaaaaact tatcggcgag
      661 ccgggctatt tcgcgacgcg cattgcctat gtcgtgaagg gcggcccgaa acactatgaa
      721 ttgcacatcg ccgatgctga cgggcagaat gcacaggcgg cgctgaagtc gaaggaaccg
      781 atcatttcgc catcctggtc gcccgatgga agtcgcctgg cctatgtgtc tttcgaaaac
      841 aagaagccgg tggtttacgt gcattcactg gccaccggca aacgtatcgt cgttgccaat
      901 ttcaagggat cgaattcggc tccgacctgg tcgcccgatg gccgcaagct ggcagtcgtc
      961 ctgacgaagg aaggaggctc ccagatattt acggtcaaca ccgatggctc gggggcacag
     1021 cggctgaccc ccacgtcggg catcaatacc gaaccttttt tcagtccgga cggtcagtcg
     1081 atctatttca cgtcggatcg cgggggtagt ccccagatct acaggatcag cgccgacggc
     1141 ggcgcagcgc agcgcataag ctttgaaggg agctacaacg tttcgcctcg catatcgccg
     1201 gatggcaggt cgatggcttt catcagccgt cgcgagagcg gcttccgcct cgcggtcatg
     1261 gatctggcca gcaaacaggt acaaatcctc actgaatcgc acaaggatga atcaccaacc
     1321 ttcgcgccca atggccgcat gatcctgatc gctaccgaga tcggtggtcg tggtgtattg
     1381 tctgccgtat cgattgacgg ccgaatcaag caacggcttt ccatccaggc cggcgatgtt
     1441 cgcgaacctg cgtggggccc ttttgccaga tagtcaacgg ccccgctctt gttctttgac
     1501 cgtaccctga attttccgaa ttctgaatcg ctctatattc tcgtaggaga aacaccatga
     1561 aacaactcat catccccgcg cttctcgccc tcatgattgc cggctgcagt tcgacgcccg
     1621 atggttccga gcaggatggt gctccggtag agtcccgcaa tagcggtgtc gctacggtta
     1681 acgcgggcgg tattgacggg cgcaatctgc cagccgttct taccgatccg aaaagcatac
     1741 tctccaagcg cagcgtctat ttcgactatg acaagtacga agtcaaggcc gagtacaagg
     1801 atctggtcac tgcgcacgcc aagttccttg tcggcaatcg ccagttcaag atgctgattc
     1861 agggcaacac cgatgaccgt ggcagccgcg agtacaatct ggctctcggg cagaagcgcg
     1921 ccgacgcgat caagcagatg ctgaccctgc ttggcgctcg cgaagaccag gttgaatcgg
     1981 tcagccttgg cgaagagaag ccgaagaacg aaggccagaa tgaaaccgcc tgggccgaaa
     2041 accgtcgtgg cgacatgctt tacagtggcg agttctagga tgctggtgca gcacttgcgt
     2101 ctcgcgtcga gacgttcatc gggtttgaca acgggcctgg caacgggttt gacagcactc
     2161 actgtgctgc tcgccgtttt cggcgcacag cacgcgcagg ccggagtctt cgacgacgag
     2221 gaggcacgcc gacagatcaa ggacctgtca atcaagaaca acgagcgtct cgataccttg
     2281 tccaaagctc aattcgatct cgtcaatcag attcaggcgc tgcgcgagga gaccgccaaa
     2341 ttgcgcggcc aggtcgaaac actgaattac gaactggaat cgaccaagaa gcgtcagcag
     2401 gatttctata tcgaccttga cggtcgcttg cgtaacctgg agccgcagtc agcagcgcca
     2461 ggagagaaca atccggccga cggggcaaaa ccgggctccg atccgggaaa gaagtcggca
     2521 gttgaccccg ccgccgtaac gcgcgagtac gaagctgcgc tcaacctctt caaagccaac
     2581 aagatcaaag aggccgccag cgcctttgag tccttcgtca aggcccaccc agacagcacg
     2641 ctgacgccta atgcgcagta ttggctgggc aacgcgcact acgcgttgcg cgactgcaac
     2701 aaggccatcg cggtgcacaa ggttgtggtc agcaagtggc cacagcatcc gaaggcgcct
     2761 gacgcgatga tcaatatcgc cacctgccag caggaacttg gcgacaacaa gggcgaagaa
     2821 gcgaccctga aatcggtaat tgccaagtat ccggatagca cggcagccgc tactgccaaa
     2881 aaacgcctga agaagtaagc gggtggtcgg cagtaacaat cgcctctcgc tgaaggtgag
     2941 cgagatattc ttttccgtac agggtgaatc ctcgcgtgcc ggcttgccga cggtctttgt
     3001 tcggctcacc ggttgtccgc tgcgctgcgt ctggtgcgat accgagtacg cctttaccgg
     3061 cgggcagagc atgacgctga accggattct cgaagaagtc ggtactttca aaacacgtct
     3121 ggtttgtgtt accggtggcg agccgctcgc tcagaaaaac tgcctgctgc tgctgacggc
     3181 gctctgcgac agtgcttatg aggtctcgct ggagacctcc ggtgcgatcg acatcgccat
     3241 cgttgacccg cgggtttccc gggttgttga cctcaaggct cccggctcgg gagagtccgg
     3301 caagaatcgc tggcagaacc tcgaactgct cacgccgcat gacgagttga agttcgtaat
     3361 caaggaccgc accgactacg agtgggcacg aaatacgatc gtcgagaacc ggcttgacac
     3421 agtctgcccc gtgctcttct cgccggtgca gggtgcgatt gaaccgcgaa tgctggctga
     3481 atggatactc gctgatcgat tgccggtgcg ttttcaactc caactgcaca agctgctttg
     3541 gggtaacatt cggggcaagt aaccgcgcgg acacattgcc atgaacgctg aacagcctga
     3601 agatcgacgc caattctcgc gtatcgcttt ccacactcca gccnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnntgaacag cctgaagatc gacgccaatt ctcgcgtatc
     3781 gctttccaca ctccagccag cctggatctc cctgatggac gtgtcgatgt cgtgctcctc
     3841 gacatttcgc tgaaaggcgc gctgattcgc ctgccggccg aaacggcatt cggtgagggg
     3901 gcggtttgtg tgctgcgcat tcaactcgac gaagccgaat caggcgatct gatcagcatg
     3961 gacgcccgcg tcgctcatgt tgaaggacgc aacgccggcc ttctttgccg gacgatcgat
     4021 atcgacagcg tgacgcactt gcgccgtctg gtagagctca atctcggtga tccgatactg
     4081 cttgatcgcg agttctcggc cctggtcgcc tgatattttt cagtcagggc tacgcaatga
     4141 atgccgcttt tcgatccagt ccgcaacgtg ctgtcgtact gctctccggc ggcctcgact
     4201 cggccaccac gctggccatc gcccgtgacg ccggtttcga ttgctactgc ctttccctgg
     4261 actatggtca acgccatcgc attgaacttg aagcggccgc cacggtagcc acggcgttgt
     4321 gcgccagcga gcatcgcgta ctgcatctgg ctcttgacga gttcggcggt tcggcgctca
     4381 cagacacccg catcgcgata cccgttgacg gcgttcagcc cggcattccg gccacctacg
     4441 tgccggcacg caataccatc atgctctctc tggcgctggc ctgggccgag gtattgggca
     4501 gccgtgatat tttcgtcggc gtcaatgctg ttgactactc cggctacccg gattgtcggc
     4561 cggaatacat cgccgctttc gagaccatgg ccaatctggc aaccaaggcc ggtgtggaag
     4621 ggcgcaagtt gactgttcat gctcctttga tcgatctttc caaggccgag atcattcgca
     4681 tcggcagcaa gctgggtatt gactatcgcg caaccgtttc ctgctaccag gcagaccaac
     4741 agggcatggc ctgcggagtt tgcgattctt gccgattgcg acgggagggt tttgctgcgg
     4801 ctggcgttgc cgacccaacc ccttacatga ggaatccaca agaagcgtag caacggcatg
     4861 cgtgccaagg gcctgcattt gacaggtttc cgggtggtat ctataatccg cccttcctgt
     4921 tttttcgggt cggtagctca gttggtagag cagcggactt ttaatccgtt ggtcgagagt
     4981 tcgaatctct cccgacctac caagacacta ccgatcttgg tggaagcgct agttggtggt
     5041 tactccgtgg ggcgttagct cagttggtag agcagcggac tcttaatccg taggtcgagt
     5101 gttcgaatca ctcacgccct accacgaaat gtaatttaaa aacaatacct tagaagtgaa
     5161 ttggcccaac caaaaaaggt tgggctttgt tctttgtggg ccatggccca caactggccc
     5221 tttcgtggcc cacgagaacc gaccactatg ctgaatttga actaaagacc aatcgggcca
     5281 cagtcggcaa gaacaaggca ttttcaagag tggctattcc gataacctat tccatcttca
     5341 agaagtctgc ccaccatcaa tg
//
LOCUS       DAORPM010000173         5338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4324_length_5338_cov_9.388037, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000173 DAORPM010000000
VERSION     DAORPM010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4324_length_5338_cov_9.388037"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PLD53_08730"
     CDS             <1..1252
                     /locus_tag="PLD53_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61038.1"
                     /translation="PKPKPVVISRKKTETKTDSTDRLVLMPADEPRVEPGIESTPPEK
                     LVSPGSSGEIGSRLVDLEQQIVTMRSQLSALRAEYPAPPPAIQTVFIEMESRLLAVEL
                     SVARVKLSTLATDASVNATAAETQVNSASNEKANIAPTPPPTPAAAPLQAAGKPLTDV
                     AEPSSFGTGLLMLGMFGLGLGAFLFRRARRASTVPTPASAVTSPANEVAHSVREIPAQ
                     QTIPPATREVDEMAEAQKVLDAMLPTRATEDTRKEDLDFSPIELANIMLSFGRAQGAV
                     EVLQDFVRENPNESLQPGLRLLEIYKQTDMRREFEQMAGQLAHRFNVERVHWNQDFLP
                     ATEAIPSGVDARRTPGNLSVMPMHIKARIAANWGTSDCLAYLQSLLSDTRGGARKGFS
                     VLVTQEILGVIKALEAQLKSRKTA"
     gene            1404..>2299
                     /gene="hemA"
                     /locus_tag="PLD53_08735"
     CDS             1404..>2299
                     /gene="hemA"
                     /locus_tag="PLD53_08735"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRC61039.1"
                     /translation="MAIYSIGINHRTAPLALRERVAFHNEELRRALTDLSSSGRVHEA
                     AILSTCNRTEIYCQADSPLATTQWLAQYRHLPHHELEPYFYHHPDREAVRHAFRVASG
                     LDSMVLGEPQILGQMKDAVRIAREAGTLGVTLNKLFQHTFAVAKDVRSTTAIGSNVVS
                     MAAAAVRLAERIFERIANQRVLFIGAGEMIELCAAHFAAQKPKQIVIANRTIDRGRKL
                     ADRFGATAIRLEDVGERLAEFDIVVSCTASQLPIIGLGLVERAIRARRHRPIFMVDLA
                     VPRDVEVDVGELDDVFLYTVDDL"
     assembly_gap    2300..2399
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2406..3488
                     /gene="prfA"
                     /locus_tag="PLD53_08740"
     CDS             2406..3488
                     /gene="prfA"
                     /locus_tag="PLD53_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRC61040.1"
                     /translation="MKQSIRDKLEGLAGRLDELDRTLSSSEATRDMDQYRKLTREHAE
                     LGPVVALYQTWRQTEADLQSALDMMTDPEMKELAEAEMKSASERLPEIERELQNLLLP
                     KDSDDERNVFLEIRAGTGGDESALFAGNLFRMYSRFAERQRWQVEVVSSSASDVGGYK
                     EVIARIVGNGVYSRLKFESGTHRVQRVPETEAQGRIHTSACTVAIMPEADELEDVQIN
                     PAEIRIDTFRASGAGGQHINKTDSAIRIVHLPTGIVVECQDGRSQHKNKAQAMSVLVA
                     RIRDAQQREQNAMIASTRKSLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDSIMD
                     GDLDELLGALANEHQAEQLTALGGAE"
     gene            3493..4416
                     /gene="prmC"
                     /locus_tag="PLD53_08745"
     CDS             3493..4416
                     /gene="prmC"
                     /locus_tag="PLD53_08745"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRC61041.1"
                     /translation="MSCAELSAGIGHVWRLASQRVDRLDARFLLQHVSGCTLAELIAH
                     PERELSAEQADRFDGLVSRREAGEPLAYLLGTAFFCGLEFAVSPAVLIPRPETEVLVE
                     QSRRRVQELLQMRCQEQAQSRALACIAPRIVDLGTGSGIVAIMLARLCPTAELTAVDV
                     SVAALYVARANAVRHGVQIRFLEGDWYASLGKERFDLIVSNPPYVADGDLHLQQDGLP
                     FEPRLALSDGVVGGDGLGCIRTLIDGAKNHLFPGGWMFIEHGYDQAVQTRELLHAAGF
                     TDVDSWCDGAAIERVSGGRQPPVNPRNFSKL"
     gene            4439..4762
                     /gene="grxD"
                     /locus_tag="PLD53_08750"
     CDS             4439..4762
                     /gene="grxD"
                     /locus_tag="PLD53_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRC61042.1"
                     /translation="MDVQQLIKEQVTGNPVALYMKGTPKAPMCGFSAMAVQILQACNL
                     PLFFSVNVLEDQAIRDGVKEFANWPTIPQLYIRGEFVGGSDIMREMYQSGELKAMLEG
                     IAAPD"
     gene            4868..>5338
                     /locus_tag="PLD53_08755"
     CDS             4868..>5338
                     /locus_tag="PLD53_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61043.1"
                     /translation="MMLSRSVVMGAVVLLAACAGYSGSNLKPGLSTLPEVVASMGEPA
                     LRWKDPDGGEQLAYPRGPAGTQTFMVFMGADGRLDRIDGVLDMAHFARIESGKSDKAA
                     VLRLLGPSQPQWTEYFKARDELVWEWRFCDSWGKVALFDVLFDATTGIVRTTYQR"
BASE COUNT         1058 a         1442 c         1641 g         1097 t
ORIGIN      
        1 gccgaagccc aagcccgttg tcatctcgcg caagaagacc gaaacaaaaa cagacagtac
       61 tgatcgattg gtgctgatgc cggctgacga accgcgtgtc gaaccgggta tcgagtccac
      121 tccgccggag aagttggttt ctccgggcag ttccggcgag attggaagcc ggctggtcga
      181 tcttgaacag caaatcgtca caatgcgctc gcagctgagt gcgctacgtg ctgaatatcc
      241 ggcacctcca ccggccatcc agacagtgtt catcgaaatg gaatcccggc tgcttgccgt
      301 ggaactgagc gtcgcgcgcg tcaagttatc gacgttggcg accgatgcgt ctgtgaacgc
      361 aactgcagct gaaacgcaag tcaactcggc aagcaacgaa aaggcgaata tcgctccgac
      421 gcctcccccg accccagccg cggcgccttt gcaggcggcc ggcaagccgc tgactgatgt
      481 ggcggagcca agttcgttcg gtaccggcct gctgatgctg ggaatgttcg ggcttggcct
      541 gggcgctttt ttattccgtc gggccaggcg cgccagcacc gtgccgactc cggcaagcgc
      601 tgtaacaagc ccggcaaacg aagttgcaca ttcggttcgc gaaataccgg ctcaacagac
      661 aattcctcct gcaacaaggg aagtcgacga aatggcagaa gcgcagaagg tgcttgacgc
      721 gatgctaccg acccgtgcca ccgaagacac caggaaggaa gatctggact tcagcccgat
      781 tgaactggcc aatatcatgc tcagcttcgg ccgcgctcag ggcgcggtgg aggtccttca
      841 ggattttgtc agggaaaacc cgaatgagtc gttgcagccg ggcctccgtc ttcttgaaat
      901 ctacaagcag accgacatgc gccgggagtt cgaacagatg gccggtcagc tcgctcatcg
      961 attcaacgtc gagcgcgtgc actggaatca ggatttcttg cctgctaccg aagcgatccc
     1021 ttcgggtgtc gatgcacgcc gcacgccggg caacctgagc gtcatgccca tgcatatcaa
     1081 ggcgcgcatt gcggcaaact ggggtactag cgattgtctc gcttaccttc agtcgttgct
     1141 aagcgacaca cgcggcggag cgcgcaaggg tttctcggtc ctggtcacgc aggagatact
     1201 gggcgtaatc aaggcgctgg aagcgcagtt gaaatccagg aaaaccgcat agacgatcct
     1261 ccagactacg cgtcacctcg taccatcatt gcgcagtcaa gcttcgccac ttctcgccga
     1321 gcagaggcgt ggaggggcgg gtgctataat cggccgcttc ctctgctgat cgcgtgccct
     1381 aacttccgcg tactgaaccc cgcatggcga tttattctat cggcatcaat catcgcacgg
     1441 cgccgctcgc gctccgcgag cgggtggcgt tccataacga agagctaagg cgggcgctga
     1501 ccgatttgtc gagtagtgga cgggtgcatg aagcggcgat tctgtcgacc tgtaatcgca
     1561 ctgagatcta ctgtcaggca gattctccac tggcgaccac tcaatggctt gcgcaatacc
     1621 gccatttgcc gcaccacgaa ctggagccgt atttctatca ccatcccgat cgtgaagcgg
     1681 tgcgccacgc ttttcgcgtt gccagcgggc tggattcgat ggtactcggt gaaccgcaga
     1741 ttcttggcca gatgaaggat gctgtgcgta ttgccaggga ggcgggtacg cttggtgtga
     1801 ctttgaacaa gctgttccag catacctttg cggtggccaa ggatgtgcga tccacgaccg
     1861 ccatcggcag caatgtcgtg tcgatggctg ccgctgcagt tcgcttggcg gagcgtatat
     1921 ttgagcggat cgccaatcag cgcgtacttt ttatcggtgc gggtgaaatg atcgagttgt
     1981 gcgcggccca cttcgcggcg cagaaaccga aacagatcgt tattgccaac cggacgattg
     2041 atcgggggcg caagctcgcc gatcgattcg gggcaacagc aattcgcctg gaggatgtcg
     2101 gtgagcgtct ggccgaattc gatatcgtcg tttcctgcac tgccagccag ttgccgatca
     2161 tcggtttggg gcttgtcgaa cgggccattc gcgctcgccg ccaccgcccg atattcatgg
     2221 tcgatcttgc cgtgccgcgc gatgtcgagg tcgatgtggg tgagctggac gacgtctttc
     2281 tctataccgt cgatgatctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2401 ttttcatgaa acagagcatt cgcgacaaac ttgaaggtct ggccggccgg ctggacgaac
     2461 tggatcgcac gctgtcgagc agcgaggcga cgcgtgacat ggatcagtac cgcaagttga
     2521 cccgcgagca cgccgagctt ggtccggtcg tcgcgctcta ccagacttgg cgtcagaccg
     2581 aggctgatct gcagtcggcg ctcgatatga tgaccgaccc ggagatgaag gaattggccg
     2641 aagcggagat gaaatcagca agcgagcgct tgcccgagat cgaacgcgaa ttgcaaaatt
     2701 tattgttacc gaaggattcc gacgacgagc gtaacgtctt tctggagatc cgtgccggaa
     2761 cgggtggcga cgagtcggcg ttgtttgccg ggaacctgtt tcgcatgtat tcgcgctttg
     2821 ccgagcgtca gcgctggcag gtcgaagtcg tttcctccag cgcttcggat gtgggcggct
     2881 acaaggaggt gattgcacgc attgtcggca atggcgtgta ttcaaggctg aagttcgagt
     2941 ctggcacgca tcgcgtgcag cgtgttccag agaccgaagc gcaaggccgc atccacacct
     3001 cggcgtgtac tgtagccata atgccggaag ccgacgaact ggaagacgtc cagatcaatc
     3061 cggcggagat tcgcatcgat acttttcgcg catcgggcgc tggtgggcag cacatcaaca
     3121 agaccgactc ggcgatccgt atcgttcatt tgccgaccgg catcgttgtc gaatgccagg
     3181 acggtcgttc gcagcataag aacaaggcgc aggccatgtc ggtgctggtg gcgcggatca
     3241 gggatgcaca gcaacgcgag cagaacgcca tgattgcatc gacacgcaag agtctggtcg
     3301 gtagcggcga tcgttccgag cgcattcgca cgtataactt cccgcaaggg cgagtgaccg
     3361 atcaccgcat caatttgacc ttgtacaaga tcgattcgat catggacggc gacctcgacg
     3421 aactgctcgg cgcgctcgcc aacgagcatc aggctgaaca gcttaccgcc ctcggagggg
     3481 cggagtaagc gcatgagctg tgctgagctg agtgctggaa ttggccatgt ctggcgcttg
     3541 gccagccaac gcgtcgaccg gctggatgcc cggtttctgc tgcagcatgt cagtggctgc
     3601 acgcttgccg agctgattgc gcatccggaa cgcgaattat cggctgaaca ggccgaccgg
     3661 ttcgatgggc tggtttcgcg tcgcgaggcg ggcgaaccgc tggcttattt gctgggaacg
     3721 gcctttttct gcgggctgga attcgccgtg tcgccagcgg tgctgattcc acggcctgag
     3781 acggaggttc tggtcgaaca atcaagacga agagtgcagg agttgttgca aatgcggtgt
     3841 caagagcagg cgcaatcgcg ggcgctagcg tgcatcgcac cgcgcatcgt tgatctgggc
     3901 accggctcgg gcatcgttgc catcatgctc gcccggcttt gtcctactgc agaactgacg
     3961 gcggttgatg tgtcggtggc ggcgctctat gtggcacgtg cgaacgcggt tcggcatggc
     4021 gtacagatcc gttttctcga aggggactgg tacgcatcgc tcggcaaaga gcggtttgac
     4081 cttatcgttt ccaatccgcc gtatgtcgcc gatggcgatc tgcacttgca gcaagacggt
     4141 ttgccctttg agcctcggct cgcgctgtcc gatggtgtgg tcggtggcga cgggctgggg
     4201 tgcattcgca cgcttatcga tggggcaaag aaccatcttt ttccgggcgg ctggatgttt
     4261 attgaacacg gctacgatca agcggtccaa acccgcgagt tgctgcatgc tgcaggtttc
     4321 accgatgtgg attcctggtg cgatggtgcg gcgattgaaa gggtaagcgg cggtcgccaa
     4381 ccgcccgtta acccgcgaaa cttctcgaag ctttaatcga aacctatgga ggtattgaat
     4441 ggacgtgcag caactgatca aggagcaagt cacgggcaat cctgtggcgc tctatatgaa
     4501 gggaacaccg aaggctccga tgtgcggttt ttccgcaatg gccgtacaaa ttctgcaagc
     4561 ctgcaatctg ccgctgttct tcagcgtcaa tgtgctagaa gaccaggcga tacgtgacgg
     4621 cgtcaaggaa ttcgccaact ggccgaccat ccctcagctt tacattcgtg gcgagtttgt
     4681 cggcggtagc gatatcatgc gcgagatgta ccagagcggc gagctgaaag cgatgctcga
     4741 aggtatcgcc gccccggact gattcgacca tatcagtccg gcgtgcgctg gcgtgatgtt
     4801 gcgtcatccg acgggggcga cgatttttcg attggatgaa gacggacaat acgaagcagg
     4861 agccaatatg atgctgtcac gttcggtggt tatgggtgcc gtagtgttgc tcgcggcctg
     4921 tgccggatac agcggcagca acctgaagcc ggggttgtct acccttcctg aggtcgttgc
     4981 ttcgatgggg gagcctgcac tgcgctggaa agatcccgat gggggtgagc aactggccta
     5041 cccgcgcggg ccagccggca cgcagacctt catggtgttc atgggtgcgg atggccgcct
     5101 ggacaggatt gacggtgtgt tggacatggc gcatttcgca cgtatcgagt caggaaaaag
     5161 tgacaaggcg gcagtgctgc ggcttctggg gccttcgcaa ccgcagtgga cggaatactt
     5221 caaggcgcgc gacgaactgg tgtgggaatg gcgcttctgc gatagttggg gcaaggtggc
     5281 gctctttgat gtgctgttcg acgcgacgac cggtatcgtt cgcacgacat accagcgt
//
LOCUS       DAORPM010000174         5283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4394_length_5283_cov_9.036917, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000174 DAORPM010000000
VERSION     DAORPM010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4394_length_5283_cov_9.036917"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..994
                     /locus_tag="PLD53_08760"
     CDS             11..994
                     /locus_tag="PLD53_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRC61044.1"
                     /translation="MPTKTRKSPFRTPADAAPRLRGAPSRDPGNTRPETEESASAPKP
                     RRKPLVTPSDPSKRGRHKEQPGKPERLHKLLAQSGIGSRREMEELIAAGRININGETA
                     RIGQSASPGDRIKVNGKLVHLKFSNRLPRVIIYHKPEGEIVSRDDPEHRPNVFTALPR
                     MSGGRWVAVGRLDFNTSGLLLFTTSGDLANRLMHPRYNLVREYAVRILGALSEEARQR
                     LLDGIELGDGPARFDTLQEAGGEGANHWYRVSLFEGRNREVRRMFEAVGVVVSRLMRV
                     RYGPFILPPNLKRAQVLELGEGEVKKLLSDFGMDDPAPGRPIHKPRERYAG"
     gene            complement(1015..2559)
                     /locus_tag="PLD53_08765"
     CDS             complement(1015..2559)
                     /locus_tag="PLD53_08765"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRC61045.1"
                     /translation="MDGIFISYRRDDSAGYAGRLYDRLVAHFGPNQVFMDVEEIEPGL
                     DFVVAIEEAVSSCRVLIAVIGDEWTTGTDAAGRRRLDDPNDFIRLETSSALQRGIRVV
                     PVLVGGAVMPLSTELPEDLRSLTRRQAIEINHKQWEASTGELIRTLENILKTPRADPA
                     AASRPPNDGDVVTVDAAPRGKASNGYANQGKSRPWILPAAIAAAAVVAIGVWMATGDK
                     KSGTKTEKQPFSSAAKSPVPTASGTQATSPSTAPPTTPTVVETKPVTAPKVDPTPTAR
                     PAPTPTPVTPAAVPPPVVRPASAPKPVIATRPDKESAKAAAPADLAPSPLPVKGERWT
                     YRSSGKWATSPKRRFEIVAQSEAGGVVTDELRASEPADRTEVHRARSNKPDFIAWNVI
                     GPEFSPYLGAYVDLARQNSFSDLPTPDLEPQWRDWYSSAKVLGKESVQVPAGTFDAFK
                     VEVWSSRHQTGSRAEASIEPLRVNYLIWYAPQAKRYVRMERRVTTADNSESEKDVFEL
                     VSHRLP"
     gene            2814..3047
                     /locus_tag="PLD53_08770"
     CDS             2814..3047
                     /locus_tag="PLD53_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRC61046.1"
                     /translation="MPIAFPVRGDYIQLDQLLKATGLCPSGGHAHAEIGAGQVLVDGS
                     IELRKRAKLRAGQQVRYGGEIVDLMPESIPEGA"
     gene            3114..4193
                     /locus_tag="PLD53_08775"
     CDS             3114..4193
                     /locus_tag="PLD53_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRC61047.1"
                     /translation="MLWFVLGLVALVLGAELLVRGASKLALSIGISPLVVGLTVVAFG
                     TSAPELAVSVQSAWSGQVDIALGNVVGSNMFNILVVLGFSALIVPLVVHQQIIRQEVP
                     VMIGASLLLWAMAADGGISRLDGLLLASLVVAYTILVIHQSRRETAVIRTEYKEAFEA
                     APTGWDAHWGIQVLMLLSGLALLVLGAHWLVDAAVAFAHYLGVSELIIGLTIVAVGTS
                     LPEGATSIMAALRGERDIAVGNAVGSNIFNLLSVVGFSASVAPSDLVVAPAMISFDLP
                     IMVAVAVACLPVFFNGACISRGEGFLFLALYVAYTLYLYLHASSHAALDGYSMVMGLF
                     VLPLVAVALLVLAWRHWHRRRVGKD"
     gene            4424..>5283
                     /locus_tag="PLD53_08780"
     CDS             4424..>5283
                     /locus_tag="PLD53_08780"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HRC61048.1"
                     /translation="MLESYRAHVAERAALGIPPLPLTAKQTTELVELLKNPPKGEEKA
                     LVELIAHRVPAGVDDAAKVKAEYLAKVAKGEESCTLISKAKAVELLGTMLGGYNVKPM
                     IDVLGDAEVGAVAAEGLKKTLLVFDFFHDVKELADKGNANAKAVMQSWADAEWFTSRP
                     EVPASQKLTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGIEADEPGTRG
                     PMKQLETLAAKGNLIAYVGDVVGTGSSRKSATNSVLWWTGEDIPFVPNKRFGGVCLGS
                     KIAPIFFNTM"
BASE COUNT          972 a         1557 c         1616 g         1138 t
ORIGIN      
        1 gaactggtcg atgcccacga aaacccgtaa gtccccattt cgaacacctg ccgacgcagc
       61 gccgcgtttg cgcggcgcgc ccagccgtga tcccggaaac acccgaccgg aaacggagga
      121 aagcgcatcc gctccaaagc ctcgccgcaa acctcttgtt acaccgtcgg atccgtcgaa
      181 gcgcggacgc cacaaggagc agccgggcaa acccgagcgt ctgcacaaac tactcgcgca
      241 gagcggaatc ggttcgcggc gcgagatgga ggaactgatt gccgccggac gcatcaatat
      301 caacggcgaa accgcgcgca tcggccagtc ggccagtccg ggcgaccgga tcaaggtcaa
      361 cggcaagctg gttcatctga agttctcgaa ccgcctgcca cgcgtgatca tctatcacaa
      421 accggaaggc gagatcgttt cacgcgatga tcccgaacac cggccgaacg tttttaccgc
      481 actgcccagg atgtccggcg ggcgatgggt tgccgtcggt cgtctcgatt tcaataccag
      541 cggcctgctg ctatttacta cctcgggcga tctggccaat cgcctgatgc acccgcgcta
      601 caacctcgtc cgcgagtacg ccgtgcgcat cctcggtgcg ttgtccgaag aggcgcgcca
      661 gcgattgctc gacggcatcg aactgggcga cggcccggcc cggtttgata ccctccagga
      721 agcgggtggc gaaggcgcca atcactggta tcgcgtttcg cttttcgaag gccggaatcg
      781 cgaagttcgg cgcatgtttg aagcggttgg cgtcgtcgtc agtcgactga tgcgcgtgcg
      841 ctacggcccc ttcatcctgc cgccgaacct caagcgcgct caggtactcg aattgggcga
      901 gggcgaagtc aagaagctgc tgagcgactt cggtatggac gatcccgcac cgggccgacc
      961 catacacaag ccgcgcgagc gctacgcggg ataaccggtt gtccgcagac ggtgctacgg
     1021 taaccggtga gaaaccagct caaacacgtc cttttcgctt tcgctgttgt ccgcagtcgt
     1081 aacgcggcgt tccatccgca catagcgttt tgcctgtggt gcgtaccaaa tgaggtagtt
     1141 cacgcgcaac ggctcgattg atgcctcggc acgcgagcca gtttgatggc gactgctcca
     1201 gacctccacc ttgaaggcgt cgaaagtacc ggctggcact tgcacggact ctttgcccag
     1261 caccttcgcg ctggaatacc agtccctcca ttgcggctca agatcgggtg tgggtaagtc
     1321 gctgaacgag ttttgccgcg cgagatcaac ataggcgccc agataggggc tgaattcagg
     1381 tccaatgacg ttccaggcga tgaagtcagg tttgttgctg cgtgctcgat gcacttcggt
     1441 gcgatcggcc ggctcgctcg ctcgcaattc gtcggtaaca acaccgccag cttccgactg
     1501 tgcgacgatc tcaaaacgcc ttttcgggct ggtggcccac ttgcccgaag agcggtaggt
     1561 ccaacgctct ccctttaccg gcagtggtga aggagcaagg tccgccgggg cggcggcctt
     1621 tgcagattcc ttgtccgggc gggtcgcgat gactggtttg ggcgcacttg ccgggcgaac
     1681 gacaggtggg ggcaccgccg ctggcgtgac gggtgtcggg gtcggcgcgg gtcgagcggt
     1741 gggggttgga tcgacttttg gcgccgtcac gggtttggtc tcaacgactg tgggcgttgt
     1801 gggtggcgct gtggacgggc tcgtggcctg tgttccggat gcagttggga ccggcgattt
     1861 ggcagcgctg ctgaagggtt gcttttcagt cttggtacct gatttcttgt cgcccgtagc
     1921 catccacacg ccgatggcga cgaccgcggc agcggcaatg gcagcgggca gaatccatgg
     1981 cctggatttg ccctggttcg catatccatt gcttgccttg ccgcgcggtg ctgcatcaac
     2041 agtcacaacg tctccgtcat tcggtggccg cgatgccgct gccggatcgg cacgcggcgt
     2101 cttgaggatg ttttccagag tgcggatcag ctcgcccgtg gaggcttccc attgtttgtg
     2161 attgatttca atggcttggc gccgggtgag cgatctcaga tcttcaggca gttccgtgct
     2221 tagcggcatg acagcgccac cgaccagcac cggaaccacg cgtattccgc gttgcagtgc
     2281 ggagctggtt tccagccgga tgaagtcgtt gggatcgtcc agccggcgtc ggccagcggc
     2341 gtcggtaccg gtggtccact cgtcgccgat aacggcgatc aatacacggc atgagctcac
     2401 ggcctcttcg atggccacca cgaaatcgag accgggctca atctcttcga catccatgaa
     2461 aacctggttg gggccaaaat gggcaaccag gcggtcgtac aaacgaccgg cgtatcctgc
     2521 cgaatcgtcg cgccgatagc tgatgaaaat accgtccata ccgaactacc tcctcgcatc
     2581 acaggactgt cggcaggccg ctacagtctg cgtgatggtt ttcagtcggg gccatgatga
     2641 agcatcagcc gcccaggcgc aaccaccgcc aaagggttgg ctggtgtcaa aatcgtcttg
     2701 ggatctacac gatctggggg catcgcaact cacccgaaac cgtgcgcgcc aaatctgcga
     2761 gaattgggtc tacggtgcca agtctgcgcc attccttctc gtcaaggata ctcatgccca
     2821 tcgcttttcc ggtccgtggt gactacattc agcttgacca gttgctcaag gcaaccggtc
     2881 tgtgcccttc tggcggacat gcgcacgctg aaatcggggc agggcaggtt cttgtcgatg
     2941 ggagcatcga gttgcgtaag cgcgccaagc tgcgcgcggg tcagcaggtc cgttatggtg
     3001 gcgagatcgt tgatctgatg ccggaaagca ttcccgaagg cgcataggag tccgggtttg
     3061 cgacatgccg gagttgtccg caatgcgcaa tcggtttttt tggagttgtt gagatgttgt
     3121 ggtttgtgct cgggctcgtt gccctggttc taggtgcgga gctgctggtg cgtggtgcgt
     3181 ccaaacttgc gctatcgatc ggcatctcgc cgctggtggt cggccttacc gtggttgcat
     3241 tcggcaccag cgcacctgaa ctggcggtgt cggtgcaatc ggcctggtcc ggccaggtcg
     3301 atattgcgct cggtaatgtg gtgggcagca atatgttcaa catattggtc gtcctgggct
     3361 tctcggcctt gatcgtccca ctggtcgtgc atcagcagat catccgccag gaggtgccgg
     3421 tgatgatcgg cgcttcactg ctgctctggg cgatggccgc cgacggcggc atcagtcgcc
     3481 tggatggcct gctgttggca tctctggtcg tggcttacac catcctcgtc atacaccaga
     3541 gccgacgcga gacggccgtc atcaggaccg agtacaagga ggcctttgaa gcagcgccca
     3601 cggggtggga cgcccattgg ggcatacagg ttctcatgct gctttcgggg ttggcactgc
     3661 tggtgctggg cgcgcactgg ctggtcgatg ctgcagtagc ttttgcccat tacctggggg
     3721 taagcgagct gatcatcggc ctgactatcg ttgccgtcgg cacctcgttg ccggaaggag
     3781 cgacttcgat catggcggcc ttgagaggtg agcgtgatat cgccgttggc aatgcggtgg
     3841 ggagcaacat tttcaatctg ttgtcggtgg tgggcttctc cgccagtgtg gcgccctccg
     3901 atctcgtcgt ggcgccggcg atgatctcat tcgatctgcc gatcatggtg gcggtcgccg
     3961 tggcctgtct gcccgtattt ttcaacggtg cctgcatttc tcgcggtgaa ggattcttgt
     4021 tcctggccct ctatgtggct tacacgctgt atctgtactt gcatgccagc agccatgcag
     4081 cactcgatgg ttacagtatg gtgatgggcc tcttcgtcct gcctcttgtg gccgtcgcct
     4141 tgctggtgct ggcctggcgg cactggcacc gccggcgggt cggcaaggac tgatcaatgc
     4201 aaaacccaaa agattgactt cacagcagaa cgcttatctt atatcatata taagagtcgc
     4261 catttggtgg tctgccattc ccccaaatag ttcgcaattt cggagacgac agcaaccaga
     4321 gcgctgttgc cgtttctgcc ttgaagtaca atgatcgtct aatccttgcc tttcgatttt
     4381 catatttctg cccttagacc aacctttgtg aaaggacaat cccatgctgg aatcctatcg
     4441 tgcccacgtc gccgagcgcg ctgctcttgg tatcccgccc ctcccgctga ccgccaagca
     4501 gaccaccgag cttgttgaat tattgaagaa ccctccgaag ggtgaagaaa aggcactggt
     4561 cgagctgatt gcccaccgtg ttcctgccgg tgtcgatgat gctgccaagg tcaaggccga
     4621 atatctcgcc aaggtcgcca agggcgaaga aagttgtacg ctgatttcga aggccaaggc
     4681 ggtcgaactg ctcggtacca tgctcggcgg ctacaacgtc aagccgatga tcgatgtgct
     4741 gggcgacgcc gaagtcggcg ccgttgcggc cgaaggactc aagaagaccc tgctggtatt
     4801 cgatttcttt cacgacgtga aggagctcgc cgacaagggt aatgccaacg ccaaggctgt
     4861 aatgcaatcc tgggccgacg ccgagtggtt tacctcgcgt cccgaagtgc cggcatcgca
     4921 gaaactgacc gtcttcaagg tcactggcga gaccaatacc gatgatctat cgccagcgcc
     4981 cgatgcctgg tcgcgacccg atattccact gcacgcactg gccatgctca agaacccgcg
     5041 tccgggtatc gaagcggatg aaccgggcac ccgtggcccg atgaagcagc ttgagacact
     5101 cgccgccaag ggcaacctga tcgcctatgt cggcgacgtg gtcggcaccg gctcctcgcg
     5161 caagtcagcg accaactcgg tcctctggtg gacgggtgag gacatcccct ttgtgccgaa
     5221 caagcgtttc ggtggcgtct gtctcggttc caagatcgcg ccgatcttct tcaacaccat
     5281 gga
//
LOCUS       DAORPM010000175         5239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4458_length_5239_cov_9.133102, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000175 DAORPM010000000
VERSION     DAORPM010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4458_length_5239_cov_9.133102"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLD53_08785"
     CDS             <1..223
                     /locus_tag="PLD53_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61049.1"
                     /translation="ISSLKLGQQIRSRNLRVAEQTAPTGSVFSSQTQRDGNAARPDLP
                     TETVDKPVELLCAHQDKPCAGEGRKKLPN"
     gene            complement(253..1929)
                     /locus_tag="PLD53_08790"
     CDS             complement(253..1929)
                     /locus_tag="PLD53_08790"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HRC61050.1"
                     /translation="MDKVWLQSYQDGVPAEVDLNEFQSLGEMFEKSFSQYRERVAYIN
                     MGVELSYGELDKLSRDFAAYLQAVLGLPLGSRIALMMPNVLQYPICMLGALRAGYTVV
                     NCNPLYTPRELEHQLKDSEAEAIVILENFAHTLERVIDKTPIRHIVVARLGDMFGFPK
                     GRVVNFVVKYFKRMIPPWKLPGAVHFGQALTKGAVADFTPVQVTQEALAFLQYTGGTT
                     GLSKGAMLTHRNVLANLAQAHAWIRPVVTDGGEFIVTALPLYHIFALTANCFTFIKIG
                     ASNLLITNPRDIPAFTAELGKYPFTVITGVNTLFNALLGNEDFNALDFSKLKLAMGGG
                     MAVHQAVAEKWKRITGLPLIEAYGLTETSPAATINPLNISDYTGSIGLPISSTEVAIR
                     NDIGEDVPLGQRGELCIRGPQVMMGYYNRPDETACVLMQDGFLRTGDIAVMDEKGYVR
                     IVDRKKDMILVSGFNVYPSEVENVVAMHPGVMEVAAIGVPDERSGEAVKIFVIRKDQT
                     LTAEQLVAHCREYLTAYKVPSHVEFRAELPKSNVGKILRRELRDEASNAA"
     gene            complement(2036..>5239)
                     /locus_tag="PLD53_08795"
     CDS             complement(2036..>5239)
                     /locus_tag="PLD53_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRC61051.1"
                     /translation="LLASRIVRLLLAGVSPGEILAITFTRKAAREIEERVVDWLHLLA
                     TGSDEQVLAFLSERGLAVDEGEGTTIRAARGLYERVLVAQPGLAVNTFHGWFLQLVDA
                     APLSANLTGATLIESDSRLFDELWQSFASSLQKLPESEVTHSFVRLLDEAGLDQTRRM
                     IRRALERRSEWLAFAGEKSDTAGKVVAALRELLAAGAPGQAMDAFFAGDWALEFQNYL
                     GLLETSELSTDQKYVRILRAGLLEPVRERSLDLLREVLLTTTNTVRKREVNTASEKRF
                     TPEGAARLVALHGTLSQRVLDCIEQQTAERILAFNQNACTVFAAFLKHVDEFKKARRQ
                     IDFVDAEWNVLKLLRDEDTAAFLQARLDARYGHVLLDEFQDTNPLQWQILLAWLAAYS
                     DASRPTVFLVGDPKQSIYRFRRAEPRLFSVAADFLMREFGAERCEQDATRRNAQPIID
                     VVNAVFIDQPAFSPFREQTSLAGKLSGRVELMPLFANAEGEGSTVREVASALRNPLLE
                     PDVEPEDTRLRMEAECLGAKIGEMVGFWQIDEKRVDGTSRQRPLNYGDIMLLVRTRTK
                     LSYYERALSAANIPFAAGSRGGLLSALEVRDCVALLEFLVTPMADLKLAHALRSPVFA
                     CSDDDLMQLAARQEPSWWQRLQTLAGEGSASPCLARATRLLGDWLQAADRLPAHDLLD
                     RIFHQGEVLARYRLAAPEATSAGVEANLRALLLLALDVDGGRYPSLPRFIDELRDLSE
                     AEANDAPDEGGIESRDIDAGRVRILTIHSAKGLEAPVVWLLGANSGPRSQEAWDVLVD
                     WQPQAASPQHFSFFGRKEDHGVARQPLFDAESTAAKREELNLLYVALTRARQVFIASG
                     IENTKERGGTPYRLLQAALEKLAKPGDGLAYGDNLPLTTVAPAEVSSSIAQVARAPFP
                     RVGERRHLPDAAARFGILLHALLERRTGGEVSAGWWTELGFDDDEYRCVLPVAERLLA
                     AEALQRFFDPTKYRRAWNEVELTTGEGQLQRIDRLVEFDSAFWVLDYKSSGIDSLRLE
                     EYREQIATYCSAVKSVFSGRQVRGALIFSDASLLEVC"
BASE COUNT         1119 a         1639 c         1457 g         1024 t
ORIGIN      
        1 aatctcatca ctgaaactcg gtcaacaaat ccgctcgagg aatttgcgcg tcgcagaaca
       61 aacagccccg acaggctccg ttttctccag tcaaactcaa cgcgacggaa acgctgcacg
      121 accggactta cccacagaaa ctgtcgataa acctgtggaa ttgctgtgcg cccaccagga
      181 caaaccctgt gccggggaag gtcgcaagaa attgcccaat tgatgtgcaa cgcagtctcc
      241 gcgatggcag ggtcaagcgg cgttcgatgc ctcgtcacgc aactcgcgcc gcagaatctt
      301 gccgacattg ctcttcggca actccgcccg gaattcgacg tggcttggca ccttgtacgc
      361 ggtgaggtac tcgcgacaat gcgcgacaag ctgttccgcc gtcagagtct gatctttcct
      421 gattacaaag atcttgaccg cctccccgga tctctcgtcc ggtacgccga tcgcggcaac
      481 ctccattacc cccggatgca tggccaccac attttcgact tcactcggat agacattgaa
      541 tccggagacg agaatcatgt ccttcttgcg gtcgacaatt cgcacgtaac ccttttcgtc
      601 catgaccgcg atgtcgccgg ttcgcaaaaa gccatcctgc atgagaacgc acgccgtttc
      661 gtctggccgg ttatagtagc ccatcatcac ctgtggcccg cggatgcaca gctcgccgcg
      721 ttgtccaagc ggtacatctt caccaatgtc attgcgtatc gcgacttccg tcgaggagat
      781 cggcaatccg atcgagccgg tgtagtcaga aatattcagc ggattgatcg ttgccgcagg
      841 cgatgtttcg gtcaggccgt aggcctcgat cagaggtagt ccggtaatgc gtttccattt
      901 ctcggccaca gcctgatgca cggccatgcc tcctcccatc gcaagcttca gtttgctgaa
      961 atccagcgcg ttgaaatctt cgttgccgag cagtgcattg aacaaggtgt taacgcccgt
     1021 gatgaccgta aacggatact tgccaagctc ggcggtaaac gcagggatgt cgcgggggtt
     1081 ggtgatcaac aggttgcttg caccgatctt gatgaaggtg aagcagttcg ccgtcaatgc
     1141 aaaaatatgg tacaggggca acgcagtgac aatgaattcg ccaccgtcag taacgaccgg
     1201 cctgatccag gcatgtgcct gcgccagatt ggccagaacg ttccggtgcg tcaacatcgc
     1261 gcctttggag agaccggtgg taccgcctgt atattgcaga aaggccaggg cctcttgcgt
     1321 cacttgcacc ggggtaaaat cagcgacagc accttttgtc agggcttggc cgaaatggac
     1381 agcgccaggc agcttccacg gagggatcat tctcttgaag tacttgacga cgaaattgac
     1441 gacgcggccc ttcgggaagc cgaacatgtc acccaggcgg gccaccacga tgtgccgaat
     1501 cggtgtcttg tcaatcaccc gttccagcgt atgcgcaaag ttttctagta tgacaatagc
     1561 ttcggcttcg gaatccttga gttgatgctc aagttcgcgt ggcgtgtaaa gcgggttgca
     1621 attcaccacc gtataaccgg cgcgcaaggc tcctagcatg cagatcggat actgaagaac
     1681 gttgggcatc atcagcgcaa tgcgcgaacc caacggcagt ccgagaaccg cctgcaggta
     1741 ggccgcaaaa tcgcgcgaca gtttatcgag ctcgccatag ctcaattcga cgcccatatt
     1801 gatgtaggcg acgcgctcgc gatactgtga aaaactcttc tcgaacatct cgccaagcga
     1861 ctgaaactcg ttcagatcaa cctcggcagg cacgccatcc tgataactct gcagccatac
     1921 cttgtccacc ggctatcctc cgaagcagct tagggaatga tgaatgccgc agcaccacct
     1981 ccgggtgatg ctattccatc cggctagttt tgtgaagaag tgatgttggg ggaaatcagc
     2041 acacttcaag caaggacgca tcggaaaaaa tcaaggctcc acgcacctgc cgtcccgaga
     2101 atacactctt caccgcactg caatacgtcg caatctgctc gcgatattcc tcaagacgca
     2161 gactgtcgat tcccgagctc ttgtaatcga gaacccagaa tgcggagtcg aactcgacga
     2221 gtcgatcaat ccgctgcagt tgaccttcac ccgtcgtgag ctcgacttca ttccacgccc
     2281 ggcgatactt cgtcggatcg aagaagcgct gcaatgcctc agcggcaagc aggcgctcgg
     2341 ccaccggcaa gacacaccgg tattcgtcgt cgtcgaatcc cagttccgtc caccagcctg
     2401 ccgaaacttc cccgcctgtt cggcgttcga gcagagcgtg cagaaggatg ccaaaacgtg
     2461 cggcagcatc cggcaggtgg cggcgttcgc ccacccgcgg gaacggcgca cgcgcaacct
     2521 gcgcaattga tgatgaaact tctgcgggcg caacggtcgt gagcggcaag ttatcgccgt
     2581 acgccaatcc gtctcccggc ttggccagtt tttccagggc tgcctggagc aggcggtagg
     2641 gcgtcccacc cctttccttg gtgttttcga ttccactggc gatgaatacc tgcctggcac
     2701 gcgtcaaggc gacatagagc agattcaatt cctcgcgctt cgccgccgtg ctttcggcat
     2761 cgaacagggg ctgccgtgcc acgccatgat cttccttgcg gccgaaaaag gagaagtgct
     2821 gcggcgacgc tgcctgtggc tgccaatcga ccagcacgtc ccacgcctcc tgcgaacgcg
     2881 gcccggagtt ggctccgagc agccagacaa cgggggcttc caggcccttg gcgctgtgga
     2941 tggtcagaat ccgcacgcgc cctgcatcga tgtcccgtga ttcgatgccg ccttcatccg
     3001 gcgcgtcgtt ggcctcggct tcactcaggt cgcgcaactc gtcgatgaag cgtggcaggc
     3061 tcggataccg cccgccgtcg acatcgagtg cgagcaacag aagcgcgcgc agattggcct
     3121 cgacgcccgc gctggtcgct tccggcgctg ccagccggta acgggcgagc acttcgccct
     3181 gatgaaagat gcggtcgagc agatcgtgcg ccggcaagcg atcagccgcc tgcaaccaat
     3241 cgccaaggag ccgggtcgcg cgtgccaagc agggtgatgc acttccttcc ccggcaagtg
     3301 tctgaagtcg ctgccaccat gacggttcct gccgtgccgc aagctgcatc aaatcgtcat
     3361 cgctacaggc aaaaacgggc gaacgtaaag catgggcgag cttcaggtcg gccatcggcg
     3421 ttacgagaaa ttcgagcagg gccacacagt cgcggacctc cagcgccgat agaaggccgc
     3481 cgcgcgaccc cgcagcgaaa ggtatattgg cggcactcaa cgcccgctcg taatatgaaa
     3541 gcttggttcg agtgcgcacc agcaacatga tgtcgccgta gttgagcgga cgttgccggg
     3601 atgtcccgtc gacccgcttt tcgtcgattt gccagaaacc caccatctcg ccgatttttg
     3661 cgccgagaca ctcggcttcc atccgcaacc gcgtgtcctc cggctccacg tcgggctcaa
     3721 gcagcggatt acgcaaggcg gaggcaacct cacgcacggt ggatccttca ccctccgcat
     3781 tggcaaacag tggcatcagc tcaacccggc ccgacagctt gcccgccaat gaggtctgct
     3841 cccggaaagg cgaaaaggct ggctgatcga taaagactgc gttgaccacg tcaatgatcg
     3901 gttgggcatt gcgccgggtg gcatcctgct cgcagcgctc tgcgccaaac tcgcgcatga
     3961 ggaaatcggc cgccaccgaa aacagccttg gttcggcgcg gcggaagcga tagatcgact
     4021 gtttcgggtc accgaccaga aacacggttg gacgggatgc atccgaatac gctgccagcc
     4081 acgccaacag aatctgccac tgcagcgggt tggtatcctg aaattcgtcg agcagcacat
     4141 gcccgtaacg cgcgtccagc ctggcttgaa gaaatgctgc cgtgtcctcg tcgcgaagca
     4201 gcttgagcac attccactcg gcatcgacaa aatcgatctg tcgccgcgcc ttcttgaact
     4261 cgtccacatg cttgagaaag gcagcaaaca ccgtgcaggc attttggttg aatgcgagga
     4321 tgcgctcagc cgtctgttgt tcaatgcaat cgagcacgcg ttggctcagc gtgccatgca
     4381 gcgccaccag acgcgcggcg ccctctggtg taaaacgctt ctcgcttgct gtattcactt
     4441 cccgcttgcg cacggtattg gtcgttgtca gcaacacttc gcgcaataga tccaggcttc
     4501 tttcccgcac cggctcaagc agccccgcac gcaatatccg gacatatttc tggtcggtgg
     4561 acagctcact cgtttcgagc aggccgaggt aattctgaaa ctccagcgcc caatcgccgg
     4621 caaagaaggc gtccatagcc tgccctggcg caccggcagc aagcagttcg cgtagcgcag
     4681 cgacgacttt ccctgcagtg tccgatttct ctccagcaaa ggccagccac tcgctgcgcc
     4741 gttccagtgc gcgacgaatc atgcgccgcg tctgatccag ccccgcctcg tccaacagcc
     4801 ggacgaagct atgcgtgact tcgctctctg gcagtttctg caggctggac gcgaaggact
     4861 gccagagttc gtcgaacaag cgcgaatcag attcgatcag cgtcgccccg gtcagatttg
     4921 ccgacagcgg tgcggcatcg accagttgca ggaaccagcc gtggaaggta ttgaccgcga
     4981 gccccggctg cgccaccagt acgcgctcgt acaagccgcg agcggcgcgt atcgtcgtac
     5041 cctcgccctc atccactgct agaccgcgct cggagagaaa agcaaggacc tgctcatcgc
     5101 tgccggtggc cagcaggtgc aaccagtcca cgacgcgttc ctcgatctcc cgtgcggcct
     5161 tgcgcgtaaa agtgatcgcc agaatctcgc cgggtgagac gccggccagc agcaaccgaa
     5221 cgatgcgcga ggctagcag
//
LOCUS       DAORPM010000176         5191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4519_length_5191_cov_18.942757, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000176 DAORPM010000000
VERSION     DAORPM010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4519_length_5191_cov_18.942757"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLD53_08800"
     CDS             complement(<1..227)
                     /locus_tag="PLD53_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61052.1"
                     /translation="MLKALFRNAMTVLLLSAVAQTTLADSLTDQAQALLDQGKAKDAF
                     NLLEPLESQRAGDENFDLLFGIAAIDAGQNTR"
     gene            complement(267..1010)
                     /locus_tag="PLD53_08805"
     CDS             complement(267..1010)
                     /locus_tag="PLD53_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRC61053.1"
                     /translation="MMKARFFIVFLLSLIASVCFAQGAASVIFASGNAQIVGKDGRHR
                     AAVRGAEVVVGETVETADGRVQLRFSDGANMSLQPDTRFRIDEFRLVEKSGKASSDDR
                     SFFSLLKGGFRTITGLIGKLRREQYKVDAVVATIGIRGTDYSAQLSESGLTVSTFGGL
                     VEVCTDAGCSQVAPGETVHVTDRKSKPRNEERGTGGALNVPATPALPPPKPIEVLPAV
                     TPPAQTPIQAPSTAPSMPGPSYSIPMTRP"
     gene            complement(1161..3398)
                     /locus_tag="PLD53_08810"
     CDS             complement(1161..3398)
                     /locus_tag="PLD53_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HRC61054.1"
                     /translation="MRFHFPVIIIDEDFRSENASGLGIRALAQAIEEEGMEVLGVTSY
                     GDLTSFAQQQSRASAFILSIDDEEFKGDEAEETITELRAFVKEIRCHNEGIPIFLYGE
                     TRTSRHIPNDVLRELHGFIHMFEDTPEFIGRYVVREAKTYLKSVPPPFFRALTHYAAD
                     GSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVEELGQLLDHTGPVAAS
                     ERNAARIFNADHLYFVTNGTSTSNKIVWHSTVAPGDIVVVDRNCHKSVLHSIIMTGAV
                     PVFLMPTRNHYGIIGPIPKEEFRWENIQRKIEAHPFARDVKTKPRVLTITQSTYDGIL
                     YNVEEIKDMLDGKIDTLHFDEAWLPHAAFHDFYGDYHAIGADRPRCKESMIFSTQSTH
                     KLLAGLSQASQILVQNSEGRELDRNVFNEAYLMHTSTSPQYAIIASCDVAAAMMEAPA
                     GTALVEESIAEALDFRRAMRKVGLDWGADWWFKVWGPDDLSEEGIEEREAWMLKPGER
                     WHGFGMLAEGFNMLDPIKATVITPGLDVDGDFADWGIPAAIVTKYLAEHGVIVEKCGL
                     YSFFIMFTIGITKGRWNTMVTELQQFKDDYDKNQPLWKVLPEFVAKLPRYERIGLKDL
                     CKEIHGIYATNDVARLTTEMYLSNMEPSMKPTDAFAKMAHREIDRVPIDDLEGRITSI
                     LLTPYPPGIPLLIPGERFNATIVRYLKFARDFNERFPGFETDIHGLVKEMVDNRPCYY
                     VDCVR"
     gene            complement(3469..4035)
                     /gene="dcd"
                     /locus_tag="PLD53_08815"
     CDS             complement(3469..4035)
                     /gene="dcd"
                     /locus_tag="PLD53_08815"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRC61055.1"
                     /translation="MSIKSDKWIRRMAEQHGMIEPFESGQVREIEGRKIVSYGTSSYG
                     YDIRCSNEFKLFTNINSTIVDPKNFDPNSFVEVHNDFCIIPPNSFALARTVEYFRIPR
                     SVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGIAQVLFF
                     EADEECETSYKDRGGKYQGQIGVTLPKM"
     gene            complement(4155..>5191)
                     /gene="apbC"
                     /locus_tag="PLD53_08820"
     CDS             complement(4155..>5191)
                     /gene="apbC"
                     /locus_tag="PLD53_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HRC61056.1"
                     /translation="TKKDFVSTRSAKNIKVDGDKVSVDIDLGYPAKTQLESIRDTVIA
                     AISAIPGVANVVANVSFKIVAHAVQRNLKPLKGVKNIIAVASGKGGVGKSTTAVNLAL
                     ALAQEGATVGLLDADIYGPSIPQMLGLAGRQPESEDGTSMDPLQAYGLQTMSIGFMID
                     VDSPMVWRGPMVTQALEQLLNQTSWRDVDYLVVDMPPGTGDTQLTLAQKVPVTGAVIV
                     TTPQDIALIDARKGLKMFDKVGIPILGLVENMSIHICSKCGNEEHIFGTGGGEKMCKD
                     YDVELLGSLPLELSIREMADAGRPTVVGAPDSRAAEIYREIARRVAVRVAERAKDMSG
                     KFPNIVVQNT"
BASE COUNT         1099 a         1485 c         1439 g         1168 t
ORIGIN      
        1 cgtgtgttct gcccggcatc aatggcggca atcccgaaca gcagatcgaa gttctcatcg
       61 cctgcacgct ggctctccag cggctcaagc aggttgaagg cgtctttggc ttttccctgg
      121 tccagcaaag cctgtgcctg atctgtaaga ctatccgcta gggtcgtttg agccactgcc
      181 gacagaagca agacagtcat ggcgttgcga aacagcgctt ttagcattgc cgctccctga
      241 acttcgcatt gtaaaaatac atatcgttac ggcctggtca tcggaatgga ataacttggg
      301 cctggcatcg aaggcgctgt gctcggagcc tgtatcggag tctgtgccgg tggtgtgaca
      361 gcaggcagta cttctatggg cttgggcggc ggcagagcag gcgtagcggg tacattcaac
      421 gcgccgccag tgcctcgctc ctcatttcgc ggtttgctct ttcggtcagt cacgtgcacc
      481 gtctcacccg gtgccacttg cgaacacccg gcatcagtgc acacttcgac cagaccacca
      541 aaagtgctca ccgtcaaacc cgactcgctc aactgcgcgc tgtagtcggt gccgcgaatg
      601 ccgatggtcg ccactacagc atcaaccttg tactgctcac gccggagttt gccgatcagg
      661 cctgtaatcg tgcgaaaccc acctttgagc agcgagaaaa agctgcgatc gtcggaactg
      721 gctttgccgc tcttctcgac aaggcggaat tcatcaattc tgaagcgtgt gtcgggctgg
      781 agcgacatgt tcgcgccgtc actgaagcgc aattgcacac gcccatcggc tgtctcgacc
      841 gtttctccga ccacaacttc ggcgccgcgc actgctgctc ggtgccgccc atccttcccg
      901 acaatctgag cgttcccgct ggcaaagatc acagacgccg ctccttgtgc gaagcagacg
      961 ctggcaatta acgacaacaa gaacacaata aaaaatctgg ctttcatcat gctggattcc
     1021 aacctcgact cgccattaat aatgcgaatg aattcattgc catacaaacg tttcatgaag
     1081 ttaaatgaca acgtcggctc agtcaacaaa aaacggcgtc acaggtcatt gtttggtttg
     1141 gcgtgaaaaa aaacaacagg tcagcgaacg caatccacgt agtagcatgg cctgttgtcg
     1201 accatttctt tgaccagtcc gtggatatcg gtctcgaacc ccgggaaacg ctcgttgaaa
     1261 tcacgcgcga atttcaggta gcgtacgatc gtcgcattga agcgttcacc ggggatcaac
     1321 agcggaatgc cgggtggata cggcgtcagc aggatgctgg tgatgcgccc ttcaaggtcg
     1381 tcgatcggca cccggtcaat ctcgcgatgg gccatcttgg cgaaggcatc cgttggcttc
     1441 attgacggtt ccatgttgga gaggtacatc tcggtcgtca ggcgtgcgac atcgttggtc
     1501 gcgtagattc cgtgaatttc cttgcacaga tccttgagac caatgcgctc atagcgcgga
     1561 agtttggcga cgaattcggg cagcactttc catagcggct ggttcttgtc gtaatcatcc
     1621 ttgaactgct gcaattccgt caccatagtg ttccatcggc ccttggtgat gccgatggtg
     1681 aacatgatga aaaacgagta gagcccgcac ttttcgacga taaccccgtg ttcggccaga
     1741 tacttggtaa caatcgccgc cggaatgccc caatcggcaa aatcgccatc cacgtcgagc
     1801 cccggagtga tcacggtggc cttgatcggg tcgagcatgt tgaagccttc ggcaagcatg
     1861 ccgaaaccat gccagcgttc gcccggcttg agcatccagg cctcgcgttc ttcgatgcct
     1921 tcttccgaaa gatcatccgg cccccacacc ttgaaccacc agtcagcgcc ccagtccaag
     1981 cccaccttgc gcatggcgcg gcggaaatcg agcgcttcag cgatggattc ctcgaccagt
     2041 gccgttccgg ccggtgcttc catcatcgcc gccgctacat cgcatgaagc gatgatggcg
     2101 tattgaggcg aggtcgaggt atgcatcaaa taggcctcgt tgaacacgtt gcggtcaagt
     2161 tctcgacctt ccgagttctg caccagaatc tgcgaagcct gtgacaaacc agccagcaac
     2221 ttgtgcgtcg actgagtcga aaagatcatt gactccttgc agcgtggccg gtcggcgcca
     2281 atggcgtggt agtcgccgta aaagtcgtgg aaagcggcat gcggcaacca cgcctcgtcg
     2341 aaatgcagcg tatcgatctt tccgtcgagc atgtccttga tttcctcgac gttgtaaaga
     2401 atgccgtcgt aggtgctttg ggtgatcgtc aggacgcgcg gtttggtttt cacatcacgc
     2461 gcaaagggat gggcctcgat cttcctctga atgttctccc agcgaaactc ttctttcgga
     2521 atagggccaa tgatcccgta atggttgcgt gtcggcatca gaaacacagg cacggcaccg
     2581 gtcatgatga tcgagtgaag taccgacttg tggcagttgc ggtcgacgac gacaatatcg
     2641 cccggcgcca cggtcgagtg ccagacgatc ttgttcgagg tcgaagtgcc gttggtcacg
     2701 aaatacagat ggtcggcgtt gaagatacgc gccgcgttgc gctccgaagc cgccaccgga
     2761 cccgtgtgat cgagcaattg acctagctcc tcaacagcgt tgcagacgtc tgcgcgcagc
     2821 atgttctcgc cgaaaaactg gtggaacatc tgtcctacgg ggcttttcag aaatgcgaca
     2881 ccgcccgagt gtcctgggca atgccatgaa tacgaaccgt cggcagcgta gtgtgtcagc
     2941 gcgcggaaga agggcggcgg cacgctcttc aggtaggtct tggcctcacg cacgacataa
     3001 cggccgataa actcgggcgt gtcctcgaac atgtgaatga agccatgcag ctcacgcaac
     3061 acatcattcg ggatgtggcg ggaagtgcgt gtttcgccgt aaagaaagat cggaatgcct
     3121 tcgttgtggc agcggatttc ctttacgaaa gcacgcaact ccgtgatggt ttcttcggct
     3181 tcatcgccct tgaactcctc gtcgtctatc gacaggataa acgccgaggc gcgactttgc
     3241 tgctgggcaa acgaggtcag gtcgccgtag ctggttacgc cgagaacttc catcccttct
     3301 tcctcgatgg cttgtgccag ggcgcgaata cccaatccgc tggcattttc cgagcggaaa
     3361 tcctcgtcga tgatgatgac cggaaaatga aaacgcatgg tatgaatccc tgaggagttg
     3421 agggtcagga gagaggagtg gatcgccagg agctctcctc gcgagtcact acatcttggg
     3481 cagcgttacc ccgatctgcc cctggtactt cccgccgcga tccttgtatg aggtttcgca
     3541 ctcttcgtcg gcttcaaaaa acaggacttg tgcgatacct tcattggcgt aaatcttggc
     3601 cggtagcggc gtggtattcg agaattcgag cgtcacgtag ccttcccatt cgggctcaaa
     3661 aggggtgacg ttaacgataa tgccacaccg tgcgtaggtg ctcttgccca gacagacggt
     3721 gagtacgctg cgcgggatgc ggaagtattc gaccgtgcgc gccagagcaa acgagttcgg
     3781 cgggatgatg caaaaatcgt tgtgcacttc gacgaaagaa ttcgggtcga aattcttggg
     3841 atcgacgata gtcgaattga tgttggtgaa cagtttgaat tcgttcgagc agcgaatgtc
     3901 gtagccgtag ctcgacgtgc cgtaggaaac gatcttccgg ccttcaatct cgcgaacctg
     3961 gcccgattcg aacggctcga tcatgccgtg ttgctccgcc atgcggcgga tccacttgtc
     4021 ggatttgatg gacatctgcg atcacccgga aaccgttatg cgaaggcggg tattttacgg
     4081 tttccgcaga tttcagggaa attgctttgc ggctccaatg aatccggagt ccgggtgttt
     4141 cgcagaatgt ctcgtcacgt attctgtacc acgatattcg ggaacttgcc gctcatgtcc
     4201 ttggcgcgtt ccgccaccct gacggcaacg cggcgggcaa tctcccgata aatctctgct
     4261 gctctggaat ccggggcgcc aaccaccgtc ggtcggccgg catcggccat ttcgcgaatc
     4321 gatagctcaa gtggtagcga gccaagcaat tcgacgtcgt aatccttgca catcttctcg
     4381 ccgccgccag tgccgaagat atgctcctcg ttgccgcatt tcgagcagat atggatgctc
     4441 atgttttcaa ccagaccaag gatcgggatg ccgaccttgt cgaacatctt cagccccttg
     4501 cgcgcgtcga tcaaggcgat atcctgcggt gtggtgacaa tcacggcacc agttaccgga
     4561 accttttgcg ccagcgtcag ttgcgtgtcg ccggtgcctg gcggcatgtc gacgacaaga
     4621 tagtccacgt cgcgccaact ggtctgattg agcagttgct ccagcgcctg tgtgaccatc
     4681 ggcccgcgcc agaccatcgg cgaatcaacg tcgatcatga agccgatcga catcgtttgc
     4741 agtccgtagg cctgtagcgg gtccatcgac gttccatcct ccgattccgg ctgacgaccg
     4801 gccagaccaa gcatctgcgg tatggacggg ccatagatgt cggcgtcaag cagccccacc
     4861 gttgcccctt cctgcgccag cgccaaggcc agattgaccg ccgtcgtgct tttgccgacc
     4921 ccgcccttgc cagaggctac agcgatgatg ttctttacgc ccttaagcgg tttcagattg
     4981 cgctgcacgg cgtgcgcaac gatcttgaag ctcacattgg cgacgacgtt ggcgacgcct
     5041 ggaatggccg aaattgccgc aatcaccgta tcccgaatgc tttcaagctg agttttcgcg
     5101 gggtagccaa ggtcgatatc cactgacacc ttgtcaccat cgaccttgat gttctttgcc
     5161 gagcgggtcg aaacgaagtc cttcttcgta t
//
LOCUS       DAORPM010000177         5189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4520_length_5189_cov_13.022010, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000177 DAORPM010000000
VERSION     DAORPM010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4520_length_5189_cov_13.022010"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="mnmH"
                     /locus_tag="PLD53_08825"
     CDS             <1..847
                     /gene="mnmH"
                     /locus_tag="PLD53_08825"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HRC61057.1"
                     /translation="GAAYVSANIALHLRETFRDRPKTWRPLIVCWRGGQRSGAMTYVF
                     RRIGWDAQQLKGGYKSYRKQVVATLAELPRRLSFNVVCGATGSGKSRLLQALARRGEQ
                     ILDLEELACHKGSVLGVLPDLRQPSQKMFETRLLATLQRLNPERPVYVEAESRKIGTI
                     QIPNALLETMRAGSCIDIEASLEARVDFLLGDYDYFLKSPDWLNSLLDELRKLQSRET
                     IARWQAYANCAQWRTLVYELLQQHYDPLYHRSQVRNFTRIGASRNFIANDLTPAGIDA
                     LVRQI"
     gene            970..3663
                     /locus_tag="PLD53_08830"
     CDS             970..3663
                     /locus_tag="PLD53_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HRC61058.1"
                     /translation="MLAQHYLNTLFEPKSVAVIGASDRENSVGHFLFKNILESGYKGR
                     LYPINTKHEIVQSVLAYKSIEEIGARVELAIIATRAQTVPNIVEQCGRCAIRNIIIIT
                     SGFAEAGHSGAALERKTLEIARRYNVRVLGPNCLGIIRPELGLNATFLRVSANVGNLA
                     LVSQSGAICSAVLDWAKSNQVGFSSVISLGSTADVDFGEILDYLVYDNRTHYILLYIE
                     GIRNSRRFMSALRSAARIKPIILLKAGRHAAGSAAVKVHSGMLAGSDTVFDAAVKRSG
                     VVRVQNIGQLFYASKVLSSKFRPLGKRLAIITNGGGPGAMAADRAGDMVIPLAELSTD
                     TLKALNAKMPASWSQSNPIDIRGDATPERYRDAILTVAQDDGVDCILVMLAPQAMTEP
                     MAVAQVLIEVSEQAPKPIIACWMGKDQVAEGRRALENAGIPAFHTPETAVELFYHIST
                     YYRNQTLLLQTPEGSSKRTQQETEGAKMLIEAVLQENRKVLSEMESKAVLRAFRIPVA
                     ETMVAHTPTESILLAEQIGFPIAMKVDSPDLIRKTEAGGVRLNITNAPAVRNAYHDII
                     ETVQKKHPTARIGGVSIEPYVARPNGRELMIGVVRDPTFGPIITFGAGGSEVEIFSDR
                     AVALPPLNRFLAQDLIQSTHAAQLLGEFRGKPAINMKALEEVLLNISEMVCELPWLLE
                     LDLNPLIVDEFGAIAADARIVIDYAATTSDRYAHMAIHPYPVHLIQDWEQRDGSIITI
                     RPIRPEDADMEQSFVKNLSDESKYFRFMDTLRELTQSMLVRFTQIDYDREMALIATVP
                     GDDGNEVQIGVARYVTNPDGETVEFAVAVADDWHKRGIGRKLMNALIEIARQKGYRAV
                     IGDVLAQNARMLRLMTTLGFTIHPHHEDTAVKRVVCPLHNQ"
     gene            complement(3805..4002)
                     /locus_tag="PLD53_08835"
     CDS             complement(3805..4002)
                     /locus_tag="PLD53_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HRC61059.1"
                     /translation="MAIYESEHTKFIREKLMKHPEWAEDQLVGRALLWDRKIDLNEQK
                     EIRESSESNRPYPYDVNFDQD"
     gene            complement(4073..4306)
                     /locus_tag="PLD53_08840"
     CDS             complement(4073..4306)
                     /locus_tag="PLD53_08840"
                     /inference="COORDINATES: protein motif:HMM:NF023513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 family protein"
                     /protein_id="HRC61060.1"
                     /translation="MNIVYNSESYAIVAYPVQQGFELVDKLGCRTLFVQGSVATDLRD
                     AIDSIPYEERNEESIDSLLDEYCVGATRPIMYH"
     gene            complement(4467..>5189)
                     /locus_tag="PLD53_08845"
     CDS             complement(4467..>5189)
                     /locus_tag="PLD53_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC61061.1"
                     /translation="AAYIAGGLAVLAFGLLFALRRDLDDEQNPSGTGKLRADRVAVST
                     LSVLSSPAVLLCFVFFFLITAAFGALQNFSVAALKAMYGLSLATAASCLSAYLLGGAV
                     GIAVGGFVASRRAQHKIIALCLSFGALISLLLASNFVVGWIVLPLMAVMGFGVGIAGP
                     SRDLLVRKAATQGLGEASFGRVYGFVYSGLDIGLATAPLIFGPIMDGGHFGAMWVGVA
                     LLQGLAVVSALRVGSRADCDRV"
BASE COUNT         1238 a         1512 c         1357 g         1082 t
ORIGIN      
        1 tggcgcagcc tatgtctcgg caaacatagc gctccacctg agagaaacct tccgtgatcg
       61 gcccaagacc tggcgaccac tgatcgtctg ctggcggggc gggcagcgca gcggtgcgat
      121 gacctatgtc ttcagacgca ttggctggga tgcccagcaa ctcaagggcg gctacaagag
      181 ttatcggaag caggtcgtcg cgactctcgc cgaattgccg cgccggctaa gcttcaatgt
      241 cgtctgcggc gcaaccggta gcggcaagag ccgcctgctt caggctttgg cgcgacgcgg
      301 tgagcagatt ctcgacttgg aagaactggc ctgccacaag ggttctgtgt tgggagtttt
      361 accggatttg cgacaacctt cgcagaagat gttcgagacc aggctgctcg ctacgctgca
      421 acgcctgaac ccagaacgtc cggtgtacgt cgaggccgag agccggaaga tcggcaccat
      481 tcagatcccc aacgcgctgc tggagaccat gcgagcgggg tcatgcatcg acatcgaagc
      541 gtctcttgag gcgcgtgttg atttcctgct gggtgactac gactatttcc tgaagtcccc
      601 tgactggctc aattcgctgc ttgacgaact gcgcaaactg caaagccgtg aaaccatcgc
      661 gaggtggcaa gcgtatgcga actgcgccca gtggcgaacg ctggtttacg aactactcca
      721 gcaacattac gatccgcttt atcatcgctc ccaggttcgg aacttcaccc gcattggcgc
      781 ctcgagaaac ttcatcgcca acgacctcac ccctgcgggt attgacgctt tggttcgcca
      841 gatatagggc tcgtgaccga acaagaagtc gaactgaaac gcttttccct acaattgccg
      901 aacgttccat gataacttga cactgtgcat caacaaaccg ttacaacctc tcaatcagat
      961 tgatttttca tgctcgcaca acactatctc aacacgcttt ttgaacccaa atcagttgcc
     1021 gtcatcggcg cttctgatcg tgagaattcg gtcggtcatt ttctgttcaa gaacatcctt
     1081 gaatcaggtt acaaagggcg tctctatcca atcaatacga agcacgaaat tgttcagagc
     1141 gtgttggcgt ataagtcgat cgaagaaatc ggggcgcgcg tcgaactcgc cattatcgcc
     1201 acccgagcac aaaccgtccc caatattgtc gagcagtgcg gccgttgcgc tatcaggaac
     1261 atcattatca tcacctcagg ttttgcggaa gcaggtcact cgggcgcagc gctggagcga
     1321 aaaacactgg agattgcccg ccggtacaac gttcgtgttc tcggccccaa ctgtctaggg
     1381 attattcgcc ccgaactggg tctcaacgca accttcttgc gtgtcagtgc caatgttggc
     1441 aatctggcac tggtttcgca gtctggggcg atttgctctg ctgttcttga ctgggcaaaa
     1501 agtaaccagg tcggcttctc cagtgtaata tcgctcggca gtaccgccga tgtcgatttc
     1561 ggagagattc tcgactacct ggtttacgac aaccgcacgc actacatcct gctctatatc
     1621 gaaggcatac gaaactcgcg acgattcatg agcgcgcttc gctctgcggc acggataaag
     1681 ccgattatct tgctcaaggc gggacgccac gctgcgggct cggctgcggt aaaggtgcac
     1741 tcgggaatgc tcgccggatc cgacaccgtt tttgatgctg cagtcaaacg ctccggcgtg
     1801 gtccgtgttc aaaacatcgg acagcttttt tatgcgtcga aagtactgtc gtccaaattc
     1861 cggccactgg gcaaaaggct ggcgatcata accaacggtg gcgggccagg cgccatggca
     1921 gcagaccgtg caggcgatat ggtcattcca ctggccgagc tttcaactga cacccttaaa
     1981 gcgcttaacg caaagatgcc agccagctgg tcgcagagca atcctatcga tattcgtggc
     2041 gacgcaacgc ccgagcggta ccgtgacgcg attcttaccg ttgcgcagga cgatggagtt
     2101 gactgcatac tggtcatgct tgcaccacaa gccatgaccg aaccaatggc ggtagctcaa
     2161 gtgctgatcg aagtcagcga gcaggccccg aaacccataa tcgcttgctg gatgggcaag
     2221 gaccaggttg ctgaagggcg tcgtgcgcta gaaaacgcag gaatcccggc gttccacaca
     2281 cccgaaaccg cagttgagtt gttttaccac atctcgacgt actaccgaaa ccagacgcta
     2341 ctcctacaaa cgccagaagg ctcatccaaa cgcactcaac aggaaactga gggcgccaaa
     2401 atgctgatcg aggctgtatt gcaggaaaac cgcaaggtgc tgtctgaaat ggagtcaaaa
     2461 gccgtattgc gtgccttccg catacctgtg gcagagacca tggttgccca tacgccgacc
     2521 gagtcgatat tgcttgccga acaaattggc ttcccgatag ccatgaaagt cgattcgccg
     2581 gatctcatac gcaagaccga agcaggcggt gtacgactca acatcaccaa tgccccggcg
     2641 gttcgtaacg cctatcatga cattatcgaa acggttcaga aaaagcatcc gacggcgcga
     2701 atcggcggtg tctcgattga accctacgtg gctcggccga atggccgtga actgatgatc
     2761 ggtgtcgtgc gcgatccgac cttcggcccg atcataacgt ttggtgcagg tggcagtgaa
     2821 gtcgaaatat tcagtgatcg tgctgttgcg ttgccgccac tcaaccgctt tctggcacag
     2881 gatcttatcc agtcaacaca cgccgcccag ttgctcggcg aattccgtgg aaaaccggcg
     2941 ataaacatga aagcacttga agaagtgctg ctcaacattt ccgaaatggt ctgcgaactg
     3001 ccctggttgc tggagcttga cctcaatcca ctgatcgtcg acgagtttgg cgccatcgcc
     3061 gctgacgcgc gaatagtcat cgattacgcg gcgacgacca gcgaccgtta tgcgcacatg
     3121 gccattcatc cctaccccgt gcatctgatt caggactggg aacagcgtga tggaagcatc
     3181 atcaccatcc gcccgatccg ccctgaagac gccgacatgg agcagtcatt tgtcaagaat
     3241 ctctcggatg aaagcaagta ttttcgattc atggacaccc tgcgcgaact cacgcaatcc
     3301 atgctggtgc gttttacgca gatcgactac gaccgcgaaa tggccctgat tgccactgtt
     3361 ccaggcgacg atggcaatga ggtgcagatc ggtgtggcgc gctacgttac caacccggac
     3421 ggggaaacag tggagtttgc tgtggctgtt gccgacgact ggcacaagcg cggcatcgga
     3481 cgcaaattga tgaacgcgct gatcgaaatc gcacgccaga agggttaccg ggcggtcata
     3541 ggcgacgtac tggcacagaa tgcccggatg ctccggctga tgaccactct cggtttcacg
     3601 attcatccgc accacgaaga tactgcggtc aaacgcgtcg tctgcccact gcataaccaa
     3661 tagtcgtaga ccggaagcgt ctgccactca gaagaacggc cagacgaatg tctggccgtt
     3721 cttgtcaatt cgaatgcatt ccgatttcaa ggagcaccgc agaacaggca ccttgatttt
     3781 ctagcgaatg gaataggccc gatatcaatc ctgatcgaag ttcacatcat aaggataggg
     3841 ccggttcgat tcgctcgact cacggatctc cttctgttca ttcaggtcga tctttctgtc
     3901 ccacaacaac gcgcgcccta cgagttggtc ttcagcccat tccgggtgct tcatcagctt
     3961 ctcgcgtatg aatttagtat gttccgattc gtatattgcc atttgctact ccgaacaaca
     4021 ggggtttagg taagcgcgac gggcgcggtg cgtccattgt acccctgtgc tttcagtgat
     4081 acatgatcgg tcgcgtcgca ccgacacaat attcgtcaag aagcgaatca attgattcct
     4141 cgttccgctc ttcgtaagga atactgtcga tcgcatcacg cagatccgtc gccaccgaac
     4201 cttgaacgaa aagagtccgg caacccagct tatcgaccaa ctcgaatccc tgctgcaccg
     4261 ggtaggcaac aatggcatag ctttcgctgt tgtagacaat attcatgacc actcctctgc
     4321 tcagggtcgg cacctgctcc gcgacacatg tctcgaagcc ctgctaatcc tacccgttca
     4381 actgtgacgg gagatgcgtc cgttttgttc catttcaaga ccgcaggcca cactatatga
     4441 gcttcgagtc ggcaatgttc caaaaatcat acccgatcgc aatcggcacg gctccctaca
     4501 cgcagggctg agacaacggc taatccttgc agcagggcaa caccgaccca catggcaccg
     4561 aaatgtccgc cgtccatgat cgggccgaaa atcaaagggg ccgttgccag cccgatatcc
     4621 agacccgaat agacgaaacc gtacactctg ccgaacgatg cctcaccaag cccttgcgtt
     4681 gccgccttgc gcaccagcag atcacgtgac gggccagcga ttccaacacc aaaacccatt
     4741 actgccataa gtgggagcac tatccagccc accacgaaat tgctggcgag caagaggctg
     4801 atcaaggcac cgaaggagag gcatagcgcg atgatcttat gctgcgcgcg cctgctggcc
     4861 acgaaaccac cgaccgcgat accgaccgca ccaccaagca gatacgcaga caggcaggag
     4921 gcagcggtcg ccagagacag tccatacatc gccttcaatg ctgcgaccga gaaattctgc
     4981 aaggctccga aagctgccgt aatcaggaag aagaagacaa agcaaagcaa caccgctggc
     5041 gagctcaata cactgagtgt cgaaacggct acgcggtctg cccgcagctt cccggttcca
     5101 ctggggtttt gctcatcgtc aagatcccgt cgcagcgcga aaagcaggcc gaaagccagc
     5161 acagcaagcc ctccggcaat atacgcggc
//
LOCUS       DAORPM010000178         5185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4525_length_5185_cov_11.695906, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000178 DAORPM010000000
VERSION     DAORPM010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4525_length_5185_cov_11.695906"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..709)
                     /locus_tag="PLD53_08850"
     CDS             complement(131..709)
                     /locus_tag="PLD53_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC61062.1"
                     /translation="MTRLTRLALIAAAMTSLSGPTLAAPETFIIDGTHSFPRFSYNHF
                     GYSKQLSRFNKTSGKIVLDKEAKTGSVEVLIDMKSVDTGYPVFNEHIQGEGLLDTEKY
                     PTASFKSTKVVFQGGKPVAVEGKLTIKDVTRPVTLTITSFQAMPHPMLKKDAIGANAT
                     TKISRSEFHAGKHAPSVGDEVTIDIAVEAIKE"
     gene            complement(756..1328)
                     /locus_tag="PLD53_08855"
     CDS             complement(756..1328)
                     /locus_tag="PLD53_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRC61063.1"
                     /translation="MNSRIILALLSLTTALAWPIHGYADEFNRVQADKSTLVFAFKQM
                     GVGLEGRFKKFTVRLNFDPAKANSAKASLEVDIASIDTGSTEGDDEVAGKLWFNTKAF
                     PTARFESSSVRSLGGNRYEVAGQLTIKGRTQAVTAPATVSIQGNNASFDGAFVIRRAD
                     FAIGEGAWADFGTVANEVQIRFHILATSGK"
     gene            complement(1351..1887)
                     /locus_tag="PLD53_08860"
     CDS             complement(1351..1887)
                     /locus_tag="PLD53_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRC61064.1"
                     /translation="MKRYTITAIGLHWLMAAALFSMFALGLYMADLPLSPEKLKLYSW
                     HKWAGVTVFLLAVMRLIWRVTHRPPDLPDRMSRVEQLIARAAHGLLYLLMFIIPLSGW
                     LMSSAKGFQTVLFGVLPLPDLLAKNKALGDMLETVHWGLNVLLAAVVVGHTAAAFKHH
                     FIDRDDVLTRMLPHHGPR"
     gene            complement(2104..3261)
                     /locus_tag="PLD53_08865"
     CDS             complement(2104..3261)
                     /locus_tag="PLD53_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61065.1"
                     /translation="MRARFAYGLVAATLFFATELVAADFGSKDGVAPANIDEITEILR
                     QDPYDLELLISFGTSKGGSAGHLALAVRDGVPGDDLVYSANFYADRSDEHASGFYTAD
                     LMLGIPKKEYLFKTSSSLAETAAFGLDFGEIYKRSVIGVRVYGVPADEKTAVTDFFQR
                     VNADYHRQAEDTEYHDGEVKYDYMRLNCAKTIGAAFRFGAGYEHLEVTSARLLKRRRI
                     VVAATANIPMEMAMKLIKEWGARGYAMDVVLYKKYDGSTFVDPHDEPPVAFKDLPNRF
                     PSVLSRDFRRDAGEYEDFDNLYAMHLLYSLGRYRVQVNEQTLLLEVKQAKNTKPYAEA
                     VELATAGARSDMENNRRKLLFRSKGKYFWESADNSHLYDFTEDGSSQPSRE"
     gene            complement(3288..4127)
                     /gene="ppk2"
                     /locus_tag="PLD53_08870"
     CDS             complement(3288..4127)
                     /gene="ppk2"
                     /locus_tag="PLD53_08870"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRC61066.1"
                     /translation="MAHKKTRNESTVEEPKAAPLTNKEYAKELKQLHVELVKLQQWVV
                     AKGLKVCIVFEGRDGAGKGGTIKAITERVSPRVFRVVALPAPTEREKSQMYLQRYMAH
                     FPAAGEVVIFDRSWYNRAGVERVMGFCKPELTKKFLDAAPMVERAMVESGIILLKYWL
                     EVSPEEQERRLRDRIHDGRKTWKLSPMDVQSFNRWDDYTQARDDMFAATDSAWAPWFV
                     ALSEDKKRVRLNVISHLLKQVPYKAMPAKKVTLPKRKVSRTTTSDYPFKYIPEHLPVQ
                     GDS"
     gene            complement(4271..4735)
                     /locus_tag="PLD53_08875"
     CDS             complement(4271..4735)
                     /locus_tag="PLD53_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61067.1"
                     /translation="MNKRALWIAVSVAAMVGDASAQQYVYPANGQSQQQQQSDEASCY
                     SWAVQQSGFDPAKPPPQQAAAQPPTTATGTTPGAGARGAVRGAIVGGALGGDAGTGAA
                     VGAVAARGQSRRQNAAAAQQGQQQQQAATQQQNASFGKARAACLEGKGYTVK"
BASE COUNT         1015 a         1473 c         1522 g         1175 t
ORIGIN      
        1 tctacgccgg tatcgcggat gatacggaat cgggcttcgc cgatgccgcc cgccgtgttg
       61 ccctggaaac acgcgatacg atcaaccgct atcgctgatc agccaggcac gttccacgag
      121 caacagtaag ctattccttg atcgcttcga cagcgatgtc gatggtcact tcatcgccca
      181 cggagggagc gtgctttccg gcgtggaact cagagcggct gattttggtg gtggcgttgg
      241 cgccgatggc atccttcttc agcatcggat gtggcatcgc ctgaaatgaa gtgatggtca
      301 gggtcactgg ccgggttaca tccttgatgg tcagcttgcc ttcaaccgcc accggttttc
      361 cgccctgaaa aaccaccttg gtcgacttga aggacgcggt gggatatttc tccgtgtcga
      421 gcagaccttc gccctggata tgttcgttga ataccgggta gccggtgtct acggatttca
      481 tgtcgatgag cacctcaacg gaaccggttt tggcttcctt gtcgagtacg atcttgccgc
      541 ttgtcttgtt gaaacggctg agctgtttgg agtagccgaa gtggttgtag gagaagcggg
      601 ggaagctgtg cgtgccgtcg atgataaagg tttccggggc ggccagagtc gggccgctga
      661 gtgaggtcat ggctgcggca attagtgcga gacgggtaag tcttgtcatg ttatggctcc
      721 ttgctggttt gaggtcgaaa cagggttttg aacgttcatt tcccgctggt ggcgaggatg
      781 tggaacctga tctgaacttc attggcaacg gttccaaaat ctgcccaggc tccttcaccg
      841 atagcgaaat cagcgcgcct gatcacgaag gcaccatcaa aactggcgtt gttgccctga
      901 atgctcaccg tagccggcgc ggttacggcc tgcgtgcggc ccttgatggt gagttggccc
      961 gctacttcgt aacggttgcc gcccaacgac ctgacgctgc tggattcaaa gcgggcggtg
     1021 gggaatgcct tggtattgaa ccatagttta ccggccactt cgtcatcacc ctcggtcgag
     1081 ccggtgtcga tgctggctat atccacttcc agactggcct tggcgctgtt tgccttggcc
     1141 ggatcgaaat tgagccgtac ggtaaatttc ttgaagcggc cttccaagcc gacgcccatc
     1201 tgtttgaagg caaagacgag ggtgctcttg tcggcctgca ccctgttgaa ctcgtctgca
     1261 tagccgtgga tcggccaggc gagtgcggta gtgagtgaaa ggagggcaag aatgatcctg
     1321 ctgttcatga tcggttctcc gttggtgaaa tcagcgcggg ccgtggtgcg gcaacatgcg
     1381 ggtcagcacg tcgtcccggt cgatgaaatg gtgcttgaaa gcggcggctg tgtgaccaac
     1441 tacgacggcc gcaagcagca cgttaagtcc ccagtgaacc gtttcgagca tgtcgcccag
     1501 cgccttgttc ttcgccagca gatcgggcaa ggggagtaca ccgaagagca cggtctggaa
     1561 acccttggcc gaactcatca gccaacccga aagcgggatg atgaacatca gcagataaag
     1621 aaggccatgc gccgcccggg cgatgagctg ctcgacgcgg ctcatacggt cgggaagatc
     1681 cggagggcgg tgcgtcacgc gccagatcag gcgcatgacg gcgagcagaa agacggtaac
     1741 gccggcccat ttgtgccagg agtagagctt cagtttctcg ggtgacagcg gtaggtcggc
     1801 catatagaga cccagagcaa acatgctgaa cagggcggcg gccatcagcc agtgcaggcc
     1861 aattgcggta atggtgtatc gcttcatggt ggggcggatt gctgaaatga gttgatcatc
     1921 aagttgacga ttgcgttgcc tcgatcgtta gaaccagggc gcactttaaa ggtttcaact
     1981 gccgctttac agagggtaat ccggaattga ttgttgcagt ctgcgcaaca aaaatgatga
     2041 gcttccctat actctcgctc ccccgtccga ctacccttga acagcgaaat ttcagggtgt
     2101 cgctcactcg cgcgaaggct ggctactgcc gtcctcggtg aaatcataga ggtgcgaatt
     2161 gtcggcagat tcccagaagt acttaccctt cgagcggaac agcagcttgc ggcggttgtt
     2221 ttccatgtcc gaccttgcgc cggcagtcgc cagctcaacg gcttccgcat agggtttggt
     2281 gttcttcgcc tgcttgacct ccagtagcag ggtctgttcg ttcacctgca cgcggtacct
     2341 gcccaggctg tagagcagat gcatcgcata gaggttgtca aaatcttcgt actcccccgc
     2401 gtcacgacgg aagtcccgcg acagcacgga gggaaaccgg ttaggcaaat ccttgaacgc
     2461 caccggtggc tcatcatgcg gatcgacaaa ggtggagcca tcgtatttct tgtacagcac
     2521 gacatccatg gcgtagccgc gtgcgcccca ctccttgatg agtttcattg ccatttccat
     2581 cgggatattg gccgttgcgg cgacgacgat ccggcggcgc ttgagcagcc tggcgctggt
     2641 cacttcaagg tgttcatagc ccgccccgaa gcggaaggcc gcgccaatgg ttttggcgca
     2701 gttgaggcgc atgtagtcgt atttgacctc gccatcgtgg tactcggtat cctcggcctg
     2761 ccggtgatag tcggcgttga cgcgctgaaa aaagtccgtc accgccgtct tctcatcagc
     2821 cggtacgcca tacacccgca caccgatgac cgagcgcttg tagatttcgc cgaaatcgag
     2881 gccaaacgct gctgtttcag caagcgagga ggaggtcttg aacagatact ccttcttcgg
     2941 gatgccgagc atgaggtctg cggtgtagaa gccggaggcg tgctcgtctg agcggtcggc
     3001 atagaaattc gccgaataca ccagatcatc ccccggcacc ccatcgcgta cggccagtgc
     3061 gagatggccg gccgaaccgc ccttggacgt tccgaagctg atcagcaatt ccaggtcgta
     3121 gggatcctgc ctgagaattt cggtgatttc atcgatattg gcaggcgcga cgccatcctt
     3181 gcttccaaaa tcggcggcga ccagctccgt cgcaaaaaac agagttgccg ccaccagccc
     3241 gtacgcaaat cgcgccctca tcgtcctcac ttggctctac cgcttcttca cgaatcgccc
     3301 tggaccggca aatgctcggg gatatacttg aagggatagt cggaggtcgt ggtgcgcgac
     3361 actttgcgtt tgggtagcgt caccttcttg gctggcattg ccttgtacgg cacctgcttc
     3421 agcagatggc tgatcacgtt caggcgcacg cgcttcttgt cttccgaaag tgccacgaac
     3481 catggtgccc aggctgaatc cgtggcggcg aacatgtcgt cacgggcctg tgtgtagtca
     3541 tcccagcggt tgaacgattg cacgtccatg ggcgatagct tccatgtctt gcgaccgtca
     3601 tggatgcgat cacgcaggcg gcgctcctgt tcttccggac tcacttccag ccagtatttg
     3661 agcaggatga ttccggattc gaccatcgcg cgctcgacca tcggcgccgc gtcgaggaac
     3721 ttcttggtca gctcgggctt gcaaaagccc atgacccgct cgacgccagc gcggttgtac
     3781 cagctgcggt caaagatgac gacctcgccc gccgcaggga aatgcgccat gtagcgctgg
     3841 aggtacatct ggcttttttc gcgctcggtc ggcgcgggca gggcgactac acggaagacg
     3901 cgcggactca cccgctcggt gatggctttg atcgtgccgc ccttgccggc gccgtcacgc
     3961 ccttcgaaga cgatgcagac cttgagccct ttggcgacca cccattgctg caacttgacg
     4021 agttccacgt gcagctgttt gagttccttg gcatactctt tgttggtcag cggcgcggct
     4081 ttgggttcct caacagtcga ttcgtttctc gtcttcttgt gagccatgct tatctccatt
     4141 catgcgtaag gaaaaccggt gatcttcgat cgatatttgc agtgccaaat acacccgata
     4201 ccgggcatgt gcgctccctt gagctgcgca gaacgatgct gaatacaacg gcctgactat
     4261 agaagcacgc ctactttacg gtatacccct ttccttcgag gcatgccgcg cgggccttgc
     4321 cgaaggatgc attctgctgt tgcgtagccg cctgctgttg ctgctgcccc tgctgtgcgg
     4381 ctgcggcatt ctgccggcgg ctctgtcctc gagcagccac cgcgcctact gcagcacctg
     4441 ttcctgcatc tccgcccaag gctccaccga caattgcgcc acgcacagcg cctcgcgctc
     4501 ctgcgcccgg cgtcgtgccc gttgcggttg tcggtggctg tgcggctgcc tgttgcggag
     4561 gtggcttcgc aggatcgaac ccgctttgct gaacggccca tgaatagcag gaagcctcgt
     4621 cactctgctg ctgctgttgg gactgtccgt ttgcggggta cacgtattgc tgtgcgcttg
     4681 catcaccgac cattgctgca accgacacag ctatccagag ggctcgcttg ttcatgttcg
     4741 atttctccaa aaagatttga cacagagcag ggcgacaatt aatcgcaatc ctctccgcag
     4801 gcatacgcag cttcgagtaa ctcaggtatg gcccatttcc tcacatcaag gctgcttatt
     4861 tgatgtaacc gcgattcagc atgagttagt cgccgaactc ggttgcagca aaggttccga
     4921 ggcggcaagt tcagcaccat gccaatattg tacgtcgcta cgcgagattt atgaacggct
     4981 aatattgcat atggcaagcc gggtccagcg taaatccggt gcgtagtggg agaggctttc
     5041 ttgtaggtgc gcgagagcga gctgcagccc ggcaaatgaa aaggccccca tctctggagg
     5101 cctcggtgat actggtgggc ccgcacggat ttgaaccgtg gaccaaagga ttatgagtcc
     5161 tctgctctaa ccaactgagc tacag
//
LOCUS       DAORPM010000179         5107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4642_length_5107_cov_13.263064, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000179 DAORPM010000000
VERSION     DAORPM010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4642_length_5107_cov_13.263064"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..493
                     /locus_tag="PLD53_08880"
     CDS             236..493
                     /locus_tag="PLD53_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61068.1"
                     /translation="MVNLTLKSNDSIDTVLPSVELAMHTGDICSIQNINHLSHIHMAA
                     LTLLAKSESLMDPVTGQVFHPHPGFGLQGVDEHGVTRTLVR"
     gene            complement(794..1015)
                     /locus_tag="PLD53_08885"
     CDS             complement(794..1015)
                     /locus_tag="PLD53_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HRC61069.1"
                     /translation="MAKPFPLNPKHPERICWGCDKYCSVDSLNCGNGSGRTQHPAELI
                     GEDWYKWGDWGIADPAAKEPEKPADDSCQ"
     gene            complement(1049..1489)
                     /locus_tag="PLD53_08890"
     CDS             complement(1049..1489)
                     /locus_tag="PLD53_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRC61070.1"
                     /translation="MIMSQSAQSDIYRPIIEQIADAIIYSDSEGIIRVWNAAAMEIFG
                     YTKDEAIGQSLDLIIPERLRAAHWVGFNRAMERGMTQHAGRATVTRSLTRDGGSVFVE
                     MSFAVVTDGDGKAMGSVAMARDVTKRHRAEQLLRGRTVTPTHEQ"
     gene            complement(1486..1698)
                     /locus_tag="PLD53_08895"
     CDS             complement(1486..1698)
                     /locus_tag="PLD53_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61071.1"
                     /translation="MMIEVEPNGIYEIELCSGECRRWLYCGSDSQSQIWWRDLETGRE
                     FNESSVMYTWTIIRKIDPSSNLQKAK"
     gene            complement(1823..3022)
                     /locus_tag="PLD53_08900"
     CDS             complement(1823..3022)
                     /locus_tag="PLD53_08900"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRC61072.1"
                     /translation="MNELPANRPARATGTTAPAGFALWNLGFRPFYLLASTFSAFSVL
                     LWAAQFSGNLTATYLSEPLWHGHEMLFGYTTAIIAGFLLTAVRTWTNQPTPSGIPLMA
                     LAALWVAGRVLVLTPLALTAAMVNAAFPLAVAVSIGIPLWKARNTRNYFFVGLLVLMS
                     VLTIAVHMALQGHFELSPYRGLQLALDLVLFIMVVMGGRVIPMFTNNGILGTNATRHP
                     LVEKVVLGSVILLFASDLLNLPQPIIALLALVSALANGWRLFLWKPWRTLATPLVWIL
                     HAAYAWIVVYLILRGMTAMGFLAGSYAAHALTVGTIGGLTLGMMTRTARGHTGRPLVA
                     DGFEITAFLLIQAAAIIRVFGGIASPELYMASIELSGLIWAGAFGLYAVRYWPVLTRP
                     RLDGKPG"
     gene            complement(3070..4476)
                     /gene="ccoG"
                     /locus_tag="PLD53_08905"
     CDS             complement(3070..4476)
                     /gene="ccoG"
                     /locus_tag="PLD53_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRC61073.1"
                     /translation="MLHQPTPRKAIPIKPVFDPEAKVYPRTIKGVFTRWRWIFVWLTQ
                     IVFYGLCWLPWHGRQAILLDIDQRKFYFFDLVLWPQDTIYLVLLMVLGALALFLFTAI
                     AGRLWCGYTCPQTVYTEIFLWFEKVIEGERPQRIKLDAAPWTPQKLAIKAAKHATWMI
                     LSLWTGFTFVGYFTPIHDLARDLFSFSTGGWSSFWVGFYALATYGNAGFLREQMCRQI
                     CPYARFQFVMFDPDTLIIAYDAERGESRGARAKTADPRAQGLGDCVDCSICVQVCPTG
                     IDIREGLQNECIGCAACIDACDQVMDKMAYPRGLIRYSTENAVSKRYGPMDIVRRAFR
                     PRVIIYGAVLFLLAGATAWSLATRIPLKVNVLKDHGTLSREADDGSIENIYELKIMNT
                     GDQTRTFNLSVSGIESIRLENNRPITVRGGEIGSQTIVLSADPGVAKSGASPIRFNVE
                     DVDDATVAVTEKSKFWMP"
     gene            4607..5041
                     /locus_tag="PLD53_08910"
     CDS             4607..5041
                     /locus_tag="PLD53_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRC61074.1"
                     /translation="MRISTFSDYSLRVLMYLGVQTNRLATISEIAAAHEISENHLMKV
                     VHQLGRSGYIETVRGKGGGMRLARTPKEIVLGDVIRQTEGGNPMAECFTDNSSCRILP
                     VCRLRGILGDALSAMFLVFDSYTLADLLEKPQDHLIAIRRKE"
BASE COUNT         1206 a         1396 c         1381 g         1124 t
ORIGIN      
        1 cttattctgg cgtgattatc gctttgagca ccctaacgta gggagacctt caatttccac
       61 tctttctgtt gacgtgtctt ccgttttctt gtgatgtgac agtacggttt ggccaatttg
      121 ataatttcag tcctcgacga tcaagcatgg tggaatgacg ccactcctgc agccttatag
      181 atcattccaa cccagcacgt cggggattaa aagcctgaga aactacgggg gcaccatggt
      241 gaatttaaca ctgaagagca acgactccat tgacaccgtt ctgccatccg ttgagctggc
      301 catgcacacc ggggatatct gcagcattca aaacatcaat caccttagcc acatccatat
      361 ggctgcgctg acattgctgg cgaagtccga aagtctgatg gatccggtga ccggacaggt
      421 ctttcacccg catccggggt tcggtttgca aggtgttgat gaacacggtg tgacacgaac
      481 actggtgagg tgagcttaac gtgcgaaaca ccgttcagct gcgtaacgca ggcattacag
      541 ggtcaatttc cagactttga gcttgacgtg gtccggcata gagcgccgcg caggctattg
      601 gaatacgttc acgcgtcaat gagatttgtg gggtaagcgt tcggattttc gtgctcatga
      661 tgctcgatgc aatgagcaaa atgcgtggat cggatgaaat gcaacagcct gcgaacgggc
      721 aaagatgtgc tgtagaaatc aaatgtcgct tatgtgctcc agcagacaga cggcagaccc
      781 agcaatctcg gaactattga caggagtcgt ccgcaggctt ttccggctcc ttcgccgcag
      841 gatctgctat gccccaatct ccccacttat accagtcttc accaatcaat tcagccgggt
      901 gctgagtacg gccagaacca ttcccacaat tgagcgaatc taccgaacaa tatttgtcac
      961 acccccagca aattcgctcg gggtgttttg gattgagggg gaatggtttt gccatcgttt
     1021 tgccggtact tctgtgattt gggcgcaatt actgctcatg ggtgggcgtg acggtacgcc
     1081 ctcgcaaaag ttgttcagcc cgatggcgtt tggttacatc gcgagccatc gccactgatc
     1141 ccatggcctt tccgtcccca tcagtaacca cagcgaagct catctcgaca aagacggatc
     1201 ctccgtcgcg cgtcagtgag cgggtgactg tcgcccgccc tgcatgctgt gtcataccgc
     1261 gctccatcgc gcggttgaaa cccacccagt gagcggcacg cagtcgctca ggaataatca
     1321 gatccagact ttgtcctatt gcctcgtctt ttgtatagcc aaaaatttcc atcgccgccg
     1381 cattccatac gcgaatgatt ccttccgaat cgctataaat gatggcgtcg gcaatctgct
     1441 cgatgatcgg acggtagatg tcggattgcg cgctttggct cataatcatt ttgctttctg
     1501 gaggttgctc gacggatcaa tttttcgaat gatcgtccag gtgtacatca cactggattc
     1561 gttgaattcg cgtcctgttt ccaaatcacg ccaccaaatc tgtgactgcg agtcagatcc
     1621 gcagtagagc caacgacggc actctccgct gcacaactcg atttcgtaga ttccatttgg
     1681 ttccacctcg atcatcatgg agctcttcta tttctctgta attgacacaa gacaaatcca
     1741 tcagaccatg aacatgacgt acgcaaatcc ttgcggtgag ctacgatcag tgtttgaaaa
     1801 gcatgtggac ggaaacgcct gcttatccag gctttccgtc cagtcggggg cgggtcagaa
     1861 cgggccagta acgcaccgca taaagaccga acgcgccggc ccaaatcaac ccagacaact
     1921 caatactcgc catgtacagt tctggcgatg cgatgccgcc gaagacgcga atgatcgccg
     1981 ccgcctgtat aagcagaaac gctgtgatct caaagccgtc ggcaaccaga ggcctcccgg
     2041 tatggccacg ggccgtccgg gtcatcatgc ccaaggtcaa gccgccaatg gtgcctaccg
     2101 tgagtgcatg ggcggcatag gaccccgcca ggaaccccat ggcagtcata ccgcgcaaga
     2161 tgagatagac gacgatccag gcataggcgg cgtggagtat ccacaccagt ggcgtagcca
     2221 gggtgcgcca cggcttccag agaaacaatc gccagccatt tgccagggca ctgaccaggg
     2281 caagcagggc gatgatgggc tgcggcaagt tcagtagatc ggatgcgaac aacagtatca
     2341 ccgagcccag caccaccttt tccaccagcg gatgacgtgt ggcgtttgtg ccaaggatgc
     2401 cgttattggt gaacatcggg atcacgcggc cccccatgac caccatgatg aagaggacca
     2461 gatccaaggc aagttgcaaa ccccgatacg gcgatagctc gaaatgtccc tgtaaggcca
     2521 tgtgtacggc aatagtaagc acgctcatca ataccagcag accgacaaag aaatagttgc
     2581 gagtgtttcg cgctttccac aacggaatgc cgatggacac cgcaacagcc agaggaaatg
     2641 cggcgttgac catggccgcc gttagggcca acggcgtcaa caccaagact cgaccggcta
     2701 cccagagcgc tgccaaggcc attaatggga tgccgcttgg cgtgggttgg tttgtccacg
     2761 tccgtacggc cgttagcaag aaaccggcaa tgatcgccgt tgtgtatccg aacagcattt
     2821 catggccatg ccatagcggt tccgacaaat aggtagcggt cagatttcct gaaaattgcg
     2881 ctgcccagag caacacgctg aaggcactga aggtactcgc gagtaagtaa aatgggcgaa
     2941 accccagatt ccaaagcgcg aatccagcag gagccgtagt tcctgttgct cgtgctggtc
     3001 tattggccgg taattcgttc attctattat ctgatcttct cgtttggctt tcgttgcaga
     3061 gtcctgccgt caaggcatcc agaatttcga cttctcagtc accgcaacgg tggcgtcgtc
     3121 aacatcctcg acattgaacc tgatcgggct tgcgcccgac ttggccacgc ccggatctgc
     3181 actaagcacg atggtctggc tgccaatttc gccgcccctg acggtgattg gccggttgtt
     3241 ctcgaggcgg atgctctcga tgccgctgac gctgaggttg aatgtccggg tctggtcacc
     3301 cgtattcatg atcttcagtt cgtagatgtt ttcgatgctt ccgtcgtccg cctcacgcga
     3361 cagcgtgccg tggtccttca acacattaac tttcagaggg atccttgtcg ccagcgacca
     3421 ggccgtggca ccagcgagca gaaagagcac cgcgccgtaa atgatcactc gtggcctgaa
     3481 agcccgacgt acgatatcca ttgggccgta gcgcttgctg accgcattct ccgtcgagta
     3541 gcggatcaaa ccgcgtggat acgccatctt gtccattacc tgatcgcatg catcgatgca
     3601 ggcggcgcag ccgatgcact cattctgcaa gccctcgcga atgtcgatgc cggtcggaca
     3661 cacttggacg caaatgctgc aatcgacaca gtcgcccaga ccttgcgcgc ggggatcagc
     3721 cgtcttagcc cgcgccccgc gcgattcgcc gcgctcggca tcgtaggcaa tgatgagcgt
     3781 atcggggtca aacatgacga actgaaagcg agcgtagggg cagatctgcc gacacatctg
     3841 ttcacgcagg aatccggcat tgccgtaggt ggccaaggcg taaaaaccga cccagaagga
     3901 cgaccagcct cccgttgaga atgagaaaag atcgcgcgcc agatcgtgaa tcggcgtgaa
     3961 atagccgaca aaggtaaagc cagtccacaa cgaaagaatc atccaggtag catgcttggc
     4021 tgccttaatc gccaattttt gcggcgtcca gggcgccgcg tcgagcttga tacgctgcgg
     4081 tcgctcgcct tcgatgacct tttcgaacca cagaaagatt tcggtataaa cagtctgcgg
     4141 gcaggtatag ccgcaccaca ggcgaccggc gatggcggtg aataggaaca gcgcgagcgc
     4201 accgaggacc atcagcagga cgagatatat cgtgtcctgt ggccacagca ccaagtcgaa
     4261 gaagtaaaac ttgcgctgat cgatatcgag caggatggcc tgcctgccgt gccagggcag
     4321 ccagcacagg ccatagaaaa caatctgggt cagccagacg aaaatccagc gccagcgggt
     4381 gaaaacgcct ttgatcgtac gcggatatac cttggcctcg ggatcgaaga ccggcttgat
     4441 aggtatggct ttccggggcg tgggctgatg gagcatcgct ctcgtgaggt ccgattctgg
     4501 tggattgcgt ttgagtattg caataagatg caaatagatt gcatctacat gctatcaatc
     4561 ccttctcaaa taagcaataa tatttataca tcttagatgg gcgtccatgc gaataagcac
     4621 attttccgac tatagcctgc gcgtgctgat gtatttgggc gttcagacta accgcctggc
     4681 gaccatttca gagattgctg cggcgcatga gatttccgaa aatcacctga tgaaggtggt
     4741 acatcaactc gggcgtagcg gatacatcga gacagttcgc ggcaaaggcg ggggcatgcg
     4801 cttggcgcga acaccgaaag aaatcgtact gggcgacgtg attcggcaaa ccgaaggcgg
     4861 caacccgatg gccgagtgtt ttactgataa ttcttcgtgc agaattctgc ccgtgtgccg
     4921 tttgcgtggc attcttggtg acgcgttaag tgcgatgttt ctggtgttcg atagctacac
     4981 gctggccgat cttctggaaa aaccgcagga ccatttgata gccattcgcc ggaaagaatg
     5041 agtgactcaa gacgatctga cgaattagat ccaagctggc aagctgtgga caaatgtcgg
     5101 cacgatg
//
LOCUS       DAORPM010000180         5073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4711_length_5073_cov_11.024910, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000180 DAORPM010000000
VERSION     DAORPM010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4711_length_5073_cov_11.024910"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1557
                     /locus_tag="PLD53_08915"
     CDS             175..1557
                     /locus_tag="PLD53_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HRC61075.1"
                     /translation="MIKVFSHWLHLKTVLRILIDFIFPVICVLLAVVLVGHRGQESIE
                     SVIIYAVVFALAMIVFNASLGLYQRSHGRTIMQTRARAVLSLYLSVPLAYGVFALLSI
                     ADADHELLLVSGLAALFGTLAHRVYTMHRRTGTMMEHRVLIFGAGPVAESVSKVLKRS
                     DPDIQIVGYYPSPTDTEICIPGQLVLPRAQSLSDTAYALNVHEIIIAVRERRGGILPL
                     RELLDCKLSGVNVLDLASYFERALGQIRLDSLRIGWLIFGEGFRQGWQRTFVKRLFDI
                     VAASILLLLALPMMLVTALLIVLEDGFPVFYRQERVGLNGRLFNVIKFRSMRKDAESD
                     GKPRWATANDDRTTRIGRIIRKLRIDELPQLYSVLTGDMSLVGPRPERPYFVDQLTRE
                     IPFYAVRHSVKPGVTGWAQVSYPYGASVDDSIQKLQYDLYYVKNHTLFLDIVILCETV
                     VVVLTGKGAQ"
     gene            1614..3737
                     /gene="prsK"
                     /locus_tag="PLD53_08920"
     CDS             1614..3737
                     /gene="prsK"
                     /locus_tag="PLD53_08920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HRC61076.1"
                     /translation="MDASGEAITVWSYGLAGFVYAAFAIYLASGWRAGLRNRALFSAA
                     VLCALWGMAGLAYALTGQTVFLAGGMLADLFRYGGWYFFLLVLLVPDSSREDRWPNRI
                     RWLVGVALALLLIGLAAQVMVLLGMNDFFPPMRLVIFDSLAMAVFALVLIEQLFRNVS
                     PDWRWGIKPLCIGLGGAALFDLYLFSDALLFSRVDADAFNIRGFVHALVVPLVVVSAV
                     RSKDWKTRIVLSQRAALQSATLLAVGVYLLFVSLAGYYVRYFGGDWGRAFQLALLFAA
                     LLLLGMLSLSGSMRARFRVMVGKHFFSYRYDYREEWLRFTNTLSSQDGFAEMGRHVVK
                     GLADMVESPAGALWLKDPTERFFAQAACWHMPVSSATEDADSKLCQFLLDSGWVINLE
                     EYRSLPRRYDHLDLPVWLVEVPNAWLVVPLATGSEMTGFVVLSTARTKIDVNWEVNDL
                     LKTAGRQAGAFLGQMQATEALLESRKFDAFNRMSAFVVHDLKNIIAQLSLMLKNAERH
                     RDNPEFQQDMLATVEHSVERMRQLMMQLREGATPIDSPRGIDLGGVIRRIQAAKAGQG
                     RHLEIDLIDKVVAKGHEDRVERVIGHIVQNALDATENGGRVWVRLERQGDHAMVEVGD
                     TGSGMSPEFLRERLFKPFQTTKPAGMGIGAYESFQYVHELGGKISVDSAINVGTQVSL
                     LLPLFDAGVEMGADLDSERMHEESK"
     gene            3734..>5073
                     /gene="prsR"
                     /locus_tag="PLD53_08925"
     CDS             3734..>5073
                     /gene="prsR"
                     /locus_tag="PLD53_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HRC61077.1"
                     /translation="MSSEKLNHLLIVEDDPSLQKQIRWAFDQYETLTADDRESALVQV
                     QRFMPPVVTMDLGLPPDADSVSEGFKLLEQILSIAPETKVIVLTGQNDRANALRAIGL
                     GAYDFFAKPFEIDLLGLTIERAYRLYELQQENNRLQLMQQPDVLSGLISRDPEVLKVC
                     RTVEKVAISDATVLVLGESGTGKELIARGVHAASARRGERFVAINCAAIPENLLESEL
                     FGYEKGAFTGAAKTTPGKIETANRGTLMLDEIGDLPHSLQAKLLRFLQERVVERIGGR
                     QEIPVDVRIVCATHQDLKALIKEGRFREDLYYRLAEIIVLIPPLRARQGDAALLAHAF
                     VRRFAEEQNRGAMTLREDALRIIETYSWPGNVRELENCIKRAVIMADGNQITVDDIGL
                     EEVQNESEARLDIRQAREDAEKRVVITALGRADGNVHKAAEMLGVSRPTLYDLM"
BASE COUNT         1044 a         1189 c         1509 g         1331 t
ORIGIN      
        1 aacgaatcta cagggatata ttcttcttgt gcattttgtc acggttgata aaacagcgtg
       61 ctgttaatct ggttcgcaag agattcgaaa caggcgtcac tttaggtacg gcgtatccgt
      121 ttggaatctc gatgacagaa gaattgttta gatgcatcag actaggggca aatcgtgatt
      181 aaggtattca gccattggct acatttgaag acagtcttgc ggattctgat cgacttcatt
      241 tttcctgtta tctgcgtttt gctggcggta gttttggttg gacaccgagg ccaggagagt
      301 attgaaagcg tcatcattta cgctgtcgtc tttgcattgg cgatgattgt attcaatgcc
      361 tcgcttgggc tttatcagcg ctcgcacgga cgaaccataa tgcagacacg cgctcgcgcg
      421 gttctctctc tctatctatc agtccctctt gcctacggtg tgttcgcctt gctttcgatc
      481 gccgatgctg atcacgaact cctgcttgtc tcgggtttgg ctgccttgtt cgggacgctt
      541 gcccatcgcg tatatacaat gcatagaaga acaggaacga tgatggagca tcgtgtgctg
      601 atctttggtg ccggcccagt ggctgaatcc gtaagcaagg tactgaaacg gtccgatccg
      661 gatatacaga tcgttggtta ctatccgagt ccaaccgaca cggaaatatg cattccgggt
      721 cagcttgtgt tgccacgggc ccaatcactg tcggacacgg cgtacgcctt gaatgttcac
      781 gagatcatca tcgctgtacg cgagagacgt ggtggtattc tgcccttgcg agaactcctc
      841 gactgcaagt tgtcaggtgt taatgtgctt gatctggcca gctattttga gcgggcactc
      901 ggccagattc gactcgattc cctgcgaatt ggctggctca tatttggcga aggttttcgg
      961 caggggtggc agcgcacgtt tgtcaagcgt ctgtttgata tcgtagctgc ttcgatcctg
     1021 ctcctgctcg cattgccaat gatgctcgtc acagcactgt tgatcgtgct ggaggacggt
     1081 ttcccggtat tttatcgaca ggaacgcgtg ggtttgaatg gccgcctgtt taatgtcatc
     1141 aagttccgca gcatgcgaaa ggatgctgaa agtgacggta agccacgctg ggcgacggca
     1201 aatgatgatc ggacgacccg aattggacgt attattcgca agttgcgtat tgatgagttg
     1261 ccgcagcttt acagcgtgct gaccggtgat atgagtctgg tcggtccgcg tccggagcgt
     1321 ccgtactttg tcgatcagct gactcgtgaa attccgttct acgcggtccg tcacagtgtc
     1381 aagcctggag taaccggctg ggcgcaagta agttacccgt acggagcctc tgttgatgat
     1441 tctatccaga agctccagta tgatctttac tatgtaaaga accacactct ttttctagat
     1501 atcgtgatct tgtgcgaaac ggtagttgta gtgcttaccg gtaagggcgc tcagtagcgt
     1561 cactgtccgc gggtgagtcg tattccggaa cgcaatctga ggaggttcgt aggttggatg
     1621 ccagtggaga agcaattaca gtctggagct acggacttgc aggcttcgtc tacgccgcat
     1681 ttgccattta tctggcttca ggttggcgtg ccggcttgcg caaccgcgcc ttgtttagtg
     1741 ctgcggtgct ttgcgcgctc tgggggatgg ctggtcttgc ctatgcttta acggggcaaa
     1801 cggtattctt ggccggcggc atgcttgccg acctcttccg ctatggcgga tggtatttct
     1861 tcctgctggt gctactcgtt ccggattctt ctcgagaaga ccgctggccg aaccgcattc
     1921 gctggctggt tggagtggcg cttgcgttgc ttctgattgg tcttgcagca caagtcatgg
     1981 tcctgctggg catgaatgac ttcttcccgc ccatgagact agtcatattc gactcgctgg
     2041 cgatggcagt gtttgcactg gtactcattg aacagctgtt tcgcaacgtg tcacctgatt
     2101 ggcgttgggg catcaaacca ttgtgtattg gccttggcgg tgctgccctg tttgatttgt
     2161 acttgttctc ggacgcattg ttgttcagta gggtcgatgc ggatgcattc aatatccgcg
     2221 gcttcgtgca cgccttggtg gtgccgctgg tagtcgtatc ggctgttcgc agcaaagact
     2281 ggaaaaccag aatcgtatta tcccaacgcg ccgcgctgca atcagcgact ttgcttgcgg
     2341 ttggtgtgta ccttctgttt gtatccttag ccggttatta cgtgcgctat tttggcggcg
     2401 attgggggcg tgccttccag ttagccctgt tgtttgcggc gctgctgctg ctgggcatgt
     2461 tgagcttgtc ggggtcaatg cgggccaggt tccgcgttat ggtcggcaag cattttttca
     2521 gttatcgcta cgattatagg gaagaatggc ttcggtttac caataccctg tcatctcagg
     2581 acggttttgc agaaatgggg aggcatgtgg tcaaaggctt ggccgatatg gtcgagagcc
     2641 cggccggtgc gctctggttg aaagatccga cagagcggtt ttttgcgcag gcggcttgct
     2701 ggcacatgcc tgtgtcgtcg gcgactgaag atgccgatag caagttatgt cagtttttgc
     2761 ttgacagtgg ctgggtaatc aatcttgagg agtaccgttc tctgccacgt cgctacgacc
     2821 atcttgatct tcctgtctgg ctggtcgaag tgccaaatgc ctggttggta gttcctctgg
     2881 cgacgggaag cgaaatgacc ggcttcgtcg ttctatcaac ggcacgtacg aagatcgacg
     2941 tcaactggga ggtgaacgat cttttgaaga cggctggccg gcaagccggg gcatttctgg
     3001 ggcagatgca ggcgactgag gcattgttgg agtcgcgtaa atttgacgcc ttcaaccgaa
     3061 tgtccgcttt tgttgtacac gacttgaaga acattattgc tcaattgtcg cttatgctga
     3121 agaatgctga acgccatcgg gataatcctg aattccagca ggatatgctt gcgactgtag
     3181 agcattcggt cgaacgtatg cggcaattga tgatgcagtt acgcgaaggc gcgacaccga
     3241 ttgacagtcc acgcggtatt gaccttggtg gtgtcatccg acgtatccag gcggccaagg
     3301 cagggcaggg tcgccatctg gagatcgatc tgattgataa ggtagttgcc aaggggcacg
     3361 aagatagagt cgagcgagtg atcggacaca ttgtgcagaa tgcgctggac gccaccgaaa
     3421 atggtggacg ggtttgggtc agactggagc gccagggaga tcatgccatg gtcgaggtcg
     3481 gtgatacagg cagcggcatg tcgccggaat tcttgcgcga acgcttgttc aagcccttcc
     3541 aaacgaccaa gccggcgggt atgggcattg gcgcatatga gagcttccag tacgtgcacg
     3601 aattgggtgg caagatttct gtggatagtg caatcaacgt cgggactcag gtgagtttgc
     3661 tattgccctt gtttgatgct ggtgtcgaaa tgggagctga tttggattca gaacggatgc
     3721 atgaggaatc caaatgagtt cggagaaatt gaatcacctg ttaatcgtgg aggatgaccc
     3781 ttccctacaa aagcagattc gctgggcttt cgatcaatat gagacactca cggcagatga
     3841 tcgcgagagt gcgcttgtcc aggtgcagcg ttttatgccg ccggtcgtca caatggatct
     3901 cggcctgcca cccgatgccg attctgtttc cgagggcttc aagttactcg agcaaatttt
     3961 gtcgatcgct cccgagacca aggttattgt cctgaccggc cagaatgacc gggccaacgc
     4021 gctgcgtgct atcggcttgg gtgcctatga tttttttgca aagccgtttg agatcgatct
     4081 gttgggattg acgatagaac gcgcctaccg tctctatgaa ctccagcaag agaacaaccg
     4141 cttgcagctt atgcagcagc cggatgtctt gtcgggactg atttcccggg atcccgaggt
     4201 cttgaaggta tgtcgtacgg ttgaaaaggt tgcgattagc gacgcaaccg tgctggtgtt
     4261 gggcgagagc ggaacgggca aggaattgat tgcacgcggg gttcatgctg catccgcgag
     4321 gcggggagag cgctttgttg ccattaactg tgccgcgatc ccggagaatc tgctggaaag
     4381 cgagctgttc ggctacgaga aaggtgcttt tactggtgcg gccaaaacca caccaggcaa
     4441 gattgagacg gcgaaccgcg gcaccttgat gctcgatgaa ataggcgatt tgccacattc
     4501 gctgcaggcg aaactgttac gattcctgca ggaacgggtt gttgaacgca ttggtgggcg
     4561 acaggagatt ccggttgacg tccgtattgt gtgcgcaacg caccaggacc tgaaagcatt
     4621 gataaaggaa ggccggtttc gtgaagacct ctattaccgt ctggcagaaa tcatcgtact
     4681 cattccacct ttgcgcgcac gccagggcga tgcggcgttg ctggcacacg cttttgtacg
     4741 ccggttcgct gaagagcaga atcgcggtgc catgaccttg cgtgaggacg ctttgcgaat
     4801 cattgagacg tattcttggc caggcaatgt tcgagagctt gaaaactgta ttaagcgtgc
     4861 ggtcatcatg gcggatggaa atcagattac tgtggacgac attggtctcg aggaggtgca
     4921 gaatgagagc gaggcgaggc tggatatccg tcaagcacgc gaggatgctg agaaacgcgt
     4981 cgtcataact gccttggggc gtgctgatgg gaatgtgcat aaagcagccg aaatgctggg
     5041 tgtcagcagg ccgacgcttt acgatttgat gca
//
LOCUS       DAORPM010000181         5042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4755_length_5042_cov_8.234008, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000181 DAORPM010000000
VERSION     DAORPM010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4755_length_5042_cov_8.234008"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /gene="phnH"
                     /locus_tag="PLD53_08930"
     CDS             <1..442
                     /gene="phnH"
                     /locus_tag="PLD53_08930"
                     /inference="COORDINATES: protein motif:HMM:NF017645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HRC61078.1"
                     /translation="RRVVLATLMDGESTLADPHGMIPDEDWPLLQARRDTAESARYVV
                     VDGSRDATLNPCLGRLESPEFGATLLIAVEEIGTGSLSIALAGPGIEERLELCLSGLH
                     PDWLARREDWVGAFPLGVDILFCDAQRIVALPRTTHAAITAGVV"
     gene            444..1556
                     /locus_tag="PLD53_08935"
     CDS             444..1556
                     /locus_tag="PLD53_08935"
                     /inference="COORDINATES: protein motif:HMM:NF017659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HRC61079.1"
                     /translation="MSYVAIKGGGHAIAGAAAVSEYLRTEQGLDNSNAEPLSIDTLLH
                     QLRHLVDRVVSEGGVYHPQFAALAIKQSLGDTLEAAFALRAWRSTRPRLSATPHLDTS
                     RMRLIRRISSAFKEIPGGQMLGPTPDYALRLLRLELMDESPAAFRSVARDWLDGCPEA
                     TLPDSFPKVLDALRAEGLLPPVGTDRNQFAFDITREPLVFPVPRSAALATMARAETGS
                     LLAMAYSNMRGYGDVHPTIAELRVGYLPVELPHPVTGELTEAGEVLVTECEVVAMYDA
                     AANGGKPTFTIGYGACFGHNEVKAISMAVLDRALQKGMKDGAANPSEDPEFVLLHIDG
                     VDSMGFASHYKMPHYVTFQSDLDRLRTTQQKAGEPA"
     gene            1553..2452
                     /locus_tag="PLD53_08940"
     CDS             1553..2452
                     /locus_tag="PLD53_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HRC61080.1"
                     /translation="MIVTDNNSTANAYNFGFLDEYAKKEIRRAILKAIAIPGYQVPYA
                     SREMPMGRGFGTGGLQLTLSLIGPDEVVKVIDQGSDDSVNAVNLRQFIEMTCPGISTT
                     TKTDESSLIQSRHRIPERPLTSQQIIVLQVPYPDPLVVVEPSEDKRKTMHGEADYSRL
                     LVKLYEDIVAFDEITISHRYPTRINGHYVIDPSPIPRWDVPKLNDSAALILFGAGREK
                     KIYAVPPHTRAEPLVFSDVPFRVEDFYDAAGRRRACTRCGCADSFLDEFVNESGSKSY
                     QCSDSDWCDEQLLRVDEVEGEVA"
     gene            2449..3279
                     /locus_tag="PLD53_08945"
     CDS             2449..3279
                     /locus_tag="PLD53_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC61081.1"
                     /translation="MNKILEVRNLSKIYGPGCPLCFESTGPEADTNLCPNCASVIAIH
                     DVSFDLFEGEILGVMGESGSGKSTVVKSLYFDQAPSSGEAIFYDRDKQHDLFSLNAAQ
                     QRWLRNHRFGMVYQNPNLGLNFNISAGGNIAERLLMSDLMDYGAIRARAKALLARTEV
                     LLSRVDDLPKNFSGGMQQRVQIAKALATQPPLLFLDEVTSGLDLSVQARILDLILEIQ
                     QELKTAMVVVTHDLGVIRLLTGRAIVMKYGRVIEAGLTDQILEDPQHAYTQRLVASAL
                     "
     gene            3290..4012
                     /gene="phnL"
                     /locus_tag="PLD53_08950"
     CDS             3290..4012
                     /gene="phnL"
                     /locus_tag="PLD53_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HRC61082.1"
                     /translation="MYPLILQVDGFGKHFQLHEQGKLIPSSYDVSFSVRSGQLTALIG
                     PTGAGKSSVLKGIYRSYLPSAGRILYRNAMGHELDLALASEHQILELRRREIAFVTQF
                     LHCLPRQSTLDVVAQPLFSLGVSRDESRRRAAELLFAMNLPERLWSVSPATFSGGERQ
                     RVNLARGFIAKPRLLLLDEPTASLDPKTAECVISLIDGLKFEGTAMLAIFHNPETTRR
                     LADQVVELAMPPAVEDILKEIA"
     gene            4009..>5042
                     /locus_tag="PLD53_08955"
     CDS             4009..>5042
                     /locus_tag="PLD53_08955"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HRC61083.1"
                     /translation="MTRLYLTGARVVLRDALLDDASVLIEDGIITAINPESGNGAENV
                     DLRGKTLIPGMIDLHCDALEKEVEPRPNVYFPLDFACAQADKRNAAAGVTTVYHALSF
                     ANDELGMRNNGFAASVARAVHAWNPHGLVDNRVHCRYEVTDPTAPTVLLELMASNEMH
                     LLSVMDHSPGQGQFKDIAAFRDYLSRTYKKSEAELDTLVDNKLAQADGAMARIRLLIE
                     AARNKGIPVASHDDDTPDKVETLHALGVGISEFPINLESARAARDRGMSTVFGAPNIL
                     RGKSQSGAVRALDAVQAGLAECLCADYHPAALIVAVFRLPELAGIGLDDAVRLVSYNP
                     AQAAGLIDRG"
BASE COUNT         1010 a         1519 c         1461 g         1052 t
ORIGIN      
        1 ccggcgcgtt gtgctggcca ccctgatgga cggagaatca acgctggccg acccacatgg
       61 aatgattccc gacgaggatt ggccattgct ccaggcacgg cgagatactg cagagagcgc
      121 acgttacgtc gtggtcgacg gcagtcggga tgcgaccctt aacccgtgcc tcggccgtct
      181 ggaaagtccg gagttcggtg ccaccctgct gattgcagtc gaggaaatcg gaaccggctc
      241 cctgtcaata gcacttgccg gacccggtat cgaggagcgc cttgagctgt gcctgtcagg
      301 cctccatccc gactggctgg ctcgccggga ggactgggtc ggtgcctttc ccctgggcgt
      361 ggacattctg ttttgcgatg ctcaacgcat cgtcgcgctg ccgcgtacga cgcacgcggc
      421 aatcacggct ggggttgtct gagatgagtt atgttgccat caagggcggc ggccacgcga
      481 ttgcgggtgc ggcggccgtc agcgaatatc tgcgtaccga acagggcctg gacaacagta
      541 acgccgagcc gctgtcgatt gacactctgc tgcatcagct tcgccatctg gtcgatcgtg
      601 tcgtatccga aggtggtgtg tatcatccgc agttcgctgc cttggccatc aagcaaagcc
      661 tgggcgacac attggaagcc gctttcgcgc tgcgcgcttg gcgttcgacg cgaccccgcc
      721 tgtcagcgac gcctcacctt gatacctcac gcatgcgact gattcgccgt atctcgtccg
      781 ccttcaagga gatacctggc ggacagatgc tcgggccgac cccggactat gccttgagac
      841 tgctcaggct ggagctgatg gacgagtctc cagcagcctt ccggtcggtt gcccgcgatt
      901 ggctggacgg ttgtccggaa gcaacgttgc cggacagctt ccccaaggtg cttgacgcct
      961 tgcgcgccga ggggctgtta ccgcctgtcg gcactgaccg aaaccagttc gccttcgata
     1021 tcacacgcga gcctcttgtt tttcctgtac cccgttccgc tgccctggcg accatggcgc
     1081 gggcggaaac cgggtcgcta ttggccatgg cctattccaa catgcggggt tatggcgatg
     1141 tacaccctac gatcgctgag cttcgtgttg gctacctgcc ggttgaactg ccgcatccgg
     1201 tcaccggcga attgaccgag gccggtgaag tactggtcac cgaatgcgag gttgtggcga
     1261 tgtacgacgc agctgcaaac ggtggaaaac cgacttttac catcggttac ggcgcctgtt
     1321 tcggtcataa cgaggtcaag gccatttcga tggctgttct ggaccgtgcc ctgcaaaagg
     1381 gcatgaagga tggtgcggcc aacccttcgg aagaccctga atttgtcctt ctgcatattg
     1441 acggcgtcga ttccatgggc ttcgcatcgc attacaaaat gccgcactac gtcacttttc
     1501 agtccgacct ggatcgtctg cgcacgacgc agcaaaaggc aggagaaccc gcatgattgt
     1561 cacggacaac aactcaactg ccaacgccta caacttcggc tttctggacg aatatgccaa
     1621 gaaggaaatt cgtcgcgcca ttctcaaggc gatcgccatt ccaggctatc aagttcccta
     1681 tgcttcgcgt gaaatgccca tggggcgcgg ttttggcacc ggtgggctgc aacttaccct
     1741 gtcgctgatc ggcccggacg aggtggtcaa ggttatcgat cagggatcag acgattcagt
     1801 caacgcagtc aacctgcgcc agttcatcga gatgacctgc cccggcattt cgacgaccac
     1861 gaaaacggac gagagctcgc tgatccagtc tcgccatcgt atcccggaac ggccactcac
     1921 gtcgcagcag atcatcgttc tgcaagttcc ttatcccgac ccgctggtag tggtcgagcc
     1981 atccgaggac aaacgcaaga cgatgcacgg cgaggctgac tattcgcgac tcctggtcaa
     2041 gctctacgag gacatcgtcg ccttcgacga aatcactatt tcgcaccgct acccgacacg
     2101 catcaatggt cattacgtca tcgacccttc gccgatacca cgatgggacg tgccgaaact
     2161 caacgattcg gcggccttga ttctgttcgg cgccggtcgc gaaaagaaga tctacgccgt
     2221 tccaccgcac acccgggccg aaccgctagt gttctccgac gtccctttcc gtgtcgagga
     2281 tttttacgat gcagccggtc gccgccgcgc ctgcacacgc tgcgggtgcg ccgattcctt
     2341 tctcgatgag ttcgtcaacg aatcgggcag caaatcctac cagtgctcgg acagcgattg
     2401 gtgcgacgag caactgctgc gagtagatga agtcgaggga gaagtggcat gaacaagatt
     2461 ctcgaagtcc gcaacttgag caaaatctac gggcccggtt gtccgttatg cttcgaatcc
     2521 acgggcccgg aagcggatac gaatctctgc cccaattgcg ccagcgtgat cgcaattcat
     2581 gacgtgtctt tcgacctgtt cgaaggcgag attctcggcg tcatgggcga gtcgggcagt
     2641 ggaaaatcca cggtagtcaa gagcctctat ttcgaccagg caccatccag cggtgaagcg
     2701 atcttctacg accgtgacaa gcagcacgac ctcttctcgc tcaatgccgc acagcaacgc
     2761 tggcttcgca accaccgctt cggcatggtc tatcagaatc cgaatctggg cctgaacttc
     2821 aatatcagcg ccggcggcaa tatcgctgaa cggctgctga tgagcgattt gatggactat
     2881 ggcgccattc gggcacgggc caaggcgtta ttggcacgga ccgaggttct gctgtcgcgg
     2941 gtggatgatt tgccgaagaa tttttccggc ggcatgcagc agcgcgtgca gatcgccaag
     3001 gcgctggcta cccagccacc gctgttgttt ctcgacgaag tgacttcagg actggatctt
     3061 tcggtgcagg cccgcattct cgacctgatc ctggagatcc agcaggaact gaagacggca
     3121 atggtcgtcg taacacacga ccttggcgtc atacgcctgc ttaccggacg cgctatcgtc
     3181 atgaaatacg gtcgcgtcat cgaagcaggg ctgaccgatc aaatcctcga agacccccag
     3241 cacgcctata cccagcggct ggtggcttct gccttgtgag gctggcgaaa tgtatccgct
     3301 cattcttcag gtcgatggtt ttggaaagca ttttcaattg cacgagcagg gcaaactgat
     3361 accgtcttcg tacgatgtca gcttttcggt gcgctctggc caactgacgg ccctgatcgg
     3421 acccaccggt gccgggaaat cctcagtgtt gaaggggatc taccgtagct atctgccgag
     3481 cgccggccgc attctctatc gcaatgcgat gggtcatgag cttgatctgg cgctggccag
     3541 cgagcatcag atactggaac tccgacggcg cgagatcgca tttgtcacgc agttcctgca
     3601 ttgcctgcct cgccagtcaa cgctcgatgt tgtggcgcaa ccactgttca gtcttggggt
     3661 ttcgcgcgac gaatcaaggc gacgcgcagc cgagttgctt tttgccatga atcttccaga
     3721 acgcctgtgg agcgtttcgc cggcgacctt ttccggcggc gagcggcaac gggtaaatct
     3781 tgcgcgtggc tttatcgcca aaccgcgtct gttgcttctt gacgagccaa cagccagcct
     3841 cgacccgaaa acagcagaat gcgtgataag cctgattgac ggtttgaagt tcgaaggcac
     3901 tgccatgctc gccatttttc acaaccccga aaccacacgc cgtctggccg accaggtcgt
     3961 tgaactcgcc atgccgccag ccgtcgagga tatcctcaag gagatcgcat gacccggctt
     4021 tatctgacag gagcacgcgt ggtgctgcgt gatgccctgc tcgacgacgc ttcggtgctg
     4081 atcgaagacg gcatcatcac cgcgattaac cccgagtccg gcaacggtgc ggaaaatgtc
     4141 gacctgcgcg gcaagacgct gatccccggc atgatcgacc tgcattgcga cgcgctggag
     4201 aaggaagtcg aaccgcgacc gaatgtctat ttcccgctcg actttgcctg tgcccaggcc
     4261 gacaagcgca atgctgccgc cggcgtgact accgtctacc atgccctgtc cttcgccaac
     4321 gatgaactcg gcatgcgcaa taatggattt gccgcatcgg ttgcccgggc ggtgcacgca
     4381 tggaatccgc atggcctggt cgataaccgg gtgcactgcc gctacgaagt gactgatcct
     4441 acggccccaa ccgttttgct ggagctgatg gcgtcaaacg agatgcatct actgtcggtc
     4501 atggatcatt ctcccggtca gggccagttc aaggacatcg ccgccttccg cgactacctt
     4561 tcgcgcacct acaagaagtc agaagctgag ctcgatacgc tggtcgataa caagctggcg
     4621 caggctgacg gggcgatggc gcgaatccgc ttgctgattg aagccgccag aaacaaggga
     4681 atccccgttg cgagtcatga cgacgatacg ccggacaagg tcgaaaccct gcacgcgctg
     4741 ggcgtcggca tctccgaatt cccgatcaat ctggaatcgg cacgcgcagc tcgcgatcgt
     4801 ggcatgagta cggtattcgg tgcgcccaac atcctgcgtg gcaagagcca gtcgggcgcc
     4861 gtacgagcgc tggatgcggt gcaggccggc ctcgccgagt gcctgtgcgc cgactatcat
     4921 ccggccgcat tgatcgtcgc cgtattccga ttgccggaac tggcgggaat cgggttagac
     4981 gatgcggtgc gactggtcag ctacaatcca gcccaggctg ccggactgat cgatcgtgga
     5041 ga
//
LOCUS       DAORPM010000182         4956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_4904_length_4956_cov_7.741889, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000182 DAORPM010000000
VERSION     DAORPM010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4904_length_4956_cov_7.741889"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1834)
                     /locus_tag="PLD53_08960"
     CDS             complement(<1..1834)
                     /locus_tag="PLD53_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC61084.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALIDNPSAAEAL
                     RSCGAKPESLRKDLTNFINEHTPTVTGEDDIDTQPTLGFQRVIQRAILHVQSSGKKEV
                     NGANVLVAIYGEKDSHAVYFLQKQGITRLDVVNFISHGIAKVPQQKSSPEGEVETEGE
                     KEQSGPLEQYTINLNALALQGKIDPLIGRDREIERVIQTLCRRRKNNPLLVGEAGVGK
                     TAIAEGLARNIVEGDVPDILAKANVYSLDMGSLLAGTKYRGDFEQRLKGVIKQLGDNP
                     NAILFIDEIHTLIGAGSASGGTLDASNLLKPALSSGQLKCIGATTYTEFRGIFEKDSA
                     LSRRFQKIDVNEPSVAETVEILKGLKARFEAHHGIKYSATAISSAAELAARYITDRHL
                     PDKAIDVIDEAGAAQRILPKSKQKKVINKTDIEEIVAKIARVPSQHVTLDDRGALKNL
                     DRDLKAVVFGQDKAIDALARAIKMARSGLGNPGKPIGSFLFSGPTGVGKTEVAKQLAY
                     CLGIELVRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVTKKPYCVLLLDEIEK
                     AHPDIFNILLQVMDHGTLTDNNGRKADFRNVVIIMTTNAGAADLN"
     gene            complement(1834..2142)
                     /gene="clpS"
                     /locus_tag="PLD53_08965"
     CDS             complement(1834..2142)
                     /gene="clpS"
                     /locus_tag="PLD53_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRC61085.1"
                     /translation="MATKSQEGSLLEAQRAKAMPPKMYKVLLLNDDYTPMEFVIVVLQ
                     RFFAMGIEQATRIMLKVHNEGRGICGRFPRDIAATKVEQVGAFARQYQHPLACIMEEN
                     "
     gene            2401..2604
                     /locus_tag="PLD53_08970"
     CDS             2401..2604
                     /locus_tag="PLD53_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRC61086.1"
                     /translation="MAIGTVKWFNDSKGFGFITPDDGSEDLFAHFSAINMNGFKTLKE
                     GEKVSFDVVQGPKGKQASNIQRA"
     gene            complement(2716..3228)
                     /gene="moaC"
                     /locus_tag="PLD53_08975"
     CDS             complement(2716..3228)
                     /gene="moaC"
                     /locus_tag="PLD53_08975"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRC61087.1"
                     /translation="MVTDQTLTHFDAAGQAHMVDVGGKAETVRVARAAGSILMQPATL
                     ALIRAGSASKGDVLGIARIAAIQASKRTSELIPLCHPVALTRVSAEFVIDVAQNAVHC
                     AVTAECLGRTGVEMEALTATAVALLTIYDMCKAVDRGMRIENVRLLEKSGGKSGHWVT
                     SEATNATTSD"
     gene            3291..4739
                     /locus_tag="PLD53_08980"
     CDS             3291..4739
                     /locus_tag="PLD53_08980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRC61088.1"
                     /translation="MSVSHRLLAGFLAFALIAQPLRADDLPELGDVASNELSLATEKK
                     IGQKIMDEIRWREPSYLDDPDVENYLNQLGARLVAVSNDPGMGFQFFAIDDMSINAFA
                     MPGGFIGVHVGLLLSAQTESELAGVLAHEISHVTQRHIARQVYQSKQLSMASMVAMGL
                     ALLAARSSPQGAMAAISTTQAGAISAQLAFSRDFERESDRLGFEILRRAGYDVRGMSA
                     FFGRLQQSTRQYENNATAYLRTHPLSGERMSDMQNRERPVAYRQVADSPEFHLVRARV
                     RALQGRPVDAVKEFEVLLRERKYASEASTRYGLAVALARNRDWAAAEQELAVVRKLKV
                     ASPMVDRQLAEVKIGRGEKEAGLTLYREAMVRYPLNMPLLYGYGSALLAEKQFSAALR
                     FADDQLQSHPQDARFLKLRADSYAGLGRRSQHHLALAELTALKGQTAGAIEQLQLAQQ
                     AGDANFYEMSMIDGRLREMKKRQMEEMKERRN"
     gene            4777..>4956
                     /locus_tag="PLD53_08985"
     CDS             4777..>4956
                     /locus_tag="PLD53_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRC61089.1"
                     /translation="MEIDRNLDVKGLNCPLPILRTKKALAEMESGQVLRVQATDPGSV
                     KDFAAFAKQTGNELVG"
BASE COUNT          912 a         1439 c         1565 g         1040 t
ORIGIN      
        1 tgttcagatc ggcggcgccg gcattggtcg tcatgatgat cacgacattg cggaaatcgg
       61 ccttgcgccc attgttgtcg gtcagcgtgc cgtgatccat gacctgcagg aggatgttga
      121 agatgtccgg gtgcgccttt tcgatttcgt cgagcagcag cacgcagtac ggcttcttgg
      181 tgaccgcctc ggtcagcagt ccaccctggt cgaacccgac ataaccaggc ggcgcgccga
      241 tcaggcggga gacggcatga cgctccatgt attcggacat gtcgaaacgc accagctcta
      301 tgccgaggca gtaggccagt tgcttggcga cctcggtctt gccgaccccg gtcgggccgc
      361 tgaacaggaa ggagccgatc ggcttgcccg gattgccgag tccggagcgg gccatcttga
      421 tggccctggc cagcgcgtcg atcgccttgt cctggccgaa gacgacggcc ttgagatcgc
      481 ggtcgagatt cttcagcgcg ccacggtcgt cgagcgtcac gtgctgcgac gggacgcggg
      541 cgatcttggc gacgatttcc tcgatgtcgg tcttgttgat gacctttttc tgctttgact
      601 tcggcaggat acgctgcgct gcaccagcct cgtcgatgac atcgatcgcc ttgtccggca
      661 ggtggcggtc ggtgatgtag cgggccgcga gttcggccgc cgacgagatc gccgtcgccg
      721 agtacttgat gccgtggtgt gcctcgaagc gcgctttgag gcccttgagg atttcgacgg
      781 tttcggcgac cgagggttca ttgacgtcga tcttctggaa gcgccgggaa agcgcgctgt
      841 ccttctcgaa gatgccgcgg aactcggtgt aggtggtcgc gccgatgcac ttcaactggc
      901 ccgacgagag cgccggcttg agcaggttgg aagcatcgag cgtgccgccg gacgcggagc
      961 cggcgccgat cagcgtatgg atttcatcga tgaacaggat ggcattgggg ttgtcgccca
     1021 actgcttgat gacccctttg aggcgctgct cgaagtcgcc gcggtacttg gtgccggcca
     1081 gcagcgagcc catgtccagc gaatagacgt tggccttggc cagaatgtcg ggaacatcgc
     1141 cctcgacgat gttgcgggcc aggccttcgg caatggccgt cttgccgacg ccggcttcgc
     1201 cgaccagcag cgggttgttc ttgcgccgcc ggcacagggt ctggatgacg cgctcgatct
     1261 ccctgtcacg accgatcagc gggtcgatct tcccctgcag cgccagggcg ttgaggttga
     1321 ttgtgtattg ttcgagagga ccggactgct ccttctcgcc ttcggtttcc acttcgcctt
     1381 ccggtgaact cttttgctgc ggaaccttgg cgatgccatg cgagatgaag ttcaccacat
     1441 cgaggcgcgt gatgccctgt ttctgaagga aatagacggc gtgcgaatcc ttctcgccat
     1501 agatcgcgac cagcacgttg gcgccattga cttccttctt gccggacgat tggacgtgca
     1561 ggatggcgcg ctggatgaca cgctggaagc cgagtgtcgg ctgggtgtcg atgtcgtcct
     1621 cgccggtaac cgtcggcgta tgctcgttga tgaaattggt caggtccttg cgcagcgact
     1681 cgggcttggc gccgcaggag cgcagggctt cggccgccga cgggttgtcg atcagcgcca
     1741 gcagcaaatg ctcgacggtg atgaactcgt gacgcttctg ccgtgcttcg acaaaagcca
     1801 tgtgcaggct gacttcgagt tcctgggcaa tcatcagttt tcctccatga tgcaagcgag
     1861 cgggtgctgg tactggcggg cgaacgcacc tacctgttca accttggtgg cggcgatgtc
     1921 gcgcgggaac cgcccgcata tgccgcgacc ttcattatga actttgagca tgattcttgt
     1981 cgcctgttca atgcccatgg caaaaaaacg ctgaagcacg acgatcacga actccatcgg
     2041 cgtgtaatcg tcgttcagca gcagcacctt gtacatcttc ggaggcatcg ccttggcacg
     2101 ttgtgcttcc agcagactcc cttcctgaga cttcgtagcc atggagcaga ttctatacag
     2161 tccattccat tgtgcaactg ttactggaca tggaaaattc ccggaaagtg ctgcattttc
     2221 cgtagcttat gctgcgccgc gtcaataaaa cttgttgacg cagtgagacg acgggggtag
     2281 aaagcgcttg tgtttggatt cacgttgcat caaggttgcc ttgcgcaccg ggtcgctgag
     2341 ctcaaacagg gagtcgggga gacccgcaat ttattcgctt tttgtaagaa agttgcaaac
     2401 atggcaatcg gtactgtcaa gtggttcaat gattccaagg gctttggctt catcactcct
     2461 gacgatggca gcgaagatct tttcgcacat ttctcggcaa tcaacatgaa tggtttcaag
     2521 accctgaagg aaggcgaaaa ggtttccttc gacgtcgtcc agggccccaa gggcaagcag
     2581 gcgtcgaaca tccagcgcgc ctgagcaacg gataggcagc aactggaagc ccgtctgaca
     2641 ccagacgggc tttttgcttt ccggttccat gaccccgcag tttcacttcg tcaagccggc
     2701 ggggcgcgac gaatgtcaat cgctcgtggt tgcgttggtg gcttcgctgg tgacccagtg
     2761 gccggacttg cctcccgatt tctccagcag gcgaacgttc tcgatacgca tgccgcggtc
     2821 gaccgccttg cacatgtcgt aaatggtcag gagggcgacg gccgtggcgg tcagcgcctc
     2881 catctcgacg ccggtgcggc caaggcattc ggcggtgacg gcgcaatgca cggcattctg
     2941 tgcgacgtcg atcacgaact cggcgctgac ccgcgtcaag gcgaccggat gacagagcgg
     3001 gatcagttcg ctggtccgct tggaagcctg aatagcggca atcctggcga tccccagcac
     3061 gtctcccttg cttgccgacc cggcgcggat gagcgccagc gttgccggct gcatcaagat
     3121 gctgcccgcg gcgcgagcga cgcggaccgt ctcggccttg ccgccgacat cgaccatgtg
     3181 cgcctggccg gccgcgtcga aatgggtaag tgtctgatca gtcacgattt gttacgtttg
     3241 gttacgagtg ggcagcgggg tcgtcaagct gtccaatgta tcatagcaac atgtctgtct
     3301 cccatcgtct gctcgccgga ttcctggcct ttgccctgat cgcgcagcca ctgcgcgccg
     3361 acgaccttcc cgaactgggc gatgtggcga gcaacgaact ctcgctggcg accgaaaaga
     3421 agatcggcca gaagatcatg gacgagattc gctggcgcga accatcttat ctcgacgatc
     3481 cggatgtcga aaactatctg aatcagctcg gtgcgcgtct cgttgccgtc agcaatgatc
     3541 ccggaatggg cttccagttc tttgcgattg atgatatgag cattaacgcc tttgcgatgc
     3601 cgggcggctt catcggcgtc catgtcgggc tgttgctgtc ggcgcagacc gaatcggagc
     3661 ttgccggcgt tctcgcgcat gaaatatcgc acgtgaccca gcgccacatc gcgcgccaag
     3721 tgtaccagtc gaaacagctc tcgatggctt cgatggtggc catggggctc gcgctgcttg
     3781 ccgcgcgctc cagtccgcag ggggccatgg cggcgatcag caccacgcag gcgggggcca
     3841 tatcggcgca gctggctttc tcgcgtgatt ttgaacgcga atccgaccgc ctcggtttcg
     3901 aaatcctgcg ccgcgcgggc tacgatgtgc gcggaatgtc ggcgttcttc gggcggctgc
     3961 aacagtcaac gcgccagtat gagaacaatg ccaccgctta cctgcgaacg catccgctgt
     4021 ccggcgagcg catgtccgac atgcagaatc gcgaacggcc cgtcgcctac cgtcaggttg
     4081 ccgacagccc ggaatttcat ctggtgcggg ccagggtgcg ggcgttgcag gggcgccccg
     4141 tggatgcggt caaggagttc gaggttctct tgcgtgaacg caagtacgcc tccgaggcaa
     4201 gcacgcgcta tggactggcg gtcgctctcg cgcgcaaccg ggactgggcg gctgccgaac
     4261 aggaacttgc ggtggtgcgc aagctcaagg tggcgtcgcc gatggtcgac cgacaactcg
     4321 ccgaggtcaa gatcggccgt ggcgagaagg aggcgggcct gacgctttac cgcgaggcga
     4381 tggtccgcta cccgctgaac atgccgttgc tctacggcta cggcagtgcc ttgctcgccg
     4441 agaaacagtt ttcggctgcc ctcaggtttg ccgacgacca gttgcagagc caccctcagg
     4501 atgcgcgttt cctgaagttg cgggcagatt cctatgccgg tctcgggcgc cgttcgcagc
     4561 atcatctggc actggcggaa ctgaccgctc tcaagggaca gacggccggc gcaatcgaac
     4621 aactgcagct ggcgcagcag gcaggcgatg cgaacttcta cgagatgtcg atgatcgacg
     4681 ggcgtctgcg cgagatgaag aagcggcaga tggaggaaat gaaggaacgg cggaattagg
     4741 ggtaaaatcc gctgtcgtca tattgggtga cccgccatgg aaattgatcg taaccttgat
     4801 gtaaaggggc tcaactgccc gcttcccata ctccgtacca agaaggccct ggctgaaatg
     4861 gagagcggtc aggtgcttcg cgtgcaggcg accgacccgg gttcagtcaa ggatttcgcg
     4921 gcctttgcca aacagaccgg gaatgagctg gtcggg
//
LOCUS       DAORPM010000183         4823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5135_length_4823_cov_9.269924, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000183 DAORPM010000000
VERSION     DAORPM010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5135_length_4823_cov_9.269924"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLD53_08990"
     CDS             <1..758
                     /locus_tag="PLD53_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HRC61090.1"
                     /translation="RTTRRMSPTDEGLAYFERCTQLLADIEETEASVAGESRRPRGRL
                     RLTAPITLGVLRLAPAFAAFSQQYPEITLDIVLSDNVADFTEEGLDMAIRVGRVGSEN
                     VVARRIGETALLIAAAPSYLARAGTPLTPDDLIRHDCFMYSFSATGNHWELEDSNGEP
                     YSVRVGGPIKANNGMLLAEMAVAGSGIVYGPCFILLPLIERDQLVRLLPGCATRRLAI
                     HVVYPTRRHLSARVQAMNSFLSEWFEQRRDAKI"
     gene            764..1549
                     /locus_tag="PLD53_08995"
     CDS             764..1549
                     /locus_tag="PLD53_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC61091.1"
                     /translation="MSDELHKAVVRAESLDPAFDLLAAAYAERLRLQLDGEAAFALQV
                     GHDGLQLVELGAHAAGPIRVDFVAGAVAHRRLFGGGNGQMIAKAVGIQPGVRPVVVDA
                     TAGLGRDAFVLARLGCAMTLIERHPLIAALLEDGLWRALADPVVGPTAAQMHLICANA
                     IDVLRAWDGVPPQVIYLDPMFPHRDKSALVKKEMRLFRPLTGDDDDAPALLDAALALA
                     SHRVVVKRPRKSLAIAGEPPGYVLEGKSSRFDIYPKKSLKAKG"
     gene            complement(1685..2398)
                     /locus_tag="PLD53_09000"
     CDS             complement(1685..2398)
                     /locus_tag="PLD53_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRC61092.1"
                     /translation="MRALIFDVDGTLANTERDGHRPAFNAAFAEVGLPWHWDEAFYGK
                     LLVTCGGFERLRYYAENFDPTTLARPDFDELMHRVHDIKSRNYQRLLAAGAIPLRADI
                     GQLICDARTEGMRLAIASSSKLDNVVGLLRANLGPNVDSWFDVIASGDVAAAKKPSPE
                     LYQWALQQLRLPPRDCLAVEDSERGLAASLAAGIPTVITVSDYTINENFDGARMVLAE
                     AERVSLDKLRLWHATASTS"
     gene            2636..4378
                     /locus_tag="PLD53_09005"
     CDS             2636..4378
                     /locus_tag="PLD53_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC61093.1"
                     /translation="MDCQWACPAHTPVPEYIRLIAAGRYSDAYMINWRSNVFPGILGR
                     VCDRPCEPACRRGRLDQEPVAICRLKRVAADLKDDIHDRLPPAPESRNGKRIACVGAG
                     PASLTVARDLAVLGYQITVFDNGASPGGMMRSQIPKFRLPDDVIDEECAYIVNLGVET
                     RYNQWINSLRDLLAEDWDAIFVGTGAPRGRDADIPGRQEAAAHIHIGIDWLANVAFGH
                     TTRVSPRVIVLGGGNTAMDCCRSALRMGGEDVKVVVRSGFDEMKASPWEKEDAAHEGI
                     PILNMLVPKEFVHNNGQLSGVLFEKVRAEYDDKGRRSLIPTGEADILMECDEVLVAIG
                     QENAFPWIEKDIGLEFDKWGLPVLNQATLQSTLPKVFFGGDSALGPKNIITAVAQGHD
                     AAISIDLFCAGKDLLERPPPLTNLVSQKMGIHEWSYDNQVSEEERKKVPVKSLEKTLK
                     DIKLELELGFDPYMACSEAERCLNCDIETVFTAKTCIECDSCVDICPTECITFTGNGE
                     EDDLRSRLKAPADNLDQALYVSDILKTGRVMVKDENICLHCGMCAERCPTGAWDMQKF
                     FLQMAEAGTEVRRR"
     gene            4375..>4823
                     /locus_tag="PLD53_09010"
     CDS             4375..>4823
                     /locus_tag="PLD53_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRC61094.1"
                     /translation="MSAIKSIQRSNDFVIKFANVNGSGSASANELFARAIMNMGVPVA
                     PRNIFPSNIQGMPTWYEMRVSESGWLGRRGGCDLMVAMNPQTWDRDVAELESGGYLVY
                     DSTKTLPRSRFREDITVLGVPLTEICNREYTDPRQRQLFKNIMYVGAL"
BASE COUNT          962 a         1373 c         1506 g          982 t
ORIGIN      
        1 atcgcacgac gcggcgaatg agcccgaccg acgaaggttt ggcttacttc gagcgctgta
       61 cacagctgtt ggcggatatc gaagagaccg aagccagtgt ggctggcgaa tcacggcgcc
      121 cgcgcggccg cctgcggcta actgcgccaa tcaccctcgg cgtacttcgg ttggcgccgg
      181 cctttgctgc tttctcacag cagtatccgg agatcacact cgatatcgtt ctttcggata
      241 atgttgccga tttcaccgaa gaggggttgg atatggccat ccgggtcggg cgagtcggca
      301 gcgaaaatgt cgtcgctcga cgcatcggcg aaacggcgct gctgatcgcc gccgctccgt
      361 cttatctggc gcgggcaggg acgccgctga ctccggacga tctaatccgg catgactgtt
      421 tcatgtattc gttttcggca accggaaacc actgggagct ggaagattcc aacggtgagc
      481 cgtacagcgt acgcgtcggc gggccgatca aggccaacaa cggcatgctg cttgcagaaa
      541 tggcggtggc cggtagcggg atcgtgtatg gcccgtgctt catcctgcta ccgttgatcg
      601 agcgtgacca actcgttcgc ctgctaccgg gctgtgctac ccgccgacta gcgattcacg
      661 tggtgtaccc cacacgacga cacctgtcgg cgagggtgca agcaatgaac agtttcctca
      721 gtgagtggtt tgaacagcgg cgcgacgcga aaatatagcc gcaatgagcg acgagttgca
      781 taaagccgta gttcgagctg aatcgctaga ccccgcgttt gatcttttgg cggcagccta
      841 tgcagagcgg ctccggttgc agctcgatgg cgaggcagcg tttgccttgc aggtcggtca
      901 cgatggcctg caattggtcg agctgggcgc gcacgcggcg gggccgatcc gggtggactt
      961 tgtcgccgga gcagtagcac accggcgatt gttcgggggc ggcaacgggc agatgatcgc
     1021 caaggctgtg ggcatccagc ccggagttcg tccggtggtg gtggatgcca cggccgggct
     1081 ggggcgcgat gccttcgtgc tggcccggct gggttgcgcg atgaccctga tcgagcgtca
     1141 ccccttgatt gccgcattgc ttgaggacgg cttgtggcgt gcgctggcgg atccggtggt
     1201 cggaccgact gccgcacaga tgcacctgat ctgcgccaat gccattgatg tcctgcgcgc
     1261 ttgggatggt gtgccgccgc aagtcattta tcttgatccg atgtttccgc atcgcgacaa
     1321 gagcgcgctg gtgaagaagg aaatgcgact ctttcgtcct ttgaccggtg atgatgatga
     1381 cgcacccgcg ctgcttgatg cggcgctggc gctggcgtcg catcgagtgg tcgtcaaacg
     1441 gccgcgcaag tcgctggcca ttgctggcga accgcccggc tatgtgctgg aaggcaaatc
     1501 cagccgattc gacatctacc cgaaaaagtc gttgaaggcg aaagggtaag aacaggagcg
     1561 aaccaccaag tcacagagac accaagtccc accaagaaaa cctaaacaaa gattttgact
     1621 ttcttggtgc ttcttcgtgt acttggtgac ttggtggttc gcctttctcg tcaacgatct
     1681 ctgtctatga cgtactcgcc gtcgcgtgcc acagacgcag tttatccagc gaaacccgtt
     1741 cagcctcggc cagcaccatg cgcgcgccgt cgaaattctc attgatcgtg tagtcactga
     1801 cggtaatgac cgtcggaatg ccggccgcaa gactcgccgc cagtccacgc tcggaatcct
     1861 ccaccgccag gcaatcacgc ggtggcagcc tgagttgctg cagcgcccac tggtaaagtt
     1921 ccggagacgg tttcttggcc gctgcaacgt cgccggaggc gatgacatcg aaccaggaat
     1981 ccacattcgg gcccagattg gcgcgcaaca gacctaccac attatccagc ttcgagcttg
     2041 aggcgatggc cagccgcata ccttcggtac gcgcatcgca aatcagttga ccaatgtcgg
     2101 cacgcagcgg aatcgctccg gcagccagca aacgctgata attccgcgat ttgatgtcat
     2161 gtacccggtg catcagctcg tcgaagtcgg gccgggccag cgtcgtgggg tcaaaattct
     2221 cggcgtagta gcgcagccgc tcaaatccgc cgcaggtgac cagcaacttg ccgtagaacg
     2281 cttcgtccca gtgccagggc agcccgactt cggcaaaggc ggcattgaac gcggggcgat
     2341 gcccgtcgcg ctcggtgttg gcaagcgtac catcgacatc aaaaattaac gcgcgcaagg
     2401 ccatgctgga ttacccccgt ctagagacat ggtgccaaga ctagccgatt tttatcgtgc
     2461 aggaaaactg aattcctttc caggttggtc ggcagacgtt ttgctttggc ttggacggtg
     2521 ctagagtgcg gggaggcggc ttcttgttgc cgatccttat gtagccaccg tgacaggaga
     2581 ctgagcacac gtgataccga cagatatcgg ccaacacgat tactttcaca gagtcgtgga
     2641 ttgtcagtgg gcgtgtcctg cccacacccc cgttcccgaa tacatccgcc tgattgcagc
     2701 gggccggtat tcggatgcct acatgatcaa ctggcgatcc aacgtctttc cgggcattct
     2761 cgggcgggtc tgcgatcggc cgtgcgagcc ggcctgccgt cgcggccggc tggaccagga
     2821 accggtagcc atctgtcggc tgaagcgcgt tgctgccgac ctgaaggatg atattcacga
     2881 tcgcttgccg cccgcgccgg agagcaggaa tggcaaacgc atcgcctgcg ttggcgccgg
     2941 ccccgcttcg cttaccgtgg cgcgcgatct ggcggtgctg ggctatcaga tcaccgtttt
     3001 cgacaatggc gcatcgcctg ggggcatgat gcgcagccag atcccgaaat tcaggctgcc
     3061 ggacgacgtc atcgacgaag agtgtgccta catcgtcaat cttggcgtgg aaacgcgcta
     3121 caaccagtgg atcaatagct tgcgggatct gctggccgaa gactgggatg cgatctttgt
     3181 cggcactggc gccccgcgtg ggcgcgatgc ggacattccc ggtcgtcagg aggcggctgc
     3241 tcatatccac atcggcatcg attggctggc caatgtagcc tttggtcaca ccacccgcgt
     3301 ttctccgcgc gtgatcgtgc tcggcggggg caatacggcc atggactgct gccgctcggc
     3361 acttcgcatg ggcggcgagg atgtcaaggt tgtcgtgcgc agcggcttcg acgaaatgaa
     3421 ggcgtcgccc tgggagaagg aagacgcggc gcatgagggt atcccgattc tcaacatgct
     3481 ggtgccgaag gagttcgtgc acaacaatgg gcagctttcg ggcgttctgt tcgagaaggt
     3541 ccgggccgag tacgacgaca aaggcaggcg gagtctgatt cctaccggcg aagcggatat
     3601 cctgatggaa tgcgacgagg ttctggtggc catcgggcag gagaatgcct tcccttggat
     3661 cgagaaggac atcggacttg aattcgacaa gtggggactg ccggtattga atcaggcgac
     3721 gctgcaatcg acgttgccca aggtattttt cggcggcgac tcggctctgg gtccgaagaa
     3781 catcatcacc gccgtggcgc aggggcatga cgccgcaata tcgatcgatt tgttctgtgc
     3841 cggcaaggac ctgctggaac gcccgccgcc actgaccaat ctggtcagcc agaagatggg
     3901 cattcacgag tggagttacg ataaccaggt ctccgaggag gagcggaaga aggtgccggt
     3961 gaagtccctg gagaagacgc tcaaggacat caagcttgag cttgaactgg gcttcgatcc
     4021 gtacatggcc tgctccgagg ccgagcgttg tctcaattgc gatatcgaga cggtatttac
     4081 cgccaagacc tgcatcgaat gcgattcctg cgtcgatatc tgccccaccg aatgcattac
     4141 cttcaccggc aatggcgaag aggatgactt gcgaagccgt ttgaaagcac cggcggataa
     4201 cctggatcag gcgctttatg tttcggatat cctgaaaacg ggacgcgtca tggtcaagga
     4261 tgaaaacatc tgcctgcatt gcggcatgtg cgccgagcgt tgcccgacag gcgcctggga
     4321 tatgcagaag tttttcctgc aaatggcgga agccggaacg gaggtgcgaa gaagatgagt
     4381 gcgatcaagt cgattcagag aagcaatgat ttcgtcatca agtttgccaa cgtcaacggt
     4441 tccgggtcgg catcggccaa cgagctgttt gcgcgcgcca tcatgaacat gggggtgccg
     4501 gtggcgccgc gcaacatctt cccgtccaac attcagggca tgccgacctg gtacgaaatg
     4561 cgcgtttcgg aatccggttg gctgggtcgg cgcggcgggt gtgacctgat ggtggcgatg
     4621 aacccgcaaa cctgggatcg cgacgttgcc gagctcgagt ccggcggcta tcttgtctac
     4681 gattcgacca agaccttgcc tcgctcgcgg ttccgcgaag acattaccgt gctgggcgtt
     4741 ccgctcaccg aaatctgcaa tcgcgagtac accgatccgc gccagcgcca gttgttcaag
     4801 aacatcatgt acgtcggggc gct
//
LOCUS       DAORPM010000184         4810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5156_length_4810_cov_10.812618, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000184 DAORPM010000000
VERSION     DAORPM010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5156_length_4810_cov_10.812618"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLD53_09015"
     CDS             <1..584
                     /locus_tag="PLD53_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRC61095.1"
                     /translation="MAEGKILPYLDVPFQHASPRVLKAMKRPASAENNLERIKAWRTI
                     CPDITLRSTFIAGFPGETESEFEELLAFIEEARLDRVGCFAYSPVDGASANTLAGMVP
                     DEVREERRRRLMDVQEDISAELLAAKIGREIKVLVDEVDEEGTIARSSADAPEIDGLV
                     FINDFFSAEPGDFLSVKVIDADEHDLYAELVNT"
     gene            724..2133
                     /locus_tag="PLD53_09020"
     CDS             724..2133
                     /locus_tag="PLD53_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRC61096.1"
                     /translation="MTQGSLIEELAAIVGAENVLTEPDDTAPYLLDWRGRYRGTARCV
                     VRPSTTEAVSAVVRVCAQAGVAIVPQGGNTSLCGAGIPDESGETVLISLSRLNRIRAI
                     DSANNTIIIEAGCVLQTLQEAAAQAGRLFPLSLAAEGSCQIGGNLSTNAGGVHVMRYG
                     NMRELALGLEVVLPSGEIWNGLRGLRKDNSGYDLKQLFIGAEGTLGIITAAVLKLFPL
                     PQSVATAWMAISTPSAAVRLLGELQGAFGTGLNACELVSDIALGLVLKHIPGAQAPLA
                     RSPWHLLVEVSGESEEGVLHAALEGFLEQALKNGAISDAVLAQNSAQARRLWALRENI
                     SEAQKIEGLSIKHDLSVPISYIGEFLDRADAALKQAFPSYRIVAFGHVGDGNLHYNQS
                     KPETMENDAFIAAQSQINRIVHDIVNDLGGSISAEHGLGQLKREEILRYKSPLEMEMM
                     RAVKRALDPQGLMNPGKVL"
     gene            2306..3160
                     /gene="folD"
                     /locus_tag="PLD53_09025"
     CDS             2306..3160
                     /gene="folD"
                     /locus_tag="PLD53_09025"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRC61097.1"
                     /translation="MTAQLLDGNALGQKLRAGFKHKAEELAAQGTRPGLAVILVGGDQ
                     ASQVYVRNKVNACAQAGIHSEKYSYDDDVAPQVVLDKIAALNADPKIHGILVQLPLPK
                     HFDADAVLEAIAPEKDVDGFRAENVGALVQGQACFIPCTPYGVMKFFEEAGVNLKGKE
                     AVVVGRSNIVGKPMAMLLMHAGATVTVCHSQTRDLKGHCQRADILVAAIGKPKMITCD
                     MIKPGAVVIDVGINRQPDGKLCGDVDFDSAKDVASFITPVPGGVGPMTITMLLANTLE
                     SAEHFAST"
     gene            3165..3668
                     /gene="purE"
                     /locus_tag="PLD53_09030"
     CDS             3165..3668
                     /gene="purE"
                     /locus_tag="PLD53_09030"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRC61098.1"
                     /translation="MNMNKQPLVGIVMGSDSDWPVMRSCAETLKRFGIAYEAKVLSAH
                     RTPDAALDYASSAQGRGIKVLIGAAGGAAHLAGVLAAKSELPVLAVPMPSKHLQGLDS
                     LLSMVQMPGGIPVATFAIGEAGAVNAALFAVSMLALSDPDLAKKLTDFRFNQSEKVLS
                     KTLPDLP"
     gene            3673..4701
                     /locus_tag="PLD53_09035"
     CDS             3673..4701
                     /locus_tag="PLD53_09035"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRC61099.1"
                     /translation="MSQEFARAVELLQAGELVAFPTETVYGLGADAANPEAVAKVFAA
                     KGRPADHPLIVHLPGAAYLDRWASGIPAVAWELVEAFWPGPLTLILKRTPEVPYAVTG
                     GQETIGVRVPAHPVALELLRTYAQAGGSRGGMCGIAAPSANRFGRISPTTAAHVRQEL
                     GDSVALVLDGGQCPVGIESTIIDLSRDGGLPPRLLRPGHISPEEIASVIGVLPALPEA
                     GVGVEVPRVSGALEAHYAPQTLMRLVVTEQVPAVICEMQGNDRRCGLLSRTRQSFVQM
                     PHSQRHLPDEPKGFARGLYAALRELDEQGNDLIVVENVPNDSAWAAVADRLRRAARGS
                     AETDFHHL"
BASE COUNT          955 a         1336 c         1493 g         1026 t
ORIGIN      
        1 tgatggccga aggcaagatt ctgccttatc tcgatgtgcc gtttcagcac gcaagtccac
       61 gcgtcctcaa ggccatgaag cgtccggcca gtgccgaaaa caaccttgag cgcatcaagg
      121 cgtggcgtac catctgcccg gatatcaccc tgcgcagcac cttcatcgcc ggattccccg
      181 gtgaaaccga gtccgagttt gaagaactac tggccttcat cgaggaggct cgacttgatc
      241 gggtgggctg ctttgcctat tcgccggttg atggcgcgtc ggccaatacc ctggcgggta
      301 tggtgccgga cgaggtgcgc gaagagcgcc gccggcgtct gatggacgtt caggaagaca
      361 tttccgccga gttgctcgcc gccaagatcg ggcgtgaaat caaggtgttg gtcgatgagg
      421 tcgatgagga agggacaatt gcgcggtctt cggcggatgc gcccgagatc gacggtttgg
      481 tcttcatcaa cgactttttc agcgccgagc cgggcgattt cctgagcgtg aaagttattg
      541 acgcggatga gcacgatctt tatgcggagc ttgtaaatac ttagccatgg cgtgtagcaa
      601 gcgcgccaga gaaagttaat atcgcgggtt tggaaacaac ccgtggaaga tcgcaaggat
      661 ctgttcttca cttgacgtgt ttgtcttgtt gacgaaactg tcggccttat caggagtgtg
      721 agcttgaccc aggggagtct gatcgaagaa ctcgctgcca ttgtcggtgc cgaaaacgtc
      781 cttacagagc cggatgatac tgcgccgtat ctcctcgact ggcgcgggcg ctatcgcggc
      841 acggcccgct gcgtagtgcg tccgtcgacg accgaagcag tgtcggccgt cgtgcgcgtc
      901 tgcgcgcagg ccggtgtggc aattgtccct cagggcggaa ataccagcct gtgcggtgcg
      961 ggaattcccg atgaaagcgg agagaccgta ctgatcagcc tgtcgcgcct gaaccgaatc
     1021 cgggcgattg attctgccaa caacacaatc atcatcgaag ccggctgtgt tcttcagaca
     1081 ttgcaggagg cggcagcgca ggccggaaga ttgttcccgc tttcattggc tgcagaaggc
     1141 agttgccaga tcggcggcaa tctctcgacc aacgccggcg gagttcatgt gatgcgctac
     1201 gggaatatgc gcgaacttgc actcggcctg gaggttgtac tgccgagtgg cgaaatctgg
     1261 aatggcctgc gcgggctgcg caaggataac tccggatatg acctcaagca gttgttcatc
     1321 ggtgccgagg gaacgctggg catcatcacg gcagcagtat tgaagctctt tccgcttcct
     1381 caatccgtcg ctaccgcctg gatggcgatt tcaacacctt cagcagcggt acgccttctc
     1441 ggtgaacttc agggcgcttt tggcacagga ttgaatgcct gtgaactggt ttccgatatc
     1501 gccctgggtt tggtgctcaa acatattccg ggcgcccagg cgccgcttgc tcggagcccg
     1561 tggcatctgc ttgtcgaggt ttcaggtgag agcgaggagg gggtactgca cgctgcgctg
     1621 gagggcttcc tcgaacaagc gctgaagaat ggtgcgatca gcgacgccgt gctggcgcag
     1681 aacagtgcac aggccaggcg cttgtgggcg ttacgcgaga atatcagcga ggcccagaag
     1741 atcgagggct tgagtatcaa gcacgacctt tcggtgccga tttcgtacat cggcgagttt
     1801 ctcgatcgcg ccgacgcggc gctgaagcag gccttcccca gctatcgaat cgttgctttc
     1861 ggacatgtgg gcgacggcaa cctgcactac aaccagtcaa agcccgagac gatggagaac
     1921 gatgcattca ttgcggcgca gtcgcagatc aatcggatcg ttcacgacat cgtgaatgat
     1981 cttggcggca gcatcagcgc cgagcatggt cttggccaac tcaagcgtga ggaaatactg
     2041 cgctataaaa gtccgcttga aatggagatg atgcgtgctg tcaagcgggc actggatccg
     2101 cagggattga tgaatcccgg gaaagtgctt tgattgagtt gatagttgga ccgtgtgtct
     2161 cgattgcact tcgggatggt gattccgtcg gctgcggagg ccgaagtgtc ctgagttgcg
     2221 gtaaaatctc cggctctttc aagaggaatg caagagacgc aagcaactcg ctcggtcctt
     2281 cctttcccgt tttccggaaa tcatcatgac tgcacaacta ctcgacggta atgcgctcgg
     2341 acaaaaattg cgcgccggtt tcaagcacaa ggctgaggaa ctcgcggcgc agggaacgcg
     2401 tcccggcctc gccgttatcc tggtcggtgg cgatcaggct tcgcaggttt acgtccgcaa
     2461 caaggtcaat gcgtgtgccc aggccggtat tcattccgaa aaatacagct acgatgatga
     2521 cgtagcgcct caagtggtgc tcgacaagat cgcagcactc aacgccgacc caaaaattca
     2581 cggtattctt gtccagttgc ctttgcccaa gcattttgat gccgatgcag tgctggaagc
     2641 cattgcgcct gaaaaagacg tcgacggatt tcgtgccgag aatgtcggag cgctggtgca
     2701 ggggcaagcc tgcttcattc cgtgcacgcc gtatggcgtg atgaaattct ttgaggaggc
     2761 cggagtgaat ctcaagggca aggaagccgt tgtcgtcggc cgctcgaata tcgtcggcaa
     2821 gccgatggcc atgctgctga tgcacgccgg cgctacggtc acggtatgcc actcgcaaac
     2881 gcgggatctc aagggtcatt gtcagcgtgc ggatatcctt gtcgcggcga taggaaagcc
     2941 gaagatgatt acctgcgaca tgatcaaacc tggcgcggtt gtcattgacg ttggaatcaa
     3001 ccgtcagcct gacggaaagc tttgcggtga tgtcgatttc gactcggcga aggacgtcgc
     3061 atcgttcatc acgccagtcc caggtggtgt cggcccgatg acgattacca tgctattggc
     3121 caatacgctc gagagcgccg agcactttgc aagcacttag ggagatgaac atgaacaaac
     3181 aaccgctcgt cggcatcgtg atggggtcgg atagcgactg gccagtaatg cgcagctgcg
     3241 ccgaaacgct caagcgcttc ggcattgcct acgaagccaa agtgctttca gcgcatcgta
     3301 cgcccgatgc ggcgctcgat tatgcgtcca gtgcgcaggg gcgtggcatc aaggtgctga
     3361 tcggcgccgc tggcggtgcg gctcacctcg ctggcgtgct ggcggccaag agcgaattgc
     3421 cggtgctggc ggtacccatg ccatccaaac acttgcaggg gctcgattca ttgctgtcca
     3481 tggtgcagat gcccggcgga ataccagtcg ctacttttgc catcggcgaa gccggagcag
     3541 taaatgcggc gttgtttgcc gtatcgatgc tggccctgag cgatcccgat ctggccaaga
     3601 agctgactga tttccgcttc aatcagtccg aaaaggtgct gtcgaagact ttgccggact
     3661 tgccttgaag aaatgagtca ggagttcgcc cgagccgtcg agctgctaca ggccggcgaa
     3721 ctggtcgcct tcccgaccga gacggtctac ggcttgggtg ccgatgcggc gaaccccgag
     3781 gctgtagcta aagtatttgc ggccaagggg cggccggcag accatccact gattgtgcac
     3841 ttgcccggcg cggcctatct tgatcgctgg gcgagcggta ttcccgctgt ggcctgggaa
     3901 ctggttgaag cgttctggcc aggcccgctc accctgattc tcaagcgcac acctgaagtt
     3961 ccctatgcgg tgacgggagg acaggaaaca atcggcgtac gcgtgccggc acatcccgtt
     4021 gcgctcgaac tgctgcgcac ctacgcgcag gccggtggaa gccgcggcgg catgtgtggc
     4081 attgctgcgc catcagccaa ccgtttcggg cgtatcagcc cgaccactgc tgcgcacgtg
     4141 cggcaagagc tgggcgattc cgttgcgctg gtgcttgacg gcggacagtg tccggttggc
     4201 attgaatcga cgatcattga cctgtcgcga gatggtggcc tgccaccgcg cttgctccgc
     4261 ccagggcaca tctcacctga ggagatcgct tcggttatcg gcgtgctgcc cgctctgccg
     4321 gaggcaggcg ttggcgtgga ggttccacgc gtatccggcg cgctggaggc gcactatgcg
     4381 ccacagacgc tgatgcggct ggtcgtgacc gaacaagtgc cggcagtcat ctgcgagatg
     4441 caaggcaatg atcggcgctg cggacttctg tcgcgaaccc gtcaatcctt cgtgcagatg
     4501 ccccactcac agcgccactt gccggatgaa ccgaagggtt tcgcgcgtgg cctgtatgcg
     4561 gccctgcgcg agcttgatga acagggcaac gatcttatcg tcgtcgagaa tgttcccaac
     4621 gattcagcct gggccgccgt ggctgaccgc cttcgccgcg cggccagagg ttctgctgag
     4681 acggattttc accacctgtg atttgtgcga ccttcgatca gcgaattctg ttcttggtag
     4741 cttgacgtgc ccgttatcga gcgtttcttc gcatcatttc ggctgccatg gcggtgaatt
     4801 gctcctcgac
//
LOCUS       DAORPM010000185         4806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5167_length_4806_cov_11.283098, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000185 DAORPM010000000
VERSION     DAORPM010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5167_length_4806_cov_11.283098"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..476)
                     /locus_tag="PLD53_09040"
     CDS             complement(216..476)
                     /locus_tag="PLD53_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61100.1"
                     /translation="MSHQLSGVALAAAAAALFTAGAVMAPVAQAAEGGMVHCTGINSC
                     KGHAECKTAKNECKGMNACKGQGWVAKASAKECTDAGGKVAK"
     gene            659..1228
                     /locus_tag="PLD53_09045"
     CDS             659..1228
                     /locus_tag="PLD53_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRC61101.1"
                     /translation="MQQYKRLLAVALFLGTLLAVFELSGLRDHFSLTFLQQQIAENRV
                     SGLLVFVLLFSLGNLIQIPGWVFLAAAVLTLGRIWGGLATYVAASISCALTFFTIRLV
                     GGDVLRQLKSKTAVRILGQLDARPIRSVVLLRVLFQTVPALNYALALSGIRFRQYFTG
                     TLLGLPLPIILYCLFFDYLAKMLRIPGVQ"
     gene            complement(1263..1661)
                     /locus_tag="PLD53_09050"
     CDS             complement(1263..1661)
                     /locus_tag="PLD53_09050"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRC61102.1"
                     /translation="MQLTIIAAILISILGVAFAMQNNVPVTVNFLLWRLDSSLAMVLL
                     LALALGAVIIALLTTPATLRWQWLTKRQKRRIEELEKNCDGQRRRIAELESHLPPETD
                     AAEPRPYAGLMQLIGSREDEAVGESKQPPV"
     gene            complement(1678..2889)
                     /locus_tag="PLD53_09055"
     CDS             complement(1678..2889)
                     /locus_tag="PLD53_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61103.1"
                     /translation="MTYQAILAASIFLLIAFGLERLGRASGIPSAILLIAAGLAGKPV
                     LETFGLALEGISAAVPIVGTLGLILIVLEGTLDIKLRRDRLRAAAGAFLIAGGGFVVC
                     TTVFAFAGSLILNLTPLQSVILAVPFAVISSTIAIPSSSFLPPWGREFVIYESSFSDI
                     LGVLVFFSLLNSNGTLKGALLGMIGGGALSLLLALVCAVGLVLILMRIDGHIRFTPLL
                     AGLFGLYAAGKLMHLSPLILVLLFGLLLNNPTLIVRFRPFRGWLDERYEETLDEFRLL
                     ILELTFAVRGFFFILLGYWTNLSELVSLKAWLAATLILTVVYGSRYLMLRLARFDLLG
                     PLTCMAPRGLITVLLFLSAKEALPLPHYFFGTVMLVVLVSATLITIGRYRWADVGVFP
                     ASSNARKPESI"
     assembly_gap    3063..3162
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3163..3653)
                     /locus_tag="PLD53_09060"
     CDS             complement(<3163..3653)
                     /locus_tag="PLD53_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61104.1"
                     /translation="MTAVQMVNTLASFLTRHIEQRFRVLAWVFGVIAVATLGVLHTAT
                     ETDYAFSSAVIIPVVAVAWTGGRRDGFSFSLLAALLWVSADVLAERQFSSGWVPLING
                     LTRFATYGFVTYLIVEVQTLLARVQEMARHDTLTTLLNRGAFFEAGDAEASRCQRYGH
                     HIAV"
     gene            complement(3666..4484)
                     /locus_tag="PLD53_09065"
     CDS             complement(3666..4484)
                     /locus_tag="PLD53_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HRC61105.1"
                     /translation="MRWLLALFRLIFRVVRRFQTEHLTLTSAALSFATLLGLVPMIAV
                     GLGLISHFPFAENVSVALERFLLANLLPDKAGAVIAKYLGQFAHRAGGIPLMGGIALV
                     VTALMQMLTIEHAFNTIWKIKTKRPLYRRLAMHLIALLLGPVAFGVSLALISVVAGVS
                     SGLLDEPVWVSVTFFRVLPFMITAALFSFLYWGVPNRAVSRWHAMSGGVLAALGFVAM
                     QRLFGLYVANFPNYAVMYGAFSAVPIFLAWLYFSWGVILAGALVVAELPYVAQR"
BASE COUNT         1157 a         1370 c         1287 g          892 t
ORIGIN      
        1 ccgccatcga ccatgtagtt ttcggagatt atttcgagcc agtcgacccg ctgtggggct
       61 gagacaaagt cgctgtagtg ctcgacccgg agtccaagac catagccttg aattggttta
      121 ctcaacgacg tgctcaacgg catcttccct gaaacctcga aggattgtgg ctgataaggc
      181 cgccccgtgc tgcaacaggg cggcccgcat cagtgttact tggcaacctt gccgccagcg
      241 tccgtgcatt ccttggcact ggctttggct acccaaccct ggcccttgca ggcattcatc
      301 cccttgcatt cgttcttggc cgttttgcac tctgcatgac ctttgcacga attgattccg
      361 gtgcaatgaa ccataccgcc ttctgcagct tgtgcaacag gtgccatgac ggcgccagcg
      421 gtaaaaaggg cagccgctgc agcggccaga gcaacacctg aaagttgatg tgacattttg
      481 gttctcctgg ttatgatggg gtggtggcga agcctgccga cttgaaacca acttgtcgac
      541 acccgttaga cgctggaaaa tggcaatcct tacataaatt ctgaaaaatt cttttcaaga
      601 cagcacaaat tatcgaactc gtatcgctcc gaacaacgaa aacacaacga aatgtgtcat
      661 gcaacaatat aaacgactgc tcgcagttgc ccttttcctg ggcacgttgc tcgccgtatt
      721 tgagctttcc ggactgcgtg atcacttcag tctgacgttt ctgcagcagc agatagccga
      781 aaatcgcgtc agcggcttgc tggtcttcgt cctgcttttt tcgttgggca atctgatcca
      841 gatcccgggc tgggtgtttc tggccgccgc cgtgctaacg ctcggtcgaa tctggggcgg
      901 gctggccacc tatgtcgccg ctagcatttc gtgcgctctg acttttttca ctatccggct
      961 ggtcggtgga gatgtcctgc gacaattgaa gagcaaaacc gctgtccgga ttctcgggca
     1021 gcttgatgcc cgaccgatca gaagtgtcgt actcctgaga gtgctgtttc aaaccgtacc
     1081 cgccctcaac tacgccctcg ccctgtcagg catcaggttc cggcaatact tcaccggcac
     1141 gcttctcggt ctgcctttgc cgatcattct gtattgtcta ttttttgact acctggccaa
     1201 aatgctgagg attcctggcg tccaataggc accgctctaa tcgttgcagt gaccgcgctt
     1261 tctcaaaccg gaggttgttt cgattcgcca accgcttcgt cctcccgact gccgatcagc
     1321 tgcatgagac ctgcgtatgg gcgtggttca gctgcatcgg tttcaggtgg gagatggctt
     1381 tcaagttcgg caatccggcg gcgctgaccg tcgcagtttt tctccagctc ctcgatccgg
     1441 cgtttctgtc gcttcgtcag ccattgccat ctgagcgttg ctggcgtggt caacagggcg
     1501 atgatgacgg ccccaagcgc aagggccagc agcagcacca tggccaagga actgtccaaa
     1561 cgccaaagca gaaagttgac ggtaaccggc acgttgttct gcatggcaaa ggcgacgcca
     1621 agaatagata tcaggatggc cgcgatgatg gtcagttgca tggagttctc cttccgctta
     1681 gatggattcc ggctttctcg cgttcgagga tgcagggaaa acgcctacgt cggcccaacg
     1741 gtaacgccct atggtaatca aagtagccga aaccaggacc accagcatca ccgttccaaa
     1801 aaaataatgt ggcagcggca atgcctcctt ggccgacaga aaaagcaaca cggtaataag
     1861 tccgcgcgga gccatgcagg tcagcggccc cagaaggtcg aaacgagcca gtcgcagcat
     1921 gaggtaacgg ctaccgtaga ccacggtcag aatcagtgtt gccgccagcc aggctttcag
     1981 cgataccagc tcactcaagt tggtccagta gcccagcagg ataaagaaga aaccacgcac
     2041 agcgaaggtc agctccagga tcagaagtct gaactcgtcg agtgtctcct cgtaacgctc
     2101 gtcgagccaa ccccgaaaag ggcgaaagcg aacaatcagc gtgggattat tgagcaacag
     2161 gccgaacagc agaaccagaa tcaatggtga caagtgcatc agcttgcctg ccgcgtagag
     2221 accgaagagt ccagccagca agggcgtaaa acgaatatgg ccgtcaatgc gcatgaggat
     2281 cagaaccagg ccgacggcgc aaacaagcgc cagaagcagc gagagtgcac cgccaccaat
     2341 cattcccagg agggcgcctt ttagggttcc gttggagttg agtagcgaga aaaagaccag
     2401 cacacccaga atgtccgaga aggagctctc ataaatcacg aactctcggc cccatggcgg
     2461 caggaaacta ctgctcggga tggctatcgt actgctgatg actgcgaaag gcacggccag
     2521 gatcacggat tgaaggggtg tcaggttcaa gatcagggac cctgcgaacg caaacactgt
     2581 agtgcagacg acaaaaccgc cgccggcaat caaaaaggcg ccagctgccg cgcgcaaacg
     2641 atcccggcgg agtttgatat ccagggttcc ttcaagcacg atcaaaatca agcccagggt
     2701 accgacaata ggcaccgcag cgctaatgcc ttccagcgca agaccgaaag tctccagcac
     2761 cggtttgcca gcgaggccgg ccgcgatcag gaggatggct gaaggaatgc ctgaggcacg
     2821 gcccaagcgc tcaaggccaa aggcgatgag caaaaagatg gatgcggcga gaatggcctg
     2881 ataagtcaat ttggttccga ttctgtgggt gggtcgggtg gcactaccgc gccacgccgt
     2941 gtccgaacgc catgcttgcc gtcccgcttg atttcgtaca tcagggcatc ggcggagccg
     3001 agcatagcgg gaaagccaca ctcggcgttc tcgaaccagg cgatcccgac gctgacgctg
     3061 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacggcgat gtgatggccg
     3181 tagcgctgac agcggctggc ctcggcatcc cccgcctcaa aaaaagcgcc ccgattgagc
     3241 aacgtcgtca aggtatcgtg gcgggccatt tcctgcaccc gagccagcag cgtttgaacc
     3301 tcaacgatca gatacgtgac aaagccatag gtcgcaaacc gggtcaggcc attgatcaat
     3361 ggcacccagc cagagctgaa ctgacgttct gccagcacat cagcactcac ccacaagagc
     3421 gccgccagca acgagaaaga gaagccgtcc cgccgcccgc cggtccaggc aacggctacc
     3481 acgggaatta tgacggccga ggaaaaggcg tagtcggtct cggtggcggt gtgcaataca
     3541 ccaagggttg caaccgcgat gacgccaaaa acccatgcga gcaccctgaa tcgttgctcg
     3601 atatgtctgg tcaggaaaga agcgagagtg ttcaccattt ggacggcagt caagctttgt
     3661 tcgttctagc gttgcgccac gtacggcaat tccgcaacca ccagtgcgcc cgcgaggatg
     3721 actccccagg aaaagtataa ccaggcaaga aagatgggca ccgcagaaaa agccccgtac
     3781 atcacagcgt agtttggaaa gttggcaaca taaaggccga acaaccgctg catggcgaca
     3841 aagcccaggg cggcgagcac accaccgctc atcgcgtgcc atcgcgatac cgcacggttc
     3901 ggaacacccc agtaaaggaa agaaaagagt gctgcggtaa tcatgaatgg cagaactcga
     3961 aaaaaagtaa cgctcaccca cacgggctcg tcgagaagcc cggaagaaac accagcaacc
     4021 acggagatca gggccagact tacgccaaac gcgaccggcc cgagcaacaa cgctatcagg
     4081 tgcatcgcca agcgcctgta caaaggacgc ttcgtcttga tcttccagat tgtattgaac
     4141 gcatgctcga tggtcagcat ctgcatcagc gcggtgacca ccagcgcgat gccgcccatc
     4201 aggggaatgc cgcctgcacg atgagcaaac tggccgagat acttggcgat aacggctcca
     4261 gccttgtccg gcaaaaggtt ggcgagcaga aatctttcga gagccacact cacattctcc
     4321 gcaaagggaa aatgcgatat caaaccaaga ccgacggcaa tcatcggcac cagcccgagc
     4381 aaggtggcaa acgacagcgc tgccgaggtc agtgtcaagt gttccgtctg gaaacggcga
     4441 acgacgcgga atatcagccg aaaaagcgcg agcagccagc gcacgggatc agcgcacttt
     4501 caaagcgaag caatttcttc cgacacggtc catccagcga tcttctaacc ggactagcag
     4561 cacgacaagt tcctcctccg caacacacaa tgactggagg gccacggagc atttcgccga
     4621 ccgggacgaa tccttatgcg tcgttaccga cttgtcggac gcgacccagc gggacggaaa
     4681 tcaggcttcg ccgtccacct ccatggttct catcatacgc gccttatact cagccaccgc
     4741 gtggataaac ccagccgcgc cgagccggga ggctcggtaa gcttgcgatt gcttttcttg
     4801 taacta
//
LOCUS       DAORPM010000186         4769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5250_length_4769_cov_8.226559, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000186 DAORPM010000000
VERSION     DAORPM010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5250_length_4769_cov_8.226559"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..738
                     /locus_tag="PLD53_09070"
     CDS             61..738
                     /locus_tag="PLD53_09070"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRC61106.1"
                     /translation="MNNFDSFFWLIFVGIGVYFVFNVVRRGSLKGAIFNATIAQTVGE
                     VEGSGPSMMRTRVKVHILEKDAQKLVGLEFVATSVASYDMMPLTLSVTEAKKLVAVLE
                     QTIARTERNNRDGPRLISVKGSFAKHSRLSKPISGRSRMLGTVISAPEVTHVSKHGLW
                     LLLADEEILLPFEQFPWFRKATIDQISHVVAPTPEHLYWPELDIDLSVESIRHPETFP
                     LVSRSSG"
     gene            824..1468
                     /locus_tag="PLD53_09075"
     CDS             824..1468
                     /locus_tag="PLD53_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRC61107.1"
                     /translation="MTDLFPPWPLFSAFLLASLVLAVTPGPSVIYIVTRSLIQGRRSG
                     VVSVAGVALGNLGNAFAASVGLAALFAVSSLAFSVVKYAGALYLVYLGVRMLRSSLVE
                     NSAAVPAAAPHPRIFRDGFVVALLNPKTTIFFVAFLPQFLNPDAPPVFQSMALGFLFV
                     AIAAVTDSGYALAAGAAAPALRRSAVRRLGRRLGGGVFIGLGLFAAFAGSRGAK"
     gene            1640..2080
                     /locus_tag="PLD53_09080"
     CDS             1640..2080
                     /locus_tag="PLD53_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRC61108.1"
                     /translation="MTERTGRCLCGAVSFELAAEPLATRVCWCRDCQHLAANGSVNLL
                     VATDALTISGTLSEYTKTADSGNVVTRQFCPACGAHLFAKSSARPQFRVVRAGNLDEP
                     SSIHPDMNIWAESAPNWACLDPTLERVEQQPVPPKPSPASDQQT"
     gene            complement(2126..3073)
                     /locus_tag="PLD53_09085"
     CDS             complement(2126..3073)
                     /locus_tag="PLD53_09085"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRC61109.1"
                     /translation="MPTARDVLTPDALFMLQTIAAEGSFAAAARVANMVPSALTYRVR
                     QMEEALDVLLFDRSSRQARLTEAGEELLRESERLLADIDAVANRVRRVATGWESRFTI
                     AIDGIIDRTTVLELCEAFFSLNPPTRIRLRDETLAGTLGALSSGQADLALGVVMEAKS
                     APGLAREPMGGLHFIFAVAPHHPLATAAEPLSDDLIRRHRAVAVADSIQRGDDLTIGL
                     LPGQDVFTVPSLSAKLEAQIRGLGAGFLPACMAQTHINAGRLVEKSVERTEHKFQVSY
                     AWRKCDRTERGRALQWWLGQLKSPVTRAALLGQRPGALP"
     gene            3177..3878
                     /locus_tag="PLD53_09090"
     CDS             3177..3878
                     /locus_tag="PLD53_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRC61110.1"
                     /translation="MLNLIKSAERGHANHGWLVSKHSFSFADYYDPARMGWGNLRVIN
                     EDHIEPGTGFGKHAHRDMEIVTYVLSGELAHEDSMGNVKTIPPGDVQRMSAGRGVTHS
                     EFNHAKSQITHLLQIWIQPAVTGIAPGYEQKRFADAEKRGVLRLVASGDGAEGSVRIN
                     ADAALYAGLFDGAESAELTLDPKRKAYVHLIRGSLDVNGTQLAAGDAAALEGEARIAL
                     SQGHDAEVLVFDLAP"
     gene            3934..4488
                     /locus_tag="PLD53_09095"
     CDS             3934..4488
                     /locus_tag="PLD53_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRC61111.1"
                     /translation="MANIKVVFHSGYGHTQRMAEAVADGAGATLIAIDKEGNLSEADW
                     AALAAADAIILGSPTYMGSVSWQFKKFADASSKPWFTQQWKDKLFAGFTNSATMNGDK
                     LSTLHYMFTLAMQHSGVWVGTGMMPSNAKAAQRNDINYVGSFSGAMAQSPSDSSPAEM
                     LPGDLETARLFGKRVAETAARLKG"
     gene            4489..4641
                     /locus_tag="PLD53_09100"
     CDS             4489..4641
                     /locus_tag="PLD53_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61112.1"
                     /translation="MTRPAPADAHPAVFAGIVAFCEHEEYFALSGNDEGGHTRRDSCL
                     ANVLHA"
BASE COUNT          938 a         1461 c         1404 g          966 t
ORIGIN      
        1 cgataaagcc gcgcagcgcc ggttagctct actacaaggg catcttgatc ccttgcgcct
       61 atgaacaatt ttgactcttt cttttggctg atattcgtcg gtattggcgt ctatttcgtc
      121 ttcaacgtcg taaggcgtgg cagccttaag ggggcaattt tcaatgccac tattgcgcaa
      181 actgtcggtg aagtcgaagg ttctggccca tcaatgatga gaacccgggt caaggttcac
      241 attcttgaaa aggacgcaca aaaactggtt ggcctcgaat ttgtcgcaac cagcgtcgcc
      301 agctacgaca tgatgcctct cactctttcg gttaccgaag ccaagaagct tgtcgcggtt
      361 ctcgagcaaa ccatcgcaag aacggaacgc aataataggg acggaccacg attgatttct
      421 gtcaaaggca gcttcgcgaa gcacagtcgg ttatcgaaac ccatctcggg gagatcaaga
      481 atgcttggca cagtcatttc ggcgcctgag gttactcacg tttctaagca cggcttgtgg
      541 ttactgctgg ccgatgaaga aatccttctg ccatttgagc aattcccatg gtttcgtaag
      601 gccactatcg accagatttc tcatgtcgta gcgcctactc ctgagcatct ctactggccg
      661 gagcttgata ttgatttgtc ggtagaatcc attcgccatc ctgaaacgtt tccgcttgtc
      721 tcgcgctcat ccggctagcc gcttgttcat tcaacgcgga ctggcgcgat agtaccgtgc
      781 cggccgctta attgagacga ttgacccgtt catggttgcc gcgatgaccg atctctttcc
      841 accttggcct ctgttctccg cattcctgct tgcgagcctt gtcctcgccg tcacgcccgg
      901 gcctagcgtg atctacatcg ttacgcgcag ccttattcag gggcgccggt ctggcgtggt
      961 gtcggtggca ggtgtcgctc tcggcaacct cggcaacgcc tttgcggcct ccgtgggtct
     1021 tgccgcgctg ttcgcggttt cgtcacttgc tttttccgtg gtcaagtatg ccggcgcgct
     1081 ctaccttgtg tacctcggcg tgcgaatgct ccgttcctcc cttgtcgaga attcagccgc
     1141 cgttcccgct gctgcgccgc atccgcgcat ctttcgtgac ggcttcgtcg tagctctgct
     1201 caatccaaaa accacgatct tttttgtggc cttcctgccg cagttcctga accccgatgc
     1261 accgcccgtg tttcagagca tggcactggg ctttctgttc gttgcaatcg ccgcagtaac
     1321 cgacagcggc tacgctttgg ccgctggtgc ggcggcgcca gcgttgcgcc gtagcgctgt
     1381 tcggcgctta ggtcggcgtc tcggcggcgg agtcttcatc ggtcttggcc tcttcgctgc
     1441 gtttgcgggg tcgcgcggtg ccaaataggc ccgaccccgg ccttggcggc ggcgcacccc
     1501 tgaaaacagg acggttgata agaaatcgct gggtatcgac gctcaaccca gcggcgccgg
     1561 ggggcgcaat gcgataagtc cgctcaacgt cggctagctt ctgcgtttgg tcaaattgaa
     1621 tcgatggagg aacgaacaca tgactgaaag aaccgggcgc tgcctttgtg gcgcagtgag
     1681 cttcgaactt gccgccgaac ctctcgcaac gcgagtctgc tggtgccgcg attgccagca
     1741 tctcgcggca aacggatcgg tgaacctgct tgttgccacc gacgcactca caatctccgg
     1801 gacgctatcc gaatacacga aaacggcgga cagtggcaat gtggtgactc gccagttttg
     1861 tcccgcttgc ggtgcacacc tttttgccaa gtcgtccgcc cgcccgcagt tcagggttgt
     1921 ccgtgccggc aacctggatg aaccctcatc cattcacccg gacatgaata tctgggctga
     1981 aagtgcgccg aactgggcgt gccttgatcc aacgctggag cgagtcgagc aacagcccgt
     2041 gcctccgaaa ccgtcacccg cctcggatca gcagacctga tcgaatcacc cgccggcgag
     2101 tgcaaatgtt cgaccgcgct gcggactaag gcagcgcgcc ggggcgctgg cccagcaacg
     2161 cggcgcgggt gacagggctt ttcaactggc ccagccacca ctgcagggca cggccccttt
     2221 ctgtcctgtc gcattttcgc caggcataac tgacctgaaa cttgtgctcc gtgcgttcca
     2281 cggatttttc gaccaggcgt cctgcattga tatgggtttg cgccatgcag gcgggcagga
     2341 aaccggcgcc cagaccacga atctgggctt ccagtttggc cgacaggctg ggtaccgtaa
     2401 acacatcctg ccccggcaac aggccgatgg tgagatcgtc gccgcgctgt atcgaatcgg
     2461 ccaccgcgac ggcccggtgg cgacggatca aatcgtcgct aagcggctct gcggcagttg
     2521 cgagcgggtg gtgtggggcg acggcaaaaa taaagtgcag accgcccatc ggttcccgcg
     2581 ccaggccggg tgccgatttg gcttccatca ctacgcccag cgcgaggtcg gcctgacccg
     2641 atgacagggc acccagcgtc ccggccagcg tttcatcgcg cagccggatt cgggttggcg
     2701 gattcagcga aaagaaagcc tcgcataact caaggaccgt ggtccgatca atgatgccgt
     2761 cgatggcgat ggtgaaccgg gattcccagc ccgtggcgac gcgcctgacc cggttggcga
     2821 cggcatcgat gtcggccagc aggcgttcgc tctcacgcag cagttcctcg ccggcctcgg
     2881 tgaggcgggc ctgacgcgac gaacgatcga agaggagcac atccaaggcc tcttccatct
     2941 gccgtacgcg ataggtcagc gcgctcggca ccatgttggc gacgcgggcg gcagcggcaa
     3001 agctgccttc ggcagcaatg gtctgcagca tgaacaaggc gtcgggggtc aatacgtcgc
     3061 gggctgtcgg catcgagatc gcattcagaa aatttgaatg atgccatcaa aacggcaggc
     3121 tccaaagccc tccttttggg ctcaacctaa ccatcatcat cacaaggagg cccgacatgc
     3181 tgaacctgat caaatccgcc gaacgcggcc acgccaacca tggctggctg gtcagcaaac
     3241 attcgttttc ctttgccgac tactacgatc ctgcccgcat gggctggggt aatctgcgcg
     3301 tcatcaacga agaccacatc gagccgggca ccggcttcgg caagcacgcc catcgcgaca
     3361 tggagattgt cacctatgtg ctttccggcg agctggccca tgaggacagc atgggcaatg
     3421 tcaagaccat tccccctggc gatgtccagc gcatgagcgc cggcaggggc gtgactcaca
     3481 gcgaattcaa tcatgccaaa agccagatca cgcacctgct gcagatatgg atccagccag
     3541 ccgtcaccgg tatcgcgccg ggctacgaac agaaacgctt tgccgatgcg gaaaagcgcg
     3601 gcgtcctgcg cctggtggct tcaggggatg gcgccgaggg gtcggtacgg atcaacgccg
     3661 atgccgcgct gtatgccggc ctcttcgatg gtgccgagtc ggcggagcta acgctcgacc
     3721 cgaaacgcaa agcctacgtg catctgattc ggggcagcct cgatgtcaac ggcacgcagt
     3781 tggccgccgg cgacgcggca gcgcttgaag gcgaagcacg gattgcattg agccagggcc
     3841 acgatgccga ggtgctggtc ttcgatctgg caccttgaac accgttaaca caaacgcttt
     3901 ttcctcaaca cgaagataca aggagcattg aaaatggcaa acatcaaagt ggtattccat
     3961 tccggttacg gacacacgca gcgcatggcc gaagccgtgg ccgacggggc aggcgctacg
     4021 ctcatcgcga tcgacaagga aggcaacctg agcgaagccg actgggcggc gctggccgcc
     4081 gccgacgcca tcatcctggg cagcccgacc tacatgggca gcgtcagctg gcagttcaag
     4141 aagtttgccg atgcctcgtc gaaaccctgg ttcacgcagc aatggaagga caagctgttt
     4201 gccggcttca ccaacagcgc gacgatgaac ggcgacaagc tgtcgaccct gcactacatg
     4261 ttcaccctgg cgatgcagca ttcgggcgtc tgggttggta ccggcatgat gccgagcaat
     4321 gccaaggcgg cgcaacgcaa cgacatcaac tacgtcggct cattcagcgg cgccatggca
     4381 cagtcgccgt cggacagcag cccggcggag atgctgccgg gcgatctgga aaccgcgcgc
     4441 ctgttcggca agcgggttgc cgaaaccgcc gcccggctga aaggctgagt gacgcgaccg
     4501 gcacccgccg acgcgcaccc ggcagtgttc gccggaatcg tggcgttttg cgagcacgag
     4561 gagtactttg ccctatcggg caatgacgag gggggccaca ctcgccggga cagctgtctg
     4621 gcgaacgtgc tgcatgcctg attgggaacg gaccgactct tgatatcccg gagggttatc
     4681 tgcagaatgt ggcgatgaaa cgagcttttc cgcggcgtcg ctagacctca cgtccaccca
     4741 ttttttcatc gggatgcgca atgaccacc
//
LOCUS       DAORPM010000187         4752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5286_length_4752_cov_9.975729, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000187 DAORPM010000000
VERSION     DAORPM010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5286_length_4752_cov_9.975729"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PLD53_09105"
     CDS             <1..1247
                     /locus_tag="PLD53_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRC61113.1"
                     /translation="GVGDRIFVNGNAACGLGAVYGGATVAAWYPITPSTSVIEAFSGY
                     CRKFRTDPDNGMARYAVVQAEDELASIGMVIGAAWNGARAFTATSGPGISLMQEFFGL
                     AYFAEVPVVVFNVQRGGPSTGMPTRTQQSDLVSCAYASHGDTKHVLLFPEDPYECFKL
                     GAQAFDLADRLQTPVFVLLDLDIGMNDRLCQPFDWDDQRLYDRGKVMSAEDLEAGKNF
                     GRYLDVDGDGIPWRTIPGTHPSKGAFFSRGTTRNAYAKYSETGTDYVYNMERLRRKFE
                     TAKELLPAPVLQRASQPSRFGVIYYGSTGPAMDEAFALLERQGIHVNTLRVRAFPFAD
                     AVIEFINEHDCVFVVEQNESGQLRMLLINEGEIDPKRLVRVLHYDGTPITAGFISGSI
                     AEALSKHDRALAFASTRKQVAS"
     gene            1244..2305
                     /locus_tag="PLD53_09110"
     CDS             1244..2305
                     /locus_tag="PLD53_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRC61114.1"
                     /translation="MTYFIKPRLLRPDTPRNSLGYTRRDYEGAISTLCAGCGHDSISA
                     AVVQACFDLEIEPHRVAKLSGIGCSSKTPDYFLGNSHGFNTTHGRMPSVLTGAALANR
                     DLLYLGVSGDGDSASIGIGQFAHAMRRGVNMTYIVENNGVYGLTKGQFSATADKGSKS
                     KRGVVNSETPIDLVALALQLGASYVARSFSGDKEQLIPLIKGAIRHRGPSFIDVISPC
                     VTFNNHAGSTKSYDYVREHNDAVNRLDVILPRDRINTSYEPGSLRRVVQHDGSILNLR
                     KLDKDYDPSDRIGAMNHLQERQALGEIVTGLLYVDSSAEDLHRHLNTVEKPLNQLGDD
                     HLCPGSKALEALNASLRRA"
     gene            2405..3457
                     /locus_tag="PLD53_09115"
     CDS             2405..3457
                     /locus_tag="PLD53_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC61115.1"
                     /translation="MDRPGLLPSPGVSAALGAALLFGAGTPLAKLILSDVSPWLLAGL
                     FYLGSGIGLTAVRLVRRAAPVRLARNEARWLAGAILSGGIVGPVLLMYGLAGMPASGA
                     SLLLNAEAVFTVLLAWIVFREHIGRRIAFGMIAIIGGVLVLSWPEEVRFAGIWPVLSV
                     LGACLAWGLDNNLTRKVSLADATWIASVKGMVAGAINLALALFLGDRLPDLLILVASM
                     SVGFLAYGVSLTLFVIGLRQLGTARTGAYFSVAPFFGALIAVATGEPVTVRLAAAGML
                     MAVGVWLHLSERHDHEHQHAAIEHSHEHSHEEHHQHPHESFCAPGIRHAHRHRHESMV
                     HTHAHYPDAHHRDHSH"
     gene            complement(3507..4667)
                     /locus_tag="PLD53_09120"
     CDS             complement(3507..4667)
                     /locus_tag="PLD53_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRC61116.1"
                     /translation="MRISAHPFWLVGFRPFFTLACLSGLSLPLLWVLIHLGALPAPAT
                     PVGALQWHAHEMFFGFGWAVLGGFLLTATKNWVSIRGYHGTPLVVLVAAWLFERAGMW
                     FGADWPAALFALSNTLFLAAIVAMLLWTLVRHRKDDSYRDNYFFLLVLPAFLLAKQLM
                     LGGEHFQAGIAMAIGLFRMAFLVMFERTLTQFMKGVFQVSILRDPRLDGPIKLLALSL
                     VFVDLLPPWLGAAITLLLALLLIVRLFFWKPQLALRRLDIGIMYLGYLALVAQLLINV
                     VSAFTDIAWVGTVSIHVFTFGVMGMVIPAMLIRISKGHTGRKVVFDGLDKWVLRIMIL
                     GFIARVIVPQFAPAAYLHCIYLAAFCWFVCFSVLAWRYIPYLVQARVDGREH"
BASE COUNT          959 a         1386 c         1456 g          951 t
ORIGIN      
        1 acggcgtcgg cgacagaata ttcgtcaacg gaaacgctgc ctgcggtctc ggtgcggtct
       61 atggcggggc gacggttgcc gcgtggtacc cgatcacacc atcaacttcg gtgatcgagg
      121 ctttcagcgg ctattgccgc aaattccgca ccgatcccga caacggcatg gcacgctatg
      181 ccgtggtgca agccgaagac gaactggctt cgatcggcat ggtgatcggt gccgcctgga
      241 acggcgcccg tgcctttacc gccacctccg gccccggcat ctccctgatg caggagtttt
      301 tcgggctggc ctattttgcc gaggtgccgg ttgtggtttt caatgtgcag cgtggtggcc
      361 cctcgaccgg aatgccgacg cgcacccagc aatccgatct tgtttcctgc gcctatgctt
      421 cgcacggcga caccaagcat gttctgctgt ttccggaaga cccttacgag tgtttcaaac
      481 tcggcgccca ggcttttgat ctcgcggacc gtttgcagac accggttttc gttctgctcg
      541 atcttgacat cggaatgaat gaccgtctgt gccagccttt cgattgggat gatcagcgcc
      601 tttacgatcg tggcaaggtg atgagcgctg aagatctgga agccgggaag aattttggcc
      661 gctatctcga tgtggacggc gatggcatcc cttggcgcac cattcccggc acgcatccga
      721 gcaaaggcgc tttcttcagc cgcggcacca cacgcaacgc gtatgcaaaa tacagcgaga
      781 cgggaacgga ctatgtctac aacatggaac gtctgcggcg taaatttgag actgccaagg
      841 aacttctgcc ggcgcccgtt cttcaacgcg catcgcaacc aagccgcttc ggtgtcattt
      901 actatggttc gaccggtccc gcgatggacg aggcttttgc gctgctcgag agacagggca
      961 tccatgtcaa tacgctgcgc gtgcgcgcct tcccgtttgc cgacgcggta atcgagttca
     1021 tcaacgagca cgattgcgtc tttgtcgtcg aacagaacga gagtggacag ttgcgcatgc
     1081 tgctcatcaa cgaaggcgag atcgatccaa aacgcctggt tcgcgttctg cactacgacg
     1141 gtacgccgat taccgccgga ttcatttccg gcagcattgc cgaagccctc tcgaagcacg
     1201 acagggcttt ggcatttgcc tcgacccgca aacaggtggc gtcatgacct acttcatcaa
     1261 acccagactg ttgcggccag atacgcccag aaattcgctc ggttataccc ggcgcgatta
     1321 tgaaggcgcc atttccacgc tgtgcgccgg atgtggtcat gattcgatca gtgccgcggt
     1381 ggtgcaggcc tgcttcgatc ttgaaatcga gccgcaccgg gtagccaagc tttcaggcat
     1441 cggctgttcg tcaaagacgc cagattattt cctcggaaat tcacacggtt tcaatacgac
     1501 gcatggccgt atgccatcgg tactcactgg tgccgcactg gccaatcgcg atcttctcta
     1561 tttgggggtt tccggcgacg gtgattccgc ttccatcggc atcggccagt ttgcgcatgc
     1621 catgcgccgt ggcgtgaaca tgacctatat cgttgaaaac aacggcgtct atggtctgac
     1681 caaggggcag ttctcggcaa ccgccgacaa ggggtcgaag agcaagcgtg gcgtcgtgaa
     1741 tagcgagacg ccgatcgatc tggttgcgct cgcactgcaa ctgggtgcca gttatgttgc
     1801 gcgcagcttc tcaggcgaca aggagcaatt gattccgctg atcaagggcg ccatacgcca
     1861 tcgcgggccg tccttcatcg atgtgatcag cccttgcgtt acgttcaaca accatgccgg
     1921 ttcgaccaaa agttatgatt acgtgcgcga gcataacgat gccgtcaatc gtctggatgt
     1981 catcctgccg cgagaccgga tcaacacctc ctatgagcca ggctcgttgc gtcgggtcgt
     2041 tcaacatgac ggttcgatcc tgaatcttcg caagctggac aaggactacg atccgtcgga
     2101 ccggatcggt gccatgaacc atctacagga aagacaggct ctcggagaga ttgtcaccgg
     2161 cttgttgtac gtggacagca gtgccgagga tctgcatcgc catttgaaca cggtggaaaa
     2221 gccgctgaac cagctcggcg atgaccatct ttgtcccggc agcaaggcgc ttgaagcgct
     2281 gaacgcatcg ctcaggcgcg cctgacccag ttttggccgg ccgcgcgccg gcaagttcgg
     2341 agaatcaggt acgcacccca gatggccgcg acgttgaaag agctggacga aagaggttga
     2401 ggtaatggat cggccaggct tgttgccaag tcccggtgtg tcggcagcgc tcggcgcggc
     2461 tctgcttttt ggcgccggta cgccgctggc caagctcatc ctgagcgatg tcagtccctg
     2521 gctgctggcc ggcttgttct atcttggttc cggaattggc ttgaccgcag tccggctggt
     2581 gcgacgggcg gcgccggtcc gattggcgcg caacgaagct cgatggctgg cgggagccat
     2641 tctgtccggc ggaatcgttg gtcccgtcct gttgatgtac ggcttggccg gaatgcctgc
     2701 ctcgggcgct tcgctgcttc tgaatgcgga ggccgtgttc accgtgcttc tggcctggat
     2761 cgtttttcgg gaacacatcg gccggcgtat tgctttcggc atgatcgcca taattggcgg
     2821 agtgctcgtc ctgagttggc cggaagaggt caggtttgcc ggcatatggc ccgtgctgtc
     2881 agtactcgga gcgtgcctgg cctggggcct cgacaacaat ctgacgcgca aggtttcgct
     2941 tgccgacgcg acatggatcg catcggttaa agggatggtc gcgggtgcca tcaatctggc
     3001 gcttgcattg tttcttgggg accggttgcc ggatttgctc atcctggtcg cttcgatgag
     3061 cgtcgggttc ctggcctacg gtgtcagcct gacgctgttc gttatcgggc tgcggcagtt
     3121 gggaacagcc cgtaccgggg cctatttttc agttgcaccg tttttcggag cgctgatcgc
     3181 ggtggcgacg ggagagccgg tcacggtccg gctggcggct gccgggatgc tgatggccgt
     3241 gggcgtctgg ctgcacctgt ctgaacgcca tgatcatgaa caccagcacg ccgccatcga
     3301 gcattctcac gagcattccc acgaggaaca ccaccagcat ccccacgaaa gcttttgtgc
     3361 cccggggatc aggcacgcgc accggcatcg gcacgaatca atggtgcata cgcatgctca
     3421 ttaccctgac gcacaccatc gggaccacag tcactagatt cggtccggca aacgccgttt
     3481 tacgggagtt catgccttgt cgccgctcaa tgctccctgc cgtcaacacg tgcctgaaca
     3541 aggtagggaa tatagcgcca ggccagaact gaaaagcaga caaaccagca gaatgcggcg
     3601 agatatatgc agtgcaaata ggcggccggg gcgaattgtg gcacgataac gcgcgcaatg
     3661 aatccgagga tcattattct caacacccat ttgtcgaggc cgtcgaatac cacttttctg
     3721 cccgtatggc cttttgaaat ccggatcagc atggccggaa tgaccatgcc catgacgccg
     3781 aaggtaaaca cgtggatcga tacggtgcca acccaggcga tatcggtgaa cgcgctcacg
     3841 acgttgatca gcaactgggc gacaagggcc aggtagccaa gatacatgat gccgatgtca
     3901 agtcggcgca gggccagttg cggcttccag aaaaagagac ggacgatcag caggagggcg
     3961 agaagcaggg tgattgctgc acccagccag ggcggcaaca gatcaacaaa aaccaggctc
     4021 aaggcaagca gtttgatggg tccgtcgagc cgcgggtcgc gcaggatgga aacctggaag
     4081 acgcctttca tgaattgcgt cagcgtgcgc tcgaacatca ccaggaaagc catgcggaac
     4141 agcccgatag ccatggcgat gccggcctgg aagtgctcgc cgcccagcat cagctgcttg
     4201 gcgagcagga aggcggggag caccagcagg aagaagtaat tgtcgcgata gctgtcgtcc
     4261 ttgcgatgcc gtaccagggt ccacagcagc atggcgacga tggcggccag gaacagcgtg
     4321 ttggaaagcg cgaacagcgc cgccggccag tcggcgccga accacatgcc ggcgcgttcg
     4381 aacagccagg ccgcgaccag caccaccagc ggcgtgccgt gatagccgcg aatgcttacc
     4441 cagttcttgg tcgccgtcag caggaagccg ccgagcacgg cccagccgaa gccgaagaac
     4501 atctcgtggg cgtgccactg cagggcgccg acgggggtcg ccggcgccgg cagcgcgccg
     4561 aggtggatca gcacccagag cagcggcagc gaaagtcccg agaggcaggc cagggtgaaa
     4621 aacggccgga agccgaccag ccagaagggg tgcgcggaaa tcctcattgc gccagcgcgg
     4681 tcaccgactc gacacggttg cggccggcgc gcttggcctg gtacaacgcg gcatcggctc
     4741 gcgagatgat gt
//
LOCUS       DAORPM010000188         4734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5316_length_4734_cov_18.263518, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000188 DAORPM010000000
VERSION     DAORPM010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5316_length_4734_cov_18.263518"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PLD53_09125"
     CDS             complement(<1..963)
                     /locus_tag="PLD53_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRC61117.1"
                     /translation="MRFHFPIIIIDEDFRSENSSGLGIRALAAALEKEGLEVLGVTSY
                     GDLSSFAQQQSRASAFILSIDDEELAMEPEETLADLRAFVTEIRLRNADIPIFLHGET
                     RTSRHIPNDVLRELHGFIHMFEDTPEFMARNIKREARAYLDSLAPPFFRALVHYAADG
                     SYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVEELGQLLDHTGPVAASE
                     RNAARIFNADHLYFVTNGTSTSNKIVWHSTVAPGDIVVVDRNCHKSILHAIMMTGAIP
                     VFLMPTRNNFGIIGPIPKSEFAWANIQKKIAANPFITDKTAKPRV"
     gene            complement(1048..1614)
                     /gene="dcd"
                     /locus_tag="PLD53_09130"
     CDS             complement(1048..1614)
                     /gene="dcd"
                     /locus_tag="PLD53_09130"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRC61118.1"
                     /translation="MAIKSDKWIRRMAAEHGMIEPFSPELVREQNGQKIVSYGTSSYG
                     YDIRCAREFKVFTNINSTVVDPKDFDPKSFVEIESDVCIIPPNSFALARTMEYFRIPR
                     SVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVLFF
                     ESDEICETSYKDRGGKYQGQVGVTLPKI"
     gene            complement(1687..2778)
                     /gene="apbC"
                     /locus_tag="PLD53_09135"
     CDS             complement(1687..2778)
                     /gene="apbC"
                     /locus_tag="PLD53_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HRC61119.1"
                     /translation="MSLSEQQIKTALTAAVDPNTGKDFVTGKAVKNIKLDGNDVSFDI
                     ELGYPAKTQIDIIRKQVIAAVRTLPGIGNVSANVYVKIISHAVQLGVKLLPGVKNIVA
                     IASGKGGVGKSTTAVNLALALAQEGATVGILDADIYGPSQPQMLGLAGQQPESRDGTN
                     MEPLEAYGVQAMSIGFMVDVETPMVWRGPMVAQALDQLITQTNWRDVDYLIVDMPPGT
                     GDVQLSLAQKVPVTGAVIVTTPQDIALIDARKGLKMFEKVNIPILGIVENMSIHICSK
                     CGHEEHIFGSGGGERMGKDYDVEFLGSLPLELAIREMADGGKPTVVGAPDSRTAEIYR
                     GIARRVAVKIAEKAKDMTSKFPNIVVQNT"
     gene            complement(2854..3540)
                     /locus_tag="PLD53_09140"
     CDS             complement(2854..3540)
                     /locus_tag="PLD53_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC61120.1"
                     /translation="MNFAVVEDSRSQAEVLKALLKSEGHQVEVFAEGLACLEALKSRS
                     FDFFIIDWTLPDIGGDEVLKYIRENCGWDVPVVFCTASTTEENAADILRLGADDFIPK
                     PIRYMEFMARIESLLRRRQGARPSSLRFGNIEVDLAGRRVKLAGIDVDLTQREFDLAV
                     VFLSNVGRVLPREELLASVWVRDTEVDTRTVDTHASRLRKKLGLGGESGLMLSSVYGQ
                     GYRLDTVQQG"
     gene            3732..>4734
                     /gene="metG"
                     /locus_tag="PLD53_09145"
     CDS             3732..>4734
                     /gene="metG"
                     /locus_tag="PLD53_09145"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRC61121.1"
                     /translation="MSRKILVTSALPYANGAIHLGHLVEYIQTDIWVRYQKMVGNECW
                     YVCSDDTHGTPIMLRAEKEGITPEQLIARVHGEHSRDFSGFHVGFDNYYTTHSDETRD
                     CANDIYLKLRAAGLIETRTIEQYYDPVKQLFLPDRFIKGECPKCGAKDQYGDNCESCG
                     AAYAPTDLKDPYSAISGAKPELRTSEHYFFKLSDPRCESFLRQYTSRDNGVLQNEAAN
                     KMQEWLGAPGENKLTDWDISRDAPYFGFEIPDAPGKYFYVWLDAPIGYMGSFKNLCSK
                     NGLDFNEYFKPDSTTELYHFIGKDILYFHALFWPAELAHAGYRTPTKIFAHGFLTVDG
                     "
BASE COUNT         1072 a         1404 c         1269 g          989 t
ORIGIN      
        1 cacccgcggc ttggctgttt tgtcggtaat aaaggggttg gcagcgatct ttttctggat
       61 gtttgcccag gcaaactcgc tcttcgggat tggcccgatg atgccaaaat tgttccgcgt
      121 cggcatcaag aagaccggaa tcgccccggt catcatgatg gcgtgcagga tcgacttgtg
      181 gcagttgcgg tcgaccacaa cgatgtcgcc cggcgcgacc gtcgagtgcc agacgatctt
      241 gttcgaggtc gaagtgccgt tggtgacgaa atagaggtga tcggcattga aaatgcgggc
      301 cgcgttgcgc tcggaggcgg caaccgggcc ggtatggtcg agcaactggc cgagttcttc
      361 aacggcattg caaacgtcgg cacgcagcat gttttcgccg aaaaactgat ggaacatctg
      421 gccgaccggc gacttcagaa aggcaacgcc gcccgagtga cccgggcaat gccaggagta
      481 ggaaccgtca gccgcatagt gcaccagcgc ccggaaaaac ggcggcgcca gcgaatcaag
      541 gtaggcacgg gcttcgcgct tgatattgcg tgccatgaac tccggcgtgt cttcaaacat
      601 gtgaatgaag ccgtgcaact cacgcaacac gtcattcgga atatggcgcg aggtccgggt
      661 ctcgccatgc aggaagatcg ggatgtccgc attgcgcaga cggatttccg tcacgaaggc
      721 gcgcaggtca gccagcgttt cttccggttc catcgccagt tcttcatcgt caatcgacaa
      781 aatgaaagcg ctggcccgcg actgttgctg cgcaaatgag ctcaggtcac cgtaactggt
      841 cacgccgagc acttccagac cttctttttc cagcgccgcg gccagggcgc gaataccaag
      901 accggagcta ttctccgagc gaaagtcttc atcgatgatg atgatgggga agtggaagcg
      961 catgttgaat ccttgaaaag cggcgacccg ccgcagcggg tcacagaata agacgttttt
     1021 gttacagctt tgcgaaaaga cgcagggtca gatcttgggc agcgtcacac ccacctggcc
     1081 ctggtatttg ccgccgcgat ccttgtacga ggtttcacag atctcgtcgg attcaaagaa
     1141 aagcacctgg gcgcaacctt cgcccgcgta aatcttggcc ggcaacggcg tggtattgga
     1201 gaattccagc gtcacgtagc cttcccactc gggttcaaac ggcgtgacgt tgacgatgat
     1261 gccgcagcgg gcataggtgc tcttgccgag gcaaatggtc agcaccgagc gcggaatgcg
     1321 aaaatattcc atggtccggg ccagcgcgaa ggaattaggc gggatgatgc agacatcgga
     1381 ttcgatttca acgaaggatt tcggatcaaa atccttgggg tcgaccaccg tcgagttgat
     1441 attggtgaag accttgaatt cgcgggcaca gcggatgtcg tagccgtagc tcgaggtgcc
     1501 gtaggaaaca atcttctggc cattctgttc gcgcaccagt tccggtgaaa acggctcgat
     1561 catgccgtgc tctgccgcca tgcggcgtat ccatttatct gatttgatgg ccattttttc
     1621 ttatccgcgc tgcaaaaaac aaaggcggga ttttacccgc ctttgtgtag ggatttggcg
     1681 aaaattttag gtgttctgaa ccacgatatt cgggaatttc gaggtcatat ccttggcttt
     1741 ttcagcgatt ttgaccgcga cgcggcgggc aataccgcga taaatctcgg ctgtccggga
     1801 atcaggcgcg ccgacaacgg tcggcttgcc accatcggcc atctcgcgaa tggccagttc
     1861 aagcggcagg ctgccaagga attcgacgtc gtaatccttg cccatccgct caccgccgcc
     1921 actaccaaag atatgctctt cgtgaccgca tttcgagcaa atatggatgc tcatgttttc
     1981 aacgatgccg agaatcggga tgttgacttt ctcaaacatt ttcaagccct tgcgcgcgtc
     2041 gatcagcgcg atgtcctgcg gcgtggtaac aatgactgcg ccggtcaccg gcactttctg
     2101 cgccagcgat agctgaacgt cgcccgtacc cggcggcata tcgacaatca gataatcgac
     2161 atcgcgccag ttggtctggg taatcagttg atcaagcgcc tgcgccacca tcgggccgcg
     2221 ccagaccatc ggcgtttcga catcgaccat gaagccgatc gacattgcct gcacaccata
     2281 ggcctccagc ggctccatgt tggtgccgtc acgcgactcc ggttgttgtc cagccaggcc
     2341 aagcatttgc ggttgcgacg ggccgtagat gtcggcatca agaataccga ctgtcgcgcc
     2401 ctcctgcgcc agcgccagcg cgaggttgac cgcggtcgtg ctcttgccga cgccgccttt
     2461 gccggaagca atcgccacga tattcttgac gcccggtaaa agcttgacgc ccagctgaac
     2521 cgcgtgcgaa atgatcttta cataaacatt ggccgacaca ttgccgatac ccggcagcgt
     2581 gcgcacagcg gcaataacct gcttgcgaat aatgtcgatc tgggtttttg ccgggtagcc
     2641 aagctcgata tcgaaagaga catcgttacc gtcgagcttg atatttttca ccgctttgcc
     2701 ggtgacgaaa tccttgcctg tattggggtc gaccgcagca gtgagcgcgg tcttgatctg
     2761 ctgttctgaa agactcatgg aaactccagt tggggggaca gtcgggaata cccgagcaat
     2821 tctgtctagt atagccgaaa ctggaaaatc aaatcatccc tgctgaacgg tgtccaggcg
     2881 gtagccctga ccatagactg aactcagcat cagaccgctc tcgccaccca atccgagctt
     2941 cttgcgcagg cgactggcgt gggtatcaac cgtgcgggta tcgacttccg tatcgcgcac
     3001 ccagacgctg gccaacaact cttcgcgcgg caaaacccga ccgacattgc tcaagaaaac
     3061 gacagcgaga tcaaattcac gctgcgtcag gtcgacatcg atccctgcca gcttgactct
     3121 gcgtccagcc agatccacct caatattgcc gaaacgcagc gagctcggac gcgcgccctg
     3181 acggcgccgc aacagggact caatgcgcgc catgaactcc atatagcgaa ttggcttggg
     3241 aataaagtca tcggcaccca gccgcaggat atccgccgcg ttttcttccg tcgtactcgc
     3301 cgtgcaaaaa accaccggca catcccagcc acagttttcg cggatgtatt tgaggacttc
     3361 atcgccaccg atatcgggca acgtccagtc aatgatgaag aaatcaaagc tgcgggattt
     3421 caatgcctcc aggcaagcca gcccctcggc aaacacctca acctggtggc cttcactctt
     3481 caacagcgct ttgagcactt cggcctgact gcggctatct tctacaacgg caaaattcat
     3541 ggcacctccc aatcagggaa tattctgaag ggatcaggga accaagacaa taggcattaa
     3601 agccacatcc atttgtagac acaattgttt gcagttgtac acgttggcat aaaccgccgt
     3661 attgcccgaa aagacgtatc gcgctggcac gcggtaaaat tgcgctttat ttctcagcac
     3721 cctggcccct catgtcgcgc aaaattctcg tcacctccgc cctgccctat gccaatggcg
     3781 ccattcacct cggccacctg gttgaatata tccagaccga catctgggtg cgctaccaaa
     3841 agatggtcgg caatgaatgc tggtacgttt gttccgacga cacccacggc acgccaatca
     3901 tgctacgtgc cgagaaagaa gggatcacgc cggaacaact gatcgcccgc gtccatggcg
     3961 aacactcccg cgacttttcc ggctttcatg tcgggtttga caactattac acgacccact
     4021 ccgacgagac gcgcgactgc gccaacgata tttacctcaa actgcgcgcc gccggcctga
     4081 ttgaaacgcg cacgattgaa cagtattacg acccggtcaa acaacttttt ctgcccgacc
     4141 gcttcatcaa gggcgagtgc ccgaagtgtg gcgccaagga ccagtatggc gacaactgcg
     4201 aatcctgcgg agccgcttac gcgccgaccg acctcaagga tccgtattcg gccatctccg
     4261 gcgccaagcc ggaactgcgg acatccgaac actacttctt caagctatcc gacccgcgct
     4321 gcgagtcctt cctgcgccaa tacaccagtc gcgacaacgg cgtgctgcag aacgaagccg
     4381 ccaacaagat gcaggaatgg ctgggcgcac ccggcgaaaa caagctgacc gactgggata
     4441 tttcccgcga cgcgccttac ttcggctttg aaatccccga tgcgccgggc aagtacttct
     4501 acgtctggct cgacgcaccg atcggctaca tgggctcatt caagaacctg tgcagcaaaa
     4561 acggtctcga tttcaacgag tatttcaagc cggactcgac caccgagctt taccacttca
     4621 tcggcaagga catcctctac ttccacgccc tgttctggcc ggctgaactg gcccacgctg
     4681 gctaccggac accgaccaaa atcttcgccc acggctttct tacggtcgac ggcg
//
LOCUS       DAORPM010000189         4725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5332_length_4725_cov_10.444540, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000189 DAORPM010000000
VERSION     DAORPM010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5332_length_4725_cov_10.444540"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1467)
                     /gene="corA"
                     /locus_tag="PLD53_09150"
     CDS             complement(166..1467)
                     /gene="corA"
                     /locus_tag="PLD53_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRC61122.1"
                     /translation="MSEDKSILDHLANVKELLAKHKLVEELVHRQDMPRHDLVEEVVH
                     KQHLAGMRTLLERIPLAEIAQIIEALEPEDRLLVWEEVREERGEPILEILSDEVRQEL
                     IGDSHHRNEKTTVSAFELRNGRLSQINVNTPYDLKCLKPIWVDLVAPTPEIRAWVGRF
                     FDVQLPNPSNLTDLEASARFYIEDNGEIHLHSDFLLDTKEGSRNVAVALILHRDVLFT
                     VRTEELPVFRLQRLRARTQPGYVTDGKDVLLDLYAADAEYSADALEDVYAALEEVGKH
                     VLGTEITDKEAAMILADIAREEDLNGRIRRNVHDTRRAVSFLTRGKFLGPDQMEDARQ
                     ILRDIESLDGHTSFLFGKINFLMDATVGFININQNKIIKIFSVASVAMLPPTLIASIY
                     GMNFRWLPELQWEYGYPFSMTLMFVSVVVPFWFFRKKGWLR"
     gene            complement(1686..2393)
                     /gene="mtgA"
                     /locus_tag="PLD53_09155"
     CDS             complement(1686..2393)
                     /gene="mtgA"
                     /locus_tag="PLD53_09155"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRC61123.1"
                     /translation="MKSAAQLLSLSIKSLLLGLFGLLLLYQFWLFCWVLWWNWINPDT
                     TRFMAIRLAELQIKDPRAQLKKQWVGYGRISINLKRAIIVSEDDLFIHHEGFDWGGIQ
                     KAMEKNQRRGKIVAGGSTISQQLAKNLFLTPSKTPWRKAEEAIITLMIESVWSKQRIF
                     EVYLNVIEWGNGVFGAEAAARHYYGISAAQLGPEQAARLAGMVPSPRFYDRNRNAPGL
                     ANKAAIILARMPSAQVP"
     gene            complement(2390..3211)
                     /gene="aroE"
                     /locus_tag="PLD53_09160"
     CDS             complement(2390..3211)
                     /gene="aroE"
                     /locus_tag="PLD53_09160"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRC61124.1"
                     /translation="MTDHYCVFGNPIGHSKSPLIHMAFARQTDQDIQYTARLVPHDGF
                     ALAVHSFIKAGGRGANITVPFKEEAFRLVPYRTPRAELAGAVNTLVVTGSGIVGDNTD
                     GAGLLHDITLNLGYPVDGKRVLLLGAGGAARGVIGPLLDAQPEILVIANRTVSKAQAL
                     ARHFARLGKVSSSTYSELAGQSFDIIINATSASLGGEMPPLPAGLFSPYSLAYEMMYG
                     RGDTPFITFSKGQGAARFADGIGMLVEQAAEAFFIWRGVRPQSAPVIDMLRNADV"
     gene            complement(3216..4100)
                     /locus_tag="PLD53_09165"
     CDS             complement(3216..4100)
                     /locus_tag="PLD53_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRC61125.1"
                     /translation="MSRAVRQTRVSSSERNLWWAIGISMFLHAAILSLHFKFPDASRA
                     FQDKALDIILVNKKSARRPSDAQALAQANLDGGGNTDENRRVKTPLPPSEKQQSGSEL
                     EQAQKRVQALESLQERLLAKSRSKTAAPPKTENEAQPEPVQGVSGRDLANRALEMARL
                     QGEIDRDTDQYNKRPRKTFVGTRADEYRFARYIEDWRLKVERVGTLNYPEAAKGKLYG
                     TLILSVTIKSDGEVDKVEINRSSGHQILDDAARRIVTMSGPYAAFPPDIRRDTDLLVI
                     TRNWSFTTRNQLETKAQR"
     gene            complement(4102..>4725)
                     /locus_tag="PLD53_09170"
     CDS             complement(4102..>4725)
                     /locus_tag="PLD53_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRC61126.1"
                     /translation="MRGSPLDKLVAELMIVANSTWGGVLAEKGIPAIYRAQSAGKVRM
                     TTSPQAHDGLGVAQYAWSSSPLRRYVDLLNQWQLVACLNGDTPHFGAKSDALFAAMRD
                     FELTYGAYAEFQRGMERYWCMRWLCQEDVRTIEATIRRDNLVKLDNLPLIQRVNSVPE
                     LKPGQRIRLSIESVDYLTLELGCRYVETLDLAEKNAEEIDDTLEVIN"
BASE COUNT          878 a         1386 c         1484 g          977 t
ORIGIN      
        1 cgccagaacc tcggcggcga gcgtggcacg ctccggatgg tcgggtggca acaacttggc
       61 tggcgagggc gtgatactca aggggacctc cgggataggt cgtgctcgtt atttggatca
      121 agcgaactgc gctacaccat gatctgtcgc tgctcggtat tgcggttagc gcagccagcc
      181 cttctttctg aaaaaccaga atggaacgac gacggaaacg aacatcagcg tcatcgagaa
      241 ggggtagcca tactcccatt gaagttcggg gagccacctg aagttcatgc cgtagatgct
      301 ggcgatcagg gtcggtggca gcatggcgac cgaggccacg gaaaatatct tgatgatctt
      361 gttctggttg atgttgatga aaccgaccgt ggcgtccatc aggaagttga tcttcccgaa
      421 caggaacgac gtgtgaccgt caagcgattc gatatcgcgc agaatctggc gggcatcctc
      481 catctgatcg gggccgagaa acttgccgcg ggtgaggaag gaaacagcgc ggcgggtgtc
      541 atggacgttg cggcgaatgc gcccgttcag gtcttcctcg cgagcgatgt cggccagaat
      601 catggccgcc tccttgtcgg tgatctccgt gcccagcaca tgcttgccga cttcctccag
      661 cgcggcatag acatcttcca gcgcatcggc cgaatactcc gcatcggcgg cgtaaagatc
      721 gagcagcaca tccttgccgt cggtcacgta acccggttgg gtacgtgcgc gcagacgctg
      781 caggcggaag accggcaact cttcggtacg cacggtgaat aaaacatccc ggtgcagaat
      841 cagggccacc gccacgttgc gcgatccctc cttggtatcg agcaggaaat ccgaatgcag
      901 atgaatttcc ccgttgtcct cgatgtagaa acgcgcgctt gcctcaaggt cggtcaggtt
      961 gcttggattc ggcagctgaa catcgaagaa acgccccacc caggcccgaa tctcgggagt
     1021 cggcgcgacc agatcgaccc agatcggctt caggcatttc aggtcgtaag gcgtattgac
     1081 gttgatctgg ctgagccgtc cgttgcgcaa ttcgaaggca ctgacggtcg tcttttcgtt
     1141 gcggtgatga ctgtcgccga tcagttcctg acgcacttcg tcggacagaa tttcgaggat
     1201 cggctcgccg cgctcctcgc gcacctcttc ccacaccaac aggcgatcct cgggctccag
     1261 agcctcgatg atctgggcaa tctcggccag gggaatgcgt tcgagcagcg tacgcattcc
     1321 ggccagatgc tgcttgtgta cgacctcctc gacgagatca tgacgcggca tatcctggcg
     1381 gtgaacgagt tcctcgacca gcttgtgctt ggcaagcaat tccttcacat tggccaggtg
     1441 atccaggatg ctcttgtctt cactcatcgg gcaggactcc ccctgtactg caacgcgtca
     1501 accagacggg aattctagca gcctaccgga cttgatgggc ttgggtttgc tcggaattct
     1561 ggcgacccgc aaatataagc aggaggcctc gctgtcttaa cgcctgagca cgggccggtc
     1621 gcggggtgat gcggggctgg cacgacactt cgaagcgccg cccgatacag gtaatgcgtc
     1681 tgctttcaag gcacctgcgc ggacggcatt cgcgccaaaa taatcgcagc cttgttggcc
     1741 aggccgggcg cgttgcggtt gcggtcgtag aagcgcggac tcggcaccat accggccagt
     1801 cgtgccgcct gctcggggcc aagttgcgcg gcgctgatgc cgtagtagtg ccgcgccgcc
     1861 gcttcggcgc cgaatacgcc gttgccccat tcgatgacgt tgagatagac ctcgaaaatg
     1921 cgctgcttgc tccagaccga ttcgatcatc agggtgatga tcgcttcctc ggccttgcgc
     1981 cagggggttt tcgacggtgt cagaaaaagg ttcttggcca gttgctggga aatggtcgag
     2041 ccgccggcaa cgatctttcc gcgccgctgg ttcttttcca tggccttctg gattccgccc
     2101 cagtcgaatc cttcatgatg gatgaagagg tcgtcttccg agacgatgat ggcgcgcttc
     2161 agattaatcg agattcttcc ataaccgacc cattgtttct tgagctgcgc cctgggatcc
     2221 ttgatctgca attcggcgag ccggattgcc atgaagcgcg tggtatcggg gttgatccaa
     2281 ttccaccaga gtacccagca aaacagccag aactggtaga gcaggagcag gccgaacagg
     2341 ccaagtagca gactctttat cgagagggaa aggagttgtg cggcagactt cacacgtcgg
     2401 cgttccgcag catgtcgatc accggtgcgc tttgcggtcg cacgccgcgc cagatgaaga
     2461 aggcctctgc cgcctgctcg acgagcatgc cgatgccatc ggcaaagcgc gccgcgccct
     2521 gccctttgct gaaggtgatg aacggcgtat cgccacggcc gtacatcatc tcgtacgcca
     2581 ggctgtacgg ggaaaagagg ccggcgggta gcggcggcat ctcgccgccc aggctcgccg
     2641 acgtggcgtt gatgatgatg tcgaacgatt gcccggccag ttcggaataa gtactactgg
     2701 aaactttgcc aaggcgggca aagtgccggg ccagcgcttg ggccttggac acggtgcggt
     2761 tggcgatgac cagtatttcc ggctgcgcgt ccagcagcgg cccgatgaca ccgcgcgccg
     2821 cgccgccggc cccgagcagc agcacgcgct tcccgtcgac cgggtagccc agattgagcg
     2881 tgatgtcgtg cagcaatccg gcgccgtcgg tgttgtcgcc gacaatgccg ctgccggtga
     2941 ccaccagcgt attgaccgcg ccggccagct cggcacgcgg cgtccggtaa ggcaccaggc
     3001 gaaaggcttc ttccttgaac ggcacggtga tgttggcgcc gcgcccgccg gccttgataa
     3061 aggagtgtac ggccagggca aagccgtcat gtggcacaag gcgagccgtg tattgaatat
     3121 cctgatcggt ctgccgggca aacgccatgt ggatgagcgg cgacttgctg tgaccgatcg
     3181 gattgccgaa tacgcagtag tggtctgtca tggggctagc gctgtgcttt ggtttctagc
     3241 tggttgcgag tcgtaaagct ccagttgcgg gtaatgacaa gcaggtccgt gtcgcgccgg
     3301 atgtctggtg gaaaggcggc ataagggccg gacatggtga cgatacggcg cgcggcgtcg
     3361 tcgaggattt ggtggcccga ggagcggttg atctcgacct tgtcgacttc gccgtcactc
     3421 ttgatggtta ccgacaggat cagcgtgcca tagagtttgc ccttggccgc ttccggataa
     3481 ttgagcgtcc ccacgcgctc gaccttcagg cgccagtctt cgatatatcg ggcaaagcgg
     3541 tattcgtcgg cgcgcgttcc gacgaacgtc ttgcgcggcc gtttgttgta ctgatccgtg
     3601 tccctgtcga tctcgccctg caggcgcgcc atctccagcg cacggttcgc cagatcgcgg
     3661 ccgcttaccc cctggaccgg ctcgggctgg gcctcgttct cggttttcgg gggggccgcc
     3721 gttttgctgc gggacttggc gagcaaacgt tcctgcaggg attcaagcgc ctgtacacgc
     3781 ttctgcgcct gttccagttc gctgccgctc tgttgctttt ccgagggcgg cagcggcgtc
     3841 ttgacgcgtc ggttttcgtc ggtgttgccg ccgccatcga ggttggcttg cgccagcgcc
     3901 tgggcatccg acggccgtcg cgccgatttc ttgttgacca gaatgatgtc cagcgccttg
     3961 tcctgaaagg cgcgcgaggc atcgggaaac ttgaagtgca gcgagagtat ggccgcgtgg
     4021 agaaacatcg agatgccgat cgcccaccaa aggttgcgct cgctggatga aaccctggtt
     4081 tgacggacag cacggctcac gtcagttgat cacctcaagc gtatcgtcga tttcctctgc
     4141 atttttctcg gccagatcga gcgtctcgac ataacggcag ccaagttcga gcgtcaggta
     4201 atcaaccgat tcgatggaca aacgaatgcg ctggcccggc ttcaattcgg gtaccgaatt
     4261 gacccgctgg atcaagggca ggttgtcaag tttgacgaga ttgtcgcgcc ggatggtggc
     4321 ctcgatagtg cgcacgtctt cctgacatag ccagcgcata caccagtagc gttccatacc
     4381 ccgctgaaat tcggcgtagg cgccgtaggt cagttcgaag tcgcgcatgg cagcgaacag
     4441 ggcgtcggac ttggcgccga agtggggcgt atcgccgttc aggcacgcca ccagttgcca
     4501 ctggttgagc aggtcgacgt agcggcgcag cggcgacgac gaccaggcgt attgcgcgac
     4561 acccagtccg tcgtgcgcct gtggcgaggt cgtcatgcgc accttgccgg cgctctgcgc
     4621 gcggtagatg gccggaatgc ccttttcggc aagcacaccg ccccaggtgc tgttggcgac
     4681 gatcatcagt tcggcgacca gtttgtcgag cgggctgccg cgcat
//
LOCUS       DAORPM010000190         4695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5389_length_4695_cov_13.061422, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000190 DAORPM010000000
VERSION     DAORPM010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5389_length_4695_cov_13.061422"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLD53_09175"
     CDS             complement(<1..283)
                     /locus_tag="PLD53_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61127.1"
                     /translation="MSSRIPASSGVDVQVLGQLLLMQSVVASLPDDAIIPFVLEGLSD
                     IPGVGLVEFHAGSSSPDNAPERFLLACGPSVLGELVFQVKYMGAFEPYVD"
     gene            complement(289..909)
                     /locus_tag="PLD53_09180"
     CDS             complement(289..909)
                     /locus_tag="PLD53_09180"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HRC61128.1"
                     /translation="MSHEPHTISLGFTKQIFPAGMHVCQIFNHDEERLKSLLEFLRSG
                     LVAGERVACFTDNLDRAHLARHLAKHSLSSDGLQESGAFSCTAASDVYFQGGLFDPDR
                     MIGLLRQFQEDSVTNGFPACRVIGEMSAEVQHIPGGSRLLEYESRVSLLLNEHPVTAV
                     CQYDANTFDGATLMKILKVHPMMVIRGAVVRNPCYIQPDEFLTRNH"
     gene            complement(1050..2291)
                     /gene="ccmI"
                     /locus_tag="PLD53_09185"
     CDS             complement(1050..2291)
                     /gene="ccmI"
                     /locus_tag="PLD53_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HRC61129.1"
                     /translation="MTSTTGFILASALLVLVVLGILLPPLWRTPKPAKTVNRGEANLD
                     IFRDQMRELERDHDEGILTDADLKQAKSELQRRLLDEVHPETVPQAKSGGRQTALALL
                     IALPLAATAGYLILGNPQALDPTQTQTQTQTQIGPQQIEAMLGKLVEKLKQNPDDTKG
                     WVMLARSYKVLGRFSESAEAYSHGGALVDADPSLLADYAEVLAQANGGSLAGQPDKLL
                     SRALEIDPNEPQALFLAGAAASERRDFSAVARYWERLLQQLEPGSEEAQSLELAVAKA
                     REIDAQSGGKPPGITTTGPNAIPAESISGVVTISSKIAAQAKPGDLLFVFARAEEGSR
                     MPLAVVRAQLADLPLKFRFDDSMALPGGKKISGFKTVSVEARVAKAGKAQSSSGDLFG
                     SIKGVKPGSKNIKLVIDQIQP"
     gene            complement(2288..2812)
                     /locus_tag="PLD53_09190"
     CDS             complement(2288..2812)
                     /locus_tag="PLD53_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRC61130.1"
                     /translation="MNASFSKEPHKGREYFSAGFYFALLCVLCVSVVNPVFSKEATPL
                     AEDEVAEKRMVAISSELRCLVCQNESLSGSHAELANDLRREIRTLIKDGKSDGEIMDF
                     MVSRYGDFVRYRPPLKGTTLLLWFGPALLLVGAIGSLFVYLRRRNKAIIDSPLSAEEQ
                     DQADSLLNIEEPPK"
     gene            complement(2809..3393)
                     /locus_tag="PLD53_09195"
     CDS             complement(2809..3393)
                     /locus_tag="PLD53_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HRC61131.1"
                     /translation="MNRFLWPLIGFVVLVILLAVGLNLNPRDVPSPLVGKPAPIFSLP
                     QVAAPEQTFSPKDMLGKVWLLNVWATWCVSCRQEHPVLVEMAKNKAITLVGLNYKEVS
                     GDGALDSDKLTADAEMKLALERSNAWLNQHGNPYTLSVLDLDGRVGINYGVYGVPETY
                     VIDKAGIIRMKHTGPISPDDFSGKILPLVAELNK"
     gene            complement(3403..>4695)
                     /locus_tag="PLD53_09200"
     CDS             complement(3403..>4695)
                     /locus_tag="PLD53_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HRC61132.1"
                     /translation="GWGGWWFWDPVENASFMPWLIGTALIHSLAVTEKRGSFKNWTVL
                     LAIAAFSLSLLGTFLVRSGVLTSVHAFATDPTRGIFILVFLVAVIGSSLALFAWRAPK
                     VGLGGRFGLLSRESLLLTNNVLLVVACASVLLGTLYPLLIDALGLGKLSVGPPYFDSV
                     FAPLMIPALFLMGVAPFARWKQASFGELARILRLAFFAAAAVGIVAPFLFGEWKPLVA
                     LGLLLAAWIVLSGSLNFAGRVQAARAGQPFVTAALKQPRSFFGMHTAHVGVAVFVVGV
                     TMVNGYQTEKDVKMEIGDTVSVGGYTFRFNGVRQEKGPNYMALVGDIDLFNNDKRVRK
                     MHPEKRNYVSGSMPMTEAAIDTGLLRDVYISLGEPIDKSRPDGAWAVRVYHKPFVDWI
                     WGGCVLMAVGGLFALTDRRYRIKSRAASSQLGALARRA"
BASE COUNT          994 a         1374 c         1423 g          904 t
ORIGIN      
        1 ggtcaacata gggttcaaag gcccccatgt acttcacttg gaagacaagt tcgccaagga
       61 cgctggggcc gcatgcaaga agaaagcgct cgggggcgtt atccgggctc gaagacccgg
      121 catggaattc aacgagtccc actcccggaa tatcggacag cccctcgaga acgaagggaa
      181 tgatcgcatc gtccggaagc gaggcaacga cactctgcat cagcaagagc tgacccagca
      241 cttgcacatc cacaccagaa cttgccggta tccgggaact catgagcgtc aatgattgcg
      301 tgtgaggaat tcgtccggtt ggatgtagca ggggttgcga accacggcac cacggatcac
      361 catcatcggg tggactttaa gaatcttcat gagcgttgcg ccatcgaagg tgttggcgtc
      421 gtactgacag acggcggtaa ccggatgttc gttcagcaac aggctgacgc gagattcgta
      481 ctccagcaga cgcgaaccac ccggtatgtg ctggacctcg gcagacatct cgccgatgac
      541 gcgacaggca ggaaagccgt tggtgaccga atcttcctgg aactgacgca acaaaccgat
      601 catccggtcc gggtcgaaga ggccgccctg aaaatagacg tcactcgcgg cggtgcatga
      661 gaaggctccg gattcctgga ggccatcgct cgacaggcta tgtttggcga ggtgcctggc
      721 caggtgcgct cggtcgagat tgtcagtgaa gcaggcgacc cgctctcccg cgacgagtcc
      781 actgcgcaga aattcgagca gcgatttcaa gcgctcttcg tcatggttga agatttggca
      841 gacgtgcatc ccggcgggga aaatttgctt ggtgaatccg agcgaaatcg tatgcggttc
      901 gtgtgacatg agcgacccct tcgcaaagcc tgactatcaa acttacgtct tgggatactg
      961 tgcctcgcag atgccgctcc ggtatagcca gcggacggga aatcgctttg tccgagcggc
     1021 atgcgaaggc tcagtctagc agagttggct cagggctgaa tctgatcaat caccagcttg
     1081 atgttcttgc tgccgggttt gacgcccttg atactgccaa agaggtcgcc gcttgagctt
     1141 tgcgcttttc cggctttggc cacgcgcgct tcgacgctga ctgtcttgaa cccggaaatc
     1201 ttcttcccgc ccggcaaggc cattgaatcg tcgaaacgga atttgagcgg caggtcggca
     1261 agctgtgcgc gcaccaccgc cagcggcatg cgcgaccctt cttcggctcg cgcaaaaacg
     1321 aagagcagat cgccgggttt ggcctgcgcg gcaatcttgc tgctgatcgt caccacaccg
     1381 ctgatgcttt ccgcagggat ggcattaggg ccggtcgtgg ttattcctgg cggcttaccg
     1441 ccgctctgtg cgtcgatttc gcgcgccttg gccactgcca attccagcga ctgggcctct
     1501 tccgagccag gttcgagttg ttgcagcaga cgctcccagt atcgggccac ggcggaaaaa
     1561 tccctgcgct ctgaggccgc cgcacccgcc aggaacagcg cctgcggttc gttcgggtca
     1621 atctccagcg cgcgggaaag caacttgtcg ggttggcccg ctaagctgcc accattggct
     1681 tgcgccaata cttcggcata atcggccagc aagctggggt cagcatcgac cagtgcgccg
     1741 ccgtgactgt aggcctcggc cgattcggaa aaacggccca ggaccttgta ggaacgggcg
     1801 agcatgaccc accctttggt gtcatccggg ttctgcttga gcttttcgac caatttgcca
     1861 agcatcgctt cgatctgctg tggcccgatt tgcgtctgcg tctgcgtttg cgtttgcgtc
     1921 ggatcgagcg cctgcggatt gccgaggatc aggtaaccgg ccgtcgcggc cagcggaagc
     1981 gcgatgagca aggccagcgc ggtctggcgt ccgccgctct tcgcctgtgg caccgtctcg
     2041 ggatgaacct cgtcgagcag acggcgctgc agttcgcttt ttgcctgttt cagatcagcg
     2101 tcggtgagaa ttccttcatc gtggtcgcgt tcgagttcgc gcatctggtc acgaaaaatg
     2161 tccagattgg cttcacctcg attgaccgtc tttgccggct tgggggtacg ccaaagcggc
     2221 ggcaacagaa tgcccaggac gacgaggacg agcagcgccg aggccaggat gaaaccagtc
     2281 gtggaagtca tttcggcggt tcttcgatat tgagcaggct gtcggcctga tcctgttctt
     2341 ccgcactgag cggcgagtct atgatggcct tgttacggcg gcgcagataa acaaagagtg
     2401 agccgatggc gccgacgagg agcagcgccg gaccaaacca caaaagcagg gttgttccct
     2461 tgagcggcgg acggtaacgc acgaagtcgc cgtagcggct gaccatgaaa tccatgatct
     2521 cgccgtcgct cttgccgtcc ttgatcagcg tacgaatctc gcgacgaagg tcgttcgcca
     2581 gttcggcgtg ggagccggac aaggattcgt tctggcagac cagacagcgc aattcagagg
     2641 aaatcgccac catgcgcttt tcggcgacct catcctcggc gagtggcgta gcttccttgg
     2701 aaaaaaccgg atttaccaca gagacacaga gaacacagag aagagcaaag tagaaaccag
     2761 ccgagaaata ttctctgccc ttatgtggct ccttggagaa agatgcgttc atttattcaa
     2821 ctccgcaacc agcggcagaa tcttgcctga aaaatcatca ggactgatcg gccccgtgtg
     2881 cttcatgcgg atgatgccgg ccttgtcgat gacgtaggtt tcgggtacgc catagacgcc
     2941 gtagttgatg ccaacgcggc cgtcgaggtc gagtactgaa agtgtgtagg gattgccatg
     3001 ctggttcagc caggcgttcg accgttccag tgccagcttc atttcggcgt cggcggtgag
     3061 cttgtccgaa tccagtgcgc cgtcaccgct cacttccttg tagttgagcc cgaccagcgt
     3121 tatcgctttg ttcttcgcca tttcgaccag taccggatgt tcctgccggc atgaaacgca
     3181 ccaggtcgcc cagacgttga gcagccacac cttgccgagc atgtccttcg gcgagaaggt
     3241 ctgttccggc gcggccactt gtggcagcga aaatatcggt gccggcttgc cgaccaatgg
     3301 cgacggcaca tcgcgcggat tgaggttgag cccgacagcg agcaggatca caagaacgac
     3361 aaagccgatc agcggccaga gaaagcggtt catgatttgt cctcaggcgc gccgggccag
     3421 cgcgccaagt tgactcgatg cggctcggga ctttatccga tagcgacggt cggtcaaagc
     3481 gaagaggccg ccgaccgcca tcagcacgca accgccccag atccagtcga caaatggttt
     3541 gtgatatacg cgaacggccc aggcgccgtc tggtcgggac ttgtcgatcg gttcgccgag
     3601 cgagatgtaa acgtcgcgta gcaggccggt atcgattgcc gcctcggtca tcggcatcga
     3661 tccggaaacg tagttgcgtt tctccgggtg cattttccga acgcgcttgt cgttgttgaa
     3721 caggtcgata tcaccgacta gcgccatgta gttgggcccc ttttcctgac ggaccccgtt
     3781 gaatctgaag gtgtaacccc ccacgctgac ggtatcgcca atttccattt tcacgtcttt
     3841 ttcggtttgg tagccattga ccatcgtcac cccgacgacg aagacggcaa cgccgacatg
     3901 ggccgtatgc atgccgaaaa agctgcgcgg ctgcttcaag gcggcggtca cgaagggctg
     3961 gccggcgcgc gctgcctgca cgcgcccggc gaaattgagt gagcccgaga gaacgatcca
     4021 cgcggcaagc agcaggccca gggccaccag cggcttccat tcgccgaaca ggaaaggcgc
     4081 cacgatgccg accgctgccg ccgcgaaaaa agccaggcgc aggatgcgcg ccagctcgcc
     4141 gaagctggcc tgcttccagc gtgcaaacgg agcgacgccc atcaggaata gcgccgggat
     4201 catcaacggt gcgaagacgg aatcaaagta gggcgggccg accgacagct tgcccaggcc
     4261 gagcgcgtcg atgagcagcg ggtaaagcgt gccgagcaaa acggaagcac aggcgacgac
     4321 cagcagaaca ttgttggtga gcagtagcga ttcacgtgaa agaaggccga atctgccgcc
     4381 cagaccgacc tttggcgctc gccaggcaaa gagtgcgagc gagctgccga tgactgcaac
     4441 cagaaagacg agaatgaata tgccgcgcgt cggatcggtg gcaaaggcat gcaccgaggt
     4501 cagcacgccg gaacgcacca ggaatgtacc gagcagcgag agcgagaatg ccgcaatggc
     4561 cagcagcacc gtccagttct tgaagctgcc gcgtttctcg gtcaccgcca gtgagtgaat
     4621 cagcgcggtg ccgatcagcc atggcatgaa cgaagcgttc tcgaccgggt cccagaacca
     4681 ccagccgccc cagcc
//
LOCUS       DAORPM010000191         4649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5462_length_4649_cov_10.202656, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000191 DAORPM010000000
VERSION     DAORPM010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5462_length_4649_cov_10.202656"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLD53_09205"
     CDS             complement(<1..593)
                     /locus_tag="PLD53_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HRC61133.1"
                     /translation="MHKSFTLRVLQSLNRRSWLALGFFLVVTLVAVPLLHLLVPPESA
                     FHVSTYAITLCGKIMCYAIVAVAMDLIWGYGGILSLGHGLFFALGGYAFGMYLMRQIG
                     RDGSYQSDLPDFMVFLDWKELPWYWSGSDNLLWVGFLVIAVPAIVAFIFGYFAFRSRI
                     KGVYFSIITQALTYAAMLLFFRNETGFGGNNGFTDFKR"
     gene            complement(602..2188)
                     /gene="urtB"
                     /locus_tag="PLD53_09210"
     CDS             complement(602..2188)
                     /gene="urtB"
                     /locus_tag="PLD53_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HRC61134.1"
                     /translation="MNKLFTLFTFCLLYACSQLAHAALDPALVRQLAAEDSSEKVSAI
                     RQITQNADNDAVRVLQAMADDQLFLSADTVVVFDGENAFDAASGEPIKTPKQPESIIV
                     NNRIRGELANALAALSLFNKDVSIRLASAQELQTRVSPDIVPVLALALSKETNGEVKA
                     LLKLAHAQANLGNADPAARLAAVRALSDASSPVVRSALLPLTDKAKEPDNRVRFAAIS
                     AVKSISGRLELTENLGRIFSGLSLGSILLLAALGLAITYGVMGVINMAHGELLMVGAY
                     SAYAVQSVFRSYFPDAIDWYLPAAIPVAFFAAALIGVVMERTVIRWLYGRALETLLAT
                     WGLSLVLIQAARVIFGAQNVEVSNPSWMSGGVELIPSLILPWNRIIIIGFALFVLLLV
                     WVLMNKTRLGMFVRAVTQNRSMAGCVGVPTARIDTMAFALGAGIAGLGGLALSQISNV
                     GPDMGSGYIVDSFMVVVVGGVGQLAGAVWAALGLGIFTKLLEGWAGAVIAKIAVLVFI
                     IIFIQKRPQGIFALKGRVVE"
     gene            complement(2403..3020)
                     /locus_tag="PLD53_09215"
     CDS             complement(2403..3020)
                     /locus_tag="PLD53_09215"
                     /inference="COORDINATES: protein motif:HMM:NF025850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRC61135.1"
                     /translation="MSKPRLIYFDFAGSRGEECRIALHLAGVDFDDVRIPIAEWPELK
                     PQTPFGSLPILEIAGKPPLAESNAILVYIGRQHGLHPKDALDAAYHEALMSYAEDLRH
                     NVGPTLRITDEQQKRSAREELARNFLPTWGTNVERQLGDQPFVGGDTLSVVDIKLYMI
                     VRWFVSGTVDHVPSTVFDHCPKLKRLFQQVGGHAGVKAWLERAAG"
     gene            complement(3301..3984)
                     /locus_tag="PLD53_09220"
     CDS             complement(3301..3984)
                     /locus_tag="PLD53_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRC61136.1"
                     /translation="MGKSTAIRKRSTRHNGATAESRVVRESVLNQARRTLILDAARSA
                     FFELGLDGASLREIAKRAGYTPGAIYSYFANLEEIYGALLGESLQRLNQVVEAAKGAG
                     KSSSAKAIDLLRAKAEAFYVFYRDNPKELDLGFYLFDGVKPRGLTPELNQQLNEQLMA
                     ALRPVQVQLEALGLSADESVIETSAVFAHIVGVLVVNNTGRIRLFKQNPDSLFERYMK
                     LLIQRLPTA"
     gene            4058..>4649
                     /locus_tag="PLD53_09225"
     CDS             4058..>4649
                     /locus_tag="PLD53_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRC61137.1"
                     /translation="MSATSASGLMDGKEFRESLRKYKPTVYVDGQLIESVVDAPSLQP
                     GINALAVTYDFAHDPALAPLMLATQTASGKTVNRMLHVDESAGDLLNKLEAVRVLCQE
                     TGCAQRYLAHDALNGILQAVAKIDDAKGSTEHSAKFTAYLQKVQDEDLTLGIAMTDAK
                     GDRSKRPNQQLNQDVYVHIVERNAKGIVITGTKAIVT"
BASE COUNT         1093 a         1324 c         1368 g          864 t
ORIGIN      
        1 cgcttgaagt cggtgaagcc gttgttgccg ccgaaaccgg tttcgttgcg gaagaagagc
       61 agcatggcgg catacgtcag cgcctgcgtg atgatcgaaa aatagacacc cttgatgcgc
      121 gaacggaagg cgaaatagcc gaaaatgaag gcaacaatgg ccggcacggc aatgaccagg
      181 aagcccaccc acaagagatt gtcgctgccg ctccagtacc agggcagttc cttccagtcg
      241 aggaacacca tgaaatccgg caggtcgctc tggtagctgc catcgcggcc gatctggcgc
      301 atcaggtaca tgccgaaagc gtaacccccg agagcgaaga agaggccgtg gcccaacgac
      361 agaatgccgc catagcccca gatcaggtcc atggcgacgg ccacaatggc gtagcacata
      421 atcttgccgc acaaggtgat agcgtaggtc gacacgtgga aagcgctctc gggcggaacc
      481 agaagatgga gcagcggcac cgcgaccagc gtgacgacaa gaaagaatcc cagcgccagc
      541 cagcttctgc gattcagcga ctggagaacg cgcagggtga atgacttgtg catgatggcg
      601 ctcattcgac gacacggccc ttgagggcga agatgccctg cggtcgcttt tgaataaaga
      661 taataatgaa aacgagaaca gcaatcttgg cgatcactgc acctgcccag ccttcaagga
      721 gctttgtgaa aatccccagg cccagtgccg cccataccgc gccggccaac tgcccgacgc
      781 cgccgacgac gacgaccatg aaggaatcga cgatgtagcc actgcccatg tccggcccga
      841 cgttggaaat ctgtgacaac gcgaggccgc caaggccggc gatgccggcg ccgagagcga
      901 aggccatggt gtcgatgcgt gccgtcggca cgccgacgca gccggccatg gatcggttct
      961 gcgtcacggc acgaacgaac atgccgagcc gcgtcttgtt catcagcacc cagaccagca
     1021 gcaacacgaa tagcgcgaaa ccgatgatga tgatgcggtt ccacggcaag atcaggctgg
     1081 gtatcagctc aacgccgccg gacatccagc tcgggttgga gacttcgacg ttctgcgcac
     1141 cgaaaatcac gcgcgcggcc tggatcagca ccagcgacag gccccaggtg gcaagcaggg
     1201 tttccagcgc acgaccgtac aaccagcgga tgacggtgcg ctccatgacg acgccgatca
     1261 gcgccgcggc gaagaaggcg acaggaatgg cggccggcag ataccagtcg atggcgtccg
     1321 gaaaatagct gcggaaaaca ctctgcacgg cataggcgga ataggcgccg accatcagca
     1381 gttcgccgtg cgccatgttg atgacgccca tcacgccgta agtgattgcc aggccgaggg
     1441 cggcaagcag caggatgctg ccgagcgaca gcccggaaaa tatccggccc agattctcgg
     1501 tcagttccag tcggccagag atggacttga cggcggaaat ggctgcgaaa cgcaccctgt
     1561 tgtccggttc cttcgctttg tcggtgagcg gcagaagcgc gctcctgacg actggcgacg
     1621 aggcgtccga gagtgccctg acggccgcca gtcgcgccgc cggatcggca ttgccaaggt
     1681 tggcttgagc gtgcgccagc ttgagcagcg ccttgacctc gccgttggtt tccttggaca
     1741 aagcgagggc tagcacagga acgatgtcgg gactgaccct cgtttgcagc tcctgcgcag
     1801 aagcaaggcg gatgctaacg tccttgttga aaagactgag cgccgccaac gcattggcca
     1861 gttcgccgcg aatccggttg ttcacgatga ttgactcggg ttgcttggga gtcttgatcg
     1921 gctcaccgct ggccgcgtca aaggcatttt caccatcgaa gacaacgacc gtatcagcac
     1981 tcaagaagag ttgatcgtca gccatcgcct ggagaacgcg cactgcatcg ttgtcggcat
     2041 tctgcgtaat ctgccggatg gcgcttacct tttcgctcga atcctcggcc gccaattggc
     2101 gaaccaaggc cggatcaagc gcggcatgcg ccaactggct gcaggcatag agcaggcaaa
     2161 aggtaaaaag ggtgaaaagc ttgttcatca aaaggaaact cacaattcgt acaggtctct
     2221 gacaggttaa ggaccacggc ggaatggagg aatacgttga attgcgtagg gactgaaaac
     2281 gacagctatg ctcattgggg agaacggctg gatctttgcg gaaggcgagg aaaacaagcg
     2341 ccgaggcgtt cctgtccgga cgcttaaaga ctgacgataa tcaccacgcc taccaaggaa
     2401 atctagccgg ccgcacgctc cagccaggcc ttcacgccag catgcccgcc gacctgctgg
     2461 aagagacgct tcagcttcgg gcagtggtcg aagacggtcg aaggcacatg gtcgaccgtc
     2521 cctgaaacaa accagcgcac aatcatatag agtttgatat cgacaacgct cagcgtatcg
     2581 ccgccaacga agggctgatc gccgagctga cgttcgacgt ttgtgcccca tgtcggcagg
     2641 aagttgcgtg cgagctcctc gcgtgcgctg cgcttctgtt gttcgtcggt aatacgcagc
     2701 gtcgggccga cgttgtggcg cagatcctcg gcgtagctca tcagcgcctc gtgatatgca
     2761 gcgtcaagag catcctttgg gtgcagtcca tgctgccggc cgatgtagac aagaattgcg
     2821 ttcgattcgg caagcggtgg cttgccggca atttcgagaa tcggcaagga gccgaacggg
     2881 gtctgcggtt taagttccgg ccattcggca atcgggatgc gcacgtcgtc gaaatcgacg
     2941 cccgcaaggt gcagggcaat gcggcattcc tcgccgcggc tgccggcaaa gtcaaagtaa
     3001 atgaggcgtg gcttggacat gcgatggttc cttcgtcaaa ttgtggaccg gacacgggcg
     3061 tgctcgagtc gaattgtaga ggggcattat cggtacacac gagcacgttg gcaatgatgc
     3121 cgcctagtac agggattgga cgttcaggcc gacaaggaaa tcgtacccac tcaatgtttc
     3181 agaaacgagt gacgagtctt cgaggtcgca aactcacccg ttcgacctac ttgagacatt
     3241 caacactgga ttggtccaga ggcagctaac cgttggtagc cgccaatcta cgtttaacgt
     3301 ctaagcagtg gggagtcgct gaatgagcaa cttcatatat cgctcgaaaa gcgagtccgg
     3361 gttctgcttg aataggcgga tacgtcccgt gttgttcacc accagaacac ccacaatatg
     3421 ggcaaacacc gcgctcgtct cgatgacaga ctcgtcggca ctcaaaccca aggcttccaa
     3481 ctggacctga accggccgta gtgcagccat caactgttcg ttcaactgct ggttgagttc
     3541 gggggtcagc cctcgaggct ttactccatc aaacaggtag aagcccaagt ccagttcctt
     3601 gggattgtcg cggtagaaca cgtaaaaggc ttcggctttg gcgcgaagca agtcgattgc
     3661 cttggcagag gatgacttgc cagccccctt ggcggcctcg accacttgat tgaggcgctg
     3721 caaggattcg ccgagcaggg ccccatagat ttcttccagg ttggcgaagt aactgtagat
     3781 ggcacccggc gtataacccg cccgcttggc gatttcccgc aggcttgcgc catccaggcc
     3841 cagctcaaaa aacgccgagc gcgccgcatc gagaatcaac gtccgccgtg cctgattgag
     3901 aacactttcc ctaaccacgc gagattcagc cgtggcgcca ttatgtcggg tgctgcgttt
     3961 ccggattgcg gttgactttc ccatagacca aattatacac tgcaatttcc gattcaacac
     4021 tgttcaacaa tataaacgct gtgcgagagg aatggttatg tctgctacga gtgccagtgg
     4081 cttgatggat ggtaaggagt ttcgggagtc gcttagaaag tacaaaccca ccgtctatgt
     4141 ggatggacaa ctgattgagt cagtcgttga tgcaccttcg cttcagcccg gaatcaacgc
     4201 gctggcggtc acctacgatt tcgcccatga ccccgccctc gccccgctga tgctagctac
     4261 gcagacggcc tccggcaaaa cggtcaaccg gatgctacat gtcgatgaat cggcgggtga
     4321 cctgctgaac aagttggaag ctgttcgtgt gctgtgtcaa gaaaccggct gtgctcagcg
     4381 ctatctcgca cacgatgctc tcaacggtat cttgcaggca gtggccaaaa tagacgatgc
     4441 aaagggttcg accgagcaca gcgccaagtt caccgcctat ctccagaagg ttcaggatga
     4501 agacctgacg ttgggtatcg ccatgaccga cgccaaggga gatcgtagca agcgtcccaa
     4561 ccagcaattg aaccaggacg tgtacgtcca tatcgtcgag cgcaacgcaa aaggtatcgt
     4621 catcaccggc accaaggcga ttgtgaccg
//
LOCUS       DAORPM010000192         4636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5481_length_4636_cov_16.669504, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000192 DAORPM010000000
VERSION     DAORPM010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5481_length_4636_cov_16.669504"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLD53_09230"
     CDS             complement(<1..440)
                     /locus_tag="PLD53_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC61138.1"
                     /translation="MTIATVHCDRTDPIATVTLSNPGKLNAMNATMWQELRHTMLRLA
                     EDADIRCVIVRGAGEVFAAGGDIEELREARATVDSALAYHESVGQVLQAIQQLPQPTI
                     AAIQGACAGAGLEIACSCDLRIAGQSARFGAPVMKLGFSMYPGEL"
     gene            983..2425
                     /locus_tag="PLD53_09235"
     CDS             983..2425
                     /locus_tag="PLD53_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRC61139.1"
                     /translation="MKLTLTHDEMNAYCSELHDSNAAFNIHYPADSFHRQPVHTVYGG
                     ANLFKAGFSAKLGEVALKTLETHAPTYSVFARALGMPRADTLPTDPTELANLTRALET
                     NPELVRETKQAAWLAYTVYNRVVKKLQTEPIEDNRIDFEDGYGNRPDEEEDAHAIAAA
                     DEVAKGMSENILSPFIGIRIKTFSDECKVRSIRTLDLFLTRLAEKTGSKLPNHFIVTL
                     PKVNTPTQVSTCVKVLEKLEAKLGFAKDSLKMEIMIETTQSIINHKGENTVPLLVAAA
                     GGRCRSAIFGTYDYTASCNITAAHQKHTHPACDFARHVMQVSLAGTGITISDGATTSM
                     PIGPHKAGKDDVLTSQQRYENRMVVHGAWKLHYDNIVHSLAHGYYQGWDLNPGQLPIR
                     YAAMDAFFLSGLQDASARLNAFLNKAAQATLVGNTFDDAATGQGLLNFFLRGMACGAI
                     TEQEVLATGITLDELRSRSFVRIVNNRTKK"
     gene            2459..>2659
                     /locus_tag="PLD53_09240"
     CDS             2459..>2659
                     /locus_tag="PLD53_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61140.1"
                     /translation="MTAVKSGMQGAVQHVGSADALIKKSLSWVKQQCLGGKGVSGEKL
                     DEHQMASFDLAWCAAEATAARFA"
     assembly_gap    2660..2759
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2760..4055
                     /locus_tag="PLD53_09245"
     CDS             <2760..4055
                     /locus_tag="PLD53_09245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC61141.1"
                     /translation="LSSFCQTQLDAGNLDALGKLVSTLDGASGAYLLGDDATMMQDSF
                     RKLATNVVMPLAEEIHREDRIIPPEILEPLTEMGCFGLSIPERYGGIQSDDHEDNMGM
                     IVVTEELSRGSLGGAGSLITRPEILSRSLLKGGTEAQREKWLPQVAMGQPLCAIAVTE
                     PDTGSDVAAMRLKATKCDGGWVLDGAKTWCTMAGKAGLLLVLARSNPDSSLGHKGLSM
                     FLVEKDSYEGHDFEYRQPQGGVLTGRAIATIGYRGMHSYEVFFDHVFVPDENLVGGPE
                     GEGKGFYFTMAGFAGGRIQTAARAIGVMQAAFEKAVSYSQERKAFGKPIGDFQLTRVK
                     LARMATLLTVSRQFTYAVGRLMDQGKGQMEASMVKLFTCRTAEWLTREAMQIHGGMGY
                     AEETSVSRYFLDARVLSIFEGAEETLALKVIARSLIDAV"
     gene            4099..>4636
                     /locus_tag="PLD53_09250"
     CDS             4099..>4636
                     /locus_tag="PLD53_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRC61142.1"
                     /translation="MASTQKKTDRPWLMRTYSGHSSAKASNTLYRTNLAKGQTGLSVA
                     FDLPTQTGYDADNPLAKGEVGKVGVPISNIEDMDQLFDQIPLDQMNTAMTINATAPWL
                     LSLYIGLAQRRGIDPKLLSGTTQNDIIKEYLSRGTYIYPPGPSMRLIADTINYTVKNV
                     PKWNPTNICSYHLQEAGAT"
BASE COUNT         1001 a         1228 c         1352 g          955 t
ORIGIN      
        1 agttcgccgg ggtacatgga aaacccgagc ttcattaccg gtgcgccgaa gcgggccgat
       61 tggccggcaa tcctcaggtc gcaggaacag gcgatttcca gtccggcacc tgcacaggcc
      121 ccctgaatcg ctgcaatggt gggctgggga agttgctgga tagcctgcaa cacctgccca
      181 accgactcgt gataagccag cgcgctatcc acggtggcac gtgcttcgcg caattcctct
      241 atatcgccgc cggcggcgaa tacctcgcct gcgccgcgca cgatcacgca acgaatatcg
      301 gcatcttccg caagccgcaa catggtatgc cgcagttcct gccacatggt ggcgttcatg
      361 gcgttgagct tgccaggatt ggagagtgtc actgtagcga tagggtctgt gcgatcacaa
      421 tgtacggtgg cgatagtcat cggtatgcgc tctttcatgg actggttgtg tggaagctac
      481 gttcgctcgc aaacgggacg cctaccgccg cttttgtggg ggccgatcaa cagctctgag
      541 ccgttccgac caatcgcaac ggcgtcgatc tttcatggcg aatcgcgtaa ttatgcgcct
      601 tctgacagct ccgcgggccg cttgtgcgct ttcacgtgat cttggcgaaa aggaaccgaa
      661 gttgatgccc attttctgtg cattattctc aaaaaatgtt gcagagcaac atcatcaggc
      721 gttagaatga aatcgcatat ttgggatcca ccagacttca gttgtagtga tgcagcctcc
      781 ctcttgcagc ggcttgtttc ctccggcgcg cgagggtttc ttggctggtt tgtgtcatta
      841 cgcaagcgcg cattcgtggc aacatgggcc attgtgcgtg ttgtacagca tcacgaaggg
      901 cgatcgaact tctgtccggt ccagtgaaat tcttgcaagc aacaatcaat atggtttttg
      961 gccacgatta gggagataac acatgaaact cacgctcacc catgatgaaa tgaatgccta
     1021 ttgcagcgag ctgcacgact ccaatgcggc attcaatatc cattatccgg cggattcttt
     1081 tcatcggcag ccggttcata cggtttacgg aggcgcgaac ctgttcaagg ccggattttc
     1141 cgccaagttg ggtgaggtcg cgctcaagac tctggaaacc catgcgccta cttacagtgt
     1201 ctttgcccgt gcgttgggta tgccgcgcgc ggatacgttg ccgacggatc ccaccgaact
     1261 ggcgaatctg acccgcgcgc tggagaccaa ccccgagctg gtacgcgaaa ccaaacaggc
     1321 cgcctggctg gcatatacgg tatataaccg cgtcgtgaag aagctgcaaa ccgagccgat
     1381 cgaggacaac cgcatcgatt ttgaggacgg ctatggtaat cgtcctgacg aagaggaaga
     1441 tgcgcatgcc atcgcggccg cagatgaagt cgccaaaggg atgagcgaga atattctgtc
     1501 gcccttcatc ggtatccgca tcaagacgtt ttcagatgag tgcaaggttc ggtcgatccg
     1561 cacgcttgac ctgttcctga cccgtctggc cgagaaaaca ggcagcaaac tgccaaacca
     1621 cttcatcgtt accctgccga aggtcaatac cccgactcag gtttccacat gcgtcaaggt
     1681 tctcgagaag ctcgaagcca agttgggctt tgccaaggat tcgctgaaga tggaaatcat
     1741 gatcgaaacg acccagtcga tcatcaatca caaaggagaa aacacggttc cgttgctggt
     1801 ggcggctgcc ggcgggcgtt gccgctcggc cattttcggg acgtacgatt acacagcctc
     1861 ctgcaatatt acggccgcgc accagaagca cacgcacccg gcctgcgact tcgcacgcca
     1921 tgtgatgcag gtcagcctgg cgggaaccgg catcaccatc agcgacggtg cgaccaccag
     1981 catgccgatc ggtccgcata aggccggtaa agacgacgtg ctgacttccc agcaaaggta
     2041 cgaaaaccgc atggtggttc atggcgcgtg gaagctgcac tacgacaata tcgtgcactc
     2101 gcttgcccat ggctactatc agggctggga tctgaatccg ggacagttgc cgattcgcta
     2161 tgcggccatg gatgcattct tcctcagtgg attgcaggat gcctcggctc gactgaacgc
     2221 attcctgaac aaggccgcgc aagccacgct ggtgggcaat accttcgacg atgcggcaac
     2281 cggccagggg ttgctgaact tcttcttgag aggaatggcg tgcggggcga tcaccgagca
     2341 ggaggttctg gcgacgggaa ttaccttgga cgaattgcgc tcccgttcgt ttgttcgaat
     2401 agtcaataat cggaccaaga aatagtggcc cattgaatgt aaatcaggga gatcagtcat
     2461 gaccgcggtc aagagtggaa tgcaaggtgc agtgcagcat gtcggatcag ccgacgcgct
     2521 gataaagaag tcgctgagct gggtgaagca gcaatgcctc ggcggcaagg gggtttcggg
     2581 cgaaaagctg gatgaacacc aaatggccag ctttgatctg gcctggtgtg ctgccgaggc
     2641 gactgcggcg cgttttgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     2761 tcagcagttt ctgtcagacg caactggacg caggcaatct ggatgcactg ggcaaactgg
     2821 tttctaccct ggatggcgcc agcggcgcct acctgctcgg cgacgatgca acgatgatgc
     2881 aggactcctt ccgcaagctg gcgacgaatg tggtgatgcc gctcgccgag gagattcatc
     2941 gggaagaccg gatcattccg ccggaaatct tggagccgct gaccgaaatg ggctgttttg
     3001 gcctgtcgat tcccgagcgg tacggtggca ttcagagcga cgatcatgaa gacaacatgg
     3061 ggatgatcgt ggtgactgag gagctttcgc gcggctcgct gggcggggcg ggcagtctga
     3121 tcactcgtcc ggagatcctc agccgatccc tgctcaaagg ggggaccgaa gctcagagag
     3181 aaaagtggtt gccacaagtt gcgatgggtc agcccttgtg tgccatcgcc gtgaccgaac
     3241 cggataccgg ttcggatgtc gctgcgatgc gcctcaaggc gacgaaatgc gacggcgggt
     3301 gggtgcttga cggcgccaag acctggtgca ccatggctgg aaaggcaggg ctgctcctgg
     3361 tcctggctcg ctccaacccc gacagttcgc tcggccacaa gggattgagc atgttcctgg
     3421 tcgagaagga ctcgtacgaa gggcacgact ttgaatatcg tcaaccgcag ggtggagtgc
     3481 tgacaggacg agcgattgcg acgattggtt atcgcgggat gcattcctac gaggtgtttt
     3541 tcgaccatgt gttcgttccg gacgaaaatc tggtcggcgg gccggaaggc gaagggaaag
     3601 ggttttactt cacgatggct ggatttgccg gcgggcgtat tcagaccgcc gcgcgtgcca
     3661 taggcgtgat gcaggccgct ttcgaaaagg ctgtttccta ttcgcaggag cggaaggctt
     3721 tcggcaagcc gatcggcgat tttcaactca cgcgcgtcaa gctcgcgcgg atggcgacgc
     3781 tgcttaccgt atcgcgccaa ttcacctacg ccgtgggccg attgatggac cagggtaagg
     3841 gtcagatgga agccagcatg gtgaaattgt tcacctgccg gacagccgag tggctcacgc
     3901 gtgaagcgat gcagattcat ggtggcatgg gttacgccga ggaaacgtcg gtcagccgct
     3961 atttcctgga tgcacgcgta ctgtcgattt tcgagggagc cgaggaaact ctggcgctga
     4021 aggttattgc gcgctcgttg attgacgctg tgtaagcagg tcttcaaacc gattcttgat
     4081 cagcaaaagg gaatagtcat ggcttctacc cagaaaaaaa cggatcgacc gtggttgatg
     4141 cggacctata gcgggcacag ctctgccaag gcgtcaaata ctctctatcg aaccaatctc
     4201 gccaagggcc agacgggact ctcggttgcc ttcgatctgc cgacgcagac gggctatgac
     4261 gctgacaatc cgttggccaa gggcgaggtc ggcaaggttg gcgtgcctat ctcgaacatc
     4321 gaggacatgg atcagttgtt cgatcagatt ccgctcgatc aaatgaacac ggccatgacc
     4381 atcaatgcca cggcgccatg gttattgtcg ctgtacatcg gattggcgca acggcggggc
     4441 atcgatccta aactactgag cggcacaacc cagaacgaca tcatcaagga gtatctctcg
     4501 cgcggtacct acatctatcc gccgggcccg tcgatgcgtc tgatcgccga tacgatcaac
     4561 tacaccgtaa agaatgttcc gaagtggaat ccgaccaata tctgcagtta ccatttgcag
     4621 gaagcgggtg cgactc
//
LOCUS       DAORPM010000193         4611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5539_length_4611_cov_7.290167, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000193 DAORPM010000000
VERSION     DAORPM010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5539_length_4611_cov_7.290167"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLD53_09255"
     CDS             <1..296
                     /locus_tag="PLD53_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61143.1"
                     /translation="LPAVIAQVHADAGTLLKNSAESSFDLVFLDSERSEYPAWWPDIK
                     RILRKGGLLVVDNATSHAAEMAPFAALVSADPDFTTCTVPVGNGEFLATRVSR"
     gene            492..1136
                     /locus_tag="PLD53_09260"
     CDS             492..1136
                     /locus_tag="PLD53_09260"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRC61144.1"
                     /translation="MGKLKAISISDVLFSGVQQKVLRLLFGQPDRSFYANELATLGMT
                     GRGALQRELQRMTSSGLISVTVIGRQKHYQANKNAPIYQELRGIVLKTFGLSDVLRHA
                     LSECAHNIAAAFVYGSVAKGTDTATSDIDVMVIADGLSYSHLFEDLTKAEEILGRKVN
                     PTLYSPEEFAKKFQNDNHFVVRVLDQPKIILIGDEDAISSGKLVELAEDREAQG"
     gene            1102..1509
                     /locus_tag="PLD53_09265"
     CDS             1102..1509
                     /locus_tag="PLD53_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61145.1"
                     /translation="MNLQKIGKLKAEPPDKGEFDGLVRSAKRKLPDAENTTLSPDSRF
                     VLAYDAAHSLALAALRWHGFRSENRYIVFQVLAHTLSLPTAKWRFLDNCHQKRNVALY
                     DGDYSEDEQLIEALIAAAKELQAAVEALGPIAP"
     gene            complement(1713..2852)
                     /locus_tag="PLD53_09270"
     CDS             complement(1713..2852)
                     /locus_tag="PLD53_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61146.1"
                     /translation="MSPPANAIALTSMLVRIWPYALPIVGLSFALAVHGFAAANPNAG
                     LREELAIIIGAVVLLVPSVLAALHHAESAARRIGEPYGTLLLTLSVTTIEVSIIVSMM
                     LHGTNNPTLAREAVFSAVMLVCSGGVGFCLMLGSLRHHEQGHNTQGTSAYLAVLLVLS
                     VLTLILPNFTLTTEAGTFSPGQLAFVSMLSVLVYIAFLYIQTVRHRGNFLDAGVQPPA
                     HHVAEQRHTSPLWANILFLLAGLAAVVLLAELVADGAERGLNAIGVLRPDAIVGAMIA
                     TLVLMPEFLTSLKAARLNHLQHSINAMLGSALASMALTIPAVVAVCLFTRRELILGLD
                     NRDATLLVLALTLCIVSFGTGRTNVLTGIVHLVIFFAYLMLLVIP"
     gene            2983..3417
                     /locus_tag="PLD53_09275"
     CDS             2983..3417
                     /locus_tag="PLD53_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRC61147.1"
                     /translation="MMSTLERAIVIAAEAHAGQMDKAGAPYVLHPLRMMLRLSTSDEM
                     IAAVLHDVVEDCGLSLEMLRADGFSEQVLEAIDSVTRRAEESYEEFVLRAASNSIGRR
                     VKLADLRDNSDLSRIKHPTARDYARVEKYRRAIETICALEGI"
     gene            complement(3469..4356)
                     /locus_tag="PLD53_09280"
     CDS             complement(3469..4356)
                     /locus_tag="PLD53_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRC61148.1"
                     /translation="MRKRLFAVALLAAAKLSLADVSVLDDTGATVRLAQPARRIVSLA
                     PHVTETLFAAGAGEHIAGTVEYSDYPEAAKKLPRVGGYSRFDIEAVAALKPDLIVAWQ
                     SGNSAAHVDKLRVLGFPLYISQPNRIDDVASEVERLGILAGTSTAGNAAARQFREHLA
                     GLQQRYAQRPRVRTFYQIWKQPLMTIGARQIISAVVSLCGGENVFAGLETFAPTVTVE
                     AVIAANPEAIVASGMGESRPEWLDDWKRWTSITAVARDNLFFVPPELIQRHTPRLLDG
                     AERLCQHLETARSRRQTAK"
BASE COUNT         1013 a         1271 c         1379 g          948 t
ORIGIN      
        1 gcctccccgc tgtcattgcg caggttcatg ctgacgctgg cacgttgctc aagaacagcg
       61 ccgaatcaag ctttgatctt gtttttctcg attccgagcg ctcggagtat ccggcgtggt
      121 ggcccgacat caagcgaatc cttcgcaagg gcggacttct cgtcgtcgac aacgccacct
      181 cccatgcagc cgagatggcg ccctttgcgg ctctggtttc cgcagacccc gacttcacca
      241 catgcacggt gcctgtcggg aacggtgagt ttctggcgac ccgggtttcc cggtaggcca
      301 tgccgcccgg cgcggttgtc atccgaactt gggcaataag cagcgcagat gggttctcat
      361 atgagaaccg caccagcaag gtttctcgac gtcgttcccc agaacaggga tgaacggccc
      421 tgttctgggg aatgcaaagt ctgttgtcgc caaagttgct attccccaaa tcaggaacga
      481 taatcctcat tatggggaaa ctgaaagcga tctcaatctc ggacgtcctg ttctcaggag
      541 tgcagcagaa ggtgctcaga ctgctatttg gccagcctga ccgctccttt tacgccaatg
      601 aactcgcaac gctcggcatg actggtcggg gggccttgca acgtgaattg cagcgaatga
      661 catcgtccgg cttgatcagt gtcacagtca taggccgcca gaagcattat caggcaaaca
      721 aaaacgcacc aatttatcaa gagctaagag gaatcgttct caaaacgttt ggcctatccg
      781 acgttctccg gcacgccttg tcggagtgcg cccacaatat tgctgccgca tttgtttacg
      841 gttctgtcgc caaaggcacg gatacagcaa ccagcgacat cgacgtgatg gtgattgccg
      901 atgggttgag ttatagccat cttttcgagg acctgacaaa ggccgaggaa atacttggcc
      961 gaaaagtaaa ccccacgctg tactcccctg aagagttcgc gaagaaattc caaaacgaca
     1021 accatttcgt agtccgcgtg cttgaccaac cgaaaataat cctaattggt gacgaagatg
     1081 caatctcctc gggaaagctt gttgaacttg cagaagatag ggaagctcaa ggctgagccg
     1141 ccagacaagg gagagtttga cgggctggtt cgatcagcca agaggaagct tccagacgct
     1201 gaaaacacca ccctgtcgcc cgacagccgg ttcgtcctag cctatgatgc agcgcactcg
     1261 ctcgcgttgg cagcacttcg ttggcatggt ttcagatcag aaaaccgtta catcgttttc
     1321 caagttttgg cccatacgct atcgttgcca acagccaagt ggcggtttct cgacaactgt
     1381 caccagaaga gaaacgttgc gctgtacgat ggtgattatt ccgaagacga gcagttgatt
     1441 gaggcactca tcgccgcggc caaggagctg caggcagcgg tcgaggcgtt ggggccgatt
     1501 gcgccctgac ccatttgctt cacccaaatg cctccgttcc gcattgactc ctcttcagcc
     1561 tcatactcat tctcgtcttc tcagagtcca aaatcggttt gcatttcagt gcgtacgcct
     1621 gactcctgcg accgcataga tcacatcgcc aatcaaagcg gcagtcgcca cggtgtgtgc
     1681 gaaagcagcc atacggacaa tagaatctgg cgttacggaa tgacaagcaa catcagataa
     1741 gcgaagaata tgaccagatg cacgatcccg gtcagcacgt tggtgcggcc ggtgccgaag
     1801 ctgacgatac acagggtcag tgccagcacc agcagtgttg catcgcggtt atccaatccg
     1861 aggatcagct cgcggcgcgt aaacaggcag acggctacga ctgccggtat ggtcagtgcc
     1921 atgctggcca gtgcagagcc aagcatcgca ttgatgctgt gttgcaaatg gttgaggcgt
     1981 gccgccttca gggacgtcag gaactccggc atcagcacca gcgtggcgat catcgcgccg
     2041 acgatcgcat ccggacgcag cacgccgatg gcattgagcc cgcgttcggc gccatcggca
     2101 accagctcgg caagcagcac taccgcggcg aggccggcga gcaggaacag aatatttgcc
     2161 cacagcggtg aggtatggcg ttgctcggca acgtggtgtg ccggtggctg aacacctgca
     2221 tcgagaaaat tgccgcgatg acgtaccgtt tgaatataga gaaaagcgat atataccaac
     2281 actgacagca tgctgacaaa ggcgagttga ccaggggaga aggtgccggc ttccgtggtc
     2341 agcgtgaaat tgggcagaat caaggtcagc accgataaca ccagcaatac ggcgaggtag
     2401 gcgctggtgc cttgcgtatt gtgcccctgc tcgtggtgac gcaacgagcc gagcatcagg
     2461 cagaagccga cgccgccgct gcaaaccagc atgactgccg aaaacaccgc ttcgcgtgcg
     2521 agtgtaggat tgttcgtgcc gtgcagcatc atcgagacga tgatcgacac ttcgatcgtg
     2581 gtgacggaga gcgtcagcag tagcgtgccg tacggttcgc cgatgcgtct tgcagcgctc
     2641 tcggcgtgat gaagtgcggc aagcacgctc ggcaccagca gtactacggc gccgatgata
     2701 attgccagct cttcgcgtag gccggcattc ggattcgcag cagcgaagcc gtgcacggca
     2761 agggcaaatg acaagccgac gatcggcagc gcgtatggcc agattcgcac cagcatgctg
     2821 gtaagcgcta tggcgttggc aggcggtgac atagaggctc cgggttcgct cgctggtagc
     2881 gatcagcgag tggagggctt gttggtcgcg ctattatcag caaccctgag ctgactgaca
     2941 atcgaccggc ttgagggcga gttgtgggct taaggaggat gaatgatgag tacgcttgaa
     3001 cgagcgattg tgattgcagc agaagcgcat gcgggccaaa tggacaaggc aggcgcaccc
     3061 tatgtgttgc atccgctgcg gatgatgctg cgtctgtcaa caagcgacga aatgatcgct
     3121 gccgtactgc atgatgtcgt tgaggattgt gggttgtcgc ttgagatgct gcgcgcggat
     3181 ggcttttccg agcaggtgct tgaagccatc gattcggtga cgagacgagc cgaagagtcc
     3241 tatgaggagt ttgtgctgcg tgcggcatca aactccatcg gccggcgggt gaaactcgct
     3301 gacctccggg acaacagtga tctgtcgcgc atcaagcacc cgacggcccg cgactacgca
     3361 cgcgtcgaga agtaccgtcg tgcgatcgag acgatttgtg ctcttgaagg aatctgattt
     3421 cctcgccgtc gtcattgttc gctcatcgca agatgcatta ctggggccct attttgccgt
     3481 ctgccggcgg ctgcgcgccg tttccaggtg ttggcagagc cgttcggcac cgtcaagcag
     3541 gcgtggtgta tggcgttgaa tcagttccgg cggcacaaag aagaggttgt cgcgtgcgac
     3601 agcggtgatt gatgtccatc gtttccagtc gtccagccat tccgggcgcg attcgcccat
     3661 gccgctggca acaatggctt cggggttggc tgcaatcacg gcttcgacag tgaccgtcgg
     3721 cgcaaaggtt tcgagcccgg cgaaaacgtt ctcgccaccg cacagactca cgacggccga
     3781 gatgatctgc ctggcgccga tggtcatcag cggttgtttc cagatctggt agaaggttcg
     3841 cacccgcggg cgctgtgcgt agcgctgctg caatcccgca aggtgttcgc ggaattgtct
     3901 ggcggcggcg ttgcctgccg tgctcgttcc ggcaaggatg ccgaggcgtt cgacttcgct
     3961 ggccacatcg tcaattcgat tcggttgcga gatgtagagc gggaaaccca gcacgcgcag
     4021 cttgtcgaca tgcgctgccg aatttccact ctgccacgcg acgatcaggt ccggtttgag
     4081 cgcagcgacg gcttcaatat cgaaacgcga gtagccgccg acgcgtggca gcttcttcgc
     4141 cgcttccggg tagtcgctgt attcgacagt gccggcgata tgttcaccag cacctgcggc
     4201 gaacagggtt tcggtaacat gtggagccag gctgacaata cgccttgccg gctgcgcaag
     4261 gcgtaccgtg gcgcccgtgt catcgagtac tgaaacgtcg gcaagggaga gtttggcagc
     4321 ggcgagcagg gcgacggcga acagtcgttt ccgcatgagt gaatccattg ggtgattgtt
     4381 tgtggaatga gtgaaagcgc gggcaagaaa tgaggtgggg caatacagtg cgggcatgag
     4441 aaaatgcggg caacaagact cggattctac gatgaattac gccctcgata cgcctgatag
     4501 cgcaccagaa agcgcacgca aaacccccac cctgatgatt cagggctgta catccgattc
     4561 cggcaagaca acgctggttg ctgcgctgtg ccgcattctg caccgccacg g
//
LOCUS       DAORPM010000194         4607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5548_length_4607_cov_8.247803, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000194 DAORPM010000000
VERSION     DAORPM010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5548_length_4607_cov_8.247803"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLD53_09285"
     CDS             <1..1074
                     /locus_tag="PLD53_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61149.1"
                     /translation="GIGVFLSKYLLTIGLPFETWLQQLMDCAERESDPDRFFYASCER
                     MLELPWVTGGAWAPASGAEAGSGSFGRVSPISHDFPDQPLVFTLYTRHKLSPALVWHF
                     HLLVKLSNEYYIAKQRARELQQMSYQRAVHETGARLTHDVKNLLQSLNNLCFMAQEPG
                     DMDGVRLNQLLQQQLPQITQRLQRALEKLQVPQALHGGAGGAPSGETSTGKVGLVIAE
                     VWWGALKQRYAHDNILFSPVVFDKTARLPSVLFDSVADNLLQNALFKQQHEDGLVEVR
                     VTITADAALLSVCDSGNVVREDVVGDLLRAPVSSENGLGIGLFHAARQAEGYGYELQL
                     ACNEAGKVCFELKRGSGAEAKAG"
     gene            complement(1095..2366)
                     /locus_tag="PLD53_09290"
     CDS             complement(1095..2366)
                     /locus_tag="PLD53_09290"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRC61150.1"
                     /translation="MSNQSGFLEGIRSAGILPTDSEDLRLQKSLLIFATGLISFASML
                     WLFIYWQLGPHFSSTIPFAFQLLLIGNLLVYMKTLNFDAFRLIQITLILFMPFVAQWS
                     MGNFISASGISLWALLAPIGAVLFIGPREAIAWFVAYLFLTALSGSFDYYLADALTAT
                     QIKVSTQTTVFFFALNFAGVSTIVFLLLRYSASEKQKIQAHLVEAHEQLQIEQDRSER
                     LLLNILPGSIAERLKSSNQTIADGFADVSVMFVDIVNFTKIAEGLSPQQVFSMLNRIF
                     SSFDELAEKYGLEKIKTIGDAYMVAGGLNDLRSDYSDALVDMALEMRDLLQNDFQVNQ
                     MHLEVRIGIGTGPVVAGVVGKKKFIYDLWGDTVNIASRITSEGVPGMVQVDEATHRRL
                     QQRFDFHEPQMIYLKGKGNMVVYRVIGRIAA"
     gene            complement(2659..3417)
                     /locus_tag="PLD53_09295"
     CDS             complement(2659..3417)
                     /locus_tag="PLD53_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRC61151.1"
                     /translation="MPKFAMPLIPALAKERPVASSSSGFTLVELAIVLIIVGLLLSSL
                     VSPLTAQIDQRNYNETEQQITEIREALIGFAVTNGRLPRPATSVSDGAENPAACADDA
                     ACTGFIPWTTLGIKRTDAWNKMVRYSVSPAYANATFSLTTQGSKKIQTRDNAGAISYL
                     IGSAAACSASSPCSPAVVYSAGKNNWGVTEDGTALADASTTNADEDANETATTLFFSR
                     NQSTVPTGGEFDDIVTWIPPYVLMNRMVSAGKLP"
     gene            complement(3393..4352)
                     /locus_tag="PLD53_09300"
     CDS             complement(3393..4352)
                     /locus_tag="PLD53_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61152.1"
                     /translation="MALLVLMLLVFLAGASWMLAQSGSMEGRNQVNKVTASAMAQAKE
                     ALIGRAATDVNRPGSLPCPDTNNDGVAELFAGVNCPAYIGRLPWKTLDLPELLDGNGN
                     RLWYALSSGLRDRDEAQPINPSTVLQITLDGSPPSIAAIIFSSGPPLPSQIGRPSNAI
                     ADYLDGSNNDGDNSYVSGPPSATFNDKTLVITREDIFRTVNQRVLAEIRGPDDNAPGA
                     PSYGLRRYHADNASFPWADSGSDGYGDVGVTVGNLPYYDLKLPVSLPLPPPPPSHPLP
                     YSWLNPNGWLPLLTFQRLSASSARIAIGTSTMDVIPCPSSPCP"
     gene            complement(4401..>4607)
                     /locus_tag="PLD53_09305"
     CDS             complement(4401..>4607)
                     /locus_tag="PLD53_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61153.1"
                     /translation="NGVTQNENDMAGSVSVTPSRRDPAKVTVHISEIPVANAKVGETV
                     NRDTGEATDLLDGGRIGTKSTAAK"
BASE COUNT          976 a         1175 c         1441 g         1015 t
ORIGIN      
        1 ggtatcggcg tatttctttc gaagtatttg ctgacaatcg gattgccctt tgaaacctgg
       61 ctgcaacaac tgatggactg tgcggagaga gagagcgatc cggacaggtt cttttacgct
      121 tcgtgcgagc gaatgcttga actgccctgg gtgacggggg gggcatgggc acctgccagc
      181 ggcgcggagg ccgggtcggg cagcttcggc cgggtatcgc ccattagcca cgactttccc
      241 gatcaacctc tggtattcac tctctatacg cgccacaaat taagcccagc tctggtgtgg
      301 cattttcact tgctggtaaa actgagcaac gagtactaca ttgccaagca gcgcgctcgc
      361 gagttgcagc agatgagcta tcagcgtgcg gtgcatgaaa ccggggcccg tttgacgcac
      421 gatgtaaaaa acctgcttca gtcattgaac aacctgtgtt tcatggcgca ggagcccggc
      481 gacatggatg gagtgcgctt gaatcagctg ttgcagcagc agttgccgca gataacgcaa
      541 cgtctgcaaa gagcgctgga gaagcttcag gtgccgcagg cgctgcatgg tggggctggc
      601 ggggcgccaa gcggtgaaac ttcgactgga aaagtcggac ttgttattgc agaagtctgg
      661 tggggcgcgc tgaagcaacg ttatgcgcat gacaacatac ttttcagtcc ggtcgtgttt
      721 gacaaaacgg ctcgcctgcc gtcggtgctg ttcgacagcg tggcggacaa tttgctgcaa
      781 aacgcgctgt tcaagcagca gcacgaggat ggtctggtgg aggtgcgtgt gacgatcacg
      841 gctgacgcag cattgcttag cgtgtgcgac agcggcaatg tcgtacgtga ggacgtggtc
      901 ggcgatctgc ttcgggcacc ggtatcgtcg gagaacggac tcgggattgg cctgttccat
      961 gcggcaagac aggccgaagg ctacgggtat gagctccagc tggcttgcaa tgaggccggt
     1021 aaggtctgtt tcgagttgaa gcgtgggagc ggtgcagaag cgaaagccgg ttgattgccc
     1081 ctgggggaaa tcgctcaggc tgcgatgcgg ccgataacgc ggtagacgac catattgccc
     1141 ttgcccttga ggtagatcat ctgtggttca tggaaatcaa agcgttgctg taagcgccga
     1201 tgggtcgctt catccacctg aaccatgccg ggaacccctt cgctggtgat gcggctggca
     1261 atattgacgg tgtctcccca caggtcataa atgaatttct ttttgccgac aacgcccgca
     1321 accaccgggc cggtgccgat tccgatacgt acttcgagat gcatctgatt gacctggaag
     1381 tcgttctgca agagatcccg catctccagc gccatgtcga ccagcgcatc ggagtaatcg
     1441 ctacgcagat cgttcaagcc tccggcgacc atataggcat cgccgatcgt cttgattttt
     1501 tccagtccgt acttttcggc gagttcgtca aaagacgaga atattctgtt gagcattgaa
     1561 aacacttgtt gcggactcaa gccctcggct atttttgtga agttgacgat gtcaacaaac
     1621 atcacggaga catcggcaaa tccgtctgca atggtttggt tgctgctctt caggcgttcg
     1681 gcgatagagc ccggcaggat gttcaggagg agacgttctg aacgatcctg ctcgatctgg
     1741 agctgctcgt gtgcttcgac gagatgagcc tggattttct gcttttcgct ggccgagtag
     1801 cgtaacagca gaaacacaat ggtcgaaacg cctgcgaagt tgagggcaaa gaagaacacc
     1861 gtggtctgcg ttgatacctt gatttgtgtc gctgtcagcg catcggcgag gtagtagtca
     1921 aaactgccgg acagcgcggt aagaaaaaga taggcaacaa accaggcaat agcctcgcgc
     1981 gggccgataa acaaaacagc gcctattggc gccaacagcg cccacaggct gatgccactg
     2041 gcagaaatga agttgcccat actccactgg gcaacaaagg gcatgaagag tatcagcgtg
     2101 atctggatca ggcgaaaggc gtcaaaattc agcgttttca tgtagaccag cagattgccg
     2161 atcagcagca actgaaacgc gaaggggatg gttgacgaaa aatgcggtcc gagttgccag
     2221 taaatgaaaa gccagagcat cgaggcaaaa ctgatgaggc cggtggcaaa aatcagcagt
     2281 gacttttgca gacgtaaatc ttcggaatcg gtcggcagaa ttcctgcgct gcgaatgcct
     2341 tcaaggaagc cgctttgatt gctcatggat gtctgacgct gtcattgagg aacagcgacc
     2401 gatttgactc caaacggtgg aggcggtcaa ctccgtcgtt tgcgcaagcc gtttgcattt
     2461 catgatgtct tgtgaggcgg gcaggatggg cgtctctcga tgctgcgctg cgagggtctg
     2521 cgttgtttat ggggtcgtca ggtcggggcg cgcttagaga cttggcgtcg tgtacagcga
     2581 cctcctggct catgcacggt gaaaaagcgc agcgggtccg cccgtaacaa agccgaggtt
     2641 tctttggaga cagtactctc agggaagctt gccagccgag accatgcggt tcatcagaac
     2701 gtaaggcgga atccaggtga caatatcgtc gaactcacca cccgttggaa cggtggattg
     2761 attgcgtgag aagaagagcg tcgtggcggt ttcattggcg tcttcatccg cgttggttgt
     2821 cgacgcgtct gcaagcgccg ttccgtcctc cgtgacaccc cagttgtttt ttccggcgga
     2881 atagacgacc gccggggagc agggtgaaga tgcgctgcag gcggcagcgc tgccgatgag
     2941 atagctgatt gccccggcgt tgtcgcgcgt ctggatcttc ttgctacctt gggtggtcaa
     3001 gctaaaggtg gcgttggcat aagctgggct gacgctgtag cggaccatct tgttccaggc
     3061 gtcggttctt tttatgccca gcgttgtcca ggggatgaaa ccggtacagg ccgcgtcgtc
     3121 tgcgcaagct gccgggttct ccgcgccgtc ggacactgag gtcgccggtc gtgggaggcg
     3181 gccgttggtg acagcgaaac cgatcagtgc ttcgcgtatc tcggtaatct gttgctcggt
     3241 ctcattgtag ttccgttgat caatctgcgc ggtcaaaggt gacaccaggc tgctcaaaag
     3301 cagtccgaca atgatcagca cgatggccag ttcgaccaga gtgaagccac tcgaactcga
     3361 tgcgacgggg cgttctttgg cgagtgcggg aatcaggggc atggcgaact tgggcatggt
     3421 atgacgtcca tcgtgctcgt gccaattgcg attctggctg aactcgcact gagtcgctga
     3481 aaggtgagca gtggtagcca accgttggga ttcaaccagg agtaagggag gggatgagag
     3541 ggaggaggtg gcggcagcgg cagtgagact ggcagtttca ggtcgtagta gggcaggttg
     3601 ccgacagtca cgccgacgtc accataaccg tcgctgccgc tgtcggccca ggggaagctt
     3661 gcgttgtcgg catggtaacg gcgcaaacca taggatggcg ctcccggagc gttgtcgtcg
     3721 ggaccacgaa tttccgccag aactcgctga ttgacggttc tgaagatgtc ctcgcgagtg
     3781 atgacaagag tcttgtcgtt gaaggtcgca gacggcggtc cggaaacata ggaattgtcg
     3841 ccgtcgttgt tgctgccgtc gaggtagtcc gcaatagcgt tgctcggcct gccaatttgg
     3901 ctcgggagtg gggggccgga ggagaagatg attgcggcaa tgcttggcgg cgaaccgtcg
     3961 agggtgatct gcaataccgt tgatgggttg attggctggg cctcatcgcg atctcgcaat
     4021 ccggaggaaa gcgcgtacca taacctgttc ccgttgccgt cgagcaactc aggcaagtcg
     4081 agcgttttcc atggcaagcg gccgatatag gcggggcagt tcacgccggc aaagagttcc
     4141 gccactccgt cattgtttgt gtcggggcag ggcagacttc cgggccggtt aacgtcggta
     4201 gcggcccgtc cgatcagggc ttccttggcc tgggccattg cagaggcagt caccttgttg
     4261 acctgattgc gtccctccat cgacccactc tgcgcaagca tccatgaggc accggcgaga
     4321 aagacaagga gcatcaggac aagtaaagcc accccacgtt ggcggcctga aaatgccaag
     4381 aaaagtcttg ggcgcatggt ctatttggcg gcagttgact tggtgccgat ccgaccgcca
     4441 tcgagcaggt cggttgcttc acccgtatct cggttaacgg tctcccccac cttcgcgttg
     4501 gccaccggaa tttcgctgat atgaacggta actttggccg gatcccttcg gcttggcgtg
     4561 acggatacac tgcccgccat gtcattttca ttctgtgtca cgccgtt
//
LOCUS       DAORPM010000195         4557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5659_length_4557_cov_11.805420, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000195 DAORPM010000000
VERSION     DAORPM010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5659_length_4557_cov_11.805420"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLD53_09310"
     CDS             complement(<1..738)
                     /locus_tag="PLD53_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61154.1"
                     /translation="MKLPASLSFSDVRPGVPGRVGRAVVLSLLALCLSLPAFSAHAQS
                     EAEDGGPASFDEVDASGIKLTAAEAARILALPVPEAADARYAALQRQYEAARLLEDQV
                     RLIATARQLIDAGRGHVGGEDWILAYLNAEFVWGSSGAALEACEPFIADTRLSLDTRA
                     AVALRQTYFAAQGHDRLLMNRFWSRADDLAKQAMKQATGMSPFLPVNRLQVRSEIERW
                     QGESAAAVATLREAVGVARLAVAATQAR"
     gene            1070..2086
                     /gene="fliD"
                     /locus_tag="PLD53_09315"
     CDS             1070..2086
                     /gene="fliD"
                     /locus_tag="PLD53_09315"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRC61155.1"
                     /translation="MSPLAIASTLFGLFQSSGSGSTAQSNQTGSGRHNAAESGDFSSS
                     LALRMAGAKSQSINILLGSALGGNKASSGTDFLTRLSGAQSGSQLGATDPLSLLGLST
                     TSQGLSPSGRNLSLFDPESAYRMMSVINTKDVTYKAQFSELSEMGTLVSGMQQAGQAL
                     GGVSALMDNEAIESQLQAFAAKYNDWVTRFDGTVKNNGLLAGTQAAEISLYELEQSVK
                     NIFNGAKDGFHGMRDLGLNIDPNTKLATLDTSQLFAALGTNKTGAINALHEFGANFAK
                     SAELLNSANNFIPNRLGNLDRVIDYITDHRSSLQAEFGLGDPAKPSAQVAKALAAYNQ
                     ILRT"
     gene            2260..3681
                     /locus_tag="PLD53_09320"
     CDS             2260..3681
                     /locus_tag="PLD53_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HRC61156.1"
                     /translation="MRIETQQNFLESRTFDEINVGDCSVLVRSLRAEDIKLFAIMSGD
                     MNPAIGDKEFSNSGMFREVIAHGMWSASLISTVLGTQFPGPGTILVDQALHFARPVSL
                     GDTITVTMTVKKMFTHNRHILFDCCCVNQDNLEVVRGTAEVLAPAEKVKTKRVDLPHI
                     TISDKYARLQHLVARANGLAPIDMAVVHPCDLESLKGALMAAEAELIDPILIGPEAKI
                     RGIASEHGLDLKQHRIVNVKHSHDAAAMAVSLIHNGDAEALMKGSLHTDELMAEVVSH
                     ASGLRTERRISHVFLMDVPTYPRPILITDAAINISPTLDDKVDIIQNAIDLAHIIGIP
                     EPKVAILSAVETVSAKIQSTLDAAALCKMADRGQIRGGLLDGPLAFDNAVSLEAAKTK
                     GIVSAVAGRAEILVVPDLESGNMVAKQLEYLANALTSGIVLGARVPIVLTSRADTAET
                     RIASCVIATLIAHANRGKQAKTE"
     gene            4046..>4557
                     /locus_tag="PLD53_09325"
     CDS             4046..>4557
                     /locus_tag="PLD53_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HRC61157.1"
                     /translation="MNQNAKQLSFTAIADSPGTAPLAEQEISGEVLIEKYAKGRETNV
                     HDVRRRVANALAATEVEKDRALWEERFLWAQENGFVPAGRINSAAGTPLAATLINCFV
                     QPVGDSIVEIVDGKPGIYSALAEAAETMRRGGGVGYDFSSIRPQGALVKGTQSRASGP
                     VSYMRVFDRS"
BASE COUNT          988 a         1451 c         1265 g          853 t
ORIGIN      
        1 gcgcgcctgt gtcgcggcca cagcgagcct ggcaacaccg accgcttcgc gcagcgtagc
       61 cacggcggcg gccgactcgc cctgccaacg ctcgatttcc gaacgcacct gcaaacggtt
      121 gacgggcaga aatggcgaca tgccggtggc ttgtttcatc gcctgttttg ccagatcgtc
      181 cgctctcgac cagaaccggt tcatcaatag ccgatcatgg ccctgcgcgg caaaataggt
      241 ctgccgcaaa gccaccgccg cacgcgtgtc gagcgacaac cgggtatcgg caatgaaggg
      301 ctcgcaagcc tcaagtgccg cgcccgaact gccccagaca aattcggcgt tcaggtaggc
      361 caggatccaa tcttcaccgc caacgtggcc acggccggcg tcaatcaact ggcgcgccgt
      421 tgcgatcaag cgcacctgat cctcaagcag gcgtgccgcc tcgtactgcc gctgcaatgc
      481 ggcatagcgg gcatccgcgg cttcaggtac aggcaaagcc aatatccgtg cggcctcagc
      541 ggcggtcagc ttgataccac tggcgtcgac ctcatcgaag ctcgccggac ccccatcctc
      601 ggcttcggac tgtgcgtgcg cagaaaacgc tggcaaggac agacacaagg ccagaagact
      661 cagcaccact gcgcgcccaa cgcgaccggg aacaccggga cgcacatcac tgaaagataa
      721 cgatgcgggc agcttcatgt ccgtctccaa tcaagggcaa tcggtcaaga ttcggcgtgc
      781 cgggattgag gactagcgac ggcacgctcg ggaggatctg cggaccggat gggctccgtc
      841 aattgaccat gctaattctt ttgggcggca ataattgccg gatagtcgtc ggtgattgag
      901 cttcccgaag ctgtccattt tcaggccgtg ttccgctacc ccagctccca gcccatcgtc
      961 gagttctcca gcctgggcat gcgagcgctg gcctgcatat tgcttctact tcagcaaacg
     1021 gcatccggat caccggtttg ccgatctgaa tcttgccgca ggagtcgcca tgtcaccctt
     1081 agccattgcc agcaccttgt tcgggctctt tcaatcgagc ggatccggca gcaccgccca
     1141 atcgaaccaa acgggctcgg gacgacataa cgctgcggag agcggcgatt tttcgtcgtc
     1201 gctggccttg cgcatggccg gggccaaatc gcaatccatt aatatcttgc tcggctcggc
     1261 actcggcgga aacaaggcgt cttcaggtac cgatttcctc acccgcttgt ctggcgctca
     1321 gtcgggatca caactcgggg cgaccgaccc gctttcactg cttgggctat cgacgacatc
     1381 acaggggctt tcaccttccg gccgcaatct atcgcttttc gacccggaat ccgcctatcg
     1441 gatgatgagc gtgatcaaca ccaaggatgt cacctacaag gcccagtttt cagaactgag
     1501 cgaaatggga accctggtat ccggcatgca acaggctggc caggcacttg gcggtgtcag
     1561 cgcattgatg gacaacgaag ccatcgagtc acagcttcag gcgttcgccg caaaatacaa
     1621 cgactgggtt acgcgttttg acggcacggt gaagaacaac ggccttctgg ccggaacgca
     1681 ggcggccgag atctcgctct acgaactcga acagagcgtc aagaacattt tcaatggtgc
     1741 gaaggacggc tttcatggca tgcgcgacct gggcctgaac atcgacccga acacgaaact
     1801 agccacactt gacacgagcc aactgttcgc ggcgctgggg acgaacaaga cgggagcgat
     1861 caacgccctt cacgagttcg gcgcaaactt cgccaaatcg gccgagttgc tcaactccgc
     1921 caataatttc attcccaacc gactgggcaa tctggaccgg gtcatcgact acattacaga
     1981 tcacagatcc tcgctccagg ccgagttcgg gctgggcgac ccggccaagc cttcagccca
     2041 ggttgccaag gcgcttgccg cctacaatca aatcctgaga acctgaaatc gcggcgtcag
     2101 ccggaggcct gccaataaag tggcagcacg tcccacggac tgacaggttt gaatcatatg
     2161 cgttatgatg ttggcttcag cggccgcatc atgggggagt ccagccacct gcacatcgcc
     2221 actccccgcc gcccactcat ttactgttcg ggctgaatca tgcgcataga aacccaacaa
     2281 aactttcttg aaagccggac cttcgacgaa ataaacgtcg gcgactgttc cgttctggtt
     2341 cgttcgctgc gcgccgaaga catcaagctg ttcgccatca tgtcgggcga catgaatccg
     2401 gcgatcggcg acaaggagtt ttccaacagc ggcatgttca gggaagtcat cgctcacggc
     2461 atgtggagcg catcgctgat ctccaccgtg ctgggaacgc aattccccgg accgggaacc
     2521 atcctggtcg atcaggcgct gcattttgcg cgaccggtca gcctgggcga cacaattacc
     2581 gtcaccatga cggtcaagaa gatgttcacc cacaaccggc atattctctt tgactgctgt
     2641 tgcgtcaatc aggacaacct ggaggtggtg cgcggtacgg ccgaggttct ggcgcccgcg
     2701 gaaaaggtga aaaccaaacg cgtcgacctg ccgcatatca caatctcgga caaatacgca
     2761 cggttgcagc acctggtcgc tcgggccaac ggactggcac ccatcgacat ggccgtggtt
     2821 cacccctgcg atctggaatc gctcaaaggt gcactgatgg cagcggaggc cgaactgatc
     2881 gatccgatcc tgatcggccc cgaagccaag attcgcggca tcgccagcga acacgggctg
     2941 gatctcaagc aacaccgcat tgtcaacgtc aagcatagcc acgatgccgc ggcaatggcc
     3001 gtttcgctga tccacaacgg tgatgccgag gcgctgatga agggcagcct gcacaccgat
     3061 gaattgatgg ccgaggtggt atcgcacgct tccggtctgc gtaccgaacg acgcatcagt
     3121 cacgtctttc tgatggatgt accgacctat ccgcgcccga tcctgatcac cgacgcggcg
     3181 atcaacatct caccgaccct cgacgacaag gtcgacatca tccagaacgc catcgacctc
     3241 gcgcacatca tcggcattcc cgaaccgaaa gtcgccattc tgtccgcagt cgaaaccgtc
     3301 agcgcgaaga ttcaatccac gctcgacgcc gccgcgctgt gcaagatggc cgaccgcggc
     3361 cagattcgcg gcggcctgct cgacggtccg ctggccttcg acaacgccgt gtcgcttgag
     3421 gccgcaaaga ccaagggcat tgtttccgcg gttgccggac gcgccgagat tcttgtcgtt
     3481 cccgaccttg agtccggcaa catggtggcc aagcaactgg aatatctggc caatgccctg
     3541 acttccggca tcgtcctcgg cgccagggtg cccatcgtgc tgaccagccg cgccgacacg
     3601 gccgaaacgc gaatcgcctc ctgcgtaatt gccacgctga tcgcccacgc caaccgcggc
     3661 aagcaggcca aaaccgaatg attccgagca tcctggtcgc cagcaatggc ggcgcttcgt
     3721 tcgagcaagc gcttcagtag aactctcctc gcgcgaccgg cgctcctgag cccggcttgt
     3781 gcagcgcagc aaaaatatat tttattcccc tcagcacagt gaattcacag tctttttccg
     3841 tctgctgcac tcaggccaaa atgactacat ttagttaatt tcttgcctat caccactgta
     3901 tgtagtagct tggtagctga ttcacaaaat aatcgccggc gcgatcagcc agctctctgc
     3961 agcacgaacg acagcagatt cacgttcatg gagtagctgt gcctttgacg ccgcaacgta
     4021 gtcacaaaaa cggaggagaa tggaaatgaa ccagaatgcg aagcaactga gctttaccgc
     4081 catcgctgac tcgccgggaa cggctccgct tgccgagcag gaaatttccg gcgaagtcct
     4141 gatcgaaaaa tatgccaaag gccgcgaaac caacgttcat gacgtacgtc gccgcgtcgc
     4201 caatgcgctg gccgccaccg aagtcgaaaa agaccgcgcc ctgtgggagg agcgcttcct
     4261 gtgggcgcag gagaacggtt ttgtccccgc cggacgcatc aactcggccg ccggcacgcc
     4321 gctggcagcc acgctgatca actgcttcgt acagccggtg ggcgactcga tcgtcgagat
     4381 tgtcgacggc aagccgggca tctacagcgc cctggccgaa gccgcagaaa ccatgcgccg
     4441 tggcggtggc gtcggctacg atttctcgtc gatccgccct cagggtgcgc tggtcaaggg
     4501 aacgcagtcg cgcgcttccg gcccggtctc gtacatgcgc gtgttcgacc gctcgtg
//
LOCUS       DAORPM010000196         4547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5683_length_4547_cov_11.114426, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000196 DAORPM010000000
VERSION     DAORPM010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5683_length_4547_cov_11.114426"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLD53_09330"
     CDS             <1..803
                     /locus_tag="PLD53_09330"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HRC61158.1"
                     /translation="RAISPASSRKYAEAPGNLDTPDNTEAGSDYVADMQLEEPSVADH
                     VEPPAESETEPVPGEEEIPLDETAAQELGKATGLILPRETTEIPGYSKWAEGAMFGPV
                     SVPGEKAPRWPFVLASLLLVLALVGQIVFRFRSEIAVTSPSLRPMLEAISGAFDSSLP
                     LPRHIELMSIETSDLQTDPAHGDLLILNATLRNRANYVQAYPSLELSLTDTQDAAIAR
                     RVFSPGEYLSPKIPADQPFAANADVAVRLWIEVKEISAAGYRLYVFYP"
     gene            901..>1447
                     /locus_tag="PLD53_09335"
     CDS             901..>1447
                     /locus_tag="PLD53_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRC61159.1"
                     /translation="MTTLICGSIAYDNIMVFHDRFKNHILPEQIHILNVAFLVPEMRR
                     EFGGCAGNIAYNLKLLGGEPLIMATVGDDADSYLLRLDDLGISRSHVRRIAGSFTAQA
                     FITTDLDDNQITAFHPGAMSFSHLNKVSDAKGATLGIVAPDGRDGMLKHANDLVAAGI
                     PFVFDPGQGLPMFSGEELLQFM"
     assembly_gap    1448..1547
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1548..1991
                     /locus_tag="PLD53_09340"
     CDS             <1548..1991
                     /locus_tag="PLD53_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRC61160.1"
                     /translation="DPGQGLPMFSGEELLQFMRLAEYACFNDYEAKLLCDRTGRSIEQ
                     LADEVCALIVTRGSEGSLIFAEGQRHEIPCVKADALIDPTGCGDAYRSGLLYGISEAW
                     DWVKTGRMAALMGAIKIGHRGGQNHRPSREEIAIRYEAAFGKAPW"
     gene            2003..2455
                     /locus_tag="PLD53_09345"
     CDS             2003..2455
                     /locus_tag="PLD53_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HRC61161.1"
                     /translation="MDKLKVLAVLLVSLVLAGCASSKSGDVYSRDQTRREMIVRTGVV
                     ESVREVTMEGTQSGAGTLAGAAVGGVAGSTVGGGKGQIVGAIIGAVLGGMAGTAIEEN
                     ATKKNALEITVSLDNGTLIAVVQEMGEIFHPGERVRVLSGSGSTRVTH"
     gene            2468..3265
                     /locus_tag="PLD53_09350"
     CDS             2468..3265
                     /locus_tag="PLD53_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRC61162.1"
                     /translation="MTKAGPTPYPIRCFRCLRLLLHLVWIGIGTFIFYPLIGEKRRAF
                     LKQRWSHQILKILSVHLETLPTDAPHGSLIVANHISWLDIFAVYALRPVAFISKAEIR
                     QWPFFGWLAKQNDTVFLRRGSRGHARIVNAEIDALLNTGMDVAIFPEGTTTDGTHMLG
                     FHAALLQPAIETGRPVLPLAISYHDADGRVSLAPSFAGDTTLAQCFSAILAQRSLTVR
                     LIPAPVIDSTKLHRRELAKVAHTAIAIKLGFLSSNNPPEKLPGPQSE"
     gene            complement(3201..4019)
                     /locus_tag="PLD53_09355"
     CDS             complement(3201..4019)
                     /locus_tag="PLD53_09355"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HRC61163.1"
                     /translation="MATYAIGDIQGCFDSFMRLLERCAFDPKHDRLWLVGDLVNRGPR
                     SLETLRFVRDLGPTAVAVLGNHDLSLLMAAEGFGKRGKGDTFDEILAAPDRDELLGWL
                     RHRPLCHVENQYCMVHAGLLPQWTASEARSLAAEVEAALTAADWREFMSQMWGSEPAS
                     WRNELEGWPRLRVIVNAMTRMRFCSPDGAMDFRFKGEVAKAPAGCMPWFQVPGRRSAD
                     SVLVTGHWSALGLKVTTNLMALDSGCLWGGSLSAIRFEDRAIFQVDCSKKEIRA"
     gene            complement(4107..>4547)
                     /locus_tag="PLD53_09360"
     CDS             complement(4107..>4547)
                     /locus_tag="PLD53_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRC61164.1"
                     /translation="KYEPTCDSADLAKCSQEAIVNTYDNALLYTDHMLAQTIAFLTKQ
                     DRKYDTAMIYLSDHGESLGENGLYLHGVPYSIAPKVQTEVPMVMWFSPGFSKSFSLDM
                     GCLRQRSDLPASHDNLFHSVLGLLDVRTSVYDSEMDLSATCRHQ"
BASE COUNT          968 a         1333 c         1234 g          912 t
ORIGIN      
        1 ttcgagcaat atcgccggcg agttccagga aatacgctga agctccagga aacctggaca
       61 cacctgacaa taccgaggct ggaagcgatt acgtcgccga catgcagctc gaagaaccct
      121 ccgtcgcaga tcacgtagaa ccgcctgccg agagcgagac ggaacctgta cccggcgaag
      181 aagagatccc actcgacgaa accgccgcgc aggagctggg caaagcaacc gggctcattc
      241 tgccacgcga gacgactgaa attcccggct acagcaaatg ggccgaaggc gcgatgtttg
      301 gcccggtctc ggtgccgggc gaaaaggcgc cccgctggcc tttcgttctc gcttcgctgc
      361 tgttggtgct ggcgcttgtc gggcagatcg tgttccgttt ccgcagcgag atcgctgtca
      421 cttcgcccag ccttcgcccg atgcttgagg ccatcagtgg cgcgttcgac tcaagccttc
      481 ctttgccgcg ccacattgaa ctgatgagta ttgaaacatc agatctccag actgacccgg
      541 cgcacggcga tctgctcatc ctgaacgcaa cgctgcgcaa ccgggccaac tacgtgcaag
      601 cgtatccttc acttgaactc tcgcttaccg atacgcaaga tgccgcaatc gcgcgccgcg
      661 ttttttcacc tggagagtac ctttctccca aaataccggc tgaccaaccc tttgcggcaa
      721 atgccgatgt cgccgtacgt ctgtggatcg aagtgaaaga gatcagcgcc gctggctatc
      781 gtctttacgt cttctacccc tgatcaggct tgcgattgtt gcctccgctg cagtccgcga
      841 gaccccgaag cccggtgcta taatttcgcg ctccaactcc tatagcattt caataaccct
      901 atgaccacgc tcatctgcgg ctccatcgcg tacgacaaca tcatggtttt tcatgatcgt
      961 ttcaagaacc atattttgcc ggagcaaatc cacattttga atgtcgcatt cttggtaccg
     1021 gaaatgcgcc gtgagttcgg agggtgtgcg ggaaacattg cctacaatct taagttgctg
     1081 ggtggtgaac cgctgatcat ggccaccgtt ggcgatgacg ctgattccta tctccttcga
     1141 ctggacgacc tggggatttc tcgctctcac gtgcgccgca tcgccggaag ctttaccgcg
     1201 caggcattca ttacaacgga tctggacgac aatcagatta ccgctttcca ccccggcgcc
     1261 atgagcttct cgcacctcaa caaagtttcc gatgccaaag gtgcgacgct gggcatcgtt
     1321 gctcctgacg gacgagacgg gatgcttaaa catgccaatg atctggttgc cgccggaatc
     1381 ccgttcgttt ttgacccggg acagggactg cccatgttct cgggcgagga actgctgcag
     1441 ttcatgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac ccgggacagg
     1561 gactgcccat gttctcgggc gaggaactgc tgcagttcat gcggctcgcg gaatacgcct
     1621 gtttcaacga ctacgaagcg aaactgctgt gcgatagaac cggccgttcg attgaacagc
     1681 ttgctgacga ggtctgtgcc ctgatcgtta cccgcggtag cgaaggctcg cttatttttg
     1741 ctgaagggca acgccacgag attccctgcg tcaaagccga tgcattgatc gaccccaccg
     1801 gttgtggcga cgcctatcgt tcaggcctgc tctatggcat ttccgaggcc tgggactggg
     1861 taaaaacggg gcggatggcg gcgctgatgg gggccatcaa aataggtcat cgcggagggc
     1921 agaaccatcg cccgagtcgt gaagaaatcg ccatacgcta cgaagcagcc ttcggcaagg
     1981 cgccttggta atgagggcga aaatggacaa gcttaaagta ctcgcagtac tactggtttc
     2041 gctggtgctg gcaggatgtg cgagcagcaa gtcgggcgat gtttactctc gcgatcagac
     2101 gcggcgcgag atgatcgtcc gcactggagt agtcgaaagc gtgcgtgaag tcaccatgga
     2161 aggcacacag tctggcgccg gaacattggc cggcgctgcc gtcggcggcg ttgccggcag
     2221 cacggtagga ggcggcaagg gacagatcgt cggcgccatc atcggcgcgg tgcttggcgg
     2281 catggcaggc accgccatcg aagagaacgc caccaagaag aacgcgctgg agattacggt
     2341 cagcctcgac aatggcacgt tgatcgcagt ggttcaggag atgggtgaaa tctttcatcc
     2401 gggcgaacgc gttcgcgtcc tgagtggcag cggttcaacg cgggttaccc attaacctgc
     2461 tgctttgatg accaaagccg gcccgacgcc ctatccgatc cgttgttttc gctgccttag
     2521 actgctgctc cacctggtgt ggattggtat cggcacgttt attttctacc cgctgattgg
     2581 cgaaaaacgg cgagcattcc tgaaacaacg ttggtcgcac cagatactga agattctttc
     2641 cgtccatctt gagacactgc cgaccgatgc acctcacggc agcctgatcg tcgccaatca
     2701 catttcctgg ctggacatct ttgctgttta tgccctgcgc ccggtcgcct tcatttccaa
     2761 ggcagagata cgccagtggc cattctttgg ctggctggcg aaacagaatg acacggtatt
     2821 cctgcgcagg gggagccgcg gtcatgcccg gatagtgaat gccgaaatag acgccttact
     2881 caataccggc atggatgtcg ccatatttcc ggagggaacg acaaccgacg gaactcacat
     2941 gcttggtttt cacgcggctc tgttgcagcc ggcgattgaa acagggcgac cggttttgcc
     3001 gctggcgatt tcctaccacg atgcagatgg gcgcgtcagt ctggcgcctt cgtttgccgg
     3061 agatacgacg ctggcacagt gtttttcagc catacttgcc cagcgctcac tgacggttcg
     3121 cctgattccg gcaccggtaa tcgactccac gaagcttcat cggcgcgaat tggccaaagt
     3181 ggcgcacact gccatcgcca tcaagctcgg atttctttct tcgaacaatc cacctgaaaa
     3241 attgcccggt cctcaaagcg aatagccgac agcgaaccac cccacaggca gccggaatcc
     3301 agcgccatca agttggtcgt gaccttcaag cccagcgccg accagtggcc ggtgaccaga
     3361 acggaatccg cgctgcgccg gcccggcacc tgaaaccacg gcatgcagcc ggctggcgcc
     3421 ttggcaacct cgcccttgaa ccggaaatcc atggcaccgt cgggcgagca gaagcgcatg
     3481 cgcgtcatgg cgttgacgat cacgcgcagg cgcggccagc cttcgagctc attccgccat
     3541 gaagcgggtt cactacccca catttgcgac atgaattcac gccaatcggc tgccgtcagt
     3601 gccgcctcga cttcggcagc caaagaccgc gcttcggagg ccgtccattg cggcagcaat
     3661 ccagcgtgca ccatgcagta ctggttttcg acatggcaca acggccggtg ccgaagccag
     3721 ccaagtagtt cgtcgcggtc cggagcggcc agaatctcat cgaaggtgtc acccttaccg
     3781 cgcttcccga acccctcggc agccatcaat agcgaaaggt cgtggttgcc gagaacggcg
     3841 accgccgttg gcccgagatc gcgcacgaaa cgcagtgtct ccaacgagcg cggaccccga
     3901 ttgacgagat cgccaaccag ccacagccga tcatgcttcg ggtcgaacgc gcaccgctca
     3961 agcaggcgca taaaagaatc gaaacaaccc tgaatgtcac cgattgcgta ggttgccaac
     4021 ccattctccc cgtgtagtcg tacagaatcc agcacctgtc gcacaaaaga tcaaccgcat
     4081 ctgtcgcaaa gcaaacctcc tgacaatcat tggtgtcgac aagtcgctga aaggtccatt
     4141 tcagaatcat agacgctggt acgtacatcc agcagcccga gtaccgagtg gaacagattg
     4201 tcgtgggatg caggcaaatc ggaacgctgg cgcaaacagc ccatatccag cgaaaaactt
     4261 ttagaaaatc cgggggagaa ccacatcacc atcggcacct cggtctgcac cttgggcgca
     4321 atcgagtacg gcactccgtg cagatacaac ccgttttctc cgagcgattc gccgtggtcg
     4381 gaaaggtaga tcatcgccgt gtcgtatttt cgatcttgct tcgtcaggaa ggcaatcgtc
     4441 tgcgccagca tatgatccgt atagagcaag gcattgtcgt aggtattgac gatcgcctcc
     4501 tgggaacact tggccaaatc tgccgaatcg cacgtcggct cgtactt
//
LOCUS       DAORPM010000197         4531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5713_length_4531_cov_9.702860, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000197 DAORPM010000000
VERSION     DAORPM010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5713_length_4531_cov_9.702860"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLD53_09365"
     CDS             complement(<1..236)
                     /locus_tag="PLD53_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61165.1"
                     /translation="MLEFVFSFATRFPFHMSSMTTLQPRRVMLRILATAAWAMSMFTS
                     TQAQEIEPRAYSNAPIGMNFFIAGYAYTDGDLPSG"
     gene            379..2496
                     /gene="ppk1"
                     /locus_tag="PLD53_09370"
     CDS             379..2496
                     /gene="ppk1"
                     /locus_tag="PLD53_09370"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRC61166.1"
                     /translation="MTYPPDIAHASGARFPSENFLNRELGILAFNRRVLSQARNPLVP
                     LLERLRFLCIVSSNLDEFFEIRVAGLKEQVKLNSVAVTTDGLTARAAYRLVSDETHSL
                     VEEKYALLNDEILPKLAEEGIVFLRRSAWTVEQREWIKDYFFREVMPVLTPIGLDPSH
                     PFPRVLNKSLNFAVELKGKDAFGRSSGAAIVQAPRVLPRVIRLPRELCDCAYGFVFFS
                     SVLHAFVGELFAGMNVLGCYQFRVTRNSNLFVDEEEVKNLRAKIQGELPQRHFGDGVR
                     LEVADICSDAMAEFLLSQFNLGATDLYRVPGPVNLVRLMQVPDWVERTDLKFTPFRPG
                     FPKSLHKGNSIFDDIRAGDTLLHHPYQSFSPVISLLELAASDPQVAAIKMTVYRTGTD
                     SVLMKSLILAAQNGKEVTVILELMARFDEEANIGWATKLEQVGAHVIYGVVGYKTHAK
                     MLLIVRREEGKTGSMLRRYVHLGTGNYHPKTARLYSDFGLLTCNEEIGDDVNEVFKQL
                     TGLGLAQSLKHLWQAPFSLQQNLLSAIQQEAEAARSGGRSRIIAKMNSLLEPGIISAL
                     YEASQAGVKIDLVVRGVCALRPGIKGLSENIRVRSIIGRLLEHHRVFYFYASGAEKVY
                     LSSADWMERNFFRRIELAFPILDKKLKRRVITEGLQIYFADNQQAWEMDANGSYRLRR
                     CARGKPRCAQSELIELLKPREPS"
     gene            2533..3447
                     /locus_tag="PLD53_09375"
     CDS             2533..3447
                     /locus_tag="PLD53_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HRC61167.1"
                     /translation="MSFHDDALDDSLTHALNAAIGDYLGAPCRIDQAQSVGGGSISRT
                     LIVRTGNTRWFVKLNRAKLHDMFAAEADGLSALGVCPALRVPRVVAHGVSGRQTYLVL
                     EHLSLRSLGDGPAGQNAGRALATLHRVKGEEFGWHRDNFIGSTPQLNASHHTWPLFFA
                     QQRLKPQLDLAKQNGHKGRIITDGERLLEKLPALFVNYQPSISLLHGDLWRGNAGLDE
                     SGKLALYDPAVYYGDRETDLAMAELFGGFPAGFYSAYRESWPLADGHEQRKTLYKLYH
                     VLNHLNLFGSGYLHQSKGMIDQLLAELG"
     gene            3611..4366
                     /locus_tag="PLD53_09380"
     CDS             3611..4366
                     /locus_tag="PLD53_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRC61168.1"
                     /translation="MHILGIDVGGTGIKAAVVDTTTGKLLTERTRTATPQPATPKAIG
                     KALKTILEQHQWSKEPIGMGFPAAIQKGIARTAANIDPSFIDYPVAEYFSKQTGCTVH
                     VANDADVAGLAEIRFGAGKGFTGVVLIVTIGTGLGTALFSNGHLLPNTELGHVMLDNG
                     FEAERYASESVRVLNNLKWGEWGPRFNHYLTTMESLLWPDLIVLGGGISRELHKFAPL
                     ITTKAPVIAADFLNQAGIVGAALFAEEQLRLTA"
BASE COUNT          997 a         1306 c         1253 g          975 t
ORIGIN      
        1 ccggagggca aatcgccatc cgtataggcg tatccggcaa taaagaaatt cattccaatc
       61 ggcgcgttgg aatacgcgcg tggctcgatt tcctgcgcct gcgtgctggt gaacatgctc
      121 atcgcccatg ctgcggtggc aagaattcgc agcatgacgc ggcgcggttg aagcgttgtc
      181 attgagctca tgtggaacgg gaaacgagtc gcaaaggaga aaacaaactc gagcatgagg
      241 atgtattttc gcagttattg cgatgcatgt ggttctgggt ttgcttgtgt atcacttaca
      301 gatatgtttc agtgatctac ttgaacgtaa cccaatcgtc gccttgcatc actcacccat
      361 tcctgattca atgaaagcat gacttatcct cctgatattg cgcacgccag cggggctcgc
      421 ttcccgtctg aaaacttcct gaaccgcgaa ctgggcatcc tggcattcaa tcggcgggtg
      481 ctttcacagg ccagaaatcc gctcgtccca ttgcttgaac gactgcgatt tctgtgcatc
      541 gtcagcagca atcttgacga attcttcgaa atccgtgtgg ctggcctcaa ggagcaggtc
      601 aagctgaatt ccgtagccgt taccacggac ggattgacgg cgcgtgcggc ctaccgtctg
      661 gtcagtgacg aaacacattc gctggttgag gaaaagtacg ccctgctcaa cgacgagatc
      721 cttcccaaac tggccgagga aggcattgtc ttcctgcggc gctcggcatg gaccgtcgaa
      781 caacgcgagt ggatcaagga ctattttttc cgcgaagtca tgccggtcct gaccccgatc
      841 ggtcttgatc catcgcatcc cttcccgcgc gtacttaaca agagtctcaa ttttgccgtc
      901 gaactcaagg gcaaggatgc cttcggccgc agctccggag cagcaatcgt ccaggccccc
      961 cgagtcctgc cgcgcgtcat ccggctacca cgtgaactgt gcgattgcgc gtacggcttt
     1021 gtgttcttct cgtcggtcct gcatgccttc gtcggtgagc tgtttgccgg catgaacgtc
     1081 ctgggctgtt accagtttcg tgtcacgcgc aacagcaatc tcttcgtcga tgaagaagaa
     1141 gtgaaaaacc tgcgcgcaaa gattcagggc gaactgccgc agcggcactt cggcgatggc
     1201 gtacggctgg aggttgccga catctgctcg gatgccatgg ccgagtttct cctctcccag
     1261 ttcaatctgg gagccaccga tctttaccgt gttccgggac cggtcaatct ggtgcgtctg
     1321 atgcaggtgc cggactgggt ggaacgaacc gacctcaagt tcacgccgtt caggccgggt
     1381 ttcccgaaat cgttgcacaa agggaattcc atctttgacg atatccgcgc cggcgatacc
     1441 ctgttgcacc atccctacca gagtttctca ccggtaattt cgctgctcga actggccgca
     1501 agcgacccgc aggttgcagc catcaagatg acggtgtacc gcaccgggac cgattccgta
     1561 ttgatgaagt cattgattct tgctgcgcaa aacggcaagg aagtcacggt gatcctcgaa
     1621 ctgatggcgc gttttgatga ggaagcgaat atcggttggg ccaccaagct agaacaggtc
     1681 ggcgcccatg tcatttatgg cgtcgtcggc tataaaaccc acgccaagat gctgttgatt
     1741 gttcgccggg aagaagggaa gaccggcagc atgttgcgtc gctacgtgca tctgggaacc
     1801 ggaaattacc acccgaagac cgcccgtctc tattcagact tcggactgct tacctgcaat
     1861 gaagaaatcg gtgacgatgt caatgaagta ttcaagcagc tcaccggact cgggctggcc
     1921 caatctctga agcatctctg gcaggctcct ttttccttgc agcagaacct gctgtccgca
     1981 attcagcaag aggccgaggc cgcgcgttcc ggagggcgtt cgcgcatcat cgccaagatg
     2041 aattcgctgc tcgaaccagg gatcatcagc gcgctttatg aggcatcgca ggccggagtg
     2101 aaaatcgacc tggtcgttcg tggcgtttgc gctttgcggc cgggaatcaa gggactgtcg
     2161 gagaatattc gggttcgttc aatcatcggc cggctgctcg aacaccatcg ggtattctat
     2221 ttctacgcca gcggcgcgga gaaggtttac ctgtcaagcg ccgactggat ggagcgaaac
     2281 ttctttcgcc gcatcgaact cgccttcccg atacttgaca agaagctcaa gcgtcgcgtg
     2341 attaccgaag gattgcagat ctatttcgcc gacaatcagc aggcctggga aatggacgcc
     2401 aacggcagct atcgactgcg ccgctgcgcg cgcggcaaac cgcgctgcgc gcaaagcgag
     2461 ttaatcgaac tgctcaaacc cagagaacct tcgtgatgca ctgactacgc agcagaccga
     2521 aacgggagtc cgatgagctt tcatgacgat gcgctcgatg atagtctgac ccatgccttg
     2581 aacgcggcaa tcggcgatta ccttggggca ccctgccgga ttgatcaggc acaatccgtt
     2641 ggcggcggtt cgatctcccg taccctcatt gtgcggaccg gcaatacgcg ctggtttgtc
     2701 aagctcaacc gtgcgaaact gcatgacatg tttgcggccg aagcggacgg cctgagcgcg
     2761 ctgggtgttt gcccggcctt gcgcgtgccg cgcgtcgttg cgcatggtgt cagcggccgg
     2821 caaacctacc tcgtcctcga acacctgagc ctgcgctccc tgggcgacgg cccggcaggc
     2881 cagaacgcag ggcgcgcgct ggccaccctg catcgcgtca agggcgaaga attcggctgg
     2941 catcgcgaca acttcatcgg cagcacgcca cagctcaacg ccagccacca cacctggcct
     3001 ctgttcttcg cacagcaacg gctcaagcca caactcgatc tggcgaaaca gaatggacac
     3061 aaaggaagaa tcatcaccga cggtgagcgt ctgcttgaga agcttcccgc actgttcgtg
     3121 aactatcagc cgagcatcag cctgctgcac ggcgatttgt ggcgcggaaa cgccgggctg
     3181 gatgaatcag gcaaactggc actgtacgat ccagcggtgt attacggcga tcgcgaaacc
     3241 gatctggcaa tggcagaact gtttggcgga ttcccggcag gtttctattc agcctatcgt
     3301 gaatcctggc cgctggccga cgggcatgaa caacgcaaga cgctgtacaa gctttatcac
     3361 gtcctgaacc acctcaacct tttcggtagc ggctacctgc accaatccaa aggcatgatc
     3421 gaccagcttc tggcagagct tggctgaaac caatcgatcg tcatcggcgt cccgtctttg
     3481 acgaagattc ggtccatcgg aaacttcgcc tgttttggga atcagtgccg cgtccgccca
     3541 cgccaaatgc actcaagaca cacaagatag gctagcattg gcccggaaca atttcgagtg
     3601 gagtcatagc gtgcatattc tgggcataga tgtaggtgga acgggaatca aggcagccgt
     3661 cgtagacaca acaactggca agctgctcac tgagagaacc cggacggcaa cgccccaacc
     3721 ggcaacgccc aaggccatcg gtaaagcgct gaaaaccatt ttggaacaac atcagtggtc
     3781 gaaggaacca atcggcatgg gctttcctgc cgccatccag aaaggaatag cccgcacagc
     3841 cgcgaatatc gacccttcct tcatcgacta tccggtagcg gagtattttt ccaagcagac
     3901 cggttgcacc gttcacgttg cgaatgacgc tgatgttgcc ggtctggccg aaatacgttt
     3961 cggtgcaggc aagggcttta ccggcgtcgt gctgatcgtc accatcggca ccggtctggg
     4021 cactgccctc ttctccaatg gtcacttgct gcccaacacc gaactcggtc acgtaatgct
     4081 tgacaatggt ttcgaggcag aacgatacgc ttccgaatcg gtgcgcgtct tgaacaatct
     4141 caaatgggga gaatggggac cgcgcttcaa tcattatctc accaccatgg agagccttct
     4201 ctggcccgac ctgattgtac tgggtggcgg catcagcagg gaactgcaca aattcgcacc
     4261 gctgatcacg accaaggcgc cggtcattgc cgccgatttt ctgaatcagg ccggtatcgt
     4321 cggtgccgcg ctgtttgccg aggaacagtt gcgcctcacc gcctgattgg ggttgaaaat
     4381 ggtatggcct gcttgagact tggcttcgaa atggcgcgac actgcgcggg ccgtttgaaa
     4441 agctttgcac cgcctggaga agaataagat gaagaccctg tttatccccg ctatctccct
     4501 gctcaaccgc ctgggctata cgaaaaaatt t
//
LOCUS       DAORPM010000198         4528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5722_length_4528_cov_8.375363, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000198 DAORPM010000000
VERSION     DAORPM010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5722_length_4528_cov_8.375363"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLD53_09385"
     CDS             <1..180
                     /locus_tag="PLD53_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61169.1"
                     /translation="TGSKGLSQQRNDGAAPLTNQAKLLELHALSNTGSRLMWPGRSLF
                     PTLRKIRLKYPPGFP"
     gene            221..1543
                     /locus_tag="PLD53_09390"
     CDS             221..1543
                     /locus_tag="PLD53_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HRC61170.1"
                     /translation="MRVKLLAALLPASAIFMAALPTVASAAWQLVTTEQGKRVEIDRA
                     SIVADPSGETMTRARIVLDKPIVDPKTSDSYRIIEVVNRYDCAQRTLATLRRSYFKEE
                     NDLLRSEEVKTPFAMPVRSGTADEKLLREVCRPKTAAGASGAVNSTLEKVNEAAGELR
                     KLNDELIEKSVQKELKELTSGAGSAGSAGSSVRAKRGAASGNPKKAAAPAATSMATHT
                     PWSYFGSGGPDNWSRLKSDYALCASGRRQSPIDIRDGIAVDLAPIQFLYRPAAFRVSD
                     SGRNMQVTVYGGGFNLLGKNYELIRVHFHRPSELTVNGKHFDMEAQLMHKSADGKLAI
                     VSVLLEKGIENPIVQMALNNLPLERNFEVSPPAERIDVSRLLPENRRYYTFMGSLTTP
                     PCTEDVRWLVLMHPQQISPDQLAIFERLYAPNARPVQPGFGRLIKESR"
     gene            1709..2605
                     /locus_tag="PLD53_09395"
     CDS             1709..2605
                     /locus_tag="PLD53_09395"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRC61171.1"
                     /translation="MPVNRLVILSLLAATVFSAPVLAQDAGTLKKIKDTGAISLGHRE
                     SSIPFSYYDDKQQVIGYSQEIMLKVVDAIKADLKLSKLEIKLLPVTSANRITLIQNGV
                     VDIECGSTTNNTERQKQVSFSNTIFIIGTRLLTKKDSGIKDFPDLAGKNVVTTAGTTS
                     ERLLRKMNEEKNMKMNVISAKDHGESFLTLETGRAVAFMMDDALLFGEIAKAKRPADW
                     AVVGTAQSEEAYGCMLRKDDPAFKKVVDTAIAKLMTSGEIVKIYDKWFLNPIPPKGLN
                     LNMPLSDDMKALYKSPNDKAFE"
     gene            2705..3442
                     /locus_tag="PLD53_09400"
     CDS             2705..3442
                     /locus_tag="PLD53_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRC61172.1"
                     /translation="MGYNWNWGVFLQPSATGDDTYLGWMLYGLKMTVALSLSAWIMAL
                     VLGAVIGVLRTVPNKALSGFAMSYVELFRNIPLLVQLFLWYFVLPELLPPSLGNAFKQ
                     SNPIVQQFLASMLCLGLFTSARVAEQVRSGINSLPPGQKNASLAIGFTLPQTYRYVML
                     PMAFRLVVPPLTSEFLNIFKNSAVCSTIGLLELAAQGRQLVDYTAQPYESFIAVTLAY
                     MLINVVVMFAMRWVEGRVRIPGYIGGK"
     gene            3444..4124
                     /gene="gltK"
                     /locus_tag="PLD53_09405"
     CDS             3444..4124
                     /gene="gltK"
                     /locus_tag="PLD53_09405"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HRC61173.1"
                     /translation="MYEFDWSSIPGALPYLAQGMRISLEITVTAILAGIVWGTLLAMM
                     RLSSIAPLSWFAAGYVNLFRAVPLVMVLLWFFLIIPHLLETLFGVPRGSDMRLGSAIA
                     GFSLFEAAYYSEIIRAGIQSVPKGQMAAANALGMTYGQTMRLVVLPQAFRNMVPLLLT
                     QGIILFQDTSLVYVSALADFFGAAYKVGDRDGRLVEMLLFAGAVYFVICFAFSQLVRH
                     YQKKLKTA"
     gene            4155..>4528
                     /locus_tag="PLD53_09410"
     CDS             4155..>4528
                     /locus_tag="PLD53_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC61174.1"
                     /translation="MISMNNVSKHYGAFQVLTDCTTHVNKGEVIVVCGPSGSGKSTLI
                     KCVNGLEPFQSGQITVNGVSVGDPKTNLSKLRAHVGMVFQNFELFPHMSIIENLTIAQ
                     IKVLGRSKPEAEVKGMQLLDRVG"
BASE COUNT          948 a         1295 c         1301 g          984 t
ORIGIN      
        1 acaggttcca agggactgtc tcagcagcgc aacgacggcg cggcacccct aacgaatcag
       61 gccaagttac tggaattgca cgctttaagc aataccgggt ctcgtttgat gtggccgggt
      121 cgaagtttgt tcccgacgct gcggaaaatt cgtctaaagt atccgccagg ttttccgtga
      181 gatggaagat gagcgtaaaa cgaatcactg gagtctgatc atgcgcgtca agcttctggc
      241 agcccttctg ccagcctcgg ccattttcat ggctgcgctt ccaacggtgg cctcggcagc
      301 ctggcaactg gtgaccaccg agcagggcaa acgcgtagag atcgaccggg cgagtatcgt
      361 cgcagaccca agcggggaga cgatgacgcg ggcgcgcatc gtactcgaca agccgattgt
      421 cgacccgaag acgtcggact cctaccgcat tatcgaagtg gtgaaccgtt acgattgcgc
      481 tcagcgcacc ctcgccacac tgagacgcag ctatttcaag gaagagaacg atttgttgcg
      541 ttcggaagaa gtgaaaacgc ccttcgcgat gccggtgcgc tcgggtacgg ccgacgagaa
      601 gctgttgcgc gaggtttgcc ggccaaaaac ggcagccggc gcctctggcg cggtgaattc
      661 gacgcttgag aaggttaacg aggccgcggg tgagttgcgc aagctgaacg atgagctcat
      721 cgaaaaatcg gtccagaagg agttgaaaga gctgacgtca ggcgctggct cggcagggtc
      781 ggcgggctca agcgtccgcg ccaaacgcgg cgcagcatcg ggcaatccca agaaggctgc
      841 cgcgcccgca gcaaccagta tggcgaccca cactccgtgg tcgtacttcg gcagtggcgg
      901 accggacaac tggagcaggc tgaagtccga ttacgcgctc tgtgcttccg gtcgccgcca
      961 gtcgcccatc gatattcgcg atggcattgc cgttgatctt gcacccattc agttcctgta
     1021 tcggcccgcg gcgttccgtg tttcagacag cggacgcaac atgcaggtga ccgtgtatgg
     1081 cggcggtttt aacctgcttg gcaagaacta cgagctgatt cgggtacatt ttcaccgccc
     1141 ctccgaactc acagtcaatg gcaagcactt cgacatggag gcgcaactga tgcacaagtc
     1201 tgcggacggc aaactcgcca tcgtctcggt gctgctcgag aaggggatcg agaatccgat
     1261 tgttcaaatg gccttgaaca acctgccgct cgaaagaaac ttcgaagttt cgccaccggc
     1321 ggaacgaatc gatgtatcgc gcctgctgcc ggagaaccgg cgttattaca ctttcatggg
     1381 gtcgctcacc acaccgccat gcaccgagga tgtgcgctgg ctggttctga tgcacccgca
     1441 acagatttcg ccggatcagc tggccatttt cgagcgcctg tacgcaccca atgcgcgacc
     1501 cgtgcagccg ggttttggcc gcctgatcaa ggaatcgcgc tagccggggc gaagatttgg
     1561 gcctgaacgc agcggcacca ggagctcggt caggttgacg tggtcatatt cagccgctat
     1621 cttgagcgtg gtgctttaaa gtggcacttt tccggcctgc gcctgtcaat acgtcgatca
     1681 atacaactac ctcaaggaga caatgatcat gcctgtcaat cgcttagtca tactatcgct
     1741 gctcgccgca accgtcttct ccgctccagt gttggcccag gatgcgggta cgctgaagaa
     1801 gataaaggac accggcgcca tcagcctggg gcaccgcgaa tcgtcgattc cgttctccta
     1861 ttacgatgac aagcagcagg tcatcggcta ctcgcaggaa atcatgctca aggttgtcga
     1921 tgccatcaag gccgacctca agctgtccaa actcgagatc aagttgctgc cggtgacttc
     1981 ggccaaccgc atcacgctga ttcaaaacgg tgtcgtcgat atcgagtgcg gatcgacgac
     2041 gaacaatacc gaacgccaga aacaggtttc cttctccaat accatcttca tcatcggcac
     2101 caggctgttg accaagaagg attcgggaat caaggatttt cctgatctgg cgggcaagaa
     2161 cgtggtgacc acggcaggga cgacgtccga gcgcttgctg cgcaagatga acgaagaaaa
     2221 gaacatgaag atgaacgtga tttcggccaa ggaccacggc gaatcgtttc tgaccctgga
     2281 aaccggccgc gccgtggcct tcatgatgga cgatgcactg ttgtttggcg aaattgccaa
     2341 ggccaagcgt ccagcagact gggcagtagt gggtaccgcg caatccgagg aagcctatgg
     2401 ttgcatgttg cgcaaggacg acccggcctt caagaaagtc gtcgatacgg caatcgccaa
     2461 actcatgacc tcgggagaga tcgtgaaaat ctacgacaaa tggttcctga accccattcc
     2521 gccgaagggc ctgaatctca acatgccact gtccgacgat atgaaggctt tgtacaagtc
     2581 gccgaacgac aaggcattcg agtaatctac gtcgaacagg ctgacagcgg tccgtcagcc
     2641 tgttcgactg cacaacgtcc cttaggggcc gtgttgcgtc aacgcgggat tcctggagtc
     2701 agccatgggg tataactgga actggggtgt cttcctccag ccgtcggcga cgggggacga
     2761 cacctatctt ggctggatgc tctacggcct gaagatgacc gtcgcgctgt cgctttcggc
     2821 atggatcatg gccttggttc tgggggcggt catcggggtc ctgcgtaccg ttccgaacaa
     2881 agcgctgtcc ggttttgcca tgtcctacgt cgaactgttt cgcaatatcc ccctgctggt
     2941 ccagctgttt ctctggtatt tcgtcttgcc ggaactcttg cctcccagcc tgggtaacgc
     3001 cttcaagcag agcaacccga ttgtccagca gttcctggcc tcgatgctct gcctcgggtt
     3061 atttaccagc gctcgcgtcg ccgagcaggt acgctccggg atcaattcgc tgccgcccgg
     3121 ccagaagaac gccagtctgg cgatcggctt cacgctgccc caaacgtatc gctacgtcat
     3181 gctgccgatg gcatttcgcc tggtagtgcc gccgctgact tcggaatttc tcaatatctt
     3241 caagaactcg gcggtgtgct cgaccatcgg tctgctcgag cttgccgcac aggggcggca
     3301 actggtcgat tacacggcgc agccctacga gtcgttcatc gcggtgacgc tggcctacat
     3361 gctgatcaac gtcgtcgtga tgtttgccat gcgctgggtt gaaggccgtg tccgaattcc
     3421 cggctatatc ggaggcaaat gacatgtacg aattcgactg gtcttcgatt cccggtgcct
     3481 tgccctatct tgcgcagggc atgcggattt ccctggaaat caccgtaact gcgattcttg
     3541 ccggcatcgt ctggggaacg ctgctggcca tgatgcggct gtcgtcgatc gcacctctgt
     3601 catggtttgc cgcaggctat gtcaatcttt tccgcgccgt gccgctggtc atggtgctgt
     3661 tatggttttt cctgatcatt ccgcatctgc ttgaaacgct gttcggcgtc ccgcgcggca
     3721 gcgacatgcg ccttggatcg gcgatcgccg gattttcgct gttcgaagcc gcctattatt
     3781 ccgaaatcat ccgtgccggg attcagtccg ttccgaaagg tcagatggcg gcagccaacg
     3841 cgctgggaat gacttacgga cagacgatgc ggctcgtcgt tctcccccag gccttccgca
     3901 acatggtccc cttgctgctg actcaaggca tcatcctgtt tcaggacacg tcgctggttt
     3961 acgtatctgc gcttgccgat ttctttggcg cagcctacaa ggtcggtgat cgtgacgggc
     4021 gactggtcga aatgctgctt ttcgccggcg ccgtttattt cgtgatctgt tttgcctttt
     4081 cgcaactcgt gcgccactac cagaaaaaac tcaagacggc ttgatgttcc gacaggcgcc
     4141 ccaggagaac accgatgatt tcaatgaaca atgtcagcaa gcattacggt gcttttcagg
     4201 tgctgaccga ctgtacgacg cacgtgaata agggcgaagt gatcgtggtc tgcggtcctt
     4261 cggggtcggg gaaatcgacg ctaataaaat gcgtgaacgg tctggagccg ttccaaagcg
     4321 gccagatcac ggtgaacggc gtttcggtcg gcgatccgaa aaccaacctg tcgaaactgc
     4381 gtgcgcatgt ggggatggtt ttccagaact tcgaactgtt cccgcacatg tccatcatcg
     4441 agaatctgac catcgcccag atcaaggtgc ttggccgtag caagccagag gcagaagtaa
     4501 agggcatgca gcttcttgac cgcgtcgg
//
LOCUS       DAORPM010000199         4505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5785_length_4505_cov_9.039101, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000199 DAORPM010000000
VERSION     DAORPM010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_4505_cov_9.039101"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLD53_09415"
     CDS             complement(<1..524)
                     /locus_tag="PLD53_09415"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HRC61175.1"
                     /translation="MRDCRELFQRRLAEIARQSGISSPSVIDAFSREIGDAHDELASS
                     DQQDGFEQTNGLTASRITLVGNDDLELDIRIGEIANRLKGNERIGHWRVQLRYMTLLQ
                     RPKMTAENNPVGLEPISRGLWAICRESGNKLDQNLDQLDRLEAKLLALLPDVYVELNG
                     VLERHRVEPAQVKIV"
     gene            complement(580..1512)
                     /locus_tag="PLD53_09420"
     CDS             complement(580..1512)
                     /locus_tag="PLD53_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRC61176.1"
                     /translation="MSQKKYRLVTRSDFDGLVCAVLLNELELIDDIKFVHPKDMQDGK
                     IDISDRDITTNLPYVATAHLAFDHHLSETIRVAGDRDNRVIDPDAPSAARVVYDYYGG
                     KKGFPRISDDMMDAVDKADSAQFTRDEILDPKGWVLLNYLMDSRTGLGRFRDFRVSNY
                     TLMMDLIKYCRSNGIDDILKLPDVKERVELYCEHAVQAKAQIQRCATVHNKLVVLDLR
                     NEDTIYATNRFMIYALFPQTNISIHVLWGVQKQNTVFATGKSILDRSSGINVGELMLK
                     YGGGGHHAAGTCQVENDQADAKLKEIIAQINANA"
     gene            complement(1607..1951)
                     /locus_tag="PLD53_09425"
     CDS             complement(1607..1951)
                     /locus_tag="PLD53_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61177.1"
                     /translation="MSAAALLLLLSGCDMIQQQLGLEDPNEKAARTDAEGRAVGGGCR
                     QSGRAIEDCYTIYSWLPKSPIYEGWRDMDAYMRENKIETIEPQLPPAPAPGTRRKIPP
                     PKSSAANATSGK"
     gene            complement(2065..2808)
                     /locus_tag="PLD53_09430"
     CDS             complement(2065..2808)
                     /locus_tag="PLD53_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61178.1"
                     /translation="MNKYQRLVVVAATMNVLVMLLFPPFTSQPLAKGVLPSFDGFYPI
                     VSQLGHKPLFTELLTIQLMFVGINTLVAWLALQTKTRHGKHPDFRFAQAIGWFVAVNL
                     IVIFTFPPFEPYQLLLRADAGGFDSFYFVLGSRSQRTIYWPLLYLECMLVAINALGIF
                     LMLSSVQRGADDIRRKLAQLADDLPEEAIERIAADIRQTINAQPPKPSASLGRGADRR
                     RTKRKAIAKERRSGKDRRHHSSSYTGDRP"
     gene            2956..4239
                     /locus_tag="PLD53_09435"
     CDS             2956..4239
                     /locus_tag="PLD53_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRC61179.1"
                     /translation="MNLSFLFMAMGGLILTAPAISAPARASAPAADPVQIELSQQLDE
                     ERAERLESLVERFNSQQKNVHLKTVRRIDGDAPKQINLVSFVDHARFITDKVKFKPLH
                     DVMREAKQPLDTSKLSPDLSHGLTDAKGRLYALPVAYSTPVLYYNKAAFRKAGLSPEK
                     PPKSWVEAQEVAGKLADSGSRCPFTTSWPAWVLIDNMSAWNGAPVNDAKGNLAFNGLV
                     QIKHVAMMATWHKSKYFSYFGRRDEADRRFANGECAMLTSTSSLNSALAENKSLEYGV
                     SALPYHDDVQGSPRNTLADGASLWFANGMSPAETKAVAEFIHYIMGPEMQIDLTLSGS
                     FLPITSVARAAASSKLLNENLAGLKVAYVELQGTVSSPTVRVAQDEKLRTIVEEELET
                     VWANKKPAKEALDDAVSRGNAIMEPVQVAKVKRKK"
     gene            4236..>4505
                     /locus_tag="PLD53_09440"
     CDS             4236..>4505
                     /locus_tag="PLD53_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRC61180.1"
                     /translation="MNRPAPGWTLPRVFAHRCGGALAPENTLAGLRMAAAMGCRAVEF
                     DVMLSADSSPWLIHDETLERTTNGAGRVCDTSDDALRKLDAGSYQH"
BASE COUNT         1004 a         1245 c         1253 g         1003 t
ORIGIN      
        1 acgatcttga cttgcgccgg ctcaacgcgg tggcgctcaa gcacgccgtt cagttccaca
       61 tagacgtcgg gaagcaacgc cagaagcttt gcctcaagcc ggtcaagctg atcaaggttc
      121 tgatcaagct tgttgccgct ttccctgcag atcgcccaca agccgcgact gatcggttcg
      181 aggccgaccg ggttgttttc cgcagtcatt ttcggtcgct gcaacaaggt catgtagcgt
      241 agctggactc gccaatgacc aatgcgctca ttgcctttca gacgattggc gatttccccg
      301 atacgaatat ccagctcaag gtcgtcattg ccgacgagcg tgattcgcga agcagtcagc
      361 ccgttggtct gttcgaatcc gtcttgctgg tcggacgagg ccagttcgtc gtgagcatcg
      421 ccgatttcgc ggctaaaagc atcgatgacc gatggggatg aaatgcccga ctgccgggca
      481 atctccgcga gtcggcgctg gaagagctcg cgacagtcgc gcaagatgaa aaacgaatcc
      541 ccgggtgctt gctgagctga acttgaggaa tcggtctgat caggcattgg cgttgatctg
      601 cgcaatgatt tccttgagct tggcgtctgc ctgatcgttt tccacctgac aggtgccggc
      661 cgcgtgatga cctccaccgc cgtacttgag catcagctcg ccaacattga tcccgctgct
      721 gcgatcaaga atcgatttgc cggtagcgaa gacggtgttt tgcttctgta cgccccacag
      781 aacatggatc gaaatatttg tttgcgggaa gagcgcgtag atcatgaagc gattggtcgc
      841 gtagatcgta tcctcgttac gcaaatcgag tactaccagc ttgttgtgaa ccgtggcaca
      901 acgttgaatc tgcgctttgg cctggacggc atgctcgcaa taaagttcta cgcgttcctt
      961 gacgtcaggt aatttcagaa tatcgtcaat accgttgctg cggcagtatt tgatcagatc
     1021 catcatcagc gtgtagttgc tgacacggaa atcacggaaa cgtccaagac cggtgcgcga
     1081 atccatcagg tagttgagca atacccagcc cttcgggtcg agaatctcgt cacgggtgaa
     1141 ttgcgccgag tcggccttgt cgacagcatc catcatgtcg tcggaaatcc gcgggaatcc
     1201 cttttttccg ccgtagtaat cataaacaac gcgcgctgcc gagggtgcgt ccgggtcgat
     1261 gacgcggttg tcgcgatcac cggcgactcg gattgtttct gaaagatgat ggtcaaaggc
     1321 gaggtgggcg gtggcaacat aaggcaggtt ggtcgtaatg tcccgatcgg aaatatcgat
     1381 cttgccgtcc tgcatgtcct tgggatgaac gaattttata tcgtcgatga gttcgagttc
     1441 gttgagcagc acggcgcaca ccaggccgtc aaaatcgctg cgcgtaacca atcgatactt
     1501 tttctgactc attacagact cctgttcttc aatctttcca ggtcgataac actatggagt
     1561 gaatacgcgt caatgtcaga acaatgcttc agcgtttgtc ctcagatcac ttgccagatg
     1621 tcgcgttagc tgcagagctt ttgggaggtg gaatttttct tcgggtgccg ggcgccggtg
     1681 cgggaggaag ttgaggttca atggtctcaa tcttgttttc gcgcatgtag gcatccatgt
     1741 cgcgccagcc ttcatagatg ggtgatttgg gaagccagga ataaatggtg taacagtctt
     1801 cgatggcacg ccccgactga cggcagccgc cgccgacggc tctgccttca gcatcggtac
     1861 gggctgcttt ttcgttcggg tcttcaagcc cgagttgttg ctggatcatg tcgcaaccag
     1921 acaggagtaa taacagagcg gcggcgctca cgaacagtcg cagtgcgctc cctagcttgg
     1981 ctgccacgag tatccctgac tattggtttg cataatcgcc attttaacca taaaaccctg
     2041 cgccccggtc aggagacatc gtcatcatgg cctgtcgcca gtgtagctgg agctatggtg
     2101 acgacggtct tttccgctcc gccgttcctt ggcaatggcc ttgcgctttg tgcggcgacg
     2161 atcggcacct cgtcccagtg aggccgacgg tttcggcggc tgcgcattga tcgtttgccg
     2221 gatatctgca gcgattcttt caatcgcttc ttccggaagg tcatcggcga gctgagccag
     2281 cttgcgccgg atatcatcgg cgccgcgctg cacggagctg agcatcagaa aaataccaag
     2341 cgcatttata gcgaccagca tacattcaag atagagcagc ggccaataga tcgtgcgctg
     2401 ggagcgactg ccgagaacga aataaaagct atcgaaacca cccgcatcgg cacgcagcag
     2461 tagctgatag ggttcgaatg gtggaaaggt gaaaatgact atcaggttta ccgcaacaaa
     2521 ccagccaata gcctgggcga aacggaaatc cgggtgcttg ccatgacgcg tcttggtctg
     2581 caaagccagc caggccacca acgtattgat gccgacgaac atcaattgta tggtcaacaa
     2641 ttccgtgaac aggggtttgt gccccagctg gctgacaatc ggataaaagc cgtcgaagct
     2701 tggcaacaca cccttggcca gtggctggct tgtgaacgga ggaaacaaca gcatgaccag
     2761 tacattcatc gtggcggcaa caacaaccag gcgctgatac ttgttcatga gggattctcc
     2821 gggatcgctc agatacggca gatgcgaact atagcgtgct ttgaaatcaa agtcctgcgc
     2881 gtatacattg catgatgccc acaaacgctt cagccactat aattcaccct ttgtcgccga
     2941 caccaaggag atatcatgaa tttatccttt ttattcatgg ctatgggcgg gctgattctc
     3001 accgctccag ccatttccgc tcccgcccgt gcttctgccc cggctgccga cccggtccag
     3061 atcgaactct cgcaacagct tgacgaagaa cgtgcagaac gtctcgaatc tctggtcgag
     3121 cgtttcaaca gccagcagaa aaatgttcac ctgaagacgg tgcgtcgtat tgatggcgat
     3181 gcgccaaagc agatcaatct cgtttcattc gtggatcacg cccgtttcat aaccgacaag
     3241 gtgaagttca agccactcca tgatgtcatg cgtgaggcca aacagccgct cgacaccagc
     3301 aagttatccc ccgatttgag ccatggcctg accgatgcca aaggccgcct gtacgccctg
     3361 ccggtggctt attcaacgcc ggtgctttat tacaacaagg ccgccttccg caaggccgga
     3421 ctgagtcccg aaaaaccgcc gaagagctgg gtcgaggcgc aagaggtcgc aggcaaactg
     3481 gctgattccg gaagtcgctg cccattcacc acatcctggc cagcctgggt gctgatcgac
     3541 aacatgagcg cctggaacgg tgcaccggtg aacgatgcca agggcaatct cgccttcaat
     3601 ggcctggttc agatcaagca cgtcgccatg atggcgactt ggcacaagag taagtacttt
     3661 tcctatttcg gccgccgcga cgaagcggat cgccgttttg ccaacggcga atgcgccatg
     3721 ttgaccagca cctcatcgct caatagcgcc ctggccgaaa acaaatcgct ggagtatggc
     3781 gtttccgcgc tgccctatca tgacgatgtg caaggctcgc cgcggaatac gctggccgac
     3841 ggcgcttcgc tgtggtttgc caatggcatg agcccggcgg aaaccaaggc cgtcgcagaa
     3901 ttcattcatt acatcatggg tccggaaatg cagattgatc tgacgctttc cggcagcttt
     3961 ctgccgataa cttcggtagc tcgcgcggct gcaagctcca aattgctgaa tgagaacctt
     4021 gccggactga aagtcgccta tgttgagctg caaggcacgg tttcatcgcc cactgttcgc
     4081 gtagctcagg acgagaaact gcggacgata gtggaagaag aacttgaaac agtctgggcg
     4141 aacaagaagc ccgccaagga agctctggat gacgctgttt cgcgcggcaa tgccatcatg
     4201 gagcccgttc aggtggccaa agtcaagcgc aagaagtgaa tcgccccgca cccggctgga
     4261 cactgccccg cgtctttgca catcgctgcg gtggtgcgtt ggcgccggag aacaccctgg
     4321 ccgggctgcg catggccgcc gcaatgggat gtcgtgctgt cgagtttgat gtcatgctgt
     4381 cggcggacag ctcgccttgg ctgattcacg acgaaacgct ggaacgcacc acgaacgggg
     4441 ccggtcgcgt gtgcgacacc tcagatgacg ctctaaggaa acttgacgcc ggcagctacc
     4501 agcat
//
LOCUS       DAORPM010000200         4497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5803_length_4497_cov_7.139577, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000200 DAORPM010000000
VERSION     DAORPM010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5803_length_4497_cov_7.139577"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1164)
                     /locus_tag="PLD53_09445"
     CDS             complement(34..1164)
                     /locus_tag="PLD53_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRC61181.1"
                     /translation="MKYITNIEQIEQIPKAEREKLKAVADTHSFRVSEYYLGLIDWND
                     PADPIRRLVIPNIAELTNWGHLDASNEAANTVVQGVQHKYPDTALLLCNEACGSYCRY
                     CFRKRLFMNDNEEAVKDIGPGLDYVRQHPEISNVLLTGGDPLLLSTGRLTDMIESIRA
                     IDHVQIIRLGTKMTAFNPWRILDDAPLLELLSTHSTPYKRIYFMNHFDHPRELTDPVI
                     ECLDKLIRAGVILTNQCPLVAGINDDPEVLSELFRKLSWIGCPPYYLFQGRPIAGNEP
                     FKVPIVRGWEIFREALRHGAGLARRARFAMSHETGKIEILAVDDKHIYLRYHRAKDAN
                     LRGRFLVYERDDNACWLDDLVPVGDAPKFARESLTDVLSGPE"
     gene            complement(1361..3532)
                     /locus_tag="PLD53_09450"
     CDS             complement(1361..3532)
                     /locus_tag="PLD53_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRC61182.1"
                     /translation="MSSTETASPQRSPLHDFLAPDSIAIVGASADPTKRGYKAMVGLV
                     KDGYQGTIYPVNPKAKMILGVKAYPSLADLPASPDLALICTPAAMAPALLVECGKKGV
                     KGAVILASGFRETGPEGAKLEQQLLDAARQGGVRVIGPNTSGMFNLHKKLNLLDLANV
                     KAGDIGFISQSGNMLLSLVLEAEHNGHVGFSTYVGPGNQTDIGFNDYLRYLSEDPNTR
                     VATFYVEGFRDGRRFLNVAREVTATKPVVVYKSGSTEQGRKAASSHTGALAGSYAMTV
                     DVLRQVGVSVVQHSDEILPVAEGLGLLQQARGKRVAVISDGGGQATIASDRLTEAGLE
                     LSVLSDATRHRLGEILLPQASLVNPVDVAGSTDVRPSLLAECMEILNEDDNVDSIFLV
                     GMFGGYHIRFAEHLLGDEMRGAESMIELAHKTDKPLVIYSLYAPISPPALRRLHEAGL
                     PVYNSIEHAVRVLAALGERGIYLAASAEHTHLAAVTPDVEVQATFAKALKEGRDLFEY
                     EAKSLLRTHGVAMPAEMVVREADKLTEVAAHFGSQPLVMKVVSKDILHKSDAGGVKLN
                     LRGETALREAYGSIMESCRDYSPDAVIEGMLVTPMARKGTEVIIGVMRDPIFGPVLMF
                     GLGGIFVEILEDVAFRAIPLSRHDACSMVEQIKARKILKGVRGEAAVDKSALVDLLLK
                     ISCIVDAYPQLAELDLNPVIAYDDGYAVVDARIIVNRANAS"
     gene            complement(3535..4317)
                     /locus_tag="PLD53_09455"
     CDS             complement(3535..4317)
                     /locus_tag="PLD53_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRC61183.1"
                     /translation="MQTATVLYQSNDGIAEIRLNRPQRLNAVTQQLYEELNSALGRAE
                     ADGEVRVVLLTGEGRAFCVGADLKEHKAGRTAYDRRQYLHGEQIVCKRLMQLSKPVVA
                     AVNGFALGAGAEMAIASDFVLMAASAQIGLPEISIGTFLGGGVTYLLPRLVGLAKARE
                     LVFLGERINGEEAVRIGLASRVLPDDGFLDAAREFAGKIAGKAPLSMQLAKKQLNTAA
                     ESTLDAALTAELEGMMFVGTTQDWQEGIDAFAEKRSPIFKGR"
BASE COUNT          848 a         1366 c         1359 g          924 t
ORIGIN      
        1 atggatcgac agcgcgtaac gcttgcacgt ccctcattcg ggaccggaca gaacatcggt
       61 gagcgattcg cgcgcaaact tcggtgcatc gccaaccggg acgagatcgt cgagccagca
      121 ggcgttgtca tcgcgttcgt aaacgaggaa gcgaccgcgc aggttggcat ccttggcgcg
      181 gtggtagcgc aggtagatgt gcttgtcatc gacggcgaga atctcgatct tgccggtttc
      241 gtgcgacatc gcaaaccgcg cacggcgcgc gagtcccgcg ccatggcgca gcgcctcgcg
      301 gaaaatttcc cagccgcgca cgatcggtac cttgaacggt tcgttgccgg cgatcggacg
      361 tccctggaac agatagtaag gtgggcagcc gatccacgac agcttgcgga acagttccga
      421 gagcacctcc ggatcgtcgt tgatgccggc gaccaacggg cactggttgg tcaatatgac
      481 cccggcgcgg atcagcttgt cgaggcattc gattaccggg tcggtcaatt cgcgcggatg
      541 atcgaagtgg ttcatgaagt agatgcgctt gtacggcgtc gaatgcgtcg atagcagctc
      601 gagcagtggt gcatcgtcga gtatgcgcca cgggttgaag gccgtcatct tggtaccgag
      661 gcggatgatc tggacgtggt cgatcgcacg tatcgactcg atcatgtcgg tcagccggcc
      721 tgtgctgagc aggagcggat cgccgccggt gagcagcacg ttgctgatct cagggtgctg
      781 gcgaacatag tcgagcccgg gaccgatatc cttgacggcc tcttcgttgt cgttcatgaa
      841 caggcgcttg cggaagcagt agcggcaata ggaaccgcac gcctcgttgc acaggagcag
      901 cgcggtatcc gggtacttgt gctgaacccc ctgcacaacg gtatttgccg cctcgttgct
      961 ggcgtcgaga tgaccccagt tggtgagctc cgcgatattg gggatgacca gccggcgaat
     1021 cgggtcggcc ggatcgttcc agtcgatcag gcccaggtaa tactcgctga cacggaacga
     1081 atgggtgtcg gcaacggctt tcagcttctc gcgctcggcc ttggggatct gttcgatctg
     1141 ctcaatattc gtgatgtact tcatgctctg ctcctgaatg cgctgtatcg gtaatttggc
     1201 ggcgatccag cgctgaatcg cgctggtcgc gtgcgtgttg ttcgggcggc aatgccgttg
     1261 cctggatcga catacttcca ggcgtggtct gcccgtgaag ggcaagtaag agaaaaaccg
     1321 gacttcggac tgctggcgtt gaagcttgtt ctagcgatag ctagcttgcg ttcgcacggt
     1381 tgacgatgat ccgggcatcc actacggcgt atccatcgtc gtaggcgatc accgggttga
     1441 ggtccaattc cgccagctgc ggataggcat caacaataca cgaaattttc agcaacagat
     1501 caaccagtgc cgatttgtcc accgcagctt cgccgcgcac gcccttgaga atctttcgtg
     1561 ccttgatctg ctcgaccatc gagcaggcgt cgtggcgcga cagcggaatc gcccggaaag
     1621 ccacgtcctc aaggatttcg acgaagatgc cgccgaggcc gaacatcagc accgggccga
     1681 agatcgggtc gcgcatgacg ccaatgatca cctcggtacc cttgcgcgcc atcggtgtca
     1741 ccagcattcc ctcgataacg gcgtccgggc tatagtcgcg gcaggattcc ataatgctgc
     1801 cgtaggcttc gcgcagtgcg gtttcgccgc gcaggttgag cttgacgcca cccgcatcgg
     1861 atttgtgcag gatatccttt gaaaccactt tcatgaccag cggctggctg ccgaaatgtg
     1921 cggcgacttc cgtcagtttg tcggcctctc gcaccaccat ttccgccggc atggcgacgc
     1981 cgtgtgtgcg caacagcgac ttcgcctcgt attcgaacag atcgcggccc tctttcagag
     2041 ctttcgcaaa tgtcgcttgc acctcaacgt ccggcgtcac cgcggcgagg tgtgtatgtt
     2101 cagcgctggc agcgagatag attccgcgct cgcccagtgc cgccagtacg cgaaccgcat
     2161 gttcgatcga gttatagacg ggcagtccgg cctcgtgcag ccggcgcagt gccgggggac
     2221 tgatcggcgc gtacaggctg tagatcacca atggtttgtc ggttttgtgc gccagttcga
     2281 tcatcgattc ggcaccacgc atctcgtcac ccagcaggtg ctccgcgaaa cggatgtgat
     2341 agccaccgaa catgccgacc aggaatatgc tgtcgacgtt gtcgtcttcg ttaaggattt
     2401 ccatgcattc ggcgagcagc gacggacgaa cgtcggtgct gcccgccacg tcgaccggat
     2461 tgaccagcga ggcctgcggc agaagaattt cgcccaggcg atggcgcgtg gcgtcgctga
     2521 gcaccgaaag ctccaatccg gcttccgtca gccggtcgga tgcgatcgtt gcctggccgc
     2581 caccatccga aatcaccgca acgcgtttgc cgcgtgcttg ctgcaacaaa cccagacctt
     2641 cggccaccgg caggatttcg tccgagtgct gcacgacact gacgccgacc tgacgcaaga
     2701 cgtcgaccgt cattgcgtag ctgccggcca gtgcgccggt atgcgaactg gccgccttgc
     2761 gaccctgctc ggtggagccg gacttgtaca ccaccaccgg cttggttgcc gtgacttcac
     2821 gcgcaacgtt gaggaagcgg cgtccgtcac ggaaaccctc gacgtagaac gtggcgacgc
     2881 gggtgttcgg gtcttcgctc agatagcgca gatagtcgtt gaaaccgatg tcggtctgat
     2941 tgcctggtcc gacataggtg ctgaagccga catggccgtt gtgttctgct tccagcacca
     3001 gcgacagcag catgttgccc gactgcgaaa tgaagccgat atcgccggcc ttgacattgg
     3061 ccaggtcaag cagattgagc ttcttgtgca gattgaacat tcccgacgta ttgggaccga
     3121 tcacgcgcac gccgccctga cgcgcggcat ccaataactg ttgctccagt ttggcgcctt
     3181 ccggcccggt ttcgcgaaag ccgctggcca gaatcaccgc tcccttgacg ccctttttgc
     3241 cgcattcgac cagcagcgcg ggcgccatgg ctgcgggcgt gcagatcagc gccagatcgg
     3301 gtgaggcggg caagtcggcc agggacggat aggctttgac gccgagaatc atctttgcct
     3361 tgggattgac cgggtagatc gtgccctggt agccatcctt gaccagcccg accatggctt
     3421 tgtagccgcg cttggtcggg tcggcggaag cgccgacgat ggcgatcgaa tccggagcga
     3481 ggaagtcgtg cagggggctg cgctgcggtg atgcggtttc tgtactgctc atgtctagcg
     3541 tcctttgaat atcggtgagc gtttttcggc gaaggcatcg atgccttcct gccagtcctg
     3601 tgttgtgccg acgaacatca ttccctccag ctcagccgtc agtgcggcat caagcgtgct
     3661 ttcggcggcc gtgttcaatt gcttcttggc gagctgcatc gagagcggcg ccttgccggc
     3721 aatctttccc gcgaattcac gtgccgcgtc gagaaagccg tcgtccggca gcacccgact
     3781 ggccagcccg atgcgtaccg cttcttcgcc attgatgcgt tcgccgagaa acaccagttc
     3841 gcgcgccttg gccaagccga ccaggcgcgg cagcagataa gtgacaccgc cgccaaggaa
     3901 tgtcccgatg ctgatctccg gcaatccgat ctgcgcgctc gcagccatca agacaaaatc
     3961 cgaggcaatc gccatttcgg caccagcccc aagggcaaag ccattgactg cagcaactac
     4021 cggcttcgat aactgcatca gccgtttgca gacgatttgc tccccgtgca gatattggcg
     4081 ccgatcgtac gccgtgcgcc ctgccttgtg ttccttgagg tcggcaccaa cgcagaaggc
     4141 ccggccttca cccgttagca gcaccacgcg cacctcgccg tcggcctcgg cccggcccaa
     4201 tgccgaattc aattcctcat agagctgctg ggttaccgca ttaagccgct gcgggcgatt
     4261 gaggcggatt tccgctatcc cgtcgttcga ttgatagagg acagttgcgg tttgcatgac
     4321 aggcgctccc tttagacttc aacagaagaa tgctaccagc ttatgccggt accgcctaag
     4381 cgcggagcct gatcgtgagc ggatgcccgg taattgactc agccctgtat ccccggtgca
     4441 gccgacaacc gcgcgggagg aggacagctt gcccacatcc accatcccgc ccaaacg
//
LOCUS       DAORPM010000201         4487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5824_length_4487_cov_7.780912, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000201 DAORPM010000000
VERSION     DAORPM010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5824_length_4487_cov_7.780912"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLD53_09460"
     CDS             <1..591
                     /locus_tag="PLD53_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRC61184.1"
                     /translation="ATQNLLAGTGRLAGAFILLFMMGAGLAIGTQISQHLFPAHTTGT
                     FAPVPPWVIWPAIAALGASMLGLLQAPLASVHAMIGGSLLAWTVFSLVGAVLGNVVGA
                     FVGALAVASAGHLYTYLTGQSNLLVKIPGLITLVPGSMGFRGLHALIEKDNVVGVSLI
                     TDMVLTGAVLAVGLLLADNIAPLLFTRRDVRKNTVR"
     gene            679..1611
                     /locus_tag="PLD53_09465"
     CDS             679..1611
                     /locus_tag="PLD53_09465"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRC61185.1"
                     /translation="MNTTLLPNSNIWIAYGGVLLFLVAGSGITRYLVNAFPDRDYTNL
                     RLRVRTWWMIVPALIGALTIGPTAIVLLFAIASAVTLHEYLVLTGSSHQPVPVLAAIY
                     AATPILYALAYYADNVSIGLFIPLYCLLLVSVIFAVRRGAGVWRTSATVLLGVFLTVF
                     CLSYIARLVTLSGGNGLVLFLLMITQLNDVSQYISGKMFGRTPLAPTLSPNKTREGML
                     GGLATTAVIASFLGPLLTPLSSLIAVIYGTALALAGVAGDLTVSAIKRMAGVKDAGKL
                     LPGHGGFFDRVDSLIYTAPLFFYGILVWQEMQPY"
     gene            1665..2321
                     /locus_tag="PLD53_09470"
     CDS             1665..2321
                     /locus_tag="PLD53_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRC61186.1"
                     /translation="MDCFLRRITIISSMRTFVETRFVWRKFLPVLAIYAGLLAGTVYA
                     DDNGHEEDRKQLRALMNEVEAGINAQDVDRLTAMMADNVTVTWLNAEVSRGKSEVKAY
                     YSRMVGGEGAILKKYETKVSLSAPAHFYGDVAVADGKADDAFYPHKRDLFHLDSRWSS
                     TLGKIDGNWRIITLHLSANVFTNPLMAEAERWIWFAGVGGVLLGSLAVFTIGYLRRRQ
                     "
     gene            2272..2997
                     /locus_tag="PLD53_09475"
     CDS             2272..2997
                     /locus_tag="PLD53_09475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRC61187.1"
                     /translation="MAVWPYLLSVTCDDANDVFTATRACCVQSCRVASRRKLDTKPMI
                     STYALKQRFQSYLRPLVDRLAQTNISANTVTLCTMTASCLLGLLLFFSEQETLFLLLP
                     LWMFVRMALNAIDGMLAQEHGQKSRMGAYLNELTDVISDAALYVPFVAVPPFGWPSVG
                     AVIFLSTLCEFAGALGLSIGTCRRYDGPMGKSDRAVVFGVLGLVYGMAGQLPSWAFWI
                     MPAMAILLLITAFNRVRSGLADL"
     gene            3021..>4487
                     /locus_tag="PLD53_09480"
     CDS             3021..>4487
                     /locus_tag="PLD53_09480"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HRC61188.1"
                     /translation="MSAPRPSLEASFTTHDGVPLFYQHWPAERTPRRGAIVIFHRGHE
                     HSGRMAHLASELDLPDFDFFAWDARGHGRSPGVRGFSPSLACSVRDVNTFVRHIESSH
                     GVATDDMVILGQSIGAVLVGVWAHDYATPVRCLVLASPAFKVKLYVPFAVRGLSLAHR
                     LLGNFFVSSYVKSRFLTRDVQRQESYDADPLISRSISVNMLLDLYRTAPRIIADAQAI
                     TAPTLLLISEKDWVVHHAPQHEFFSRLGSSIKEKHVLTGFLHDTFGEYDRRAAINTTR
                     EFVLRQFAAPLYRPDLRAADQHGAMHDEAVALSRPLSLLSPRGLYWSLSRLNIRFGSL
                     WSAGLRLGRQTGFDSGSSLDYVYRNKPTGHGIVGRLIDKIYLNAIGWRGIRQRKQHIM
                     ELLCLAMDNLYRDRQAVRILDIAAGHGRYIFDAFQHYPRRPESIRLRDFDNMNVKDGT
                     ALIRASGFEDIAQFVAGDAFDAADLAATAPPASVGIVSG"
BASE COUNT          873 a         1291 c         1226 g         1097 t
ORIGIN      
        1 gcgacacaaa acctgctcgc cggcaccggg cggctggccg gggccttcat cctgctgttc
       61 atgatgggcg ccgggctggc gatcggcacg caaatcagcc agcacctctt cccggcgcac
      121 actaccggaa ccttcgcccc ggtaccgccc tgggtcatct ggccggccat tgccgcgctc
      181 ggagcctcga tgctcggtct cttgcaggcg ccgctcgctt ccgttcatgc gatgattggc
      241 ggcagtctgc tggcctggac ggtgttttcg ctggtcggcg ccgttctcgg caacgtcgtc
      301 ggcgccttcg tcggcgcgct cgccgtggcc agcgcgggcc acctctatac ctatctcacg
      361 ggccagtcca acctcttggt aaaaatcccc ggcctgatca ctctggtccc cggcagcatg
      421 ggatttcgcg gcctgcacgc cctgattgaa aaagacaacg tcgttggcgt cagcctgatc
      481 accgacatgg tccttacggg tgccgtgctg gctgtgggat tgctgctggc cgacaacatc
      541 gcgcccttgc tgttcacccg acgcgatgtc cggaagaata cggtgcgctg aatccggcag
      601 tcacgtgcaa aaggcgcccc acatgagtcg gagagtgact tgccaaatca accgagtcgt
      661 gacggataat gcgcggccat gaataccact ttgcttccaa actcgaatat ctggattgcg
      721 tacggcggcg tcctgctgtt tcttgtagct ggcagtggca taacccgtta tctggtgaac
      781 gcttttcctg atcgtgatta cacgaacttg cgtctgcgag tgcgtacatg gtggatgata
      841 gtgcccgcat tgataggagc gctcacgata gggccaaccg ccatagtgct tctattcgcg
      901 atagccagtg ctgtcaccct ccatgaatat ctcgtactca ccgggagcag ccatcagcct
      961 gttccggttc tagctgccat ctacgcggca acaccgatcc tttatgcatt ggcctactac
     1021 gccgacaatg taagcatcgg tttattcatt ccgctctatt gcctgctgct tgtcagcgtg
     1081 atctttgctg tccgtcgcgg cgcaggcgtt tggcgtacca gcgctacggt tcttctgggc
     1141 gtctttttga ctgtcttttg cctcagctac attgctcgac ttgtcacgct ctccggtggg
     1201 aacggcctgg tactctttct cttgatgatc actcaattga atgatgtatc gcaatatata
     1261 tcgggaaaga tgtttggtcg cacaccactc gcgcctacac tgagtccaaa caagacgcgg
     1321 gaaggtatgc tcggcgggct ggccactaca gcggtaatcg catcatttct tggtcccttg
     1381 ctaactccat tgagcagcct gattgctgtc atttatggca ctgcccttgc gttggctggc
     1441 gttgcaggtg acctgacagt ttctgcgatc aagcgcatgg caggggttaa ggacgcaggc
     1501 aaattattgc ccggccacgg cggattcttc gatcgcgtcg atagcctgat ctatacagca
     1561 ccgctgttct tttatggcat cctggtttgg caggagatgc aaccatactg aaggtttttg
     1621 cgccgtgcca ccccttgtcg tattccattt ttttcggagt catcatggac tgttttcttc
     1681 gccgtatcac tattatttct agcatgcgca ctttcgtcga aacacgattt gtatggcgca
     1741 aattcttgcc tgtcttggcc atttatgcgg ggctactggc aggaaccgtt tatgctgacg
     1801 acaatgggca tgaggaagac cgcaagcaac tgcgcgcctt gatgaacgaa gttgaagccg
     1861 gcattaacgc gcaagatgtc gaccgattaa cggcgatgat ggcagacaac gtaacagtga
     1921 cttggctgaa tgcggaagtt tcgcgtggaa aaagcgaagt gaaagcgtac tactcgcgaa
     1981 tggttggggg cgagggcgca attctgaaga agtatgaaac caaagtatca ctgagcgcgc
     2041 cagcgcactt ttacggtgac gttgccgtag ctgacgggaa agccgacgat gcgttttacc
     2101 cccacaaacg cgacctgttc cacctcgatt ctcgatggag ttcaacttta ggcaaaatcg
     2161 acggcaattg gagaatcatc accttgcatc tgtcggcaaa cgtcttcacc aacccactga
     2221 tggcggaagc cgagcgctgg atctggttcg ccggcgtcgg aggcgtcctt cttggcagtc
     2281 tggccgtatt tactatcggt tacctgcgac gacgccaatg acgtcttcac agcaacccgc
     2341 gcatgctgcg ttcaatcatg ccgagttgcc tcgcgtcgca agcttgatac aaaaccaatg
     2401 atctcgacct acgcgctgaa gcagcgtttc cagtcctatc ttaggccctt ggtagatcgc
     2461 cttgcgcaaa cgaatatctc agcgaacacg gtgacgcttt gcaccatgac ggcatcttgc
     2521 ctattggggc tattgctgtt tttttccgag caggagacct tgttcctgtt gctgccattg
     2581 tggatgtttg tgcgcatggc cctcaatgca attgacggaa tgctcgctca ggagcacggc
     2641 caaaaatcgc gaatgggagc ctacctgaac gaactcaccg atgtaatctc tgatgccgct
     2701 ctgtatgtgc cctttgttgc cgttccgccg tttggctggc ccagtgtcgg cgcagtaatc
     2761 ttcctttcca ccttgtgtga attcgcaggc gcacttggct tgtcgatagg cacgtgccgc
     2821 cgctacgatg gcccgatggg aaagagtgac agggcagtgg tattcggcgt ccttggcctc
     2881 gtttatggaa tggctggtca gttgccctcg tgggctttct ggatcatgcc ggccatggcg
     2941 attttgcttc taatcacagc cttcaaccgc gttcgcagcg gattggcaga cttatagaat
     3001 ctctgaagat atcattcgat atgagtgcac ctcgtcccag tctggaagcc agtttcacaa
     3061 cgcatgatgg cgttcccctg ttctatcaac actggccggc cgagcgtacg ccgcgtcgcg
     3121 gtgcaatagt catcttccac cgggggcatg aacattccgg ccgcatggca catctggcca
     3181 gcgaactgga tctcccggat ttcgacttct ttgcctggga tgcacgtggc catggtcgct
     3241 caccgggagt tcgtggcttt tcgccaagcc ttgcatgttc agtacgtgac gtgaatactt
     3301 ttgtcaggca catcgaatcg agccacggtg tggccacgga tgacatggtg attctcggcc
     3361 aaagcatagg cgctgtgctg gtcggcgtct gggcgcatga ctacgccaca ccggtccgtt
     3421 gtctggtgct tgcctcgccg gccttcaagg tcaagcttta tgttccgttt gccgtacgcg
     3481 gcctgagtct cgcccatcgc ctattgggca atttctttgt cagcagttat gtcaagtcca
     3541 ggtttttgac gcgcgacgtg caacgtcaag aatcgtacga tgcagaccct ctgatcagtc
     3601 gctccatttc cgtaaacatg ttgctggatc tatacaggac tgctccacgc atcatcgccg
     3661 acgcgcaagc cattacggca cccacattgc tgctgatatc ggagaaagat tgggtcgtgc
     3721 atcacgcccc gcaacatgaa ttcttttccc gcctggggag ctcgataaag gaaaagcatg
     3781 tcctcaccgg atttctgcat gacaccttcg gcgaatatga ccggcgagca gccataaaca
     3841 caacccggga gttcgttctc cgccagttcg cggcgccatt gtatcgcccg gatcttcgcg
     3901 cggctgacca gcatggagcc atgcacgacg aagcggtagc cctttcacga ccgctttcac
     3961 tactgtcgcc ccgcggcctc tactggagtc tgtcacgctt gaatatcagg tttggaagct
     4021 tgtggtcagc cgggcttcgc ctcggccgtc aaacgggctt cgattccgga agctcgcttg
     4081 actatgtcta tcgcaacaag ccgacggggc acggcattgt tggccggctg atcgacaaga
     4141 tctatctgaa tgccatcggc tggcgcggca ttcgccagcg caaacaacac atcatggagt
     4201 tgttgtgtct ggcgatggac aatttgtatc gggatcggca agccgtcaga atccttgaca
     4261 tcgctgccgg acacggccgc tacattttcg atgcctttca gcactatcca cgacgtccgg
     4321 agtcgattcg cttgcgcgac ttcgacaaca tgaacgtaaa ggatggcacg gccttgatca
     4381 gagcgagtgg cttcgaagac atcgcccagt tcgtcgctgg tgacgcattt gatgccgctg
     4441 acctcgcggc gacggcgccc ccagccagtg tcggcatcgt ttccggc
//
LOCUS       DAORPM010000202         4485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5833_length_4485_cov_8.483070, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000202 DAORPM010000000
VERSION     DAORPM010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5833_length_4485_cov_8.483070"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PLD53_09485"
     CDS             <1..842
                     /locus_tag="PLD53_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HRC61189.1"
                     /translation="EFPGGKVEPGESLREALVRELNEELGIIVDRAWPWLSCEFSYPH
                     ATVHLKFFRVFSWHGEIAPIEHSGFVWMKIGATLPVAPILPANGPILRALALPSTYAI
                     TNATANGIDAELARLECALTDGLRLIQVRDKTLPSSTRLQLAKGVVTLASRHADARVL
                     INDDEALAHEVGASGLHLSAGRLKQIKRRPSFAWVAASCHSAEELQRATTLGLDFVVL
                     GPVLPTATHPEATNIGWGEFARLIEQSPLPVFALGGMRTDMLEMAWEFGAHGIAQMRG
                     WGQ"
     gene            complement(853..1047)
                     /locus_tag="PLD53_09490"
     CDS             complement(853..1047)
                     /locus_tag="PLD53_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HRC61190.1"
                     /translation="MSRDRLKVSCPQCGGETVWSADNRFRPFCCERCKMIDLGAWASE
                     SYRVPVQETADPVDEPPPAE"
     gene            complement(1047..1802)
                     /gene="zapD"
                     /locus_tag="PLD53_09495"
     CDS             complement(1047..1802)
                     /gene="zapD"
                     /locus_tag="PLD53_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HRC61191.1"
                     /translation="MITYEYPFNERIRTLLRLEDLFDKASYFVEQEGTREHHVVLLTL
                     FELLEVAGRADLKMDLIQELERQRQTLLVFRNNPEISEEALSGALYEIEQAATALLGM
                     TGKMGQYLRDNDWLMGIKSRAVIPGGVCQFDLPTYHYWMHQDSAVRREALLSWLKPFM
                     PLRNGLAIVLRLLRGSGRMEHQVAERGAFQMMLGGGGAQMVRVSLELHEPYVPEISAN
                     KYALNIRFTCPDYDHRPRHAESDVDFDIVFCNL"
     gene            complement(1912..2523)
                     /gene="coaE"
                     /locus_tag="PLD53_09500"
     CDS             complement(1912..2523)
                     /gene="coaE"
                     /locus_tag="PLD53_09500"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289434.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRC61192.1"
                     /translation="MSLIVGLTGGIGSGKTTVADYFASLGAAVVDTDVIAHELTVAQG
                     AAMPEIEAAFGDSVLLADGGLDRAAMRRLVFSDPSEKCRLEAILHPMIRAASEARCQS
                     AMAARAPYVMLVVPLLLESGAYRERVDRILVVDCDEDVQIARVTARSGLSIEEVRAIM
                     ATQASRPERLAAANDVVLNSAGTEQLFAQVVALHRHYLELTVL"
     gene            complement(2520..3221)
                     /locus_tag="PLD53_09505"
     CDS             complement(2520..3221)
                     /locus_tag="PLD53_09505"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRC61193.1"
                     /translation="MRILVFLLILVNLLFLAWTQGLLGSSSNPDALRVQQQLLADQVK
                     VVSRDEPPEVITPPEKPKKVVEVKAVDICLELSDLPIAEVAQVEGMLAEKLPVFKSER
                     TAISGTSSYWVIIPPLANKGEVDTKVAELKQLDISEFFVVQESGPYNRAISLGLYTSN
                     AAATSQLERLRKKGVRSARIIERVVKPTTASLVIRGPAAQADSLSQAILGVLPESKPV
                     VCKAQSSAAAGVTAE"
     gene            complement(3239..>4485)
                     /locus_tag="PLD53_09510"
     CDS             complement(3239..>4485)
                     /locus_tag="PLD53_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRC61194.1"
                     /translation="PEQMGAIFPTAILEQEMSSERWIPIPQPVREIYRLWRPSPLVRA
                     ARLEEMLGTPAKIYYKNESVSPAGSHKPNTAVAQAYYNRQAGIKRITTETGAGQWGCS
                     MALAGQMFGLDVRVYMVKVSYQQKPYRRSMMQTWGAEVFASPSNLTQTGREALSRDPD
                     NMGSLGLAISEAVEEAASRADTNYALGSVLNHVALHQTIIGLETRKQLELAGDWPDVV
                     FAPCGGGSSFAGLAFPFIADNASGGRNGKSVRLVAVEPTSCPSLTKGDYAYDFGDASG
                     FTPLMKMYTLGHDFMPPGIHAGGLRYHGASPLVSQLYHEGLIEAMGVPQLATFEAGVM
                     FARAEGIIPAPESNHAIRAAIDEALRCKESGEAKTILFNLTGHGHFDMASYDRYFSGE
                     LEDFSYPEAAIKESLQHLPRFG"
BASE COUNT          970 a         1358 c         1261 g          896 t
ORIGIN      
        1 gggaatttcc cggcggcaag gtcgagccgg gtgagagctt gcgcgaagcg ctggttcgcg
       61 aactgaatga agagctgggt atcatcgttg atcgtgcgtg gccatggttg tcctgtgagt
      121 tcagctatcc gcatgccacc gtccacctga aattcttccg cgtcttttcg tggcatggcg
      181 aaatcgcgcc aatcgagcat agcggttttg tctggatgaa gattggcgcc acactgcccg
      241 ttgctccgat tctgcccgcc aacggcccga ttctgcgtgc gctagcattg ccgtcaacct
      301 acgccattac caacgcgacc gcgaacggca tcgatgcgga attggcaaga ctggaatgcg
      361 ctctgacaga cggactgcga ctgatccagg tacgcgacaa gacgctgccg tcctctacgc
      421 gtttgcaact ggccaagggc gtggtgacgc tggccagccg ccatgccgat gctcgcgttt
      481 tgatcaacga tgacgaggca ctggcgcacg aagtaggtgc cagcggcctg catctctccg
      541 ccggtcggct caagcagatc aagcggcgcc cgtcattcgc ctgggttgcc gcatcctgcc
      601 attcggcgga ggaactccaa cgtgcgacga cactcggtct tgattttgtc gtgctgggcc
      661 cggtcttgcc aacagcaact caccctgagg ccacgaacat aggctggggc gaattcgcac
      721 gtctgatcga gcaatcaccc ctgccggtgt ttgcgctcgg cgggatgcgg acggacatgc
      781 ttgaaatggc ctgggaattc ggtgctcacg gcatcgcgca gatgcgtggc tgggggcagt
      841 gagcgcggtg aatcactccg ccggcggagg ctcgtcaacc gggtcggccg tttcctgcac
      901 aggtacccgg tacgattccg acgcccacgc accgagatca atcatcttgc aacgctcaca
      961 gcagaacggg cgaaaccggt tgtcggcgct ccataccgtc tcgccgccgc attgcgggca
     1021 actcaccttg agccgatccc tggacatcaa agattacaaa aaacgatatc gaaatcgaca
     1081 tcgctctccg catgccgtgg gcggtgatcg taatcgggac aagtaaaacg gatattcagg
     1141 gcgtatttgt tggcgctaat ttcaggcaca tacggctcgt gcagttcgag tgacacccgc
     1201 accatctggg cgccgccacc gcccagcatc atctggaaag cgccacgctc tgccacctga
     1261 tgctccatgc gcccactgcc acgcaacagc ctgagcacaa tagccagccc gttgcgcagc
     1321 ggcatgaagg gcttgagcca gctaagcaaa gcctcgcggc gcacagcgga atcctgatgc
     1381 atccagtagt ggtaggtggg caaatcgaat tggcaaacac cgcccggaat gacggcgcgg
     1441 ctcttgatgc ccatcagcca gtcgttgtca cgcaaatact gacccatctt gccggtcatg
     1501 ccgagcagtg ccgtagcggc ctgctcgatt tcgtagagcg ccccggacag cgcttcttct
     1561 gaaatttccg gattattgcg aaaaacgagt aaggtctgac gttgacgctc aagttcctga
     1621 atcagatcca tcttgaggtc ggcacggcca gcgacttcga gaagctcgaa cagcgtcagc
     1681 aggacaacgt ggtgctcacg tgtaccttcc tgctcgacaa agtagctggc cttgtcaaac
     1741 aaatcctcaa ggcgcaatag tgtgcggatg cgctcattga aaggatattc gtatgtaatc
     1801 actaggccgc cagatgaaaa aatgagagaa aaacgcattc attctgaggc atttgcaaca
     1861 aaatctcaac ggtccgcatt aactgtctac gctaacttgg tatgcatatt gtcataaaac
     1921 tgtaagctca agataatgtc gatgcaaggc aacaacctgt gcaaacaatt gctccgttcc
     1981 tgccgaattc aaaacaacgt cattcgccgc cgccagtcgt tcgggccgtg atgcctgcgt
     2041 ggccatgatc gcccgaacct cctcgatcga gaggccgctg cgggccgtga cgcgcgcaat
     2101 ctgcacatcc tcgtcgcaat caacgaccag aatccgatcg acccgctcgc gataagcgcc
     2161 ggactcaaga agcagcggga cgaccagcat cacataaggc gccctcgccg ccattgcaga
     2221 ttggcaacgc gcttcgctcg cggcgcgaat catcggatga agaatggctt ccagccggca
     2281 tttttccgac gggtcggaaa aaacaagtcg gcgcatcgct gcacggtcca gaccaccgtc
     2341 cgcgagcagt accgagtcac cgaatgctgc ttcgatttcc ggcatggcgg cgccctgcgc
     2401 gacggtaagc tcgtgcgcga tgacgtcggt gtccaccact gcagcaccga gagaagcaaa
     2461 gtagtccgca accgtcgtct tgccactacc aatcccgccg gtcaggccaa cgatcaaact
     2521 cattccgcag tcacacccgc agccgcactt gactgcgcct tgcaaaccac cggcttgctc
     2581 tcaggcagca ccccaagaat ggcctggctc agcgaatcgg cctgtgcggc aggtcctcga
     2641 attacaagcg aagctgtcgt cggttttacg acacgctcaa taatcctggc cgatcggacg
     2701 ccctttttgc gcagcctttc gagctgcgaa gtcgccgccg cattgctggt atacaggccc
     2761 agcgatatgg cccggttgta cggcccgctc tcctgcacga cgaagaactc ggaaatgtcg
     2821 agctgtttca attcggcgac ttttgtgtcc acttcccctt tattggccaa tggaggaatg
     2881 atgacccaat agctcgaagt gccggaaatc gcagtgcgct ccgacttgaa aacgggcaat
     2941 ttctcggcga gcattccttc aacctgtgcg acttccgcga tcggcaaatc gcttaactca
     3001 aggcagatat cgacggcctt gacttctacg actttcttgg gtttttcagg cggggtgatt
     3061 acctctggcg gttcatcgcg cgataccact ttcacctgat cggccaatag ctgctgctgc
     3121 actcgcaacg cgtcgggatt ggaagacgag cccagcaagc cttgggtcca cgcgagaaac
     3181 aacaagttga ccaggatcaa aaggaaaaca aggatgcgca ttcggatttt cctcagggtc
     3241 agccaaatct tggcaaatgc tgcaaggatt ccttgatcgc ggcttcaggg tagctgaagt
     3301 cctccagttc acctgaaaaa tagcgatcat acgatgccat gtcgaagtgg ccatgtccgg
     3361 tcaagttgaa gagtatggtc ttggcctcgc cactttcctt gcagcgcaaa gcctcgtcga
     3421 tggcggcacg gatggcatga ttcgactccg gcgccggaat gatgccttcg gcgcgggcaa
     3481 acatgactcc agcttcaaag gtagccagct gcggcacgcc catcgcctca atcagacctt
     3541 cgtgatagag ctgtgaaacg agcggcgaag caccgtgata gcgcagaccg ccggcatgga
     3601 ttcccggtgg catgaagtcg tgcccgagcg tgtacatctt catcagcggg gtaaatccgg
     3661 aagcgtcgcc gaagtcatac gcgtagtcac ccttggtcaa cgacgggcag gacgtcggct
     3721 cgaccgcgac gagtcgcacc gactttccgt tccgtccgcc gctcgcatta tcggcgatga
     3781 acgggaaagc cagaccggca aacgacgacc cgccaccaca aggggcaaag acgacatccg
     3841 gccaatcacc ggcgagttcg agttgtttcc gggtttccag accgatgatc gtctggtgca
     3901 gcgccacatg gttgagtacc gagccgaggg catagttggt atcggcgcgc gaagcggctt
     3961 cctccaccgc ctcggaaatc gccagaccga gcgagcccat gttatccgga tccctgctga
     4021 gtgcttcgcg tccggtctgc gtcaggttcg acgggctggc gaacacctcg gcgccccagg
     4081 tttgcatcat cgagcggcga tagggcttct gctggtaact gaccttgacc atgtagaccc
     4141 ggacgtcgag cccgaacatc tggccagcca gggccatcga gcaaccccac tgcccggcgc
     4201 cagtttcggt agtgatgcgc ttgatgccgg cctgccggtt gtaataggcc tgcgccacag
     4261 cggtattcgg tttgtgcgaa ccggccggcg aaacgctttc gttcttgtag taaatctttg
     4321 ccggagtacc cagcatctct tccagcctcg cagctcgaac gagtggcgac ggacgccaca
     4381 aacgataaat ctcgcgcacc ggttgcggga tcgggatcca gcgctcgctc gacatttcct
     4441 gttcgagaat cgccgtcggg aaaatggcac ccatctgctc tggcg
//
LOCUS       DAORPM010000203         4431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_5967_length_4431_cov_10.782450, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000203 DAORPM010000000
VERSION     DAORPM010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5967_length_4431_cov_10.782450"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2246
                     /locus_tag="PLD53_09515"
     CDS             <1..2246
                     /locus_tag="PLD53_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC61195.1"
                     /translation="DKAQKLIGKLVFATAFVVLLVFFALGRREALIVGLAVTLTLAAT
                     LFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRWQGLHPGKPLLEIIPQAVD
                     EVGGPTILATLTVIAALLPMAFVTGLMGPYMSPIPINSSMGMLISLVVAFVVTPWLAA
                     RLMQGHATSTTETQRHGENTEKNRFIALLFSAAPQRLSASALDSFFRRLLTPFLAPAT
                     GKSARRKLLLGIILLILGSVSLAGFKLVVLKMLPFDNKSEFQIVLDMPVGTPVEETAR
                     VLHEIGAELAKVPEVVNYQAYAGTASPINFNGLVRQYYLRAAPESGDLQVNLADKKHR
                     SRQSHEIAISVRDRVEEIGRKNGGNAKVVEVPPGPPVLSPIVAEVYGPDYQGQIAVAK
                     EVRKVLEKTPDLVAIDDSVQADARKTVLHVLQAKAALLGVAQSDIVEVVRMGLAGEDV
                     TPVHNADSKFEVPVRVTLPPEKQSSLDALLKLRVRSREGMLVPVSELVEVRDMAREKN
                     IYHKDLLPVVYVYADMGGKLDSPLYGMFDARGELAGKSLIGVEKAGGTLGEWFIRQPG
                     DPYAGYSIKWDGEWQVTYETFRDMGTAYAVGLILIYLLVVAHFKSYLVPLIIMAPIPL
                     TIIGVMPGHALLGSQYTATSMIGMIALAGIIVRNSILLVDFIRQQADAGMAFSEAIIQ
                     SAAVRAKPIALTGLAAMLGAMFILDDPIFNGLAISLIFGIFVSTVLTLIVIPVLYFAA
                     FNKEYSS"
     gene            2243..2497
                     /locus_tag="PLD53_09520"
     CDS             2243..2497
                     /locus_tag="PLD53_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRC61196.1"
                     /translation="MKTPFTTDRAIRIMAGSFILLSLALGVEASPLFVSKNFLWFTAF
                     VGANLFQSGFTRFCPAESLMVWLGMKRCDGVNYNESKLVR"
     gene            2555..3271
                     /gene="pgl"
                     /locus_tag="PLD53_09525"
     CDS             2555..3271
                     /gene="pgl"
                     /locus_tag="PLD53_09525"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRC61197.1"
                     /translation="MKRNDAQRCRWHAVSDQHALRTAALETILASAAQSIQERGTFHL
                     VLAGGNTPREIYRGLSTAQADWAAWHIYFGDERCLPPDDPERNSYMARKAWLDGVPIP
                     PLQVHAIAGELGAVRASRQYSELLRPVGDFDLVLLGLGEDGHTASLFPDHEWGAEAGS
                     PDALAVFDAPKPPSERVSMSAARLSRARQVLFLVSGESKRLAVSTWRAGSNIPARAIQ
                     PASGVDILIEAGLLETLSAM"
     gene            3310..4329
                     /gene="gnd"
                     /locus_tag="PLD53_09530"
     CDS             3310..4329
                     /gene="gnd"
                     /locus_tag="PLD53_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HRC61198.1"
                     /translation="MELGMIGLGRMGANLVRRLTKEGHVCVVYDVDPEAVKQTEGPSI
                     QGAASIEALLGKLTKPRVVWIMVPAAVTGETVDKLASLMEAGDIIIDGGNSYYRDDLA
                     RAEALKPTGIHYVDCGTSGGVFGLERGYCLMIGGEKQIVRYLDPIFRSIAPGVGAAPR
                     TPGREGTVGQAEHGYLHCGPVGAGHFVKMVHNGIEYGLMAAYAEGMNILRHANAGKRL
                     RAADAETAPLRDAQFYQYDLDLPQITEVWRRGSVIGSWLLDLTAASLVESPDLQDFAG
                     RVSDSGEGRWTVQAAIDEAVPAPVLSAALFERFSSRDGADFANRVLSAMRKQFGGHDE
                     KKGNH"
BASE COUNT          812 a         1320 c         1409 g          890 t
ORIGIN      
        1 ccgacaaggc gcagaagctg atcggcaagc tggttttcgc gacggccttc gtcgtcctgc
       61 tggtgttctt tgcgctcggc aggcgcgagg cactgatcgt cggcctggcg gtaacgctga
      121 cccttgccgc aacgctcttc gcttcgtggg cctggggttt cacgcttaat cgcgtttcgc
      181 tctttgccct gatcttctcg attggaattt tggtcgacga tgccattgtc gtggttgaga
      241 acatccatcg ctggcagggg ctgcatcccg gcaaaccgct acttgaaatc attccgcagg
      301 cggtcgatga ggtcggcggc ccgaccatcc tcgccaccct gacggtgatt gccgcgctgt
      361 tgccgatggc ctttgttacg ggcctgatgg gcccgtacat gagcccgatt ccgatcaact
      421 cgtcgatggg catgctcatt tccctggtgg tggcatttgt cgtgacgccg tggctggcgg
      481 cgcgcctgat gcaggggcac gccacatcca ccacagagac acagaggcac ggagaaaaca
      541 cagagaagaa tcgttttata gccttgcttt tctctgcggc acctcagcgc ctcagtgcct
      601 ctgcgttgga ttcgttcttc cgtcgtctgc tcacaccgtt ccttgcgccg gccaccggca
      661 agtccgcacg cagaaagctg ctgctgggca tcattctgct gatcctcggc tcggtgtcgc
      721 tcgcaggctt caagctggtc gtactgaaga tgctgccttt cgacaacaag agcgaattcc
      781 agatcgttct ggatatgccg gtgggaacgc cggtcgagga aacagcgcgc gtgttgcacg
      841 agatcggcgc cgagctggcc aaagtgccgg aagtcgtcaa ctaccaggcc tacgccggca
      901 cggccagtcc gatcaatttc aacgggctgg tgcgccagta ttacctgcgc gccgcaccgg
      961 agtcgggcga tttgcaagtc aacctggccg acaagaagca ccgttcgcgc cagagccacg
     1021 agatcgcgat ctcggtacgc gaccgcgtcg aggagatcgg tcgaaaaaac ggcggcaacg
     1081 ccaaggtggt cgaagtgcca cccggcccgc cggtgctatc gccgatcgtc gccgaagttt
     1141 acgggccgga ctatcagggc cagatcgccg tcgccaagga ggtgcgcaag gttctggaga
     1201 aaacaccgga tctggttgcc atcgacgatt cggttcaggc cgatgcgcgc aagaccgtgc
     1261 tgcacgtgct tcaagccaaa gctgcattgc ttggcgtggc gcagagcgac atcgtcgaag
     1321 tcgtgcgcat ggggcttgcc ggcgaggatg taaccccggt gcataacgcc gactcgaagt
     1381 tcgaagtgcc ggtgcgggtg accctgccgc ccgagaaaca aagctcgctc gacgccctgc
     1441 tcaaactcag ggtgcgttcg cgtgaaggga tgctggtgcc ggtttccgag ctggtcgagg
     1501 tgcgcgacat ggcgcgggag aagaacatct accacaagga tctgttgccc gtcgtttacg
     1561 tctacgccga catgggcggc aagctggatt cgccgcttta cgggatgttc gatgcgcgtg
     1621 gcgaactggc cggcaagtcg ctgatcggcg tggaaaaagc cggaggtacg ctcggcgagt
     1681 ggttcatccg ccaacccggc gatccctatg cgggctacag catcaagtgg gacggcgagt
     1741 ggcaggtaac ttacgaaacg tttcgcgaca tgggcacggc ctacgccgtc ggcctgatcc
     1801 tcatctacct gctcgtggtg gcacacttca agagctatct ggtgccgctg atcatcatgg
     1861 cgccgattcc gctgaccatc atcggcgtca tgcccggcca cgcactgctc ggatcacaat
     1921 ataccgcgac atcgatgatc ggcatgatcg ctctcgccgg aatcatcgtg cgcaactcga
     1981 tcctgctggt cgacttcata cgccagcagg cagacgcggg gatggcgttc agcgaagcga
     2041 tcatccagtc cgctgccgtt cgcgccaagc cgattgcctt gaccgggctg gcggcgatgc
     2101 tgggtgcaat gttcatcctc gacgacccga tcttcaacgg gctggccatc agcctgatct
     2161 ttggcatttt cgtttccacc gtcctgacgc tgatcgtcat cccggtgctc tatttcgctg
     2221 cgttcaacaa ggagtactcg tcatgaaaac accctttacc accgaccgtg ccatccgcat
     2281 catggccggc agcttcatcc tgctttcgct cgccctgggt gtcgaagcca gcccgctctt
     2341 cgtctcgaag aacttcctgt ggttcactgc ctttgtcggt gccaacctgt tccagagcgg
     2401 cttcacgcgt ttctgtccgg ccgaatcgct catggtctgg ctgggtatga aacgctgcga
     2461 cggcgtaaac tataatgaaa gcaaactcgt tagatagaac gtattgacct gaatttgcct
     2521 cgcgtgcgag tacgtatcga ggccggatcc gccgatgaaa agaaatgacg cgcaacggtg
     2581 tcgctggcat gcggtttccg atcagcatgc gctgcgaaca gcagcgctgg aaaccattct
     2641 cgccagtgct gcacagtcca ttcaggaacg cggtacgttt catcttgtgc tggccggcgg
     2701 aaacacgccc cgcgaaatct atcgcggctt gagtacggcc caggccgact gggccgcgtg
     2761 gcacatctat ttcggcgacg agcgttgcct gccgcccgat gatcctgagc gcaattcgta
     2821 catggcccga aaggcctggc tcgatggtgt gccaattccg ccgcttcagg tgcacgccat
     2881 cgccggggag ttgggggctg tgcgggcgtc ccgacagtat tccgaattgc tgcggccggt
     2941 cggcgacttc gatctggtgc tgctcggcct gggtgaggac ggccacaccg cgagcctctt
     3001 ccccgatcat gagtgggggg ctgaggcggg gtcgccggat gcgctggctg tgttcgatgc
     3061 gcccaagccg ccatcggaac gcgtgtcgat gagtgcggcg cgcctgagcc gcgcgcgaca
     3121 ggtgctgttt ctcgtcagcg gcgaatcgaa gcgtttggcg gtgtccacgt ggcgcgccgg
     3181 cagcaatatc cccgcgcggg caatacaacc ggcgtcggga gtcgatatac tgatcgaagc
     3241 aggtttgctg gaaacattat cggccatgtg agcctgttct ctccaaccgg ccagacaagg
     3301 aggttcgcta tggaactcgg catgatcggt ttaggtcgca tgggcgccaa tctggtgcgg
     3361 cgactgacga aggaaggaca cgtatgtgtt gtctacgacg tggacccgga ggcggtaaaa
     3421 cagactgaag gccccagcat tcagggcgcc gcatcgatcg aggcgctgct cgggaaactc
     3481 accaagccgc gcgtggtctg gatcatggtc cccgccgcgg tgaccggcga gacggtcgat
     3541 aaactggctt cactcatgga ggccggtgac atcatcatcg acggcggcaa cagctactac
     3601 cgcgacgatc ttgcaagagc ggaggcgctc aagccgacgg gaatacacta tgtcgactgc
     3661 ggcaccagcg gcggggtgtt cgggctggag cgcggctact gtctgatgat cggcggagag
     3721 aaacagatcg tgaggtacct ggacccgata ttccggagca ttgcgcccgg tgttggcgcc
     3781 gcgccgcgca cgcctggccg ggagggcacg gtgggtcagg ccgaacacgg ctatctgcat
     3841 tgcgggcctg tcggcgccgg ccatttcgtc aagatggttc ataacggcat cgaatatggc
     3901 ctgatggcgg cgtatgcgga ggggatgaat attctgcgcc acgccaacgc cggcaagcgc
     3961 ttgcgcgctg cagatgctga aacggcgccg ttgcgcgatg cgcagttcta ccagtatgac
     4021 ctggacttgc cgcagatcac cgaagtctgg cgccgcggca gcgtgatcgg ctcgtggctg
     4081 ctcgacctga cggcggcttc gctggtcgaa agtcccgatc tgcaggattt tgccggccgc
     4141 gtgtcggatt ccggagaagg gcggtggact gtccaggcgg cgattgatga ggccgttcct
     4201 gcgccggtgt tgagcgccgc gctgttcgag cgcttcagct cgcgcgatgg cgcggatttc
     4261 gccaaccgcg tcttgtccgc catgcgcaag cagtttggcg ggcatgacga gaagaaagga
     4321 aatcactgac atggacggca ccaggcgcgg cgcgccggaa tcggatgcgc tggtactgtt
     4381 cggcgtgacc ggcgaccttg cgtacaagaa aatctttccg gcgctctacg c
//
LOCUS       DAORPM010000204         4396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6048_length_4396_cov_11.829072, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000204 DAORPM010000000
VERSION     DAORPM010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6048_length_4396_cov_11.829072"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..193)
                     /locus_tag="PLD53_09535"
     tRNA            complement(118..193)
                     /locus_tag="PLD53_09535"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Gly,seq:gcc)
     gene            complement(309..887)
                     /gene="pgsA"
                     /locus_tag="PLD53_09540"
     CDS             complement(309..887)
                     /gene="pgsA"
                     /locus_tag="PLD53_09540"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRC61199.1"
                     /translation="MPFSIPILLTWLRIVLIPLMIGVYYVPETWIVEVGRNLAATLIF
                     VLAGVTDWLDGYLARRWNETSAFGAFLDPVADKLVVAAALIMLVQLGRLDAVLAAIII
                     GREITISALREWMAQMGAHKSVAVSAIGKVKTTAQIVSISMLLYHDPIGNFDVQGMGT
                     WLIYVAAVLTLWSMGYYMRMAWSHLDDMDKKA"
     gene            complement(887..2713)
                     /gene="uvrC"
                     /locus_tag="PLD53_09545"
     CDS             complement(887..2713)
                     /gene="uvrC"
                     /locus_tag="PLD53_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRC61200.1"
                     /translation="MDETFDANALLETLTEFPGVYRMLDVDGRVLYVGKAKNLKKRVA
                     SYFRENHPSPRIALMVAQIASVETTVTRSEAEALLLENNLIKSLAPRYNILFRDDKSY
                     PYIVITRDRYPRLGFYRGNTDRKADYFGPFPSTTAVRESLNLLQRMFRLRTCEDSVFS
                     NRSRPCLLYQIKRCSAPCVDFVAPEIYAQDVQRASLFLQGRQQEVIGQLGEAMHQAAT
                     ELAFERAAVYRDQIQSLRQIQDKQYVESSKGEDVDIVVVVEEAGLLCVNLAMVRGGRH
                     LGDKTQFPSNVGDSSPEEVLSAFLSQHYLIHPIPMRIYLNRALPDSETVNALAELAGH
                     PVPVLEPRLTLHKVWVEMAEQNARLAIHARRNVLSRQASRLDALFECLGVEPEDSEAN
                     KQARIECFDISHTQGEATVASCVVYQGDGMRKSDYRRFNIQGIQPGDDYAAIRQVVLR
                     RYEKVSSGESVAPALILIDGGKGQVNAALSALEELGLAHLPMLGVAKGEGRKPGLEKL
                     IFADGREPLQLAAEHPALNLIQEIRDEAHRFAISGHRARRSLTRRTSRLEEIAGIGPK
                     RRKALISHFGGLQGISNAGIDQLTAVPGISRELARTIYTALH"
     gene            2784..3959
                     /gene="earP"
                     /locus_tag="PLD53_09550"
     CDS             2784..3959
                     /gene="earP"
                     /locus_tag="PLD53_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HRC61201.1"
                     /translation="MTQPAKPLEWDIFCNVIDNFGDIGVCWRLSRQLTVEHDCRVRLW
                     VNELEAFQRICPEIDPQIPIQHVSGVEVRRWASTFPESVPGDVVIEAFGCHLPEPFVD
                     AMAGCRAPPVWINLEYLSAEAWVSGCHTLPSPHPNLPLTKYFFFPGYGEGTGGLLREH
                     DLEERRQGFCTSVEQQKDFWFSLGLPPPPADALVVSLFAYENSAIGDLLTVWARGEEP
                     VCCLAPESRTLAALEEFAGQSMRAGDTIRRGKLELRVLPFVTQQDYDRLLWASDISFV
                     RGEDSFVRAQWAAKPMVWHIYPQDEGAHLSKLGAFLDLYTAGASQATAEAVRVLHNAW
                     NGDLSGERHAPRITPELWQQWMDALPDLRNLAENWANHLINQEDLCSSLVRFCRSKL"
     gene            3999..>4396
                     /locus_tag="PLD53_09555"
     CDS             3999..>4396
                     /locus_tag="PLD53_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRC61202.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVLKADYSKSGRNASVVKMKMKNL
                     LTGSPTETVYRADDKFDVVMLDRKEVNYSYFADPLYVFMDAEYNQYEIEGECMADALS
                     FLEEAMPCEVVFYEGKAISVELPNSLVREV"
BASE COUNT          930 a         1251 c         1266 g          949 t
ORIGIN      
        1 cgaaccgacc tacacggatt tgcaatccgg tgcataaccg ctttgctatc gcgccgcgcc
       61 tacctcacga taggtcatgc ttttgacgat gaaaccaagc ttgttcttgt tgaaatctgg
      121 agcgggagaa gagtctcgaa ctctcgacct ataccttggc aaggtatcgc tctaccaact
      181 gagctactcc cgcgtgaagc gagtgcgcat tataaaggct tggcaagacc tgtcaacgat
      241 taaggccttt cggtgattca gcctcaatgg ggcgtcaagc gcgtctttgc tgccttccgc
      301 agcccggttc aggccttttt gtccatgtcg tcgagatgcg accacgccat tctcatgtaa
      361 tagcccatcg accagagtgt cagaacagca gcaacgtaga tgagccaggt gcccatgcct
      421 tgtacgtcga agtttccgat tggatcgtga taaagcaaca ttgatatgga gacgatttga
      481 gcggttgtct tgaccttgcc aatcgccgat acggctacgc tcttgtgcgc acccatctgt
      541 gccatccatt cacgcagggc ggaaatggtg atctcgcgcc cgatgatgat tgcggccagt
      601 acggcatcca gcctgccaag ctgaacgagc atgatcagcg ctgcggcaac gaccagtttg
      661 tcagcgaccg gatcgagaaa ggcaccgaac gccgatgttt cgttccagcg gcgcgccagg
      721 taaccatcga gccagtcggt tacgcctgcg agaacgaaaa tcagcgttgc ggctagattg
      781 cgacccacct cgacgatcca tgtttcggga acgtagtaga cgccaatcat caggggtatc
      841 aggacgattc gcagccaggt cagaagtatc ggaatgctga agggcatcaa tgcaaagccg
      901 tgtaaattgt ccgggccagt tcccgactga tgccgggaac tgctgtcagt tggtcgatcc
      961 ccgcattgga gataccttgc aagccgccga aatgagaaat caacgccttg cgtcgttttg
     1021 gtccgatacc ggcgatctcc tcaagtcgtg aagtccgtcg ggtcaagctg cgtcgcgctc
     1081 gatgaccaga aatggcaaac cggtgagcct catcgcgaat ttcctgaatc aggttgagcg
     1141 caggatgttc tgccgccaat tgtagcggtt cgcggccgtc cgcgaaaatc agtttttcaa
     1201 gacccggttt gcgtccttcg cctttggcaa cgcccagcat cggcaaatgc gcgagtccaa
     1261 gttcttccag cgcggacaag gcggcattga cctgaccttt cccgccgtcg atcagaatca
     1321 gggcaggcgc cacgctttcc ccgcttgaga ccttttcata acgccggagt accacctgcc
     1381 ggatggcggc gtagtcatcg ccgggctgga tgccctgaat attgaagcgt cgataatccg
     1441 atttgcgcat gccatcaccc tgatagacca cgcaggaggc gacagtcgct tcgccctgtg
     1501 tgtggctgat atcgaaacac tcgatacgag cctgtttgtt cgcttcgcta tcctcgggct
     1561 ccactccgag acactcgaac agggcgtcaa gacgcgatgc ctgtcgcgaa agaacgttgc
     1621 gacgagcgtg aatggccaat ctggcgttct gctcggccat ctccacccag actttgtgca
     1681 gggttagcct gggttccagt acgggaaccg ggtggccggc cagctcagcc agtgcattga
     1741 ccgtttcact gtcaggcaag gcgcgattga gatagatgcg catcggaatc gggtgtatca
     1801 ggtaatgctg actgaggaaa gcggaaagca cttcttcggg cgaactgtcg ccaacattgc
     1861 tggggaactg cgtcttgtca cccagatgcc ggccaccgcg gaccatggcc agattcacgc
     1921 aaagcaaacc ggcttcctcg acgacaacga cgatatcgac atcctcgcct ttgctgcttt
     1981 cgacgtactg tttgtcttgt atttgacgca aggactggat ctggtcgcga taaaccgccg
     2041 cacgctcgaa agcaagttcg gtcgccgcct gatgcatcgc ttcgccaagt tgcccgatga
     2101 cttcctgttg ccgcccttgc aggaacagcg acgcccgctg aacatcctgc gcataaattt
     2161 caggagcgac gaaatcaacg cagggcgccg aacaacgctt gatctggtag agcaggcagg
     2221 gccgcgaacg gttgctgaat acagaatcct cgcaggtgcg caggcggaac atcctttgca
     2281 ggaggttgag gctttcacgc accgcagtcg ttgacggaaa aggcccgaag tagtcggctt
     2341 tccggtccgt attgccgcga taaaagccca ggcggggata gcggtcgcgg gtgatgacga
     2401 tgtagggata cgacttgtcg tcccggaaaa gaatgttgta gcgtggcgcg aggcttttga
     2461 tcagattgtt ttcgagcagc aaggcctcgg cttcggaccg ggtaacggtc gtctccacgc
     2521 tggcgatctg cgccaccatc aaggcaatgc gcggactggg atggttctcg cgaaagtagg
     2581 acgcgacgcg cttcttcagg ttcttcgcct tgcctacata gagtactctg ccgtcgacat
     2641 cgagcatgcg atacacaccg ggaaactcgg tcagcgtctc cagtagtgca ttggcgtcga
     2701 aagtttcgtc catgggcaag agcagttagg atggcgcctt tgatgttatc acgcgcgttc
     2761 tcctgtccgc gcccggcccc accatgaccc agcctgccaa acctctcgag tgggatatct
     2821 tctgcaacgt catcgacaat tttggcgata ttggcgtttg ctggcgcctt tcgcgccagc
     2881 tcactgtcga gcacgactgc cgggttcgcc tgtgggtcaa cgaactggag gctttccagc
     2941 ggatctgccc ggaaattgat ccgcaaatac ccatccagca cgtgagtggg gttgaggtac
     3001 gccgctgggc aagtacgttc ccggaaagtg ttcccggcga cgtggtcatt gaggcctttg
     3061 gctgccatct gcccgagccc ttcgtcgatg caatggccgg atgcagggcg ccaccggtat
     3121 ggatcaatct cgaatatctg agtgccgaag cctgggtatc gggctgccac accctgccct
     3181 cgccgcatcc gaacctgccg ctcacgaagt atttcttctt ccccggttac ggtgagggca
     3241 cgggcggact gctgcgcgaa cacgatctgg aagaacgcag gcagggcttc tgcacatcgg
     3301 tggaacagca aaaggacttc tggttttcgc tgggccttcc tccgccaccg gccgatgccc
     3361 tggtcgtttc gcttttcgcc tacgaaaatt cggcaatcgg ggatctgctc acggtctggg
     3421 ctcgaggcga ggagccggtc tgttgtctgg cacccgaatc gcgaaccctg gcggcgctgg
     3481 aggaattcgc cggtcaatcg atgcgggcgg gcgataccat ccgccgtggc aagctggaat
     3541 tgcgcgtgct gccttttgtc actcagcagg attacgatcg gctgctgtgg gcgagcgaca
     3601 tcagttttgt gcgcggcgag gactctttcg tgcgcgccca gtgggcggcg aagccgatgg
     3661 tatggcacat ctacccgcaa gacgagggcg cgcatttgag caaactcggc gcctttctcg
     3721 atctgtacac tgcaggcgcc tcacaagcga ccgctgaagc ggttcgcgtg cttcacaacg
     3781 cgtggaatgg tgacctgagc ggcgagagac acgcgcccag gatcaccccg gagttgtggc
     3841 agcagtggat ggatgcgctt cccgaccttc gcaatttggc agaaaactgg gccaatcatc
     3901 tgataaatca ggaagatttg tgcagtagtc tggtgcgttt ctgccgttcc aagttataat
     3961 gcccggctaa tttttcaacg acatttttgg aacgcactat gaaaaccgca caggaacttc
     4021 gcgccggcaa cgtgtttatg gtcggcaacg acccgatggt tgtcctcaag gctgactaca
     4081 gcaaatccgg ccgcaacgcc tcggtcgtca aaatgaaaat gaagaacctg ctgacggggt
     4141 cgccgactga aacggtttac cgcgccgacg acaagttcga cgtcgtgatg cttgaccgca
     4201 aggaagtcaa ttactcctat ttcgccgatc cgctgtacgt ctttatggac gccgagtaca
     4261 accagtacga aatcgaaggc gaatgcatgg ccgacgcgct gagcttcctt gaagaagcca
     4321 tgccgtgcga agtggtcttt tacgaaggca aggccatatc ggtcgaactc ccgaacagcc
     4381 tcgttcgcga agtcat
//
LOCUS       DAORPM010000205         4396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6050_length_4396_cov_7.508408, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000205 DAORPM010000000
VERSION     DAORPM010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6050_length_4396_cov_7.508408"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..723)
                     /locus_tag="PLD53_09560"
     CDS             complement(97..723)
                     /locus_tag="PLD53_09560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC61203.1"
                     /translation="MTSEHTTTYFDARAKTWDDDPMKTARAQAVADGIQSRVPLTNHM
                     TALEYGCGTGLLSFALQPQLKHITLADSSSGMLAVLQEKIDTNGIANMRAVQMDLTTD
                     PLPADRYTLIYSLMTFHHIDDIDRILRDLFSLLVTPGYLCIADLDSEDGSFHGPEFTG
                     HRGFDRIEFERKLIHAGFRSVDFTTVFEITKGEKQTRYPVFLMIARKL"
     gene            935..3214
                     /gene="pbpC"
                     /locus_tag="PLD53_09565"
     CDS             935..3214
                     /gene="pbpC"
                     /locus_tag="PLD53_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRC61204.1"
                     /translation="MISRSSRITCLVTLCLATFCVPASAVPSFSEVRAAYIASDALLL
                     ARDGRPLQSTRIDMTQRRLPWTRIEDVSPAFLRALLASEDRKFYEHAGVDWSAAAVSA
                     WRNLWNTRTRGASTLSMQLIGLLDELDDGTTQRHGRRSVPQKLSQAAGALWLEQRWKK
                     DEILEAYLNLASFRGEMVGVTAMSRGLFGKWPDGLDDRESALAAALLRAPNASARVVA
                     TRACGVLQAMAQMKQGASADCEGLDGLARLALSGGLRDRFDSAGWLGAEQSPGLAPHL
                     AQKLLTQPGQRLTSTLDADLQRFAVATLQRQLRGIARQNAEDGAVLVLDNASGEVLAW
                     VGSSGALSDAPEVDGVTALRQAGSTLKPFLYALAFERRDLTAASLIDDSPLALTTGNG
                     LYVPQNYEPQYRGWVSARMALGGSLNVPAVRTLVRIGPEAFRDRLYAFGFKSLTENGE
                     YYGFSLALGSAEVSLLMLANGYRTLANGGEWSPLRVTRLANHRPCVDGGCSGVFGADS
                     ERSKHREFSSRRVADAAPVFIVADILADRAARVGTFGLESWLATPYWSAAKTGTSKDM
                     RDNWCVGFSSRYTVAVWVGNAAGEAMHDVSGISGAAPIWREVMDRLHRGSSHPPSVAK
                     PPPVGVARQSIRFEPAGRIDEAARQEWFIAGTGMSVVRLAESRALAHIAYPGQGTIIA
                     LDADIPPERQRIALQLSGLPAPGWQWRIDGKPAGMASDNVLWRPSPGHHHLVLADANG
                     RELETVRFEVRALKGKRAK"
     gene            complement(3229..3966)
                     /locus_tag="PLD53_09570"
     CDS             complement(3229..3966)
                     /locus_tag="PLD53_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61205.1"
                     /translation="MDESEREAEKQKCGELLRRHLQAAKALRQRANKSPAAARKRLLL
                     REWQAARLARCYADLLASERYAQSARFILTDLYGPKDFSSRDDEVEKILPLLLNLLPL
                     SALQTITLAVEIDALTEKLDAATVVELDRAGMIDRIDEDNYADAYRRVRRQKDRMRQI
                     ALIRKTGDALERLARKPLVSTVLKLMRRPAHLAGLGDLHAFLENGFDTFRRMGRASEF
                     LDCIEDRERKLVSNLFDGVARPFDLLA"
     gene            4077..>4396
                     /locus_tag="PLD53_09575"
     CDS             4077..>4396
                     /locus_tag="PLD53_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61206.1"
                     /translation="MLATVLRLFILFELTVYSALAWYFLDATVLSAALYAVAGILSLR
                     LWITGLTYAFAWFQHSPWPTLGIWQAIRMFFGECAAFILNFVLVSPFERFWMGTDRLT
                     PTGER"
BASE COUNT          749 a         1248 c         1450 g          949 t
ORIGIN      
        1 actccggctg gccgggaagc tgcgcttgca agcaagctga ttctcatcaa cagctgcgtc
       61 cttacgcagg gtgtggaaag catgttcgat agacggttac agctttctgg cgatcatcag
      121 gaacaccggg tagcgcgtct gtttttcgcc tttcgtgatt tcgaagaccg tcgtgaaatc
      181 gaccgagcga aatccggcgt ggataagctt gcgttcgaat tcaatcctgt caaagccccg
      241 gtggccggtg aattcggggc catggaacga gccgtcctcg ctatccagat cggcgatgca
      301 caggtagcct ggagtgacga gcagggagaa aagatcacgc agtatcctgt cgatgtcatc
      361 gatgtgatga aacgtcatca gtgaatagat cagggtgtaa cggtcggcgg gcagggggtc
      421 ggtggtcaga tccatttgaa cggcgcgcat gttggcaatg ccattcgtat cgatcttttc
      481 ttgcagcacc gccagcatgc cggaggaact gtccgccagg gtgatgtgct tgagctgcgg
      541 ttgcagggcg aaactgagca agccggttcc gcaaccgtat tcaagcgcgg tcatgtgatt
      601 ggtcagcgga acccgcgact gaatgccatc ggcaacggct tgcgcccgcg ccgtcttcat
      661 gggatcgtcg tcccaggtct tggccctggc atcgaagtag gtcgtcgtat gttcgctggt
      721 catgcgtgtt cctgaatggg tggttaccga ggcaagcagg gtgagctggt gacattctac
      781 tttgaccgcc cagttgatat gagtcgtttg tgcccggtgt gcaagaatgg atgactgtgt
      841 ggctctgctt gtcttttgat cgatggggtg ttgggatgga gccttctgaa atcagaccga
      901 ttttcatagg ccgactacgt tgtcggccgt gatcatgatc tctcgttcct ctcgaatcac
      961 gtgcctggtc acgctttgtc tggcgacgtt ttgcgtgccg gcgtcggcgg taccttcgtt
     1021 ttccgaggtg cgcgcagcct acatcgcgtc cgatgccctg ctgctcgccc gcgatggccg
     1081 gccattgcag agtacgcgca tcgacatgac gcagcgccgt ttgccgtgga cgcggatcga
     1141 agacgtttcc cccgctttcc tgcgggctct gctggcttcg gaagatcgca agttctacga
     1201 acacgccggc gtcgattgga gcgctgccgc agtcagcgct tggcgcaatt tgtggaatac
     1261 ccggacacgg ggcgccagta cgctttcgat gcagctcatc ggcctgctcg acgagcttga
     1321 cgatgggact actcagcgtc atggccggcg cagcgtcccg caaaagctgt cgcaggcggc
     1381 cggcgcattg tggctcgaac agcgctggaa gaaggacgag atccttgaag cctatctcaa
     1441 tctggcgagc tttcggggtg agatggtcgg tgttacggcg atgagtcgcg ggctgttcgg
     1501 caagtggccg gatggtctgg atgatcgtga atcggccttg gcagccgcgt tactgcgcgc
     1561 acccaatgct tctgcacgcg ttgtggcgac gcgggcctgc ggcgtgttgc aggcaatggc
     1621 gcagatgaag cagggtgcca gcgcagattg cgaagggctg gatggcttgg cgcgattggc
     1681 gctttccggc ggcttgcgtg accgatttga cagtgccggt tggcttgggg cggaacagtc
     1741 tccgggattg gcgccgcatc tggcgcagaa gctgctcacg cagccggggc aacggctgac
     1801 gagcacgctg gacgccgatc tccagcgatt tgcggtggcc acgctgcaac ggcagttgcg
     1861 gggaatcgcc cggcagaatg cggaagacgg cgcggtgctg gtactcgata acgcctcggg
     1921 cgaggtgctg gcctgggtcg gttctagcgg ggccctgtcc gatgcgccgg aagtggatgg
     1981 cgtgaccgcg ctgcggcagg cgggatcgac gctcaagcct tttctgtatg cgctggcatt
     2041 cgaacggcgc gacctgacgg ccgcctcgct gattgacgat tcgccgctcg cattgacgac
     2101 cggcaacggc ctctacgtgc cgcagaatta cgagccgcag tatcgcggct gggtcagcgc
     2161 gcgcatggcg ctgggcggct cgctcaacgt gccggcggtg cgcacgctgg tgcgtatcgg
     2221 gcccgaggct ttccgcgatc gcctgtacgc cttcggcttc aagagcctga ccgagaacgg
     2281 cgagtactac ggtttcagtc tggcactggg ttcggccgaa gtttccctgc tgatgctggc
     2341 caatgggtac cgcaccctgg ccaacggtgg cgagtggtcg cccttgcgcg tgactcggtt
     2401 ggcaaaccat cggccctgtg tcgacggagg ttgcagcggc gtctttggcg ccgactcaga
     2461 acgctcgaaa caccgggagt tctcttcgcg gcgggtcgcg gatgccgcgc cggttttcat
     2521 cgtcgccgat attcttgccg accgcgccgc gcgggttggc accttcgggc ttgaatcgtg
     2581 gctcgccaca ccgtactgga gcgccgcgaa aaccggcacc agcaaggaca tgcgcgacaa
     2641 ctggtgtgtc ggattctcgt cccgctatac ggttgcggtg tgggtcggca acgccgctgg
     2701 cgaggcgatg cacgatgtgt cgggaatttc aggcgcggcg ccgatttggc gcgaggtgat
     2761 ggaccgcctg catcgtggca gcagtcatcc gccaagcgtg gccaaaccgc cgcccgtcgg
     2821 tgtggcgcgc caatcgatcc gctttgagcc ggccggcagg atcgatgaag ccgcccggca
     2881 ggagtggttt atcgccggca ccggcatgag cgtcgttcgc ttggccgaga gcagggcgtt
     2941 ggcgcacatc gcctatccgg ggcaggggac gatcatcgcg ctcgacgcgg atattccgcc
     3001 cgaacgccag cgcatcgcat tgcagctttc cggcctcccg gctcccggct ggcagtggcg
     3061 aatcgacggc aagcctgcgg gaatggcgtc agacaacgtg ctctggcggc cgtctccagg
     3121 acatcatcat ctggttctgg ccgatgcaaa cggtcgcgaa ctggaaacgg tccgttttga
     3181 ggttcgggcg ctcaagggaa aacgggcgaa atagccaaga aatctttctc aggcgagtag
     3241 atcaaaaggc cgtgcaacac catcgaacag attgctgacc agcttccgtt ccctgtcttc
     3301 aatgcagtcg agaaactcgc tggcgcggcc catgcgtcgg aaggtatcga agccgttttc
     3361 gagaaaggcg tgcagatcgc cgagcccggc gaggtgagcc gggcggcgca tcagtttcaa
     3421 cacggtcgag accaggggtt tcctggcgag cctttccagc gcatcgcccg tcttgcggat
     3481 gagagcgatc tgacgcatgc gatccttttg gcggcggacg cgccggtagg cgtctgcgta
     3541 gttgtcttca tcaatgcggt cgatcattcc ggcgcgatcc agttcgacga ctgtcgccgc
     3601 gtcgagtttc tcggtcagcg cgtcgatttc tacggccagc gtaatggttt gcagggcaga
     3661 gagcggaagc agattgagca gcagaggcag aattttttcg acttcgtcgt cgcggctgct
     3721 gaagtccttc gggccgtaga ggtcggtgag tatgaagcgt gcggactgag catagcgttc
     3781 gctggccagc agatcggcat agcatcgggc gaggcgggcg gcttgccact cacgcagaag
     3841 caagcgcttg cgcgcggcgg cgggcgactt gttggcgcgt tgccggagcg ccttggccgc
     3901 ctgcaggtgg cggcgcagta gttcgccgca cttctgtttt tctgcttcgc gttcggattc
     3961 atccatggtg attgattgtg tccggctgac catgcaagcg tgtcattggt atgatgcatt
     4021 ctacagcgac gctcgtttca cactattcaa ggatcattca acgatcacgg tccaatatgc
     4081 tcgcaaccgt actccgcctg tttatcctgt ttgagctgac cgtgtattcg gcattggcct
     4141 ggtatttcct tgatgccacg gtgctatccg cagctctgta cgcggtggcg ggtatcctga
     4201 gcctgcgctt gtggataacc ggcctgacct atgcattcgc ctggttccag cattcgcctt
     4261 ggccaacgct cgggatctgg caggccattc gcatgttctt cggtgagtgc gccgccttta
     4321 tcctcaactt cgtgcttgtt tcaccctttg aacgcttctg gatgggcacc gatcgcctga
     4381 cgccgacggg cgaacg
//
LOCUS       DAORPM010000206         4372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6107_length_4372_cov_8.360204, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000206 DAORPM010000000
VERSION     DAORPM010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6107_length_4372_cov_8.360204"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLD53_09580"
     CDS             <1..393
                     /locus_tag="PLD53_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61207.1"
                     /translation="AFAFWAFSLSYSTSYFEYSLASRLPPFLAALAGAIAIPVSVWYQ
                     LQRVGRSRHSMVRAWVMHVCTSIAAVAPLAATAAVLSRIRGPMHLSGDDAMGVGLNFI
                     FLTATAILSVLFLSVVLVLKHRRASRVA"
     gene            556..1041
                     /locus_tag="PLD53_09585"
     CDS             556..1041
                     /locus_tag="PLD53_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRC61208.1"
                     /translation="MKYCPLCRSDLENRPVDGVVRLACSSVECQFVSWNNPIPVVAGL
                     VRHEGQYLLARNATWPQGMFSMITGFLEQGESPESAILRETEEELGLKGSECKFIGHY
                     AHPRFNQLIIAFAVEASGDVKLNDEIAEVRMVSPEELATYDFGRLGLIRTIVTHWLER
                     A"
     assembly_gap    1426..1525
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1526..2681
                     /locus_tag="PLD53_09590"
     CDS             <1526..2681
                     /locus_tag="PLD53_09590"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HRC61209.1"
                     /translation="DVTAPNADTLYSSTWLDVGQEPYVLSLPEADNRYYLFPMLDGWT
                     TVFQVPGKRTTGTGPQKYVITGPNWKGELPVGIPEYKSPTSIVWVLGRIYSTGTPEDY
                     AKVHKLQDKISLVPLSSFRRPYLPPVGKIDPAIDMKTPVREQVNALDTAAFFNLLATL
                     MKDNPAAPADAPIVVKMEQLGIKPGTPFAFAELDPAVQAELQDVPKVAFEKIMAHFKR
                     AGKNVNGWVFTTNTGTYGTDYLQRALITAIGLGANRPQDAVYPTSQADAKGRPYSGKY
                     KYVMHFPKGKLPPVDGFWSLTMYNADYFFYGNPLNRYTLSARNDLKANADGSVDLYLQ
                     HENPGPEKESNWLPAPEGKFILMLRLYWPKETPPSIIDGSWAIPPVVKVK"
     gene            2744..3601
                     /locus_tag="PLD53_09595"
     CDS             2744..3601
                     /locus_tag="PLD53_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC61210.1"
                     /translation="MKFRSLVAAGFAVSCLFAVPAVANEPTLTTIKQSSTIKLGYREN
                     SPPFSFVGDDKKPRGYSVDLCLLVATDIAKQLSIPNLEVKWVPVSAQSRFDALKKGEI
                     DIECGNTTQTLTRRADFDFSLMTFVDGAGLLFRAGENPKSMEELKGQRIVVVAGTTTE
                     KTMEAFVADTKLGVKLIKAKDHDTAMASLQDKTATAYAADRTVLITTALLRGEGKDFV
                     VADNQFSYEPYGLMMRRDSDMRLAVDRTLANLYRSGDIGPVLQNWFGPLGKPSEVLKV
                     MIYLNGLPD"
     gene            3718..>4372
                     /locus_tag="PLD53_09600"
     CDS             3718..>4372
                     /locus_tag="PLD53_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRC61211.1"
                     /translation="MSAQDSTLFSSLDIGQISLPGRLFKTATAETRGTEDGFATDAVT
                     EFYVPIARGGTPLIITGNIYVSRDGKSAPLQLGADNDDKILALSKVVDAVHEQGAKFF
                     AQLNHCGRQVIPPFAQSPEAFSPSNVKDLLTGTRPKALTVPQIQRLVGQFADAAARCQ
                     RAGFDGIQLHSANGYLLSQFLTPYTNRRTDEYGGTMENRTRFIREIIRAIHTRLGPDF
                     "
BASE COUNT          895 a         1226 c         1178 g          973 t
ORIGIN      
        1 gcttttgcat tctgggcctt cagcctgagc tacagcacgt cctatttcga atatagcctt
       61 gcgtcgcgct tgccgccgtt cctggctgcg ctcgcgggtg ccatcgcaat tcctgttagt
      121 gtctggtacc aattgcaacg tgttggccgc agccgtcatt cgatggtgcg cgcctgggtt
      181 atgcatgtct gtacgtcgat cgccgccgtt gcgccactcg ccgccaccgc cgcagttctc
      241 tccagaatcc gcggaccgat gcatctctcg ggcgacgatg cgatgggcgt cggtctgaat
      301 ttcattttct tgactgcgac cgccatcctt tctgtattgt tcctgagcgt tgtgcttgtt
      361 ttgaagcacc ggagggcgtc tcgtgttgct tgattcatca aatttgcact ccgccaaaag
      421 agaacttgtg cgattggcag taatcatcag aagtaactga cagtctgtcg gtttgttgtg
      481 ggccatgccc ggttcgcgga aagtgaaaca ttctggtgtc ttgatattcc atttcgttca
      541 cggcacgatg cgtcgatgaa atactgtccg ctttgccgaa gtgaccttga aaaccgtcct
      601 gttgacggcg tcgtgcgcct ggcgtgttcg tcggttgagt gccagttcgt gagctggaac
      661 aatcccatac ccgtggttgc cggccttgtg cggcacgaag gccagtatct tctagcgaga
      721 aatgccacct ggccgcaagg catgttctcc atgatcaccg ggttccttga gcagggcgag
      781 tcacccgaga gcgccattct cagggaaacg gaagaggaac tcggcctcaa gggcagcgag
      841 tgcaagttca tcggacatta cgctcacccc aggttcaatc agctcatcat tgctttcgct
      901 gtcgaagcca gtggcgatgt gaagttgaac gatgagatcg cagaggtgcg aatggtttcg
      961 cctgaggagc ttgccaccta cgacttcggg cgacttgggc taatccgaac aattgtcacg
     1021 cattggctgg aacgcgcctg acgcggtggt cgcgtcaacg cgctttcggt gcttgattgt
     1081 tcatggctag ccattcattc ggcgatccgc gcatttcccc gtacccgggc agcgtagcct
     1141 ggtaaaggtt gtcgtagtag gtgcctggag tagaatcatc acaaagtcag caaggcagga
     1201 tcatgctgat ctgcgcttct ctttatcccg attgggcctt atgaaacacc gcgtattcat
     1261 ctttttcgct gtactgatac ttgtcgcttg ctcgcgcatg gatagaagta tcagtgcaaa
     1321 gatcacagaa ttgttcgacg ccagtaattc ggcgccaatc gaccttgcgc aggtcggacc
     1381 accttcatgg gagcgtgtct gcgtcttggg cccatacgtc accaannnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnggatg tcaccgcgcc caatgccgac acgctctaca
     1561 gctccacctg gctcgatgtg ggccaagagc cctacgttct gagtcttccg gaagccgata
     1621 accgctacta cctcttcccg atgctcgacg gctggaccac ggtctttcag gtgccaggga
     1681 agcgcaccac cggtaccgga ccgcagaagt acgtcatcac cggcccaaat tggaaaggcg
     1741 aactccctgt cggtatcccg gaatacaagt caccgaccag catcgtctgg gtcctcggcc
     1801 gtatctacag caccggcacc ccggaggatt acgccaaggt ccacaagctg caggacaaga
     1861 tctcgctcgt gcccctgagc tcgtttcgca gaccatacct gcctccggtt ggcaagatag
     1921 atcccgccat cgacatgaag actccggtgc gcgagcaggt gaatgccctg gatactgccg
     1981 ccttcttcaa tctgctggcg acattgatga aggacaatcc ggctgcgcca gcggatgcgc
     2041 ccatcgtcgt aaaaatggag caactcggca tcaagcctgg tacgccgttc gcattcgccg
     2101 aactcgatcc tgccgtgcag gccgagctgc aagacgtgcc caaagttgcg ttcgaaaaaa
     2161 tcatggcgca cttcaagcgt gccggtaaga acgtcaatgg ctgggtgttc actaccaaca
     2221 ccggaactta tggcaccgac tacctccagc gggcactgat aaccgccatt ggcctgggcg
     2281 ccaaccgtcc tcaggatgcg gtttatccca cttcgcaagc ggatgccaag ggccggccct
     2341 acagtggtaa gtacaaatac gtcatgcact tcccgaaggg caaattgccg cccgttgacg
     2401 gtttctggtc gctgaccatg tacaacgccg actacttctt ctacggtaac ccgctcaatc
     2461 gctacacgct cagcgcgcga aacgatctga aggccaacgc ggacggctcg gttgacctct
     2521 atcttcagca cgagaacccc ggtccggaaa aagagtccaa ctggctcccg gcacctgagg
     2581 gcaagtttat tctgatgctc cgcctctatt ggcccaaaga aactccgccc tccatcatcg
     2641 acggtagctg ggcgattccg ccggtggtga aggtaaaata ggtttctggc attctgttgt
     2701 ttttttcaac gggccgaatt ggccccttaa cgaaggattt aatatgaaat tccgttctct
     2761 cgtcgctgct ggttttgcag tttcctgcct ctttgcagta cctgcggtgg ccaatgaacc
     2821 caccttgacc acgatcaagc aatcttcaac gattaagctc ggctaccgtg agaactcccc
     2881 gccgttttcg tttgttggtg atgacaagaa gccgcgcggc tacagtgttg atctgtgctt
     2941 gctggtagcg actgacatcg ccaagcagtt aagtatcccg aatctggaag tgaagtgggt
     3001 tccggtgagc gcacagagcc gcttcgacgc tttgaagaaa ggcgaaatcg acattgagtg
     3061 tggcaacaca actcagacac tgacgcgtcg cgccgacttt gacttcagcc tgatgacctt
     3121 cgttgatggc gccggtttgc tgtttcgcgc cggcgaaaat ccgaaatcga tggaagaact
     3181 caaggggcag cgcatagtcg tggtggccgg cacgacgacc gaaaagacaa tggaggcgtt
     3241 tgttgcggac acaaagctcg gcgtaaagct gatcaaggcc aaggaccacg acacggcgat
     3301 ggcttcgctg caggacaaga cagcaacagc ctatgctgcg gatcgtaccg tattgatcac
     3361 cacggctctg cttcgtgggg aaggcaagga ttttgttgtc gctgacaatc aattttccta
     3421 cgagccctat ggtttgatga tgcgtcgcga ttcagatatg cgacttgccg ttgatcgcac
     3481 gctggcaaat ttataccgta gcggcgatat cggaccagtc ctccagaact ggttcggtcc
     3541 gctcggcaag ccatctgaag tgctcaaggt gatgatttac ctgaacggtc tgccggatta
     3601 actcggaagc gcgcaagcag cttgaattgg gccggggatg atgggtcgga tgcattgctc
     3661 cgatggcatc tggattctgc tctgaccctt ttcggctgat ctcgtgagag gcattccatg
     3721 tcagcgcaag acagcactct cttcagctcg ctcgacatcg ggcagatttc cctgcctggc
     3781 cgtttgttca agacagccac cgcggaaacg agaggtacag aagacggctt cgcgacggat
     3841 gcggtgactg aattctatgt gccgattgcg cgcggcggca caccgctgat cattaccggc
     3901 aacatctacg tcagccgaga tggcaaatcg gcaccgctgc aattgggtgc cgacaacgac
     3961 gacaagatat tggctctgtc caaggtcgtc gacgccgttc atgaacaggg tgcgaagttc
     4021 tttgcccagc tcaatcattg cgggcggcag gtgattccgc catttgccca gtcgcccgaa
     4081 gcgttttctc cctccaacgt gaaggatctg ctgaccggca ccagaccgaa agctctgacg
     4141 gtgccacaga tccagcgctt ggtcgggcag ttcgccgatg ccgccgcgcg ttgtcagcgc
     4201 gctggatttg acggcataca actgcattcg gcgaacggct acctgctgag ccagttcctc
     4261 acgccctata ccaatcggcg aaccgacgaa tatggcggca cgatggagaa tcgcacgcgc
     4321 ttcattcgcg agatcattcg ggcgatccac acgcgcctcg gcccggactt tc
//
LOCUS       DAORPM010000207         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6120_length_4367_cov_9.409787, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000207 DAORPM010000000
VERSION     DAORPM010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_4367_cov_9.409787"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLD53_09605"
     CDS             complement(<1..365)
                     /locus_tag="PLD53_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61212.1"
                     /translation="MNSAVPERQGLYDPAHEHDACGVGFVAHIKGLKSHSIIQQGLLI
                     LKNLNHRGAVGADPLMGDGAGILIQIPDEFFRKEMASRGVILPRAGDYGVGMVFLPKE
                     SASRLACQEAIERAVRTEG"
     gene            complement(747..1514)
                     /locus_tag="PLD53_09610"
     CDS             complement(747..1514)
                     /locus_tag="PLD53_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HRC61213.1"
                     /translation="MKRWWFNPDYANGEAERLFGTLDTTFATKGEQVAKSPVSRVLRV
                     TAGDKHYYVKRYLGNGKNAVRRWFGLRGLVAPQRVVKEWKNLLLFRKWGIPTATLVGY
                     GLERKYGSFVRGALITEEIPNTTDLERLATASDPCLRDRRWMAQVLRQVANITRTLHA
                     AGFAHNDLKWRNLLVDGGGSPTVYLIDCPSGGMWWGVFLKYRIIKDLACLDKVAKYQL
                     SRSQRLRFYLDYTGQRRLGVEDKQRIRKILAFFEGRE"
     gene            complement(1617..2522)
                     /locus_tag="PLD53_09615"
     CDS             complement(1617..2522)
                     /locus_tag="PLD53_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC61214.1"
                     /translation="MRTSRSEFVSLRARRHHVRVWGEAGAPTFFFLHGWGDVGASFQF
                     VVDALRRNWRIIAPDWRGFGQSQWNEGAYVFADYIADLDALLTHYSPEQPVQIAGHSL
                     GGIVASLYAGIRPERVSRFANLEGFGLWVSAPGETPDRFGKWLQQIRDNNPAFRPYAN
                     REDYAARLCADNPRLTPARASFLADHSLREVDTDPGASAGFVFAADPRHRWISPVLYP
                     LDEAKACWRKVSAPTLWVAGRDSKIMKQFADRPDDYRERMACFAQVEERLIDNCGHNL
                     HHDQPVIIANLLDEFFLRDLHINAD"
     gene            complement(2526..3752)
                     /locus_tag="PLD53_09620"
     CDS             complement(2526..3752)
                     /locus_tag="PLD53_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC61215.1"
                     /translation="MFRSLNTPALWAVTLSAAGILMVTMGARQSLGLFLAPLNTTTGL
                     GIASISLAMAVGQFTWGAVQPLAGAVADRFGPRRVLQGGLLLLALGSAITPFMSSTWG
                     LVFSLGLISAMGSGAGSFSVLIGAASTRLPLEARGTASGIINAGGSFGQFVFAPVLQK
                     LIQAMGWMGSMWALALMTLAALPLIGTVSRPVDPVHQPAATTEIGLRRSVQEALADRS
                     YLLLHAGFFTCGFHIAFLVTHLPGEVDLCGLPPSVASWSLAIIGLANIFGSLFAGACV
                     SRYRSKYVLFWMYASRAILIALFLAAPRTDLTFYLFAAGLGFTWLATVPPTAAIVGKL
                     FGVRYLGTLFGLTLLSHQIGGFLGAWLGGLSITQFGDYQWMWYADMVLASLAALVNLP
                     IREAAVLQPDAASASA"
     gene            complement(3862..>4367)
                     /locus_tag="PLD53_09625"
     CDS             complement(3862..>4367)
                     /locus_tag="PLD53_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HRC61216.1"
                     /translation="RDDDPQLNVRAVATSRQPLKVVVDSALELPLESRVLNDGGVLVA
                     TASEECEKSAALRARGAEVVCFPNAIGKVDLPALLDELGRRGINELHVEGGLRLNGAL
                     LAAGLVDELLIYLAPCLIGNAALGMFDLPALATLEDRHRLAIRDLRMVGPDVRILARF
                     AANERPA"
BASE COUNT          921 a         1332 c         1330 g          784 t
ORIGIN      
        1 tgcccttctg tgcgcactgc acgctcgatg gcctcctggc atgccaggcg cgaagcggat
       61 tccttgggca ggaataccat cccgacgccg taatcgccgg cgcgcggcag aatcacgccc
      121 cgactggcca tttccttgcg gaaaaattcg tccggaatct ggatcagaat gccagcgccg
      181 tcacccatca aaggatccgc gccgacagcg ccccgatgat tcagattctt cagaatcagc
      241 aaaccctgct ggatgatcga gtggctcttc aggcccttga tatgcgctac aaaaccgaca
      301 ccgcaggcgt cgtgctcgtg ggccgggtca tataaccctt gccgttcagg tacagccgaa
      361 ttcatcacgc ttaaatcctc gaaatacggt tggtaaaaca tcaagaacgc tggtcaaaag
      421 ttgctcatga atactacgaa cggccgggga gtatactccg aaatatctac cgttcaaatc
      481 cttgcgccgc accaatagag tgcaaatagg agtgcgctca aggcgtctca gccgcacgga
      541 accactgcca ctcgccggta ttcctgacgg cgagttgtca ggcctatcgc cgtgcatgca
      601 agtgccgtgc ctgcccggcg cgtcatccat gtcatctgct ctggcatgca ggttgccaaa
      661 gggcactcgg gatgacctcc tgcgccctgt aatccggaat cagcaatgtg accagcaatt
      721 gcgctggttt gttcactgga aacggatcac tcgcgtccct caaaaaacgc aagaattttg
      781 cgtattcgct gcttgtcttc cacgcccaat cgtctctgcc cggtgtaatc aaggtagaag
      841 cgcaagcgct ggctgcggga aagctggtac ttggccactt tgtcgaggca agccagatcc
      901 ttgatgatgc ggtatttcag aaacacgccc caccacatac cgccgctagg gcaatcgatc
      961 aggtacaccg ttgggctgcc accgccatcc accagcaggt tgcgccattt cagatcgttg
     1021 tgggcaaaac cagccgcgtg cagcgtgcgg gtaatgttgg caacctgccg caagacctgg
     1081 gccatccagc gccggtcacg caggcatggg tcgctggcgg tcgccaacct ctccagatcg
     1141 gtcgtgttcg ggatttcctc ggtgatcagt gcaccgcgca cgaaactgcc gtacttccgc
     1201 tccagtccat accccaccaa tgtggctgtc gggatacccc atttccggaa cagcagcagg
     1261 tttttccact ccttgacgac gcgctggggg gcaaccagtc cacgcaggcc gaaccagcgc
     1321 cgtacggcat tcttgccgtt gccaagatag cgtttgacgt aatagtgctt gtcgcctgcc
     1381 gtcacccgta gcacacggct aacaggtgac ttcgcaactt gctccccttt cgtggcgaag
     1441 gttgtatcca gtgtgccaaa caagcgctcg gcctctccgt tggcatagtc ggggttaaac
     1501 caccagcgct tcattcggcg cgcctgggac tcgcggaaat cactgctacc cgatgattgc
     1561 agaggcagcg ggttgtcagg cttgaagcca gacctgcaag gtgtgcggaa ttcatgtcag
     1621 tcagcgttga tgtgcaggtc gcgtagaaaa aattcgtcca gcagattcgc gatgatcacc
     1681 ggttggtcat ggtgaagatt gtgtccgcag ttgtcgatca ggcgctcctc cacttgggca
     1741 aagcaggcca tgcgttcccg gtaatcatcc ggtcggtcgg cgaactgttt catgatcttc
     1801 gagtcgcgac cggcgaccca gagcgttggc gcgctcactt tccgccagca cgccttggct
     1861 tcgtccagcg gatagagcac cgggctgatc cagcgatggc gcgggtctgc ggcgaaaaca
     1921 aaaccggcgc tagcgccggg gtccgtgtcg acctcacgca acgaatgatc ggcaaggaat
     1981 gacgcacgtg ctggcgtcag acgcggattg tccgcgcaca gtctggcggc atagtcttcg
     2041 cgattggcgt agggacggaa cgccgggttg ttgtcgcgga tctgctgtag ccattttccg
     2101 aaccggtcgg gggtttcgcc gggagccgag acccacagtc cgaagccttc aagattggcg
     2161 aagcgggata cccgctccgg gcgaatgccg gcgtagaggc tggcgactat gccgcccagg
     2221 ctgtgcccgg caatctgcac cggttgctcc ggggaataat gggtcagcag ggcgtccaag
     2281 tccgcaatgt agtcggcgaa aacgtaggcg ccttcgttcc attgcgactg gccgaatcca
     2341 cgccagtcgg gcgcgatgat ccgccaattg cgccgtagcg cgtcgacgac aaactgaaac
     2401 gaggcgccga catcgcccca gccgtgcagg aagaagaacg ttggcgcacc ggcttcgccc
     2461 cagactcgga cgtgatggcg ccgcgcgcgc aatgaaacga attcggagcg ggacgtgcgc
     2521 atggttcagg ccgaggccga cgccgcatcg ggttgcagaa cggccgcttc gcgaatcggc
     2581 aggttgacca gcgccgcgag gctcgccagt accatgtcgg cgtaccacat ccattgatag
     2641 tcgccgaatt gcgtgatcga caggccgccc agccaggcac cgagaaagcc gccgatctga
     2701 tgcgagagca gggtcagccc gaacagcgtt ccgagatagc gaaccccgaa cagcttgccg
     2761 acgatggcgg cggtcggcgg tacggtggcc agccaggtga aaccgaggcc cgcggcgaag
     2821 agataaaacg tcagatcggt tctgggtgca gccagaaaca gcgcgatcag tatcgcgcgc
     2881 gaagcgtaca tccagaagag cacatatttg ctgcgataac gggaaacgca ggcaccggcg
     2941 aaaaggctgc cgaagatgtt ggccaggccg atgatggcca gcgaccaact ggccaccgag
     3001 ggcggcaggc cgcacagatc gacctcgccc ggcagatggg tgacgagaaa ggcaatgtgg
     3061 aaaccgcagg taaaaaatcc ggcgtgcaac agcagatagc tccggtcagc cagcgcttcc
     3121 tgaacgctgc gccgcagccc gatctcggtt gtggccgccg gctgatgcac cgggtcgacc
     3181 ggacgggaca ccgtcccgat cagtggcagc gcggcgagag tcatcaaagc cagagcccac
     3241 atggacccca tccagcccat ggcctgaatc agtttttgca ggacgggagc aaagacgaat
     3301 tgcccgaagg agccacccgc gttgatgatg ccggaggccg tgccgcgtgc ttccagcggc
     3361 aggcgggtgg aagcggcgcc gatcagcacc gagaaactgc cagcgcctga acccatggcc
     3421 gaaatcaagc ccagtgagaa gaccagtccc caggtgctgc tcatgaaagg cgtgatggca
     3481 ctaccgagcg ccagtagcag caggccgccc tgcagcaccc gacgtggccc gaaacggtcg
     3541 gccacggcgc cggccagcgg ctgcacggcg ccccacgtga attgcccgac ggccatggcc
     3601 aggctgatcg aagcaatgcc gaggccggtg gtcgtattga gtggtgccag aaagaggccc
     3661 aacgactgac gcgcgcccat cgttaccatc aggatgccgg cggccgagag tgtgacagcc
     3721 catagagccg gagtattgag cgagcgaaac atgagtcggt tttccagggc cttcgacagg
     3781 cggtttgagt gtcagtcgcg agcggagttc aaaggatgcc agcaaagcgc gttgattgct
     3841 ggtgattcgc agacactgtc gtcaagccgg ccgttcattc gctgcgaatc gagccagtat
     3901 tcgcacgtcg gggccgacca tgcgcagatc gcgaatggcc agtctgtgac ggtcttccag
     3961 tgttgcaagt gcgggcaggt cgaacatgcc gagcgccgca ttaccgatca ggcagggcgc
     4021 aagatagatc agcaattcat cgaccagtcc agccgccagc aaggccccgt tgagtcttaa
     4081 gcccccctcg acatgcagct cgttgattcc gcgtcgcccg agttcatcca gcaatgccgg
     4141 cagatccacc ttgccgatag catttgggaa gcacacgact tcggcaccac gggcgcgcag
     4201 tgcggcggat ttttcgcatt cttcgctcgc ggttgccacc agtaccccgc cgtcgttcag
     4261 aaccctggat tcgagcggca attcgagagc gctatcgacc accactttca gcggctggcg
     4321 cgaggtggcc acggctcgga cgttaagctg cgggtcgtcg tcgcgta
//
LOCUS       DAORPM010000208         4343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6176_length_4343_cov_9.531017, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000208 DAORPM010000000
VERSION     DAORPM010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6176_length_4343_cov_9.531017"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..2095)
                     /gene="glmS"
                     /locus_tag="PLD53_09630"
     CDS             complement(266..2095)
                     /gene="glmS"
                     /locus_tag="PLD53_09630"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRC61217.1"
                     /translation="MCGIVAAVADRNVVSVLLEGLRKLEYRGYDSAGLALINGSGLQR
                     LRAVGRVAELTTQADQSSAYGNTGIAHTRWATHGAPSERNAHPHISEGLAVVHNGIIE
                     NHEVLRARLKSEGYEFTSDTDTEVVGHLIASELKKCSDFFAATRAAIAQLQGAYALAV
                     LRESEPEHVVVAREGSPLLLGLADDGSYAASDASALLQVTRRIVYLENGDCAELSRDS
                     YRITRVDGSPVERVETESHLSADAVELGQYRHYMQKEIFEQPVALSNTLEMLGAARSI
                     QPGLFGADTESILKDVRQVLIIACGTSYHAGLVARYWLEAIAGISCNVEVASEYRYRE
                     SVPDPQTLVVTLSQSGETADTLAALHHAKSLGMKHTLCICNVPESSLVRENALRFITR
                     AGPEIGVASTKAFTTQLAALYLLTLIFAKLRGRLTEQAEATELQALRHLPVAVAKVLE
                     LEPLIREWSEHFAMKQDALFLGRGRHYPIALEGALKLKEISYIHAEAYPAGELKHGPL
                     ALVDKNMPVIAVAPNDALLEKLKSNLQEVRARGGELYVFADADGEMVAADGLHVMHLP
                     EHYGQLSALLHVVPLQLLAYHVALVRGTDVDKPRNLAKSVTVE"
     gene            complement(2104..3462)
                     /gene="glmU"
                     /locus_tag="PLD53_09635"
     CDS             complement(2104..3462)
                     /gene="glmU"
                     /locus_tag="PLD53_09635"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRC61218.1"
                     /translation="MNIVILAAGQGKRMHSNLPKVLHPVAGKALVSHVIDTARSLSPQ
                     TLCLVYGHGGDTLRNTLDAPDLAWVLQEPQLGTGHAVQQALPHLKQEGTTLILYGDVP
                     LIQSETLNRLVHAAHDALAILTVELSDPDGYGRIVRDDAGQVVRIVEQKDSTAAERSI
                     REINTGIMAMPTSRLDEWLGRLSNNNAQQEYYLTDIVGMAVEAGLPIRTANPKNEWEV
                     LGVNSKVQLAELERIAQRSTAERLMEQGVRLADPARIDVRGSLVCGRDVFIDVNCVFE
                     GQVVLDEAVEIGPNCVLTNARIGAGTRLAAFTHIEDAVVGPDNKIGPFARLRPGADLA
                     EDVHIGNFVEIKKTSIAAHSKVNHLAYIGDATIGRRVNVGAGTITCNYDGANKFQTII
                     EDDAFIGSDTQLVAPVTVGRGATLGAGTTLSEDAPPDTLTLTRPKQISISGWKRPVKI
                     KK"
     gene            complement(3622..>4343)
                     /locus_tag="PLD53_09640"
     CDS             complement(3622..>4343)
                     /locus_tag="PLD53_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61219.1"
                     /translation="SMNPELQKSLQVTPPTNTLDSYMLMILLIMVVAGILGGVANYYL
                     SDRQGEPVRRDWAKYLILGVISALTVPLFLNMLSSNLLEAARTRPVDFFVFAGFCLIY
                     VVASRRFFENVANRLLHQMDQVKREMAQIKQAAPALREEPFELPAVVSEPEVVRESLS
                     YNDIEILRELAQESYVYGNLVGLTDKTGLARDLVSSRLAVLKSLGVIETRMNERNVLH
                     WFVSPKGKQMLGDVLSGQEEH"
BASE COUNT          861 a         1249 c         1302 g          931 t
ORIGIN      
        1 ccgcaggcac tgcgcgacgg cgacctgatc gaagccgccg gctaccggct cgaattccgg
       61 cttgaaccac cagcataaag atctggtctg caagccgctt caggcggcga tctggagcgg
      121 caggcgttcg atcgtttcca gggtaacaga cacggatttc aggatagatc ggacgaacct
      181 gttgagaaac tcacaatttt gttgcctgaa cacccgcgac ggactttatt tgtctcgctc
      241 aatcatggcc gacctctgac ccgaatcact cgacagtgac cgattttgcc aaattacgcg
      301 gtttatcgac gtccgtcccg cgtaccagtg cgacgtgata ggccagcaat tgcaggggaa
      361 cgacgtgcag cagggctgag agctgaccgt aatgttctgg cagatgcatg acatgcagtc
      421 cgtcggcagc gaccatttca ccgtcggcgt cggcaaacac gtaaagctcg ccgcctcgcg
      481 ctcgaacttc ctgcaggttg gacttcagct tctccagcag tgcgtcgttg ggcgccaccg
      541 caatcaccgg catgttcttg tcgaccaatg ccagcggtcc gtgcttcagc tccccggccg
      601 gataggcttc ggcgtggatg tagctgattt ccttgagttt gagggcgcct tcaagtgcga
      661 tcgggtagtg ccgtccgcgc ccgaggaaga gggcgtcctg cttcatggca aagtgctcgg
      721 accattcgcg gatcagcggt tccagttcga gcaccttggc taccgcaacg ggcaggtgac
      781 gcaatgcctg caattcggtg gcttcggcct gctcggtgag ccggccgcgt agtttggcga
      841 agatcagggt caacaggtag agcgcggcaa gctgtgtggt gaacgctttg gtcgatgcga
      901 cgccgatttc agggccggcg cgagtgatga agcgcagcgc gttctcgcgt accagcgacg
      961 actcgggaac gttgcagatg cacagggtat gcttcatgcc gagtgatttt gcgtggtgca
     1021 gtgcggccag tgtgtcggcc gtctcacccg actgcgagag cgtgacgacc agcgtttgcg
     1081 ggtcgggaac ggattcgcgg tatcggtatt cgctcgccac ctcgacgttg cacgaaattc
     1141 cggcgatcgc ttcgagccag taacgggcga ccaggccggc atggtagctg gtgccgcagg
     1201 cgatgatcag cacctgccgt acgtctttga ggatcgactc ggtgtcagcg ccaaacagtc
     1261 cgggctggat actgcgcgcc gcgccgagca tctcaagcgt gttggatagt gcgaccggct
     1321 gttcgaagat ttccttctgc atgtaatgac gatactggcc aagctcgact gcatcggcgg
     1381 aaaggtgcga ctcggtctcc acgcgctcga ctggcgagcc gtcaacgcgg gtaatgcggt
     1441 aactgtcgcg cgacagctcg gcacagtcgc cattctccag atagacgata cggcgcgtta
     1501 cctgcagcag ggcggacgca tcggaagcgg catagcttcc gtcatcggcc aggccaagca
     1561 gcagcgggga gccttctcgg gccaccacga catgctcggg ttcggattcg cgcaggacgg
     1621 cgagggcata agcgccctgc agttgggcga tcgccgcacg cgtggcggcg aagaagtccg
     1681 agcacttctt caattcggaa gcaatgaggt ggcccaccac ctcggtgtcg gtatccgacg
     1741 tgaattcata accttcggac ttcagtcggg cacgcaagac ctcgtggttc tcgatgatgc
     1801 cgttatgtac tacggccaga ccttccgaga tatgcggatg cgcatttcgt tccgacggcg
     1861 cgccgtgcgt tgcccaacgc gtgtgagcga ttcccgtgtt gccgtacgca ctgctttgat
     1921 cagcctgtgt agtcagttcg gcgactcggc cgaccgctcg cagccgttgc aatcccgagc
     1981 cgttgatcag tgccaggcct gccgagtcat agccccggta ttcgagcttg cgcaagcctt
     2041 caagtagaac ggaaactaca ttgcggtcgg cgaccgcggc aacgatgcca cacatgatct
     2101 aactcacttt ttgattttga ccggccgttt ccagccgcta atggaaattt gcttcgggcg
     2161 cgtcagggtc agggtatcgg gaggcgcgtc ttcgctcagc gtagtgccgg cccccagggt
     2221 tgcgccgcgg ccgacagtga ccggggcaac aagctgggta tcggagccga tgaaggcatc
     2281 gtcttcgatt atcgtctgga atttgttcgc cccgtcgtaa ttgcaggtga tcgtaccggc
     2341 acccacattg acccggcggc cgatggtggc gtcgccgata taggccagat gattgacctt
     2401 ggaatgcgcc gcgatgctgg tcttcttgat ctctacgaag ttgccgatgt gtacgtcttc
     2461 cgccaggtcg gctcccggac gcaagcgagc gaagggcccg atcttgttgt ccggcccgac
     2521 gacggcatcc tcgatgtgag taaaggcagc cagacgcgtt ccggcgccga tgcgggcatt
     2581 ggtcaatacg cagttggggc cgatttcgac ggcttcgtcg agcacgactt ggccctcgaa
     2641 aacgcagttg acgtcgatga acacatcgcg cccgcagacc aaacttccgc gaacgtcgat
     2701 gcgtgccggg tcggccaggc gtacgccttg ctccatcaac cgttcggcag tgctgcgttg
     2761 ggcgatgcgt tccagctcgg ccagttgtac tttgctgtta acgccgagca cttcccattc
     2821 gtttttcggg ttggcggtac gaatcggcaa gcccgcctcg acggccatgc cgacgatgtc
     2881 ggtcaggtaa tactcttgtt gcgcgttgtt gttcgacaag cgacccagcc attcatcaag
     2941 gcgcgaggtc ggcatggcca tgatgccggt attgatttca cggatgctgc gttcagccgc
     3001 cgtcgaatcc ttctgctcga cgatacgtac aacctgcccg gcgtcgtcgc gcacgatacg
     3061 cccatagccg tccggatcgg acagctcgac agtcaggatg gccagagcgt cgtgagcagc
     3121 gtgtacgagc ctgttcagtg tttcggactg tatcaatggc acgtcaccgt acaggatgag
     3181 cgtcgtgccc tcctgtttca aatgcggcaa ggcttgttgc acggcgtgcc cggtgcctag
     3241 ttgcggctcc tgcaaaaccc aagccagatc gggtgcgtcc agcgtatttc gcaaggtatc
     3301 gccgccatga ccataaacca gacaaagcgt ctgcggcgac aggcttcgcg cagtgtcgat
     3361 gacatgcgac accagcgcct tgccggcgac cgggtgaagg actttgggca ggttggaatg
     3421 catgcgcttt ccttgtcctg cagcgagaat gactatgttc attatgggtt ctcaacaaaa
     3481 ctgactggca atctggccgc ctgtcaaatt gggccaagcg tggttcagga aaccggaagt
     3541 cggcccattt tcaccaatat ttcagtgtga acataacgac tattcacttg actcgttttg
     3601 acttctcagg tcggaagtcg attaatgctc ttcctgtccg gacaggacat cgccgagcat
     3661 ctgcttgccc ttgggtgaaa caaaccagtg caggacattt ctttcgttca tacgcgtttc
     3721 gatgacgcca aggcttttga gcacggcaag gcgcgagctg accagatcac gcgccagacc
     3781 ggtcttgtcc gtcaatccga caaggttgcc gtaaacgtaa ctctcttgcg ccaattcgcg
     3841 caggatttcg atgtcattgt aggacagcga ttccctgacc acttccggct cggatacaac
     3901 ggctggcaat tcaaatggct cttcgcgcaa ggcgggggct gcctgtttga tctgcgccat
     3961 ttcacgtttc acctgatcca tctgatgcaa aagcctgttg gccacgtttt cgaagaaacg
     4021 gcgcgaagcc accacataga tcaggcagaa acccgcaaaa acaaagaagt cgaccggccg
     4081 ggtgcgcgct gcttcgagta gattgctgga aagcatattc agaaagagcg gcacggtcag
     4141 tgcggaaatc acgccaagga tcaggtattt tgcccagtcg cgacgaacgg gttcgccttg
     4201 tcgatcactg agataatagt tggcgacacc accgaggatt ccggcaacaa ccatgatcag
     4261 cagaatcatc agcatgtagc tgtccagcgt gttggttggc ggggtgacct gcaaggattt
     4321 ctgcaattcc gggttcattg act
//
LOCUS       DAORPM010000209         4322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6235_length_4322_cov_8.323647, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000209 DAORPM010000000
VERSION     DAORPM010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6235_length_4322_cov_8.323647"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLD53_09645"
     CDS             <1..285
                     /locus_tag="PLD53_09645"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HRC61220.1"
                     /translation="KTYRVANEIGAGVGERVTIAIAAGSVRRSANLAYVLPLSATIAG
                     AVLGMFLGDDPGAMLGGMAGLLVAFLHVRRRAAAGTGFSANHPHIVSRSS"
     gene            308..1726
                     /locus_tag="PLD53_09650"
     CDS             308..1726
                     /locus_tag="PLD53_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRC61221.1"
                     /translation="MKRFLALFLFLCVSSLALAQTRGLPDFTELAEKQGPAVVNISTT
                     QVMRGNQGMPFPFDESDPAFEFFKRFIPRGPGGTTPREFENKSLGSGFIMSQDGYILT
                     NAHVVDGADEVTVRLTDKREFKAKIIGADKRTDVALLKIEASGLPAVKMADPAQLRVG
                     EWVVAIGSPFGFDNSVTAGIVSAKGRSLPQENYVPFIQTDVAINPGNSGGPLFNMRGE
                     VVGINSQIYSRSGGYMGVSFAIPIDVAMEIQNQLRVSGKVSRGRLGVVIQEVSKELAE
                     SLALSKPMGAVVNAVEKGGPADKAGLEPGDVILKFDGKPINASADLPRMVGATKPGTR
                     STLQVWRKGATRDIAVTVGEMSDEKVAASRPSRGGKPAEQQANRLGLVVSELTAEQKR
                     DLKMSSGLLIQDVRGANARADLRAGDIIIALISKGATTEVTTVEQFNKLLAQFEKGSN
                     ITLLIRRGEMQSFVTIKGLNGN"
     gene            1716..1961
                     /locus_tag="PLD53_09655"
     CDS             1716..1961
                     /locus_tag="PLD53_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRC61222.1"
                     /translation="MAIDLTLLSRSYCHLCQEMEVALAPLAEEFGASVTVLDVDSEPQ
                     LEARYDELVPVLLHGESEICHYFLDEAKTREYLAGIR"
     gene            2022..3812
                     /gene="lepA"
                     /locus_tag="PLD53_09660"
     CDS             2022..3812
                     /gene="lepA"
                     /locus_tag="PLD53_09660"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRC61223.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRIIHLCGGLSDREMEAQVLDSM
                     DIERERGITIKAQTAALQYKARDGRVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLDVEVVPVLNKIDLPSADPDNARKEIEDVIGIDATDAIL
                     ASAKTGLGVDDILEAVVARIPPPKGDPEGPLKALIIDSWFDNYVGVVMLVRVVDGVMR
                     PKDKLLFMASGAQQLCEQVGVFSPKSVSRDALRAGEVGFVIAGIKELQAAKVGDTITR
                     VDRKASEALPGFKEIKPQVFAGLYPVESNQYDSLRESLEKLKLNDASLHYEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDQDLITTAPTVVYEVVMRDGSVVQVENPAKLPE
                     LSKVEEVREPIITATIFVPQDYLGNVITLCNQKRGNQVDMHYHGRQVKLVYEIPMAEV
                     VMDFFDRLKSCSKGYASLDYEFREYRAADVVKLDILINNEKVDALSLIVHRANSQYRG
                     RELASKMRELIPRQMYDVAIQAAIGSHIVARENVKAMRKDVLAKCYGGDITRKRKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAILRVDS"
     gene            3823..>4322
                     /locus_tag="PLD53_09665"
     CDS             3823..>4322
                     /locus_tag="PLD53_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRC61224.1"
                     /translation="MNFALILFVLTLVTGVLYAVDVLKFRKLRERKYASFYGLTSKPF
                     LANPDSKFYFPSEAHLRATAYLDYGMQQNEGFVIITGESGTGKTTIIRRMLESLDPDK
                     VIAAHLVGTRGDADNTLRLVGAAFGIHTKGVEKSEVLQSLEAFLISQTSKGKRCLLIV
                     DEAQNLT"
BASE COUNT          891 a         1235 c         1333 g          863 t
ORIGIN      
        1 aaaacctatc gcgtcgccaa cgaaatcgga gccggggtcg gagagcgcgt gacgatcgcg
       61 atagccgcgg gcagcgtccg ccgcagcgcg aatttggcct atgttctgcc gttgagcgca
      121 accatcgccg gcgccgtgct cgggatgttc ctcggagacg atccgggcgc gatgcttggg
      181 ggtatggccg ggttgcttgt cgcgttcctt catgtccgtc gccgcgcggc ggcgggtacg
      241 ggtttttccg caaatcatcc ccatatcgtt tcccgttcgt cttaagcaat tcaggaggca
      301 tccgccaatg aagcgttttc tggctctctt tttgttcctt tgtgtttctt ctttggcgct
      361 tgcgcagacc cgcggtctgc cggacttcac cgagctggcc gagaaacagg ggccggccgt
      421 cgtcaacata agcacgacac aggtgatgcg cggcaaccag ggaatgcctt ttccgttcga
      481 cgagagcgac cctgccttcg aattcttcaa gcgcttcatt ccccggggac cgggaggcac
      541 cacgccgcgc gagttcgaga acaagtcgct cgggtcaggc ttcatcatga gccaggacgg
      601 ctatattctg accaatgccc atgtcgtcga cggcgcggat gaggtgacgg tgcgcctgac
      661 cgacaagcgt gaattcaagg cgaagatcat cggcgccgac aaacgcactg acgtcgcgct
      721 gctgaagatc gaggccagcg ggctcccggc cgtaaaaatg gcggatccgg cgcagctcag
      781 ggtcggcgag tgggtggttg ccatcggttc gccgttcggt ttcgacaatt cggtgacggc
      841 gggtatcgtt tcggccaagg gccgatcatt gccccaggag aactacgtcc ccttcataca
      901 gaccgatgtc gcgatcaatc ccggcaattc cggcggtccg ctgttcaaca tgcgcggcga
      961 ggtggtcggc atcaattcgc agatctacag tcgcagcggt ggctatatgg gcgtgtcctt
     1021 tgccattccg atcgacgttg ccatggaaat ccagaaccag ttgcgcgttt cgggcaaagt
     1081 cagccgcggt cgcctcggcg tggtgattca ggaagtcagc aaggaactgg cggagtcact
     1141 ggcattgagc aagccgatgg gcgccgtcgt caatgcggtc gaaaagggcg ggccggccga
     1201 caaggcggga ctcgagccgg gtgacgtgat cctcaaattc gatggcaagc cgatcaatgc
     1261 ttccgccgac ctcccgcgca tggtgggtgc aaccaagccg ggaacccgtt cgacgcttca
     1321 ggtctggcgc aagggcgcga cccgcgacat tgccgtaacc gtgggcgaga tgagcgacga
     1381 gaaggtcgct gccagtcgcc cttcccgagg tggcaagccc gcggaacagc aggccaatcg
     1441 cctcggactg gtggtgagcg aactgactgc agagcagaag cgggacctca agatgtcctc
     1501 cggcctgctg atccaggatg ttcgcggtgc caatgcgcgg gccgatctgc gtgccggcga
     1561 tatcatcatc gcgctgatca gcaagggggc aacgaccgaa gtcacgacgg tcgagcagtt
     1621 caacaagttg ctggcgcagt tcgaaaaggg cagcaacatt accctcctga tccgccgtgg
     1681 cgagatgcag tccttcgtga ccatcaaggg tctcaatggc aattgacctg actcttctca
     1741 gccggagtta ctgccacctt tgtcaagaga tggaggtggc cttggctccg ctggcggaag
     1801 aatttggtgc ttcggtgacg gttctcgatg tggattccga gccccagctc gaagccagat
     1861 atgatgaact ggtacccgtt ctgctgcatg gtgagagcga aatctgccat tactttctgg
     1921 acgaggccaa aacccgtgaa tacttggccg gaatccgcta gaattcaagg ccttctgaac
     1981 aaggaagggc gccgcaaaag cgcccttttt tactcgcatc aatggatcac atccgcaact
     2041 tctccataat cgcccatatc gaccacggaa aatcgacgct tgccgatcgc atcatccatc
     2101 tctgcggtgg actttcggac cgcgagatgg aggcccaggt gctcgattcg atggatatcg
     2161 aacgcgagcg tggcatcacc atcaaggcgc agactgcggc gctgcaatac aaggcacgtg
     2221 acggcagggt ctacaatctg aacctgatcg acacgccggg acacgttgac ttctcgtatg
     2281 aggtctcgcg ttcattgtct gcctgcgaag gcgctcttct ggttgtcgac gcctcgcaag
     2341 gtgtcgaggc gcagaccgtc gcgaactgct acacggcgct cgacctcgac gtcgaggtcg
     2401 taccggtgct gaacaagatc gaccttccct cggccgaccc cgataatgcg cgaaaggaga
     2461 tcgaggacgt gatcggcatc gacgcaaccg atgccatact tgcctcggcg aagacgggct
     2521 tgggggttga cgatatcctc gaagcggtgg ttgcacgcat tccgccaccc aaaggcgatc
     2581 cggaggggcc gctcaaggcc ttgatcatcg actcctggtt cgacaactac gtcggcgtcg
     2641 tcatgctggt gcgtgtggtt gacggggtca tgcgtccgaa ggacaagctt cttttcatgg
     2701 cgagtggcgc ccagcagctg tgcgaacagg tcggcgtatt ttccccgaag tcggtttcgc
     2761 gcgacgcact gcgcgcaggc gaagtagggt tcgtcattgc cgggatcaag gaattgcagg
     2821 cggccaaggt cggcgacacg atcaccaggg tcgaccgcaa ggcgagcgaa gccttgccgg
     2881 ggttcaagga aatcaagccg caggtgttcg ccggacttta cccggtcgaa tccaatcagt
     2941 acgattccct gcgcgaatcg ctggaaaagc tgaaactcaa cgatgcttcg ctgcactacg
     3001 agccagaggt ctcgcaggcg ctgggcttcg gtttccgctg cgggtttctg ggcctgctgc
     3061 acatggaaat cgtgcaggag cgcctggagc gcgaattcga tcaggatctg atcaccacgg
     3121 cgccgaccgt cgtctacgag gtggtcatgc gcgacggctc ggtcgtccag gtcgaaaatc
     3181 cggccaagct gccggaactg agcaaggtcg aagaggtgcg cgagccgatc atcaccgcca
     3241 cgatcttcgt gcctcaggat tatctgggga acgtcatcac gctgtgcaac cagaagcgtg
     3301 gcaatcaggt cgacatgcat taccacggcc ggcaggtaaa gctggtttac gaaattccga
     3361 tggcagaagt ggtgatggat ttcttcgacc gcttgaagtc gtgctcgaag ggctacgcgt
     3421 cgcttgacta cgagttcagg gaataccgcg ccgccgatgt ggtcaagctg gatattctca
     3481 tcaacaacga gaaggtggac gcactgtcgc tgatcgtgca tcgcgccaac tcgcagtatc
     3541 gcggccgcga actggcctcc aagatgcgcg aactgattcc ccgccagatg tacgacgtcg
     3601 cgattcaggc ggcgatcgga tcacatatcg ttgcccgcga gaacgtcaag gcgatgcgca
     3661 aggacgttct ggccaagtgt tatggcggtg acatcacacg caagagaaaa cttctggaaa
     3721 agcagaaggc cggcaagaag cgcatgaagc aggtcggcag cgtggaaatt ccgcaggagg
     3781 ccttcctggc catcctgcgt gtcgatagct gagattcaac gaatgaattt tgcccttatt
     3841 ctcttcgtgc tgacgctcgt gaccggtgtg ctgtatgcgg ttgacgtgct gaaattccgg
     3901 aaattgcgtg agcggaagta cgcgtccttc tatggcttga ccagcaagcc cttcctggca
     3961 aaccccgatt cgaagttcta ttttcccagc gaggcacatc tgcgcgccac ggcgtatctc
     4021 gattacggca tgcagcagaa tgagggcttc gtcatcatta ccggggaatc gggcacgggc
     4081 aagacgacga tcattcgcag gatgctcgaa agtctggatc cggacaaggt gatcgcggcg
     4141 cacctggtgg gcacccgggg cgatgccgac aataccttgc gtcttgtcgg cgccgcattc
     4201 ggcattcata cgaagggtgt cgagaaatcc gaggtcctcc agtcccttga agctttcctg
     4261 atcagccaga ccagcaaggg caagcgctgc ctgcttatcg tcgacgaggc gcagaatctg
     4321 ac
//
LOCUS       DAORPM010000210         4313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6256_length_4313_cov_9.871301, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000210 DAORPM010000000
VERSION     DAORPM010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6256_length_4313_cov_9.871301"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLD53_09670"
     CDS             complement(<1..292)
                     /locus_tag="PLD53_09670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HRC61225.1"
                     /translation="MKHHESPHAFTITVRIYYEDTDAGGVVYYANYLKFLERCRSEWL
                     RSIGHDQTDLLRDPGIAFVVRSINLEFLKPARLDDLLTVSLEVEKITRSQIFF"
     gene            complement(354..1442)
                     /gene="ruvB"
                     /locus_tag="PLD53_09675"
     CDS             complement(354..1442)
                     /gene="ruvB"
                     /locus_tag="PLD53_09675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRC61226.1"
                     /translation="MIEIDDFGDKLVNGVSATSDDRLISGESKSTQEDAIERALRPKR
                     LVDYVGQGKIREQLSIFIEAARRRQDTLDHVLLFGPPGLGKTTLAHIIAAEMGVNLRS
                     TSGPVLERAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGP
                     AARSVKLDLPPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTAEELTQIVTRSSQLLK
                     VLADPGGALEIARRSRGTPRVANRLLRRVRDFAEVKASGHITREVADLALLMLDVDHG
                     GLDIMDRKLLSAIIDKFEGGPVGVDNLAAAIGEARDTIEDVLEPYLIQQGFLQRTPRG
                     RIATAAIYRHLGLESPAASAQNELWRSQ"
     gene            complement(1439..2515)
                     /locus_tag="PLD53_09680"
     CDS             complement(1439..2515)
                     /locus_tag="PLD53_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRC61227.1"
                     /translation="MHQLSSAQKQIIQTSIVALLLIGCIAVLMPFTGTLLFAVVICVT
                     TAPVHNRLLALCGGRSSLAAALMSIILLVLLVAPMALLSGTLADGVETAIRYLKPVLE
                     NGLPSDPPDWLAGLPVVGSDIGDYWRKLVASREEMNTLLRQLLDPTRKLALATVSIFG
                     QGLLQLVLVVFFVFFMFRDAKTYAEALHTGSRKLAGNLGESMLKLANGTVTGVMVGIV
                     GTAAAQSLVAMVGFIIAGVPGVLMLTFATFFFSMVPVIGATLIWVGAAAWLYDGGQTG
                     WAIFMILWGMFGISSVDNFVKPILISRTSSLPLLLIVVGVFGGVLVFGFIGLFLGPTL
                     LALGQVLIREWLALTDTEAPSILS"
     gene            complement(2515..3093)
                     /gene="ruvA"
                     /locus_tag="PLD53_09685"
     CDS             complement(2515..3093)
                     /gene="ruvA"
                     /locus_tag="PLD53_09685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRC61228.1"
                     /translation="MIGRLTGILLEKTPPQLVLDVQGIGYEVDVPMSTFYNLPAIGEK
                     VTLLTHFVVREDGHFLYGFSNAAERFAFRQLIKISGVGARMALAVLSGLSVDDLAQAI
                     ARQEVGRLIKVPGIGRKTAERLLLELKGKLAEALPSASATAPENNQHDILNALLALGY
                     NDREATAAGKQLPAGISTSDGIRQALKLLSRL"
     gene            complement(3090..3701)
                     /gene="ruvC"
                     /locus_tag="PLD53_09690"
     CDS             complement(3090..3701)
                     /gene="ruvC"
                     /locus_tag="PLD53_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRC61229.1"
                     /translation="MNPRHERIALIQKANCPIRILGIDPGLRITGFGVIEMSGNTLTY
                     VASGCIKTDAKTSLPTRLGAIHSGLRELVAQYQPDQAIVEIVFVNANPQSTLLLGQAR
                     GAAITALVADGLEVAEYTALQVKQAVVGNGHADKAQVQHMVRRLLALPGDISADAADA
                     LACAITHAHGGQGLGHGAAALATKGFRVRNGRMIMGKQKESEK"
     gene            complement(3928..>4313)
                     /locus_tag="PLD53_09695"
     CDS             complement(3928..>4313)
                     /locus_tag="PLD53_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC61230.1"
                     /translation="TLYQKRFCPRFDLRTGSVIQFLPAAVLTAIAAGATESMSIDWTP
                     HFVFALLWLVLVLSFGAISLLNVLIRSGSAVNVARLFYLTPPSTAIIAWVLFGETLSG
                     LALAGMVLAVGGVYMAGETAKPAKT"
BASE COUNT          942 a         1275 c         1267 g          829 t
ORIGIN      
        1 gaaaaaatat ctgggaccgg gtaatctttt cgacttcgag actgaccgtc agaaggtcgt
       61 caagccgcgc cggcttcaga aactcaaggt ttatgctgcg tacgacaaat gcaattccgg
      121 ggtcgcgcag cagatcggtc tggtcgtgcc cgatcgagcg cagccattcg ctgcggcagc
      181 gctccagaaa cttcaggtaa ttggcgtaat aaaccacgcc gccggcgtcg gtatcttcgt
      241 agtagatgcg cacggtaatg gtgaatgcat gggggctttc gtggtgtttc atgagcggat
      301 tctattacag acagattgcg tcgagtgtat gcccatgcac ctgagccacc tgctcactga
      361 gaccgccaga gttcgttctg cgcgctggcc gcggggctct cgaggccgag gtgacggtaa
      421 atggctgccg tggcaatacg tccgcgcggc gtgcgctgca ggaatccctg ctggatgaga
      481 tagggttcga gcacgtcttc gatcgtatcg cgcgcttcgc caattgcagc ggcgagatta
      541 tcgacaccga ccggcccgcc ttcgaacttg tcgatgatgg cggaaaggag tttgcggtcc
      601 atgatgtcga gaccgccgtg atcgacatcg agcatgagta gtgccagatc ggccacttca
      661 cgggtaatgt gtcctgaagc cttgacctcg gcgaagtcgc gcacgcgacg cagaaggcga
      721 ttggcgacgc gcggtgtgcc gcgggaacgc ctggcgatct ccagcgcgcc gcctggatcg
      781 gccagcactt tgagcagttg cgacgaacgc gtaacgatct gcgtcagctc ctccgcagta
      841 taaaactcga ggcgcgcgac gatgccaaaa cgatcgcgca gcgggttggt cagcattccc
      901 gcgcgggtcg tcgcgcccac cagtgtaaag ggaggtagat caagctttac cgagcgtgcc
      961 gcggggccct cgccgatcat gatgtcgatc tggaaatctt cgagcgcggg gtagagaatt
     1021 tcttcgacaa ccggactcaa acggtgaatc tcatcgatga acagcacatc atgcggttcg
     1081 agattggtca gcatggcggc cagatcgccg gcacgctcca gcaccgggcc cgaggtcgag
     1141 cgcaggttga cgcccatctc ggcagcgatg atgtgcgcca gtgtggtttt ccccagtccg
     1201 ggcggaccaa acaacagcac gtgatcgagc gtatcctggc gcctcctggc cgcttcgatg
     1261 aagatggaca actgttctcg aatcttgccc tggcccacgt aatcgaccag ccgtttgggg
     1321 cgtagtgcgc gctcgatcgc atcttcctgc gtcgatttgg actctccgga aatcaggcga
     1381 tcatcggatg tcgcgctcac accgttaacc agcttgtcgc caaagtcgtc tatttcaatc
     1441 atgacaaaat cgatggcgct tcagtatcgg tgagagccag ccattcccgg atcagtacct
     1501 gaccaagcgc gagcaatgtc ggtcccagaa agagtccgat gaatccgaaa accagcacgc
     1561 caccaaagac gccgacaacg atcaacaata gcggcaaact ggaggtgcgc gagatcagga
     1621 tcggtttgac aaaattgtcg acgctgctga tgccgaacat gccccacaga atcatgaaaa
     1681 tggcccagcc cgtttgtccg ccatcgtaga gccacgcagc cgccccaacc cagatcaagg
     1741 ttgcgccgat cactggcacc atcgagaaga agaacgtggc aaaggtgagc atgagcacgc
     1801 cgggaacacc ggcgatgatg aaaccgacca tggcgaccag cgactgcgcg gcggcggtac
     1861 cgacgatgcc aaccatcact ccggtgacgg tgccattggc cagtttgagc atgctctcgc
     1921 ccaggttgcc cgccagcttt cggctaccgg tgtgtagcgc ttccgcatat gtttttgcgt
     1981 cgcggaacat gaagaacacg aagaagacca ccaggaccaa ttgcagcaat ccctgcccaa
     2041 aaatcgacac ggtggccagg gcaagcttgc gcgtgggatc gagaagctga cgcagaagtg
     2101 tgttcatctc ctcacggctg gcaaccagct tgcgccagta atcgccgatg tccgaaccga
     2161 caacaggcag accggcaagc cagtctggtg gatcgctggg tagtccgttt tcaagaacgg
     2221 gcttcaagta gcgaatggcc gtttcaacac cgtcagcgag cgtgccggat agcagggcca
     2281 ttggagcgac cagcaacacc agcagaatga ttgacatcag cgcggcggcc aggctgctgc
     2341 gaccgccaca aagcgcgaga aggcggttgt gaaccggcgc tgtcgtaacg caaatcacga
     2401 cggcaaaaag cagtgtgccg gtgaacggca tcagtacagc gatacaaccg atcagcagaa
     2461 gcgcgacaat ggaagtctga atgatctgct tctgggctga agataattga tgcatcataa
     2521 tcgtgagagt agtttgagcg cctgacgaat gccatcggaa gtcgaaatgc cagcgggtag
     2581 ctgcttgcct gcggcggtcg cttcgcggtc attgtaaccc agcgccagca gcgcattgag
     2641 gatgtcatgc tggttgtttt ccggagcagt ggccgaagcc gaaggcaagg cctcggccaa
     2701 tttgcccttg agttcgagca gcagacgttc ggctgtcttc ctgccaattc ccggcacctt
     2761 gatcagccgc ccgacctcct gtcgtgcgat ggcttgtgcc agatcgtcga ctgacagccc
     2821 ggatagcaca gccagtgcca tgcgcgcgcc aacccccgat attttgatca gctggcgaaa
     2881 cgcgaaacgc tccgcggcgt tggagaagcc gtagagaaag tgtccgtctt cgcgcaccac
     2941 aaaatgcgta agcagggtca ccttctcgcc gattgccggc agattgtaaa aggtactcat
     3001 cggcacatcg acctcgtagc cgatgccctg cacatcgaga acaagctgcg gcggagtttt
     3061 ttccagcaat attccggtca ggcggccgat catttttcgc tctctttctg cttgcccata
     3121 atcatgcgcc cgttgcggac ccggaacccc ttggtggcca gcgctgccgc cccgtgaccc
     3181 aggccctggc caccatgcgc atgggtgatc gcacaagcca gcgcgtcggc agcgtccgcg
     3241 ctgatgtcgc cgggcagtgc caacagccga cgcaccatgt gctgcacctg cgccttgtcg
     3301 gcgtggccgt tgccgaccac cgcctgtttg acctgcaacg ccgtatattc ggcgacctca
     3361 agcccgtcgg caaccagcgc cgtgatggct gccccgcgtg cctggccaag aagcagtgtc
     3421 gactgcggat tggcgttcac aaagacgatt tcgacgatcg cttgatccgg ttgatactgc
     3481 gcgaccagct cgcgcaggcc gctatgaatt gctcccagcc gcgtcggtaa agacgttttg
     3541 gcatccgtct tgatgcaccc gctggccaca taggtcaacg tgtttccaga catttcgatg
     3601 acgccgaatc cggtgatgcg cagaccgggg tcaatgccga ggattcggat cggacaattt
     3661 gctttctgga tcaatgcgat gcgctcgtgt cgggggttca ctggacagcg cacagtataa
     3721 cgagtcgtag cctgttgatc cggccgtatc gaaagttgtg cggatgatcg catcacccgg
     3781 agccaccatt ctggataacc gctcggagcc gagcagcgtt caatatgcag cgataagacg
     3841 ccatcggctt gagccgggct cttttcgcgg ctgataccga ttgcgagtct gtctgccgtg
     3901 cgttcgttta aacgggtcaa gcttccttca ggtcttcgcc ggcttggctg tttcaccggc
     3961 catatacaca ccgcccaccg ccagaaccat accggccaaa gccaaaccgc tcagcgtttc
     4021 gccgaacaac acccaggcaa tgatggcggt gctcggcggc gtcagataga acagccgcgc
     4081 gacattgacc gcactgccac ttcgaatcag cacattgagc aggctgatcg caccgaacga
     4141 gagaaccagc accagccaga gcaaagcgaa gacgaaatgc ggagtccagt cgatactcat
     4201 gctttcggtt gctccggccg caatcgcggt gagcaccgct gccggcaaaa actggatcac
     4261 cgaaccggta cgcaggtcga aacgcgggca gaagcgtttc tgatacaggg ttc
//
LOCUS       DAORPM010000211         4294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6288_length_4294_cov_8.945270, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000211 DAORPM010000000
VERSION     DAORPM010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6288_length_4294_cov_8.945270"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLD53_09700"
     CDS             <1..608
                     /locus_tag="PLD53_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC61231.1"
                     /translation="AQDLSEMRIDTSFAEADIGRIKAGQKVRFTVDAFPSRTFEGEVQ
                     QIRLNATTLQNVVTYNVRVSLSNPEQILLPGMTAYVNIAVDHRQDALLVPNAALRFKP
                     SDADAIDKDKTETAASESRPGGPGNGGGKPGRKKGDTANGMVYVISDGAPKAISVQLG
                     ITDNRNTEVVGGALKAGDRVITGEISGNSGGKPSSIGMRMF"
     gene            608..1318
                     /locus_tag="PLD53_09705"
     CDS             608..1318
                     /locus_tag="PLD53_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC61232.1"
                     /translation="MADAVVRVSGLGKTYATAAGLFPALKGVDLVIDSGEFVAIMGPS
                     GSGKSTFMNLLGCLDTPSSGSYHLVGRNVAQLDSDELSALRNRTIGFVFQGFNLLPRM
                     NLEDNVALPLVYSGVEREVRREKGRQMLARVGLESYAKSLPSRISGGQQQRVAIARAL
                     VNSPQLILADEPTGNLDSHTGEEIMSLFEALNQEGITIVLVTHESDIAAHAKRQVRFL
                     DGHIVSDLATSRREELAC"
     gene            1312..2544
                     /locus_tag="PLD53_09710"
     CDS             1312..2544
                     /locus_tag="PLD53_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC61233.1"
                     /translation="MLGNMLGAMLGEAWHAMGANRLRTILTMLGMVIGVGSVVLMMAI
                     GQGAQYAVAQTISTMGSNLYMVVSGSTSAAGVRSGSGAGPTLNVSDADAIAELDAVTY
                     AAPIHYGRQQLVFGSNNWNSTVVGTTPAYLGARSWTIVSGYGFSESDVRSASRVALIG
                     QTAAENLFGADDPIGKTIRIQQSPFTIVGVLGSKGQNLDGRDQDDTVIIPLTTAQRKV
                     FGTPFQGSVRVIMVQAASADAMPVVEKSVTALLRQRHRLREDMNNDFYIRNLAAAADS
                     AAATTRVMSLLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRLAIGARQKDILTQ
                     FLLEALMISVAGCLIGLILGIGGALLINAITNMVIVISGGSVLVAFAVAAGVGVFFGF
                     YPARRAAALDPIEALRYQ"
     gene            2570..3091
                     /locus_tag="PLD53_09715"
     CDS             2570..3091
                     /locus_tag="PLD53_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRC61234.1"
                     /translation="MPIYKLGNHTPQIDPQCWIAPNATVIGQVHLARNVSIWWNSTLR
                     GDKDLISIGENSNIQDGSVLHTDPGIHLTVGRNVTVGHMVMLHGCTVGDGALIGIGSI
                     LLNRSVIGKHSLVGANSLIPEGKVFPERVLIVGSPGKVVRELTDEEVAGLPGSAERYV
                     ENWQRYREELNSI"
     gene            3162..>4294
                     /locus_tag="PLD53_09720"
     CDS             3162..>4294
                     /locus_tag="PLD53_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRC61235.1"
                     /translation="MIKMLKNTALIITLLIFAGQALAEIVIGQVTPLSGPLAPTGSHI
                     RAGAQLYFDAVNAKGGVHGEKIRLVTKDDAYKAAETVRLAKELINEVQPLALFGFVGT
                     GNAEAILKDKILSNAGIPLVMIRSGASAVVKSGNPWLFITRASYEAEINKIIQQYSLT
                     GYRTYAVLYQDDPFGLDVLGSTEALVGKFDSKIVAKAGYKKNTTEVSEAVKTIAAAAP
                     HAVIMISNTAASSEFLKQSRAAGNNAQFITISVTDAAQVVERIGKDVANGLALTQVFP
                     DPESRSIPLTREIMENFSKFKPSGEITPNQTLVEGYVGAKVLVEGLRRAGPKPTRQKL
                     RDALEQIKDYDTGDLYVGFSPTNHSGVHYVDITIINREGKLLK"
BASE COUNT          948 a         1271 c         1220 g          855 t
ORIGIN      
        1 ttgcccagga tctctccgaa atgcgcatcg acacgagctt tgcggaagcc gacattggca
       61 ggatcaaggc gggccagaag gttcgcttta ccgtcgatgc ttttccaagc cgaacttttg
      121 agggcgaagt gcaacagatc cgcctcaatg cgacgacact gcaaaacgtc gtaacctaca
      181 atgttcgcgt atcgctctcc aaccccgaac agattctgct gccaggcatg acggcctacg
      241 tgaatatcgc agtggatcat cgccaggatg cgttgctggt gccaaacgct gcactgcgtt
      301 tcaaaccatc cgatgccgat gccattgaca aggacaagac tgaaacagcg gcttctgaaa
      361 gtcgccccgg cgggccgggc aacgggggcg ggaagccagg ccggaagaaa ggcgacacgg
      421 ccaacggtat ggtctatgtc atcagcgacg gggcgccgaa agcgatcagc gttcaactgg
      481 gcatcaccga caaccgaaat accgaagtcg ttggcggcgc tctcaaggcg ggcgaccgcg
      541 tgattaccgg cgagatcagc ggcaactcgg gtggcaagcc aagcagcatc ggcatgagga
      601 tgttctgatg gccgacgccg tcgttcgtgt ttccggtctt ggcaaaacgt acgcgacggc
      661 ggccgggctg tttccagcac tcaagggcgt cgatctggtc atcgatagcg gcgagttcgt
      721 tgccatcatg gggccgtcgg gatcgggaaa atccacattc atgaacctgc tcggctgcct
      781 cgacacgcca agcagcggct cctatcacct ggtcggccgg aacgtcgcac aactggacag
      841 cgatgaactg tcggcgcttc gcaaccggac catcggtttt gtctttcagg ggttcaatct
      901 gctcccgcgc atgaatcttg aagacaacgt ggctctgccg ctggtttact ccggcgtcga
      961 gcgcgaagtg cggcgtgaga agggccgaca aatgctcgcc cgcgtcggac tcgaaagcta
     1021 tgcaaaatca ctgccttcgc gcatctcggg cgggcagcaa cagcgcgtcg ccatcgcccg
     1081 cgcgctggtc aattcgccac aactgatact cgccgacgaa ccgaccggca atctggacag
     1141 ccataccggc gaagaaatca tgtcgctttt cgaagcgttg aaccaggaag gcatcaccat
     1201 cgtactggtc actcacgaat ccgatattgc ggcgcatgcc aagcgccagg tgcgctttct
     1261 cgacggacac atagtcagcg atctggctac ctcgcgacgg gaagagctag cgtgttaggc
     1321 aacatgctcg gcgcgatgct tggcgaggcc tggcatgcca tgggcgccaa tcgcctgcga
     1381 acgatactga ccatgctcgg catggtgatc ggtgtcggtt cggtggtcct gatgatggcg
     1441 atcggccagg gcgcgcaata cgccgttgcg caaaccatca gcaccatggg cagcaacctg
     1501 tacatggtgg tttccgggtc aaccagcgct gccggcgtgc gtagcggcag tggcgccgga
     1561 ccgaccctca atgtctccga cgccgatgcc atcgccgagc ttgatgccgt cacgtatgca
     1621 gcaccaatcc actacggcag gcaacagctt gtcttcgggt cgaacaactg gaattcaacg
     1681 gtcgtcggca ccacgcctgc ctacctggga gcgcgttcct ggacaatcgt cagcggttac
     1741 ggtttcagcg aaagcgatgt ccgctcggcc tcgcgtgttg cgctgatcgg gcaaaccgca
     1801 gccgaaaatc tgttcggcgc cgatgatcca atcggcaaga caatccgtat ccagcaaagc
     1861 cccttcacca tcgttggcgt gctgggcagc aaggggcaga atctcgatgg ccgcgatcag
     1921 gacgacacgg taatcattcc gctcaccacg gcgcagcgca aggtcttcgg cactccgttc
     1981 cagggatcgg tgcgcgtgat catggttcag gccgcctcag cggatgcgat gccagtggtc
     2041 gaaaaatcgg tgacggccct gcttcgtcag cgccaccgtc ttcgtgaaga catgaacaac
     2101 gatttctaca ttcgcaatct ggcggcggcc gccgattccg ctgcagccac cacacgcgtg
     2161 atgtccctgc tgctcggcgc aatcgcttcg gtatcgctgc tcgtcggcgg catcgggatc
     2221 atgaacatca tgcttgtttc ggttaccgaa cgaactcgcg aaatcggcat tcgcctggcc
     2281 attggcgctc gccagaagga catcctgacg cagttcctgc tggaagctct gatgatttcc
     2341 gtcgccggat gcctgatcgg cctgattctt ggcatcggag gcgcgctgct aatcaacgcc
     2401 atcaccaaca tggtgatcgt tatttcaggc ggctcagtac tcgtcgcctt tgcggttgcc
     2461 gccggagttg gcgtattctt cggattctat cccgcacgtc gggcagccgc gctcgatccg
     2521 atcgaggcgc tgcgctatca gtaacccaac ccgtaacaac actaacaaca tgcctatcta
     2581 caaacttgga aaccacaccc cgcaaatcga cccgcaatgc tggatcgccc ctaatgcaac
     2641 ggttatcggc caggtgcatc tggcgcgaaa cgtctcgatc tggtggaact cgacgctgcg
     2701 cggcgacaag gacctgatca gcatcggcga gaacagcaat attcaggatg gatcggtgct
     2761 gcacaccgac cccggcatcc atcttaccgt gggccgaaat gtcacggtcg gtcacatggt
     2821 gatgctgcat ggctgcaccg ttggggatgg ggcgctgatc ggcatcggct cgatcctgct
     2881 caatcgttcg gtgatcggga aacactcgct ggtgggcgcc aactcgctga ttccagaagg
     2941 caaggtgttc ccggagcgcg ttctgatcgt tggctctcct ggcaaggttg tccgcgaact
     3001 gacggacgag gaagtcgctg gccttcccgg aagcgcagag cgctatgttg aaaactggca
     3061 gcgctatcgg gaggaactga attccatttg acggcttctt gacttcgcat tcccaacggc
     3121 atagattcag tccgttcatc ccctcaggag aaggttcgac aatgataaaa atgctcaaaa
     3181 acacggctct cattatcacc ttgctgatct ttgctgggca ggcactggcc gaaatcgtca
     3241 tcggtcaggt taccccactc tccggcccgc tcgctccaac gggtagccac attcgcgccg
     3301 gcgcacagct ttacttcgac gccgtcaatg cgaaaggtgg cgtacatggc gaaaagattc
     3361 gtctggttac aaaggatgat gcctacaaag ccgccgaaac cgtgcgattg gccaaggagt
     3421 tgataaatga agtgcagcct cttgcactct tcggcttcgt cggaacaggc aatgcagagg
     3481 ccatcctgaa ggacaagatt ctgtcaaatg ctggcatccc cctggtcatg atccgcagtg
     3541 gcgcatctgc ggtagtgaag agcggcaacc cgtggctgtt cattacacga gccagttatg
     3601 aggcagagat aaacaagatc atccagcaat acagtctcac cggttaccgc acctatgcgg
     3661 ttctctacca ggatgacccg tttggtctcg acgtgctggg tagcaccgaa gcattggttg
     3721 gcaagttcga cagcaagatt gtcgccaaag ccgggtacaa aaagaacact acagaggttt
     3781 ccgaagcggt caaaacaatt gccgccgccg caccgcatgc tgtgatcatg atctcgaaca
     3841 cggcggcgag ctccgaattt ctcaaacagt cgcgggcggc gggaaacaat gctcaattca
     3901 ttacgatttc cgttactgat gccgcgcagg ttgtagagcg catcggaaaa gatgtggcta
     3961 acgggcttgc cctcacccag gtattcccgg acccggaaag ccggtccatt ccgctgacgc
     4021 gggaaataat ggaaaatttt tccaaattca aaccttctgg cgaaataacc cccaaccaga
     4081 ccctggtgga gggctatgtt ggcgccaaag tcctagtcga aggcttgcga cgtgccggtc
     4141 caaaaccgac tcgccaaaag cttcgcgacg ccctcgaaca gatcaaggat tatgacaccg
     4201 gcgacctgta tgtcggtttc tccccgacca accattccgg ggtgcactac gtcgacatca
     4261 ccatcatcaa tcgggaaggc aagcttctga agta
//
LOCUS       DAORPM010000212         4285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6312_length_4285_cov_9.933806, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000212 DAORPM010000000
VERSION     DAORPM010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6312_length_4285_cov_9.933806"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLD53_09725"
     CDS             complement(<1..770)
                     /locus_tag="PLD53_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRC61236.1"
                     /translation="MQATMGAADRLAQVGAVAIRDHMPRQHREFFSLLPFLVVGSIDQ
                     SGQPTASILAGPPGFVQTPDERHLRVAALPLAYDPLDLNLQSGAPLGILGIQPHTRRR
                     NRVNGRVTLLDEHGFTLAVEQSFGNCPKYIHAREPSYVQQASEAKLICDEGLPDSARV
                     MIAQADTCFIASAHPDALVADDRSHGVDVSHRGGPSGFIRIMPDDTLMLPDFPGNRFF
                     NTFGNLALNPFAGIMILDYVAGDVWQFDVSCQIVTDPD"
     gene            complement(821..1450)
                     /locus_tag="PLD53_09730"
     CDS             complement(821..1450)
                     /locus_tag="PLD53_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRC61237.1"
                     /translation="MSSKQPDQPIRFHSFPYSGHCHRVALLLSDLQLPHETINVDLAN
                     KAQKSEAFLAMNPFGQVPVIQDGDVTIADSLAILTYLSERYGVGRWAPRTPIEAAEIQ
                     RWFALAAGTLAFGPAAARASKLFNAPVDLPAAQARSLALFDVVDRHLAQRDYLAGQNL
                     TLADLAFYAYVARAPEGGVSLDPYSHIRSWLARVESMPRFVPMPEFPKR"
     gene            1559..2512
                     /locus_tag="PLD53_09735"
     CDS             1559..2512
                     /locus_tag="PLD53_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRC61238.1"
                     /translation="MDKLKAMQTFVAIADAGSLTAAAEVLNSSLPAVVRGLAQLEESL
                     GVRLFNRTTRRISITEEGRRYLERSRQILSLTAEAEAELRDEEAEPQGVLRITAPVLF
                     GQLHVCDAITRFVQRYPKVSVDVQLLDRVVNLIEEGFDLGVRIGELENSTLVAQGLGH
                     LRRVVVAAPAYLEQHGTPLHPRELLNHNCLRFIGSRAPWWTFYDRGKVFTLPVQGSLT
                     FNQAAPVARACLAGLGFGMFISYQVAPHLRSGALKIVLADYEPPPRPIHLLYPQSRLL
                     PARTRALINWLKSEIGPIRFDGKFGEEERSAAILNKTQDQG"
     gene            complement(2579..3799)
                     /locus_tag="PLD53_09740"
     CDS             complement(2579..3799)
                     /locus_tag="PLD53_09740"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HRC61239.1"
                     /translation="MQIFTVGGAVRDELLGLPVQDRDYVVVGATPEEMLARGFKPVGK
                     DFPVFLHPKTHEEHALARTERKTGQGYQGFSFHAAPEVGLEEDLARRDLTINAIARAD
                     DGSLTDPFHGVADIEARVLRHVGPAFIEDPVRILRVARFAARFTDFSVAPETVELMRQ
                     MVVRGEADHLVAERVWQELAKGLMEAKPSRMIYMLRECGALARLLPELDRLFGVPQRA
                     DFHPEIDTGVHVMMALDHSAQQGYGLPVRFAVLLHDLGKGLTPATELPRHVGHEARSV
                     MLVEELCARLRTPADCRDLALLVARHHGDIRRGPEMSAATVTKLLENADALRRPHRFR
                     QLLDACACDFHGRLGWEEKPIPSPELFLIALDAIRAIDAAAIARSCKDASQIPQRLYD
                     ARVVAVKKALELPD"
     gene            complement(3938..>4285)
                     /locus_tag="PLD53_09745"
     CDS             complement(3938..>4285)
                     /locus_tag="PLD53_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HRC61240.1"
                     /translation="LTDRASFGQIYELCGPKVYTLRELVEYTGNLIGRRRAIIDLPNG
                     PANLLARIMGLLPNPPMSPDNLRSMEKDNVTDGLRNYPGWQPRSLEAEAPGYLSAVRP
                     KMRYDNYRCRAGR"
BASE COUNT          909 a         1237 c         1288 g          851 t
ORIGIN      
        1 tgatcgggat ccgtgacgat ctggcatgac acatcgaatt gccagacatc gcctgcgacg
       61 tagtccaaga tcatgattcc ggcaaacggg ttgagggcaa gattgccgaa cgtattgaag
      121 aatcgattcc ctggaaaatc cgggagcatc aatgtgtcgt ctggcatgat cctgataaat
      181 ccggaggggc cacctcgatg cgaaacgtca acgccatgtg aacggtcgtc agcgaccaac
      241 gcatccggat gcgcgctcgc gataaagcag gtatctgcct gcgcaatcat gacgcgggca
      301 ctatctggca gcccttcatc gcagatcagt ttcgcttccg acgcctgttg cacatagctc
      361 ggctcacgag cgtgaatata tttcgggcag ttcccaaagc tctgttcaac ggccagggta
      421 aaaccatgtt cgtccagtag agtgacgcgg ccattgacgc gattgcggcg gcgcgtatgc
      481 ggttgaatcc ccaggatgcc aagcggcgcg ccactctgca ggttgagatc cagtgggtcg
      541 tatgcgagag gcagcgcagc gacacgcagg tgccgttcat ccggtgtctg cacgaatccg
      601 ggtgggccag ccagaattga ggcggttggt tgcccggact gatcaatcga cccgactacc
      661 agaaacggca gcaacgaaaa gaattcgcgg tgttgccgcg gcatatggtc gcgtatcgca
      721 actgccccca cctgcgcaag tcggtccgcc gcgcccattg tggcttgcat ggcgcgttcg
      781 ccatggtgga atgccccatt cggttccatt gctggctagc tcaacgcttc ggaaattccg
      841 gcatcgggac gaagcgcggc atggattcga cccgcgcaag ccaactgcgg atatgcgagt
      901 acggatcaag cgaaacgccg ccttccggtg cgcgcgcgac ataggcgtag aaagcgagat
      961 cggcgagcgt caggttctgc ccggccagat agtcgcgctg cgccagatgc cggtccacta
     1021 catcgaatag tgcgaggctg cgtgcctgag cggcaggtag gtcgaccggt gcattgaaga
     1081 gcttgcttgc gcgcgctgcc gccggaccaa aagcgagagt cccggcggcc agtgcgaacc
     1141 aacgctggat ttcggcggct tcaattggag tgcgcggcgc ccaacgccct acgccataac
     1201 gttcggaaag gtaggtaagg atggccagcg aatcggcaat ggtcacatcg ccgtcctgga
     1261 tcacgggcac ctggccaaag gggttcatgg ccaggaacgc ctccgatttc tgcgctttat
     1321 tggccagatc gacattgatc gtttcgtgcg gaagttgcag gtccgacaac agcagagcca
     1381 cgcggtggca gtgtccggaa tagggaaagc tgtggaaacg gatgggttga tctggctgct
     1441 tgctggacat gatgggaccc tcggtatgga agaaggagtc atcgtcgtct ttcaatgaag
     1501 ctgaataaat acctaaacaa gtattaaact aatccgattt gtggagcaat cggcccgaat
     1561 ggacaagctg aaagcgatgc aaaccttcgt cgcgatcgcc gatgcaggta gcctgactgc
     1621 ggcggctgaa gtcctcaaca gttcattgcc agccgttgtg cgcggtttgg ctcagctgga
     1681 ggaaagtctt ggtgtgcgtc tcttcaaccg cactacgcgc cgtatctcca ttaccgaaga
     1741 gggtcgacgc tatctcgaac ggagccgcca gatactgagt ctgacggccg aggccgaagc
     1801 tgaattgcgt gacgaggaag ccgagcctca aggcgtgctg cgcatcacgg cacccgtgct
     1861 cttcggacaa ttgcatgttt gcgatgccat cacgcgcttt gttcagcgct acccgaaagt
     1921 gagtgtcgat gtgcagttgc tggatcgcgt ggtcaatctg atcgaagaag gattcgatct
     1981 cggtgtgcgc attggcgaac tggagaattc gaccctggtt gcacaagggc tcggtcatct
     2041 gcgccgtgtc gtcgtcgccg ctccggccta ccttgaacaa catggcactc cgctgcatcc
     2101 ccgtgagttg ctcaaccaca actgccttcg attcattggt agcagagcgc cctggtggac
     2161 cttctacgat cgggggaagg ttttcacgct gcccgtccag ggatcgctga cgttcaacca
     2221 ggctgcaccg gtcgccaggg cttgtctggc cgggctcggc ttcggcatgt tcatttctta
     2281 ccaggttgcg ccgcacttgc ggtcaggcgc gctgaagatt gttttggccg actacgaacc
     2341 gccaccccgg ccgattcacc ttctgtatcc acagtcgcgg ctgctgccgg cacgaacacg
     2401 agcgctgatc aactggctga aatcggaaat cggacctata cgtttcgacg gcaagttcgg
     2461 cgaagaagaa cgttcggcag cgatcttgaa caagacgcaa gatcaagggt aaccagcgtc
     2521 ttcccgaatc gccaaatcac acgtggtcaa ccggtcggcg cttgatcctt gcaccgaatc
     2581 aatccgggag ttccaaagcc ttctttactg cgaccacccg tgcatcgtac aagcgctggg
     2641 gaatctggga cgcgtccttg caactgcgcg caatggcggc tgcgtcaatg gcgcgaatcg
     2701 cgtccagcgc aatcaggaac aactccggcg acgggatcgg cttttcttcc cagccgagtc
     2761 ggccatgaaa atcgcaggca cacgcatcga gaagttgtct gaaacgatgc ggacggcgca
     2821 gcgcatcggc gttttccagg agcttggtaa cggtagctgc gctcatttca ggaccacggc
     2881 gaatatcgcc atgatgacgc gcaaccagga gtgccagatc gcgacagtct gccggagtcc
     2941 tgagtcttgc acacaattcc tcgaccagca tgacgctgcg cgcctcgtgc cccacatggc
     3001 gtggcagttc ggtcgccggt gtcagcccct tgccgagatc gtgcaaaagt acggcaaagc
     3061 gtaccggcaa gccatagccc tgctgtgcgg aatgatcgag tgccatcatg acatgcacgc
     3121 cggtatcgat ttcaggatgg aaatcggcac gctgcggcac gccgaacaaa cgatcaagct
     3181 cgggtagcag gcgcgccagt gcaccgcatt cgcgcagcat atagatcatg cgcgagggtt
     3241 ttgcttccat cagccctttg gccagttcct gccagacgcg ttcggcaacc agatgatccg
     3301 cctcgcctct gacgaccatc tggcgcatca gctcgacagt ctccggggca actgaaaaat
     3361 cggtgaagcg cgcggcaaat cgggcgacgc gcagaatgcg taccgggtcc tcaatgaagg
     3421 ccggtccgac atggcgcagt acccgcgctt cgatgtcggc cacaccgtga aacgggtcag
     3481 tcagtgaacc atcgtcggcg cgagcaatag cattgatggt caggtcgcga cgagccaggt
     3541 cttcttcgag tccgacctcg ggagcagcat gaaaggagaa gccctggtac ccttggccgg
     3601 tcttgcgctc agtacgggca agcgcgtgct cctcgtgcgt tttcgggtgc aagaatacgg
     3661 ggaagtcctt gccaaccggc ttgaagccgc gcgccagcat ttcctcgggc gtcgcgccaa
     3721 cgacgacata gtcgcggtcc tgcaccggca agcccagcaa ttcgtcgcgg accgcgccgc
     3781 caaccgtgaa gatttgcata agggaaagag ggggcagtta gcccctctca gtcggcgcat
     3841 ccgctcgcca ttctggtctg ccgggaaatg cgtatcgggc agcgtggttg tgttcgccga
     3901 tgacaggttt cagcttgaca ggcattgcct gaaattctta ccgacctgca cgacaacgat
     3961 aattgtcata gcgcatcttt ggtctgacag cggaaagata gccaggcgcc tctgcttcaa
     4021 gtgagcgcgg ttgccaaccc gggtaattgc ggagaccgtc ggtaacgttg tccttttcca
     4081 tggaacgcag gttatccgga gacatcggcg gattgggaag cagccccatg attcttgcca
     4141 gcagattggc cgggccattg ggcaagtcaa taatcgcgcg gcgccggccg atcagattgc
     4201 cggtgtactc gaccaattcg cgcaatgtgt agaccttcgg gccgcacaac tcgtagatct
     4261 ggccaaagct ggcccgatcc gtcag
//
LOCUS       DAORPM010000213         4277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6337_length_4277_cov_21.987684, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000213 DAORPM010000000
VERSION     DAORPM010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6337_length_4277_cov_21.987684"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLD53_09750"
     CDS             complement(<1..692)
                     /locus_tag="PLD53_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRC61241.1"
                     /translation="MSTNLPLSAESVELPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     MAMEAGKSILLVAQKSAVKDEPEAEDLYSVGCVANILQMLKLPDGTVKVLVEGTQRAQ
                     VHSIEARDEMFVAIAQPLAVEDGVDHEVEGLRRAVISQFDQYVKLNKKIPPEILTSIA
                     SIEEAGRLADTIAAHLPLKIEQKQEVLEMFEIRPRIERLLSQLETEIDILQVEKRIRG
                     RVKRQMEKSQRE"
     gene            complement(776..2047)
                     /gene="clpX"
                     /locus_tag="PLD53_09755"
     CDS             complement(776..2047)
                     /gene="clpX"
                     /locus_tag="PLD53_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRC61242.1"
                     /translation="MTEKKSGSEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIELCN
                     DIVRDEISADLGDKVAKSDLPTPREISGLLDQYVIGQDSAKRILSVAVYNHYKRLRHR
                     GKVNDDIELAKSNILLIGPTGSGKTLLAQTLARMLNVPFVMADATTLTEAGYVGEDVE
                     NIIQKLLQKCDYDTDKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEG
                     TVASVPPQGGRKHPNQDFVQVDTTNILFVCGGAFDGLEKVIRNRSERGGMGFGAEVKS
                     KDDSKAIGEVLRTVEPEDLIKFGLIPELIGRLPVIATLEELSIEALVEILIEPKNALV
                     KQYQKLFSMEGVELEIRPPAMTAIARRALERKTGARGLRSILESVLLDTMYELPGMEN
                     VSKVVIDENMISSDAKPILIYADQPKVSGSN"
     gene            complement(2118..2756)
                     /gene="clpP"
                     /locus_tag="PLD53_09760"
     CDS             complement(2118..2756)
                     /gene="clpP"
                     /locus_tag="PLD53_09760"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRC61243.1"
                     /translation="MSQDRFAAGPDWDPQALGMVPMVIEQSGRGERAYDIYSRLLKER
                     VIFLVGPVNDITANLVVAQLLFLEAENPDKDVHFYINSPGGSVSAGLSVYDTIQFIKP
                     DVSTLCMGQACSMGSFLLAAGTKGKRFALPNSRVMIHQPMGGFQGQASDIAIHAKEIL
                     SLRAKLNEIMAHHTGQPIERIERDTDRDNFLSAQEAAEYGLIDKVLVSREMM"
     gene            complement(2789..4120)
                     /gene="tig"
                     /locus_tag="PLD53_09765"
     CDS             complement(2789..4120)
                     /gene="tig"
                     /locus_tag="PLD53_09765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRC61244.1"
                     /translation="METNASPTSADAAQQTNALERRLDLAVAIADLDKEIDQRLRRMS
                     KNVKMPGFRPGKVPATIVKKQYGEQAHFEALNEALERAFVEAVKTKQLRVAGQPNIEP
                     KASESTTHVEFSAVFEVYPEFKPGDLKDVEIERPVRAVGAAELDSTLTALRKQRVRFE
                     PVDRAAATGDQVMVDFLGKKDGEPFQGGQAKDYPFVIGEGAMLADFENAVIGLKAGEE
                     KTFDMTFPAEYQAKDLAGQAVAFTISVKEVREPVLPEIDADFAKALGVEDGDVDKMKA
                     EIEGNLKREVKKRLQGKVKDQVMDALLKANPVDVPNALVEMEIQRLMQAARQDMEQRS
                     GGKMKDFPLQREWFADQAKRRVSLGLILSEIVKVHELHAKADQVRAIVDEAAQSYEHP
                     EEVVRWYYAQPQRLTEIEGVAIEDNVVTWALASAKVVDKPVDFDELMGHKT"
     gene            complement(4191..4275)
                     /locus_tag="PLD53_09770"
     tRNA            complement(4191..4275)
                     /locus_tag="PLD53_09770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4239..4241),aa:Leu,seq:tag)
BASE COUNT          867 a         1307 c         1116 g          987 t
ORIGIN      
        1 tactcgcgct ggctcttttc catctggcgc ttgacgcgac cgcggatgcg cttctcgacc
       61 tgcaggatgt cgatttccgt ttccagttgc gagagcagac gttcgatgcg gggccgaatt
      121 tcgaacatct cgagcacttc ctgcttctgc tcgatcttca gcggcaaatg cgctgcaatg
      181 gtatcggcca gacggccggc ttcctcgata ctggcaatcg atgtcaggat ttccggtggg
      241 atcttcttgt tgagcttgac atactgatca aattgcgaga tcacggcgcg acgcaggcct
      301 tcaacctcgt gatcaacgcc gtcttcgacg gcgagcggct gcgcgatggc gacaaacatc
      361 tcgtcgcggg cttcaatcga gtgaacttgt gcacgctgcg ttccttcgac cagcaccttc
      421 acggtaccgt ccggcaattt gagcatctgc agtatgttgg caacgcagcc gacactatag
      481 agatcctcgg cctcaggctc atccttgacc gccgatttct gcgccaccag caggatcgac
      541 ttgccggctt ccatcgccat ttcaagcgcc ttgatggatt ttggccgccc cacgaaaagt
      601 ggaatcacca tgtgcggaaa caccaccacg tcgcgtagcg gcaatagtgg cagttcaacg
      661 gactcggcgg atagtgggag attcgtagac atcaaatatt ctcttgtgga gtaggttcat
      721 tgcccgaggt gggggcatga gaccatattg caagccgcgg gtcgcggcaa cacagtcagt
      781 tcgagccgga aaccttcggc tgatctgcgt agatcagaat cggtttggcg tcactgctga
      841 tcatgttttc gtcaatgacg acttttgaga cattctccat acccggaagt tcgtacatgg
      901 tatcgagcag gacggattcg agtatcgaac gcaggccgcg cgcgccagtc ttgcgttcca
      961 gggcacgtcg ggcaatggcc gtcatagccg gtggacgaat ctcgagttcg acgccttcca
     1021 tcgagaacaa tttctggtac tgcttgacca aggcattttt cggttcgatc agtatttcca
     1081 ccagcgcctc gatcgacagt tcctcaagcg tggcaatcac cggcagacgg ccgatcagct
     1141 ctggaatcag accgaacttg atcagatcct caggctcgac ggtacgcaac acctcgccga
     1201 tcgccttact gtcgtccttg ctcttcactt ctgcgccaaa gcccatccca ccgcgctcgg
     1261 agcgattgcg aatgactttc tcgagaccgt cgaaggcacc accgcagacg aaaagtatgt
     1321 tggtggtatc gacctgcacg aaatcctgat tcgggtgctt gcgcccgccc tgtggcggca
     1381 ccgaagccac cgtgccttcg atcagtttga gcaaagcctg ctgtacgcct tcaccggaaa
     1441 cgtcccgggt tatcgacggg ttgtccgatt tgcgtgaaat cttgtctatc tcgtcgatat
     1501 agacaattcc ctgctgtgcc ttgtcggtat cgtaatcgca cttctgcagc aatttctgga
     1561 tgatgttctc gacgtcttca cccacataac cggcttcggt cagcgtcgtc gcatcggcca
     1621 ttacgaacgg cacattgagc atgcgcgcca aggtttgcgc aagaagggtc tttccggaac
     1681 cggtcggccc gatgagcaag atattgctct tggccagctc aatgtcgtca ttgaccttgc
     1741 cgcgatgacg caggcgcttg tagtggttat aaacggccac tgacaagata cgcttggccg
     1801 aatcctgccc gatcacgtac tgatccagca gtccggaaat ctccctcggc gtcggcaggt
     1861 cgctcttcgc gaccttatca cccaggtcag cggagatctc gtcgcgaaca atatcgttgc
     1921 acagctcgat acactcgtcg cagatgaata ccgacggccc ggcgatgagt ttctttacct
     1981 catgctggct cttcccacaa aacgaacagt agagcaattt ttcgctgccg cttttctttt
     2041 ccgtcatctc ggcctacctc tttgtgtatg gtgagaatgc tgcaccccgg caagcgaacc
     2101 cgcagtctcg ccctcggtca catcatttcg cggctaacca gcaccttgtc gatcagacca
     2161 tattctgccg cttcctgagc agaaaggaaa ttgtcccgat ccgtatcgcg ctcgatgcgc
     2221 tcgatcggct ggcccgtgtg atgcgccatg atctcattga gcttggcgcg caaggacagg
     2281 atttccttgg cgtgaatggc aatatccgag gcctggccct gaaatccccc catcggttgg
     2341 tgaatcatca cgcgcgaatt cggaagcgca aaacgcttgc ccttggttcc ggccgccagg
     2401 agaaacgacc ccatggaaca ggcctgcccc atgcacagcg tcgatacatc cggcttgatg
     2461 aactgaatcg tatcgtaaac cgacaaaccg gccgacaccg acccgcccgg cgagttgatg
     2521 taaaagtgga cgtccttgtc gggattctcg gcctcgagaa agagcaactg cgccacgacc
     2581 agattggccg tgatgtcgtt gaccgggccc accagaaaga tcacgcgctc cttgagcagg
     2641 cgcgagtaga tatcgtacgc ccgctcaccc cggccgctct gttcgatcac catcggcacc
     2701 atgcccaacg cctgtggatc ccagtctggg ccggctgcaa aacgatcttg actcatgcca
     2761 atttctccat tgccgaattt gatagcattc acgtcttgtg gcccatcaac tcgtcaaagt
     2821 caacgggttt gtcaacgacc ttggcgctgg cgagcgccca ggtgacgacg ttatcctcga
     2881 tcgccacgcc ctcgatttca gtcaaacgct gtggctgagc atagtaccag cggacaactt
     2941 cttcaggatg ctcgtagctc tgcgctgcct cgtcaacaat cgccctgacc tgatccgcct
     3001 tggcgtgcaa ttcatgaacc ttgacgatct ccgaaagaat caaaccaagg ctgacacggc
     3061 gcttggcctg atcggcgaac cactcgcgct gcaaggggaa atccttcatt ttcccccctg
     3121 agcgttgctc catatcctgg cgggcggcct gcatcaaacg ctgaatctcc atttccacca
     3181 gggcgttggg aacgtcgacc gggtttgcct tgagcaaggc atccatgacc tgatccttga
     3241 ctttgccctg caagcgcttc ttcacctcgc gcttcaaatt gccttcgatc tcggccttca
     3301 tcttgtctac atcgccgtct tcgacaccaa gcgccttggc aaagtcggca tcgatctccg
     3361 gcagaaccgg ctcgcggact tccttgaccg agatcgtaaa cgctaccgct tgccccgcca
     3421 gatccttggc ttgatactcg gcaggaaaag tcatgtcaaa ggtcttttct tcgccggcct
     3481 tgaggccgat caccgcgttc tcaaagtcag ccagcatcgc gccttcgcca atgacaaaag
     3541 gatagtcctt ggcctgaccg ccctgaaatg gctcgccgtc ctttttcccg aggaaatcaa
     3601 ccatcacctg atcacccgtg gccgcggctc gatcaaccgg ctcgaaacgt acacgctgct
     3661 tgcgcagggc cgtcagcgtg ctatccagtt cggcggctcc gactgcacgt accggacgtt
     3721 ctatttcaac gtccttcaga tcgcccggct tgaactcggg atatacctcg aaaactgcgg
     3781 aaaactcgac atgcgtcgtg ctttcgctgg ccttcggttc gatgttcggc tgaccggcaa
     3841 cacgtagctg cttggttttg accgcctcaa caaaggcacg ttcgagcgct tcattcaacg
     3901 cttcaaagtg agcctgctca ccgtactgtt tcttgacgat tgtggccggc accttgcctg
     3961 ggcgaaagcc gggcattttc acgttcttgc tcatccgcct cagtcgctga tcaatctcct
     4021 tgtcgagatc ggcgatagcc acagcaaggt ccagacggcg ctccagggcg ttggtctgct
     4081 gcgcggcgtc ggcgctggtg ggactggcgt tcgtttccat tcaaattcct cgaagtcggg
     4141 tcatatcgtg caggattaat cggacgaaaa tctgcggttg agttgagtcg tggtgcgaga
     4201 gaagggagtc gaacccccac gccttgcggc gctggaacct aaatccagtg cgtctaccaa
     4261 ttccgccact ctcgcgc
//
LOCUS       DAORPM010000214         4265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6359_length_4265_cov_8.951544, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000214 DAORPM010000000
VERSION     DAORPM010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6359_length_4265_cov_8.951544"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLD53_09775"
     CDS             <1..569
                     /locus_tag="PLD53_09775"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRC61245.1"
                     /translation="VPLILSRRPWPATLQAAAIGQGTVAQLAACGIGRVVAPTERYDS
                     EALLDLPVFQAERVAGKKLLILRGNGGRELLADTLRERGAEVEAVTCYHRSAPVDGAL
                     VVSLLRDERLDALTISSSEGLRNLLALLDTDGRKHLCTLPVFVPHQRIAAMAAELGLK
                     NFVLTGPADAGIIKSLNEFDWLHHERHC"
     gene            553..1671
                     /locus_tag="PLD53_09780"
     CDS             553..1671
                     /locus_tag="PLD53_09780"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRC61246.1"
                     /translation="MNDTVDQHSTPAQDSSPPIAEAVASVAPIVPERRKSSYAAWKSP
                     WLYVALVALGLAAWQWYETRTRLADTQQELARRLSENDTVALESRALSKQAQEQFALL
                     QGKLGALEGKLADSQSQQAALEELYQEVARSRDDWALAEVEQSVTLAAQQLQLAGNLQ
                     GAVLALQTAEARIAGSSRPQFISLRKVLARDLERLRALPQVDLPEMSLRLESLIAAVD
                     TMPLAVDGRPQNSFKPKDAFVEASWESLGTQAFWRQLAMELWGEFRSLVRIQRFDRDE
                     PALLAPGQDFFLRENLKLRLLNARLALLAHEQSVFRNELKQTQLWVDRYFDASDKSVQ
                     IARESLKQLSSTEISIAMPNLNESLSAIKNFKRGEERK"
     gene            1668..2852
                     /locus_tag="PLD53_09785"
     CDS             1668..2852
                     /locus_tag="PLD53_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HRC61247.1"
                     /translation="MKGLLWVLTLFALAVGISLVADFNEGYVLLVYPPYRAEISLNLA
                     ILLTLACFVVFYTLLRTLALTLSMPRRVREFRERRRREKAAREFYDVARLIFEGRYSQ
                     ALKKATGAHAAGQSPALAALLAAHSAQRLREPDKQKEWLDRAAQDDPKMQSACLMLEA
                     EMHIEMRCFDEAVAVLKRLQDTSGRHIAALRLEMRAHQGSGNWDEVLRIARLLEKRNA
                     LLPEAAEEIKLKAHRENIRLRQSDLPQIQAYYKKLPSREVSSRLALTYAEALMELGAE
                     DEAQRFIEKQLDKEWNPRLVDIYGLSQGGDPASRIARADKWLPQHHDDSRLLLALGRM
                     CLAQRLWGKAQSYLEASLSLEDRREVRLELARLLEETDRADEATPHYRAAAGNLARLE
                     SK"
     gene            complement(2932..>4265)
                     /locus_tag="PLD53_09790"
     CDS             complement(2932..>4265)
                     /locus_tag="PLD53_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HRC61248.1"
                     /translation="VRMLEMAHAAYGRLPWERLFEPAIELAEAGFPASPRLHQLLKAD
                     THLREDSQAAAYFYQPDGEPHAVGTLLRNVALAKVLRRIAAEGSRALYEGEIAQAVVD
                     RVHNHPANPGQLSMADMAAYKAKRRSALCTNYAPEGAPQIRSYRICGFPPPSSGAIAI
                     DQILGILNDTEARHLRFDNPLWMHYYTEASRLAFADRARYVADPDFVAAPAGGWQSLI
                     SPQYLAERARLIGPQRMKVAEPGIPTAENTSYSPMPEQVEHGTSHISVVDAWGNALAM
                     TSSIEDAFGARQMVRGFLLNNELTDFSFTPTDSSGNPIANRVEPGKRPRSSMSPTLVF
                     DNDSGALVLSGGSPGGAMIIHYTAKLLYATLNWGLTPQQAANLPNFGALTGPTLLEEQ
                     RFTPEFVEALRSRGHQVKELGLSSGVQAIMKVQDGYTGGADPRREGRVMGE"
BASE COUNT          813 a         1249 c         1323 g          880 t
ORIGIN      
        1 gcgtgccctt gattctttcc cggcggcctt ggccggccac gctgcaggcg gcggcgatcg
       61 gacagggcac ggttgcgcag cttgcagcct gcggaatcgg gcgcgtcgtt gcgccgacag
      121 agcgctacga ttccgaggct ctacttgatc tgccggtgtt tcaggccgaa cgcgttgccg
      181 gcaaaaagct attgatcctg cgcggtaacg gagggcgcga gttgctagcc gataccttgc
      241 gcgaacgcgg agctgaagtc gaagccgtga cctgctatca tcgttctgca cctgtcgacg
      301 gagctttggt cgtgtcgctg ctgcgcgacg agcgccttga tgcactgaca atttcatcca
      361 gcgagggtct gcgtaacctt ttggctttgc tcgatactga cggccgcaag catttgtgca
      421 ccctgccggt gtttgttccg catcagcgga tcgctgcaat ggccgcagaa ctcggtttga
      481 agaactttgt tctaaccggg ccggcggatg ccggtatcat taagagtctg aacgaattcg
      541 attggcttca ccatgaacga cactgttgat caacactcca ctcctgcgca agatagttca
      601 ccgccgatag ctgaagcggt cgcgtcggtt gctccgattg tgccggaacg gcgcaaatcg
      661 tcgtacgctg catggaaaag tccctggctt tatgtcgcac tcgttgcgct gggtctggcc
      721 gcctggcagt ggtacgaaac acggacccgc cttgccgata ctcagcaaga gctggcacgc
      781 cgtctatccg aaaatgatac tgttgccctt gagagtcgtg cgctgtccaa acaggcgcag
      841 gaacagtttg cacttttgca gggaaaactc ggcgcgcttg aaggcaagct cgccgactcg
      901 caaagccagc aggcggcact ggaagagctt tatcaggaag tggcaagaag ccgcgacgat
      961 tgggcactgg ccgaggtgga gcagagcgtg acgctggccg cacagcagtt gcagctcgcc
     1021 ggaaatctgc agggggcggt gcttgccttg cagaccgccg aagcgcgtat cgccgggagc
     1081 agccgcccgc agttcatcag cttgcgcaag gtgctggcgc gtgatttgga gcgcctgcgt
     1141 gcgctaccgc aggtggacct tccagaaatg agcttgcgac ttgagagcct gatcgcagcc
     1201 gttgatacga tgccgctggc cgttgacgga cgcccgcaaa atagtttcaa gccgaaggat
     1261 gcctttgtcg aagcgtcttg ggaatcattg ggtactcagg cattctggcg gcaattggct
     1321 atggagctat ggggtgaatt tcgcagtctt gtgcggattc agcgcttcga tcgcgacgag
     1381 ccggccctgc tggcgccggg acaggacttt ttcttacgcg agaatctcaa actgcgtttg
     1441 ctcaatgccc gtctggcgtt gctggcgcac gagcaatcgg tttttcgcaa cgaactcaag
     1501 cagacccagt tgtgggtaga ccgctatttt gacgcgagtg acaaatcggt acagatcgcg
     1561 cgtgaatcgc tcaagcagct atcgtcgacc gaaatcagca tcgcaatgcc aaatcttaac
     1621 gagagccttt cagcgatcaa gaatttcaag cgcggcgagg agcgtaaatg aaaggcctgc
     1681 tctgggtact gacgcttttt gcgctggcgg taggcatctc cctggtggcg gatttcaacg
     1741 agggctacgt cctgcttgtg taccccccgt atcgtgccga gatatcgctg aaccttgcca
     1801 ttctgctcac cctggcgtgc tttgtagttt tttatacgtt gttgcgcaca ctggcactga
     1861 cgctctccat gccacggcgt gtccgcgagt ttcgcgagcg ccgccggcgt gagaaggcag
     1921 ccagagagtt ttacgatgtc gcacgtctca tttttgaagg gcgttattcc caggctttga
     1981 agaaagcaac cggcgctcac gccgcaggtc aatcacccgc gctggctgcg ctgctcgcag
     2041 cccattctgc acagcgtctg cgtgaacccg acaagcagaa ggaatggctc gaccgtgccg
     2101 ctcaggatga tccgaagatg caatcggcgt gcctcatgct cgaagccgaa atgcatattg
     2161 agatgcgatg cttcgatgaa gccgtcgccg ttttgaaacg tctgcaggat acttcgggac
     2221 ggcacatcgc cgccctgcgc cttgaaatgc gcgcgcatca aggcagcggc aattgggacg
     2281 aagtgctgcg catcgcacgg ctgctcgaaa agcgcaatgc cctgctgccc gaggcggccg
     2341 aagagatcaa gctcaaggcg catcgggaaa atattcgcct gcgacagtcg gatttgcccc
     2401 agatacaggc ttactacaag aagctgccgt cgcgcgaggt aagctcccgt ttggcgctca
     2461 cctatgctga agcgctgatg gagctcggcg cagaagatga agcacagcgc tttatagaga
     2521 agcagcttga caaggaatgg aatccacgac ttgtggatat ctacgggctg tcccaaggtg
     2581 gcgatccggc ttcgcgcatt gcccgcgcag acaaatggct gccacagcat catgatgatt
     2641 cccggttgct gctggcgctg gggcggatgt gtttggcgca gcgtttgtgg ggcaaggcgc
     2701 aaagttacct cgaagcatcg ctctcgctgg aagatcgtcg tgaagtgcgg cttgaacttg
     2761 cgcggttgct tgaggaaacc gaccgtgccg acgaggcgac gccgcattac cgggccgccg
     2821 ccggaaatct cgcccgattg gaatccaaat gatcgcggtc gcgatcacgc gattgaaatc
     2881 acttcagtct gcaaccaagt accaaccctg actcgttccg cttccgtagc atcattcgcc
     2941 catcacccgg ccttcgcgac gcgggtccgc cccgccggtg tacccatcct ggactttcat
     3001 gatcgcctgt acgccgctgc tcaggcccag ctccttcacc tgatggccgc gggatcgcag
     3061 ggcctcgacg aattccgggg tgaagcgctg ttcttcgagt agcgtcgggc cggtcagtgc
     3121 gccgaagtta ggcagattgg ctgcctgctg cggcgttaaa ccccagttga gcgtcgcgta
     3181 gagcagtttc gccgtgtagt ggatgatcat cgcgccaccc ggcgagccgc cgctcagcac
     3241 cagcgcacca ctgtcgttgt cgaagaccag cgtcgggctc atcgacgagc gcgggcgttt
     3301 gcccggctcg actcggttgg caatcgggtt gccgctgctg tcggtcggag tgaaggagaa
     3361 atcggtgagt tcgttgttga gcaggaagcc gcgcaccatc tggcgcgcgc cgaaggcatc
     3421 ctcgatgctg ctggtcatcg ccagcgcgtt gccccaggca tcgacgacgc tgatatgcga
     3481 ggtgccgtgc tcgacctgtt ccggcatcgg cgaatagctc gtattctctg ccgtcggaat
     3541 gcccggttcg gccactttca tgcgctgcgg gccaatcagc cgcgccctct cggccaggta
     3601 ttgcgggctg atcaaggact gccacccgcc agccggtgcg gcgacaaagt cgggatcggc
     3661 cacatagcgt gcccggtcgg cgaaagccag gcgcgacgct tcggtgtagt agtgcatcca
     3721 cagcgggttg tcgaaacgca gatgacgggc ctcggtatcg ttcaggatgc cgaggatctg
     3781 gtcgatggcg atcgcgcccg aactcggcgg cgggaagccg caaatccggt agctgcgtat
     3841 ttgcggcgcg ccctctggcg cataattggt acataaggcg gatcgccgtt ttgccttgta
     3901 ggcagccatg tcggccatgc tcaactggcc cggattggcg ggatgattgt gcacccgatc
     3961 gaccacggcc tgcgcgattt cgccttcgta cagggctcgc gaaccttcag cggcgatcct
     4021 gcgcagcacc tttgccagcg ccacattgcg caacaatgtg ccgaccgcat gcggctcgcc
     4081 atcgggctgg taaaaatacg cagcggcttg tgagtcctca cgcagatggg tgtcggcctt
     4141 gagcagttgg tgcaatcgcg ggctggccgg gaatcccgcc tcggccagtt cgatcgccgg
     4201 ctcgaaaaga cgctcccagg ggagcctgcc atatgccgcg tgcgccattt cgagcatccg
     4261 cacgg
//
LOCUS       DAORPM010000215         4219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6480_length_4219_cov_10.861431, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000215 DAORPM010000000
VERSION     DAORPM010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_4219_cov_10.861431"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="PLD53_09795"
     CDS             <1..1853
                     /locus_tag="PLD53_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRC61249.1"
                     /translation="VADLELLNAPEPDWQTVRLDDLVVIAEFRDTIYPGLVSTGRVQR
                     GGDKPNHTVINGENYHVLKALTYTHRGKVDAIYIDPPYNTGAKDWKYNNDYVEGDDLY
                     RHSKWLAMMERRLVISRELLNPAESVLMVTIDEKEYLRLGLMLDQLFADGTMQMISVV
                     INRKGNSRRGEFARCEEYIYIIRFGDAGVSPWHSDMLHESEERGDANVRWKSLLRLAS
                     TGQTEGRFNLFYPLYFENTTYRFIRAGTPIAEGVSIESVPVPEGESVLWPISKDGSDG
                     RWSLSVGKFNEYNKKGYVKFGGRNGGGRTPYYLMSGQVAAIDKGDLQVAGRDDDGAVV
                     LNKITNRRHPMTIWNMPSHSTSGHGTTLLRDALPGRKFPYPKSLYAVEDVLRFFVGEK
                     PNSIILDFFAGSGTTAHAVMRLNRQDGGRRQCISVTNNEVAADEQKELIGRGLRPGEA
                     EWEARGICDYITKPRVQAAITGQTPDGASINGDYKFTDEFPMADGFEENAEFFTLTYE
                     TPVSVNHNRAFERIAPLLWLRAGSRGHRIATLPAEGWAVVEAYGLLVEIDAATPFIEA
                     VCRAPDLRIAYIVTDDDRRFQAIARRLPFGVEPVRLYESYLTNFSFANGE"
     gene            1857..>4219
                     /locus_tag="PLD53_09800"
     CDS             1857..>4219
                     /locus_tag="PLD53_09800"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRC61250.1"
                     /translation="MKFTLKDYQADAVRDALANLKKARKRWQEEAEKCAFSLSAVTGA
                     GKTVMAAAAFEALFHGDDEYDFDADPGAVVIWFSDDPSLNEQTRFRLMEASDRIRHTD
                     MVVVENTFNQPRFQAGKIYFLNTQKLGKNSLLVRGHDQDELEAKAGGLLPETRPDLRS
                     HTIWDTIRNTIEDPSLTLYLVLDEAHRGMGKPTAASVSAKGTIVQQLINGAQGVPGIP
                     VVWGISATVERFKKAMEGAQQRTTLPDVAVDAAKVQESGLIKDTIILDMPDEVGDFDT
                     VLVRRATDKLKASTEAWAEYAQQQEEAHTVLPLMVLQVPNTPDPDEIGRALDTIYQQW
                     PGLPAGSVAHVLGDHSTQQFGNRQVPHIAPERVQESTWVRVLIAKDAISTGWDCPRAE
                     VMVSFRPASDRTHITQLLGRMVRSPLARRIPGHERLNSVDCLLPKFNAKTVTEVVDAL
                     MKGDENAPPPPGRVLVNPVEMQPNPQAAQAVWDKFESLPSQTRPQRGAKPAKRLTALA
                     HELASDDILSGAGKKAHAAMHDVLDQFQQREQALIKARREAVMTVDGKTLAADLKGKT
                     KTFNQFWEDADMAVIDDAYRRAARIFSPDISRTYVDALAYRAAKPEDDDDFVEALVEA
                     RVSVAALGLVTEAQLVFDAAADKLAKSWLATYGATIKRLSHDRQESYRQVREMSTEPQ
                     DVDLVKPEGRFEPTKVRANDVETDIPSYKKHLLCDGKGDYPAQLNEWERKVVEAEMKR
                     EGFSFWYRNPKQPGQSSLGIAYLSREQYSIVRPDFIFFSTQADGQVVA"
BASE COUNT          958 a         1212 c         1273 g          776 t
ORIGIN      
        1 aggtagcgga tctggagcta ctgaacgcgc cagaaccgga ctggcaaacc gtgaggctcg
       61 atgatctggt ggtcatcgcc gagtttcgcg acaccatcta tccgggcctg gtcagcaccg
      121 gcagggttca acgcggcggc gacaagccaa accacaccgt catcaacggc gagaactatc
      181 acgtactgaa ggcgctgacc tacacacacc gcggcaaggt ggatgccatc tatatcgacc
      241 cgccgtacaa cacaggcgcg aaggattgga agtacaacaa cgactacgta gagggcgatg
      301 atctctatcg gcacagcaaa tggctggcga tgatggagcg tcgacttgtg atctcacgag
      361 aactgctgaa tccagcggaa tcggttttaa tggttacaat cgacgagaag gagtatctgc
      421 ggcttggtct aatgttagat caattattcg ctgatggcac catgcaaatg atttccgttg
      481 tgatcaacag gaaaggaaat tcgcggcgtg gcgagtttgc tcgctgcgaa gaatacatat
      541 atatcatccg atttggagat gccggcgtct ctccttggca ttccgacatg cttcacgaat
      601 ctgaggaacg tggcgatgca aatgtccgat ggaaaagtct ccttagactg gcctccacag
      661 gtcaaaccga aggacgtttc aaccttttct acccattgta ctttgaaaac accacatatc
      721 gatttatacg tgccgggaca ccaattgccg agggggtgag cattgagtca gttcccgtgc
      781 ctgagggtga atctgtccta tggcctatct ccaaggatgg tagcgatgga cgatggtctc
      841 tcagcgtcgg taaattcaat gaatataaca agaaaggata tgtcaagttt gggggtcgga
      901 acggcggcgg tcgcactccg tattacctaa tgtcgggtca agtggcggca atcgacaaag
      961 gtgatctgca agtggctgga cgcgacgatg acggcgcggt ggtgctcaat aagatcacca
     1021 atcgtcgtca cccgatgaca atttggaaca tgccttcgca ttcgacatcc gggcatggca
     1081 cgacgttgct ccgtgacgca ctaccggggc gtaaatttcc gtaccctaag tcactctacg
     1141 ccgtcgaaga tgtattgcga tttttcgtag gggaaaagcc aaacagcata attttggatt
     1201 tcttcgcggg gtcgggaact acagcacacg cggtaatgcg ccttaaccgg caagatggcg
     1261 ggagacgtca gtgtatttcc gtcaccaata acgaagttgc tgcagatgaa caaaaagaac
     1321 taatcgggcg agggctgcgc cctggcgagg cggagtggga ggcgcgcgga atctgcgatt
     1381 acataactaa acctcgcgta caggccgcaa tcaccggcca aacacccgac ggtgcatcta
     1441 tcaacggcga ctacaagttc actgatgaat ttccgatggc cgatggcttc gaggaaaacg
     1501 ccgagttctt cacgctgacc tacgaaaccc cggtatcggt caaccacaac cgcgccttcg
     1561 agcgtattgc gccgctgttg tggctgcgcg cgggaagtcg cggacaccgc attgccaccc
     1621 tgcccgccga aggctgggcg gtggtggagg cctatgggtt gctggtggag atcgatgccg
     1681 ccacgccgtt cattgaggcc gtgtgtcgag caccagatct gcgtattgcc tacatcgtca
     1741 ccgacgacga ccgtcgcttt caggccattg ccaggcgctt gccgtttggc gttgagccag
     1801 tgcggctgta cgagtcgtat ctgaccaact tcagctttgc caatggggaa tgagcgatga
     1861 agttcacgct gaaggactac caggccgacg cggtgcgcga tgcactggcc aacttgaaga
     1921 aggcgcgcaa gcgctggcag gaagaagccg agaaatgtgc cttctcgctc agcgccgtca
     1981 ccggcgccgg caagacggtg atggccgccg ctgccttcga agcgctgttc catggggacg
     2041 acgaatacga cttcgacgcc gaccccggtg cggtggtgat ctggttcagc gacgacccct
     2101 cactgaacga gcaaacccgt ttccgcctga tggaagccag cgaccgtatc cggcacaccg
     2161 atatggtggt ggtggaaaat accttcaacc agccccggtt ccaggccggc aagatctact
     2221 tcctgaacac gcagaagctg ggcaagaaca gcttgctggt ccgtggtcac gaccaggacg
     2281 aactggaagc gaaggccggc ggcctgttgc cggagacacg cccagatctg cgttcacaca
     2341 cgatctggga caccatccgc aacaccatcg aagaccccag cctgaccctg tacctggtgc
     2401 tcgatgaagc gcatcgcggc atgggcaaac ccaccgcagc cagcgtgagc gccaaaggca
     2461 ccatcgtgca gcagctgatc aacggcgcgc agggtgtgcc gggcattccg gtggtgtggg
     2521 gaatctctgc gactgtcgaa cgcttcaaga aggccatgga aggcgcccag cagcgcacca
     2581 cgctgcctga tgtggcggtg gatgcagcca aggtgcagga atctggcttg atcaaggaca
     2641 ccatcatcct ggacatgccc gatgaggtgg gcgacttcga cactgtgctg gtgcgccgcg
     2701 ctaccgataa gctgaaggcc tccaccgagg cctgggccga gtacgcgcag cagcaggaag
     2761 aggcgcacac ggtgctgcct ctgatggtct tgcaggtgcc taacacccca gatcccgacg
     2821 aaatcggccg ggcactggac accatctacc agcaatggcc aggcctgccg gcgggcagcg
     2881 tggctcatgt attgggagac cacagtaccc agcagttcgg caaccgccag gtgccccata
     2941 tcgcaccgga gcgggtgcag gagtcgacct gggtgcgcgt actgatcgcc aaggacgcca
     3001 tcagcaccgg ctgggactgc ccccgtgccg aagtgatggt gtcgttccgg cccgcaagcg
     3061 accgtaccca catcacacag ttgttggggc gcatggttcg ctcgccgttg gcgcgccgta
     3121 ttcccggcca tgaacgcttg aactcggtgg attgcctgtt accgaaattc aacgccaaga
     3181 ccgtgaccga agtggtcgat gccttgatga agggcgacga aaacgcgccg ccgccgccgg
     3241 ggcgagtgct ggtcaatccg gtcgagatgc agcccaatcc acaggccgcg caagcggtct
     3301 gggacaagtt tgaatcactg ccgtcgcaga cgcggccgca gcgcggcgcc aaacccgcga
     3361 aacgcctcac agcgctcgcc cacgagctgg cttcggacga cattctctcg ggtgctggca
     3421 agaaggcgca tgcagccatg cacgacgtcc tggaccagtt ccagcaacgc gaacaagccc
     3481 tgattaaggc acggcgcgaa gcggtgatga cggtcgatgg aaagaccctg gcagcggacc
     3541 tcaagggcaa gaccaagact ttcaaccagt tctgggaaga cgccgacatg gcggtgatcg
     3601 acgatgccta ccggcgtgcg gctcgcatct tcagtccaga catttcgcgc acctatgttg
     3661 atgccctcgc ctatcgcgcg gcgaagcccg aagacgacga tgacttcgtg gaggcgctgg
     3721 ttgaagcgcg ggtttccgtt gccgcgttgg ggctggtgac agaagcccaa ctggtcttcg
     3781 atgccgcggc cgacaagctg gccaagtctt ggctggcgac ctacggggcg acaatcaagc
     3841 gcctttcgca cgaccggcaa gagtcgtatc ggcaagttcg cgagatgagc accgagccgc
     3901 aagatgtcga tctggtgaaa ccagagggac gcttcgagcc gaccaaagtc cgtgcgaatg
     3961 acgtcgagac ggacatcccc agctacaaga agcacctact ctgcgatgga aaaggcgact
     4021 acccggcgca actcaacgag tgggaaagaa aggtggtcga ggctgagatg aagcgcgagg
     4081 gctttagctt ctggtaccgc aatccgaagc aaccggggca gtcatcgctc ggcatcgcgt
     4141 acctctctcg cgagcaatac tcgatcgttc gcccggactt catcttcttc agcacacagg
     4201 ctgacggaca ggtcgtggc
//
LOCUS       DAORPM010000216         4150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6641_length_4150_cov_9.967033, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000216 DAORPM010000000
VERSION     DAORPM010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6641_length_4150_cov_9.967033"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLD53_09805"
     CDS             <1..848
                     /locus_tag="PLD53_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRC61251.1"
                     /translation="LHNVMPLIAEAEHGTYYGLMGQASPTAEQLQELQDACAGDMAMM
                     RHCRQCRADAVGMLGEDRGAEFTTDKIDLLELDETDYATAMVRRKEVRDSIIEQLEQK
                     REEKREQQRESERALPPPLAEQDDSAEELLPSLRKVLMAVAGKNGVVGEHFGHAREFL
                     IYEASPGGVRFISHRKTGLYCNGLESCGEGDEVSASASSAASEAESVLDRTIATLAGC
                     EVVLCSKIGYEPWGKLEASGIQPNGEHAMEPIEDAVLAVYKEMASSGKLAEKLAEAVA
                     PRKVA"
     gene            878..1156
                     /locus_tag="PLD53_09810"
     CDS             878..1156
                     /locus_tag="PLD53_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRC61252.1"
                     /translation="MALKITKSCVNCWACVDVCPTDAIYLDTTHFMIDKRKCTECAGE
                     HADPQCASICPIEMAIVNEMGEFLNPAGSLTGIPLEKLIELGLYKAAA"
     gene            1156..1659
                     /locus_tag="PLD53_09815"
     CDS             1156..1659
                     /locus_tag="PLD53_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61253.1"
                     /translation="MATVTFYEKPGCSGNLRQKLLLAASGHTVQARDLNAEAWSADRL
                     LAFLGNLEIGEWFNRSAPLVKSGEIVPDNLGFEQALHLLLDNPLLIRRPLMEIVRGTT
                     TERCVGFDPVVVDAWIGLNHVGFSAGNLESCARDSNRHVSGTGPAHAASDGDGAEKAN
                     HGRCGAH"
     gene            1637..2908
                     /locus_tag="PLD53_09820"
     CDS             1637..2908
                     /locus_tag="PLD53_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRC61254.1"
                     /translation="MAAAALTDRAVEIAPGVHWVGALDPHLRSFDIILKTANGTSYNA
                     YVVRGDSGVAVIDTVKEAFSDDFFRRLESIADYAEITTIVLNHLEPDHSGALPELLRR
                     APQARVYLSSRAQMMLKALLKPSFKEPPEYTPVTTDDTVELGGRTLRFLHTPYLHWPD
                     TQCTYLVEDGILFTGDVFGCHFCDARLFNDSVGDFRFSFEYYYTHIMRPFREFVLDAL
                     ALIEPLDLMLIAPAHGPILRHLPREYLQRYRELARPRLCNEASTEKTLIVFYLSAYGN
                     TRQMAESLAAGAEGIPGVRVSLYDLEGGDAEIFTDLIEEADGLAFGSPTINGDAVKPI
                     WDLLSSLTSVSIKGKLGAAFGSYGWSGEGVRLIEDRLRGLKLRVPIEGLRIKLVPDAS
                     ELEQCRNLGENLARHLTGQFEHRELDLAALA"
     gene            2952..3239
                     /locus_tag="PLD53_09825"
     CDS             2952..3239
                     /locus_tag="PLD53_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRC61255.1"
                     /translation="MPKAKVTFDDVGVSVTVPAGTRLIEVSEKVGSGIIYGCREGECC
                     TCLTKIVSGAENLATPSILEDQVLKDNMAPRKHRLACQAQILGGEVVVRPA"
     gene            3304..3666
                     /locus_tag="PLD53_09830"
     CDS             3304..3666
                     /locus_tag="PLD53_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRC61256.1"
                     /translation="MALITFSSHLHKDKTVYAIAGSHTQTILALAKENHIPIDFGCQE
                     GDCGTCLVKVSSVDGKRRPMGGPLNVREVAALTALGHITKAEVEQMYVDDIPPTQWRL
                     ACQMIVRDEDILVEYPSK"
     gene            3674..>4150
                     /locus_tag="PLD53_09835"
     CDS             3674..>4150
                     /locus_tag="PLD53_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HRC61257.1"
                     /translation="MNGPDRLFYRSWLLAELLLRHAAEAAAADPNRMLLASLIAGQGA
                     GKGCLPAHFGLGTARAAQMLAEYFPGDAPCATERDALEIPEFEDLQKLLLDHRAGERE
                     SERWIADIVATACAGSDHLWQDLGLGSRDELTCLMWVNFPKLARDNVDDMKWKKFLY"
BASE COUNT          863 a         1311 c         1235 g          741 t
ORIGIN      
        1 tcctgcacaa tgtcatgccg ctgatcgccg aagccgagca cggaacgtac tacggtctga
       61 tggggcaagc cagcccgacc gccgagcagc tccaggaact gcaggatgcc tgcgccggcg
      121 acatggcgat gatgcgccat tgccgtcagt gtcgcgccga tgcggtcggc atgctcggtg
      181 aagatcgcgg cgccgaattc accacggaca agatcgatct gcttgagctt gacgaaaccg
      241 actatgccac ggccatggtt cgacgcaagg aagtgcgcga ctcgatcatc gagcagttgg
      301 agcagaagcg ggaagaaaag cgggaacagc agcgcgaatc cgaacgtgcc ctgccgccac
      361 cgctggcgga gcaagacgac agcgccgaag aactcctgcc aagcctgcgc aaggtattga
      421 tggcggttgc cggcaagaac ggcgtcgttg gcgaacactt cgggcacgcg cgcgaattcc
      481 tcatctacga agcctcacca ggcggcgtgc gcttcatcag ccaccgcaag accggccttt
      541 actgcaatgg ccttgaatcc tgcggcgagg gcgacgaggt ttcggcatca gcgtcaagtg
      601 ctgcttccga agcggaatcg gtgctcgaca gaaccatcgc cacactggcg ggctgcgaag
      661 tcgtgctctg ctcaaagatc ggttacgagc cctggggcaa gctcgaggca agcggcattc
      721 aaccgaacgg cgaacacgcg atggagccga tcgaggacgc cgtgctggcc gtgtacaagg
      781 agatggccag ttcaggaaag ctcgctgaaa aactcgcaga agcggttgct cccagaaagg
      841 tggcgtgaaa tgaaatcgat ccatcgggag tcgtgacatg gcactgaaaa tcaccaaatc
      901 ctgcgtcaat tgctgggctt gcgtcgatgt ctgcccgacc gacgcgatct acctggatac
      961 aacgcacttc atgatcgaca agcgcaaatg cacggaatgc gcgggcgaac atgccgatcc
     1021 tcagtgtgcc tcgatctgcc cgatcgagat ggccatcgtc aacgagatgg gcgaattcct
     1081 caatccggcc ggctcgctga ccggaatccc gctcgaaaaa ctgatcgaac tgggcctcta
     1141 taaggcggcc gcgtgatggc tactgtgacc ttctacgaaa aaccaggctg ttcgggcaat
     1201 cttcgccaga agctgctgct tgccgcttcc ggccacaccg ttcaagcaag agacctgaac
     1261 gccgaagcct ggtccgcaga ccgtctgctg gcatttctcg ggaacctgga gatcggcgaa
     1321 tggttcaacc gcagcgctcc gctcgtcaag tcgggcgaga tcgttccgga taatcttggt
     1381 ttcgaacagg cactgcatct gctgctcgac aatccgctat tgatccgccg cccgctgatg
     1441 gagatcgtca ggggaacgac gactgaacgc tgcgtcggct ttgacccggt ggtcgtcgac
     1501 gcgtggatcg gactgaacca cgtcggcttt tcggccggca atctggaatc ctgcgcgcgg
     1561 gattcaaatc gtcacgtttc cggcaccggc cccgcccacg ctgcatccga cggcgacggc
     1621 gcagagaaag ccaatcatgg ccgctgcggc gctcactgac cgcgctgttg aaatcgcgcc
     1681 gggcgtccac tgggtgggtg ccctcgaccc gcatctgcgc agctttgaca tcattctgaa
     1741 gacggccaac ggcaccagtt acaacgcgta cgtcgtgcgc ggcgacagcg gcgtggcggt
     1801 aatcgacacg gtcaaggaag cattctccga cgacttcttc cggcgtctgg agtcaatcgc
     1861 cgactacgcc gaaatcacga ccatcgtcct caatcatctg gagccggacc atagcggtgc
     1921 gctgcccgaa ttgctgcgcc gcgcgccgca ggcccgcgtc tacctgtcca gccgagcgca
     1981 gatgatgctc aaggcgctac tcaagccgag tttcaaggaa ccgccggaat acacgccggt
     2041 caccaccgac gacaccgtcg aactcggcgg ccgcacgctg cgttttctgc acacgcccta
     2101 cctgcactgg ccggacacgc aatgcaccta tctggtggag gacggcatcc tgtttaccgg
     2161 tgacgtcttc ggttgtcatt tctgcgacgc gcgactgttc aacgacagcg tcggcgattt
     2221 ccgcttttcg ttcgagtatt actacacgca tatcatgcgg cctttccgcg agtttgtgct
     2281 cgacgctctg gcgctgatcg aaccgctcga cctgatgctg atcgctccgg cccacggccc
     2341 catcctgcgt cacctgccgc gcgagtatct gcagcgctac cgcgaactgg ccaggccgcg
     2401 actgtgcaac gaggccagca cggagaagac gctgatcgtc ttctacctct cggcctacgg
     2461 caacacgcgg caaatggccg aatcgctcgc cgccggcgcg gaaggcattc ccggcgttcg
     2521 cgtttcgctt tacgacctcg aaggcggcga tgccgaaatc tttaccgacc tcatcgagga
     2581 agccgacggc ctcgctttcg gcagcccgac gatcaacggc gacgcggtca agccgatctg
     2641 ggatctgctc tcgtcgctaa ccagcgtcag catcaaaggc aagctgggcg ccgctttcgg
     2701 ttcatacgga tggagcggag aaggcgtgcg gctgatcgag gaccgcctgc gcggcctcaa
     2761 gctgcgggtg ccgatcgaag gcctgcgcat caagctggtt cccgacgcca gcgaactcga
     2821 acaatgccgc aatctgggtg aaaacctggc tcgacatttg accgggcagt tcgaacaccg
     2881 ggaactcgac ctggcggcgc tcgcctgaat tgagcacgcc agacattcca gccaccacac
     2941 agggagaaac aatgccaaaa gccaaagtca ccttcgacga tgtcggcgtc tcggttaccg
     3001 ttccagccgg cacgcgcctg atcgaagtct cggaaaaagt cggctccgga atcatctatg
     3061 gttgccgcga aggcgaatgc tgcacctgcc tgaccaagat cgtctccggc gcggaaaacc
     3121 tcgccacgcc gagcattctg gaagaccagg tattgaaaga caacatggcc ccgcgcaagc
     3181 accggctggc ctgccaggcc cagatactcg gcggcgaagt cgtcgtccgc ccggcctgaa
     3241 gacaccggaa cggcatggac cgatagatca atttccacca acccctaatc aggagataca
     3301 caaatggccc tcatcacttt cagcagtcac ttgcacaagg acaaaacggt ttatgccatt
     3361 gccggcagcc atacccagac catcctggcg ctggccaagg agaaccacat cccgatcgat
     3421 ttcggttgtc aggagggcga ctgcggcacc tgtctggtca aggtttcatc ggtcgacggc
     3481 aagcgtcgac cgatgggcgg cccgctgaac gttcgcgagg tagcggcgct tactgcgctg
     3541 ggccacatca ccaaggccga ggtcgagcag atgtatgttg atgacatccc gccgacgcag
     3601 tggcgcctgg cttgccagat gatcgttcgc gacgaagaca ttctcgtcga atatccaagc
     3661 aagtgacgct gccatgaacg gcccggaccg cctcttctac cgtagctggc tgcttgccga
     3721 actgctgctc agacatgcgg cggaagcggc ggcggccgat ccgaaccgga tgttgctggc
     3781 cagcctgatc gccgggcaag gtgccggcaa aggctgcctg cccgctcatt tcggcctcgg
     3841 cacggcgcgc gccgcgcaga tgctggccga gtattttccc ggtgatgcgc cctgcgccac
     3901 cgaacgggac gcgctggaaa ttcccgaatt cgaggatctg cagaagctgc tcctggacca
     3961 tcgtgccggc gaacgtgagt ccgagcgctg gatcgccgat atcgttgcca ccgcctgtgc
     4021 cgggtccgac catctctggc aggatctcgg tctgggcagc cgcgacgagc tgacctgtct
     4081 gatgtgggtc aacttcccga aactggctcg cgacaatgtc gacgacatga agtggaagaa
     4141 gtttctttac
//
LOCUS       DAORPM010000217         4119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6727_length_4119_cov_14.201280, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000217 DAORPM010000000
VERSION     DAORPM010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6727_length_4119_cov_14.201280"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLD53_09840"
     CDS             <1..531
                     /locus_tag="PLD53_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRC61258.1"
                     /translation="GEIRDEDTADMALRAAMTGHQVYSTLHTNSAIGAIPRLLDIGIL
                     PDIMAGNIIGIIAQRLVRRLCLHCKTPYQAEAHEARLLGSAVDTPRPVIFRPSGCERC
                     DFQGYRGRLAIMELLRMNADMDELVARRSTFREFRQLADRQGFVPLADDGLRRVLDGS
                     TSIEEVARIVDLTDRM"
     gene            542..1744
                     /locus_tag="PLD53_09845"
     CDS             542..1744
                     /locus_tag="PLD53_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRC61259.1"
                     /translation="MPLYTYKAVSPDGRSVHGRIDALNVIDLEMRLRRMELDLITGDT
                     VSNQSMFGSSGVPRREIIHFCFHLELLVRAGVPILEGLSDLRDTLENPRFREVVASLI
                     ESIEGGQTLSQAMEGQPKVFEKVFVSLIRAGEASGQLPEVLQSLTESLKWEDELASQT
                     KKLIMYPAFVGTVVISATFFLMIYMVPQLKQFVKNMGQDLPVQTQVLFFVSDLLVSYW
                     YVVVLLPILLFLGLKILLHVNPLARVRFDGAKLQFPLVGSILRKIILSRFANTFALLY
                     ASGIPILESIRTTQDVVGNLVVRQGLERVEQLIGEGQNVTAAFHSVGFFPPLVIRMLR
                     VGESTGALDTALINVSYFYNRDVKESVEKVQQLIEPMLTVVLGCLLGWIMLSVLGPVY
                     DVISKIKT"
     gene            1771..3318
                     /locus_tag="PLD53_09850"
     CDS             1771..3318
                     /locus_tag="PLD53_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61260.1"
                     /translation="MPMKTSRLLYLSTQNMSAYSWHSGELTCEGLFASTESGHKEFTA
                     YLKQNPKSFFSILANVSEEGFHLETIPFLQGADRKAIITRKLGQLFFNAELTASLSLG
                     YEKTKRKDERIMLAALTSNEFFSPWLKAIASAGVALSGIYSLPLLAPSLLRKLRLPED
                     KCLLLTVQDQSIRQSYLDKGELYFSRLTPLHNSSISGIAQAFAAEALKLQQYLTSQRL
                     IGRNQPITAHILAHSGALKAVQNSCINTATVHYNIIKVEDCARITGLKTPLPDAHCEQ
                     LFLNLLTVSPPRIQFANDDLLHDHHLGKIRSILHGLGAFAVLACLFYSAQLLYDVYSV
                     TQESQTLRNEAGQARQRYNSIVSTFPPIPTDNATLRRIIDRYVALKKLSSSPDSLYYE
                     ISRAMEAVPEAELDSIDWEVGNSESRAIELTKQTAVANAVPNDSEAAVVRGTLRFASS
                     ANARQILGAFNRLVEALKANPKLHVDALQQPFDIESGKLLKGADTAHEDNKPRSFSLR
                     ITKRLES"
     gene            3315..3935
                     /locus_tag="PLD53_09855"
     CDS             3315..3935
                     /locus_tag="PLD53_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61261.1"
                     /translation="MKLGTADFPKLRFSLLLAALMIGIGAGVVYVSRDWAKSAKLRLT
                     AAQSERNEIVGKLQRVRSEENEIKQKSAMFNELRTRGVIGDEQRLEWVELLKDVRDQR
                     KLIDLKYEIAPQRLLDANPVNGYNFFSSEMKLQLKLLHEEDLTRLLSDLRQRAKALIQ
                     VRKCAVTRLPRGGAENGTAAQLQADCQIEWITLHEMESSPSGGTVR"
     gene            3932..>4119
                     /locus_tag="PLD53_09860"
     CDS             3932..>4119
                     /locus_tag="PLD53_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61262.1"
                     /translation="MNVSRLRAAPGPGCAGLLCVFMLAALLGQTAGAEETSGQLGRLF
                     FTPERRQNLDRLRQLNIQ"
BASE COUNT          907 a         1244 c         1104 g          864 t
ORIGIN      
        1 ggcgaaatcc gtgacgagga cactgcagat atggcgctac gcgccgcaat gaccggccat
       61 caggtctatt caacactgca caccaattcg gccatcggcg ccatcccgcg actgctggac
      121 atcggcatcc tgcccgacat catggccggc aatattatcg gcatcatcgc tcagcgcctg
      181 gttcgccgtc tgtgcctgca ctgcaagaca ccgtaccagg cggaggccca tgaagcacgg
      241 ttgctgggca gcgccgtcga cacgccgcgg ccggttattt ttcgcccttc cggctgcgaa
      301 cgctgcgatt tccaggggta ccgcggccgc ctggccatca tggagctgtt gcgcatgaac
      361 gccgacatgg atgaactggt cgcgcgtcgc agcaccttcc gcgagttccg ccaactggct
      421 gatcgtcagg gctttgtacc ccttgcggat gacggcctga ggcgcgttct cgacggttcg
      481 acctcgatcg aggaggtcgc ccgcattgtc gatctgactg atcggatgtg agggctcgct
      541 catgccgctc tacacctaca aggcggtctc tcccgatgga cgttcggtgc atggtcggat
      601 cgatgccctc aacgtcatcg accttgagat gcgcttgcgg cggatggagc tcgatctcat
      661 caccggtgac accgtcagca atcaaagcat gttcggttcg agcggtgtgc cgcggcgcga
      721 gatcatccac ttctgctttc acctcgaact actggtacgc gccggtgttc cgattcttga
      781 gggcttgtcg gacttgcgcg acacccttga gaacccgcgt tttcgcgaag ttgtcgccag
      841 cctgatcgaa tccatcgagg gcggccagac actttcacag gccatggaag gccaacccaa
      901 agtgttcgaa aaggtctttg tcagcctgat ccgcgcgggc gaagcctccg gccaattgcc
      961 cgaagtgctg caaagtctca ccgaatcgct gaaatgggaa gacgagttgg cctcgcaaac
     1021 gaagaaactc atcatgtacc cggcttttgt cggcacggtc gtcatctcgg caaccttctt
     1081 tctgatgatc tacatggtgc cgcagctcaa gcagttcgtt aagaacatgg ggcaggactt
     1141 gccggtgcaa acccaggtgc tgttcttcgt ttccgatctg cttgtttcct actggtatgt
     1201 cgtcgtgctg ttacccattc tcctgtttct cggcctgaag atactgctgc atgtcaatcc
     1261 gctggcccgc gtccgcttcg acggggccaa gctgcagttc cccctcgtcg gcagtattct
     1321 gcgcaaaatc atcctctccc gctttgccaa cactttcgcc ctgctttacg cctccggcat
     1381 accgattctc gaatcgatcc gcaccacgca ggacgtcgtc ggcaatctgg ttgttcggca
     1441 aggactggaa cgtgtcgaac aattgatcgg cgagggccag aatgtcacgg cggcgttcca
     1501 tagcgtcggt ttcttccctc cgctggtgat ccggatgctg cgcgttggcg aaagcacggg
     1561 cgcgcttgat accgcgctga tcaatgtcag ttatttttat aatcgtgacg tcaaggagtc
     1621 ggtcgaaaag gttcagcaat tgatagagcc catgcttacc gtcgtcctcg gctgcctgct
     1681 cggatggatt atgctctcgg tcctcgggcc ggtgtacgat gtcatcagca agataaagac
     1741 ttaattggaa gcggactccg gaaccgggca atgccaatga aaaccagtcg cctgctatat
     1801 ctcagcaccc agaatatgag cgcctacagt tggcattcgg gggagctgac ctgcgagggg
     1861 ctgttcgcgt cgactgaaag cggccacaag gaattcaccg cttacctcaa acagaacccc
     1921 aagagtttct tttcgattct cgccaacgtt tccgaagaag gcttccacct cgagaccatc
     1981 ccctttctgc aaggggcgga ccgcaaggcc atcatcaccc gcaagctcgg acaattgttc
     2041 ttcaatgctg aactgaccgc ctcgctatcg cttggctacg aaaagacaaa gcgcaaagac
     2101 gagcgcatca tgctcgcggc actgaccagc aatgaattct tctccccctg gctaaaagcc
     2161 atagcaagcg ccggcgtcgc cctgtcgggc atttactcgc taccgctgct cgcaccgtcg
     2221 ctgctgcgaa aactgcgcct tcccgaagac aaatgcctgc tgcttacagt acaggatcaa
     2281 agcatccggc aaagctatct tgataaaggc gagttgtatt tcagccgact cactccattg
     2341 cataacagca gcatcagcgg catcgcgcag gcctttgccg ccgaggcgct caagcttcag
     2401 caatacctca ccagccagcg cctgatcggg cgcaaccaac ccattactgc ccacatcttg
     2461 gcgcactccg gagcgctgaa agccgtccag aacagttgta tcaataccgc aacggttcac
     2521 tacaacatta tcaaagtcga ggactgcgcc aggataaccg ggctcaagac accgctcccg
     2581 gacgctcatt gcgagcaact ctttctcaat ctgctgactg tctcgcctcc gcgtatccag
     2641 tttgcaaacg acgatctgtt gcatgaccat catctcggga agattcggtc aatccttcac
     2701 ggattggggg catttgccgt gctcgcctgc ctgttctact ccgctcagct gctatacgat
     2761 gtttattcag ttacgcagga atcccagacg ctcagaaacg aagccggcca ggcacgtcag
     2821 cgctacaaca gtattgtcag cacctttcca cccataccga ccgataacgc aacgctgcgc
     2881 cgaatcatcg atcgttatgt tgcactgaaa aaattgagca gttcccccga ttccctgtac
     2941 tacgaaatca gtcgcgcaat ggaagccgtg ccggaagcgg aactcgacag catcgactgg
     3001 gaagtaggga acagcgaatc ccgtgcaatc gagctcacta aacaaaccgc agtggcaaat
     3061 gccgtgccga acgacagcga agccgcagta gtgcgaggaa ccctgagatt tgcttccagc
     3121 gccaatgccc ggcaaatcct gggggccttc aaccgactcg ttgaggctct caaggccaat
     3181 ccgaaactgc acgtcgatgc acttcaacaa ccatttgaca ttgagtcagg aaagctgctt
     3241 aaaggcgccg acaccgcgca cgaagataac aaaccgcgct ctttcagcct gcggattacg
     3301 aaaaggctcg aatcatgaag ctcggcaccg ctgattttcc gaaattgcgg ttcagtctgc
     3361 tgcttgccgc cctgatgatc ggcatcgggg ccggggtcgt ttatgtctct cgcgactggg
     3421 cgaaatcggc gaagctgcgt ctgacggccg cgcagagcga acgaaacgaa atcgtcggca
     3481 aacttcaacg tgtgcgcagt gaagagaacg agatcaagca gaagtcagca atgttcaacg
     3541 aacttcggac gcgtggtgtt atcggcgatg aacaacggct cgaatgggta gagcttctca
     3601 aggacgtacg cgatcagcgc aagttgatcg acctgaaata cgagatcgct ccacaacgtt
     3661 tgctcgatgc caacccggtc aacggataca atttcttttc cagcgagatg aaactgcaac
     3721 tcaagctcct gcacgaggaa gacctgacgc gtctgctcag cgatctgcgc caacgcgcca
     3781 aagccctgat tcaggtccga aaatgtgccg tcacgcgcct gccacgcggc ggcgcggaaa
     3841 atggaaccgc ggcccaattg caggccgatt gccagatcga atggattacg ctgcacgaaa
     3901 tggaaagcag tccgagcggg ggaacagtcc gatgaatgtt tctcgactcc gggcagcacc
     3961 gggcccagga tgcgcggggc ttctgtgcgt cttcatgctc gctgccttgc ttggtcagac
     4021 ggccggcgcc gaggaaactt ccgggcaact cggccggctg ttcttcacac cggagcggcg
     4081 gcagaatctt gatcgtctgc gccagctcaa tattcagga
//
LOCUS       DAORPM010000218         4116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6736_length_4116_cov_8.157350, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000218 DAORPM010000000
VERSION     DAORPM010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6736_length_4116_cov_8.157350"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLD53_09865"
     CDS             complement(<1..405)
                     /locus_tag="PLD53_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61263.1"
                     /translation="MAYTFTEKRRIRKDFGKRSSILDVPYLLAIQLDSYREYLQAEAP
                     PGERREIGLHAAFRSVFPIISYSGNARLEYVSYRLGDPVFDVRECQLRGLTYAAPLRV
                     RLRLVLMDKEAEAGAHKIKDIKENEVYMGELPL"
     gene            complement(607..978)
                     /gene="rplL"
                     /locus_tag="PLD53_09870"
     CDS             complement(607..978)
                     /gene="rplL"
                     /locus_tag="PLD53_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRC61264.1"
                     /translation="MAVSKEDILETIAGMTVMEIVDLISAMEEKFGVTAAVAMAAAPA
                     AAAAAPVEEKTEFDVVMSSFGEKKVEVIKVVRALTGLGLKEAKDLVEGVPSMIKEAIP
                     KAEAEDIKKKLEEAGAKVDVK"
     gene            complement(1078..1614)
                     /gene="rplJ"
                     /locus_tag="PLD53_09875"
     CDS             complement(1078..1614)
                     /gene="rplJ"
                     /locus_tag="PLD53_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRC61265.1"
                     /translation="MSLNLAEKQAVVAEVAKVAARAHSAIAAEYRGLSVTQITNLRVK
                     ARETGVYLRVVKNTLARRALQGTEFECLRASLVGPLILAFSQEDPGAAARLFKEFLKE
                     KAHDKLIVKALAVGGQTFPASELDRLASLPTRNEAISLLMATLRAPLDKLAQTLNEIP
                     SKLVRTLDAVRQQREEAA"
     gene            complement(1836..2531)
                     /gene="rplA"
                     /locus_tag="PLD53_09880"
     CDS             complement(1836..2531)
                     /gene="rplA"
                     /locus_tag="PLD53_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRC61266.1"
                     /translation="MAKLSKRVQAIRAKLTPGKFYPVEEALSLLKELSGVKFREAVDV
                     AVNLGVDPRKSDQVVRSATVLPHGTGKTVRVAVFAQGANADAALAAGADVVGFEDLAE
                     SVKAGNLDFDVVIASPDAMRVVGQLGKILGPRGLMPNPKVGTVTPDVVGAIRNAKAGQ
                     VRYRTDKAGIIHCTIGKVDFAPNQLRENLQALLHDLQKIKPATSKGVYVRRVTVSTTM
                     GPGLAVDQATLSF"
     gene            complement(2535..2966)
                     /gene="rplK"
                     /locus_tag="PLD53_09885"
     CDS             complement(2535..2966)
                     /gene="rplK"
                     /locus_tag="PLD53_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRC61267.1"
                     /translation="MAKKVTAYVKLQVAAAKANPSPPVGPALGQHGVNIMEFCKTFNA
                     QTQHLEPGLPIPVVITVYSDRSFTFIMKTPPASVLLRKMLGLAKGSANPNTKKIGTVT
                     LAQLEDVAKAKMPDLTAASLDAAVRTIAGSARSMGLNVEGV"
     gene            complement(3056..3595)
                     /gene="nusG"
                     /locus_tag="PLD53_09890"
     CDS             complement(3056..3595)
                     /gene="nusG"
                     /locus_tag="PLD53_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRC61268.1"
                     /translation="MAMRWYVVQAYSGFEQQVKRALLERINREGLIDRFGQVLVPTEE
                     VVEMRDGQKRKSDRKFFPGYVLVQMEMDNETWHLVRNTPKVLGFIGGTSDKPAPISEK
                     EAQTILQRVQEGVDKPRPKVLYEPGEMVRVTEGPFNDFEGVVEEVNYEKSRLRVGVMI
                     FGRTTPVELEFSQVEKVSR"
     gene            complement(3608..>4116)
                     /gene="secE"
                     /locus_tag="PLD53_09895"
     CDS             complement(3608..>4116)
                     /gene="secE"
                     /locus_tag="PLD53_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRC61269.1"
                     /translation="VSKTAGWGFDSLLACHLSDGTPRLRSPVLAARTYVGVRKARVRM
                     NSTKPETQASEHADTYKLLAAGFIILIALVAFYVFANHSLLARVLGLLAAIGVAAWIA
                     MKTELGVETLEFMQGSRAELRKVVWPTRTETTQTTLIVIAMVVIMGLLLWLLDVLLFW
                     LVRLITTG"
BASE COUNT          884 a         1250 c         1092 g          890 t
ORIGIN      
        1 cagcggcagt tcgcccatgt agacttcgtt ttccttgatg tccttgatct tgtgagcgcc
       61 agcttcggcc tccttgtcca tcagcaccag ccgcaaccgc acccgcagcg gcgcggcgta
      121 ggtcagcccc cgcaactggc actcccggac atcgaacacc ggatcgccca gccgatagct
      181 cacatattcc agccgagcgt tgcctgaata gctgataatc gggaacaccg agcggaacgc
      241 ggcatgtaac ccaatctcgc ggcgttctcc cggcggcgcc tcagcttgca ggtattcacg
      301 atacgaatcc aactggatcg ccagcaaata gggcacatcc aggatgctgg atcgcttgcc
      361 gaagtcttta cggatgcgtc tcttttcagt aaaagtatag gccatcggga ttcctctcac
      421 gggagtgcca gccaggggcg ggagccgacc acacccgccc tgcaagatga caggcggggt
      481 ggattaaacc ccctgacggg ttcaaacgga aacaggccgg caacccgggg ccgccagccg
      541 ttccagtccg tccaagctag acaatgacgc gcaatgcaat cccacgggac agtgggcaca
      601 ctacgcttac ttgacgtcga ctttggcgcc agcttcttcc agcttcttct tgatgtcctc
      661 ggcctctgct ttgggaatcg cttccttgat catggaaggc acgccttcca ccagatcctt
      721 ggcttccttc agtcccaaac cggtcagagc gcgcaccacc ttaatcacct cgaccttctt
      781 ctcgccgaag ctgctcatca ccacatcgaa ctcggttttt tcctcaaccg gagccgctgc
      841 ggcagcggct ggagccgcag ccatagccac agcagcagtg acgccaaact tctcttccat
      901 cgcagaaatc aggtccacga tttccatcac ggtcatgcca gcgatggttt ccagaatgtc
      961 ttctttcgaa acggccatta cccatttact cctgataggg acgctatcat tcgaccagtg
     1021 ttaattgaca agtcctgatc aatacatttt gatcattgga ctgtttttcc aagataatca
     1081 ggcagcttct tctctttgct gacgaaccgc atcaagagtg cgcaccagtt tgcttggaat
     1141 ttcgttgagg gtctgcgcga gtttgtcgag cggcgcccgc agagtagcca tcagcaggct
     1201 gatcgcttca ttgcgcgtcg gcaagctggc cagacgatcc agctcgctgg ctggaaaagt
     1261 ctgtccaccg accgctaacg ctttgacgat cagcttatcg tgggcttttt ccttgagaaa
     1321 ctccttgaac aaccgcgctg ccgcgcctgg atcttcctgg gagaacgcta gaatcaacgg
     1381 tcccaccaaa ctggctcgca ggcactcgaa ctcggttccc tgcaacgcgc gccgcgccaa
     1441 ggtgttcttg acaacacgca gatagacgcc ggtttcccgc gcttttacgc gcagattcgt
     1501 gatctgagtg acactcagcc cacggtactc ggcggcaatt gcggagtgcg cacgggcagc
     1561 caccttggcg acttcggcca ctacagcctg tttttccgcc agattgagac tcattagtta
     1621 cctcgtgatt tttcctccac cggggtggag aacggaaacg gcggcttcac caatcattcg
     1681 gacgagcctt accgtctgcg cgggctaccc cgaatgttga ccgggagcga ttaagtcttg
     1741 cgacgcctgc ggtctttgac actgaccgga aactccggtc ggctcaaagt aggttgagca
     1801 tcttctactc ggcgttcgag cggagatgcc agagttcaga acgacagcgt ggcctgatcc
     1861 acagccaggc ctggccccat cgtggtggac acagtgaccc tacggacata cacacccttg
     1921 gatgtagcag gtttgatctt ttgcaaatcg tgcaatagcg cctgtaaatt ttcccgcaac
     1981 tgattggggg caaaatccac cttgccgatg gtgcagtgga taatgcccgc tttgtcggtg
     2041 cggtagcgaa cctgaccggc cttggcgttg cggatcgcgc cgacgacatc cggggtcacg
     2101 gtgcccacct tggggtttgg cattaggccg cgcggaccga ggattttgcc gagttgaccg
     2161 accacccgca ttgcgtcggg tgaagcaatc accacgtcaa aatcaagatt acccgccttg
     2221 accgattcag ccaaatcctc gaacccgacc acatcggcgc cggcagccaa ggcggcatcc
     2281 gcattggcgc cttgagcaaa caccgctacc cgcacggtct tgccggtgcc atgaggcagc
     2341 accgtggcgc tacgcacgac ctggtcggat ttacgggggt cgacacccag attcaccgcc
     2401 acatccactg cttcccgaaa cttgacgccg gacaactcct tcagcagact gagggcttct
     2461 tcgaccggat agaacttgcc gggtgttagc ttggcgcgga tcgcctgcac gcgcttggag
     2521 agtttagcca tagctcaaac accctccacg ttaaggccca tggatcgggc gctacccgcg
     2581 atagtccgca ctgcggcatc gagactggcg gcggtcaaat cgggcatctt agccttagcc
     2641 acgtcttcca gttgggcgag agtcacggtg ccgatcttct tggtgttggg attagcgcta
     2701 cccttggcga gacccagcat cttgcgcagc agcaccgaag cgggcggagt tttcatgatg
     2761 aaagtgaaac tgcggtcgct gtagaccgta atcaccacgg gaatcggcag tccgggctcc
     2821 agatgctggg tctgagcgtt gaaggtcttg cagaattcca tgatattgac gccgtgctga
     2881 cctagcgcag gacccaccgg aggactgggg ttagccttgg cggctgcgac ctgcaacttg
     2941 acgtatgcgg taactttctt tgccattgtt actcctgtcg ggtgcgagcg ccttacggct
     3001 ccccgaatga gccatctgaa taatcggatg gcagaatagc gaccagtgga actggtcagc
     3061 gggatacttt ttcgacctga ctgaattcca gctccacggg agtggtgcga ccgaagatca
     3121 ttacacccac ccgcagccgg cttttctcat agttgacttc ctcaacgaca ccctcgaagt
     3181 cgttaaaggg gccttcagtg acacgcacca tttcgccggg ttcgtagagc accttgggcc
     3241 gaggtttgtc aacgccttcc tgaacccgct gcaaaatagt ctgagcttcc ttttccgaaa
     3301 tcggcgctgg tttgtcgctg gtgccgccga tgaaacccag caccttggga gtattgcgaa
     3361 ccaagtgcca ggtctcgtta tccatttcca tctgcaccag cacatagcca gggaagaatt
     3421 tacggtcact tttgcgcttc tgcccatcgc gcatttccac cacttcttca gtgggtacta
     3481 gaacctgacc gaagcgatct atcaggcctt cacggttgat ccgctccagc aacgcccgct
     3541 taacctgctg ctcgaagccg gaataagcct gtaccacata ccagcgcatc gccatgctgt
     3601 agctccatta gccggtggtg atgagtcgaa ccagccagaa cagcagcaca tccagcaacc
     3661 acagcaacag gcccatgatc accaccatag cgatcacaat caaggtagtc tgagtcgttt
     3721 cggtgcgtgt aggccagacc accttgcgca attcagcacg agatccttgc atgaactcaa
     3781 gagtttctac accgagttca gtcttcatcg cgatccatgc tgctacgcca atcgctgcta
     3841 gcaaacccaa gacgcgcgcc aacagcgaat ggttcgcgaa gacgtagaaa gccactaatg
     3901 caatcaggat gataaaaccg gcagccaaca atttataagt gtcagcgtgc tcggaggctt
     3961 gcgtttcggg tttagttgaa ttcatgcgga ctcttgcttt gcgcactcca acgtatgttc
     4021 gcgccgcaag aacgggcgaa cgcaatcggg gcgtgccatc ggaaagatgg caggccagga
     4081 gggaatcgaa cccccaacct gcggttttgg agaccg
//
LOCUS       DAORPM010000219         4107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6757_length_4107_cov_14.385242, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000219 DAORPM010000000
VERSION     DAORPM010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6757_length_4107_cov_14.385242"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="PLD53_09900"
     CDS             complement(<1..1300)
                     /locus_tag="PLD53_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRC61270.1"
                     /translation="MRLDKLTTKFQQALADAQSLAIGHDNQMIEPQHLLLALLQQDDG
                     GTASLLAQAGVNVQSLKASLDQAVDRLPKVEGHGGEVQIGRDLTNLLNLTDKEAQKRG
                     DQFIASEMFLLAVCDDKGETGRLAKQHGLSRKALELAVASVRGGQGVDNQEAEGQRES
                     LKKYCIDLTERARQGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNDEVPETLKGKRVLSLDMAALLAGAKYRGEFEERLKAVLKEIALDEGRIIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYVEKDAALERRFQ
                     KVLVDEPSVESTIAILRGLQDRYELHHGVAITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIRMEIDSRPEVMDKLDRRMIQLKIEREAVRKEK"
     gene            1520..3163
                     /locus_tag="PLD53_09905"
     CDS             1520..3163
                     /locus_tag="PLD53_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRC61271.1"
                     /translation="MSIDYKGASLRTKLNGLTVITIVGLCILVVIVLMGEKSQLLADR
                     KNKIRNLVEVAQATISGYEKQAKEGKLSVEEAKRLAIDAVRGMRYDKVEYFWINDLKA
                     VIVMHPIKPELDGKDLSEFKDKNGKLIFVEFAKIAKDQGGGFVDYVWPKPGSEAAVPK
                     ISYVMGVEGWGWVVGSGIYVDDVDALFRQNAMKLLAWVAVIGGFIGVSLTLVSRNVLK
                     LMGGDPQVARQITQRIVDGDLSSEIVCVPGDTTSLLYGMKEMQETLRTMIRSILNNAA
                     TLSTASSQLLTTFENVSEHAQQHGESASSMAAAVEEMTVSIDQVAENAQEAHSISMQA
                     SELSTEGTHIIESAASEMQKISDAVQSSATVIEDLGRHSEQISSIVNTIKEIADQTNL
                     LALNAAIEAARAGEQGRGFAVVADEVRKWAERTSLSTTEIASMVGKIQNGTRSAVSSM
                     QSGVVQAGKGVELATQAGNSINEIRSGSMRVTEVVNSISEAIREQVTASSEIAKSVEK
                     VAQMSEENAVAMKDTTQSAHHLKQLSSSLNDSVSRFKLN"
     gene            complement(3187..>4107)
                     /locus_tag="PLD53_09910"
     CDS             complement(3187..>4107)
                     /locus_tag="PLD53_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61272.1"
                     /translation="AGGGMLNAKLLRAQRACCDGGEAMFSEQMMIESDFMAMFREEWR
                     LARGSTPLGCIAIVDESPATQYLQPEFELFRQLFESSGFTAVIVDPGELSFDGQRLTF
                     GGKPVDLVYNRLTDFSLDDERNQALRAAYLADTVVLTPHPRAHALYADKRNLVALTDE
                     AWLQAIGVSEVDCKLLKSGIPLTKEVLPEDGKSFWTNRKKWFFKPTSGYGSKAAYRGE
                     KLTRRVFNEISHGGYVAQALVSPSERRLLVDGVEQDLKLDLRNYVYHGRVQLVSARLY
                     QGQTTNFRTPGGGFAAVFSVPCQGGGRVCT"
BASE COUNT          868 a         1165 c         1197 g          877 t
ORIGIN      
        1 ccttttcctt gcgcacggcc tcgcgctcga tcttgagctg gatcatgcgg cggtcaagtt
       61 tgtccatcac ttcgggacgc gagtcgattt ccatcctgat ccgcgccgcg gcctcgtcga
      121 tcaggtcgat ggccttgtcc ggcaaaaagc ggtcggtgat gtagcgatgg ctcaattcgg
      181 ccgcggcaac gatggccggg tcggtaatcg cgacgccgtg gtgcagttcg tacctgtcct
      241 gcaggccgcg caagatggcg atcgtgcttt cgaccgacgg ttcatcgacg agcaccttct
      301 ggaagcgccg ctccagtgcc gcgtcttttt ccacgtattt gcggtattcg tcgagcgtgg
      361 tcgcgccgac gcaatgcagc tcgccacgcg ccagtgccgg cttgagcatg ttgccggcat
      421 caatggcgcc ttcggccttg ccggcgccga ccatggtgtg caattcgtca atgaagacaa
      481 tgatgcggcc ctcgtcgagt gcgatctcct tgagcacggc tttcagccgc tcctcgaact
      541 cgccgcgata tttcgcgccg gccagcaagg ctgccatgtc cagactgagc acccgcttgc
      601 ctttgagcgt ttccggcact tcgtcattga caatgcgctg tgcaaggcct tcgacgatgg
      661 ccgtcttgcc gacacccggc tcgccgatca gcaccgggtt gttcttggtg cgtcgttgaa
      721 gaatctgaat ggaccggcga atttcatcgt cgcggccgat caccgggtcg agtttgccct
      781 gacgcgcgcg ttcggtgagg tcgatgcagt atttcttcag cgattcgcgc tgtccttcgg
      841 cttcctgatt gtcgacgccc tggccgccgc gtaccgaggc caccgccagc tccagcgcct
      901 tgcgggaaag tccatgctgc ttggcgaggc gaccggtctc acctttgtcg tcgcagacgg
      961 ccagcaagaa catctcgctg gcgatgaact ggtcgccgcg cttctgcgct tccttgtcgg
     1021 taagattaag caggttggtc agatcgcgac cgatctgcac ctcaccacca tggccttcga
     1081 ccttcggcaa gcgatccacc gcctgatcca aagaggcctt gagcgattgc acattgacac
     1141 cggcctgcgc cagcagcgaa gccgttcccc cgtcgtcctg ctgcaggagg gccagcagca
     1201 agtgttgcgg ctcgatcatc tgattgtcgt gaccaatggc caggctttga gcatcggcca
     1261 gagcctgctg gaacttggtg gttagcttgt cgagacgcat gatgaatcct tttggagaag
     1321 atcgaatatc gcgcaggtgg gggaatccga gcggatttca agtgccagcg ctacattgtc
     1381 ttcgtctttc atatgacgca agtttttttc gcaatcagag taattgccaa cgccttcctt
     1441 aaagggtaga ttgagtgcgg ggtgagttgt ctggttgtct agcacccttc acagtgtccg
     1501 gacggtaaaa ggagagaata tgagcatcga ctataagggg gcgtcactgc gtaccaagct
     1561 gaatggactc acggtgatca cgatcgtagg tctttgcata ttggtagtca tcgtgctgat
     1621 gggtgaaaag agtcagttgc tcgccgaccg caaaaacaag atacgaaatc tcgtcgaggt
     1681 cgcgcaagcc acgatcagcg gctacgagaa acaggccaag gagggcaagc tgagtgtcga
     1741 agaagcgaag cggcttgcca tcgacgccgt tcgcggcatg cgttatgaca aagttgagta
     1801 tttctggatc aacgatctca aagccgtcat cgtcatgcat ccgatcaaac cggaactaga
     1861 tggcaaggat ctttccgagt tcaaagacaa gaacggtaaa ctcattttcg tcgaattcgc
     1921 caaaattgcc aaagatcagg gcggtggctt tgttgactac gtctggccga aaccaggcag
     1981 cgaagcggcg gttccaaaga tttcctacgt catgggtgtg gagggctggg gatgggttgt
     2041 aggcagcggc atctacgtcg acgacgttga cgccctgttt cgtcagaatg ccatgaagct
     2101 tctcgcctgg gtcgcggtaa tcggcggctt cattggagtt tctttgacac tggtttcgcg
     2161 caatgtgctc aagctgatgg ggggcgatcc ccaggtcgcg cgacagatca cacaacgcat
     2221 cgtcgatggc gacctgagct cagaaatcgt ttgcgtgcca ggcgatacca ccagccttct
     2281 gtatggcatg aaggaaatgc aggaaacgct gcgcaccatg atcagaagta tcctgaacaa
     2341 tgcggctacg ctttcaaccg cgtcatcgca gctcctgacg acctttgaaa acgtttccga
     2401 gcatgcccag caacatggcg aatcggcttc ctcaatggcc gctgccgtcg aagaaatgac
     2461 agtcagtatc gatcaggtcg ccgaaaacgc acaagaggcg catagcatct cgatgcaggc
     2521 cagcgagctc tccacggagg gaacgcatat catcgaaagt gccgccagcg aaatgcagaa
     2581 gatctccgat gcggtgcaat cgtctgccac ggtcatcgag gatctgggtc gccattccga
     2641 gcagatttcc tcgattgtca ataccatcaa ggagattgcc gaccagacca acctgcttgc
     2701 gctcaatgcc gcgatcgaag cggcacgtgc gggggaacag gggagaggtt ttgccgtggt
     2761 ggccgatgaa gtgcgcaagt gggccgagcg caccagcctg tcgacaaccg aaattgccag
     2821 catggtcggg aaaatccaga atggcacgcg cagcgcagtc agcagcatgc aaagcggggt
     2881 tgtccaagcc ggcaagggcg ttgagctggc tacccaggcc ggcaattcga tcaatgagat
     2941 ccgttcaggt tcgatgcgcg tcaccgaagt ggtcaacagc atttccgaag ccatccggga
     3001 gcaggtgacg gcttccagcg aaatcgccaa gagtgtcgag aaggtggcgc agatgtccga
     3061 agaaaacgct gtggcaatga aggatacgac gcaatccgca catcatttga aacagctttc
     3121 ttcttcgctg aacgattcgg tcagccgctt caagttgaac tgaccgaatc gaagagtctg
     3181 cgcgtcttac gtgcagactc ttcccccacc ctggcacggc actgaaaata ctgctgcgaa
     3241 gcccccgcca ggcgtgcgga agttcgtcgt ctgtccctgg tacagccgag ccgaaacgag
     3301 ttgcactcgc ccgtgataga cgtaattgcg caggtccagc ttgagatcct gctcgacacc
     3361 atcgaccagc agtcgtctct ctgatgggga aaccagtgcc tgggcgacat agccaccgtg
     3421 gcttatctcg ttgaaaacgc gtctggtgag cttttcgccg cgatacgcgg ctttgcttcc
     3481 gtagcccgat gtcggtttga agaaccactt cttgcggttc gtccagaacg acttgccatc
     3541 ctctggcaat acctctttgg tgagcgggat gccggatttc aatagtttgc agtccacttc
     3601 gctgacgcca atcgcctgta accaggcttc atctgtcaag gcgaccagat tgcgcttgtc
     3661 ggcatagagc gcatgggccc gcgggtgagg ggtgagcacc acggtgtcag cgagataggc
     3721 cgcccgcaat gcctggttgc gttcatcatc aagcgaaaag tcggtcagcc ggttgtagac
     3781 cagatcgaca ggctttccgc cgaacgtcaa gcgctggcca tcgaaggaaa gttcgccagg
     3841 gtcgacaatc actgcagtga atccactcga ttcgaataat tgccggaaca actcgaactc
     3901 gggctgcagg tactgtgttg ccggcgactc gtcgacgatg gcgatacagc cgaggggtgt
     3961 gctaccgcgc gccagtcgcc attcctcgcg aaacatggcc atgaaatccg actcgatcat
     4021 catctgctcg gagaacatcg cttctcctcc gtcgcagcag gcgcgttgcg cgcgcagcaa
     4081 tttagcattg agcatgccgc caccggc
//
LOCUS       DAORPM010000220         4086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6814_length_4086_cov_22.448524, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000220 DAORPM010000000
VERSION     DAORPM010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6814_length_4086_cov_22.448524"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1421
                     /gene="carA"
                     /locus_tag="PLD53_09915"
     CDS             273..1421
                     /gene="carA"
                     /locus_tag="PLD53_09915"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRC61273.1"
                     /translation="MPSFIPAILALADGTVFKGQSIGADGVTSGEVVFNTAMSGYQEI
                     LTDPSYCRQIVTLTYPHIGNTGCNAEDFESDANYAAGLVIRDLPPRASNWRMQEDLSS
                     YLKKHGIVAIAGIDTRKLTRILREKGAQAGCILAGEVDESRALALANDFPGLAGMDLA
                     KVVSAKAGYTWSEGEWTLNGYKPGPEPRFHVVAYDFGVKRNILRMLAQRGCKLTVVPA
                     QTPAADVLAMNPDGIFLSNGPGDPEPCDYAIAAIREFLERGIPVFGICLGHQLLALAS
                     GAKTLKMKFGHHGANHPVKDMDTGQVLITSQNHGFAVDADSLPKNLRVTHVSLFDGSL
                     QGIARTDVPAFSFQGHPEASPGPYDVAYLFDRFLDTMTRSAAAQQAAQ"
     gene            1440..>4086
                     /gene="carB"
                     /locus_tag="PLD53_09920"
     CDS             1440..>4086
                     /gene="carB"
                     /locus_tag="PLD53_09920"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRC61274.1"
                     /translation="MPKRTDIKSVLIIGAGPIIIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPITWQVVAKIIEKERPDVLLPTMGGQTALNCALDL
                     DREGVLEKFNVELIGASKEAIDKAEDRQKFKDAMTKIGLGSARSAVAHSMEEAYQVQA
                     AIGFPTIIRPSFTLGGSGGGIAYNMEEFETICKRGLEASPTHELLIEESLLGWKEYEM
                     EVVRDKADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQILRNASIAVLREIG
                     VDTGGSNVQFSINPDDGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     LANDITGGKTPASFEPSIDYVVTKVPRFAFEKFPQADNTLTTQMKSVGEVMAIGRTFQ
                     ESLQKALRGLEVGVDGFNLKSVDPETIDEQLARPTPDRLWYVADAFGVGMSVEKVFDL
                     TKIDPWFLVQIKELVDLELEIEKRDFSTLTAEELRFLKRKGFADRRLAHLTKTSETAV
                     RERRHELGVRPVYKRVDTCAAEFSTGTAYMYSTYEDECEAKPTDKKKIMVLGGGPNRI
                     GQGIEFDYCCVHAAMAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIV
                     AIEKPVGVIVQYGGQTPLKLALALEANGVPIIGTSPESIDIAEDRERFQKLLHDIGLK
                     QPPNRTARTEADALRLAVEIGYPLVVRPSYVLGGRAMEIVHEQKDLERYMREAVKVSH
                     DSPVLLDRFLNDACEVDVDALSDGVEVIIGGVMEHIEQAGVHSGDSACSLPPYSLSKA
                     VQDELRRQTKLMAKALNVCGLMNVQFAIKDEIVYVLEVNPRASRTVPFVSKATGMQLA
                     KIAARCMAGRSLKDQGIAGEVVPPYF"
BASE COUNT          877 a         1044 c         1190 g          975 t
ORIGIN      
        1 tggaaatcga aaatgccgca ctatcagttg aaaatacgga ttgggcgaat tgccaccggc
       61 tctacgctga gctggcggaa gagatgctac agattcgccc cggtattgaa caattcgtca
      121 agacacgggt gcttccgtaa tcccttgaat ttgcctgaaa agctgatttt cggctagaat
      181 aataaggtta aaaaagcaca agcggggagg cgcgagcctt cccgcttggc acatgcacaa
      241 atagaatttt aggagagccg ttcgtgtcgt tgttgccctc attcataccc gccattctcg
      301 ccctcgccga cggaacggta ttcaagggcc agtccatcgg cgcagatggc gtgaccagcg
      361 gcgaggtggt attcaatacc gccatgtccg gctatcagga aatccttacc gatccatcct
      421 attgtcgcca aatcgtcacg ctgacttacc cgcatatcgg taacaccggc tgtaacgcgg
      481 aggacttcga gtctgatgcc aattacgccg ccggtctggt tattcgtgac ttgccgccgc
      541 gtgcgtccaa ctggcgcatg caggaggatc tttcctcata cctcaaaaaa cacgggattg
      601 tagcgattgc gggtatcgat acccgtaagc tgacacgtat tctccgtgaa aaaggtgctc
      661 aggctggctg catacttgcc ggtgaagttg atgaatccag ggcgttagcg ctggccaacg
      721 acttccctgg cctggctgga atggatctgg ccaaggttgt ttcggccaag gcgggctaca
      781 cctggagcga aggtgagtgg acgctgaatg gctacaagcc gggccctgag ccacgttttc
      841 atgtggttgc ttatgatttt ggcgttaagc ggaatattct tagaatgctc gcccagcgcg
      901 gttgcaagct aacggttgtg ccggcgcaaa cgccggccgc tgatgtgctg gcgatgaatc
      961 cggacggtat tttcctgtca aacggccctg gtgatccaga accctgtgat tacgcgattg
     1021 ctgccattcg cgaatttctt gagcgcggca ttcctgtttt cggtatctgt ctcggtcatc
     1081 aactgttggc gttggcttcg ggtgccaaaa ctctgaagat gaaattcggt caccacggtg
     1141 ccaatcatcc ggtcaaggat atggataccg ggcaggtgtt gatcaccagc cagaatcacg
     1201 gctttgcggt cgacgccgat tccttgccaa aaaatctgcg ggtgactcac gtttccctgt
     1261 ttgacggttc cttgcagggt attgcccgca ccgacgtgcc tgccttctcg ttccaggggc
     1321 atccggaagc cagtccgggg ccttacgatg tggcttatct ttttgatcgc ttcctcgaca
     1381 ccatgacgcg cagcgctgct gcgcagcaag cggcgcaata aggcgccgca ggaataacca
     1441 tgcctaaacg tactgacata aaaagtgttt tgattattgg tgccggcccg atcattattg
     1501 gtcaggcctg cgaattcgat tactccggcg cccaggcttg caaggctttg cgcgaagagg
     1561 gttataaggt cattctggtc aattcgaatc cggcgaccat catgaccgat ccggaaatgg
     1621 ctgatgtcac ctacatcgag ccgattacct ggcaggtcgt cgccaagatc atcgaaaaag
     1681 aacgcccgga tgtgttgctg ccgacgatgg gtggccagac cgcgctgaac tgcgcgctcg
     1741 atctggatcg tgagggcgtg ctggaaaaat tcaatgttga gctgattggt gcctccaaag
     1801 aggctatcga caaggcggaa gatcgccaga agttcaagga tgcgatgacc aaaattggtc
     1861 tcggttctgc tcgctcggct gtcgcgcata gtatggaaga ggcgtatcag gtgcaggccg
     1921 cgattggttt tccaaccatt attcgcccct ctttcacgct cggtggttcc ggtggtggta
     1981 tcgcctacaa catggaagag tttgagacca tctgcaagcg cggtcttgaa gcttcgccga
     2041 cgcatgagtt gttgatcgaa gagtccttgc tcggctggaa agagtacgag atggaagtgg
     2101 ttcgcgacaa ggcggacaac tgcattatcg tgtgctcaat cgaaaatctg gatccaatgg
     2161 gcgtgcatac gggtgactcg atcactgtcg ccccggctca gaccttgacc gacaaggaat
     2221 accagattct gcgtaatgct tcgatcgctg tgctacgcga aatcggggtt gatacgggag
     2281 ggtctaacgt ccagttctcg atcaatcctg atgatggtcg catggttgtt atcgagatga
     2341 atccgcgtgt ctcgcgttcg tcggcgctgg cttccaaggc gactggtttc ccgattgcca
     2401 agattgccgc caagttggcc attggctaca cgctggatga actggccaac gacatcaccg
     2461 gcggcaagac gccggcttca ttcgagccgt ccatcgacta cgttgtgacc aaggtgccgc
     2521 gtttcgcttt cgaaaagttt ccgcaggctg ataacaccct gaccacgcag atgaaatcgg
     2581 tcggtgaagt gatggcgatt ggtcgtacct tccaggagtc gctgcagaaa gccttgcgcg
     2641 gtctggaggt cggtgtcgat ggttttaatc tgaaatcagt ggatccggaa actatcgatg
     2701 aacaattggc ccggccaacg ccggatcgcc tgtggtacgt tgctgatgcc ttcggtgtcg
     2761 gcatgtcggt cgagaaagtc tttgacctga cgaagattga tccgtggttc ctggttcaga
     2821 tcaaggagct cgttgacctt gagctggaaa tcgaaaaacg tgatttttcg acgttgaccg
     2881 cagaagagct gcgtttcttg aagcgcaaag gttttgctga ccgtcgtttg gcgcatctga
     2941 ccaaaactag tgaaaccgcg gtgcgtgagc gtcgccacga gctcggcgtt cgtccggtct
     3001 acaagcgcgt cgatacctgt gcggccgagt tctcgaccgg tacggcgtat atgtactcga
     3061 cctacgagga tgagtgcgag gccaagccga ccgacaagaa aaagatcatg gtgctgggcg
     3121 gtggaccgaa ccgtatcggt cagggtattg agtttgacta ttgctgcgtt cacgcggcga
     3181 tggcgatgcg tgaagatggt tacgaaacca tcatggtgaa ctgcaacccg gaaaccgttt
     3241 ctaccgatta cgacacctcg gatcgtctgt atttcgagcc gctgacgctg gaagatgtgc
     3301 tggaaatcgt tgccatcgaa aagcctgttg gtgtcatcgt tcagtacggc ggccagaccc
     3361 cgctcaagtt ggcgttggcg ctcgaagcga acggcgtgcc gatcattggc acttcgccgg
     3421 agtcgatcga tatcgccgaa gatcgcgaac gctttcagaa gctattgcat gatatcgggc
     3481 taaaacagcc gcctaaccgt actgcgcgca ccgaagctga tgcgttgcgc ctggctgttg
     3541 aaatcggcta tccgctggtt gtccgtccgt cctatgtgtt gggtggtcgg gccatggaaa
     3601 tcgttcatga gcaaaaagat cttgagcgat acatgcgcga agctgtcaag gtttcccacg
     3661 attcgccagt tttgcttgac cgttttctga atgacgcttg cgaagttgac gttgatgcgc
     3721 tgtcggatgg cgttgaggtg atcatcggtg gtgttatgga gcatattgaa caggctggcg
     3781 tccattcggg cgactcggct tgctcgctgc cgccctattc cctgagcaag gcggtgcagg
     3841 atgaattgcg ccgtcagaca aaactgatgg ccaaggcgct gaatgtgtgc ggtctgatga
     3901 atgtccagtt cgccatcaag gatgaaattg tctacgttct tgaagtgaat ccgcgtgcct
     3961 cacgcaccgt gcccttcgtt tccaaggcaa cgggcatgca gttagccaag attgcggcac
     4021 gctgcatggc gggtcgtagt ctgaaagacc aagggattgc tggcgaggtt gtgccgcctt
     4081 atttct
//
LOCUS       DAORPM010000221         4065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6869_length_4065_cov_9.493766, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000221 DAORPM010000000
VERSION     DAORPM010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6869_length_4065_cov_9.493766"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..1000)
                     /locus_tag="PLD53_09925"
     CDS             complement(320..1000)
                     /locus_tag="PLD53_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61275.1"
                     /translation="MIDITPEGFGIPISIPSEYGLLHGQLALLPNAHGLVVLAHAAMA
                     LDARDELLAGRFRHAGLSTLSVDLLARQEERFADVHNNVPLLAKRLLDFLGLIRNRML
                     LGELQTLPLGLYAANATSPVAVRVAAQRDHGISAIVCRGGLIDLAGVLYLRSLESPLL
                     MLVEETDTQHIASSNRALKEVHGCKQLKLIPEIGLEYVSSTGFEVSAHEASLWFVRHF
                     TEAFPVPT"
     gene            complement(997..2109)
                     /gene="amrS"
                     /locus_tag="PLD53_09930"
     CDS             complement(997..2109)
                     /gene="amrS"
                     /locus_tag="PLD53_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRC61276.1"
                     /translation="MERGLKTPESNFPARWWHALPHEEGKPARIQCDLCPRDCKLHEG
                     QRGACFVRQNIADQMVLTTYGRSSGFCIDPIEKKPLNHFYPGSSILSFGTAGCNLACK
                     FCQNWDISKSKDMDRLMDAATPDGIASAAAAYGAQSVAFTYNDPVVFAEYAIDTALAC
                     HEKGIKTVAVTAGYIHPEPAREFYAVMDAANVDLKAFTDEFYVKLCGGHLQAVLDILA
                     YIHHETDCWLEITTLLIPSQNDSPAEIKALSEWVASELGADVPLHFTAFHPDWKMGDL
                     PPTPPSSLTRARRIALDAGLHYVYTGNVHDTEGGTTFCPACHAALIERDWYNIRRFDL
                     PDDGSCPHCQAKIAGRFGKFGKPFGAQRIPVRLARR"
     gene            complement(2094..2642)
                     /gene="amrA"
                     /locus_tag="PLD53_09935"
     CDS             complement(2094..2642)
                     /gene="amrA"
                     /locus_tag="PLD53_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRC61277.1"
                     /translation="MPTDALGSSLLTLARNAIGQRFGVGEQPVAAHPSLQEPGATFVT
                     LTQQGQLRGCIGSLEAHRPMAADVAENAVAAAFRDPRFPPLSEDELARTRVEVSLLEP
                     SEALTFADEDDALARLRPGVDGLILKHGSRRATFLPQVWESLPDRRRFLEQLKLKAGL
                     PADFWDGQITLARYGVQKWKEA"
     gene            complement(2632..3441)
                     /gene="amrB"
                     /locus_tag="PLD53_09940"
     CDS             complement(2632..3441)
                     /gene="amrB"
                     /locus_tag="PLD53_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRC61278.1"
                     /translation="MSAKLRPAAVAGMFYPGSATALSRDISQMLSLVAEKRMSRPKAI
                     IAPHAGYIYSGPVAASVYAPLAALRGSIRRIVLLGPTHRFAVNGLALPASTAFATPLG
                     AVPVDQQAVAAIEDMPQVVVSDAAHAQEHSLEVQIPFLQVVFGTFSLLPLAVGHASAE
                     AVCEVLERLWGGDETLIVISSDLSHYLPYDAARQVDAGTARRILDLHYAIDHEMACGA
                     TPINGLLLAARKHGLQAQLLDLRNSGDTAGDKSRVVGYGAFAFHKAEYDAH"
     gene            3749..>4065
                     /locus_tag="PLD53_09945"
     CDS             3749..>4065
                     /locus_tag="PLD53_09945"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRC61279.1"
                     /translation="MESFGTDDTLVDFVITRLPCRIGRGKENDLVIANLGLSRFHAVL
                     VRDISGQIRLIDENSTNGTFVNRRRIDGYCLLNANDIIHFASAEFKLRLSLVDQSLMQ
                     PFD"
BASE COUNT          834 a         1164 c         1271 g          796 t
ORIGIN      
        1 ttccggtagt gctgccatgt ttttgtctcc tagcagaagg gggcttgccg ttcaccaaag
       61 ctgtgaattt gtctggcaag ggtagtacgt tgctgcaatg caaagtaccg cagtgcaaca
      121 aatggtggtg gtgattccag ctgcgcacaa cgatgtgttg gtggtgttgc aacgcacgat
      181 ataaataaac ggtaagtatc gaccagctaa cccggctcta agttcaactg tattgttgag
      241 gtcagaatcg gcaaacccgg aatcgccgct tgcgatgcag tgatacggcg atcggagttt
      301 gcatcggccc cgaagcgctt caagtcggca ccggaaaagc ctcggtgaaa tgtctgacga
      361 accacagcga tgcttcgtga gcagaaactt caaagccggt tgagctgacg tactcgaggc
      421 cgatttccgg aatgagcttg agttgcttgc agccgtgcac ttccttcagc gcccggttgc
      481 tgcttgcgat gtgctgcgta tccgtttctt cgaccagcat gagcaagggc gattcgagcg
      541 agcgtagata gagcacgccg gccagatcga tcaatccgcc ccggcagaca atcgcggaaa
      601 tgccgtgatc gcgctgggcg gcgacgcgaa cggcgacggg tgaagtggcg ttagcggcgt
      661 acaggccgag cggcaacgtc tggagctcgc cgagcagcat gcggtttctg atcaggccaa
      721 gaaaatccag cagtcgtttg gcgagcagcg gaacgttgtt gtgaacatcg gcaaagcgtt
      781 cttcctgtcg ggccagcagg tccacactca gcgtcgatag cccggcatga cgaaaccgcc
      841 cggccagcaa ttcatccctg gcatccagcg ccattgcggc atgcgccaga acgaccaagc
      901 catgggcatt gggcaacaga gccagttggc cgtgcagcag gccgtactcg gaagggatcg
      961 aaatcgggat gccaaaacct tcgggagtga tgtcgatcat cttcgcgcaa gcctgactgg
     1021 aatgcgctgc gcaccgaatg gcttcccgaa cttcccgaaa cgcccggcta tctttgcctg
     1081 gcaatgcggg caagaaccat cgtccggaag atcgaaacgg cggatgttgt accagtcgcg
     1141 ctcgatcagc gcggcgtgac aggcggggca gaaggtcgtg ccgccctctg tgtcgtgcac
     1201 gttgccggtg tagacgtagt gcagtccggc gtcgagggcg atgcggcggg cacgggtaag
     1261 agatgacggt ggagtcggcg gcagatcgcc cattttccag tcgggatgaa atgcggtgaa
     1321 gtgcagcgga acatccgcgc ccaactcgct cgcgacccat tcggacaagg ccttgatttc
     1381 cgcaggcgaa tcgttctggc tggggatcag cagggtcgtt atttccagcc agcaatcggt
     1441 ttcatggtgg atgtaagcga gaatatcgag aacggcctgc aggtgaccgc cgcagagctt
     1501 gacgtagaac tcgtcggtaa aggccttgag atcgacattg gccgcgtcca tcacggcgta
     1561 gaactcccgc gccggttccg gatggatgta gccggcggta acagcgactg tcttgatgcc
     1621 tttttcgtgg caggccagcg ccgtgtcgat ggcgtattcg gcgaaaacga ccgggtcgtt
     1681 ataggtgaag gccaccgatt gcgcgccata ggctgccgcc gcactggcga taccgtccgg
     1741 ggtggcggcg tccatcaggc ggtccatgtc cttcgatttc gagatgtccc agttctggca
     1801 gaacttgcac gccagattgc agccggccgt gccgaacgag agaatcgaag atccggggta
     1861 gaaatgattg agcggcttct tttcgatcgg gtcgatgcag aagccggacg agcggccgta
     1921 ggttgtcagc accatttgat cggcaatgtt ctggcgaacg aagcaggcgc cgcgctggcc
     1981 ttcgtgcagt ttgcaatccc gcgggcagag atcgcactgg atgcgtgccg gtttgccttc
     2041 ctcgtggggc agggcgtgcc accagcgtgc cgggaaattg gattccggag ttttcaggcc
     2101 tctttccatt tttgcacacc gtagcgggca agcgtgattt gcccgtccca gaagtcggcc
     2161 ggcaacccgg ccttgagttt gagctgttcc aggaatcgcc ggcggtcggg cagcgattcc
     2221 cagacttgcg gcaggaaagt ggcgcgtcgg ctgccgtgct tcagaatcag cccatcgacg
     2281 ccgggtctca ggcgggcgag ggcatcgtcc tcgtctgcaa acgtcagcgc ttccgacggt
     2341 tcgagcaagg agacttcgac tcgcgttcga gccagttcat cctcgctcag cggtggaaag
     2401 cgcgggtcgc gaaaagcggc ggcaacggca ttttcagcga cgtcggccgc catcggacgg
     2461 tgggcttcca gactgccgat acagccgcgc agttgcccct gctgcgttag cgtgacaaag
     2521 gtcgcgcctg gctcctgaag cgacggatgc gcggcgacag gctgttcccc cacgccgaag
     2581 cgctggccga tggcgttgcg cgccagggtc agcaggctcg atccgagtgc atcagtgggc
     2641 atcgtactct gccttgtgaa aggcaaacgc gccgtagccg accacgcgcg acttgtcgcc
     2701 ggcagtgtcg cccgaattgc gcaggtcgag caactgcgct tgcaaaccat gcttgcgggc
     2761 tgcgagcaac aggccgttga ttggcgtcgc gccgcaggcc atctcgtgat cgatcgcata
     2821 gtggagatcg aggatgcgcc gcgcggttcc ggcatcgacc tgccgcgccg cgtcataggg
     2881 cagatagtgc gagaggtcgg agctgatgac gatcagtgtc tcgtcgccgc cccacaggcg
     2941 ttcaagcact tcgcataccg cttcagccga ggcatgaccg acggccaatg gaagcagcga
     3001 gaacgttccg aacaccacct gcaagaaggg gatctgcact tccagcgaat gctcctgggc
     3061 atgtgcggca tcgctgacga caacctgcgg catatcttct atcgccgcaa ccgcctgctg
     3121 atcgacggga acggcgccaa gcggcgtcgc gaaagccgta ctcgccggca gagccaaacc
     3181 attgactgca aaccgatgcg tcggcccgag cagaacgatg cgccgtatcg accctcgcaa
     3241 ggcggcgagc ggggcgtaaa cgctcgcggc gaccggaccc gaatagatgt aaccggcgtg
     3301 cggagcaatg atcgccttgg gccgcgacat ccgtttttct gccaccaggg acaacatctg
     3361 cgagatatct cggctcaggg cagtcgccga tcccggatag aacatcccgg cgacagcagc
     3421 agggcgtaac ttggcactca tgactggact cctctattaa cactatatga gccggagtca
     3481 ggcaaggcaa gttcaggttt caggtcctgc tatgcgtatt ttgccgcccg atgcagtgtc
     3541 acgcgctctg tccggttgtc atggaaatcg gagcgaatgg tcagtcgttc cgggtagatc
     3601 ggttgcacgg acaacaaccg ttgcatacag gctacaaaag agctgcgtga cgttgatcta
     3661 cctgcagctt gtttgtcacc gcttcaaata ttccaatata atcgcgcaac gctacaacgg
     3721 gagcgatgct ttggaaaagc agtggtcttt ggagtccttc ggaaccgacg atactcttgt
     3781 cgacttcgtg attacccgac tgccatgccg aatcggccgt ggcaaggaga acgatctggt
     3841 gattgcgaac ctcgggctga gtcgtttcca tgccgtgctg gtccgggaca tcagcggcca
     3901 aatccggctc atcgacgaaa acagcaccaa cggcaccttc gtcaatcgtc gtcgtatcga
     3961 cggttactgc ctgctcaacg cgaacgacat cattcatttt gccagcgccg agttcaagct
     4021 gcgcctgagt ctggtcgacc agagcctcat gcagccgttc gacga
//
LOCUS       DAORPM010000222         4046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6926_length_4046_cov_8.229266, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000222 DAORPM010000000
VERSION     DAORPM010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6926_length_4046_cov_8.229266"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLD53_09950"
     CDS             <1..460
                     /locus_tag="PLD53_09950"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRC61280.1"
                     /translation="PLPTLEAAAALCLELGLMANVEIKPAAGFESVTGEVVAKLIPQL
                     WRGAPLPLVSSFSDEALIAARNAAPLVPLGCLWERLPSNWLDRLNALGAYSLHCATSE
                     LDDATLGTASANGIPVLCYTVNDRHAADTLFKRGVAAVFSDRIDKFRITS"
     gene            677..1048
                     /locus_tag="PLD53_09955"
     CDS             677..1048
                     /locus_tag="PLD53_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61281.1"
                     /translation="MKLLKSTLISLVLISPLSSLAQSNNTPKFDQRQANQERRIQQGE
                     QSGSLTQREAERLNKGQDHLQTVEEKAKADGTVTRQERARLQHAEDKQSVRIYRQKHD
                     RQHDYNHNGKPDRPHRGGFPS"
     gene            1239..1667
                     /locus_tag="PLD53_09960"
     CDS             1239..1667
                     /locus_tag="PLD53_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61282.1"
                     /translation="MGMLDKLFGGKKDYPPLPTGDEAHARIDMVKGPLEDLAHRISDN
                     MEVVPSEQEAFVFLGKPPKRFGIAWIHDGKVSGLKELADENKLSPIDVGKIISNLGEA
                     YEHASDSPRFSLDVGGKQAVVIPSKGLEQDVRRIISNAIH"
     gene            complement(1715..2725)
                     /gene="holA"
                     /locus_tag="PLD53_09965"
     CDS             complement(1715..2725)
                     /gene="holA"
                     /locus_tag="PLD53_09965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRC61283.1"
                     /translation="MQIKGEQLAVHLEQALQAVYVVYGDEPLLVLEAGDAIRSAARRN
                     GFDEREVLTVIPGFNWIALHHAAGNMSLFGGRKLIDLRIPTGKPGREGSSAIQDYCTH
                     PSPDTLLLVTLPGLDWTEEKAAWLKALAAAGVVVKLIPPNPAELPAWISGRLRRQQQS
                     VESDGLRFIAERVEGNLLAAHQEILKLGLLYPAGEVTLEQVQQAVLNVARYDLDGLRE
                     ALLVGDVTRLTRTLDGLQQEGEAPPLVLWAMTEEVRALAQVRTGLTQRKSLDALLKDA
                     RVWGPRQARFKRALKRIDTARASAALAHAARIDRMIKGAGGSGDVWDEFLRLGLSIGV
                     NL"
     gene            complement(2732..3244)
                     /gene="lptE"
                     /locus_tag="PLD53_09970"
     CDS             complement(2732..3244)
                     /gene="lptE"
                     /locus_tag="PLD53_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRC61284.1"
                     /translation="MRSRLRSTLLVLCVAVIAGCGFQLRGSYTLPYESLYVAVPDTSL
                     IGANLKRAIRAADSTRLADSAADAQATFLPTGELREPVILSFSGSGRVREKRLRYRYG
                     YRVVDSKGRNLILPGVIELNRDLTYADSDVLAKTQEEELLWRDMESDLVQQLMRRLAA
                     TKPVYPATAK"
     gene            complement(3264..>4046)
                     /locus_tag="PLD53_09975"
     CDS             complement(3264..>4046)
                     /locus_tag="PLD53_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041224677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC61285.1"
                     /translation="VLRADGLPVVIGGIEKMSKSRNNGVDPQALIDQFGADTARLFIM
                     FASPPDQSLEWSDAGVEGAFRFLKRVWRIVFEHVSGGLVAAFPGGEASEQLPEELKTF
                     RRQLHQAIGKITDDYGRRKQFNTAIAAVMELLNAYAKLVDESATARAVRQETLEAVSL
                     LLYPIVPHVCEALYAELRPGQMAGVQPFPKVDAAALVQDEIELMLQVNGKLRGSLRVP
                     AGADKTAIEAATLASEVAQKHLAGKAPRKLVVVPGRLVNIVV"
BASE COUNT          897 a         1222 c         1106 g          821 t
ORIGIN      
        1 gccgttacca acgctcgaag ctgcggcggc cctctgcctg gaactcggcc tcatggccaa
       61 tgtcgagatc aagccggccg ccggatttga atcagtcacc ggcgaagtcg tcgcaaaact
      121 tattcctcaa ctgtggcgtg gcgccccgct accgctcgtt tcgtcatttt cggatgaagc
      181 gctgatcgca gcacggaacg ccgctcctct cgtgcctctc ggctgtctgt gggagcgcct
      241 cccgtccaac tggctcgatc gcttgaatgc tttgggagct tacagcctgc attgcgcgac
      301 cagcgagctt gacgatgcca ctcttggtac cgcgagcgcc aatggcattc ccgtgctttg
      361 ttacacggtc aatgatcgac atgcggccga tacgctgttc aagcgtggcg tggcggcagt
      421 attcagcgac cgcatcgata aattccgtat cacgtcatag tcgccgcttg atattcagca
      481 cgtgcagcgc ccgccgaatg ctacgcgatg ctcatacggc tgacatttcc agtctgattt
      541 cgattagttg taagagtgta ttgctgctta atggcttgta acaaaaagcc gacttccctt
      601 gttcgagacc atgcgtaaat tcatccgtgg gacaacccat gtcaattttc gcaaatatca
      661 ataggaggtc ggaaccatga agctgctcaa atctacgctt atttccctcg tgctgatttc
      721 accactgtcg tctcttgcac aatccaacaa cacgcctaaa ttcgatcagc gccaagccaa
      781 tcaggaacga cgtattcagc agggagaaca atccggctcg ctgacacaga gagaggccga
      841 gcgactaaat aaaggccagg atcatttgca gacagtggaa gaaaaagcca aagccgatgg
      901 cacagtcacg cgccaggagc gggcaaggct tcagcatgcc gaggataagc aaagcgtaag
      961 gatatacaga cagaaacatg accgtcagca cgattacaac cacaacggca agcctgaccg
     1021 gccgcatcgt ggcgggtttc cgtcttaacg gtgatgacgc tagaacgctt gcaggccggg
     1081 cgattgagga atttcggctc aagcgggaaa gcccggcagg tttcaataat ccttgaaaat
     1141 ccaccaatcg cgtctaggct tagattgcat gtcagcgcat ggcaatccaa tatttggagt
     1201 gtgttgactc tttatttatc cgcgacaagg aggacatcat gggaatgcta gacaagttgt
     1261 ttggtggaaa gaaggactac ccaccgctac cgactggcga cgaggcacac gccagaatcg
     1321 acatggtaaa aggcccgctg gaagacttgg cccatcgcat ctccgacaac atggaagtag
     1381 ttccgtcgga gcaggaagct ttcgtttttc ttggcaaacc acccaaacgt tttggtattg
     1441 cgtggattca tgacggcaaa gtgagcggcc ttaaagaact ggcagacgaa aacaaactct
     1501 cgccgattga tgttggcaaa atcatcagca atctgggcga agcttacgag cacgccagcg
     1561 attcaccgcg tttctcattg gacgtgggtg gaaagcaggc cgtggtaatc ccttcaaagg
     1621 ggttggagca ggatgttcgt cggattatct cgaacgcaat ccactagact tcgaccggcg
     1681 tggtcgggtt cgcagccgcg aatctggccc cgctttacag gtttacaccg atactcaggc
     1741 cgaggcggag aaattcatcc caaacatctc cagacccgcc cgcgcctttg atcatccggt
     1801 cgatgcgtgc cgcatgcgcc agtgcggcgc tggcccgcgc agtgtcgatt cgtttcaggg
     1861 ctcgcttgaa ccgtgcctga cgtggccccc atacgcgcgc gtccttgagc aacgcatcga
     1921 gtgacttgcg ctgcgtcagc cccgtcctga cctgtgccag agctcgcact tcttcggtca
     1981 tcgcccagag aaccagtggt ggtgcctcgc cctcctgctg caggccgtca agggtacggg
     2041 tcaggcgagt cacatcaccg accaggagcg cttcacgcaa tccgtcgagg tcgtagcggg
     2101 cgacattgag caccgcctgc tgcacctgct ccaaggtgac ttcgccggcc ggatagagca
     2161 ggccaagttt caggatttcc tgatgggcgg ccaacagatt gccttcgacg cgttcggcaa
     2221 tgaagcgcag cccgtcgctt tccacgctct gctgctggcg gcgcaagcgc ccggaaatcc
     2281 aggccggcag ctcggccgga ttcggcggaa tcaacttgac caccacgcca gcagccgcca
     2341 atgctttcag ccaggcggcc ttttcttctg tccagtcaag cccgggaagc gtcaccagca
     2401 acaaagtgtc tggtgacggg tgtgtgcagt aatcctgtat tgccgaacta ccctcgcgcc
     2461 caggcttccc cgtcggaatg cgcaggtcaa tcagcttgcg cccaccgaac agcgacatat
     2521 tgcccgccgc atggtgcaat gcgatccagt tgaaccccgg gatgaccgtg agtacctcac
     2581 gctcgtcaaa accatttcgg cgagccgccg aacgaatggc atcgccagcc tcaagcacca
     2641 acaggggctc atcaccgtat acgacatata ccgcttgcag cgcctgttcc agatgaactg
     2701 caagctgctc gcccttgatc tgcatgacgg cctacttggc agttgccgga tacaccggtt
     2761 ttgtggccgc cagtcggcgc atcaattgct gcaccagatc gctttccata tcccgccaga
     2821 gcagctcttc ttcctgggtt ttcgccagca cgtccgagtc ggcataggtc agatcacggt
     2881 tcagttcgat cactccgggc aagatcagat tccgcccctt gctatccacc acacggtagc
     2941 cgtaacgata gcgcaacctt ttttcacgca cgcgaccgga accggagaat gaaagaataa
     3001 ccggttcacg caactcgccg gtgggcaaga aggtcgcctg cgcatccgcg gcagaatcag
     3061 caagccgagt cgagtccgcc gcacgtatgg cacgcttgag gttggcacca atcagtgaag
     3121 tgtcgggcac agcgacgtaa agactctcgt aaggcagggt ataggaaccg cgcagttgaa
     3181 atccgcagcc ggcaattacc gccacgcaca gcacaagcag ggttgaacga agccttgaac
     3241 gcatttcggc attcctccgg gtattacacg acgatgttaa ccaaacgacc gggcaccacg
     3301 accagtttcc tgggcgcctt tccggcgaga tgtttctgcg ctacttcgga agccagcgta
     3361 gccgcttcga tggccgtctt gtcggcgcca gccggtacgc gcaaactgcc gcgcagcttg
     3421 ccgttgacct gcagcatcag ctcgatctcg tcctgcacaa gcgccgccgc atcgactttc
     3481 gggaaaggct gcacgccagc catttggccg ggcctgagtt cggcgtaaag cgcttcgcag
     3541 acatgcggca cgatcgggta gagcagcagc gacacggctt ccagcgtttc ctgccttact
     3601 gctcgcgcag tcgccgattc atcaacgagc ttggcatagg cgttaagcaa ttccatcacg
     3661 gcggcaattg cggtattgaa ttgcttgcgt cgaccgtaat catccgtgat cttgccgatc
     3721 gcctgatgca gctggcgacg gaaagtcttc aattcttccg ggagttgttc agacgcctcc
     3781 ccgcccggaa atgccgcaac aagaccgccg gaaacatgct cgaagacgat tcgccagacg
     3841 cgcttgagga agcggaacgc gccttcgacg ccggcatccg accactccag cgactgatcc
     3901 ggcggcgagg caaacatgat gaacagccgt gccgtatcgg caccaaactg atcgatcagg
     3961 gcctgcgggt cgacgccgtt attcctcgat ttcgacattt tctcgatgcc gccaatgacc
     4021 accggcaggc cgtcggcccg cagcac
//
LOCUS       DAORPM010000223         4037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_6955_length_4037_cov_10.523857, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000223 DAORPM010000000
VERSION     DAORPM010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6955_length_4037_cov_10.523857"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLD53_09980"
     CDS             complement(<1..295)
                     /locus_tag="PLD53_09980"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRC61286.1"
                     /translation="MALLSWSNQYLIGNDLIDTEHEELFRLINNFHSLWMEKQNRQDI
                     ARVLNQLIAYAQMHFQHEEVIMEEAGYPKLAEHQQIHEDMIEKIFELHTSYAEG"
     gene            complement(326..1876)
                     /gene="lnt"
                     /locus_tag="PLD53_09985"
     CDS             complement(326..1876)
                     /gene="lnt"
                     /locus_tag="PLD53_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRC61287.1"
                     /translation="MVPPVPRLIIALLLGAASVLAYAPAGWFPLIWLTLGGLYILLDG
                     RASENGGSFKNAFAGGLLGASFGFGLFIAGVSWVYVSLSVFGGMPGAMAALATFLFCC
                     VLSIFPALAGALFFRFSPNGWWQRSLYFACLWTLSEWLRGWVLTGFPWLAVGYSQAPP
                     SPLSGFAPVLGVYGATLMTALLAALISEALRRWVPVEARACSRNTWTKPARWRAVLPI
                     LIAVLLLAGGNALRNVRWTSPKGESLSVALLQGNVAQDMKWRPEKFSESLRIYYRLAL
                     ENPAQLTVLPETALPAFLGQVPGEYLDELKRLAERQRGDMLLGIAIGDSTRYANGAIS
                     LGQSEEQHYSKTHLVPFGEFVPPGFAWFLALANIPMSDFTPGAKLQVPMSIAGQKVAV
                     NICYEDAFGEEIIRALPEATLLVNLSNVAWFGDSLAPAQHLQIAQLRALETGRMMLRA
                     TNTGMTAIVDAGGHVQSALKPFTRAALLGEVQGYEGATPYVRWGNWPVVSFALLLAAF
                     FGRRNAVS"
     gene            complement(1877..2632)
                     /locus_tag="PLD53_09990"
     CDS             complement(1877..2632)
                     /locus_tag="PLD53_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRC61288.1"
                     /translation="MELLHSAYERNLLDADALSITEGALAASEISVSDVMVPRSQMEV
                     IDIDDPQSEIIAQVNRTAHSRFPVIDGDRDNVIGLLLAKDLLRLQTDVKFNLRDWLRP
                     AVFIPESKRLNMLLREFRVSHNHMAVVVDEYASVCGLITIEDVLEQIVGDIEDEYDFD
                     EADDNIRLDHAGRYRVKAQTGIDDFNEHFGTNFSDEECNTVGGLIMNHLGVVPKRGES
                     IEFGGFLFQVLRADSRRLYTLLVSPLPDTDEPA"
     gene            complement(2723..3184)
                     /gene="ybeY"
                     /locus_tag="PLD53_09995"
     CDS             complement(2723..3184)
                     /gene="ybeY"
                     /locus_tag="PLD53_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRC61289.1"
                     /translation="MTSRRLNLSVQYVCKKAGLPLRPLVRACARAALNVNGVRGGQIT
                     VRFVGSEEGQLLNRDFRGKDYATNVLSFPYATEPIVCGDIVICTPVVEREAGEQGKAT
                     EAHYAHLIVHGILHLQGYEHETSKREARLMEQHECAILAALGYSNPYSENT"
     gene            complement(3181..>4037)
                     /locus_tag="PLD53_10000"
     CDS             complement(3181..>4037)
                     /locus_tag="PLD53_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRC61290.1"
                     /translation="IARRGERFTLRGESIQTARASEALQRFYALAKDTLSVDEIQLGL
                     IELINRPARSDALCGGLSPALMTRKTELHGRTPRQVEYLKHIQEHDITFGTGPAGTGK
                     TYLAVASAVDAIERELVQRIVLTRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDL
                     MGFDRVAKLFERGAIEIAPLAYMRGRTLNHAFIILDEAQNTTPEQMKMFLTRIGIGAK
                     AVVTGDVTQIDLQRGQKSGLIEARQILKDVRGIAFTEFQKEDVVRHPLVARIVSAYES
                     HTSSLEGK"
BASE COUNT          834 a         1146 c         1236 g          821 t
ORIGIN      
        1 tgccttcggc gtaggacgtg tgcagttcga aaatcttttc gatcatgtct tcgtggattt
       61 gctggtgctc ggcaagcttc gggtagccgg cctcctccat gatcacctct tcatgctgga
      121 aatgcatctg cgcgtaggca atcagctgat tcaggactct ggcgatgtcc tgacgattct
      181 gcttttccat ccagagcgag tggaagttgt tgatcagacg aaacaattct tcgtgctcgg
      241 tatcgatcag gtcgttgccg atcaaatact ggttcgacca ggaaagcagt gccatggagc
      301 gattctcccg cgtttttcta gtttgtcagg ataccgcgtt acgtctgccg aagaaagctg
      361 ccaacaacag cgcgaagcta accacaggcc agttgcccca acgtacatag ggcgtggcgc
      421 cttcgtagcc ttgaacttct ccaagcaatg cagcacgggt gaaaggtttc agagcgctct
      481 gaacgtggcc gcctgcgtcg acaatggcgg tcattccggt gttggtggcg cgcagcatca
      541 tgcgacctgt ttcgagggcg cgcaactggg cgatctgcag atgttgtgcc ggtgccagtg
      601 aatcgccgaa ccaggcgaca ttggaaagat tgaccaatag cgtggcttcg ggtagcgcgc
      661 ggatgatttc ctcaccgaag gcgtcttcgt aacagatatt cacggcgacc ttttgcccgg
      721 cgatgctcat cggcacctgc agtttcgcgc cgggagtgaa gtccgacatc gggatgttgg
      781 ccagggccag gaaccaggca aatccgggtg gcacgaattc accaaagggt acgagatggg
      841 tcttgctgta atgctgctcc tccgactggc caaggctgat ggcgccgttg gcgtagcgtg
      901 tactgtcgcc gatggcgatc cccaggagca tatcgcctcg ctggcgttca gcgagcctct
      961 tcaattcatc gaggtactcg cccggaacct gtccaagaaa ggcgggcagt gccgtttcgg
     1021 ggaggacggt cagttgcgcc ggattctcca gtgccaggcg gtagtagata cgcagagact
     1081 cgctgaattt ctcgggccgc catttcatgt cctgcgcaac attgccttgc agcagcgcga
     1141 cgctcagcga ctcacccttc ggtgacgtcc aacggacgtt gcgcaatgca ttgccaccgg
     1201 caagcaacag cacggcaatc agtatcggta gtaccgcgcg ccagcgcgct ggcttggtcc
     1261 acgtgttgcg agagcaagcg cgggcttcaa cgggaaccca gcgccgcaat gcctcgctga
     1321 tcagcgccgc gagcagagcc gtcatcaggg ttgcgccgta gacaccgagt acgggggcga
     1381 atccggagag cgggctgggc ggcgcttgtg aatagccgac cgccagccat ggaaagccgg
     1441 taagcaccca gccgcgcagc cattccgaga gcgtccacag gcacgcaaaa tacaggctgc
     1501 gttgccacca gccgttcggc gagaagcgga aaaaaagggc gcccgcaagt gccggaaaga
     1561 tcgacaggac acagcagaat agaaaagtgg ccagcgcggc catggctccg ggcatgccgc
     1621 cgaaaacgct cagactgaca taaacccagg atacgccggc aatgaacaga ccgaatccaa
     1681 aggaggctcc gagtagtccg ccagcgaacg cgttcttgaa cgagccgcca ttttcgcttg
     1741 cacgtccgtc cagcaagatg tacaggccgc ccagcgtcag ccagatcagt gggaaccagc
     1801 ccgccggtgc ataggccagg acgctggctg caccgagcag cagcgcgatg atcaagcgcg
     1861 ggacaggggg caccactcag gccggctcat cggtgtccgg cagggggctg acaagcagcg
     1921 tgtagagcct gcgactgtcg gcacgaagaa cctgaaagag aaagccgcca aattcgatgg
     1981 actcaccgcg ttttggcacc actccaagat gattcatgat taggccgccg acggtattgc
     2041 attcctcgtc gctgaaattc gtgccgaaat gctcattgaa atcgtcgatg cctgtttgcg
     2101 ccttgacgcg ataacgcccg gcatggtcga ggcggatgtt gtcgtcagcc tcgtcaaaat
     2161 cgtattcatc ttcaatgtcg ccgacgatct gttcgagtac gtcttcaatg gtgatcaggc
     2221 cacacacact cgcgtattcg tcgacgacca cggccatgtg attgtgcgat acacgaaatt
     2281 cgcgcagcag catgttcagt cgcttggact cgggaatgaa tacagccggg cgcagccagt
     2341 cacgcagatt gaatttgaca tcggtctgca ggcgcaggag atccttggcc agcagaagtc
     2401 ctatgacgtt gtcgcgatct ccgtcgatga ctggaaagcg cgagtgtgcg gtgcgattga
     2461 cctgggcaat gatttcgctc tgcggatcgt cgatgtcgat gacttccatc tgggatcgcg
     2521 gcaccatgac atcgcttacg ctgatttcgg aggcagcgag cgcgccttcg gtgatcgaca
     2581 acgcgtcggc gtcaagcaga ttgcgctcgt aggccgaatg aaggagttcc atgagttgtt
     2641 ctctatcctc cggcttgcgc aggagcatag aagacagccg ttcaaagaag ccgagtttgt
     2701 taactgggtt gatgtccatg ggtcaggtgt tttctgaata gggattcgag tatccgagcg
     2761 cggccagaat ggcgcactca tgctgctcca tcaggcgagc ttcccgcttg ctggtttcgt
     2821 gttcgtagcc ctgcaggtgc aagataccat gcacgataag atgagcataa tgggcttcag
     2881 tagctttgcc ctgttcgcca gcttcgcgct cgaccaccgg cgtacagatg acgatgtcac
     2941 cgcacacaat cggctcggtg gcgtagggga aggacagcac gttggtcgcg tagtccttgc
     3001 cgcggaagtc acgattcagc aattgtcctt cttcgctgcc gacaaagcgc acagtgatct
     3061 gtccgccgcg cacgccattg acattcaagg cggctcgagc acaggcgcga accagcggac
     3121 gcaaaggcaa tcctgctttc ttgcatacgt attgaacgga aaggttgaga cgcctgctgg
     3181 tcatttgcct tccagcgagc tggtgtgcga ttcataggcg gagacgatgc gggcgaccag
     3241 cggatggcgg acaacgtctt ccttctggaa ttcggtgaag gcgatgccgc gcacgtcctt
     3301 gagtatctga cgcgcttcga tcagcccgct cttctggcca cgttgcagat caatctgcgt
     3361 cacgtcgccg gtgaccacgg ccttggcgcc gatgccgatg cgcgtgagga acatcttcat
     3421 ctgctcgggc gtggtgttct gcgcctcgtc gagaatgatg aaggcatggt tcaaggtgcg
     3481 gccgcgcata taggcgagcg gtgctatttc gatggcaccg cgctcgaaaa gcttggccac
     3541 ccggtcaaag cccatcagat cgtagagcgc gtcgtaaagc ggacgcaggt aggggtcgac
     3601 cttttgcgcc agatcgccgg gcaggaagcc gagccgctcg ccggcttcga ctgctgggcg
     3661 agtcaatacg atgcgctgca ccagctcacg ctcgatagcg tcaacagccg aagctaccgc
     3721 cagatacgtt tttccggtgc cggccggccc ggtgccaaaa gtgatgtcgt gttcctgaat
     3781 atgcttgagg tattcgacct ggcgcggcgt gcggccgtgc agctccgtct tgcgcgtcat
     3841 cagcgccggt gacaggccgc cgcagagcgc atcgctgcgc gccgggcggt tgatcagctc
     3901 gatcagtcca agctggattt catcgaccga aagcgtgtcc ttggccagtg cgtagaaccg
     3961 ttgcagggct tcggaagcgc gtgctgtctg tatcgattcg ccgcgcagtg tgaaacgttc
     4021 gccgcggcga gcgatgg
//
LOCUS       DAORPM010000224         4007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7051_length_4007_cov_6.039980, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000224 DAORPM010000000
VERSION     DAORPM010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7051_length_4007_cov_6.039980"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..978
                     /locus_tag="PLD53_10005"
     CDS             301..978
                     /locus_tag="PLD53_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HRC61291.1"
                     /translation="MKILVVEDEQKTGSYLRQGLMEAGFVVDLARDGLDGLHLALTEA
                     FDLVILDVMLPGIDGWQVLQGIRRAGKEIPVLFLTARDDVDDRVKGLELGADDYLVKP
                     FAFSELLARVRTLLRRGGKAKESDVLRAADLELDLMRRRVTRSGKKIDLTSKEFALLE
                     LLLRRQGEVLPRSLIASQVWDMNFDSDTNVIEVAIRRLRAKVDDCFEPKLIRTVRGMG
                     YVLEISD"
     gene            975..2360
                     /locus_tag="PLD53_10010"
     CDS             975..2360
                     /locus_tag="PLD53_10010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HRC61292.1"
                     /translation="MSSSGRKSVTFRLTLLFASVSTLVLLLLGLLIGSLVERHFEELD
                     MDLLDGKLELLRHAVMKVRSEQELDALTGQLDDLLVGHQGLAVIVLKPGGQILYSTPG
                     AMFPPELLTVDGVQRLRQWTDSKSRAYRGMAAEAPTGIPDAAPAVVAVSTDLIHHAHF
                     MRTFRVALWLVVGFAALLSGFLGWAAVRRGLAPLRDISRSAAGITANRLDQRLSADAF
                     PVELAEVAKTLNEMLARLEKSFRRLSDFSSDLAHELRTPVSNLLTQTQVTLSKTRTRE
                     EYQDVLASNAEEFERLTRMISDMLFLAKSDNSLIVPHRERVDLAAEIGSLFEFYEALV
                     EEKGVTLSRSGEGSVFGDRLMLRRAVNNLLSNAVRHTPKGGVIKAVIDDSDAAIITLS
                     IENTGETIAPEHLVRLFDRFYRANASPQSFGEGAGLGLAITRSIAHAHGGEVLAHSEN
                     GVTRLEIRLPV"
     gene            2572..2937
                     /locus_tag="PLD53_10015"
     CDS             2572..2937
                     /locus_tag="PLD53_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HRC61293.1"
                     /translation="MKNRPNYSTLAVLATGGMLAAGCVVVPGVEVTALPTRIDYACAG
                     GKTLPVARAPEQRMAAVLVNNREILLRAGESAAQEKYTDGQYSLYLDGEKAFLEENGI
                     VLFGPCTSPVPLSKYYRTY"
     gene            complement(3187..3792)
                     /locus_tag="PLD53_10020"
     CDS             complement(3187..3792)
                     /locus_tag="PLD53_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61294.1"
                     /translation="MMLHFIMRIGGDTIGVGKSTPNPLSEPQVFMSLPRAIGYLGITL
                     LLALATFGIFVAFTKHDWYGVFVMVGFLGILAFFWHALRAAAKREQDPALRAASGLGW
                     YGESLSALFRGPVLHTFEGLTVVCGSVISLLYAVLAWFSPSMVALNPARASINTTLFC
                     LWPILLFVVYVRFCGPHFRQSLYTSLVMLCAVGVPFYIAYK"
BASE COUNT          885 a         1106 c         1195 g          821 t
ORIGIN      
        1 ccacgcgatg aggcggtaaa cggttgggct gtggggtgta tcggccgcca cgtccaccat
       61 acccgcctga agcaggacgg ccaccgctgc gaggccaaga acaaggacgc cggatgcgcc
      121 gagcaggatt ttcttcatgg aaaccgtttc aattcaagac gggctgattg tccggtaaac
      181 ggtataacag taagctgact tgcccattac attcttgtaa tcttggtgac ccgatctcac
      241 tatcagcgca gaatcgggcg ctcaagcgaa gttcaagcca agtggtaccg gagtgagagc
      301 atgaagatcc tggttgtgga agacgaacag aagacaggaa gctacctgag gcaggggttg
      361 atggaggccg gtttcgtcgt cgatctggcg cgagacggcc tggatggtct gcacctggcg
      421 ttgaccgagg ccttcgacct ggtgattctg gacgtgatgt tgcctggcat cgacggatgg
      481 caagtgctgc aaggtatccg acgagccggc aaggaaatac cggttttgtt cctgacggcc
      541 cgcgacgacg ttgacgaccg ggtcaagggg ctggaactcg gggctgacga ctatctggta
      601 aagccttttg cattctccga gctgctagcc agggtcagaa ccttgctgcg gcgtggcggc
      661 aaagccaagg agtccgacgt tctccgtgcc gccgatcttg aactcgattt gatgcgccgc
      721 agggttacac ggtccggaaa gaagatcgat ctgacctcga aggagttcgc tttgcttgag
      781 cttctcttgc gacgtcaggg cgaagtcctg ccgcgttcgt tgatcgcctc ccaggtctgg
      841 gacatgaact tcgacagcga caccaacgtc atcgaggtcg ccatcaggcg attgcgggcc
      901 aaggtggatg actgctttga gccgaaactg atacggacgg ttcgcggtat ggggtacgtg
      961 ctggagatca gcgattgagt tcaagcggac gcaaatccgt caccttccga ttgaccctcc
     1021 tcttcgcgtc ggtctcgacg ctggttcttc tcctgcttgg tttgttgatt ggctcgctcg
     1081 tcgagcggca ttttgaagaa ctggacatgg atcttctcga cgggaaattg gagcttctcc
     1141 ggcacgccgt aatgaaagtc cgctcggagc aggagctgga tgcgctcaca gggcagctgg
     1201 acgatttgct ggtggggcac cagggtttgg ctgtgattgt cttgaaaccg ggcgggcaaa
     1261 tcctgtactc gacccccggc gcgatgtttc caccggaact gctgacggtt gacggcgtac
     1321 aacgcctcag gcagtggacc gattcgaaga gccgcgccta tcgaggaatg gcagcagaag
     1381 cgccgaccgg aattccggat gctgccccag cggtcgtggc cgtgtcgacc gacctgattc
     1441 accacgcgca tttcatgcgg acgttccgcg tcgcactctg gctggttgtt ggttttgcgg
     1501 cattgctcag cggttttctc ggctgggccg ccgtgcgccg ggggttggca ccgctgcgcg
     1561 atatcagtcg cagtgcggcc ggcattactg ccaatcgtct cgaccaacgg ctgtcggcag
     1621 acgccttccc cgtcgaactg gccgaggtgg cgaaaacgct taacgagatg ctggcgcgac
     1681 tcgagaaatc gtttcgccgt ctatccgatt tttcctccga tctggcgcac gagttgcgca
     1741 caccggtcag caacctgctg acgcaaacgc aggtgaccct gtccaaaacc cggacgcgtg
     1801 aagagtatca ggacgtgttg gcgtccaatg cggaagagtt cgagcggctg acgaggatga
     1861 tttcggacat gctctttctg gccaaatcgg ataacagcct gatcgttccg catcgcgagc
     1921 gcgtcgacct ggcggccgag atcggaagtc tgtttgagtt ctatgaggcc ctggtcgaag
     1981 aaaagggggt gactctgagt cgttccgggg agggttcggt tttcggcgac cggttgatgc
     2041 tgcgtcgggc cgtcaataat ctgctatcga acgccgtgcg ccatacgccg aaaggcgggg
     2101 tgatcaaggc agtaattgac gattccgatg ctgcgatcat caccctgtca atcgaaaaca
     2161 ccggggaaac catcgctccc gagcacttgg tgcgattgtt cgatcgcttc taccgagcca
     2221 atgcatcccc gcagagtttc ggtgaaggcg cagggcttgg attggccatt acgcgctcaa
     2281 tcgcgcacgc tcacggcgga gaggttcttg cgcattcgga aaacggagtg acgcgcctag
     2341 agattcggct tccggtctag gtgcaggttc tcacctgctt gcctgttcag ctcctttgcc
     2401 gtcgatcaac ggagtaacgc cgaccagctc cgaggaaacg cctgaaccaa cccccttggc
     2461 gaccgatcag gctatttggg agaggcgctc gcaacaaaat cgcctagaat ggcgatgctt
     2521 ttgccatttc tcgattgatg ccacaacgct gcaacaaaca ggcaggaaca catgaaaaac
     2581 cgaccgaatt attccacgct cgctgtcttg gcgaccggcg gcatgttagc cgccggctgt
     2641 gtcgtcgttc ccggcgttga ggttaccgcc ttgccgacgc gcatcgacta tgcctgcgcc
     2701 ggcggcaaga ccctaccggt cgcgcgcgcg ccggaacagc gcatggcggc ggtgctggtc
     2761 aacaaccggg aaatcctctt gcgcgccgga gaaagcgccg cgcaggagaa atacaccgac
     2821 ggccagtact cgctttatct ggacggtgaa aaggcttttc tcgaagagaa cggcatcgtt
     2881 ctcttcggcc cctgtacttc gcctgtgccc ttgtccaagt attaccgcac gtactagcag
     2941 cgcgctatca ataccatttc aaactcgccc attgcgctac ttccggtgcg ggaagagctt
     3001 ggcttggggt tgccgtaaat ttcgcaaatg gtcgtaatcc aagtgtatgt catgttccac
     3061 aagggtctct tgacgaccct gaacggacct tcagcctctt gagatgcgga cagtcgacgt
     3121 gaaactcagc cgatggcaac agagttcacc attttgacgg ttggcgggag cggatcgttc
     3181 cgttgttcat ttgtatgcga tatagaacgg gacgccaacg gcgcagagca tgaccaatga
     3241 cgtgtacagg ctttgccgaa agtgtgggcc gcagaatctg acatagacaa caaacaacaa
     3301 aattggccac aaacagaaca acgtggtgtt gatggatgcg cgcgcaggat tcaacgccac
     3361 catcgacggc gaaaaccatg caaggactgc gtagagtagc gagatcacac tgccgcagac
     3421 gacggtcaga ccctcaaacg tatgtagcac cggcccgcga aaaagggcgg aaagagattc
     3481 accgtaccac ccaaggccac tcgcagccct aagtgcgggg tcttgttcgc gcttagcggc
     3541 ggctcgtagt gcgtgccaaa aaaacgcaag aattcccaag aacccgacca tgacgaaaac
     3601 gccataccag tcatgctttg tgaaagcgac aaagatgccg aatgtggcaa gcgctagcag
     3661 gagtgtgatg ccgaggtagc cgatagcgcg aggcaggctc atgaagacct gaggctcgga
     3721 taaaggattg ggcgtcgatt taccaacgcc aatggtatct ccgcctattc tcatgatgaa
     3781 gtgcaacatc atcgcgccag tctcgggggc ttaccccttg accgaagatg ccgacccaaa
     3841 cgcaaagcgc ggccagcgcg agaaaaaaga ggatagccac tattccttcc aagaaggtta
     3901 agtgttctcc tgcgcgaaat ggatttccat agattggaaa ccaaaccgct ccaacgagga
     3961 aaagcaccgc acctagcaat ctaggcgcga gtagccatag cggaaat
//
LOCUS       DAORPM010000225         4003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7065_length_4003_cov_16.423759, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000225 DAORPM010000000
VERSION     DAORPM010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7065_length_4003_cov_16.423759"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1492)
                     /locus_tag="PLD53_10025"
     CDS             complement(134..1492)
                     /locus_tag="PLD53_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HRC61295.1"
                     /translation="MTPTLENIDLLIEARWIATVDTDLVLKNHAVAVNNGRILAVLPT
                     SEARSRFLAAERVTLDEHILIPGLVNLHTHAAMTLLRGIADDLPLREWLQEHIWPAEA
                     EHISSKFVYDGTRLACAEMLRGGITCFNDMYFYPEAAAAAAAEYGMRAMIGITAIEFP
                     TPYASDADDYINKGLAIREMWLDNPLIGFCLAPHAPYTVSDITFERVLTLSEQLDLPI
                     HCHIHETRHELEESQQTYQESPIERLNGLGLLGPGFIGVHAVHLTESELELIAATGSS
                     IAHCPTSNLKLASGFAPVARMRQLGINVGLGTDGAASNNRLDLFSEMRLAALIAKGVT
                     GDAAVLPAHEVLRMATLNGAIALGLGNEIGSITPGKAADLCAVHLGSLETKPCFDPVS
                     HLVHVAGRESVSHVWVAGKCCVDNKSLANSAQNDLDAAIALWQNKLEFRPNTSSDVLR
                     AD"
     gene            1591..>4003
                     /gene="gyrA"
                     /locus_tag="PLD53_10030"
     CDS             1591..>4003
                     /gene="gyrA"
                     /locus_tag="PLD53_10030"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRC61296.1"
                     /translation="MDQFAKETLPISLEDEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLFAMHELSNDWNKAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQNFSLRYML
                     VDGQGNFGSVDGDSAAAMRYTEVRMAKIGHQMLEDLDKETVDFGPNYDGSEHEPLVMP
                     ARIPNLLINGSSGIAVGMATNIPPHNLNEVVAGCLALLENPALTIDDLIEYIPAPDFP
                     TAALIYGVMGVREGYRTGRGRVIMRGRTHIEDMEKSNNRQAIIIDEIPYQVNKKTLIE
                     KVAELVNEKKIEGISDIRDESDKSGMRVVIELKRGEVAEVILNNLYKQTQLQDTFGMN
                     MVALVDGQPRLLNLKQMLECFLSHRREVVTRRTVFELRKARERGHILEGLAVALSNVD
                     DIIALIKAAPTPADAKRGLMERLWRSAMVEEMLVRAASDASRPDGLAPEFGLSEQGYR
                     LSDAQAQAILELRLQRLTGLEQDKIVSEYKDVMAKILDLLDILAKPERITEIIVGELT
                     VIRDQFGDERRSEIVLHTQELGIEDFITPQDMVVTLSHGGYIKAQPLTDYRAQRRGGR
                     GKQAAAIKDEDFVDQLFVANTHDYILCFSNRGRCYWLKVYEAPQGSRVSRGKPIVNLF
                     PLEEGERINAVLPVKEFSDDQYVFMATVMGTVKKTPLSDYSNPRKAGIIAVALDEGDY
                     LVGVELTNGQSDIVLVSNAGKAVWFDEEDVRPMGRGARGVRGMRLQPGQSVISLLVAE
                     SDQQTVLVATENGYGKRTVLADFRHSGRGTQGVRAIADSERNGTVVGAKLVNDEDEIM
                     LITTGG"
BASE COUNT          844 a         1061 c         1217 g          881 t
ORIGIN      
        1 taacgtaaac gcgctcttgc gcctgggcaa cggtggcacc gaagccgata ccggccagta
       61 gtgccagagc cagtgatttc tttgcaatat ttttgatcat ttttttccct cgttgaagaa
      121 aatctgaaga cttttaatcc gctcgaagaa catcggaaga agtatttggg cgaaactcca
      181 acttattttg ccatagggct atcgctgcat ccaagtcatt ttgcgcggaa ttcgccaagc
      241 ttttgttgtc tacgcaacac tttccggcaa cccatacgtg gcttaccgat tcacgtccgg
      301 ccacatgcac caaatgtgac acgggatcga agcagggctt tgtttccagg ctgcctagat
      361 gaactgcgca aaggtcagca gccttaccag gggttatcga gccgatttca ttgccgagac
      421 caagggcgat ggcgccgttc agcgtggcca tacgcagcac ttcatgggca ggcagcacgg
      481 ctgcgtcacc ggttacccct tttgcgatca gtgcggcgag acgcatttcg ctgaaaaggt
      541 cgaggcggtt attgctggcg gcgccgtcgg tgccgaggcc gacgttgatg ccgagttgtc
      601 gcattcttgc cacaggggca aagccgctgg ccagtttgag attggaggtg gggcagtgag
      661 cgatactgct tccagtggcc gcaatcagtt caagttcgct ttctgttaaa tgcacggcat
      721 ggacaccgat aaatcctggc ccaaggagcc caaggccgtt tagccgctca atgggtgact
      781 cttgataagt ctgttggctt tcctcaagct catgccgtgt ttcgtgaata tggcaatgga
      841 tcggcaggtc cagttgctcg gacaaggtca gtacgcgttc aaaagtgata tccgatacgg
      901 tgtagggcgc atgtggcgcc aggcagaagc cgatcagagg gttgtccagc cacatttccc
      961 ggatggcgag tcctttattt atgtaatcgt ctgcatcgct cgcgtagggc gtcgggaatt
     1021 cgatggcggt gatgccgatc atggcgcgca taccgtattc ggcagcggct gcggcggcag
     1081 cttccgggta gaaatacatg tcattaaaac aggtgatgcc ccctcgaagc atctcggcgc
     1141 aggcgagccg ggtgccgtca taaacaaact tggacgagat gtgctctgcc tccgccggcc
     1201 aaatatgttc ttgtagccat tcccgaagtg gcaggtcgtc ggcgatgccg cgcagcaggg
     1261 tcatggcagc gtgggtatgg aggttgacca ggccgggaat caggatgtgc tcatcgagtg
     1321 tcacgcgttc ggctgccaag aagcggctgc gtgcttcgct ggtcggcaat acagccagaa
     1381 ttctcccgtt gttgacggcg accgcatggt tttttagcac caagtcggtg tcgaccgtag
     1441 caatccagcg cgcttcgatc aggaggtcga tattttcgag tgttggtgtc atggaaaacg
     1501 ggttcggctc aatgttggtg cgtgttaaaa tagcaaatta cgctcagttc aaccaataaa
     1561 gtcatcgcgc aagcggtgcg aatgtgagac atggaccaat tcgccaaaga aacactgccg
     1621 atcagcctcg aagacgagat gcggcgttca tatctggatt acgcgatgag cgtgatcgtt
     1681 ggccgggcgc tgcccgatgc gcgcgacggc ctgaaaccgg tgcaccgtcg cgttttattt
     1741 gcgatgcacg agctgagcaa cgactggaat aaagcgtaca agaaatcggc ccgtattgtc
     1801 ggtgacgtga tcggtaagta ccacccacac ggcgatactg cggtttatga cacgatcgtc
     1861 cgtatggcgc agaatttttc gctgcgttac atgctggtgg atggtcaggg taacttcggt
     1921 tcggtcgacg gcgatagcgc ggcggcgatg cgttacaccg aagttcgcat ggccaagatc
     1981 ggccaccaga tgctggaaga cctcgacaag gaaacggttg attttggacc gaactacgat
     2041 ggttccgagc atgagccgct ggtcatgccg gcgcgcattc ccaatctgct gatcaacggt
     2101 tcttctggta tcgcggtcgg catggcgacg aatattccgc cgcataacct gaatgaggtt
     2161 gtcgctggct gtctggcgct gctggaaaac ccggcgctga cgattgatga tctgatcgaa
     2221 tatattccag ctcccgactt cccgaccgct gcgctgatct acggcgtaat gggtgtgcgc
     2281 gaaggctacc ggactggccg cggtcgcgtc atcatgcgcg gtcgtaccca tatcgaggat
     2341 atggaaaaga gcaacaatcg tcaggccatc atcattgacg agattcctta ccaggttaac
     2401 aagaagacct tgatcgaaaa ggtcgctgag ctggttaacg agaagaagat tgaaggtatt
     2461 tccgatatcc gcgatgagtc ggacaagtcc ggcatgcgcg ttgtcattga gctgaaacgc
     2521 ggcgaagtgg ccgaggtgat cctcaataat ctgtacaagc agacgcagtt gcaggacacc
     2581 ttcggcatga acatggtggc gctggttgat ggccagccgc gcctgttgaa cctgaagcag
     2641 atgctcgaat gcttcctgtc gcatcgtcgg gaagtcgtta cacgccgtac cgtatttgaa
     2701 ttgcgcaagg cacgtgagcg tggccatatc ctggaaggtc tggctgtcgc gctgtccaat
     2761 gttgatgaca tcatcgcgct gatcaaggca gcgccgacgc cagccgatgc caagcgcggg
     2821 ttgatggagc gcctgtggcg cagcgcgatg gttgaggaaa tgctggtgcg ggcggcttcc
     2881 gatgcctcgc gtccggatgg cctggctccc gagtttggtc tgtctgagca gggttatcgt
     2941 ctctccgacg cccaggcgca ggcgattctc gaactgcgcc tgcagcgcct gactggcctt
     3001 gagcaggaca agatcgtcag cgagtacaag gatgtcatgg ccaagattct cgacttgctc
     3061 gacattcttg ccaagccgga acgcattacc gaaatcattg ttggcgaact gacggtcatt
     3121 cgtgatcagt ttggcgacga acgccgttct gaaattgtgc tgcacactca ggaactcggc
     3181 atcgaagact tcatcacgcc gcaggatatg gtggtgacgc tttcgcacgg tggctacatc
     3241 aaggcgcagc ctttgaccga ttaccgcgcc cagcgccgcg gcggtcgggg caagcaggca
     3301 gcagcgatca aggacgagga tttcgtcgac cagctgtttg tcgccaatac gcatgactac
     3361 attctgtgct tctcgaaccg tggtcgctgc tactggttga aggtttatga ggcgccgcag
     3421 ggtagccgcg tcagtcgcgg caagccgatc gtcaatctct tcccgctgga agagggcgag
     3481 cggatcaacg ccgtgctgcc ggtcaaggaa ttctccgacg atcaatatgt cttcatggcg
     3541 accgtcatgg gcaccgtcaa gaagacgccg ctgtccgatt actccaaccc gcgcaaggcc
     3601 ggcatcatcg ccgtggcgct ggatgagggt gattacctgg ttggcgtcga gctgaccaat
     3661 ggccagagcg acattgtgct cgtctcgaat gccggcaagg ccgtctggtt cgacgaagag
     3721 gatgtccgcc cgatgggccg cggcgcccgc ggtgtgcgcg gcatgcgctt gcagccaggg
     3781 cagtcagtga tctccctgct cgtcgccgag agcgatcagc agaccgtgtt ggtggcgacc
     3841 gagaatggct acggtaaacg cactgttttg gccgatttcc gccactccgg tcggggcact
     3901 cagggcgtcc gcgccattgc cgacagcgag cgtaacggca ctgtggtcgg tgccaagctg
     3961 gtcaatgacg aagacgaaat catgttgatc accaccggtg gtg
//
LOCUS       DAORPM010000226         4000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7075_length_4000_cov_7.424081, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000226 DAORPM010000000
VERSION     DAORPM010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7075_length_4000_cov_7.424081"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..830
                     /locus_tag="PLD53_10035"
                     /pseudo
     CDS             1..830
                     /locus_tag="PLD53_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            1149..2036
                     /locus_tag="PLD53_10040"
     CDS             1149..2036
                     /locus_tag="PLD53_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRC61297.1"
                     /translation="MNMREEQSPGLAEDKRGEALNAERFRMLEDIARELAGEVVFPTY
                     FDAALRLRKDLQNPDLPTARIAKIVSIEPLIAAKLMQLAGSALYSPDGSPAKDLSAAI
                     SRLGVDLVRTTALAIAMNQLLRSKDMAIFSDLTRALWDHSIRTAAAARVLAQNFTRIN
                     PDEALLAGLVHDLGAFYMLYRAVQYPELRVRPDSIKYLIVQWHEGIGVTLLDTLGMPK
                     EIVNATIDHDQPRLAPITVRTLADIVYVGNLIAGAHFEWSHQDFDPDAGQVGQVRQNF
                     SALLPEIEADTREMLAVFS"
     gene            complement(2108..3331)
                     /locus_tag="PLD53_10045"
     CDS             complement(2108..3331)
                     /locus_tag="PLD53_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRC61298.1"
                     /translation="MIVRATFLSLIASVLLFVQGCATPPRLPAVPSELTARAEIPGMP
                     SVRFALGGDMTELTQIAHASLQRERDYLAKQGHKGPFPPAFFLAISGGGDNGAFTAGL
                     INAWTATGTRPEFKVVTGISTGALIAPFAFLGPKYDATLKEVYTTISPKDILEPRSFI
                     GGVMSDGMADNAPLLKLTRKSVTEDFLKEVAAEYAKGRFLLIATADLDARRPIIWDMG
                     KIATYGGPKALDLFVRVMIASASVPGGFPPMMLDVEVDGKAYQEMHVDGGIMAQVFAY
                     PAALRINESAAAVGVTRERKLYVIRNARLDPDWAQVERSTMSIAGRSVASLIHSQGIG
                     DLYRIYATAQRDGVDFNLGFIPSSFKAEHKEEFDTDYMRALYKTGYDMTLKGFPWLKV
                     PPGFAPPTAVPAAIR"
     gene            3757..>4000
                     /locus_tag="PLD53_10050"
     CDS             3757..>4000
                     /locus_tag="PLD53_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61299.1"
                     /translation="MKLTIGNPVVGDDFFDRELEQRQIWRKLESNHLLMLAPRRIGKT
                     SLIYRLCQDSQTHRFFSLHCSFAGCEDERNCVQELFK"
BASE COUNT          870 a         1155 c         1150 g          825 t
ORIGIN      
        1 tcaagggcag catcgtgctc aagaaagcct actgcgactg ggatcgctac aagggcttca
       61 aggcgacgat gcacgaggcc aatttcgaac tgatcgaaat cccgcatgtc cggcagtcgg
      121 gcaagaattc cgctgatatc cgtatggtgg tcgatgcgct cgacttgtgt tacacgaagt
      181 cgcacgtcaa ctgcttcgtt atcatcagcg gcgactcgga tttctcgccg ctcgtttcca
      241 agctgcgcga gaacgccaag tacgtgatcg gcgttggcgt caagcaatcg acttccgacc
      301 tgctgattgc caactgtgac gaattcatct tctacgacga tctggtgcgt gaaatccagc
      361 gttcggccgc gcgccgcgac ccgaaggcag tgcagcttgc atccaaacgc acgcctgaag
      421 aaaacgagaa acgtaagcag gaaatcgaag cgcgcaagag tgaggcagtc gagctggccg
      481 tggcgacgtt cgatgcactg gtttcagaac gtggtgatgg cggcaagatc tgggcttcga
      541 tgctcaagga ggcgatcaag cgccgcaaac ccgatttcaa cgagagttac tacggcttcc
      601 gtgctttcgg caatctactc gaagaagcac agtcacgcgg gctgctcgaa ttgggacgcg
      661 acgaaaaatc cggagcgttc gtgtcgcgca gtagcgcacc ggcaccgcgt agcgaaccgg
      721 tcgtggcggc tgtagaggag tccagcgccg cccccgcgcc aaagcgccga gggcgaggcg
      781 gcagtagaaa accagcgtcg gacgtcgcgt cggagcctgc cgttcaggaa atacctgtcc
      841 cggctgagcc tgcaatcgcc caggccgagg aagagattcc cgcaaaaaaa ccggcctctc
      901 gcagccgccg gccacgcaag ccgaaagcca cgcccgacga tggcgagcca acagcctgag
      961 ttccaagcga caggttcggg ctgaatagtg cggctggtag atgagtgaag gctttttgcg
     1021 atggacgaaa atgttaagat tgcgccttcg acagaaggtg accaggcgcg agactggtcg
     1081 accggtttgc ccccattgag ggcactgcca gcggtcaatc tggaacaagg aagagtcgtc
     1141 agagagtcat gaatatgcgc gaagaacaga gcccgggact ggcagaagac aaacgtggcg
     1201 aagccctcaa cgccgaacgc ttccggatgc ttgaggatat cgcccgggaa ctggcaggcg
     1261 aagtggtctt ccccacctac ttcgacgccg ctttgcgtct gcgcaaggat cttcagaatc
     1321 cggatttgcc tacggcgcgc attgccaaaa ttgtcagtat cgaacccctg atcgccgcca
     1381 agctgatgca gctcgcgggt tcggccctgt atagcccgga cggttcgccg gccaaggatt
     1441 tgagcgccgc gatcagccgg ctgggcgttg atctggtgcg taccactgcc ttggcgatcg
     1501 ctatgaacca gctcctgcgc tccaaggata tggcaatctt cagcgacctg acccgcgccc
     1561 tctgggacca ttccatccga acggccgcgg ctgcccgtgt cctcgcccag aatttcaccc
     1621 ggatcaatcc ggacgaagcg ctactggccg gcctggtgca tgacctcggt gccttctaca
     1681 tgctctaccg cgcggtccag tatccggaat tgcgcgttcg tccggatagc atcaaatacc
     1741 tcatcgttca gtggcacgaa ggcattggag tcaccttgct cgacaccctc ggtatgccca
     1801 aggaaatcgt caacgccacc attgaccacg accagccacg gctcgcaccg ataacggtac
     1861 ggaccctggc ggatatcgtc tatgtcggca acctgattgc cggcgcccat ttcgaatggt
     1921 cgcaccagga tttcgacccg gatgccggtc aagtcgggca ggtccgccag aacttcagcg
     1981 cactgctgcc ggaaatcgag gccgacacgc gggaaatgct ggccgtattc tcctgaatct
     2041 ggtggctttc cgcgtgctgt gacgccggca tcagaagatg cgcatgaaat tcgtacgcaa
     2101 cgttcactca cctgattgcc gctggcactg ctgtcggtgg agcaaatccc gggggaacct
     2161 taagccacgg gaagcccttt agggtcatgt cgtaaccggt cttgtacagc gctcgcatgt
     2221 aatcggtgtc gaattcttcc ttgtgctccg ccttgaagct cgatgggatg aacccaaggt
     2281 tgaagtcaac tccatctcgc tgggccgtgg catagatgcg gtaaaggtcc ccgattcctt
     2341 ggctgtgaat cagcgaggca accgagcgac cggcaatgct catggtactg cgctcaactt
     2401 gcgcccagtc cggatccagt cgcgcattgc gtatgacata aagtttgcgc tcacgggtaa
     2461 cgccgacggc agcggccgat tcgttgatgc ggagtgcggc cgggtaagcg aaaacctgag
     2521 ccatgatgcc gccatccacg tgcatttcct gataggcctt tccatcgact tccacatcaa
     2581 gcatcatcgg aggaaaacca ccgggaaccg aggccgatgc gatcatgacg cgcacgaaca
     2641 ggtccaaggc cttggggcca ccgtaggtcg cgatttttcc catgtcccaa atgatcggac
     2701 gacgcgcatc gaggtcggcg gtggcgatca agaggaaacg tcctttcgca tactctgcgg
     2761 ctacttcctt gagaaaatcc tcggtcaccg attttcgcgt cagcttcaac agcggtgcat
     2821 tgtcggccat tccatcgctc atcacgccgc caatgaaact gcgcggctca agaatgtctt
     2881 tcggagaaat ggtggtatag acctctttca gcgttgcgtc atacttcggc ccgagaaacg
     2941 cgaaaggcgc aatcagggcg cccgtactga taccggtgac cactttgaat tccgggcgtg
     3001 tccctgtagc cgtccatgca ttgattagcc cggcggtgaa ggcgccgtta tcaccaccgc
     3061 cagaaatcgc caaaaagaag gctgggggaa acggaccttt gtgcccctgt tttgcaaggt
     3121 aatccctttc ccgttgcagc gacgcgtgcg caatctgggt gagctcagtc atgtcgcccc
     3181 ccaaggcaaa gcgaacactt ggcatgccag gaatttcggc tctcgccgtc aactcggatg
     3241 gcactgccgg caagcgtggc ggcgtcgcac agccctggac aaacagcaat acgctggcaa
     3301 tcaggctaag gaaggtggct cgtacaatca tttgtctgct tcctgagtaa gtattcgagg
     3361 aaccgtctcc cctcggggtg ttccctgcag tcctgttcga ggtgggtatt atggactgcc
     3421 tcgaaagcag tacgcaaatc gcagctacgc ctcgaggttg attcaatggc tttggggttt
     3481 gcagcggctg gatcgtacgt ggagcccagt cttctgcttc cagcaagtag gatcgcctcg
     3541 cgcacttcac tgcggttatt ggccttggtc aagtcgtttg ccgcgtgtca acgcaccaga
     3601 gtgctttcag agtcatcgca cgggcttctc aatggtcggc tgatgccaag cgtcgaaggt
     3661 ccgcgttccg ttcggctgta cgtaaaaccg ctttacaaca aacccgctgt acatctaaaa
     3721 tgttgtacat caatattgtt gtatatgagg cttggcatga aattgactat aggaaatccc
     3781 gtcgtaggtg atgacttctt cgaccgtgaa ctggaacagc gccagatatg gcgcaagctt
     3841 gagagtaatc acttattgat gctcgcgccg cggcgcatcg gcaagacatc gctgatctac
     3901 cgtctttgcc aggactcgca gacgcatcgt tttttcagtc tgcattgcag ctttgccggt
     3961 tgcgaagacg agcgtaactg cgtacaggag cttttcaagg
//
LOCUS       DAORPM010000227         3988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7097_length_3988_cov_12.250445, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000227 DAORPM010000000
VERSION     DAORPM010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7097_length_3988_cov_12.250445"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLD53_10055"
     CDS             complement(<1..231)
                     /locus_tag="PLD53_10055"
                     /inference="COORDINATES: protein motif:HMM:NF015678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HRC61300.1"
                     /translation="MSTLGNILWFIMGGVLMGLGWWLVGLLAFISIIGIPWGKACFVI
                     GQFAFLPFGKEAVNRRDLSRQDDIGTGFFGLLG"
     gene            complement(330..1649)
                     /locus_tag="PLD53_10060"
     CDS             complement(330..1649)
                     /locus_tag="PLD53_10060"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HRC61301.1"
                     /translation="MLQSMVSKQTVCLRQLAGSRAREVQYGRWLANDKVTPAQLVVSA
                     CARTGAIAAGRRHVLAIQDTTELNHQAHAGRVSGLGTVGNGSDVGMFLHPLLVVDARD
                     GACLGLAHLHHWIRTGKASPDYRKYPIEDRESYRWLDVAEAGKKCLSEAAMVTIIADR
                     ESDIYEEWARVPDERTHLITRACRDRTLAAGDKLYAWIDAQPAQGTHRFDVPARPGTR
                     SAHRATLDVRFGRVTIRRPASCSDKSAPPQIELSVVEVKELPGSVVGNEAPIHWRLLT
                     THRIACLEEALQCVDWYRQRWHIEQLFRTLKKQGLNLESSQVETAAGLTKLACLAIQA
                     AVLTMQLTLARDGHSAVQAADVFNADEIEVLQRIRPTLEGKTQKQKNPHRTGSLAQAA
                     WVIARLGGWKGYASEAKPGPITMLHGLQRLSAICQGRKLATKDVCID"
     gene            complement(1902..3521)
                     /locus_tag="PLD53_10065"
     CDS             complement(1902..3521)
                     /locus_tag="PLD53_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61302.1"
                     /translation="MELFTKLFGRLLVFMYHCFDRIVIHGYLSGLSRPEQVVYFFRQV
                     VGIDTVDKDVLSRRTNEYQQWVEAFARNHETPVEWAEKGVRKEDHVLPWQRAMVRAKR
                     YGVYFIFKSMEQGPTFRCTTPKYPTKDPNHRILAPQRSRFTHYYFYIRDESLGPLVMR
                     VASFFPFQTTYYLNGHSFIEQELNRAGIGFRKNDNAFLAVDDPLALQAAADRLNPELI
                     RERLDYWTLILGPKFSAKERQRINLRRFYAIAQIEYCRNFIFKRNFPIHKIFERSCEL
                     GLWSLSANKISEIFGGRITQRLQGKLQTTLEQIEHGHHIFRAYWKHAFIKQYEKFATY
                     LRNEICSNNLSDFGLKKGLEHIAAVREKFLAITERFASFQAIGLNAHVDFPLLQRLAL
                     PITVGSVRYPGIKIHEVRMIRLMQVLLHASTTVGGWRAGQLHEALLMSFGLSASRYGL
                     NQLRYDLRKMKGHGLLERDGTHYAYRLTDKGVKVALLFVLFHQRLCGPLANSLFHHRP
                     DANFQPDSKLEAALHKADTSIHNVIQLIEAA"
     gene            3596..>3988
                     /locus_tag="PLD53_10070"
     CDS             3596..>3988
                     /locus_tag="PLD53_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61303.1"
                     /translation="MTNRKTRHRIATQGLSRERFHLTAEIRLIQHRAAEHEGRIVGLG
                     SLLLFSTDTGDAWILDPADQLAARLARDGDPLAVYVEESESKYAIGWQGHFRIDGDLF
                     EYEDNDALHKVTIHGYPTSLLLRRIEKLD"
BASE COUNT          870 a         1142 c         1104 g          872 t
ORIGIN      
        1 gccgagcagg ccgaaaaagc cggtgccgat gtcatcctgc ctggacagat cccttcggtt
       61 caccgcttcc ttgccgaaag gaagaaaggc gaactgcccg atcacgaaac aggccttgcc
      121 ccacggtata ccgatgatcg agatgaaggc caacaggccg accagccacc agcccagccc
      181 catcagcact ccccccataa tgaaccacag aatattgccc agcgtactca tttgaagtct
      241 ccgttcatga acgcgattaa agctgaaaag agtagtttag catgatgcca atagcgtaag
      301 ccctcatccc cagcccttct cccgtggggc taatctatgc acacatcttt tgtagccaat
      361 ttcctgcctt ggcagatcgc cgagaggcgc tgcaatccat gaagcatggt gatagggccg
      421 ggcttggctt cggaggcata tcctttccat ccgcccagac gcgcaatgac ccatgcggct
      481 tgggcaaggg agcctgtgcg gtgagggttt ttctgctttt gcgtttttcc ttccagagtc
      541 ggccgtatgc gctgcaagac ttcgatctcg tccgcgttga atacgtcagc ggcttgaacg
      601 gcgctgtgtc cgtcgcgggc caaggtcaat tgcatggtga gcaccgccgc ctgaatagcc
      661 agacaggcca gcttggtcag tcccgccgcg gtttcaactt ggctggactc aaggttcaag
      721 ccttgcttct tcaaggttcg gaacagttgt tcgatgtgcc agcgctgccg gtaccagtcg
      781 acgcattgca aggcctcctc aagacatgcg atacggtgcg tggtcagcag gcgccagtgg
      841 atgggcgctt cattgcccac cacgctgcct ggcaattctt tgacttcaac aacgctcaat
      901 tcaatctgcg gcggtgcgct tttgtccgag caggaggcgg ggcgccgaat cgtgacacgc
      961 ccaaagcgta cgtccagcgt ggctcggtgg gcgctacgcg tgcccgggcg ggccggtacg
     1021 tcaaagcggt gggtgccttg tgcgggttgc gcatcgatcc acgcatacag tttatcgccg
     1081 gcggccaggg tgcggtcacg gcaggcacgc gtgatcagat gcgtgcgctc gtcaggcacc
     1141 ctcgcccatt cttcatagat atcgctttcc cggtcggcaa tgatcgtcac catggccgct
     1201 tcggacaggc attttttgcc ggcttcggcg acgtccagcc agcggtagga ctccctgtct
     1261 tctatcgggt atttccggta atcgggggag gctttgcccg tcctgatcca gtgatgaagg
     1321 tgcgccaagc caagacacgc tccatcacgt gcatcaacaa ccaacaaggg gtggagaaac
     1381 atgcccacgt cgcttccatt ccctaccgtg cccaggccgc tgacgcgccc tgcgtgggct
     1441 tggtggttca gctctgtcgt gtcctggatc gccagcacat gccgccgccc cgcggcaatc
     1501 gctccggttc gcgcacaggc actgacgacc aactgcgcag gcgtcacttt gtcattcgcc
     1561 agccagcgac catactgcac ctctcgcgcc cggcttcctg ccagttgccg aagacagacc
     1621 gtctgcttcg acaccatgct ctgtaacaag gccgctccgt tttttttaac cgaacatcgc
     1681 caaaatcacc cacatcgtag gtctcattcc tctccaacgc ttgcacactt tccatgtcaa
     1741 tcctccggtt atcattcacc aaaagattca aagcaaaatg tgtgcataga ttagccctgc
     1801 gggagaaggg ctggggatga gggcctacga aattggcatt acgcgcattt gcgttcttta
     1861 gatacttacg attttaatgt ttcacaaaac gtgttcaaca attatgcagc ttcgatgagt
     1921 tgaattacat tgtgaatcga ggtatctgcc ttgtgtaaag ccgcctcgag tttgctgtcg
     1981 ggctggaagt tggcgtcggg ccggtgatga aacaaactgt tggccagcgg tccacacagg
     2041 cgctggtgga agagtacgaa aagcagcgca actttcacgc ccttgtcggt gaggcgataa
     2101 gcgtaatgag ttccgtcacg ttcgagtaga ccatgcccct tcattttgcg caagtcatag
     2161 cgcaactggt taagcccgta gcgtgacgcc gagaggccga agctcatcag caaggcctca
     2221 tggagttgcc cggcacgcca accgcctacc gtggtactcg catgcaacag cacctgcatg
     2281 agtcggatca tgcgaacctc gtgaatcttg atgcccggat agcgaacgct acccaccgta
     2341 attggcaggg cgagccgttg cagcaagggg aagtcgacgt gggcattgag gccgatggcc
     2401 tgaaacgagg caaagcgctc ggtgatggca aggaatttct cgcgcacggc cgcgatatgt
     2461 tcaagcccct tcttcaggcc aaagtcggag aggttgtttg agcaaatctc attgcgcaaa
     2521 tacgtggcga acttctcgta ttgcttgata aaggcatgct tccaataggc acgaaagatg
     2581 tggtgaccgt gctcgatctg ttcgagcgtg gtctgaagct taccctgtag tcgctgggtg
     2641 atacggccac cgaagatttc ggagatcttg ttggccgata gactccacag ccccaattca
     2701 cagctacgtt cgaagatctt gtggatcggg aaattccgtt tgaaaatgaa atttcggcaa
     2761 tattcgatct gggcgatggc atagaaacga cgaagattga tccgctggcg ctccttggcc
     2821 gagaacttgg gccccaggat caacgtccag tagtcgagcc gctcgcgaat cagttcggga
     2881 ttgagccggt cggcggccgc ttgcagggcc agcggatcgt ctaccgccag gaaggcattg
     2941 tcgttcttgc ggaagccgat gcccgcccgg ttcaattcct gctcgataaa cgagtggcca
     3001 ttgaggtaat aggtcgtctg gaatgggaag aacgaggcca cgcgcatcac gagggggcca
     3061 agcgattcgt cgcgaatgta gaaatagtaa tgcgtgaatc gactgcgctg cggcgccagt
     3121 atccgatgat tcggatcctt ggtcggatac ttcggtgtcg tgcagcgaaa cgtcggccct
     3181 tgctccatgc tcttgaaaat gaaatagacg ccatagcgct ttgcccgcac catggcccgc
     3241 tgccacggta agacatgatc ttccttgcgc acccctttct cggcccactc caccggcgtc
     3301 tcgtgattgc gtgcaaaggc ttctacccac tgctgatact cattggttcg gcggctcaat
     3361 acgtccttgt caacggtgtc aatacccacc acctgtcgga agaagtaaac tacttgctcc
     3421 ggacgggata atccgctcag gtagccgtgg atgacgatac ggtcaaagca gtggtacatg
     3481 aagaccagca gacggccaaa cagtttggtg aagagttcca tcattccctc caagtcgcgc
     3541 tgccctccgt gaagcgcagc gggacatggg tgcgttcaca ggaggacaca aaccgatgac
     3601 taatcgaaag actcgccaca gaatcgccac gcagggatta tcacgtgagc gtttccatct
     3661 taccgctgaa atccgcttga tccagcatcg tgccgccgag cacgaggggc gtatcgttgg
     3721 cctcggatca ctgctccttt tttctaccga caccggcgac gcatggattc ttgatccggc
     3781 ggatcaactc gcggcacgct tggcccgcga cggcgatcca ctcgccgttt atgtcgagga
     3841 aagcgagtca aagtatgcta tcggatggca gggtcacttc aggatagatg gggatctatt
     3901 cgagtacgaa gacaacgacg ctctacataa agtgacaatc cacggctacc caacatcact
     3961 actacttcgt aggatagaaa aactggat
//
LOCUS       DAORPM010000228         3975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7132_length_3975_cov_7.271684, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000228 DAORPM010000000
VERSION     DAORPM010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7132_length_3975_cov_7.271684"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..730)
                     /locus_tag="PLD53_10075"
     CDS             complement(227..730)
                     /locus_tag="PLD53_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61304.1"
                     /translation="MLISSGSPTRSLLTFGLLLCGLLFAAAPGWAGGQSTVSGIFKGN
                     GKDTRLAHVSAAKGEPLAGKPTILLVFTEKDHSKDDRPQIKATFGGFGSALIVTIDES
                     GDIVGCEVAHAAHEKQGFSSIGKVNLTDFKRTPGVIQGRLTTNGEVKTFGEKWELDLK
                     FNAALPK"
     gene            complement(775..1548)
                     /gene="djlA"
                     /locus_tag="PLD53_10080"
     CDS             complement(775..1548)
                     /gene="djlA"
                     /locus_tag="PLD53_10080"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HRC61305.1"
                     /translation="MFKLIFFIAGFYFSGFFGALFGLFIGSLIDRSRAYGPGGINPLQ
                     NANRQSVFLETMFISMGKLAKADGHVSQDEIAHVEQLMQKLGMTAEHRAQAIALFKRG
                     ADPAFEIGATYARFISVCGNTKHLRQILLVYLIVMATADGHIHPAEETVLADVARRLG
                     YDPATFRHLMEMVLNQSHFAAGQPKTAAALDDAYKALGVTKDNTDKEIKHAYRQLISQ
                     YHPDKLIGQGLPEDMIALATEQAKEIQLAYDLIAESRKV"
     gene            complement(1609..2505)
                     /locus_tag="PLD53_10085"
     CDS             complement(1609..2505)
                     /locus_tag="PLD53_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRC61306.1"
                     /translation="MADIFLSYSREDEARIKSLVTEFEAQGWTVFWDRRIPAGETWRS
                     YIGAALQDARCIVVAWSQSSIESQWVAEEADEGKARKILVPVLLDQVPAPRGFREIQA
                     ADISGWQPGQASERFTELIGDLRRILGKPSASAREMPPAGQPFTPERKAEGQAGGRSM
                     HGRRTVLVALLAVFAIGIAGYFVVGSLQTGKQHVPSPSIPMQAARDRAVTADATRSGG
                     SWLVVAGSFGRDDLQSAERHHLRLTRAGLDSTVIDSNDYPLLAPNLWVVAIGPFESRE
                     TANAALSRVRTTVPDAYTKMGR"
     gene            complement(2634..>3975)
                     /locus_tag="PLD53_10090"
     CDS             complement(2634..>3975)
                     /locus_tag="PLD53_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRC61307.1"
                     /translation="PDMAGRVLIHRYGPAVDSLVEELRRVGTAFVVLEQDERQARRLR
                     DRGYDVVFGALDEQPGMLSGVEAAQALITNADDHADATFIMMARERGYTGPILALAED
                     PLHRPPMEKIGATAVFTPSHVLGAALAARASARISPKVEGLQLLGERVGIADFRVQAS
                     SPLARQRLGDLRMRAHHGVTVIGQWNGGRFASAEGPDTRIEAGAILVVAGALANLARV
                     ERMATPLRLHGPIVVAGYGAVGRKVVEMLHDAGETTIVIDVRADPEVDVVGNILEHAT
                     LEHARVRNASAVVLALSNDSAGVFATAVVRDYAPEVRLIARVNLAPNVARLYQAGADF
                     ALSVGQVAGQILAHHLLGESAITVEQRLKLARVAPGTMVGRHPWKAEVREKTASAVVA
                     VERGTEVLVEFDPEFQVRADDVLFVCGTTTGLDKYMREFRATSAERPAAPAARS"
BASE COUNT          763 a         1218 c         1192 g          802 t
ORIGIN      
        1 ccggatggcc gcgttcggca ttgccggtgg cggcggcagc ggattcaagc gcctgctgat
       61 gacccatccg ccgctggaag agcgtatcgc cgcgctgaag gcatcgcagg agcgatgatc
      121 tatccgtgag acctcaggcc attcggaaaa ctgggatgca atccaggtgg agcgacgcgc
      181 ctgattgaag aggccattca gggatcatcg aaaaaattcg gggcggctac ttcggcagtg
      241 cggcgttgaa tttgaggtca agctcccact tctccccgaa ggtcttgact tctccattcg
      301 tcgtgagtct gccctgaatc acccccggcg tccgtttgaa atcagtcaaa ttgaccttgc
      361 cgatggaact gaagccctgc ttttcgtgtg cggcgtgggc gacttcacaa ccaacgatgt
      421 cgccgctttc gtcaatcgtg acgatgagtg cgctgccgaa cccgccgaag gtggccttga
      481 tttgcggccg gtcgtctttc gaatggtctt tttcggtgaa cacgagaagg atggtaggct
      541 tgccggccag cggttcgccc ttggcagcag aaacgtgggc gagcctggtg tccttgccat
      601 tacctttgaa gatgcccgat acagtggatt gtccgcccgc ccaacccggc gccgctgcaa
      661 acaacaaccc gcataggagc agcccgaaag taagcagcga tctcgtggga cttccggatg
      721 atatgagcat ggtggtgtac tcctgttgtg tgggggtcaa taggcgcaat tcactcagac
      781 cttccggctc tccgcgatca ggtcgtacgc aagctggatc tcttttgcct gttcggttgc
      841 caatgcgatc atgtcttccg gaaggccctg tccgatcagc ttgtccgggt ggtactggct
      901 gatcagttgg cgataggcat gcttgatttc cttgtctgtg ttgtctttcg tgactcccag
      961 ggctttgtag gcgtcatcca gtgcagcggc agttttgggc tgacctgcag cgaaatggga
     1021 ctggttcagc accatctcca tcaaatgcct gaacgtggcc gggtcatagc cgagacgcct
     1081 ggcgacgtcg gcgagcaccg tttcttctgc cggatgaata tgtccatccg ccgtcgccat
     1141 cacgatcaga taaaccagca gtatctgtcg gagatgcttg gtgttgccac aaaccgaaat
     1201 gaatctggcg taagtcgccc cgatttcaaa cgccgggtcg gcgccacgct tgaacaacgc
     1261 gatcgcctgc gcacggtgtt ctgcggtcat ccccagcttc tgcatcaact gttcgacatg
     1321 cgcgatttcg tcttgcgaga cgtgcccatc cgccttggcc agtttgccca tcgagatgaa
     1381 catcgtttcg aggaatacgc tctgacgatt tgcattctgc agcgggttta ttccgccggg
     1441 accataggct cgcgagcggt cgatcaatga accgataaac aggccaaaga gcgctccaaa
     1501 aaatccggaa aagtagaaac cagcgatgaa gaaaattaac ttgaacaagg tgactccaga
     1561 aagttttcgg gttaaagacg gggctgctta cccccgattc gaaaggattt agcgtcccat
     1621 cttggtgtac gcatcgggta cggtcgtcct gacgcggctc agagcggcgt ttgccgtctc
     1681 tcttgattca aacgggccaa tcgccacgac ccacaggttt ggagcaagca aagggtagtc
     1741 attcgagtcg atcacggtcg aatcaagccc cgcacgggtg agcctgaggt gatgtcgttc
     1801 ggcagactgc aggtcgtcgc ggccgaacga gccggcaacg acgagccagg aaccgcctga
     1861 gcgggttgcg tcagccgtca cggcgcgatc acgtgctgct tgcatcggaa tcgacggcga
     1921 ggggacgtgc tgtttgccgg tctgtagcga cccgacgaca aaatagcccg caatgccgat
     1981 ggcaaagacg gcaaggagtg caactaaaac ggttcgccgg ccatgcatcg atcgacctcc
     2041 ggcttgacct tcagctttgc gttccggcgt aaagggctgg ccggctggtg gcatctcacg
     2101 cgctgatgcg gacggcttgc ccaggattcg ccggagatcc ccgatcaact cggtgaagcg
     2161 ttcggaagct tgtcccggtt gccagcccga tatgtcagcg gcctggattt cacgaaaccc
     2221 gcgcggagcg ggcacctgat cgagcagcac cggaaccagt atcttgcgcg ccttcccctc
     2281 gtcggcctcc tccgcaaccc attgcgattc aatcgaagac tgcgaccagg cgaccacgat
     2341 gcagcgggca tcctgcaggg cggcgccgat atagctccgc caggtctcgc cggcggggat
     2401 gcggcgatcc cagaagaccg tccagccctg tgcctcgaat tcagtaacaa ggctcttgat
     2461 tctcgcctcg tcttctcggg agtagctcag aaaaatgtct gccattgctg tttcgagtct
     2521 tccaacaaaa cctacctgga atcagaacaa gttgtatgtc atttctggcg cacaatcgac
     2581 acctgcccgg tagatttgca acatacgcca acgcccattt taagcgatag ccgtcacgac
     2641 cgggccgctg gcgccgcggg tcgttctgcc gaggtggctc gaaattcccg catatattta
     2701 tccaggcccg tggttgtgcc gcacacaaac aggacgtcat ctgcgcgcac ctgaaattcc
     2761 gggtcgaact ccaccagtac ttccgtgccg cgttccaccg ccaccacggc ggatgcggtt
     2821 ttctcgcgga cttcggcctt ccatgggtgt ctgccgacca ttgtgcctgg cgccacgcgg
     2881 gcgagtttga gccgctgttc cacggttatc gcgctctcgc ccagcaaatg gtgggcgaga
     2941 atctggccgg cgacctggcc caccgacagg gcgaagtcgg caccggcctg gtagagtcgg
     3001 gccacattcg gagccagatt cacgcgcgcg atcagtcgca cttcgggcgc atagtcgcgc
     3061 actaccgcgg tggcgaacac cccggcgctg tcgttgctga gcgccagtac tacggcgctc
     3121 gcattgcgca ccctggcgtg ttccagggtg gcgtgctcaa ggatgttgcc aacaacatcg
     3181 acctcagggt cggcgcgcac gtcgatcacg atggtcgttt caccggcatc gtgcagcatc
     3241 tccaccacct tgcgtccgac cgccccgtag cccgccacga caattgggcc gtggagacgc
     3301 agcggggtgg ccatgcgttc gactctggcc agattggcga gtgctccggc gacgaccagg
     3361 attgcaccag cctcgatacg cgtatccggg ccttcagccg aggcgaaacg accgccattc
     3421 cattgcccga tcaccgtaac gccgtggtgt gcgcgcatgc gcaggtcgcc gagacgctgc
     3481 ctggccagcg ggctgctggc ctgaacccgg aagtcggcga tgccgactcg ttcgcccagt
     3541 agttgaagcc cttccacctt ggggctgata cgcgcgctgg cgcgagccgc cagggccgca
     3601 ccgagtacgt gcgaaggggt aaacaccgcg gttgcaccga tcttttccat cggcggacgg
     3661 tgcagcggat cctcggccag cgccaggatg ggcccggtat agccgcgttc cctggccatc
     3721 atgatgaagg tcgcatcggc gtgatcatcg gcgttggtga tcagcgcttg cgcagcctcg
     3781 acgccgctca gcatcccggg ctgctcgtcc agtgcgccga agaccacgtc gtagccgcga
     3841 tcccgcaggc gacgggcctg gcgttcatcc tgttccagaa cgacgaaagc cgtgccgacg
     3901 cggcgcagct cctcgaccag cgaatccacc gccgggccgt agcgatggat gagcacacgt
     3961 ccggccatgt cgggc
//
LOCUS       DAORPM010000229         3971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7141_length_3971_cov_18.607252, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000229 DAORPM010000000
VERSION     DAORPM010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7141_length_3971_cov_18.607252"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLD53_10095"
     CDS             <1..1185
                     /locus_tag="PLD53_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRC61308.1"
                     /translation="FYLIAQMVGAGQLIKLLFGLDYNVAIIGVGILMMVYVTFGGMVA
                     TTWVQIIKACMLLFGGTLAMLLAFSQFGFSFHTLVEKATSTHALGFKLMTPGGLLSNP
                     INAISLGLGLMFGTAGLPHILMRFFTVTDAKEARKSVLYASGFVAYFFNVIFLMGLCA
                     ILIVGQNPEFFEGGTIGGSLIGGGNMVAMHLAKAVGGDMLLGFMAAVAFATILAVVSG
                     LALAGASAISHDLYANVFKKGKATEASEIRVSRIATICLGVVAIILGILFEKQNVAFM
                     VGLAFGVAAAANFPVLILSMFWRGLTTRGALFGGYGGLFSAVTFVVLSKSVWVDVLAN
                     PAPLFPYTQPALFAMPIAFLLAFIVSKSDSSVRAKSEIAAFEDQYIRGQTGFGASGAS
                     KH"
     gene            complement(1273..1644)
                     /locus_tag="PLD53_10100"
     CDS             complement(1273..1644)
                     /locus_tag="PLD53_10100"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRC61309.1"
                     /translation="MLKSKLHRVTTTHAELHYEGSCAIDEDLLDAANIREYEQIDIWN
                     VNNGERFTTYAIRAERRSGIISVNGSAARKAAPGDILIIASFAVYNEVELAKYAPALI
                     YFDAQNRIARQGSSIPAQAAA"
     gene            complement(1736..2560)
                     /gene="panC"
                     /locus_tag="PLD53_10105"
     CDS             complement(1736..2560)
                     /gene="panC"
                     /locus_tag="PLD53_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRC61310.1"
                     /translation="MQIHSAIPDLRHALRNHGRVVFVPTMGNLHDGHLSLMVQAREHG
                     HTVVASIFVNRLQFGPNEDFEKYPRTFEADCEKLREADIDLLFAPTEADLYPERQQYY
                     VDPPGIQNQLEGEVRPGHFRGVATVVLKLFNIVQPQVAVFGKKDYQQLMILRNMTRQL
                     ALPIEIVGGETVRADDGLALSSRNGYLSPSERSEAPRLYRILSGIREAVLAGDKDFER
                     LERAATVELDSHGWKTDYVAIRQQSDLQVPLAGNNPLVVLAASRLGTPRLIDNIDI"
     gene            complement(2595..3404)
                     /gene="panB"
                     /locus_tag="PLD53_10110"
     CDS             complement(2595..3404)
                     /gene="panB"
                     /locus_tag="PLD53_10110"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRC61311.1"
                     /translation="MSTHSATRRLTQLDLAKMRADGEKIAVLTCYDASFAQLCDAAGV
                     EVLLVGDSLGMVLQGHDSTLPVTVDDIAYHTRSVACGAKHALVAADLPWSSYQESPVQ
                     AYRNAARLMAAGAQMVKLEGGIEMAETVAFLTQRGIPVWAHVGLTPQYVHTLGGYRVQ
                     GRGDAAAKVMADAKAHVAAGAAMLLLEAIPSDLAAKVTTENRAPTIGIGAGKDCSGQV
                     LVLHDMLDVTPGKKPRFVKNFMDGQRSIASAIAAYVAAVKDGSFPGPEHCY"
     gene            complement(3408..>3971)
                     /locus_tag="PLD53_10115"
     CDS             complement(3408..>3971)
                     /locus_tag="PLD53_10115"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRC61312.1"
                     /translation="EHLDAELLLEQPETNPFLSRFYQNQQRYALQTQLFFLFHRLDQL
                     HERTQLQLFPRTVVSDYLLEKDLLFAGLTLNEDEYKLYRQIYDRLSPQASVAPDLVIY
                     LQAKPETLIARVRKRGIDMERRISDDYLLLLGESYTRFFHHYDDAPVMVVNSENLNFA
                     DSTDDFLLLVQRIESMRGRREYFNRGD"
BASE COUNT          783 a         1092 c         1173 g          923 t
ORIGIN      
        1 ttctacctga tcgcccagat ggtcggcgcc ggccagttga tcaagctgct ttttgggctc
       61 gattacaatg tcgctatcat tggcgtgggt atcctgatga tggtctatgt gacctttggc
      121 ggtatggttg ccacgacctg ggtgcagatc atcaaggcct gcatgctgct gtttggcggc
      181 acgctggcaa tgctgctggc cttcagccag ttcggattct ctttccatac ccttgtcgag
      241 aaggcgacgt cgactcatgc gctggggttc aagctgatga caccaggcgg tctgttgtcc
      301 aatccgatca atgccatttc cctaggcctt ggtctgatgt tcggtaccgc cggcctgcca
      361 cacatcctga tgcgtttctt caccgtgacc gatgccaagg aagcgcgcaa gtcggtgctc
      421 tatgcatccg gcttcgtagc ctacttcttc aacgtgattt tcctgatggg cctttgtgcc
      481 atcctgatcg tcggccagaa ccctgagttt tttgaaggcg gcacgatcgg cggcagtctg
      541 atcggcggtg gtaacatggt cgccatgcat ttggccaagg cggttggcgg cgacatgctg
      601 ctcggcttca tggcagccgt tgccttcgcg accatccttg ctgtggtttc cggactggcg
      661 ctggcaggcg catcggcgat ttcgcacgat ctctacgcca acgtgttcaa gaaaggcaag
      721 gccacggaag cttcggagat ccgcgtttcg aggatcgcca ccatctgtct cggtgtcgtg
      781 gccatcatcc tcggcatcct gttcgaaaag caaaacgtcg cattcatggt cggcctggcc
      841 ttcggtgtag cggctgcggc caacttcccg gtgctcatcc tttcgatgtt ctggagaggc
      901 ctgaccacac gtggagcact tttcggcggt tatggcggcc tcttttccgc cgtcaccttc
      961 gtcgtgcttt ccaagtcggt atgggttgat gtgctggcca atccagcgcc gctgttcccc
     1021 tatacccagc cggcactttt cgccatgccg atcgctttcc tgctggcctt catcgtctcg
     1081 aagtctgatt cgagcgtaag ggcaaagtcc gaaattgcag cgttcgaaga tcagtacatt
     1141 cgtggtcaga cgggcttcgg agcatcggga gcaagcaagc actaaagacg attcaccaac
     1201 aaaaagccct ggccggtcta cccgccaggg cttttttatt acgcgagaag cggaacgtcg
     1261 gggagcgtcg gttcaggcgg ccgcttgcgc tggtatcgaa ctaccttggc gcgcgatgcg
     1321 gttttgtgcg tcaaagtaga tcagcgccgg tgcgtatttc gcgagttcga cttcgttgta
     1381 gaccgcaaaa ctggcaatga tgagaatgtc gcctggcgca gctttgcgcg ccgccgaacc
     1441 attcaccgag atgatgcccg accgacgttc ggcacgaatg gcgtaggtcg tgaaacgctc
     1501 accattattg acattccaga tatcgatctg ctcatactcg cggatgttgg ccgcgtcgag
     1561 caggtcctcg tcgatagcgc atgatccttc ataatgaagc tccgcgtggg ttgtggtcac
     1621 gcggtgcagc ttcgacttca gcatcgttct ctgcatggtt ttgcccgtgc aagttgtgtt
     1681 gggggatacg cattgtaacg gctaatctca ggctgtcaag gccgtgttgc agagattaaa
     1741 tgtcaatgtt atcaatgagt ctcggtgtgc caagtctgct ggcagcaagc actacgagcg
     1801 gattgtttcc ggccagtgga acctgcaggt cggattgctg gcgtattgca acgtagtcgg
     1861 tcttccagcc gtgactgtcg agttcgacag tggccgcacg ttcgagtctc tcgaagtcct
     1921 tatcgccagc aagtaccgct tcacggatac cgctcaggat gcggtaaaga cgtggagctt
     1981 cgctccgctc ggacggtgac aggtaaccat tgcgcgacga aagcgccagg ccatcatcgg
     2041 cacgcaccgt ttcgccgccg acgatttcaa tgggcagcgc cagttgtcgt gtcatgttgc
     2101 gcagaatcat cagttgctgg tagtccttct tgccgaagac ggcaacctgc ggctgtacga
     2161 tattgaagag tttgagtacg acggtcgcaa cgccccggaa atgaccggga cgcacttcac
     2221 cctcgagctg gttctggata ccgggcggat cgacataata ttgctgccgt tcggggtaga
     2281 ggtcggcctc ggtcggcgcg aacagcagat caatgtccgc ttcccggagt ttttcgcagt
     2341 cggcttcaaa ggtgcgcggg tatttctcga aatcctcatt cggaccgaac tgcagacgat
     2401 tgacaaaaat gctggcgacg accgtatgcc cgtgttcgcg cgcctgcacc atcagcgaga
     2461 ggtggccatc gtgcaggtta cccatggtcg ggacgaagac cacgcggcca tggtttctca
     2521 gggcgtggcg caggtcggga atggcggagt gaatctgcat ggggttttgt gcttcttggt
     2581 tgaaagaggt tgagtcaata acaatgttcc gggccgggga agctgccgtc cttgaccgcc
     2641 gcgacataag cggcgatggc agaggcgata gaccgttgac cgtccatgaa gttcttgaca
     2701 aagcgtggtt tcttgcctgg ggtgacatcc agcatatcgt gcaggacgag gacttgcccc
     2761 gagcaatcct tgcctgctcc gatgccaatg gttggagcgc ggttttcagt ggtgaccttg
     2821 gcggccaggt cagacggaat cgcttccagc agcagcatgg ccgcgcccgc tgcaacgtga
     2881 gctttggcat ccgccatgac tttggcggct gcgtcacccc gaccctgcac acgatagccg
     2941 ccgagagtgt gcacatactg cggcgtcagg cctacatgcg cccagacggg aatgccgcgc
     3001 tgcgtcagaa atgcaacggt ctccgccatt tcgataccgc cttccagctt caccatctga
     3061 gctccggcag ccatcagcct ggcggcgttg cggtaggcct gaacaggtga ctcctgatag
     3121 gacgaccacg gcaagtccgc tgcgaccaaa gcgtgtttgg ctccgcacgc tacgctgcgg
     3181 gtatggtagg caatgtcgtc aacggtcacc ggcagcgttg aatcgtggcc ttgaagcacc
     3241 atgccgagcg aatcgccaac cagcaggact tcgaccccag cggcatcgca caactgcgcg
     3301 aagctcgcat cgtaacaggt gagaacggcg atcttttccc cgtccgcccg catctttgcc
     3361 aggtcaagtt gcgtcaatcg tcgtgttgcg gaatgcgtgg acatggatta gtcccctcta
     3421 ttgaagtatt cgcggcgacc gcgcatggat tcgatacgct ggacgagcag caaaaaatca
     3481 tcggtgctgt cagcaaagtt gagattctcg gagttaacca ccattaccgg tgcatcatcg
     3541 tagtggtgaa agaaccgcgt atagctctcg cccaggagca acaggtagtc gtcgctgatc
     3601 cggcgctcca tgtcgatacc gcgcttgcgg acacgagcaa tcagcgtttc tggtttggcc
     3661 tgcaggtaga ttaccagatc gggtgcaacg gaagcctggg gcgaaagtcg atcatagatt
     3721 tgacggtaaa gtttgtactc gtcttcgtta agggtcaggc cagcgaacaa caggtccttc
     3781 tccagcaggt aatcactcac cacggttcgc gggaagagtt gaagttgagt acgttcatgc
     3841 aattggtcaa ggcgatggaa aaggaagaaa agctgtgtct gcagtgcgta gcgttgctgg
     3901 ttctgataaa aacggctgag aaatggattg gtttccggct gctcaagcaa taattcggcg
     3961 tccagatgtt c
//
LOCUS       DAORPM010000230         3971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7143_length_3971_cov_10.380490, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000230 DAORPM010000000
VERSION     DAORPM010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7143_length_3971_cov_10.380490"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLD53_10120"
     CDS             complement(<1..411)
                     /locus_tag="PLD53_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRC61313.1"
                     /translation="MNQTELEALAAQVGALLVANGQKLVTAESCTGGWVAQCLTAIAG
                     SSTWFDRAFVSYSNEAKREMLGVTGETLSTHGAVSEATAIAMARGALYNSHADWALAI
                     TGVAGPSGGSPGKPVGTVCFAWAGPENGESCETCH"
     gene            complement(408..905)
                     /locus_tag="PLD53_10125"
     CDS             complement(408..905)
                     /locus_tag="PLD53_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRC61314.1"
                     /translation="MIKASFKPPSLSFLLSHPAHLVACGFGSGLSPFAPGTAGTLFAW
                     ALFPILREWTSDVELLGILVACFIGGVLAAHKTGADLGVVDHGSIVWDEIVPFWLVLV
                     FCPASFLWQALAFLLFRFFDIVKPQPARYFDEEVKNGFGVMTDDLIAAGYTVLVLALL
                     KFILQ"
     gene            complement(886..1860)
                     /gene="thiL"
                     /locus_tag="PLD53_10130"
     CDS             complement(886..1860)
                     /gene="thiL"
                     /locus_tag="PLD53_10130"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRC61315.1"
                     /translation="MPSEFDLIARYFVRPTPNAILGPGDDCAVLAPAPGMELAITTDM
                     LVSGTHFLPDADPCQLGWKTLAVNLSDLAAMGAKPRWALLAGSLPSADEPWLAAFADG
                     LFACAERYGVDLVGGDTTRGPLNLCVTALGEVPAGAALRRDGAQVGDDLWVSGQPGLA
                     ALGLAHLQGRARLPEPLASRCLAALHRPLPRIELGLALRRLAHAMIDVSDGLLADLGH
                     IAERSGVVAEVFQPQLPALPTGAEPALVRNCQLAGGDDYELVFSAAPGKRHELAELAA
                     QLDLPLWRFGRIIKAGGGIHGEVRLLDEHGQTLPTTRKGFDHFDQGKL"
     assembly_gap    1946..2045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2046..2519)
                     /gene="nusB"
                     /locus_tag="PLD53_10135"
     CDS             complement(<2046..2519)
                     /gene="nusB"
                     /locus_tag="PLD53_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRC61316.1"
                     /translation="MSNPRDHVPTAANGVKREPQPATRSSAKPLAKSPRRRAREFALQ
                     GLYQWRISGNDEATIKAHMGDVAGFQKADREFFTGLLHGVLAQHAALQEQLQTFLDRP
                     FSELSPVEASVLLAGAYELANFPQTPYRVIINEAIELTKGYGGTDGHKYVNGVLDK"
     gene            complement(2516..2998)
                     /gene="ribH"
                     /locus_tag="PLD53_10140"
     CDS             complement(2516..2998)
                     /gene="ribH"
                     /locus_tag="PLD53_10140"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRC61317.1"
                     /translation="MARYDNIFEYENDLAGDGLAVGIVMSRFNQDICEGLLAACTAEL
                     KRLGVADDDIEIATVAGALEIPLVLQTMAQSDRYDALIALGAVIRGDTYHFEVVANDA
                     CRAVMEVQLDTGIPIANGILTCDDDDQALARMQVKGADCARTAIETANLLRAIGERPA
                     "
     gene            complement(3016..>3971)
                     /locus_tag="PLD53_10145"
     CDS             complement(3016..>3971)
                     /locus_tag="PLD53_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRC61318.1"
                     /translation="ARGLICLTLTEARCKQIGLTQMARDNKSAYSTAFTVSIEAASGV
                     TTGISAQDRARTIQVAVARDARPEDIVQPGHIFPITAKAGGVLVRAGHTEAGCDLAQI
                     AGLDPSSVICEILKDDGSMARLDDLITFAQAHSLKIGSIADLINFRSRNETLVERAFS
                     KPVQSVHGDFTLHAYTDRSTNEVHLALTKGEICADKETLVRVHEPLTVLDFLDPSSGR
                     HSFSLDQARHALSNADCGVIVLLRRPETCHDILAALVEQSPTQRPAAKWDPRMYGIGA
                     QILRDLGVRKMKLLASPRRMPSMTGFDLEVTGYISSPAEFE"
BASE COUNT          789 a         1143 c         1200 g          739 t
ORIGIN      
        1 gtggcaggtt tcgcagcttt caccattctc cggcccggcc caggcgaagc atacagtgcc
       61 gaccggtttg cccggcgaac ctccgctcgg accggcgaca ccggtaatgg cgagcgccca
      121 gtcggcatgg ctgttgtaca gcgctcctct ggccatggcg attgccgtgg cctcgctgac
      181 tgcgccgtgt gtgctgagcg tctctccggt tacgcccagc atctcccgct tggcttcgtt
      241 cgagtacgag acgaaagcgc gatcaaacca cgtcgaactg cccgcaatcg cagtcaggca
      301 ttgagcaacc cagccccccg tacaggactc ggccgtcacc agtttctggc cgttggctac
      361 caacaaagcg ccgacttgtg cggcgagggc ttcgagttct gtctggttca ttgcaggatg
      421 aatttcagga gcgcgagtac gaggacagtg tagccagcgg caatcaggtc gtcggtcatc
      481 acaccaaagc cgtttttcac ttcttcatcg aaatagcgtg ccggttgcgg tttgacgatg
      541 tcaaaaaaac ggaaaagcag aaaggcgagc gcctgccata ggaaactcgc cggacaaaac
      601 accagaacca gccagaaggg aacgatttcg tcccaaacga tgctgccatg atcgacaacg
      661 ccaagatcgg cgcctgtctt gtgcgctgca agcacgccgc caatgaagca cgcgaccagg
      721 ataccgagca gttcgacgtc gctcgtccat tcccggagaa ttggaaacag cgcccacgcg
      781 aaaagcgttc cagcggttcc aggtgcaaaa ggcgacaggc cgctgccgaa tccgcatgcg
      841 acaagatgtg ctggatgcga caagaggaag ctgagcgatg gtggtttaaa gcttgccttg
      901 atcaaaatga tcgaatcctt tgcgagttgt gggcaaggtc tgtccatgtt cgtccagtag
      961 acggacttcg ccgtgaatgc caccccctgc cttaataata cgaccgaagc gccacaacgg
     1021 caggtcgagc tgtgccgcca gctcggccag ttcatggcgt ttgccgggtg cggcagaaaa
     1081 taccagttcg taatcgtcac cgccagccag ttggcaattg cgtaccagcg cgggttccgc
     1141 tcccgtaggc agcgcaggaa gctgtggctg gaatacttcg gcgacaacgc ccgagcgctc
     1201 ggcgatatgg cccagatcgg caagcaggcc gtccgacaca tcgatcatgg catgcgccag
     1261 cctgcgcaaa gccaatccca gctcaatgcg tggaagtggc cggtgcaagg ccgcaaggca
     1321 acggctggcg aggggttccg gcaatcgtgc tcgaccttgc agatgcgcca gtccaagagc
     1381 cgccaatccg ggctgtccgg acacccaaag gtcatcgccc acctgtgcac cgtcccggcg
     1441 tagcgcggcg ccggccggca cctcgccaag cgcggtgacg cacagattga gcggcccgcg
     1501 cgtggtatcc cctcccacca gatcgacgcc gtagcgttcg gcgcatgcaa agagcccgtc
     1561 agcaaaggcg gcaagccaag gctcgtcggc gcttggcaga ctgccggcga gcaatgccca
     1621 acggggcttg gcgcccattg cagccagatc ggagaggttg accgccaggg tcttccagcc
     1681 cagctggcag ggatcggcgt cgggaaggaa atgggtgccg gagaccagca tgtcagtcgt
     1741 gatcgccagt tccataccgg gtgcgggcgc cagcactgcg caatcgtcgc caggaccgag
     1801 gatcgcgttt ggcgttgggc gcacgaagta gcgggcgatc agatcgaatt cggagggcac
     1861 gaaaagaacc gattaaccac agagacacgg agacacagag tattcgcaga gaagagcaag
     1921 cccgaaatgg ggtgctctgt gtctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnntttgt cgagcacgcc gttgacgtat ttgtggccat cggttccgcc gtaacccttg
     2101 gtcagttcga tggcttcgtt gatgatgacg cgataggggg tctgcgggaa attggcgagt
     2161 tcgtaggcgc cagccaacag cacactggcc tcgacaggtg aaagttcact gaacgggcga
     2221 tcaaggaagg tctgaagctg ttcctgtaaa gccgcatgtt gcgcaagaac gccgtgcagc
     2281 aggccggtga aaaactcgcg atcggccttt tgaaacccgg cgacgtcgcc catgtgcgcc
     2341 ttgatcgtcg cctcatcgtt gccactgatc cgccattggt agaggccttg cagcgcaaac
     2401 tcacgagcgc ggcggcgcgg tgacttggcg agcggttttg cagacgatct ggttgcgggt
     2461 tgaggctcgc gcttgacccc attggcggcg gtagggacgt gatcccgtgg gttgctcatg
     2521 ccggcctttc gccaattgcg cgcagcagat tggccgtctc gatggcggtc cgggcacagt
     2581 cagcgccctt cacctgcatg cgcgcgagcg cctgatcgtc gtcgtcgcaa gtcagaatgc
     2641 cgttggcaat cgggatgccg gtatcgagtt gcacctccat gactgcgcgg catgcgtcgt
     2701 tggcaacgac ttcgaagtga taggtgtcgc cgcgaatgac ggccccgagg gcaatcaagg
     2761 catcgtaacg gtcgctctgt gccatcgttt gcagcaccag gggaatttca agcgcaccgg
     2821 ccacggtggc aatttcgata tcgtcatcgg ctacgccaag acgcttgagc tcggcggtgc
     2881 aggcagccag caggccttcg caaatatcct gattgaagcg gctcatgacg ataccaacgg
     2941 ccaggccatc accggcaagg tcgttttcgt attcaaaaat gttgtcgtag cgagccatat
     3001 cgatcccctt tcaacttatt cgaattcggc cggtgagctg atataaccgg tcacttcaag
     3061 gtcaaagccc gtcatgctcg gcatgcggcg cggacttgcc agcagcttca tcttgcgcac
     3121 gccgaggtcg cgcaggatct gcgctccgat gccatacatt cgtggatccc attttgccgc
     3181 cgggcgttgc gtgggtgact gctcgacgag cgcggcaaga atatcgtggc aggtttccgg
     3241 gcggcgcagc agaacgatga cgccgcagtc ggcatttgac agcgcgtgtc gcgcctggtc
     3301 gagcgagaag gagtggcggc cactcgaagg gtcaaggaag tcgagcaccg tcaggggttc
     3361 atgcacccgt accagcgttt ccttatccgc gcagatttcg cctttggtca gcgccagatg
     3421 aacttcattg gtgctgcggt cggtataggc gtgcaaggtg aaatcgccat ggacggactg
     3481 aactggtttg gagaaggcgc gttcgaccag cgtttcattg cggctgcgaa aattgatcaa
     3541 gtcggcaatc gaaccgatct tcagactgtg cgcttgcgca aatgtaatca gatcgtccag
     3601 gcgggccatc gaaccgtcgt ccttgaggat ttcgcaaatc accgatgagg gatccagtcc
     3661 ggcgatctgc gcgagatcgc aacctgcctc ggtgtggccg gcacgcacca gaacgccacc
     3721 cgccttggcg gtgatcggaa aaatgtgccc cggctgcacg atatcttccg gtcgtgcgtc
     3781 gcgtgccacg gcgacctgga tcgtgcgtgc cctgtcctga gccgaaatgc cggtggtgac
     3841 accgcttgcc gcctcaattg agacggtgaa ggctgtgctg tacgcgctct tgttgtcgcg
     3901 tgccatctgt gtgagtccga tctgcttgca gcgcgcctcg gtcagcgtca ggcaaatcaa
     3961 gcctcgcgca t
//
LOCUS       DAORPM010000231         3964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7165_length_3964_cov_13.934254, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000231 DAORPM010000000
VERSION     DAORPM010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7165_length_3964_cov_13.934254"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="PLD53_10150"
     CDS             complement(<1..110)
                     /locus_tag="PLD53_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HRC61319.1"
                     /translation="MAVTIEAVQAVLNSLIDPNTKKDFVSTRSAKNIKVDG"
     gene            247..2370
                     /gene="metG"
                     /locus_tag="PLD53_10155"
     CDS             247..2370
                     /gene="metG"
                     /locus_tag="PLD53_10155"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRC61320.1"
                     /translation="MTRRILVTSALPYANGAIHLGHLVEYIQTDIWVRFQKMQPKDKV
                     KECWYVCADDTHGTPIMLRAEQDGITPEALIERVHGEHSRDFAGFHVAFDNFYTTHSE
                     ETHDCANEIYFRLKTAGLIEKRTIEQYYDPVKQMFLPDRFIKGECPKCGAKDQYGDNC
                     ESCGAAYTPNDLINPYSAISGAKPELRNSDHYFFKLSDPRCQGFLRRWTREAGRLQPE
                     AANKMQEWLGFDEGEGGANKLTDWDISRDAPYFGFEIPDAPGKYFYVWLDAPIGYMGS
                     FKNLCSRLGVSFDDYWKAGSPTELYHFIGKDILYFHALFWPAELEHAGYRTPTGIFAH
                     GFLTVDGAKMSKSRGTFITAESYLQQGLNPEWLRYYYAAKLNGTMEDIDLNLEDFTAR
                     VNSDLIGKYVNIASRCAGFIGKKFDGMLAATDAPWLKPLREVATSIADAYEARDYGRA
                     LREIMALADAANVFVNDRKPWELAKQDGREAELHATCSEAIEAFRLLTLYLKPVLPKV
                     AEAVEIFLGIDPLTWSAAAVPLPAGHAINAYSHLMTRIDPKQIAALVEASKESLPSAT
                     DVTPQKTPAKPAKEVAVSTTAGEPGDACISIDDFMKVDLRIARVVDAGHVEGAEKLIR
                     LSLDIGEDKPRQVFAGIKSAYDPAQLVGRLTVMVANLAPRKMKFGLSEGMVLAASSDA
                     MNANGKSGIFLLSPDDGAQPGMRVK"
     gene            2426..2647
                     /locus_tag="PLD53_10160"
     CDS             2426..2647
                     /locus_tag="PLD53_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61321.1"
                     /translation="MLYAVFDVAGMLMFATGAMWLARRQALFIPDFPVNMTEAIVVSV
                     GGLLMMLWAASRILREMITRPASQSNKGQ"
     gene            2716..>3964
                     /locus_tag="PLD53_10165"
     CDS             2716..>3964
                     /locus_tag="PLD53_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRC61322.1"
                     /translation="MNELPLIDWILFTALGWLLIGVIGVAFPRNFLLNSRVLFPLGAI
                     CGLALALLALTGIYASPQVRILPLGMPGLPFHLRLDALSSFFLLLLGTASAGISIYAA
                     GYMRKGDGTAPGLQCLLYHVFLASMAFVMLADDGYGFMVAWETMALSSFFLVTSEHRH
                     PEIRRAGYLYLLVAHVGAVGILLSFGVMADSAGDYSFDAMRDFSRIGFWPTIAFLLAL
                     FGFGAKAGMLPLHIWLPEAHPAAPSPVSAMMSGVMLKTAIYGMLRVSFDLLHAQLWWW
                     GVLLLVLGLISALFGVVFSAVQTDMKRLLAYSSIENIGLIFLGIGLTLIFKSFLLPHL
                     AALALTATLYHCLNHAFFKSLLFLCTGSVLHATRERNLGKLGGLIHRMPWVAWLALIG
                     VLAASGLPPLNGFVSEWLLLQSFL"
BASE COUNT          806 a         1212 c         1079 g          867 t
ORIGIN      
        1 ccatcgacct tgatgttctt tgccgagcgg gtcgaaacga agtccttctt cgtattggga
       61 tcaatcagac tattcaaaac tgcctgcacg gcttcaatcg ttactgccat tctgcactcc
      121 ttgtcaatat acccgagtaa ttttgtacag tttaaccgaa aacgggaggg actgcacttt
      181 gctagaatgc ccgcttggcc gcaaacggct caaccaatga attgcctgaa cccctgatcg
      241 caacccatga ctcgcagaat actcgtcacc tccgcactgc cctacgccaa tggcgcaatc
      301 caccttggtc acctcgtcga gtacatccag accgatatct gggttcgctt ccagaaaatg
      361 cagcccaagg acaaggtcaa agagtgctgg tacgtctgcg ccgatgatac ccacggcacg
      421 ccgatcatgc tgcgcgccga acaggacggc atcacgccgg aagcgttgat cgaacgcgtc
      481 cacggcgagc attcgcgtga cttcgccggt ttccacgtcg cattcgacaa tttttacacg
      541 acgcattccg aagaaacgca cgattgcgca aacgagatct atttccgcct gaagacggct
      601 ggtctgatcg agaaacgcac aatcgagcag tactatgacc cggtcaagca gatgttcctg
      661 ccggatcgct tcatcaaggg tgaatgcccg aaatgcggcg ccaaggacca gtatggcgac
      721 aactgcgaaa gttgtggcgc ggcctacacg cccaatgacc tgataaatcc ctactcggcg
      781 atttccggcg ccaagcccga actgcgcaat tcggaccatt actttttcaa actctccgac
      841 ccgcgttgcc agggctttct gcgccgctgg acgcgcgaag ccggccgact gcagccggaa
      901 gccgccaaca agatgcagga atggctcgga ttcgacgaag gcgagggcgg cgcaaacaag
      961 ctcaccgact gggatatttc gcgcgacgca ccctactttg gtttcgaaat ccccgatgcg
     1021 cccggcaagt atttctacgt ctggcttgat gcaccgattg gctacatggg ttcgttcaaa
     1081 aacctctgca gtcgcctggg agtcagtttt gacgactact ggaaagcggg ttcgccgacc
     1141 gagctctatc actttatcgg caaggacatc ctgtatttcc atgcgctgtt ctggccagcc
     1201 gaacttgaac acgcgggcta ccgtacaccg acgggaatct tcgcacacgg ctttttgacc
     1261 gtcgatggcg ccaagatgtc caagtcgcgc ggcaccttca tcaccgccga gagttacctg
     1321 cagcagggcc ttaatcccga atggctacgg tattactacg ccgccaagct gaacggcacg
     1381 atggaggaca tcgacctcaa ccttgaggat ttcaccgcac gcgtcaattc ggacctgatc
     1441 ggcaaatacg tcaatatcgc cagccgttgc gccggcttca tcggcaaaaa gttcgatgga
     1501 atgctcgcag cgaccgacgc accttggctg aagccgttgc gagaagtcgc gacgagtatt
     1561 gccgacgcct acgaggcgcg cgactatggc cgcgcgctgc gcgagatcat ggcgctggcg
     1621 gacgcggcca atgttttcgt caatgaccga aaaccctggg aactggcaaa acaggacggc
     1681 agggaagctg aactgcacgc tacctgctcg gaagcaatcg aagccttccg cctgcttacc
     1741 ctttatctca agccggtatt gcccaaagtt gccgaagctg ttgaaatctt cctcggcatt
     1801 gacccgctga cctggagcgc cgccgcggta ccgctgccgg ctggtcacgc gatcaacgcc
     1861 tacagccacc tgatgacacg catcgaccca aaacagattg cagcgctggt cgaagccagc
     1921 aaggaatcgc tgccatcggc tacggacgtc acgccgcaaa agactccagc caaacccgcg
     1981 aaggaagtag ccgtgtcaac aacagccggc gaaccgggcg atgcatgtat cagcatcgac
     2041 gacttcatga aggtcgactt gcgtattgcg cgagtcgtcg atgccggcca tgtcgaggga
     2101 gcggagaaac tgatccggct ttcccttgat atcggggaag acaagcccag acaggttttt
     2161 gccgggatca aatcagccta cgatccggcg caactcgtcg gacgcctgac ggtcatggtc
     2221 gccaatctcg cgccgcgcaa gatgaagttc gggctcagcg aggggatggt gctcgcagct
     2281 tcgagtgatg ccatgaacgc aaacggcaag tccggcatct ttctgctatc accggatgac
     2341 ggtgcacaac cgggaatgcg cgtaaaataa cccggccatg cgaccagcac atcaaactcc
     2401 gcgcccgcga ccgactctga gaataatgct gtacgctgtc ttcgatgtgg ccggcatgct
     2461 gatgtttgct accggagcaa tgtggctcgc tcgcaggcag gcgctgttca tccccgactt
     2521 ccccgtcaat atgacggagg cgattgtcgt aagtgtcggt ggcctgttga tgatgctctg
     2581 ggccgccagc cggattttgc gcgagatgat cacgcgcccc gctagtcaaa gcaacaaagg
     2641 tcaatagtcc gtgcccgcga agccgtcctc agccaccagg tttctcacgg caagcgtttg
     2701 cgtgggtgag aaaaagtgaa cgaactgccg ttaatcgact ggatcctttt cacggcactg
     2761 ggctggttgc tgatcggcgt gatcggcgtt gcgttcccgc gcaatttttt gctgaacagc
     2821 cgcgtcctct ttcccctcgg cgcaatttgc ggtttggcgc ttgcattgct tgcactgacc
     2881 ggcatttacg ccagtccgca agtccgcatc ctgcccctcg gcatgcccgg cctgcccttc
     2941 catttgcgcc ttgacgctct gtcctccttc ttcctgctac tgctgggaac agcctcagcc
     3001 ggcatttcga tctacgccgc cggctatatg cgcaagggcg acggaacggc tccggggctg
     3061 caatgcctgc tctatcacgt ctttctggcc agcatggctt tcgtcatgct ggccgacgat
     3121 ggttatggct tcatggtcgc ctgggaaacg atggcgctca gctccttctt cctggtcacg
     3181 tcggaacacc gccatccgga aatccgacgt gccggttatc tctacctgct ggttgctcac
     3241 gtcggcgcgg taggcatcct tctttccttt ggcgtgatgg cggacagcgc gggcgactat
     3301 tcctttgacg ccatgcgcga tttctcccgc atcggtttct ggccgaccat cgcttttcta
     3361 cttgcgcttt tcggcttcgg cgccaaggcc ggcatgttgc cgctacacat ctggctaccc
     3421 gaagcgcacc cggccgctcc ttctcccgtg tcggcgatga tgagtggcgt catgcttaaa
     3481 acagcaatct acggaatgct gcgggtgagc tttgatctgt tgcatgcgca actatggtgg
     3541 tggggcgttc tgctgctcgt cctcgggctg atttcagcac tgttcggcgt cgtcttctcg
     3601 gcggtacaaa ccgacatgaa acgactgctc gcatattcgt cgatcgagaa catcgggttg
     3661 atatttctcg gaatcggact gacactgatc ttcaagtcct tcctgctacc gcatcttgct
     3721 gcactggcgc taaccgccac gctctatcat tgcctgaacc acgccttctt caagagcctg
     3781 cttttcctgt gtaccggctc ggttctgcac gccacgcgcg agcgcaacct gggcaaactc
     3841 ggtgggctga ttcatcgcat gccatgggtc gcctggttgg cgctgatcgg cgtgctggct
     3901 gcatccggtc tgccgccact taacggtttc gtctcggagt ggctgttgct gcaaagcttc
     3961 ctgt
//
LOCUS       DAORPM010000232         3964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7166_length_3964_cov_6.674853, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000232 DAORPM010000000
VERSION     DAORPM010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7166_length_3964_cov_6.674853"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="PLD53_10170"
     CDS             <1..1433
                     /locus_tag="PLD53_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRC61323.1"
                     /translation="RYAGFMDEVCKLVVDKYDGSLKAEHGTGRNMAPFVEMEWGRQAT
                     DLMRRIKKLFDPENRLNPGVILNDDPAAHLKHLKPMPAAEDIVDRCIECGFCEPLCPS
                     HQLTLSPRQRIVSWRELSRRSTAGEAAGELGADYPYFGLDTCAGCGLCSTACPVGINT
                     GDLTRRLRGRGQSGIAHKFGRWTGEHFGAVASVSRAGLQLGHLASAVVGDKPLSKISR
                     GAWKTAMPRPGRALRARRGEGDPVVYFPACGGRIFGPSTAGEAQLGDVIQLLLTRAGY
                     APRLPEGYDKLCCGQMLASKGLAEEADAGAARLEAALSEASENGRYPIIMDASACSVR
                     MQTHLSGRLPLFDFHEFAHDALLPRLRLVRQAGPIALHVNCSVRKSGSDTKLQNLVRA
                     CVEQVIEPAGVTCCGFAGDRGFVVPELYQHALRKIHDELPKACDCGVSSNRTCEIGLT
                     AETGRTYRSVAYLLEECSRAGAVGAC"
     gene            1636..2223
                     /locus_tag="PLD53_10175"
     CDS             1636..2223
                     /locus_tag="PLD53_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRC61324.1"
                     /translation="MKALLKLSQFIDSITGFIGRSIIWVILVMTLISAGNAMMRYTIN
                     YSSNAYLEIQWYLFSFVFLMGAGYTFMKNEHVRIDVISHRLSKRAQAWVDIFGIIFFM
                     MPMAIAIMWMSWPIFVLAFDDHEVSSNAGGLVVWPVRLMVPVGFFLLIMQGISELIKR
                     IGFLRGLCPDPTEKPHEPTPEEELALAIKAEKGEL"
     gene            2223..3725
                     /locus_tag="PLD53_10180"
     CDS             2223..3725
                     /locus_tag="PLD53_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRC61325.1"
                     /translation="MTQFLIANMAPIIFASMIMFMLVGFPIAFALAANGVVFGLIGME
                     LGLFGPQLFQALPDRIFGIMSNDTLLAIPFFTFMGLILERSGMAEDLLDTIGQLFGPI
                     RGGLAYAVVFVGALLAATTGVVAASVISMGLISLPIMLRYGYDKKLATGIIAASGTLA
                     QIIPPSLVLIIMADQLGKSVGDMYKGAFIPGFVLSLMYVGYIVLVSIFKPAWAPALPL
                     QARTLRGSKLMLRVLTTMVPPLALIFLVLGTIFMGIATPTEGGAMGATGALIMALARR
                     RLTWKLLKQGMESTAKLTTFVIFILIGARVFSLTFYAVDGHRWVEHLLTGLPGGQVGF
                     LIVVNILVFLLAFFLDFFELSFIIVPLLGPVADKLGIDLIWFGILLAVNMQTSFMHPP
                     FGFALFFLRSVAPPSVKTSDIYWGAIPFVCIQVIMVGLLIAFPQLVTFGLDKDAKVDL
                     DAVEIDVPVSGYGSINLPPSLVAPSGEGAPPKEEDPNKAILDAMKQEQAK"
BASE COUNT          752 a         1131 c         1154 g          927 t
ORIGIN      
        1 cacggtacgc gggcttcatg gacgaagtct gcaagctggt cgtcgacaag tacgacggct
       61 cgctcaaggc cgaacacggc accgggcgca acatggcgcc ctttgtcgaa atggagtggg
      121 gcaggcaggc taccgatctg atgcgtcgta tcaagaaact gttcgatccg gaaaaccgat
      181 tgaatccggg agtcatactg aatgacgatc cggcggccca cctgaagcat ctcaagccca
      241 tgccagcggc cgaggatatc gtcgatcgct gcatcgaatg cggcttttgc gaaccgctgt
      301 gtccctcgca tcagctcacc ctttctccac gtcagcggat cgtctcctgg cgcgagttgt
      361 cacggcgcag tactgcaggc gaagccgccg gtgaactggg tgcagactat ccgtatttcg
      421 ggctcgatac ctgcgcggga tgcggattgt gttcgacagc ctgcccggta ggaatcaata
      481 ccggtgatct gacacgccgc ctgcgcggcc gaggtcaaag cggtattgca cacaagtttg
      541 ggcgctggac gggcgagcac ttcggcgccg tcgcttcggt ttcccgtgcg ggattgcaac
      601 tcggtcactt ggccagcgcg gttgttggcg acaagccgct gagcaaaatc tcccgcggcg
      661 cgtggaaaac ggcgatgcct cgacccggca gggcgctcag ggcacgtcgg ggcgagggcg
      721 acccggttgt ctacttccct gcctgcggag gacggatctt tggcccgagc acggctggtg
      781 aggctcaact cggtgacgtc atccagctgt tgctgacccg cgcgggttac gcgccgcgtt
      841 tgcctgaggg ttacgacaaa ctgtgctgtg ggcagatgct ggccagcaaa gggttggcgg
      901 aggaggccga cgccggtgca gcgcgtctcg aagccgcact cagcgaagcc agtgaaaacg
      961 gacgttatcc gatcatcatg gatgcgagcg cgtgcagcgt gcgcatgcag acgcatttgt
     1021 ccggtcggct gccgctcttc gacttccacg aattcgccca cgacgccctg ttgccccggc
     1081 tacggctagt ccgacaagcc ggcccgattg cactgcatgt caattgcagc gtgcgcaaga
     1141 gcggttcgga taccaagctg cagaatcttg tcagggcgtg tgtcgagcag gtcatcgagc
     1201 cggccggggt gacctgttgc ggcttcgccg gtgatcgcgg ttttgtcgtg cctgaactgt
     1261 accagcatgc cctgcgcaag attcacgacg agctgcccaa ggcctgcgat tgcggcgttt
     1321 caagcaaccg cacctgcgag atcgggctga cggccgaaac cgggcgaacc taccggtccg
     1381 tcgcttacct gctggaggag tgcagtcgcg cgggggccgt aggggcttgc tgattccggt
     1441 tggcatccat gactacacgc acacaagagt ttgtgttcaa gaggaaggag actgatttgc
     1501 caggcagata aatcgacaat ccactgtagt tgttatttgc ttggtggccg gatacattac
     1561 ccagccacat ggcaacgaaa aaactagcgg gaccgagaag cccgagattt caagccaatt
     1621 ttggaggaat attttttgaa agcattgctc aaactctcgc agttcattga ctcgattacc
     1681 gggtttatcg gcaggagcat catctgggtc attctggtca tgacgctgat cagcgccggc
     1741 aacgcgatga tgcggtacac gatcaattac agctccaacg cctatctcga aatacagtgg
     1801 tatctattct cgtttgtctt tctgatgggt gctggctaca cattcatgaa aaatgaacat
     1861 gtgcgcatcg acgtcatctc gcatcgcctc tcgaagcggg cgcaagcctg ggtcgacatt
     1921 ttcggcatca tcttcttcat gatgccgatg gccatcgcca tcatgtggat gtcgtggccg
     1981 attttcgttc tcgcttttga tgaccatgag gtttcgagca acgctggcgg ccttgtagtg
     2041 tggcccgtac gcctgatggt tcccgtcggc ttcttcctgc tcatcatgca gggcatttcc
     2101 gaactgatca agcgcatcgg tttcttgcga ggattgtgtc ctgaccccac ggaaaaaccg
     2161 catgaaccca ccccggaaga agaactcgca ctggccatca aggcagaaaa gggagagctg
     2221 tgatgaccca gttcctcatt gccaatatgg caccaatcat tttcgcttcg atgatcatgt
     2281 tcatgctcgt cggttttccc atagcttttg cgctagctgc caatggtgtg gtcttcggtc
     2341 tgatcggtat ggaactcggc ctgttcggcc cgcagctctt ccaggcactg ccggaccgaa
     2401 tctttggcat catgtcaaac gataccctgc tggccattcc attctttacc ttcatgggcc
     2461 tgattctgga acgctccggc atggccgagg atctgctcga tacgatcggg cagcttttcg
     2521 gcccgatccg cggcggcctg gcgtatgccg ttgtttttgt cggcgcactg cttgcggcaa
     2581 caaccggtgt cgtcgccgcc tcggtcatct cgatggggct gatttcgctg ccaatcatgt
     2641 tgcgctatgg ttacgacaag aaactggcga ccggcatcat cgccgcttcc ggtacactgg
     2701 cacagatcat cccgccatcg ctggtactga tcatcatggc cgaccagttg ggtaagtccg
     2761 ttggcgacat gtacaaaggc gcgttcatcc cgggtttcgt actcagcctg atgtatgtcg
     2821 gctacatcgt cctggtatcg attttcaagc ccgcttgggc acccgccttg cctcttcaag
     2881 cgcgtaccct gcgcggaagc aagctgatgc tgcgcgtgct gaccacgatg gtgcctccac
     2941 tggcgctgat ctttcttgtg ctcggcacca tcttcatggg cattgccaca ccgaccgaag
     3001 gtggtgcgat gggtgccacg ggcgccctga tcatggcttt ggcgcgtcgg cggctgacct
     3061 ggaaactgct caaacagggt atggaatcca cggccaagct aaccaccttc gtgattttca
     3121 tactgatcgg cgcccgtgta ttctctctca cgttctatgc tgttgatggt caccgctggg
     3181 tagagcacct gttgaccggt ctgcctggag gtcaggtcgg cttcctgatc gtggtgaaca
     3241 tcctggtctt tttgctggcg ttctttctcg atttttttga gctttcgttc attatcgttc
     3301 cgttgctcgg gccggttgcc gacaagctgg ggatcgacct gatctggttt ggcatactgc
     3361 tcgcggtgaa catgcagacc tcgttcatgc acccgccgtt cggctttgcg ctctttttcc
     3421 tgcgatcggt tgccccgcca tcggtcaaga cttccgatat ttactggggc gcgatacctt
     3481 tcgtgtgcat tcaggtcatc atggtgggtc tgctgattgc gtttccgcaa ctggtgacct
     3541 ttggcctcga caaggatgcc aaggtcgatc tcgacgcggt ggaaattgac gtaccggttt
     3601 cgggctacgg ttcgatcaac ctgccgccgt cgctggtcgc gccatccggg gaaggcgctc
     3661 cgccgaaaga agaggacccg aacaaggcca tcctggacgc gatgaagcag gagcaggcaa
     3721 agtaattcag gatcatttca gcgcacggta atatccgcag tgttgatcca gccccgcttc
     3781 ggcggggctt tctattttgc actggaggcg aaacagtcaa caaaaacggg agccgaagcc
     3841 cccgtttcca gtccaggaaa aacgattccc ttgccgtaga ttttacttcg cgccgtacat
     3901 gtaattcgcg taggagcctt cggcgatacg gaaccacagg ttttgatccg ccatgaattg
     3961 tttg
//
LOCUS       DAORPM010000233         3950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7209_length_3950_cov_13.439538, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000233 DAORPM010000000
VERSION     DAORPM010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_3950_cov_13.439538"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLD53_10185"
     CDS             complement(<1..407)
                     /locus_tag="PLD53_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRC61326.1"
                     /translation="MMFHTDDPLAIPLWINGHAYLTMAPAFQDVRNPVSREVLRRTPL
                     CGSAEALKAVDAAQAALSAWAAQDAAARAALLASVGEALSGYAAHFARLIMEESGKEA
                     ASADGEVVRAVALLQGAEAGDAADQIDRPGVIG"
     gene            complement(645..1655)
                     /gene="pbpG"
                     /locus_tag="PLD53_10190"
     CDS             complement(645..1655)
                     /gene="pbpG"
                     /locus_tag="PLD53_10190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HRC61327.1"
                     /translation="MKTAVLFVTLLGAGFTVTDNAQAVEKNKSKSVSHKNKAAPVAKK
                     SKPSQVVRKKKASKVSVSGEKQWQETRELAVQSSAALVIEQGGGDELFLKNASEVVPI
                     ASITKLMTAMVVLDSQASLRGQIAITDEDVDTLRGSRSRLRVGSGLTRENALLLALMS
                     SENRAANALARHYPGGMSAFLTEMNIKAKVLGMKDTHFDDPTGLNSSNVSTARDLAKM
                     VVAAYRYPLIREFTTTAEATVDVSGRAMAYRNTNPLVKSAAWDVGLSKTGYIHEAGKC
                     LVMQARVADKPVVIVLLDSAGKQTRVGDANRIRRWMETTQASHRAATHAALSRSSSGP
                     PG"
     gene            complement(1790..2947)
                     /locus_tag="PLD53_10195"
     CDS             complement(1790..2947)
                     /locus_tag="PLD53_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRC61328.1"
                     /translation="MSDSGSSSTSNGRTALVLAGGGARAAYQVGVLLAIRDLMPEPER
                     NPFTILCGTSAGAINAVTLGCYADNFGAAVDALADVWRNFHAAQVYRADPLGIAASGA
                     SWMGTMVFGWLLKRSPRSLLDNTPLRELLEQKLNFRNIGRSIDRGSLYAVSLTASGYT
                     SGQSVSFFQAGADVESWERTHRFSCRAQLNIDHLLASSAIPFIFPAVRLNREYFGDGS
                     MRQLAPISPAIHLGAEKVLIIGAGRMFEPVERQHSTAYPTMAQIAGHALSSIFLDSLA
                     VDIERMQRINLTLAAMPDEVKKRNRIPLRPIQSLVFSPSEELDVLAADHVTALPWPVR
                     FLLRGIGAMSRRGGALASYLLFEGSYTRVLIDLGYKDAMARREEVKAFLEV"
     gene            3320..>3950
                     /locus_tag="PLD53_10200"
     CDS             3320..>3950
                     /locus_tag="PLD53_10200"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235256.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRC61329.1"
                     /translation="MTQTISINAPDYVKHEGLKKWVAEVAALVTPERVVWADGSQEEY
                     DRLCNELVEAGTFTKLKKRKNSFLAFSDPSDVARVEDRTYICSEKKEDAGPTNNWEDP
                     AKMRETLKGVFKGCMKGRTMYVIPFSMGPVGSPIAQIGVEISDSAYVVVNMKIMTRMG
                     KAVFPVLGTDGQYVPCVHSIGAPKEPGQKDPKWPCNPTVKYIVHYPETRE"
BASE COUNT          917 a         1153 c         1051 g          829 t
ORIGIN      
        1 atgccgatta cacccggcct gtcaatctga tccgcggcat cgccggcctc cgcaccctgc
       61 aacaaggcca ctgccctcac cacttcaccg tcagccgaag ccgcctcctt gccgctttct
      121 tccataatca atctggcgaa atgcgcggcg tagccggata gcgcttcacc cacacttgcg
      181 agcagggctg cgcgagctgc agcgtcctgc gctgcccacg ctgaaagcgc ggcctgtgcg
      241 gcgtcgactg ccttgagcgc ctccgctgaa ccgcacagcg gcgtacgccg caatacttcg
      301 cggctgaccg ggttgcggac atcctgaaag gccggggcca tcgtcagata agcgtgacca
      361 ttgatccaca gagggatcgc gagcgggtcg tcggtatgaa acatcatcgt ccttggtaat
      421 cggttctctg catggtacac actttgccgc ggagccgggc ttcaacgcaa aaccgcttat
      481 tgctgacctg tatggatgcg ttgtcaagga gttggatgag ttgggtcgaa ttgatctgga
      541 caaatggggg agactaccga cgatccatat cttactttgc gctatcacct gcggattgca
      601 gcgaagcggg cgaatcagta acggccagtg cgcgacggct aagattagcc tgggggaccg
      661 ctgcttgaac gtgaaagcgc agcatgcgtc gccgctctgt gcgaagcctg agtagtttcc
      721 atccagcgcc tgatcctgtt ggcatcacct acccgggtct gcttgccagc cgaatcaagc
      781 agcacgatga ccaccggctt atcagccaca cgtgcctgca tgaccaggca cttcccggct
      841 tcgtgaatgt atccggtctt ggatagtccg acatcccagg ccgcgctctt caccaatgga
      901 ttcgtgttgc gataggccat tgcgcgcccg ctaacgtcca ccgtcgcctc tgcagtggtc
      961 gtgaattccc tgattagcgg gtagcggtat gccgcgacaa ccattttcgc caaatctcgc
     1021 gcggtggata cgttactact gttcaacccg gtcggatcat caaagtgcgt atccttcatc
     1081 ccaagcactt ttgctttgat gttcatctcg gtgagaaatg cggacattcc accgggataa
     1141 tgtcgagcca gcgcattggc cgccctgttc tctgaagaca tcaatgcgag aagcagagcg
     1201 ttctcgcgtg tcaaacccga acccacacgc aaacgtgaac ggctgccacg cagggtgtcg
     1261 acatcttcat ccgtaatggc gatttgcccc cgcaggcttg cctggctgtc cagcacaacc
     1321 atggccgtca ttagcttggt gattgaggca attggcacga cttcgctggc gttcttcaga
     1381 aagagctcat cgccgccgcc ctgctcaatg accaacgcag cactggattg cacagccaat
     1441 tcgcgcgtct cctgccactg cttttcgcca ctcactgaaa ccttgctggc cttctttttc
     1501 ctgaccactt gcgaaggctt actcttcttg gcgacgggag cggccttgtt cttgtgtgaa
     1561 acagatttgc tcttgttttt ttcgacagcc tgtgcgttat cggtgactgt aaaaccagcg
     1621 cccagcaacg ttacgaacag cacagccgtt ttcatccata accacatcga taatgccccc
     1681 agttgaatct attgagatga tcattccggt tcagtctatc accgggcaaa tcaaaatcaa
     1741 gaaaaataat gagatagggg aaatacttga gagacaacat caaaaatatt catacctcaa
     1801 gaaaggcctt gacctcctcg cgacgagcca tcgcgtcctt atagccgagg tcgatcaaga
     1861 cacgggtata ggaaccctcg aacaacaaat aacttgccaa agcgccaccg cgacgactca
     1921 tcgcaccgat gccccgtaga agaaaacgta ccggccaggg tagcgcggtg acgtggtctg
     1981 ctgccaggac atcaagctct tcggacggag agaagaccag cgattgaatc ggtctaagcg
     2041 ggatccggtt tcgtttcttt acttcatcag gcattgccgc caaggtcaaa ttgatgcgct
     2101 gcattctttc gatatctacg gcaaggctat cgagaaagat cgaagaaagc gcgtggcccg
     2161 cgatctgcgc catagtcggg taagcagttg aatgctggcg ctctactggt tcaaacatcc
     2221 ggcccgcacc gatgatcagc actttttcag cgccgagatg aattgccggg gaaatcggcg
     2281 ccagttggcg cattgagccg tcaccgaaat actcgcggtt gagtcggacc gctggaaaga
     2341 taaagggaat cgcactggac gccaacagat gatcaatatt gagctgcgcg cgacagctga
     2401 agcgatgcgt tcgctcccac gattcaacat ctgcgcccgc ctggaaaaag ctgacgcttt
     2461 gaccgctggt gtatccagag gctgtcagac tgacagcata gagcgaaccg cggtcaattg
     2521 accggccaat gttacggaaa ttcagcttct gttcgagcag ttctcgcaac ggagtattgt
     2581 cgagcagcga acggggcgat cgctttaaca gccagccaaa caccattgta cccatccagc
     2641 ttgcacctga agcagcgatg cccaaagggt cagcgcgata aacctgtgcg gcatgaaaat
     2701 tccgccagac atcggccaga gcatcgactg ccgcgccaaa attgtccgca taacagccca
     2761 gcgtcaccgc attgatggct ccggcggaag tgccgcagag aatcgtaaaa ggattgcgtt
     2821 cgggttcggg cataagatcg cggatggcga gcagaacgcc gacctgatag gctgcccgcg
     2881 cgccgccccc cgcaagaacc agtgctgtcc tgccgttgct cgtcgaagaa gaacccgaat
     2941 cgctcatggt gcatcgaatg atagctggat tggaggttgg cgtagcaaca acgatcatgg
     3001 cgaagccgaa aaggcgccgg cagcgctatg cgccggctcc tatgtgagcg catgttgcac
     3061 tatggaaacg ctcccgaaat tcgacgcgac agcttgaccg gctaaagcta tggtactatt
     3121 tcctaggtca attatcgaag cttccgagtc gaccatgatt gcactgcctc atcaacaatc
     3181 agtcaagtat ttccggcttg gaccgacgtg tcgtctcgca ctggatctgc cagcccatac
     3241 aggatttcac gaagcgattt cctgacgagc aacagcatgg atcccctttt ttcaactgtc
     3301 ctttagagag tgtattgcca tgacccaaac catatccatc aatgcccctg attacgtcaa
     3361 acacgaaggc ctgaagaagt gggttgccga agtcgccgca ctggttacac cggaacgcgt
     3421 cgtgtgggcc gacggctcgc aggaagaata tgaccgcctc tgtaacgagc tggtcgaagc
     3481 cggcactttc accaagctca agaaacgcaa gaactccttc ctggcttttt ccgacccatc
     3541 cgacgttgct cgtgtcgagg accgtaccta catctgttcc gagaagaaag aagacgctgg
     3601 tcccaccaac aactgggagg acccggccaa gatgcgcgag acgctgaagg gcgtgttcaa
     3661 gggctgcatg aagggtcgca caatgtacgt catccccttc tcgatgggtc ccgttggctc
     3721 accgattgcc cagatcggcg tcgaaatcag cgacagcgcc tatgtcgtcg tcaacatgaa
     3781 gatcatgacc cgtatgggta aggcagtgtt cccggtactg ggtaccgatg gccagtacgt
     3841 gccgtgtgtg cattcaatcg gcgcaccgaa ggaacccggc cagaaggatc caaagtggcc
     3901 gtgcaacccg accgtcaagt acatcgtgca ctatcccgaa acccgcgaaa
//
LOCUS       DAORPM010000234         3950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7210_length_3950_cov_9.789730, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000234 DAORPM010000000
VERSION     DAORPM010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7210_length_3950_cov_9.789730"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="fghA"
                     /locus_tag="PLD53_10205"
     CDS             <1..693
                     /gene="fghA"
                     /locus_tag="PLD53_10205"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HRC61330.1"
                     /translation="GLTCTEETFMIKAGAQRIAAELGLALIAPDTSPRGAGVTGETES
                     WDFGVGAGFYLDATESPWSTHYRMESYLVAELIPLLAQALPIKARRLGVFGHSMGGHG
                     ALTLSLRHPGLFKSVSAFAPVCAPKQCPWGQKAFAGYLGPDTALWSAHDASALMGACT
                     KAPYPEGILIDQGLADKFLPDQLHPHLFEQACARAGQPLTLRRHEAYDHGYYFISTFM
                     ADHLAHHARILL"
     gene            718..2295
                     /locus_tag="PLD53_10210"
     CDS             718..2295
                     /locus_tag="PLD53_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61331.1"
                     /translation="MPTSAQHPYHPIIYVRGFAGTQGEIEETVADPYMGFNIGSTKAR
                     MAWTGDVKRYFFESPLVRLMSDHEYGDVFVDGEDLVTADRPDCVIPYRSIIIYRYYDE
                     ASKDFGTGETPPIEHFAKGLGTLILRLRDKVCANPKNAMTPEDFRVHLVAHSMGGLIC
                     RAFLQNTALRSEEARHAVDKLFTYATPHNGIDMRIVRNVPGWLSFGDANNFNRERMAA
                     YLAVPPVDDVSVVFNFPPERIFNLVGTNPRDYTVAGGLSAWGAGDASDGLVRIENATT
                     HGPDAKGNDVSSPRAFVNRSHSGHYGIVNSEEGFQNLTRFLFGALRVDGILDIDDITL
                     PIEVQKELQAGKAVHSSYQFEVAASIRGCQWQMTRREVREHSAIFRTYEELFPGAAGT
                     TRPPDRTRSPRLFSVFLDPNKSVKASGSVSFAFDLKVLVPDYEVDGLLFMKRHYEGGY
                     IMRELILVEAFPDKSALGGWRIKYGFQDDNPGKPGISAETRELDGEEGIAFDIPIAQR
                     TRPGVKGTLRLEARTWS"
     gene            complement(2480..2662)
                     /locus_tag="PLD53_10215"
     CDS             complement(2480..2662)
                     /locus_tag="PLD53_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61332.1"
                     /translation="MITPETDACYLIALCSGEERRWRYLGQDARGATWWRDLETELEF
                     SESSLMYAWTVVERLG"
     gene            complement(2679..>3950)
                     /locus_tag="PLD53_10220"
     CDS             complement(2679..>3950)
                     /locus_tag="PLD53_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRC61333.1"
                     /translation="VMVIDAAKGVEAQTIKLLNVCRMRNTPIITFVNKFDREVREPFE
                     LLEEIESVLGIECAPITWPIGMGKTFRGVYHLLEDSLLRFAPGEERRSEAEKLDGIGN
                     PVLDAQFPQEVGKLREDVDLIQSASGPFNLVDFLAGLQSPVFFGSGINNFGVQEILQA
                     LLDWAPPPQERDAGIRTVQPAEPPFTGFVFKIQANMDPKHRDRIAFFRVCSGRYSSGM
                     KVRHVRMNREMKLANALTFMANERVLMEDAVAGDIIGIHNHGQLHIGDTLTEGETLAF
                     KGIPYFSPELFRAARLRDPLKSKQLQKGLQELGEEGAIQVFENLATGALLLGAVGTLQ
                     FEVVAQRLQTEYKVDAIFEAADIHTARWLTFPDELTRRNFEREQQHRMAKDVDGNLVY
                     LASTRYNLDVTSEKWSKVGFHDSREHGQVLA"
BASE COUNT          786 a         1233 c         1162 g          769 t
ORIGIN      
        1 ggtctgacat gcaccgagga aaccttcatg atcaaggccg gtgcgcaacg cattgccgcc
       61 gagctgggtc tggcgttgat tgcgccggat accagcccgc gcggggccgg cgtcacgggc
      121 gaaaccgaga gctgggactt cggcgtcggc gcaggattct atctggatgc caccgagtcg
      181 ccgtggtcaa cgcactaccg catggaatcc tacctcgtcg ccgaattgat tccgctgctt
      241 gcgcaagcgc taccgatcaa agcccggcgg ctgggagtgt tcggccactc gatgggcggc
      301 catggcgcac tgaccctgtc gctcagacat cccggactgt tcaagtcagt ctcggcgttt
      361 gcacccgtct gcgctccaaa gcaatgcccc tggggtcaaa aagcgtttgc cggctacctt
      421 ggccccgaca ccgcgctctg gtcggcgcat gatgccagcg ctctcatggg cgcatgcacc
      481 aaagcgccct accccgaagg catcctgatc gaccaggggc tggccgacaa gtttctaccc
      541 gatcaactgc atccgcatct gttcgagcag gcttgcgcca gggccgggca accgcttacc
      601 ctgcgtcgcc acgaggcgta tgaccacggg tattatttca tctcgacctt catggcagac
      661 catctggcac accacgcccg gatactgctc tgagcaccgc accaaaagga gaatgtcatg
      721 ccgacttcag cgcagcatcc ctaccatccg atcatctacg tacgcggttt cgcaggcacg
      781 cagggcgaga tcgaggagac cgttgccgat ccgtacatgg gcttcaacat tggctcgacc
      841 aaggcgcgca tggcctggac cggcgatgtg aagcgctact tcttcgaatc gccgctggtt
      901 cgcctgatga gcgatcacga atacggcgac gtcttcgtgg acggtgagga tctggttacc
      961 gccgaccgcc ccgactgcgt gatcccgtat cgcagcatca tcatctaccg ctattacgat
     1021 gaagccagca aggacttcgg caccggcgag acgccaccca tcgagcactt cgccaagggt
     1081 ctgggcacgc tgatcctgcg cctgcgcgac aaggtctgcg ccaacccgaa gaacgcgatg
     1141 acgcccgagg attttcgcgt gcacctggtg gcgcactcga tgggcggact gatctgccgc
     1201 gccttcctgc aaaacacggc gttgcgcagc gaagaagcgc gccatgcggt tgacaaatta
     1261 ttcacctacg cgacgccgca caacggcatc gacatgcgca tcgtgcgcaa tgtgccgggc
     1321 tggctttcct tcggcgatgc caacaacttc aaccgcgagc ggatggccgc ttatctcgct
     1381 gtgcccccgg tcgacgacgt ttcggtcgta ttcaatttcc cgcctgaacg catcttcaat
     1441 ctggtcggca ccaacccgcg cgattacacg gtggccggcg ggctctcggc ctggggagcg
     1501 ggagacgcta gcgacggact ggtgcgcatc gagaatgcca ccacgcacgg gccggatgcc
     1561 aaaggcaacg atgtgagctc gccgcgcgcc ttcgtcaacc gcagccactc ggggcactac
     1621 gggatcgtga attccgagga aggttttcag aacctcaccc gcttcctgtt tggtgcgcta
     1681 cgcgtggacg gcattctcga catcgacgac attaccctgc cgattgaagt acaaaaggag
     1741 ttgcaggccg gcaaggccgt gcattcctct taccagttcg aggtagcggc cagcatacgc
     1801 ggctgccaat ggcaaatgac gcgccgcgag gtacgcgagc attccgcgat tttccgcacg
     1861 tatgaagagc tgttccccgg cgcggccgga accactcgcc cgcctgatcg taccaggagc
     1921 ccgcgactgt tttccgtttt cctcgacccg aacaagagcg tcaaggcatc gggctcggtg
     1981 agctttgcct tcgacctcaa ggtgctcgta cccgactacg aggtggacgg cctcctgttc
     2041 atgaaacggc actacgaggg cggctacatc atgcgcgagc tgatcctggt cgaagccttc
     2101 cccgacaagt cggcactcgg gggctggcga atcaagtacg gtttccagga cgacaatccg
     2161 ggcaagccgg gcatttccgc cgaaacgcgc gaactcgacg gtgaagaggg tatcgccttc
     2221 gatatcccga ttgcgcaacg aacccgacca ggcgtgaaag ggacgctacg ccttgaggcg
     2281 cggacatgga gttagtggtc agtcatgcca cgaaacaagg acaaaaccca ccaccaagac
     2341 acaaagtaaa cagagtttca ccaatagaat cccagtgtta acttggtgct tcttggtgaa
     2401 cttggcgact tggtggttcg cctttctcgt tgactgacta ccagtccggc cagccactac
     2461 gaagccatgc cgagcgatgt cacccaaggc gttcgacaac ggtccacgcg tacatcaaac
     2521 tgctttcact gaattccagc tcggtttcaa ggtcacgcca ccacgtcgcg ccgcgagcat
     2581 cctgaccgag atagcgccag cggcgttctt cgccgctgca tagcgcaatg aggtagcagg
     2641 cgtcggtttc cggcgtgatc acagaagcat cgctcggctc aggcaagcac ctgcccgtgt
     2701 tcgcgggaat cgtggaagcc gaccttgctc cacttctcgc tcgtgacgtc gaggttgtag
     2761 cgcgtgctgg ccagatacac caggttgccg tcgacgtcct tcgccatgcg gtgctgctgc
     2821 tcgcgttcga aattgcggcg cgtgagttca tccggaaatg tcagccagcg ggcggtatga
     2881 atgtctgctg cctcgaagat ggcgtcaacc ttgtattcgg tttgcaggcg ctgggcaaca
     2941 acctcgaact gcaaggtgcc gacggcgccc aggaggagtg cgccggtggc gagattctca
     3001 aaaacctgga ttgcaccttc ttcacctagt tcctgaagac ctttctgtaa ctgtttgctt
     3061 tttaatgggt cccgaagacg cgcagcgcgg aaaagctccg gcgagaaata ggggatgccc
     3121 ttgaacgcca gcgtttcacc ctcggtgagc gtatcgccga tatgcaactg gccgtggttg
     3181 tgaatgccga tgatgtcgcc ggccacagcg tcctccatca gcacgcgctc gttggccatg
     3241 aaggtcagcg cattggcgag cttcatctcg cggttcatgc gcacgtgccg caccttcatg
     3301 cccgaactgt agcgacccga acagacgcgg aaaaaggcga tgcggtcgcg gtgcttcggg
     3361 tccatgttgg cctggatctt gaacacaaaa ccggtaaatg gcggttcagc cggctgcacg
     3421 gtgcgaatgc cggcatcgcg ctcctgtggc ggcggcgccc agtcgagcag tgcctgcagg
     3481 atttcctgca cgccgaagtt gttgataccg gagccgaaga agaccggtga ctgcagcccg
     3541 gcgagaaaat cgaccagatt gaacgggccg ctcgcactct ggatcaggtc gacgtcttca
     3601 cgcagcttgc cgacctcttg cgggaattga gcgtcgagca ccggattgcc gatgccgtcg
     3661 agtttttccg cttcgctacg ccgttcttcg cccggcgcga aacgcagtaa actgtcttca
     3721 agcaggtgat acacgccgcg gaaggtcttt cccatgccga tcggccaggt aatcggcgcg
     3781 cactcgatgc caagcaccga ttcgatttct tcgagcagct cgaaaggctc gcgcacctcg
     3841 cggtcgaact tgttgacgaa ggtgatgatc ggcgtgttgc gcatgcggca aacgttgagc
     3901 agcttgatcg tctgcgcctc gacgcctttc gcggcgtcga ttaccatcac
//
LOCUS       DAORPM010000235         3906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7348_length_3906_cov_6.977928, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000235 DAORPM010000000
VERSION     DAORPM010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7348_length_3906_cov_6.977928"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..949)
                     /gene="nhaR"
                     /locus_tag="PLD53_10225"
     CDS             complement(41..949)
                     /gene="nhaR"
                     /locus_tag="PLD53_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HRC61334.1"
                     /translation="MINYKHLHYFVSVARAGGVIRAAERLHLTPQTLSGQISQFEERL
                     GVALFRRVGRRLELTETGKLALSYAEEIFQTGAELEDLLKNGAEERFITFRVGIADVV
                     PKFTAHRLLAPVLGLPTAIRLICQEDRLDRLLADLAIHRLDMVLADRPMPPGMEIKGY
                     SHPLGESGVAFMAAPALAARLTGDFPDCLDGAPLLLPGRDSALHGALPRWLERKGKRV
                     RIVGEFDDSALMKAFGEAGAGIFPTPAASVPEVTAHYQVVKLGETEEIRERFFLISAE
                     RRLTHPAARAVSENARSGMIAPPGAV"
     gene            1067..1423
                     /locus_tag="PLD53_10230"
     CDS             1067..1423
                     /locus_tag="PLD53_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRC61335.1"
                     /translation="MNLKIQAQDLPLTDVLRQHVATRLAYALNHGRDVVTRIVVRLSD
                     VNGPRGGKDKCCGIEVRVKGAPVLIVEDTQADLHVAIDRAAERIGRTLDRQLARRRTF
                     VAVSATRHQAPSMEKE"
     gene            1548..2228
                     /locus_tag="PLD53_10235"
     CDS             1548..2228
                     /locus_tag="PLD53_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRC61336.1"
                     /translation="MNHLGTQAVAQPRRGAETVLAANKVIRNTYMLLSMTLLFAAVTA
                     AASVAMKLPHPGMIITLVGYFGLLFATHKLKNSGWGLVSVFALTGFMGYTLGPIVGHY
                     LGLPSGGQTVMMAMAGTAAIFLGLSGYALTTRKDFSFMGGFLMVGILLAFLAGLTAVF
                     FEIPALSLTVSAAFVLLMSGLILFETSNIIHGGETNYVMATVTLFVSIFNLFTSLLHL
                     LGFMNSND"
     gene            2221..2415
                     /locus_tag="PLD53_10240"
     CDS             2221..2415
                     /locus_tag="PLD53_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61337.1"
                     /translation="MTDEALLATLSPSHGLVPASARAWVPGRHEFRRILVLPGAAGCN
                     AGADVLGTAAAGVALQRWAR"
     gene            2573..3655
                     /locus_tag="PLD53_10245"
     CDS             2573..3655
                     /locus_tag="PLD53_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRC61338.1"
                     /translation="MVVVYLVLGLVLLVAGADLLVRGASRLALRFGISPLLIGLTVVA
                     FGTSAPELAVSVQAGLAGQSGIAVGNIVGSNIFNVLMVLGVSALITPLVVSQQLIRVD
                     VPLVIGASVLFLIMAFDGRIGLFDGLLLTGGIVAYIVFSIRQGRKESAAVQAEYAQDF
                     GGEASLLLGQLPVQIGLIVGGLALLVLGATWLVDSAVSIARILGVSEVIIGLTIVAAG
                     TSLPELATSVVAAMRGERDIAVGNVIGSGLFNIFAIGGIAAMVTPGGLEVAPALIRFD
                     IPVMVAVALACLPIFATGHCIARWEGALFVGYYLAYVAYLVLAATQHAALPAYSDAML
                     GFVLPLTAVTLVVLLARQRAAMRVAP"
     gene            3652..>3906
                     /locus_tag="PLD53_10250"
     CDS             3652..>3906
                     /locus_tag="PLD53_10250"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HRC61339.1"
                     /translation="MTRAKSSPLLDLLVTIIVPSVILMKLSDPESLGAINALLVALAF
                     PLSWGARSLLRERRVNPFAALGLVSVLLTGGIGLLQLDTQW"
BASE COUNT          639 a         1227 c         1232 g          808 t
ORIGIN      
        1 gcaagactgg cgcagttcaa caaactgcga cacgggcttt tcaaacggcg ccaggcggcg
       61 cgatcattcc gctgcgcgca ttctcgctga ccgcccgcgc cgcgggatgg gtcagccgcc
      121 gctcggctga aatgaggaaa aaccgctcgc gaatctcctc cgtttcgccc agctttacga
      181 cttggtagtg cgccgtcacc tccggcacac tggccgccgg cgtcgggaat atcccggcgc
      241 cggcttcgcc aaaagccttc atcagcgcgc tgtcgtcgaa ttcgccgacg atgcgcacgc
      301 gcttcccctt tcgttccagc cagcgcggca gggcgccgtg aagtgcgctg tcacgcccgg
      361 gcagcagcag gggtgcgcca tccaggcaat ccgggaagtc gcccgtcagc cgcgcagcca
      421 gcgcgggcgc agccatgaaa gccacgcctg attcgccgag cgggtggctg tagcccttga
      481 tctccatgcc cggtggcatg gggcggtcgg ccagcaccat gtcgagacgg tggatcgcca
      541 gatccgccag cagccggtcc agccggtcct cctggcagat caggcgaatt gccgttggaa
      601 gtcccagcac cggcgccagc aggcgatggg cggtgaattt cggaacgacg tcggcgatgc
      661 cgacgcggaa ggtgatgaag cgctcctcgg cgccgttctt cagcaagtct tccagctcgg
      721 ccccggtctg gaagatttcc tcggcgtagg agagcgccag ttttcccgtc tcggtcagtt
      781 ccagccggcg cccgacgcgc cggaacagcg ccacgcccaa ccgttcctcg aactgggaaa
      841 tctgccctga gagcgtctgg ggcgtcagat gcaggcgctc cgcggcgcgg atgacaccgc
      901 ccgcacgggc gacgctgacg aagtaatgca aatgcttgta gttgatcatg ccgctctctc
      961 actgttcgta tttttcgatc ataacgcata gttatatctg tttgtatcga atcgtgacct
     1021 tgcccataat ccaggcgtct tcttccaatc cagcagagga acgccaatga acctgaagat
     1081 ccaagctcaa gacttaccct tgaccgacgt attgcgccag catgtggcca cgcgcctcgc
     1141 ctacgccctg aatcacggcc gggatgttgt cacgcgcatc gtcgtgcgcc tttccgatgt
     1201 caatggcccg cgtggcggca aggacaagtg ttgcggcata gaggtgcgcg tcaagggcgc
     1261 tcctgtcctg atcgtcgagg atacgcaggc cgatctgcac gttgccatcg atcgcgccgc
     1321 cgagcgtatt ggccgtacgc tcgaccggca gttggcgcga cgacgcacat tcgtcgcggt
     1381 ctcagcgacc cggcaccagg cgccttcaat ggaaaaggag tgaagagatg agaagctatc
     1441 ccacaggccg tgaggaatcg atcgcgcgcg caatggtagt gcgctgactg tgccgccccg
     1501 ccagcgccga cccttgaaac cgtttctttc aaacacagga gactgaaatg aaccatctag
     1561 gaacccaagc cgttgcccag ccgcggcgcg gcgcggaaac cgtccttgcc gccaacaaag
     1621 tgatccgcaa cacctacatg ctgctgtcga tgacgctgct gtttgccgcg gtcaccgcag
     1681 ccgcctcggt ggcgatgaag ctgccgcatc ccggaatgat cattaccctg gtcggctatt
     1741 tcggcttgct gttcgccacc cacaagctga agaacagcgg ctggggcctg gtctcggtgt
     1801 tcgcgctgac cgggttcatg ggctacaccc tcggcccgat cgtcggccac tacctcggcc
     1861 tgcccagcgg cggccagacg gtgatgatgg ccatggccgg tacggcggcg atcttcctcg
     1921 gcttgtccgg ctacgcgctg actaccagga aggatttcag cttcatgggt ggttttctca
     1981 tggtcggcat cctgctcgcc ttcctcgcgg gactgacggc ggtcttcttc gagattccgg
     2041 cgctgtcgtt gacggtatcc gccgccttcg tgctgctgat gtcgggcctc atcctcttcg
     2101 aaaccagcaa catcatccat ggcggcgaaa ccaactatgt gatggcaact gtgaccctgt
     2161 tcgtatcgat cttcaacctg ttcaccagct tgctgcactt gctgggtttc atgaacagca
     2221 atgactgatg aagctcttct cgccactcta tcgccgagcc atggtctggt cccggcatcc
     2281 gcacgcgcct gggtacctgg gcggcatgag tttcgccgaa tcctcgttct tcccggtgcc
     2341 gccggatgca atgctggcgc cgatgtgctt ggcacagccg cagcgggcgt ggcgcttcaa
     2401 cgctgggctc ggtgatcggt gtgctgaggt gtatcgctga catgaccgcg accttgaata
     2461 accgagtcgc tatcgatggc cgccaacggg cacgcttctg ggttgagtac ccgcgcgtgc
     2521 gtagcgaagc ggatttgcag gattgctttt tcaagagaac gcaatgagtg tgatggttgt
     2581 tgtctatttg gtacttggtc tggtgctgct ggtcgccggt gcggatctgc tggtgcgcgg
     2641 cgcctcgcgc ctggccttgc ggtttggcat ctcaccgctg ctgatcgggc tcaccgtggt
     2701 cgccttcggc accagcgcgc cggagctggc tgtgagtgtc caggccggcc tggcgggcca
     2761 gtcgggaatc gctgtcggca acatcgtggg cagcaatatt tttaatgtgt tgatggtgct
     2821 tggcgtttcg gcactcatca caccgctggt ggtgtcgcag caactgatac gggttgacgt
     2881 gccgctggtg atcggggcgt cggtgctgtt cttgatcatg gcgttcgacg gccgcatcgg
     2941 tcttttcgac ggcctgctgt tgactggcgg catcgtggct tacatcgtgt tcagcatccg
     3001 gcaaggccgc aaggaaagcg ccgcagtgca ggccgagtat gcgcaagact tcggtggcga
     3061 agccagcctg ttgctggggc aactgccggt tcagatcggg ctcatcgtcg gcggcctggc
     3121 gctgctggtg cttggcgcca cctggttggt cgacagcgcg gtttcgatcg ctcgcatact
     3181 gggtgtgagc gaagtgatta tcggcttgac catcgtcgcc gccggcacgt cattgccgga
     3241 gctcgcaacc tcggtcgtgg cggcgatgcg cggcgaacgc gacattgcgg tgggcaacgt
     3301 aatcggcagt ggcctgttca acattttcgc gatcggcggc attgccgcca tggtcacacc
     3361 gggcggactc gaggttgccc cggcgctgat ccgcttcgat atcccggtaa tggtcgcggt
     3421 ggcgctcgcc tgcctgccga tattcgccac cggccattgc attgcacgct gggaaggggc
     3481 gctgttcgtc ggctactacc tggcctacgt agcgtatctg gtcctcgcgg cgacgcagca
     3541 tgcagcactg cctgcataca gcgatgccat gctcgggttt gtgctgccgc tcacggcggt
     3601 gacgctggtt gtattgctgg cgcgccaacg ggcggcgatg agggtagcac catgacccgg
     3661 gcgaaaagca gcccgctgct cgaccttctg gtcaccatca tcgtcccctc cgtgatcctg
     3721 atgaagctca gcgatccgga aagcctgggc gccatcaacg cgctgctggt ggcactggct
     3781 ttccctctgt cctggggcgc gcggtcgctg ctgcgcgagc gccgcgtcaa cccgtttgcc
     3841 gcgctgggcc tggtcagtgt cctgctcacc ggcggcatcg gcctgctgca actggatacg
     3901 cagtgg
//
LOCUS       DAORPM010000236         3868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7474_length_3868_cov_6.717808, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000236 DAORPM010000000
VERSION     DAORPM010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7474_length_3868_cov_6.717808"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLD53_10255"
     CDS             complement(<1..495)
                     /locus_tag="PLD53_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61340.1"
                     /translation="MPGQPSRIRRVLDSGLVLFALLSSPLVWAETPAPPEIPIPQTLE
                     QADAQRDRAKQLRDEADRNYESEQKACYKKFLVSGCLDEAKQRHTQSMIHARELDIPA
                     RDFQREAKRAEVEAKEAERAADIPSREAEQKEQAEKYRAEEAAKAAKRAQKKADKELK
                     AAENR"
     gene            complement(605..1402)
                     /gene="cobA"
                     /locus_tag="PLD53_10260"
     CDS             complement(605..1402)
                     /gene="cobA"
                     /locus_tag="PLD53_10260"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRC61341.1"
                     /translation="MTPIQRYDKAGTVYLVGSGPGALDLMTVRATRLIGEADVIVHDH
                     LIADGVLDLVRSDAQKICVGKRTSNHTVPQDELNRLLVRLAREGKMVVRLKGGDPFIF
                     GRGGEEIETLFECGIPFEVVPGVTAAAGCAAYAGIPLTHRDHAQTVSFATGHLKDGSV
                     DLDWQALARPRQTSVFYMGIGGIKEICSQMMAHGLHGTHPAAVVQNGSTARQRVVTAD
                     LATLPARVAEAGITSPALIIVGTVVSLHKKLAWYDPVPEPEARNMLK"
     gene            1698..1997
                     /locus_tag="PLD53_10265"
     CDS             1698..1997
                     /locus_tag="PLD53_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRC61342.1"
                     /translation="MLQSGFAHAESPTDSPSPERQRALVHLVRQDCGSCHGMTLQGGL
                     GPALTPEALRDKPVDSLVAVVYSGRPGTPMPPWKRFMTEPEAAWIVEQLMEGFPN"
     gene            2037..2471
                     /locus_tag="PLD53_10270"
     CDS             2037..2471
                     /locus_tag="PLD53_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61343.1"
                     /translation="MEIVTHIVAADDDEYQAVGDSINPLDEWSGIEAPRLDTIKIATL
                     HSLLTGDPLQVALDLYEPVYVAENETVVLRIADELMEKLATLDEDELENVAIEVAATE
                     EFETEQWDTEDIQEQLILLGDLAQLAESQGQALFVWMCLLQD"
     gene            2582..3748
                     /gene="dinB"
                     /locus_tag="PLD53_10275"
     CDS             2582..3748
                     /gene="dinB"
                     /locus_tag="PLD53_10275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRC61344.1"
                     /translation="MKYTAHMYSNRRIAHLDMDAFFASVELLHYPVLRGRAVVVGGRG
                     VMQPQCQPDGSRRFARLRDYVGRGVVTTSTYEARAMGVFSGMGLMKSARLAPDAILLP
                     ANFDAYRDFSRRFKVAVASIASKIEDRGIDEVYVDLSDTTEETNVLATRIKKAVFDAT
                     GLTCSIGISPNKLLSKICSDLEKPDGITILAMADIPTRIWPLPVKKVNGIGPKSTGKL
                     ARLGINTIGELAATPVEVLVKHFGRTAGVWLNAAAHGLDDRPLVIDSEPKSISRESTF
                     ERDLDARKDKAELADIFTSLCMRVADDLKCKGYASKTIGIKLRYADFRAVTRDLTLPV
                     RVSDGAAIRKAAGACLKRVSLAQRIRLLGVRASGLGPVEEEQLITDSVQSELPF"
BASE COUNT          751 a         1036 c         1148 g          933 t
ORIGIN      
        1 gcggttctcg gcggccttca gttccttgtc cgccttcttt tgtgcgcgtt tcgcggcctt
       61 ggccgcttcc tctgcacgat atttctcggc ctgctccttc tgctcggcct cgcggcttgg
      121 aatatctgcg gcacgttctg cctccttggc ttcaacctcg gcgcgctttg cttcacgctg
      181 aaaatcgcgc gccgggatgt cgagttcgcg tgcatggatc attgattgcg tgtggcgctg
      241 ttttgcttcg tccaggcagc cgctgaccag aaacttcttg tagcaggctt tctgttcgga
      301 ttcgtaattt ctgtctgctt cgtcgcgcaa ctgtttggcg cggtcgcgct gcgcatcggc
      361 ctgttcaagc gtctgcggga tcggtatctc gggcggtgcg ggcgtctctg cccagactag
      421 cgggctggat agaagcgcaa aaagcactag accgctgtcg agtactctgc gaatgcgcga
      481 tggctgacca ggcatctggt gtttcctccg agtgtgaata ctgggcgatt ggagtcgggg
      541 tgatcgccta ttctaaagcg tcgcgcttgc tgggaaggcg atggtggcgc gtatgctcgc
      601 ctgactattt gagcatgttc ctggcttcgg gttcgggcac cgggtcgtac cacgccagct
      661 ttttgtgcag actgacgaca gttccgacaa tgatcagcgc cggcgaagtg atgccggctt
      721 ctgcaaccct agccggcagc gtcgcgaggt ctgccgtgac gacccgttgt cttgcggtgc
      781 tgccgttctg gaccacggca gccggatgcg tgccatgcaa gccgtgcgcc atcatctggc
      841 tgcatatttc cttgatgccg ccgattccca tatagaagac cgaagtctga cgggggcgcg
      901 ccaaggcttg ccaatccagg tcgacgctac catccttgag atggccggtg gcgaagctta
      961 cggtttgcgc gtgatcgcgg tgggtgagcg gtattcccgc gtaggcggca caccccgcgg
     1021 cggcggttac tccgggaaca acctcgaagg gtatgccgca ttcgaacagc gtttcgattt
     1081 cctcgccgcc acgcccgaaa atgaaagggt cgccgccttt gaggcggaca accattttcc
     1141 cctcgcgagc cagtcgtacc agtaatctgt tgagttcgtc ctggggtacg gtgtggttcg
     1201 acgtgcgttt gccgacacag atcttctgcg catcgctacg cacaaggtcg agaacgccat
     1261 cggcgatcag atgatcatgg acaatgacat cggcttcccc aatcagacga gtggcgcgga
     1321 cggtcatgag atcaagtgct ccaggcccgc taccgacgag atagacggtt cccgctttgt
     1381 cgtaacgttg aatcggggtc attgaatttt ggctttttga tcgaggtgta agcataagtg
     1441 tcaggcttgg tcagttcaac agttctttct cgaatagccc gcaaatacgc tcgagaaacg
     1501 caattgtttg atatgaatca aaaaagatcg agcatctctc ggcttttcca tggcatgcgt
     1561 tatcatcgcc aaatgatcca gatccagagc aatcagaggt gcttgtgttt caatccccga
     1621 tccatcgtat cgttgtttct tccctcggct tgaaccggtt tcgagggttt ttttctgcgt
     1681 tgttcctcct gtgcctcgtg ctgcaatccg gtttcgccca tgccgaatca ccgactgatt
     1741 cgccgtcacc cgagcgccag cgtgcgctgg tgcatctggt ccgccaggat tgcggctcgt
     1801 gtcatggcat gacgctgcag ggtgggcttg ggccggcact cacgccggaa gcgctgcgtg
     1861 acaaacccgt cgattctctg gtcgctgtcg tctacagcgg tcgcccaggg acgcccatgc
     1921 caccctggaa gcgtttcatg accgagccag aagccgcatg gattgtcgag cagttgatgg
     1981 aagggtttcc caattagtgt ttccgacctg tattttcttg atcaggaatg agtgccatgg
     2041 agatagtgac gcacattgtt gccgccgatg acgatgagta tcaggctgtt ggcgattcga
     2101 tcaatccttt ggacgaatgg agcggcatcg aggcaccccg cctggatacg atcaagatcg
     2161 ccacgctcca ttcactgctg accggtgatc cgctccaggt tgcgcttgac ctttacgaac
     2221 cggtctatgt cgctgaaaac gagaccgtcg tgttgcgcat agcggatgaa ttgatggaaa
     2281 aactggcaac tctcgatgaa gatgagcttg agaacgttgc catcgaggtt gctgcaaccg
     2341 aagagttcga aaccgagcag tgggataccg aagacattca ggaacaactg attctgcttg
     2401 gcgatctggc acaactcgcg gagtcccagg gacaggcact attcgtctgg atgtgtcttc
     2461 tgcaagactg aagttgccac ttcagggtga agtagcgctc ccaattcctc gtactccttc
     2521 gagccctcca tcgaccattt cgtcgggtga ttgccggttt cgaaaggccg caatcggaaa
     2581 tatgaaatat actgcacata tgtacagtaa tcgacgaatc gctcatctcg atatggatgc
     2641 cttctttgcg tctgtcgaat tgctccacta tccggttttg cgggggcggg cagtggtggt
     2701 tggcgggcgc ggcgtgatgc agccacagtg tcagccagac ggcagccggc gttttgcccg
     2761 gttgcgcgac tatgtggggc gaggcgtggt cacaacctca acctatgaag cacgggcgat
     2821 gggggtgttt tccggaatgg gcctgatgaa atcagcccgg ttggcaccgg atgcgatact
     2881 tcttccggcc aatttcgacg cttaccggga tttttcgcga aggttcaaag tggccgtggc
     2941 aagcatcgcg tcaaagattg aggaccgtgg aatcgatgaa gtctacgtcg atttgagcga
     3001 caccacggaa gagacgaatg tcctggccac gcgtatcaaa aaggcggtgt tcgatgcaac
     3061 cggtcttacg tgttccatcg gcatctcgcc aaataaattg ctttcgaaaa tctgctcgga
     3121 tctggagaaa ccggatggca ttacgatcct ggcgatggcc gatatcccta cgcgaatctg
     3181 gccattgccg gtaaagaagg tcaatggcat cggcccgaag tcgactggga aacttgccag
     3241 gcttgggatc aataccatcg gcgaattggc ggcaacaccc gtcgaggttc tggtcaagca
     3301 ctttggtcgt accgccggtg tctggctgaa tgctgcggca catggccttg atgaccggcc
     3361 actggtcatc gactcggaac ccaagtccat cagccgcgag agcactttcg agcgcgatct
     3421 ggacgctagg aaggacaagg cagaactcgc cgatattttc acttcgctgt gtatgcgcgt
     3481 tgccgatgat ctcaaatgca agggctatgc cagcaagacc atcggcatca agttgcgcta
     3541 tgcggatttt cgtgcggtga cccgggattt gaccctgccc gttcgtgtga gtgacggagc
     3601 ggcaattcga aaggctgccg gagcgtgcct gaaacgcgta tctctggcgc agagaatccg
     3661 gctgcttggt gttcgcgcaa gcggtttggg gccggtggag gaagagcagc tcatcaccga
     3721 cagcgttcaa tccgagttgc cgttttaggc ggacatccgg aatcgaccgg gccggaggtt
     3781 tgctgtcccg ggaggtttgg aattttgctg atcaggacat ggcagagttt cttaagttgt
     3841 ttttaagttt gtgggtctta ggatgaac
//
LOCUS       DAORPM010000237         3866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7483_length_3866_cov_9.187352, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000237 DAORPM010000000
VERSION     DAORPM010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7483_length_3866_cov_9.187352"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLD53_10280"
     CDS             complement(<1..251)
                     /locus_tag="PLD53_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HRC61345.1"
                     /translation="MTIKVGINGFGRIGRMVFRAAVQNFSDIEVVGINDLLEPDYLAY
                     MLKYDSVHNRFKGTVSVEGNTLIVNGKKIRLTAVKDPAEL"
     gene            1041..2588
                     /locus_tag="PLD53_10285"
     CDS             1041..2588
                     /locus_tag="PLD53_10285"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRC61346.1"
                     /translation="MAIRKELLAELTARREEAMAGGGLDKMEKRRKKGLLTARERLDD
                     LYDKGSLQELGMHVDHDTAGIDHKRLPGDGVVTGIGNVGGRPVAAFSQDFTVSGGSLG
                     RMHSEKICHMMETAASLGLPVVAINDSGGARIQEGVLSLSGYGQVFFRNVELSGLVPQ
                     IAIIAGPCAGGAAYSPALMDFLIMTRKGSQMFICGPDVIKASTGQEAPIEQFGTADAH
                     ASVSGNIHFVADDDKHALEITRKLLSFLPLNNMQDPPHRIPDTIKVEVDTAMNDLVPE
                     ENRTPFDVSKVIARLVDGGDFLEVQREFAKNIVVGFARIDGMVVGIIANQAMVKAGTL
                     DCDSSDKGARFIRFCNAFNIPIVTLVDIPGFMPGLAQERGGIIRHGAKMLFSYASATV
                     PKITVILRKAYGGAYLAMCSKDLGADFVYAWPSAEIAVMGAEGAVKVIYKKEIDGAED
                     PKAKEMELRARYVKEFASPYQAAEHGLITDVIAPSETRSKVAMALRIALTKRVTRPPK
                     KHGLIPL"
     assembly_gap    2690..2789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2977..3381
                     /locus_tag="PLD53_10290"
     CDS             2977..3381
                     /locus_tag="PLD53_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRC61347.1"
                     /translation="MKMLKITLQGKTYEVGVEFVDGAAPATAAPAPVQAAPAVAVAAP
                     VAAPALAPAPAAAAPAAVAGGQSVTSPMPGLVMKVRVTVGQQVTADQEVLVLEAMKME
                     SPVYAPGAGTVASILVKEGDAVSEGQVLIQLS"
     gene            3389..>3866
                     /locus_tag="PLD53_10295"
     CDS             3389..>3866
                     /locus_tag="PLD53_10295"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRC61348.1"
                     /translation="MSGIIEQFMLMMTQSTGFSHITGGMVIMWAVCVVLFYLAIVKEF
                     EPLLLLPITFGALLANIPTEGVLNAPHVEGLITVPGGLYYYISQGIHLELFPPIIFLG
                     VGALTDFGPLIANPRTFLLGAAAQLGIFMTMFVAVMSGVFTPGEGASIGIIGGADGP"
BASE COUNT          805 a         1018 c         1066 g          877 t
ORIGIN      
        1 agttcggctg gatctttcac tgcggtcagg cgaatcttct tgccatttac gatcagcgtg
       61 ttgccctcga ctgagacagt gcctttaaaa cggttgtgta ccgagtcgta cttcagcata
      121 taggccaagt aatcgggctc gagcagatcg ttgatgccga cgacttcgat gtcgctgaaa
      181 ttctgaactg ccgcacggaa aaccatacgt ccgatacgtc caaaaccgtt gatacctact
      241 ttgatagtca tgattctctt ccctcataaa aaaatcgaac gttgatgata aatgcatttg
      301 caactgttga atattgaact ctgatcttgt caccaagtaa ccggacaaac cagctgcgga
      361 attgaatccc ggcatgggac gccatccgtt ggtagaagtt ccgcgagcga actgaccttg
      421 tgtcgccgac tttgttggga accaccagcc aaggcgaacg attatccgct atctcggagg
      481 atatttccag tgtgcacagg tgaatccatt ccataaagcg aaactggctg atttaactgc
      541 acatggatgc cggagatatt ccggcaaaat ctcgcgggcc gctgagtgtt ggttcgcctc
      601 gtcgcggaaa aatgacattt ctcggttctg tcaggttcga tcagaaacac gtcataatcc
      661 ggtcaggatg agcaaggctc ctttgaccaa tgtctcttcc tttgacaggg gttttctaaa
      721 aaaacaatct tgattgaaaa cgaaaatcaa tatataaatc attgcgccga ttgaccgata
      781 tcggctcggc aacatataat tttttgattt aggttggatt tcctgccttt cctgtgggtt
      841 gcactcatca tcgaatctta catttcggcg aagcagtagt cggacggttc gttgttgcca
      901 cggaaggccg cgcaagacct gcagacaacg gtttttgaca cggtttcaag tgaaggcgtt
      961 ggaatttgga tttcggttaa cctgctccgg cggtgttgta ccgagttttg cacctctcag
     1021 aaataagcca aggagcatgt atggcaatac gcaaggaact attagcagag ttgaccgctc
     1081 gccgcgaaga ggcgatggcc ggaggtggtc tcgacaaaat ggagaagcgt cgcaagaagg
     1141 ggttgctcac tgcacgtgag cgccttgacg atctttacga caagggatcg cttcaggaac
     1201 tcggaatgca cgttgaccat gacacggcag gcatcgacca caaacggttg ccgggcgatg
     1261 gcgtggtgac cggcattgga aacgtgggcg gacgtccggt tgcagccttc tcccaggact
     1321 tcacggtgtc cgggggctcg ctgggccgta tgcactcgga gaagatctgc cacatgatgg
     1381 aaactgcggc cagtctcggg cttcccgtcg ttgccatcaa tgactcgggc ggtgcgcgca
     1441 tccaggaagg tgttctctca ctttcgggct acggtcaggt gttcttccgc aacgtcgagc
     1501 tgtccgggct ggttccccag attgccatca ttgccggccc ctgtgccggt ggcgcggcgt
     1561 acagtccggc gttgatggat tttctgatca tgacccgcaa aggctcacag atgttcatct
     1621 gtggcccgga tgtgatcaag gcctcgaccg gccaggaagc accgatcgaa cagtttggaa
     1681 ccgcggatgc ccatgcctcg gtgagtggca atatccattt cgtggccgat gacgacaagc
     1741 atgcgctgga gatcacacgc aagctgttgt ccttcctgcc gctgaacaac atgcaggatc
     1801 caccgcatcg cattcctgac accatcaagg tcgaagtcga taccgccatg aatgacctcg
     1861 tccctgagga aaacaggacg ccgtttgacg taagcaaagt gattgcccgg ctcgtcgatg
     1921 gcggcgattt cctggaagtt cagcgcgagt ttgcgaaaaa catcgtcgtc ggatttgccc
     1981 gtattgacgg tatggttgtc ggtatcatcg ccaatcaggc gatggtcaag gcgggcacac
     2041 tcgattgtga ttcctccgac aagggtgccc gtttcatccg cttctgcaac gccttcaaca
     2101 tcccgatcgt caccctcgtc gatattccgg gctttatgcc cgggctcgcc caggaacgcg
     2161 gcggcatcat ccgccacggc gccaaaatgc tgttctccta cgcatcggca acggttccga
     2221 agatcacggt catcctgcgc aaggcctatg gcggtgctta tctcgcgatg tgctccaaag
     2281 acctgggtgc cgacttcgta tacgcttggc cgtctgcgga aattgccgtg atgggtgcag
     2341 agggggctgt caaggtgatc tacaagaagg aaatcgacgg cgccgaagat ccgaaagcca
     2401 aggagatgga actcagagct cgctacgtca aggagtttgc ttcgccgtac caggccgccg
     2461 agcacggttt gatcacggat gtgatcgcac cctccgagac gcgttctaaa gtggcgatgg
     2521 cgttgcgcat cgcgctgacc aaacgagtta cccgtccacc caaaaaacat ggactaatcc
     2581 cgttatgagc ctacatgatt cccttgcggt cgttctggcc gttgccaagg acacggcatt
     2641 tgtcgtggca gactcttccg cggtgcagga ggcagcgaaa gcggtctccn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acgaagagct ggttgccatt ctggccgtcg
     2821 ccgcttccga aggcatcggg cagtcggtaa ccgtcgtcaa gtttcggccc atggacagca
     2881 tggactggac atggtcggta cagggccggg taggccttca ctcttcacgc aagctctgag
     2941 cgcagtctca gagtcgaatc ataaaggacg cttaacatga aaatgctcaa aattaccctg
     3001 caaggcaaga cgtacgaagt gggtgtcgaa tttgtcgatg gcgccgcccc cgcgacagcg
     3061 gctcctgctc cagtacaagc ggcgcccgct gttgccgttg ctgcgccggt tgccgcccca
     3121 gctcttgcac cagcgcctgc ggccgccgca cctgcagcgg tagccggtgg tcaatcggtg
     3181 accagcccga tgcccggtct ggtcatgaag gttcgcgtga ccgttggtca acaggtcacc
     3241 gccgaccagg aagttctcgt tctggaagcc atgaaaatgg aatctccggt ttatgcgccc
     3301 ggcgcgggta ctgttgcgtc gatcctcgtc aaggaaggcg atgccgtgtc tgaaggtcag
     3361 gttctgatcc aactaagctg aggttcatat gtccggaatc attgagcaat tcatgttgat
     3421 gatgacccag tcgaccgggt tttcgcatat cacgggcggc atggttatca tgtgggcggt
     3481 ctgcgtcgtt cttttctacc tggccattgt caaagagttc gagcccttgt tgctgttgcc
     3541 gatcaccttt ggcgcgttat tggccaacat tccgaccgag ggcgtgctga atgcgccgca
     3601 tgtcgaagga ctgatcacgg tgccgggggg cttgtactac tacatctcgc aaggcatcca
     3661 tctggagctg tttccgccta tcatattcct cggggtcgga gcactgactg attttggtcc
     3721 gctgatcgcc aaccctcgca ccttcttgct tggtgcggcg gcgcaactgg gtatcttcat
     3781 gaccatgttc gttgctgtga tgagcggggt atttacgccg ggtgaaggcg catcgatcgg
     3841 catcatcggc ggtgccgacg gcccaa
//
LOCUS       DAORPM010000238         3815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7655_length_3815_cov_11.067819, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000238 DAORPM010000000
VERSION     DAORPM010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7655_length_3815_cov_11.067819"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLD53_10300"
     CDS             <1..566
                     /locus_tag="PLD53_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRC61349.1"
                     /translation="VDVAADTPHSPTVYRLIAWAREQSVMRRAADIVAPADLADAARI
                     RRGAGNYDAMCVDCHLSPGVKDSEIRNGLYPTPPNLAGATESTHSSQSDARRFWIIKH
                     GIKASGMPAWSKGGMNDEAIWDLTAFLKALPSLTPETYRRQVEASEGHSHEGLVTRGD
                     KEERTKPVTEQQRDTTPHTHEKGAHKH"
     gene            623..1906
                     /locus_tag="PLD53_10305"
     CDS             623..1906
                     /locus_tag="PLD53_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRC61350.1"
                     /translation="MKIPQRSRSTLFITFSVFASVWCSAAQAQERPPGSDVDSLLIVA
                     KDNNPEYAAIRFEADAASERVAPAGNLPDPKFLTELRDITRTGEQNATLSPSRVSSTK
                     YLLTQALPWYGKRDLKREIAEFEAQGTQGRARSTWAEIGARIKSVFAQLYYVHRNERL
                     TQEILDLIVRLEKIAQVRYAGGLSAQQDVIRAQVEQTSLRNELIALKSERRQLQARMN
                     ALLSRPAEAVLAEPEHLRPVPVPAKLNYTVLEERVRNNNPQLFTEEALIKSAEKNRDL
                     TFKNRYPDFTVGVSPIQHQSSFDQWELMVEVNIPLQQGSRRAQERESEAMLSAAKARR
                     DVTANQVLAELSEYLAGIDAARHTEDLVTNSLLPQADLTFRAALAGYETGKVDFATLL
                     DAQRQIRQARKDQIKARSEMQIRLAQIEKLLGEDL"
     gene            1903..3555
                     /locus_tag="PLD53_10310"
     CDS             1903..3555
                     /locus_tag="PLD53_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC61351.1"
                     /translation="MTQDTGRAMGIIGIVAVALLAGGSGYWFGVRGAAVPDDSEKAIA
                     TTQGESGKKAPKLLYYRNPMGLPDTSPTPKKDPMGMDYIAVYEDEGSTESSEPVSGNQ
                     LRISTDKVQKMGVRTEAAQLRDLGRIVRAAGRIEPDERRVFTISPKFEGYVERLYVNI
                     TGQPVGKGQAMFEVYSPELVSAQREYAIAAQGVEALKDGDNYAQSGMKELAESSLTRL
                     KNWDISEDQVRTLVNSGKSKHTLVFRSPVNGIVTEKKAMQGMRFMPGEALYQVADLSS
                     VWVIADVFEQDIGLVKTGAKAKVTISAYPEMMFEGTITYVYPTLNAQTRTVPIRVELA
                     NPGLLLKPSMFAQVELPVSAKGTVVTVPVSAVIDSGTRQIVLIRQGEGRFEPREVKLG
                     ARSDNHAEVLEGVKDGEQVVVAANFLIDAESNLKAAVGGFGQATHDTAPVEGPATAPA
                     HPTTRGASHHAEGTVGSINAQAGTINLSHGPVASLKWPAMTMEFKAANESLLQGLKPG
                     SKVDVEFVERQPGEWVITAIKPLPASKAAGMPTAGANPHAGH"
     gene            3580..>3815
                     /locus_tag="PLD53_10315"
     CDS             3580..>3815
                     /locus_tag="PLD53_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRC61352.1"
                     /translation="MLANIIEWSGKNRFLVLLATLFITIGGIFAVLRTPIDALPDLSD
                     VQVIVYTEYPGQAPQVVEDQVTYPLTTAMLSVPKS"
BASE COUNT          876 a         1171 c         1129 g          639 t
ORIGIN      
        1 tggtagacgt ggcggccgat acaccccaca gcccaaccgt ttaccgcctc atcgcgtggg
       61 ctcgtgaaca atcggtcatg cgcagggccg ccgacatcgt cgcaccggcc gatctggcag
      121 atgccgcccg gatccgtcga ggcgcgggca attacgacgc catgtgcgta gactgccatc
      181 tctcgcccgg cgtcaaagac agtgagattc gcaacggcct gtacccgaca ccgccgaacc
      241 tcgccggagc gacggaaagc acccattcca gccagtcgga tgcccgccgg ttctggatca
      301 tcaagcacgg aatcaaggcg tccggcatgc cggcctggtc gaagggaggc atgaatgacg
      361 aagccatctg ggatctgact gctttcctca aagccttacc cagcctcacc ccggagacct
      421 atcgcaggca ggtcgaagcc agcgaggggc attcccatga aggtttggtg acgcgcggcg
      481 acaaagaaga aaggacgaaa cctgtcactg aacaacaacg cgacacgaca ccgcatactc
      541 atgaaaaggg tgctcacaag cactgactgc ccaccccgaa ctcttcagac aacacgtttt
      601 gcgaccagga atagacacca gcgtgaaaat accccagcga tccagaagca ccctgtttat
      661 tacgttctca gtgtttgcca gtgtttggtg ctcagcggca caagcacagg agcgccctcc
      721 cggcagcgat gttgacagcc tgctcattgt cgcgaaagac aataatccgg aatatgcggc
      781 aatccgcttt gaagcagatg ccgcaagcga gcgggtagcc ccggcaggca acctcccgga
      841 tccaaaattc ctcaccgaat tgcgcgatat cacgcggacc ggcgagcaaa acgccacgct
      901 ctcgccgagt cgggtcagca gcaccaaata cctgctgacg caggccttgc cctggtacgg
      961 gaaacgtgat ctgaagcggg aaattgccga attcgaagcg cagggtacac aggggcgtgc
     1021 ccgcagcacc tgggcggaga ttggggcacg catcaaatcc gtgttcgcac agctctacta
     1081 tgtgcatcgc aacgaacggc tgacgcagga aatcctcgat ctgatcgtcc gtctggagaa
     1141 gattgcccag gtacgctatg ccggcggcct ttcagcccag caggacgtca tccgggcgca
     1201 ggtcgagcag accagtctgc gaaatgagct gattgccctg aagagcgagc gccggcaact
     1261 tcaggcacgc atgaatgcgc tgctttcgcg ccccgcagag gcagtgctcg ccgagcccga
     1321 acacctgcgt ccggtgcctg tgccggcaaa actcaactac acggtgctgg aagaacgcgt
     1381 gcgcaacaac aacccgcagt tgttcacgga agaagcactc atcaagtcag ccgagaagaa
     1441 tcgtgacctg acgttcaaga accgctaccc cgatttcacg gtcggcgtct cccccatcca
     1501 gcaccagagt tcgttcgacc aatgggagct gatggtcgaa gtcaatattc ccctgcaaca
     1561 gggttcgcgg cgcgcgcagg agcgcgagtc ggaagcgatg ctctctgccg ccaaggcgcg
     1621 acgcgacgtg acggccaacc aggtccttgc cgagctgtct gaatatcttg ccggcatcga
     1681 tgcggcaagg cacacagaag acttggtgac caacagcctg ctgccgcagg ctgatcttac
     1741 cttccgcgcc gcactggctg gctacgaaac gggcaaggtc gatttcgcca ccctgctcga
     1801 cgcacagcgt caaatccgtc aagcccggaa ggaccagatc aaggctcggt ccgagatgca
     1861 gatacgtctg gcgcaaatcg aaaaactttt gggagaagac ttatgacgca ggatacgggc
     1921 cgcgccatgg gcatcatcgg tatcgttgcc gtcgccctgc tggccggcgg gagcggctat
     1981 tggttcggtg tgcgtggcgc ggcggtaccc gacgatagcg aaaaagcgat cgccactacg
     2041 caaggcgaga gcggcaagaa ggcaccaaag ctgctctact accgcaaccc gatggggctg
     2101 cccgatacct cgccgacccc aaagaaggac cccatgggca tggactacat cgcagtctat
     2161 gaggatgaag gaagcaccga aagcagtgaa ccggtttccg gcaaccagct caggatcagc
     2221 accgacaagg ttcaaaaaat gggggtacgt acggaagccg cccaactgcg agatctgggc
     2281 aggatagtcc gcgcagccgg gcgaatcgag ccggatgagc gccgcgtctt cacgatctcg
     2341 cccaaattcg agggttacgt cgaacgcctc tacgtcaata ttaccggcca gcccgttggc
     2401 aagggacagg caatgttcga ggtctacagc ccggaactgg tttcggcgca gcgtgaatat
     2461 gccattgccg cgcagggtgt cgaagcgttg aaggacggtg acaattacgc tcagtccggg
     2521 atgaaggaat tggccgaatc gagcctcacg cgcctgaaaa actgggacat ctcggaagat
     2581 caggtcagga ccctggtcaa ctccggcaaa tcgaagcaca ctctggtatt ccgctctccg
     2641 gtgaatggga tcgtcaccga aaagaaggcg atgcagggca tgcgcttcat gcctggcgaa
     2701 gcgctctacc aggtagccga tctgtcttcc gtctgggtta tcgccgatgt tttcgaacag
     2761 gacatcggcc ttgtcaagac cggcgccaag gccaaggtga cgatcagtgc ttatccggaa
     2821 atgatgttcg agggaacgat cacctacgtc tacccgacgc tgaacgccca gacccgcacg
     2881 gttccgattc gagtggagct ggccaatcct ggcctgctgc ttaagccttc catgttcgcg
     2941 caggtggagt tgccggtatc ggccaagggt acggtggtga cggtgccggt ttccgctgtg
     3001 atcgacagcg gtacgcgcca gatagtgctg atccggcaag gcgaaggccg cttcgagccg
     3061 cgtgaggtca agctcggggc acgcagcgac aatcatgccg aagtgctgga gggggtcaag
     3121 gatggtgaac aggttgtggt cgccgccaac ttcctgatcg atgccgaaag caacctcaag
     3181 gcggcagtcg gcggtttcgg ccaagccacc catgacacag cccccgtgga aggaccagcg
     3241 acagcgcccg cacatccgac caccagagga gccagccacc atgccgaagg aacggtaggc
     3301 agcatcaatg cgcaagccgg cacgatcaat ctcagccacg gcccggtcgc cagcctcaaa
     3361 tggccagcca tgacgatgga gttcaaggcc gccaacgaat cgctgctgca aggtctgaaa
     3421 ccagggagca aggtcgacgt cgaattcgtc gagcggcagc ccggcgaatg ggtgatcacg
     3481 gccatcaaac cacttccggc aagcaaggcg gctggcatgc cgaccgccgg tgcgaacccg
     3541 cacgccgggc attgaccgac agccacggag attcgcgcca tgctggccaa cattatcgaa
     3601 tggtcgggca agaaccgttt tctggtcctg ctcgccaccc tgttcatcac catcggtggc
     3661 atctttgccg tactgcgcac gccgatcgat gccttgcccg acctctcgga cgtccaggtc
     3721 atcgtctaca ccgagtatcc cggacaggcg ccgcaggtgg tcgaggatca ggtaacctat
     3781 ccgctcacca cggccatgtt gtcggtgccg aaatc
//
LOCUS       DAORPM010000239         3799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7709_length_3799_cov_8.182959, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000239 DAORPM010000000
VERSION     DAORPM010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7709_length_3799_cov_8.182959"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..687)
                     /gene="pgsA"
                     /locus_tag="PLD53_10320"
     CDS             complement(109..687)
                     /gene="pgsA"
                     /locus_tag="PLD53_10320"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRC61353.1"
                     /translation="MPLNIPILLTWLRIVAIPLMITVYFVPASWAEPHERDLVATLIF
                     MAAAVTDWADGYLARKLNQTSAFGAFLDPVADKLMVAAALIILVQLGRADAIVATIII
                     GREITISALREWMAKIGASKNVAVSMLGKIKTAAQMLAIPALLYYAPWFGLDLRLVGN
                     WLIWIAALLTLWSMGYYLRMAWPEIAKRSAEK"
     gene            complement(691..2502)
                     /gene="uvrC"
                     /locus_tag="PLD53_10325"
     CDS             complement(691..2502)
                     /gene="uvrC"
                     /locus_tag="PLD53_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRC61354.1"
                     /translation="MSFDAKAFLASLTELPGVYRMLDAGGAVLYVGKAKNLKKRVSSY
                     FRENVSSPRIAHMVSQIAAIETTSTRTEAEALLLENNLIKSLSPRYNILFRDDKSYPY
                     IVVTKGEFPRLGFYRGNPDRKADYFGPYPSSWAVRDSIHLLQKMFRLRTCEDTVFSNR
                     SRPCLLYQIKRCSGPCVELISPQDYAADVQLASMFLLGKQHEVNKRLTLSMEEASSRL
                     AFEQAAVFRDQIQSLRQVQEKQFVSSSKGEDVDIVVALQAAGQLCVNIAMVRGGRHLG
                     DRPLFPSNAGESSASDAIAAFIHQHYAAHPAPARLLVLPFPTEEEKGEAEISLAQLAG
                     RHVPILEAYSVVNKAWVDMALQNAQLAILARNQATAQQEQRLAALQEALQLPDAIARI
                     ECFDISHTQGEATVASCVVYQGNGMKKADYRRFNIRDIQPGDDYAAMRQAVSRRYDSI
                     AAGEGIAPDLILIDGGKGQVSSAYSALSDLGLAHLSMLGVAKGEGRKPGLESLIFPDG
                     REPLQLPPNHPALHLIQEIRDEAHRFAITGHRAQRSKARKTSKLDSLPGIGPARRKAL
                     VARFGGLAGVLAATADQLAEVTGISQEMADKIYSALH"
     gene            complement(2515..3060)
                     /locus_tag="PLD53_10330"
     CDS             complement(2515..3060)
                     /locus_tag="PLD53_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRC61355.1"
                     /translation="MPAFGSLDSELLVVGLGPGEKGANRTGRPFTGDVAGELLYPALH
                     RFGFASATEALGPDSWANPAMQLSNCRITNAVRCLPPANKPVTAEIRQCNSHLKAELS
                     AMPRLKVIVALGAIAHQALLWAYDLKLKQHVFGHNVTHSLPDGRLLVDSYHCSGYNWR
                     TGRLSRESFEAVFAGVKAHLG"
     gene            3178..>3799
                     /gene="earP"
                     /locus_tag="PLD53_10335"
     CDS             3178..>3799
                     /gene="earP"
                     /locus_tag="PLD53_10335"
                     /inference="COORDINATES: protein motif:HMM:NF021598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HRC61356.1"
                     /translation="MASMQLHWDIFCRVIDNYGDIGVCWRLARQLANEHGKQVRLWLD
                     DLNSLQPLCPAIDVFQAEQHCLGVEIIHWTEHAAVDRVGEVIIEAFACELPDRYLKVM
                     SQTTPKPCWINLEYLTAETWAEDCHGMASPHPSLPLIKYFFFPGFSSSSGGVLRERGL
                     MAERDKVLAPLSRDDALDISLFCYDTAPVGELLEVLTQSTTPVRLHV"
BASE COUNT          849 a         1016 c         1068 g          866 t
ORIGIN      
        1 aactctcgac ctcaaccttg gcaaggttgc gctctaccaa ctgagctact gccgcgtcaa
       61 acagagcgcg cattatagac gtaccaaatg atatgtcaac atgtccgatc atttttctgc
      121 gctgcgcttg gcaatttccg gccaggccat gcgcaggtag tagcccatgg accaaagtgt
      181 caacaaggca gcgatccaaa tcaaccagtt acctaccaaa cgcagatcca acccaaacca
      241 cggggcgtaa tagagtaaag caggaatggc gagcatttgt gcagcggtct tgattttgcc
      301 aagcatggaa actgcaacat tcttggatgc ccctattttg gccatccact cccgaagtgc
      361 agaaatggta atttctctgc cgataataat agttgcgacg attgcgtcag ctctgccgag
      421 ttgcaccagg atgatgagtg cagctgcgac catcagcttg tcagcaaccg ggtcgagaaa
      481 agcgccgaag gcggaggttt ggttcagttt tctggccaga taaccatcgg cccagtcagt
      541 cactgcagcc gccataaaga tcagtgttgc caccaagtcg cgttcatggg gttctgccca
      601 gcttgccggt acgaaataaa ccgtgatcat caacggaatt gcgacgatac ggagccaagt
      661 tagcaggatg gggatattga gaggcatgtg tcagtgtagt gcagaataaa ttttatcggc
      721 catttcctgg ctgatgcctg tgacttcggc cagttgatcg gccgtggcag caagaacacc
      781 ggctaggcca ccaaatcggg cgaccagtgc ttttcttcgc gctggaccaa tgcccggcag
      841 actatcgagc tttgacgtct ttctggcctt gctacgttgg gcgcggtgac cggtgatggc
      901 aaaacgatgt gcttcgtcac gaatttcctg gattagatgc aatgctggat gatttggcgg
      961 caattgtagc ggttcacgac catctggaaa aattagcgac tccaggccag gcttgcgccc
     1021 ctctccctta gcaacgccca gcattgataa atgcgccaat ccaagatcag agagtgcgct
     1081 gtaagccgag ctcacctgcc ctttgccacc gtcgatcaaa atcagatcgg gtgcaatccc
     1141 ctccccggct gcaatactgt catagcgccg ggaaactgct tgacgcatgg ccgcgtagtc
     1201 gtcaccgggt tgaatatcgc gaatattaaa gcgccgataa tccgcctttt tcatcccgtt
     1261 tccctggtag accacgcagg atgcaacggt cgcttcgccc tgagtgtggc ttatgtcaaa
     1321 gcattcgatc cgagcgatcg catcaggcaa ctgtaatgct tcttgcaaag ccgccaagcg
     1381 ctgctcttgc tgagcagtcg cctgattgcg ggccagaatg gcgagttgag cgttttgcag
     1441 cgccatgtct acccatgctt tattgacgac tgagtaggct tccagaatgg gcacatgccg
     1501 tcctgcaagc tgagccaaag aaatttcggc ttcacccttt tcttcttcgg tcggaaatgg
     1561 cagcactaga agcctggctg gtgctggatg tgcggcgtaa tgttgatgaa taaaggcggc
     1621 aattgcatcg ctagccgacg attcaccggc attgctcgga aacagtggtc gatccccgag
     1681 gtgtcggcct ccccgaacca tggcaatatt gacgcacaac tgaccagccg cctgcagcgc
     1741 aacgacgata tcgacatctt cgcctttgct gctggagacg aattgctttt cctgcacttg
     1801 gcgcagtgac tggatttggt cacgaaatac agcggcttgc tcaaaagcca aacgactgga
     1861 ggcctcttcc atggacagcg tcaatcgctt attgacttcg tgttgcttgc cgagcaggaa
     1921 catcgaggca agctgaacgt cggcagcgta gtcttggggc gatatgagct cgacgcaggg
     1981 gccgctgcag cgtttgatct gataaagcag gcaggggcgc gagcggttgg aaaatacagt
     2041 gtcttcgcag gtgcgcaggc gaaacatttt ctgcaacagg tgaatactgt cgcggacagc
     2101 ccaggacgag gggtacggac cgaaatagtc cgccttgcga tccgggttgc cccggtaaaa
     2161 gcccaggcga ggaaactcgc ccttggtaac aacgatgtag ggataggatt tatcgtcgcg
     2221 aaacaggatg ttgtaacgcg gggatagcga tttgatcaga ttgttttcaa gcagcagtgc
     2281 ctctgcctcg gttcgcgtcg aggtggtttc gatcgctgcg atctggctga ccatatgggc
     2341 aatgcgcggg ctggagacgt tttcgcgaaa gtaggacgaa acccgttttt tcagattttt
     2401 tgctttgccg acgtaaagga cagccccccc ggcatcaagc attctgtaca cgccgggcaa
     2461 ttcggtaagc gaggctaaaa aagccttggc atcaaaactc attacggcaa ccgtttagcc
     2521 gagatgcgct ttgacgccgg caaatacagc ttcaaagctt tcccgagaca gccgcccggt
     2581 tcgccagttg tagccactgc agtgatagct gtcgaccagc aaacgtccgt ccggcaagct
     2641 gtgcgtgaca ttgtgaccaa agacatgctg tttaagttta aggtcataag cccagagcaa
     2701 agcctgatgt gcgatcgcgc ccagcgcgac aatcactttc aaacgcggca ttgcgctcag
     2761 ttcggccttg agatgactat tgcattggcg aatttcagct gttaccggct tgttggcagg
     2821 cggcaggcag cgcacggcat tggtgatccg gcagttgctc agttgcatgg ccggattggc
     2881 ccaactatcc ggccccaagg cttcggttgc actggcgaaa ccaaaacgat gaagcgccgg
     2941 gtagagcaac tccccagcca cgtctcccgt gaatgggcgg ccagttctat tcgccccttt
     3001 ttcaccgggg ccgaggccga cgacgagcag ttctgaatcc agggagccga aggcaggcac
     3061 gggcaatgcg tgccagttag ggtctcgctg gcgaatggtc gcgaggtgcg aagccaggcg
     3121 cgggcaggca gtgcactctt ttgggtcttg aaataaaggc atggtggcga tgttagcatg
     3181 gcgtccatgc agcttcattg ggacatcttt tgccgtgtga ttgataacta cggcgacatc
     3241 ggcgtttgct ggcggctcgc ccggcaattg gcgaacgagc acggcaaaca ggttcgtctt
     3301 tggcttgatg atttgaatag cttgcagccg ctatgtcctg ctatagatgt tttccaggct
     3361 gaacagcatt gcctgggcgt ggaaattatt cactggactg agcatgctgc ggtcgaccgt
     3421 gtgggcgagg taattatcga agccttcgcg tgcgagcttc ccgatcgcta tctgaaagtg
     3481 atgagccaga ctacaccgaa gccctgctgg atcaatctcg aatacctgac cgctgaaacc
     3541 tgggcggaag actgccacgg catggcatca ccccaccctt ctcttcccct gatcaaatac
     3601 ttttttttcc cggggttctc ctcttcaagt ggtggcgtct tgcgggaacg tggtctgatg
     3661 gccgagcgcg acaaggtgct ggcccccctt tctcgtgatg acgcgctcga tatcagtctg
     3721 ttctgttacg acacggcgcc ggtaggcgaa cttctggaag tgttgaccca atcgacgact
     3781 ccggttcggc tgcatgtcg
//
LOCUS       DAORPM010000240         3798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7713_length_3798_cov_8.502271, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000240 DAORPM010000000
VERSION     DAORPM010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7713_length_3798_cov_8.502271"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1193
                     /locus_tag="PLD53_10340"
     CDS             24..1193
                     /locus_tag="PLD53_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRC61357.1"
                     /translation="MTRKIQTLNAISKKGLSRLPEDYVVSNDGTEPDAILVRSQVMHE
                     LPIPSSVLAIGRAGAGTNNIPIKTMSERGVVVFNAPGANANAVKELVIAGMLIGTRNL
                     YPALNFVNGLKGDDEALHKQVEAGKKHYVGHELSGSTLGIIGLGAIGSMLAESAIQLG
                     MNVIGFDPDITVDAAWRLPAHVKKAASVDDLVKRADFVSIHVPFLPATKDLINAERVK
                     LMKKGCILLNFARDGIVNEQAVLDGLNDGKLHAYVCDFASNLLRQHPRFVALPHLGAS
                     TEEAEENCAIMVADQVADYLENGNIFNAVNFPNIAMLRESGYRLAIANANVPNMLGQI
                     STTLADAGLNIHNMVNKSRGDLAFTLVDVETAVPQAVIDQLNAISGVLRVRYLPS"
     gene            1226..1702
                     /locus_tag="PLD53_10345"
     CDS             1226..1702
                     /locus_tag="PLD53_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRC61358.1"
                     /translation="MSVKPIPDGYHSVTPYLSIKGAAEAIEFYKQALGANELFRLVMP
                     SGDIGHAEIMIGDSHIMMADPCEEFNFRSPKVLGGSSVGLHVYVEDVDKLFAQAVAAG
                     AKVVKPVQDQFYGDRGGTLEDPFGHVWFLATHKEDLAPEEINRRAEALFKQGNGQS"
     gene            complement(1819..2670)
                     /gene="rpoH"
                     /locus_tag="PLD53_10350"
     CDS             complement(1819..2670)
                     /gene="rpoH"
                     /locus_tag="PLD53_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HRC61359.1"
                     /translation="MTQALVFPSISSVGNIDAYIQATRQFPLLTQEEEVRLATRFRDE
                     EDLEAARQLVLSHLRLVVSIARGYLGYGLPHADLIQEGNIGLMKAVRRFDPAHGVRLV
                     SFAMHWIKAEIHEYVLRNWRMVKLATTKSQRKLFFNLRSMKPGSESLTSPQVANVAHQ
                     LSVKPEEVIEMETRLSGRDLALESGRDDADDFAPIAYLEDNRSEPTQVLESRARDHLQ
                     GEGLQAALSDLDERSRRIVEERWLSDDPATLHELADEFGVSAERIRQIETKAMQKMRT
                     SLSPLVA"
     gene            3097..>3798
                     /locus_tag="PLD53_10355"
     CDS             3097..>3798
                     /locus_tag="PLD53_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRC61360.1"
                     /translation="MINPGAPAVVCQGRSLTYGELAERAARLAACLTHRLDWQRLDGQ
                     PPCVGILASRGIDACVALLGACWAGATYVPISLKQPEERILTLLAQCGLSAIIADDEG
                     AKLLSERVLAACPQVVIHAGQTSLTPRDGAVEIIEMATLPTVIPEEPAPMAAPDTAYI
                     IFTSGTTGIPKGVMISACSARHYVTMIAKWLELRASDRALETCELSFDFSVHNMFATW
                     EAGAALYILPATTVMN"
BASE COUNT          864 a         1093 c         1053 g          788 t
ORIGIN      
        1 agcaaataga cgaggaaaca cgcatgaccc gcaaaattca gacactcaat gccatctcga
       61 agaaggggct ttcccggctg cccgaagact atgtcgtcag caatgacggc accgaaccgg
      121 acgccattct ggtgcgctcc caggtaatgc acgaactgcc cattccgtcc agcgtcctcg
      181 ccatcggccg cgcaggtgcc ggtaccaaca atattccgat caagaccatg agcgaacgcg
      241 gcgtggtcgt attcaacgcg ccgggcgcca atgccaatgc agtcaaggaa ctagtcatcg
      301 ccggcatgct gatcggaacg cgcaacttgt acccggcgct caattttgtg aacggtttga
      361 agggggacga cgaggcgctg cacaagcagg tcgaggcggg caagaagcat tatgtcgggc
      421 atgagctgag cggcagtacg ctgggcatca ttggtctggg tgcgatcggc tcgatgcttg
      481 cggaatcggc catccagctc ggcatgaacg ttatcggctt cgatccggat atcaccgtgg
      541 acgcggcctg gcgcctgccc gcgcatgtca aaaaggcagc cagcgtggat gatctggtaa
      601 agcgtgcgga tttcgtcagc atacacgttc cctttcttcc cgcgaccaag gatttgatca
      661 atgccgagcg cgtcaagctg atgaagaagg gctgcattct gctcaacttt gcccgtgatg
      721 gcatcgtcaa tgagcaggcc gtactcgatg gcctcaacga cggcaaactg catgcctacg
      781 tttgtgattt cgcgagcaac ctgctacgcc agcatccgcg cttcgtcgca ctgccgcacc
      841 ttggcgcctc gaccgaggaa gccgaagaaa actgcgccat catggtcgcc gaccaagtgg
      901 ccgactatct ggaaaacggc aacattttca atgccgtgaa cttccccaac atcgccatgc
      961 ttcgcgaatc cggctatcgg ctggccatcg ccaacgccaa tgtgccgaac atgctgggac
     1021 agatttcgac gacactcgcc gacgccgggc tgaacatcca caacatggtc aataagtcgc
     1081 gtggcgatct cgctttcacg ctggtggatg tcgaaacggc tgtgccgcaa gccgtcatcg
     1141 atcaattgaa cgcgatcagc ggcgtattgc gtgtgcgtta cctgccgtcc tgaaatgcgt
     1201 catcaaggcg agaaacgaga acaacatgtc tgtcaaaccc atacccgatg gctaccacag
     1261 cgtcacgcct tatctgagca ttaaaggtgc cgccgaggcc atcgagtttt acaagcaggc
     1321 cctgggggcg aatgagttgt ttcgtctggt tatgcccagt ggcgatatcg gacatgcgga
     1381 aataatgatc ggcgattcgc acatcatgat ggccgacccg tgtgaagagt tcaatttccg
     1441 tagccccaag gtgctgggtg gctcgtcggt tgggttgcac gtgtatgtcg aggatgtaga
     1501 caaacttttt gcccaggcgg tcgcagcagg tgcgaaggtc gtaaagcccg tgcaagacca
     1561 attctatggc gaccgtggcg gcacgctgga agacccgttc ggacacgtct ggtttctggc
     1621 aacgcataag gaagaccttg cgccagagga aatcaacaga cgtgccgaag cgctcttcaa
     1681 gcagggcaat gggcaaagct aggggcagaa gtagcgcccc gtcgttgcgc agcgtcatgt
     1741 ctccagaata gaaaaagccg ttcagtgatc actgaacggc ttctccggag gcttccagcc
     1801 caaaacggct tatcagcttc aggcaaccag cggggaaagg ctggtacgca tcttttgcat
     1861 ggctttggtt tcaatctggc gaatgcgctc ggcggaaaca ccgaattcat cagccaactc
     1921 atgcaatgtt gcgggatcat cgctaagcca gcgttcctca acgatccggc gactacgctc
     1981 gtcgaggtcg gaaagcgctg cctgcaatcc ctcgccctga agatgatcgc gagcgcgcga
     2041 ttcaagcacc tgtgtcggct cgctacgatt gtcctcaagg taagcaatcg gtgcaaaatc
     2101 atccgcgtcg tcccgacccg attcaagcgc cagatcacga cccgataggc gggtttccat
     2161 ctcgatgact tcttccggct tcacgctgag ctgatgcgca acattggcca cctgcggaga
     2221 tgtcaaagac tcacttcccg gcttcatgct gcgcagattg aaaaacagct tgcgttgcga
     2281 cttggtcgtg gccaatttga ccatacgcca gtttctcagc acgtattcgt ggatttcggc
     2341 cttgatccag tgcatcgcga aggacacaag acgcacacca tgggccggat caaagcgtct
     2401 gacggccttc atcagaccga tgtttccttc ctgaatcaaa tcggcatgtg gcaggccgta
     2461 gccgaggtac ccacgggcaa tcgacactac cagacgcagg tgagagagga ccaactggcg
     2521 cgccgcctca agatcctcct cgtcgcggaa tcgcgtcgcc agccgaacct cctcctcctg
     2581 cgtcagaagc gggaactgcc gcgttgcctg aatgtaggcg tcaatatttc caaccgagga
     2641 gatcgacgga aaaacaagag cttgcgtcat ttcaacctct cctttctatt gttcacgaga
     2701 tcattttagc actcaatagc tgagagtgct aagagggcga aaagtttctt gtatcggttt
     2761 gtaattgagg aaatccagca aagaacgttc gatcctgtaa aggcgacacg agtgttgttt
     2821 tgattgccgc gtcgcctcga tgggccgttg tcaggttgaa tacgccgccc aggcagaaag
     2881 gtcatgagca atcctcagta acaggtaaaa ttgcgccctt tttggccgca gaaggagccg
     2941 atgccctcaa attccccagg ccgcccgtca atcaaaattg cacttcggat tcgaactcac
     3001 gaatccgtta tcgatccgca gcagacgata taaccctctt tcgaaaatat gatgcttaag
     3061 aactataacc tagcatgtgc catcaatcat cactctatga tcaatcctgg agccccagcg
     3121 gtagtatgcc aaggccgttc gctgacctac ggcgaactgg ccgagcgtgc agcccgtctt
     3181 gccgcctgtc tgacccacag acttgattgg cagcgcctgg acgggcaacc accctgcgtc
     3241 gggattctcg cttcccgtgg aatcgatgct tgcgttgcct tgctcggcgc gtgctgggca
     3301 ggggcgacct acgttccgat cagtctgaaa cagccggaag agcgaatctt gaccttgctc
     3361 gcccaatgcg gattatcggc aatcattgcc gatgacgagg gggcaaagct gctcagcgag
     3421 cgcgtgctcg ccgcctgtcc gcaggtcgtg attcacgccg ggcaaacatc gcttacgccg
     3481 cgggatggcg ccgtagaaat aatcgagatg gcgacgcttc caacggtgat tcctgaggaa
     3541 ccggcgccca tggcggcgcc tgacacggcg tacatcattt tcacctcggg aacgaccggc
     3601 atcccgaagg gcgttatgat ttctgcgtgc tctgcacgcc attacgtgac gatgatcgct
     3661 aagtggcttg agttgcgagc cagtgaccga gccttggaaa cctgcgaact cagcttcgat
     3721 ttctcggtgc acaacatgtt cgcaacgtgg gaagccggag ccgccctgta tatcctgccg
     3781 gccactacgg tgatgaac
//
LOCUS       DAORPM010000241         3752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7855_length_3752_cov_12.415742, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000241 DAORPM010000000
VERSION     DAORPM010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7855_length_3752_cov_12.415742"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1167)
                     /locus_tag="PLD53_10360"
     CDS             complement(13..1167)
                     /locus_tag="PLD53_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61361.1"
                     /translation="MQQAVERLTSTALPVSEGFLDTVAVAMLGCCGVLIESGDLSPVR
                     VLAPSLPIASELRTAMLRAAPRPLLLPRFDTLTHWVNSTPFATIPEPLPDSERVVLLH
                     EALRGRGWFDEAALWGIALEMAGLFDELSAATVTLPDDGSKLAEQLERAYSLRVSVPL
                     AFEARVVHELWCALAAAGQPDSSAAYGLRLAALIKQVEQVDARTCRLLVLLDAAPEES
                     LAPAELDFFLRYGHVHSLSMFHPAPRFACTSPLMATLAAAWPEAPALLTQKATPLFER
                     AAELACMQSESPLAERLQLVPARGRELEAQAAVAQIGAWLSAGLRRIVLIAQDRLTAR
                     RVRALLEREGVLVSDETGWKLSTSRAAAGVDALIETAASGAYYRDLLDLC"
     gene            complement(1204..1527)
                     /locus_tag="PLD53_10365"
     CDS             complement(1204..1527)
                     /locus_tag="PLD53_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRC61362.1"
                     /translation="MTYVVTENCIKCKYTDCVDVCPVDCFHEGPNFLTIDPEECIDCT
                     LCVAECPAEAIFAEEDVPEDQHKFIALNAELAKNWPVISERREPPADADDWNGTPDKL
                     EYLER"
     gene            1683..2009
                     /gene="trxA"
                     /locus_tag="PLD53_10370"
     CDS             1683..2009
                     /gene="trxA"
                     /locus_tag="PLD53_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HRC61363.1"
                     /translation="MSEHIHHVTDDTFAAEVLQAQQPVLVDYWAEWCGPCKMIAPILD
                     EISDQYSGRLKVAKLNIDDNQNTPASYGVRGIPTLMLFKNGNIEATKVGALSKSQLCT
                     FIDSHL"
     gene            2232..3488
                     /gene="rho"
                     /locus_tag="PLD53_10375"
     CDS             2232..3488
                     /gene="rho"
                     /locus_tag="PLD53_10375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRC61364.1"
                     /translation="MHLSELKALHVSQLLDMAVTNTIEGANRLRKHELIFALLKNQAK
                     KGETIFGDGTMETLQDGFGFLRSPDTSYLASTDDIYVSPSQIRRFNLHTGDTIEGEIR
                     TPKDGERYFALVKVDKINGEAPEASKNKILFENLTPLHPTEHMKLERDIRGEENTTSR
                     IIDIIAPIGKGQRGLLVSSPKSGKTVMMQHIAHAITTNHPDVTVIVLLIDERPEEVTE
                     MTRSVRGEVVASTFDEPATRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VQPASGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNLEIHLDRRMAEKRVYPAINVNRSGTRREELLLMPDVLQKMWVLRKLLYNMDD
                     LEAMEFLLDKIKATKNNGEFFDSMRR"
     gene            3662..>3752
                     /gene="rpmE"
                     /locus_tag="PLD53_10380"
     CDS             3662..>3752
                     /gene="rpmE"
                     /locus_tag="PLD53_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRC61365.1"
                     /translation="MKSDIHPDYNEIEVTCSCGSVFKTQSTMKK"
BASE COUNT          894 a         1129 c         1019 g          710 t
ORIGIN      
        1 gatgtatggc gattagcata ggtcaagcag gtcgcgatag taggcgccgc tggcagccgt
       61 ttcgatcagt gcatccacgc ctgccgcagc tcgggaggtg gaaagcttcc agccagtttc
      121 gtcgctgact agcactccct cgcgttcaag gagcgcacgg actcgccgcg ccgtcagacg
      181 atcctgggct atcaggacaa tacggcgcaa tccggcactc agccatgcgc cgatttgcgc
      241 taccgccgcc tgtgcctcca gctcccggcc tctggccgga acaagttgca agcgctctgc
      301 cagtggactc tccgattgca tgcacgccag ctcggcggca cgctcgaata gtggggtggc
      361 tttttgcgta agcaaagcgg gagcctccgg ccaagctgcg gcaagcgttg ccatcagagg
      421 cgaagtgcat gcgaagcgcg gcgccggatg aaacatggac agggagtgta catgcccata
      481 gcgtaggaag aaatcgagct cggccggcgc aagcgactcc tccggtgcgg cgtcaagtag
      541 caccaatagt cgacacgtcc gtgcgtcgac ctgctcgacc tgtttgatca gcgcggccag
      601 gcgcaagccg taagcggccg aggaatcggg ctgcccggca gccgccagcg cacaccagag
      661 ttcatgcacg acgcgcgctt cgaaagccaa tggaaccgaa acacgaagcg aataggcgcg
      721 ttcaagctgt tcggccagtt tgcttccatc atccggcaga gttacggtgg ctgccgacaa
      781 ctcgtcgaaa agtccggcca tctccaacgc aataccccat agtgccgcct cgtcaaacca
      841 gccgcgcccg cgcagagctt catgcagcag cacgacgcgc tcgctatccg gcaacggctc
      901 aggaatcgtg gcgaagggag tggagttgac ccagtgcgtc agtgtgtcga agcgcggcag
      961 aagcagtggc cttggggccg ctcgcagcat ggcggtgcgc agctcggacg cgattggcaa
     1021 cgagggcgcc aggactcgta ccggcgaaag atcgccagac tcgatcagca cgccgcaaca
     1081 gccgagcatg gcgacagcga cagtatcgag gaatccctcg gagacgggca aggctgtcga
     1141 ggtcagccgc tcaacagcct gctgcatcgg aaccggtaag aaagaaatac cggaagtact
     1201 ggctcagcgt tcgaggtact cgagcttgtc cggcgtaccg ttccaatcgt cggcgtcggc
     1261 cggcggctcg cgacgttcgg agatgaccgg ccagttcttg gccaactcgg cgttcagagc
     1321 aatgaacttg tgctggtcct ccggcacgtc ttcctcggca aaaatggcct cggccgggca
     1381 ctcggccacg catagcgtgc aatcaatgca ctcctccgga tcgatcgtca gaaaattggg
     1441 gccttcgtga aagcagtcga ctgggcaaac atccacgcag tccgtgtatt tacacttgat
     1501 acagttttca gtcacaacgt aagtcatatt tcctgctctt caaagttagc gcgcaaaaat
     1561 tatagcgtat ttgcctgtcc gcttcccttg ccgcttgttt ttagctagga tagccccatc
     1621 tgccgtgtct cgcgcccagg caggcacctt ccccaaatct ccgttaccaa aaaggcttta
     1681 tgatgagcga acacatccat cacgtcaccg atgacacctt tgcagccgaa gttctgcagg
     1741 cgcagcaacc cgtactggtc gactactggg cagaatggtg cggcccatgc aaaatgattg
     1801 ccccgatcct cgacgaaatc tctgatcagt actcgggtcg cctgaaggtt gcaaaactga
     1861 acatcgacga caaccagaat accccggcta gttacggtgt gcgcggcatt cccaccctga
     1921 tgctgttcaa gaacggaaac atcgaggcca ccaaggttgg cgcactgtcc aaatcgcaac
     1981 tctgcacttt cattgacagc catctgtaaa atcgataccc tctgcccgaa ggttcaaaag
     2041 gtgcggcgcc tgagaacaga aataacacgg tccgcataga accttcaagc tcgatctcca
     2101 gtcgattttt gtagctcggc aacagccccg tcgcacctga tcgtaacacc cccgccgata
     2161 cccaaagccc ccccgcacgc gcatcattcg tgatagcgca tcatgccctc ccaattttca
     2221 attgtctgct catgcaccta tccgaactca aagccctaca tgtaagccag ttactcgaca
     2281 tggccgtcac caacacgatc gaaggcgcca atcgacttcg aaagcacgag ctgatttttg
     2341 cgttgctcaa gaatcaagca aaaaagggag aaacaatttt cggcgacggc accatggaga
     2401 ccctgcagga tggtttcgga ttcctgcgct cccccgacac ctcgtaccta gccagcacgg
     2461 atgacattta tgtaagtccg agccagatac gccgtttcaa cttgcatacg ggcgatacga
     2521 tagagggtga gatccgcacg cccaaggatg gcgaacgcta ttttgcgctg gtaaaagtcg
     2581 acaagatcaa cggcgaggct ccggaggctt caaaaaacaa gatccttttt gagaatctca
     2641 cgcccttgca tccgaccgag catatgaagc tcgaacgcga tattcgtggc gaagaaaaca
     2701 cgaccagtcg catcatcgac atcatcgcgc cgatcggcaa aggccagcgg ggcctgctgg
     2761 tatcgagccc gaaaagcggc aagaccgtca tgatgcagca tatcgcgcac gccattacaa
     2821 ccaatcatcc cgacgtgacg gtcatcgttc tgctgatcga cgaacgccct gaagaagtga
     2881 ccgaaatgac gcgctcggtg cgcggcgagg tggtcgcctc aacctttgac gaaccggcga
     2941 ctcgccacgt tcaggttgcc gaaatggtca ttgaaaaagc taaacgtctg gtcgagcata
     3001 agaaggatgt tgtcatcctt cttgattcga tcacccgtct ggcccgcgcc tacaatacgg
     3061 tgcaacccgc ttcgggcaag gtactgaccg gcggtgtcga tgcaaacgcc ctgcagaagc
     3121 ccaagcgctt cttcggtgcc gcgcgcaata tcgaggaggg cggctcgctg accatcatcg
     3181 ccaccgcgct gatcgatacg ggcagccgca tggacgacgt gatctacgaa gagttcaagg
     3241 gcaccggcaa cctggaaatc cacctggatc ggcgaatggc ggagaagcgt gtatacccgg
     3301 cgatcaacgt caatcgttcc ggcacccgcc gtgaagaact tctgctgatg cccgacgttc
     3361 tgcaaaagat gtgggtacta cgcaagctgc tctacaacat ggatgatctg gaggcgatgg
     3421 aattccttct cgacaagatc aaggcaacga aaaacaatgg ggaattcttc gactcaatgc
     3481 gccgctaacc ccttcctacc cccttcctga ttgcaaattc acgaagaatc aaggataatg
     3541 cgccgttctc cggagccggt cccgaagcag caaacgcgaa atgtcaggcg cgattcacta
     3601 ggcgcgatgt gcgcgtagtt tcctcgaccc ggctagattt gacttacagg aatttctcaa
     3661 gatgaaatcc gatatccacc ccgactacaa cgaaatcgaa gtcacctgct cctgcggcag
     3721 cgtgttcaag acccagtcga cgatgaagaa gg
//
LOCUS       DAORPM010000242         3752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7856_length_3752_cov_8.616446, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000242 DAORPM010000000
VERSION     DAORPM010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7856_length_3752_cov_8.616446"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..1744)
                     /locus_tag="PLD53_10385"
     CDS             complement(263..1744)
                     /locus_tag="PLD53_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HRC61366.1"
                     /translation="MTDFLGSLFPVRILAQGLRGAAFLRLRIEDGEASFSRLVGLIAV
                     TIALQFAMDFYSVGPQGELSPYGLPGLLFKVPMILIAAWALSLLAKRPEATLMLAVAI
                     SAIAIPISVLGDLLLWLLGSPLIARNFHLQDSYYLLYAHLAPAWLGLATAVAATRLFD
                     LSGKKVALSVLSGVVLIWFPLALAGGYRTIWAVPFDAESAARERAQQNALLEERAFYS
                     QPRILEHALASLDRSQPDRINLYFVGVAGYSEQDVFMKEVHYVSNFFKARFGTTGRSI
                     TLINNPKTVLESPAASVTSLRLALNQVGKKMDTDKDILFLYLTSHGSREHRLSLSFGS
                     MRFDVLDPGVLRRILDDSGIKRRVVVVSSCYSGGFVEPLKNENTLVISASAPDKTSHG
                     CSNEADFTFFGKAYFEDGLRKTDSFIEAFNIASPLIAERDAKEGYEAALPMMNVGTGI
                     RAALNQYTAEQKQLALQRSAPTRAEADPEAQVKQSPISCASCQ"
     gene            complement(1974..2888)
                     /locus_tag="PLD53_10390"
     CDS             complement(1974..2888)
                     /locus_tag="PLD53_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRC61367.1"
                     /translation="MKTLAFNIAAVERDTGLSKDVLRMWERRYGFPEPERDGNGERLY
                     PETQVERLRLIKRLMDQGHRPGKLMAASPEELAQLAPRRPASRQMGAEIDGEDLGELL
                     ILIKQHDAAGYQQAMQQRLARQGFQSFVQDTVAPLTRRVGEAWEDGSFEVFEEHLFTE
                     LTKRLLRQAIAALPGGVRSPRILLTSVPDEQHVLGLLMAEGLFVLEGAECIPLGTQMP
                     LLEIGHAAVAHQANIVALSFSIAFRSRQIPGLLQQLRQILPMTIELWVGGRGVCGLAP
                     IAGIRLLSGLDSTISALADWRAENMPRN"
     gene            2996..>3752
                     /gene="folE2"
                     /locus_tag="PLD53_10395"
     CDS             2996..>3752
                     /gene="folE2"
                     /locus_tag="PLD53_10395"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRC61368.1"
                     /translation="MNAPEHFFLPDVQALTDSRSLAIQQVGVRGLRYPLQVETAGGET
                     LATVATLGMTVGLPPEVKGTHMSRFVELLEGCSGAVTQGSLFHLLDDMLCRLDAQSGR
                     IELAFPYFIRKTAPVSGVTSLLDYDATMIIEQREGERASLTLRVVAPVTSLCPCSKGI
                     SDYGAHNQRSHITLEAKLRRPMAIEELVRLAEEEASCEVFGLLKRPDEKWVTERAYDN
                     PKFVEDLVRDIALRLMEEPRIAEWKVASENFESI"
BASE COUNT          786 a         1088 c         1120 g          758 t
ORIGIN      
        1 attcttggtg agatcacccg tttggtacgg actgcccatc ttcgcggtca catcagcctg
       61 agtcgacttt cctaacacaa gcactccttc tgcagggcga acaaaatctg tccccgcaca
      121 agcagacaat agagctgcaa gcgcgacaag cacactggca aaaatgcggt tcttcatttc
      181 gttctccctg aaaagtggca aggcatcgca tccagatggc cactgacatg cggactggaa
      241 atgcgtcttg cgtagcagcg gatcattggc atgaggcaca gcttatcggc gattgcttga
      301 cttgggcttc cgggtcggct tcggctcggg taggcgcaga ccgctgcaaa gcaagctgtt
      361 tttgctcagc ggtgtattgg ttgagcgctg cccgaatccc ggtgccgacg ttcatcatcg
      421 gcagcgcagc ttcgtagcct tctttggcgt cacgttcggc tatcagcgga cttgcgatgt
      481 tgaatgcttc aatgaacgag tcggtcttgc gtaacccgtc ttcaaagtag gctttgccga
      541 aaaacgtgaa atcggcttca ttgctgcagc cgtgcgatgt cttgtcgggc gccgaagcgc
      601 tgatgacgag cgtgttttcg ttcttgagcg gctcgacgaa accgccggaa tagcacgagg
      661 aaacgacgac gacgcgacgt ttgatacccg agtcgtcgag aattctgcgc agtactcccg
      721 gatcgagaac atcaaaacgc attgagccaa aactcaggga aagccggtgt tcccgagaac
      781 cgtgcgaagt cagatacaga aacaggatgt ccttgtcggt gtccattttc ttgccaacct
      841 gattcaacgc aaggcgcagg ctggtgacgc tggcagccgg tgactccagt acggttttcg
      901 gattgtttat cagtgttatt gagcgtcccg tcgtcccgaa gcgcgccttg aagaaattgc
      961 tgacgtagtg gacttccttc atgaacacgt cctgttccga atagccggca acgccgacaa
     1021 aatagagatt gatccggtcg ggctggcttc ggtcgagcga tgccagcgcg tgctcaagga
     1081 ttcgcggctg gctgtaaaac gcgcgttctt ccagcagcgc gttttgctgg gcgcgctcgc
     1141 gggccgctga ttccgcgtcg aagggcacag cccagattgt tcggtatcca ccagccaggg
     1201 cgagtggaaa ccagatcaag acaactcccg acaatacgga gagggccacc ttcttcccgg
     1261 agagatcaaa cagccgggtg gctgcgacgg ccgtcgccag gccaagccac gcgggggcca
     1321 gatgtgcata aagcaggtag taggaatctt gaagatggaa attccgtgcg atcagcgggc
     1381 tgccgagcag ccaaagcaaa agatctccaa gaacgctgat cggaatggcg attgcgctga
     1441 tggccacagc gagcatcagc gtggcttcgg gcctctttgc caggagcgac agcgcccagg
     1501 cggcaatgag gatcatcggc accttgaaca gcaggcccgg caagccgtaa ggcgatagct
     1561 caccttgcgg gcccacactg tagaaatcca tcgcgaactg cagggctatg gtgaccgcga
     1621 tcaagccgac gagccgggag aaagacgcct cgccatcttc aatcctgagt cgcagaaagg
     1681 cggcaccgcg caagccctgt gccaggattc ggactggaaa aagcgatcca aggaaatcgg
     1741 tcattttgat tctcaggcag gatttcacga atgccgccaa tcgaccgcat tcgcaggcac
     1801 acgctctttt ccgcgaagtc cgtcgaatcg actcgtcgtt tcgaagtatt ttaatggaat
     1861 atttcgttcg ccgttgtcga tttcagaagg attggacgac ggatcttgcc tttggcctgc
     1921 cctgtttcga tcagccgacg cgtcttcgca gattggccgc gttgccacga cgatcagttg
     1981 cgcggcatgt tctcggcgcg ccagtcggca agagcgctga ttgtgctgtc caggccggat
     2041 agaagccgga tccctgcaat cggcgccagc ccgcaaacgc cgcgaccgcc gacccatagt
     2101 tcgattgtca tcggcagaat ctgacgcaac tgctgcagca ggccgggaat ctggcgcgac
     2161 cggaaggcga tcgagaacga cagggcgacg atgttggcct ggtgggcgac ggcggcgtgg
     2221 ccgatctcga gcaggggcat ttgcgtaccg agcggaatgc attcggcccc ttcgagtacg
     2281 aacaggcctt cggccatcaa caggccgagc acgtgctgtt cgtcaggcac actggtcagc
     2341 aagatgcgcg ggctgcgaac gccaccgggc agggcggcga tggcctggcg caacaggcgc
     2401 ttggtcagtt cggtaaacag gtgttcttcg aaaacctcga agctgccgtc ttcccaggct
     2461 tcgccaacgc gccgggtaag tggcgccacg gtgtcctgga caaaactctg aaaaccttgg
     2521 cgtgccagtc gctgctgcat cgcctgctgg taacccgcgg catcatgctg cttgatcaga
     2581 atcaacaact caccgagatc ttcgccgtcg atttcggcgc ccatctgacg gctggccggt
     2641 cgcctcggcg ccagttgtgc gagttcctcg ggcgaggcag ccatcagctt tccaggccga
     2701 tgcccttgat ccatcaggcg ttttatcagg cgcagtcgtt cgacctgggt ctccgggtag
     2761 agccgttccc cgttgccgtc acgctcgggc tccggaaagc cgtagcggcg ctcccacatc
     2821 cgcaggacgt ccttggagag cccggtgtca cgctcgactg ccgcaatgtt gaaagcaagg
     2881 gttttcatat ttgtcctgta caaaaaggcg taaaagggtt gacaagccgg tctttgatgt
     2941 ctatcttacg aacaaacatc gcttttgtct aggacaaata tacaaggagt tggacatgaa
     3001 tgcccccgaa cacttttttc tgcccgacgt gcaggcccta accgattccc gcagccttgc
     3061 catccagcag gtcggtgtca gaggactgcg ctacccgctt caggtcgaaa ccgctggggg
     3121 cgagacgctg gccacggtcg ccacgctggg gatgacggtc ggcctgccgc ccgaggtcaa
     3181 gggcacgcat atgtcgcgct tcgtcgaact gctcgagggg tgcagcggtg ccgtgacgca
     3241 gggcagcctc tttcacctgc ttgatgacat gctgtgccga ctcgacgccc aaagcggccg
     3301 catcgagctg gctttcccct atttcatcag gaagacggca ccggtttcgg gcgtcaccag
     3361 ccttctcgac tacgacgcga cgatgatcat tgagcaacgt gaaggcgagc gtgccagctt
     3421 gacgctacgt gtcgtcgcgc cggtaaccag cctttgccca tgttcgaaag gcatttccga
     3481 ctacggcgcc cacaaccagc gttcgcacat cacgcttgaa gcaaagctgc gccggccgat
     3541 ggcgatcgag gagctggttc gcctggccga ggaagaggcg tcgtgcgaag tcttcggcct
     3601 tctcaagcgg ccggacgaga aatgggttac cgagcgcgcc tacgacaacc cgaaattcgt
     3661 cgaggacctg gtgcgcgaca tcgcgttgcg tcttatggag gagccgcgca tcgccgaatg
     3721 gaaggtcgca tccgagaatt tcgagtcgat cc
//
LOCUS       DAORPM010000243         3745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_7879_length_3745_cov_10.773442, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000243 DAORPM010000000
VERSION     DAORPM010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7879_length_3745_cov_10.773442"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..985)
                     /locus_tag="PLD53_10400"
     CDS             complement(257..985)
                     /locus_tag="PLD53_10400"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HRC61369.1"
                     /translation="MMARVDTTETLAVGAVLCDLDGTLLDTVPDLYAAACAMLRDLGR
                     PELPLESIRSYVGRGIPNLVKRVLAGSLEAADDASAPPKEALDSFRRHYARENGRNSK
                     PYPGVIDGLDNLKAQGLPLAVITNKAGAFTLPLLELTGLAAYFDVIVSGDLLPKPKPD
                     PMALIWACGRIGVSPADTLMIGDSVHDFRAGRAAGCHVFLVPYGYNEGLDVRDLDCDA
                     IVSTVAEAAQRIRNLEKSQLTTCA"
     gene            complement(982..1683)
                     /gene="rpe"
                     /locus_tag="PLD53_10405"
     CDS             complement(982..1683)
                     /gene="rpe"
                     /locus_tag="PLD53_10405"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRC61370.1"
                     /translation="MYLIAPSILSANFTRLGEEITDVIAAGADWIHFDVMDNHYVPNL
                     TIGPLVCEAIRPLTQAVIDVHLMVKPVDRIIPDFAKAGANVITFHPEASEHIDRSLSL
                     IRESGCQAGLVFNPATPLDYMDYVMDKLDIVLLMSVNPGFGGQKFIPATLDKLRQARA
                     RIDAYERESGRKIRLEVDGGVKVDNIAEVARAGADTFVAGSAVYGAGKDSDPQRYNTV
                     IAAMRAELDSIRAKA"
     gene            complement(1779..3386)
                     /locus_tag="PLD53_10410"
     CDS             complement(1779..3386)
                     /locus_tag="PLD53_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRC61371.1"
                     /translation="MEPLNDLFQRFEQLNVIGASLSSERNIDRLLENILLAAKAITHA
                     DGGTLYRVREDRKALRFEIVRNDSLSIAYGGTAGKPDPAKFQELPLYRENGEENNSLV
                     AAHSAIHARTVNIADAYTADGFDFSGTRNFDQQTGYRSQSFLTVPMKNHENEVIGVLQ
                     LINAIDPASGAVGVFSDADQRLAESLASQAAIALTNRLLISQLEELFEAFITLINLAI
                     DEKSPYTGGHCQRVPSLTMMLAEAVDATTEGPMSSFMMTDKDRYELKIAGLLHDCGKV
                     TTPVHVVDKATKLQTIFDRIQMVDTRFQVIRRDAELAMLRAVLDGTDRVLAEQTWHEQ
                     LRAIDEDREFLRRANVGGESMKPEDQERVRHISQTYRWVSDEGTEVDFLTDNEVENLT
                     IRAGTLTQEERQTINYHIVATIKMLESLPWPKHLKRVPEYAGGHHERMDGKGYPRGLK
                     RDEMSVQARIMGIADIFEALTARDRPYKPGMKLSQALRILGNFKKNGHIDPDLFDVFI
                     KQKVYLKYAEQYLEASQIDEPEASPDT"
     gene            complement(3398..>3745)
                     /locus_tag="PLD53_10415"
     CDS             complement(3398..>3745)
                     /locus_tag="PLD53_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system protein E"
                     /protein_id="HRC61372.1"
                     /translation="EELSNTDHWKKDPKVAANEVHKEWLKHYAKDSKFTLYRAKGCDV
                     CAESGYKGRVGLHELLIGTDLLKRQIQEHAHVAELLATALNEGMRTLKMDGIEKIMSG
                     ITDIKQVRSVCIK"
BASE COUNT          759 a         1070 c         1078 g          838 t
ORIGIN      
        1 ccagcttcag gtagatcgag agcggggtgt cgagatcggc aaaggtctca agggtgacgg
       61 gaatacggtt gtagccttcg gcggccagcc gattgaaata cgcttcagtc atgaaatgct
      121 ccacaaacga ttactgcatc aagaggcacg gcacaacgtt tgtaccgtgg caaggcccag
      181 gtttactggc agaaacgcca gggccacgcc tccgcccaaa gagcaggctg acgatgcaga
      241 ttgtagtgaa gagcattcat gcgcaagttg tgagttgtga tttttccagg tttctgatgc
      301 gttgcgcagc ttcggcaaca gtggaaacta tagcatcgca atccagatcg cgcacgtcaa
      361 gcccctcgtt gtagccgtag ggaacgagga agacatggca gccggcggca cggccggcac
      421 gaaaatcatg gactgaatcg ccaatcatca aggtatcggc cggggaaacg ccaatacggc
      481 cgcaggccca gatcagcgcc atcgggtcgg gttttggctt cggcagaagg tcgccgctga
      541 cgatcacgtc aaaatatgca gccagtccgg tcaattccag aaggggcagc gtaaaggcgc
      601 cggccttgtt ggtgatcacc gccagcggaa gtccttgcgc tttgagattg tccagcccgt
      661 cgatcacgcc gggatacggt ttggagtttc gtccgttttc tcgcgcatag tgccgtcgga
      721 aactgtccaa cgcctccttt ggcggtgccg aggcatcgtc agccgcctcc agggacccgg
      781 caaggacgcg cttgaccagg ttcgggatgc cccgccctac gtagctgcgt atcgactcca
      841 gaggcaattc cggccggccg agatcgcgca acatggcaca ggcggccgcg taaagatccg
      901 gcacggtgtc gagtagcgtg ccatccagat cgcacagaac ggcgcctacg gccagcgttt
      961 ccgttgtatc gacacgtgcc atcatgcttt ggcccttatg ctgtccagtt cggcacgcat
     1021 tgcggcaatg accgtgttgt agcgctgtgg atcgctgtcc ttgccggcgc cgtagactgc
     1081 cgagccggca acgaaggtat cggcgccggc acgggcgact tcggcaatgt tgtcgacctt
     1141 gaccccaccg tcaacttcca ggcgaatctt gcgaccactc tcgcgttcgt aggcgtcgat
     1201 ccttgcgcgg gcctgcctga gcttgtcgag tgtcgccgga atgaacttct gtccgccaaa
     1261 gccgggattc acgctcatca gcagaacgat atcgagtttg tccatgacgt aatccatgta
     1321 gtcgagcggt gtcgccggat tgaaaacaag acccgcctgg caaccgcttt cacgaatcaa
     1381 cgaaagactg cggtcgatat gctccgacgc ctcgggatga aaggtaatga cgttcgcgcc
     1441 ggctttggcg aaatccggaa tgatgcgatc gaccggcttg accatcagat gcacgtcgat
     1501 gaccgcctgg gtcagtggac gtatggcttc gcacacgagc ggcccgatgg tcaggttggg
     1561 cacgtaatga ttgtccatga catcgaagtg aatccagtcg gcgccagcgg caattacatc
     1621 ggtgatttcc tctccaagcc tggtgaaatt ggcggaaagg atactggggg cgatgaggta
     1681 catggcaatg acctgctggg aagaagaaaa cgagcatttt accgcggatg gcgcacgagc
     1741 cggcggctgt tgcgcagcct ttggtcctta tcccgcgatc acgtatccgg cgaagcctct
     1801 ggttcgtcga tctgcgatgc ttcgaggtat tgctcggcat acttcagata aaccttctgc
     1861 ttgatgaaaa cgtcaaacag gtcggggtca atgtggccgt tcttcttgaa gttgccgaga
     1921 atgcgcagcg cctgcgagag cttcatgccg ggcttgtagg gtcggtcgcg tgcggtcagc
     1981 gcttcgaaga tgtcggcgat tcccatgatc cgcgcctgaa ccgacatctc gtcgcgcttg
     2041 agccccctcg gatagccttt gccatccatg cgctcgtggt gaccgccggc gtactcgggc
     2101 acccgcttga ggtgcttcgg ccagggtagt gattcaagca ttttgatcgt tgcgacgata
     2161 tggtaattga ttgtttgccg ttcttcctgg gtcagcgtcc cggcacggat ggtcaggttc
     2221 tcaacttcgt tatcggtcag aaaatcgact tcagtgcctt cgtcgctgac ccagcgatag
     2281 gtctgcgaga tatgccgtac gcgttcctga tcctcgggct tcattgattc accgccgacg
     2341 ttcgcgcgac gcagaaattc gcggtcttcg tcgatggccc gcaactgttc atgccaggtc
     2401 tgctcggcga gcacacggtc cgtgccgtcc aacacggcgc gcagcatggc cagctcggcg
     2461 tctcgtcgga tcacctggaa gcgtgtatcc accatctgaa tccggtcaaa gatcgtttgc
     2521 agcttggtcg ccttgtcgac cacatgcacc ggggtcgtca ccttgccgca atcgtgtaac
     2581 aaaccggcga tcttcagctc gtacctatcc ttgtcagtca tcatgaaact ggacattggc
     2641 ccctcagtgg tcgcatctac cgcctcggcc aacatcatcg tcagggacgg aacacgctgg
     2701 caatgtccgc cggtgtaggg tgatttctcg tcaattgcca agttgatgag tgtgataaag
     2761 gcctcgaaca attcttccag ctggctgatc agcaggcggt tggtcagggc aatggcggct
     2821 tgcgaagcca gcgactcggc gagccgctgg tcggcgtcgg aaaatactcc tacagcgccg
     2881 cttgccggat cgatggcatt gatcagttgc agaacaccga tgacctcatt ctcgtgattc
     2941 ttcatcggca ccgtgagaaa tgactgcgag cgatagcccg tctgttgatc gaaatttctg
     3001 gtgccggaaa aatcgaagcc gtcggcggtg taggcatcgg caatattcac cgtcctggca
     3061 tgaatcgccg aatgggcggc caccagggag ttgttttcct cgccattctc ccgatagagc
     3121 ggcaattcct gaaacttggc cggatccggt ttgccggctg tcccaccgta ggcaatgctc
     3181 agtgagtcgt tacgcacgat ttcaaaacgc aaggctttcc ggtcttcccg aacgcggtag
     3241 agcgtcccgc catcagcatg cgtaatcgcc tttgccgcga gcaggatgtt ttccagaagt
     3301 cggtcaatat tccgctcgct ggatagcgaa gcaccgatga cgttgagttg ctcgaagcgt
     3361 tgaaacaagt cgtttagggg ttccacggca ttcactccta cttgatgcaa accgaacgaa
     3421 cctgcttgat gtcggtaatc cccgacatga tcttttcgat gccgtccatc ttcagcgtac
     3481 gcatcccttc gttgagcgcc gtggccaata gctcggcgac atgagcatgc tcctgtatct
     3541 gccgtttcaa cagatcggta ccgatcagca actcgtgcaa accaacccgc cccttgtagc
     3601 cgctctcggc gcatacgtcg caacctttgg cgcgatagag cgtaaatttg ctgtccttgg
     3661 cgtaatgctt caaccattcc ttgtgtactt cgttggcggc caccttggga tctttcttcc
     3721 agtgatcggt attgctcagc tcctc
//
LOCUS       DAORPM010000244         3672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8154_length_3672_cov_9.993365, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000244 DAORPM010000000
VERSION     DAORPM010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8154_length_3672_cov_9.993365"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLD53_10420"
     CDS             <1..443
                     /locus_tag="PLD53_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRC61373.1"
                     /translation="AWVLLKGMETLKIRLEAQSSKALELARWLESHPKVTRTYYPGLE
                     SHPQFELARRQQKSGGAIVAFEVKGARSEAWKVIDNCRLLSITANLGDTKTTLTHPAT
                     TTHGRITPEQRAAAGITESLLRVAVGLEAVVDLQNDLDVGLSQI"
     gene            complement(470..736)
                     /locus_tag="PLD53_10425"
     CDS             complement(470..736)
                     /locus_tag="PLD53_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HRC61374.1"
                     /translation="MQLTQRHKAYWRRTFWMTAILLVIWFGVTFVVSYYARELSAISL
                     FGFPFGFYMGAQGTLVIYVLIIWFYVRYMNALDREFGVSEGEDG"
     gene            complement(845..2881)
                     /locus_tag="PLD53_10430"
     CDS             complement(845..2881)
                     /locus_tag="PLD53_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRC61375.1"
                     /translation="MRNSTVQLRRYYGLYTGSFLSFLLALGILEYFGFPSRWIGYIFL
                     LLTIVLYATIGVMARTSNVAEYYVAGRRIPAMFNGMATGADWMSAASFIGLAGTLFLT
                     GYQGLAYVMGWTGGFVLVALLLAPYLRRFGQYTIPDFLSARYGGSVARLVGVSATVLA
                     SFVYVVAQIYGVGLITSRLVGLQFEIGVFVGLAGILVCSLLGGMRAVTWTQVVQYLVL
                     IVAYATPVAILSYTMTGVPVPQIMYGQVLPKIDLLEDKITHDPAEIEARRLYLERADT
                     LSALITGLPESLAEEKRDLSVRIDQLKNREARIPDLIALERQRRELPKTPDAAHDYWD
                     SQMRHDARQGSALIRHLEPYPGKDEGAKNSARLNFVTLVFCLMLGTASLPHVLMRYYT
                     TPSVREARSSVFWSLLFILAFYVTAPAYAVFAKYEIYSNLIGSSIAELPSWVGAWSRI
                     GLVSIEDINHDGILQLAELSLNPDVIVLAMPEIAGLPYVISGLVAAGALAAALSTADG
                     LLLTIAGAFSHDVYYKVLRPNATTQWRLIVSKTLLLIVAVLAAIVASQKPASILSMVA
                     WAFSLAGAAFFPALVLGIFWKRANKAGAVSGMVLGLLVTLYYLVRVQFNSIPWLGIRD
                     IGMEPWLGIESTSAGIWGVVVGFVTIFVVSLFGEPPSPETQNFVESVRSPDFSR"
     gene            complement(2900..>3672)
                     /locus_tag="PLD53_10435"
     CDS             complement(2900..>3672)
                     /locus_tag="PLD53_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRC61376.1"
                     /translation="SGAQAPKASQTRLSALTMSWLHRFFGSNKTERQASLSPEHSQAI
                     EAWQQLPAEDLNRAHNRCRYVVVDVESSGLDMRKDRLISIGAVALVHGKIDFKDAFQV
                     VLRQDKASSHENILIHGIGGMAQREGMDPAEALIAFLRYIGKSPLVAYHAFFDQALIE
                     KAMHEYLGMELGQTWIDLAWVLPSFFQFQTDARMELDDWLETFGIDSFLRHNAVSDAY
                     ATARLLQLAIAAGNQKGSDSPASFLEIEKARRWMHERR"
BASE COUNT          917 a         1071 c          978 g          706 t
ORIGIN      
        1 acgcctgggt cctgctgaaa gggatggaaa ctctcaagat caggcttgaa gcccagtcga
       61 gcaaggccct ggaactggca cgctggctcg aatcacaccc caaggtcacc cgaacctact
      121 accccggtct cgaatcgcac ccgcagttcg aactcgccag gcgccagcag aaaagcggtg
      181 gcgccatcgt cgccttcgaa gtcaaggggg ctcgaagcga agcgtggaag gtaattgaca
      241 actgtcgttt gctttcaatc accgcaaatc ttggcgatac gaagaccacg ctcacgcacc
      301 cggcgacaac aactcatggc cgcatcacac ccgaacaacg tgcggcagcc gggatcaccg
      361 agtctttatt acgcgtggca gtcggtctgg aagccgtcgt cgatttgcag aatgacctgg
      421 acgtcggtct ttcgcagata tagcagttca tcggtcggat cgcccacgac tatccgtcct
      481 cgccttccga cacaccgaac tcccgatcga gagcgttcat gtaccggaca tagaaccaga
      541 tgatcaacac atagatgacc agcgttccct gagcacccat gtaaaagccg aaggggaatc
      601 cgaacagcga aatcgcgctg agttcacggg cgtaatagct gacaacgaag gtgactccaa
      661 accatataac cagaaggatt gctgtcatcc agaaggttcg cctccagtag gctttgtgcc
      721 tttgcgtaag ttgcactgaa cgtcatcctt ttgcaagata gctgaagaag tgtcgcaatc
      781 atcatgactg cctgcgcaag gtacaggcga cacccatact acacttccat gatacgttcc
      841 gttcctagcg agagaaatca ggggaacgga cactctcgac gaagttctgc gtctcgggac
      901 tgggtggttc gccaaacagg ctgacaacga aaatggtcac aaaccccacg acgactcccc
      961 atataccggc ggacgttgac tctatgccca gccacggctc catgccaata tcacgaatac
     1021 cgagccaggg aatcgagttg aactgcacgc gaaccaggta gtacagggtc accagcagtc
     1081 cgagcaccat gccgctgacg gctcctgcct tgttggcgcg cttccagaat atgccgagca
     1141 ccaatgccgg gaaaaaagcc gcacccgcca gcgaaaatgc ccaagccacc atggaaagaa
     1201 tgctggcagg tttttgtgag gcgacaatcg ccgccagcac cgcaacgatg agtagcagcg
     1261 ttttggaaac gatcaggcgc cactgcgttg tcgcattggg ccgtaagact ttgtagtaca
     1321 cgtcatgcga aaaggcccca gcaatcgtca gcaacaaccc gtcagcggtc gacaaggccg
     1381 cagccaaagc accagccgcc accagcccgg agattacgta gggcaggccg gcaatttcgg
     1441 gcatcgccag aacgattaca tcgggattca aagaaagctc ggcgagctga aggatgccgt
     1501 catgattgat atcctcgatc gataccagtc cgattctgct ccacgcgcca acccagcttg
     1561 gcaattcggc gatacttgaa cctatcaggt tcgagtagat ctcgtacttg gcaaagaccg
     1621 cataggcggg ggcggtcacg tagaaggcga ggataaacaa tagagaccag aaaacagaac
     1681 tgcgtgcttc gcgcacactg ggcgttgtgt agtaacgcat caggacgtgc ggcaaggaag
     1741 ccgtaccgag catcagacaa aacacgaggg tcacgaaatt gagtcgcgcc gaattctttg
     1801 cgccctcgtc ctttcccgga tagggttcca gatggcggat aagcgcactg ccctgccggg
     1861 cgtcatgccg catctgtgaa tcccagtaat catgtgcggc atcgggagtt ttcggcaatt
     1921 cccggcgctg tcgctccaga gcaatgagat cgggaattct tgcttcgcgg tttttcaact
     1981 ggtcgatccg taccgaaaga tcacgcttct cttctgcgag tgattcaggc aggccggtta
     2041 tcagcgcaga tagcgtatcg gcgcgctcca gatacaggcg tcgcgcttca atctcggcag
     2101 gatcgtgtgt aatcttgtcc tcaagcaggt cgatcttcgg caatacctga ccgtacatga
     2161 tctgaggtac cggtacgcca gtcatcgtgt acgaaaggat cgctaccggg gtggcgtaag
     2221 cgacaatcaa tacgagatac tggacgacct gcgtccacgt cactgcgcgc atgccgccga
     2281 gcagggaaca caccagaatc ccggccagac cgacaaatac cccgatctcg aactgcaagc
     2341 cgaccaggcg actggtaata agacccacgc cataaatctg ggcaactaca taaacgaatg
     2401 atgccagcac agtggcagag acaccgacca ggcgcgccac gctccccccg tagcgggcgc
     2461 tgagaaaatc cggaatggtg tactggccaa accgccgcaa gtaaggtgcg agcagtagcg
     2521 cgaccagcac aaaacctcct gtccagccca ttacataagc cagcccctga tagccggtga
     2581 gaaagagcgt gcccgccagg ccaatgaatg acgcagcact catccagtcc gcgccggtgg
     2641 ccatgccatt gaacatcgcc ggaattcttc gaccggcgac atagtattcc gccacgttgg
     2701 atgtccgcgc cattacgccg atggtggcgt acaaaacgat ggtcaggaga agaaagatgt
     2761 acccgatcca gcgcgaagga aaaccgaaat actcaaggat gcccagcgcc agaaggaacg
     2821 aaagaaaact tcccgtatag aggccgtagt acctgcgcag ttgtacggtg gagttcctca
     2881 tctggcagtc tgtactcctc tagcggcgct catgcatcca gcgccgtgcc ttctcgattt
     2941 caagaaaact ggccggcgaa tcggacccct tctgatttcc ggcggctatc gccaactgca
     3001 atagcctcgc tgtggcataa gcatccgaaa ccgcgttgtg ccgcaagaag ctatcgatgc
     3061 caaaagtctc cagccagtcg tcaagttcca ttcgggcatc cgtctggaac tggaagaagc
     3121 tcggaagcac ccatgcgaga tcaatccatg tctgcccgag ttccattcca aggtattcat
     3181 gcatcgcttt ttcgatcaag gcctgatcaa agaaagcgtg ataggcaacc aagggagatt
     3241 tgccgatata ccttaggaat gcgatcagcg cttcagccgg atccatgcct tcacgttggg
     3301 ccatgccgcc gataccatga atgagaatgt tctcgtgact gctcgcctta tcctgccgca
     3361 gtacaacttg aaaagcatcc ttgaaatcga tcttcccatg gaccagcgcg accgcgccga
     3421 tcgagatcag gcgatccttt cgcatgtcaa gtccgctgct ttcaacgtcg acgaccacat
     3481 atcggcagcg gttgtgggca cggttgaggt cttcggcggg caactgctgc catgcctcga
     3541 tcgcctgact atgttctggc gaaagactgg cttgtcgctc cgtcttgttg ctgccgaaaa
     3601 acctgtgtaa ccacgacatg gtcaaagctg ataatcgagt ttgagacgct tttggagctt
     3661 gcgcgcctga cg
//
LOCUS       DAORPM010000245         3622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8356_length_3622_cov_9.552004, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000245 DAORPM010000000
VERSION     DAORPM010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8356_length_3622_cov_9.552004"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1319)
                     /locus_tag="PLD53_10440"
     CDS             complement(66..1319)
                     /locus_tag="PLD53_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRC61377.1"
                     /translation="MSILRFLSIPLLIASSTLGLVFWAFPAGLAALRMLGIVLGWAGC
                     GLLLAGLLLMLRETWLARGLDGLERMYWWHHRVGMAAYVLLLAHPLALAAAAWQESRL
                     LAWQTLSPFSQGWPVWMGWLSLVLLMLGLAATFETRIPYRIWRWLHVGLGAGVLLGLV
                     HLVLLGIDEPVLPILTVAALLLGWRLLRADFGIAARPYVVQSTQRVAEGMIEISLSPL
                     GNPVAVNAGQYVLVAFFAGPRFRGCGEFHPFTVSSTGGDGQIRIAVKALGDCTQHLQS
                     IESGVMARIHGGFGRFLADTPTTPQLWIAGGIGITPFLAVLRGGLVAQPTKLLYLYRV
                     KTDAAFLQELQDLADEDPKLSLQAMVTGNVPPDLESLLPDAQGLSGHECYVCGPPGMI
                     AALKQALQHRGITPRHIHFENFGFR"
     gene            complement(1350..1643)
                     /locus_tag="PLD53_10445"
     CDS             complement(1350..1643)
                     /locus_tag="PLD53_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61378.1"
                     /translation="MMKMELLIAALLLTGPVLAWGPRHASEENTRGWQFMTPEERIEH
                     QSRIRRFTSYEECHAYQASHHRMMEERARKRGMELPIGRRDICEHLKPEAVTR"
     gene            complement(1694..2014)
                     /locus_tag="PLD53_10450"
     CDS             complement(1694..2014)
                     /locus_tag="PLD53_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61379.1"
                     /translation="MTMLEGERTDYIGKTGRRRRRAANGRLYAYGIEPESGCGRTPLI
                     EVPNVWPVLKIKPLDLGLLLITHKQPMCASGVVLTTPLLYFLRAHWSEDGYLQPERRN
                     TFDL"
     gene            complement(2088..2792)
                     /locus_tag="PLD53_10455"
     CDS             complement(2088..2792)
                     /locus_tag="PLD53_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC61380.1"
                     /translation="MHPAIEISGLTKRYGRGDAAVDALKGVDMIVQPGEVVGLIGPSG
                     SGKSTLLKCLGAVIEPTSGRMALAGETIYDNGWKVADLRALRRDRIGFVFQTPYLIPF
                     LDVIDNVALLPMLAGVSNGKARDRARAMLVALDVGHRAQAQVPQLSGGEQQRVAIARA
                     LVNQPPIILADEPTAPLDSERALTVVRILNEMAQRFGTAIIVVTHDEKIIPTFQRIYH
                     IRDGRTYEQAGEARAA"
     gene            complement(2793..>3622)
                     /locus_tag="PLD53_10460"
     CDS             complement(2793..>3622)
                     /locus_tag="PLD53_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRC61381.1"
                     /translation="YLVAGRPITRSHYEAIADVKTGFQLDERIRIRRHEYTVVGLTRR
                     MVSSGGDPMVFVPLQDAQEVQFLKDNDALVNERARTAANPAFNRPGVPGLLEAVQASQ
                     ASSRTVNVVMLRLASNADEMVVAREIRRWKHLEAYTRTDMDEILVAKLIATSAKQIFM
                     FLVILAVVSAAIVAFIIYTMTLNKVREIAVLKLIGARNRTIAGMILQQALGLGAIAFV
                     VGKTVATLWAPIFPKYVLLLPGDAVLGAVLVLVICAFASIGAIRAALRVDPATAIGG"
BASE COUNT          736 a         1153 c         1082 g          651 t
ORIGIN      
        1 ccctatccag aacacggcca ccaacagcca gaaggcaacg gtggcggata aatagagtcg
       61 gcgcattatc taaacccgaa attctcaaag tggatatggc ggggtgtgat accgcggtgc
      121 tgaagggctt gcttcagtgc ggctatcata ccgggcggcc cgcagacata acactcgtgg
      181 ccggatagtc cttgtgcatc cggaaggagc gactccagat ccggggggac attgccggta
      241 accatggcct gcaaggaaag ctttgggtcc tcgtcggcaa ggtcttgcag ttcctgcaga
      301 aacgcggcgt cggtcttcac ccgataaaga tagagaagtt tggttggctg tgcgacaagc
      361 ccgcccctca ggaccgccaa gaagggcgtt atgccaatcc caccggcgat ccatagttgc
      421 ggggttgtgg gcgtgtccgc caggaagcgg ccaaacccac catggatccg ggccattacg
      481 ccggattcga tggactgcaa gtgctgcgtg caatcgccca aggccttgac cgcaatgcgg
      541 atctgaccgt cgccccccgt cgagctgacg gtaaacggat gaaattctcc acagcctcgg
      601 aatctcggcc ctgcgaagaa ggccaccaga acatactgcc ccgcgttaac ggcaacgggg
      661 ttgcccagcg gggacaggga aatctcgatc atgccctcgg cgactcgttg agttgattgc
      721 acgacgtagg ggcgtgcggc gattccgaaa tccgcccgaa gaagacgcca ccccagcaac
      781 aaggcggcca ccgtaagaat cggcagcacg ggctcatcga tccccagcaa aacgaggtga
      841 accagcccga ggagcacacc ggcgcccagc ccgacatgga gccaacgcca gatccgataa
      901 ggaatccgcg tctcgaaggt ggccgctaag cccagcatca ggagcaccag tgacaaccat
      961 cccatccaca ccggccagcc ctggctgaac ggagaaagcg tctgccacgc cagcaggcgc
     1021 gactcctgcc aggcagcggc agcgagcgcc agtggatgag ccagcagcag gacatacgcc
     1081 gccatgccga ctcgatgatg ccaccagtac atcctttcga gtccgtcaag cccgcgggcc
     1141 agccaggttt ccctcagcat cagtagaagt ccggcgagca agaggccaca cccggcccac
     1201 cccaggacga ttcccagcat tcgcaacgcc gccagccccg ctggaaacgc ccagaacaca
     1261 aggccaagcg tggaactcgc aatcagcagc ggaatggata ggaagcgcaa tatagacata
     1321 agtgtctgcc gagtcgactg accggaatgc tagcgcgtga ccgcttcggg cttcaggtgt
     1381 tcgcagatgt cccgcctacc tatgggtaat tccatgccgc gctttctggc gcgctcttcc
     1441 atcattcgat gatggcttgc ttggtatgca tggcactcct catagctcgt aaaacgacga
     1501 attctggatt ggtgttcgat ccgctcttcc ggcgtcatga attgccagcc ccgggtattc
     1561 tcttcactgg cgtgccgcgg tccccatgca aggacaggcc cggtaagcag cagcgctgcg
     1621 atcaacaatt ccattttcat catgctatcc ctaccgcttt ggtcctcgtg agccgcccat
     1681 cgccaacgtt gtgtcaaaga tcaaaagtat tcctgcgctc cggttgaaga tacccgtctt
     1741 ccgaccaatg cgcccgcaga aaatacagaa gtggtgtggt caggacgacg ccggaagcgc
     1801 acattggctg tttatgggtg atgaggagca gaccgagatc tagcggtttg atcttgagca
     1861 ccggccacac gtttggcact tcgatgagcg gcgtgcggcc gcagccgctt tcaggctcaa
     1921 tcccatatgc gtacaatcgg ccattcgcag cccgtcgccg ccgcctccca gttttcccta
     1981 tatagtcagt ccgttcgcct tccagcatgg tcaagagcag tacgttcttg tacgggagat
     2041 tggaacgaaa tgtcatcttt ggtttaacgg tcgatcgcgc gccttgatca ggcggcgcgt
     2101 gcctcgcctg cttgctcgta ggtgcgtccg tcgcggatat gataaatccg ctggaatgtc
     2161 gggatgatct tctcgtcgtg ggtgacgacg atgatcgccg ttccgaaccg ttgcgccatc
     2221 tcgttcagaa tgcgcaccac ggtcagcgcc cgctcgctgt caagcggtgc cgtcggctcg
     2281 tcggcgagga tgatcggcgg ctggttcact agcgcgcggg cgatcgcgac gcgttgctgc
     2341 tcgccacccg aaagctgggg cacctgcgcc tgggcgcgat gaccgacatc cagcgcgaca
     2401 agcatcgccc gcgcccggtc gcgcgccttg ccgttggaca caccggccag catcggcaac
     2461 agcgcgacat tgtcgatcac gtcgaggaac gggatgagat agggcgtttg gaacacgaag
     2521 ccgatccggt cgcggcgcaa cgcgcgcaga tcggcgacct tccagccgtt gtcgtagatc
     2581 gtctccccgg caagcgccat gcggccggaa gtgggctcga tcacggcgcc caggcatttc
     2641 aacagcgtgc tcttgcccga gccactggga ccaatcagtc ccacgacctc gccgggctgg
     2701 acgatcatgt ctacgccttt caacgcgtcg accgctgcat cgccccggcc gtagcgcttg
     2761 gtgaggccgc tgatttctat cgccggatgc atctatcccc cgatggccgt cgccggatcg
     2821 acgcgcaacg cggcgcggat ggcgccgatg ctcgcgaacg cgcagatcac cagcacgagc
     2881 acggcgccca gcaccgcatc cccgggcagc agcaacacgt atttggggaa gatcggcgcc
     2941 cacaaagtgg cgacggtctt tccgacgacg aaggcgatag cgcctaggcc gagcgcctgc
     3001 tgcaggatca tcccggcgat ggtgcggttg cgcgcgccga tcaacttgag caccgcgatc
     3061 tcgcgcacct tgttcagggt catcgtgtag atgatgaaag cgacgatggc ggcgctgacc
     3121 acggcgagga tgaccagaaa catgaagatc tgcttggccg acgtggcgat caacttggcg
     3181 acgaggattt cgtccatgtc ggtccgcgtg tacgcttcca gatgcttcca acgccgaatc
     3241 tcgcgcgcca ccaccatctc gtcggcgttc gacgccagtc gcaacatgac gacattcacg
     3301 gtacggctgc tggcttggct cgcctgcacc gcctcgagca gtcccggaac cccggggcgg
     3361 ttgaacgccg ggttggcggc ggtgcgcgcc cgctcgttca ccagcgcgtc gttgtccttg
     3421 aggaactgca cttcctgcgc atcttgcagc ggcacgaaca ccatggggtc accgcccgac
     3481 gacaccatgc gccgggtcaa gccgaccacc gtgtactcgt gccggcggat acggatgcgt
     3541 tcatcaagtt ggaaccccgt cttgacatcg gcgatcgcct cgtagtgcga acgcgtgatc
     3601 ggacgtccgg cgacgaggta gc
//
LOCUS       DAORPM010000246         3571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8569_length_3571_cov_14.806030, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000246 DAORPM010000000
VERSION     DAORPM010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8569_length_3571_cov_14.806030"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLD53_10465"
     CDS             complement(<1..845)
                     /locus_tag="PLD53_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61382.1"
                     /translation="MNYLAGVTLTNPLAAEQQLMHFIDALLAAPPDTETMLALLEKAR
                     APICFVEGEMAKIYHNKPLVLNEQEEASFQQVLSAWQKLLKAYALCVQLEKPAADNTQ
                     QVSLMASILHRCIYYTGMIILEHYRARRELPRGIWLEMHGFYETAEEWGLAYTPVEDS
                     LENSFQSTHCAAAYATMLLIDVASPYTNSVRDLNLIRRWASMWAPLISIHQLDDDLEI
                     PPYIVELFKDSPLHPSALVEVPDSYARRLDTTRLGLQITHMLSQLRQRISPSQLGLGE
                     ETSGH"
     gene            complement(901..1284)
                     /locus_tag="PLD53_10470"
     CDS             complement(901..1284)
                     /locus_tag="PLD53_10470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRC61383.1"
                     /translation="MLWESPTCRVIRVDDPHYPGFCRVIWNAHVREMTDLDPGQQQYL
                     MSVVFAVEAVLRQLVSPDKINLASFGNMVPHLHWHVIPRWQDDRNFPEPIWGSVQRAG
                     SEKRPAISNRELAAALSLALNSAEK"
     gene            complement(1440..>1523)
                     /locus_tag="PLD53_10475"
     CDS             complement(1440..>1523)
                     /locus_tag="PLD53_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HRC61384.1"
                     /translation="AKHLLGENWMADFVAKANTGGIERVLL"
     assembly_gap    1524..1623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1624..>3571)
                     /locus_tag="PLD53_10480"
     CDS             complement(<1624..>3571)
                     /locus_tag="PLD53_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRC61385.1"
                     /translation="AASEVVRMCNLRAAEGFIAVDAETRKKFWLDRSRTAAISRHTNA
                     FKVNEDVVIPLPRMGDYCDGIERINIELSISNKLALCDSLLEFLKGDLPLHRGDTTLD
                     TKVLIGDRRQAAIDYVETVRLRWAWLLASLDMPLAEAELHFPTYAVQAGELTNRAENP
                     RLFHRLQDYSVRVSWKQELHARLSKIFDGGVYRPTVERIEAIHKEVLRGRVFVALHMH
                     AGDGNVHTNIPVNSDNYDMLQTAHKAVERIMHLARSLDGVISGEHGIGITKLEFLSEE
                     EIAPFRAYKQKIDPEGHFNKGKLMPGGDMGNAYTPSFSLLGTESLIMEQSEIGKISDM
                     VKDCLRCGKCKPVCSTHVPRANLFYSPRDKILATSLLVEAFLYEEQTRRGISLKHFDE
                     FNDVADHCTVCHRCVKPCPVDIDFGDVSVAMRNFLRKQEKKRFSPGTAAAMTYLNLKD
                     PATIKLMRFGMMDFGFKAQRLAHKAAKALGLIQDTRAHPPASLGAPSVKTQVIHFLNR
                     PMPGNLPKRTSRALLDIEDDKVIPVIRNPRKTTEESDAVFYFPGCGSERLFSQVGLAT
                     QAMLYEVGATTVLPPGYLCCGYPQSASGDDDKGQKITTDNRVLFHRVANTLNYLDIKT
                     VIVSCGTCMDQLQKYQFDKIFPGCR"
BASE COUNT          771 a          920 c         1009 g          771 t
ORIGIN      
        1 atgtgaccac ttgtttcttc gccgagcccg agctgagagg gtgaaatccg ttgtctcaac
       61 tggctgagca tgtgggtgat ttgcagacca agccgagtgg tatcgagccg tcgcgcataa
      121 ctgtcgggta cttcgacgag cgcactggga tgtagcggcg agtctttgaa tagctcgacg
      181 atataaggcg gaatctccag gtcatcatcg agttgatgaa tcgaaatcaa cggtgcccac
      241 atggaagccc agcgccggat caggttcagg tctcggacac tgttggtgta agggctggcc
      301 acatcgatta atagcatcgt ggcgtaagcg gccgcacaat gagtcgactg aaaactgttt
      361 tcaagcgagt cttcgacggg cgtataggcc aatccccatt cttcagccgt ttcatagaaa
      421 ccgtgcatct caagccagat acctcgcggc agttctcgcc ttgcccggta gtgctcaaga
      481 ataatcattc ccgtgtaata aatacaacga tgcagaatgg atgccatcag cgagacttgc
      541 tgagtattgt cagctgccgg cttttcaagc tgaacgcaca gggcataagc tttaagcaat
      601 ttttgccagg cgcttagcac ctgttggaag ctcgcttctt cctgctcatt cagaacaagc
      661 ggcttattgt gataaatctt ggccatttcc ccttcaacga agcagatcgg tgcccgagct
      721 ttttcgagca aggccagcat cgtttcggta tcaggcggtg ctgcgagcag cgcatcgata
      781 aaatgcatga gttgctgctc ggcagccaac ggattggtca gcgtcacgcc ggcaagataa
      841 ttcaatccgc tttgtagatc gaaaatgtga agttgaggtg cgctcatgat ggctgtgacc
      901 tcacttttcc gcgctgttca gtgctagtga aagcgctgca gccaattccc gattgctgat
      961 ggcaggacgt ttttctgaac ccgccctctg caccgatccc cagatgggtt ccggaaaatt
     1021 tctatcgtct tgccagcgag gaatcacgtg ccaatgtaaa tgcggcacca tgttgccgaa
     1081 gctggccaga ttgattttgt ctggtgaaac aagctgcctc aaaacagctt caaccgcgaa
     1141 taccactgac atcaggtatt gctgctgtcc tgggtcgaga tcggtcatct cgcggacgtg
     1201 ggcgttccag atgactcggc aaaaacccgg gtagtgtggg tcgtcaaccc ggataacccg
     1261 gcaggtcgga gattcccaaa gaatatctcc gcccgctgtg gcacatagtt cgcagtgatt
     1321 agcccgctga gtcacgttta aatatcccgg caaaaaattt tcaccgggat attagtaccg
     1381 cagacgacaa cttaaacgca aacaagaact tcacggtcaa ccgctgaaaa cagcaaaaat
     1441 cagagtagga cgcgctcgat tccgccggta ttggctttgg ccacaaaatc ggccatccag
     1501 ttttcaccga gcaggtgctt ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnncggcagc cggggaaaat cttgtcgaac tgatatttct gcaactgatc catgcaggtg
     1681 ccgcacgaga cgatcaccgt cttgatatcg aggtagttca acgtattggc cacgcggtgg
     1741 aaaagcacgc gattgtcggt cgtgattttc tggcccttgt cgtcgtcgcc tgatgccgac
     1801 tgcgggtagc cgcagcacag gtagcccggc ggcagcacgg tggtggcgcc gacttcataa
     1861 agcatcgcct gggtcgcaag accgacctgt gaaaacagtc gttcggagcc gcagcccggg
     1921 aaatagaaga cggcatccga ttcctcggtg gtcttgcgcg ggttgcggat gacgggaata
     1981 actttgtcgt cttcgatgtc gagcagggcc cgcgaggtgc gtttcggcaa attaccgggc
     2041 atcgggcggt tgaggaagtg aataacctgt gttttgactg atggcgcgcc gagcgaggcg
     2101 ggcgggtgag cgcgggtgtc ctgaatcagg ccaagtgcct tcgctgcctt gtgggccagg
     2161 cgttgggcct tgaagccgaa atccatcatg ccgaaacgca tcagcttgat cgttgccggg
     2221 tctttgaggt tgagataggt catggcggct gccgtgcctg ggctaaaccg ttttttctcc
     2281 tgcttgcgca ggaagttgcg catggccacg gaaacatcgc caaaatcgat gtcgaccggg
     2341 caaggtttga cacagcggtg acagaccgtg caatggtcgg caacgtcgtt gaattcgtca
     2401 aaatgcttca acgaaatgcc gcgccgggtc tgctcttcat aaaggaaggc ttcgaccagc
     2461 agcgaagtcg cgagaatctt gtcgcgcggc gagtagaaca ggttggcgcg cggcacgtgc
     2521 gttgagcaaa ccggcttgca tttaccgcag cgcaggcagt ccttgaccat gtcggaaatc
     2581 ttgccgattt ccgactgctc cataatcagc gactcggtac cgagcaggct gaatgaaggc
     2641 gtgtaggcgt tgcccatgtc gccgccgggc atcaatttgc ccttgttgaa atggccttcc
     2701 gggtcgatct tctgcttgta ggcacggaag ggcgcaattt cttcctcgga gagaaattcc
     2761 agtttggtga tgccgatccc gtgctcaccg gaaatcacgc catccagcga gcgggcgagg
     2821 tgcatgatgc gctcgacggc tttatgggcc gtttgcaaca tgtcgtaatt gtcggaattg
     2881 accggaatgt tggtatgcac gttaccgtca ccggcgtgca tgtgcagggc gacaaaaaca
     2941 cggccgcgca gcacttcctt gtgaatggct tcaatgcgct cgacggtggg acgatagacg
     3001 ccaccatcaa aaatcttgct cagccgggcg tgaagttcct gtttccagga gacgcgtacc
     3061 gagtaatcct gcaggcggtg gaaaagacgc gggttttctg cccggttggt cagctcaccg
     3121 gcttgaaccg cataggtcgg gaagtgcaat tcagcctcag ccagcggcat gtcgaggctg
     3181 gccagcagcc atgcccagcg cagacgaacg gtctcgacat aatcgatcgc ggcttgccgg
     3241 cgatcaccga tcaagacctt ggtatcgagc gtcgtatcac cccgatgcag cggcaggtcg
     3301 cctttgagga actcaagcaa ggagtcgcac agcgccagct tgttgctgat cgacagctca
     3361 atgttgatgc gctcgatacc gtcgcaatag tcgcccatcc gcggtagcgg aatcacgacg
     3421 tcttcattga ccttgaacgc gttggtgtgg cgtgaaatgg cggcagtacg cgaacggtcg
     3481 agccagaatt tcttgcgggt ttcagcgtcg accgcaataa agccctcggc ggcgcgcaga
     3541 ttacacatgc gaacgacttc cgaggcggcc a
//
LOCUS       DAORPM010000247         3544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8678_length_3544_cov_13.014617, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000247 DAORPM010000000
VERSION     DAORPM010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8678_length_3544_cov_13.014617"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLD53_10485"
     CDS             <1..862
                     /locus_tag="PLD53_10485"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992481.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRC61386.1"
                     /translation="NIYNIAVRGMFDDAQDIVKAVSNDHAFKEKYKIGAVNSINWARV
                     VAQVVYYFKGYFAATQSNAEQVDFAVPSGNFGNICAGHIARMMGLPIARLVMATNEND
                     VLDEFFHTGVYRPRSTAETQATSSPSMDISKASNFERFVFDLVGRDPAIIRQLWTKID
                     SGGSFDLSSMPCFANLPQFGFVSGKSTHADRLRTISQTFEKYGVMVDTHTADGLKVAV
                     EQRVPDRSMVVLETALPAKFEETIIEALGRRPERPASFQGIENLPQRVEVMDVSVDAV
                     KRYIVQHVGA"
     gene            complement(953..1090)
                     /locus_tag="PLD53_10490"
     CDS             complement(953..1090)
                     /locus_tag="PLD53_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61387.1"
                     /translation="MLFALFYVVAITILVMHFTGFLARHNLEWLVLVLAAAVFPAVIF
                     L"
     gene            complement(1212..2090)
                     /gene="nadC"
                     /locus_tag="PLD53_10495"
     CDS             complement(1212..2090)
                     /gene="nadC"
                     /locus_tag="PLD53_10495"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRC61388.1"
                     /translation="MNTPSSSAPANPLDDEIERNVVAALAEDVGSGDLTARLVPADIV
                     TRATVVARENAVLCGTAWFERCFARLDPGISITWQAADGDHAVPDQILCTIEGPARAL
                     LTGERTALNFLQLLSGVATRANQYARAVAGTRAQVVDTRKTIPGLRLAQKYAVKCGGC
                     GNHRLGLYDAILIKENHILAAGSIASAMAAAKQVSDSTVSPCKFIQVEVESLDELGQA
                     LDAGAKMILLDNMNLDQMRAAVALNAGRAILEASGNVNLDTVRGIAETGVDRISVGGL
                     TKDVRAMDLSMRFQDQ"
     gene            complement(2174..2914)
                     /locus_tag="PLD53_10500"
     CDS             complement(2174..2914)
                     /locus_tag="PLD53_10500"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRC61389.1"
                     /translation="MIHFISDLHLSSHAPGVARIFLDYAGCRARTAEHLYILGDLFDV
                     WPGDDSIDDPDDTFNLAIVNALRELTDAGIGLSVMHGNRDFLLGEEFAARSGARLLPD
                     PYLLSLPAWQFILSHGDSLCIDDTDYQAFREQVRSPAWRTAFLQQPLAERKAIAASLR
                     QQSEQAKRQKKTQAQYPMDLNPGATDDFIRQHGYATFIHGHTHRPATHDHIVDGIHVE
                     RWVMSDWHEDCGEYLCWDGHHLNREVLR"
     gene            complement(2945..3436)
                     /locus_tag="PLD53_10505"
     CDS             complement(2945..3436)
                     /locus_tag="PLD53_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRC61390.1"
                     /translation="MIKLHTNFGVISLELDAEKAPESTKNFIAYAESGHYDNTVFHRV
                     IPGFMIQGGGFEPGMKQKPCKDPIKNEADNGVKNDTYTVAMARTSDPHSATAQFFINV
                     ADNEFLNFKAANANGWGYCVFGKVVDGTEVVDKIKGVRTGSSGFHQDVPKEDVIIERA
                     EVC"
BASE COUNT          739 a          952 c         1058 g          795 t
ORIGIN      
        1 caatatctac aatattgccg tgcgcggcat gtttgacgac gcgcaggata tcgtcaaggc
       61 ggtatcgaac gaccacgcct tcaaggagaa atacaagatc ggtgcggtca attcgatcaa
      121 ctgggcgcgt gttgtggcac aggtcgtcta ttacttcaaa ggctacttcg ctgcgacgca
      181 atccaatgcc gagcaggtcg actttgccgt gccttctggc aattttggca atatctgcgc
      241 cgggcatatt gcccgcatga tgggcttgcc gattgcgagg ctggtgatgg cgaccaatga
      301 gaatgacgtc ctcgacgagt tcttccatac cggggtttac cgtccgcgca gtacggctga
      361 aacccaggcc acttcgagtc cgtcgatgga tatttccaag gcctccaact tcgagcgctt
      421 tgtttttgac ctcgtcggcc gcgatccggc gatcattcgc cagttgtgga cgaaaatcga
      481 ttcgggtggc agcttcgatc tatcaagcat gccgtgtttc gccaatcttc cccagttcgg
      541 ttttgtgtca ggaaaaagca cccatgccga ccgtctacgg acgatcagtc aaacctttga
      601 gaaatacggg gtaatggtcg acacgcatac cgcagacgga ctcaaggtgg cggtcgagca
      661 gcgcgttcct gacagatcaa tggtcgttct agaaacggct ttgccggcca aatttgaaga
      721 aaccattatc gaggcgcttg ggcgccgccc ggaacgtccc gcaagttttc agggcattga
      781 aaatctgccg cagcgggttg aggtgatgga tgtcagcgtc gatgcggtga agcgctatat
      841 cgtgcagcac gtgggcgcct gatcgttcgg gatcgtcggc ctttgaatgg caagctaaac
      901 tcgggtgggg ctttttgcgg catttgaatc gtgcatccga atgctgaatc gcctatagaa
      961 agataacggc cgggaagacc gcggcagcaa gcaccaggac cagccattcc agattatgcc
     1021 tcgccagaaa cccggtgaaa tgcatgacca gaatagtgat ggctaccaca tagaacagcg
     1081 caaaaagcat gggacgcccc tttctttctt cgttcagccg gaatgtaccc gctgcggaat
     1141 tttttcttcc ggcaattata gcggcatcat ttgccagggg cgttattttt tccagagcat
     1201 gcgctgacca attactgatc ctggaagcgc atcgaaaggt ccattgcgcg cacatccttg
     1261 gtcaagccgc ccaccgagat gcggtcgacg ccggtttctg caatcccgcg aacagtatcg
     1321 agattgacgt tgcctgaggc ctccagtatt gcgcggccgg cattcaatgc gacggcagcg
     1381 cgcatctgat cgagattcat gttgtcgagc agaatcattt ttgcgccggc gtcaagcgcc
     1441 tgacccagct catccagcga ttcgacctca acctgaatga atttgcacgg gctgacggtg
     1501 ctgtctgaca cctgttttgc tgcggccatg gccgatgcga tgcttccggc agcaaggatg
     1561 tggttctcct tgatcagaat ggcgtcgtag agccccagac ggtgattgcc gcatccaccg
     1621 cacttgacgg catacttctg tgcgaggcgc agtccgggaa tcgtcttgcg ggtgtccaca
     1681 acctgagcac gggtgccggc aacggcacgg gcgtattggt tggccctggt cgccacgccg
     1741 gaaagcagtt gcaggaagtt gagtgccgtg cgctcgcccg tgagcagtgc gcgcgccggg
     1801 ccttcgatgg tgcacagtat ctggtcaggg acggcgtgat caccgtcagc ggcttgccaa
     1861 gtgatcgaga tgccagggtc gagcctcgcg aaacagcgtt caaaccaagc cgttccgcac
     1921 aacactgcat tttcgcgtgc gaccaccgtc gcgcgtgtaa cgatatcggc cggaacaagg
     1981 cgagccgtta gatcaccgct gccgacatcc tcggccagtg cagcaacgac gttgcgctcg
     2041 atttcatcat caagcgggtt ggcgggtgcc gacgacgaag gagtgttcat ctgagatcca
     2101 aagccttgaa atgcggaatg cagaattcta gcatcgggtg aagcatatgc ctgtgcttgc
     2161 gttcaatgga aagctagcgc agcacttccc ggttgaggtg atgtccatcc cagcacaggt
     2221 actcgccaca atcttcatgc cagtccgaca tcacccagcg ctcgacatgg atgccgtcaa
     2281 cgatgtgatc atgagtcgcc gggcggtgtg tgtggccgtg aatgaaggtg gcgtaaccgt
     2341 gctggcggat aaagtcgtcc gttgcgccag gattcagatc catcgggtac tgcgcctgag
     2401 tctttttttg gcgcttggcc tgctcgcttt gctggcgtag cgaagcggcg atggccttgc
     2461 gttcggccag tggctgttgc agaaacgcag tacgccaggc tggcgagcgc acctgttcgc
     2521 gaaaggcctg atagtccgtg tcatcaatac acaggctgtc accgtgtgaa aggataaact
     2581 gccaggcggg cagcgacagc aggtaggggt ctggtagaag ccgggcaccg cttcgtgcgg
     2641 caaactcttc gcccagcagg aaatcgcgat tcccgtgcat cacggagagg ccgatgcccg
     2701 cgtcggtcag ttcgcgcagg gcattgacga tggcaagatt gaacgtgtcg tccggatcgt
     2761 cgatgctgtc gtcgcccggc cagacgtcga agaggtcgcc gaggatgtag aggtgttcgg
     2821 ccgtacgcgc gcgacagccc gcgtagtcca gaaagattcg tgctacgccg ggtgcgtgtg
     2881 aggaaaggtg caggtctgaa atgaaatgaa tcacgtcaat tcagaccgga aatacgtgta
     2941 gtggtcagca gacttcagcg cgctcgatga tgacatcttc cttcggaaca tcctggtgaa
     3001 agcccgaact tccggtcctg acgcccttga tcttatcgac cacctcggtg ccatcgacca
     3061 ctttgccgaa tacacagtaa ccccaaccgt tggcattggc cgccttgaag ttcaggaatt
     3121 cgttgtcggc aacattgatg aagaactgtg cggtcgcaga gtgtgggtcg gaggtgcgcg
     3181 ccatggcaac cgtataggtg tcgttcttga cgccgttgtc tgcttcgttc ttgatcggat
     3241 ccttgcacgg tttctgtttc atgcctggct cgaaaccgcc gccttgaatc atgaagccag
     3301 gaatgacgcg gtggaatacg gtgttgtcgt agtgaccgga ttcggcgtag gcgatgaaat
     3361 tcttcgttga ttccggggct ttttcagcgt cgagttcaag agagatgacg ccgaaattag
     3421 tgtgcaactt gatcatgtgg cttccttact ttttgtctgg gatgatggtg acggactgga
     3481 tgacgacagg gtccagcggc acgttctgat gcccgctacg gttgccggtc gggaccttgg
     3541 cgat
//
LOCUS       DAORPM010000248         3522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8762_length_3522_cov_14.425151, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000248 DAORPM010000000
VERSION     DAORPM010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8762_length_3522_cov_14.425151"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..682
                     /gene="dksA"
                     /locus_tag="PLD53_10510"
     CDS             257..682
                     /gene="dksA"
                     /locus_tag="PLD53_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HRC61391.1"
                     /translation="MPEDISHKHFAPYVAKKGEEYMSKRQLAHFRTILETLKAELMDD
                     IERTVHTMQDEATVFADPNDRASQETDIAIELRNRDRERKLIKKIEETIDHIESGDYG
                     YCNGCGVEIGIKRLEARPTATLCIDCKTLEEVRERQIAK"
     gene            complement(762..2879)
                     /gene="pnp"
                     /locus_tag="PLD53_10515"
     CDS             complement(762..2879)
                     /gene="pnp"
                     /locus_tag="PLD53_10515"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRC61392.1"
                     /translation="MFTIARKSFTYGAHQVTLETGEIARQAGAAIMVTVDDTVVLVSV
                     VGNKSVKPGQDFFPLTVDYIEKTYAAGRIPGGFFKREGRPSEKETLTSRLIDRPLRPL
                     FPEGFYNEVQVVAMVVSLNPEIDPDIPAIIGASAAMAVSGIPFDGPIGAARVGYIDGQ
                     YVLNPSLTQLKSSQLNLVVAGTQSAVLMVESEADVLPEDVMLGAVVFGHEQMQVVINA
                     INELVDEAGKPEWNWAPAAKNEVLVGKLNDLVEADLQEAFRITSKQQRTHRIHEISEK
                     AVAVLTASGDAPDANTVKNLFFDVEAKIVRGRILAGEPRIDGRDTRTVRPIVIRSSVL
                     PRTHGSALFTRGETQALVVATLGTGRDEQIIDALAGEFRDRFMLHYNFPPYATGECGR
                     VGTPKRREIGHGRLAKRAMIAVLPNAEDFSYTMRVVSEITESNGSSSMASVCGSSLAL
                     MDAGVPLKAHVAGIAMGLIKDGNRFAVLTDILGDEDHLGDMDFKVAGTDTGITALQMD
                     IKIQGITKEIMHVALAQAKEARLHILGQMQSSVSGHREEVSTYAPRLYTMKINPEKIR
                     DVIGKGGAVIRAITEETGTTIDIKEDGSITIASISGEAANAAKSRIEAITADVEVGKV
                     YEGTVLKLLDFGAIVSILPGRDGLLHISQIANERVNAVVDYLKEGQKVRVKVLEADEK
                     GRVRLSMKALTAEEGGVASAAAQ"
     gene            complement(3060..3329)
                     /gene="rpsO"
                     /locus_tag="PLD53_10520"
     CDS             complement(3060..3329)
                     /gene="rpsO"
                     /locus_tag="PLD53_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRC61393.1"
                     /translation="MAINVAQKAQIMSDYQRAAGDTGSSEVQVALLTARINDLTGHFK
                     EHVKDHHSRRGLLRMVSRRRKLLDYLKGTNVDSYRALIQRLGLRK"
BASE COUNT          852 a          998 c          923 g          749 t
ORIGIN      
        1 cagatgcgct accaggctgc gctacgcccc gaagaagcgc gcattctaca gactgcccct
       61 tttaaggtca atcgccaatc actgattaag tagaggcaca aaaacctgcc acagttgtag
      121 cacagcgcgc tgggtacgat acaatccccc tgatcgatgt agtgtgagct cccggcattt
      181 tgtggatagc ttgcagcgtt tggcgatttc tgatatctaa tcgccttttt gcaaaacgac
      241 tggacggttt ctgaacatgc ctgaagatat aagccataaa catttcgccc cttatgtagc
      301 caaaaagggt gaggaataca tgagcaaaag acaattggct catttccgaa ccatcctcga
      361 aacgctcaag gccgaactga tggacgacat tgagcgtacg gttcacacca tgcaggatga
      421 ggcaactgtt ttcgctgatc ccaatgaccg cgcaagtcag gaaaccgaca tcgcgatcga
      481 gcttcgcaat cgtgaccgcg aacgcaagct gatcaagaag attgaagaga cgatcgatca
      541 catcgagagt ggtgactatg gctactgcaa cggttgcggc gtcgaaatcg gcatcaagcg
      601 ccttgaggca cgtccgaccg ctactttatg catcgactgc aaaacgcttg aggaagtccg
      661 cgaaagacag atcgccaaat agcctgtagt cacaacggca tgaacatgta aaaaggacgc
      721 cgaagcgtcc ttttcttttg cgcagccaag ttttctatgg gctattgcgc ggcagcgcta
      781 gccactccgc cttcttcagc agttagcgcc ttcatcgaca gacgcacacg ccccttttcg
      841 tccgcctcaa gcaccttgac gcggactttc tgaccttcct tgaggtaatc gacgacggca
      901 ttgacacgct cgttggcgat ctgggaaatg tgcagcaggc catcgcgacc gggcaaaata
      961 ctgacgatgg caccaaagtc gagcagcttg agcacggtgc cttcgtagac cttgcccact
     1021 tcaacatcgg cagtaatcgc ttcgatacgt gatttggccg cattggctgc ttcaccgctg
     1081 atcgaagcta tggttatcga accatcttcc ttgatgtcga tcgtggtgcc ggtttcttcg
     1141 gtgatcgcgc gaatgactgc gccacccttg ccgatcacat cgcgaatctt ctcggggttg
     1201 atcttcatcg tgtagaggcg cggcgcgtac gtcgacactt cttcgcgatg gccggagact
     1261 gagctctgca tctggccaag gatatgcaag cgagcctctt tggcctgcgc cagtgcaaca
     1321 tgcatgattt ccttggtgat gccctgaatc ttgatgtcca tttgcagggc ggtaatcccg
     1381 gtatcggtac cggcaacctt gaagtccata tcgccgaggt gatcctcatc gccgagaata
     1441 tcggtcagaa cggcgaaacg gttgccatcc ttgatcaggc ccatagcaat accagcaacg
     1501 tgcgctttga gtggcacacc ggcatccatc aatgccagcg atgaaccgca aaccgatgcc
     1561 attgagctgg acccgttgga ttcggtaatt tcggagacga cacgcatggt gtaggaaaaa
     1621 tcttccgcat tcggcagtac ggcaatcatc gcccgcttgg ccagacgacc atggccgatt
     1681 tcacgacgct tgggtgtacc tacgcggcca cattcgcccg tagcataggg aggaaagttg
     1741 taatgaagca tgaagcgatc acggaactca ccggcaagag catcaatgat ctgttcatca
     1801 cggcctgtac ccagcgttgc cacaaccagt gcctgtgttt cgccacgcgt gaaaagggcc
     1861 gaaccgtgcg tacgaggtag gacgctcgaa cgtatgacaa tcggcctaac cgtgcgcgta
     1921 tcacgaccgt cgatacgggg ttcgccagca agaatgcggc cacgcactat ctttgcctca
     1981 acgtcgaaga acaggttctt gacggtgttg gcatccggag cgtcgccact agccgttagc
     2041 acggcaactg ccttctcgga aatctcatga atgcgatgcg tacgctgctg tttgctggta
     2101 atgcggaaag cttcctgcag atcggcttcg accagatcat tgagtttgcc gaccagcact
     2161 tcattcttgg cggcaggcgc ccagttccat tccggcttgc cggcttcgtc gaccaactcg
     2221 ttgatggcgt tgatgacaac ctgcatttgc tcgtggccaa agacgacagc accgagcatg
     2281 acatcctcgg gcaacacgtc ggcctccgat tcaaccatca acaccgccga ctgagtaccg
     2341 gcaacgacga ggttgagctg gctgctcttg agttgtgtca gcgacggatt gagcacatat
     2401 tggccatcga tatacccgac acgagcggca ccgatcggac catcaaaggg gatgcccgat
     2461 acagccatgg ccgcagaagc accgataatg gccgggatat ccgggtcaat ctcggggttg
     2521 agcgagacca ccatcgcaac aacctgcact tcgttataga aaccttccgg gaagagcgga
     2581 cgcagcggac gatcgatcag acgcgaggtt agtgtttcct tttccgacgg gcggccttcg
     2641 cgcttgaaga aaccaccggg aatccggcca gccgcatagg tcttttcaat gtaatcaaca
     2701 gtcagcggga aaaaatcctg ccctggcttg accgacttgt tgccaaccac cgagaccaga
     2761 acgacggtat catcaaccgt caccatgatg gccgcaccgg cctgacgcgc tatctcaccg
     2821 gtttcgagtg tcacctggtg agcaccatac gtaaaagatt tcctggcgat agtaaacatt
     2881 atttcctctc tcaatagacc aacgcgcgca cccacgacac cggaacgcaa ttcaacattg
     2941 agccaacatg gcgaaatgct gcagaaacaa caacagcggc atcgcccgca aagggctggc
     3001 cgctgtcatc ccaacaaatg atctgcaccc ccaaccgcac agcggttcgg caggcaggct
     3061 tatttacgca ggccgagacg ctgaatcagg gcacgatagg aatcaacatt cgtgcccttc
     3121 aggtagtcca gcaacttgcg tcgacgactc accatgcgca acagaccgcg acgggagtga
     3181 tgatccttga catgctcttt gaagtgaccg gtcaggtcgt tgatgcgtgc ggtgagcagt
     3241 gccacttgga cttcggagga accggtgtcg cctgcggcac gctgataatc actcatgatt
     3301 tgcgcttttt gcgctacatt gatcgccatt tcaaactctc tttcgtgaat tgcgacaccg
     3361 ccccagtttg atagagcaaa tgccagttaa ggaatgtgtt actcgctcgg cgaagcgagc
     3421 tgcggattat agtctgttcg acggcatacg ggcaagcacg caagatcagc acgcaagatc
     3481 gaggcaataa gcgaaacaga accgattgcc actatcaggc ac
//
LOCUS       DAORPM010000249         3518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8781_length_3518_cov_10.220907, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000249 DAORPM010000000
VERSION     DAORPM010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8781_length_3518_cov_10.220907"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLD53_10525"
     CDS             <1..540
                     /locus_tag="PLD53_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRC61394.1"
                     /translation="FDLLYWNSDSTRMPATMHSFYLRNFYLGNKLREPGGIELGGAKI
                     DISKVKTPCYFVSAIEDHIAPWKSTYMGALLPTGPVKFVLGGSGHIAGIINPPVANKY
                     GFWTNAEMPASSDDFLAGATQNPGSWWTDWQQWVTDHAGGKEKIDARKPGDGPLKAIE
                     DAPGSFVKLRLDKKKVAAK"
     gene            608..1801
                     /locus_tag="PLD53_10530"
     CDS             608..1801
                     /locus_tag="PLD53_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRC61395.1"
                     /translation="MSSTRSTDPIGTTRLPDYAGNSIVNLMQSIATACGSTQRRYHQL
                     QALPAAKLGEARHIVLLVIDGLGQRTLERHPASPNLQRHAIGTMTSVFPSTTASAITT
                     FMTGLAPAQHGLTGWHMHMDEIDQTLAILPLTPRCAPAKFLPEELPSRLFDHQSLFQQ
                     LDRDCWVIAPLDIVGSPFNAWHSRGARSLAYNTIPEMFAQLSTLLREAETPRYIYAYY
                     PYLDSHSHSFGCESKQALLNLAALDTLFGNLLLDAKGSDTWLIATADHGFIDSPPERV
                     ISLDQHPQLAALLTRPLCGERRATYCYVSEDRRPAFESYVRQHLDHCVDLHRSSDLID
                     AGWFGPPPYHPRLASRIGDYTLVMKDDWTIKDRLPGERHYPMLGVHGGISGAEMTVPL
                     IAVRA"
     gene            1885..2274
                     /locus_tag="PLD53_10535"
     CDS             1885..2274
                     /locus_tag="PLD53_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HRC61396.1"
                     /translation="MPPNKPLGSRLDQVVADAIRNRQSREAGYREQALKIYPWICGRC
                     AREFVRENLQQLTVHHKDHNHDNNPPDGSNWELLCLYCHDNEHSRERDAEAARQAGAG
                     LGTKAEVNRATAQPFANLKDLLSKKQQ"
     gene            2335..3315
                     /locus_tag="PLD53_10540"
     CDS             2335..3315
                     /locus_tag="PLD53_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC61397.1"
                     /translation="MNKLIRHVVAFGLAVLMGFAPLAQAEAPQVQTQVPGYYRAQLGK
                     FEITALYDGMIELDTKLLKNTSTVELQVLLARMFVGNPKMQTAVNAYLINTGSHLVLV
                     DTGAAKLFGPSLGYVLQNMKASGYEPAQVDMVIITHLHADHVGGLNDADGKPLFPKAK
                     ILVAQEDGDYWLSQTVADGAPKEVQPFFKMARDSAAPYLSSGQWSTFTQGSVLVPGIK
                     AVKANGHTPGHTAFAVESEGQKLLIWGDLVHAHAVQFARPGVSIEFDIDQKQAIATRR
                     SIMKDMASRKSLVAGMHLPFPGLGHVRADSKGRYSWVPIEFAPMPKPAGQ"
     gene            3374..>3518
                     /locus_tag="PLD53_10545"
     CDS             3374..>3518
                     /locus_tag="PLD53_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HRC61398.1"
                     /translation="MPSLKALAPILFVFLWSTGFIGAKFGLPYADPLTFLLARYILVL
                     CLMT"
BASE COUNT          732 a         1088 c          997 g          701 t
ORIGIN      
        1 ttcgatctgc tctactggaa ttccgactcg acgcgcatgc cggcgacgat gcacagtttc
       61 tacctgcgca atttctatct gggcaacaag ctgcgcgagc cgggtggcat cgagctgggc
      121 ggcgccaaga tcgacatctc caaggtcaag acgccgtgct acttcgtttc ggcgattgaa
      181 gaccacattg ccccgtggaa gagcacgtac atgggcgcat tgcttcccac cggaccggtg
      241 aagttcgtcc ttggcggttc cgggcacatt gccggcatca tcaatcctcc ggtagccaat
      301 aaatacggct tctggaccaa cgccgaaatg ccggcatcgt ccgacgattt cctggccggc
      361 gcgacgcaga acccgggttc atggtggacg gactggcagc agtgggtgac cgatcatgcc
      421 ggcggcaagg agaagattga cgcgcgcaaa ccgggtgacg gccccctgaa ggcgattgaa
      481 gacgcacctg gttccttcgt caagttgagg ctggacaaga agaaagtcgc cgccaagtaa
      541 tctgcggtct gcttgaatca tcaagggggc gcaagccccc ttttccgttg acacacaatc
      601 gattttcatg agttcgactc gttctactga tcctattggc accactcgct tgccggacta
      661 tgccggcaac agcatcgtca acctgatgca gagtattgcc acggcttgtg gcagtaccca
      721 aaggcgatac catcaactgc aggccttgcc cgctgcaaag ctgggcgagg ctcgccacat
      781 cgtcctgctg gtgatcgacg gcctcgggca gcgcacgctg gaacgacacc cggccagtcc
      841 gaatcttcag cgtcacgcaa tcggcacaat gacatcggtg tttccgtcga ccaccgccag
      901 cgccatcacc accttcatga ccgggcttgc cccggcgcaa cacggcctca caggctggca
      961 catgcacatg gacgaaatcg accagacgct ggccatcctg ccgctgacac cgcgttgcgc
     1021 gcccgcgaaa ttcctgccgg aagagctgcc gtcgaggctt ttcgaccatc agtcactctt
     1081 ccagcagctt gaccgtgact gctgggtcat cgcacccctg gacatcgtcg gcagtccctt
     1141 caacgcctgg cattcacggg gcgcccgcag tctggcctac aacacgatac cggagatgtt
     1201 tgcgcagctt tccactctgc tacgagaggc cgaaacgccg cgctacatct acgcctacta
     1261 cccttatctc gattcgcatt cacacagttt cggctgcgaa agcaaacagg ctttgctgaa
     1321 tctggccgca cttgacacgc tgttcggcaa tctgctgctc gatgcaaagg gcagcgacac
     1381 ctggctgatc gccactgccg accacggttt catcgattca ccacccgagc gtgtgatcag
     1441 tctcgaccag catccgcagc tcgccgcact actcacccgc ccgctgtgcg gcgaaagacg
     1501 cgctacctac tgttacgtca gcgaggatcg tcgcccggcg ttcgaaagct acgtgcggca
     1561 acacctggat cactgtgttg atttgcaccg aagcagcgat ctaatcgacg ccggctggtt
     1621 cgggccgccg ccctatcacc cgcgcctggc ctcgcgcatc ggcgactaca cgctggtgat
     1681 gaaggacgac tggacgatca aggacaggct ccccggcgag cgtcactatc cgatgctcgg
     1741 tgtgcatggc ggcatcagcg gcgccgaaat gacggtgccg ttgattgccg tccgggcgta
     1801 attcttcagg cttgtcagcg cacggcgcga tagccataat tcaacgtctg gagactcact
     1861 cccgccctcc tcggaacatc aggaatgcca ccgaacaagc cactaggttc ccgcctcgat
     1921 caggtcgttg ccgacgcgat tcgcaatcgt cagtcgcgcg aagccggcta tcgtgagcag
     1981 gcgctgaaga tctatccatg gatctgcgga cgttgcgcgc gagaattcgt gcgtgaaaac
     2041 ttgcagcagc ttaccgtgca tcacaaggat cacaaccacg acaacaaccc gccggatggc
     2101 agcaactggg aactgttgtg tctgtattgc cacgacaacg agcattcgcg ggaacgtgac
     2161 gccgaagcgg cacgccaggc gggagccggt ctcggcacca aggccgaggt gaatcgcgcc
     2221 accgcgcagc cctttgccaa tctgaaggat ttgctgagca aaaagcagca gtgagcacgg
     2281 cgttcttcgg ccaggcggta atcaaatcaa acaagtttta gaggagatag ttcgatgaac
     2341 aaacttatcc ggcatgttgt ggcctttggt ctggctgtcc tcatgggctt cgcgccgctc
     2401 gcccaggcag aagcaccgca ggttcaaacg caggtgcccg gctattaccg cgcacagctc
     2461 ggcaagttcg aaatcacggc gctttacgac gggatgatcg agcttgacac caagcttttg
     2521 aaaaacacca gtacggttga attgcaggtt ctgctggccc ggatgtttgt cggcaatccg
     2581 aagatgcaga cggcggtcaa tgcctatctg atcaacaccg gcagtcatct ggtgctggtc
     2641 gataccggtg ccgccaaact gtttggcccg tctttgggtt acgttctgca gaacatgaag
     2701 gcttccggct acgagcctgc ccaggtggat atggtcatca tcactcacct gcatgccgat
     2761 catgtgggcg ggctgaatga cgccgacggc aagccgctgt tcccgaaagc gaagattctg
     2821 gttgcccagg aggatggcga ttattggcta tcgcagacag tcgccgatgg tgcacccaag
     2881 gaagtccagc ccttcttcaa aatggcgcgc gacagtgcgg ccccgtattt gagcagcggg
     2941 caatggagca cctttacgca gggcagcgtg ctggttcccg gaatcaaggc cgtcaaggcc
     3001 aacggccata cgccgggcca caccgcgttt gccgttgagt ccgaaggaca gaaactgctg
     3061 atctgggggg atctggtgca cgcgcacgcg gtgcagttcg cgcggcccgg cgtttccatc
     3121 gagttcgaca tcgaccagaa acaggccatc gcaacccggc gcagcatcat gaaggatatg
     3181 gcttccagaa agtccctggt ggctggcatg catctgccat ttcctggcct cggacatgtc
     3241 cgggcggata gcaagggccg ttacagttgg gtgccgatcg agttcgcgcc gatgcccaag
     3301 ccagcaggtc aatgaatcaa agcgcccggt gacggacccg gtatagtgtc ggattttccc
     3361 gaatccgcac accatgccgt cccttaaagc gctcgctccc atcctcttcg tattcttgtg
     3421 gagcaccggt ttcattggcg ccaagttcgg cctgccctat gccgatccgc tgactttcct
     3481 tcttgcacgt tacattctgg tactgtgcct gatgacga
//
LOCUS       DAORPM010000250         3504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8840_length_3504_cov_16.334300, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000250 DAORPM010000000
VERSION     DAORPM010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8840_length_3504_cov_16.334300"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLD53_10550"
     CDS             <1..1002
                     /locus_tag="PLD53_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRC61399.1"
                     /translation="VRHPSEVLNVGDEVTAKILKFDAEKNRVSLGMKQLGDDPWVGIA
                     RRYPAGTRLFGKVTNLTDYGSFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVSLGDEVE
                     VMILEIDEERRRISLGMKQCQANPWDDFAMNHKKGDKVKGAIKSITDFGIFIGLPGGI
                     DGLVHLSDLSWSATGEEAIRNFKKGDEVEALVLAIDVEKERISLGIKQMEGDPYTNFI
                     ATHEKNSIVRATVKTVDAKGAVLTLDGENEGYLRASEFSRDRIDDLSQHLKVGDVVEA
                     MIINVDRKTRGINLSIKAKDNAEQNEAMQKFSADSNSAASGTTNLGALLKAKLNQQG"
     gene            1015..1299
                     /locus_tag="PLD53_10555"
     CDS             1015..1299
                     /locus_tag="PLD53_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HRC61400.1"
                     /translation="MTKSELIARLAERFPQLVAKDADFAVKVILDAMSEALVRGDRIE
                     IRGFGSFALNYRPPRTGRNPKSGEKVSVPAKWVPHFKAGKELRERVDQSI"
     gene            1368..1649
                     /locus_tag="PLD53_10560"
     CDS             1368..1649
                     /locus_tag="PLD53_10560"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRC61401.1"
                     /translation="MTFLTWALRFIIFFVLLAFALRNTQPVSLQLILDHAWEAPLVIV
                     LLVFFACGAILGILSVVGVVFRQRREISRLKREAVKPQQPVFPEPSAPI"
     gene            1646..2818
                     /gene="lapB"
                     /locus_tag="PLD53_10565"
     CDS             1646..2818
                     /gene="lapB"
                     /locus_tag="PLD53_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HRC61402.1"
                     /translation="MNELFEYWQLLLIPVFFLLGWAAARVDMRQVVHESRALPRSYFQ
                     GLNFLLNEQPDKAIDSFLEVAKVDSQTVELHFALGNLFRRRGETERAIRMHQNLIDRP
                     DLDDGVRLHALSELGQDYLKAGLLDRAEEIFNKLIGTPFEDEAKRSLLEIFQAEKEWL
                     KAIELARELPDVASQQEVAEFYCELAANEIMRSKPEPARAYLELAMQQNRKCVRASLL
                     QGDLLLQEGRQEAAIEAWQRIEQQDPAYLALIAQRLLESYRKLERRDEGIALLSVYLE
                     RYPSLDLLDVVYQLVLEGEGTEAAYRLVRAELQRNPTLLGLEKLMSARLPLVAPDVRP
                     DVELAKTIIQGYTKRLSRYRCDNCGFKARQFYWRCPACGGWETYPPRRSEEFDQTL"
     gene            2828..>3504
                     /locus_tag="PLD53_10570"
     CDS             2828..>3504
                     /locus_tag="PLD53_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRC61403.1"
                     /translation="MKITVVGTGYVGLVSGTCLAEVGNDVLCLDVDPAKIRILEEGGI
                     PIYEPGLQEMVRRNVAAGRLHFTTDIEKAVQHGTIQFIAVGTPPDEDGSADLQYVLAA
                     ARSIGRLMTDYKVVVDKSTVPVGTGAKVKAAILDELAKRAVDIPFSVVSNPEFLKEGA
                     AVEDFMRPDRIVVGAEEEQAIHLMRALYAPFQRNHERLIITDVKSAELTKYAANAMLA
                     TRISFMN"
BASE COUNT          755 a          939 c          989 g          821 t
ORIGIN      
        1 gtccgtcacc cgagcgaagt gctcaatgtc ggtgacgaag ttactgccaa gattctcaag
       61 tttgacgctg agaagaatcg cgtctccttg ggtatgaagc aactgggcga cgatccgtgg
      121 gttggtattg cccgccgtta cccggctggt acccgcctgt tcggcaaggt taccaacctg
      181 accgactacg gttcattcgt tgaaatcgaa caaggcatcg aaggtctggt tcacgtttcc
      241 gaaatggatt ggaccaacaa gaacgttcat ccgtccaagg ttgtttcctt gggtgatgaa
      301 gtcgaagtca tgatccttga aatcgacgaa gagcgtcgtc gtatctcctt gggtatgaag
      361 cagtgccaag ccaatccttg ggacgacttc gcgatgaacc acaagaaggg cgacaaagtt
      421 aagggcgcca tcaagtcgat caccgacttt ggtatcttta tcggcttgcc gggtggtatc
      481 gatggtctgg ttcacttgtc cgacctgtcc tggagcgcaa ctggcgaaga agccatccgc
      541 aacttcaaga agggtgacga agttgaagcc ctggttctcg ccatcgacgt cgagaaagag
      601 cgtatctccc tcggcatcaa acagatggaa ggtgatccgt acaccaactt catcgctacc
      661 cacgagaaga acagcatcgt ccgcgctacc gtcaagacgg ttgatgctaa gggtgccgtg
      721 ctgacgctgg atggtgagaa cgagggttac ctgcgtgctt ccgagttctc gcgtgatcgt
      781 atcgacgacc tgtcgcaaca cctgaaggtc ggtgatgtgg ttgaggcgat gatcatcaac
      841 gtggatcgca agacccgtgg tatcaatctg tcgatcaaag ccaaggacaa tgccgaacag
      901 aatgaagcca tgcagaaatt ctctgctgac tccaactctg ctgcttctgg cacgaccaac
      961 ctgggtgccc tgctcaaagc caagctcaac cagcaaggct gataggcata aagcatgacc
     1021 aaatccgagc tcatcgcccg gctggcggag cggtttccgc agttggtggc gaaagatgct
     1081 gattttgcgg tgaaagtgat tctcgatgcg atgtccgaag ccttagtgcg cggcgatcgt
     1141 atcgagatcc gcggattcgg cagctttgcg ctcaactacc ggccgcctcg cactggccgt
     1201 aaccccaagt cgggggagaa ggtcagcgta cctgccaaat gggttcccca tttcaaggcg
     1261 ggcaaggaac tgcgcgaacg ggtcgatcag tcgatctgac gctgcgcagg cgctatgtgg
     1321 tagattcggg gcagtgaatt tcactgcccc tttttctttt tgaagccatg acttttctga
     1381 cttgggccct tcggttcatt attttctttg ttttgctcgc tttcgccttg cgcaacacgc
     1441 agccagttag cctccagttg attcttgatc atgcttggga ggcgccactg gttatcgttc
     1501 tcttggtgtt ttttgcgtgc ggcgcaattc tcggtatctt gtcggttgtt ggggtcgttt
     1561 ttcgccaacg ccgtgaaatt tcgcgcctca agcgtgaggc agtaaagccg cagcaaccgg
     1621 ttttcccgga accatcagcc ccgatatgaa cgaactcttt gaatattggc agctattact
     1681 tatcccagtt ttctttttat tgggttgggc tgctgctcgc gttgatatgc ggcaagtggt
     1741 tcatgagtcc cgcgccttgc cacgctccta ttttcagggg ctcaatttcc tgctcaacga
     1801 gcagcccgac aaggccatcg actcgtttct cgaagtggcg aaggtcgatt cccagaccgt
     1861 tgaattgcac tttgcgcttg gcaatctttt tcgccggcgt ggcgagactg agcgcgccat
     1921 ccgcatgcac cagaatctga tcgatcgtcc cgatctcgat gatggcgttc gtctgcatgc
     1981 gctctccgaa ttggggcagg attatctgaa ggccggtctg cttgaccggg ctgaggaaat
     2041 tttcaacaaa ctgattggta ccccgtttga agatgaagcc aagcgcagtc tgctggaaat
     2101 ttttcaggct gaaaaagagt ggctgaaggc gattgaactg gcgcgcgagt tgcccgatgt
     2161 ggcctcgcag caagaggtcg ccgagtttta ctgtgaattg gcagctaacg aaataatgcg
     2221 ctcgaagccg gaaccggctc gtgcttatct cgaattggcc atgcagcaaa atcgcaaatg
     2281 tgtgcgagcc agtctcttgc agggcgattt gttgctgcag gaaggccggc aggaagctgc
     2341 gatcgaagct tggcagcgta tcgaacagca ggatccagcc tacttggcgt taattgccca
     2401 acgcctgctt gagagctatc gcaagcttga gcgccgggac gaaggtatcg cgctgctcag
     2461 cgtttacctc gagcgctatc cgtcgcttga tctgttggat gtggtttatc aattggtgct
     2521 ggaaggtgaa ggaacggaag cggcttaccg attggtgcga gctgaactcc agcgcaatcc
     2581 gactctgctt ggcctggaaa aactgatgag cgcccgtctg cctttggtgg cgccggatgt
     2641 caggccggat gtcgaactgg ccaagacgat catccagggc tataccaagc gactgtcacg
     2701 ttatcggtgc gataattgtg gtttcaaggc gcgccaattt tactggcgct gtccggcctg
     2761 tggcggctgg gaaacctatc cgccgcgccg tagcgaagaa ttcgatcaaa ccttgtagga
     2821 aacttttatg aaaataaccg ttgtcggtac cggctatgtc ggtctggtaa gtggcacctg
     2881 tctggctgaa gtgggcaatg acgttctctg tctggacgtt gatccggcaa agattcgtat
     2941 tctcgaagag ggcggcatcc cgatctatga accgggcctg caggaaatgg ttcgccgcaa
     3001 cgtcgcggcc ggtcgtttgc atttcaccac cgacatcgaa aaagccgtac agcacggcac
     3061 tatccagttc atcgccgttg gtacgccgcc cgacgaagac ggctcggctg atctccagta
     3121 tgtgctcgcc gctgctcgca gcatcggccg cctgatgacc gattacaaag ttgtggtcga
     3181 caaaagtact gtcccggttg gtaccggtgc caaggtcaag gcagcgattc tcgacgaact
     3241 ggccaagcgt gccgttgaca tcccgttcag cgttgtttcc aacccggaat tcctgaaaga
     3301 aggcgccgcc gttgaagact tcatgcgtcc cgaccgcatc gtcgtcggcg cagaagaaga
     3361 gcaagccatc cacctgatgc gcgctctcta cgccccgttc cagcgcaacc acgaacgcct
     3421 gatcatcacc gacgtcaaga gcgccgaact caccaagtac gccgccaacg ccatgctggc
     3481 gacgcgcatc agcttcatga acga
//
LOCUS       DAORPM010000251         3503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8849_length_3503_cov_22.423724, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000251 DAORPM010000000
VERSION     DAORPM010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8849_length_3503_cov_22.423724"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PLD53_10575"
     CDS             complement(<1..1394)
                     /locus_tag="PLD53_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRC61404.1"
                     /translation="MMNQLYGTTPLFGGNAPFVEDLYENYLDNPASVPIEWREYFDKL
                     AQLPSAVARDVPHLPIVQAFAEQARLGGYRATLSKPVDDKKQVSVLQLITAYRTLGAR
                     WANLDPLKRQTRPVINELEPSHYGLGSADMNTLYNAGSFKGVGERATFGQIFDSLKET
                     YCSSIGIEYMYIDNIAEKRWLQSRIEPIHAKGTYTSAQKLRFLERLTAAETLERYLHT
                     RYVGQKRFSLEGGESLIVALDELVRVAGGLGVDDVVIGMAHRGRLNVLVNTLGKEPAM
                     LFDEFEGKKAQDLTSGDVKYHLGYSSDVSTPGGACHLTLAFNPSHLEIVNPVVIGMAY
                     ARQHRRGEGSQEKIFPVLVHGDAAVAGQGVNQEMLNFSQTRGYGTGGTVHIVVNNQIG
                     FTTSDPRDSRSSLYCTDIFKMVNAPIFHVNGDDPEAVALVTRLAVEFRHEFRKDVVID
                     IVCFRKLGHNEQDEP"
     gene            complement(1518..2813)
                     /gene="gltA"
                     /locus_tag="PLD53_10580"
     CDS             complement(1518..2813)
                     /gene="gltA"
                     /locus_tag="PLD53_10580"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRC61405.1"
                     /translation="MSTERKATLIIEGRDPVEFPIMTPVAGYDCIDIRTLGSKTGMFT
                     YDSGFLSTAACRSEVTYIDGDKGELLYRGYPIEQLATNCNFLDVAYLLKHGELPNANE
                     KIAFEATIKDHTMLHEQLVKFYQGFRRDAHPMAVLVGVVGALSAFYHEAEDFSDAEHR
                     NISYNRIIAKLPTIVAYAYKYHTGQPFMYPRNDLDYTSNFMHMMFGTPCETYKPNPVL
                     VHALDTIFTLHADHEQNASTSTVRLAGSSGANPYACVSAGIGCLWGPAHGGANEACLK
                     MLEEIGDVSRVPEFIKRAKDKDDPFKLMGFGHRVYKNFDPRATLMRKVCADVLKEQGL
                     ENDPLFKLAMELERIALEDQYFIDKKLYPNVDFYSGIVQKAIGIPTSMFTCVFALART
                     VGWMVQWEEMIADPEYKIGRPRQLYIGVPARDVPPLDKR"
     gene            complement(2849..3139)
                     /locus_tag="PLD53_10585"
     CDS             complement(2849..3139)
                     /locus_tag="PLD53_10585"
                     /inference="COORDINATES: protein motif:HMM:NF015870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HRC61406.1"
                     /translation="MEADAELNRLRWRCTRRAMLEMDLLLGDFLDTHFSGLTPEQAAA
                     FVVLADMEDLELWPLITGKRECANSLQAEVVSMLRNVRVKYSSGNAPTSGQA"
     gene            complement(3139..>3503)
                     /locus_tag="PLD53_10590"
     CDS             complement(3139..>3503)
                     /locus_tag="PLD53_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRC61407.1"
                     /translation="KPYLINDSPPPERERLQTPEDRDELNGLYECILCACCSTSCPSF
                     WWNPDKFVGPAGLLNAYRFIADTRDEATNARLDDLEDPYRLFRCHTIMNCVEVCPKNL
                     NPTQAIGKIKEMMVRRAI"
BASE COUNT          748 a          991 c          983 g          781 t
ORIGIN      
        1 ggctcgtcct gctcgttgtg gccgagcttg cggaaacaga cgatatcgat gacgacatcc
       61 ttcctgaatt catgcctgaa ttcgaccgcc agacgggtca ccagcgccac ggcttccgga
      121 tcgtcgccat tgacgtggaa aatcggcgca ttgaccatct tgaagatgtc ggtgcaatac
      181 agtgatgaac gcgaatcgcg tggatcggag gtggtgaacc cgatctggtt attgacgacg
      241 atatgcaccg ttccgcccgt accgtatccg cgagtctggg agaagttgag catttcctgg
      301 ttgacgcctt gaccggccac ggcggcatcc ccgtgaacca gcactggaaa tatcttttcc
      361 tgggaccctt cgccgcgccg atgctgacgc gcatacgcca taccgatgac aacagggttg
      421 acgatttcaa gatgcgacgg attgaaagcc agcgtcaaat ggcaggcgcc ccccggggtc
      481 gatacgtctg aagagtagcc taggtggtac ttgacgtcgc cggaggtcag atcctgcgct
      541 ttcttgcctt cgaactcgtc aaacagcatt gccggttcct tgcccagcgt attgaccaga
      601 acattcagac gtccacgatg cgccatgccg atcacgacgt cgtcgactcc taaaccaccg
      661 gcgactcgta ccagctcgtc cagcgcgacg attaaggact ccccgccctc aagcgagaac
      721 cgcttctgtc caacgtagcg ggtatgcaga tagcgttcga gtgtttcggc cgccgtcagg
      781 cgctcaagga agcgcagttt ctgggcggac gtatacgttc ccttggcgtg gatcggttcg
      841 atgcgcgact gcagccagcg tttttcggca atgttatcga tgtacatgta ttcgatgccg
      901 atcgagctgc aataggtctc cttgagggaa tcgaaaatct ggccgaaggt cgcgcgttcg
      961 ccaacgcctt tgaacgaacc agcgttgtac agggtgttca tgtcagcact gcccagtccg
     1021 tagtgcgacg gctccagctc attgatcacc ggtcgcgtct gtcgcttgag cggatcgaga
     1081 ttggcccaac gtgcacccag tgtgcggtaa gccgtaatca gttgcaggac actgacctgt
     1141 ttcttgtcgt cgactggctt ggacagtgtc gcgcgataac ctcccagtct ggcctgttcg
     1201 gcaaacgctt gcacaatcgg caagtgggga acatcgcggg caacagcgct aggcagttgc
     1261 gccagcttgt cgaagtattc gcgccactca atgggaaccg acgcgggatt gtcgagataa
     1321 ttttcgtaga gatcttccac gaatggggca ttgccgccga acagtggagt cgttccgtag
     1381 agctgattca tcatggcagt catcttttgt cgggattccc aaggtctgat tgaagaattt
     1441 ggccggaagc tttacctggc gaccagacga gaaaggcgac agccttcgct gtcgcctccc
     1501 tttgttgcta actctgctta acgcttgtcg agcggaggga catcgcgtgc gggaactccg
     1561 atataaagtt gacgcggacg accaatcttg tactccgggt cggcaatcat ttcttcccac
     1621 tgtaccatcc agccgaccgt tcgcgccagt gcaaagacgc aggtgaacat cgaggttgga
     1681 ataccaatgg ccttctgcac gatgcctgaa tagaaatcca cattcggata gagcttcttg
     1741 tcgatgaagt actggtcttc cagcgcaatc cgttccagct ccatggccag tttgaacagc
     1801 ggatcgtttt ccaggccctg ttccttcaac acgtcggcac agaccttgcg catcagcgtc
     1861 gcacgtggat cgaagttctt gtagacccgg tggccgaaac ccatcagttt gaaaggatcg
     1921 tccttgtcct tggcgcgctt gatgaactcc ggaacacgcg agacatcgcc gatctcttcg
     1981 agcatcttga ggcaggcttc gttggcaccg ccgtgcgctg gcccccacag acatccgatt
     2041 cccgccgaaa cgcaggcata cggattggcg cctgacgaac ccgctaggcg aacggtcgac
     2101 gtcgaggcat tctgctcgtg atcggcatgc agggtaaaaa tggtgtcgag tgcatgcacc
     2161 agcaccggat tgggtttgta ggtctcgcac ggtgtgccga acatcatgtg catgaaattg
     2221 ctggtgtagt ccagatcatt gcgcggatac atgaacggct ggccggtgtg gtacttgtaa
     2281 gcgtacgcaa cgatcgtcgg aagcttggcg atgatccggt tgtagctgat attgcgatgc
     2341 tcggcgtcgg agaagtcttc cgcttcgtga tagaacgcag acagggcacc aacgacaccg
     2401 accagaaccg ccatcggatg cgcgtcacga cggaagccct gatagaactt caccagttgc
     2461 tcgtgcaaca tggtgtggtc cttgatggtc gcttcgaagg ctattttctc attggcattt
     2521 ggcagctcgc cgtgcttcag cagataagct acatcgagaa agttgcagtt cgtcgccagt
     2581 tgctcgatcg gatagccgcg ataaagcaac tcgcccttgt caccatcaat ataggtgacc
     2641 tcggaccgac aagccgcggt ggataagaac ccggaatcgt aggtaaacat cccggtcttg
     2701 ctacccaggg ttcgaatatc aatacaatca tagccagcta ccggcgtcat gatcgggaat
     2761 tcgacggggt ctcgcccctc gataatcagg gtggctttgc gttcggtgct cataataatc
     2821 tccggttctg gttttagaag tcgtttgctc aagcttgccc cgatgtcggg gcatttccac
     2881 tactgtactt tactctgacg ttccgcaaca tcgacacgac ctcggcctgc agggagttcg
     2941 cacactcgcg cttaccggta atcaaaggcc aaagctccag atcctccata tcggccagta
     3001 ccacaaacgc cgccgcctgt tccggagtca ggccggagaa gtgcgtgtcg agaaagtctc
     3061 ccagcagcaa atccatctcc agcatggcgc ggcgcgtgca acgccagcgc aaccggttca
     3121 gctcggcgtc ggcctccatc aaatcgctcg cctgaccatc atttccttga tcttgccgat
     3181 ggcctgggtc ggattcaggt tcttcggaca gacctcaaca caattcatga tggtgtgaca
     3241 gcggaacagg cggtaagggt cctcaagatc gtcaagacgc gcgttggttg cctcatcacg
     3301 cgtgtcggca atgaagcggt aggcgttcag aagccctgct gggccaacga acttgtccgg
     3361 attccaccag aacgacgggc acgatgtcga gcaacaggcg cagagaatgc attcgtacag
     3421 accattgagt tcatcgcgat cctctggcgt ctgcagtcgc tcacgctccg gcggtggcga
     3481 gtcgttgatc aggtacggct tga
//
LOCUS       DAORPM010000252         3489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8900_length_3489_cov_10.297030, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000252 DAORPM010000000
VERSION     DAORPM010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8900_length_3489_cov_10.297030"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLD53_10595"
     CDS             <1..864
                     /locus_tag="PLD53_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRC61408.1"
                     /translation="ERTGNVDLVTLALNLYTQGIAPNLDFSDINAVARNFEYCTQLPI
                     HPRHPYVGDLVFTAFSGTHQDAIKKGFSAKGTEARWDLPYLPIDPADVGRSYDSVIRI
                     NSQSGKGGIAYLLETEYGVVMPRRLQVEFSAEVQRHTDQHGGEMEAAKIWALFSQTYL
                     ETVNPVRYVKHHLYEEGTAQGIRLTLQMNGETHELTGVGNGPIDAAVHALRKLGIALQ
                     VRSYEERSMGSSREGGDAKACAFMEVTQTGGDRECYGVGLDANIVTASIKALMSGVNR
                     LATGGKNAKAA"
     gene            complement(1014..1976)
                     /gene="gcvA"
                     /locus_tag="PLD53_10600"
     CDS             complement(1014..1976)
                     /gene="gcvA"
                     /locus_tag="PLD53_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HRC61409.1"
                     /translation="MSYRLPPLNSLRAFEAAARHLSFKKASEELAVTPTAISHQIRGL
                     EDYLGFALFHRLTRALDLTNEGRAMLPKVREGLECFAESIESTRPREAGGRLLLSTPP
                     AFASRWLIPHLPKFTARHPEIELHLKSSLKTIDPPDATVVSGFEAIDVRDDESVLSVR
                     FGRGRYAGCRVERLFSPAYTAVCSPRLLTGMRPLNVPADLRYHDLLHDDTIPEQMDRP
                     SWSEWLQLAGVEGLNDHDGTHFSDSSLALTAVIDGLGIALASKPLVSAEVAAGRLVIP
                     FDIVVRRPQAYFLVTPEAVAERPVIHAFSQWLLEEAAKVQEDRP"
     gene            2089..2259
                     /locus_tag="PLD53_10605"
     CDS             2089..2259
                     /locus_tag="PLD53_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61410.1"
                     /translation="MESNINIHRIIEKARRERSIALGNLIASGTRKSVSWLLNRGNRL
                     LQVMLMSPVNSR"
     gene            2590..>3489
                     /locus_tag="PLD53_10610"
     CDS             2590..>3489
                     /locus_tag="PLD53_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRC61411.1"
                     /translation="MTSPFSPEATTEPPQRVVKIRRDYNTWVANETLEDYALRFTPRS
                     FRKWSEMRVANTAFGASSFLVLEAVGATMLVNGGFINAFWAILATGLIIFLIGLPISH
                     AAARYGLDMDLLTRGAGFGYIGSTVTSLIYASFTFIFFALEAAIMAYALELAFHIPPA
                     WGYLICALVVIPLVTHGVTAISRLQAWTQPLWLALLILPYAFLLWQEPEVLSELTGYA
                     GEKGLGGAFSMPLFGAALTVGIALVTQMGEQADYLRFMPERTAKNRNRWWLALVLGGP
                     GWVLPGVAKMLGGALLAYLALRNA"
BASE COUNT          753 a         1033 c          992 g          711 t
ORIGIN      
        1 gagcgcaccg gcaacgtcga cctcgtcacg ctggcgctca atctctatac ccagggtatt
       61 gcgccgaatc tcgatttttc ggatatcaac gccgttgccc gcaacttcga gtattgcacg
      121 caattgccga tccatccgcg tcatccctat gtcggcgatc ttgtattcac cgcgttctcc
      181 ggaacgcacc aggatgcgat caagaaggga ttttcagcga agggaactga ggcgcgttgg
      241 gatttgcctt atctgccgat cgacccggcc gacgttggac gcagctacga ttcggtaatc
      301 cgcatcaata gccagtcggg caagggaggc atcgcctatc tgctcgaaac ggaatacggg
      361 gttgtcatgc cgcgccgctt gcaggtcgag ttttcggccg aggttcagcg ccacaccgac
      421 cagcatggtg gcgagatgga agcagccaaa atctgggcgc tgttttcgca gacttacctt
      481 gaaaccgtta accccgttcg ttatgtcaag catcatctct atgaagaagg cacggcgcag
      541 ggaatacgcc tcacgctgca gatgaatggt gaaacccatg aactgacggg tgtaggcaac
      601 ggcccgattg atgccgccgt ccatgccttg cgcaaactcg gtattgcgtt gcaggtgcgt
      661 agttatgaag agcgctcgat gggcagcagc cgcgaagggg gcgatgccaa agcgtgtgct
      721 ttcatggaag tcacgcaaac cggaggcgac cgcgagtgtt acggtgtcgg cctggacgcc
      781 aacatcgtca cggcttcgat caaggcgctg atgagcgggg tcaaccgcct cgctaccgga
      841 gggaagaacg cgaaggctgc ctgagcatcg tcagccgagc gtcccggccg cagcgcgatt
      901 gccctgatga ctgcgttcca ggattacgtc ccggaacgca gtctttgaag cagcttgcct
      961 ttttgcaaga cggcaaacag tacctgttct cggtgtagta ttaccgacga aactcatggc
     1021 cgatcttctt gcaccttggc cgcttcttcc agcagccatt ggctgaaagc atgaatgacg
     1081 ggacgctctg caacggcttc gggcgtaacc agaaagtacg cctgaggtcg acggacaaca
     1141 atatcgaacg ggatcaccaa gcggcccgcg gccacctcgg cactaaccaa cggcttggag
     1201 gccaatgcga tgccaagacc atcgataact gcggtcaatg cgaggctgga gtcactgaaa
     1261 tgtgttccgt catggtcatt gaggccttca acacccgcga gctggagcca ctcgctccag
     1321 gacggtcggt ccatctgttc cggaatggtg tcgtcatgca gaagatcgtg atagcgcagg
     1381 tcggcgggta cgttcagcgg acgcattccc gtcagcagtc gaggactgca tactgccgtg
     1441 taggccggcg aaaacaatcg ctcaacccgg cagccggcat aacggccgcg accgaagcga
     1501 acggacagca ctgattcgtc atcgcgcacg tcaatcgcct cgaaaccgga aacgactgtt
     1561 gcgtccggag gatcaatggt tttcagagaa gatttcaaat gcaattcaat ttccgggtgg
     1621 cgtgcagtga acttgggcag atgaggaatc aaccagcgcg aggcaaaggc gggaggcgtt
     1681 gaaagcagaa gacgcccacc ggcctcgcga ggtcgtgtgc tctcgatgga ctccgcaaag
     1741 cattcaagac cttcgcgtac cttcggcaac attgcccggc cttcatttgt cagatccaga
     1801 gctcgcgtca gtcgatgaaa cagagcaaag cccagatagt cctcaagacc acgaatctga
     1861 tggctgatcg cggtcggcgt cacggcaagc tcttcgctgg ccttcttgaa actgaggtga
     1921 cgcgcggccg cttcaaaggc gcgcagtgag ttcagcgggg ggagtctata actcatacgg
     1981 ctgatatttt ctcatgcatc ggctgacaaa tgatcgtttg ttgcgacgca acattcttct
     2041 tacactgcat gcctacccga accacgggga aatgcaagga gtagcaacat ggaaagcaac
     2101 atcaacatcc acaggatcat cgaaaaggcg cgtagagaac gtagcatcgc attgggtaac
     2161 ctgatcgcct caggcacccg caaatcagtt tcatggttac tcaatcgggg taacagatta
     2221 ctgcaagtca tgctgatgtc acctgtaaat tcgcgctgag catccatccg cgttcaaatc
     2281 cgcaacctct caattccatc ccggccgctc gccaatgagc aaaacctgtt tgccggagtg
     2341 acggttacaa catctggccg tagcttacgc cgaattccat acccatgaac tgatgccatc
     2401 cacccattgg gcaggacggc ttgtcgaaca agcgcgggca atcgagaagc acgctttgtt
     2461 tcgatgacac tcgccacaac agttgtggca cgagaagcca cctgccaccg cctgacgcac
     2521 tagaccacgg aacctccagc acgacacccg gaggttacaa tcggggctct ttttcagtgc
     2581 ctgcccgtta tgaccagccc gttctcgcca gaagccacca cggagccccc gcagcgtgtc
     2641 gtaaagattc ggcgcgacta caacacctgg gtggccaacg aaacgctcga agattacgcc
     2701 ttgcgtttca caccgcgcag ttttcgcaag tggtcggaaa tgcgggtggc caacaccgct
     2761 tttggcgcct catcctttct ggtgctcgaa gccgtgggcg cgaccatgct ggtcaatggt
     2821 ggtttcataa acgccttctg ggccattctg gcaaccggcc tgatcatttt cctgatcggg
     2881 ttgccgatca gtcacgccgc tgctcgctac gggctggaca tggacctgct gacacgtggc
     2941 gcgggctttg gctatatcgg ctcgaccgtc acgtcgctga tctatgcgtc attcaccttc
     3001 atcttcttcg cgctggaagc ggcgatcatg gcctacgcgc tggaactcgc cttccatatt
     3061 cccccggcgt ggggttacct gatctgcgca ctggtggtca ttcccctcgt cacgcacggg
     3121 gtcaccgcca tcagccgcct gcaagcgtgg acgcagccgc tgtggctggc cttgctgatt
     3181 cttccgtacg ctttcctgct gtggcaggaa cccgaagtcc tgagcgagct gacaggctac
     3241 gcaggggaaa agggactggg cggcgcattc agcatgccgc tgtttggcgc agcacttacc
     3301 gtcggtatcg cactcgtcac gcaaatgggc gaacaggcgg actacctgcg cttcatgccg
     3361 gagcgcaccg caaagaaccg caaccgctgg tggctggccc tggtgctcgg gggccccggc
     3421 tgggttcttc ctggcgtggc aaaaatgctc ggcggcgccc tgctcgccta tctcgccttg
     3481 cgcaacgcc
//
LOCUS       DAORPM010000253         3482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_8927_length_3482_cov_8.953604, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000253 DAORPM010000000
VERSION     DAORPM010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8927_length_3482_cov_8.953604"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1120)
                     /gene="hemC"
                     /locus_tag="PLD53_10615"
     CDS             complement(143..1120)
                     /gene="hemC"
                     /locus_tag="PLD53_10615"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRC61412.1"
                     /translation="MSNSPDSPDSPNSPNPTPSRIVIASRESRLALWQAEHIQSRLSE
                     LYPRCRVEILGMTTRGDQILDRPLSQIGGKGLFIKELEVAMQEGRADLAVHSLKDVPM
                     EMPEGFSLTAIIRRENPCDAFVSNRYDGIDALPAGAVVGTSSLRREAILRASYPNLRI
                     EPLRGNLDTRLRKLDEGQFDAIILAAAGLIRLGLKERIKAVLSPEQSLPAPGQGALGI
                     EVLSDRADAIAAVAPLDDAETAHCVRAERAFSRALGGSCQVPLGGYAVLENGVLRLRG
                     FVGTPDGKQMVRGELSGEPADDESIGQGLAQQLRDRGANAILASLTTNR"
     gene            complement(1280..1747)
                     /locus_tag="PLD53_10620"
     CDS             complement(1280..1747)
                     /locus_tag="PLD53_10620"
                     /inference="COORDINATES: protein motif:HMM:NF020254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HRC61413.1"
                     /translation="MSTPASIDVPEFDRRLRRRLELICESFLNLTGQPLLEDSTPERL
                     WSAPRVIVAHGIEAEPVFFYGNRMALELFEMDFASFTHLPSRYSAEPLERQERARLLE
                     RVRRDGFIDDYAGVRISATGKRFRIEQAVVWNLIDGNGDIHGQAATFDRWKPI"
     gene            complement(1744..2523)
                     /locus_tag="PLD53_10625"
     CDS             complement(1744..2523)
                     /locus_tag="PLD53_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRC61414.1"
                     /translation="MNSVPTLSVMIVDDEAPARTRLRDLLADVAAEVPNAVVAEAANG
                     LLAVEATEGCSVDVALVDIRMPKMDGIELARHLTRLEHPPAIIFVTAYDSYAVQAFEL
                     NAIDYLVKPVRAQRLVDALQKVRQNRTVAPQVLSQIQQGTRTHLSCHERGRLLLIPLP
                     EILYFKADLKYVTARTASREYLLDESLTHLEEEFSARFIRLHRSALVARDAIAGFEKN
                     ATEDAETQWQALLRGVPDRLPVSRRQWPLVKSYAKQLSSGV"
     gene            complement(2520..3482)
                     /locus_tag="PLD53_10630"
     CDS             complement(2520..3482)
                     /locus_tag="PLD53_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRC61415.1"
                     /translation="MLLGVNLLAFAAALVQGTDIGDCIQRYVDMAAWVQPLLLLNILL
                     LALLANVLRRVRPWIGQVVVILIAAASSAQLFGVWSMIAIDGGGWQRLLRAALLGGLV
                     AATMLSYFVLRARSFSPALAEARLQALTARIRPHFLFNSLNAVLSLIRAEPERAETAL
                     EDLADLYRALMRDHRDLLPLSDEIALCRQYLDLEKLRLGERLNVEWEMADVPVDLNVP
                     PLMLQPLLENAVYHGIEPSGENGTVRIGMARKGNELHIDLANPCGRGDSSKGGNHMAL
                     ANIRERLALYYDLEARLETNEVILPDGLREYRVHIALPCRSDKP"
BASE COUNT          746 a         1083 c         1012 g          641 t
ORIGIN      
        1 ggcaatgtat cgtccgccgg cccgatctcg agcagaggga agcgtatcgg gtcgccgccc
       61 agatccccga tcatttccgc gagcttgccg gcctgggccg ctgggcgcgt caccagaata
      121 cgcttgccct gcagcgtttt cttcatcggt tggtagtcag tgaagccagt atcgcgttcg
      181 cgccccgatc acgcagttgt tgggccaaac cttgcccaat ggattcgtcg tctgccggct
      241 ctccactcaa ttcgccccgg accatctgct tgccgtccgg agtgccgaca aaaccgcgaa
      301 gccggagtac accgttttcc agaaccgcgt agccgccaag cggcacctga caactgcccc
      361 cgagcgcacg ggagaaggcc cgctcggcac gcacacagtg cgcggtttcc gcatcatcga
      421 gcggcgccac tgccgcaatg gcatccgcac ggtcggacag cacctcgata cccagcgccc
      481 cctgccccgg cgcgggcagc gactgttcgg gcgacagaac ggccttgatg cgctctttga
      541 ggccgaggcg gatcagaccc gctgcagcga gaataatcgc gtcgaactgc ccttcgtcaa
      601 gtttgcgcaa gcgggtgtca aggttgccgc gcaggggctc gatcctgaga ttcgggtaac
      661 tggcgcgtag aatggcctcg cgccgcaggc tggaggtgcc caccacggca cccgccggca
      721 acgcgtcgat tccgtcgtac ctgttcgaaa cgaaagcatc gcacgggttc tcgcggcgga
      781 tgatggccgt cagcgaaaac ccttcaggca tctccatcgg aacatccttg agcgaatgca
      841 ccgccaggtc ggcgcggcct tcttgcatgg ccacttcgag ctccttgatg aacaggccct
      901 tgcctccgat ctgtgagagc ggacgatcaa ggatctggtc gccacgggtg gtcatgccga
      961 gaatctcgac acggcatcgc ggatacagct cactcagacg cgactggata tgttcggcct
     1021 gccacagggc aagacgtgat tcacgcgagg caatgacgat acgcgacgga gtcgggttcg
     1081 gggagttcgg ggaatccggg gaatccggag aattagagat gttcaagacg ttttccaggc
     1141 catgtggcag ccaaaagggt cagcacttgc gatcatgtgg cacgaggcca cgcagctcca
     1201 aggggagcga ttctaacatg tctgatcacc ttgcacgccc ggcgaccagc gccatggacc
     1261 gacctcgaag gccaacccgt caaatcggct tccagcgatc aaaagtcgct gcctgcccat
     1321 gaatatcgcc gtttccgtcg atcaggttcc agacgacggc ctgttcgata cgaaagcgtt
     1381 tgcccgtcgc tgaaatcctt acgccggcgt agtcgtcgat gaaaccatca cggcggacgc
     1441 gctcaagcag gcgcgcacgc tcctggcgtt ccaacggttc ggcggagtag cgtgagggta
     1501 agtgggtaaa gctcgcaaag tccatttcga agagctcaag cgccatccgg ttgccataga
     1561 agaaaaccgg ttcagcctcg atgccgtgcg cgacgatcac acgcggagca ctccacaaac
     1621 gctccggagt ggagtcttca agcaacggct ggccggtaag attcaggaag ctttcgcaaa
     1681 tgagttccag cctgcgccgt agccgcctgt cgaattccgg cacgtcaata cttgctggcg
     1741 tgctcatacc ccggaactga gctgcttcgc gtaggacttg accagtggcc actggcggcg
     1801 actgaccggc agtctgtcag gaaccccacg caacagggcc tgccattgtg tttccgcgtc
     1861 ttctgtagca ttcttttcga agccggctat cgcatctctg gccaccaacg cactgcggtg
     1921 aagccgaatg aaacgcgcgg aaaactcttc ctcaagatgg gtcagcgact cgtcgagcag
     1981 gtattcccga ctggcggttc gcgccgtgac atatttgaga tctgccttga agtacaggat
     2041 ttcgggcagc ggaatcagca gcaaacggcc tcgctcgtgg catgaaaggt gcgtccgcgt
     2101 tccctgctgg atttgcgaca gcacctgcgg cgcaaccgtc ctgttctgtc gaaccttctg
     2161 cagagcgtcg accagccgct gcgcgcgaac cggcttgacc agataatcga tggcgttgag
     2221 ctcgaaagcc tgcaccgcgt aactatcgta ggccgtcacg aaaatgatgg ccggtggatg
     2281 ttcgagccga gtcaagtgcc gcgccaattc aatgccgtcc atcttcggca tgcgaatatc
     2341 gaccagggcg acgtccaccg aacacccctc tgtcgcctca acagccagta atccgttggc
     2401 cgcctcggcg accaccgcat tcggcacttc agccgccacg tcggcgagca aatcccgcaa
     2461 gcgcgtccgc gccggagctt cgtcgtcgac gatcatcacg gacagggtcg gcacggaatt
     2521 catggtttat cactccggca aggtaaagcg atatgcaccc ggtactcgcg aagaccgtcg
     2581 ggtagtatca cttcgttcgt ttcaagacgg gcctcgagat cgtagtaaag cgccagacgc
     2641 tcgcggatat tggccaaggc catgtggttt cccccttttg atgagtcgcc tcggccgcag
     2701 ggattggcca ggtcaatgtg cagttcgtta cccttgcgcg ccatgccaat gcggaccgtt
     2761 ccattctcac ctgatggttc gataccatgg tagaccgcat tctccagaag gggctgcaac
     2821 atcagcggcg gcacattcag atcgacgggg acgtcggcca tctcccactc gacattgagt
     2881 cgctcaccaa gtcgcaattt ttcgagatca aggtactggc gacacaacgc aatctcgtcg
     2941 gacaagggca acaggtcgcg gtgatcgcgc atcagcgcgc gataaaggtc ggccaaatcc
     3001 tcaagagcgg tttcggcgcg ttcgggttcg gccctgatca gggatagaac ggcgttcaga
     3061 ctgttgaaca aaaaatgcgg acggatacgt gcggtcagcg cttgcagccg ggcctcggcc
     3121 agcgctggag aaaatgatct ggcacgcagt acaaagtacg agagcatcgt cgcggcgacc
     3181 agcccaccca gcagtgcggc ccgcaacagc cgttgccagc caccaccatc gatcgcaatc
     3241 atgctccaga caccaaacaa ctgtgcagag gaagcagcgg caatgagaat cacgactacc
     3301 tgcccgatcc acggcctcac acgccgaagc acattggcaa gtagcgccag cagcaagata
     3361 ttcaaaagca gcaagggttg cacccaggcc gccatatcga catagcgttg gatacaatcg
     3421 ccaatgtctg tgccttgaac cagcgccgcg gcaaaggcga gcagattcac gccgagcaga
     3481 at
//
LOCUS       DAORPM010000254         3460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9035_length_3460_cov_14.153891, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000254 DAORPM010000000
VERSION     DAORPM010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9035_length_3460_cov_14.153891"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1992
                     /locus_tag="PLD53_10635"
     CDS             <1..1992
                     /locus_tag="PLD53_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRC61416.1"
                     /translation="VDRLIEIQNGVEVSPLKVIGETEKRGTEVHFMADEEIFGQVEFH
                     YEIIAKRLRELSFLNNGVKIRLLDQRTGKEEDFAFLGGVKGFVEYINRTKTVLHPNVF
                     SSIGESTTPNGVIGVEVAMQWNDTYAEQVLCFTNNIPQSDGGTHMTGLRAAMTRVLNK
                     YIDEHDIAKKAKVDITSDDMREGLVCVLSVKMPDPKFASQTKMKLVSSEARPAVEEVV
                     AARLTEFLQERPLDAKVITGKIVEAARARDAARKAREMTRRKGLLDGVGLPGKLADCQ
                     EKDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKLISSQEIVT
                     LITALGTGIGKDEYKPEKLRYHRLIIMTDADVDGAHIRTLLLTFFYRQMPELVEGGHI
                     YIAQPPLYKVKHGKSERYIKDDQALNHFLLALALEDATLTPKAGALEISGPALEELAR
                     EYLLAEAVINRLSHMINPQVLHALIEANLKMDLDDEVSAKASAEALMNVIGRKIGVHI
                     FARYDDVKERWQLRLEKILHGNLKVGVLDEDLLVSGDYVQLRKTAEIMAGLFGAGGFV
                     ARGDKKQPVSSFAETMSWLLSEVERGISKQRYKGLGEMNPEQLWETTMDPKVRRLLKV
                     QIEDAIGADEIFTTLMGELVEPRRNFIESNAQAARNIDV"
     gene            complement(1997..2923)
                     /gene="fmt"
                     /locus_tag="PLD53_10640"
     CDS             complement(1997..2923)
                     /gene="fmt"
                     /locus_tag="PLD53_10640"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRC61417.1"
                     /translation="MKLVFAGTPVFAATVLRDIIASGHHVLSVLTQPDRPTGRGMALH
                     SSPVKALALASGIEVFQPPTLRDSAAQERVRVLGAEVMVVAAYGLILPQAVLEIPRFG
                     CINVHASLLPRWRGAAPIQRAILAGDIETGVSIMQMEAGLDTGPVLLSERLSITEDDT
                     AGTLHDKLAVLGGRLIVEALGKLPLPLSLQPETGVTYASKIEKAEAPLDWRLPATQLA
                     RQVRAFNPYPGATVGLDGNTVKVWRAEAQAETGGSPGAVIAADKRGILVTCGEGTLRL
                     TELQKAGGKRLPVSAFLTGTPILPGATFDNLS"
     gene            complement(2920..3423)
                     /gene="def"
                     /locus_tag="PLD53_10645"
     CDS             complement(2920..3423)
                     /gene="def"
                     /locus_tag="PLD53_10645"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRC61418.1"
                     /translation="MPLLPILRYPDPRLKTVAAKVENIDDSIRRLARDMAETMYEAPG
                     IGLAATQVNVHMRVIVIDASEARDQLLVLINPELVESEGLQVCEEGCLSVPGIFDKVE
                     RAESVVVRYLDTDGEDQTVAADGLLAVCLQHEIDHLQGRVFVEHLSLLKQTRIKAKLA
                     KQSRITA"
BASE COUNT          838 a          897 c          984 g          741 t
ORIGIN      
        1 gttgaccgct tgatagagat tcagaatggc gtcgaagtgt caccgctcaa ggttattggc
       61 gagacggaaa agcgtggaac agaagtgcac ttcatggccg acgaagaaat ctttggccaa
      121 gtcgagttcc actacgagat tatcgccaaa cgtctgcgtg agctttcttt cctgaataat
      181 ggagtgaaaa ttcgcctgct tgatcaacgt accggaaagg aagaggattt tgcctttttg
      241 ggtggtgtga aaggcttcgt cgagtacatc aatcggacga aaacggttct gcaccctaat
      301 gttttcagtt caataggtga atcgaccacc ccgaacggcg tgattggtgt cgaggttgcg
      361 atgcagtgga acgacaccta tgctgagcag gtgctgtgtt tcaccaacaa catcccgcag
      421 tcggatggcg gaactcacat gaccggcttg cgcgcagcaa tgacgcgtgt actgaataag
      481 tacattgatg agcacgatat cgcaaaaaag gccaaagtcg acatcaccag cgatgatatg
      541 cgcgaggggc tcgtttgtgt gctctcggta aaaatgccgg atccgaagtt tgcttcacag
      601 accaagatga agctggtttc ttctgaagcg cgtccggcag ttgaagaagt cgtcgcggcc
      661 aggctgaccg aattcttgca ggagcggccg cttgacgcga aggtcattac cggcaaaatt
      721 gttgaggcag cacgggcacg tgacgccgct cgcaaagcac gtgagatgac acgccgaaaa
      781 gggctgctgg atggggttgg gctgccggga aaactggccg actgccagga gaaggatcca
      841 gcgctgtgcg agctttacct agttgagggt gattcggccg ggggctctgc aaaacagggt
      901 cgcgaccgaa agttccaggc gatattgccg ctcaagggaa agatactcaa cgttgagaag
      961 gcgcgtttcg acaaattgat ttcctcacag gaaatcgtga ccttgattac cgcgctcggt
     1021 acgggtatcg gaaaggacga atacaaaccg gagaaacttc gttatcaccg actgatcata
     1081 atgacggatg ctgacgttga cggcgcccac attcgcacct tgctcctaac cttcttttac
     1141 aggcaaatgc cagagttggt cgaaggcggt catatctata ttgcacagcc cccgctgtac
     1201 aaggtcaaac acggaaaatc agaacgctac atcaaggatg atcaggcact caaccatttc
     1261 ctattggcgc tcgcgcttga agatgccacg ctgacgccta aagctggagc attggaaatt
     1321 tctggcccag cgctcgaaga gttggctcgt gagtatcttc ttgcagaggc ggtcatcaac
     1381 cgcctatcac acatgatcaa cccacaggtt ctgcacgccc tgatcgaagc caatctgaag
     1441 atggatttgg acgatgaagt gtccgccaag gccagtgctg aagctctgat gaacgttatc
     1501 ggcaggaaga tcggtgttca tatttttgcg cgctatgacg atgtaaagga gcgctggcag
     1561 ttgcgcctcg agaaaatact ccacggcaac ctgaaagtcg gcgtgctcga cgaagacctg
     1621 ttggtcagcg gagactacgt gcagctacgc aagacagcgg agatcatggc gggattgttt
     1681 ggtgccggtg gttttgtcgc ccgtggtgac aagaagcagc cggtgtcaag ctttgccgaa
     1741 accatgagct ggctactcag cgaggtcgag cgtggcatca gcaagcagcg ctacaaaggt
     1801 ttgggtgaga tgaatcccga gcagctctgg gaaacaacga tggatcccaa ggttcggcgt
     1861 ctgcttaagg ttcagatcga agacgcgatc ggtgccgatg aaatattcac aaccttgatg
     1921 ggcgagttgg tcgaaccaag gcgaaatttc atagagtcga atgcccaggc agctcgtaat
     1981 atcgatgtct gagcagtcag gacaggttgt cgaaggttgc tccaggaagt atgggggttc
     2041 cagtcaggaa cgcgctaacg ggcagtcgtt tgccaccagc cttttgcaac tctgtcaatc
     2101 gtaacgtacc ctcgccacaa gtaaccaaga taccgcgttt gtcggccgca ataaccgcgc
     2161 ctggcgatcc accggtttcg gcttgggcct cggctcgcca aaccttgact gtgttgccat
     2221 ccagaccgac agtcgcgccg ggatatgggt tgaacgccct gacctgcctg gccagctgcg
     2281 tggctggcaa gcgccaatca agtggtgctt cagctttctc gatcttggaa gcgtaggtca
     2341 ccccggtttc tggctgcaag ctcagcggca agggcaattt accgagtgcc tcgacgataa
     2401 gacgacctcc gaggacggct agcttgtcgt gcaaggttcc ggccgtgtca tcctcggtaa
     2461 tcgatagtct ttccgaaagc aacactggac cggtatccag acctgcttcc atctgcatga
     2521 tgctgacgcc ggtttcgatg tcaccggcca gaatcgcccg ctggatcggt gcagcaccac
     2581 gccagcgcgg cagcagtgac gcgtggacat tgatgcagcc gaaacgcggt atttcgagta
     2641 ccgcctgcgg caagattagt ccatacgcgg cgacaaccat gacctccgcg ccgaggactc
     2701 gcacacgttc ctgtgccgcc gaatcacgca gtgtaggtgg ttggaaaacc tcaatgcctg
     2761 aggccagcgc tagggcctta acgggcgacg aatgaagtgc cataccgcgt cctgtcggcc
     2821 gatccggctg tgtaagcact gacaacacgt gatgtccgga tgcaataatg tcgcgcagga
     2881 cggtggcggc aaacaccggc gtaccggcaa agaccagctt catgcggtaa tgcgcgactg
     2941 cttggctaat ttggccttga tgcgtgtctg cttgagaagc gagagatgtt ccacaaaaac
     3001 tcgaccctga aggtgatcta tttcatgctg caaacagact gcaagcaaac cgtcggcagc
     3061 aacggtttga tcttcaccgt ccgtatcaag atagcgaacg acgacgcttt ccgcacgttc
     3121 gaccttgtcg aagataccgg gcaccgagag acatccttcc tcacaaacct gaagcccttc
     3181 actttcgaca agttccggat tgatgagcac cagcaactga tccctggctt cagaggcatc
     3241 gatgacgata acccgcatat gtacgttcac ttgcgtcgcg gccagaccga ttcccggtgc
     3301 ctcgtacatc gtttcggcca tatcacgagc gagacgtcga atgctatcgt caatattttc
     3361 aaccttggcg gcgacggttt tcaagcgcgg gtcagggtaa cggagtattg gcagtagtgg
     3421 cataaaaagc ttgatcgaag aagtaattac gtgcagaatc
//
LOCUS       DAORPM010000255         3445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9098_length_3445_cov_23.682596, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000255 DAORPM010000000
VERSION     DAORPM010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9098_length_3445_cov_23.682596"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLD53_10650"
     CDS             <1..627
                     /locus_tag="PLD53_10650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRC61419.1"
                     /translation="DLMSGGLHRLWKAFTIARSGVREGARVLDVAGGTGDLALAFARK
                     VGKTGEIWLTDINNAMLTNGRDRLCDKGFLIPVAQCNAEKLPFPGDYFDCVTVAFGLR
                     NMTHKSTALAEMLRVLRPGGRLLVLEFSQVWKPLTPFYDFYSFKVIPRIGEIITKDEA
                     SYRYLAESIRVHPDQESLKALMEQAGFENVDYFNMAMGVVALHRGFKF"
     gene            639..1502
                     /locus_tag="PLD53_10655"
     CDS             639..1502
                     /locus_tag="PLD53_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HRC61420.1"
                     /translation="MKKALVAVFAVILTLGIFAEEAEAARFGGGRSFGMKRQAVTPKP
                     ASPAQAAPAPTNPASAAGAATTPKRNWMGPIAGLAAGLGIAALLSHFGMGEGMANIVM
                     IALLAMAAVFVFKLLFRRRSPVAHAATYPGEPMQFAGAGVTTTPPLSQLGTSSSSVSP
                     ATQHIPDGFDTEGFLRVAKLNFIRMQAANDAKNLEDIREFVSPEVFAEIKMQMDERGS
                     APQQTDVATLNAELLEVCTESNRHVASVHFSGMISDAAGAAAVPFDEVWNLSKPIEGG
                     GWVVAGIQQLN"
     gene            1750..2121
                     /locus_tag="PLD53_10660"
     CDS             1750..2121
                     /locus_tag="PLD53_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61421.1"
                     /translation="MRALTDRSSLFTAAKISGSAELAESLGFVFRNLRWDVESDLSLL
                     VGDIAARRLVEGSKQLVKWHVQQTTNLALNVAEYFTEEKPAIARSQNISAFCGDVGDL
                     RDAMDRLENRVASLESRPLKV"
     gene            complement(2163..2585)
                     /locus_tag="PLD53_10665"
     CDS             complement(2163..2585)
                     /locus_tag="PLD53_10665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRC61422.1"
                     /translation="MPLRVHVDVVSAEELIFAGETDFAVFPGEAGELGIYPRHTPLLT
                     RIKPGTVRLKVPDREEYELVYVSGGVLEVQPTLITLLADTAIRAHDLDEAKSIEAKRR
                     AEEALENRSSDVNYATAEAELAQAVAQLQTLKRIRKKN"
     gene            complement(2654..>3445)
                     /gene="atpD"
                     /locus_tag="PLD53_10670"
     CDS             complement(2654..>3445)
                     /gene="atpD"
                     /locus_tag="PLD53_10670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRC61423.1"
                     /translation="NVLDKVAMVFGQMNEPPGNRLRVALTGLTMAERFRDDGRDILFF
                     VDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGRLQERITSTKVGSITSIQAVYV
                     PADDLTDPSPATTFLHLDSTVVLSRDIASLGIYPAVDPLDSTSRQLDPQVVGEEHYKV
                     ARAVQMTLQKYKELRDIIAILGMDELSPDDKLAVYRARKIQRFLSQPFHVAEVFTGSP
                     GKYVPLKETIKGFKMIVEGECDSIPEQAFYMVGGIEEALEKAKTL"
BASE COUNT          725 a          973 c          971 g          776 t
ORIGIN      
        1 gacctgatgt ccggtggtct gcaccgcctt tggaaggcgt tcactattgc ccgtagcggc
       61 gtacgcgaag gcgctcgtgt gctcgatgtt gccggtggca cgggtgatct cgcgcttgct
      121 tttgcccgca aggtcgggaa gaccggcgaa atctggttga ccgacatcaa caatgccatg
      181 ctgaccaatg gccgcgatcg cctctgtgac aaaggattcc tgatcccggt agcacagtgc
      241 aatgccgaaa agttgccctt cccgggagat tactttgatt gcgtcacagt agcgtttggc
      301 ctgcgcaaca tgacccacaa gtccaccgca ctcgccgaaa tgcttcgcgt cctgcgcccc
      361 ggaggacgct tgctggtact ggagttctca caggtctgga agccactgac gccgttctac
      421 gacttttatt cgttcaaggt gattccgcgt attggcgaga tcatcaccaa ggacgaggcg
      481 agttaccgct acctggctga atcgattcgt gttcatccgg atcaggaatc actgaaggca
      541 ttgatggaac aagcgggttt tgagaacgtt gactacttca atatggcgat gggcgttgtc
      601 gcactgcacc gcggatttaa attttaatgg agccgtatat gaaaaaggca ttagtagctg
      661 tttttgctgt gattctgacc cttggcatct ttgcggaaga ggcagaagcc gcccgctttg
      721 gtggcgggag atcctttggc atgaagcggc aagctgtcac accaaaaccc gcctctcctg
      781 cccaggcggc tccggcgcct acaaatccgg cttctgctgc aggagcggca acaacgccca
      841 aacgcaactg gatgggaccg attgccgggc tcgctgccgg cctcggtatt gcggcgctgc
      901 tttcgcactt tggcatgggt gaaggcatgg ccaacatcgt gatgatcgca ttgctggcga
      961 tggctgcggt tttcgtcttc aagttgttgt tccgccgcag atcgcccgta gcgcatgctg
     1021 cgacgtaccc tggtgaaccg atgcagtttg ctggcgcagg cgtcaccacg acgccgccat
     1081 tgtcgcagct tggcacgagc tcatcttccg tttcccctgc aacccagcac attcccgatg
     1141 gctttgatac tgaaggattc ctgcgtgtcg caaagctgaa cttcattcga atgcaggccg
     1201 caaatgacgc caagaacctt gaagatattc gcgaattcgt cagtcctgaa gtgtttgccg
     1261 aaataaagat gcaaatggat gaacgcggaa gcgcacccca gcaaaccgac gtggcgactc
     1321 tgaatgcgga gcttctcgag gtatgtaccg agtcaaatcg tcatgttgcc agcgtgcatt
     1381 tcagcggcat gatcagtgac gcggcggggg cagcagcagt tcccttcgat gaggtgtgga
     1441 acttgagcaa gccgatcgag ggtggtggct gggtcgtggc cggaattcag cagctcaact
     1501 gagttcatat acgtattcgt tgttagctat tcgatgctgg tcgacgcagc gctcggattc
     1561 ataaaccatc ttctcgctgg agaagcttgg gcgcgtgacc gcctcaaagt gtttgcaggg
     1621 aaaacggtgc gcctggagca aggcgcactc tgcctgtcac tggcaatctc ttccaacggg
     1681 cttttcttcg ttgctaccgg aaagcaagca acggaagacg tcagcatcac tcttccatcc
     1741 gatgcgccct tgcgcgcgct gaccgaccgt tcctcgcttt tcaccgcggc aaagatcagc
     1801 ggctcggctg aattggcaga gagcctcggc tttgtcttcc gaaatttacg ctgggacgtg
     1861 gaaagcgatc tttcgctgct tgtaggcgat atagcggcgc gtcgtcttgt cgaaggcagc
     1921 aagcagctgg tcaagtggca tgtgcaacaa accacaaacc tggcattgaa tgtggccgaa
     1981 tacttcacag aggaaaagcc ggctatcgct cgcagccaga acatttctgc cttctgcgga
     2041 gacgtcggtg atttgcggga tgcgatggat cgacttgaaa atcgggtggc atccctggaa
     2101 agccgaccgc tgaaggtctg accatcttcc tgctgattgc agtcgatcct gcgaatcgct
     2161 ggtcagttct tctttcgaat ccgcttgagt gtctgcaact gcgcgacagc ctgcgccaat
     2221 tcggcctctg cagtcgcata gttgacgtcg gaggagcggt tctccagggc ttcttcggct
     2281 cgtcgcttgg cttcgatgga cttggcctca tccaggtcgt gcgcacgaat cgccgtgtcg
     2341 gcgagcagtg taatcagtgt tggctgtact tccagcactc cgcccgatac gtacaccaac
     2401 tcatactctt cgcgatccgg tactttcaga cgtaccgtac ctggcttgat acgggtcaac
     2461 agcggcgtat gacgcgggta gatacccaat tcaccagcct cgccgggaaa caccgcaaaa
     2521 tcggtctctc cggcaaaaat cagttcctcg gcactgacga cgtcaacatg tactctaagt
     2581 ggcatggttt ccttccccta cgcagatcgc gtcgaatttt ttggaacgac acggctcccg
     2641 cgacggactg agcttaaaga gttttggctt tttccagggc ttcctcgata ccgccgacca
     2701 tatagaaggc ttgctcgggg atgctgtcgc actcaccttc aacaatcatc ttgaaaccct
     2761 tgatcgtctc cttgagcggc acatacttgc ctggcgaacc ggtaaagact tcggcgacat
     2821 gaaacggctg cgacaggaaa cgctggattt tgcgcgcgcg ataaacggcc agcttgtcat
     2881 ccggtgacag ttcatccatg cccagaatgg cgatgatgtc ccgcaattcc ttgtacttct
     2941 gcaacgtcat ctgtaccgca cgtgcgacct tgtagtgctc ctcgccaacg acctgtgggt
     3001 caagctggcg cgatgtcgaa tcgagcgggt cgaccgccgg atagataccc agcgaagcga
     3061 tgtcacgaga caacacgacg gtcgaatcaa ggtgcaggaa ggtagttgcc ggcgacggat
     3121 cggtcaagtc atcggcgggc acatacacgg cctgaatcga ggtgatcgag ccgaccttgg
     3181 tcgacgtgat ccgctcctga aggcgcccca tttcttcggc cagggtcggc tgatagccca
     3241 cggcggacgg catccgccca agcagcgcgg acacttccgt tccggccaag gtataacgat
     3301 aaatgttatc gacgaagaac agaatgtcgc ggccatcgtc acggaaacgt tctgccatcg
     3361 tcaagccggt cagcgccaca cgcagacgat tgcccggcgg ctcgttcatc tgaccgaaca
     3421 ccatcgcgac cttgtcgaga acgtt
//
LOCUS       DAORPM010000256         3435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9142_length_3435_cov_11.036391, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000256 DAORPM010000000
VERSION     DAORPM010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9142_length_3435_cov_11.036391"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /locus_tag="PLD53_10675"
     CDS             complement(<1..2038)
                     /locus_tag="PLD53_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC61424.1"
                     /translation="MTNRSSPPPDTGNTFPSQSLSALPPEFGAQLETLLAADETILAW
                     LETDLDTRLHFAPGLVLLTNHRLLARSGDDDNWQSYPLRSGLTLVRNDHSGVGSLELL
                     DADSRQSIWRYSLGGDIAAGRLIEHFSRQLAFHLTGKAPSLPTQALCPKCETPLLAGQ
                     EECPLCSKEIHAPPSTWTLLRLWRFAYPYRWQLLAGFLLSLGSTAATLVPPYLTMPMM
                     DKVLIPYQNGTPIDTALVTLYLSGLFGAALLAWVLGWARTYILALVSERIGADLRTTT
                     YEHLLKLSQEYFGGKRTGDLMARIGSETDRINIFISLHFLDFVTDVLMITMTTFILVS
                     INPWLALVTLLPLPIIAWLIHVVRERLRTGFERVDRIWAEVTNVLADTIPGIRVVKAF
                     AQESREAARFRAANAHNLEVNDKVNKIWSLFSPTVTLLTEIGLLIVWAFGIWQISKDE
                     ITVGVLTAFLAYISRFYLRLDSMSRIVSVTQKAAAGAKRIFDILDHVSSVPEPVNPVH
                     LTNVTGRIELRNVGFRYGTRSVTRDISMTVEPGEMIGLVGHSGSGKSTMVNLICRFYD
                     VSEGAILIDGVDIRAVPVAEYRQHIGLVLQEPFLFFGTIAENIAYGKPGATREEIVTA
                     ARAAHAHEFILRLAHGYDSLVGERGQALSGGERQRISIARALLIDPKILILDEAT"
     gene            2229..>3435
                     /locus_tag="PLD53_10680"
     CDS             2229..>3435
                     /locus_tag="PLD53_10680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRC61425.1"
                     /translation="MLAHDALKVSSDGSEVAQDFNPLSSKAGQRALQLFVRAVESSAN
                     GIMITDAIDPKHPIIYVNPAFERITGYPASAVIGRNARFLAGSALEQPALEVIRNALR
                     QHRAAQAVVQCFRQDETLFWNQLSVTPVPDEHGEIGHFVSIFEDVSERVLRENRLLHL
                     STHDALTGLANRTLLADRLDQAVTHSVRDGGVVAVLLIDLDRFKQINDSLGHAVGDLL
                     LKTVAERLGHCVREGDTVARLGGDEFVLVLADLSRNEDASMLARKILAALSRPIDIDG
                     HELFVTPSIGISFYPQDGADASSLLRHADLAMYQVKSSGRNGFSCFSQDMNERAQEMA
                     ELESSLRQALEHDELTLYYQPKADLYSGEICGAEALIRWQHPVKGLVPPSQFIKLAEE
                     SGMILQLGEW"
BASE COUNT          675 a          975 c         1109 g          676 t
ORIGIN      
        1 aggtggcttc gtcaagaatc agaatcttcg gatcgatcaa caaggctctg gcaatggaga
       61 tgcgctggcg ctcaccgccg gagagggcct ggccgcgttc gcccaccagc gagtcgtagc
      121 cgtgcgccag tcgcaggatg aattcatgcg cgtgggcggc gcgggcggcc gtgacgattt
      181 cttcgcgcgt tgcgccgggt ttgccgtagg caatattttc ggcgatggtg ccgaaaaata
      241 ggaagggctc ctgcagtacc aggccgatgt gctgacggta ttcggccacc ggcaccgccc
      301 ggatatcgac accgtcgatc aggatggcgc cttcgctcac atcgtaaaag cggcagatca
      361 ggttgaccat cgtactcttg cccgatccgc tgtgccccac caggccgatc atctcgcccg
      421 gctcgaccgt catgctgatg tcgcgcgtga cgctgcgcgt accgtagcga aaaccgacgt
      481 tgcgcaactc gatgcggccg gtgacgttcg ttagatggac cggattgacg ggttccggaa
      541 cgcttgagac gtggtcgagg atatcgaaaa tccgtttggc gccggcggcg gccttttgcg
      601 tgacggaaac gatgcggctc atcgagtcga ggcgcaggta gaaccggctg atgtaggcga
      661 ggaaggcggt caatacgccg acggtgattt cgtctttcga gatctgccag atgccgaagg
      721 cccacacgat cagcagtccg atctcggtga gcagggtgac ggtcggcgaa aacagcgacc
      781 agatcttgtt gaccttgtcg ttgacctcga gattgtgcgc gttggctgcc ctgaaacgcg
      841 ccgcttcgcg actttcctgc gcgaaggctt tgaccacgcg gatgccgggg atggtgtccg
      901 ccagcacatt ggtgacttcg gcccagatgc ggtcgacgcg ctcgaagccg gtacgaagtc
      961 tttcgcgcac gacgtggatc agccaggcga tgatcggcag cggcagcagt gtgaccagcg
     1021 ccagccacgg attgatcgaa accaggatga aggtcgtcat ggtgatcatc agcacatcgg
     1081 tgacgaaatc gagaaaatgc agtgagatga agatgttgat gcggtcggtt tccgaaccga
     1141 tgcgggccat caggtcgccg gtacgcttgc cgccgaagta ctcttgcgag agcttcagca
     1201 ggtgctcgta ggtggtcgta cgcaggtcgg cgccgatacg ttcggagacc agcgccagaa
     1261 tataggttct cgcccagccg agcacccagg ccagcaatgc cgcgccgaac aggccggaga
     1321 ggtacagcgt gaccagcgcg gtgtcgatcg gggtgccgtt ctggtagggg atcagcacct
     1381 tgtccatcat cggcatggtc aggtagggcg gaaccagcgt cgccgctgtc gaccccaagg
     1441 agagcaggaa gccggccagc agttgccagc ggtatggata ggcaaagcgc cacagccgga
     1501 gcagtgtcca tgtcgagggc ggtgcatgga tttccttgct gcacagcggg cattcttcct
     1561 ggccggccag cagcggtgtt tcgcacttcg ggcaaagcgc ctgcgttggt aaggacggcg
     1621 ctttgcccgt cagatgaaag gccaactgcc gtgagaaatg ctcgatcagg cgcccggcgg
     1681 caatgtctcc gccgagcgaa tagcgccaga tcgactgccg tgagtcggca tcaaggagtt
     1741 ccaggctgcc gacgcccgag tggtcgttgc gtaccagcgt cagacccgaa cggagcggat
     1801 aagactgcca gttgtcgtcg tcaccggagc gggcgagcag tcgatgattg gtcagcagta
     1861 ccaggccggg ggcaaaatgc agtcgggtgt caaggtcagt ctccagccag gccagaatcg
     1921 tttcatccgc agcgagtagt gtctcaagct gtgcgccaaa ctccggaggc agggctgaca
     1981 aggattggct ggggaaggta ttgccggtat cggggggtgg agagctgcgg tttgtcattg
     2041 tcgagcgatt gttttgggca ggcggcgaga taagtgcctg aacgaccgag tataactgtc
     2101 ttcgccactt ttctggtgaa actccgccga cgtttcagat cggccgggaa gccttacatg
     2161 cgtcttggct cggtgttaat attccagcgc atcgtttggc cgatttgaag gagtcgaagg
     2221 cagtgattat gcttgcgcat gacgcattga aagtttcttc agacggcagc gaagtcgcac
     2281 aggacttcaa tccgctgagc agcaaggcag ggcagcgtgc gttgcagttg ttcgtgcgcg
     2341 ccgtcgagtc atcggccaac ggcatcatga ttacggacgc gatcgacccc aagcatccga
     2401 tcatctatgt caaccccgct ttcgagcgca tcaccggcta tcccgccagt gcggtgatcg
     2461 gccgcaatgc ccgttttctg gccggctcgg cactggagca gccggcgctt gaggtgatcc
     2521 gcaacgcgct tcgccagcac cgggcggcgc aggccgtggt gcaatgcttc cgccaggatg
     2581 aaacactctt ctggaatcag ctctccgtca cgcctgtgcc cgatgaacat ggggaaattg
     2641 gtcatttcgt cagcattttt gaggacgtca gcgaacgggt attgcgggaa aaccggttgt
     2701 tgcatctgtc cacccacgat gcgctgaccg gacttgccaa ccggacgctg cttgccgacc
     2761 ggctggacca ggcggtaacg cacagcgtgc gtgacggggg cgtcgtggcc gtcctgctga
     2821 tcgacctcga ccgcttcaaa cagatcaacg acagcctcgg tcatgccgtc ggcgaccttc
     2881 tcctcaaaac cgtggcggaa cggctcggac attgcgttcg tgaaggggat accgttgctc
     2941 gcctgggtgg cgacgagttc gtccttgtgc tcgctgacct ctcgcgcaat gaagatgcct
     3001 cgatgctggc ccggaagatt ctcgcggcat tgtctcggcc catcgatatc gacgggcacg
     3061 agctgttcgt cacgccgagc atcgggatca gcttttaccc tcaggatggc gcggatgcgt
     3121 cgtcgctgtt gcgacatgcc gacctcgcca tgtatcaggt caagtccagc ggacgcaacg
     3181 gtttcagctg cttctcgcaa gacatgaatg agcgtgcgca ggaaatggcc gagctcgaat
     3241 ccagtctgcg tcaggcactg gaacacgacg agttgaccct ttactatcag ccgaaggccg
     3301 acctgtattc cggtgaaatc tgtggcgccg aggcgctgat ccgctggcag cacccggtga
     3361 aggggctggt tccacccagc caattcatca agcttgccga agaatccgga atgatcctgc
     3421 aactcggcga gtggg
//
LOCUS       DAORPM010000257         3418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9231_length_3418_cov_6.840321, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000257 DAORPM010000000
VERSION     DAORPM010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9231_length_3418_cov_6.840321"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /gene="obgE"
                     /locus_tag="PLD53_10685"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             complement(<1..455)
                     /gene="obgE"
                     /locus_tag="PLD53_10685"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: protein motif:HMM:NF013205.2"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRC61426.1"
                     /translation="MKFVDEAYIDIVAGDGGAGCVSFRHEKYKEFGGPNGGDGGRGGH
                     VFAVADPSLNTLVDFRFSRRHEAKRGQHGMGSDMFGAAGDDITLRMPVGTIITDAETG
                     EVLYELLTPGEQIMIAKGGDGGFGNMRYKSAINRAPRQKTPGWPGDRKKL"
     gene            complement(541..801)
                     /gene="rpmA"
                     /locus_tag="PLD53_10690"
     CDS             complement(541..801)
                     /gene="rpmA"
                     /locus_tag="PLD53_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRC61427.1"
                     /translation="MAQKKGGGSTRNGRDSKPKMLGVKAFGGELISAGSIIVRQRGTR
                     FHPGTNVGLGKDHSLFALVDGHVSFGTKGALNKHTVSVTPAA"
     gene            complement(814..1125)
                     /gene="rplU"
                     /locus_tag="PLD53_10695"
     CDS             complement(814..1125)
                     /gene="rplU"
                     /locus_tag="PLD53_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRC61428.1"
                     /translation="MYAVIKTGGKQYRVASGEKIKVEQIAADVGQEIVIDQVLAVGNG
                     AELKVGTPLVSGATVKATVVAHGKHDKVRIFKMRRRKHYQKRQGHRQQFTELQIGAIA
                     A"
     gene            1328..2257
                     /locus_tag="PLD53_10700"
     CDS             1328..2257
                     /locus_tag="PLD53_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRC61429.1"
                     /translation="MLEVDKVIARRLDSGVPLVGEVARYIISAGGKRLRPVLLLLTCG
                     ALGYQGSQRFNLAAVVEFIHTATLLHDDVVDESTLRRGRSTANEAFGNPASVLVGDFL
                     YSRAFQMMVEADNMRVMQILAEATNIIAEGEVLQLMNMHDASLDEADYLRVIRSKTAK
                     LFEASARLGAILSASGPAFEEACAIYGQALGTAFQVVDDVLDYAGTAAEMGKNLGDDL
                     REGKATLPLIMAMQLGTTSQRDLIREAIETGAVTQLSLVVNIVRQTGALDATHDAAAQ
                     EAQRAIAAARTLPDNRFTEGLIQLASQLLERRH"
     gene            2282..2358
                     /locus_tag="PLD53_10705"
     tRNA            2282..2358
                     /locus_tag="PLD53_10705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2316..2318,aa:Pro,seq:cgg)
     gene            2450..>3418
                     /locus_tag="PLD53_10710"
     CDS             2450..>3418
                     /locus_tag="PLD53_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRC61430.1"
                     /translation="MSLIEKPSNFMAVAEPPSKDISAVALPGLARALVTAGKLGQKAA
                     EDIYRKALTGKTNFISELTGSGAVSASDLAHTMSTAFAVPLVDLDALDRNRLPKGLLD
                     PKTCQDFRVLVLSKRGNRLIVATADPADQAAAEKIKFATQMGVDWVIAEFDKLSRMVE
                     QSTTSVNEAMESFVGGEFEFDESSMDASAADAGEVDTSEVDDAPIVKFLHKMLLDAFN
                     MRASDLHFEPYEFNYRVRFRVDGELREIASPPIAIKDKLASRIKVISRLDISEKRVPQ
                     DGRMKLKVGPDRSVDLRVSTLPTLFGEKIVIRILDPSSAKLGIDALG"
BASE COUNT          697 a         1050 c          966 g          705 t
ORIGIN      
        1 agcttcttgc ggtcacccgg ccagcccggc gtcttctggc gcggcgcgcg gttgatcgca
       61 cttttgtagc gcatgttgcc aaaaccgccg tcgccgcctt tggcgatcat gatctgctcg
      121 ccgggcgtca gcaactcata gagcacttcg ccggtttcag cgtcggtgat gatggtgccc
      181 accggcatcc gcagcgtgat gtcatctccg gccgctccga acatgtcgga gcccatgcca
      241 tgctgcccgc gcttggcttc atggcgacgg gaaaagcgga aatcaaccaa ggtgttcagg
      301 ctgggatcgg ccacggcaaa tacatgtccg ccacgccccc catcgcctcc attggggccg
      361 ccgaactcct tgtatttttc atgcctgaac gaaacgcagc ctgcgccgcc atcgccagcg
      421 acgatgtcaa tataggcttc atcaacgaat ttcatcaggt atccagttta tacagaccga
      481 caacgcgcca aaacaacgaa gccccgacaa ggcggggctc cgtactgggc tgcgaaagaa
      541 tcaagcagcc ggggtgacgc tcaccgtgtg cttgttcagt gcacccttcg tgccaaagga
      601 cacgtggcca tccaccagcg caaagaggct gtgatccttg cccaggccca cattggtgcc
      661 gggatggaag cgggtaccgc gctggcgcac aatgatcgag cccgcgctga tcagttcgcc
      721 accgaaggct ttcacgccga gcatcttggg cttcgaatcg cgcccgtttc gcgttgagcc
      781 gccgcctttt ttctgtgcca tgacctgttc tccttaagcg gcaatcgcac cgatttgcag
      841 ttcggtgaac tgctggcgat ggccctgacg tttctggtaa tgcttgcgac ggcgcatctt
      901 gaagatgcga accttgtcgt gcttgccgtg cgccacaacc gtggctttca cagtcgcacc
      961 ggacaccagg ggcgtaccca ccttgagttc agcgccgttg ccgacagcca gaacctggtc
     1021 gatcacgatc tcctggccta cgtccgcagc aatctgttct actttaattt tttcgccgga
     1081 agcaacgcga tactgcttgc cgccggtttt tatgaccgcg tacatgtaaa cctcttgagt
     1141 gagttctgca gacggatttc cgcagagcca aggattgtag catggtttgc agccactaac
     1201 ctgctgtccg gcggtggccg aagtcccgcc cgggcggtcc ctataatccc gcaacttttc
     1261 ggtgaatccc accttggctg caacatccac ttccacgttc aacgctctgg cactcgtcgc
     1321 cgacgacatg ctcgaagtgg acaaggtcat cgcgcgccgg ctggattcgg gcgtcccgct
     1381 cgtcggtgaa gtggcccggt acatcatttc cgcgggcggc aaacggctca gaccggtgtt
     1441 gttgttgctg acctgtggcg ccctcggcta ccagggcagc cagcgtttca acctcgctgc
     1501 ggtcgtcgag ttcatccaca cggccacact gctccacgat gacgtggtgg acgaatccac
     1561 cctccggcgt gggcgctcga cagccaacga ggccttcggc aaccccgcga gtgtcctggt
     1621 cggcgatttt ttgtactccc gcgctttcca gatgatggtc gaagccgaca acatgcgggt
     1681 gatgcagatc ctcgcggagg ccaccaacat cattgccgaa ggcgaagtcc tgcaactgat
     1741 gaacatgcac gacgcatcgc tggacgaagc cgattacctg cgcgtcatcc gctcgaaaac
     1801 agccaaattg ttcgaagcca gcgctcgact gggagcgatc ctgtcggcca gtgggccggc
     1861 gttcgaagaa gcctgcgcca tttatgggca ggcgctgggc accgcgttcc aggtcgtcga
     1921 cgatgtgctg gactatgcag gaaccgcggc ggaaatgggc aagaatctgg gcgacgacct
     1981 gcgcgaaggc aaagccacct tgcccttgat catggccatg cagttgggca caacaagcca
     2041 gcgcgacctg atccgcgaag cgattgaaac cggggctgtg acccaattgt cgttggtggt
     2101 gaatatcgtc cggcagaccg gtgcgctgga cgccacccac gatgccgcgg cacaggaagc
     2161 gcaacgggca attgctgccg cgcggactct ccccgacaat cgattcaccg agggtttgat
     2221 acaattggcc agtcagctac tggaacgcag gcactgagtt tctccatcgt gttccagcca
     2281 tcggggtgta gcttagcctg gtagagcgct acgttcggga cgtagaggcc ggaggttcga
     2341 atcctctcac cccgaccaca tattcaactt tgttcacagc ctcaccagtg aacattgcga
     2401 cactgttttc cggtttacag tctgagggca atcggcgaag attgccctga tgtcactcat
     2461 cgagaaaccg tcgaacttca tggctgttgc agaaccccct tccaaagata tatcggccgt
     2521 cgcgcttccg ggcttggcac gcgcattggt taccgcggga aagctcggtc agaaagctgc
     2581 cgaagacatt taccgtaagg cgcttaccgg caaaaccaac ttcatttcag agttgacagg
     2641 atccggggcc gtatctgcgt ccgacctcgc ccacaccatg tcaacggcat ttgcggtccc
     2701 tctggtggac ttggacgccc tcgatcgcaa ccgacttcca aaaggtctgc tcgaccccaa
     2761 gacatgccag gattttcgcg tgctcgtcct gagcaagcgg ggaaaccgtc tcatcgttgc
     2821 cacagccgat cctgccgatc aggcagctgc ggagaaaatc aagttcgcca cccaaatggg
     2881 tgtggactgg gtgatcgccg agttcgacaa gctttcccgg atggtcgaac agagcacgac
     2941 ttccgtcaac gaagcgatgg aaagtttcgt cggcggggaa tttgagtttg acgaatcgtc
     3001 gatggacgcc tctgccgccg atgctggcga ggtggacaca tcagaagttg acgacgcacc
     3061 catcgtcaag tttctccaca agatgctgct ggatgcgttc aatatgcgcg cttcagacct
     3121 gcactttgaa ccctatgagt tcaactaccg ggtacgtttc cgggtcgatg gcgagttgag
     3181 ggaaatcgct tcccccccca tcgccatcaa ggacaagctg gcgtcacgca tcaaggtgat
     3241 ttcacgactg gacatctccg aaaaacgggt cccgcaagac ggacgcatga aactgaaggt
     3301 cggacctgac cgatcggtcg acctgcgcgt cagcacgctg cccaccttgt tcggcgagaa
     3361 gatcgtgatc cggattctcg accccagcag tgccaaactc gggattgacg ccttaggg
//
LOCUS       DAORPM010000258         3415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9242_length_3415_cov_9.636310, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000258 DAORPM010000000
VERSION     DAORPM010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9242_length_3415_cov_9.636310"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLD53_10715"
     CDS             <1..359
                     /locus_tag="PLD53_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC61431.1"
                     /translation="DTLWGIARRLTSDGRNWPQLLSGHNRAVELGLAVGSRITDPGRI
                     RPGQKIYVPLSPAAGFRAIEYHVVRGESLSGIALRIYGDAQMWRAIYRDNERQLPNPD
                     LIQPGQVLILRPRVPQ"
     gene            356..826
                     /locus_tag="PLD53_10720"
     CDS             356..826
                     /locus_tag="PLD53_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRC61432.1"
                     /translation="MSLQSVRDFFSARNMDMSIIELEVSTATVELAAQAHGVEPGRIA
                     KTLSFRLGDGRVIILVARGDARIDNRKFKAVFGKGKMSPPEEVVALTGHPVGGVCPFG
                     LAQPLPIYLDKSLQDFDEVLPAAGAIHTAVRISPALLAAVTDGQWVDVCLLRAP"
     gene            complement(879..3119)
                     /locus_tag="PLD53_10725"
     CDS             complement(879..3119)
                     /locus_tag="PLD53_10725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC61433.1"
                     /translation="MTRSFDFVVVGGGLAGATAVETLRAEGAEGGILLLGAERHLPYQ
                     RPPLSKLAITAERAPPPRLILSQSRYRELDIDLVLDSPVTAVDSKRQMLHTLPGKKIH
                     YQKLLIATGASPCRLSLPGAALPGIFHLYSLTDAESIRSTAHKGLHAVVIGGSFIGLE
                     VAASLRQRGLAVTLIERGVLLNKLHNPEMSSFFKRAFEAQGVEVIVGDAPAMFQGEAA
                     VEAVVTQAGRTIACDMVVIGAGVSPETGFLRGSGIEVGDGIVVDRFLQTTQPNVFAAG
                     DVANFFDPVFNRQHRIEHWDNAIKQGKLAARNMLGQRLPYDEVPYFYSQMFDLSFNLL
                     GLFESGDEKVERGSLQAGSFAAFYLRDDVPRALFSLGRPTDETKVVELLIKHRVNLKS
                     SKPSLSDPDYQLSHIPNQTIYILQGGGAFGGFECGAVRALEESGIRPDVVAGVSIGAF
                     NGAIIAGNPDRAADALASFWNDISTLSPDLPDESLRQQVACGLIAMFGVPQFFRPRWF
                     MPMLSLEQMPSHWSSLYDTTPAIKLLEKYVDFSKLKSSPVRLMVSAVDIQTSELVVFD
                     SYVDDLTPEHIIASGSLPPGFPWTTIDGKHYWDGGIVSNSPLDLVVKRCGSAGKRVFI
                     IDLFPGKRNALPGNLAEAMARQSEILYCERIRSDVKTRDLIRDFRKLVDEIVAEMPAD
                     TVARLRHRPRFIEMMGEDAPMTITRIVRENSEGEPSSKDYDFSRQTIDQLIESGYRMT
                     RQALGL"
     gene            complement(3188..>3415)
                     /locus_tag="PLD53_10730"
     CDS             complement(3188..>3415)
                     /locus_tag="PLD53_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61434.1"
                     /translation="LLAPSTRVPRGLLLPAPGGVIREEEVPREGVRLLRERVLACSSN
                     GTTYIWTRRRREVGRGEGSSGLMFDEVEAQP"
BASE COUNT          690 a         1031 c         1008 g          686 t
ORIGIN      
        1 gcgatactct gtggggcatt gcccgtcggc tgaccagcga cggccgcaac tggccacaac
       61 tgctgagcgg ccacaaccgc gcagtcgaac tgggtttggc cgtcggtagt cggatcaccg
      121 atccagggcg catccgtccc ggccagaaga tctatgtgcc gctgagtcca gcggccggtt
      181 tcagggcaat cgagtaccac gtcgttcgcg gcgaatccct ctcgggcatc gccttgcgca
      241 tctacggcga tgcgcaaatg tggcgggcca tctatcgcga caacgagcgg cagttgccga
      301 accccgactt gatccagccg ggacaggtat tgatcctgag accgcgtgtg ccgcaatgag
      361 cctgcaatcc gtacgagatt tcttttctgc ccgcaacatg gatatgtcca tcatcgagct
      421 ggaggtcagc accgccaccg tcgaactcgc cgcgcaagcc cacggcgtgg aacccggccg
      481 tatcgccaag acgctctcgt ttcgcttggg cgatggtcgc gtcatcattc tggtggcacg
      541 cggcgacgca cgcatcgata accggaaatt caaggctgtt ttcggcaagg ggaagatgtc
      601 gccgccggaa gaggtggtcg cactcaccgg gcaccccgtc ggcggtgtct gcccgtttgg
      661 gctggctcag ccactgccga tctatctgga caaatccttg caggatttcg atgaagtctt
      721 gcccgccgcc ggcgccatcc acaccgcagt gcggatcagc ccggcgctac tggccgcagt
      781 gaccgacggc cagtgggttg acgtttgcct gctgcgcgct ccttgattca gctgccatag
      841 cgatgaagat tcggtcaaat tcggacgatt cagcccactc aaagccccaa cgcctggcgg
      901 gtcatgcggt aacctgactc gatcagttgg tcgatggtct gtcgtgaaaa atcatagtct
      961 ttcgatgaag gttcgccttc gctgttttcc cggacaatgc gggtgattgt catcggcgcg
     1021 tcttcgccca tcatttcgat gaagcgtggc cgatgccgga ggcgggcaac ggtatcggca
     1081 ggcatttcgg cgacaatttc atcgaccagt ttccggaagt cgcgaatcag gtcgcgagtc
     1141 ttcacgtcgc tgcggatgcg ttcgcagtag agtatttcgc tttgcctggc catggcttcc
     1201 gccaggttgc cgggcaaggc atttctcttg ccgggaaaca ggtcgatgat gaagacgcgc
     1261 ttgccggcag aaccgcagcg tttcaccact aggtccaacg gcgaattgct gacgattccg
     1321 ccatcccaat agtgtttgcc atcgatagtc gtccacggga atcctggcgg taaactgccg
     1381 ctggcgatga tgtgttcggg ggtcaggtcg tcaacgtagc tgtcgaaaac aacgagttcg
     1441 ctggtctgga tatcgaccgc actcaccatc agccggaccg ggctcgattt cagcttgctg
     1501 aaatcaacgt acttttccag cagtttaatc gccggcgttg tgtcgtaaag gctggaccag
     1561 tggcttggca tctgttccag actgagcatc ggcatgaacc agcgcggcct gaagaactgg
     1621 ggaacgccaa acatggcgat cagcccgcaa gcgacctgct ggcgtagact ctcgtccggc
     1681 aagtcaggcg acagggtgct tatatcgttc cagaaagacg ccagtgcatc ggcggctcga
     1741 tcggggttgc cggcaatgat tgcgccattg aaggcgccaa tcgagacgcc ggcgaccaca
     1801 tccgggcgaa tgccggattc ttccagcgct ctcactgcgc cacactcgaa tccaccaaag
     1861 gcaccgccgc cctgcagaat gtagatcgtc tggttgggga tatgactcag ctggtagtct
     1921 ggatctgaca gactcggttt gctggacttg agattcaccc gatgcttgat gagaagttca
     1981 acaaccttgg tctcgtccgt cggcctgccg agggaaaaga gggcgcgggg cacatcgtcc
     2041 cgcagataga aggctgcgaa cgatccggcc tgtaaggatc cacgctcgac tttttcatcc
     2101 ccggattcga aaaggcccag caggttaaag ctcagatcga acatctgact atagaaatag
     2161 gggacctcgt cgtaggggag gcgttggccg agcatgttcc gggccgccag ttttccctgc
     2221 ttgatggcat tgtcccagtg ttcgatacgg tgctgccggt tgaaaaccgg gtcgaaaaag
     2281 ttcgcgacat caccggcagc aaaaacattg ggctgcgtgg tttgcagaaa gcgatcgacc
     2341 acaataccgt cgccaacctc gatgccgctt ccccgcagga aacctgtttc agggctcacg
     2401 ccggcgccga tcaccaccat atcgcaggca atggttctgc cggcctgagt caccaccgct
     2461 tcaactgcag cttcgccttg gaacattgcc ggcgcgtcac cgacgataac ctcgacccct
     2521 tgcgcttcga aggcgcgctt gaaaaaggac gacatctccg gattgtgcaa tttattgagc
     2581 agcaccccgc gctcgatcag ggtcaccgcc aggcctcttt gccgcagtga agcggcaacc
     2641 tccaggccaa tgaagctgcc gccaataacc acggcgtgca gtcccttgtg cgcagtcgac
     2701 cggatcgatt cggcatcggt cagcgagtaa agatgaaaaa tgccaggaag cgccgcaccg
     2761 ggcagagaca aacggcaggg cgatgcgccg gtcgcgatca gcaacttctg atagtggatc
     2821 ttctttccag gcaaggtgtg gagcatctgc cgctttgaat caactgccgt gacgggacta
     2881 tccaggacga gatcgatgtc cagttcgcgg tacctgctct ggctaagaat gagccggggc
     2941 ggcggcgccc gctcggcagt aatggcaagc ttcgatagcg gtggccgttg ataggggaga
     3001 tgccgttctg ctcccaggag gagaatgcct ccctcggcgc cctcggctct caaggtttcc
     3061 acggccgtag caccggccag cccgccaccg acgacgacaa aatcgaaact ccgtgtcatg
     3121 tatgaattct cgctgatctg cttgccgtgt cattggggcg taatgtcacc tgcgcaccaa
     3181 gaatggatca gggctgcgcc tccacctcat cgaacatcaa cccgctgctt ccttctccac
     3241 ggccaacctc gcgtcgacgg cgggtccata tgtaggttgt tccgttactg ctgcatgcca
     3301 gtacgcgctc gcgcagcagg cgcacaccct cgcgagggac ctcctcctcg cggatgacgc
     3361 cgccgggcgc gggtagcagc aagccacgcg gaaccctggt agacggcgca agcag
//
LOCUS       DAORPM010000259         3414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9248_length_3414_cov_14.647216, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000259 DAORPM010000000
VERSION     DAORPM010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9248_length_3414_cov_14.647216"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1984)
                     /gene="hscA"
                     /locus_tag="PLD53_10735"
     CDS             complement(80..1984)
                     /gene="hscA"
                     /locus_tag="PLD53_10735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HRC61435.1"
                     /translation="MALLQIAEPGESAAPHQHRLAVGIDLGTTNSLVATVRSGLANVI
                     SDELGRPLLPSVVRYHEDGRAVVGYEAQAEQAHDPRNVIASVKRFMGRSIKDIAHRES
                     MPYDFEDGQGMVRVRTKAGTKSPVEISADILRVLRLRAEASVGGPLVGAVITVPAYFD
                     DSQRQATKDAAKLAGLSVLRLLNEPTAAAIAYGLDNAAEGVYAVYDLGGGTFDISILN
                     LSKGVFEVMATGGDSALGGDDFDQRIFCWILEAAKLKPLSIEDARQLLTCAREAKEYL
                     TLHGVAPITARLNSGEVVDLRLTTGIFTEITQTLVAKTIQPVKRALRDAGLSGSEVKG
                     VVMVGGATRMPQVQRAVGEFFKQEPLNNLDPDKVVALGAAMQANLLAGNRAAGDDWLL
                     LDVIPLSLGLETMGGLVEKIIPRNSTIPVARTQEFTTYRDGQTALAVHVVQGERELVS
                     DCRSLARFELRGIPPMVAGAPRIRVTFQVDADGLLSVSARELTSDVEAGITVKPSYGL
                     SDDEIAGMLKDSMTHGKDDALRRALKEAQVEAQRLIEAVQSALKSDSDLLSKDQLEKI
                     EASIAKLQTTALGDNRRHITLAMDDLEADTKDFAARRMDKSIRRAFSGKSINELDAGA
                     NSSKIQGETV"
     gene            complement(2023..2550)
                     /gene="hscB"
                     /locus_tag="PLD53_10740"
     CDS             complement(2023..2550)
                     /gene="hscB"
                     /locus_tag="PLD53_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HRC61436.1"
                     /translation="MNFSDDHFALFELPRTFRLDRTALDARYRAIQAQIHPDRFANAG
                     DAERRLSLQWATRINEAYQTLKKPMSRARYLLHLAGQDVDAESNTAMPTEFLVEQMEW
                     RESVAEARAAGNHRELEHLQHRLKHDIDECYAEIATLFDDHRDLEQVAEKVREVMFLD
                     KLLFEIDDAMAALEA"
     gene            complement(2673..2996)
                     /gene="iscA"
                     /locus_tag="PLD53_10745"
     CDS             complement(2673..2996)
                     /gene="iscA"
                     /locus_tag="PLD53_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HRC61437.1"
                     /translation="MAVTLSEKAAKHVSSYLVKRGKGIGLRLGVRTSGCSGVAYKLEF
                     ADAADPDDIEFESHGVKVLIDAKSLPYLDGTELDYTREGLSEGFKFNNPNVKDACGCG
                     ESFNV"
     gene            complement(3031..3414)
                     /gene="iscU"
                     /locus_tag="PLD53_10750"
     CDS             complement(3031..3414)
                     /gene="iscU"
                     /locus_tag="PLD53_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRC61438.1"
                     /translation="MSYSIKVLDHYENPRNVGSFGKEEDGVATGMVGAPACGDVMKLQ
                     IKVGKDGIIEDAKFKTYGCGSAIASSSLVTEWVKGKSIDQALSIKNTQIADELALPPV
                     KIHCSILAEDAIKAAVADYKKKHIE"
BASE COUNT          731 a         1043 c          875 g          765 t
ORIGIN      
        1 cagacggatt cccctgccct ggcctcgatc accgcaccct ccgggcaaag ctcgatatgc
       61 ggcagaacga tgatctgcgt cacaccgttt ctccctggat ctttgaagaa tttgcgcctg
      121 cgtcaagttc attgatgctc ttgccggaaa atgcgcggcg aatcgactta tccatgcgtc
      181 gcgcggcaaa atccttggta tccgcctcaa gatcgtccat ggccaaggtg atgtgccggc
      241 gattgtcacc aagcgccgtc gtctgtaact tggcgatgga ggcttcaatc ttttccagtt
      301 gatctttcga caacaaatca ctgtcgctct tcagtgccga ctgaacggct tcgatcaaac
      361 gctgtgcttc gacctgcgct tccttcaagg cccgcctgag cgcatcatcc ttgccatgcg
      421 tcattgaatc cttgagcatg cccgcaattt cgtcatcgga gagcccatac gagggcttga
      481 cggtaatgcc ggcttcgaca tcggaggtca gttcacgtgc cgaaacggaa agcaaaccat
      541 ccgcatcgac ctggaaggtc acgcgaattc gtggcgcacc agctaccatc ggcggtatgc
      601 cgcgcagctc aaagcgcgcc agcgaacggc agtcgctgac cagttcgcgt tcgccttgca
      661 caacatgcac cgccaatgcc gtctggccat cgcgataggt ggtgaattcc tgagttcgcg
      721 ctaccggaat cgtcgagttg cgcgggatga ttttttcaac cagaccgccc atggtttcca
      781 gcccgagcga gagcggaata acgtcgagca acagccagtc gtcaccggca gcccggttgc
      841 cagccagcag attggcctgc atcgccgcgc ccaaggcgac aaccttgtcc ggatcaagat
      901 tgttcaaggg ctcctgcttg aaaaattcgc cgacagcacg ctgtacctgc ggcatgcgcg
      961 tagcgccgcc aaccatgacc acacccttga cttccgaacc tgacaacccg gcatcgcgca
     1021 atgctctctt gaccggttga atggtcttgg caaccagcgt ttgcgttatt tcggtgaaaa
     1081 ttccggtcgt cagtctcaga tcgaccactt caccgctgtt cagccgtgcc gtgatcggcg
     1141 caacgccgtg cagggtaagg tactccttgg cttcacgggc acaggtcaga agttggcggg
     1201 catcctcgat cgataacggc ttgagtttgg cggcctcgag aatccagcag aaaatgcgct
     1261 gatcgaagtc atcacccccc agcgccgaat cgccacccgt ggccatgacc tcgaacacgc
     1321 ccttggacag attgaggatc gagatgtcga acgtgccgcc acccaggtcg taaacggcat
     1381 agactccctc ggccgcgtta tccaggccat aggcaatggc ggctgcggtc ggttcattca
     1441 gcagacgcag aaccgaaagc ccggccagct ttgccgcgtc cttggtcgcc tgacgctgtg
     1501 agtcatcgaa gtaggcagga acggtaatca ccgctccgac cagagggcca cccaccgacg
     1561 cttccgcgcg cagacggaga acgcgaagaa tgtcggctga aatctcaacc gggcttttag
     1621 ttcctgcctt ggtgcgcacg cgaaccattc cctgcccatc ttcgaagtca tagggcatcg
     1681 attcgcgatg ggcgatatcc ttgatgctac gccccatgaa gcgcttgacc gacgcaatga
     1741 cattgcgcgg gtcgtgcgcc tgctcggcct gagcttcgta gcccacaact gcacgaccat
     1801 cttcgtgata gcgcaccacc gaaggaagca gtggacggcc cagttcatcg ctgatcacat
     1861 tggcgagacc actgcgaacc gtagccacca gcgagttcgt ggtgcccaaa tcgattccga
     1921 ccgctagccg gtgctggtgt ggcgcagccg attccccagg ttctgcaatt tgtaagagtg
     1981 ccatgaaatt caggtgcccc gtcttgtagg attttgctct ttttaagctt caagcgccgc
     2041 catggcgtcg tcgatctcga ataacaattt gtcgagaaac atcacttccc gcactttttc
     2101 agccacctgt tcaagatcgc gatgatcgtc gaacaaggtt gcgatttccg catagcattc
     2161 gtcgatgtcg tgcttcaagc gatgctgcag atgctcaagt tcgcgatgat tcccggcagc
     2221 gcgtgcttcg gcaaccgatt cgcgccattc catttgttca acgagaaatt cggtcggcat
     2281 cgccgtgttg ctttcggcat caacatcctg cccggccaga tgcaacagat atcttgctct
     2341 ggacatgggt ttcttcagtg tctgataggc ctcgttgata cgcgttgccc actgcaacga
     2401 aagtcgccgt tccgcatcgc ctgcattagc gaatctgtcc ggatgaatct gcgcctgaat
     2461 cgcgcgataa cgtgcatcca gtgccgttct gtcaaggcgg aaagtgcgcg gcaactcgaa
     2521 cagcgcaaaa tgatcgtcag agaaattcat tcggtttttc tgcggtcttg ccgcatctac
     2581 caatcagcca agccggggcc agttagccgc gatcaacaaa cacgaacaga acggcaaacg
     2641 ttgtatatca acgccccggc tcgccgccgc catcaaacgt taaagctctc gccgcaaccg
     2701 caggcatcct tcacattcgg gttattgaat ttgaagcctt cgctcagacc ttcacgcgtg
     2761 taatcaagct ccgttccgtc aaggtaaggc aggctcttcg catcgatcag cactttgacg
     2821 ccatgcgatt caaactcgat gtcatccggg tcggctgcgt cggcaaattc gagtttgtag
     2881 gctacgcccg agcaaccgct ggtgcgaaca ccgaggcgaa gaccgatgcc cttgccacgc
     2941 ttgaccaagt agctagaaac atgttttgcc gctttttcgg aaagcgtaac cgccattgca
     3001 atactccttc ttttctctag taattccgct ctattcaata tgcttcttct tgtagtcggc
     3061 cactgccgcc ttgattgcgt cctcagcaag aatagaacag tgaatcttca ccggcggcaa
     3121 agccagttcg tcggcaatct gggtattctt gatgctcaac gcctgatcaa tgctcttgcc
     3181 cttgacccac tcggtaacca gcgaagacga ggcgatcgcc gaaccacaac cataggtctt
     3241 gaacttggca tcctcgatga tgccgtcctt gccaaccttg atctgcagtt tcatcacatc
     3301 gccgcaagcc ggtgcaccga ccatgcccgt tgccacacca tcctcttcct tgccaaagga
     3361 acccacgttg cgcgggtttt cgtagtgatc cagaactttt atgctgtatg acat
//
LOCUS       DAORPM010000260         3307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9702_length_3307_cov_5.973862, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000260 DAORPM010000000
VERSION     DAORPM010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9702_length_3307_cov_5.973862"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLD53_10755"
     CDS             complement(<1..578)
                     /locus_tag="PLD53_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toluene tolerance protein"
                     /protein_id="HRC61439.1"
                     /translation="MKQLKQQDYFAMREGAEVLEADRHGDKVLRLADGSILKIFRRKR
                     LITSAALYPYAQRFANNAETLAKLGIPVPRIIAVGRIQEIARDAVHYEPLAGKTLREM
                     LRAGMLPERKRELKAVFTRFVIRLHDKGIYFRSLHLGNVVCTPEGELGMIDFSDLRIH
                     HWPLGKYLRARNMRRMQGIADEVDWLDLGAIV"
     gene            711..2591
                     /gene="msbA"
                     /locus_tag="PLD53_10760"
     CDS             711..2591
                     /gene="msbA"
                     /locus_tag="PLD53_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HRC61440.1"
                     /translation="MQQANPPLSSSLKIYLRLLGYLRPFIGLFVVSIIGYLIFASSQP
                     MLAAVLKYFVDGLAASPEATRHSLPLIGRVELMYGIPILLVVIIVWRSVGSFLGNYYL
                     ARVSLGLINDLRIALFNSLLRLPNRYFDQQSSGHLISRITYNVTMVTGAATDAIKVVI
                     REGLTVAFLFGYLLWMNWKLTAIMLAILPVIAIMVSSVSKKFRKQSKKIQASMGDLTH
                     IASETIQGYRVVRSFGGEDYESRRFKAASEDNTAKQLRMVKTGATFTPSLQLVTFSAM
                     AVILFLVLLLRGDASAGDLVAYISAAGMLPKPIRQLSEVSSTIQKGLAGAESIFAQLD
                     ETPETDLGPVTRDKISGRIEIKNLNFHYPGSEQQVLCDVNLVVEPGQMVAIVGRSGSG
                     KSTLVSLIPRFYHHEDGQILIDGIDVKDFTLRNLRRHIALVTQQVVLFNDTIAANIAY
                     GDLALAPRAEIERAAEAAYANEFIDRLPLGFDTLVGENGVLLSGGQRQRLAIARAILK
                     DAPILILDEATSALDSESERHIQKALDHVTANRTTLVIAHRLSTIEKADVIMVMDQGR
                     IVERGTHAELLALNGHYASLHAMQFSEGVSPGSLVNAGPEASPVALEVDITRADKSGA
                     QR"
     gene            2710..>3307
                     /locus_tag="PLD53_10765"
     CDS             2710..>3307
                     /locus_tag="PLD53_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRC61441.1"
                     /translation="MKILVTGAAGFIGLHVSRRLLERGDEVVGIDNLNDYYDPRLKHA
                     RLDTLNSFANFRFVRMDVADAAALGSLCAIEKFNRVIHLAAQAGVRYSLTNPQAYIQS
                     NLVGFANVLEACRHHGVEHLVYASSSSVYGANTRMPFSVHDEVNHPVSLYAATKKSNE
                     LMAHSYSHLFGLPTTGLRYFTVYGPWGRPDMSPWLFTSA"
BASE COUNT          708 a         1032 c          888 g          679 t
ORIGIN      
        1 ctgacgatgg cgcccagatc cagccagtcg acctcatcgg cgatgccctg catacgacgc
       61 atgttgcggg cccgcaggta tttgccaagc ggccagtgat gaatgcgcag gtcggaaaag
      121 tcgatcattc cgagctcacc ctcgggagta cacaccacgt tcccgagatg caatgaacgg
      181 aagtagatac ccttatcgtg caaccggatc acaaaccggg taaagaccgc tttgagttcc
      241 cgcttccgct cgggcaacat gcccgcgcgc aacatctcac gcaaagtctt tcctgccagc
      301 ggctcgtaat gaaccgcatc gcgggcaatt tcctggatgc ggccgacagc gatgatcctc
      361 ggcaccggga tacccagctt cgccaaggtc tcggcattgt tggcgaagcg ctgggcatag
      421 gggtaaaggg cggcggaagt aatcaaccgc ttgcgccgga atatcttgag aatgctgcca
      481 tcggcaaggc gaagaacctt atcgccatgc cggtccgcct ccagaacttc tgcgccttcg
      541 cgcatggcga agtaatcctg ctgctttagt tgcttcaagt cactgccaat cgaaaaaccg
      601 ctagtttacc gttttgccgg caaagcgggg agccgggcaa ttccaggcga catgttaaac
      661 tgcggcctcc ggaccacggg tccgccagtc gcaccggaag gccttcaccc atgcagcaag
      721 ccaacccgcc gctcagttca tcgctgaaga tctatctccg gctgctgggt tatctgcgcc
      781 ctttcatcgg gttgtttgtc gtcagcatca tcggttatct gatctttgcc tcatcgcaac
      841 caatgctcgc ggcagtactg aaatacttcg tcgatggtct ggccgcatcg ccggaagcaa
      901 cccgccatag cctaccgctg attggccggg ttgaactgat gtatggcatc cccatcctgc
      961 tggtggtgat tatcgtctgg cgcagtgttg gctcatttct cggcaactat tacctcgccc
     1021 gtgtctcgct gggtctgatc aacgatcttc gcatcgcgct cttcaacagc ctgctacggc
     1081 tgcccaatcg atattttgac cagcagagtt ccggccacct gatctcccgc atcacctata
     1141 acgtcaccat ggtgacgggc gctgcgaccg atgccatcaa ggtcgtcatt cgcgagggac
     1201 taaccgtcgc tttcctattc ggttacctgc tctggatgaa ctggaaactc actgcgatca
     1261 tgctggccat cctgcccgtg atcgccatca tggtcagtag cgtcagcaag aagttccgca
     1321 agcaaagcaa gaaaatacag gcgtcgatgg gtgacctgac ccatatcgcc tcggaaacca
     1381 tccagggcta ccgcgttgtg cgcagctttg gcggcgagga ctatgaatcg cgccgtttca
     1441 aggcggccag cgaggacaac accgccaagc agttacgcat ggtcaagact ggcgcgacgt
     1501 ttacaccttc actgcaactg gtgactttct cggccatggc cgtgatcctc tttctggtat
     1561 tgctgctgcg cggcgacgct tccgccggag atctggttgc ctacatttca gccgccggca
     1621 tgttgccgaa accaatccgc cagctctcgg aagtcagctc gacgatccag aaaggactgg
     1681 ccggcgccga gagtatcttc gcacaacttg atgaaacgcc ggaaacggac ctcggcccgg
     1741 tgacgcgcga caaaatcagc ggccgcatcg aaatcaaaaa cctcaacttc cattatccgg
     1801 gcagcgagca acaggtgctt tgtgacgtca atctggtcgt cgaaccgggg cagatggttg
     1861 ccatcgtcgg ccggtcaggt agcggcaagt cgaccctggt cagcctgatt cctcgtttct
     1921 atcatcacga ggacggccag atcctgatcg acggtatcga tgtcaaggat ttcacgctac
     1981 gcaacctgcg ccgccacatc gcactggtaa cgcaacaggt tgttcttttc aacgacacga
     2041 ttgccgccaa catcgcctac ggcgacctcg ctctggcacc acgcgcggaa atcgaacgtg
     2101 ctgccgaagc ggcctacgcc aacgagttta tcgatcgact gccactgggg ttcgacacgc
     2161 tggtcggcga gaacggcgtc ctgctctccg gcgggcagcg acaacgtctg gcgatcgccc
     2221 gcgccatctt gaaagacgcg cctattctca ttctcgacga agccacatcg gcactcgact
     2281 ctgaatccga gcgccatatc cagaaagcac tggatcacgt cacggccaac cggacaacgc
     2341 tggtcatcgc ccatcgtttg tcaaccatcg agaaggcaga tgtaatcatg gtcatggacc
     2401 aggggcgtat cgtcgaacgc ggcacccacg ccgaattact tgcgctcaac ggtcactatg
     2461 cgagccttca cgccatgcag ttcagcgaag gtgtttctcc tggaagcctc gtcaatgctg
     2521 gcccggaggc ttcgccagta gcccttgaag ttgatatcac gcgcgcggat aagtccggag
     2581 cacaacgatg aaaacaagca ttgtcgaggc caataatatt gcaccgttca acaccgcctt
     2641 gtgcaaggca agcaatggga agttgcctcc cttgcgggga cccaaaaaca tcccgaagga
     2701 tcgactgccg tgaagattct cgttaccggc gccgccgggt tcatagggct gcatgtctcc
     2761 cgtcgacttc tggagcgcgg cgatgaagtc gtcggcatcg acaacctgaa cgactactat
     2821 gatcccaggc tcaagcacgc gcgcctcgat accttgaatt cgttcgccaa cttccgcttc
     2881 gtacgcatgg atgtcgccga tgcagccgca ctaggaagcc tgtgcgccat cgaaaagttc
     2941 aaccgggtca tccacttggc ggcgcaagcg ggtgtgcgct attcactgac caacccgcag
     3001 gcgtacatcc agagcaatct ggtcggtttc gccaacgtgc ttgaagcctg tcggcaccat
     3061 ggcgtcgaac atctcgttta tgccagcagc tcaagcgtct acggcgccaa tacccggatg
     3121 ccgttttcgg tgcatgacga agtcaaccac ccggtcagcc tctatgccgc gacaaagaag
     3181 tcgaacgaac tgatggcgca cagctacagc cacctgttcg ggctaccgac caccggcctg
     3241 cgctacttca cggtctatgg cccctggggc cgtccggata tgtcgccctg gctgttcacc
     3301 agcgcca
//
LOCUS       DAORPM010000261         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9770_length_3295_cov_9.029321, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000261 DAORPM010000000
VERSION     DAORPM010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9770_length_3295_cov_9.029321"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="bioA"
                     /locus_tag="PLD53_10770"
     CDS             complement(<1..1000)
                     /gene="bioA"
                     /locus_tag="PLD53_10770"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HRC61442.1"
                     /translation="MRNSDLLQRSLKAVWHPCTQMKQHTDQGAGSLPLIPISHGKGAW
                     LHDFDGNRYLDGISSWWVNLFGHCNPRINAALRDQLEQLEHVILAGCTHRPVVELSER
                     LSALTGGALGHCFYASDGASATEIALKMSFHSWRNRGREKKQDFLCLEGSYHGETVGA
                     LAVTDVAIFRDAYAPLIRPAATVPTPDARKARDGETPADVARRAAAALEQHLDQHHES
                     IAALIVEPLIQCAGGMAMYDPDYLRRARALCDHFEVHLICDEIAVGCGRTGTFFAYEQ
                     AGIKPDFLCLSKGISGGYLPLSIVLTTDAVYGAFYEDATARGFLHSHSYTGNALACR"
     gene            complement(1010..2185)
                     /locus_tag="PLD53_10775"
     CDS             complement(1010..2185)
                     /locus_tag="PLD53_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HRC61443.1"
                     /translation="MKRSRPSSRKRISDDANELVRLATGLAESGGRLEAKLWDKQLTE
                     LVCKLLHDGAEDDLNASLDRLFDSHALAHDSLADILESSAESCVMSLQGQDYDILLFN
                     VPLLVWSRFSIPAGTIAKSALQTLKTQLGAHLFSANAQMALADYLYSPDQLPRTYVDT
                     WLLMRELGGAALCNSPVKLDMSSLPETNQFLSDARYLIGAIAVPRGMPLFRWNESDRS
                     TRETALREWVKQGSPCLEPQLTGCAFQLLIADAYHAACRNADMASRPYSLRASVAFLQ
                     TTLGKPAEALRAVIGGFHDKRLEEYRIGFGPRDSDNLFHGVVWPLLGAEDETTEVTGE
                     IEGILRETGIKEVLVHSQRFPFEFCDDCGAPLYPNTEGETVHAELPEQSNAPSQTLH"
     gene            2382..>3295
                     /locus_tag="PLD53_10780"
     CDS             2382..>3295
                     /locus_tag="PLD53_10780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRC61444.1"
                     /translation="MLKASESFRDIRDAVRDLCAQYPAAYFREIDRERSYPEAFVDAL
                     TRAGWLAALIPQEYGGSGLGLTEASVILEEINRCGGNSGACHGQMYNMGTLLRHGSDA
                     QKQAYLPRIASGELRLQSMAVTEPSTGTDTTRIKTVAVKQGDRYVVNGQKVWISRVQH
                     SDLMILLARTTPLAEVSRKSEGMSIFIVDLRQAIGQGMTVQPIPNMVNHETNELFFDN
                     LEIPVENLIGEEGKGFRYILDGLNAERALIAAECIGDGYWFIDKVSSYVKERVVFDRP
                     IGQNQGVQFPIAEAYIEVEAASLMRYEA"
BASE COUNT          639 a          974 c         1050 g          632 t
ORIGIN      
        1 cgcggcaggc cagcgcgttg ccggtgtagg aatgcgaatg caggaagccg cgcgctgtgg
       61 cgtcttcata aaacgcgcca tagaccgcat cggtggtgag cacgatggac agcggcaggt
      121 aaccgccgga gatgcctttc gagagacaga ggaaatccgg ttttatcccg gcctgctcgt
      181 aggcgaagaa agtgccggtg cgaccgcagc cgacggcgat ctcgtcgcaa atcaggtgca
      241 cctcgaagtg gtcgcagagc gcccgagctc gacgcaggta gtccgggtcg tacatggcca
      301 tgccgccggc gcactggatc agcggttcga caatcagtgc ggcgatgctt tcgtgatgct
      361 gatccagatg ttgttcgagc gcggcggcag cgcgacgcgc aacgtcagcc ggcgtctcgc
      421 catcgcgtgc tttgcgcgcg tcgggggtcg gtacggtggc ggccgggcgg atcagcggtg
      481 cgtaggcgtc cctgaaaatc gccacgtcgg taacagccag cgcgccaacg gtttcgccat
      541 ggtagctgcc ttcaaggcag aggaaatcct gcttcttctc gcggccgcga ttgcgccaac
      601 tgtggaagga catcttcagt gcaatctcgg tcgccgacgc gccgtccgag gcgtagaagc
      661 aatgccccag tgcgccgccg gtcaacgctg aaaggcgttc cgacagctcg accaccgggc
      721 ggtgcgtgca gccggcgagg atgacgtgtt caagctgttc gagctgatcg cgcagggcgg
      781 cattgatgcg cgggttgcag tggccgaaca gattgaccca ccacgagctg atgccatcca
      841 gatagcgatt gccatcgaaa tcatggagcc aggcgccctt gccgtggctg atggggatca
      901 gcggcagcga tccggcgccc tggtcggtgt gctgcttcat ctgcgtgcag ggatgccaga
      961 cggctttcag gctgcgttgc agcaggtcgg aatttcgcat cggcggcgat caatgcaagg
     1021 tttgcgaggg tgcgttgctc tgctccggca attcggcgtg taccgtttca ccttcggtgt
     1081 tggggtagag gggggcaccg caatcgtcgc aaaactcgaa ggggaagcgc tggctatgca
     1141 ccaggacttc cttgataccg gtctcgcgca ggatgccttc aatttcgccg gtaacctcgg
     1201 ttgtttcatc ctcggcgccg agcagcggcc agacgacgcc gtgaaaaagg ttatcgctgt
     1261 cacgcggccc gaatccgatt cggtattcct ccagacgctt gtcatggaat ccgccaatca
     1321 cggcgcgcaa tgcttcggca ggtttgccca gcgtggtttg cagaaaggcg accgacgcgc
     1381 gcagggagta cggccgcgag gccatgtcgg catttcggca cgcggcgtgg taggcatcgg
     1441 cgatcagcag ttggaatgcg catccggtca gttgcggctc cagacagggg cttccctgct
     1501 tcacccactc tcggagcgcc gtttcgcgag tggaacgatc cgattcattc cagcggaata
     1561 gcggcatgcc gcgcggtacg gcaatggccc cgatcagata gcgtgcgtcg gaaaggaatt
     1621 gattggtttc cgggagcgat gacatgtcga gcttcacggg actgttgcac agcgccgcgc
     1681 cgcccagctc gcgcatcagc agccaggtat cgacataggt gcgcggcaac tggtcagggc
     1741 tgtacaggta gtcggccagc gccatttgcg cgtttgccga gaacaggtgt gcgccaagct
     1801 gcgtcttcag cgtctgcaat gcactcttgg cgattgttcc cgccggaatc gagaagcgcg
     1861 accataccag aagcggcaca ttgaacagca ggatatcgta gtcctgcccc tgcaggctca
     1921 tgacacagga ctcggcactg gactcaagaa tgtcggcaag gctgtcgtgc gccagagcat
     1981 gcgagtcaaa cagacgatcg agcgaggcat tcagatcgtc ttcggcaccg tcgtgcagca
     2041 gtttgcagac cagttccgtg agctgcttat cccagagttt ggcctccagc cggccgcccg
     2101 actctgccag tccggtcgcc agccggacca gctcattggc atcgtccgat atgcgtttgc
     2161 gtgatgaggg tcgactgcgc ttcatttcca ttgcctgtaa attccataat ccgccattgt
     2221 accaagtcgc ggatggcgca ttcggccgcg ttcagcgccc acatgagatt tcgcggtaaa
     2281 cgcgttatcc tttcggcctg ttcttcttcg tgttcggtgg taaggagcgc cattgtgtgc
     2341 ccgagctgtt accgattgcg ccaaccccaa ggaaagacaa catgctgaag gcaagcgaat
     2401 catttcggga catacgcgac gccgtacgcg acctctgtgc gcaatatccc gccgcttact
     2461 tccgcgagat cgatcgggag cgttcctatc ccgaggcctt cgtcgatgcg ctgacccggg
     2521 ccggctggct ggcggcgctg atcccgcagg aatacggcgg ctccggcctt ggcctgaccg
     2581 aggcttcggt gatccttgag gagatcaacc gttgcggcgg caactccggc gcgtgccacg
     2641 gccagatgta caacatgggc acactgctgc gccacggctc ggatgcgcaa aagcaggcgt
     2701 atctgccacg tatcgcttcg ggtgaactgc gcctgcaatc aatggccgtg accgagccga
     2761 gcaccggcac cgacaccacg cggatcaaga ctgttgccgt gaagcagggc gaccgctacg
     2821 tggtcaacgg ccagaaggtg tggatctcgc gcgtccagca ctcggatctg atgatcctgc
     2881 tggcacgcac cacgccgctt gccgaagtga gcaggaaatc tgaaggcatg tcgatcttca
     2941 tcgtcgatct gcgccaagcg atcggccagg gaatgacggt gcagccgatt ccgaacatgg
     3001 tcaatcacga gaccaacgaa ctctttttcg acaatctgga gattccggtg gagaacctga
     3061 tcggcgagga aggcaagggc ttcaggtaca ttctcgacgg actcaatgcc gagcgcgcgc
     3121 tgatcgctgc cgagtgtatt ggcgacggct actggttcat cgacaaggtc agcagctacg
     3181 tcaaggaacg cgttgtcttc gaccgaccga tcggccagaa ccagggtgtg caattcccga
     3241 tcgcggaggc gtacatcgaa gtcgaagccg ccagcctgat gcgctacgaa gcctg
//
LOCUS       DAORPM010000262         3280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9841_length_3280_cov_10.304186, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000262 DAORPM010000000
VERSION     DAORPM010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9841_length_3280_cov_10.304186"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..484
                     /locus_tag="PLD53_10785"
     CDS             8..484
                     /locus_tag="PLD53_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HRC61445.1"
                     /translation="MAADMPKRKPGLWEINMQMDGVPNMGPMQQCIDQNTDNLMQQQA
                     KKGKTDCSVMDIKPSGNKVSIHTVCKIEGSTATTDGVFEGAFDSSYKGKLKTRFNPPM
                     HGMSESNMTQEARWLGACKPGQKPGDVIMPNMGSVNINEMMKDPKMQEMMKRQQQR"
     gene            complement(524..1081)
                     /locus_tag="PLD53_10790"
     CDS             complement(524..1081)
                     /locus_tag="PLD53_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HRC61446.1"
                     /translation="MALKATIFKLDLTIADIDRNHYASYSRMIARHPSENDARMMVRL
                     LAFMLYADDELAFGKGLSTDEEPDLWLKDLTGAVELWIVVGQPDERWLRKASGRAERV
                     IVFSYGGRGAEMWWEQNRSTLEKLPRLTVFRLSLESTQALASLADRAMTLQCTIQDGE
                     LMITGAGDVVRIEPVVLLNTDKNSN"
     gene            1216..2619
                     /locus_tag="PLD53_10795"
     CDS             1216..2619
                     /locus_tag="PLD53_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRC61447.1"
                     /translation="MRSIATLLTLVTLTGALSPDAMAIGQIDPFDTRKITPPRPQLFP
                     TEDGTYTPCQAPPAGTIYSVLEVVDLALCRNPKTQEVWANARAQAAQVGVAQSAFLPN
                     LDGTLNAGRVHTRDENTNLRSASLTLSWLLYDFGARTANLENARQLLSAAASTLDSTV
                     QSIFLTALQSYYNAQAARAAVIAAKESEKASLESLTAAEVRYRVGTGTPADRLQAQTA
                     WSQATLNRIKAEGIVKIAMGTLANVMGLDASQPLKLADIPEITPNVAYERDIDALIAE
                     ARQRRPDLKAAEAQLKAAQAGVDYTRTSGLPTLSLSAGPAWIDSGNITGNESAIGLTL
                     TLPLFSGFSTNYQVRSAQAKTEVAGAQRDNVNLQIALDVWSAYQSLTTATQAIKTSTD
                     LLVSAEQSERVALGRYKAGVGNILDVLNAQSALAGARLQRIQATLDWHVFRASLAKAV
                     GLLDASLLNPAAGTTAQ"
     gene            2616..>3280
                     /locus_tag="PLD53_10800"
     CDS             2616..>3280
                     /locus_tag="PLD53_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRC61448.1"
                     /translation="MKPTLKKTLIALIALAFVGGGIFWYRSNVALAPEQRYKLQTLEK
                     GDLTQMVSANGTLNPVVLVNVGTQVSGTVNKLYVDFNDKVEAGQPLLELDSSLLAAQS
                     RQTSANVINVNASLELARANESRMKSLFEQEYVSRQELDQAVQARKSAEAQLAQARAA
                     ADKDRVNLAYTTVRSPVSGIVVNRVVDLGQTVAASLQTPTLFQIAQDLSEMRIDTSFA
                     EAD"
BASE COUNT          751 a         1048 c          898 g          583 t
ORIGIN      
        1 agcagcggtg gctgccgaca tgcccaagcg caaacctgga ctgtgggaaa tcaacatgca
       61 gatggacggc gttcccaaca tgggcccgat gcagcagtgc attgatcaga acaccgacaa
      121 tctcatgcag cagcaggcca agaaagggaa aaccgattgc agcgtgatgg atatcaagcc
      181 ctcaggcaac aaggtgtcca tacataccgt gtgcaagatt gaaggatcga ccgcgacaac
      241 ggacggcgtc ttcgagggtg ctttcgactc aagctacaaa ggaaagctga aaacgcgctt
      301 caacccgccg atgcatggca tgagcgaatc caacatgacg caggaagccc gctggctcgg
      361 tgcctgcaag ccgggtcaga aacccggtga tgtgatcatg ccgaacatgg gcagtgtgaa
      421 catcaacgaa atgatgaagg atccgaaaat gcaggaaatg atgaaacggc agcagcaacg
      481 ctgatccgag ccgaaatggc caccctaccg ggcggggtcg gtttcagttg ctgtttttgt
      541 cagtattcag gagcacaacg ggttcgatcc gaaccacgtc ccccgcgccc gtaatcataa
      601 gttcgccatc ctgaatcgtg cattgcaggg tcattgcccg atcggcgagt gaagcgagtg
      661 cctgcgtgct ttccagcgag agcctgaata cggtcagcct cgggagtttt tccagcgttg
      721 agcggttctg ttcccaccac atttccgccc cccggccacc ataggaaaac acgattaccc
      781 gctccgctcg cccggatgcc ttgcgcaacc agcgctcgtc cggctggccg acgacaatcc
      841 agagctcaac agcccccgtc aggtccttga gccaaaggtc gggctcctcg tccgttgaca
      901 agcctttgcc gaaagccagc tcatcgtccg catagagcat gaaggcgagc agacgaacca
      961 tcatgcgcgc gtcgttttcg gaaggatgac gggcgatcat gcgcgagtaa cttgcgtaat
     1021 gattgcgatc gatatccgca atcgtgagat ccagcttgaa aatcgtcgct ttgagggcca
     1081 tgggtatttc ttcgtatcct gcgtgtcacg caggcaccgt ctgaaacatt tgataactgc
     1141 tgggcagacc ggcagcaagg tggaaagaat gaaggccgct atactagctg aaaatttgat
     1201 cccctctacg aaccgatgcg ttcaattgcc acattgctga cccttgtcac cctcaccggc
     1261 gcactctcac ccgacgcgat ggccattggg cagatcgatc ctttcgacac ccggaagatc
     1321 acgccgcctc gcccacaact cttcccgacg gaagacggaa cctacacccc ctgccaggcg
     1381 ccgcccgccg gaacgatcta cagcgtactt gaagtcgtcg acctcgcctt gtgccggaac
     1441 ccgaaaactc aggaggtctg ggccaacgca cgggctcagg cggcccaggt cggtgtcgcg
     1501 caatccgctt tcctgcccaa tctggacggc acgctaaacg cgggtcgcgt gcatacccgt
     1561 gacgaaaaca ccaatctgcg cagcgcctcg ctaactcttt cgtggctgct ttacgatttt
     1621 ggcgcgcgta cggccaacct cgaaaatgcc cgacaacttc tcagtgccgc cgcctcgacg
     1681 ctggactcca cggtgcaaag catcttcctg accgcgctcc agtcctatta caacgcgcag
     1741 gctgcgcgcg ccgccgtgat tgccgcaaag gaatcggaaa aggccagcct cgaaagcctc
     1801 actgccgccg aggtgcgcta cagggtcggc accggcacgc cggccgaccg cctgcaggcg
     1861 cagacggcct ggtcgcaagc cacactgaat cgaatcaagg ccgaaggtat cgtcaagata
     1921 gcaatgggca cgctggccaa cgtgatgggc ctcgatgcca gccagccatt gaagctcgcc
     1981 gacatcccgg aaattacccc caacgtcgcg tatgagcgcg acattgacgc cctgatcgcc
     2041 gaagcacgcc agcggcgccc cgatctgaag gccgccgaag cgcaactcaa agccgcgcag
     2101 gccggcgttg actacacgcg cacttccggc ctgccaacac tatcactcag tgccgggccg
     2161 gcctggattg actccggcaa cataaccggc aacgaaagcg caatcggtct tacgctcacg
     2221 ctgccgctct tttccggctt cagtacaaac taccaggtgc gctccgcaca agccaaaacg
     2281 gaggtagctg gcgctcagcg tgacaatgtc aacttgcaga tcgcactcga cgtgtggagc
     2341 gcctaccaaa gtctgacgac agcgacgcag gcgatcaaga caagcaccga tctgctggtc
     2401 agcgccgagc agtcggaacg cgtcgcactc ggccgctaca aggccggtgt tggcaatata
     2461 ctggatgtgc tgaacgcaca gagcgccttg gcgggcgccc ggctgcaacg cattcaggcc
     2521 acgctggact ggcacgtatt tcgcgcctct ctggccaagg ccgttggcct gctcgacgcc
     2581 agcctgctga atcctgctgc gggaacaact gcccaatgaa accgacactc aagaagactc
     2641 tcatcgcact cattgccctt gcctttgtcg gtggcgggat cttctggtat cgcagcaatg
     2701 tggcactggc gcccgaacaa cgctacaagt tgcaaaccct cgaaaaaggc gatctaacgc
     2761 agatggtttc ggccaacggc accctgaatc cggttgtgct ggtcaatgtc ggcacgcagg
     2821 tttccggtac cgtcaacaag ctttacgtcg atttcaacga caaggtcgaa gcaggtcagc
     2881 cgctgctcga gctcgacagt tcactgcttg ccgcgcaatc acgtcagact tcggccaacg
     2941 tcatcaacgt caacgcctcc cttgaacttg cgcgcgccaa cgaatcgcgc atgaagtcac
     3001 tcttcgaaca ggaatacgtc tcccgccagg aactcgatca ggcggtgcag gcgcgcaaat
     3061 cggccgaagc ccaactggca caggcccgag cggccgccga caaggatcgc gtcaacctcg
     3121 cctacacaac cgtccgttcg ccggtgtcgg gcatcgtggt caaccgtgtc gtcgacctcg
     3181 gacaaaccgt tgcagccagc ctgcaaacgc caacgctctt ccagattgcc caggatctct
     3241 ccgaaatgcg catcgacacg agctttgcgg aagccgacat
//
LOCUS       DAORPM010000263         3263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9929_length_3263_cov_13.754364, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000263 DAORPM010000000
VERSION     DAORPM010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9929_length_3263_cov_13.754364"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLD53_10805"
     CDS             complement(<1..986)
                     /locus_tag="PLD53_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRC61449.1"
                     /translation="MAILACSVLPLFSGAAWADRIKDLASVQGIRNNQLVGYGLVVGL
                     DGTGDQTTQTPFTTQSIINMLAQLGTTLPTTQSLQLKNVAAVMVTANLPAFARIGQQI
                     DITVSSMGNAKSLRGGTLVMTPLKGADGQIYAQAQGNLLVGGAGAVASGSKAVVNHLL
                     AGRIVAGATVEREVPTALGQGPFVHYEMRTTDFGTTQRVVEMINREMGPGTAQAVDGR
                     LIQVIAPEGSSARVAFMGRIENLEVRPVKAMARVVVNPRNGSVVMNQMVTIESCAVAH
                     GSLSVVVKTAQEVSQPNAFADGQTVTTSQSDIEIKQGGGALMHLKAGVNLSDV"
     gene            complement(1033..1698)
                     /locus_tag="PLD53_10810"
     CDS             complement(1033..1698)
                     /locus_tag="PLD53_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRC61450.1"
                     /translation="MMNNRFLLILLGVVIGGCTTVPPTNVHQPMTARPAPRTETLPAS
                     GSIYQAGASRTLFEDRRARYVGDTMTIAIAETTSASTKSNTNASRSTSIDASVPLITG
                     LPGKSLQGLGLSATGSNSLAGKGDAAANNVFTGTITVTVIEVLSNGNLLVSGEKQVSI
                     GAGTEYIRLSGIVNPYFINAANTISSGNVADARIEYKESGVISEAQVMGWLARFFLSV
                     LPF"
     gene            complement(1706..2488)
                     /gene="flgG"
                     /locus_tag="PLD53_10815"
     CDS             complement(1706..2488)
                     /gene="flgG"
                     /locus_tag="PLD53_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRC61451.1"
                     /translation="MIRTLWIARTGINAQQVSLEVIANNLANLSTPGFKRQRPVFEDL
                     LYQTLRQPGAQSSQSTQIPSGLQIGTGVSPVATATIFTQGALQQTGNALDVAIDGQGF
                     LQILLPDGTTAYTRDGSFQKDNQGQLVTSSGYPVQPSITIPSDALTVTIANDGVVSVT
                     QPGNSSATQVGSLQLATFINNGGLQRIGENLYLETASSGSPTPNTPGTNGSGVLLQTY
                     VETANVNVAEELVSMIQTQRAYEMNTKAVRTADEMLARLGQL"
     gene            complement(2503..3246)
                     /locus_tag="PLD53_10820"
     CDS             complement(2503..3246)
                     /locus_tag="PLD53_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HRC61452.1"
                     /translation="MDRLIYTAMTGAKHAFLQQAGVAQNLSNASTVGYRAMEHKFRAV
                     PVQGDGAPTRAFVVDASVADVFEQGPLMATGRPLDVAVQGAGWITVETPDGREVYTRA
                     GNLQVNTNGQLQTATGLNVLGDNGPIAIPPDNSITIAPDGTISTVPLFGAPNNVSVVG
                     RIKLVNPAENDLVRGDDGLFRTRVGEPAVLDENVKLAPETLEGSNVNAADSLVNMITL
                     ARQFDMQIKMLQTADANANKASQILSMNA"
BASE COUNT          676 a          963 c          886 g          738 t
ORIGIN      
        1 acatccgaaa gattcactcc agctttcaaa tgcatcagcg ccccgccacc ctgcttgatt
       61 tcgatgtcgg actgcgaggt tgtcaccgtc tgcccatcgg caaacgcgtt tggctgacta
      121 acctcctgcg cggttttcac taccactgaa agcgagccat gcgcgacagc gcaggactcg
      181 atggtgacca tctgattcat cacgacagac ccgttgcgag gattcacgac aaccctggcc
      241 attgctttca ccggacgaac ttcaagattc tcgattcggc ccatgaaagc cacccgggca
      301 ctggatccct ctggggcaat aacctgaatc agccgcccat caaccgcttg cgccgtcccg
      361 ggccccattt cacggttgat catctcgacc acgcgttgcg tggtgccgaa gtcggtagtt
      421 ctcatttcat aatgaacaaa aggaccctgc cccagcgctg tcggcacctc tcgctccacc
      481 gtggcacccg caacaattcg acccgccaga agatggttaa ccacggcttt gctcccgctg
      541 gctactgctc cagcgcctcc aaccaggagg ttgccttgcg cctgggcata aatctggcca
      601 tcggcgccct tcaatggggt catcaccaag gtgccgccgc gcaggctttt ggcattgccc
      661 attgacgata cggttatatc gatttgctga ccgattctcg cgaatgctgg cagattcgcc
      721 gtgaccatca ccgccgcaac gtttttcagt tgcagggatt gagtcgtcgg caacgtggtg
      781 cccaattgag ccagcatatt gataatgctt tgtgtggtaa acggcgtctg tgtcgtctgg
      841 tcgcctgtcc cgtcgagtcc gacaaccagc ccatagccaa ccagctggtt gttccgaatc
      901 ccctgaaccg aggccaggtc cttgattcga tccgcccatg cggcaccact gaagagcggc
      961 aagacgctac aggcaagaat tgccacaaag tgcaggaatt tgccggtctt attgatcttc
     1021 acgactgact cctcagaagg gcaaaacaga aaggaagaat cgtgcgagcc agcccatcac
     1081 ctgcgcttcg ctgatcaccc cactctcctt gtattcgatt cgcgcatcag ccacattccc
     1141 tgagctgatc gtattcgccg cattgatgaa atagggattc acgatcccgg aaagccggat
     1201 gtattcggtg ccagccccga tggacacctg cttttccccg gacacaagaa ggttgccgtt
     1261 ggacagcacc tcgatcacgg taaccgtgat cgtccccgta aagacattgt tcgctgctgc
     1321 atcgcctttt ccagccagac tgtttgagcc ggttgccgac aaacccagcc cttggagtga
     1381 cttgcccggc aagccggtga tcagcggtac cgaggcgtcg atgctggtgg aacgactggc
     1441 attggtattc gattttgttg aggccgacgt cgtttcggca atcgcaatcg tcatcgtgtc
     1501 gccaacgtag cgggcacggc gatcctcgaa cagcgttcgt gaagcgccgg cctggtaaat
     1561 actgcctgat gccgggagcg tttcggttcg cggcgccggc cgcgcagtca tcggctgatg
     1621 cacattggtt ggcggtacgg ttgtacatcc accgatgact accccaagaa gaatcaataa
     1681 gaaccggtta ttcatcatcg ctgcattaca gctgacccag tcgcgcgagc atttcgtccg
     1741 ctgttctcac cgccttggta ttcatttcat aagcgcgttg ggtctggatc atactcacca
     1801 gttcctcggc aacgttgacg ttcgccgtct cgacataagt ttgcaggaga acccccgaac
     1861 cattggtgcc aggcgtgttc ggagtcggcg atccactcga cgccgtttcc agatagaggt
     1921 tttcaccaat tcgctgcaag cccccgttat tgataaaggt cgcgagctgc aatgagccaa
     1981 cttgcgtagc agaagaattc cccggttgcg taacggaaac cacgccatcg ttggcgatcg
     2041 tgacggtcag cgcgtcggac ggaatggtga tactcggctg gacaggatat ccgcttgagg
     2101 ttaccaactg gccctgatta tctttctgaa acgagccatc tcgggtatag gccgtcgttc
     2161 catcgggcaa caggatctgc aggaaaccct gcccatcaat ggccacatcg agcgcgttgc
     2221 ctgtttgctg caaagcgccc tgtgtgaaaa tggttgcagt cgctacgggc gaaacaccgg
     2281 taccgatttg caggccggat ggaatctggg tcgactgcga cgactgagcc ccaggctggc
     2341 gcaaggtttg ataaagcaga tcctcaaata cgggacgctg ccgtttgaag ccaggggtgc
     2401 tgaggttggc cagattattg gcgatcacct ccaggctgac ttgctgggca ttgatgccgg
     2461 tacgggcaat ccacaaagta cggatcatgc gtgctaatcc tgttaggcgt tcatcgaaag
     2521 aatctggctt gccttgttgg cattggcatc agctgtctgc agcatcttga tctgcatatc
     2581 gaactgacga gccagggtga tcatgttgac cagtgagtcg gctgcgttca cattgctgcc
     2641 ttccagtgtt tcaggagcca gtttgacgtt ttcatctagc acagccggct cgccgaccct
     2701 ggttcggaaa agtccgtcat ctccgcgtac cagatcgttt tcagccgggt ttaccagctt
     2761 gatgcgcccg acaacactga cgttatttgg tgcgccaaac agcggaacgg tggaaatcgt
     2821 tccgtccgga gcgatcgtga tgctgttgtc cgggggaatt gcgatcgggc cgttgtcgcc
     2881 cagcacgttc aatccagtgg cggtctgaag ttgaccattg gtgttgactt gaaggtttcc
     2941 ggcccgggtg tacacctcgc gcccatcggg agtttccacc gtaatccagc ctgccccctg
     3001 aacggccaca tcaagcggac ggcctgtcgc catcagcgga ccttgttcaa acacatcggc
     3061 caccgacgcg tccaccacaa atgcccgtgt tggggcgccg tcaccttgaa caggtaccgc
     3121 gcgaaacttg tgctccatgg cgcgataacc aaccgtggaa gcattggaca aattttgcgc
     3181 gactccagcc tgttgcagga aggcgtgctt ggcgccggtc attgcggtgt agatcaggcg
     3241 atccataacg gcctcggttt tgc
//
LOCUS       DAORPM010000264         3255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_9966_length_3255_cov_12.255625, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000264 DAORPM010000000
VERSION     DAORPM010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9966_length_3255_cov_12.255625"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLD53_10825"
     CDS             <1..471
                     /locus_tag="PLD53_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRC61453.1"
                     /translation="TSSATLLAPSVAFSENILRNYFPDITDHSFLRMLRITIVCFACI
                     VLAFALGSDSTIFKMVENAYKVTLSGAFAPLFFGAYWRRSTTQGALFSIIGGVSSWLL
                     LELLIGEASVIPPQLIGLGVGIISMIAGSLLPQWIGLPDVKGDERAEQLRARNQ"
     gene            554..1696
                     /locus_tag="PLD53_10830"
     CDS             554..1696
                     /locus_tag="PLD53_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRC61454.1"
                     /translation="MNPLTLTVVAMPSSPKSIAGSIAGKELAPLPTLLPHYLALSYAA
                     LIIYASLHPLTGWRDPGISPFYFLDAAWPRYWTGFDLAINALAYLPLGFLLALTLRRL
                     PGRWIPAVTALMLGTLLSFCVESLQTWLPTRVASNLDLACNTLGTGIGALLSIWHGRR
                     FFIRIALLQRHLLTPFPHSDFGLVLIGLWLLTQLSPETILFGAGDMRHMLEITPAVPY
                     AAHSFFALETGIIVCNTIAIGLTVRALLADQSSPYLAVAVFFVVALSIRTLAAAVLVN
                     PLNAFAWLTPGATLGLSVGGVILSLIMLMPASIRIALAGLALMAGTVLVNVTPPNPYS
                     AAALVTWRQGHFLNFNGLTRLTASFWPFLALPYLTLLGRRIESNQG"
     gene            1744..2052
                     /locus_tag="PLD53_10835"
     CDS             1744..2052
                     /locus_tag="PLD53_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRC61455.1"
                     /translation="MSYYKHHVFFCCNQRAEGESCCNNLGATEAQTYAKDRIGQLKLK
                     GKGKIRVNKAGCMDRCDEGPIIVVYPEEIWYHYVDKEDIEEIIQEHLVQGRVVERLKI
                     "
     gene            2070..2708
                     /locus_tag="PLD53_10840"
     CDS             2070..2708
                     /locus_tag="PLD53_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRC61456.1"
                     /translation="MKLNEERVLIDGPAGAIELIVENPGAPRGIALICHPHPLFGGAN
                     TNKVTQTLARTFTHLDYVALRPNFRGVGGSGGTHDNGNGECEDMLTILAEAKRRYGDL
                     PVALAGFSFGAYVQTRVAQALIEANHPAQRLVLVGTAAGHVEGARQYTVKPVANDTIV
                     IHGSVDTTVPLANVLAWAEAMNLPVIVVPGADHFFHRRLHVIREIITRAWRH"
     gene            2696..>3255
                     /locus_tag="PLD53_10845"
     CDS             2696..>3255
                     /locus_tag="PLD53_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRC61457.1"
                     /translation="MASLNAADALCVSSLSKRYDSHEVVAGISFTVPRGTCFGLLGPN
                     GAGKTTTLRCCLGLTTADAGRISLGGHAIPEQAGAARERVGVVPQLDNLDPDFTVWEN
                     LLVYGRYFGIDDALIGERIPDLLDFAGLGGKRAARIQTLSGGMKRRLTLARALVNDPD
                     IVFLDEPTTGLDPQARHLIWDRLKQLT"
BASE COUNT          636 a         1017 c          878 g          724 t
ORIGIN      
        1 acctcgtcgg caacgctact cgcgccatcg gttgctttct cggaaaacat cctgcgcaac
       61 tatttccctg atatcacgga tcattctttt ctgcgcatgc tgcgcattac cattgtttgc
      121 tttgcctgca ttgtgctcgc ctttgccctc ggctctgact ccaccatctt caaaatggtc
      181 gagaacgctt acaaggtcac gctttccggc gctttcgcgc cactcttctt cggcgcctat
      241 tggcggcgca gcacgacgca gggcgcactg ttttcgataa ttggcggagt gtcgtcctgg
      301 ctccttctcg aactgttgat cggcgaagcc agtgtaatac cgccgcaact catcgggctg
      361 ggtgtaggga ttatcagtat gatcgccggc tccctactgc cacagtggat tggtctgccc
      421 gatgtgaagg gcgatgagcg cgcggaacaa ctacgcgcgc gaaatcaata aaccccccgg
      481 agtcgctaca gccgtttctc ggcatccgcc acggattcac gaatccgatg cgtgtcaatc
      541 gtgaagcgtt agaatgaacc cactaacgct cacggtcgtc gcgatgcctt catcacccaa
      601 atcgatagcg ggaagtatcg ccggcaaaga actggcgcca ttgcccacgc tgctaccgca
      661 ctatctggca ttgtcctacg cggcgcttat catttatgcc agtctgcacc ctctgaccgg
      721 ttggcgcgat ccgggaatat cgccattcta ttttctcgac gccgcctggc cacgctactg
      781 gacgggtttc gatctcgcga tcaacgctct cgcttatctg ccgctgggtt ttctactggc
      841 actgacactt cgccgcttac ccggtcggtg gattccagca gtcaccgcat tgatgctggg
      901 taccctgctc agtttttgcg ttgagagcct gcaaacatgg ttgccaacgc gtgtcgcctc
      961 aaacctggac ctggcctgca atacgttggg aacgggcatc ggcgccctgc tttcgatctg
     1021 gcatggcagg cgcttcttca tccgtattgc gctgcttcag cgccatttgc tgacaccttt
     1081 tccccattcc gacttcggac tggtcttgat cggtctttgg ctgctgacgc aactctcgcc
     1141 ggaaacaatc ctcttcggtg ccggcgatat gcgacacatg cttgagatca ccccggccgt
     1201 accctatgca gctcactcgt tctttgcact ggagaccggc atcatcgtct gcaacacgat
     1261 cgctatcggc ctgactgtcc gcgccctgct tgccgaccaa agctcaccct acctggcggt
     1321 ggccgttttc ttcgttgtcg cgctctcgat ccggacgctg gccgcggcgg ttctggtcaa
     1381 ccctctcaat gcctttgcct ggcttacccc cggcgccacg cttggcttgt ctgtcggggg
     1441 cgtgattctt tcgctcatca tgttgatgcc cgcatcgata agaatagcgc tggccggact
     1501 ggcgctcatg gccggaacgg tactggtcaa cgtcacccca ccgaatccct actcggctgc
     1561 tgcgctggtc acctggaggc aggggcattt ccttaatttc aacggattga cccggctgac
     1621 agcaagcttc tggccattcc ttgccctccc ctatctgacc ttgctcggaa gacgtatcga
     1681 atcgaatcaa ggttagaatc ggccatcgca ggaagcacaa tcatcaatga ggcatttgtc
     1741 agaatgagct actacaagca tcacgttttc ttttgctgca accagcgcgc cgaaggcgag
     1801 tcatgctgta acaacctggg cgccaccgag gcacaaacct acgccaagga tcgcatcggc
     1861 caattgaagc taaaaggcaa gggcaagata cgcgtcaaca aggccggctg catggatcgc
     1921 tgcgacgaag gcccgatcat cgtcgtctat cccgaggaaa tctggtacca ctacgtcgac
     1981 aaggaagaca tcgaagaaat cattcaggag catctggtcc agggacgcgt cgttgaacgt
     2041 ctgaagattt gaccagggga agaaccttca tgaagttgaa cgaagagagg gtactcatcg
     2101 atggcccggc cggcgcgatc gagctgattg tcgagaatcc gggcgcacca cgtggcattg
     2161 ctctgatctg tcatccacac ccgctgtttg gcggagccaa taccaacaag gtgacacaaa
     2221 cgctggcacg cacgtttacg catctggact atgtagcctt gcgaccgaat tttcgcggcg
     2281 tcggtggaag tggcggcacg cacgataacg gaaatggcga atgtgaggac atgctcacga
     2341 ttctggcgga agccaagcgc cgttatggcg atctgccggt cgcgctggcc ggcttttcgt
     2401 ttggcgccta tgtgcagacc cgtgtcgcgc aggcgcttat cgaggccaat cacccggccc
     2461 aacggctggt tctggtcggc acggccgccg gccatgttga aggcgcacgt cagtacaccg
     2521 tcaagcccgt tgccaacgac accatcgtca tccacggctc ggtagacacg accgttccgc
     2581 tggccaatgt attggcctgg gctgaagcca tgaatctgcc cgtgatcgtt gttccaggcg
     2641 ccgaccactt ctttcaccgt cggctgcatg tcattcgcga aatcattacc cgagcatggc
     2701 gtcactgaac gctgctgacg cgctgtgcgt ctccagcttg agcaagcgct acgatagcca
     2761 tgaagtggta gccggaatca gcttcaccgt acctcgcggc acttgtttcg gcctgctcgg
     2821 gccgaacggt gccgggaaaa ccaccaccct tcgctgctgc ctcggactga cgacggcaga
     2881 cgccggccgc ataagccttg gtggccacgc catcccggag caagccgggg cggcacgcga
     2941 acgtgtcggc gttgtacctc aactcgataa tcttgaccct gacttcactg tctgggaaaa
     3001 cctgctggtc tatggtcgct acttcggcat cgacgatgcc ctcattggcg aacgcattcc
     3061 tgatctgctg gatttcgcgg gactcggagg gaagagagcc gcccgcatcc agacactatc
     3121 cggaggtatg aaacggcgcc tgaccctggc ccgcgcgctg gtcaacgacc cggacatcgt
     3181 cttcctcgac gaaccgacca ccggcctcga tccacaagcg cgccacctga tctgggatcg
     3241 tctgaagcaa ctcac
//
LOCUS       DAORPM010000265         3233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10077_length_3233_cov_7.608874, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000265 DAORPM010000000
VERSION     DAORPM010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10077_length_3233_cov_7.608874"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PLD53_10850"
     CDS             <1..1322
                     /locus_tag="PLD53_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRC61458.1"
                     /translation="PDLHSGRRRPVNHRPLREHASMLIASAFILLVFAYSLVSRKLES
                     TLITAPILFTAAGAFMLLFPEASRELFIDRKALLVIAEVGLVMTLFTDASHINRRALQ
                     DNHKLPVRLLSSGMLLTILLGAAAAMACFAGLSWWEAGILAAILAPTDAGLGQIIVNS
                     PRVPARIRQALNVEAGLNDGLAVPFLMLFIALATAIEDGASTRGVLARFLLEQIGYGS
                     VIGLVIGLGGGLLMGLAQRRQWMSPSLGQLGVVAMPLLCIMAAEESHASMFIAAFVAG
                     LAAQVGFPKIGRHCVEFTEGWGQLFDFFVFFLFGLLFARAWHDFTPAILLYALLSLTV
                     VRMLPVAVAVAGIGLSRTTVLFLGWFGPRGLASIVLGLVYLEQQENLPGEDTIRLAVM
                     ATVLLSIVAHGLSAKPGIERYARSCAALPASAPEHAAVNTADDFGEK"
     gene            1511..1876
                     /locus_tag="PLD53_10855"
     CDS             1511..1876
                     /locus_tag="PLD53_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HRC61459.1"
                     /translation="MNFDPYNSELCLAHNAPVRLMSARGVRVICTAGRIWLTVEGEAG
                     DVFLAAGESHLIRGRGLALLEAIGSGRVRFQKAARPFRSLIQALSLSRLKGSSRLGVS
                     CHDASGSRMTLRLTPAADL"
     gene            1955..2899
                     /locus_tag="PLD53_10860"
     CDS             1955..2899
                     /locus_tag="PLD53_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRC61460.1"
                     /translation="MQFKDYYQTLGVERSATQDDIKHAYRRLARKYHPDVSKEADAET
                     RFKDIGEAYAVLKDPEKRVAYDQMGSSWKAGQDFQPPPGWDAGFEFSGREAGPGDAAE
                     FSDFFETLFGRQGWGAQPHRASMHAQGQDHHAKVMIDLHDAYRGAKRSVSLRMPVLDA
                     QGRVATHERTLDVSIPKGVRAGQHLRLAGQGGPGLGEGPAGDLYLEIDFNPHSNFRAD
                     GRDVYLDLPVAPWEAALGASVSTPTPEGPVQLTIPPDSAAGRQLRLKGRGIPGTPPGD
                     LYVVLKIALPPADSEVKREAYRAMAQAFDFNPRTHLKG"
     gene            2906..3220
                     /locus_tag="PLD53_10865"
     CDS             2906..3220
                     /locus_tag="PLD53_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HRC61461.1"
                     /translation="MNQANVTFIQGTVVEEVHLTLVELCQACCADETHIHAWVLEGVL
                     EPLGNSPQDWRFSGDSLRRAQLALRLSRDLDINPPGVALALDLLDRIAALETRLQHTG
                     NH"
BASE COUNT          588 a          975 c         1000 g          670 t
ORIGIN      
        1 atccagatct gcattccggg cgtcggcgcc ccgtcaatca tcgtccgctt cgggaacatg
       61 cttccatgtt gatcgcttct gcattcattc tccttgtctt cgcctacagc ctggtgtcgc
      121 gcaaactgga aagcacgctc atcaccgcgc cgatcctgtt cactgcagcg ggcgcgttca
      181 tgctgctctt tcccgaggcg tccagggaac tgttcatcga ccgcaaagcg ctcttggtaa
      241 tcgccgaagt cggtctggta atgaccttgt tcaccgacgc gtcgcacatc aaccgacgcg
      301 ctctgcagga caaccacaag ttgccggtgc gtttgctcag ctccggcatg ctgctcacca
      361 tcctgctcgg tgcggcagcg gcgatggcgt gcttcgctgg gctgtcgtgg tgggaagcgg
      421 gtattctcgc ggcgatactc gcaccgaccg acgccggcct cggtcagatc atcgtcaata
      481 gcccgcgcgt cccggcgcgc atacggcagg cactgaacgt cgaggccgga ctcaacgacg
      541 ggttggcggt ccctttcctg atgctgttca tcgcactcgc caccgcgatc gaagatggag
      601 cgagcacacg gggcgtactc gcgcgttttc tcctcgaaca gatcggctac ggcagcgtga
      661 tcgggctggt gatcgggctg ggcggcggtc tgctgatggg gctggcgcag cgccggcagt
      721 ggatgtcccc ctcgcttggc caacttggcg tggtggcgat gccgctgctg tgcataatgg
      781 cggcggaaga atcccacgcg agcatgttca tcgccgcatt cgttgccggt ctggcggcgc
      841 aggtgggatt ccccaaaatc ggacgccact gcgtcgagtt caccgaaggt tggggacaac
      901 tgttcgattt tttcgtcttc ttcttgttcg ggctgctctt cgcgcgcgcc tggcatgatt
      961 tcacaccggc catcctcctt tatgcgcttc tcagcctgac ggtcgtgcgc atgctgcctg
     1021 tcgcagtggc ggttgccggt atcggactca gccgaacgac cgtacttttc ctcggctggt
     1081 tcggcccgcg cggcctggcg tcgatcgttc tggggctggt gtatctggag caacaggaga
     1141 atttgccggg cgaggacacc atccgtctgg cggtgatggc taccgtgctg ctcagcatcg
     1201 tcgcacatgg cctgagtgcc aagcccggca tcgagcgcta cgcccgcagc tgcgcagcac
     1261 tgccagcaag tgccccggaa catgcggccg tcaacacggc ggatgatttc ggcgagaaat
     1321 aggccctcgt ttaaccctcg tctcccatcg gattcaccaa cacagttgcg gcttatcacg
     1381 acaggtacaa ttgcaccgac ttagaactgt accggtaaca attcgctata tttgtggctg
     1441 tttctcctga acctggcgtt ctacacttcc cttcaccgaa gcaaggcctg ccgcacaagg
     1501 agagccgctt atgaatttcg atccgtacaa cagcgaattg tgtctcgccc acaacgcgcc
     1561 agtgcgcctg atgtcggccc gcggcgtacg cgtcatttgc actgcaggca ggatctggtt
     1621 gaccgtcgag ggcgaagcgg gcgatgtatt ccttgccgcc ggcgaaagcc atctcattcg
     1681 cggccgcggc ctggccttgc tggaagccat cgggagcggg cgcgtcaggt tccagaaagc
     1741 cgccaggccg ttccgtagcc tgattcaggc cctgtccctt tctcgattga aaggttccag
     1801 caggctgggc gtgagctgcc acgatgcgag cggcagtcga atgactctaa ggcttactcc
     1861 tgccgccgac ctgtgaccgt tttgtccggg attcataatt tccatgaatg agtgtaagtt
     1921 gtgcacttaa taacaacatc agcgcagccc gaccatgcaa ttcaaggact actaccagac
     1981 acttggcgtt gaacgcagtg cgacgcaaga cgacatcaag cacgcctatc gcagactggc
     2041 gcgcaagtat cacccggacg tgagtaagga ggcggatgcc gagacgcgct tcaaggatat
     2101 tggcgaagcc tatgccgtat tgaaggatcc agagaagcgc gtggcttacg accagatggg
     2161 cagtagctgg aaggcggggc aggacttcca gccgccgccg ggttgggatg ccggtttcga
     2221 gttcagtggt cgcgaggccg ggccgggtga tgcggctgag ttcagcgatt tcttcgaaac
     2281 gcttttcgga cgacagggct ggggggcgca accgcatcga gcgtccatgc atgcccaggg
     2341 gcaggatcac catgccaagg tgatgatcga cctgcacgat gcctatcgcg gagcaaagcg
     2401 cagcgtttcg ctgcgcatgc cagtgctcga tgcgcagggc cgggtggcaa cgcatgagcg
     2461 cacgctggac gtgagcatcc ccaagggcgt tcgtgctggc cagcacttgc gtctggctgg
     2521 ccagggcggt ccggggctgg gtgaggggcc ggcaggcgac ctgtatctgg aaatagattt
     2581 caacccgcat tcgaattttc gggccgacgg tcgcgacgtc tatctggacc tcccggtggc
     2641 accttgggaa gctgcccttg gcgcctcggt cagcacgcca acgccggagg ggccggtgca
     2701 gctcacgatc ccgcccgact cggcagcagg tcgtcagttg cgcttgaaag gccgcggcat
     2761 tccgggaacg ccaccgggcg atctttacgt cgtcttgaaa atcgccctgc cgccggccga
     2821 tagtgaagtc aagcgcgaag cctaccgggc aatggcgcag gcctttgatt tcaatccccg
     2881 aacacacttg aaaggataag cggtcatgaa ccaagccaat gtgacattta tccagggaac
     2941 ggtggtggag gaagtccatt tgacgctggt ggaactgtgt caggcctgtt gcgccgacga
     3001 aacgcacata cacgcctggg tattggaggg tgtgctggaa ccgctgggaa attcgcccca
     3061 ggattggcgc ttcagcggcg actccttgcg ccgcgcgcag ctggccctgc ggctgtcgcg
     3121 cgatctggat atcaatcctc ccggcgtcgc tttggcactc gatttgctcg atcggattgc
     3181 cgctttagag actcgcctgc aacacacagg gaaccattga ttcggctgaa tcc
//
LOCUS       DAORPM010000266         3204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10232_length_3204_cov_12.469355, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000266 DAORPM010000000
VERSION     DAORPM010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10232_length_3204_cov_12.469355"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLD53_10870"
     CDS             complement(<1..618)
                     /locus_tag="PLD53_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC61462.1"
                     /translation="MELAKSFEPADIERRWYPDWEAKGYFNAGLDTSNQNAFCILLPP
                     PNVTGTLHMGHGFNQAIMDALTRYHRMRGDNTLWQPGTDHAGIATQIVVERQLDAQGI
                     SRHDLGREKFLEKVWEWKEFSGNTITQQMRRLGTSPDWTRERFTMDAGLSKIVTETFV
                     RLHKERLIYRGKRLVNWDPVLHTAVSDLEVVQEEEDGFMWHIRYPL"
     gene            complement(720..1142)
                     /locus_tag="PLD53_10875"
     CDS             complement(720..1142)
                     /locus_tag="PLD53_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61463.1"
                     /translation="MTEHSDVVDRADSLMRRRRSFVASVSGKTEPISAPPPPISDDED
                     LPVLTEVVSAEAIIPETQPEPYDEEALLAIVAADLVRTLERQLAIELPSLIEATLLNA
                     QEELRSGINSTLDMALRDFLARRHQLSLSLNEPSRDDD"
     gene            complement(1142..1567)
                     /locus_tag="PLD53_10880"
     CDS             complement(1142..1567)
                     /locus_tag="PLD53_10880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HRC61464.1"
                     /translation="MTQVFFYHNVADRIAAVVALIGKAFLQRKALLVYAPDAEVAGML
                     DRQLWTHPPTGFIPHVRGDSPLAAETPVLITNDLESMLHNERLFNLSSEIPPGFSRFN
                     SVIEVVGQHESERLAGRDRVKFYKDRGYEIKYFNLSEKH"
     gene            complement(1564..3084)
                     /locus_tag="PLD53_10885"
     CDS             complement(1564..3084)
                     /locus_tag="PLD53_10885"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRC61465.1"
                     /translation="MEFSIKRGSPEKQHCACVVVGVFEPRKLTRSAVSIDNAARKHLS
                     DIVQRGDMNGKAGSTLLLHNVPSTACDRVLLVGLGKEKEFHEKEFCNAIRSAVKALHE
                     TGATDGTVFLTEIPVRKRSVAWRIRQATIIAQDTVYRFNQFKSNKNEENRPLQKVSFT
                     IERQDELSLANEALAQGLAVAEGVTLARNLGNLPGNVCTPAHLADTALQMAKDHGLDC
                     QILERTDMETLGMHALLSVAKGSHQPPKMIVLQYKGTKAGKGSKKAADEKPLVLIGKG
                     ITFDSGGISLKPGLEMDEMKFDMCGAASVLGTIKAVAQMKLPINLTVIVPTSENMPGG
                     SASKPGDIVTSMSGQTIEILNTDAEGRLILCDAMTYAERFKPEAVIDVATLTGACVIA
                     LGHVATGLFANDDSLAHKLIQAGDEAQDRAWQMPLWDDYQDLLKSPFADMANIGGRAA
                     GSISAACFLSRYTKKFSWAHLDIAGTAWKSGADKGATGRPVPLLAHYLLKRAGKLS"
BASE COUNT          610 a          868 c          974 g          752 t
ORIGIN      
        1 cagcgggtag cggatatgcc acatgaagcc gtcttcttct tcctgaacga cttcgagatc
       61 ggagacggcg gtgtgtagca ccgggtccca gttcaccagt cgtttgccgc gatagatcag
      121 tctttccttg tgcaggcgga cgaacgtttc ggtgacgatt ttcgacagcc cggcatccat
      181 cgtgaagcgt tcgcgcgtcc agtccggcga agtgccgagg cgacgcatct gttgcgtaat
      241 ggtgttgccc gagaattctt tccactccca gactttttcc aggaactttt cacggccaag
      301 gtcatgacgc gaaataccct gcgcgtcgag ctggcgttcg acgacgatct gcgtcgcgat
      361 gccggcgtgg tcggtgcccg gttgccacag cgtgttgtcg ccgcgcatgc ggtggtagcg
      421 cgtcagcgca tccatgatcg cctggttgaa gccgtggccc atgtgcagcg tgccggtgac
      481 gttgggcggt ggcagaagga tgcagaaagc attctgattg ctggtgtcga ggccggcgtt
      541 gaagtagccc ttggcctccc agtcggggta ccagcgacgc tcgatgtcgg ccggctcaaa
      601 gcttttggca agttccatga tggacgggat gtgggtatga gaatggagga aagccggcga
      661 ttataccgga tcgaaccatt gcgctggggc gcgcctcgcg acgcgtgggg aggcgtgagc
      721 tagtcgtcgt cccgactggg ttcgttaagc gacaggctga gttgatgacg gcgcgccaga
      781 aagtcacgca gtgccatatc cagggtggaa ttgatccctg agcgcagctc ttcctgagcg
      841 ttgagcaagg tcgcttcgat caggctgggt aactcgattg ccaattgtcg ttcaagggtt
      901 cgtaccaggt cggcggcgac aatcgcgagc aaagcttctt cgtcataggg ttcaggttgg
      961 gtttccggaa tgatggcttc agcggacaca acttcggtga gcacgggcag gtcttcgtcg
     1021 tcgctgatcg gcggcggggg agcgctgatg ggctctgtct tgcccgacac tgaggctaca
     1081 aagctgcgcc gacgtctcat cagcgagtcg gcgcgatcaa caacatcgga atgctctgtc
     1141 atcagtgttt ttccgaaagg ttgaaatact tgatttcgta tccgcgatcc ttgtagaact
     1201 tcacccgatc gcggcctgca agtcgctctg attcatgttg cccgacgact tctatgacgc
     1261 tgttgaagcg tgaaaaaccg ggcgggattt cggaggaaag gttgaacaga cgctcgttgt
     1321 gcagcatcga ctccaggtcg ttggttatca gcacgggtgt ttcagcagcc agcggcgagt
     1381 cgccgcgcac atggggaatg aatcccgttg gcggatgcgt ccacaactgg cgatcaagca
     1441 tgccggcaac ttcggcgtcc ggtgcgtaga ccagcagcgc cttcctctgt aggaatgcct
     1501 tgccgatcag cgcaacaaca gcggcgatac gatcagccac attgtgatag aaaaagactt
     1561 gggtcaactg agcttcccag cacgttttaa caggtagtgt gcgagcaagg gtaccgggcg
     1621 acctgtggca cccttgtctg cgcctgattt ccaggcggtt ccggcaatgt cgaggtgcgc
     1681 ccaggagaat ttcttggtgt agcgcgagag gaaacaggcg gcagaaatgc tgcctgccgc
     1741 ccgcccgccg atattggcca tgtcggcgaa cgggctcttc aggagatcct gatagtcgtc
     1801 ccacagcggc atttgccagg cacgatcctg tgcttcgtcg ccagcttgaa tgagtttgtg
     1861 ggcgagactg tcgtcgttgg cgaaaaggcc cgtggcgacg tggccgagag cgattacgca
     1921 ggcgccagtg agcgtggcga cgtcaatcac cgcttccggt ttgaagcgct cggcgtaggt
     1981 catcgcatca cataagatca ggcgcccttc ggcgtcggtg ttgaggattt cgattgtttg
     2041 tccggacatt gaggtgacga tgtctcccgg cttgctggcc gaaccaccgg gcatgttttc
     2101 gctggtggga acgataaccg tgaggtttat cggcagcttc atttgagcga ctgctttgat
     2161 cgtgccgagc acgctggccg cgccgcacat gtcgaacttc atttcgtcca tttccagacc
     2221 gggtttgagc gaaatgccac cggagtcgaa ggtgatccct ttgccgatca gaaccaatgg
     2281 cttctcgtct gcggctttct tgctgccttt cccggctttt gtgcctttgt actgcaagac
     2341 gatcattttc ggaggttggt gcgatccctt ggctaccgag aggagtgcgt gcatgccaag
     2401 ggtttccatg tcggtgcgct caagtatctg gcaatccagg ccatgatcct tggccatctg
     2461 cagagccgta tcggccagat gcgcaggggt gcacacgttg ccgggcaggt tgccaagatt
     2521 ccgggcaagc gtgacgcctt cggcgacagc cagaccctga gccagggcct cgttggccag
     2581 actcagttca tcttgccgct caatggtaaa gctcactttt tgtagcgggc ggttttcctc
     2641 gttcttgttg ctcttaaact ggttaaagcg atagacggtg tcctgagcga tgatcgttgc
     2701 ctgccggatg cgccacgcta cactgcgttt tctgaccgga atttccgtca gaaaaaccgt
     2761 gccatcggtg gcgcctgttt cgtgaagtgc tttgaccgcg ctacgaattg cgttgcagaa
     2821 ttccttctcg tgaaattcct tttccttgcc cagcccgacc aataggacac gatcgcacgc
     2881 ggttgacggc acattgtgca gaagcaatgt tgatcccgct tttccgttca tgtcgccacg
     2941 ctggacgatg tcggaaagat gcttgcgtgc cgcattgtcg atcgagacgg cggagcgtgt
     3001 cagtttgcgt ggttcgaaaa cgccaacgac gacacatgca cagtgttgct tctccgggct
     3061 tccccttttt atgctaaatt ccaacggcta ctcctcggtc ggtacagatg cgtcctaaac
     3121 gtcgattatc acagcagttt ttcgttaaag tcaaaaaatg atcttccaga atgccacccg
     3181 gcgcgagttt acccacgcgg ctgc
//
LOCUS       DAORPM010000267         3196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10273_length_3196_cov_15.939510, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000267 DAORPM010000000
VERSION     DAORPM010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10273_length_3196_cov_15.939510"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..429)
                     /gene="rbfA"
                     /locus_tag="PLD53_10890"
     CDS             complement(13..429)
                     /gene="rbfA"
                     /locus_tag="PLD53_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRC61466.1"
                     /translation="MTTANRGFSRKDRVSEQIRRELADVIVTELKDPRVGMVSITDVE
                     VTADYAHAKIYFSTLAGREQLEAVLTGLQNASGFLRRELGKRISIHMTPQLHFVFDQS
                     LERGADLTKLIQEAVAISELSDPTPITDSGDEQDQT"
     gene            complement(430..3159)
                     /gene="infB"
                     /locus_tag="PLD53_10895"
     CDS             complement(430..3159)
                     /gene="infB"
                     /locus_tag="PLD53_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRC61467.1"
                     /translation="MAQINVSQFATDLKMPAGALLEQLQKAGVAKNNADDLLSEQDKS
                     RLLEYLRRSHGEGGAKTKITLTRKETSEIRSQDSRGKSHMVQVEVRKKRVLVKRDAPE
                     NRAEIQPEIHAEAPAVEPQAPIAAPEPVVEIAVEPVVEPAVDLADDAAPQESAAPEIV
                     PDGDSKAAAGTKKVVTRASIIGEEQQRLRAEEDRRNSELRARQEAEYLEKQQRVDDLV
                     RLKLEAEEKAVAARAAEAAKKLAAQTASDKPAEDKTLHKPPAKPGSADKKTADKRGQW
                     KNEGANKRHGLRTRGAIGGTGWRDNKHGKRSTRGAETDEAHSFQAPTEAVVHEVHVPE
                     TISVSDLAHKMTVKATEVIKTLMKMGSMVTINQVLDQETAMIIVEEMGHKAFAAKLDD
                     PDAFIDDEAHKDAPQEPRAPVVTVMGHVDHGKTSLLDYIRRTRVASGEAGGITQHIGA
                     YHVETDRGMVTFLDTPGHEAFTAMRARGAKATDLVILVVAADDGVMPQTKEAIHHAKA
                     AGVPLIVAVNKIDKAEANPERVKQELVAEQVVPEDYGGDAPFISVSAKTGVGIDELLE
                     NVLLQAEVLELKAPVDAPAKGLIIEARLDKGRGPVATMLVQSGTLRQGDVLLAGEVFG
                     RIRAMLDENGKSIKEAGPSIPVEILGLSDVPAAGQEAVVLGDERKAREIALFRQGKFR
                     DVKLAKKQAANLETILDQMTEAEVKMLPLIVKADVQGSQEALVQSLQKLSTDEVKVNI
                     IHGAVGAISESDVHLAQASKAVIIGFNTRADAGARKAAESVGVDIRYYNIIYDAVDEV
                     KAALSGMLSPERREEISGLVEIRQVFRATKIGTIAGCYVLEGVVRRTSRARLLRDHIV
                     IWDGEFESLKRFKDDVKEVRSGFECGLSLKNFNDYQEGDQLEVYDVTEIARTL"
BASE COUNT          611 a          971 c          851 g          763 t
ORIGIN      
        1 acacgctgcc aactacgtct ggtcttgttc atcgccggaa tcagtgatcg gggttggatc
       61 ggacaactcg gaaattgcca cggcctcctg gatcaacttc gtgagatcgg ctccccgttc
      121 gagcgattga tcgaacacga aatgcaattg cggcgtcatg tgaatgctga tgcgtttacc
      181 caactcgcgg cgcagaaagc ctgaggcatt ctgcaggccg gtcaggacag cttccagttg
      241 ttcacgtccc gccagggtgc tgaaatagat cttggcgtgc gcataatccg ccgtcacctc
      301 aacgtcggtg atgctgacca tgccaacccg cggatctttg agttcagtga caattacgtc
      361 agccagttcc cggcgaatct gttcggaaac acggtccttg cgtgagaaac ccctgttagc
      421 cgtcgtcact tacagtgtcc gggcgatttc cgtcacgtcg tacacttcca gttgatcgcc
      481 ttcctggtag tcgttgaaat ttttcagcga caaaccacat tcaaagccag aacggacttc
      541 cttgacgtca tccttgaagc gcttgagcga ctcgaactcg ccatcccaga taacaatatg
      601 gtcgcgcagc agtcgcgcgc gcgaagtacg cctgacgacg ccttcgagca cataacagcc
      661 ggcgatcgtg ccgatcttgg tggcgcggaa aacctggcgt atctcgacca gaccggagat
      721 ttcttccctt ctctcgggag agagcatacc ggaaagcgca gccttgacct catctacggc
      781 atcgtaaatt atgttgtagt agcgaatatc gacacctaca ctctcggccg ccttgcgtgc
      841 accggcatcg gcacgcgtat tgaaaccgat gatgactgcc tttgacgcct gagcgagatg
      901 cacatcggac tcgctaattg cacccacagc gccgtgaatg atattcacct tgacttcgtc
      961 ggtcgaaagc ttttgcaagg actggacaag cgcttcctgc gagccctgca catcggcctt
     1021 gacgatgagt ggcagcattt tgacttcagc ttccgtcatc tgatcgagga tggtttcgag
     1081 attggcagcc tgcttcttgg ccagtttgac atcacggaac ttgccctgcc ggaaaagcgc
     1141 tatttcacgt gctttgcgct cgtcacccag gacaacggcc tcctgccccg cagcaggaac
     1201 atccgagagt ccgagaatct cgacaggaat cgacggcccg gcttccttta tcgatttgcc
     1261 gttttcatca agcatcgcgc ggatacggcc aaaaacctcg ccggccagca gaacgtcacc
     1321 ttggcgaagc gttccggact ggaccagcat ggtcgccacc ggaccacgcc ccttgtcaag
     1381 gcgcgcttca ataatcaggc ctttcgccgg ggcatcgaca ggtgccttca gctcgagcac
     1441 ttcggcctgc agcagcacat tttcgagcag ctcgtcgatt cccacgccgg tcttggcgga
     1501 gactgaaatg aacggagcgt caccgccgta atcttccgga acaacctgtt cggcaaccag
     1561 ctcctgcttg acacgctcag ggttggcttc ggccttgtca attttgttca ccgcgacaat
     1621 gagtggaact cccgccgctt tggcgtgatg aattgcttct tttgtttgcg gcatcacacc
     1681 atcgtccgca gcaacaacca ggataaccag gtcagtcgct ttcgcaccgc gtgcacgcat
     1741 cgctgtaaag gcttcgtgcc ccggtgtatc aaggaaggtc accatgccgc gatcggtttc
     1801 aacatgatag gcgccgatat gttgtgtaat gccgcctgct tcgccggaag ccacccgcgt
     1861 acgtcgtatg taatcaagca atgacgtctt gccatgatca acgtggccca tgacggttac
     1921 aaccggggct cgcggttcct gaggcgcatc cttgtgagcc tcgtcgtcaa tgaaggcgtc
     1981 cggatcgtcg agcttggctg caaaggcctt gtgacccatt tcctccacaa taatcattgc
     2041 ggtttcctga tccaggacct ggttgatcgt taccatcgaa cccatcttca tcagagtctt
     2101 gatcacttca gtggccttga ctgtcatctt gtgtgccaga tcggaaaccg agatcgtttc
     2161 cggcacgtgc acctcgtgga cgaccgcttc ggtaggcgcc tggaaggaat gcgcctcgtc
     2221 ggtctccgcg ccgcgagtgc tgcgttttcc atgcttgttg tcgcgccagc ccgtgccgcc
     2281 aatagcacca cgcgtcctca gcccatggcg cttgttggcc ccctcgttct tccactgccc
     2341 ccgcttgtca gctgttttct tgtcagccga gccgggtttg gcgggcggtt tgtgcagggt
     2401 cttgtcttcg gcaggtttgt cgctcgcagt ctgggctgca agcttttttg ctgcttcagc
     2461 ggcgcgggcg gcgaccgcct tctcttcggc ctcaagcttc agacgcacca gatcgtcaac
     2521 ccgctgctgc ttctccaggt actccgcctc ctgacgtgct cgcaattcgg aattgcgacg
     2581 atcctcttcg gcacgaagtc gctgctgttc ttcaccaatg atcgatgcgc gagtcactac
     2641 cttcttggtg ccggcggcgg ctttggagtc accgtccggg acaatttcag gcgcagcact
     2701 ctcctggggt gctgcatcat cagccaaatc gacggccggt tcaacgaccg gttcgactgc
     2761 aatttcaacg accggttctg gagcagcaat cggcgcctgc ggttcgacag cgggagcttc
     2821 ggcatgtatt tccggctgaa tctcagcacg gttctccgga gcatcgcgtt tgacgagaac
     2881 ccgcttcttg cggacttcaa cttgaaccat gtgcgattta ccgcgggaat cctgcgatcg
     2941 aatctcgctg gtctccttgc gtgtcagggt gatcttggtc ttggctccgc cctcaccgtg
     3001 tgagcgacgc agatactcca gcagccttga cttatcctgc tcggaaagca ggtcatcggc
     3061 attgttcttg gcgacgccgg ctttttgcaa ctgctcgagc agcgcacctg ccggcatctt
     3121 caggtcggtg gcaaattggg atacatttat ttgcgccatg tggaatcctc ttttctactt
     3181 actcaaacca gtgcgc
//
LOCUS       DAORPM010000268         3176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10369_length_3176_cov_14.008971, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000268 DAORPM010000000
VERSION     DAORPM010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10369_length_3176_cov_14.008971"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="PLD53_10900"
     CDS             complement(<1..120)
                     /locus_tag="PLD53_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRC61468.1"
                     /translation="MTSAERRGAAGLASIFAMRMLGLFLILPVFAVYAEHLPSG"
     gene            260..3112
                     /gene="uvrA"
                     /locus_tag="PLD53_10905"
     CDS             260..3112
                     /gene="uvrA"
                     /locus_tag="PLD53_10905"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRC61469.1"
                     /translation="MREIRIRGARTHNLKNINLDLPRNRLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQRMDKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLFARAGTPYCPEHQQALNSQTVSQMVDHVLALPVESRLMVMAPVIANRKG
                     EQLDLFAELRAQGFARLRVDGVIYEIDALPKLAKTRKHTVDVVVDRLRVRDDMRQRLA
                     ESFETALRHADGRAIAYEMDSGKEHLFSARFACPVCSYSLQELEPRIFSFNNPMGACP
                     SCDGLGVIQFFDPKRIVAQPALSLAAGAIRGWDRRNQFYFQLLTSLAAHYGFDVETPF
                     ARLDERIQKVLLYGSGREVIPFIYINERGNVTTREHSFEGIVCNFERRYKETDSVAVR
                     EELAKMISNKVCPGCDGARLREEARNVRIGTQPGALTLHEISGLPLAEAREHFLTLQL
                     VGNKAQVAEKILKEITSRLQFLINVGLDYLSLDRSAETLSGGEAQRIRLASQIGSGLT
                     GVMYVLDEPSIGLHQRDNNRLLETLKQLRDIGNTVIVVEHDEDAIRSADYVVDIGPGA
                     GVHGGFIVAEGDPDEVANNPGSMTGEYLSGKRRIEAPDKPRRFDAKRLLKLSGARGNN
                     LKNVTLELPVGLFTCITGVSGSGKSTLINDTLYAAAARHLYGSTAEPAEYDEIVGLDQ
                     FDKVISVDQSPIGRTPRSNPATYTGLLTPIRELFAGVPESRARGYGPGRFSFNVKGGR
                     CEACQGDGVIKVEMHFLPDIYVTCDVCHGKRYNRETLEVHYKGRTIHDILQMTVEQAR
                     EFFDAVPVVARKLQTLVDVGLSYITLGQAATTLSGGEAQRVKLALELSKRDTGRTLYI
                     LDEPTTGLHFQDIEMLLKVLHRLTDHGNTVVVIEHNLDVIRTADWIVDLGPEGGEGGG
                     RILAAGTPKVIGKTAGSYTGHYLKPLLVRKRSINIC"
BASE COUNT          689 a          918 c          913 g          656 t
ORIGIN      
        1 tccggatggc aggtgttcag cataaaccgc aaaaaccggc aagatcagaa acagacccag
       61 catgcgcatg gcaaatattg aggccaaccc ggctgcgcca cgccgctcgg ctgaagtcat
      121 cgggtcatgg gttggagata acatcaggtc ggcaatggaa agctggcgaa aggaggaaca
      181 aggattggtt caagcggtcg gagttgcgta tattaacagg tttgctttac ctccctggca
      241 gcactcccca atggatgaga tacgagaaat caggatccgc ggtgcacgca cgcacaactt
      301 gaagaatatc aatctcgatc tgccacgcaa ccggctgatc gtgattaccg gcctctctgg
      361 ttccggaaaa tcctcactgg cattcgatac gctttacgcc gaaggccagc gccgctatgt
      421 cgaatcattg tctgcgtatg ccagacaatt cctgcagcgc atggacaagc ccgacgtcga
      481 cctgatcgaa gggttgtcgc cggccatctc gatcgagcag aaggcgacca gccataaccc
      541 gcgttcgacg gtcggcacgg tgaccgagat tcacgactac ttgcgcctgc ttttcgctcg
      601 cgccggcact ccctactgcc ccgagcatca gcaagcgctg aattcgcaga ccgtttcgca
      661 gatggtcgat catgtgctgg cactacccgt cgaatcacgt ttgatggtta tggcgccggt
      721 tatcgccaac cgcaagggtg aacagctcga cttgtttgcc gaattgcgtg ctcagggttt
      781 cgcccggttg cgggtcgatg gtgtgatcta cgaaatcgat gcgttaccga agctggccaa
      841 gacgcgcaag cataccgtgg atgttgtggt agaccgcctc cgggtgcgtg atgacatgcg
      901 gcagcgtctg gcggaatcct ttgaaaccgc gctgcgccac gccgacggtc gcgcaatcgc
      961 ttacgagatg gattccggaa aggagcatct gttttcggcc agatttgcgt gcccggtatg
     1021 ttcctattcg ctgcaggaac ttgagccgcg catcttctcg ttcaataacc cgatgggcgc
     1081 ctgcccgagc tgcgacgggc tgggcgtgat ccagtttttc gatcccaagc gtattgtcgc
     1141 ccagcctgcc ctttcacttg ctgccggcgc gattcgcggc tgggatcggc gcaaccagtt
     1201 ctacttccag ttgcttactt cgctggccgc acattacggc tttgatgtcg agacgccgtt
     1261 cgcccggctg gacgagcgca tccagaaggt cctgctctac ggatcgggcc gcgaagtcat
     1321 tccattcatc tacatcaacg agcggggaaa tgtcacgacg cgcgagcata gcttcgaggg
     1381 catcgtctgc aattttgaac gccgctacaa ggaaacagac tccgtagctg tgcgcgagga
     1441 actcgccaag atgatcagca acaaggtctg tccgggatgc gacggtgcac gacttcgcga
     1501 agaagcgcgc aatgtgcgca tcggaacaca gccgggcgca ctcaccctgc acgaaatcag
     1561 tggcttgccc ctggccgagg cgcgagaaca ttttctgacc cttcaactgg ttggcaacaa
     1621 ggcgcaggtc gctgaaaaga tcctcaagga aatcaccagc cgcctgcagt ttctgatcaa
     1681 cgtcggcctt gactatctgt cgctcgatcg ctccgcagaa acgctctccg gcggtgaggc
     1741 gcagcgcata cgactggcct cgcagatcgg ttcgggattg accggcgtca tgtacgtcct
     1801 tgatgagcct tcgattggtc tgcaccagcg cgacaacaac cggctgctgg agacactcaa
     1861 gcaactgcgt gatatcggca acacagtgat cgtcgtcgag catgacgaag atgcgatccg
     1921 tagcgcggac tacgttgtcg atatcggtcc cggggccggt gttcacggcg gtttcatcgt
     1981 cgccgaaggt gatccggacg aggtggccaa caatccgggc tcaatgacgg gggaatatct
     2041 gtctggcaaa cgccgtatcg aggcgccgga caagccgcgg cgattcgatg ccaagcggct
     2101 gctgaaattg agcggtgcgc gcggcaataa cctcaagaac gtgacgctgg aactaccggt
     2161 tggattgttt acctgcatca ccggtgtttc cggctccggc aaatcaacat tgatcaacga
     2221 cacgctctac gctgcggctg cgcgacatct ctacggctcg acggcagaac ctgcggagta
     2281 cgacgaaatc gtcggccttg atcagtttga caaagtcatc agcgtcgacc agtcgccaat
     2341 tggccgtacg ccgcgctcca acccggccac ctatactggt ctgttgaccc cgatccgcga
     2401 gttgttcgcc ggtgtgccgg agtcgcgtgc gcgcggttac gggcctggtc gcttttcctt
     2461 caacgtcaag ggcgggcgct gcgaagcctg ccagggcgat ggcgtaatca aggtcgagat
     2521 gcacttcctt cccgacatct acgtcacctg cgacgtctgc cacggtaagc gctacaaccg
     2581 cgaaacgctt gaagtgcatt acaagggcag gactatccac gacatcctgc aaatgaccgt
     2641 cgagcaggcg cgtgagttct tcgatgccgt tccggtcgtc gcacgcaagc tgcaaacctt
     2701 ggtcgacgta ggcttgtcgt acatcacgct cggccaggcg gcaacaacgc tttcaggcgg
     2761 tgaggcccag cgcgtaaaac tggccctcga attgtccaaa cgcgatactg gacgaacact
     2821 ctatattctt gacgagccga ccaccggcct acatttccag gatattgaaa tgctgctcaa
     2881 ggtgcttcat cgtctgacag atcatggcaa cacggtggtt gttattgaac acaacctgga
     2941 tgtgatacgg acagcggact ggatcgtcga tctcgggccc gagggcggcg agggcggcgg
     3001 tagaatacta gccgcgggta cgcccaaggt cattggaaaa actgcgggga gctatacggg
     3061 acactacctg aagccactgc tggtgcggaa aagatcgata aacatttgtt gacgttgtca
     3121 ctgagccaca aaacgccact tttttttgat cttctttacc ggttccagac tcaaac
//
LOCUS       DAORPM010000269         3120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10667_length_3120_cov_9.677325, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000269 DAORPM010000000
VERSION     DAORPM010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10667_length_3120_cov_9.677325"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="PLD53_10910"
     CDS             <1..138
                     /locus_tag="PLD53_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HRC61470.1"
                     /translation="RYAVRNGGYLRILKCGFRIGDNAPMAFVELLDQPEATAVAEADA
                     A"
     gene            complement(204..2057)
                     /locus_tag="PLD53_10915"
     CDS             complement(204..2057)
                     /locus_tag="PLD53_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRC61471.1"
                     /translation="MRRSSRSSSDYPPAAGQTAGDSHLWRTLNSLAPYLWQYKWRVLL
                     ALSCLFSAKLANVAVPLVFKEMIDGLTPPQQVLALPALLLALYGALRFSTSLFTELRE
                     ILFARVTQQAVRRIALEVFRHLHALSLRFHLERQTGGVSRDIERGSRSISSLISYTLY
                     SILPTLVEMSLVLGILFFKYDGIFVVITLVSLTAYVVFTIKVSNWRIGLRRRVNDTDS
                     AANTRAIDSLLNYETVKYFNNEEYEARRYDAHMRQWEDAATKSQISLSWLNLGQQIVI
                     ALGVTAMMWRAAAGVVDGKMTIGDLVLVNAFLIQLYMPLSFLGVIYREIRQSLTDIER
                     MFDLLAVHREIADAPDATDLHAESVSVSFESVEFSYDPKRKILFGIGFDIPSGHTVAV
                     VGESGSGKSTLARLLYRFYDVDAGRICINERDLRQLTQASLRAMIAIVPQDTVLFNDS
                     IYYNIQYGRPEASRADVLAAARSAQLTEFIDLLPDGYETRVGERGLKLSGGEKQRVAI
                     ARALLKNPPLLIFDEATSALDSKTEKAIQASLDNAARGRTTLIIAHRLSTVMNADEIL
                     VMSGGRIVERGSHWSLLVANGHYARMWELQQQEDEETEEVNSGTANVRSSL"
     gene            2218..2625
                     /locus_tag="PLD53_10920"
     CDS             2218..2625
                     /locus_tag="PLD53_10920"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRC61472.1"
                     /translation="MSDQTNKPVSMPYGSPALRVMPMPADANQYGDIFGGWIMSQVDI
                     AGATAAMRCARGRVATVSVNSFVFKQPVSVGDIVSFFAEVVATGRTSIKVNVEVFAER
                     NPVQPMTVKVTEATLTYVAIDRQGSKRALTADE"
     gene            2720..>3120
                     /locus_tag="PLD53_10925"
     CDS             2720..>3120
                     /locus_tag="PLD53_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRC61473.1"
                     /translation="MSALIVRKAAVLGAGVMGAQIAAHLANAEIPVVLFDLPAKVGDP
                     NGIVMRARDTLSRLEPSPLATAERIDYIDIANYAQHLDRLRECDLVIEAIAEKAELKA
                     DLYRRIAPFILPNAIVASNTSGISIKLLSGS"
BASE COUNT          676 a          856 c          938 g          650 t
ORIGIN      
        1 cgctatgccg tacgcaatgg tggttatctg cgcatcctga agtgcggatt tcgaatcgga
       61 gacaatgcgc cgatggcttt tgtcgagctg ctcgatcaac cggaggctac ggctgttgcg
      121 gaggctgacg cagcgtaagc gcatctggta gttgttgcgt tgtcgaaaga gccgggcttg
      181 cccggctttt tttccgctca ggtttagagg gacgagcgca catttgctgt tccgctgttt
      241 acctcctccg tttcttcatc ttcttgttgc tgaagctccc acatccgcgc gtagtgaccg
      301 ttcgctacga gcaatgacca gtgcgatccg cgctctacaa tacgccctcc agacatgacc
      361 agaatctcgt cggcattcat gacggtcgaa agccggtgcg caattatcag cgtggtacgg
      421 ccgcgcgctg cattgtccag actggcctga attgcctttt ctgtttttga gtcgagcgcc
      481 gaagtagctt cgtcgaaaat cagcagcggc ggattcttga gcagggcacg cgcgatggct
      541 acgcgctgtt tttcgccgcc ggaaagttta aggccacgtt ccccgacccg ggtttcatac
      601 ccatcgggca ggaggtcgat gaattccgtg agttgagcgg accgcgccgc cgccagcaca
      661 tctgcgcgac ttgcttccgg ccgcccatac tggatgttgt agtagatgct gtcgttgaac
      721 agcaccgtat cctgcggaac gatagcgatc attgcgcgca gactggcttg tgtcagttgc
      781 cgcaaatcgc gctcattgat gcagatgcgg ccagcatcga cgtcataaaa gcggtagagc
      841 aatcgagcca gcgtcgattt gcccgacccg gattcgccga cgacggcgac ggtgtgcccc
      901 gacggtatgt caaagccgat cccgaacagg atcttgcgct taggatcgta cgagaactca
      961 acggactcga agctcacgga aacggattcc gcatgcaggt cagtggcatc cggagcgtcg
     1021 gcaatctcac gatgcaccgc cagcaaatcg aacatgcgct cgatatctgt cagcgattga
     1081 cgaatttccc tgtagatcac cccgagaaaa ctcaacggca tgtagagctg gatcaggaag
     1141 gcgttcacca gtacaagatc gccaatggtc atttttccgt cgaccacacc ggctgcggcg
     1201 cgccacatca tcgcagtcac accaagagcg atgacaatct gctggccgag gttgagccag
     1261 gacaaggaaa tctggctctt ggtggccgca tcttcccact ggcgcatgtg cgcgtcgtaa
     1321 cgtcgggctt catactcttc gttgttgaag tatttgaccg tctcgtaatt gagcagactg
     1381 tcaatggcac gggtgttggc cgccgagtcg gtatcattga cgcgacgccg caggccgatt
     1441 cgccagttgc tcaccttgat cgtgaacacg acatacgcgg taagcgacac cagggtgatc
     1501 accacaaaaa tcccatcgta tttgaagaac agaatgccga gcacgaggct catctcaacc
     1561 agtgtcggca ggatcgaata gagcgtgtaa ctgatcaggc tcgatatcga acggctgccc
     1621 cgttcgatat cacgtgacac gccacccgtc tgccgttcaa gatggaaacg cagcgatagc
     1681 gcgtgaaggt ggcgaaacac ttccagcgcg atacgccgca ctgcctgctg ggtcacgcgc
     1741 gcgaagagga tttcacgcag ctcggtgaac agggaggtag aaaaacgcag cgcgccgtag
     1801 agggcgagta gcagggcggg cagagccagc acctgctgag gaggcgtcag cccgtcgatc
     1861 atttccttga agacaagcgg gacggcaaca ttggcaagtt tggcgctgaa caggcaggac
     1921 aaggcgagca ggactcgcca tttgtactgc cacaggtagg gggcaaggct gttcagggtc
     1981 cgccaaaggt gggaatcgcc cgccgtctgg ccagcggctg gcgggtaatc ggaggaactg
     2041 cgagaagaac ggcgcatatt gattcatcaa tttcggtacg atggagtagt gtcgagtatg
     2101 cggcctagtc tggtcggcgc tcaacagata attgtcgaaa tgatggcttg ttgggacgct
     2161 gaattgttgc attcgaacca gcgccagctt ttctgcccaa gagaacaagg aatcgtaatg
     2221 tccgaccaaa ccaacaaacc ggtttcgatg ccatatggct cgccggcgct gcgcgtgatg
     2281 ccgatgccgg ccgacgccaa tcaatacggc gacatcttcg gcggctggat catgtcgcag
     2341 gtcgacatcg ccggtgcgac ggcggccatg cggtgcgccc gcgggcgcgt ggcgacggta
     2401 tcggtcaatt cgtttgtatt caagcagccg gtttcggttg gcgacattgt cagtttcttt
     2461 gccgaagtgg ttgcaactgg tcgaacctcg atcaaggtaa acgtcgaggt attcgccgag
     2521 cgaaatcccg tgcagccgat gacagtcaag gtgaccgagg cgacgcttac ctatgttgca
     2581 atcgacaggc agggcagcaa gcgcgcgctg acggctgacg aatgatcaac ggaagcttca
     2641 ctcaagtgct gttgcagggg aacagcagcg gtactcgcca gtaccgttgc cagggacgaa
     2701 ctcggaggga gggggaatct tgagcgctct tatagttcgc aaggcggcgg tgctcggggc
     2761 cggtgtgatg ggtgcgcaga ttgccgcgca tctggctaat gccgaaatac cggtggttct
     2821 gtttgacctg ccggccaaag tgggtgatcc gaacgggatc gtcatgaggg ctcgggatac
     2881 gctcagcagg cttgagccgt cgccactcgc gactgcagag cgtatcgact acatcgatat
     2941 cgccaactac gcgcaacatt tggatcgact cagggagtgc gacctggtca tcgaagccat
     3001 tgccgagaag gctgagttga aagccgatct gtaccgcagg atcgcaccct tcatcttgcc
     3061 gaatgcgatc gttgcctcga acacctccgg gatctcgatc aaacttcttt ccgggagctt
//
LOCUS       DAORPM010000270         3098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10788_length_3098_cov_10.878081, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000270 DAORPM010000000
VERSION     DAORPM010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10788_length_3098_cov_10.878081"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLD53_10930"
     CDS             complement(<1..775)
                     /locus_tag="PLD53_10930"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRC61474.1"
                     /translation="MNSPTSRTRLSPGHRLRESMTHSDLIPFIGVYDVFSATLAGRHF
                     DSLFVSGFGFAASHYGLPDIGFITWTDIVDYVQRLRTVLPFHNLLVDIDDGYCDPEVA
                     CHVVSVLEAAGASGVVLEDQKRPRRCGHFEGKQIMELDEYLAKLHAVLATRRDMVVIA
                     RTDASDLDDIARRVEAFAAAGADAVLVDGLTSLDVVRSLSKRVDRPFCFNQIAGGKSP
                     VCTQSELKEAGVSLVIYSTPCLFAAQAAIDEAMADLKARD"
     gene            complement(772..1032)
                     /locus_tag="PLD53_10935"
     CDS             complement(772..1032)
                     /locus_tag="PLD53_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRC61475.1"
                     /translation="MSADRPQRLKNLIEFLRTIQKPTKRIENVGVDDSLFATGLVDSL
                     AIIQIVLYLETTYGIDFGASGIDPERVSTMTSILDLIEETRQ"
     gene            complement(1022..2548)
                     /locus_tag="PLD53_10940"
     CDS             complement(1022..2548)
                     /locus_tag="PLD53_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRC61476.1"
                     /translation="MYFDLNVGNLAEPISGRSWTRQEIPGQVARRVARFQRHGLSRGD
                     RVFLPFGNSLEFFAELLAIWKLGACAVPIDARLTAFEIKTLVAAAQPRLAVVDHLTET
                     MIRQALADASVPAVETTETGDEEATAGLSQLDDDALILFTSGSTGAPKGVVHTHRSLR
                     ARWMGIHDQLGLVPFARSLCLLPTHFGHGLICNCLYPWLAGQDLFITPPFRPEIIMRL
                     GRLIDDNRITFMSSVPAIWKLATKLGKPPQAGTLQRVHCGSAPLSAHAWEEIRAWTGT
                     RNVCNAYGITETGSWVAGLSDADCAAEDGLIGVGWGAVIKVLRTSDTERPLRPDDECA
                     AGESGFVWLNTPALMKGYFQRDDLTALAVSEGWFLTGDIGFIDEHGRLVLRGRERDEI
                     NKGGMKIYPADIDTVVERFAGASDVCTFALDDEIYGQAVGLAVVLGNSEDATIRALYE
                     WMKSQLAEHKMPVRWWVIDEIPRTSRGKINRDAVKAFCVTRDALDLARILDGESRDER
                     "
     gene            complement(2572..>3098)
                     /locus_tag="PLD53_10945"
     CDS             complement(2572..>3098)
                     /locus_tag="PLD53_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61477.1"
                     /translation="IAILRIFGFNVFRNTYKPLLAESIVEFWNRYYYYFKEIMANFFF
                     LPTFTQLGRQLRNWPTLRLFAAVFAAAFIGNTYYHLIKLGDMMVQGQVFEGLYALRSR
                     IFYCLLLALGIFVSMLREQRRGGRPPAQGQANRLLRIAGVWTFFSLIYIWNVGSGAPF
                     IPRLNFFLSLFGIA"
BASE COUNT          677 a          928 c          921 g          572 t
ORIGIN      
        1 cgtcacgcgc cttgaggtcc gccatggcct cgtcgatcgc cgcctgggcg gcgaacaggc
       61 acggtgtgct gtaaatcacc agagaaacgc cggcctcttt caactcgctc tgcgtacata
      121 ccggggactt gccgccggca atctgattga aacagaaggg acggtcaacc cgctttgaca
      181 aggagcgaac cacatccaga ctggtcagcc catcgaccag caccgcatcc gccccggctg
      241 cggcaaacgc ctcaacgcga cgcgcaatat cgtccagatc tgaggcatcg gtgcgcgcaa
      301 tcaccaccat atcgcgccgg gtcgccagaa cggcgtgcag cttggccaag tattcatcga
      361 gttccatgat ctgcttgccc tcgaagtgac cgcagcggcg cggccgcttc tgatcctcga
      421 gcaccacccc ggacgcccca gcggcctcga gcaccgacac cacatggcaa gccacttcgg
      481 ggtcacagta gccgtcatcg atatcgacca gcagattatg gaagggcagg acggtgcgca
      541 aacgctgcac atagtccacg atatccgtcc atgtgatgaa cccgatatcg ggcaagccgt
      601 aatggctggc ggcaaaacca aaaccgctga caaacaggct gtcgaaatga cgccccgcga
      661 gcgtcgccga aaagacgtca taaaccccga tgaacgggat cagatcactg tgggtcatcg
      721 actcccggag acgatggcct ggcgacaagc gcgtgcgcga cgtgggtgaa ttcattggcg
      781 ggtttcctca atcaggtcaa gaatgctggt catcgtactc acccgctccg ggtcgattcc
      841 gctggcgccg aaatcaatcc cgtaggtggt ttcaaggtac aggacaatct ggatgatggc
      901 caacgagtcc accagccccg tcgcgaacag gctgtcatcg acgccaacgt tctcgatccg
      961 tttggtgggc ttctgtatgg tgcgcagaaa ctcgatcaga ttcttcaggc gttgcgggcg
     1021 atcagcgctc atcgcggctt tctccgtcaa gaatccgggc cagatcgagc gcatcccgag
     1081 tgacgcaaaa ggccttgacc gcatcccggt tgatcttgcc gcgcgacgtt ctgggaattt
     1141 cgtcgatcac ccaccagcgc accggcatct tgtgttccgc cagttgcgac ttcatccact
     1201 cgtacagtgc ccggatcgtc gcgtcctcgg aattgccgag cactaccgcc aggcccaccg
     1261 cctggccgta aatttcatcg tcgagtgcaa aagtgcagac gtcactggcg ccggcgaagc
     1321 gttcgacgac ggtatcgata tcggccgggt agatcttcat gccgcccttg ttgatttcgt
     1381 cgcgctcgcg gccgcgcagc accaatctgc cgtgctcgtc gatgaagccg atgtcgccag
     1441 tcaggaacca gccctcgctg acagcgaggg cggtcaggtc gtcgcgctgg aagtagcctt
     1501 tcatcagggc tggtgtgttg agccagacga aaccggattc gcccgcagcg cattcgtcat
     1561 cgggacgcaa tggtcgctcc gtgtcggagg tgcgcaagac cttgatcacg gcgccccagc
     1621 cgacgccgat caggccgtct tctgcggcgc agtcggcgtc gctcagacca gcgacccaac
     1681 tgccggtctc ggtgatgccg tacgcgttac agacgttgcg ggtgccggtc caggcgcgga
     1741 tttcttccca ggcgtgcgcc gagagcggcg ccgaaccgca atgcacccgt tgcagggtgc
     1801 cggcttgcgg cggtttgccg agtttggtcg cgagtttcca gatggcgggt accgaagaca
     1861 tgaaggtgat gcggttatcg tcgatcagcc ggcccaggcg catgatgatt tccgggcgga
     1921 agggcggggt gatgaacaga tcctgtccgg ccagccaggg gtacaggcag ttgcagatca
     1981 gtccgtggcc gaagtgcgtt gggagtaggc acagcgagcg ggcaaaggga acaaggccca
     2041 actggtcatg gatccccatc cagcgggcgc gcagcgagcg gtgggtatgc accacacctt
     2101 tgggcgctcc ggtcgatccg gaggtgaaca ggatcagggc atcgtcgtcg agttggctta
     2161 gcccggccgt cgcttcttcg tcaccggttt ccgtagtttc gacagccgga acgctggcgt
     2221 cggccagcgc ctgcctgatc atcgtttcgg tcagatggtc aacgaccgcc agccggggct
     2281 gtgccgcggc gaccagggtc ttgatttcaa aggcagtcag gcgggcgtcg atgggtacgg
     2341 cgcaggcacc gagtttccag atggccagca gttcggcaaa gaattccagg ctgttgccga
     2401 agggcaggaa gacgcggtct ccccgcgaca gtccatggcg ctggaagcgg gcgacccgcc
     2461 gtgcgacctg gcccggtatc tcctgacgcg tccatgaccg tccgctgatc ggttcggcaa
     2521 gatttccaac attcagatca aaatacattc gaactcactc cctaaagatg ttcatgcaat
     2581 tccgaaaaga ctcaaaaaga aattcagacg gggaataaag ggggcaccac tgccgacatt
     2641 ccagatgtaa atcaggctga aaaaagtcca aacgccggct atcctcaaca agcggtttgc
     2701 ttgaccctgt gccggcgggc gtcctccccg gcgttgctcg cgcagcatgg acacaaaaat
     2761 acccaaggcc agcaacaaac aatagaagat tcgtgaacga agcgcataca ggccctcgaa
     2821 aacctgaccc tgcaccatca tatcgccaag cttgataagg tggtaatagg tgttgccgat
     2881 aaaggctgcc gcaaaaaccg ctgcaaacag acgtaaagtg ggccaattgc gcaattgcct
     2941 tcccaactgg gtgaacgtgg gaaggaaaaa gaaattggcc atgatctcct tgaaatagta
     3001 gtaatagcgg ttccagaact ccacaatcga ttctgccagg aggggcttgt aggtattgcg
     3061 gaatacgttg aaaccaaaaa tccgcaggat ggcaatga
//
LOCUS       DAORPM010000271         3095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10800_length_3095_cov_16.246711, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000271 DAORPM010000000
VERSION     DAORPM010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10800_length_3095_cov_16.246711"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..>3095
                     /locus_tag="PLD53_10950"
     CDS             169..>3095
                     /locus_tag="PLD53_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HRC61478.1"
                     /translation="MLGLAVPHSWNDPETQDLWSLSTDESALLPGMTDKGRLGFAIQL
                     KFMQLHGRFPERHDEIDPNATQWLAAQLGTTTEALSLYALNGRQGQRHQRTIRVFLGY
                     RPATGDDLKQLNHWLCSEALPFDPQARHGRDLALDWCRIHHLEPPASDELNRTIRSAV
                     RSYEAQQQAVIYSRLSASSKAAINRLLGEDDTDPDEADNENAKSISFSSLKTDPGKAS
                     LDSLLVAIAKLKCIDEIGLAPEVFTDVPAKFIDQFRQRCSTESIRELRRHPVAIRYSM
                     VAMFCWRRQQQLTDSLIDLLLQVIHNLGTRAEKKIDKKQFVAFKKIRGKARLLFKLAE
                     ATVDQPEGIIKEVVYPVVSKKTLEELVAEFKTMGFDFEREVQESMRSSYGHHYRSMLM
                     PVLDALIFQSNNTVHRPVIEALAVLKANRDSRQQYYNAADVPLEGIVSTKLRDVVVEQ
                     DKNGKDRVNRINYEICVLRALRKRLGTKEIWVDGADRYRNPEQDLPADFADMRDSYYA
                     MLDVPKDVAVFIEQIQSTLRQGLKTLNDGLPDNPKVRLRQQGKNLIHLSPLEAQTEPP
                     NTAALKREIGQRWSDVELIDIVKEVDLRVNFTAAFRTTASREALDPALLQRRLLLCLF
                     GIGTNVGLKRVASQQPSVNFEELRYVKRRFVQKEALRAAIGEVVNGIFQIRNPAIWGS
                     ATTACAADSKKFGAYDQNLMTEWHARYGGRGVMIYWHVDTNSTCIYSQLRRCSSSEVA
                     AMMEGVLRHCTDMEIERQYVDSHGQSEVAFAFSYILGFDLLPRLKAIARQKLSLAAST
                     DAAQYPNLEPILTRAINWELIRQQYDEIIKYTTALKLGTAEPEAILRRFTRAGAAHPT
                     YKALTELGKAIKTIFLCRYLHEESLRREIHEGLNVVENWNSANNFIFYGERGEFATNR
                     VADQELAVLSLHLLQVCLVYVNTLFIQEVLTEPAWRDRMTVDDWRGLTPLIYHHVNPY
                     GRI"
BASE COUNT          721 a          898 c          855 g          621 t
ORIGIN      
        1 ggtaggacga ataaaagcag gtcgaaaaat acacaaggga acggtttagt aatttggccc
       61 agtgggagcg aagctgagat ttcgcgacat ataagatact actaatagac tctgtcaatt
      121 atctccccgg cgggctcctg catttctata attcggcttc cttgatcctt gctaggcctt
      181 gccgtgcccc attcatggaa cgatccagaa actcaggact tgtggagctt gtccaccgac
      241 gagtccgcct tgttgccagg catgactgac aaaggtcggc tcggtttcgc aatccagctc
      301 aagttcatgc agttgcacgg gcggtttccc gagcgacatg acgaaatcga tcctaacgca
      361 acgcaatggc tggctgcgca actcggcaca acgacagaag cgctgtcttt gtatgcgcta
      421 aatggccgtc aaggacagcg gcaccagcgg actattcgag tatttcttgg ctaccgtccg
      481 gctactgggg atgacttgaa acagctcaat cattggctat gcagcgaggc actcccgttc
      541 gatccgcaag cccgccatgg ccgagacctt gcgctggact ggtgtcgtat tcatcacttg
      601 gagccgcccg cgagcgatga actcaaccgc accatccgtt cggcagtacg tagttatgag
      661 gctcagcaac aagcggtcat ttactcgcgc ttaagcgcaa gcagcaaagc cgcgatcaac
      721 cgattgctgg gcgaagacga taccgatccc gacgaagctg acaacgaaaa cgccaagagc
      781 atctccttca gcagcctcaa gaccgatcca ggcaaagcca gtctggatag cctgctggtc
      841 gccattgcca aactcaagtg tatcgacgag attggtttgg cgccggaggt ttttactgat
      901 gttcccgcca aattcataga ccagttccgg caacgctgct cgactgaatc catccgagaa
      961 ctacggcgtc accccgttgc aatccgctac agcatggtgg ccatgttttg ctggcgacgc
     1021 cagcaacagc tcacggattc gctgatcgac ttgctcttgc aagtgattca caacctgggc
     1081 acccgtgccg agaagaagat cgacaagaaa cagttcgttg ccttcaagaa aattcgcggc
     1141 aaggcccgtc tcctgttcaa gctggccgag gccaccgtcg atcagccgga gggaatcatc
     1201 aaagaggtcg tctatccggt ggtgtccaag aaaaccttgg aagaactggt cgccgaattc
     1261 aagactatgg gctttgactt cgaacgggaa gtacaggagt ccatgcggtc ctcttatggc
     1321 catcactacc ggagcatgct gatgccggtg ctggatgcgc tgatctttca gtccaacaac
     1381 accgtccatc gcccggtcat tgaagccctg gctgtcctca aggccaaccg ggatagccga
     1441 cagcagtatt acaacgccgc cgacgtgccg ctcgaaggca ttgtgtcgac gaaattgcgc
     1501 gatgtcgtgg tggaacagga caaaaacggc aaagaccggg tcaatcgcat caactatgaa
     1561 atctgtgtcc tgcgtgcctt gcggaaacgc ctgggcacca aggaaatctg ggtcgatggt
     1621 gcggatcgct atcgcaatcc agaacaggat ctgccggccg attttgccga tatgcgcgac
     1681 agctattatg cgatgctgga cgtgcccaag gacgtagcgg tcttcattga gcagatccag
     1741 tccaccctgc ggcaaggcct gaaaacactg aacgacggcc ttccggacaa cccgaaggtg
     1801 cgcctgcggc aacaaggcaa gaacttgatc cacctctcgc cgctggaggc gcagacggag
     1861 cctcccaata ccgccgcgct caagcgcgag atcggccaac gctggtcaga cgtcgagctg
     1921 atcgacatcg tcaaggaagt cgacttgcgg gtcaacttca ccgccgcgtt ccgtaccacc
     1981 gccagccgcg aagcgctcga cccagcccta ttgcagcgcc gcctgctgct ctgcctgttt
     2041 ggcatcggca ccaatgtggg cttaaaacgg gtggccagcc aacaaccgag cgtcaacttc
     2101 gaggaattac gctacgtcaa acgccgcttc gtgcagaaag aggcgttacg tgccgcaatc
     2161 ggcgaagtcg tcaatgggat cttccagatc cgtaacccgg caatctgggg cagcgcaacc
     2221 accgcctgcg cggctgattc caaaaagttc ggcgcttacg atcagaatct gatgaccgaa
     2281 tggcacgcgc gctatggtgg gcgcggtgtc atgatctact ggcacgtcga caccaattcg
     2341 acctgcattt actcacaatt gcgccgttgc tcgtcgtcgg aagtagccgc catgatggaa
     2401 ggcgtgctgc gccattgcac ggacatggag attgagcgcc agtacgtcga tagccacggg
     2461 caaagcgaag tcgcctttgc cttcagctac atcctgggct tcgacctgct gcctcgactc
     2521 aaagcgatcg cccgacaaaa gctcagtctg gcggcaagta cggatgccgc acaatacccc
     2581 aacctcgaac cgatcctgac gcgggccatc aattgggagc tgattcggca gcagtacgac
     2641 gaaatcatca agtacaccac cgcgctgaag ctgggtacgg ccgaaccgga agccatcctg
     2701 cggcgcttta cccgtgcggg tgcggcgcat ccgacctata aggcactgac ggaattgggc
     2761 aaggcgatca agaccatttt cttgtgccgt tacttgcacg aagagtcgct gcggcgcgaa
     2821 atccacgagg ggctcaatgt ggtcgaaaac tggaacagcg ccaacaactt tatcttctac
     2881 ggcgagcgcg gcgagttcgc caccaatcgt gtggccgatc aggagctggc ggttttgtcg
     2941 ctccatttgc tgcaagtctg tctggtgtac gtcaatacgc tgttcattca ggaagtgctg
     3001 acggaaccgg cgtggcgtga tcgcatgacg gttgatgatt ggcgcggact cacgccgctg
     3061 atctatcacc acgtgaatcc gtacggccgc attga
//
LOCUS       DAORPM010000272         3092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10818_length_3092_cov_10.855449, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000272 DAORPM010000000
VERSION     DAORPM010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10818_length_3092_cov_10.855449"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLD53_10955"
     CDS             <1..582
                     /locus_tag="PLD53_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRC61479.1"
                     /translation="FGSEQGVGTQLVGTNTETLKREPKAGKEPYSDQALALAQNQNQP
                     KATESASAAAANSEAKPEPPPAAIALSGDEIAWAWPATGKIIGTFSEGSNKGIDIAGK
                     AGDPVLAAGDGKVIIANFLRGYGKLVIVKHNKTYVSVYGHNRTLLVKEGQLVVKGQKI
                     AEMGNTDADQVKLHFEIRSKGKPLDPLKFLPQR"
     gene            585..1544
                     /gene="rpoS"
                     /locus_tag="PLD53_10960"
     CDS             585..1544
                     /gene="rpoS"
                     /locus_tag="PLD53_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HRC61480.1"
                     /translation="MPGKPDSDSGFAEDEIPQERGPAEEGFLAESEDEANTVTPEVEL
                     LNDVTQHYLNEIGARPLFTPEEEFDWACRAHGGEFVARQKMIEHNLRLVVNIAKHYLN
                     RGIPLLDLIEEGNLGLIHAIEKFDPWRGFRFSTYATWWIRQSIERAIMNQSRTIRLPV
                     HVVKEINLVLRALRHLELANGREIHLEQVAHLIDRPIEEVRRALALNEHISSLDAPLE
                     IDPNHTVADAISDDNVLSPESMLQSSEVCNLVREWVEHLPEKQRRVLERRYGLWGGEI
                     STLEEVAADLNLTRERVRQIQIEALDQLRRVIKRGGMTRENLL"
     assembly_gap    1737..1836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1837..2811
                     /locus_tag="PLD53_10965"
     CDS             <1837..2811
                     /locus_tag="PLD53_10965"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRC61481.1"
                     /translation="GIHAHNDCGMAVANSVTAVENGARHVQGTYIGFGERCGNANLST
                     IIANLGVKRDFECLPDNQYRNITRVARHIAAISNLALNERDPYVGNCAFAHKGGMHID
                     GVNKASHSFEHINPDLVGGQRRFLMSEVSGRRMILEKIWEVDASITKDDAVTDSIIKR
                     LKELEHEGYQFEGAESTFELVIRKQLGKYRPFFELEHFKIIGEQPTNGEYSSSAIIKV
                     RVDGTDEITAAEGDGPVHALDCALRKALDKFFPELSNVHLTDYKVRVINGRDATAAKV
                     RVLLQSTDGDAVWTTVGVSTDIIEASWIALVDSIEYKLLVLEEAKCPM"
     gene            complement(2864..>3092)
                     /locus_tag="PLD53_10970"
     CDS             complement(2864..>3092)
                     /locus_tag="PLD53_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61482.1"
                     /translation="MRLCACREAYPRAECAKQAAVTAVAMARVKIKAIGETGRIEHSE
                     IYGFRYRVPGQIKPAGISRARLTGPAFAAFV"
BASE COUNT          714 a          757 c          864 g          657 t
ORIGIN      
        1 ttcgggagcg aacaaggcgt cgggacgcag ctggtgggca ccaataccga gaccttgaaa
       61 cgtgagccga aggctgggaa ggagccctac tcggatcagg ctctggctct ggcgcaaaat
      121 cagaaccagc caaaggctac cgagtcggcg tccgctgctg cggccaattc tgaagcgaaa
      181 ccggagcccc cgcccgccgc aattgcattg agcggtgatg aaattgcatg ggcctggccg
      241 gccactggca agataatcgg cacattcagc gagggcagca acaaggggat agatatcgca
      301 ggcaaggccg gcgatccggt gcttgcagca ggtgatggaa aagttatcat cgccaacttc
      361 ctgcgcggct acggcaagct ggtgattgtc aagcacaaca agacctacgt gtcggtctat
      421 ggccataacc gtaccttgct ggtcaaagag ggtcagctgg ttgtcaaggg tcagaagatc
      481 gccgagatgg gtaataccga tgcagatcag gtcaaattgc actttgagat tcggagcaag
      541 ggcaagccgc ttgatccgct caagttcctt ccacagcgat aatcatgccg ggcaagcctg
      601 actccgattc cggcttcgcc gaggacgaga taccgcagga gagaggtccg gcggaggaag
      661 gatttctggc tgaatccgaa gacgaggcaa atactgtgac gccggaagtc gaattgctca
      721 acgacgttac ccagcattac ctcaacgaaa tcggtgccag gccattgttc acaccagaag
      781 aagaatttga ctgggcgtgt cgcgcgcatg gtggagagtt tgttgcaaga caaaagatga
      841 tcgagcataa cttgcggctc gttgtgaata tcgctaagca ctacctcaat cgtggaattc
      901 ctttgcttga cttgatcgaa gaaggaaatc ttggcctcat tcatgctatt gagaaatttg
      961 acccgtggcg tgggttccgt ttctcgacat atgccacctg gtggattcgt cagagtatcg
     1021 agcgagcgat catgaaccag tcacgtacca ttcgtctccc ggtgcatgtg gtcaaggaga
     1081 tcaatcttgt tttgcgagct ctcaggcact tggaattggc gaacggaagg gagattcacc
     1141 tggaacaggt tgcccacctg attgaccggc cgattgaaga ggtgcgcagg gcgctcgcac
     1201 tcaatgagca tatttcatcg cttgatgcac cattggagat tgatcccaac catacggtcg
     1261 ccgatgccat ctctgatgac aatgtactta gcccggaaag tatgcttcaa tccagcgaag
     1321 tgtgcaatct tgtgcgtgaa tgggtcgaac atttgcccga gaagcagcgt cgggttcttg
     1381 agcgtcgcta cggcctgtgg ggtggagaaa tcagtacact tgaggaggtt gccgcggatc
     1441 tcaacctgac tcgcgagcgc gtacgacaaa tccagatcga agcgcttgat cagcttcgtc
     1501 gcgtcataaa gcgcggaggg atgacgcggg aaaacctgct ttgatgcaga gaaggtttca
     1561 tcgaaggtgg ggcgatttgt cctgaagcgg tgctggtgta cctcgaacac aaagaagaat
     1621 cttcatgaac ttgaggatga atcctcgttc cgcagtactc tccatgttct cggggccaac
     1681 cgaggttcag ggttagatct gactacattg atcgattaag gatgtttgat atgggcnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggca ttcatgcgca caatgactgc
     1861 ggcatggctg tggccaatag cgtgactgca gtcgagaacg gtgcccggca tgttcaaggt
     1921 acgtatatcg gttttggcga gcgttgcgga aatgccaacc tgtctaccat aatcgccaat
     1981 ctgggagtga agcgcgactt tgagtgtctt ccggacaacc aatacaggaa cattacccgc
     2041 gtggcgcgtc acattgcagc catctcgaac cttgctctga acgagcgtga cccctatgtg
     2101 ggcaactgtg ccttcgcgca caagggcggc atgcatatcg acggtgtgaa caaggcgtcc
     2161 cattccttcg agcacatcaa cccggatctg gtcggaggtc agcgtcgctt cctgatgagc
     2221 gaagtctccg ggcgacggat gattcttgaa aagatctggg aggtggacgc gagcattacc
     2281 aaggacgatg cggtgaccga cagcatcatc aagcgcctga aggagttgga gcatgagggc
     2341 tatcagtttg agggcgccga aagcacgttc gaacttgtca tacgcaaaca actcggcaag
     2401 tatcgcccgt tctttgagct tgagcatttc aagatcattg gcgagcaacc gacgaacggc
     2461 gaatacagtt cttcagccat cataaaggtg agagtggatg gtactgatga aataaccgca
     2521 gcagaaggcg acggcccggt ccatgcactt gactgcgcct tacgcaaggc tctggacaag
     2581 tttttccccg agctttcgaa cgttcatctt accgattaca aggtgcgtgt catcaacggc
     2641 cgggatgcga cagccgccaa ggtacgtgta ttgctgcaat cgacagacgg cgatgccgtg
     2701 tggacgacag tgggcgtttc cactgatatt atcgaagcca gctggatagc ccttgttgac
     2761 tcgatcgagt acaagttgct agtactggaa gaagccaagt gtccgatgtg aagaagtgac
     2821 aacgtacttt ttgctggtgc ggcacttcga ccgaagttcc agttcacacg aaagccgcga
     2881 acgccgggcc cgtgagtctg gctcgtgaaa tacctgctgg cttgatctgt cccggtaccc
     2941 ggtatctaaa cccatagatt tcgctatgtt cgatacgccc cgtttcaccg attgctttga
     3001 ttttcaccct cgccatggct acggctgtga ctgcggcttg cttcgcgcat tcggcgcgtg
     3061 gatacgcctc tcggcatgca cacaaacgca tg
//
LOCUS       DAORPM010000273         3062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10979_length_3062_cov_6.194879, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000273 DAORPM010000000
VERSION     DAORPM010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10979_length_3062_cov_6.194879"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLD53_10975"
     CDS             complement(<1..509)
                     /locus_tag="PLD53_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRC61483.1"
                     /translation="MTTSIPGFSAPTVGTEAPLEMLSACHGRIETQCVTLRRLVPHLL
                     VHGVDEEARAAATRVIRYFDTSALHHHADEEDDLFPALIESMAGSDAICLRAVIDGLK
                     VDHRALEACWRHLRGILERIAAGEQVSLVADDVEALAVRYACHIEYEEKELLPMAARL
                     LSDHDLARIG"
     gene            complement(506..967)
                     /locus_tag="PLD53_10980"
     CDS             complement(506..967)
                     /locus_tag="PLD53_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRC61484.1"
                     /translation="MQATAACADTDSDLYQAIVDQAPDVIIFADCEGAIRVWNHGAET
                     VFGHSAAEVLGESLDVIIPERLRKAHWEGFHKAVDSGQTKYRNQVLTTRSVHKDGSKL
                     YVELSFGLVRDGSGTIIGSLAIGRDCTARHLKAVAQSSAAAQDKPSDRALK"
     gene            complement(1019..1423)
                     /locus_tag="PLD53_10985"
     CDS             complement(1019..1423)
                     /locus_tag="PLD53_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRC61485.1"
                     /translation="MSKVDFETLAHFRYQLRRYLRFSEDLTQRAGITPLQYLLMLQIK
                     GFPGRESANVGELAERLQAKHHGVVSLISRCEAAGLVQRSVSSSDRRQVDVQLTRKGN
                     ECVEQLAGLHRRELLSLQGTFTVPDLTTLGRK"
     gene            1596..2786
                     /locus_tag="PLD53_10990"
     CDS             1596..2786
                     /locus_tag="PLD53_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC61486.1"
                     /translation="MTIDPHTHGLSTLCIHAGTHLDQATGGACSPIFTSTAYAFPNPA
                     NANIYPRYFNTPNQQVINRKLAALEKGEGALVFGSGMAAISTLLLANLKPGDHAIFQN
                     DLYGGSMRLISSELPRLGVQLSWGANVADFAAALRPETRLIYVESPSNPLLHCVDLAE
                     MAALAKRHGVLTVIDNTFATPINQNPLPLGIDAVIHSATKYLNGHSDVNAGVVVASAE
                     IVSRVTGSAVNFGGMLDAHACSQLERGLKTLALRVQRHNENAETLARFLASHPAVSKV
                     NYPGLPEHPDHAIAARQMRGFGGMLSFELRQPEQLDRLLSRLRIVMPALSLGGVESLI
                     CVPSRSTHRTMTPAERRRAGISDGLLRVSAGIEDVQDLIDDFTDALAGDDRRSPASTR
                     ESPL"
     gene            2783..>3062
                     /locus_tag="PLD53_10995"
     CDS             2783..>3062
                     /locus_tag="PLD53_10995"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRC61487.1"
                     /translation="MSSTDNTQPAPLMIVGEVASSGLARGPAMLCDCARTQTAVPLRQ
                     VSAAEVQAEVERFDAAVLAAEGKLREVQESVRRTLGKDEAEIFEAQVLL"
BASE COUNT          571 a          959 c          920 g          612 t
ORIGIN      
        1 ccgatgcggg ccagatcatg gtcgctcaac aagcgcgcag ccatgggcag gagctccttt
       61 tcctcgtact cgatatggca cgcatagcga acggccagcg cttcgacatc gtcagcgacc
      121 agtgacacct gctcgccggc ggcgatcctt tcgaggatgc cgcgcaggtg tcgccaacac
      181 gcttcgagcg cgcggtgatc gactttcagc ccgtcgatca ccgcgcgcag gcagatggcg
      241 tcagaaccgg ccatcgactc gatcaacgcc ggaaagaggt cgtcttcttc atcggcatga
      301 tggtgcaggg cggatgtgtc gaagtagcgt attaccctgg tcgccgccgc acgcgcttct
      361 tcgtcgacgc catgaaccag caggtgtgga accaggcgcc gcaaggtcac gcattgcgtt
      421 tcaattctgc catggcaggc ggaaagcatc tccagaggcg cctcggttcc gacggtcggt
      481 gcggagaatc caggaatgct tgtcgtcact tcaaggccct gtcgctcggc ttgtcttgcg
      541 cggcagccga agactgtgcg actgccttga ggtggcgggc ggtacagtcg cggccgatgg
      601 ccagggaacc gatgatcgtg ccgctgccgt ccctcaccag gccgaagctc agttccacgt
      661 agagcttgct cccatccttg tgcaccgagc gggttgtgag tacctggttc cggtacttgg
      721 tctggccgct gtcaaccgct ttgtgaaagc cttcccagtg tgctttccgg agccgttccg
      781 gaatgatcac gtcgaggctt tcgccgagga cttcggcggc ggaatggccg aacacggttt
      841 cagcgccatg attccagacg cggatggctc cctcgcaatc ggcgaaaatg atcacatccg
      901 gggcctgatc cacgatcgcc tgatagagat cggaatctgt atccgcacac gcggcagttg
      961 cctgcatcct ggaactcctt tccacttggt cgaatgagac aagcgctcgc cgtcgttgct
     1021 atttgcggcc gagcgtggtt aggtcgggca ccgtgaatgt tccctgcaag gacaggagct
     1081 cacggcgatg aaggccagcc agttgctcaa cgcattcgtt gccttttctt gtcagttgca
     1141 cgtccacctg ccgccggtcg ctgctgctga cgctacgctg tactagtccg gccgcctcgc
     1201 atcgagaaat cagcgacacg accccatgat gcttcgcctg caggcgttcg gcgagttcgc
     1261 cgacgttggc cgattcccgt ccgggaaagc ctttgatctg gagcatcaac agatactgca
     1321 agggggttat cccggcccgc tgcgtcagat cttcgctgaa gcgcaggtag cggcgcagct
     1381 ggtagcggaa gtgggcaagc gtttcgaaat cgaccttgga catttgctgc tcggacattt
     1441 ctgcagggaa ccttcccgta agtttgtatc acagtatgat atggttggct ggatgcaatg
     1501 gcaagttgcc tgggcgcggt gtttcgcaaa cttctcctgg caataaagtc cttatcccga
     1561 ggtatccaca ggcaaagacg actcaactaa acccgatgac tatcgaccca catacccacg
     1621 gcctgtcaac actctgcatt cacgccggca cgcatctgga ccaggccacc ggcggcgcgt
     1681 gcagccccat cttcacttcc acggcctacg cgttcccgaa tccggccaac gcgaacatct
     1741 acccgcgtta cttcaatacg cccaaccagc aggtcatcaa ccgcaagctg gcagcgctcg
     1801 aaaagggcga gggggcgctt gtcttcggct cgggcatggc ggcgatctcc accttgctcc
     1861 ttgctaatct caaacctggc gatcacgcca tcttccagaa cgatctctac ggcggcagca
     1921 tgcggctgat cagcagcgaa ctgccgcggc tcggcgtgca gctttcctgg ggggcgaacg
     1981 tggcggactt cgccgctgcc ttgcgaccgg agacgcggct gatctacgtc gaatctccgt
     2041 cgaatccgct gctgcattgc gttgatctgg ccgaaatggc tgcgcttgcg aaacgccacg
     2101 gcgttttgac cgtcatcgac aacacttttg ccacgccgat caatcagaac ccgcttccgc
     2161 tgggtatcga tgcggtcatc cacagtgcta ccaagtacct caacggccac agcgacgtaa
     2221 acgcgggcgt ggtcgttgcc tccgctgaaa tcgtcagccg cgtgacgggg agtgccgtca
     2281 acttcggcgg aatgctcgat gcgcacgcct gttctcaact ggaacgtgga ctgaagacac
     2341 tggccctgcg cgtgcaacgg cacaatgaga atgccgagac gctggcacgt ttccttgcct
     2401 cgcatccggc ggtgtccaaa gtcaattatc ccggcctgcc cgaacatccc gaccacgcca
     2461 tcgcggcacg ccagatgcgc gggtttggcg gcatgctgtc gttcgagctg cgtcaaccag
     2521 agcagctgga tcgcttgttg agccggctgc gtatcgtcat gccggccctg agtctgggcg
     2581 gcgtggaaag cctgatttgc gttcccagcc gcagtacaca ccgcaccatg acgcctgccg
     2641 agcgacggcg tgccggcatc agcgatggtc tgttacgcgt ctctgcaggc atcgaggatg
     2701 tgcaagacct gatcgacgac ttcaccgacg cactggccgg ggatgatcgt cgttccccgg
     2761 cctccacgag ggaatctccc ttatgagcag cactgacaac acgcagcccg ccccattgat
     2821 gatcgtcggc gaagtggcgt cgtcgggcct tgcccgcgga cccgccatgc tgtgcgattg
     2881 cgcccgaacg cagacagccg ttccgctccg gcaggtgagc gcggcggagg tgcaagctga
     2941 ggtcgagcgt ttcgatgccg cggtgcttgc cgccgagggc aagctgcggg aagttcagga
     3001 gagcgtccgg cggactttgg ggaaggacga ggcggagatt ttcgaagcgc aagtccttct
     3061 cc
//
LOCUS       DAORPM010000274         3061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_10983_length_3061_cov_9.607452, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000274 DAORPM010000000
VERSION     DAORPM010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10983_length_3061_cov_9.607452"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLD53_11000"
     CDS             <1..261
                     /locus_tag="PLD53_11000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRC61488.1"
                     /translation="RFGGEEFIIILPDTTLEDAKTAIVRLQRELTKRYFLHENEKVLI
                     TFSAGVTDHRSDDTQASVTKRADDAMFAAKKAGKNRVVTGNN"
     gene            complement(347..>1174)
                     /locus_tag="PLD53_11005"
     CDS             complement(347..>1174)
                     /locus_tag="PLD53_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61489.1"
                     /translation="RHSLSRGGWYRLGGVLDKDGNRISDSLENWAENALDERGGDFGQ
                     LTDDFADQQLYATRLMGQTHYLVAAAGDGVADFLQLEIEDLQELRVHRLFANDPVSIE
                     ELVDPRGGNDKPVPVGLPFHTFHRIQHIGALLRRMLAQKPEPAPIHRMLEDWSKSSAS
                     SASAYCNHWVIATREHLDHYHQPVFRAQPISTQFDDAPEFDAVAGSSGLELCTALARF
                     DREIGYPMAWYFHMLSSKAVPHWVADCVIEDSLSGYGYLPQCDVNVVRGWLHRPYSV"
     assembly_gap    1175..1274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1275..1498)
                     /locus_tag="PLD53_11010"
     CDS             complement(<1275..1498)
                     /locus_tag="PLD53_11010"
                     /inference="COORDINATES: protein motif:HMM:NF040002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6129 family protein"
                     /protein_id="HRC61490.1"
                     /translation="MITSLQLLQAAEIIQLSSRIDLLRERFPQLHFTECSEDDVSPRH
                     QPALSFDNHDLYLVTGATGECLKLTDDFNKA"
     gene            complement(1495..2226)
                     /locus_tag="PLD53_11015"
     CDS             complement(1495..2226)
                     /locus_tag="PLD53_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     N-terminal domain-containing protein"
                     /protein_id="HRC61491.1"
                     /translation="MPATPIASREAALRIALAARALNGLDVQALVGALVEKLEAPLTE
                     SKLGTLTVDDIRVILAGDFAEQGCHVGVEDAALKDAVRLLWGQGVDSSDFPVVDSSPG
                     DDIPDSIRVAIAANRGENLDGHFGSCERFLIYLVSLTTQKLIDVRSTVEADTTEDRNA
                     ARSALIGDCQIAFVQSIGGPAAAKLVRAGVHPIKKPKGGPAHEVLVELQARLASPPPW
                     LARIMGVPAGSLKRFEEHVQAEEPA"
     gene            complement(2245..2436)
                     /locus_tag="PLD53_11020"
     CDS             complement(2245..2436)
                     /locus_tag="PLD53_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRC61492.1"
                     /translation="MAMKIDPDMCTACGDCKPVCPTKSISSGKVYFKIDVGTCTECEG
                     ESDSPMCVDVCPSGCIVPA"
     gene            complement(2471..2698)
                     /gene="nifT"
                     /locus_tag="PLD53_11025"
     CDS             complement(2471..2698)
                     /gene="nifT"
                     /locus_tag="PLD53_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HRC61493.1"
                     /translation="MANIMIRQADNGGLTFYLPKRDLEASISSIEFDTQEKWGGELKL
                     DNGGSYYIDPIAKPPRLPISLRARRTGMAED"
     gene            complement(2757..>3061)
                     /locus_tag="PLD53_11030"
     CDS             complement(2757..>3061)
                     /locus_tag="PLD53_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HRC61494.1"
                     /translation="GYDLWHMRSLCFTDKPDFLIGNSYGKFIQRDTRFKGEEFEVPLI
                     RLGFPIFDRHHLHRHTTMGYEGMISIVTQLTNAVLEQLDKETMGMATTDYNFDLVR"
BASE COUNT          598 a          839 c          931 g          593 t
ORIGIN      
        1 cgttttggcg gagaagaatt catcatcatc ctgccggaca caacgcttga ggacgcaaaa
       61 acggccatcg tccgtctgca aagggaactc accaaacgct acttcctgca tgaaaacgaa
      121 aaagtgctga tcaccttcag cgcgggagtg acagaccacc gcagcgacga cacccaggcg
      181 agcgttacca aacgggcaga tgatgcaatg tttgcagcta agaaagccgg caagaaccgg
      241 gtcgtcacag ggaataactg agccggcagg aagaggcttg gcgcggtttt gagatctgat
      301 ggatctttgt gccaacccgt ttggacagta cgtcaatagc cgcgatctac accgaatacg
      361 gccgatgcaa ccagccgcgc acgacattga cgtcgcattg cggcaggtag ccgtagccgg
      421 atagcgagtc ctcgattacg caatcggcga cccagtgtgg gacggccttg gagctgagca
      481 tgtggaagta ccaggccatc gggtagccta tttcacggtc gaaacgggca agcgccgtac
      541 acaattcgag cccgctgctg ccggccactg catcgaattc cggcgcgtca tcgaactgtg
      601 tggagatcgg ttgcgcgcgg aatacaggct ggtgatagtg atcgaggtgt tcgcgggtgg
      661 cgatcaccca atggttgcag taggcactgg cactgctggc gctgcttttc gaccagtctt
      721 caagcatgcg gtggatgggc gcgggttcgg gcttttgcgc cagcatgcgg cgaagcaggg
      781 cgccgatatg ctggatgcgg tgaaaggtgt ggaagggcag gccgaccggg acaggcttgt
      841 cattgccgcc tcgcggatcg accagctcct cgatgctgac agggtcgttg gcgaacagtc
      901 gatgtacgcg caactcctgt agatcctcga tctccagttg cagaaaatcg gccacaccat
      961 cgccggcagc ggcgaccaga tagtgcgtct ggcccatcaa gcgagttgcg taaagctgtt
     1021 gatcagcgaa gtcgtcggta agctggccaa aatcaccgcc gcgctcgtcg agggcgtttt
     1081 cagcccaatt ttctaggctg tcggaaattc gatttccgtc tttgtcgagg acaccgccca
     1141 gacgatacca gccgccgcgg ctcaaggaat ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnngctttg ttgaaatcgt cagtcagctt caggcattca cccgtagcgc
     1321 cggtgaccag gtacagatcg tggttgtcga agctgagcgc cggctggtga cgaggtgaaa
     1381 catcgtcttc gctgcactcg gtaaagtgca gttgcggaaa tcgctcgcgc aacaagtcaa
     1441 tgcgcgaact cagttgaatg atctcggccg cctgcaagag ctgaagggac gtgatcacgc
     1501 gggctcctcg gcctggacgt gctcctcgaa gcgtttcaac gagccggccg gcacgcccat
     1561 gattcgtgcc agccagggcg gtgggctggc cagacgggcc tgcagctcaa ccagcacttc
     1621 gtgggcgggg ccgcctttcg gcttcttgat cgggtgtacc ccggcacgca ccagcttggc
     1681 ggcggccggg ccgccgatcg attgcacaaa ggcgatctgg cagtcgccga tcagcgccga
     1741 gcgggccgca tttctgtctt cggtggtatc cgcttcgacg gtggagcgca cgtcgatcag
     1801 cttctgtgtg gtcaggctga ccaggtaaat caggaagcgc tcgcatgaac cgaagtggcc
     1861 gtccaggttt tcgccccgat tggcggcaat ggcgacgcgg atgctgtcgg gaatatcgtc
     1921 gccgggggag ctatcgacca cggggaaatc gctgctgtcg acaccctgcc cccagagcag
     1981 gcgcacggcg tccttgagcg ccgcgtcttc aacgccgaca tggcaaccct gctcggcgaa
     2041 atcgccggcg aggatgaccc ggatatcatc aacggtcagc gtgcccagct tgctttcggt
     2101 caatggcgct tccagttttt ccacgagggc gccaaccaaa gcctggacgt caagaccgtt
     2161 cagcgcgcga gcggccaggg cgatgcgcaa ggccgcttca cgagaggcga tcggtgtggc
     2221 cggcatgaca gtgatcctcc gctgtcaagc cgggacgatg cagccggacg ggcaaacatc
     2281 gacgcacatc ggcgaatcgc tttcgccttc gcattccgtg caggtgccga cgtcgatctt
     2341 gaaatagacc ttgccggaac tgatcgattt ggttgggcaa accggcttgc agtcgccgca
     2401 ggccgtgcac atgtcagggt caattttcat tgccatgata tttctcctgg ttgcttgtct
     2461 aggtgttgac ttaatcctcg gccatgccgg tgcgcctggc ccgtaaggaa atgggcagac
     2521 gcggcggttt ggcgatcgga tcgatgtagt acgaaccgcc gttgtccagt ttcagttcgc
     2581 cgccccattt ttcctgggtg tcgaattcaa tggacgaaat ggatgcttcc agatcccgct
     2641 tcggaaggta gaacgtcagg ccgccgttgt cggcttgacg gatcatgata ttggccatca
     2701 aatgctcctg cagtaccggc ggcgcccgag ttgccaggcg ccgccagcgg ttcggattaa
     2761 cgaaccaggt cgaagttgta gtccgtagtc gccatgccca tcgtctcctt gtcgagttgt
     2821 tcaaggacgg cgttggtcag ttgggtgacg atcgagatca tgccctcata gcccatcgtc
     2881 gtatggcgat gcaggtggtg acggtcaaag atcgggaagc caaggcggat cagcggcact
     2941 tcgaactctt cgcccttgaa gcgggtatcg cgctggatga acttgccgta ggagttgccg
     3001 atcaggaaat ccggcttgtc cgtgaagcac aggctgcgca tgtgccacag gtcgtaaccg
     3061 g
//
LOCUS       DAORPM010000275         3034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_11155_length_3034_cov_9.354481, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000275 DAORPM010000000
VERSION     DAORPM010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11155_length_3034_cov_9.354481"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2100
                     /gene="plsB"
                     /locus_tag="PLD53_11035"
     CDS             <1..2100
                     /gene="plsB"
                     /locus_tag="PLD53_11035"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HRC61495.1"
                     /translation="VPVVILWGRNPGKQESILKALFAEAWQQPGYLRRLVTILLHGRN
                     VLVRFNAPLSLHALFRDGLDETQALHKLSRVLREHFRRQRLMAIGPDLSHRHTQVGTL
                     LDSAPVRSAIAAEAAEKHIPLDEAQLRARDFALEIASDYSYGVLRALELSLAWFWKRL
                     YDGIELHNTDVVARIAPNHGIVYIPTHRSHIDYLLLSYFLHRQGLAPPHIAAGANLNI
                     PLIGPLLRRAGAFFMRRSFRGEPLYAAVFHEYLHLMLTRGFPIEYFIEGGRSRNGRML
                     TPRAGILGMTIRSFIREHSRPLVFVPVYIGYEKLVEGQSYLREMAGKPKQSESLWSLL
                     KSLRRIKRIFGKAYVNFGEPLALADFLDAYRPGWSNEAATDSDDWAREATHITASELA
                     RRMNEAAVINPVNLIALVLLPTPVLPTDEQSLGRLLEHFQSLIADAPYSRATVPCTLD
                     AEQIVAYVERLGIVERISHPQGDLIRLVEDESPLLAYFSNNVLHLFTLPALIACLMSH
                     NKPLGMQQIIDAVAGIYGLLEKELFLRWTIDEIPAATETVIGVLLKRSLLLRDEAGQL
                     AAPDQGTRECAELRAIGETIRPLLDCNFLTLSLLQQGGSGRRTRRSLENDCHRLARRL
                     ILRYETNAPEYSNKTGFSTLIDQLVDANLLREDEAGHLLPDRHIATLLAHAMPFIPPE
                     TRQVIEGMACADPEVLR"
     gene            complement(2097..2786)
                     /locus_tag="PLD53_11040"
     CDS             complement(2097..2786)
                     /locus_tag="PLD53_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRC61496.1"
                     /translation="MSATITLKQSMARSSDVVLKIVQYSLMIAGLFLVLGFFGNFSKS
                     TLSAGLLKADPSELSAADETSEVVAESEAAAPALSPGMQGALDYVTRRYRVSQEALEP
                     IFGVAQMIGKERRIDPLLIVAMIGIESGFNPFAESPMGAQGLMQVIPRFHMDKVPDGA
                     GDKPFLDPETNIRVGVHVLQEAIRRRGGLVAGLQYYAGSSDPEGSYASKVLAEQERLK
                     NAVRRSSRSNA"
BASE COUNT          640 a          911 c          845 g          638 t
ORIGIN      
        1 gtcccggtcg tcatcctttg ggggcgcaac ccaggcaaac aggagtcgat cctaaaagca
       61 ctgtttgccg aggcctggca acaaccgggc tatctgcgcc gtctggtgac catactgttg
      121 catggccgga acgtactggt ccgcttcaac gcaccgcttt ccctgcacgc cctgttccgc
      181 gatggcctcg acgaaacaca ggccctccac aagttgtccc gcgtgctgcg tgagcacttc
      241 cgccgccagc gactgatggc catcgggcca gacctttcgc accgccatac gcaagtggga
      301 accctgctcg actccgcgcc agtacgctcg gcaattgccg ccgaggcagc ggaaaaacat
      361 attccacttg atgaagccca actgagggca cgcgatttcg cgctggaaat cgcctcggat
      421 tactcgtatg gcgtgttgcg tgccctcgag ttgtcgcttg cgtggttttg gaaacggctt
      481 tatgacggca tcgagttgca caataccgat gtcgttgcgc ggattgcgcc caaccacggc
      541 attgtctaca taccgacgca tcgcagccat atcgactatc tgctgctttc gtactttctg
      601 caccgacaag ggctggcccc gcctcacatc gccgccggcg ccaatctgaa cattcccctc
      661 atcggtccat tgctccgccg ggcaggcgct ttcttcatgc gccgcagctt cagaggcgaa
      721 cccctctatg cggcagtttt ccatgaatat ctgcatctga tgctgacccg cggctttccg
      781 atcgagtact tcatcgaagg ggggcgaagt cgcaatggaa gaatgttgac gcccagggcc
      841 ggcatcctgg gaatgacgat tcgcagtttc atccgggagc attcacgacc gctcgttttc
      901 gtgccggtct atatcggcta cgagaaactc gttgaaggtc aatcctacct tcgtgaaatg
      961 gccggcaagc cgaagcaaag cgagtcactg tggtcgctgc tcaaaagcct gcgccgaatc
     1021 aagcgaatat tcggcaaagc gtatgtgaac ttcggcgaac cgctggcgct ggccgacttt
     1081 ctggacgcct atcgccccgg ctggtcaaac gaagcggcga ctgactccga cgactgggcg
     1141 cgcgaggcaa ctcatatcac tgccagcgaa ctggccaggc gcatgaacga agcggcagtg
     1201 atcaatccgg tcaacctgat cgctctggtg ctgttgccga cgccagtgct gcctaccgat
     1261 gagcaatcac tggggcggct tctggagcat ttccagtcgc taatcgccga tgcgccttat
     1321 tctcgggcta ccgttccatg cactcttgat gccgagcaga ttgtggccta tgtcgaacgg
     1381 cttggaattg tcgagcgcat ttcgcacccg cagggcgatc tcattcgtct ggtggaagac
     1441 gagtctccgc tgctcgctta ctttagcaac aacgtgctgc atctgtttac cttgccagca
     1501 ctgatcgcct gtctgatgag ccacaacaag cccctgggca tgcaacagat cattgatgcc
     1561 gttgccggta tctacggcct gctggaaaag gaacttttct tgcgctggac gatagatgaa
     1621 attccggcag ccaccgaaac cgtcattggc gtactgctca aacggagcct gctactccgc
     1681 gatgaagcag gtcaactcgc agccccggat caaggcactc gggagtgcgc cgagttgcgt
     1741 gcaatcggcg aaacaattcg accgctactc gactgcaact tcctcacgct gtcattgctg
     1801 caacagggcg gatcaggtcg ccggacacgt cgctctctgg aaaacgactg ccatcggctg
     1861 gctcggcgtt tgatactgcg ctacgagacc aacgcgccag agtattccaa caagactggc
     1921 ttctccacac tcatcgacca acttgtcgat gcgaaccttc tgcgtgagga tgaagccggg
     1981 catttgcttc ctgaccggca catcgccacg ctgctggcgc acgccatgcc gtttatccca
     2041 cccgaaacgc gacaggtaat tgagggtatg gcctgcgcgg acccggaggt gctgcgctag
     2101 gcgttggaac gggaagaacg gcgcaccgca ttcttgaggc gctcctgctc ggccaagacc
     2161 ttgcttgcat agctgccttc aggatccgag gatccggcat agtactgaag accggcaacc
     2221 aacccaccgc gacggcgaat ggcctcctga agaacgtgta cgccgaccct gatattggtc
     2281 tccggatcga gaaagggttt gtcgcccgcg ccatccggca ccttgtccat atgaaagcgc
     2341 ggaataacct gcatcaggcc ctgcgcaccc atcgggcttt cggcaaacgg gttgaaaccc
     2401 gattcaatgc cgatcatcgc cacgatcagc agcggatcga tgcgccgctc ctttccgatc
     2461 atttgcgcca ctccaaaaat cggctccagc gcttcctggg agacacgata gcggcgtgtc
     2521 acataatcca gcgcgccttg catgcctggc gacagagcgg gcgccgcagc ttcggattct
     2581 gcaaccactt ccgaggtttc atccgcagcg gacaattcgg acgggtcggc tttgaggagc
     2641 ccagccgaca gggtgctttt ggaaaaattg ccgaaaaagc caaggacgag aaagaggccg
     2701 gcaatcatca gactgtattg aactattttc aggacgacat cgctactgcg tgccatcgat
     2761 tgtttgagcg taatcgttgc agacatgctc actctccttc actgttgtcg tcgcgtaagc
     2821 aaacaggccg ggcgacaaac ggttctaggg gggtgtaagt cccgtgaatg cggtggtgtt
     2881 taccgcaagg tgggcacttc tatgagatgg aaaccctgcg tcaaggttgc agggggagaa
     2941 gtgtccagct ggcacaaatc acgcgcttgg cgtttcgtgc agcgggcgga aggtatcatt
     3001 taggtaaatg cttgtcaacc ataaatagac agct
//
LOCUS       DAORPM010000276         3030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_11182_length_3030_cov_13.421513, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000276 DAORPM010000000
VERSION     DAORPM010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11182_length_3030_cov_13.421513"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLD53_11045"
     CDS             complement(<1..214)
                     /locus_tag="PLD53_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRC61497.1"
                     /translation="MAMLELLSEHACFGGIQRFYQHQSTEIGLPMRFSVYLPPRAAQD
                     SVPAVMYLAGLTCTEETFMIKAGAQRI"
     gene            complement(214..1320)
                     /locus_tag="PLD53_11050"
     CDS             complement(214..1320)
                     /locus_tag="PLD53_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HRC61498.1"
                     /translation="MKTKAAIAWKSGSPLTIETVDLQGPQSGEVLVEIKATGICHTDY
                     YTLSGADPEGIFPAILGHEGAGVVVEVGPGVTTLQKGDHVIPLYTPECRQCKFCLSRK
                     TNLCQSIRGTQGKGLMPDGTSRFSLDGKPIFHYMGTSTFSNYTVAPEISLAKIRKDAP
                     FDKVCYIGCGVTTGIGAVLFTARVEAGANVAVFGLGGIGLNVIQGARMVGADKIIGID
                     INPARQDIARKFGMTHFINPKDTENVVDAIVQLTDGGADYSFECIGNTQVMRQALECT
                     HKGWGRSIIIGVAEAGAEISTRPFQLVTGRKWEGTAFGGARGRTDVPRIVDWYMEGKI
                     NIDDLITHTMPLEDINKGFDLMKSGDSIRGVVLY"
     gene            complement(1471..2580)
                     /locus_tag="PLD53_11055"
     CDS             complement(1471..2580)
                     /locus_tag="PLD53_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HRC61499.1"
                     /translation="MRIIRFLIGLGFALISMTSTAAELPLEKIKLPPGFTIELWARVD
                     NARQMALGRHDATGGTLFVGSMQAGKVYAVGFGPDYEARGVTVVASGLQRPVGVAYKD
                     GNLYVSAVSRILRYDGIERRLDHPPAAVVVTDKLPSETHHGWKFIAFGPDGKLYVPIG
                     APCNICEPDPQRYANIMRMNTDGSALEVYAKGVRNSVGFDWQPQSKELWFTDNGRDML
                     GDDLPPDELNHAPRAGMDFGYPYCHGGDLPDPDFGAGQQCARFAAPAQELGAHVAALG
                     MRFYTGTMFPPEYRNQIFVAEHGSWNRSRKSGYRVTLVRLQGSKAVAYETFASGWLQG
                     ETTWGRPADVLVLPDGSLLVSDDYAGAIYRITYRG"
     gene            complement(2626..>3030)
                     /locus_tag="PLD53_11060"
     CDS             complement(2626..>3030)
                     /locus_tag="PLD53_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRC61500.1"
                     /translation="YLGLSAYPEVPQVLRRLKNGGMRLAILSNGTPRMLQSAVSNAGL
                     EGLFDAVFSVEEVGVYKPHPSVYRLAVERLGVEAEQVCFLSSNGWDAYSAKAFGLHVL
                     WCNRFAQVPERIPSQPDAEISDLGVLPEFVID"
BASE COUNT          617 a          912 c          879 g          622 t
ORIGIN      
        1 caatgcgttg cgcaccggcc ttgatcatga aggtttcctc ggtgcatgtc agaccggcca
       61 gatacattac ggccggcacg ctgtcctgtg cggcgcgggg tggaaggtac accgaaaagc
      121 gcatcggcag accgatctcg gtcgactgat gctgatagaa gcgctgtatg ccgccaaagc
      181 aggcatgctc gctcaacaac tccagcatag ccatcagtac aacacgacgc cgcgaatgga
      241 atcgccgctc ttcatcagat caaacccctt gttgatgtcc tccagcggca tggtatgggt
      301 gatcaggtca tcgatgttga tcttgccctc catgtaccag tcaacgattc tggggacgtc
      361 ggtgcggccg cgagcgccgc cgaatgccgt accttcccac ttgcgtccgg tgaccagctg
      421 gaagggccgg gtgctgatct ccgcgccagc ctcggcgacg ccgatgatga tgctgcgtcc
      481 ccagcccttg tgcgtgcatt ccagcgcctg gcgcattacc tgggtgttgc cgatgcattc
      541 gaagctgtag tcggcaccgc catcggtcag ttgcacgatg gcgtccacga cgttttcggt
      601 gtccttgggg ttgatgaagt gcgtcatgcc gaactttcgc gcaatgtcct ggcgggccgg
      661 attgatgtcg ataccgatga tcttgtccgc cccgaccatc cttgcgccct gaatcacgtt
      721 gaggccaatg ccgcccaggc caaataccgc cacgttggca ccggcttcta ccctggcggt
      781 gaacagcact gcgcctattc cggtggtcac gccgcaaccg atgtagcaga ccttgtcgaa
      841 cggggcgtcc ttgcggatct tggccagtga aatttccggg gcgacggtgt aattgctgaa
      901 ggtagaagtg cccatatagt ggaagatggg tttgccgtcc aggctgaaac ggctggtgcc
      961 gtcgggcatc aggcccttgc cctgcgtgcc gcggatgctt tggcacaggt tcgttttgcg
     1021 gctgagacag aatttgcact ggcggcattc cggcgtgtac aaaggaatga catggtcgcc
     1081 tttttgcagc gtggtgacgc ctggccccac ctcgacaacg acccctgcgc cctcgtgccc
     1141 cagaatcgcc gggaaaatgc cttccgggtc ggcacccgag agcgtgtaat agtcggtgtg
     1201 gcaaatgccc gtggccttga tctcgaccag cacttcacca gactgggggc cttgcaggtc
     1261 gacggtttca atggtcagcg gggagcccga tttccaggca attgcagctt tggttttcat
     1321 ggtgaggtta gcgccagtag ggtttcggga aacgattgac ctcgattcga aaaagagagt
     1381 tcattcatcc gaggctcaac ttttccgatc tcgaaatttc gttgatgatt ggactgaaca
     1441 aactgcaacg attcaacggt catgcccggt tcagccgcga taggtgatgc gataaattgc
     1501 tccggcatag tcgtcggaaa ccagcagcga gccatcaggt aaaaccagca cgtcggcggg
     1561 ccgcccccac gtcgtctcgc cctgcagcca gccactggca aaagtttcgt aggccactgc
     1621 cttgctgccc tgcagacgaa ccagcgtcac ccggtagccg ctcttcctgg agcggttcca
     1681 cgagccgtgt tcggcaacga atatctggtt ccgatattcc ggtggaaaca tcgttcccgt
     1741 atagaagcgc atacccaacg cagcgacatg cgcgcccagt tcttgcgccg gggcggcaaa
     1801 ccgtgcgcac tgttgtcccg caccgaagtc cgggtcgggc aggtcgccgc cgtggcaata
     1861 cggataaccg aaatccattc ccgcgcgtgg cgcatgattc agttcgtcgg gtggcagatc
     1921 gtcaccgagc atgtcgcggc cattgtcggt aaaccagagt tccttgctct gcggctgcca
     1981 gtcgaatcca acgctgttgc gcacaccctt ggcatagact tccagagcgc tgccatccgt
     2041 attcatacgc atgatattcg cgtagcgctg cggatcgggt tcgcagatat tgcacggcgc
     2101 gccgatcgga acgtagagtt ttccgtctgg tccgaaagca atgaatttcc aaccgtggtg
     2161 tgtttccgaa ggcagcttgt ccgtgaccac gacggcagca ggcggatggt ccaggcgacg
     2221 ttcgataccg tcgtatcgca gaatgcggct taccgccgac acataaagat tgccgtcctt
     2281 gtatgctacg ccgaccgggc gttgcaggcc gctggctact acggttacac cccgcgcttc
     2341 gtagtccggg ccgaagccaa ccgcatacac cttcccggct tgcatcgatc cgacgaaaag
     2401 cgtaccgcca gttgcatcgt gacgccccag cgccatctgc cgggcgttat ctacgcgcgc
     2461 ccaaagctcg atagtgaaac ccggcggcaa cttgattttc tccagcggca gctctgccgc
     2521 cgtcgaagtc atcgaaatca gcgcaaagcc caagccaatg aggaaacgga ttatgcgcac
     2581 ggtgaccgcg ttacggctcg aagcattacc tgaaaaaggc tgctttcagt caatcacgaa
     2641 ttccggcaag acgcccagat cggaaatctc ggcgtcgggt tgcgagggga tgcgttcggg
     2701 cacctgcgcg aatcgattgc accacagcac atgcaatcca aatgctttgg cggaatacgc
     2761 atcccagcca ttcgacgaca gaaaacagac ctgttcggcc tcaacgccca gacgttcgac
     2821 ggccagacga tagaccgacg gatgcggttt gtagacgcca acttcctcga cggaaaacac
     2881 cgcgtcgaac agtccttcca gaccggcatt ggaaacggcg gattgcagca tgcgcggcgt
     2941 cccgttggac agaatggcca ggcgcattcc gccattcttg agtcggcgca agacttgcgg
     3001 cacttccgga taggcgctca ggccgagata
//
LOCUS       DAORPM010000277         3009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_11346_length_3009_cov_10.767434, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000277 DAORPM010000000
VERSION     DAORPM010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11346_length_3009_cov_10.767434"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLD53_11065"
     CDS             complement(<1..912)
                     /locus_tag="PLD53_11065"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRC61501.1"
                     /translation="MENHDYDLFVIGAGSGGVRAARMAAEFGARVAVAEDRYMGGTCV
                     NVGCVPKKLYVYAAEFGKSFTDARNFGWDSDRSAFNWATLRDNKKAEISRLNGVYDNL
                     LAGVKVEVISGRARIVDAHTVAVGEQRYSAEKILIATGGWPHIPEFPGSEHAISSNEV
                     FDLAQFPRRLAIVGGGYIAVEFAGIFNGLGARVSQLYRGPLFLRGFDADLRAHAAQEI
                     VKTGVDLRFEVDVQSIAAGPGGLQVLLTNGAAIEVDAVLYATGRRANLEGLGLENVNV
                     KISDAGTIEVDEDITPVKADHDMTSGVG"
     gene            complement(1140..1565)
                     /locus_tag="PLD53_11070"
     CDS             complement(1140..1565)
                     /locus_tag="PLD53_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HRC61502.1"
                     /translation="MEPHLTDIARVIQLAVAPVFLLTAIATLISAMNTRLGRIVDRRR
                     VIQTRLKESDQPEARNELAKLARRTRLIYYSIFWAVFAALLVCLVVAGAFLGALIAVN
                     LSRMVATLFIAAMLAIIAALGLFLREIYLAVRAAGHEHL"
     gene            1808..2050
                     /locus_tag="PLD53_11075"
     CDS             1808..2050
                     /locus_tag="PLD53_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61503.1"
                     /translation="MKRESDYADCLAVDQRGLRFVARAMRHGFGGECQGAFWSTTYEG
                     GINAELFAELLKKMMKYRKKPVPSCWIVCRHTRRRY"
     gene            complement(2365..>3009)
                     /locus_tag="PLD53_11080"
     CDS             complement(2365..>3009)
                     /locus_tag="PLD53_11080"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRC61504.1"
                     /translation="PSSAPMSGKLSLQNSTMDVGGNAIIGPQYQGGAGVLELTDSTLR
                     VHGAVMRVGQSSNNGTLFGDGAVSVERSLVDVDNLLRLDVGSSLNFAIDGLARVSEYG
                     AFDVGTAELDGLLNIDLGLALFPLLGVVHFDLIVSSLLDGISGDFDVVNLTGLASGYA
                     ASYGVVVDQGVEIYRLSLTRGQVPEPGTLALLVLALLGMLSMQYRRGLRTVGAR"
BASE COUNT          591 a          933 c          898 g          587 t
ORIGIN      
        1 ccccacaccg ctcgtcatgt cgtggtctgc cttcaccggc gtgatgtcct cgtcgacctc
       61 gatggtgccc gcgtcgctga tcttcacatt cacattctcc agacctaggc cctccaggtt
      121 ggctcggcgt cccgtggcgt aaagcacggc atcgacctcg atggcagcgc cattggtaag
      181 tagtacctgc aggccaccgg gtcccgcggc gatggactgc acatcgacct caaagcgcag
      241 gtctacgccg gtcttgacga tttcctgggc ggcgtgggcg cggaggtcgg cgtcgaagcc
      301 acgcaggaat agagggccac ggtagagctg gcttacccgc gcacccaggc cattgaagat
      361 tccggcaaat tccaccgcga tatagccgcc accgacgatt gccagacgcc ggggaaattg
      421 tgcaaggtcg aacacctcgt tggagctgat ggcatgctca ctaccgggga attccggaat
      481 atgcggccaa ccgccggtgg cgatcaggat cttttctgcc gaatagcgtt gctcgccgac
      541 agcgaccgtg tgggcatcga cgatccgggc gcggccgctt atcacctcga ccttcacccc
      601 cgccagcaga ttgtcataga ctccattgag gcgcgatatt tcagctttct tgttgtcgcg
      661 cagggtcgcc cagttgaaag cggacctatc gctatcccag ccgaagttcc gggcatcggt
      721 gaaggacttg ccgaattcgg ccgcatagac gtacagtttt tttggtacgc accccacgtt
      781 cacgcaagtg cctcccatgt agcggtcttc tgcaactgca accctggccc caaactcggc
      841 agccatgcgg gccgccctga ccccgcccga gccggcgccg atcacaaata ggtcgtagtc
      901 atgattttcc acagattttc ccctcgccat tccgagtcac aagtctatca gcttggcagt
      961 gctgccggtt tggtcgagtt gccgccacag tttcagcggt gccaagacgc ccgctttttc
     1021 gcgatttcaa ttgcgttcca caagctgggc cagcgtctag aaatcggctg agaggcgttt
     1081 gtaataccgg cctttcgtct tcgcgtatgc cgaccgacca agcctgcttc ccatccgggc
     1141 tagaggtgtt cgtgacccgc cgcgcgtacg gcaaggtaga tctcgcgcag gaacaggccg
     1201 agggcggcga tgattgccag cattgccgcg atgaacaatg ttgcaaccat gcgcgacagg
     1261 ttgaccgcga tgagagcgcc aagaaaggct ccggcgacta cgagacagac cagcagcgcc
     1321 gcgaaaacgg cccagaagat cgagtaatag atcagtcggg tgcgccgtgc gagtttcgcc
     1381 agttcgttgc gcgcctcggg ctggtcgctt tcctttaaac gtgtctggat tactcgccgg
     1441 cggtcgacga ttcggcctag ccgagtgttc atggcgctga tcagggtggc gattgcagtc
     1501 agcagaaaga ctggggcaac ggccagctgg atgacgcggg cgatgtctgt gagatgtggc
     1561 tccatcggca ggatgcagtc ctccgcttcg aaggggattt gtatctagtg tgccacggta
     1621 tcttcaccga tggttgagtg acgacgcaag ataaccgcgg ttcgcttcag cttggtctgc
     1681 aaggcaggcg ggtagagtat cgccgtgccc atttttatcc gatttcaccc attcagccca
     1741 tccgcgctgg aaacgccgat cgattgccac tgcctgtgct gcgccttccc tgttgttctt
     1801 ccatcaattg aaacgtgaat ccgactacgc ggactgcctg gccgtcgacc agcgaggttt
     1861 gcgcttcgtg gcgcgtgcca tgcgccatgg cttcggcggc gaatgccaag gagcgttctg
     1921 gtctaccacc tacgagggtg ggatcaacgc ggagttgttc gccgaactgc tcaagaaaat
     1981 gatgaaatac cgcaagaagc ctgtcccctc gtgctggata gtctgccggc acacaagacg
     2041 gcgttactga agcaatacgt cacgcccacc gaaggccgtt cgaccctgca cttcctgccc
     2101 ggctacgccc cgggcttgag tccagacgaa ttggtgtggc gtcatgtcaa acgtaccggc
     2161 accgcgaggc ggccattgaa aaaaggtgag aggctgcgcg acaagtccga ggagcagctt
     2221 gctgcgctcc agaaattgcc actcctggtt cgctcattct tcaacgcccc ttctgtcgcc
     2281 tatattggcg actgccgagt aacgcgtggc aagcgctata agacctcggc caggcacgac
     2341 accggcgcta tgactgggtc ggggtcagcg tgcgccaacc gtacgcaagc cacgacggta
     2401 ttgcatcgac agcatgccaa gcagtgccag caccagcaat gcgagagttc ccggctcggg
     2461 aacctggcca cgggtgagcg acaggcgata gatctcgacg ccctggtcca ccactacgcc
     2521 gtacgaggcg gcgtagcccg acgccaggcc cgtcaggttg accacgtcga agtcaccgct
     2581 gatgccgtcg agcagggacg acacgatcag atcgaaatgc acgacgccaa gcaacggaaa
     2641 cagcgcgagc cccaggtcga tattgagcag tccgtccagc tccgcggtgc cgacatcgaa
     2701 ggcgccgtac tcactcaccc gggcgaggcc gtcgattgcg aaattcaggg acgagccgac
     2761 gtcgaggcgg agcaggttgt cgacatccac caggctacgc tcaacgctga ctgcgccgtc
     2821 gccgaacaac gtaccattgt tggagctttg cccgactcgc atcacggccc catgcacgcg
     2881 cagagtgctg tcggtcagtt cgagcacgcc tgcgccgccc tggtactgcg ggccgatgat
     2941 ggcgttgccg ccgacgtcca tggtgctgtt ctgcagcgag agcttgccgg acatcggtgc
     3001 cgacgaggg
//
LOCUS       DAORPM010000278         2997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_11415_length_2997_cov_12.181849, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000278 DAORPM010000000
VERSION     DAORPM010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11415_length_2997_cov_12.181849"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /gene="rfbC"
                     /locus_tag="PLD53_11085"
     CDS             <1..210
                     /gene="rfbC"
                     /locus_tag="PLD53_11085"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRC61505.1"
                     /translation="RQMWIPPGFAHGFFVLSEYADFLYKATDYYAPEFERGILWNDPN
                     LAITWPLTGTPVVSAKDAQNAPLAI"
     gene            350..2155
                     /locus_tag="PLD53_11090"
     CDS             350..2155
                     /locus_tag="PLD53_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRC61506.1"
                     /translation="MSNALKTPSTVAPATNFIRNIIDADLAAGKHAQRRWSGKPGLGD
                     QQKAGELDAARIRTRFPPEPNGYLHFGHAKSICLNFGLAADYGGRCHLRFDDTNPEKE
                     EKEYVDSIIDSVRWLGFSWQDEVEDNLYYASDYFDWMVACAESLIRSGNAYVDSQSAD
                     EMRAGRGTLTQAGQDSPYRNRSVEESMDLFKRMQAGEFPDGAYILRAKIDMASPNINL
                     RDPAIYRIRHATHHNTGDRWCVYPMYTFAHPIEDALENITHSICTLEFEDQRPFYDWL
                     LERLAEDNLFQRPLPQQIEFSRLNLTYVVLSKRKLIQLVDEKHVAGWDDPRMPTLVGA
                     RRRGYTPEGFRLFAERVGVSKSDSLIEYALLEEAMREVMNESAERRIAVLDPLKLIID
                     NYPEGQSEDCFAPNHPLKPELGKRAMPLGRELWIEREDFMEVPSKGYRRLYPGNSARL
                     RYGFVVQCTGCDKDANGKVIAVHCDYMPDSKSGTPGSDNYKVKGNLHWVSVAHGHAAE
                     VRLYDRLFSTPAPGSRREGDPPEQERDFLDDLNPNSVNVIQAWLEPSLKQVGPEDRFQ
                     FERHGYFVADRFDSKDGAPVFNRTVTLKDSPAKNA"
     gene            2152..2988
                     /gene="pyrF"
                     /locus_tag="PLD53_11095"
     CDS             2152..2988
                     /gene="pyrF"
                     /locus_tag="PLD53_11095"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRC61507.1"
                     /translation="MNFIESLNAAWIKNNSLLCVGLDPDPAKFPAHLKSRPEAIFEFC
                     ARIADATADLVCCFKPQIAYFAAQRAENQLEALIAHIHAKHPGIPVILDAKRGDIGST
                     AEQYAVEIFERYQADAITVNPYMGRDSVEPYLDYPDKGVILLCRTSNPGGSDLQFLNV
                     GMADQPEKLYERVARLVAREWNANGQCALVVGATFPGEIARVREIVGDLPLLVPGIGA
                     QGGDVQATVQAGRTANGCGLMINSSRAILYAGAKDGSETGFTDAARRVALETRDTINR
                     YR"
BASE COUNT          649 a          902 c          841 g          605 t
ORIGIN      
        1 cggcagatgt ggataccacc gggttttgcc cacggatttt ttgtgctcag cgaatacgcg
       61 gattttcttt acaaggcgac tgattattat gcgccggaat tcgaacgcgg cattctctgg
      121 aatgacccga acttggccat cacctggcca ctgacgggaa cgccggtagt ttcggccaag
      181 gatgcacaga acgccccact agctatttga cgctaaaccg tcggcctgaa agcgggctca
      241 tccggggtgc cctcttggcc gagatttgaa gattccatcc tgccacggca acttgcggtg
      301 gcaggctcag cggttaaaat acggctcttc tggaatcaag gaccacgacg tgagcaacgc
      361 cctgaaaacc ccttccaccg tagcgcctgc gaccaatttc attcgcaaca tcattgatgc
      421 tgatctggcg gccggcaagc acgctcaacg acgctggtcc ggcaaacccg ggcttggcga
      481 tcagcagaaa gctggcgagc ttgatgcggc aagaattcgt acccgctttc cgccggagcc
      541 gaacggctat cttcatttcg gccatgccaa atcgatctgc ctgaacttcg gcctggcggc
      601 ggactacggt ggccgctgtc acttgcgttt tgacgacacc aatccggaga aggaagaaaa
      661 ggaatacgtc gattcaatca tcgactcggt gcgttggctt ggtttttcct ggcaggacga
      721 ggtcgaggac aatctgtact acgcttccga ctacttcgac tggatggtgg cctgcgccga
      781 atccttgatt cgatcgggaa acgcctacgt cgacagccag agcgccgacg agatgcgcgc
      841 cggtcgcggc acgctgaccc aggccggcca ggattcgccg taccgcaatc gcagcgtcga
      901 agaaagcatg gatctcttca aacgcatgca ggctggcgag tttccggatg gggcgtacat
      961 cctgcgagcg aaaatcgata tggcctcgcc caatatcaac ctgcgcgacc cggcgatcta
     1021 tcgcattcgt catgcgacgc accacaatac gggcgacagg tggtgcgtct acccgatgta
     1081 cactttcgcg catccaatcg aagacgcgct ggaaaacatc actcattcga tctgcaccct
     1141 ggagttcgag gatcagcggc ctttctacga ctggcttctg gagcgacttg cggaagacaa
     1201 tctgtttcag cggccgctcc cacaacagat cgaattttcg cgactgaacc tgacctacgt
     1261 cgtgctatca aagcgcaagc tgatccagct cgtcgatgaa aaacacgtag ccggttggga
     1321 cgatccgcgc atgccgacgc tggtcggtgc gcgtcggcgt ggttataccc cggagggttt
     1381 caggctcttt gccgaacgcg ttggcgtatc caaatcggat tcgctgatcg aatacgcgct
     1441 gctggaagaa gccatgcgcg aagtaatgaa tgaatccgcc gagcgtcgca tcgccgtgct
     1501 cgaccccttg aaactgatca tcgacaatta cccggaagga caaagcgagg attgcttcgc
     1561 gcccaaccac ccgctcaaac cggaactcgg caaacgcgcc atgcctctgg gtcgcgaatt
     1621 gtggatcgag cgcgaggact tcatggaggt gccgagcaag ggctaccgca ggctgtatcc
     1681 gggaaacagc gcccgcttgc gctatggctt tgtggtccag tgcaccggct gcgacaagga
     1741 cgcgaacggc aaggtcatcg ccgtccattg cgactatatg cccgactcga agagcggaac
     1801 tccaggttct gataactaca aggtcaaggg caatctgcac tgggtctcgg tggcgcatgg
     1861 ccatgccgcc gaagtacggc tttacgatcg ccttttttcc acaccggcgc ccggcagccg
     1921 gcgcgaaggc gatccgccag agcaggagcg cgactttctt gacgatctca acccgaacag
     1981 cgtgaacgtc atccaggcct ggcttgaacc gtcgctcaag caagtcggac cggaagatcg
     2041 tttccagttc gaacggcatg gttacttcgt cgctgatcgc ttcgactcga aggatggcgc
     2101 accggtattc aaccgcacgg tgacattgaa ggattcgccg gcgaaaaacg catgaacttt
     2161 atcgaatcgc tcaacgctgc ctggatcaag aacaattcat tactctgtgt cggcctggac
     2221 cccgacccgg cgaaatttcc ggcacatctc aagagtcggc cggaagcgat tttcgagttt
     2281 tgcgctcgca ttgccgacgc caccgccgac cttgtctgct gcttcaagcc acaaattgct
     2341 tactttgctg cgcagcgtgc cgaaaaccag ctcgaagcgc tgatcgctca tatccatgcc
     2401 aagcatcccg gtatcccggt catactcgat gccaagcgcg gcgacattgg cagtaccgcc
     2461 gaacagtatg ccgtcgaaat tttcgagcgc taccaggctg acgccatcac ggttaatccg
     2521 tacatggggc gcgactcggt cgagccgtat cttgattacc cggacaaggg tgtaatcctg
     2581 ctctgccgca catccaatcc cggtggcagt gacctgcaat tcctcaatgt cggaatggcc
     2641 gatcaacccg aaaaacttta tgagcgcgtg gcccgactcg tggcacgcga atggaacgcc
     2701 aatggacaat gcgcgctggt tgtcggcgct actttccccg gcgaaattgc ccgagtgcgt
     2761 gagatcgtcg gcgatctgcc gctgcttgtt cccggcatcg gtgcgcaggg cggtgatgtt
     2821 caagccaccg tacaggcagg ccgcaccgcc aacggctgcg gcctgatgat caactcatcg
     2881 cgcgccatcc tctacgccgg tgccaaggat ggttcggaaa cgggcttcac cgatgccgca
     2941 cgccgtgttg ctctggaaac ccgcgatacg atcaaccgct atcgctgatc agccagg
//
LOCUS       DAORPM010000279         2963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_11622_length_2963_cov_13.183975, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000279 DAORPM010000000
VERSION     DAORPM010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11622_length_2963_cov_13.183975"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLD53_11100"
     CDS             complement(<1..317)
                     /locus_tag="PLD53_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRC61508.1"
                     /translation="MSKKLIIAEKPSVAADIARALGGFTKHDDYFESEDCVLSSAVGH
                     LLELACPEEYEVKRGKWSFAHLPVIPPHFDLRPIEKTVSRLKLLTRLIKRKDVTGLVN
                     ACDA"
     gene            complement(654..1745)
                     /gene="dprA"
                     /locus_tag="PLD53_11105"
     CDS             complement(654..1745)
                     /gene="dprA"
                     /locus_tag="PLD53_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRC61509.1"
                     /translation="MTECESLSSWLRLTLIPGIGGETQRKLLTAFGLPDAVFSADRSS
                     LRAVIGERSTRLLLDTDNAFLVESAKEWSQEADQRIVTLADPEYPQSLLEIPDPPTLL
                     YVRGRLDLLNRPALAIVGSRNPTPQGIQNAERFASTFADAGLVIASGLALGIDAAAHR
                     GALASTGDTIAFIGTGIDRIYPARNKELALEVASRGAVVSEFPIGTPVTAANFPRRNR
                     LISGIARGVLVVEAAIVSGSLITARLGGEQGREVFAIPGSIHSPQSRGCHKLIKQGAK
                     LVETAQDVLEELNWQTTLVAASPTNDKADGVPHALLSLMGFDPCSLDELAERSDLSAD
                     SLSVILLDLELSGRVASLPGGRYQQLQEV"
     gene            complement(1749..2816)
                     /locus_tag="PLD53_11110"
     CDS             complement(1749..2816)
                     /locus_tag="PLD53_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRC61510.1"
                     /translation="MIRIISMLVLAVAATLCNAAEQPLQLAENAPQRHIVVPGDTLWG
                     ISGQFLKEPWRWPEIWRMNKEQIKNPDLIFPGDVIVLDYDSNGNPMLRKQSGNSKLRQ
                     QSDKLHPQIYSEQISQAIPPIPPNAIGPYLSEPLVVEVDGLDSAARIVATQQDRVFLG
                     SGDTAYVNNADPSQLKWQVYRNGKPLLDPAFPNQNPADPTHVLGYEAFYLGTATQVSP
                     GSPATFQILTAKQEIGRGDRLVSAAQPSLVAYVPHKPDFMVDGRIISVYGGVESAGRG
                     SIVSLNRGSKEGLEIGHVLSVERNRTIVERDDDGRKVNVEIPPVRLGLVFVFRVFDHI
                     SYALIVQAESTVEVNDFVRTP"
BASE COUNT          699 a          814 c          794 g          656 t
ORIGIN      
        1 gcatcacagg cgttcaccag cccggtcacg tccttgcgct tgatcagtcg tgtgagcaac
       61 ttgaggcgtg ataccgtctt ttcgattggc cgcaaatcga aatgtggcgg aatgaccggc
      121 aaatgagcaa acgaccactt gccgcgcttg acttcatact cttccggaca ggccagttcg
      181 agcagatggc cgacagcgga cgataggacg cagtcttcac tctcgaaata gtcgtcgtgt
      241 ttggtaaacc cacctagtgc acgtgcaatg tcggcggcaa cggaaggttt ctcggcaatg
      301 atcagcttct tgctcatgga gtttatgtgc cagggagggc cgttagtcag tatgactata
      361 gtatggtcag gcagggcatg ataagtgcac tctacggcgc ttggcaagca acgcttgctt
      421 gcgcggagca atgcagacga tgaaaaccaa ttgctgcggc agatgtggaa gggtacggtg
      481 cgtcggtgcc ggatcaacat cgccgataca attcatacga caattgtgtt cccgattggc
      541 ggaatcgatc gatccccaag catcggaccg cggctggttt ccaactccct ccaactccct
      601 ttggcaggcg cgcaaagctg ggcgacactg aggcaggaag caaaccgacg aattcagact
      661 tcttgaagtt gctggtagcg gccacctggc agactggcca ctctgccact gagttccaaa
      721 tccaagagta tcactgacag cgaatcggcc gacaaatcac tacgttcggc aagttcatcc
      781 agactacacg gatcaaatcc catcaatgac aacagcgcat gaggtacacc atcagccttg
      841 tcgttcgtcg gcgatgcagc taccaaagtt gtttgccaat tcagttcctc aaggacgtcc
      901 tgcgcagttt caaccagctt cgcaccttgc ttgatcagct tgtggcagcc tctcgattgc
      961 ggtgagtgaa tcgatcctgg aatggcaaaa acctctcgtc cctgctcgcc gcctagtcgg
     1021 gcagtaatca gcgatccgct tacaatcgct gcttcaacga ccaacacgcc gcgggcgata
     1081 cctgatatca gccggttacg tcgcggaaag ttggctgccg tgaccggcgt gccgattgga
     1141 aactcggaaa cgacggcccc ccgactggcg acttccaaag ccagctcctt gtttcgcgcc
     1201 gggtagattc gatcaatgcc agtaccgatg aacgcgatag tatctccggt gctggccaag
     1261 gctccacgat gtgctgccgc atcgatacca agcgccagtc cgcttgcgat caccagaccc
     1321 gcgtcggcga aggttgaagc gaaacgttcg gcgttctgaa taccttgcgg ggtcggattt
     1381 cggctgccta cgatagccag agccggacgg ttaagcaggt ccaagcgacc gcgtacatag
     1441 agcagcgttg ggggatccgg aatttcaagc agcgactgcg ggtactctgg gtcagctaaa
     1501 gtaacgatgc gctgatctgc ttcttgtgac cattctttcg ctgattcaac gaggaatgca
     1561 ttgtctgtat cgagcagaag tctggttgac ctttcaccaa tgacggcgcg aagcgagctt
     1621 cgatcagcgg agaaaaccgc atcagggagc ccgaaagcgg taagcagctt gcgctgtgtt
     1681 tcaccgccaa tcccgggaat aagcgttagt cgcagccaac ttgaaagcga ctcgcattcg
     1741 gtcattactc agggcgtacg gacgaagtca ttgacctcaa cagtgctctc agcctgcacg
     1801 ataagtgcat aggatatatg gtcaaatacg cgaaatacaa aaaccaaccc gagccgaact
     1861 ggaggaattt ccacattgac cttgcgacca tcatcgtcac gttcaacaat cgtccgattc
     1921 cgctcaaccg acagcacgtg cccgatctct aggccctcct tggagccccg attcaatgaa
     1981 acgattgaac cccgcccagc agattcgacg cccccgtaga ccgaaatgat gcggccatcg
     2041 accatgaaat ccggcttgtg cgggacgtag gcgaccaatg aaggctgggc ggctgaaacc
     2101 aagcggtcac ctcgaccgat ttcctgctta gccgtcaaaa tttggaaagt ggccggactg
     2161 ccaggactga cctgtgttgc cgtacccaaa taaaatgcct catagccaag aacatgtgtc
     2221 gggtcagccg gattctgatt tgggaacgcc gggtcgagca acggctttcc gttacgatag
     2281 acctgccact tcaactggga agggtcggca ttgttaacat aagcggtatc gccactgcca
     2341 agaaataccc ggtcctgctg cgtagcgact atacgagccg cactgtccag accgtccact
     2401 tcaaccacaa gcggctcgga gaggtagggt ccgatcgcat tgggtggaat tggcggaatg
     2461 gcctgactaa tctgttcgga ataaatctga gggtgcagct tgtcactttg ctgacgcagt
     2521 ttggagttgc cactttgctt gcgaagcatt ggattaccgt tggaatcata atcaagaacg
     2581 atcacatcac cgggaaatat gagatccgga ttcttgatct gctccttgtt catgcgccag
     2641 atttccggcc agcgccaagg ctctttgaga aactgcccgg atatccccca taacgtgtca
     2701 ccaggcacga cgatgtgtcg ttgcggggcg ttttcagcca gctgaagcgg ttgttcagcg
     2761 gcgttgcata gtgtggcggc aacggccaga acgagcatgg atataatgcg gatcatcgtg
     2821 ttatcctcat acgagcggtc ggtaacatct gtttctgctt gtagacccac tgcgcaaata
     2881 ttgcgccgtt tttcaagatg ttgcatcgga ttctgcacgt aattacttct tcgatcaagc
     2941 tttttatgcc actactgcca ata
//
LOCUS       DAORPM010000280         2924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_11890_length_2924_cov_11.404322, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000280 DAORPM010000000
VERSION     DAORPM010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11890_length_2924_cov_11.404322"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PLD53_11115"
     CDS             <1..1194
                     /locus_tag="PLD53_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HRC61511.1"
                     /translation="HPATLLSILIYGYCTGVFSSRKLERATYDSVAFRYLAAGTHPDH
                     DTLATFRRRFFGEFSELFVQVLELAREMKLLKLGNVCLDGTKIQANASRHSALSHGHI
                     EKLETQLQAEVQELLALAEKTDQADVPDGIDLPAEIRRCEDRLAAMADAKTKIAARAE
                     ERYQREKPEYDAKLTKRTEKEKTTGKKPGGKPPAAPRPGPKDSDQINLTDEESRMMPV
                     SGGGFEQTYNAQAAADTETMLVVATGPTQAPNDKEQVKPMLETLAAQTQRLGKVTGLI
                     ADTGFCSETNISACETAGITPLIAVAKENHHPNWRERHSEPPALPENTTPVQTMAHRL
                     KTKAGRALYALRKQTVEPVFGIIKSVMGFRQFSLRGLTKVSGEWTLVCLAWNLKRLAV
                     LRLQE"
     gene            1504..1665
                     /locus_tag="PLD53_11120"
     CDS             1504..1665
                     /locus_tag="PLD53_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRC61512.1"
                     /translation="MTFAATSGNTVVDFGTAQWTVAEGSIPEPASVALLGIGLAGIGL
                     TRRRRKSAA"
     gene            1978..2226
                     /locus_tag="PLD53_11125"
     CDS             1978..2226
                     /locus_tag="PLD53_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRC61513.1"
                     /translation="MTYQILSEIAASISDLKKNPMGTVAAGEGFTVAILNHNVPAFYC
                     VPADVYESMMERLDDIELNAIADARAGQRVTKVSLDEL"
     gene            2216..2497
                     /locus_tag="PLD53_11130"
     CDS             2216..2497
                     /locus_tag="PLD53_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRC61514.1"
                     /translation="MSYELGFLDDALSEWRKLDGTVRAQFKKKLAERLEAPRVPTSRL
                     SGHQDRYKIKLRSIGYRLVYEVRDSQLIVVVVAVGKREKSAVYKAAEKR"
     gene            complement(2500..2634)
                     /locus_tag="PLD53_11135"
     CDS             complement(2500..2634)
                     /locus_tag="PLD53_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61515.1"
                     /translation="MDEQRFALSAGMQTNAEILLGTRSVMEYLLSPVRKAWHEAGRER
                     "
BASE COUNT          654 a          871 c          820 g          579 t
ORIGIN      
        1 cacccggcga cgctgctttc tattctgatc tacggctact gcaccggcgt cttctcaagc
       61 cgcaagctgg aacgggcgac ctacgactcg gtcgccttcc gctaccttgc cgccggtacc
      121 cacccggatc acgacacgct ggcgaccttc cgtcgccggt ttttcggcga attctccgaa
      181 ctgtttgttc aggtgctcga actggcccgg gaaatgaaac tgctcaaact cggcaacgtc
      241 tgcctggacg gcaccaagat acaggccaac gcctcgcgcc acagcgccct ctcgcacggg
      301 catatcgaga aactggaaac gcagcttcag gccgaagtgc aggaactgct cgccttggcg
      361 gaaaagaccg accaagccga tgtgcccgat ggtatcgacc tgccggcgga aatccgccga
      421 tgtgaagacc gtcttgccgc catggccgac gccaagacca agatcgccgc ccgggccgaa
      481 gaacgttatc agcgggagaa gcccgagtac gacgcgaagc tgaccaaacg caccgagaaa
      541 gaaaagacca cgggcaagaa acccggcggc aaaccgcctg ccgccccgag acccggaccg
      601 aaagacagcg accagatcaa tctgaccgat gaagaatccc ggatgatgcc ggtttccggc
      661 ggtggcttcg aacaaaccta caacgcacaa gccgcggcag atactgaaac gatgctcgtt
      721 gtcgccaccg ggccgacaca agcgcccaac gacaaagaac aggtcaaacc gatgctggag
      781 accctggcgg ctcaaaccca gcgactgggc aaggtcaccg gcctgatcgc cgataccggc
      841 ttttgcagcg agacgaacat cagcgcatgc gaaacggccg gcatcacccc gctaatcgcg
      901 gtagcgaaag agaatcatca tccaaactgg cgcgaacgcc acagcgagcc gccggccttg
      961 ccggagaaca cgacacccgt ccagacgatg gcgcatcgcc tgaagacgaa ggccggacga
     1021 gcgctctatg cgctacgcaa gcaaacggtc gaaccggtct tcggaatcat caagtcggtc
     1081 atgggatttc gtcagttctc gctgcgcggc ctgacgaaag tcagtggcga atggacgctg
     1141 gtctgcctcg cgtggaattt gaaacgcttg gccgtattgc gtcttcagga gtgagaatgc
     1201 tttgaaaaca cccggaaaat cgaaaaatgc agccacgaaa ccgcgaaaac cgctccgcag
     1261 ttcacttatc tgtcgtcggg ccagcatcag tccgacaggc tgctagcttg gagcgttggg
     1321 accggtgcgt tcgccacgcc cactagcaac tacgataact ataattttcc tacaggcgga
     1381 tatccggctt ttgacaactt gccacttgcc gccggcgcct ctttcgactt cgatttcttc
     1441 caattcatcc cgatcgctgg cggcgccgca ttcggatcgt acactggcca aaatcctttc
     1501 agcatgacgt tcgctgccac tagtggcaat acggttgtcg attttggaac tgctcagtgg
     1561 accgttgcag aaggttcaat tcccgaaccc gcaagtgttg cacttctcgg cattgggctc
     1621 gcaggcatcg gcttgacgcg gcgccgccga aagtcagcag cgtaggagtg ccaccacttc
     1681 ccgaaccctt ggaaccccgc ttcggcgggg tttgtctttg tcagcgcagc tgatcgacct
     1741 tgggccgcct tacgccggtt catccagcca ggcatcacgc ggcaggtcgg cggttccggg
     1801 ggtgatcacg aagcgcgtga acaatacgga acaatagggg acacctatta gccggccccg
     1861 tgtcaagggc gaatttatcg atccaacaga tgaagccggc ttcaccattg taattcgcac
     1921 ttgcttcggt tttttagagt gctagtatta gcaccttaaa atggtcttgg agccaatatg
     1981 acttatcaaa ttctttcgga gatagcggcc agcatttccg acttgaagaa aaatccgatg
     2041 ggtactgtcg ccgccggcga ggggtttacg gttgccattc tgaatcacaa cgtacctgcc
     2101 ttctactgcg tgccggctga cgtgtatgag tcaatgatgg aacgactcga cgacatcgag
     2161 ctgaatgcca ttgctgatgc acgggccggc cagcgcgtga cgaaggtttc gctcgatgag
     2221 ctatgagctt ggctttcttg acgatgcgtt gagcgagtgg cgcaagctgg atggcacggt
     2281 ccgtgcccag ttcaagaaga aactcgccga acgcctggag gcaccgcgag ttccgacgtc
     2341 caggctgtcc gggcatcaag atcgctacaa aatcaagctg cgcagcattg gctatcggct
     2401 tgtgtacgaa gtccgcgaca gccagttgat cgttgttgtt gtagcggttg gcaagcgtga
     2461 gaaaagcgcc gtttacaaag ccgccgagaa gcgatagccc taccgctccc tccccgcctc
     2521 atgccacgcc ttgcgcacgg gcgacagcag atactccatg accgagcggg taccgagcag
     2581 gatctccgca ttggtctgca tccccgcact caaggcaaag cgctgctcat ccatcgagag
     2641 attcgaaggg gatgcggaac aatccgcaca ggctgcccag caaccacgcc atatccgcct
     2701 gcctgacttc cccgcgaggg ttcattttct tgtcgttttt gtggaacgcg cggagcatga
     2761 tcccacttca ttggaatgtc aagaaatgtg ctctttttat gtcattagcg ttgcaagttg
     2821 tcgtgatttg taagcaagct gttcggaacg cgtatccgat ttgtcagggc gtttgggcgg
     2881 gatggtggat gtgggcaagc tgtcctcctc ccgcgcggtt gtcg
//
LOCUS       DAORPM010000281         2881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_12153_length_2881_cov_11.123496, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000281 DAORPM010000000
VERSION     DAORPM010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12153_length_2881_cov_11.123496"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLD53_11140"
     CDS             complement(<1..873)
                     /locus_tag="PLD53_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRC61516.1"
                     /translation="MAEVSKSSVTQAIISEDEQGQRLDNFLLKICKGVPKSHIYRIVR
                     SGEIRINSKRAEVSYRLQNGDILRIPPIRIARRQEEIVAGAEIKVALPIVYEDEAMLV
                     VDKPSGIAVHGGSGVSFGVIEALRRQRPQAHFLELAHRLDRETSGILLVGKKRSALTA
                     LHDMFREGGLAGRGRGVDKRYLVLVKGRWPDQVRNVKLPLLKYLLESGERRVRVSDEG
                     KASHTVFRLIARWEQFSLLEAELKSGRTHQIRVHLAHLGFPIAGDEKYGDFTLNKSLP
                     NAGLKRMFLHAWKMQ"
     gene            1275..>2881
                     /locus_tag="PLD53_11145"
     CDS             1275..>2881
                     /locus_tag="PLD53_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRC61517.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLVDLDIESATKEQKKSNIYKA
                     VITRIEPSLEAAFVDYGSERHGFLPFKEISRSYFQAGIEASRATIKEALREGQELIVQ
                     VDKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGDERAELRDVMDQLQVP
                     QGMSLIARTAAIGRNAEELQWDLNYLMQLWKAIDGAANQQKGAFLIYQEGSLVIRAIR
                     DYFQPDIGEILIDTDDVFEQASQFMSHVMPGTVNRIKRYRDDVPLFSRFQIEHQIESA
                     YSRQVILPSGGAIVIDHTEALVSVDVNSGRSTRGADIEETAYKTNLEAAEEVARQLRL
                     RDLGGLIIIDFIDMESQRNQREVENRLRDGLRLDRARVQTGKISRFGLMELSRQRLRP
                     ALAETSYIPCPRCSGTGHIRSAESAALHILRILEEEAMKDNTATVHSQVPVDVATFLL
                     NEKRTDIQAIELRHKVSILLIPNVHLETPQHSIIRLRHDELNQDEIQQPSYKMVDVPS
                     EEATRQASIAGEVGSPRQEAAIKGITT"
BASE COUNT          674 a          808 c          758 g          641 t
ORIGIN      
        1 ctgcattttc caggcgtgca gaaacatgcg tttaagcccc gcattcggta gtgacttgtt
       61 cagtgtgaaa tccccatatt tttcatcacc ggcaatcggg aaaccgagat gcgcaagatg
      121 gacgcgtatc tggtgcgtgc gtccggattt gagctcggct tccagcaagc tgaattgttc
      181 ccagcgggcg atcagtcgaa agacggtatg cgatgctttg ccttcatcgg aaaccctgac
      241 acgccgttca cccgactcca gcaaatactt gagtagtggc agcttcacat tacgtacctg
      301 atcaggccag cgtcccttga ccaggaccag atagcgcttg tcgacgccac gcccacgacc
      361 tgctagccca ccttcacgaa acatgtcgtg cagtgcggtc aatgccgaac gtttcttgcc
      421 aaccagcagg attcccgagg tttcgcggtc aagccgatgc gccagttcaa ggaagtgcgc
      481 ttgcggtcgc tgtcgccgta gtgcctcaat gaccccaaaa ctgaccccgc tacccccatg
      541 cacggcaatg cctgagggct tgtccacgac cagcatggct tcgtcttcat agacgatcgg
      601 caatgccacc ttgatctctg ccccggcgac gatttcttcc tgtcgacggg caatacgaat
      661 cgggggtatc cgcaggatat ccccattctg taagcgatag gagacctctg cccgcttgct
      721 gtttatccgg atttcaccgc tacgaacaat acggtagata tggcttttag gcacaccctt
      781 gcaaatcttc aacaggaaat tatcgaggcg ttgcccctgt tcgtcttcac tgatgatggc
      841 ctgtgtaaca gaacttttgc ttacttcggc catattgaat atactgatcc tggtttgcgt
      901 cttcgtttga aagaggcgct gagccgttag accgtcgagc ggcgccgatt cttttgtaat
      961 tgcgacgcac tttccggcag gcatcagccg ccttgcttgc accaacctgt gcaagatcaa
     1021 atagcgcgaa tcaactggtt aagtacgctc accacaagta gcgatactac tagatttgta
     1081 cgcgcctagc acgttcggat cgcctcaagc agattcgtga gccttgattt ttcagggttt
     1141 gaacaagaat aaattcagcg agacatgctt attagttgcc ataccggaaa gcccatgaag
     1201 cgtcccaccc acagctaatc catcgctgcc tgcacaaggc gctccccccg tgattgcgcg
     1261 tgggagccca ttttatgaaa cgcatgctat ttaatgcgac acaggccgaa gaactccgtg
     1321 tcgccattgt tgatggtcag aaactcgttg atctggacat tgaatcggcc accaaggaac
     1381 aaaagaagag caatatctac aaggccgtca tcacacgtat cgagcccagc ctcgaagccg
     1441 cgtttgttga ttatggttct gaaaggcatg gctttcttcc gttcaaggaa atctcccgct
     1501 cgtattttca ggcaggcata gaggccagcc gggcaaccat caaggaagcc ctgcgtgagg
     1561 gccaagagct gatcgttcag gttgacaagg acgagcgcgg aaacaaaggg gctgcgctga
     1621 caacttttat cagtctggcc ggacgttacc ttgtgctgat gcccaacaac ccaagaggcg
     1681 gtggcgtttc gcgccgggtc gaaggtgatg aacgcgccga actgcgcgac gtaatggatc
     1741 agttgcaggt gccacagggc atgagcctga ttgcccgcac ggcggccatc ggtcgcaatg
     1801 ccgaagaact gcaatgggac ctcaattacc tgatgcagtt gtggaaggca atcgacggtg
     1861 cggccaacca acagaaaggg gcgttcctca tttatcagga aggaagcctg gtcattcgtg
     1921 ccattcgtga ctacttccag cccgacattg gtgaaattct gattgatacc gacgacgttt
     1981 tcgaacaggc gagccagttc atgtcacacg tcatgccggg cacggtcaat cgcatcaaac
     2041 gctaccgcga tgatgtaccg cttttttcgc gcttccagat cgagcaccag atcgagtcgg
     2101 cttactcccg tcaggttatc ctgccctcgg gtggtgccat tgttatcgat cacaccgagg
     2161 ctctggtttc ggttgacgtg aactcaggcc gctcgacgcg tggtgcagat attgaggaaa
     2221 cggcctacaa gaccaacctc gaggccgcag aagaagtcgc tcgtcagtta cggctccgcg
     2281 atctgggggg cctgatcatc atcgacttca tcgacatgga gagtcagcgc aatcagcgtg
     2341 aagttgaaaa ccgcctgcgc gatggcctgc gtcttgatcg tgcgcgggtg cagaccggaa
     2401 aaatcagccg cttcggattg atggaactgt cgcgtcaacg attgcgcccg gcgcttgctg
     2461 aaaccagtta tatcccctgt ccacgctgtt ccggcaccgg ccatattcgc agcgcagaat
     2521 cggccgcgct gcacattttg cgaatccttg aagaagaggc gatgaaggac aacaccgcta
     2581 ccgtacattc acaggtgccc gttgatgtcg ctacctttct gctcaacgag aaacgcacgg
     2641 atatccaggc catcgaattg cggcacaagg tcagcatact gctgatcccc aatgttcatc
     2701 tggaaacgcc gcagcacagc attattcgtc tgcgtcatga cgaattgaat caggacgaaa
     2761 tacagcaacc ttcatacaag atggttgatg tgccctccga agaagccacc aggcaagcgt
     2821 ctatcgcggg tgaagtcggg tcgccacgtc aggaagcagc gatcaagggc atcacgacaa
     2881 g
//
LOCUS       DAORPM010000282         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_12411_length_2842_cov_9.030140, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000282 DAORPM010000000
VERSION     DAORPM010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12411_length_2842_cov_9.030140"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLD53_11150"
     CDS             complement(<1..329)
                     /locus_tag="PLD53_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61518.1"
                     /translation="MNPGAELQYELGRAVGTLRRRSASTSDTTEKKALSRIIQRLDKQ
                     LAALDRADLLTVAKALADGIDALDDAVAAVKRGPFDGYLSALEGHFDDLSSLSAALHG
                     LDALARTP"
     gene            complement(353..2305)
                     /locus_tag="PLD53_11155"
     CDS             complement(353..2305)
                     /locus_tag="PLD53_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HRC61519.1"
                     /translation="MALHVFVAMPYGRSQNIDFDAVYADYLKPALETAGFEVFRADEE
                     ERAGDIRTDMFQELLLADLVVVDLTLDNPNVWYELGVRHALRAHGVLLVQSERAYQPF
                     DIYTDRKLHYHLKQGRPDPDHLEADRKSLTNMALATMQSRNERPISPVFRLLDGLSEP
                     AWRKLLVTGNNEFRQVYESWHRRIEIARKGNRPGDIMVLAEETPTWALRLEARRIAGT
                     ALMKLNQYQLALDQFDAAIELDPADEHNQREKSILLGRLGRHDEAREVVNALIRQHGN
                     SPENWRLLGRLETEDWVQRWRLPDSSPESRREKAGRELPQLIQAIEPCMNAFISDPAH
                     FISGINACTLRHVQIHLGYALANPASLPNLEGGVIWSCLAALERQPGDYATRAAWAEL
                     TILFNPPGAVEKAWREAVAVANKDWHALETSRQQLLILRDLEFRTQNVETALAVLDEE
                     IASLEAPWQARRVFLFSGHMIDTPDRAEPRFPASQEKTAAAAIAAKLDELGMGEGGER
                     DLAICGGACGGDTLFAEAALRRGCRVHLHLQFIEADFLQASVAFAGESWIDRYYAIKE
                     HALARIRLQLDELGPPPKGSNPYVRNNLWQLYTALAHGVDKVRFVGLWNGEGGSGPGG
                     TQNMVETVRRYAGRVSIIDTRQLFGL"
     gene            complement(2430..>2842)
                     /locus_tag="PLD53_11160"
     CDS             complement(2430..>2842)
                     /locus_tag="PLD53_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61520.1"
                     /translation="TGNGQRQDDLHEFWPNQEVDMDDKSEVYDVPAIRAKLLEKMKDD
                     PGAYPTLIEQRFPRILAKIADLWGKPALDQYLDVLMFTDRSDRQGFPPEVAVEVFRLS
                     TAHAALNLVRQAKVTGWAGVDEAVLDKKAFAKDE"
BASE COUNT          509 a          863 c          867 g          603 t
ORIGIN      
        1 ggggtgcgcg ccagcgcatc caggccgtgc agggcggcgg acaggctgga gaggtcgtca
       61 aaatgacctt caagcgcgct caggtagccg tcgaacggcc cgcgcttgac tgccgctacg
      121 gcgtcgtcca gtgcgtcgat gccatccgcc agtgccttgg caacggtgag caggtcggcc
      181 cggtccagcg ccgcaagctg cttgtcgagg cgctgaatta tcctgctcag ggccttcttt
      241 tcagtcgtat cgctggtact ggcgcttcgc cgccgcagcg taccgactgc gcgaccaagt
      301 tcatactgaa gttccgcgcc tggattcatg gcgtatctcc ctgtctgaat cgtcaaagtc
      361 cgaacaactg ccgggtatcg atgatgctga cccgtccggc ataccgcctg actgtttcaa
      421 ccatgttctg cgtgccgccg ggcccggaac cgccctcgcc attccacagg ccgacgaagc
      481 gcaccttgtc gacgccgtgt gccagcgccg tgtaaagctg ccaaaggtta ttgcgcacat
      541 aggggttgct gcccttgggg ggagggccca actcgtccag ttgcaggcgt atccgggcaa
      601 gggcatgttc cttgattgca taatagcgat cgatccagga ttcgccagcg aacgcaacgg
      661 aagcctgcaa aaagtccgct tcaatgaact gcaggtgcag gtgaacccgg caaccgcggc
      721 gaagtgccgc ttcggcaaac agggtgtcgc ccccacacgc gccgccgcag atggcgagat
      781 cccgctcgcc tccttcaccc attccgagtt catcgagttt ggcggcgatg gcagccgccg
      841 ccgtcttctc ctgcgacgcg ggaaaacgag gttcggcccg atcgggcgta tcgatcatgt
      901 gtccgctaaa caggaaaacc cggcgcgcct gccaaggtgc ttcgaggctg gcgatttcct
      961 catccagcac cgccagtgcg gtttccacgt tttgtgtgcg gaactccagg tcgcgcagga
     1021 tgagcagttg ctgccggctc gtttccagtg catgccagtc cttgttggcg acagctaccg
     1081 cctcgcgcca ggctttctcc accgcgccgg gtggattgaa caggattgtc agttcggccc
     1141 aggcagcgcg agtcgcatag tcgcccggct ggcgttccag agcggccagg caggaccaga
     1201 ttacgccgcc ttcaagattg ggcaggctgg ccggattggc cagcgcgtat ccgaggtgga
     1261 tctgcacatg gcgaagagtg caggcgttga tacccgaaat gaaatgcgct ggatcactga
     1321 tgaaggcgtt catgcagggc tcgatggcct gaatgagctg cggcagctcg cggcctgcct
     1381 tttcgcgcct gctttccggg ctgctgtcgg gcagacgcca gcgttgtacc cagtcttccg
     1441 tttcgaggcg gccaagcagt cgccagtttt ccgggctatt gccgtgttgg cggatgaggg
     1501 cgttgactac ttcgcgggct tcgtcatggc gtccgaggcg gccgagaaga atggatttct
     1561 cgcgctggtt atgctcgtcg gcggggtcga gctcgatagc cgcatcgaac tgatccagtg
     1621 ccagctgata ttgattgagc ttcatcaacg ccgtgccggc tattcgccgt gcttcaaggc
     1681 gcagagccca ggtcggcgtt tcttcagcca gcaccatgat gtcacctgga cggttgcctt
     1741 tgcgcgcgat ctcgatgcgc cggtgccagg actcgtagac ttggcgaaat tcattgttgc
     1801 cggtcactag cagtttgcgc caggccggct cgctgagccc gtccagcaac cggaagaccg
     1861 ggctgatcgg tcgttcattc cgggactgca tggtggccag tgccatgttg gtcagcgact
     1921 tgcgatcagc ctccagatga tccgggtcgg gtcgtccctg cttgaggtga tagtgcagtt
     1981 tgcggtcggt gtagatgtcg aaaggctgat acgcgcgctc gctttgcacc agcagtacgc
     2041 cgtgtgcccg cagcgcatgg cgtacgccga gctcgtacca gacattgggg ttgtccagcg
     2101 tcagatcgac aaccaccagg tcggccagca gcaactcctg gaacatgtcg gtccggatgt
     2161 cgccagcgcg ctcctcttcg tcggcgcgaa acacctcgaa gcctgcagtt tccagcgccg
     2221 gtttgagata gtcggcgtag accgcatcaa aatcgatgtt ctgactgcgg ccgtaaggca
     2281 tggccacgaa aacgtgtaac gccatatggc ctctcctgaa tgcttgtcaa tccttgccga
     2341 accgcgtccg gattgccgca ccccttgtca aggaacccgc caggcaaatg cgacaaagcc
     2401 tgtatctgcg caggcttttg ttgtgcttcc tattcgtcct tggcgaaagc ctttttatcg
     2461 aggaccgctt cgtcgactcc agcccagcct gtgactttgg cttgcctgac gagattgagg
     2521 gcggcgtgag cggtggacag gcggaatacc tccaccgcca cctccggcgg aaacccctgc
     2581 cggtcactgc gatccgtaaa catcaggaca tcgagatatt gatcaagggc aggtttgccc
     2641 cagagatcgg ctattttggc gagaatgcgc gggaaacgct gctcaatcag cgttgggtaa
     2701 gcaccagggt cgtctttcat tttctccagc agcttcgccc tgatggccgg aacatcgtag
     2761 acttccgatt tgtcgtccat gtccacctcc tgatttggcc agaattcatg cagatcgtct
     2821 tggcgctgac cgttgccagt gc
//
LOCUS       DAORPM010000283         2829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_12494_length_2829_cov_8.952415, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000283 DAORPM010000000
VERSION     DAORPM010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12494_length_2829_cov_8.952415"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..644
                     /locus_tag="PLD53_11165"
     CDS             312..644
                     /locus_tag="PLD53_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61521.1"
                     /translation="MMTKRALLALSGLVLVLAGCASDQQLLANDQTNAVQAAVRRGQF
                     EMSCPGATGTMLSNTLLQPVLWNGIERAEYTVGVQGCGQKSTYVVVCQVGSASCLAVS
                     GRNQAFTQ"
     gene            complement(736..>2829)
                     /locus_tag="PLD53_11170"
     CDS             complement(736..>2829)
                     /locus_tag="PLD53_11170"
                     /inference="COORDINATES: protein motif:HMM:NF018620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HRC61522.1"
                     /translation="LVYDATRLSEAPPTRLFIDAQLASQWRQKGFYPVLNERTPSPEN
                     NLAASVLYRSLALKKTHPLPDDEKVLSSAFDFSLGRSQSCPRLDQYDAYERDHPLGGM
                     PYGLPGLSQTELDTVTRWLAAGSPEDAPPALPPAVVQQVRDWERFLNGDSLKERLMSR
                     YLYEHLFLGHLIFENDPQRRSFRIIRSADAPHASDAPARPIATRRPYDDPGVARVYYR
                     LVLDHETIVAKTHMPYLLSPTRMNKYRGWFLNPAYPVDTLPSYAIEQASNPFITFAAI
                     PPDSRYRFLLDEAEFFVMNFIKGPVCRGQMALDVIESRFWVFFVNPDSDFSAENAEIL
                     ARQASNLRLPAAYGSDAPILTAWHELSRLEKKVLAAKSAILNQRFSGERKIDLSLIWD
                     GDGQNPNAALTIFRHFNSASVVKGLVGDAPKTSWVIGYPLFERIYYLLVAGYDPYGNI
                     GHQLDSRLYMDFMRMEGESNFLTLLPKGVRKSTRDYWYRGASGDEKSYIDGSKNPLDE
                     ETGIAYRSKDPQHELYGFLGSRLASVLGSRFKLASVPNAALRKELQHLTSLRGASLSW
                     LPPATVLRVDDPPHAPQYFSLLKNVGHANVSHLLLERSELLPDENTLTVVPGFIGAYP
                     NAIYHATPAELPALASAIKKLASEADYRALADRFVIRRTNPEFWTASDALIEAYARWA
                     PLEAGLFDYSRLENR"
BASE COUNT          584 a          796 c          874 g          575 t
ORIGIN      
        1 gaagcccaag cactctccgg agaattgagc agtgccaaga atctgctggt gccgctcgat
       61 cagtatttga agaagaccga gagtggtgcg aaaaagaaat agcgacctta tcgcttacgg
      121 gaaggcgtaa gtattgggtc cggcgtactt gcattcgggt tctgcccaca tctggtgcgg
      181 cgacacgtta tgtcacaact gatcgagacc ctcggtaggg ccgtgtccgg tctaattaga
      241 aacccagatc gagcggctac cggaaaagcg tttaagttcc tatactgggt tttttacaag
      301 cggagagaat catgatgacg aaaagggcgt tacttgcatt atccggtttg gtgctcgtac
      361 tggccggctg cgccagtgac caacaactgc ttgccaacga ccagaccaac gcggtacagg
      421 ccgccgtacg tcgtggtcaa ttcgagatgt cctgccccgg cgcgaccgga acgatgcttt
      481 ccaacaccct gcttcagccc gtgctctgga atggcatcga acgtgccgaa tatacggttg
      541 gcgttcaggg atgcggccag aaatccactt acgtcgttgt ttgccaggtg ggatcagcca
      601 gttgccttgc ggtgtccgga cgaaaccagg ccttcacaca gtaagctatc tcctgggtcg
      661 tgtggtggat agaaatctca cctaagccgc cacgctggca gctgccggaa acttccgaaa
      721 atgtaaccct gggccttagc ggttttcaag ccggctgtag tcgaacaatc cggcttcgag
      781 tggtgcccat cgtgcgtagg cttcgatcag cgcatcgctc gctgtccaga attccggatt
      841 ggtgcgccgg atcacgaagc ggtcggccag tgcccggtaa tcggcctcgg atgcgagctt
      901 tttgatggcg ctggcaagtg ctggcaattc cgctggcgtc gcgtggtaga tggcgttcgg
      961 atacgccccg ataaagccgg gtacgacggt cagcgtgttc tcatcgggca gcaattcgga
     1021 tcgctccaac aacaggtgcg acacattcgc gtggccgaca tttttcagca gactgaaata
     1081 ttgcggtgcg tgcggtggat catcgacccg caacaccgtg gccgggggca gccacgacag
     1141 gcttgcaccc cgcagcgatg tcaggtgctg caactccttg cgcaacgccg cattcggcac
     1201 gctggccagc ttgaaacgcg agccgagaac cgatgccagc cggctaccca gaaaaccgta
     1261 gagttcgtgc tgcggatcct tgctgcggta ggcgatgccc gtttcctcgt ccagcggatt
     1321 cttgctgccg tcaatgtagg acttttcgtc gccgctcgcc ccccgatacc agtagtcgcg
     1381 cgtcgatttg cggacgcctt tgggcaacag cgtcagaaag ttgctctcgc cttccatgcg
     1441 catgaagtcc atgtacagac ggctgtcgag ctggtgcccg atgttgccgt aggggtcata
     1501 gccggcgacc agcaggtagt agatccgttc gaacagcggg tagccgatca cccaggatgt
     1561 cttcggtgca tcgccgacca gtcccttgac caccgatgcg ctgttgaagt gcctgaaaat
     1621 cgtcagcgcg gcattcggat tttgcccatc accgtcccag atcagcgaca ggtcgatttt
     1681 gcgctcgccg ctgaaacgct gattgagaat cgcgctcttg gcggccagca ccttcttttc
     1741 gagcctggag agttcgtgcc atgcggtgag aatgggcgca tcgctaccgt aggcggcggg
     1801 caagcgcaga ttgctggctt ggcgggccaa tatctccgcg ttctccgcac tgaaatcgga
     1861 gtccggattg acgaagaaaa cccagaagcg ggactcgatt acgtcgagcg ccatctggcc
     1921 gcgacaaacc gggcccttga tgaagttcat gacgaagaac tcggcctcat cgagcaagaa
     1981 gcggtagcgt gaatcaggcg ggatcgcggc aaaagtgatg aaggggttcg aggcctgttc
     2041 gattgcgtag gacggcagcg tatcgacggg atacgccgga ttgaggaacc agccacgata
     2101 cttgttcatg cgtgtcggcg ataacagata cggcatgtgc gtcttggcga cgatggtttc
     2161 gtgatcgagt acgaggcggt aataaacccg tgcaacgccc gggtcatcgt agggacgacg
     2221 tgtcgcgata ggccgcgccg gcgcatctga cgcatgcggc gcatcggccg atcggatgat
     2281 gcggaacgag cgccgctgtg ggtcattctc gaagatcagg tgaccgagaa acaagtgctc
     2341 atacaggtag cgactcatca gccgttcctt gagcgagtcg ccgttgagaa accgctccca
     2401 gtcgcgcacc tgttgtacga cagcgggcgg caatgccggc ggcgcatcct ccggcgagcc
     2461 agcggccagc cagcgtgtca ccgtgtcaag ctcggtctga cttagccccg gcaatccgta
     2521 gggcatcccg cccaagggat gatcgcgttc ataggcgtcg tactggtcca gacgcgggca
     2581 cgactgcgag cggccgagcg aaaagtcgaa ggccgacgac aacacctttt catcgtcggg
     2641 aagcgggtgc gtcttcttga gtgcgagact gcggtacaga acgctcgccg ccagattgtt
     2701 ttcgggagac ggtgtgcgtt cgttcaatac cggatagaaa cccttctgtc gccattgcga
     2761 tgccagctgg gcgtctatga agagacgcgt cggcggagcc tcgctgagac gcgtggcgtc
     2821 gtagacaag
//
LOCUS       DAORPM010000284         2813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_12614_length_2813_cov_10.968455, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000284 DAORPM010000000
VERSION     DAORPM010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12614_length_2813_cov_10.968455"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLD53_11175"
     CDS             complement(<1..217)
                     /locus_tag="PLD53_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRC61523.1"
                     /translation="MKLVILDRDGVINYDSDQFIKSPSEWRPIPGSLEAIARLSHDGY
                     RVVVASNQSGIGRGLFDMDTLNAIHDKM"
     gene            complement(214..2313)
                     /gene="glyS"
                     /locus_tag="PLD53_11180"
     CDS             complement(214..2313)
                     /gene="glyS"
                     /locus_tag="PLD53_11180"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRC61524.1"
                     /translation="MSNSVLIELRTEELPPKALSHLGEVFKDEIFNGLVRCQLSQRVP
                     ANVSWFATPRRLAVSVREVETTAADRVEEMKLMPVSVALDANGQPTQALRKKLEASGI
                     PVSEIAKFETRMDGKAQALFYKATVKGVDLDAVLAGIVTQALKKLPIQKVMRWGDSED
                     QFVRPVHSLIMLHGTRVVPGTVLGLTSGNKTLGHRFLSSGDIVIENPEEYAAKLEAGK
                     VIASFAERRASIAAQLDVKAAELNARLNPAEGLLDEVTSLVEWPVVYVGEFEADYLEV
                     PQECLILTMQQNQKYFPLLDSAGKLLNKFLIVSNMQVDDPTNIISGNARVVRPRLSDA
                     RFFYNQDRKHSLASRLDRLNSVVYHNKLGSVLQRVDRLEGLARKIGISLGANASLAAR
                     AARLAKADLVTDMVGEFPELQGTMGRYYALHDGEDARVADAVESHYQPRFAGDVLPQG
                     SIACATALADKLDALAGFFGIGQIPTGDKDPFGLRRAALGILRILMESPLPLDLRELL
                     ADAASGFEPGQLLLTDGLPPGLEDFMLDRLRGLLRDAGYAQDVIEAVLVQRPTRIDLV
                     PARLDAVRSFQALPEALALAAANKRIGNILKKAESAGGERFSKPDSALLQEDAEKALY
                     QRVQEISPVVESHVDNEAYADALQALASVRVEVDHFFDKVMVNVDDSSVRANRLGLLK
                     ALFDQLNAVADISKLSV"
     gene            complement(2310..>2813)
                     /locus_tag="PLD53_11185"
     CDS             complement(2310..>2813)
                     /locus_tag="PLD53_11185"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRC61525.1"
                     /translation="MEVTQFTYFQEVGSLACKPVLGEITYGIERLAMYLQGVENVFDL
                     TWAEGPGYRVTYGDVYHQNEVEQSKYNFEHSNIEMLFRHFSDHESEAKRLMGLDLALP
                     AFEQVMKCSHCFNLLDARGAISVTERAAYIGRVRALAREVAQSYYNSREALGFPMCKA
                     TAAVEAI"
BASE COUNT          569 a          814 c          847 g          583 t
ORIGIN      
        1 gcatcttgtc atgaatggca ttgagcgtgt ccatgtcgaa caggccgcga ccgatgcctg
       61 actggttgga ggcgaccacg acacggtagc cgtcatgcga cagtctggcg atggcctcca
      121 ggctgcccgg aatgggtctc cactccgacg gcgactttat gaattggtcc gagtcgtaat
      181 tgatgacgcc gtcgcgatcg aggataacga gtttcatacg ctcagtttcg aaatgtcggc
      241 aactgcattg agttggtcga ataatgcctt gagtagacca agacgattgg cccgtacact
      301 ggaatcatca acgttgacca tcaccttgtc aaagaaatga tcgacctcga cacgaaccga
      361 ggccagcgcc tggagggcgt cggcatacgc ttcattgtcg acgtgtgatt cgaccaccgg
      421 ggatatctcc tgaacccgtt ggtaaagtgc cttctcggca tcttcctgca gcagggctga
      481 atcgggtttg gaaaatcttt caccaccggc agattcggct ttcttcagga tgtttccgat
      541 acgcttgttg gccgcagcca gcgccaacgc ctcgggcagt gcctggaagg agcgcacggc
      601 gtcgagtcgg gccggaacga gatcgatccg cgtcgggcgc tgcaccagta cggcttcgat
      661 tacatcctgc gcgtaacccg cgtccctgag cagaccgcga agacgatcaa gcatgaagtc
      721 ctcaagaccc ggaggaaggc cgtcggtcag caacagttgg ccgggctcga agccggaagc
      781 agcatcggcg agaagctccc gtaaatcgag gggcaatggt gattccatca ggatgcgcag
      841 gataccaagc gcggcacggc gcagaccgaa ggggtccttg tcgccggtcg ggatctggcc
      901 aattccgaag aaacccgcca gggcatcgag cttgtcagcc agcgcggtgg cgcaggcaat
      961 gctgccttgc ggcaacacat cgccggcaaa gcgcggctga tagtggctct cgacggcgtc
     1021 ggcaacgcgc gcgtcctcgc catcgtgaag cgcgtaatag cggcccatgg tgccctgcaa
     1081 ctctgggaac tcgccaacca tgtcggtgac taggtcggcc ttggccagtc gcgccgcgcg
     1141 cgcggcaagg gaggcgtttg cgccgaggct tatgccaatc ttccgggcca agccttcgag
     1201 gcgatcgacg cgttgcagca ccgacccgag cttgttgtgg tagacgacgc tgttcagacg
     1261 gtcaaggcgc gaagccaacg aatgcttgcg gtcctgattg taaaagaagc gggcatccga
     1321 caggcgggga cgaaccacac gcgcattgcc gctgatgatg ttcgtcggat catcgacctg
     1381 catgttggag acgatcagga atttgttcag cagcttgccc gcgctatcga gcagcggaaa
     1441 gtacttctgg ttttgctgca tggtcagaat caggcattcc tgcggaacct caaggtagtc
     1501 ggcctcgaat tctcctacgt agaccaccgg ccattcgacc agcgaggtga cttcatcgag
     1561 caggccttca gccggattca ggcgcgcgtt cagttcggcg gctttgacgt cgagctgcgc
     1621 ggcaatcgac gcgcgccgtt cggcgaacga ggcgatgacc tttccggctt caagcttcgc
     1681 tgcgtactct tcggggttct caatgacgat atcgccggaa gaaaggaagc ggtgtcccag
     1741 tgtcttgttg ccactcgtga gtccaagcac cgtgcccgga acgacgcgcg ttccgtgcag
     1801 catgatcagg ctgtgaaccg ggcgtacgaa ctggtcttcc gaatcgcccc agcgcatgac
     1861 cttctggatc ggcagctttt tgagcgcctg cgtgacgata ccagcgagca ctgcgtcgag
     1921 atcaacaccc ttgaccgttg ccttatagaa cagcgcctga gctttgccgt ccatgcgcgt
     1981 ctcaaatttg gcgatttcgg aaaccgggat gcctgatgct tcaagcttct tgcgcagcgc
     2041 ttgtgtcggt tgaccgtttg cgtcgagtgc gacggagaca ggcatgagtt tcatttcttc
     2101 gacgcggtca gctgcggtcg tctcgacttc acgaaccgag accgccagtc tacgcggtgt
     2161 agcgaaccac gatacgtttg ccggcaccct ctggctgagc tggcagcgca ccaatccgtt
     2221 aaatatttcg tccttaaaaa cttcgccgag atgtgacaag gccttcggcg gcagttcttc
     2281 ggtacgcagc tcgatcagca cactgttgct catatcgcct ccacagcagc agttgccttg
     2341 cacatcggga accccagggc ttcgcgcgag ttgtagtagg actgcgcgac ttcgcgtgcc
     2401 aaggcgcgaa cgcggccaat gtaggcggcg cgttcggtga ccgaaatcgc gccacgggcg
     2461 tcgagcaggt tgaaacagtg cgagcatttc atgacttgct cgaatgcggg aagcgccaga
     2521 tccagcccca tcagccgctt ggcttcggat tcgtggtcgc tgaagtggcg gaaaagcatt
     2581 tcgatattcg aatgctcgaa gttgtatttt gactgctcga cctcgttctg gtggtaaacg
     2641 tcaccatagg tcacgcggta ccccggccct tcggcccagg tcaggtcaaa gacgttttca
     2701 acgccctgca ggtacatcgc cagccgttcg atgccgtagg tgatttcacc gagcaccggt
     2761 ttgcaggcga gcgagccgac ttcctggaag taggtgaatt gcgtcacttc cat
//
LOCUS       DAORPM010000285         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_12821_length_2787_cov_7.155930, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000285 DAORPM010000000
VERSION     DAORPM010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12821_length_2787_cov_7.155930"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PLD53_11190"
     CDS             complement(<1..161)
                     /locus_tag="PLD53_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRC61526.1"
                     /translation="MSLNEILAGVPLISPFIEFGFMRRALAGCLALSLGATPIGVFLM
                     LRRMSLAGDA"
     gene            complement(158..952)
                     /locus_tag="PLD53_11195"
     CDS             complement(158..952)
                     /locus_tag="PLD53_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRC61527.1"
                     /translation="MNSAQGPILRFENLTLGYNRHPAVHHLRGEVAAGSLLAVVGPNG
                     AGKSTLLKGIVGELRPMQGCVELIGLKRERIAYLTQQSTLDTSFPIVIHDFVAMGLWA
                     QFGAFRGFNRAAKQRIEEAIGAVGLSGLENRPIGQLSGGQQQRARFARVMLQDSPLVL
                     LDEPYGAIDANTVRDLAALVRHWNSEGRTIISVLHDFEHVRQEYPETLLLAREMVARG
                     STAQVLSEENLHRARHLAEVQGDFQGAAICHDNARDAHDVAEHHHG"
     gene            complement(949..1092)
                     /locus_tag="PLD53_11200"
     CDS             complement(949..1092)
                     /locus_tag="PLD53_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61528.1"
                     /translation="MPSKLNAPATSPITRPNPVICAGALSRLIWAAGALLVLWLTVHW
                     ALS"
     gene            complement(1163..2041)
                     /locus_tag="PLD53_11205"
     CDS             complement(1163..2041)
                     /locus_tag="PLD53_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRC61529.1"
                     /translation="MKRIALKIDADTCRGTLAGVPALVDLLQKHEAQASFFFSLGPDR
                     SGRESLSTSLGRYYDLRTRLYGRMLPGPDIGRRGLATMRQVSDAGHEVGIHAWDRVSW
                     EQQIEQAANPWVEAEMNKAFTRFSEIFGEPAKAHGAAGWRMNRHALRLTQRLGFSYAS
                     DCRGIHPFIPVIDGEIVVCPQIPTTLPTLDEIQVFKPGSSPDQAADRILQLTAEDPGD
                     HVFTLRAELEGMQFISVIERLISGWKDDGYQLVALRDIRSTLDVSQLPHHIVCFAETP
                     GRIGLRMMQGQVFLQN"
     gene            complement(2145..2516)
                     /locus_tag="PLD53_11210"
     CDS             complement(2145..2516)
                     /locus_tag="PLD53_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HRC61530.1"
                     /translation="MKMQLAQTAGQNTFTAYGEGYVSVNAVRHSCNLVVLPDRLIPDW
                     TKAGFETLSVGDFELLASLDTEIVLLGTGNLIRFPRPELLRPLIQAQKGLEVMDIQAA
                     CRTYNVLIGEGRKVAAGLIFS"
     gene            2666..>2787
                     /locus_tag="PLD53_11215"
     CDS             2666..>2787
                     /locus_tag="PLD53_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRC61531.1"
                     /translation="MKPVLKSSKLANVCYDIRGPVLQQAKQMEDEGHKIIKLNIG"
BASE COUNT          585 a          774 c          869 g          559 t
ORIGIN      
        1 gcatccccgg cgaggctcat gcggcgcagc atcaggaaca cgccaatcgg cgtcgcgccg
       61 agtgaaaggg ccaggcagcc ggccagcgca cgacgcatga agccgaactc gatgaaaggc
      121 gaaatcagcg ggacgccagc cagtatttcg ttcaggctca tccgtggtgg tgctccgcta
      181 catcgtgggc gtcgcgggca ttgtcatggc agattgccgc gccctggaag tcaccctgca
      241 cttcggccag atggcgcgcg cggtgcaggt tttcttcgct gagaacctgt gccgtcgaac
      301 ctctggccac catttcgcgt gccagcaaaa gcgtctccgg atattcctgg cgaacgtgct
      361 cgaaatcgtg caggacgctg atgatggtgc gtccttcgct gttccagtgg cgaaccagcg
      421 ccgccaggtc gcgcaccgta ttggcgtcga tggcgccgta gggttcgtca agcagaacca
      481 gcggcgaatc ctgcaacatg acgcgggcaa agcgcgcccg ctgctgctgg ccgcctgaga
      541 gctggccgat cgggcggttt tccagcccgc tcagtccgac ggcgccgatc gcctcctcga
      601 tgcgctgttt tgctgctcga ttgaagccgc gaaatgcgcc gaattgcgcc cacagcccca
      661 tggcaacgaa atcgtgaatg acaatgggaa aactggtgtc gagcgtcgat tgctgcgtga
      721 ggtaggcgat tctttcgcgc ttgagcccga tcagctcgac gcaaccctgc atcgggcgca
      781 attcgccgac gatacccttg agcagcgtcg attttcccgc gccgttgggg ccgactacgg
      841 ccagcaggct tccggctgcc acctcgcccc ggaggtggtg caccgccgga tgccggttat
      901 agccgagcgt cagattttcg aagcggagaa tcggaccttg tgcagaattc atgacagcgc
      961 ccaatgaacg gtcagccaca gaacaagcag cgcgcccgcg gcccagatca gacgtgacag
     1021 tgcacctgca cagattaccg ggttcgggcg ggtgatcggc gatgtcgccg gggcgttcaa
     1081 tttggatggc attgtttgag gcttttgatc gcaaggtgct gcatagtgaa acaaaggggc
     1141 gcaaacgaca aatgatacgc cttcaattct gcagaaagac ttggccctgc atcattcgga
     1201 ggccgattcg gccaggtgtt tcggcaaagc agacgatgtg gtgcggcagt tgcgagacgt
     1261 cgagcgtcga acgaatgtcg cgcaaggcta cgagttgata gccgtcgtct ttccatccgc
     1321 tgatcagccg ctcgatcacg ctgatgaatt gcattccctc cagctccgca cgcagggtga
     1381 atacatggtc gccgggatct tcggcggtga gttgcaggat acggtcggcg gcttggtctg
     1441 gagaagagcc gggtttgaac acctggattt catccagggt ggggagcgtt gtcgggatct
     1501 gcgggcagac gacgatttct ccgtcgatga ccgggatgaa ggggtggatg ccccggcaat
     1561 cactggcgta ggagaagccg agtcgctgcg tcaggcgcag ggcatgccga ttcatccgcc
     1621 atcccgcggc cccgtgcgct tttgccggtt cgccgaatat ctccgaaaag cgggtgaagg
     1681 ctttgttcat ttctgcctcg acccacggat tcgcggcttg ttcgatctgc tgttcccaac
     1741 tgacgcggtc ccaggcgtga ataccaacct catggcccgc atcgctgacc tgacgcattg
     1801 tcgcgaggcc tcggcgcccg atgtccgggc cgggcaacat tcttccatag aggcgtgtgc
     1861 gcaggtcata gtatcggccg agagaagtgg aaagcgattc gcggccactg cggtcgggcc
     1921 cgagcgagaa gaaaaagctt gcctgggcct cgtgtttctg caataggtca accaatgcag
     1981 gaacgccagc aagcgtccca cggcaagtgt cggcgtcgat tttgagtgcg attcgtttca
     2041 ttcagaggct gttctggcga taggtctgcc gttggaaatg accaggaaca caggccgtaa
     2101 ggcggcaaag aggcaacaat tcaagcttgg gttcaattgg tcgatcagga aaaaataaga
     2161 cctgcggcaa ccttgcgtcc ttcgccgatc agaacgttgt aggtgcggca ggcggcctga
     2221 atatccatca cttccagacc cttctgtgcc tgaatcagtg gccgcaggag ctcgggacgg
     2281 ggaaagcgga tcaggttacc cgtaccgagc agaacaatct cggtgtcgag cgatgcaagc
     2341 agttcgaaat ccccaacact gagggtttca aagccggctt tcgtccagtc ggggatcagg
     2401 cgatcgggaa gtacgacaag gttgcagctg tggcggacgg cattgacgct tacatagccc
     2461 tcgccgtacg cggtaaaggt attctggcca gcggtctgag caagctgcat cttcataaaa
     2521 ttgcggtgca catggcgaat aaagtacgat tatatctttt tgcctgatgc cgtttgatgt
     2581 tgagcgcaga cggtgccgag tgtagttcac aggatgtatt cggaaaggac ggcttgccac
     2641 agggaagccg cattggtaga ccactatgaa acccgtactt aaatccagca aactcgccaa
     2701 tgtctgttat gacatacgtg gtccggtgtt gcaacaggcc aagcagatgg aggacgaggg
     2761 gcacaagatc atcaagctga atatcgg
//
LOCUS       DAORPM010000286         2780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_12874_length_2780_cov_8.241101, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000286 DAORPM010000000
VERSION     DAORPM010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12874_length_2780_cov_8.241101"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLD53_11220"
     CDS             <1..255
                     /locus_tag="PLD53_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61532.1"
                     /translation="KLIRGEVVIGERRVDLSALVMPVLNVYATQDHLVPPAASKALKE
                     LCRSRDYSELSFRGGHIGIYVSARAQREIPPAIAAWLIAR"
     gene            complement(304..867)
                     /gene="phaR"
                     /locus_tag="PLD53_11225"
     CDS             complement(304..867)
                     /gene="phaR"
                     /locus_tag="PLD53_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HRC61533.1"
                     /translation="MSEPRIIKKYPNRRLYDTAVSSYITLENVKQLVLDRAVFNVIDA
                     RTNTDITRGILLQIISEQEEQGNPIFTTEVLAHIIRFYGDTLQGMMGNYLEKSLQAFV
                     DQQHLFREQMRTLIGKNPLSMMTELVEHNLSLWKSVNDRLQQYVPPGAGETPLFSPST
                     TSSPSTDPGTEPTKPGKERSGKKTKGS"
     gene            complement(934..1680)
                     /gene="phbB"
                     /locus_tag="PLD53_11230"
     CDS             complement(934..1680)
                     /gene="phbB"
                     /locus_tag="PLD53_11230"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRC61534.1"
                     /translation="MSRVAVVTGGVGGLGTAMIQALAAAGRRPVAIDYSGIKPEVVDK
                     WKADRKAEGLDVPVYLADVSNFESCAEVCKKIEAEVGPIEILVNNAGITRDAMFHRMT
                     PDQWDQVIKTNLYSVFNMCKQVYEGMTSRGWGRIVNISSVNGLRGQAGQTNYSATKSG
                     IYGFNKALAQECVKKGVTVNSISPGYMGTEMVRAIKPDVLEKVIAGIPAGRLGEPSEI
                     ARTVVFLTCDDAGYITGEDITVNGGLYYHS"
     gene            complement(1715..>2780)
                     /locus_tag="PLD53_11235"
     CDS             complement(1715..>2780)
                     /locus_tag="PLD53_11235"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRC61535.1"
                     /translation="QTGVKPEQIDEVILGQVLTAGTGQNPARTAALNAGLPIEVPCLN
                     INKVCGSGLKAVHLAYQSIRDGDNRIVIAGGQENMSLSPHLLTHSRRGTTMGDARLVD
                     SMLSEGLHCAINHYHMGITAENIAAQWAISREEQDAFAAASQDKAIAAINAGRFKDEI
                     VPIKIPQRKGDPLVFDTDEFPRAGTTAAALGKLRPAFKQDGSITAGNASGINDGAAAV
                     LVMTASAARDYGLEPLARIVAYAKAGVDPKIMGTGPIPASRACLRKAGWTPDQLDLVE
                     SNEAFAAQAISVNREMGWDTSKVNVNGGAIALGHPIGASGCRILVSLLHEMVRRDAKK
                     GLATLCIGGGQGVALAVERS"
BASE COUNT          594 a          768 c          771 g          647 t
ORIGIN      
        1 aagttgatca gaggtgaagt tgttatcggc gaacgtcgcg tggatttatc ggctctcgtc
       61 atgccggtgc tgaatgttta cgccactcag gatcatctgg taccccctgc ggcttctaaa
      121 gccttaaaag agctttgtcg gagccgggat tattcggagc tttcctttcg cggcggccat
      181 atcgggattt atgtcagcgc tcgcgcccaa cgtgaaattc ctcccgctat cgctgcctgg
      241 ctaatagcgc gctaacacac agattttaac cagtcgaaac caaagcggat tggaggcaac
      301 gcctcacgaa cctttggttt ttttaccgga ccgttcttta cccggtttgg tcggttcggt
      361 gccgggatcg gtgctgggag atgaggtcgt tgagggagaa aacagcgggg tttcaccagc
      421 ccctggaggg acatactgct gtagccggtc gttgacgctt ttccataacg agaggttatg
      481 ttccaccaac tcggtcatca tcgagagtgg atttttcccg atcagcgtcc gcatctgttc
      541 ccggaacaaa tgctgttgat caacaaaggc ttgcaaactc ttttccagat aattgcccat
      601 catcccttgc agggtatcgc catagaaccg gatgatgtgc gcaaggactt ccgtggtgaa
      661 aatcgggttg ccttgttctt cttgttcact aatgatttgc agcaaaatcc cgcgagtaat
      721 atccgtgttg gttctggcat cgatcacgtt aaagacggcg cgatccaaca ccagttgctt
      781 gacgttttca agggtgatgt agctactcac cgctgtgtcg taaaggcggc gattgggata
      841 tttcttgatg atgcggggtt cgctcatgac aggtcgggtg caaaaaacga tggcggcgcc
      901 ggttcagtac cgccgccgca caacgtttag tgattaactg tggtagtaga gaccgccgtt
      961 gaccgtaata tcttcaccgg tgatatagcc agcgtcatca caggttagga acacaacagt
     1021 acgggcaatt tcggaaggct cacccaggcg tccggccggg ataccggcga tgactttctc
     1081 cagcacgtcc ggcttgatgg cgcgtaccat ttcggtcccc atgtaacccg gagaaatgga
     1141 gttgaccgtc acacccttct tgacgcattc ctgcgccagc gctttgttaa agccgtagat
     1201 acccgacttg gtggcggaat agttggtctg gccagcctga ccccgcaggc cattgactga
     1261 cgagatgtta acgatacgac cccaaccccg cgaggtcatt ccttcataaa cctgcttgca
     1321 catgttaaag acgctgtaca agttggtctt gatgacttga tcccactgat caggggtcat
     1381 ccgatggaac atcgcgtcac gggtaatacc ggcattgttg accaggattt cgatggggcc
     1441 aacttctgct tcgattttct tgcagacttc agcgcaagat tcaaagttgg agacatcggc
     1501 caggtaaacc ggaacgtcaa gcccttcggc tttgcgatcc gctttccact tgtcgaccac
     1561 ttccggcttg atgccgctgt aatcgattgc gaccggacgc cgacctgcgg cggccagcgc
     1621 ctggatcatc gccgtgccca agcccccaac accaccggta acgactgcaa ctcgagacat
     1681 taaacttcct cctcaattat cgtgtaggta tgaactaact gcgttcgaca gccagggcga
     1741 cgccttgacc gccgccgatg cacagggtcg caagaccctt tttggcgtca cggcggacca
     1801 tttcatgcag caaggagacc aggatgcggc agccggaagc gccgatcgga tggcccagtg
     1861 ctatggcacc gccattgaca ttcactttgc tggtgtccca gcccatttca cggttgacgg
     1921 agatcgcctg cgcggcgaac gcttcattgg attccaccag atcaagctga tcaggagtcc
     1981 aaccggcttt gcgcaagcag gcgcgtgacg cagggatagg accggtgccc atgatctttg
     2041 gatcaacgcc cgccttggca taagcaacga tgcgcgccag cggttcgagg ccataatccc
     2101 gtgcggcgct ggcggtcatc accaacacag cggcagcgcc atcattgatg ccggaggcgt
     2161 tgccagcggt aattgagcca tcctgcttaa acgcgggtcg tagcttgccc agcgcagcgg
     2221 cagtggtgcc cgcgcggggg aactcgtcgg tatcaaatac cagcggatca cccttgcgct
     2281 gtggaatttt gatcgggacg atttcgtctt tgaaccggcc agcgttgatg gccgcaatgg
     2341 ctttatcctg ggaggccgct gcaaaggcgt cctgttcctc acgggaaata gcccattgag
     2401 cggcgatatt ttcagcggtg atgcccatat gatagtgatt gatggcgcaa tgcagccctt
     2461 cactgagcat ggaatcgacc agccgggcat ctcccattgt tgtaccacgg cgcgaatggg
     2521 tcagcaggtg gggcgagagg ctcatatttt cctgaccgcc agcgatgacg attcggttat
     2581 cgccgtcgcg aatcgactga taggccagat gaaccgcctt gaggccgcta ccgcagactt
     2641 tattaatatt caggcaggga acttcaatag gcaaaccagc gtttagcgca gcggtgcgcg
     2701 ccgggttctg gcccgtacca gcggttagca cctggcctaa tatgacttca tcaatctgct
     2761 caggcttaac cccggtttgg
//
LOCUS       DAORPM010000287         2779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_12882_length_2779_cov_8.174009, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000287 DAORPM010000000
VERSION     DAORPM010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12882_length_2779_cov_8.174009"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..2379
                     /locus_tag="PLD53_11240"
     CDS             82..2379
                     /locus_tag="PLD53_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRC61536.1"
                     /translation="MITGLRPVPDARKQVMRDPQVQACWIEVDCGFQPVADVFEECVS
                     EALSAFSSAQMRDYLDAVRFLGKLGRGPDPMLAFLEEWPSVVASIGGDSDLLPRVMDF
                     VRVLQKSPNSKAITPMLQTLAAVARQLHSPDQLQRYLGIAQDLMIRTSGSIHGHHTTF
                     PSPGLADFFAQAPYLTRLLPMAGLKNWVDYGIHNYASHPERQVDYFKLESADSRAVLQ
                     RERHGTLFADVERKLDLYLRALWNDSDLLVPYSTAFDELRQPVPYYDKLGLRVPDVFD
                     DINGVSGLDRYRAVLAHMVGHRRWSKPLIADNWSPFQRMAVEFFEDGRVDNLLIREYP
                     GLRRILLALHPKPVETDCDPQTTSCLRHRLAMLSRALIDPAHGYTDPDLLEFVRRFNA
                     LIAESESTTREIAELALAYVTRTRRQSDQFAKVHFQNTVIDYRDDNRQMWQFIEEGDE
                     ESAFDEERKLQPTETVQGLPPRHYPEWDYNSQTYRPDWASVYETLHPGGNAADIDSLL
                     NKHSALAKRLKRLFDLLKPQDKVRIRYQEEGSELDLDIAIRSLIDFKSGVTPDSRINM
                     SHRTNGRDIAVMLLLDLSESLNETVAGSGQSILQLSQEAVSLLACAVDRLGDPLAIAG
                     FHSNTRHDVRYQHIKGYSEKWSDEVKARLAALDAGYSTRMGAAIRHAAHYLENQKADK
                     KLLLILTDGEPADVDTSDPHTLIEDARQAVKELDTMGIYTHCINLDAHADEYVRQIFG
                     QRFTVIDNIQRLPERLPQLFLALTK"
     gene            2520..>2779
                     /locus_tag="PLD53_11245"
     CDS             2520..>2779
                     /locus_tag="PLD53_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61537.1"
                     /translation="MRYSLLCLVFFSALAMAEAVPSNFNQAEFEKRFRAADKNKDGKL
                     SRTEAYEEFPRMPEFFDEIDTNKDSSITLIEVNKAMERRVNAA"
BASE COUNT          602 a          889 c          740 g          548 t
ORIGIN      
        1 tcgatcacgc catcgatgcc acgtttgcct gatttttcca ttccggccgg tcggcggcgc
       61 gcactcgcaa cataacagca catgattacc ggtctacgac ccgtacccga cgcccgcaag
      121 caggtcatgc gtgacccgca agtccaggct tgctggatcg aggtcgattg cggcttccag
      181 cccgtggccg atgttttcga ggagtgcgtc agcgaagcct tgtcggcttt ttccagcgct
      241 cagatgcggg actaccttga cgccgtgcgc ttcctcggca aactgggccg cgggccggat
      301 ccgatgctgg ccttccttga agagtggcca agcgtcgtcg ccagtatcgg cggcgatagc
      361 gacctcctgc cgcgagtgat ggatttcgtg cgcgttctac aaaagtcgcc gaacagcaag
      421 gccatcacgc ccatgctgca aacacttgcc gcggtggccc gccagctcca ctcgcccgat
      481 cagttgcagc gctatctcgg tattgcgcag gacctgatga tccgcacctc cggctccata
      541 cacggccacc acaccacgtt tcccagccct ggtctcgcgg acttcttcgc tcaggccccc
      601 taccttaccc ggctgttgcc catggccggg ttgaagaatt gggtggatta cggcatacat
      661 aactacgcca gccacccgga acggcaggtc gactacttca agctggaatc ggcagacagc
      721 cgtgcggtac tgcagcgcga acgccacggc acgctgttcg ccgacgttga aagaaagctg
      781 gatctttacc tgcgcgcgct gtggaatgac agcgatcttc ttgtgcccta ttcgaccgct
      841 tttgacgaat tgcgccagcc ggtaccctat tacgacaagc tgggcctgcg cgtgcccgat
      901 gtcttcgatg acatcaatgg cgtcagcgga cttgaccgct accgcgccgt tctcgcccac
      961 atggtcggcc atcgccgctg gagcaagccg ctgatcgccg acaactggag cccttttcag
     1021 cgcatggcag tggagttttt cgaggacggc cgcgtcgata acctgctgat tcgcgaatac
     1081 cccggcttgc gccgtattct tctggcactg catcctaaac cagtcgaaac ggattgcgat
     1141 ccgcagacca cgtcctgcct gcgccatcgg ctggccatgt tgtcgcgcgc gctgatcgac
     1201 cctgcgcacg gctacaccga ccctgatctg ctcgagttcg ttcggcgctt caacgcgctg
     1261 atagccgaaa gcgaatcgac tactcgtgag atcgccgaac tggcactcgc ctacgtcacg
     1321 cggacacgcc gtcagagtga ccagttcgcc aaagtgcact tccaaaacac tgtcattgac
     1381 taccgtgatg acaatcgcca gatgtggcaa ttcatcgagg agggcgatga ggaatcagcc
     1441 ttcgacgaag agcgcaagct gcagccgacc gaaacagtgc aaggattgcc acctcggcac
     1501 tatcccgaat gggactacaa cagccagacc taccgcccgg attgggccag cgtttatgaa
     1561 acgctgcacc ccggcggcaa tgccgcagac atcgacagtc tgttgaacaa gcacagcgca
     1621 ctggccaaac gactgaaacg tctgttcgat ctgctcaaac cgcaggacaa ggtacgcatc
     1681 cgttatcagg aagaaggcag cgagctcgat cttgacatcg ccatccgctc gctgatcgac
     1741 ttcaagagcg gcgtcacccc cgattcgcgc atcaatatga gccatcgtac caatggccgc
     1801 gacattgcag tcatgctgct actcgatctc tccgaatcgc tcaacgagac ggtcgccggc
     1861 agcggacaga gcatcctgca actgagccag gaagccgtgt ccctgctcgc ctgtgcggta
     1921 gaccggctgg gcgatccact ggccatcgcc ggctttcact cgaacacacg gcacgacgta
     1981 cgctatcagc acatcaaggg ctacagcgag aagtggagcg acgaggtcaa agcccgtctt
     2041 gccgcgctgg atgccggcta ctcgacacgc atgggcgcgg ccatacgcca tgccgctcac
     2101 tacctggaaa accagaaggc cgacaagaag cttttgctga tcctcaccga tggcgaaccc
     2161 gccgacgtgg acacctccga cccgcacacc ctgatcgaag acgcacggca agccgtgaag
     2221 gaactcgaca ccatgggcat atatactcat tgcatcaacc ttgacgcgca tgccgacgaa
     2281 tacgtgcgcc agatattcgg ccagcgcttc acggtaatcg acaacatcca gcgtctgccg
     2341 gagcgcctgc cccagctgtt ccttgccttg acgaagtagg cgtcatcatc cataacgcgt
     2401 cggatgaact ccagtcattc gattccgtct tcgctcatct caagctgttc ctccctcgat
     2461 tgccgcacga ggtgatttgg tgcatattcc aaagccggtc atcaatccag gaaaatgcaa
     2521 tgcgctattc gctgctttgt ctcgtgtttt tctctgccct tgctatggct gaagccgtgc
     2581 catccaattt caatcaggct gaattcgaga aacgatttcg tgcggctgac aaaaacaagg
     2641 acggcaaact gtcgcgcacg gaagcctatg aggaatttcc acgcatgccc gagtttttcg
     2701 acgagatcga cacgaacaag gacagcagca ttaccttgat tgaagtcaac aaagccatgg
     2761 aacgccgagt aaatgccgc
//
LOCUS       DAORPM010000288         2756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_13065_length_2756_cov_9.524991, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000288 DAORPM010000000
VERSION     DAORPM010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13065_length_2756_cov_9.524991"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLD53_11250"
     CDS             <1..653
                     /locus_tag="PLD53_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRC61538.1"
                     /translation="YALMAQAQALGIEPVLLGIARDDPEELAQFVRRGLLFDVLLISG
                     GSSVGLHDHVRPTLEALGVDMHFWRVEMRPGHPLAFGTTASTMVFGLPGNPVSSMVCF
                     EQFVLPALRRMMGHSHLFRRTIKARLTHPVKIRPGRTEFIRVVLERNESSRLPGSYVA
                     SSTGTQSSGALLSMALADGLLVVPSTSSGLAAGESAVIQLLDGAVFQDEPGFEGGT"
     gene            650..1189
                     /gene="mog"
                     /locus_tag="PLD53_11255"
     CDS             650..1189
                     /gene="mog"
                     /locus_tag="PLD53_11255"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001954004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HRC61539.1"
                     /translation="MSTARIGILTVSDRASRGVYEDKGGPAIQAWLTRVLTSPWEAVA
                     RVIPDEQPQIEDALRELSDIEGCSLIVTTGGTGPALRDVTPEATEAVCTKMMPGFGEL
                     MRTASLKFVPTAILSRQTAGIRGKSLIVNLPGKPSAIDDCLNAVFPAVPYCLDLIEGA
                     RLETDPQHCTAFRPGQGKK"
     gene            1207..2007
                     /gene="modA"
                     /locus_tag="PLD53_11260"
     CDS             1207..2007
                     /gene="modA"
                     /locus_tag="PLD53_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRC61540.1"
                     /translation="MVRIPTNKWIAPLALVLFLVVQLTLPARAEEKITLAAAADLKYA
                     LDEVVVLFIRENPDARVETVYGSSGKFHAQIRQGAPYDLYFSADIAYPRALVEAGLAA
                     SEVQPYGIGRIVLWGTTRDAAKMTLMALADLADPAITRIAMANPRHAPYGQRAEEALR
                     SAGIWERVKPRLVFGENVAQSAQFVQTGNAQFGIIALSLALSPELSEHGSYVLIPETL
                     HRPLEQGFIITRRAAGKPLAQRFARFMKGTDARATMTRYGFVLPGEAK"
     gene            2012..2695
                     /gene="modB"
                     /locus_tag="PLD53_11265"
     CDS             2012..2695
                     /gene="modB"
                     /locus_tag="PLD53_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRC61541.1"
                     /translation="MGSYLSAGDISAIWLTVKLATLVTLILLVIGTPLAWWLARTRSS
                     MKGAIGAVVALPIVLPPTVLGFYLLLAMGPHGPLGQLTKSLGLGLLPFSFAGLVIASV
                     FYSLPFVVQPLQNAFENIPERALETAATLRASPLDRFFSVVLPLAKPGYLTALVLGFA
                     HTVGEFGVVLMIGGNIPEQTRVVSIQIYDHVEAMDYAHAHGLSAGMLAFSFLVLLVLY
                     TLNPTGRRK"
BASE COUNT          490 a          847 c          845 g          574 t
ORIGIN      
        1 gttacgcgct gatggcgcag gcgcaggctc tggggatcga gccggtcttg ctcggcatcg
       61 cgcgcgacga ccccgaagaa ctggcgcaat ttgttcgccg cggcctgttg ttcgatgtgc
      121 tgctgatttc cggcggctct tccgttggcc tacatgatca tgtccgacca acgcttgaag
      181 cgctgggcgt tgacatgcat ttctggcgtg tggagatgcg gcccggacat ccgctggcct
      241 tcggcaccac cgcctcgacc atggtctttg gcctgccggg caacccggtg tccagcatgg
      301 tgtgtttcga gcagttcgtc ctgccggcct tgcgccggat gatggggcat tcacatctgt
      361 tccggcgcac gatcaaagcg cgcctgactc acccggtcaa gattcgtccg ggcagaaccg
      421 aattcatccg cgtcgttctg gagcgcaatg aatccagcag gctgccaggt tcctatgtcg
      481 ccagttcgac cgggacgcaa agcagcggcg ctttgctgtc gatggccctg gccgacggtc
      541 tgctggttgt gccctcgaca agctcgggcc tggcagcggg cgaatcggcc gtgattcaac
      601 tccttgacgg cgctgttttc caggacgaac cgggattcga gggtggcaca tgagcacggc
      661 tcggatcggc attctgacgg tctccgatcg cgccagccgg ggtgtttatg aagacaaggg
      721 cgggccggcg atccaggcct ggctgacgcg tgtgctgacc agcccgtggg aggcagttgc
      781 cagggtcatt cccgacgagc aaccgcaaat cgaggacgcg ttgcgcgaat tgagcgatat
      841 cgagggatgc agtttgatcg tgacgaccgg aggcaccggt cccgcgctac gcgacgttac
      901 cccggaagcg acagaagccg tgtgcaccaa gatgatgccc ggttttggcg agctgatgcg
      961 caccgcctcg ctgaagttcg ttccgacagc gatcctgtcc cgacagacag cgggtattcg
     1021 cggcaagagc ctgatcgtca acctgcctgg caaaccgtcg gcgattgacg attgcctgaa
     1081 tgccgtattc ccggcggtgc cctattgcct tgatctgatc gaaggcgcac ggcttgaaac
     1141 cgaccctcag cactgcacgg cgtttcgccc gggccaaggg aagaagtgat ccggaaagcg
     1201 atccgaatgg ttcgaattcc gacgaacaaa tggatcgcac cgcttgctct ggtgctgttt
     1261 ctcgtcgtgc agttgacgtt gccggcacgc gccgaagaga aaatcaccct cgctgcggcg
     1321 gctgacctca aatacgccct ggacgaagtt gtcgtcctgt tcatcaggga gaacccggac
     1381 gcccgggtcg aaaccgttta tggttcatcc ggcaaatttc atgcgcaaat ccggcagggc
     1441 gctccttacg atctctattt ttcggcagat attgcctatc cacgcgcgct ggtggaagcc
     1501 ggtctggccg cttccgaagt acaaccctac ggcatcgggc ggatcgtgct gtggggaacg
     1561 acacgcgatg ccgcaaagat gacgctcatg gcactcgccg accttgccga tccggccatc
     1621 acaagaatcg cgatggccaa tccacggcat gccccctatg gccagcgtgc ggaagaggcg
     1681 ctgaggtcag caggcatttg ggagcgggtc aagccccgac tggtttttgg cgaaaacgtc
     1741 gcacagtcgg cgcagttcgt acaaaccggc aacgcgcaat tcggcatcat cgccttgtcg
     1801 ctggcgctca gtcccgagct gtccgaacat ggcagttatg tgctgatacc cgaaaccttg
     1861 catcgtccgc ttgagcaggg cttcattatt acccggcgag ccgccggcaa gccattggcg
     1921 cagcgtttcg cgcgcttcat gaagggtacc gatgcgcgcg ccaccatgac gcgctacggc
     1981 ttcgtgctgc cgggcgaagc aaaatagcgc gatgggatcc tacctttcgg cgggcgacat
     2041 ttcggcgatc tggctgactg tcaaactcgc cacgctggtc accttgatac tgctcgtcat
     2101 cggcacgccg ctggcctggt ggcttgcacg cacccgatcc agcatgaagg gtgccatcgg
     2161 agcggtagtc gcgctaccga tagtgctacc gcctaccgtg ctcggtttct atctcctgct
     2221 ggccatggga ccacacggcc ccttggggca gctcacgaag tcgctgggcc ttggcctgtt
     2281 gccgttcagc tttgccgggc tggtgatcgc ttcggtcttc tattcgttgc ccttcgttgt
     2341 acagcccttg cagaacgcct ttgaaaacat ccccgaacgc gccctggaaa ccgccgcgac
     2401 actgcgagcc tcgccgctcg accgtttctt cagcgtggtt cttccgctgg ccaaaccggg
     2461 ctatctgact gcgctggtgc tcggcttcgc ccacacggtc ggcgagtttg gcgtcgtgct
     2521 gatgatcggc ggcaacattc cggagcaaac ccgggtggtg tcgatccaga tctacgatca
     2581 tgtcgaggcg atggactatg cacacgccca cggcttgtcg gcgggcatgc tggcgttctc
     2641 ctttttggta ttgctggtgc tgtatacctt gaacccgact ggcaggcgga aatgatgccg
     2701 gcaagcaacg acgccaagaa tgtcattcgc gcgcgcttcc agctcgtcta tccgga
//
LOCUS       DAORPM010000289         2751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_13097_length_2751_cov_18.469955, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000289 DAORPM010000000
VERSION     DAORPM010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13097_length_2751_cov_18.469955"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLD53_11270"
     CDS             complement(<1..812)
                     /locus_tag="PLD53_11270"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRC61542.1"
                     /translation="MTQPSHYFKRHFPTFLLTVALLLLAQAPGAFSAELPPAVTHALQ
                     EAGIPLRSVAVVVQGVDADRPLIRHNALQAMNPASAMKLVTTYAALELLGPAYTWKTE
                     ALADSLAIKGQLSGNLYLRGSGDPRLALEQFWLLLRQLRARGVTNIGGDLILDRSAFA
                     LPIHNPAEFDNEPLRPYNAGPDALLVNLKSLRLTLTPDAAQNAVGVVAETPSEDLRIE
                     NRLALTRDACGDWRERIKVKVTDESIELMGSLSASCGDKALNLSPWPADVQV"
     gene            835..1434
                     /locus_tag="PLD53_11275"
     CDS             835..1434
                     /locus_tag="PLD53_11275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRC61543.1"
                     /translation="MREKQLAKWVRSVMKEQRRHQRIRFNVLPQVRIGQYGVVGTGEL
                     ENISLGGLMLRTELLLKVGQALGCEFVIFDSPLIDMTAVVASKIGDRYGVRFQAGPIS
                     EALIQNAIDTALTTGQASVLSINDLQGRKVMRIAGGLNGGLRNDFMHSLTKMGVDEMD
                     LSGVTEIDSAGLDLCRIAVEQHRVGIVSPSSCVRALMAA"
     gene            1506..1820
                     /locus_tag="PLD53_11280"
     CDS             1506..1820
                     /locus_tag="PLD53_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HRC61544.1"
                     /translation="MVDDLVARVEANPKYQKLVSARSSYSIFMTSLVMLVYFGFILLI
                     AFDKELLATKLGPGMVTSLGIPLGIGVIVITIILTNIYLRRANSEFDRLNADIIKEAK
                     RK"
     gene            1862..>2751
                     /gene="actP"
                     /locus_tag="PLD53_11285"
     CDS             1862..>2751
                     /gene="actP"
                     /locus_tag="PLD53_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434523.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRC61545.1"
                     /translation="MLAASFVFAANADFGQTTRQATNWVAISMFIGFVTITLFITRWA
                     ANTTRTAADFYTAGGGVTGFQNGLAISGDYMSAASFLGISGLVFANGFDGLIFSIGWL
                     VGWPVLTFLMAERLRNLGRFTFADVASYRFAQTPIRVFAACGTLAVVAFYMIAQMVGA
                     GQLIKVLFGLEYHFAVIIVGALMICYVLFGGMMATTWVQIVKSVFLLCGASFMALAVL
                     WHFHFNPELLFAKAVEIHSEHDAIMAPGALIKDPISAISVGVTLMFGTAGLPHILMRF
                     FTVSSAKEARKSVGWATTWIG"
BASE COUNT          540 a          735 c          828 g          648 t
ORIGIN      
        1 tcgacctgca catccgctgg ccacggcgaa agattcagcg ccttgtcacc acaactcgcc
       61 gagaggctgc ccatgagttc gatgctttcg tcggtgacct tgaccttgat gcgttcacgc
      121 cagtcgccgc aggcgtcccg ggtcagtgcc agacggttct cgatacgcag gtcttcactc
      181 ggtgtttcgg cgaccacgcc aacggcattt tgtgctgcgt caggggtcag ggtcagacgc
      241 aggctcttga ggttgaccag caaggcgtcc gggccagcgt tgtagggacg cagcggttcg
      301 ttgtcgaact cggccgggtt gtgtatcggc aaagcgaagg cgctgcggtc gagaatcagg
      361 tcgccgccaa tattggtgac gccgcgcgcg cgcaactggc gtagcagtag ccagaactgc
      421 tcgagggcaa gccgcgggtc gccgctgccg cgcaggtaga ggttgccgct cagttgtccc
      481 ttgatggcca gtgaatccgc cagagcttcc gtcttccagg tgtaggccgg accgagcagt
      541 tcaagcgcag cgtaggtggt aaccagcttc atcgccgacg ccggattcat tgcctgcagg
      601 gcgttatgcc ggatgagcgg acggtcggcg tcaactcctt gcacgacaac agccacactg
      661 cgcagaggaa taccggcctc ttgcaaagca tgggtgaccg caggcggtaa ttcggcgctg
      721 aaagcgccag gcgcctgtgc cagcaaaagc agagcaactg tcagcaggaa cgttgggaaa
      781 tgccgtttaa agtaatggct tggttgcgtc atggtggagg gatgcatgta agatgtgcgg
      841 gaaaaacaat tagcaaaatg ggtcaggagc gtcatgaaag aacaacgcag acatcagcga
      901 attcgtttca acgtcttgcc acaggttcgt atcgggcaat acggagtcgt cggaactggc
      961 gaactggaaa atatttcact cggcgggttg atgttgcgta ctgagctttt gctgaaagtg
     1021 ggtcaagcac tcggttgcga gtttgtcatc ttcgattcgc cgctcattga tatgactgcc
     1081 gtggtcgcaa gcaagatcgg tgaccggtat ggtgttcgtt ttcaggccgg tccaatcagc
     1141 gaagcgctga ttcagaacgc gattgacact gccctgacaa ccggccaagc ctcggtgctg
     1201 agtataaatg atctgcaggg gcgcaaggtg atgcgtattg ccggcgggtt gaacggtggt
     1261 ctgagaaacg atttcatgca tagcctgacc aaaatgggtg tcgacgagat ggatctttct
     1321 ggtgtgaccg agatcgatag cgccggtctc gatctatgcc ggatcgcagt tgagcaacac
     1381 cgggtcggga tcgtgagccc gtcgtcttgt gtacgagcgc tgatggcagc ttgaatgggt
     1441 ggtccagtcg cctgtttcga agtgggtcgc aattgcgcga ataactactg cttgaggagt
     1501 caaagttggt tgacgatttg gtcgcacggg ttgaggcgaa tcccaagtac cagaagctgg
     1561 taagtgcacg aagttcctac agcatcttca tgacttctct cgtcatgctc gtttatttcg
     1621 gatttatcct tctgatcgcc tttgacaagg aattgctggc caccaagctc ggtcccggta
     1681 tggtgacctc gctgggtatt ccgcttggca tcggggtgat cgtgatcaca atcattctta
     1741 ccaacatcta tctgcgccgt gccaattcgg agtttgaccg gctaaacgcc gatatcatca
     1801 aggaggcgaa gcggaaatga accgcaagcc ggtgcgcgac agcacatgtt ttctgctcgg
     1861 catgctggcc gcaagtttcg ttttcgcagc caacgccgat ttcggacaga caacccgtca
     1921 ggccaccaac tgggtcgcca tctcgatgtt catcggtttt gtaacgatta ccctgttcat
     1981 tacccgatgg gccgcaaaca caacccggac ggcagcagac ttctacactg ccggcggcgg
     2041 cgttaccggt ttccagaacg gtctggcgat ttctggcgac tacatgtctg ccgcatcctt
     2101 tctcggtatt tccggtctgg tgtttgccaa tggtttcgac ggcctgatct tttctatcgg
     2161 ctggctggtc ggttggcctg tactcacctt cctgatggca gagcgtttgc gcaatctcgg
     2221 caggtttacc tttgccgatg tcgcttccta ccggtttgcg caaaccccga tccgtgtatt
     2281 tgcggcatgc ggcacactgg ccgttgtggc cttctacatg atcgcccaga tggtcggtgc
     2341 cggtcagttg atcaaggtgt tgtttggcct cgaataccac ttcgcggtga ttatcgtcgg
     2401 agcgctgatg atctgctacg tgttgttcgg cggcatgatg gccacgacct gggtacaaat
     2461 cgtcaagtct gtttttctcc tctgcggtgc aagtttcatg gcgttggccg tgctctggca
     2521 tttccacttc aacccggagc tgttattcgc caaggccgtc gagatccaca gcgaacacga
     2581 tgccatcatg gcgccagggg cgctgatcaa ggatccgatc tcggccatct cggtcggcgt
     2641 gaccttgatg ttcggcacgg cgggtctgcc gcacatcctg atgcgtttct ttaccgtgtc
     2701 gagcgccaag gaggcgcgca agtcagtagg ctgggcaacg acctggatcg g
//
LOCUS       DAORPM010000290         2739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_13195_length_2739_cov_7.831222, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000290 DAORPM010000000
VERSION     DAORPM010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13195_length_2739_cov_7.831222"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLD53_11290"
     CDS             <1..435
                     /locus_tag="PLD53_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HRC61546.1"
                     /translation="ALNNLACIDRNVADTLCRAIRAIDRNLILLAPAASQLLDAGRAA
                     GLPVVEEIFADRAYLPDGQLVPRARPDAMIHGAEACLEHTLSMLEAGALIAVDGTRIP
                     TLIGSICVHGDSPGAVGIARHLRRELGARGYRFLELPKISAG"
     gene            691..1575
                     /locus_tag="PLD53_11295"
     CDS             691..1575
                     /locus_tag="PLD53_11295"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRC61547.1"
                     /translation="MTTPMYQSSIPSLPLLNRGKVRDIYAVDDQHLLIVTTDRLSAFD
                     VVLPTPIPGKGEVLTRISDFWFSRLGHLIPNQLTDIKPESVVAKGEVDQVAGRGIVVK
                     KLAPLPIEAIVRGYLVGSGWKEYKASSSVCGLPLPAGLREADKLPEPLFTPSTKAAAG
                     AHDENISYEEAEGLLGKALAAQVRDACIALYKEAAAYAATKGIIIADTKFEFGQDAQG
                     KLYLIDEALTPDSSRFWPADQYQPGSNPPSFDKQYVRDWLEESGWNKQDPGPELPPEI
                     AAKTSEKYREALIRLTEA"
     gene            1575..2216
                     /locus_tag="PLD53_11300"
     CDS             1575..2216
                     /locus_tag="PLD53_11300"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRC61548.1"
                     /translation="MMNHIEKLIEGFRRFRNNNYTQNHALFERLAKQGQSPKTIVVGC
                     CDSRVDPAIVTDCDPGDLFIIRNVANLVPPYETSGTYHGTSAALEFGVRHLQVENIIV
                     LGHCQCGGIRALMSQTPGVESQDGFISSWMKVASHARNRVLSRKHEESLEKQSRACEQ
                     EAILVSLDNLLTFPWILERVAQKKLALHGWYFDMEEGDLLRYDPDSNRFEPLS"
BASE COUNT          623 a          793 c          762 g          561 t
ORIGIN      
        1 gcactgaaca atctcgcctg tatcgaccgc aatgtcgctg acacgctttg ccgcgcgatt
       61 cgggcaatcg atcgaaatct gattctgctg gctccggccg catcgcagtt gctcgacgct
      121 gggcgcgccg ccggtttgcc ggtcgtggaa gagattttcg ccgatcgggc gtatctgccc
      181 gatggccaac tggtgccgag ggcacggccg gacgccatga tccacggcgc cgaagcctgc
      241 ctcgagcata cattgtccat gcttgaagcg ggagcgttga tcgccgtcga tggcacccgt
      301 atcccgacct tgatcggaag catctgcgtg catggcgaca gccctggcgc tgtaggaatc
      361 gcgcgccact tgcgccgcga gttgggtgct cgggggtacc ggttcctgga actgccgaaa
      421 atatcggcag gatagataat cgcctttgcc aaatttcgcg ggccgccacc cagccaagca
      481 cggctttgcc ggcaagaaac agagcttgcg ggccgatttg gagttacgat agaaggtgct
      541 ttactcgcgc actccacgcg cccggacgtg ccgctagttg accctcacta tgcaccaacc
      601 tgctggccgt ttccgttatg cgagttcaaa ctgatggccc acggaataat cacgcaacac
      661 attgtaaaca cgtaactatg gaaatatttt atgaccacgc cgatgtacca atccagcatc
      721 cccagcctgc ccctgcttaa tcgtggcaag gtgcgcgaca tctatgccgt agacgaccag
      781 cacttgctga tcgtgaccac cgaccgcttg tcagccttcg acgtggtact ccccacgccg
      841 atccccggca aaggggaagt gttgacccgc atctccgatt tctggttttc cagacttggt
      901 caccttattc ccaaccagtt gaccgacatc aaaccggaat cggtggttgc aaagggtgag
      961 gtcgaccagg tagcggggcg agggatcgtg gtgaaaaaac ttgccccctt gccgatcgag
     1021 gccatcgtgc gcggctatct ggtcggctcc ggctggaagg aatacaaggc gagcagcagc
     1081 gtttgcggtc ttcccctgcc agccggactg cgtgaagccg acaaactgcc ggaaccgcta
     1141 ttcaccccct ccaccaaagc ggcagcaggc gcccacgacg aaaacatcag ctacgaagag
     1201 gctgagggac tactcggaaa ggcactggct gcccaggtac gcgacgcatg tatcgcccta
     1261 tacaaggaag ccgctgccta cgccgcaacc aaaggcatca tcatcgctga caccaagttc
     1321 gaattcggcc aggacgctca aggaaagctg tatctcatcg atgaggcact gaccccggat
     1381 tcctcgcgtt tctggcccgc cgatcagtac cagcccggca gcaatccgcc cagctttgac
     1441 aagcaatacg ttcgcgactg gctggaagaa agtggctgga acaaacaaga tcccggcccg
     1501 gagttgccgc cggaaatcgc cgccaaaacc agcgaaaaat atcgcgaagc gctaatacgc
     1561 ctgacggaag cttgatgatg aaccatatcg agaaactgat tgagggtttc aggcgctttc
     1621 gcaacaacaa ttacacgcag aatcacgcac tgttcgaacg cctcgccaaa cagggccagt
     1681 cacccaaaac gatcgtggtc ggctgttgcg attccagggt ggaccccgcc attgtgaccg
     1741 actgcgatcc aggcgacttg ttcatcattc gcaatgtggc gaatctggtg cccccttacg
     1801 aaaccagcgg cacctaccat ggcaccagcg ctgcgctcga gttcggggtg cgtcatctgc
     1861 aagtagagaa catcattgtt ctgggacact gtcaatgtgg cggaatacgt gcgttgatga
     1921 gtcagacgcc gggagtcgaa tctcaagacg ggtttatttc aagctggatg aaagtcgcca
     1981 gccatgcgcg caaccgcgtt ctatccagaa agcacgaaga atcgctggaa aaacaatcgc
     2041 gtgcctgcga acaggaggct atcctcgtct ccctcgacaa cttgctgacc ttcccctgga
     2101 tcctggaacg cgtcgcgcag aagaagctcg ccttgcatgg ttggtatttc gatatggaag
     2161 agggcgattt actgcgttac gaccccgata gcaatcggtt tgaaccgctt tcctgacgta
     2221 ggaagaattg cctgcagcgc catgacgcga accggggaat ctggcatgca gaatcggcga
     2281 tgcgaacaag cctgatcaga gtgtttgcag tagacgacaa ctttttcgcc attgaaccta
     2341 gcattttcgg ctgcatgaac tagatttata gagcgtcgcg atctgcggtg agtcgggcgc
     2401 gttgatgttg ctccaaccgt gttttaagga gacttcatgg ttctatgctt ttaccgcatt
     2461 ttgagatgat catgtagcac cctgacggcg aaaagccgat cacttcggcc tggagtgccg
     2521 gagtaagagt aacgaaggcc cacgcaatac aaagcgtggg ccttttttcg agcacctgta
     2581 ctccgcgatt ggcaatcaac gctcattgcc cgctgcagca tctagcgacg attcatgatg
     2641 ggcttacggc tcttttgact gcctcagttc gacccattgc cgatggtggc ggaaatcgga
     2701 agcggccatt cgcggcgaag gtcggaatcg ggaaagctg
//
LOCUS       DAORPM010000291         2730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_13261_length_2730_cov_16.451589, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000291 DAORPM010000000
VERSION     DAORPM010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13261_length_2730_cov_16.451589"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLD53_11305"
     CDS             <1..378
                     /locus_tag="PLD53_11305"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942804.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRC61549.1"
                     /translation="SRWQRDLTDSTVLRNMGVALGYTLLAYDSLLRGLNKLEVNPDCL
                     RDDLDANWELLAEPIQTVMRRYGVANPYERLKELTRGNRISREGIQAFVQALDIPQTA
                     RDELLRLTPWDYTGKAAELARRI"
     gene            378..668
                     /locus_tag="PLD53_11310"
     CDS             378..668
                     /locus_tag="PLD53_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61550.1"
                     /translation="MVDIPHKVMVCPGCAREVPSGAPYCPYCCGEDGREGALRRGGFI
                     GGIFGLMAGGLAASVWFAFIGPEHATWNATLGMVFGGVIAGLVLGLIHQRKR"
     gene            738..1058
                     /locus_tag="PLD53_11315"
     CDS             738..1058
                     /locus_tag="PLD53_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HRC61551.1"
                     /translation="MSQFAANLKKLPGISHLAALNLLDAEGRVLVAIENKAGSQGSLA
                     VYNHLAQTFGAITPDAARKGLELFAEHTEDARINPGKHPNIDRLLGLIESKETLRIKH
                     VFAV"
     gene            complement(1079..2023)
                     /gene="secF"
                     /locus_tag="PLD53_11320"
     CDS             complement(1079..2023)
                     /gene="secF"
                     /locus_tag="PLD53_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRC61552.1"
                     /translation="MEFFRIKKDIPFMRYALVFNVISLLTFLFSVFFLFYNGLHLSVE
                     FTGGTLIEVNYAQAANLEQIRAGLGKAGYNDTTVQNFGSSRDVMIRLPLKADQNTAKI
                     GEAVMSALDAEAPGATLRRVEFVGPQVGKELAENGALALLLVIIGIVIYLALRFEWRF
                     SVSAIIANLHDVIIILGFFAFFQWEFSLSVLAAVLAVLGYSVNESVVVFDRVRETFKK
                     VRGLTTPQVLDHAITSTISRTIITHGSTQLMVLSMLLFGGETLYYFALALTIGICFGI
                     YSSVLVASPLVMWLGVSRGNFVKPKKQIEGANDDGAVV"
     gene            complement(2036..>2730)
                     /gene="secD"
                     /locus_tag="PLD53_11325"
     CDS             complement(2036..>2730)
                     /gene="secD"
                     /locus_tag="PLD53_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRC61553.1"
                     /translation="GGGRVQISGSMSTMEANDTALLLRSGSLAAPMEIIEERTIGPSL
                     GAENIEKGFNSTMWGFAGIAVFMIAYYLLFGLVSVIALASNLLFLVALLSLLQATLTL
                     PGIAAIALALGMAIDANVLINERIREELRNGSTPQHAIHTGYERAFDTILDSNITTLI
                     AGIALLIFGSGPVRGFAVVHCLGILTSLFSAVVVSRALINLIYGRRRKLESLAIGQIW
                     KPGNTTTAAKAK"
BASE COUNT          607 a          751 c          842 g          530 t
ORIGIN      
        1 tcgcgctggc aacgcgatct gaccgattcc acggtgttgc gcaatatggg tgtggccctg
       61 ggttatacct tgctggcgta tgattcgctg ctgcgcggac tgaacaagct cgaagtcaat
      121 cccgactgtc tgcgtgacga tctcgatgcc aactgggagc ttctcgccga accgatccag
      181 accgtgatgc gtcgttacgg cgtcgccaat ccctatgaac gactcaagga actgactcgc
      241 ggcaaccgca tttcccgcga gggcattcag gcctttgtgc aagcgctgga catcccacag
      301 acggcgcggg acgaactgct caggttgacg ccatgggact acaccgggaa ggcggccgaa
      361 ctggcgagga ggatctgatg gtcgatattc cgcataaggt catggtctgc ccgggctgcg
      421 cgcgagaggt gccttctggc gcgccctatt gtccttactg ttgcggagag gacggccggg
      481 agggtgcgct caggcgcggc ggcttcatcg gcgggatctt cggactgatg gcgggcggtc
      541 ttgccgcctc cgtgtggttt gcgttcatcg gtcccgagca tgcgacctgg aatgcgacgc
      601 tcggcatggt atttggcggc gtgattgccg ggctggttct gggactgatt catcaacgga
      661 aaagatagtc ccagataatc cccgatgcat ggtgtgagaa cgaaagacca aagaaacaaa
      721 cccaaggagc tggataggtg tctcaatttg ctgcaaatct aaagaaactg ccgggaattt
      781 cgcacctggc ggcgctcaac ctgctcgatg ccgagggccg tgtgctcgtt gccatcgaga
      841 acaaggccgg tagccagggg tcgctggcgg tctacaacca tctggcgcag acttttggcg
      901 cgattacacc ggacgcggct agaaagggac tcgaactttt tgccgaacat accgaggatg
      961 cccgcatcaa tccgggtaag catcccaata tcgaccggtt gctcggtctg atcgagagca
     1021 aggaaacctt acgcatcaag cacgtttttg cggtttgaac gagaccttgc ttcgggagtc
     1081 agacgaccgc accgtcgtcg ttggcgccct cgatctgctt cttcggtttg acgaaatttc
     1141 cgcgcgacac gcccagccac atgaccagcg ggctggcgac gagtaccgac gagtagatgc
     1201 cgaagcaaat gccgatcgtc agcgccagtg cgaagtagta cagcgtctcg ccgccgaaga
     1261 gcaacatcga cagcaccatc agttgggtgc tgccatgggt gatgatggtg cgcgagatgg
     1321 tactggtgat ggcgtggtca agcacctggg gagtggtcag gccacgcact ttcttaaaag
     1381 tttcgcgaac ccggtcaaag acaacgaccg attcgttgac cgagtagccg agcaccgcga
     1441 gaacagccgc cagcacggaa agtgaaaact cccactggaa gaaggcgaag aagccgagga
     1501 tgatgatgac atcgtgcagg ttggcgatga ttgccgacac cgaaaagcgc cactcgaagc
     1561 gcagagccaa atagatcacg atcccgataa taaccagcag caacgctagc gcaccgtttt
     1621 cggcgagttc cttgccgacc tgcggtccga caaattcgac gcggcgcaag gtggcgccag
     1681 gggcctccgc gtcaagcgca gacattaccg cctcgccgat cttggccgtg ttctgatcgg
     1741 ccttgagcgg caggcgaatc atgacatccc ggctggagcc aaaattctgc acggtagtat
     1801 cgttatagcc cgccttgccg agccctgcgc gaatctgttc cagattggcg gcttgtgcgt
     1861 agttaacctc gatcagcgtg ccgcccgtaa actcgacgga cagatgcagt ccgttgtaga
     1921 acaggaagaa aaccgaaaac aggaaggtga gcagcgagat gacgttgaac accagcgcat
     1981 agcgcatgaa cggaatgtct tttttgatgc ggaaaaattc cattgttgtt cgcccctatt
     2041 tggctttagc cgcagtggtg gtgtttccgg gtttccagat ctggccgatg gctagcgact
     2101 caagcttccg gcggcgcccg tagatcagat tgatcaaggc tcgtgaaacg accactgcgg
     2161 agaacaggga tgtcaggatg cccagacagt gcacaacggc aaagccgcgt accgggccgg
     2221 aaccgaaaat cagcagcgca atgccggcga tcagtgtcgt gatgttcgag tcgagaatcg
     2281 tgtcgaaggc gcgttcgtag ccggtatgga tggcgtgctg cggggtcgag ccattgcgca
     2341 gttcttcgcg aatgcgctcg ttgatcagta cgttggcgtc gatggccatg cccagagcca
     2401 gagcgatggc ggcaatgccc ggcagggtca acgtggcctg tagcagcgag agcaaggcaa
     2461 ccaggaaaag caggttggag gccagggcaa tcaccgaaac cagaccgaac agcaggtagt
     2521 aggcaatcat gaagaccgcg atgccggcga agccccacat cgtcgaattg aaaccctttt
     2581 caatgttttc agcgcccagg ctgggaccga tggtgcgttc ttcgatgatc tccattggtg
     2641 ccgcgagcga accggaacgc aacagcagcg ctgtatcgtt ggcttccatg gtgctcatgc
     2701 tgccggaaat ctgtacgcgt ccgccgccga
//
LOCUS       DAORPM010000292         2681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_13660_length_2681_cov_10.142422, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000292 DAORPM010000000
VERSION     DAORPM010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13660_length_2681_cov_10.142422"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLD53_11330"
     CDS             <1..190
                     /locus_tag="PLD53_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRC61554.1"
                     /translation="NYRKSLRLPLAFLTGLAGYAVGLVLSMLFDLPSGALIVWSLCPL
                     AMLVYAFGPAPTAHHSNA"
     gene            270..2039
                     /gene="ycaO"
                     /locus_tag="PLD53_11335"
     CDS             270..2039
                     /gene="ycaO"
                     /locus_tag="PLD53_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HRC61555.1"
                     /translation="MQAEHFIDGKDASLESSIKRMQEKLALIGFQVVERSWLNPVDDI
                     WSVHVSELDCPLLFTNGKGSSRLAALASALGEFFERLSCNYFWTHYYLGEKYSTHHFT
                     HYPQERWFPLEQDGRWPQELLTPELHDFYNPEKSITADILVEHNSGNVDRGICALPFI
                     RQRDGTSIWFPVNIIGNLYVSNGMSAGNTLAEARTQALSEIVERHIKFRIISEGLCLP
                     EVPGEVITRYPRIVAGIQALRAAGFGVLVKDASLGGKYPVMNVTLLNPDDQGCFSSFG
                     AHPRFEIALERALTELLQGRALDMLDGFPEPGFDLDEIAGSPNLEIHFVDSSGIISWG
                     FLGDTPDSDFCDWNFSATTSEDLAWLINMIQAEGHEIYVADYTHLGVYACRIIVPGMS
                     EIYPVDDLEFENNSFGNEIRDAILHLPELEDAECAALLDTLNNSGLADQRPVAALIGL
                     APDAGSFWEDLRVGELKTLLALAIGNEAAIREGCDWIEHFEQINPDRRRIYRCIGTLL
                     QLSDTGECEPYRKALGILVGKTGLDKAEALLSRKERFFGIQAPGMSLRGCKMHQQLLR
                     AYEKVHSQSIEKAELAELMPDSG"
     gene            2079..>2681
                     /locus_tag="PLD53_11340"
     CDS             2079..>2681
                     /locus_tag="PLD53_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRC61556.1"
                     /translation="MKLETLAVHAGYSPEPTTKAVAVPIYQTTSYSFDNTQHGADLFD
                     LKVVGNIYTRIMNPTQDVLEKRVAAMEGGIGGLALASGQSAITYAIMTIAEAGDNIVS
                     ASTLYGGTYNLFAHTLPQYGINVRFADYRDPATFEKQIDGKTKAIYCESVGNPLGNIT
                     DFEKLAEIAHRNGIPLIVDNTVPSPYLCRPFEHGADIVVHS"
BASE COUNT          599 a          794 c          707 g          581 t
ORIGIN      
        1 caactacagg aagagcctgc gcctgcccct ggccttcctg accggcttgg ctggatacgc
       61 cgttggtctg gtgttgtcga tgctttttga cttgccatcg ggcgccctga ttgtctggtc
      121 actctgcccc ttggcgatgc tggtctacgc gttcggcccc gcaccaacgg cacatcattc
      181 aaacgcatag gcatcgcagg ttcgcataca ctttgctggc gttatgccga cactcatcaa
      241 atccgttcaa ctcctcaatg gacccagcca tgcaagcaga acacttcatt gacggtaaag
      301 acgcctccct ggaatcgtcg atcaagcgca tgcaggagaa attggcgctg attggtttcc
      361 aagttgtgga gcgatcatgg ctcaatccgg tggatgacat ctggtctgtg catgtcagcg
      421 agctcgactg cccactgctg ttcaccaatg gcaaagggtc ctcgagactg gccgcactgg
      481 ccagcgcgct cggcgaattc tttgaacggc tttcctgcaa ctacttctgg acacattatt
      541 acctgggcga aaagtactcc acacaccact tcacgcacta tccacaggaa cgctggttcc
      601 cgctggaaca ggatggcaga tggccacagg aattgctgac gcctgaattg cacgatttct
      661 acaatccgga aaagagcatc acggcagaca tactcgttga acacaattcc gggaatgttg
      721 atcgcggcat ctgtgccctg cccttcatcc gccagcgcga cggaacgagt atctggttcc
      781 cggtcaacat tatcggcaac ctgtatgtaa gcaacggcat gtcggctggc aatacactgg
      841 ccgaggcacg tactcaggcg ctttccgaaa tcgtcgagcg acatatcaaa ttccgcatca
      901 tcagcgaggg cctgtgcctg ccggaagttc cgggcgaggt catcacccgc tatccgcgta
      961 tcgtggctgg cattcaggca cttcgcgcgg ccggcttcgg cgttctggtc aaggacgcct
     1021 cactcggcgg gaagtatccg gtcatgaatg tcacgctgct caatcccgac gatcaaggct
     1081 gcttctcaag ctttggcgct cacccccgtt ttgagattgc ccttgaacgc gcgctcaccg
     1141 aattgctgca aggtcgcgcg ctcgacatgc tggacggttt ccccgaaccg ggttttgatc
     1201 tggacgaaat tgcaggatcg cccaacctcg aaatccattt cgtcgattcg agcggaatca
     1261 tcagttgggg cttcctcgga gatactccgg attctgattt ctgtgattgg aatttcagtg
     1321 ccacgacgtc cgaggatctt gcctggctaa tcaatatgat ccaggcagaa ggccacgaaa
     1381 tctacgtcgc cgactacacg catctgggcg tctatgcctg ccggatcatc gttccgggaa
     1441 tgtccgagat ctatcccgtt gatgatctcg aattcgaaaa caacagtttt ggcaacgaaa
     1501 ttcgcgatgc cattttgcat ctgccggaac tcgaagacgc ggaatgtgcg gcgctgctgg
     1561 acaccttgaa caactccggc ctcgccgacc agcgtccggt cgcagcactg atcggtttgg
     1621 cgcccgatgc gggatcgttc tgggaagacc tgcgtgtcgg cgaactgaag accctgctgg
     1681 ctttggccat cggtaacgaa gcagccatcc gcgaaggttg cgactggata gaacacttcg
     1741 agcagatcaa tcctgatcgt cgccgcatct accgttgcat cggcacgctg ctccagctca
     1801 gtgacacggg tgaatgcgaa ccgtaccgaa aagcacttgg aatccttgtc ggcaaaacgg
     1861 gactcgacaa agccgaagcg ctcctcagcc ggaaggaacg tttcttcggc atccaggcac
     1921 cgggcatgag tctgcgtgga tgcaagatgc accaacagct gctgcgcgcg tacgagaaag
     1981 tacatagcca aagcatcgag aaagcggaac tcgctgaact catgcctgat tcaggctaag
     2041 attgcggttt tacatccaac actgccacgg gagctccgat gaaactcgaa acccttgccg
     2101 tccatgccgg ttatagccct gaacccacaa caaaggcggt ggctgtaccg atctaccaga
     2161 ccacttcata ttctttcgat aacacacagc acggcgccga cctgttcgac ctgaaggtgg
     2221 taggcaatat ttacacacgc atcatgaacc cgactcagga tgtgctggaa aagcgagttg
     2281 ccgcgatgga aggaggcatt ggcggactcg cattggcctc cggccagtcg gccatcactt
     2341 acgcgatcat gaccattgcc gaagccggcg acaatatcgt ttcggcatcg acactgtacg
     2401 gtggcacgta caaccttttt gctcacacgc tgcctcaata tggcatcaat gtacgctttg
     2461 ccgactaccg tgacccggcc acttttgaaa agcaaatcga cggcaagacc aaggcgattt
     2521 actgcgaatc ggtcggcaac ccgcttggca acatcacgga ttttgagaag cttgccgaga
     2581 tcgcgcaccg caacggcatt ccgctgatcg tcgacaacac ggttccatcg ccctatctgt
     2641 gccgcccttt cgagcacggg gccgacatcg tcgtgcactc g
//
LOCUS       DAORPM010000293         2681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_13661_length_2681_cov_9.024752, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000293 DAORPM010000000
VERSION     DAORPM010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13661_length_2681_cov_9.024752"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..978)
                     /gene="ispH"
                     /locus_tag="PLD53_11345"
                     /gene_synonym="lytB"
     CDS             complement(58..978)
                     /gene="ispH"
                     /locus_tag="PLD53_11345"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRC61557.1"
                     /translation="MQITLANPRGFCAGVERAIAIVERALEKFGAPIYVRHEVVHNKF
                     VCDDLRAKGAIFIDDLADVPAGCTVIFSAHGVSQAVREEAAMRGLKIFDATCPLVTKV
                     HVEVGKMRKAGRRIIMIGHKGHPEVEGTMGQSQEGMYLVETVADARQLDIDPASELAY
                     VTQTTLSVDDAENVVAALKERFPKIIGPKRDDICYATQNRQDAVKELAANTDVVFVVG
                     SQTSSNSNRLREVAEHLGVKAHLIDDAEQIDPAWLDGVERIGVTAGASAPEVLVKNVV
                     DKLSSGQNGQVRQLDGVEENVTFALPKELQ"
     gene            complement(987..1442)
                     /locus_tag="PLD53_11350"
     CDS             complement(987..1442)
                     /locus_tag="PLD53_11350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRC61558.1"
                     /translation="MQRPNDLPVQTVRPDSYLTLHYRLSDGEGNEFVSTFDLSPATLQ
                     MGSGQLAAPLENCLVGMPVQQRRVFQLDSSEAFGEHNPKLVERIARSALPADVELKEN
                     SLIEFGAPDGGAGFAGFLRELSDTTALFDFNHPLAGKPIRFEVEVIGIM"
     gene            complement(1451..1990)
                     /gene="lspA"
                     /locus_tag="PLD53_11355"
     CDS             complement(1451..1990)
                     /gene="lspA"
                     /locus_tag="PLD53_11355"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRC61559.1"
                     /translation="MVRGNPVPARNIKPWLWLAGAIIALDQVSKWIVLGVLQVGDSRY
                     VAPFFNWVLTFNPGAAFSFLSDAGGWQRWFFTVLALGVSAWIVGMLSRHSGEFRLSLA
                     LSLILGGALGNVIDRIRFGAVVDFVQWHMAGYYFPAFNVADSAITIGAILLAWDQFAG
                     KPSTDGAVKSAHDVSNDLR"
     gene            complement(1959..>2681)
                     /locus_tag="PLD53_11360"
     CDS             complement(1959..>2681)
                     /locus_tag="PLD53_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRC61560.1"
                     /translation="EIDRYALAMTRQMQAQCEADYGKYEFHRVVQALQTFCSEDLGGF
                     YLDILKDRLYTAAADSAARRSAQSALWHITQAFVKLLAPILSFTAEEVWKTLTRKTES
                     VMLQTWQELPAPADEALLLDKWGKIRLYRAEVTRALEELRVAGLIGSSLQAEVRIHTD
                     GEKYDILASLGDDLRFVLICSKTSLVRSAEEKLECSALSHAKCGRCWHVREDVGQDPE
                     HPEICKRCVSNLHGQGESRACA"
BASE COUNT          511 a          786 c          838 g          546 t
ORIGIN      
        1 ctatgagcca atgtgttggc ctgcaccaca agcggctttg tgagctattg caactggcta
       61 ctgcaactcc ttcggcaagg cgaacgtcac gttttcttca accccgtcga gctggcgaac
      121 ctgaccattc tgcccgctac tcagcttgtc cacgacgttc ttgacgagta cttccggcgc
      181 tgaggcgccg gccgtgacgc cgatgcgctc gacgccgtcg agccaggccg ggtcgatctg
      241 ctcggcatcg tcgataaggt gggcttttac gccgagatgt tcggccacct cgcgcagccg
      301 gttggagttg gagctggttt ggctgccaac gacgaacacg acgtcggtat tggcagccag
      361 ttccttgacg gcgtcctggc ggttctgtgt ggcgtagcag atgtcgtcgc gcttggggcc
      421 gatgatcttc gggaagcgtt ccttcagggc ggcaaccacg ttttcggcgt catcaaccga
      481 caaggtggtt tgcgtgacgt aagccagttc gctggcagga tcgatgtcca gttgcctggc
      541 gtcggcaacg gtttcgacga ggtacattcc ttcctggctc tgccccatcg tcccttcgac
      601 ttcgggatgc cccttgtgcc cgatcatgat gatccggcga cccgctttcc gcattttccc
      661 cacctcgacg tgaaccttgg tgaccagggg gcatgtcgcg tcgaatatct tgaggccgcg
      721 cattgcagct tcctcgcgca ctgcctgcga gacgccgtgg gcgctgaaaa tgacggtaca
      781 gccggccggc acgtcggcga gatcgtcgat gaagatggcg cctttggcgc gcaggtcgtc
      841 gcagacgaac ttgttgtgta cgacttcatg gcggacatag atcggcgcgc cgaatttttc
      901 gagggcgcgc tcgacgatgg caatggcgcg ttcgacgccg gcacagaagc cgcgtgggtt
      961 ggcgagagtg atttgcatgg cggaccttac atgatgccga tgacttctac ttcaaagcgg
     1021 atcggcttgc cggcgagcgg gtggttgaaa tcaaagagcg ccgtggtgtc ggacagttcg
     1081 cgcaggaagc cggcgaaacc ggcgccgcca tcgggcgcgc caaactcgat cagcgagttc
     1141 tctttgagtt cgacgtccgc aggcagggca ctgcgcgcaa tacgttcgac aagtttcgga
     1201 ttgtgttcgc caaaggcttc actggaatcg agctggaata cccggcgctg ttggaccggc
     1261 atcccgacga ggcaattctc cagcggtgca gccagctggc cgctgcccat ctggagcgtt
     1321 gcaggcgaaa ggtcgaaggt gctgacaaac tcattccctt cgccatcgga cagacggtaa
     1381 tggagcgtca ggtagctgtc ggggcgtacc gtttgcacgg gtaagtcgtt gggtcgttgc
     1441 atggagtgag tcaccgtaaa tcgttagaaa catcatgggc ggatttgacc gcgccatccg
     1501 tcgagggttt ccctgcaaat tgatcccagg ccagcaggat ggcgccaatg gtgattgccg
     1561 aatcggccac attgaacgcc ggaaaataat aacccgccat gtgccactgg acgaaatcga
     1621 ccaccgcacc gaagcgtatg cggtcgatca cgttgccgag tgcaccgccg agaatcaggg
     1681 aaagcgccag cgagaggcgg aactcgccgc tatgacggct gagcatgccg acgatccagg
     1741 ccgagacgcc cagcgccagc accgtgaaga accaacgctg ccaaccgccg gcatcggaaa
     1801 ggaaactgaa cgccgcgcct gggttgaagg tcagcaccca attgaaaaac ggtgccacat
     1861 agcgcgaatc tcccacttgc agtacaccga gaacgatcca cttgctgacc tgatctaggg
     1921 cgatgatcgc tccggccagc cacagccatg gcttgatgtt acgcgcaggc acgggattcc
     1981 ccctgaccat gcaggttgct cacacaacgc ttgcagattt ccgggtgttc ggggtcctgg
     2041 ccgacgtctt cgcgcacatg ccagcatcgc ccgcatttgg cgtggctcaa tgcgctgcat
     2101 tcaagttttt cctcggcgct gcgcaccagg ctggttttcg agcagatgag gacaaagcgc
     2161 aggtcgtcgc cgagcgaagc cagaatgtcg tacttctcac cgtcggtatg gatcctgacc
     2221 tcggcttgta gcgacgagcc aatcaggcct gcgacgcgga gttcctcgag ggcacgcgtc
     2281 acctctgcgc ggtagagacg gatcttgccc catttgtcga gcagcagggc ttcgtcagcc
     2341 ggcgcgggca gttcttgcca agtttgcagc atgactgact cggtcttgcg cgtcagtgtt
     2401 ttccagactt cctccgcggt aaaagagaga atcggcgcca gcagtttgac gaaggcttga
     2461 gtgatatgcc acagcgcgct ttgtgccgat cggcgcgcgg cggagtcggc ggcggcggtg
     2521 tacaggcggt ccttgaggat gtcgagatag aagccgccaa ggtcctccga acagaaggtt
     2581 tgcagcgcct gcacgacgcg gtggaattcg tacttgccgt agtcggcctc gcactgcgcc
     2641 tgcatctggc gcgtcatggc cagcgcgtag cggtcgattt c
//
LOCUS       DAORPM010000294         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_14068_length_2629_cov_9.268065, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000294 DAORPM010000000
VERSION     DAORPM010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14068_length_2629_cov_9.268065"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLD53_11365"
     CDS             complement(<1..988)
                     /locus_tag="PLD53_11365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRC61561.1"
                     /translation="MRFTPALFKHAEVLLSELLRSPFPADLVVSRYFRQHRELGHADR
                     GFVAESVYSVLRRKRSLSARCLDDVTSRRLLLASLACVQGMNRRELDAVLSEVEGKWL
                     AQAKALRLEDLPPAIRLDLPDWLYETLKTQFDTQELESLVIGLNQSAPLDLRVNLLKA
                     DRELVLARLNADGLTAEPCRYSPLGIRLLGKPALSRHPLFLDGSFEVQDEGSQLLGFL
                     LQPKRGEMVADFCAGAGGKTLLLGAMMRSQGRLYAFDVAEKRLAKLKPRLARSGLSNV
                     HPVRIESENDIKIKRLAGKLDRVLVDAPCSGLGTLRRNPDLKWRQTPESVVEL"
     gene            complement(988..1959)
                     /locus_tag="PLD53_11370"
     CDS             complement(988..1959)
                     /locus_tag="PLD53_11370"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRC61562.1"
                     /translation="MPFALLIAVAASFGIHAVVLFGPDIDLSYEPESSPLMAELKPMP
                     PPKPKIEPVVKREKHTVAKAKPPPQKVETANSAAPLPGLPEVSPAAVEAPSVEADSVA
                     EPESEPVSVVRSRMPEHGRILYRVDRGDMKFEIGKAEQDWEIVDGRYRLLSVVETTGL
                     VRLFKSYTIEMESRGLMTAEGFHPETFAIRRNGKETKEKASFDWENMKVHVGDQVEQV
                     LHPGAQDLLSFNYQLGFLPLPEAGNSIAIATGRKYGVYQLEVLGDEEIEVPAGVLRTL
                     HLRAPGVNTTELWLAYDYLMLPVKIRHVDRKGDSLVQVAIKIEVSPE"
     gene            complement(1967..2623)
                     /gene="purN"
                     /locus_tag="PLD53_11375"
     CDS             complement(1967..2623)
                     /gene="purN"
                     /locus_tag="PLD53_11375"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRC61563.1"
                     /translation="MKSIVILISGRGSNMESLLGAAATGALPVTVAAVISNRPDAKGL
                     ETAAAHGVSTRVVDHRQFSSREAFDEVLAGVIDSFSPDLVVLAGFMRILSDGFVTHYE
                     GRLINIHPSLLPSFPGLHTHLRALEEGVRVHGCTVHFVTTALDHGPIVVQAAVPVLDG
                     DDEASLAARVLVQEHTIYPLAVRWFAEGRLRLIDGRVEFDAPQASEGALISPLARISP
                     "
BASE COUNT          594 a          801 c          719 g          515 t
ORIGIN      
        1 tcagctcgac cacgctttcc ggcgtttgcc gccatttcag atcgggattg cgacgcagcg
       61 tcccgagtcc ggaacacggc gcgtccacca gtacccgatc aagcttgcca gccagccgct
      121 tgatcttgat gtcattctcg gattcaatgc gtaccggatg cacattcgac agtccagaac
      181 gcgccaaacg cggcttcaac ttggccagac gcttctcagc cacatcaaag gcgtaaagcc
      241 ggccttgaga gcgcatcatg gcccctagca aaagtgtctt gccgccggct ccagcacaga
      301 aatcggcgac catctcgccg cgtttcggct gcaagagaaa gccgagcaac tggcttcctt
      361 catcctgcac ctcgaagctg ccgtcgagaa acaaggggtg gcgcgaaagt gccggcttac
      421 ccagcaagcg aatgccaagc ggcgaatagc ggcagggctc tgccgtcaga ccatcggcat
      481 tcaggcgggc caatacgagt tcgcgatctg ccttgagcag attaacgcgc agatcgagtg
      541 gagcggactg gttgaggccg atgaccagtg actccaattc ctgagtgtcg aactgcgtct
      601 taagcgtctc gtacaaccaa tccggcagat caagtcgaat cgccggtggc aaatcctcca
      661 gcctgagtgc cttggcctgc gccagccact tgccctccac ttcgctcaat accgcatcca
      721 gctctcgccg gttcatgcct tgcacacagg ccagcgacgc gagcaatagt cgccgcgagg
      781 tcacgtcatc aagacagcgc gcagatagcg aacgcttgcg gcgaagcacc gaataaacgc
      841 tttcagcgac aaagccacga tcggcatgac ccagttcgcg atgttggcgg aagtagcggg
      901 aaaccaccag atcggcggga aatggagagc gtaacaactc tgacagcagt acctcggcat
      961 gtttgaaaag ggcaggtgta aagcgcatta ttctggactc acttcaattt tgatggcgac
     1021 ctgcacgagg ctgtcgccct tgcggtcgac gtggcggatt ttaaccggca gcatcagata
     1081 gtcataagct agccagagtt cggtcgtatt cacaccagga gcgcgcaagt gcagtgtgcg
     1141 tagaacacca gccggcacct cgatttcctc atcgcccagc acctcgagtt gatagacacc
     1201 atattttcgg cccgtcgcta ttgcaatcga attgccggcc tcggggagcg gcaagaatcc
     1261 gagctgatag ttgaacgaaa gcagatcctg agcaccggga tgaagaacct gttcaacctg
     1321 atcgccgacg tgtactttca tgttctccca atcgaacgag gccttctctt tggtttcctt
     1381 gccattgcgc cgaatcgcaa acgtttccgg gtgaaaccct tcggccgtca tcaagcctcg
     1441 gctctccatt tcgatggtat acgatttgaa cagcctgacc aggcccgtgg tttcgactac
     1501 ggaaagcagc cggtaacgtc catcgacgat ttcccaatcc tgctctgctt tcccgatctc
     1561 gaatttcatg tcgccgcgat caacccggta gagaattctt ccgtgttccg gcatacgcga
     1621 cctgacgact gacacgggct cagactccgg ttcagcaacg gagtccgctt caaccgatgg
     1681 tgcttcaacc gctgccggcg acacctccgg cagacctggc agcggcgcgg cactgttcgc
     1741 agtttccact ttttgcgggg gaggcttcgc tttggcaacc gtatgttttt cgcgctttac
     1801 tacaggctcg atcttcggct ttgggggtgg cattggcttc agttctgcca tcagtggcga
     1861 cgattcgggc tcgtaagaca ggtcaatatc gggcccgaac agaaccactg catgtatgcc
     1921 gaacgaagcg gcaacggcga tgagcaatgc gaaaggcatg agccgcctac ggtgaaattc
     1981 gagccagcgg cgaaatcagt gccccctcgc tcgcttgcgg tgcgtcaaac tcgactcgcc
     2041 catcaatcag acgcaaacgg ccttcggcga accagcgcac ggccagcgga taaatcgtgt
     2101 gctcctgaac caggacccgt gccgcaaggc tggcctcatc atcgccgtcg agcaccggca
     2161 ctgccgcctg tacgacaatc gggccatgat cgagcgccgt tgtaacgaaa tgaacggtgc
     2221 agccatgcac cctgacgccc tcctcaagag cgcgcaggtg ggtatgcaga cctggaaagg
     2281 atggcagcaa cgaaggatgg atattgatta gccgcccttc gtaatgcgtc acaaagccat
     2341 cgctcagaat ccgcatgaaa ccagccagca cgacaagatc aggtgaaaag gaatcaatca
     2401 cacctgccag gacttcatca aaggcctcgc ggctggaaaa ctgccggtga tcgacaaccc
     2461 gcgtcgaaac accatgcgcg gccgccgttt ccagcccttt tgcgtccggg cggttggaaa
     2521 ttaccgccgc gacggtcacc ggcagcgcac ccgtagcggc cgcgccaagc aaggattcca
     2581 tgttgctgcc ccggccggag ataagaatga cgatggattt catctgatt
//
LOCUS       DAORPM010000295         2626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_14097_length_2626_cov_9.644107, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000295 DAORPM010000000
VERSION     DAORPM010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14097_length_2626_cov_9.644107"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /gene="rnhB"
                     /locus_tag="PLD53_11380"
     CDS             <1..212
                     /gene="rnhB"
                     /locus_tag="PLD53_11380"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287507.1"
                     /note="RNH2; RNase HII; binds manganese; endonuclease
                     which specifically degrades the RNA of RNA-DNA hybrids;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRC61564.1"
                     /translation="IASIAAASILAKTVRDAGMRVLHAQYPQYGFDRHMGYPTAAHFK
                     ALEEHGASPVHRRSFGPVAKQLSLL"
     gene            209..982
                     /locus_tag="PLD53_11385"
     CDS             209..982
                     /locus_tag="PLD53_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRC61565.1"
                     /translation="MKLIQSRDNPFFKGLKRLAESGRERRKTGHTLLDGVHLVESYEA
                     VHGQVETLIVAESALASGEIAAYVAGRDVVVLADSLLRDLGLVDTPSGLLAVATMPSG
                     ISAVNLEKEAILLDGVQDPGNVGTLLRTAAAAGIKQALLSPGCASAWSPKVLRAGQGA
                     HFVLAIHEDVDLAEFMAAYCGTTAVTCLDGATSLYEARWHGPLAWVFGAEGQGVRPEL
                     MAAARLKIKIPMPGAVESLNVAAAAAICLFEALRRKLVP"
     gene            complement(998..1816)
                     /locus_tag="PLD53_11390"
     CDS             complement(998..1816)
                     /locus_tag="PLD53_11390"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRC61566.1"
                     /translation="MPTPIKRTVFFVSDGTGLTAEALGHSLMTQFEDVEFKQIRIPFL
                     DNIAKAQEAVARINAQGEADGMRSIVFTTLVNQALSSIVHQADAFCLSYFETFLAPLE
                     AELGIKSSHTVGRSHGTADSSDYKKRIESINYTLAHDDGITDRDLAEADVILVAVSRC
                     GKTPTSLYLAMQFGLKAANFPLIPEDFDRGRLPGTLEKHRSKLFGLTIQPERLHHIRE
                     ERMAGSKYASLANCRYEIAEAEKMMRREGIRWLDSSTKSIEEISTTVLQELKLR"
     gene            complement(1826..>2626)
                     /locus_tag="PLD53_11395"
     CDS             complement(1826..>2626)
                     /locus_tag="PLD53_11395"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRC61567.1"
                     /translation="TKYGNPDTGRRNLEVLLAATLEASLTDHENRVEPAEQFHGVMDQ
                     LSSLAFNAYRGLVYETPGFTTYFRQSTVVSEIATLNIGSRPASRKPSERIEDLRAIPW
                     VFSWAQCRLMLPGWYGFGSAVDGYLKANPEGLATLHRMVHAWPFFKSLLSNMDMVLAK
                     TDLAIASRYAELVSDPALRDSIFSRITAEWALTRQHLLAILEQDDFLADNPMLKRSLQ
                     LRSPYMDPLNHLQVELLKRHRAGETDERVARGIHLSINGVASGLRNSG"
BASE COUNT          494 a          716 c          839 g          577 t
ORIGIN      
        1 agattgcctc gattgccgca gcctcaattc tcgccaagac cgttcgcgat gccggtatgc
       61 gggtcttgca tgcgcagtat ccgcaatacg gttttgatcg ccatatgggt taccccacag
      121 cggcgcattt caaggcgctg gaagaacacg gggcttcgcc ggtgcatcgc cgtagtttcg
      181 ggccggttgc caaacagctt tcgctgttat gaagctgatt cagtcacgcg acaacccgtt
      241 tttcaaaggc ttgaaacggc tggccgagtc cggtcgcgag cgccggaaga cggggcatac
      301 gctgcttgat ggcgtgcatc tggtcgagtc ttatgaagcc gtgcatggtc aggtcgaaac
      361 actgattgtc gccgaatcgg ccctggcttc cggcgagatc gccgcttacg tcgcgggccg
      421 tgacgtggtg gtgctggccg acagtttgct gcgcgatctc ggtctggtcg atacaccgag
      481 cggtttgttg gcagtggcaa cgatgccgag cgggatttct gcggtcaacc tggaaaaaga
      541 ggcaattttg ctggatgggg tgcaggatcc gggcaatgtt ggcacgctgc tacgcactgc
      601 cgctgcggcc ggtatcaagc aggcgctatt gtcgccgggt tgtgcttctg cctggtcgcc
      661 gaaagttttg cgggccgggc agggggcaca ttttgtgctg gctatccacg aggatgtcga
      721 tctggccgaa ttcatggccg cttattgcgg tacgacggcg gtcacttgtc tggatggcgc
      781 aacttcgctt tacgaggcgc ggtggcatgg cccgctggcc tgggtgtttg gtgccgaggg
      841 gcagggcgtt cgtcccgaac tgatggctgc agcccgactc aaaatcaaaa ttccgatgcc
      901 gggtgcggtc gaatcgctca atgttgccgc cgccgctgcc atttgcctgt ttgaagcact
      961 gcgccgaaaa ctggtgccat agaaccaacg gctggttcta acgtagcttc aattcctgca
     1021 ggacggtggt tgagatttcc tcgatcgact tggtcgacga atccagccag cgaatgcctt
     1081 cgcggcgcat cattttttcc gcttcggcga tttcatagcg acagttggcc aacgaggcgt
     1141 atttgctgcc ggccatgcgc tcttcacgaa tatggtgcag tcgttcgggc tggatggtca
     1201 ggccgaacag tttgctacga tgcttttcca gcgttcccgg cagtcgaccg cgatcgaagt
     1261 cttccggaat cagcggaaag ttcgccgcct tgagaccaaa ttgcatggcg agatagagcg
     1321 aagtcggcgt cttgccgcag cgcgagacgg caaccagaat aacgtcagcc tccgcgagat
     1381 cgcggtcggt aatcccgtcg tcgtgggcca gcgtgtagtt gatcgactcg atccgcttct
     1441 tgtaatccga actgtcggcg gtcccgtgcg agcggccgac ggtgtggctg gatttgatgc
     1501 ccagctctgc ttcaagcgga gccagaaaag tttcaaagta ggacaggcag aaggcatcag
     1561 cctgatggac gatgctcgat aacgcctgat tgaccaaggt cgtgaagacg atggagcgca
     1621 tgccgtcagc ctcgccttgg gcattgatgc gggccaccgc ctcctgtgcc ttggcgatgt
     1681 tgtcgaggaa gggaatgcgt atctgcttga attccacatc ctcgaactgg gtcatcagac
     1741 tgtggccgag cgcttctgcg gtcaggccgg tgccgtcgga aacgaaaaat acggtgcgtt
     1801 tgattggtgt aggcattcgc caattttacc cgctgttgcg caatcccgaa gcgacgccat
     1861 tgatcgacag atgaatgccg cgcgccacac gctcgtcggt ttcgccggcc cggtggcgct
     1921 tcaacagttc gacctgcagg tggttgagcg ggtccatgta cggcgagcgc agttgcagcg
     1981 aacgcttgag catgggattg tcggcgagga aatcgtcttg ttccaaaatc gccagcaggt
     2041 gctggcgggt cagtgcccat tccgccgtga tgcggctaaa gatgctgtcg cgcaaggcgg
     2101 gatcgctgac cagctcggca tagcgcgagg cgatcgcgag atcggttttg gccagcacca
     2161 tgtccatgtt cgagagcagg cttttgaaaa atggccaagc gtggaccatg cgatgcagcg
     2221 tcgccaaccc ttcaggattt gcttttaaat agccgtcgac cgcagaaccg aagccatacc
     2281 agccgggtag catcaggcgg cactgcgccc aggagaagac ccagggaatg gcgcgcagat
     2341 cctcgatccg ttccgatggt ttgcgcgagg ccgggcggct gccgatattc agggtggcaa
     2401 tttcggaaac gaccgtcgat tgccggaaat acgtcgtgaa gcccggcgtt tcgtagacca
     2461 gaccacggta ggcgttgaag gccagcgacg atagctgatc catgactccg tgaaattgtt
     2521 cggccggttc gacgcggttt tcgtgatcgg tcaggctggc ttccagcgtc gcggcgagca
     2581 gcacttccag gttgcggcga ccggtatccg ggttgccgta cttggt
//
LOCUS       DAORPM010000296         2568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_14626_length_2568_cov_8.055312, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000296 DAORPM010000000
VERSION     DAORPM010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14626_length_2568_cov_8.055312"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLD53_11400"
     CDS             <1..786
                     /locus_tag="PLD53_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRC61568.1"
                     /translation="SLAFWLWTIALALTLPLALPHLRTQWPLLKRGWPAIVILGLLGV
                     GGYNTLAYVALQYTAATNAVLLNSFIPIATITLSWAFLKKHLRGIEWLGVMMSLIGVI
                     TIVARGKLSTLAGLAPNIGDLWMLGAVFVWAFYTIGLQWRPAGVDPMLLLAALISVGL
                     VALTPAYAWEIAQGRLINVTPSSLAVILYTGIFPGFLGYIFYNRAVGEVGASKASLFI
                     HLMPVFGTLLSAVFLAEVPHGYHFVGIALIFVGIYLTTAIKFR"
     gene            887..>2568
                     /locus_tag="PLD53_11405"
     CDS             887..>2568
                     /locus_tag="PLD53_11405"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRC61569.1"
                     /translation="MRFVDPDRYAALKATGKLPSPKGVALSIIRLLQRDDFRIPELVQ
                     LVQSDPAIAGRILYFANAAAFGRSRPIVSLQRAIVALGSFRVRDLVIGLSVMHSHKSG
                     LCTAFDYEVFWGHSLATGIACQELAHFTQIASEEIFTIGLLARVGELALASLFPDEYS
                     DVLLAAREQRLAMAPLEQERFGMDHHELAATLLGEWGLPEVLITAAFHHENPDAAGFA
                     DGSRVQTLTHSLSFASALAHLCMVDEESRWSLLPSLLARAARLGIGGDALNDLVDRMV
                     ERWREWGAKLNVRTQDIPPFSEILAATPPMRRMGTVPDSENRRALTPCLHVQLIGIPL
                     EDLKPLMQQIEDLGHQPVLVDDLADGLAQVLRRPAQIIIADMSMPGLKATDFCRNLRQ
                     TPTGKECYALLLAPPENEGRILEFIDAGADDVLVKPLNMQTLRVHINTAARMLILKAE
                     IQRERLGIMRSTDEFAVAQKRLLEDALTDTLTQLPNRRKGLDFLASEWVFAQSSSAPL
                     ACLMLDIDHFKKINDNYGHAAGDAVLRQLADVLRNTSRAEDMIFRYGGEEFAA"
BASE COUNT          442 a          840 c          740 g          546 t
ORIGIN      
        1 tcactggcct tctggctttg gaccatcgcc ctggcgctca ccctgccgct ggcgctgcct
       61 cacctgcgca cgcaatggcc gctgctcaag cgcggctggc ccgccatcgt cattctcggg
      121 ctgctcggcg tgggcggcta caacacgctg gcctacgtcg cgctgcagta cacggctgcc
      181 acgaatgccg tcctgctcaa ctcgttcatc ccgattgcca ccatcacgct ctcctgggct
      241 ttcctgaaga aacacctgcg cggtatcgag tggctcgggg taatgatgtc gctgattggc
      301 gtgatcacca tcgtcgcgcg cggcaaactc tccactctcg ccgggctggc acccaacatc
      361 ggcgacctgt ggatgctcgg cgcggtattc gtctgggcct tttacaccat cggcctgcaa
      421 tggcggccgg ccggcgttga cccgatgctc ctgcttgccg ccctgatcag cgtcggcctg
      481 gtggcgctga ctcccgccta cgcctgggaa atcgcccagg ggcgtctgat taacgtcacg
      541 ccctcatcgc tcgctgtcat cctctacacc ggcatctttc ccggctttct cggctacatc
      601 ttctacaatc gtgccgtcgg cgaagtcggc gccagcaagg ccagtctgtt cattcatctg
      661 atgccggtgt tcggcaccct gctttcggcg gtcttcctcg ccgaagtgcc gcacggctac
      721 cactttgtcg gcatcgccct gatctttgtc ggtatctatc tgaccaccgc gatcaaattc
      781 cgctgaagcg tggcatgacc tgtgcggtgt atttttcgcg acaggcggga tcaccggcga
      841 tgcgatcttg ggtttatgat gggccactga ccggagattg tcccccatgc gttttgtcga
      901 tcctgatcgt tacgcggcgc tgaaggcgac gggcaaactg ccctcgccca agggcgttgc
      961 gctgtcgatc atccgactgc tgcagcgcga cgatttccgg attcccgaac tggtgcaact
     1021 ggtccagtcc gacccggcga tcgccggacg catcctgtat ttcgccaatg ccgccgcctt
     1081 tggccgctcg cgcccgatcg tttcgctgca gcgcgcaatc gtagcgctgg gatcgtttcg
     1141 cgtacgcgat ctcgttatcg gcctctccgt catgcacagt cacaagagcg gactgtgcac
     1201 ggccttcgat tacgaagtct tctggggaca ctcgctggcc accggcatcg catgtcagga
     1261 actcgcccat ttcacccaga ttgccagcga agaaatattc accatcggtc tgctggccag
     1321 agtcggtgaa ctggctttgg cttcgctgtt ccccgacgag tactccgatg tcctgctggc
     1381 cgcccgcgag caacgtctgg caatggcgcc gctggagcag gaacgctttg gcatggatca
     1441 ccacgagctc gccgctactc tgctcggcga gtggggactg cccgaggttc ttatcacggc
     1501 ggccttccac cacgaaaacc ccgatgcggc cggctttgcc gacggttcca gggtacagac
     1561 gctgacgcat tcgctgagtt ttgccagcgc gttggcgcat ctgtgcatgg tcgatgagga
     1621 atcgcgctgg agcctgctac cgtcacttct ggcgcgcgcg gcgcgtcttg gcattggcgg
     1681 cgatgcgctg aacgatctgg tcgaccgcat ggtcgagcgc tggcgcgaat ggggtgccaa
     1741 actgaacgta cgcacgcagg acatccctcc cttctcggaa atactcgccg ccactccgcc
     1801 gatgcgtcgg atgggtacgg ttcccgacag cgaaaacagg cgcgcgctca cgccatgcct
     1861 gcacgttcaa ttgatcggta tcccgctgga agacctcaag ccgctgatgc agcagatcga
     1921 ggatctcgga caccaacccg tactcgttga tgatctggct gatggactgg cgcaggtctt
     1981 gcgccgtccg gctcagatca tcatcgccga tatgtccatg cccggcttga aggcgactga
     2041 cttctgccgc aacctccgcc agacgccaac aggcaaggaa tgctatgccc tgcttctggc
     2101 gccgccggaa aacgagggcc gcatccttga attcattgat gccggcgccg acgatgtgct
     2161 ggtcaagccg ctgaacatgc agacccttcg cgttcacatc aacacggcgg cccgaatgct
     2221 gatcctgaaa gctgaaatcc agcgcgaacg cctcggtatc atgcgttcga ccgacgagtt
     2281 tgcagtggct cagaagagac tcctggagga tgcgctgacc gacacgctga cgcagcttcc
     2341 caaccgtcga aaaggtctcg actttctcgc atcggagtgg gtttttgccc agtcaagcag
     2401 tgcgccgctg gcctgcctga tgcttgacat tgaccacttc aaaaagatca acgacaacta
     2461 cggtcatgcg gccggcgacg cggtcttgcg gcaactggcc gacgtcctga gaaatacttc
     2521 gcgcgccgag gacatgatct tccgctatgg cggcgaggaa tttgccgc
//
LOCUS       DAORPM010000297         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_18226_length_2260_cov_11.369615, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000297 DAORPM010000000
VERSION     DAORPM010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18226_length_2260_cov_11.369615"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLD53_11410"
     CDS             complement(<1..193)
                     /locus_tag="PLD53_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HRC61570.1"
                     /translation="MDLPNDWIAMLALVFVFGAKHGLDADHLATIDGLTRYNLRQAPA
                     RARWCGCLFSLGHGAVVMTI"
     gene            535..1716
                     /locus_tag="PLD53_11415"
     CDS             535..1716
                     /locus_tag="PLD53_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HRC61571.1"
                     /translation="MGEMNEIFDLGRKALHDVEKRLDMPRREFLQFCSTLAVAMGLPS
                     GAEAAIANAIEASKRPSVIWLHFQECTGCSESLLRAEHPTLEKLILDVISLDYHETLM
                     VAAGHQAEAARKDAMKANKGQYVLVVEGAIPVKDNGIYCKIGGQTAIDLVKECAADAA
                     AIIAIGSCASWGGMPSTSPNPSGSSSLGEVLGKPVVTIPGCPPNPYNFLATVVHFLTF
                     GKLPDVDQLGRPKFAYSRLIHENCERRAHFDAGRFAIEFGDAGHRKGYCLYKLGCKGP
                     ETYANCPTLGFGDAGENNWPVGCGHPCIGCTEKGVGFTKPIHQVAKMLNVAPPLQYAR
                     IVEEQGKGASFASAAALAALAGAAAGGAAMLTRNLGLSHAAEEAERRKGNSKPQEKTE
                     V"
     gene            1719..>2260
                     /gene="hybA"
                     /locus_tag="PLD53_11420"
     CDS             1719..>2260
                     /gene="hybA"
                     /locus_tag="PLD53_11420"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HRC61572.1"
                     /translation="MNDRRDFLKGVLASGAAISTVAIAPDVCARETRTRPPEGLGLLY
                     DATLCIGCKACVAACKEANDNPPEFSTEDHLWDTPLDTSGYTFNLIKMYRNGTMETKD
                     AEVNGYAFMKTSCMHCADPSCVSACPVSAMMKDPVTGVVAYDPEACVGCRYCVVACPF
                     GIPKYQYDSPTGKIGKCELCR"
BASE COUNT          486 a          623 c          679 g          472 t
ORIGIN      
        1 cgatagtcat tacgacggcg ccatggccca gcgagaacaa gcatccgcac cagcgcgcgc
       61 gcgccggtgc ttggcgcagg ttgtaccggg tcagtccatc gatggtggcc aggtggtcgg
      121 catcgaggcc gtgtttggcg ccaaaaacaa acaccagggc aagcatggca atccagtcgt
      181 tcggcaaatc catgggcgac acctcaggtg tgtttgacag catgttgcag gttgccggaa
      241 aggcagagta ctcgagtatc cgggggcatc atacaagatt cgtgcctgta cgccatcccg
      301 ggcgaaccgc aagtgaatca aggccttggc aagtctaaat atcttcgggt tgcagccgta
      361 ctggttagcg gtgtggttac ctgctaatcg atggcggatc gcgatgttgc gccgcaacat
      421 acgctaagcc tttgaattgg cagactggct tcacagtatg tcagtgtggc acgcgacttg
      481 ctgacgtagc tgacataggt ccggaagtga ccagatacca ggaggaaaga agacatgggc
      541 gagatgaatg aaatctttga tctgggtcgc aaggccttgc atgatgtcga gaaacgcctc
      601 gacatgccgc ggcgagagtt tcttcagttc tgctcgacac tggcagtcgc gatgggtttg
      661 ccgagtggcg ccgaagcggc gatcgccaac gcgatagaag cttcaaaacg gccctcggtc
      721 atctggctgc actttcagga atgcacgggt tgctccgagt cactccttcg tgccgagcac
      781 ccgacgcttg aaaagctgat cctggatgtc atatccctcg attaccacga aaccctgatg
      841 gtcgccgctg gtcatcaggc ggaggcagcc cgcaaagacg ccatgaaagc caacaagggc
      901 cagtatgtgc tggttgttga aggcgcaatt ccagtaaagg acaatggtat ctattgcaaa
      961 atcggtgggc aaacggcgat tgatctggtc aaggaatgcg cggcggacgc ggcggcgatc
     1021 atcgccatcg gctcctgcgc ctcctggggc ggtatgcctt cgaccagccc aaatccctcg
     1081 ggctccagca gcctgggtga agtccttggc aagccggttg tgaccattcc cggttgcccg
     1141 cccaacccgt acaacttcct cgccacggtc gtccattttc tgacttttgg caagctgccg
     1201 gatgtggacc agctggggcg tccgaaattc gcctattcgc gcctgattca cgaaaactgc
     1261 gaacgccgtg cgcactttga cgccggtcgc tttgcgattg aattcggcga tgccgggcat
     1321 cgcaaaggct attgtcttta caagcttggc tgtaaagggc cggaaaccta cgccaattgt
     1381 ccgacactgg gatttggtga tgcgggtgag aacaattggc cggtgggctg tggtcatcca
     1441 tgcatcggtt gtactgaaaa gggggtgggc ttcaccaagc cgatccatca ggttgcgaag
     1501 atgctcaacg tcgcaccacc cctgcagtac gcgcgaatcg ttgaagagca gggtaaagga
     1561 gcttcctttg cttcggcggc tgcactcgcc gccttggccg gtgccgctgc tgggggcgca
     1621 gcaatgctga cccgaaacct tggcttgtcg cacgcggcgg aagaagccga gcgccgcaaa
     1681 ggcaacagca agcctcaaga gaagacggag gtatagatat gaacgaccgt cgtgatttcc
     1741 ttaagggggt tctcgccagc ggtgccgcaa taagcaccgt agcgattgct cccgacgtgt
     1801 gtgcgagaga aacccggact cgacctcccg aagggcttgg gctgctttac gacgccacct
     1861 tgtgtatcgg ctgcaaggcg tgtgttgccg cctgcaaaga ggcaaatgat aatccgcccg
     1921 agttttccac tgaagatcat ctgtgggata cgccactcga tacgtccggc tataccttca
     1981 atctgatcaa gatgtaccgc aatggcacga tggaaacgaa ggacgccgaa gtcaatggct
     2041 acgccttcat gaagacatcc tgcatgcatt gcgccgatcc ctcctgcgtg tcggcatgcc
     2101 cggtttcagc catgatgaag gatccggtta ccggtgttgt tgcttatgac cctgaggcct
     2161 gcgtcggctg ccgctattgc gtggttgcct gcccgttcgg cattccgaaa tatcaatacg
     2221 actcgccgac cgggaagatc ggcaagtgcg agttgtgccg
//
LOCUS       DAORPM010000298         2146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_19913_length_2146_cov_7.437590, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000298 DAORPM010000000
VERSION     DAORPM010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19913_length_2146_cov_7.437590"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLD53_11425"
     CDS             complement(<1..639)
                     /locus_tag="PLD53_11425"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRC61573.1"
                     /translation="MGLHIGDEAPDFSAPTTEGTIRFHEWIGDSWVILFSHPKDFTPV
                     CTTELGYLARLKPEFDKRNTKVIGLSIDSVDDHRRWAGDIAETQGTALNYPLIGDTDL
                     VVAKLYDMIHPNASGNAASRTAVDNATVRSVFLIGPDKKIKLTMTYPMSAGRNFDEVL
                     RALDAVQLNAKHTVATPVNWRPGEDVIIPTSVSEEAAKAKYPGGWKTLKPYLR"
     gene            complement(787..1830)
                     /locus_tag="PLD53_11430"
     CDS             complement(787..1830)
                     /locus_tag="PLD53_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRC61574.1"
                     /translation="MIFEQIATGGCQSYLIGCNESSGAALIDPEISQIDHYIALAARD
                     GLRIRYVIDTHTHADHFSATRQLARQLGVPVVMHRASSAPFVDMRIGDGEMIIVGKLR
                     LHAIHTPGHTRDSMCLRVEDRLFTGDTLLIGATGRTDLPTGDPEALFDSLFHRILRLD
                     ADVKVFPAHDYKGRRNSTIGQELATNPRLQTRERAAFAEMMRNLDLSMPTHITEALRT
                     NMSGGKSVAQLLAEAAARVPFMSLEELRARIQAAEDDLIVLDVRERDAYESGHIPGAR
                     VLPRGQLELRVNQELPDPTRRVLTCCELGYVSTLAASTLREMGFMHTVALDGGMKAWR
                     EAGYPVKVGREPH"
     gene            complement(1966..>2146)
                     /locus_tag="PLD53_11435"
     CDS             complement(1966..>2146)
                     /locus_tag="PLD53_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61575.1"
                     /translation="PVIEIDHDDAPFRSGGKTDILSRQRIDDADNTAKSFYERFQGIP
                     VRDDPACTTGGRAKI"
BASE COUNT          397 a          621 c          668 g          460 t
ORIGIN      
        1 gcgcagatag ggcttcagtg ttttccagcc gcccgggtac ttggccttgg ccgcttcctc
       61 ggagactgag gtggggatga tcacgtcctc tcccggacgc cagttaaccg gcgtagcgac
      121 cgtatgcttc gcattgagct ggacggcgtc gagcgcgcgc agtacctcgt cgaagttgcg
      181 tccggcgctc attgggtagg tcatcgtgag tttgattttc ttgtcgggcc cgatcaggaa
      241 taccgagcgc accgtggcgt tgtccaccgc cgtgcggctg gcggcattgc cgctggcgtt
      301 gggatggatc atgtcataaa gcttggcaac gacaagatcg gtatcgccga tcagcgggta
      361 attcagcgcg gttccctgcg tctcggcgat atcgccggcc cagcggcggt ggtcgtcgac
      421 gctgtcgatc gacaggccga tgactttggt attgcgcttg tcgaattccg gcttcaggcg
      481 ggcgaggtaa cccaattctg tagtgcaaac gggagtgaag tccttcgggt gcgagaacag
      541 aatgacccaa ctgtcgccga tccattcatg gaagcgaatc gtgccttcgg tcgtcggcgc
      601 gctgaaatcc ggcgcttcat cgccaatgtg tagtcccatg gttttctcct tgttgcgctt
      661 tgtgagggaa agtccggtga caagcgaagc gcgcgcttat cttgaccgta cctgcaggcc
      721 gctggtcggc gtaccgttgg actgcatact ttgacggccg ttccacctct tggagacgaa
      781 ttgcggttag tggggttcgc gccctacttt gaccggatag cctgcctcgc gccaggcttt
      841 catcccgccg tccagcgcca ctgtgtgcat gaatcccatc tcccgcaatg tggacgcagc
      901 gagcgttgac acgtagccca gttcgcaaca ggtgaggacg cgccgggttg ggtccgggag
      961 ttcctgattg acgcgcagtt ccaattggcc gcggggcagc acccgtgccc cgggtatatg
     1021 gccgctttcg taggcgtcgc gctcacgcac gtcgagcacg atcaggtcat cttccgccgc
     1081 ttggatgcgc gccctgagtt cttcgagcga catgaacggc acgcgagcag cggcttcggc
     1141 gagcaactgc gcgacggatt ttcccccact catgttggtg cgcagcgcct cggtaatatg
     1201 cgtcggcatg gagagatcga gattgcgcat catctcggcg aaggcggcgc gttcgcgcgt
     1261 ctgcaggcgt ggattggtgg cgagttcctg ccctatcgtc gaatttcgcc gacccttgta
     1321 atcgtgcgcc ggaaatacct tgacgtccgc gtccagccgc agaatgcgat ggaacaggct
     1381 gtcaaaaagc gcttccgggt cgccggtcgg cagatcggtg cggccggtcg caccgatcaa
     1441 cagagtatcg cccgtaaaca aacggtcttc gacccgaagg cacatcgaat cgcgcgtgtg
     1501 gccgggtgta tggatcgcgt gcaagcgcag cttgccgacg atgatcattt cgccatcgcc
     1561 gatccgcata tcgacaaacg gtgcggaact cgcccggtgc atgaccacgg gcaccccaag
     1621 ttgccgtgcg agttgccggg tcgcagagaa atgatcggcg tgggtgtggg tgtcgatcac
     1681 gtagcggata cgaaggccgt cccgggcagc aagcgcaatg tagtggtcga tctggctgat
     1741 ttcggggtcg atcagcgccg ccccgctgga ttcgttgcag ccgatcaggt aggactggca
     1801 gccaccggtt gcgatttgtt cgaaaatcat ctcgttctcc cgggaaacaa agcggcatgg
     1861 cgccatgaga gactgaaaat ggcccttgca tacgcccaag gagacgcaat gatcgcacaa
     1921 tcacgatccg cgcgcagcca acatctacat gcctgtggaa ccacgtcaga tctttgcacg
     1981 tccaccggtg gtgcaagccg gatcgtcgcg gacgggaatg ccttgaaatc gctcatagaa
     2041 ggatttcgcc gtattatcgg catcatcaat tcgctgcctg ctcaaaatgt ctgtcttgcc
     2101 gcctgaccgg aacggagcgt catcatgatc gatttctatt actgga
//
LOCUS       DAORPM010000299         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_19989_length_2142_cov_10.784859, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000299 DAORPM010000000
VERSION     DAORPM010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19989_length_2142_cov_10.784859"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..562
                     /locus_tag="PLD53_11440"
     CDS             185..562
                     /locus_tag="PLD53_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRC61576.1"
                     /translation="MTNKEKALEWIASSYKDLMALPSDVRRRFGYALSLAQMGDQDDA
                     AKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKYAEAVFVLHCFQKKSKRGIATPKSDMD
                     IIRARLKVADALAQELRNAKTNH"
     gene            543..878
                     /locus_tag="PLD53_11445"
     CDS             543..878
                     /locus_tag="PLD53_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRC61577.1"
                     /translation="MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRKAM
                     RALGLTQQEAAKRMGIPQPKVSGMMRGDFTNLSERKLMDCLNRLGYDIEIKVRPAAEP
                     VGHLTLAVA"
     gene            893..1252
                     /locus_tag="PLD53_11450"
     CDS             893..1252
                     /locus_tag="PLD53_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRC61578.1"
                     /translation="MVARITDEEWDKLSPDNFETHSLLRAVDAVDELRADLNDGEYAT
                     PPQLRTDLLKLHQLAMAVINEGSRSQVASLFELASDLDEQVSHMMTNLEEVQATLSQL
                     TALYPDSLCDGGLDGDE"
     gene            1469..2089
                     /locus_tag="PLD53_11455"
                     /pseudo
     CDS             1469..2089
                     /locus_tag="PLD53_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
BASE COUNT          484 a          638 c          650 g          370 t
ORIGIN      
        1 aaacgccgga gacaaagttt ccggccatcc gacctatcag acacggttcc ccgtgcagct
       61 ttcgccctgt cgagaccatc gtttgcgccg cgttgccgtc cgggtgggaa acacttgttt
      121 tgcgacatca acgctactgg tatcgcggtt ttttgtttta cctgaacagg tataaactgc
      181 cgccatgacc aataaagaaa aagcgctcga atggatcgcg agcagctaca aggatctgat
      241 ggcgttgccg tctgacgtgc gcaggcgctt cggttacgca ctgtcgttgg cgcagatggg
      301 cgatcaggac gacgcggcaa aggtgctcaa gggcttcggc ggtgccggcg tgctggaggt
      361 cgtcgaggac gatgccggcg gcacgtaccg ggccgtctac acggtgaagt acgcggaagc
      421 ggtgttcgtc ctgcactgct tccagaagaa gagcaagagg gggatcgcca caccaaagtc
      481 agatatggac atcattcgcg ctcggctgaa agtggccgac gcattggcac aggagctacg
      541 aaatgcaaaa acgaatcatt gaaggcgtcg aggttcagcg cagctcgggc aacgtctttg
      601 ccgaccttgg actgcccgat gccgaaaaac tcaagatcaa gaccggcttg gtggtcgaaa
      661 tccggaaggc aatgcgcgct cttgggctga ctcaacaaga ggcggcgaaa cgcatgggca
      721 tcccgcaacc gaaggtgtcg ggcatgatgc gtggtgactt cacgaacctg tccgaacgca
      781 agctgatgga ctgcttgaac cgcctcggct acgacatcga gatcaaagtg cggccggccg
      841 ccgagccggt cggacacctg acgctcgcgg tcgcttgatc aggtgccagc cgatggtagc
      901 ccgcattacc gatgaagagt gggacaaact gtccccggat aatttcgaga cgcattcgct
      961 gctgcgtgcc gtcgatgccg tggacgaact gcgcgccgat ctcaacgatg gcgaatacgc
     1021 caccccgccg cagcttcgca ccgacctgct gaagctgcac caactggcaa tggccgtcat
     1081 caacgaggga tcgcgcagcc aggtcgcttc tctattcgag ctggccagcg atctggatga
     1141 gcaggtttcc cacatgatga ccaatctgga agaggtacag gcgacgctat cgcaattaac
     1201 ggcgctgtac ccggacagct tgtgcgatgg aggcctggac ggcgacgaat gatggctggc
     1261 cgctcctact ctggcccatc aagcgtcagg tcgccacggc ggatcgcatc gccaaccaca
     1321 agagggacgc aatccgcagt aacgcgcctc cctgaaacag cgcctgccac gcaagtggat
     1381 gagcgaataa aatctggctt actccaaggt ggcggctggt gacacaactg cttgttctac
     1441 ccctacaagc aggcggcggc tgccggcgcg caagggcgag aaagggacgc caatccagta
     1501 ttggaaattc agcgaggaac acgacgaggc cggcaagcct gttctcgacg cccagggaaa
     1561 gccggtgaaa gacgatgttc gtctggagcg accccgggtg ttttttgcca ccgtgttcaa
     1621 cgccgagcag atcgacggcc tgccaccgct gcagccgcgc aaggagcagg aatggaatgt
     1681 cctcgaacgg gttgaacaca tcctgcaggc ctctggtgcg ctgatccagc acggcccgca
     1741 aaaccgggcc ttctaccggc cggggtccga cagcatccat ctccccgaaa agggtcagtt
     1801 tcccagcgcc gacacttact atgcgacagc actgcacgaa ctgggacact ggaccggcca
     1861 cccctcacgc ctggctcgcg acctggcaca ccctttcggc agcgaggcct atgccaagga
     1921 agagctgcgg gcggaaattg ccagcatgat catgggcgat gaactcggaa tcggtcacga
     1981 tcccggccag cacgcggcct acgtgggaac gtggatcaag gtcctgaaag acgacccact
     2041 cgaagtcttc cgcgctgccg ccgacgccga aaagatccac gcctatgtgt agcgacgcaa
     2101 aatgatgtcg gcccagacgc acaatcaagg gggcgttctg ac
//
LOCUS       DAORPM010000300         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_22462_length_1997_cov_15.100927, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000300 DAORPM010000000
VERSION     DAORPM010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22462_length_1997_cov_15.100927"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLD53_11460"
     CDS             complement(<1..251)
                     /locus_tag="PLD53_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRC61579.1"
                     /translation="MASILRLVEILWQFQPKLANRRTATTKFAQRFYTVWAGSRRTVN
                     WRTGSAIYWWRFTRPSPGTDRETAPKVGSPAIALATQFS"
     gene            complement(264..1694)
                     /locus_tag="PLD53_11465"
     CDS             complement(264..1694)
                     /locus_tag="PLD53_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023098450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HRC61580.1"
                     /translation="MKRFIEGGSRTQSTLLPECLDDYIAETNPVRVVDVFVDELALGK
                     LGFEGVEPAATGRPAYHPAVLLKLYIYGYLNRIQSSRRLEKESQRNVELMWLTGRLMP
                     DFKTIASFRKDNGKAIRNVCRQFVVLCQQLGLFSEALVAIDGSKFKAVNNRDRNFTSA
                     KLERRMQEIESSINRYLTELDTADRQEPAVAKVRGERLQDKIAALKEQMKALKEIEIR
                     LNDTPDKQISLTDADARSMKTRGTGIVGYNVQTAVDAKNHLIVAHEVTNIGIDRDQLT
                     TMAKEARAAIGAEELTVIADRGYFKGEEIVACHEAGIIAIVPKTTTSSAKANGRFDRA
                     DFIYAPEKNEYRCPADQALIWRCSTVERGLKLHRYWSSNCKQCAIKEKCTPGVERRIT
                     RWEREDILETMQDRLDQAPDSMRIRRQTVEHPFGTLKLWMGSAHFLTRTLDRVSTEMG
                     LHVLAYNLKRVMKILGSGALIEAMRA"
     gene            complement(1771..>1997)
                     /locus_tag="PLD53_11470"
     CDS             complement(1771..>1997)
                     /locus_tag="PLD53_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     aminotransferase BioA"
                     /protein_id="HRC61581.1"
                     /translation="MIAAFDVDTDDPYFSRRFYRAALDREALIRPIGNTVYLMPPYIV
                     SDAEIAHLGEAAQGALLEAISGKQGSSRNC"
BASE COUNT          400 a          562 c          573 g          462 t
ORIGIN      
        1 catgagaact gcgtggcgag cgcaatcgcg ggggatccga ccttcggggc ggtctcccga
       61 tccgtgccgg gtgaaggtcg cgtgaatctc caccagtaaa tcgctgaccc ggtgcgccag
      121 ttgacagttc gtctggaacc ggcccagacc gtgtaaaaac gctgagcgaa tttggtggta
      181 gcggtgcggc ggtttgccag tttgggttgg aattgccaaa gtatctcgac gaggcggagg
      241 atggaggcca aaaaaggacc ttctcaggcc ctcatggcct cgattagcgc cccgctaccc
      301 agtattttca tcacgcgctt aagattatag gcaagcacgt ggagtcccat ttcggtactg
      361 acccgatcca gtgttctggt caggaagtgc gcgctgccca tccataactt gagggtgccg
      421 aaggggtgtt ccaccgtctg gcgccgaatg cgcatgctgt caggtgcctg gtccaagcga
      481 tcttgcatgg tctcaaggat atcctcgcgc tcccagcgag taatccggcg ctctacaccg
      541 ggagtgcatt tctctttaat ggcgcattgc ttgcagtttg aactccagta acggtggagc
      601 ttcaagcctc tctcaacagt cgaacaacgc cagatcaacg cctggtcagc aggacagcga
      661 tattcattct tttctggggc gtaaatgaaa tctgcccgat caaagcgccc attggccttg
      721 gcactggagg tcgtcgtttt ggggacgatg gcaatgatgc cggcttcgtg acaggcaaca
      781 atttcctcac ccttgaagta gccccgatca gcaatcacgg tgagttcttc tgccccgatc
      841 gcggcacgag cctccttggc catggtggtc agttggtctc ggtcgatccc gatgttcgtt
      901 acttcatgcg ccacgatcag atggttcttt gcatccaccg cagtctggac gttgtagccg
      961 acgatgcccg taccccgagt cttcattgaa cgcgcatccg cgtcagtcag ggaaatctgc
     1021 ttgtcaggcg tgtcattgag gcggatctca atttccttga gcgctttcat ctgctctttc
     1081 aacgcggcga ttttgtcttg caagcgttcg ccccggactt tcgcaacagc aggttcttgc
     1141 cggtcagccg tatcgagttc ggtcaggtaa cgattgatgc tcgattcgat ttcctgcatt
     1201 cgccgttcca acttggcact ggtgaagttc cggtcacgat tattgacggc tttgaacttg
     1261 ctgccgtcga tggcgaccag ggcttcagag aacagtccca actgctgaca cagcaccaca
     1321 aactgacggc aaacattgcg gatcgctttg ccgttgtctt tgcggaaact ggcgatggtc
     1381 ttgaagtccg gcatcagacg ccccgtcagc cacatcagct cgacattgcg ttggctctct
     1441 ttctcaaggc gccggctgga ctggatgcga ttgaggtagc cgtagatgta aagcttcaga
     1501 agtacggcgg ggtgataggc cggccgaccg gtggctgccg gctcaacacc ctcgaatccc
     1561 agcttgccca gagccagttc atcgacgaag acatccacga cccgtaccgg attggtctcc
     1621 gctatgtagt cgtcaaggca ttccgggagc agcgtgctct gggttcggct tccaccttcg
     1681 atgaaacgct tcatggcggc cactcaataa acatgacaaa cgcactatat catggggcgt
     1741 tttgacacac tctgggccgt actgagccat tcaacaattg cgcgagcttc cctgtttgcc
     1801 ggaaattgcc tcaagcaacg cgccctgggc ggcttcgccg agatgcgcga tttccgcatc
     1861 actgacgatg taaggcggca tcaggtaaac cgtattgccg atcggccgga tcagggcttc
     1921 gcggtcgagg gcggcgcggt agaagcggcg ggaaaagtat ggatcgtcgg tgtcgacgtc
     1981 gaaggcggcg atcatgc
//
LOCUS       DAORPM010000301         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_23192_length_1959_cov_8.839286, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000301 DAORPM010000000
VERSION     DAORPM010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23192_length_1959_cov_8.839286"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLD53_11475"
     CDS             complement(<1..194)
                     /locus_tag="PLD53_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRC61582.1"
                     /translation="MKNMRAFEKPGRAIFLAVLLALAGCASRSPAPVFERGGQLSSAT
                     DKDSYAVKHGDTLYSIAREHG"
     gene            complement(191..847)
                     /locus_tag="PLD53_11480"
     CDS             complement(191..847)
                     /locus_tag="PLD53_11480"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRC61583.1"
                     /translation="MSQVLSGSGMTSQRTRSRMIERLREKGIRNERVLAALAAVPRHV
                     FIEEALASRAYEDTALPLGFSQTISQPFIVARMIEVLLEGRELGKTLEIGAGCGYQAA
                     VLAQLTKEIYAVERIEPLLAKAKLNLRAIQQFRVRLKYADGQLGLPEAAPFDTIIVAA
                     AASSVPPALLQQLALGGRMLLPVGKGEQYLLLVEHRAEGFVETRLDGVRFVPLLSGLE
                     "
     gene            complement(844..1587)
                     /gene="surE"
                     /locus_tag="PLD53_11485"
     CDS             complement(844..1587)
                     /gene="surE"
                     /locus_tag="PLD53_11485"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRC61584.1"
                     /translation="MRILLSNDDGYFSPGIECLARALSTVADITVVAPERDRSGASNS
                     LTLDRPLSLKRSANGFYHVNGTPTDCVHLAVTGMLDVLPDIVVSGINDGANMGDDTIY
                     SGTVAAATEGFLLGVPSLAISLCSKPGEHYDTAARVALDLVQLILRQNTLDPLLLNVN
                     VPDVPYEKLRGKVVTRLGKRHKAEAVVRTVTPRNETVYWVGAAGEAQDAGEGTDFFAV
                     ANNQVSITPLQIDLTHNAQLPLIRSWLAQ"
BASE COUNT          466 a          589 c          522 g          382 t
ORIGIN      
        1 ccatgttcgc gagcaatgct gtagagggta tcgccatgct tgacggcata actatccttg
       61 tcagtagcgg acgacagttg accgccgcgc tcgaatactg gtgcgggaga ccggcttgca
      121 catccggcca gagcgagcag aacggcaagg aaaatagctc tgcccggctt ctcgaaagcg
      181 cgcatattct tcattcaagt cctgagagaa gtggaacgaa acgaacacca tccagacggg
      241 tttcaacaaa gccctcggca cgatgttcaa ccagaagaag gtactgctcc cccttcccca
      301 ccggtaacag cattctgcca ccaagtgcga gttgctgcaa aagcgccggc ggaacggacg
      361 aggccgcagc ggcaacaatg atggtatcaa agggcgccgc ttcgggaaga cccaactgcc
      421 catcagcata tttcagacga acccggaact gctggattgc gcgcaagttg agcttggctt
      481 tggccagcaa cggctcgatc cgttcgaccg catagatttc ctttgtcagc tgcgccagga
      541 cagcagcctg atagccgcac cccgcaccaa tctcaagcgt cttgcccaat tcacgccctt
      601 caagcagcac ctcaatcatt ctcgccacga taaagggctg cgagatcgtt tgcgaaaagc
      661 cgagtggcaa ggccgtatcc tcgtaggcgc gcgaagcaag cgcctcttcg atgaacacat
      721 gacgcggcac cgcggcaagg gcggccaata cccgctcgtt acgaattccc ttctcacgca
      781 ggcgctcaat catgcgcgag cgtgtgcgct gcgaggtcat gccactgccg gaaagcactt
      841 gactcattgt gccagccagc tgcgaatcag aggaagctgc gcgttgtgcg tcagatcgat
      901 ctgcaatggc gtaatcgaga cctgattgtt ggccaccgca aaaaaatcag tgccttcgcc
      961 ggcatcctga gcttcgccgg cagcgcccac ccaatagacc gtctcgttgc gcggcgtcac
     1021 ggtgcgcacg accgcttccg ctttgtgacg cttgcccagc cgcgtcacga ccttgccacg
     1081 cagtttctcg taaggcacat ccggcacatt gacattgagc agcaaggggt cgagtgtatt
     1141 ctggcgcaag atcaactgca ccagatccag cgcgacacgc gctgccgtat cgtagtgctc
     1201 accgggcttg ctgcacaatg aaatcgccaa cgatggaaca cctaacaaaa acccctcggt
     1261 ggccgccgcg accgtacccg aatagatcgt gtcatcgccc atgttggcgc cgtcgttgat
     1321 gcccgaaaca acgatgtccg gcagtacgtc aagcatgccg gtcacggcca gatgcacaca
     1381 atcggtgggc gttccgttta catgatagaa accgttggcc gagcgtttga gggaaagcgg
     1441 acgatcgagc gtcagggaat tgctggcgcc gctgcggtcg cgttcaggtg ccacgacggt
     1501 aatgtccgcc accgtcgaca gcgctcgcgc cagacattca atcccaggcg aaaagtaacc
     1561 gtcatcgttg ctgagcaaaa ttcgcatatc aaaaccataa gagcggaaca agagtcacaa
     1621 aaaagccggc gattagccgg cttctggaaa atgctcggaa cgtttgagct gtctgacgac
     1681 cctttgcgtt tgccacctga aaccatgccg ccatcaatca actggaaatt atacgggtat
     1741 tcgcctccga ttgtgcatgc ggtgctcatt cagatcagca ctgtggaact ggctcaggga
     1801 taagctgtct cttgatgcat ccaagtcaat ttggatgccg aatgcgccag caccttgaac
     1861 attggtttcg gcaaaccctt cgcttagcga ttcagcctta gtattcacaa tccagccggc
     1921 cacttctcaa caccgacttc cggagagccc tatgaaaag
//
LOCUS       DAORPM010000302         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Propionivibrio aalborgensis isolate
            AMR_MDS_5395 NODE_26034_length_1829_cov_7.363585, whole genome
            shotgun sequence.
ACCESSION   DAORPM010000302 DAORPM010000000
VERSION     DAORPM010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867612
            Sequence Read Archive: SRR999554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Propionivibrio aalborgensis (wastewater metagenome)
  ORGANISM  Candidatus Propionivibrio aalborgensis
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Rhodocyclaceae; Propionivibrio.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:20:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,300
            CDSs (total)                      :: 2,285
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 15
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Candidatus Propionivibrio aalborgensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26034_length_1829_cov_7.363585"
                     /isolate="AMR_MDS_5395"
                     /isolation_source="Domestic wastewater"
                     /db_xref="taxon:1860101"
                     /environmental_sample
                     /geo_loc_name="Denmark"
                     /collection_date="2011-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLD53_11490"
     CDS             complement(<1..550)
                     /locus_tag="PLD53_11490"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HRC61585.1"
                     /translation="MMYARLTGTGSCLPGIPVSNADLAARGIDSDDEWIVSRTGIRSR
                     FLATPGTTSSELGLVAAQRALEMAGVAPSDIDLIIVATSTPDFIFPSTACLIQGRLGS
                     SKGAAAFDVQAVCSGFVYALGIAEKFIRSGSHSKALVIGAEVFSRILDWSDRGTCVLF
                     GDGAGAVVLEASEKPGILATAMH"
     gene            complement(547..1578)
                     /gene="plsX"
                     /locus_tag="PLD53_11495"
     CDS             complement(547..1578)
                     /gene="plsX"
                     /locus_tag="PLD53_11495"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRC61586.1"
                     /translation="MTARIAIDCMGGDHGPSVTVPASLRFLEDHPAASLILVGREEML
                     QPLLGKWSANSRLRVVHAAEVVEMHESPALALRNKKDSSMRVAINLVKNGEADACVSA
                     GNTGALMAISRFVMKMMPGIERPAICAAVPTAKGHTHMLDLGANVDCGPEHLLQFGIM
                     GAMLVAAMEHSDRPTVGILNIGEEEIKGNEAVKAAAELLRASDLNFIGNVEGDGIYKG
                     DADVVVCDGFVGNVALKASEGLAQMLGSSLRSEFKRNWLTKLAAVIAMSVLNNFKQRF
                     DHRRYNGAILLGLRGISVKSHGSADAFAFGYAVGRAYDAAENRVLERIAARIASVTDG
                     QVARVEESA"
     gene            complement(1633..1812)
                     /gene="rpmF"
                     /locus_tag="PLD53_11500"
     CDS             complement(1633..1812)
                     /gene="rpmF"
                     /locus_tag="PLD53_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRC61587.1"
                     /translation="MAVQQNKKSPSKRGMHRAHDFLSNPPLAVEPTTGEVHLRHHISP
                     NGVYRGKKVLKAKGE"
BASE COUNT          364 a          577 c          545 g          343 t
ORIGIN      
        1 catgcattgc tgtggcgaga atgcctggtt tttcagaagc ctcaagcact accgcgcccg
       61 ccccgtcgcc gaaaagcaca caggtacccc gatcgctcca gtcaagaatc cgcgaaaaca
      121 cctcggcgcc gataaccagc gccttgctgt ggctgcccga gcggatgaac ttttcggcaa
      181 ttcccagagc gtagacaaag ccgctgcaga cggcctggac gtcgaacgcg gcagcgccct
      241 tgctgctgcc cagcctgccc tggataaggc aggcggtact gggaaagatg aaatccggag
      301 tcgatgtcgc cacgatgatc aggtcaatgt cactgggcgc gacgccggcc atctccagcg
      361 cccgctgtgc agcgaccaga cccagttcgc ttgaggtcgt tcccggcgtc gccaggaagc
      421 gactgcgaat gccggtacgc gaaacgatcc actcgtcatc gctatcgatg ccgcgcgccg
      481 ccaggtcggc attgcttact gggataccgg gaaggcagct gccggtgccg gtcagtcgtg
      541 catacatcag gcgctctcct cgactctggc aacctgccca tcagtcaccg atgcgattcg
      601 tgccgcgatc cgctcgagga cgcggttctc ggctgcatcg taggcacggc caacggcgta
      661 gccaaaggcg aaagcgtcgg ctgatccgtg gctcttgacc gagatgccgc gcaggcccag
      721 caggatggcc ccgttgtaac ggcgatggtc gaagcgttgc ttgaaattgt tgagaactga
      781 catcgcgatg acggctgcca gcttggtcag ccagttgcgc ttgaactcgc tgcgcagcga
      841 tgaacccagc atctgcgcca gtccctccga cgccttcagg gcgacattgc cgacgaaacc
      901 gtcgcagacg acgacatcgg catcgccctt gtagatgccg tcaccctcga cattgccgat
      961 aaaattgaga tcggacgcgc ggagaagctc ggcggcggcc ttgaccgctt cgttgccctt
     1021 gatctcttcc tcgccgatat tgaggatgcc gaccgtcggg cgatcgctgt gctccatcgc
     1081 ggcgacgagc atggcgccca tgatgccgaa ctggagcagg tgttccggtc cgcaatcgac
     1141 gttggcaccg agatcgagca tgtgagtatg ccctttggct gttggcacgg ccgcgcagat
     1201 agccggtcgc tcgatccccg gcatcatctt catgacaaac cgtgaaatcg ccatcagcgc
     1261 gccggtgttg cccgccgaaa cgcaggcatc cgcctcgcca ttcttgacga ggttgatcgc
     1321 cacccgcatc gacgaatcct tcttgttgcg caaagcaagc gctggcgatt cgtgcatctc
     1381 cacaacttcc gcagcgtgga cgacgcgcag gcgcgaattc gctgaccatt ttcccaagag
     1441 cggttgcagc atctcctcac ggccgacgag gatcagactg gcggccggat ggtcttcgag
     1501 aaaacgcagg gacgccggca ccgtcaccga cggtccgtgg tctcctccca tgcaatcgat
     1561 ggcgatgcgg gctgtcatgg caaaataaaa ggcaggcagc ccggaacgga cagcctgccc
     1621 ttcattgaac gatcactcgc ccttggcctt gagaaccttc ttgccgcgat agacgccgtt
     1681 cggcgaaata tggtggcgca gatgcacttc gccggtggtc ggctcgacgg ccagcggcgg
     1741 attggacagg aagtcgtgag cgcgatgcat gccacgcttg gaaggggatt tcttgttctg
     1801 ttgaacagcc atgtcattac tccaaaaaa
//
